LOCUS       DASSFP010000001         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1638581, whole genome shotgun sequence.
ACCESSION   DASSFP010000001 DASSFP010000000
VERSION     DASSFP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638581"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFH91_00005"
     CDS             complement(<1..462)
                     /locus_tag="VFH91_00005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6861383.1"
                     /translation="MLTPDTVLQSRYKIIRQLGQGGMGAVYEAMDERLDTVVALKETL
                     FTDERLRKQFEREARLLARLHHPALPRVSDHFSEGEGQFLVMQFIPGDDLSEMMTRKR
                     GPFPADQVLTWADQLLDALDYLHTQEPQIVHRDIKPQNLKLTARGQIILLDF"
     gene            636..3461
                     /locus_tag="VFH91_00010"
     CDS             636..3461
                     /locus_tag="VFH91_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET6861384.1"
                     /translation="MAERFGKKPDIIDVGQRPRRRLFVWVLVGLGILLLLSSRVLSIY
                     LSALWFGSMGYSSVYWTIFKLKAGLFVGFALSTAILLSATFWLFQKLFGAAAFESRTI
                     LLNNQPFQFSPAKVIRPLGWVVAGLVGLVYGFAMKDEWRVFSLFFHQPAAPAADPIMG
                     KSLGFYLFSLPFYNSVSSWLTGVTFIILIAALAYSLLGLPQTVLKPSVRWSSGAAFRG
                     VCCALALFLIVLSWRVYLSRFPLLWEDHPIFSGVTYTEAHYTIPALFVVSVALVIAAL
                     ILLVNAFFVRRFSLVVIAIAIPLAAYLGGVVAVPSYVQSFVVKPNELDRESPYITNNI
                     EWTRRAFALENLELRDFEADATTAAVDPAANKDTLQNIRLWDWQALKDTLKQIQAIRT
                     YYDFSDVDVDRYTSSSQTHQMMIAPREIDDDKLPGRNWINERLIYTHGYGVTMNTSNG
                     FTPEGLPQFVLSNMPVESSAPEIKITRPQIYFGELTDRHVYVKTKQKEFDYPQGDSNT
                     YTTYDGTGGIQVGGTLRRMLLAWSVGDLSKLPFSDDVTSDSRLLINRNIRSIVDGVAP
                     FLIYDKDPYIVVSNDGRLYWIIDAFTESSSFPYSTHHIVGDNSVNYIRNSVKVVIDAY
                     NGSVNFYIFDKHDPIITAYRSVFPGLFKDNAEMPADIRSHIRYPETLIRAQAEVYGLY
                     HTQNPKVFFQREDVWNVAQQLTLSPEGKKISQPMDPYFVLMQLPGEQQRNEFVLILPF
                     TPSNRNNMIGWMAGRSDGDNYGKLLVYNFPKSRLIDGPVQIEARIDQNAQLSGQFTLW
                     NQQGSRVIRGHLLVIPIGKSLLFVEPIYLQAVQSPMPELRIVVLATQDKLAYGQTFAE
                     AMTNLFGEAGKAPEEQKPQSGTEQKPTESTAQPSTATVQQLVIKALQEFEDYQRLTSQ
                     GKLSEAGQKLEQHKRTLEELRKLAPKQ"
     gene            complement(3536..4363)
                     /locus_tag="VFH91_00015"
     CDS             complement(3536..4363)
                     /locus_tag="VFH91_00015"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET6861385.1"
                     /translation="MNIITKKLRKTPEMLVTIGTILGLFLVVIASTSCQNAPSAHEVV
                     DRGDEKIATDRIKEGDGLYEARADLSKARVAVAALRQAQLADYGNYEAAWKLSRASFY
                     VGDHTDNDAERDDMFRAGTEAGKAAVKLQPNKPEGHFWLGANYGGAASHSTLASLSSV
                     QDIRGEMEAVIKLDEGYQGASAYLGLGRLYLQAPKVLGGDTGKAIESLLKGLKMSPNN
                     SLMRYYLAEAYANSNRDADAKKELETLANTTPDPKHMPEHQDALAKAKKLETKLRPS"
     gene            4498..>4720
                     /locus_tag="VFH91_00020"
     CDS             4498..>4720
                     /locus_tag="VFH91_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861386.1"
                     /translation="MAGAAFYDLEGTLVSTNLVHTLSFYARRQQGLVSSFTKSATTLL
                     SLPVFAVTDQYSRKVFNDLYFKRYKGQSED"
BASE COUNT         1143 a         1206 c         1135 g         1236 t
ORIGIN      
        1 aaagtcgagg agaatgatct gaccgcgcgc ggtcaacttc aggttctgag gtttgatatc
       61 gcgatgaaca atctgtggtt cctgggtgtg caggtaatca agagcatcaa gcagttgatc
      121 ggcccatgtt aaaacttgat cagccggaaa aggaccacgt ttacgggtca tcatctcgga
      181 aagatcatca cccggaataa actgcattac gagaaactgt ccttcacctt cagaaaagtg
      241 gtcactaact cgcgggagcg caggatgatg aagacgagcc aacaatcgcg cttccctttc
      301 gaactgcttt cgcaatcgtt cgtcggtgaa aagggtttct tttagcgcca cgaccgtatc
      361 caggcgctca tccatcgctt cgtaaacggc gcccatgccg ccctgaccca gttggcgaat
      421 aatcttatat ctactctgta aaaccgtgtc tggcgtcagc attaacagtc tttcttattc
      481 gtactactaa ccatttgacg ttggtgaagc aatcagggcc gaataagtgc aggtcaaaaa
      541 ctaagcaaag tcattataca cagcgatcca gttccgcaaa atctaatcac cctggaatca
      601 ttgagggaac cactggttgc gggtatgcta cgagcatggc agaaagattt ggcaaaaaac
      661 ccgacattat cgatgtcggc caacgccccc gcaggcgact cttcgtctgg gtgctggtcg
      721 gcctcggtat cctgctctta cttagttcac gcgttctctc aatttacctg tcagcgttgt
      781 ggtttggctc gatgggttac tcgtccgtct actggacaat ctttaagttg aaggctggcc
      841 tttttgttgg ctttgcgttg tcgacggcca ttcttctgag cgcgactttc tggctcttcc
      901 agaaattgtt tggcgcagcg gctttcgaaa gccgtacgat tctgctaaac aaccaaccat
      961 ttcagttttc gccggcaaaa gtcataagac cactgggctg ggtcgtagcg ggactcgttg
     1021 gtctggtcta tggattcgcg atgaaagacg agtggcgggt attctcgcta ttctttcatc
     1081 agcctgcagc cccagctgct gatccaatta tgggcaagtc tctgggtttc tatctgttct
     1141 cacttccctt ctacaactcg gtgagttcat ggctcaccgg cgtgactttc attatcttaa
     1201 ttgccgcgct cgcttattcc ttactcggac ttccacaaac ggttttgaag cccagcgtca
     1261 gatggtcatc aggcgcggcg tttcgtggtg tttgttgcgc gttggccctg ttcctgatcg
     1321 tgttgagttg gcgcgtgtat ctgtcccgtt ttccgctcct atgggaagac catccgattt
     1381 tctcgggcgt cacctatacc gaagctcact acacgattcc ggcgctcttt gttgtctcgg
     1441 ttgcgctcgt catcgctgcg ctgatcctgc tcgttaatgc atttttcgta aggcgattca
     1501 gcctggttgt gatcgcgatt gcaattccac tcgcagctta cctaggtgga gtcgtggcgg
     1561 ttccatctta tgttcagagc ttcgtggtga aaccaaacga gttggatcga gagtcgccct
     1621 atattacgaa caatatcgaa tggaccagac gagcattcgc tctcgaaaat cttgaactac
     1681 gagactttga agctgatgca acgaccgccg cagtcgatcc cgcggctaat aaagatacgt
     1741 tacagaacat ccggttgtgg gattggcagg cgctgaaaga cacgctgaaa cagattcaag
     1801 cgattcgtac ctattatgac ttttcggacg tcgacgtcga tcggtataca agctcatcgc
     1861 aaactcacca gatgatgatt gcgccgcgtg agatcgacga cgataagtta cccggaagaa
     1921 attggataaa tgaacggctg atctacaccc acggatacgg ggtgacgatg aatacgtcaa
     1981 acgggttcac tcccgaaggt ttaccacaat tcgttctgtc gaacatgccg gttgaatcaa
     2041 gtgcaccgga gatcaagatc accaggcccc agatctattt tggcgagtta actgatcgtc
     2101 acgtttacgt taagacaaaa caaaaagagt ttgactatcc gcaaggagat tcaaacacat
     2161 atacaaccta cgacggcaca ggcggcattc aggtaggagg caccttaagg agaatgctac
     2221 tggcgtggtc cgtcggcgat ttatccaagc ttccattttc cgacgatgtt accagtgata
     2281 gtcgcctgct aatcaatcgg aacattcgct caatagtcga tggtgttgct ccgtttctta
     2341 tttacgataa agatccctat atcgtggtca gtaacgacgg gcgactgtac tggatcatcg
     2401 acgcttttac agagtcatcg agctttccat attcaactca tcacatagtc ggagacaact
     2461 cggttaatta cattcgcaac agcgttaaag tcgtcatcga cgcctataac ggatcggtca
     2521 acttctacat tttcgataag cacgatccaa tcatcaccgc ttacagaagt gtctttcccg
     2581 ggttgtttaa agacaatgct gagatgccgg cggatatccg gtcccacata cgttatccgg
     2641 agacgttaat tcgagcgcaa gccgaagttt atggcctcta tcacactcaa aatcccaagg
     2701 tgttctttca acgcgaagat gtatggaacg ttgcgcaaca gcttacgctt agccctgaag
     2761 gtaaaaaaat atcgcagcca atggatccct attttgtgtt gatgcaatta ccgggtgagc
     2821 agcagcgcaa tgaatttgtt ctgatacttc cgttcacgcc ttcgaaccgt aataacatga
     2881 ttggttggat ggcaggccgg agcgatggtg acaactatgg caagttactg gtttataact
     2941 ttccgaagtc acgactgatt gatggtcctg tgcagattga ggcgcgcatt gatcagaatg
     3001 ctcaactatc ggggcagttc acgttgtgga atcaacaggg atccagagtt attcgcggtc
     3061 atctactcgt gataccgatc ggaaagtcac ttttgtttgt ggaaccaatt tacctgcaag
     3121 cggtccagag tccgatgcct gaactgcgaa tcgtcgtgct ggcaacccag gacaaattgg
     3181 cgtatggaca aacgtttgct gaggcaatga caaatctgtt tggagaagcg ggcaaagcgc
     3241 ctgaagaaca gaagccacag tcaggcaccg aacaaaagcc cactgaatca acggctcaac
     3301 cttcgacggc aactgtccaa cagttagtta tcaaagcgct gcaggaattt gaggactatc
     3361 agcgtctgac ctcgcaggga aagctatccg aagcgggtca aaaactggag caacataaga
     3421 gaactttgga ggagttgcgt aaattggccc cgaaacaata gttagagaaa ctatcgctga
     3481 taccgcgcgg ctttagccgc gtggtccttc aacttccacc tagcccgtca ctcttctaac
     3541 ttgggcggag ttttgtctcg agtttttttg ctttggccaa cgcatcctgg tgttccggca
     3601 tatgctttgg atcgggagta gtgtttgcca gggtttccaa ctctttcttc gcatccgcat
     3661 cgcgattcga attcgcataa gcctcagcca ggtaatatct catcaacgag ttattcggac
     3721 tcatcttaag tcccttcagc aaactctcaa ttgccttccc cgtatcgcct ccaagaacct
     3781 ttggcgcctg taaatagagc cgacccaggc ccagataagc actcgccccc tggtagccct
     3841 cgtctaactt gattactgct tccatctcgc cgcgaatgtc ctggacgctc gacaaactcg
     3901 ccagcgtgct gtgcgacgca gcgccgccat agttggctcc cagccaaaaa tggccctccg
     3961 gcttattggg ctgaagctta accgccgctt tcccagcctc agtgcccgca cgaaacatat
     4021 cgtctcgctc ggcatcatta tcagtgtggt ctccaacata gaaggaagca cgcgagagtt
     4081 tccaggccgc ctcgtagttt ccgtagtctg caagttgagc ctgtcgaagt gctgcaactg
     4141 ccactctggc cttagaaagg tccgctcgcg cttcgtaaag gccatctcct tccttaattc
     4201 gatcggtggc gattttttcg tcgccacgat caactacctc gtgcgccgag ggtgcgttct
     4261 gacatgaagt actggctatc accaccagaa acaatcctaa aattgttcct attgtgacca
     4321 gcatttccgg tgttttcctg agctttttag taattatgtt catgattgtc tggttacgct
     4381 atacagtttg taatagtttt gctacggcgt tatctaaatc gggctgaatg gggtatattg
     4441 acccgactga agtcaaaatt ttgcagtcgg tcttttaggg tcaacggaga cagaaacttg
     4501 gcaggggcag ctttttacga tttagaggga accttagtca gcactaacct tgtccatacc
     4561 ctgagcttct acgccaggcg acagcaaggt ctcgtcagta gtttcacaaa gagtgctacc
     4621 acactgctga gccttcccgt cttcgccgtt actgaccagt atagccgaaa agtgttcaat
     4681 gacctctatt tcaaacgcta caaaggccaa tcagaagatc
//
LOCUS       DASSFP010000002         8744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1787809, whole genome shotgun sequence.
ACCESSION   DASSFP010000002 DASSFP010000000
VERSION     DASSFP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787809"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      368..476
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            549..2462
                     /gene="thiC"
                     /locus_tag="VFH91_00025"
     CDS             549..2462
                     /gene="thiC"
                     /locus_tag="VFH91_00025"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET6861387.1"
                     /translation="MSSSVREPDPDVVTTLPNSRRVYINGKQGGVRVPFREISQTPTR
                     SFDGSLEENSTVTVYDTSGPWGDPSQRCNVTEGLPTLRRHWILERNDVEEYSGREAKP
                     IDDGYLSVEAAARARVRDVGKLEDFPGLRRAPLKAKPGKTVTQLHYARRGIITPEMEF
                     IAIRENLGRALAHNASDDRSSLDHQHPGQSFGADLPSFVTPEFVRDEVAYGRAIIPAN
                     INHPESEPMIIGRNFLVKINANIGNSSIASSIDEEVDKMRWSIRCGSDTVMDLSTGKN
                     IHATREWIIRNSPVPIGTVPIYQALEKVSGKAEELTWEMFRDTLIEQAEQGVDYFTIH
                     AGVRLPYIPLTAKRTTGIVSRGGSIMAKWCLAHHEESFLYTHFREICEIMAAYDVSFS
                     LGDGLRPGSIADANDDAQFAELDTLGELTKIAWEYDCQVMIEGPGHVPMHLIKENMDR
                     QLAICHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNK
                     KDVKDGVIAYKLAAHAADLAKGHPGAQYRDNALSKARFEFRWEDQFNLSLDPEVAREF
                     HDETLPQEGAKLAHFCSMCGPHFCSMKITQDVRDYAAQKAIDEQAALEIGMKEKAEEF
                     VSAGGELYRSSTT"
     gene            2723..4519
                     /locus_tag="VFH91_00030"
     CDS             2723..4519
                     /locus_tag="VFH91_00030"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET6861388.1"
                     /translation="MKSLFNLFVSILLITNFGLSNLMAQGKSTPAEQRIKQLHELLAE
                     QWEYTLKSSPEFASILGDRRYNDKLTDYSEAAIAKDLDQSRAFLKRFESINTAEFPEL
                     ERLNRDLMVLNLRDRLDSEKFKDWEMPVTQFGGIHLDMPQLVTALPFTTIKDYDDYIA
                     RLKQLPRAFEQITLLMQKGMKDGLMPPKILLTQVAEQTEGIAKQKPEDSPFAQPTTAF
                     PSTFSTGDKERLRLGVLDAIRNSALPAYNQFLTFVRDEYAPKGRTEIGISSLPLGQER
                     YAAAIRRQTTTNMTAEEIHQLGLKEVARIEAEETDVARQLGFKDLRSFKTSLKTNPAV
                     HPKSRQQILGEYQTYTDQMWKKLPALFGRLPKAKLEILPVEAFREKTASTSYVQGTPD
                     GSRPGHVFVNTYDFENQLTINNEDTAYHEGVPGHHMQISIAQELTDLPPFRQQAGYTA
                     FVEGWALYSERLGKDVGFYQNPYSNYGRLDDEMWRAVRLVVDTGIHYKHWTRDQVLQY
                     MRDHSSANEAYFQSETDRYIAWPGQALAYKIGQLTILRLREKAKAELGDRFDIRGFHD
                     EVLGAGALPLDVLERRVNGWIEQSRVSKSTSN"
     gene            complement(4512..5462)
                     /locus_tag="VFH91_00035"
     CDS             complement(4512..5462)
                     /locus_tag="VFH91_00035"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6861389.1"
                     /translation="MPSHEMTYRTLGGTGERVSAIGLGGWHLGLKHVDEQLAFRIVRS
                     AIDRGMTFMDNCWDYNEGASETRMGKALEDGYRDKVFLMTKIDGRSKKEATRQLDESL
                     KRLKVDMIDLVQHHEILRFEDPHRIFDMEGANAALLEAKEGGKLRYIGFTGHKDPHIH
                     LHMLRVAAEHRFKFDTVQMPLNVMDAHYRSFEKLVLPELVQENIGVLGMKCMGNGILL
                     KSKKVKPMECLQYALNLPTSVVITGIDSMAILDQAFEAASTFKRLTGEQVDALLARTS
                     DVALTGEFEPFKTTSIFDGTAGHPEWLGEEPARVQQLMLN"
     gene            5654..6175
                     /locus_tag="VFH91_00040"
     CDS             5654..6175
                     /locus_tag="VFH91_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6861390.1"
                     /translation="MTILETERLTIRELNDGDAPFILQLLNEPSFIYYIGDKGVRTLE
                     DARQYILNGPVISYERNGFGLNLVSFKETSTPIGMCGLLKRDELPDPDIGFAFLPEYW
                     SKGFGFEAASAVLKYGFEVLRLQRILAITSPDNEGSGRLLTKIGLKYEQMYSLSDDRP
                     EVKLFVIDFGEKA"
     gene            6172..6555
                     /locus_tag="VFH91_00045"
     CDS             6172..6555
                     /locus_tag="VFH91_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6861391.1"
                     /translation="MKLKLIHQVAMLSNDLDETISFYRDKLGARFVARFDPPGLVFFD
                     VNGTRLLFEKAAAKATVYFRVDDIDSAYRELLEHGVVFVDQPHAIFRDEQGTFGQPGE
                     EEWLAFFHDPSENLLALATRKKTSS"
     gene            6552..6947
                     /locus_tag="VFH91_00050"
     CDS             6552..6947
                     /locus_tag="VFH91_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET6861392.1"
                     /translation="MKKQTTKGPAPKPPEGDKHVERVRKFCLSLPETFEKLSHGEPTF
                     FVRKRVFAMCSINHHNDGHIAVTVPAAIGVQAALIESSPKKFYRPPYVGGAGWVGIEL
                     PRVSDRELKLHLQEAWKLIAPEKIHSLLK"
     gene            complement(6949..7668)
                     /locus_tag="VFH91_00055"
     CDS             complement(6949..7668)
                     /locus_tag="VFH91_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET6861393.1"
                     /translation="MVLKDSEAEHVSEVEAALLDADLFVKYRSFDRAVKTIKTALEHN
                     PRSLQLRERLREICASQKQGEEAARQCLALASIYIEREDFDTAHDRLLEAKQFDSRIN
                     IAAGLDAIRRARRPDLKTVPVAAEAHFEQRSFTLAGDMSVISVFDAIQVIENARISGI
                     LSMKNETHSAKIFFNFGTIVDAQAENENGEKAFQILVEITGGTFEFHKTEDQYPVKIQ
                     ASSNTNLILDTLRQLDEAKLQ"
     gene            complement(8491..>8744)
                     /locus_tag="VFH91_00060"
     CDS             complement(8491..>8744)
                     /locus_tag="VFH91_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861394.1"
                     /translation="TFSLDLNVSREFRISERLVIRPVVEFDNVLNKTVFSFGSEFVDF
                     KALASTATEEQHQAFLESFLLATRTMRPRQIRVGVKIDF"
BASE COUNT         2280 a         2177 c         2164 g         2123 t
ORIGIN      
        1 aggaggagca ctttgcagtg agactactga accaagatcc taacggtttg ctggttcaga
       61 ggtcaggccg cagccacctc actgtgataa gaactcatat atttttgaat cacctccttt
      121 ccagtgttgc ggcgatcata aagtacaagt gcatgagcgt caaccgcttt ttttttcagc
      181 tcttaattta tgctttcgag aatggtcaac tccactgtgg ttagcggtga cttcaaactc
      241 atacaccgca gaaagaaaga ccactaacca ccgagacaca aagaacacta aggatgcaca
      301 gagattagct gtcgaaacca ttatgcgctg aacaactccc atcggatgta gtagactacg
      361 actcacaatt tagtttcggg ggagccggga ggctgagagt ccagacgttt gatgtttgga
      421 gaccccttga acctgatgcg gttagcaccg tcgaagggag aaatggcgat tcataagcgc
      481 tccaaccttt ccttcaggtg gagcgttttt tcttaggcat tgatctcgtc ttatcggaga
      541 aacaaatcat gagtagctca gtgcgagaac cagaccctga cgtagtcaca actcttccaa
      601 actcacgtcg tgtttatatc aacggcaaac aaggtggcgt gcgtgtaccc ttccgcgaga
      661 tcagtcagac tcccacgcga agttttgacg gaagtctgga agagaattcc acggtgaccg
      721 tatatgacac aagcggcccc tggggcgacc cgtcgcagcg ctgcaacgtc accgaagggc
      781 tgccaacact caggcgacac tggatcctcg aacggaacga tgtcgaagag tactcgggtc
      841 gcgaagccaa gcccatcgat gatggttatc tgtcagtcga agctgcggct cgtgctcgcg
      901 taagggacgt tggcaagctg gaggactttc cgggattgcg ccgagctcca ttgaaagcga
      961 agccaggaaa aaccgtcacg caattgcact acgcgcggcg cggcatcatt actcccgaaa
     1021 tggagttcat cgcaattcga gaaaatctcg gccgcgctct tgcgcataac gcatccgacg
     1081 accgttcatc gctggatcac cagcatccgg gccagtcgtt cggggccgac cttccttcgt
     1141 ttgtcactcc ggaatttgtt cgagacgagg ttgcgtatgg tcgggcaatc atccctgcaa
     1201 atataaacca cccggaatcc gagcccatga ttatcggtcg caattttctg gtgaagataa
     1261 atgcaaacat cggcaattcg tcgatcgcct catcgattga cgaggaagtt gacaagatgc
     1321 gttggtccat tcgttgcggg tctgacacag tgatggactt atccaccggc aaaaacatcc
     1381 acgcaacccg cgaatggatt attcgaaatt ctccagtgcc gattgggaca gttccaattt
     1441 accaggcact cgaaaaagta agtggaaagg cagaggaact cacctgggag atgttccgcg
     1501 acacattgat cgaacaggca gagcagggtg ttgactactt tacgatccac gccggtgtca
     1561 ggcttcccta tattccattg acggcgaaaa ggaccaccgg catcgtaagt cgtggcggtt
     1621 cgatcatggc caagtggtgt ctggcccatc atgaggagag cttcctttac acccatttca
     1681 gagaaatctg cgagatcatg gctgcgtacg acgtatcgtt ctcactcggc gacgggctgc
     1741 gtcctggatc gatcgccgac gcgaacgacg acgcgcaatt cgccgaactg gacacgctcg
     1801 gagagctaac gaagatagcg tgggaatacg actgccaggt tatgatcgaa ggccctggcc
     1861 acgtgccgat gcatttgatc aaagagaaca tggaccggca attggcaatt tgccatgaag
     1921 ctcctttcta tacgctcggc ccactgacga ccgacatcgc gcccggttac gaccacatca
     1981 cctcagcgat tggcgccgca atgattgggt ggttcggaac tgcaatgctg tgctatgtca
     2041 ctcccaaaga gcatttggga ctacccaaca agaaagatgt gaaggacggc gtcattgcct
     2101 ataagttagc cgcacacgca gcggatctcg ccaaaggtca tccgggtgct caatatcgcg
     2161 acaatgcgtt atccaaggca cgttttgaat tccgctggga agatcaattt aacctttctc
     2221 tcgatccgga agttgcgcgt gagtttcatg acgagacctt accgcaggaa ggcgcaaagc
     2281 tggcacactt ctgctcgatg tgtggtccac atttctgttc catgaaaatc acgcaagacg
     2341 tccgtgatta tgcggcgcaa aaagcaatcg atgaacaagc cgcgctcgag attggcatga
     2401 aagaaaaggc tgaggagttt gtaagtgccg gaggcgaact ttaccggtcg tcgaccacat
     2461 aagatcacat acaacgtaga tcgggcggat gcaatcgaat gctctgatga tgtgggcttg
     2521 catgtctcaa ctctcaccgg gcttcaactg gtggaaagat caagcacaat ttggcaggga
     2581 ccgatttaac ggttttccca gcctcaggga taagaaaccg ttgaaacggt ttcgcgtcgc
     2641 tatttggggc tcaatcacct ggctgaagcc aggtgagaat gagacgtaag cgggagtttt
     2701 atggaatagt cggaggttag ttgtgaagag cttgttcaat ttgttcgtgt cgatcttgtt
     2761 aattacaaac ttcggtctct ccaatttaat ggcccagggc aaatctactc cagctgaaca
     2821 gcgtatcaag cagctacatg aactcctcgc cgaacagtgg gaatacaccc tcaaatcaag
     2881 tcctgagttc gcatcgattc ttggcgaccg ccgctacaac gacaaactaa ccgactactc
     2941 ggaagctgct attgcgaagg atctagacca gtcacgcgca tttctgaagc gcttcgaatc
     3001 aatcaatacc gccgagtttc ccgaactgga acgtctaaat cgagatctaa tggtcttgaa
     3061 tcttcgagat cgattagaca gcgagaagtt taaggactgg gaaatgcctg taacgcaatt
     3121 cggtggcatt catctcgata tgccacaatt agtgacagca ctcccgttca cgacgataaa
     3181 ggactacgac gactatatcg ctcgacttaa acagcttcca cgtgcgttcg agcagatcac
     3241 actcttaatg caaaagggta tgaaggacgg cttgatgcct cctaaaatat tgctgaccca
     3301 ggtcgccgaa caaaccgaag gcatcgcaaa acaaaaaccg gaagactctc cgttcgctca
     3361 gccaacaacc gcatttccgt caaccttttc aaccggtgac aaggaacgat tacgtctcgg
     3421 agtcctcgac gcaattcgca attcagcgct gccggcttac aaccagtttt tgacttttgt
     3481 ccgggatgaa tatgcgccca aaggacggac tgaaatcgga atctcgtcgc tccctttagg
     3541 acaggaaaga tacgctgcag cgattcgacg tcaaaccaca acgaatatga cagcggaaga
     3601 gattcatcag ctcggactaa aagaagtcgc acgtatcgaa gccgaagaga ccgacgttgc
     3661 ccgccagttg ggattcaagg atctccgatc cttcaagacc tcactgaaaa caaatccggc
     3721 tgttcatccc aagtcgcgac agcaaatcct gggcgagtat cagacttata ccgatcagat
     3781 gtggaagaag ctgcccgcac tctttggccg gctccctaaa gcaaaactgg aaatactgcc
     3841 tgtcgaagca ttcagagaga agacggcgtc tacttcttat gtccagggaa cgccggacgg
     3901 ttcaaggccc ggccacgtgt ttgtaaacac ttacgacttc gaaaaccagc tgaccatcaa
     3961 taatgaagat actgcttatc acgaaggggt ccccggccat cacatgcaga tctcgatagc
     4021 tcaggaactg accgaccttc ctccattcag gcagcaagcc ggctacacag cgtttgtcga
     4081 aggttgggca ctttactcgg aaaggcttgg taaggatgtt ggtttttacc agaatccgta
     4141 cagtaattat ggtcggctgg acgatgagat gtggagggcc gtcaggctag tcgtcgatac
     4201 gggcatacat tacaagcatt ggaccaggga tcaggtgctc cagtacatgc gcgaccattc
     4261 aagcgcaaac gaagcttatt ttcagtcaga aaccgatcgt tacattgcgt ggcccggtca
     4321 ggctttagct tacaagatcg gccaactgac aatcctgcgt ttgcgagaga aagcgaaggc
     4381 tgaacttggc gaccgattcg acatccgagg attccacgat gaagtactcg gtgcgggcgc
     4441 acttccgctc gatgtgctcg aacgtcgcgt caatggctgg atcgagcaat ccagggtttc
     4501 aaaatcgacg tctaattaag cattaattgt tggacccttg ccggttcttc gcccagccac
     4561 tccggatgcc cggccgtacc atcgaagata gatgttgtct tgaatggctc gaactccccg
     4621 gtaagtgcaa cgtccgaagt cctggccaac aaagcgtcga cctgctcgcc agtcaaacgt
     4681 ttgaatgtac tcgcagcctc aaaagcctga tcgaggattg ccatactatc gataccggta
     4741 atcactactg aggtgggaag atttagcgcg tactgcaaac actccatcgg ttttactttt
     4801 tttgatttca acagaattcc gttacccata catttcatgc ccagcacgcc gatgttctcc
     4861 tggaccagct ctggcaaaac gagcttttca aaacttcggt aatgggcatc catcacatta
     4921 agcggcattt ggaccgtgtc aaatttgaac cggtgttccg ccgccactcg caacatgtga
     4981 agatggatgt gcggatcctt atgtcctgtg aaaccaatgt agcggagttt gccgccctcc
     5041 ttagcctcga gtagtgctgc gtttgcgcct tccatatcaa aaatccgatg ggggtcctcg
     5101 aagcgcagaa tttcgtggtg ctgtacaagg tcaatcatgt cgacctttag tcgtttcaaa
     5161 gattcatcga gctgacgagt cgcttctttt tttgaacgac cgtcgatctt agtcattaag
     5221 aagactttgt ctcgatagcc gtcctcaagt gcttttccca tgcgagtttc gcttgcgccc
     5281 tcgttgtaat cccagcagtt gtccataaaa gtcatgccgc gatcgattgc tgatctgaca
     5341 atgcggaagg ccaactgttc atcaacgtgt ttgagtccaa gatgccaacc accgaggccg
     5401 atggctgaaa ctcgttcccc tgtaccacca agggtgcggt aggtcatttc atggctgggc
     5461 ataatcgtgc tccaaattcc tttagtttca gggatcaaac cagaacaatt tcgcgaaatc
     5521 cgttcgaaaa tgtccagaca atgctgaatc gtgatttcct gagtcgcgta gctactgatc
     5581 tctaaaggcc acatgctttg cccggtgacc aggtatcttg ctttagatta aaatagcccg
     5641 cccttacaac tatatgacca tactcgagac agaacgcctg acaattcgcg agctgaatga
     5701 cggtgacgca ccgtttatcc tccagctgct aaacgaaccc tcgttcattt attacatcgg
     5761 agacaaagga gtgagaactc tcgaagatgc acgtcagtac attctgaatg gaccggtcat
     5821 cagttatgag cgtaatggtt ttggtctcaa tctcgtgtcc tttaaagaga cttcgactcc
     5881 catcggaatg tgcggattgc taaagcgtga cgaacttccg gatccagaca ttggctttgc
     5941 ttttcttccc gaatactggt ccaaaggttt cggctttgag gcagcttccg cagtactcaa
     6001 atacggcttc gaggtcttgc ggcttcaacg catactggca atcacgtctc ctgacaatga
     6061 aggctccgga agactgctca ctaaaatagg gttaaagtac gaacagatgt attcgctctc
     6121 ggatgatcgc ccagaagtaa aacttttcgt tatagacttc ggagaaaagg catgaaactg
     6181 aaactaatac atcaggttgc aatgctttca aacgacctgg atgaaacaat ctcgttttat
     6241 cgtgacaagt taggagccag gttcgtcgcg cgtttcgatc cgcccggact cgtgttcttt
     6301 gatgtcaatg gaacgcgcct gttatttgaa aaggctgctg ccaaagcaac cgtctacttt
     6361 cgggttgacg atatagattc ggcttatcgc gaattactcg aacatggagt tgtattcgtg
     6421 gatcaaccac atgcgatctt cagggatgag cagggtactt ttggacaacc tggcgaagag
     6481 gagtggttgg cttttttcca tgatccttcg gagaacttac ttgctttagc cacacggaag
     6541 aaaaccagct catgaaaaag caaacgacta agggtccagc tcccaaaccg ccagagggag
     6601 ataagcatgt cgaacgagtg agaaagttct gcctttcact accagagacg tttgaaaagc
     6661 tctcgcatgg tgagccgacc ttttttgttc gcaagagagt tttcgcaatg tgctcaatca
     6721 atcatcataa tgatggccac atagcggtga ctgttccagc agctattggt gtgcaagctg
     6781 cattgatcga atcctcacca aagaaatttt ataggccacc ctatgtcggt ggtgcgggat
     6841 gggttggtat cgaactccca cgtgttagcg acagggaatt aaagctgcac ttacaggagg
     6901 catggaaact gattgctcct gaaaaaatcc actcgttact gaaatgagtt actgcagttt
     6961 agcttcgtcg agttggcgaa gtgtatcgag gatgaggttg gtgtttgatg atgcctggat
     7021 tttgaccggg tattgatctt cagttttatg aaattcgaag gtgccacccg ttatctcgac
     7081 taagatttgg aatgctttct cgccattctc gttttcagct tgcgcatcga caatggtgcc
     7141 gaaattgaaa aatatcttgg ccgaatgcgt ttcattcttc atcgacaaaa tccccgaaat
     7201 acgagcgttt tcgatgacct gaatcgcgtc gaagacactg atgacactca tatcacccgc
     7261 cagggtgaaa gatctctgct caaaatgcgc ctcggcagca acgggtacag tttttaggtc
     7321 aggccggcga gcgcggcgaa ttgcgtcgag gccggccgca atatttattc gcgagtcgaa
     7381 ctgcttggct tccagtaacc ggtcgtgtgc cgtatcaaag tcttcgcgct caatataaat
     7441 actggccagg gccaaacatt gtcgggctgc ttcttcacct tgcttttgag aggcacaaat
     7501 ctcacgcagc cgttcacgga gctgtagtga tcgaggatta tgctctaagg cggttttgat
     7561 tgttttgacg gcacgatcga acgatcggta cttcacgaac aggtcggcat caagtaatgc
     7621 ggcctcgacc tcgctaacgt gttcggcttc ggagtctttt agaaccattc cagtaattta
     7681 atctcccagc tttaagggag tttatcacag cattttaggc acctcgcgct ttcgattctt
     7741 acaagagctg cagctccttc gactttataa tcgccctgta ctgagggcat tcgagcgctc
     7801 ggcacaccta aaactcctgg tttttgcggt tggaaggtac aaaattcgtc gaagcggtgt
     7861 tccatttggt gtgtttttaa cgcgttacca gatagggaaa gttatcaaaa gcgggctttt
     7921 cgcattccaa cgagctggca cggaagctgc ggttaaaaga aagcaagttt ttgaaagacc
     7981 cgaggcaaac agtaagggac catgctcatc tgactggtca gaaagtgaaa ggcaacggaa
     8041 atgaatcgct ggatgaaaag acgaattgag aaatcccggg cccgctgcgt acaggcaagt
     8101 ggacgtaagg aacgaactga taatcgaatc aaggttgaag aagagaaccc cctggagtcg
     8161 cgaaagcaga gtattaggct cgagtcgcta ccgagccaaa gctaaagtcc ggtatttttc
     8221 cggtctattg atcctctcct cctcggtcag actcaccagg aagtaaaacc tcgaaacaga
     8281 agtcagttgg ggcgcggttg tttcggtaga gtccaattaa tgctcaatta tcatttgata
     8341 tttgagaatt gttaattggg tagaccttgg gtagacggcc cgcatcgcta tcaggcccaa
     8401 aggatggcgc agaattaaca attctcaaat ttgaaatcat aattgatcat tgattcggct
     8461 cactccaaac tgttcctttc taataactcc ttaaaaatcg atcttcactc cgacgcgaat
     8521 ttgtcgcggt ctcatcgtgc gcgtggcgag tagaaacgat tcaagaaacg cctgatgctg
     8581 ctcctctgta gccgttgagg ctaacgcttt gaaatcaacg aactccgatc caaaactgaa
     8641 taccgtttta ttcagaacat tatcgaactc tacaactggt cgaatcacga gccgttcact
     8701 tattcgaaat tctcggctta cattcagatc gagcgagaaa gttg
//
LOCUS       DASSFP010000003         5136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1638991, whole genome shotgun sequence.
ACCESSION   DASSFP010000003 DASSFP010000000
VERSION     DASSFP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638991"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1107)
                     /locus_tag="VFH91_00065"
     CDS             complement(172..1107)
                     /locus_tag="VFH91_00065"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HET6861395.1"
                     /translation="MRPKRRQEIITLLVVFLAVLVVSALIVYSIGGWRRSNADNYRPV
                     VQSQPGDANRSTAEGRSQPSAGNPQSRGIIDSIFGSQSNGETGSSEGWVINIGHMTVR
                     LSLAALLGAALAYRPRKRILALKRNPYVAQTQILLAIVAAALMIIVGDNAARAFGIFA
                     AVSLVRFRTNIRDPKEITVLLISLALGLAAGVGRWELALILAIFSLIVLWLLEWREPS
                     LVFRSMEVKVTTKNVVATQKALREVFKKFGFDRELRAVDREVSEESPGSLMYSVDISP
                     TVTTDEISEEILHLDGLNIQGIEWDQKKSYSYLYQ"
     gene            complement(1137..2108)
                     /locus_tag="VFH91_00070"
     CDS             complement(1137..2108)
                     /locus_tag="VFH91_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861396.1"
                     /translation="MRRGKFVSTIVLLAIVLSVISINTGFAKPKEKKRLPSGTAVLWR
                     SPDDIGSRDLYLGPGGSSMRPDLRRLIFLKEEKGGYSKKYKVRDASGREWVAKIGKEA
                     QSETAAIRLLWGVGYLTEVNYLAPRVTIPGKGTFTNVRFEARPKDWDRLQEWKWKQNP
                     FVHSREFQGLKVMMVLLNNWDIKDSNNRIVYVRGDDGDELRYIISDLGATFGHASTTP
                     LFWRFTRSRNNPKNFAKSDFLEKVKGDRVVLHYGGKNRGLFKDITIEDALWIASLLAD
                     LSDQQLTDAFRAANYTPNQSALLANVIRRRTNELLSLRPRLQIGQGQ"
     gene            complement(2201..4336)
                     /gene="ppk1"
                     /locus_tag="VFH91_00075"
     CDS             complement(2201..4336)
                     /gene="ppk1"
                     /locus_tag="VFH91_00075"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET6861397.1"
                     /translation="MSTVVKKQSEVRPFPKLVETVNTPVRSDMFLNRELSLLKFHARV
                     LEEAFDERNPLLERLKFLSIFTSNLDEFFMIRVSGLKLEIEEEVDVLSADNQTPEQQL
                     GRIREQVLLLTEQQSKCWLEDLQPRLAKDGLEVANYDSLTGHEKEILNNYFSEHVFPI
                     LTPLAVDPSHPFPYISPLSVNLCLIVEPPEDPPYFGALKFDEPKFVRIKVPSVLPRLI
                     PVNESNTKFVLLEEIIEANIQSLFPGMLPGPCYRFRVTRDADIEIREEEAHDLLSQIQ
                     EELRQRRFGTPVRLEVSPNMPDEMIDYLVESLKLVKEDVYQVDGPLHNQDLMLLFDQD
                     RGDLKDVPFEPVTPEWFSEHSSIFEAISERDRLLHHPYDSYDCVTEFINQAVDDDDVV
                     AIKICLYRTGPDSPIPPALIRASEKGKQVTALIELKARFDEEHNIEWARKLDHAGVHV
                     VYGILGLKTHGKLTLVMRREGEKLKKYVHIASGNYNPTTSCTYTDLGLFTVDETIGKD
                     ATELFNFLTGFSAQTDYKTLMVAPVDLREKLNALLDREIEHKRAGRPSGIVAKFNRLA
                     DQQVIEKLYEVSRAGVKIDLIVRGICMVRPEVPGLSENIRVRSVVGRFLEHSRVLYFA
                     NGGNEELYIGSADWMSRNLKHRIEVMAPVNDPEEKKFLKDVLLAAYWRDNTKARSLDA
                     EGNYNLIPSNPGQAFNSQEFFIGLTRESR"
     gene            complement(4560..>5136)
                     /locus_tag="VFH91_00080"
     CDS             complement(4560..>5136)
                     /locus_tag="VFH91_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6861398.1"
                     /translation="DDRVVAVGDLSVKGPKSREVLDLFMKDPRFSSVIGNHDYALVKH
                     FRTGVKLKSSQARVFEELRTPDDRYLRFLESLPFFLEVGSRLVVHAGLRPGVALEEQS
                     NDDLIELRTLGADRTDRTGTPWYEEYDGPSLAIFGHWPSPQPRRGKFALGIDSGCVYG
                     YQLTGYILESEEFLKVQALKEYAASPSTVRT"
BASE COUNT         1272 a         1328 c         1219 g         1317 t
ORIGIN      
        1 tcggtgcctt cagctgcgtt gaattccacc aactgccgtt ggtggaattc caagagactc
       61 tcggttgctc ggtgtgtagg ttggacttta agcgaccacc aactgccgtt ggtggaattg
      121 ctccgcttgc ccccactttg aatcaacatg aaaaaacgga aacttttgtt ctcactgata
      181 caaatacgaa taactcttct tctgatccca ctcaatcccc tgaatattca gcccatcaag
      241 gtgcagaatc tcctcgctta tctcgtcagt cgtgaccgtc ggactgatat caactgaata
      301 catcaggctg ccgggtgact cttcgctaac ctcgcgatca actgcgcgga gttcccgatc
      361 aaatccgaac ttcttgaaaa cctcccttag agccttctgt gttgcaacaa cgttcttagt
      421 cgtaaccttt acttccatcg agcgaaacac cagcgaaggc tcgcgccact ccaaaagcca
      481 aagaacaatc aaagaaaaga tggcgagaat taaagccaac tcccagcgtc caacgcccgc
      541 cgcaagacca agcgctaaac taatcagcag tactgtgatc tcctttggat ctctaatatt
      601 cgtccggaag cgaacgagcg acactgctgc aaagatgccg aacgccctgg cggcattgtc
      661 gccgacaatg atcatcagag cagccgcgac tatggcgagc agtatttgcg tttgcgcgac
      721 gtaggggttc cgttttaggg caagtattcg tttgcgtggt cggtatgcca gcgcggctcc
      781 aagtaatgcg gcaagagaca gtcgcacggt catatggcca atgttgatta cccagccttc
      841 agatgagcca gtttcgccgt tactctgcga gccgaaaatg ctgtcaatga tgcctctgct
      901 ttgtgggtta ccagcagaag gctgggaacg gccttcggct gtggatctat tcgcatctcc
      961 aggctgactc tggacaacag gtcgataatt atccgcattc gatcgtcgcc accctccgat
     1021 tgaatagaca atcaacgcac tcacaactaa aaccgccagg aacactacga gcagagtaat
     1081 gatctcctgg cggcgtttcg gtctcacggt cattcgaggt attcgccttt caaaacctac
     1141 tggccctgcc cgatttgaag tcttggccga agactcagta gttcgttggt acgccgtcga
     1201 atcacgttcg caagaagcgc gctttgatta ggcgtatagt ttgcggccct gaatgcgtcc
     1261 gttaactgct gatcgctcag atctgcaagg aggctggcaa tccataaggc gtcttcgatg
     1321 gtgatgtctt tgaagagccc gcggttctta cctccatagt gcaggacgac gcggtctcct
     1381 ttgaccttct caagaaaatc gctctttgcg aagttttttg gattatttcg acttctggtg
     1441 aatcgccaga acagaggtgt cgtgcttgcg tgcccaaacg ttgcgccgag atcactgatg
     1501 atgtaacgca actcatcgcc atcgtcgccg cgaacgtaaa caatccgatt gtttgagtct
     1561 ttgatgtccc agttattaag caggaccatc atgaccttca gaccctgaaa ttctcgacta
     1621 tgaacaaatg gattttgctt ccacttccac tcctgaagtc ggtcccaatc cttcggtctc
     1681 gcctcaaacc tgacgttagt gaatgttcct tttcccggaa tcgtcacacg cggcgccaga
     1741 tagttaactt cagtcaaata cccaacaccc caaagcaatc tgatggcagc agtttctgac
     1801 tgtgcttctt tgccgatctt cgctacccat tcgcgtccag atgcgtcgcg caccttatac
     1861 ttcttcgagt aaccgccctt ctcctctttt aagaagatga gccgccgaag atctggccgc
     1921 atactgcttc cgcctgggcc aagatacaga tcacgcgaac caatatcgtc tggactccgc
     1981 cagaggacag cggtgccgct cggaagtcgc tttttctcct ttggcttggc aaagccagtg
     2041 ttaattgaga tgacgctaag aacaatcgcg agaagaacta tagtgcttac gaatttcccc
     2101 cgtcgcatgt ctgcctcctg cttgatgtca aatccaaatg acgcgtaaac gcgttactca
     2161 aaaagggggt aatcggggaa cgacggtaaa tatgggagag ctatcgggac tcgcgagtca
     2221 gcccgataaa aaactcctga ctgttgaaag cctggccggg gtttgacgga attaaattgt
     2281 agtttccttc tgcatctaac gagcgagcct tggtgttatc tcgccagtac gcagctaaca
     2341 acacgtcctt taagaacttc ttttcttctg gatcattgac tggagccatc acttcaattc
     2401 ggtgcttcag gttccgagac atccagtcag cactcccgat ataaagttcc tcgttgccac
     2461 cattcgcaaa gtagaggacg cggctgtgtt caagaaacct tccgacgaca ctacgaacgc
     2521 gaatattttc tgagagtcca ggcacttccg gtcgcaccat acagatcccg cgcactatca
     2581 agtcaatttt tactccggcg cgagagactt cgtagagctt ttcgatgacc tgttgatccg
     2641 caagtcgatt gaattttgcc acgatgcctg aaggacgacc ggcacgtttg tgttcgattt
     2701 cgcgatcaag taaggcgttc agtttttcac gtagatctac aggtgcgacc atcaacgtct
     2761 tatagtcagt ttgggccgaa aaacccgtca gaaagttgaa cagctcggtt gcgtctttcc
     2821 caatcgtctc atctacagta aacagaccca gatccgtgta cgtgcatgaa gtggttgggt
     2881 tgtaattgcc agacgcgata tgaacatact tcttgagctt ttcgccttcg cgtctcatca
     2941 cgagcgttaa tttgccgtga gtcttaagcc caagaattcc atacacgaca tgtacgccgg
     3001 cgtggtccaa cttccttgcc cattcgatat tgtgctcttc gtcaaaacgc gccttgagct
     3061 caatcagggc agtgacttgc ttgccctttt cgctggccct gatgagtgcc ggtggtattg
     3121 gcgagtctgg tccggttcga tataaacaga tcttgatcgc cacgacatca tcgtcgtcta
     3181 ctgcctgatt gatgaattcg gttacgcagt cataagagtc atacggatga tgaagcagac
     3241 gatctcgctc agaaatcgct tcaaaaatgc tcgaatgttc gctaaaccac tctggtgtga
     3301 cgggctcgaa aggaacgtct ttcaaatcac cgcggtcctg atcgaaaagc aacattagat
     3361 cctggttatg aagtggacca tcgacctgat acacatcttc cttgaccagc tttaacgatt
     3421 cgacgaggta gtcaatcatt tcatccggca tgttcgggct gacttccagc cggacaggtg
     3481 tgccaaaccg tctttggcga agctcttcct gaatttgact cagaagatcg tgcgcctcct
     3541 cttcacggat ctcgatatcg gcatccctcg taactcgaaa gcgatagcat gggccaggta
     3601 acatgcctgg aaacagcgat tggatgttcg cctcgattat ttcctcgagc agcacaaact
     3661 tcgtgttcga ctcattaaca ggaataagcc tcggcagaac agaagggact ttgattctga
     3721 caaatttcgg ttcatcaaat ttcaatgcgc caaagtaggg tggatcctcg ggtggctcaa
     3781 caatcaaaca caggttcacg ctgagcggag atatataagg aaacggatgg cttggatcta
     3841 ctgccagtgg tgtcaaaatc ggaaatacgt gttcagaaaa ataattgttt agaatctcct
     3901 tttcgtgacc cgttaaggag tcatagttcg ctacctcgag cccgtcttta gcgagacgag
     3961 gttgaagatc ctcaagccaa catttcgact gctgctctgt taggaggagc acttgctcgc
     4021 gaatcctgcc gagttgttgt tccggcgttt ggttatccgc agataacacg tcgacctctt
     4081 cttcgatctc cagcttcagc ccggagactc gaatcatgaa aaactcatca agatttgaag
     4141 tgaaaatgga aagaaatttt aaacgctcca gtaacgggtt acgctcatcg aaggcttctt
     4201 ccaaaacacg agcgtgaaac ttaagcaggc taagctcgcg gttcaaaaac atatctgagc
     4261 gaaccggtgt gttgactgtt tcgactaatt tgggaaacgg tcgaacttct gattgtttct
     4321 taacgactgt ggacataact ttcctgggtt agctactcag ttgaagttga tagattcggt
     4381 gaactgtccg tcctcatcgt tgtccaaacg ggaacatacg agagcaatgc cgtaaatcga
     4441 gtggcaaggt gcgttccatc aacgtttacg agttaattaa gccgttttaa gttgcttatt
     4501 attaagagtg gctattgtgt tgcgaaacca atgcggagtt ttgtgtactg aaatgatgct
     4561 catgtgcgta cagtactggg agaggcagcg tactccttga gagcctgaac tttcaggaat
     4621 tcctccgact cgagaatata gccggtaagc tgatacccgt agacacagcc actgtcgatt
     4681 ccaagtgcaa acttacctcg gcgaggttgt ggtgagggcc aatggccgaa tattgccagt
     4741 gaagggccgt catattcttc ataccatggg gtgccggttc gatcggtgcg atcagcgccg
     4801 agtgtccgaa gttcaataag gtcatcgttg ctctgttctt caagcgcaac ccctggtcgt
     4861 aaaccggcat gcacaaccag acgcgatcca acttcgagaa aaaaaggcaa agactcgaga
     4921 aatcttagat aacggtcatc cggagtcctc aactcttcga aaaccctggc ttgtgagctc
     4981 ttaagtttta ctccagtgcg aaaatgtttg acgagggcgt agtcatgatt cccaattact
     5041 gaggaaaacc gcgggtcttt catgaacaaa tccagaactt cgcgactctt tgggcctttg
     5101 acactgaggt cgccaaccgc taccacccgg tcgtcg
//
LOCUS       DASSFP010000004         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1787884, whole genome shotgun sequence.
ACCESSION   DASSFP010000004 DASSFP010000000
VERSION     DASSFP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787884"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFH91_00085"
     CDS             <1..498
                     /locus_tag="VFH91_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861399.1"
                     /translation="VSEIIVSSQDGYVSQGLVTVFQGGSRILTMEDTDNGPAVAENGH
                     KQTAANFQVTTPENFGTDKRTRITFFATGISGSALNSNPNNDVNLNGVIRPNFAESVS
                     VEAQLSNGQVISLPVEFAGAQGVLPGLDQVNVVLDSALQGTGNVKLTLVVNGQRSNGP
                     SIFIR"
     gene            761..1771
                     /gene="xrt"
                     /locus_tag="VFH91_00090"
     CDS             761..1771
                     /gene="xrt"
                     /locus_tag="VFH91_00090"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HET6861400.1"
                     /translation="MSVPVPATKNVWRGILILSAVGFAYFWVLVKLSRDWWFDENYSH
                     GLLIPFIIGYILWLQRDKLAEARSAPSTLWGGLLVAGGLFGLWAGVAGAELYTQRISL
                     IILIVGVVVYFWGARILRFLIFPLVLLFLAMPIPAIVLNKIAFPLQLFASRCAVWSMR
                     MLDIPVLRQGNVIELMPLNTVHTKKLEVVEACSGIRSLMTLITLAVVFAYFTYPPRRE
                     SESGQPKQSWYRSYGFWRTFLIIGLAIPIAIITNAMRVSGTGILARYYGTEVADGFFH
                     SFSGWVVYIVAFLMLFAVGWILDRIAGMATPQKPDGEATKLERAFAKSQVKMVAESEG
                     ND"
     gene            1773..2417
                     /locus_tag="VFH91_00095"
     CDS             1773..2417
                     /locus_tag="VFH91_00095"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HET6861401.1"
                     /translation="MSRVTRFWGLFVVLIIAGALVNSWALLGEAHVERKELKNFPTQV
                     GTWTQKGVDQRLDDQTMSVLKASDYLLRDYRSTTGQFANYYVGYYASQRDGSSFHSPL
                     NCLPGSGWTMTAPGTTTVTLDNGKTFEANKYVIENEGVRQLMLYWYQGRGRVVVNEYW
                     GKVFTVLDSISMRRSDAAMVRITVPVTTSDDEALNAAKDFAKATSLSLPEFVPN"
     gene            2523..>3239
                     /locus_tag="VFH91_00100"
     CDS             2523..>3239
                     /locus_tag="VFH91_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6861402.1"
                     /translation="MTLKVLKTRKPPEQQMAEEERIEMTIECRRRSLYLPFFCLLLAA
                     LFSLAGCSNPEKAKAEHLSRGEAYLKELKFQEASLEFRNALQIDDKLAAAHWGMARSY
                     EGLERFQEMMDELRKTRELDPNNLEARVRLGNYYVVAATRNKPELWPEAQKIADEILQ
                     KDPNYIEGHILQGTIYFNQNKREEAFKELNRAIEIDPNRAESYLSLARFYIVTNDNSK
                     AEELLKKAISVNGNSPIAHTE"
BASE COUNT          806 a          739 c          848 g          846 t
ORIGIN      
        1 gtatccgaaa tcattgtgtc gtcgcaggac ggctatgtca gtcagggact cgtgacagtc
       61 ttccagggtg gttcacgaat tctgacaatg gaggataccg acaatggtcc cgcggtggcc
      121 gaaaacggac acaagcaaac agcggcaaac ttccaggtta cgacacctga gaatttcgga
      181 accgacaaga gaacccgaat aacgtttttt gcaaccggaa tcagtggaag tgcgctgaac
      241 tcaaatccga ataacgatgt gaacctaaat ggtgtcattc gtccgaactt cgctgagtct
      301 gtatcggtcg aggctcaatt aagtaacgga caagttatct cgctacccgt tgagtttgcg
      361 ggtgctcagg gtgtactgcc cggactcgac caggtaaatg tggttcttga ttctgcactc
      421 cagggtacgg gcaacgtgaa gttaacgctg gtagtgaatg gacagagaag taatggtcca
      481 agtatcttca ttcgttagta ttttctaacg atcttcgaga aagggcgatg caagttgcgt
      541 cgcccttttt cattttggcc agggcgttaa aaagtctgga aaatgaccgc gattgaaagt
      601 tttgaacaat ttgtaacaat tcgttcccca tgattgtcac tggagacgac ttgatataag
      661 ctctttcgcg cgtttaaacg ggtgaatcgc tttacctaca cccaccggtt ttcgcgaggg
      721 aaattgccct tttcttgcga aaatgttcaa ggaaatctgt atgagtgtcc cggtcccggc
      781 caccaagaat gtctggcgcg gaattctaat cctctcagca gttggctttg cctatttctg
      841 ggttctggtc aagctctctc gtgattggtg gttcgacgag aactattctc acggcctcct
      901 aattccattc atcattggat atatcctgtg gcttcaaagg gataagcttg ctgaggctcg
      961 gtctgccccg tcgactctct ggggtgggtt gttggttgcc ggcggtttat tcggactttg
     1021 ggccggagtt gccggcgccg agctgtatac gcaacgaatc tcattgataa tcctaatcgt
     1081 tggagtagtt gtttacttct ggggggcgcg catacttcgg ttcctgatct ttccgttggt
     1141 ccttctattt ctggcgatgc caattcccgc gatcgttttg aacaagatcg cgtttccgtt
     1201 gcagttgttc gcatcgagat gtgctgtttg gtccatgcgc atgttggaca tcccggtctt
     1261 gaggcaagga aacgtaattg agttgatgcc gctgaacact gtccacacca agaaactgga
     1321 ggtagtggaa gcttgtagcg gaattcgctc gttgatgacg ctcattacgt tggccgttgt
     1381 gtttgcttac tttacttatc caccccgtcg tgaatcggaa agcggacaac caaagcagag
     1441 ctggtatcgc agttatggtt tttggcggac atttctgatt atcgggctgg ccattcccat
     1501 cgcaatcatt acgaacgcga tgcgcgtaag tgggacaggt atcctcgccc gctattacgg
     1561 cacagaagtc gctgatggtt ttttccatag cttctctggt tgggttgtat acatagttgc
     1621 gttcttgatg ctgtttgcgg ttggctggat cctggaccgc atcgccggga tggcaactcc
     1681 gcagaaacct gatggggaag caacaaagtt ggaaagggcc tttgcgaaat ctcaagtaaa
     1741 aatggttgca gaatccgagg gaaatgattg atatgagccg cgtaaccaga ttttggggtt
     1801 tattcgtggt cctgattatt gcaggtgccc tggtaaattc gtgggccctg cttggagagg
     1861 cgcatgttga acgcaaagag cttaaaaact ttccgacaca ggttggcacc tggacacaaa
     1921 agggcgtcga tcagcgtctc gatgatcaga caatgagtgt attaaaagcc tccgattatc
     1981 ttttgcgcga ttaccgcagc acgaccggac aatttgccaa ctattatgtt ggctattatg
     2041 ccagccaacg agatggctca agctttcata gtccattgaa ctgtttgccg ggatccggct
     2101 ggacgatgac tgctccaggg acgacaacgg tgacgctcga taacggaaag accttcgaag
     2161 cgaataagta cgtcatcgaa aacgagggtg tccggcagct tatgctttac tggtaccagg
     2221 gacgaggtag ggttgttgtc aatgagtact ggggaaaagt tttcactgtg ttggatagta
     2281 ttagtatgcg tcgttcggat gcggcaatgg tccggataac tgttcccgta acaacctccg
     2341 acgacgaggc tttgaacgct gcgaaagatt ttgcaaaagc aacatcgctg tcgttgccgg
     2401 agtttgtacc caactagact ttagggactg tcaaacactt aacacgactt tggaattaga
     2461 accagattaa gattatttac tattcgtctc agatcctgag aagtcaaagg cattgcccgc
     2521 cggtgacgtt gaaggttctg aagacgcgaa agccacctga acaacagatg gctgaagagg
     2581 agcgaattga aatgacaatc gagtgccgtc ggagatctct ttacctaccc tttttctgcc
     2641 ttcttttagc tgccttattt tccttagcgg gctgctcaaa tcccgaaaaa gccaaagctg
     2701 aacatttaag tagaggcgaa gcttacctga aagagttgaa gtttcaggag gcctctctcg
     2761 agtttcgtaa cgctctacaa atcgacgaca agctagctgc cgctcactgg ggcatggccc
     2821 gctcttacga aggacttgag cgtttccagg agatgatgga cgagcttcgg aagacacgag
     2881 agctcgaccc gaacaatctc gaagcgcgcg tcaggctcgg caactattac gtggttgctg
     2941 cgacgagaaa taaacctgag ttgtggcccg aggcgcagaa gatcgccgac gaaatacttc
     3001 aaaaagaccc aaattacatc gaaggtcata ttcttcaggg caccatttac tttaatcaga
     3061 acaaacgaga agaggcattt aaagagttaa atcgtgccat cgaaatcgat cctaatcgtg
     3121 cggagtcgta cctgagtctc gctcgctttt atattgtcac aaatgacaat tcaaaggccg
     3181 aagagctgct taagaaagca atttcggtaa acggcaactc gccaattgca cacaccgag
//
LOCUS       DASSFP010000005         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_150056, whole genome shotgun sequence.
ACCESSION   DASSFP010000005 DASSFP010000000
VERSION     DASSFP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150056"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFH91_00105"
     CDS             <1..1080
                     /locus_tag="VFH91_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6861403.1"
                     /translation="VLEIIGESQTEQRLRALLNIAIVEKAATRDSEALRIYREAATLF
                     DLSSNHALKGKFHNSYANLLRTIGLSENKDEFVDKALVEFAAASFHFEQAGHKRNQAR
                     VENNVGYLFATIGRFPDAHDHLKHALSLMKALKDQGGTAMVEDARAQAFLMEGKNADA
                     ERTAREAVKVLSGGGEQVVLAEALTTYGKALARIGRFNESKINLDKATEIAQVAGDPN
                     RGGIAAVTAIEELSKNLSAGELKNYYRSAEELISNSQNRALKIRLGECARLVLAADAN
                     VDQAKSASQSFSPDFSLESEVLRYEGNLIRQALETSGGSVTRAARLLGVTHQGLAFIL
                     NGRHSDLLSVRTPVKKRRRSIIRYH"
     gene            1225..2790
                     /locus_tag="VFH91_00110"
     CDS             1225..2790
                     /locus_tag="VFH91_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6861404.1"
                     /translation="MTLVKSCVVVLLVLTTILTTIEPSNAQTKRTPTRRTATTTATST
                     QPAQPVAPPATPAVSTIPAVPVQLAVVNGQTISSSDLDPSVRQELESTEARIAGARRS
                     ILDVQINTILLEVEARRRRISTHRLYELEVVRRIPTPTSAQIKQFLDDNRAQFEGIDP
                     AEAAPQAEALMHDQFESRLADDLVGRLRKAIPVVMGPDINTPNLAPQAVVATIGGQPL
                     TGAVINERLKPIIYKIQFTAYQAGKVQADQTIDDILLLAEANRRQITPEEMVRKEISE
                     KVKSPTDAEVDKFYNENKSRINGDLSTVRNQVVNYLQTEDRTRLEKELSARLRKGADI
                     RWLISEPVAPVLNISVDDDPSRGDANAPVTLVEFTDFQCPACAAMQPVLEEVLQSYGN
                     KVRFVVRDFPLAQHENARKAAEAANAANAQGKFFEYTTLLFKRQKALDVASLKKYASE
                     IGLNRAKFDAALDSGTYQEEVKHDIEDGEMYGVGSTPTIFINGVQLTVLSADGLREAL
                     DRALGQKKPASTN"
     gene            2944..>4470
                     /locus_tag="VFH91_00115"
     CDS             2944..>4470
                     /locus_tag="VFH91_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET6861405.1"
                     /translation="MVVSLKPDKLKRYKDVVRLFVKYGRSDLISPAGLVGSEIADEID
                     FESKAASAEDLANDLEKLGPTFIKLGQLLSTRADLLPGPYLDALSRLQDKIEPFPYDE
                     VERIVSSELGVRISKAFADFEPVPLAAASLSQVHLAYMRDGRAVVVKVQRPDIRELIV
                     DDLEALNEVAHFLDAHTELGQRYEFENMLINLRQGLLRELDFTIEANNLTTIGRNLAE
                     FVDIVVPQPVDDFTTSRVLTMDYLSGTKVTDLTPLRLLEIDGPSLAEDIFSSYLKQIL
                     VDGLFHADPHPGNILLTKENQIALLDLGMVGRVTMSFQDKFLRLLLASSDGRGELAAE
                     CAIRMGEPKPKFDRAEYFRRVADLVADNRDATMSKLNAGQIVLEITKISADCWVRMPP
                     EFTLIAKALLNLDRVVYTLDPAFEPNAIIRERATEILEQNILQSVSPSNLLSSVVDFK
                     EFAEKFPTRVNRILDSISNNELRFKVDAIDERVVIDGLQKVANRITLGLVVAALIVGA
                     A"
BASE COUNT         1163 a         1097 c         1154 g         1056 t
ORIGIN      
        1 gttttagaga tcatcggaga gtcccaaact gagcaaagac tccgtgcctt gctcaacatc
       61 gccatcgtcg aaaaggccgc gacaagagac agcgaggcgc tgcgaatcta cagagaagca
      121 gcaaccttat tcgatctcag ctctaaccat gcgttgaagg gtaagtttca caactcgtat
      181 gcgaacttac tcaggaccat tgggctgtcc gaaaacaaag atgagtttgt cgacaaggca
      241 ctcgttgagt tcgccgcggc tagttttcac ttcgagcagg ccggtcataa gcgcaatcaa
      301 gctcgagtcg aaaacaacgt tggttatctc tttgctacca tcggcagatt tccagatgct
      361 cacgatcatc taaaacatgc actctccttg atgaaagctc ttaaagatca gggtggcact
      421 gcgatggtgg aagatgctcg tgcccaggcg ttcttgatgg aaggaaaaaa cgctgacgcc
      481 gaaagaaccg ctcgcgaagc tgtgaaagtt ctttccggtg gaggcgaaca agtcgtcctg
      541 gcagaagctt tgacgacgta tggaaaagct ctcgcccgca taggcaggtt taacgaatcg
      601 aagatcaatc tcgataaggc aactgagata gcgcaggtgg caggtgatcc taatcgaggt
      661 ggtatcgcag ctgtcacagc aattgaagag ttgagcaaaa atctctcggc cggggaacta
      721 aaaaattact atcgttctgc cgaggaattg atttcaaact cacagaatcg cgcattgaag
      781 attcgcttag gagagtgtgc gcggttggtg cttgctgccg atgcgaatgt cgatcaagcg
      841 aagtctgctt ctcaatcctt ttctccagat ttttcacttg agtcagaggt ccttcggtac
      901 gagggaaatt tgattcgaca ggcactggaa acttctggcg gaagtgttac cagagcagct
      961 agattgcttg gtgtcaccca tcaaggattg gccttcatcc taaatgggcg acatagcgat
     1021 ttactttccg tacgaacacc cgttaagaag cgccggagaa gtattattcg ttaccactaa
     1081 agccggcttc taacaattcg tctcgtagac ttttggcgct gtctctccca caaggcagcg
     1141 ccaaaggctt gtttgtgctc taatcaattg ttcttttatt atgctgttcc ccaatctacc
     1201 ccgtttcctg tgagttgaac cgttatgacg ttagtaaaaa gctgcgtagt tgtgcttcta
     1261 gttctgacga caattctgac gacaattgag ccgtcgaatg ctcaaacaaa acgcaccccg
     1321 actcgaagaa ccgcaactac aactgccacc agcactcagc cggctcagcc agttgctcct
     1381 ccagctactc ctgccgtgtc cacgatacct gctgtcccag ttcaactcgc agtagttaac
     1441 ggacagacca tcagtagctc cgaccttgac ccttctgttc gacaggaatt ggaatccacc
     1501 gaggcaagaa tcgccggggc cagacggagc atcctcgacg tgcaaatcaa cacaatactt
     1561 ctcgaagtgg aagcgcgccg cagacgaatc tcgacacacc gcctctacga gcttgaagtg
     1621 gtccgccgga ttccgacacc aacgtcagca cagataaagc aatttcttga tgataatcgc
     1681 gcgcagtttg aagggattga tccggcagaa gcagcacctc aagctgaggc gctaatgcac
     1741 gatcagtttg aaagtaggct tgcagatgac ctggtcggcc gtttgcgaaa agccattccg
     1801 gtggtgatgg gaccagacat caacacgccg aacctggcgc ctcaagcggt agtcgcaact
     1861 atcggtggcc aaccgttgac gggtgcagtg atcaacgaac ggttgaagcc gattatctac
     1921 aagattcagt ttaccgctta ccaggcaggc aaagtgcaag cagatcaaac cattgacgac
     1981 atactgcttt tagcggaagc caatcgcagg caaatcacgc ccgaggagat ggtcagaaaa
     2041 gagatcagcg aaaaagtaaa atccccgact gatgctgaag ttgacaagtt ctacaacgag
     2101 aacaaatcgc ggataaatgg agatctaagt actgttcgca atcaggttgt taactatcta
     2161 cagactgagg atcgcacccg gctcgagaaa gagctttcgg ccagattgag aaagggagcc
     2221 gatattcgct ggcttatttc tgagcccgtg gcgccggtcc taaatatcag tgttgatgac
     2281 gatccgtcgc gaggagacgc aaatgcgccg gtgacactcg tggagtttac tgactttcaa
     2341 tgcccggcct gtgccgcgat gcaaccggtt ttagaagagg tgctgcaatc atacgggaat
     2401 aaagtgagat tcgtggtcag agattttccg cttgctcagc acgagaatgc acgtaaggcg
     2461 gcggaagcgg caaatgctgc caacgcgcag ggaaaattct ttgaatacac gactttactg
     2521 tttaagcgac agaaagctct cgacgtagcg tctctgaaaa aatatgcctc cgaaatcggc
     2581 ttgaataggg cgaagtttga cgcagctctc gacagcggca catatcagga agaagttaag
     2641 cacgacattg aagacggcga aatgtacggt gttggcagca ctccgacaat ttttatcaac
     2701 ggagtgcaac tgacggtgct gagtgcggat ggcctacgag aagccctcga tcgagctctg
     2761 ggacagaaaa agccggcttc aaccaattga gtttaaaaga ccagttgcaa cctggtgggc
     2821 caataagcga ttattggcac accctattcc agttttcccc ggcgatctag taatcgcagc
     2881 cgttacactc cacccctgac gaattaatcc ctcgataata ggctcccccc ggcggaggat
     2941 aggatggtag tttctctaaa acccgacaaa cttaaacgtt ataaagacgt tgtccgactc
     3001 tttgtaaagt acggccgttc tgacttgatc agtcctgctg gtcttgtggg gagtgaaatt
     3061 gcggacgaaa ttgattttga gtcaaaagca gcttcggcgg aggatttggc caacgacctg
     3121 gagaaactgg gacccacgtt tatcaagttg ggccagctcc tctcgaccag ggccgacctg
     3181 ttgccaggcc cttacctcga cgcactttca cgactacagg ataagataga gccatttcct
     3241 tacgacgagg ttgagcggat tgtctcttca gagctaggcg ttcgaatctc aaaagccttt
     3301 gcagacttcg agcctgttcc tcttgccgcg gcttctctgt ctcaggttca tttagcgtac
     3361 atgagagatg gacgggcggt cgtcgttaaa gttcagcgtc ctgatattcg agagctcatc
     3421 gtcgacgatc tggaagcgtt gaacgaggtg gcccactttc tggacgcgca taccgaactg
     3481 ggacaacgtt acgaatttga gaatatgctc atcaacttgc gacagggctt gttgcgcgag
     3541 cttgatttca caattgaagc gaacaatctg acaacgatcg gtcgaaacct ggctgagttc
     3601 gttgacattg tggtccccca acctgtcgat gactttacta ccagtagagt cctgacaatg
     3661 gattatctct cgggcacgaa ggtcacagac cttacgccct tacgattact tgaaatcgat
     3721 gggccttcgc ttgccgagga catcttcagt tcctatctca aacaaatcct ggtagatggt
     3781 cttttccatg cagatccaca cccgggaaat atcttgctga caaaggagaa tcaaatagcg
     3841 cttctggatc ttgggatggt tggccgagtg accatgtctt ttcaagacaa gtttttacga
     3901 ttgctactgg catccagtga tggccggggc gaattggcgg ctgaatgcgc aattcgaatg
     3961 ggagaaccga agccaaagtt tgatcgagca gaatacttca ggcgtgttgc tgatctggtg
     4021 gccgataatc gggacgccac gatgagtaaa ctgaacgccg gccagattgt ccttgagatt
     4081 acgaagattt cggccgattg ctgggtcagg atgccgcccg agttcacgct gatcgcgaaa
     4141 gcgctgctca atcttgatcg cgtggtctac acactcgatc cagcttttga gccgaatgca
     4201 atcattcgcg agcgtgctac tgaaatcctc gaacagaaca ttctccagag tgtttctcca
     4261 agcaacctac tgtcaagtgt cgtggacttc aaagagtttg ccgagaagtt tcctacgagg
     4321 gtcaatcgaa ttcttgattc tatcagcaac aatgagctga gattcaaggt agatgcaatc
     4381 gatgagcggg ttgtgatcga tggtttgcag aaggtcgcga atcgaattac gttaggactg
     4441 gttgtagccg cgttgattgt gggtgcggct
//
LOCUS       DASSFP010000006         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_745840, whole genome shotgun sequence.
ACCESSION   DASSFP010000006 DASSFP010000000
VERSION     DASSFP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745840"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFH91_00120"
     CDS             <1..739
                     /locus_tag="VFH91_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6861406.1"
                     /translation="ISVADPLVPAKKLVDNVSFSVRRGEVLGIAGLMGAGRSDLLMAI
                     FGAHSGAVSGDVLIEGKPTKIGSPAAAIKNGIGFVTEDRKRFGLVLDQTILNNMTLAG
                     LPKISGRFITSVDAEAAASERSMKELRVKSNSVFTIAGTLSGGNQQKVVLAKWLLTNP
                     RVLFLDEPTRGIDVGAKQEIYAQINKLAKSGLAIVLVSSELPEVLGLSDRVIVLHEGR
                     VTGEFTRSTASPERVMACATGQMKRTA"
     gene            816..2066
                     /locus_tag="VFH91_00125"
     CDS             816..2066
                     /locus_tag="VFH91_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6861407.1"
                     /translation="MTNTNEAEVDRPTANADLVPIAPPAEITPQTTIGGWKVPTELLR
                     AYTMVFALIAIWIYFHYSTDHIFLEPRNFSNLMRQTAVTGVLAVGMLMVIITGQIDLS
                     VGSVVGLAGGVAAIAQGWLGWGLVPSLAAGIAVGLTIGAVQGTLTSYVNIPAFIVTLG
                     GLLAWRGVILGLSRGETIPIRLQSFKSLGQDYVGPTVGWLIAAFAVAAIVWSTINRNR
                     ARRRFGLAVPHTLTTFARIVVPALVVVGFIYLMNSYQLAPGTYAGIPVPVIIFLTIAL
                     AGAFVTQNTTFGRYLYAIGGNPDAARLSGINIRRHLLAVFCIMGALTGVASIIYTARV
                     GSASPDAGQLLELDAIAACVIGGTSLMGGRGTVFGAILGALIMASLDNGMSLKNVEPY
                     MQNIIKGAILVVAVGLDMAGRKRG"
     gene            complement(2349..2525)
                     /locus_tag="VFH91_00130"
     CDS             complement(2349..2525)
                     /locus_tag="VFH91_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861408.1"
                     /translation="MSKTKEQKSDDNKRRVQVGDLQQQERELKSHEAENVKGGGGAAG
                     GVVMRTTIGEEIPS"
BASE COUNT          660 a          708 c          775 g          751 t
ORIGIN      
        1 catctccgtg gcagacccgc tcgttccggc gaagaagctt gttgataacg tgagtttctc
       61 ggttaggcgc ggagaagttc tgggtatcgc gggcttaatg ggcgctgggc gaagcgatct
      121 tctcatggca atttttggcg cacactccgg cgcagtctca ggagacgtcc tgatcgaagg
      181 caagccaacg aaaattggga gtcccgcagc agcgatcaag aacggcattg gctttgtcac
      241 agaagaccgt aagcggtttg ggctagtact tgaccaaaca attctcaaca acatgacgct
      301 cgcgggatta ccaaaaattt ccggacgctt catcacgagc gtcgacgctg aagccgccgc
      361 tagtgaacgt tcaatgaagg aattgcgcgt caagtcaaac tccgttttta cgattgccgg
      421 tactctctcc ggaggcaatc aacaaaaggt ggtcctcgca aagtggctgc tcacaaaccc
      481 ccgagtgttg tttttggatg agccaactcg gggcatcgat gtgggagcaa aacaagagat
      541 ctatgcacag attaataaac tggcaaagag cggtttagcg attgtgttgg tctcatcgga
      601 attgcctgaa gtgctcggtc tttctgatcg agtaatcgtg ttgcacgaag gacgggtcac
      661 aggtgagttt actcgttcaa ctgcgagtcc tgagcgcgtg atggcatgcg cgacaggaca
      721 gatgaagcgc acagcgtaga gagaagccgc cgtcatctca atgaagtcta gaagatcaat
      781 ggatagcagg agaccggccg atagggaaaa cttggatgac caataccaat gaggccgaag
      841 ttgatcggcc aacagctaac gcggatctag ttccaatagc accgcccgca gaaattactc
      901 ctcaaacaac catcggaggt tggaaagttc cgaccgaact tctgcgcgcc tacacaatgg
      961 tttttgcgct cattgcgatc tggatctact tccattactc aaccgatcac atatttctcg
     1021 agccgcgtaa tttttcaaac ctgatgaggc aaaccgccgt tacaggtgtg ctggcagttg
     1081 gaatgttgat ggtgatcatt acgggccaga tcgatctatc tgtgggctcg gtggttgggc
     1141 tggctggtgg agttgccgcc attgcgcagg gctggctggg ctggggcctc gttccgtcat
     1201 tggctgcggg aattgcggta gggctaacca taggtgctgt tcagggtact ttgacatctt
     1261 atgtcaacat tcccgcattc atcgttactc tcggtggtct gcttgcctgg cgcggcgtga
     1321 ttctcggtct cagtcgtgga gaaacgattc cgatacggct gcaatcattc aagtcacttg
     1381 ggcaggacta tgttggacca acagttggat ggctcatcgc cgcgtttgcg gtggctgcaa
     1441 tcgtatggtc gaccataaat cgcaatcgtg cgcgacgtcg ctttgggctg gccgtcccac
     1501 ataccttgac gacgtttgcc cgtattgtag tgccggctct cgttgtcgta ggttttatct
     1561 acttgatgaa ctcttaccaa ctggccccgg gtacatatgc aggtattcca gttccagtaa
     1621 ttatctttct cacgattgct ttggcaggtg catttgtaac tcagaacaca accttcggtc
     1681 gttacctcta tgctattggt ggcaatcccg acgcggcccg tctatcaggc ataaacattc
     1741 ggcggcacct gcttgcggtc ttctgcataa tgggcgctct tacaggtgtg gcctcgataa
     1801 tctacacagc tcgtgttggc agtgcttcgc ctgatgccgg tcaactgctc gaacttgatg
     1861 ctattgcggc atgcgtgatt ggcggtacga gtttgatggg tggacgaggc accgtcttcg
     1921 gagcgattct cggcgccttg attatggcaa gcctcgacaa tgggatgtca cttaaaaatg
     1981 ttgagcccta catgcagaac attatcaagg gagctattct tgttgtagct gtaggattgg
     2041 atatggcggg ccgcaagcga ggctgatgcc gtgggcttgt cctgcccgag cacgtaatga
     2101 ataagttcaa agcgatctgc gagatggtct aactctcggt cagaaagata ctgaatgctg
     2161 tagtccctct cagcagttct ttagttcggt cgaattcatc ggttgcctcc tgccgaacga
     2221 gagctttacc gaatcttctc gtacagttgt ttaagttcca tcgggcggta agccgtccga
     2281 tggaattagc ggtcaacgag ctagagcact ggcttcgatt ttttgtgaca agccaggcag
     2341 ctcgcaaact aactagggat ttcctcgcct atagtggttc gcatcacaac accacctgca
     2401 gcaccgcccc cacccttaac gttctccgct tcgtgacttt tcagttccct ttcctgctgc
     2461 tgtaagtcgc caacctggac acggcgcttg ttgtcgtccg atttttgttc tttagttttg
     2521 ctcataatcg ttttccttca tttgtttctg tctgtcgatg gtctgtttgc ctcttaagca
     2581 aagtggcctc ccaatctata agtgaacaca aagggcaaaa catcactgcg atgtccgata
     2641 tgctttgtta gcttgtcgtt aggtgagcca gcagaacgtg atgtccgata tgctttaagc
     2701 ttgtcggtaa atgagccaac agaacctcta gagctacttg gtgcggaatc ccccgacaag
     2761 ctaaagcata tcggacatcg tagtgatgtt tttgggccag ttgtcactta tcagacggag
     2821 ggaagagaca ttcccttaga ccaggaaact tgaacatcag aagaaaggaa aatgtcgatg
     2881 agtgaaaaga agaa
//
LOCUS       DASSFP010000007         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_746016, whole genome shotgun sequence.
ACCESSION   DASSFP010000007 DASSFP010000000
VERSION     DASSFP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746016"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFH91_00135"
     CDS             <1..597
                     /locus_tag="VFH91_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6861409.1"
                     /translation="IGDKANDLILSIYEQVERENGPRDRRFRIEHAQHLRSIDIPRFK
                     KDQVIASMQPYHAIDDGRWAEKRIGPARAKTTYAFRSLLDNGARLAFGTDWTVAPLNP
                     MLTIYAAVTRRTLDGKNPNGWIPEQKISVEEAVQAYTVGSAYAEFQEAVKGSLAAGKL
                     ADFIIVDRDIFKTKPEELEKGIVVMTIVDGRVVYEREE"
     gene            complement(579..1871)
                     /locus_tag="VFH91_00140"
     CDS             complement(579..1871)
                     /locus_tag="VFH91_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6861410.1"
                     /translation="MQNSTNEGLVRGIRRWDLVAITINGIIGAGIFGLPSKVYALIGT
                     YSLLAFAACTIVVVLIILCFAEVSSRFEDTGGPYIYAQSALGPTVAFEVGWLIWLARL
                     TAFGANCNLLVNYLTYFWPSANQPGFRTTIIVGVVMVLTVINLIGIRQAAMVSNVFTV
                     GKLIPILIFIATGLFFLNPAAFEFGPAPSNGPFSQSVLLLVYAFTGFEMATIPAGEVR
                     NPRKNLPHALLIAIAVVGFLYILIQIVCVGTLPELAQSQKPLADAGVRFLGVAGGAII
                     SAGAVISITGNLNVLVLSGSRVVFAMAEQKQLPSLLARVHPSFSTPYIAIVITATVML
                     VLTLSSSFVQALTISTIARLVTYGTTCLSLPILRRRSDNEPATFKLPFGNVIAGLSLL
                     IIVWLLAHSTLQEAKVATVAAAVGLLIYLVHRVYSSRS"
BASE COUNT          712 a          606 c          625 g          538 t
ORIGIN      
        1 atcggcgata aagccaatga tctgattctc tctatttacg aacaggtcga gcgcgagaat
       61 ggcccacgcg atcgtcgatt caggatcgaa cacgctcagc acttgagatc aatcgatatt
      121 ccgaggttta agaaggacca ggttattgca tcgatgcagc cttatcatgc gattgacgac
      181 ggtcgttggg cagaaaaacg aataggacca gcaagagcca aaaccactta cgcctttcgc
      241 agtctgttgg acaatggtgc acgcctggca ttcggcactg attggaccgt cgcgccattg
      301 aatccgatgt tgacaattta cgctgcagta accagacgaa ctctcgacgg caagaatccg
      361 aatggctgga tacccgaaca aaagatttct gtcgaagaag cggttcaagc ctacaccgtt
      421 gggtcggctt acgccgaatt tcaggaagcg gtgaagggaa gtcttgcggc gggaaaactc
      481 gcggacttca taatagttga tcgggacatc ttcaagacaa aacctgagga actcgagaaa
      541 ggaatagttg tgatgacgat cgtcgatggc cgagtcgttt atgagcgtga agaatagacg
      601 cgatgaacca ggtaaataag cagcccgacg gccgcggcaa cggtcgcaac tttggcttcc
      661 tgtaaggtgc tgtgagccag cagccacacg attatcaaaa gcgatagtcc agcgatgacg
      721 ttgccgaagg gtagtttgaa agttgcaggc tcgttgtcgc tgcgtctacg taatatcggc
      781 aatgacagac acgtcgttcc gtaagtaacg agtcgggcaa tcgtactaat ggtcaacgcc
      841 tggacaaacg aactgctaag agtgaggaca agcatcactg ttgcggtaat aacgatggct
      901 atgtagggcg tcgagaagga agggtgaact cttgctaata aagatggcaa ctgtttttgc
      961 tccgccatcg cgaacaccac tcgtgaaccc gacaaaacga gaacgttcag gttaccggtg
     1021 atagagataa ctgccccggc tgaaataatt gctccgccag ccacaccaag aaaacggacc
     1081 cccgcatcgg ccaacggttt ttgagattgt gcgagctcgg gtaacgttcc gacgcaaacg
     1141 atttgtatca ggatgtacaa gaaaccgacg accgcaattg caattaagag ggcatgaggt
     1201 aagtttttcc tgggattcct tacctctccg gcaggaatag tcgccatctc aaatccagtg
     1261 aatgcgtaaa ccagcagcaa gactgattgc gaaaacggac cattcgatgg ggccggtcca
     1321 aattcaaaag cagcgggatt cagaaagaac aggcctgtag cgataaagat caaaataggt
     1381 atcagcttgc ctacagtgaa tacgttactc accattgccg cctgccggat gccgatcaaa
     1441 ttgattacgg tcaggaccat tacaactccg acaattatcg tggtccgaaa cccgggttga
     1501 ttggcggaag gccagaagta agtgaggtag ttcacgagca gattgcaatt ggctccaaaa
     1561 gcagttagtc gagccagcca gatcaaccaa ccaacctcaa aagctacagt tggaccaaga
     1621 gccgattggg cgtagatata tggtccaccc gtgtcctcga acctactgct gacttcggcg
     1681 aaacaaagaa tgatcaggac gactacaatc gtgcacgcag cgaatgcgag caaactgtaa
     1741 gttccgatta gcgcgtagac cttggagggg agaccaaata ttccagctcc aattatgccg
     1801 ttaatggtta tggctacgag gtcccagcga cgaatgccac gaacgagccc ttcgttggtt
     1861 gagttttgca cgctgcctta tagcaaatta acgtcaatgg agtcatctat ttagattgaa
     1921 cagtactggt gcagtttggg atgtccgata tgctttagct tgtcgtcttc tgtgcaataa
     1981 ggttcattta gaatcgaatc ggcccaaacg cgaacacgac aagctaaagc atatcggaca
     2041 tcccaaactg catcactact gattgaacag tacttctcgg cgcctccgcc tttgcgttgc
     2101 acatcctgtc actgcagagc cgcaaagaga cagagaaagc gcaaaggtca aatgcattca
     2161 agaatcgagt tgaaattcag ccaacacgcg actaaagtaa ccagaattgc acagcgttac
     2221 ctcaggagca cgcattctcc atatcaggta gtgtcctaac ttggcttact ctgtgcaacc
     2281 tttgtgttct ctgtgcctct gtggtgagca aggtcctaag taaaccatca ccacagagcc
     2341 acaaagatct cagaggcagc acagagaagg tccggattag gacaccacgc caaatcatga
     2401 ctcagacaaa gttaccgtga gatcaacaat caaatccgcc accatactat tcgccatcgg
     2461 actgtttgtg cctctcgtca g
//
LOCUS       DASSFP010000008         8852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1192639, whole genome shotgun sequence.
ACCESSION   DASSFP010000008 DASSFP010000000
VERSION     DASSFP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192639"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFH91_00145"
     CDS             complement(<1..332)
                     /locus_tag="VFH91_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267462295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861411.1"
                     /translation="MKRNWLLILGVALVVVAVVLIFNSSGHEQAKVSAPPSHSHSPAE
                     QPLERNAIPAHFEEAPARASLGPTLPPEQFFGKAKEAYIVVREIPQTIAQLPCYCHCD
                     RGFGHKSL"
     gene            complement(495..965)
                     /locus_tag="VFH91_00150"
     CDS             complement(495..965)
                     /locus_tag="VFH91_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861412.1"
                     /translation="MKIRTSTTFILMGLLLVVFTAVGLAQTQSSQNQKQTKDASTMDS
                     CCCAAHQKDATSVAKTDGQKSGCCCDESCDMNAENKGMKHHAASSGCCCCDGDSCDMT
                     KMDAKANHSSGHECCCKGDSCDMKEGSQMSQSAGASCCNMKMKHDSMKSEKPKQ"
     gene            complement(1065..1553)
                     /locus_tag="VFH91_00155"
     CDS             complement(1065..1553)
                     /locus_tag="VFH91_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861413.1"
                     /translation="MKAKLGLMDLLRPIRLVRLLLATCVSLWLAGAGCLFGCSYGSGA
                     VVQARASLNSVVAGTSCHSMHAHDCCAKKQPSKLDKAAPQLQKLSVNAVPSGMMSDCP
                     LAVNQSAAVSPVKSDATAVLSLHSPVPGFDFANISPRAASAPLSIPNRGPTHLLHCVF
                     LI"
     gene            1701..1919
                     /locus_tag="VFH91_00160"
     CDS             1701..1919
                     /locus_tag="VFH91_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861414.1"
                     /translation="MGIDKNKIKTQDDLNEPAPMDQIEARVDAKMKRLEGKAKQNVGE
                     GLSDNSLAREGEKLRKEGEKALNKTKNS"
     gene            complement(2011..2655)
                     /locus_tag="VFH91_00165"
     CDS             complement(2011..2655)
                     /locus_tag="VFH91_00165"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6861415.1"
                     /translation="MSSKESKAPINGFWSPLRIVATIIVLGLFAAFGVSSCNSNDPSP
                     VAGPTNSAPANAPQQTASPGLSALPPPVLQAQMRSANGAQIKLSDYSGKVMLVNLWAT
                     WCGPCRIETPELVRLHKEFQSRGVEVVGLSTEDPDASAQSVRQFVKDNNVDYHIGWAT
                     PEVAAILMQGRGSIPQSFIITRDGHILKRFIGFHPQLTPPQIREALEQALNYTG"
     gene            complement(2709..3980)
                     /locus_tag="VFH91_00170"
     CDS             complement(2709..3980)
                     /locus_tag="VFH91_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861416.1"
                     /translation="MKSCPKCNRTFPDEGQKFCTFDGGLLIAPQTFDPNATIRATSAD
                     LTPASEKSTSRELTDPNATIMESYPATIALPRNTTGPTSSPKNTADIAPPQPQPPATP
                     PAAPPPPPPPLAATIATTVPPPPPPPPILESPVAQSPSAPLPSAPAARPPAAPAKKSK
                     LPLIIAGVLLLLLLGGGALAGVFFFVVKPRLDQMAGNKVPEPTPVPANTNTTANVNAQ
                     PSPTEQPSPSPEDNFVPPPNTVEFANSKDKLDGRLAEHYFDFSFYYPKNWQSDPKAGV
                     GGSSNFVKVERSIPPDFTQENFAVGWYTSSGTFIGDLPTYPKRVEELSAALAKNIPEY
                     KKISEGPTKVNSMQGYEFRWEGFSRGTEKGDLHLWGRVIFLPTGKEGDTAGATLTMLT
                     TSLAPELSSIEDVGDKGEAPVILDSFRFGKK"
     gene            complement(4021..5262)
                     /locus_tag="VFH91_00175"
     CDS             complement(4021..5262)
                     /locus_tag="VFH91_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6861417.1"
                     /translation="MRANATLRMLVLSAACFSVCLLAASAVLAQDVGADVGGGAGIFR
                     AKNPETKTKSKSPATPTKPSNRPATSRPKAPAVEERVEELLEKGNGFRDARKYAEAEN
                     AYQSILKIKPKDARAAYGLGNVYSDQQKWDDAETAYRNAIQWSPNNADAYLALSVVLV
                     QPKPGGDNAKRFADSEMAARRSVQIDPQNAVGWDRLGVALQARGIVNSETEHCYRRAI
                     ELDPQFAVAYAHLARVLNRTNRQAEAVPLYAKATELAKDPATLNLIAESLQAEQLWPS
                     SEPVLKQALSLDGRNPTSLILMARLLIVYKRFSEAEPYLKTATEVSPRGYQPYNLLGR
                     VYLAMDRFDDAATTYERASEFAAPGERKQLAGIYGFEGVGDGYMKAKQKANAAKAYQR
                     ALELDPGNGGISQKLATARAN"
     gene            complement(5460..7808)
                     /locus_tag="VFH91_00180"
     CDS             complement(5460..7808)
                     /locus_tag="VFH91_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6861418.1"
                     /translation="MKECPVCKRCFPDEINHCPQDGDVTASTLVGEPTLDGRYVLERR
                     LGQGGMGVVFQARHIFLKTSHAIKVILPDLVGNDPMLATRFRQEALAAAAIRHQNIIA
                     VTDFGVVRGTMPFLVMEFIKGRSLQDVLGQEGAMSPERAWELISPVAAGVGAAHRQKI
                     VHRDLKPLNIMLQDEVPVAEGIKILDFGLAKIKSGELLGSFVQAQTSGLMGSPFYMAP
                     EQWSDDEPDARADIYSLGVILYQMLCGEVPFKGSSIPSIMKKHLTLPPPSFQSMGVSV
                     PPAVEGVVRHALEKEADARIESVGTFLKELHAALNSSPGPASKPRQTGGLDPNQTIAS
                     AFSSTTNQADPLLATGSQSPDTNDTQFAASFESLAGTVSASELDTNVKQQLSDTAAAH
                     RREKEEQERFAREEQKRLEGKKAEEERRKLEEAAKKEREAREREQQEKFERMAVQAKE
                     LEDRLERLSVSVSPAAAIDPETTHVQRVATTTQAITGGIPVEAGHVLPESGTKKAVPV
                     LLVAAGIVILVLAGIGIGGYLMFRPKPAAVVTTQPPPSTDKPTTVKAELLAIDGGKFM
                     MGRDRGPRQETPGHPIEVRGFLMDRTEVTNAEYADFVRETNHAYPSHWGSPKPPFGQE
                     KWPVVNVSFDDVNAFAGWRSKRDSLTYRLPTEEEWEYAARNGANDDLYPWGKTWQADK
                     AVVKEAAPFDVGSRPDGKNKWGVVDLLGNVWEWTSSRVSAYPGNPITIPDSTKDWVTI
                     RGGCYVSDPANLDKPVSSCLREFVPPATKNTLLGFRLVRSAS"
     gene            complement(8139..>8852)
                     /locus_tag="VFH91_00185"
     CDS             complement(8139..>8852)
                     /locus_tag="VFH91_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6861419.1"
                     /translation="DQMISAFGADASRLFVLFAAPVENELRWSETGIEGAVRFLRRVY
                     SAVYKWTDRLADAPSTVDNEELSTGARALRRKTHQTIAKITSDFDQLHLNTSVAALME
                     LFNQLTDLNVDPSRASRSEVFVVREALEALVVMLAPFAPHVGEEMWEGLGHTNGLLTG
                     RAIWPTADPELARREELEIPIQINGKLRSRLITAPEVSEDELRAAALADDKVKALIDG
                     QEVLKVVVVPRRLVNIVVR"
BASE COUNT         2074 a         2295 c         2276 g         2207 t
ORIGIN      
        1 taaagactct tgtgtccgaa gcctctatcg caatggcagt aacacggaag ttgcgcgatc
       61 gtttggggga tttcgcgcac cacaatgtaa gcctctttgg ccttgccaaa aaattgctca
      121 ggcggaagcg ttggaccaag gctggctcgc gccggcgcct cttcgaagtg ggccggtatc
      181 gcattcctct cgaggggttg ctctgccggt gaatgagaat gtgacggcgg ggcgcttact
      241 ttcgcttgtt cgtgaccaga tgaattaaaa atcaacacaa cggccaccac taccagggcg
      301 acaccgagga tcaacaacca gtttcttttc atgaacttct cctgaaatgt ttagtgcata
      361 tcgtgcgagc cggtcatctt caataaccaa acccaacgat agccctttga tcacagacac
      421 tgtttagaac tcctaatacg aggacgtcgt gcggtttaaa gcacggcgtc ctcattcatt
      481 tgctggcctg ccgtttattg cttcggcttt tcagacttca tgctgtcgtg cttcatcttc
      541 atgttgcagc aactggcacc tgctgactga ctcatttgac ttccctcttt catgtcgcat
      601 gaatcgcctt tacagcagca ctcgtgaccc gaggagtggt ttgcttttgc atccatcttc
      661 gtcatatcgc acgagtcgcc gtcgcaacag caacatccgc tcgaagccgc gtgatgcttc
      721 atgcctttgt tctctgcgtt catgtcgcat gattcatcac aacaacaacc agacttttgc
      781 ccatcagttt tcgcaacaga agtagcgtct ttctggtgag cggcacaaca acacgaatcc
      841 atcgtgctgg cgtctttggt ctgcttttga ttttggctgg attgggtttg agcgagacca
      901 actgcagtga acacaactag cagtagtccc atcagaataa aagttgtact agttcgtatt
      961 ttcatatcgt tctcctgaat taaagatcgt tcgacggcca gtcaaagaca gactggcaca
     1021 aagagaaaac gcacgcatcg tttatctcga tatcgaagga gtatttaaat caggaaaacg
     1081 cagtgaagaa ggtgagtagg accacgatta ggtatggata gcggggctga tgcagctctc
     1141 ggactgatat tcgcaaaatc gaaaccggga accgggctgt gaagcgatag gaccgcggtc
     1201 gcatcgctct ttaccggaga gaccgcggca ctttggttca ccgctagcgg acagtcgctc
     1261 atcatccccg aaggaactgc atttaccgac aacttctgca gttgtggtgc ggccttgtcg
     1321 agcttgctcg gttgtttctt tgcacaacag tcgtgcgcgt gcatcgaatg gcaactggtt
     1381 cctgcaacta ccgaattcaa agaagcgcga gcttgcacaa cggcgcccga accatagcta
     1441 cagccgaaca gacatcccgc tcccgctaac cataaagaaa cgcaggtagc gagcagtagc
     1501 cgaacgagtc ggattgggcg gagcaaatcc atcaagccaa gtttagcttt caccttaaga
     1561 atttgtcgag agagtgtgat ggatcggtgg aaatcgctat cgaagggcga tatgacttta
     1621 aattgccatg aaatcccacc cgttctgaaa gaacatcctg ttaagtttta accatcaaac
     1681 aagcccaagg gaggatttaa atggggatag ataaaaacaa gatcaagaca caggatgatt
     1741 tgaatgagcc cgctcctatg gatcaaatcg aagcgcgggt cgacgcaaaa atgaaaagat
     1801 tagagggtaa agcgaaacaa aacgtcgggg aaggtttgag cgataactcc ctcgcacgcg
     1861 agggagaaaa actgagaaag gagggtgaga aagcactaaa taaaacaaaa aatagttaag
     1921 ctaaaaacct caagggcccg attatcgcaa ttatcaatga gaattgttaa ttgagattcg
     1981 ggcccttgag tcttacccgt agatcactac tcagccagtg tagttgagtg cttgttctaa
     2041 agcttctctt atctggggtg gggtaagctg gggatggaat ccgataaatc tctttaggat
     2101 gtgcccatca cgagtaatga taaagctctg aggaattgaa cctctgccct gcatcaagat
     2161 tgctgcaact tcaggtgtgg cccaaccgat gtggtagtcg acgttgttgt ccttcacgaa
     2221 ttgcctgacg ctttgagccg aggcatcggg gtcttccgtc gaaagaccga caacctcaac
     2281 gccgcgcgat tgaaattcct tgtgcaggcg aaccaattct ggcgtctcaa tccggcatgg
     2341 accacaccat gtggcccaca aattcaccaa catgacttta cccgagtagt cagacagttt
     2401 gatttgagca ccgtttgctg atctcatttg cgcctgaagt actggcggtg gcagcgcact
     2461 caatcctggt gaagcggtct gctgaggagc gttagctggt gcgctatttg tgggaccagc
     2521 aaccggactt ggatcattcg agttacagct cgaaactcca aaggcggcga ataagcccaa
     2581 cacaatgatg gtcgccacga tacgaagcgg actccagaat ccattgattg gggcctttga
     2641 ttctttagag gacatatttc tttagcctga ctgcaaactg cgaaaaaaca tcgggagtcc
     2701 ggcgaaaatc acttcttgcc gaacctgaac gaatcgagaa ttactggcgc ttcaccttta
     2761 tcgccgacat cttctataga tgacagctcg ggcgcgagag atgtcgtaag catcgttaaa
     2821 gttgcgcctg ctgtgtcgcc ctctttgccg gtgggaagga agatgactcg accccataaa
     2881 tggagatcgc ctttttcagt acctcttgaa aatccttccc atctaaactc ataaccttgc
     2941 atcgagttta ctttggttgg tccttcggag attttcttat attccggaat gttcttagcc
     3001 agagcggcac tcaattcttc gactcgtttc gggtaagtcg gtagatcgcc gataaaagta
     3061 ccgctactgg tgtaccaacc aactgcgaaa ttttcttgag taaagtctgg tggaatactg
     3121 cgttctactt tgacgaaatt tgaagagcca ccgacaccgg ccttgggatc ggattgccag
     3181 ttcttggggt aataaaaaga gaaatcaaag tagtgttctg cgagtcggcc atccagcttg
     3241 tcttttgagt tcgcgaactc gacggtattt ggtggtggaa cgaagttgtc ttcgggcgac
     3301 ggcgatggtt gctctgttgg cgacggctgg gcgttcacat ttgcagtagt gttagtgttc
     3361 gcgggtaccg gagtcggctc aggaaccttg tttccggcca tttgatccaa tcgcggtttg
     3421 acgacaaaga aaaacacgcc cgccaaggct ccaccgccga gcagcaacag caacaaaacc
     3481 cctgcgataa tcaacggcag ttttgatttc ttcgctggtg cagctggagg cctcgctgcg
     3541 ggagcagacg gtaatggggc cgaaggcgat tgagccaccg gactctccaa gatcggaggt
     3601 ggcggcggtg gcggtggaac ggtggtggcg atagtagctg cgagcggcgg aggtggcggt
     3661 ggtggagcgg ccggaggtgt cgctggcggc tgaggctggg gtggcgcaat gtctgctgta
     3721 tttttcggac tcgacgttgg accagtggta ttccgcggca aggcgatagt tgccgggtac
     3781 gattccatga tcgttgcgtt tggatccgtc agctcgcggc tggtggactt ttccgacgct
     3841 ggcgtcaaat cggctgatgt cgcacgaatt gtcgcgtttg gatcgaacgt ctgcggcgca
     3901 atcagtaagc cgccatcaaa agtacagaac ttttgtccct catccgggaa cgtacgatta
     3961 catttagggc aactcttcat acacgagacc tcttgatgga gtggattgat tcaaaggcga
     4021 tcaatttgca cgagctgtcg caagcttctg agatatacca ccgtttcccg gatctagttc
     4081 gagggcacgc tggtaagctt tcgcagcatt cgccttctgt ttagctttca tgtacccgtc
     4141 acccactcct tcgaaaccat agattcccgc caactgtttt cgctcaccag gtgcagcaaa
     4201 ctcagaagcg cgttcataag tggtagcagc gtcatcaaac cggtccattg ccaggtaaac
     4261 ccggcccagg agattgtacg gttggtaacc gcgcggacta acttcagtcg cagttttgag
     4321 gtagggttct gcttccgaaa atcgcttgta aacaattaac aagcgcgcca tcaatatcaa
     4381 ggacgtcgga ttccgtccgt caagcgacaa cgcttgtttt agtacaggct cagatgaagg
     4441 ccacagttgt tcagcctgca gcgattcggc gatcaagtta agagtggcag ggtctttggc
     4501 aagttcggtt gctttggcat acaacgggac agcttcagcc tgacggttag ttcggttgag
     4561 cactcgagcg agatgggcgt acgctacggc gaactgggga tccaattcaa tcgcgcgtcg
     4621 atagcaatgt tcggtttcgc tgttgacgat tcctcgcgct tgtagggcca cacccagtcg
     4681 atcccacccg acagcgtttt gtggatcaat ctggaccgac cgacgcgcag ccatttctga
     4741 atccgcgaaa cgtttggcat tgtcgccccc cggctttggt tgtaccagca ccacactgag
     4801 cgcaaggtat gcatcggcat tgtttggaga ccactggatc gcgttgcgat aggccgtctc
     4861 ggcgtcatcc cacttctgct gatcagaata gacgttaccg agtccataag cagcgcgagc
     4921 atcctttggt ttaattttca gaatgctctg atatgcattt tcagcctcgg cgtatttgcg
     4981 agcgtcacga aacccattgc ctttttcaag gagttcttcg actctttctt caactgcagg
     5041 ggctttgggc cgactggtag cgggacgatt ggatggtttg gtgggggttg ctggcgactt
     5101 cgacttcgtt tttgtttcgg gattctttgc tcgaaaaata cccgcgccgc ctcccacatc
     5161 tgccccaaca tcctgtgcca aaacggcact ggcagcaagc aaacataccg aaaaacaagc
     5221 tgccgaaagg acaagcatgc gcaaagttgc gttagctctc attctggtct cctacggtct
     5281 tattccttgg attatgacaa gagatcgact ttgagtactg ggctcaaatc gcaaaatgcg
     5341 ttccttttac tcgacaaccc agttttcgcc aagaataatc tgttcggatt gtagatcagc
     5401 ttttgcggat gagctaattg gatttcgact gattcgaccc ggtggacgct cctggtggct
     5461 tacgaggcgc tccgaacgag tcgaaaaccg agtaaggtgt tctttgttgc gggcgggacg
     5521 aactctcgca ggcatgatga aaccggcttg tccaggtttg ccggatcact tacataacac
     5581 cccccacgga tcgttaccca atcctttgtg ctgtctggaa ttgtgattgg attccctgga
     5641 tacgccgaca ctctagacga agtccattcc caaacgtttc ccaaaagatc tacgacaccc
     5701 catttgttct tgccgtcagg ccgcgaaccg acatcgaatg gagcggcttc tttaacaact
     5761 gctttatcag cttgccacgt ttttccccat ggatatagat cgtcgttcgc accatttcgg
     5821 gctgcgtact cccattcttc ctctgtcggc aaacgataag taagggagtc acgttttgag
     5881 cgccagccgg caaaggcgtt gacatcgtcg aatgagacat taacgacggg ccacttctcc
     5941 tgaccaaacg gcggctttgg actgccccaa tgtgatggat acgcatgatt ggtctcacga
     6001 acgaaatcgg cgtactcggc gttcgttact tcagtccgat ccatcagaaa tccgcgaact
     6061 tcgatcgggt gtcccggagt ttcctgacgt ggtcccctgt ctcttcccat catgaatttg
     6121 ccgccatcta ttgcgagtag ctcagcttta actgttgtcg gcttgtcagt tgacggcggt
     6181 ggttgagtcg tcaccactgc agcgggcttg ggtcgaaaca tcaggtatcc accaattcca
     6241 atacccgcga gtacaaggat gacaatgcca gcagctacga gtagaaccgg aacggccttt
     6301 ttagttccgc tttccggcaa aacgtgtcca gcttcgacag gaataccgcc ggttatagct
     6361 tgcgtggttg tcgcaactcg ctggacatga gttgtttctg gatcgattgc cgcagccggc
     6421 gacacgctca cggacaggcg ctcgagacga tcttcaagtt ctttggcttg aactgccatc
     6481 cgctcgaact tctcttgttg ttcgcgctca cgagcttcgc gttctttctt cgcggcttct
     6541 tcgagttttc gtctttcctc ttctgctttt ttcccttcga gtcgcttttg ctcttcccgc
     6601 gcaaaacgtt cttgctcttc tttttctcga cggtgcgcag ctgcagtatc agacagctgt
     6661 tgcttgacgt tcgtatccaa ctcggacgcg ctgaccgtac ccgcgagaga ttcaaaagac
     6721 gcagcgaatt gtgtgtcgtt agtatctgga gattgagaac cggtagctaa cagtggatcg
     6781 gcctggtttg ttgttgagga gaacgcagac gcaatggtct gattgggatc gaggccgcca
     6841 gtttgccttg gcttactcgc cgggcctggc gaagaattta aggctgcgtg aagctccttg
     6901 aggaatgtcc caacggactc tattcgagcg tctgcttctt tctcgagcgc atggcggact
     6961 accccttcaa ctgccggtgg cactgaaaca cccatcgatt ggaagctcgg cggcggcagg
     7021 gtcagatgct ttttcatgat cgaagggatt gacgatcctt tgaacggaac ctcaccgcaa
     7081 agcatctgat aaaggattac accgagactg taaatgtcgg ctcgggcatc cggctcatca
     7141 tcggaccatt gctccggagc catgtagaaa ggcgagccca taagaccact cgtctgagct
     7201 tgcacgaacg aacccaataa ttctccggat ttgatcttcg ccagaccgaa gtcgagaatc
     7261 ttgatacctt ccgcaaccgg gacctcgtcc tgaagcatta tgtttaatgg tttcaggtcg
     7321 cggtgcacaa tcttttgacg atgtgctgct cccacacccg ctgcaacagg actgatcaat
     7381 tcccaggctc gttcaggcga catagcacct tcctggccga gaacatcttg aagtgatctt
     7441 cccttgataa actccatcac aaggaatggc atcgtgcctc ggaccacacc gaagtcagtt
     7501 accgcgatga tgttttgatg tctgatcgct gcagccgcaa gtgcttcttg acggaatcgc
     7561 gtcgccaaca tcggatcgtt tccgaccagg tctggaagaa ttacttttat ggcgtgagag
     7621 gttttcagga agatatgacg agcctggaaa acaacgccca ttccaccctg tccaagacgc
     7681 ctctccagaa cgtatctgcc atcgagggta ggttcaccca ctaaagtaga ggcggtcaca
     7741 tcaccatcct gtggacagtg attgatctcg tcggggaaac agcgcttaca gaccggacat
     7801 tctttcatgg gatggctcgt aacactcggc ggctaaatca taatggcaag gcacgtccac
     7861 ttgtcgcaaa gctttggttc ttcggatgca acagggagtt tttcaaagca attggacttg
     7921 aaaaacagtg gtcggcagga cgtttcgaga aatctggaaa ctgagtaatt gatcatctgg
     7981 taattcgagt gatttccgcc acgttcgggt aaagacaatc gcaggctgat tttagctact
     8041 agatcggatg caagcaaccg cttttgcctt agtagccttt ctaacaccag atgtcgtcga
     8101 agcaggacgg ccactcaggg ccgtcccgac ggtcgacttt atcggaccac tatattgacg
     8161 agacggcgcg gaaccacgac tacctttagc acttcctggc catcgattaa agcttttact
     8221 ttgtcatctg ccagtgccgc agctcgcaat tcgtcctcag agacctccgg ggcagtaatc
     8281 agccgcgagc gaagtttgcc gttgatctgg atcggaatct ccaactcttc acggcgcgcg
     8341 agctccggat cggcggtcgg ccaaatagct cttccagtta acaggccatt cgtatgccca
     8401 agaccttccc acatctcctc accaacgtgg ggagcaaacg gagccagcat tactacgagc
     8461 gcctcgagag cctccctaac tacaaacacc tctgatcgcg aggctcgact cggatcgacg
     8521 ttgagatcgg tcaattgatt gaataactcc atcaatgccg ccacgctggt atttaaatgg
     8581 agttgatcga agtctgacgt aatcttcgcg atcgtttgat gagtcttacg tcgcaaagct
     8641 ctcgcaccag tgcttaattc ctcattgtca acggtactgg gagcgtcagc cagtctgtcc
     8701 gtccatttat acactgcgct gtacactcgc ctcaggaatc gaacagctcc ttcgataccg
     8761 gtttcggacc atcgcaattc attctcaacg ggcgccgcaa acaaaacgaa caggcgactt
     8821 gcatcggcac caaaggcgct aatcatttgg tc
//
LOCUS       DASSFP010000009         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_299738, whole genome shotgun sequence.
ACCESSION   DASSFP010000009 DASSFP010000000
VERSION     DASSFP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299738"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1242
                     /gene="metK"
                     /locus_tag="VFH91_00190"
     CDS             91..1242
                     /gene="metK"
                     /locus_tag="VFH91_00190"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET6861420.1"
                     /translation="MTTESGYLFTSESVTEGHPDKISDQISDAILDAALSVDPKARVA
                     CETLVTTGLVVVAGEITTDAVIDYSRIARETVREIGYTRAKYGFDCDTCAVLSALDRQ
                     SPDIAMGVDTGGAGDQGLMFGFACNETPELMPLPIQLAHLLARRLSDVRKSGELPYLR
                     PDGKSQVTVEYRDGRPFRVEAVVISSQHDANVSNEQLRSEIEEKVIKHTVSPELLDKS
                     TKYHINPTGRFVTGGPQGDAGLTGRKIIVDTYGGYAPHGGGAFSGKDPTKVDRSAAYM
                     ARYVAKNIVAAGLADRCLVQLAYAIGVADPVSVLVDTAGTGRISDTAISKLVREHFEL
                     TPRGIIEELNLRRPIYKATAAYGHFGRNEEGFTWEKTDKAEALRSTAGI"
     gene            1274..2551
                     /gene="ahcY"
                     /locus_tag="VFH91_00195"
     CDS             1274..2551
                     /gene="ahcY"
                     /locus_tag="VFH91_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET6861421.1"
                     /translation="MSAKSSVEFDVKDLGLASNGKQRIEWAEREMPVLRLIRERFGDE
                     KPLSGVKLVACAHITTETANLARALQAGGAEAILIASNPLSTQDDVAASLVSDWGIPV
                     YAIKGESTDTYNRHVRTALDSHPDIIIDDGSDVVATLLKERADQVKEIIGSTEETTTG
                     IQRLKAMETANVLPFPSIAVNNAQTKHFFDNRYGTGQSTLDGIIRATNILLAGRNLVV
                     VGYGWCGKGVALRARGMGSNVIVTEIDPIKAVEAVMDGFRVMPIGEAASIGDIFVTVT
                     GNRHVIDGEHFSKMKDGAIVCNSGHFDLELNLVALREMAGDPVTMRPFVEEYKNKSNG
                     RRVIVLGEGRLINLAAAEGHPASVMDMSFANQALSVEYLIKNKGILKPGVHLLPAEVD
                     TEIASLKLRALGISIDTLTAEQLEYISSWETGT"
     gene            complement(2553..>3041)
                     /locus_tag="VFH91_00200"
     CDS             complement(2553..>3041)
                     /locus_tag="VFH91_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET6861422.1"
                     /translation="WVDPSHLKLLNSETLSLKGMEVEKTIEPETTLDSVESPQIFKPS
                     IDKPSQMSAVGLNTYLKAAKRRGSDVSALSVALQRKYAAPFSVLIMAFIGMPLAVSFG
                     RRGTIIALCSAVVVGIAYWAVGGGFQQLGNLGLLRPSVAGWAPLLIFTAAGTYFLSRV
                     RT"
BASE COUNT          782 a          785 c          773 g          701 t
ORIGIN      
        1 ggccctcctt gacactgttc gagccagact gctactctac gcggtctttt tcttcgctga
       61 attcgagact gtacatacaa aggagttgat ttgactaccg aatccggtta cttattcacg
      121 tcggaatccg ttactgaagg acaccccgac aaaatctccg accagatctc tgacgctatc
      181 ctggatgctg cactttccgt tgaccctaaa gcgcgtgttg cttgtgaaac cctggtgacc
      241 actggccttg tcgtggttgc cggagaaatc acaacagatg cggtgattga ctattctcga
      301 atcgctcgtg agacggtccg agaaattggg tatacacgcg ccaaatacgg ttttgactgc
      361 gacacctgcg ccgtgctgtc agccttagat cgacagtctc cagacatcgc catgggtgtt
      421 gatacaggcg gcgccggcga ccagggtttg atgttcgggt ttgcttgtaa cgagacgcct
      481 gagctgatgc cgcttccaat tcagttggca catcttctcg cacgacgcct ttccgacgtg
      541 cgaaagtcag gcgaacttcc ctaccttcgg cctgacggaa aatcacaagt taccgttgag
      601 taccgagatg gcagaccatt ccgggttgaa gccgtcgtca tctcctcaca acacgacgcg
      661 aacgtctcta acgaacagct aagatctgaa attgaagaga aagtcatcaa acatactgtc
      721 tctcctgagc tgctcgacaa aagcaccaag tatcacatca atccaactgg ccgttttgtt
      781 accggcggac cacaaggcga cgcaggctta accggtcgga agatcattgt agacacctat
      841 ggcggttatg cgcctcatgg tggcggtgct ttttccggca aagatccgac gaaggtcgat
      901 cgatcggccg cctatatggc gcggtatgtc gcgaagaaca tcgtcgctgc cggtcttgca
      961 gatcgatgcc ttgttcaact ggcttacgca atcggtgtgg cggatccggt ttcggtgctc
     1021 gttgatacag caggcacggg tcgaatcagt gatacagcta tctccaaact cgtacgtgaa
     1081 cacttcgagt tgaccccacg aggcatcatc gaagagctga accttcgtcg tcccatctat
     1141 aaagctactg ccgcatacgg ccactttggt cgaaacgagg aaggctttac atgggaaaaa
     1201 acagacaagg ccgaagcact tcggtccact gctggaatct aacttctgaa atcagataag
     1261 gattaaggaa aatatgagcg caaaaagttc cgtcgaattc gacgtgaaag atcttggcct
     1321 ggcaagtaat ggcaaacagc gcattgaatg ggccgagcgc gagatgcccg ttctgcgatt
     1381 aattcgagaa cgctttggag acgaaaaacc gctgagcggc gtgaagctgg ttgcttgcgc
     1441 gcacataaca actgagacag caaatctggc tcgagctctt caggcaggcg gcgcagaagc
     1501 aattctcatc gcctcaaacc ctctctcaac tcaggacgac gtcgcagcgt cgctcgtttc
     1561 tgactgggga attcccgttt acgcaatcaa aggcgaatct actgacactt ataaccgtca
     1621 cgtgagaact gctctcgatt ctcatccgga tatcatcatc gatgatggtt ccgacgtggt
     1681 tgccaccctg ctaaaggaaa gagcagatca ggtcaaagag atcatcggat ctaccgagga
     1741 aaccaccacc ggtattcagc gcctgaaggc gatggaaact gcgaacgtac ttcccttccc
     1801 gtcgatcgct gtgaacaacg cccagacgaa acacttcttc gacaataggt acggtactgg
     1861 acaatcgaca ctcgatggca tcattcgagc caccaatatc ttgttggcag gaagaaacct
     1921 ggtagtcgtc ggttatggct ggtgtggtaa gggcgttgct ttaagagcac gcggcatggg
     1981 ctcgaacgtg atcgtcaccg agatcgatcc aatcaaagcg gttgaagcgg taatggacgg
     2041 tttccgtgta atgcccatcg gcgaagcagc gtcaattggc gatatttttg tcactgtaac
     2101 aggtaatcgt cacgtgattg atggtgagca tttctcaaag atgaaagacg gcgccattgt
     2161 ttgcaacagt ggtcacttcg atttggaact caatctcgtc gcgcttagag aaatggccgg
     2221 cgacccagta acaatgaggc cgttcgttga ggagtacaag aataaatcga acggtcggag
     2281 agtaatcgtc cttggcgaag gtcgtttgat taacctggct gcagcagaag gacatccggc
     2341 cagcgttatg gacatgagct ttgcaaatca ggcactgtca gttgagtatt tgattaagaa
     2401 caaagggatt ctgaaacccg gagttcactt gttaccagcc gaggttgata ctgagattgc
     2461 gagcttaaag cttagggccc tcggaatttc tatcgacaca ctgaccgccg aacaactcga
     2521 gtacatctcg agctgggaaa ctggcactta actcacgttc tcactcgtga gagaaaatag
     2581 gttcccgctg ctgtgaatat cagcagcggg gcccaacccg caaccgaagg tcgcaacaag
     2641 cccagatttc ccaactgttg gaacccgcca ccgactgccc agtaagcaat tcccacaact
     2701 actgccgaac agagtgcgat gatagttcct cgtctgccga acgacaccgc cagaggcatt
     2761 ccaataaaag ccataatcaa aacgctgaac ggtgctgcat acttacgttg cagagcgaca
     2821 gataaagcag atacgtcaga gccgcgtcgc ttggcggcct tcaagtaagt gttgaggcct
     2881 actgcactca tctgcgacgg cttatcaata ctaggtttaa agatctgcgg tgactcgact
     2941 gaatcaaggg tcgtttccgg ctcaattgtc ttctctactt ccataccttt caaagataag
     3001 gtttcgctgt taagtaactt taaatgtgat ggatcaaccc a
//
LOCUS       DASSFP010000010        10223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1490359, whole genome shotgun sequence.
ACCESSION   DASSFP010000010 DASSFP010000000
VERSION     DASSFP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10223
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490359"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..645)
                     /locus_tag="VFH91_00205"
     CDS             complement(64..645)
                     /locus_tag="VFH91_00205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET6861423.1"
                     /translation="MIGQLDFEKVVLASGSPRRKEIMTAVGWPFEIRTPDVDESVRPA
                     EDPVDYVQRLAQVKALTVADQLEAGLVLAADTTVVVNGEILGKPEDDDDARRMLRLLN
                     GKWHEVLTGVSLVRIKGATLTSFERTRVRFAEMTQREIDWYVSLGEPQGKAGAYGIQG
                     PAALFIEEIEGDYLNIVGLPIRLVYRLWQELPR"
     gene            754..1206
                     /locus_tag="VFH91_00210"
     CDS             754..1206
                     /locus_tag="VFH91_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HET6861424.1"
                     /translation="MDNLIKSLPGVLSAARASPEVSQAAAFAAFTHTAGEGLRSHAVP
                     RQLNDDGTLVIAVSDVLWQKQLKSMLSELIFRTNTLLGSAIVKNIEFQIDPLLVKSKR
                     PTTEAVEEIKETDVPLEVWAAANAIHDRELRLKFLRTAVIAIKRQEKK"
     gene            1210..1509
                     /gene="gatC"
                     /locus_tag="VFH91_00215"
     CDS             1210..1509
                     /gene="gatC"
                     /locus_tag="VFH91_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET6861425.1"
                     /translation="MPISENDIAKVAQLAHLELADDELKVFVPQITEIVSYVEQLNEL
                     DTHNVVPATGGLTPEGENTDSSRADEALPCLGQRTALAEAPAAAEGHFRVPKVLT"
     gene            1518..2906
                     /gene="gatA"
                     /locus_tag="VFH91_00220"
     CDS             1518..2906
                     /gene="gatA"
                     /locus_tag="VFH91_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET6861426.1"
                     /translation="MFRESIEFSINAAEKLNDTLNAFLQIDRKGALQRADELEGSTRG
                     LLAGLAVAVKDNICVKGLQTSCGSKILGAYHPPYNATVIERMRNAGAVIIGKTNCDEF
                     AMGSSNENSAFGPVRNPWDTSKVPGGSSGGSAVAVAAGIVPVALGSDTGGSVRQPASL
                     CGVLGLKPTYGRNSRYGLVAFASSLDQVGVFARNAEDVARVLTVIAGRDPQDATTADV
                     PVPDYQKALDGDLKGARIGFPNALFGKGLDSEVAAAVKEVVEVYKELGAEIVEVELPH
                     ASYAIAVYYIIATAEASSNLARFDGVRYGFRAEDASELRQMYRKTREEGFGPEVKRRI
                     MLGTYVLSAGYYDAYYRKAQQVRTLIKNDFMTAFKQCDAIITPTAPTPAFAIGEKVDD
                     PLAMYLNDIYTVTANLAGVPGLSVPCGISSDRLPIGFQLLGPYWSESTLLKLASGYER
                     ARPFSDRPPIHA"
     gene            complement(2955..3224)
                     /locus_tag="VFH91_00225"
     CDS             complement(2955..3224)
                     /locus_tag="VFH91_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861427.1"
                     /translation="MTLKRRVMRAGGWRLAKRLIKPLPVVGTVLAVGLAGYEIKRKGL
                     IPGAIHVGLDATPLVGTAKGVLEIFTGDLIPDKKPETRPTTRTLR"
     gene            3426..4463
                     /locus_tag="VFH91_00230"
     CDS             3426..4463
                     /locus_tag="VFH91_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6861428.1"
                     /translation="MRLKLLFSLVVTILLILAFVSCRGRSSANRLLIYTPHGQDLLRD
                     FVARYKQQNPNVEIQFLDMGSREILERLRVERNRPQADLWWGASHTTFQSAAEENLLT
                     EFRPTWADKVPVSSRDEKDRWYGTYETPQVIAYNRDVVSASDAPRDWDDVLDPKWRDK
                     VLIRNPNPSDTMRAIFGAMIWRFYKDTGSTEKGYEWLRKLDANVHEYTADGTLLMQKL
                     ARHEGSITLWDMPDVRLHQEQKNLPVAYVVPASGTPLIIDGIAIVRGGPNEQEARRFY
                     EFVTTPESLVYAAHKYYRIPIRTDLDRSQLPAWMNEPFKIMPLDWDLLRKQGGDWLRY
                     WDTQIKSRSNH"
     gene            4606..5697
                     /locus_tag="VFH91_00235"
     CDS             4606..5697
                     /locus_tag="VFH91_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6861429.1"
                     /translation="MDQYPVPLLLLQNISKSYGDTRAVSGISLNVERGEFFGLLGPSG
                     CGKTTTLRMIAGLETPDAGTIQLQSADITNLPPERRGFGMVFQNYALFPHLNVFENVA
                     FGLRARHKPKNEISEKVSSALELVQLPGYEKRRVDELSGGQQQRVAIARAIAIEPDLL
                     LFDEPLSNLDVSLREETRSELRELVTRLGLTAVYVTHDQEEAFALCDRISVMVGGQLM
                     QTGKPRELYDEPSNIAVARFLGRNNLIRAMRLSSSKDSHGEFKTLEGGHTLRLPVKHE
                     DLAPLNQPVFLAIRPEHVRIVRQTETDVNRLQARVKEIVFAGATSSVRVDSNGLTLEA
                     LVLEPNGLKVGDSCEVVLSTNHLMLLKGQ"
     gene            5717..7414
                     /locus_tag="VFH91_00240"
     CDS             5717..7414
                     /locus_tag="VFH91_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET6861430.1"
                     /translation="MPSPRSKVFSARLLLLFITLIVIVYGILYPNLAVVSASLMRGGS
                     WSLANYRDLLSQRVVLEATINSVVLSVATVLLCALIAVPLAFLFERYSFPGRRIFAAL
                     AALPLVLPPLVGTVAFIFLCGESGILARVWQHIFATQNSPWTLRGWTALLLFHTYTMY
                     PFFYVLTGAGLRRINASLPEAARSLGASRLTVLRRVLIPQLRPSLIAAALLTFMTSMA
                     SFSAPLLFGGNVRVLTLEIFTARQRGDLTTAGTETVMLAIISLAALVVFQKYEGTRRF
                     VAAGLKGTARRREPIASGRSRVLATIAGVTFSILLVLPILTLIVVSFAQEGSWTTQTL
                     PSVYTLANYHRIATEPQSAEVFVNSLSMSALAAAASLVWSFCVVTLLIKRTSRGGFGE
                     TWRRLLSLIILVPWALPGTVVAVSVAEAYGQPGALLGSFVLVGTFWILPAIYFLRFMP
                     LVVRAVQASSEQVDASLEEAAGSLGANALRRFFKVTLPLVWPGAIAGTLLAFVIALGE
                     YVASVLVFVPTNRPISIAIASELRDFNLGAAAAYGVVLIGIITVSMFVAAKLERWRG"
     gene            7458..9965
                     /locus_tag="VFH91_00245"
     CDS             7458..9965
                     /locus_tag="VFH91_00245"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6861431.1"
                     /translation="MASIGLRRNALAIALLFVLLSSSELSLLKAGAQGDSNDGAGRAE
                     LHQALLDLTNPWTVMCVAAHPDDEDGSTLTILRRKYGVHTVSLFSTFGEGGQNAVGPE
                     LYEELGVIRAKETIAAAKVQGSEPHFLGLRDFGFSKSAEEAFRAWGEKEALRRMVLQI
                     RLLRPDVIITNHDTVSGHGHHQATGRLILQAFDAAADPKQFPEQLSEVAVWQPQRLFV
                     RGFRGPQTATSTTDSNNQEVTIDPNETDPIRGTTYAQQALTALQQHATQGPWPKSIGP
                     NGGRIIRYNLVRKAANAAPLPANAKTPLDGLRLPEPETARLVAPTIEGKPLTDFLDKR
                     SVVLASLVNARKLGAFTATKEITGSDAQRFQLMAGRLDAALATAANVALTVTSADRVL
                     VPDHKTPFDVTLTNSGEEEVFIKGWKSQGLSVEKKLETAEKLLPGTETSQQLMALTPR
                     SAKFSVPSAEHLYDGRLFGDPFEVDTDVEVDGAQFSLSAKKYMDVAPPVEITNNNPTP
                     CVETPATVLACESTSLTLTNNLDSQFQGVLELTDAKARHAPMKTQPLSLKPHESLKVV
                     NGPIKPVPSRGLLSFKHDGPITVKVLDGTSKQSISELTIPNIYIPAIAVAGIRVGFVP
                     STDPTLKNSLTALGVEKEELTGDSIAKADLSRFDTIIIDNRGYEIHPELIKSNDRLLK
                     FVEDGGTLLVFYHKTTEWNPDERRSRPQLAPYPIILDDQRVTEEDAPVRFLQPRHRLL
                     TFPNPITQKDFDNWVQERGLYFPKEWDPRYAALLSTNDKGEKPLTGGLLVADYGKGHY
                     IFTSFVWYRQLRAAIPGGYRVFANMISYGKRSNTNQK"
BASE COUNT         2477 a         2594 c         2610 g         2542 t
ORIGIN      
        1 caacggtcaa gcaatcaata ttgatggcgg cactgtgctg ttttagcgga ggtttattag
       61 aacctagcgc ggcaactcct gccaaagccg atagaccaac cttatcggta aacctacgat
      121 gttaaggtag tcgccttcaa tctcttcgat gaagagggcc gctggtcctt gaataccata
      181 agcgcctgcc ttaccctgcg gttctcccag tgacacgtac caatcaatct ctctctgagt
      241 catctccgca aaccgtactc gtgtcctttc aaagctggtt aacgttgcac ctttgattcg
      301 cactaaactc acgccggtga gcacttcgtg ccactttcca ttaagaagcc gcagcattcg
      361 tctcgcatcg tcatcgtcct ctggtttgcc aagaatttca ccgttaacca cgactgtcgt
      421 gtccgcggcc aacacgagac ccgcttccag ttgatcggcg acggtcaaag ctttcacctg
      481 tgctaaacgt tgtacataat ctactggatc ctcagctggt ctaacagatt catccacatc
      541 gggggtccgt atctcgaacg gccagccaac cgctgtcata atctctttcc ggcgtggact
      601 tcctgatgca agtacgactt tttcgaaatc taattgtcca atcaatttaa ctcccgtttg
      661 ttgtgttctg gattcttcgc tacactgctc aagcactgaa ctatttatcc tcgaagagtc
      721 aggcgaagat cgcaaatgcg gagagactcg tcaatggata atctgattaa aagcctaccg
      781 ggcgttctga gtgcagcccg tgcttcgccg gaggtttcgc aggcagcagc attcgcggcc
      841 tttacgcaca ctgccggcga gggcctaaga agtcacgcgg tgccacgcca gttgaacgat
      901 gacggcaccc ttgtgatcgc cgtatctgac gtgttgtggc agaaacagtt gaagtcgatg
      961 ctgtcggagc tgatctttcg caccaatact ctgttgggaa gtgcgattgt taagaacatt
     1021 gaatttcaga ttgatccgtt gttggtgaaa tcaaaaaggc cgacgaccga agcggtcgaa
     1081 gagatcaagg agacggatgt tcccctcgag gtctgggccg ccgcaaacgc tattcatgat
     1141 cgagaactga gactgaaatt ccttagaact gctgtcatcg caataaaacg ccaggagaag
     1201 aagtaatcca tgccgatcag tgagaatgat atcgctaagg tggctcaact ggcccacctg
     1261 gaactggcgg acgatgaact gaaagtcttt gtgcctcaaa taactgaaat cgtcagctat
     1321 gtcgaacaac tgaacgaact tgatactcac aacgtcgtgc ccgccacagg tggtctaacg
     1381 cccgaagggg aaaacacaga ttcgtctcgt gccgacgaag cgcttccgtg ccttggacag
     1441 cgaactgcac ttgccgaagc tcccgctgct gcagagggcc acttcagagt tcccaaagtg
     1501 cttacttaaa tacagacatg tttcgtgaat ccatcgagtt ttcgataaac gccgctgaaa
     1561 aactgaacga tacgctgaac gcatttcttc agattgatcg aaaaggtgca ttgcagcgtg
     1621 ctgatgaact ggaaggcagc acgagaggtt tgctggcagg cctggcagtg gcagttaagg
     1681 acaatatctg cgtcaaagga ttgcaaacgt cctgtggctc aaagatcctc ggcgcttacc
     1741 atcctccata caatgcgact gtgattgagc gcatgcgaaa cgcgggagcc gtgatcatcg
     1801 gcaagaccaa ctgcgatgag ttcgcaatgg ggtcttccaa cgagaattcg gcgtttggtc
     1861 cagtacgaaa tccctgggac accagcaaag tccccggtgg ttcctcagga gggtctgctg
     1921 ttgctgtcgc cgctggaatc gtgccagttg cacttggctc cgatactgga ggtagcgttc
     1981 gccaaccagc gtcgctgtgt ggcgtgctgg gactgaaacc aacctatggt cgtaactcac
     2041 gctacggatt ggtggctttt gcttcatctc tggaccaggt tggagtattc gctcgaaacg
     2101 ctgaagatgt cgcacgcgta ctcaccgtga tcgcgggacg agatcctcag gatgcgacga
     2161 ccgctgatgt acccgtacct gactatcaga aagcgcttga tggcgatctc aaaggtgctc
     2221 gcattggatt tcccaatgct cttttcggaa agggtctgga ttccgaagtt gcggcagccg
     2281 tcaaagaagt agtcgaagtc tataaggaac tcggtgcaga gatcgttgaa gtcgagttac
     2341 ctcatgcaag ttatgcgatc gccgtttatt acataatcgc gaccgctgaa gcttcttcaa
     2401 acctcgcccg cttcgatggt gttcgctacg gattccgggc tgaagacgca tctgagttac
     2461 gtcagatgta tcgaaaaact cgtgaagagg ggtttggtcc tgaagtcaaa cgaaggatca
     2521 tgctcggcac ctatgttctt tcggcgggat attacgatgc gtattaccga aaagctcaac
     2581 aagtgcgcac attgattaag aacgacttta tgacggcgtt caagcagtgc gacgcgatca
     2641 ttacaccaac cgctccaact cccgctttcg caatcggtga gaaggtcgat gatccgttgg
     2701 cgatgtacct gaatgacatc tacaccgtga cggccaatct tgcaggcgta ccgggactga
     2761 gtgtaccgtg cggcatttca tctgatcgat tgccaatcgg tttccaactg cttggtccat
     2821 attggtcaga atcgactttg ctcaaactgg caagcggcta tgagcgagcg aggccgttta
     2881 gtgatcgacc tccgattcac gcttgaatag cgctgaagta tctctgcctg tctttagcta
     2941 tcagcatgta actcctaacg gagtgttcga gttgttgggc gggtttctgg ttttttatcc
     3001 gggattaggt ctcccgtaaa aatttcaagc acacctttag cggtaccgac aagtggagtt
     3061 gcatcaaggc caacgtgaat ggcgccgggg attaaacctt tgcgcttaat ctcatatcca
     3121 gccagtccca ctgccaggac cgttccgaca acgggtaacg gtttaatcag acgtttggcc
     3181 agtcgccagc cacccgctct catcactctt cgttttagtg tcatcgtttg tgctatctga
     3241 ttatacataa tgctgaaatc gcgattcaaa gagttctcag tgcttcgttt cgaggatctc
     3301 cttcaatcaa tacgtggcga ccaaaagagg cccgatttcc caattatcaa atatcaaatg
     3361 ataattgatc attaattacg tgctagcata atgacggccc aaacaatcgg acaatcgaca
     3421 cttagatgcg cctaaagctc cttttttctc tcgttgtcac aattttatta attctggctt
     3481 ttgtttcttg ccgcggtcgc tcctcagcca accgtctgct gatctacacc ccgcatggtc
     3541 aggatttgtt gcgcgatttt gtggcgcgct acaaacagca aaacccaaac gtagaaattc
     3601 aatttctcga catgggttcg cgcgaaatcc tggagcgcct tcgcgtggaa cgaaatcgac
     3661 cgcaggcaga tctttggtgg ggagcttcac acacaacctt ccagtcagct gccgaggaga
     3721 atcttttgac ggagtttcgt ccaacatggg ctgataaggt gccggtttct tcacgtgacg
     3781 aaaaagatcg ttggtacggt acttatgaaa cccctcaggt tatcgcctac aaccgcgatg
     3841 tcgtttcggc gagtgatgct ccgcgcgact gggatgacgt tctcgatcct aagtggcgtg
     3901 ataaggtcct gattcggaat ccaaatccgt ctgacacgat gcgcgcaatt tttggagcga
     3961 tgatttggcg cttctacaaa gacactggtt caaccgaaaa gggttacgag tggctccgaa
     4021 agctggatgc aaatgttcat gagtacactg ctgacggaac ccttctaatg cagaaactcg
     4081 cacggcatga aggctcgatt actctgtggg acatgcccga tgtgcgcctg catcaagagc
     4141 agaaaaattt gccggttgct tacgtggtgc cggcgagcgg cactcctcta atcattgacg
     4201 gcatcgccat cgtacgtggt ggtccaaacg agcaggaagc gcgccgattt tatgagtttg
     4261 tgacaacccc ggagagcctg gtttacgcag cccacaaata ctatcgaatt ccgattcgta
     4321 ctgatctcga ccgatcgcag ctaccggcgt ggatgaacga gccctttaag atcatgcccc
     4381 ttgactggga tttattgcga aaacagggtg gcgactggtt aagatactgg gacacacaaa
     4441 taaagagcag gtccaatcat tgatcaatta tcatttgaca attatcaatt attaagtggc
     4501 cgcgctactc tttgcttcgt tgagtctggg tattcaacaa ctgcgtaaag aacgaatgaa
     4561 tagattaata attgagaatt atcaatcgat aattctcaat tggtaatgga ccaatatccc
     4621 gtgccacttc ttttgctgca aaatatttcc aagagctacg gagacacgcg cgccgtaagc
     4681 ggaatatctc taaacgtcga acgtggcgaa tttttcggtt tgttaggtcc atcaggatgt
     4741 ggcaagacca ctacgcttcg gatgattgcc ggacttgaaa ctccagacgc aggtacgatc
     4801 cagcttcaat cggccgacat cacgaatcta ccgcccgaac gacgtggatt cggcatggtc
     4861 tttcaaaact acgcactctt ccctcacctg aatgtgttcg agaacgtagc atttggtctc
     4921 cgcgcgcgtc acaagccaaa aaatgagatc agcgaaaagg tttccagtgc gttggagctg
     4981 gtccaattac ccggatatga aaaacgccgc gtcgatgaac tctcaggtgg acaacaacag
     5041 cgcgttgcga tagctcgagc gatagccatc gagcccgact tactgctctt tgacgagccg
     5101 ctctcaaacc tggatgtgtc gttacgagaa gaaacgcgta gtgagttacg agagttggta
     5161 acaaggctgg gtctaacggc ggtttatgtt actcatgatc aggaagaggc ctttgctttg
     5221 tgcgatcgaa tcagcgtgat ggtcggcggc caattgatgc aaaccggcaa accaagagag
     5281 ctgtatgacg aaccttcaaa catcgctgtt gctcggtttc tcggcagaaa caatttgatt
     5341 cgtgcgatga gattgagctc cagtaaagac agtcacggag aattcaagac gctcgaaggc
     5401 ggacacacgt tgcgactgcc agttaagcac gaggatcttg ctcctctaaa tcaacctgtt
     5461 tttctcgcaa ttcgaccgga gcatgtcaga atcgtcagac aaactgaaac agatgtgaat
     5521 cgattacagg ctcgggttaa ggagattgtt tttgcaggag cgacgtcatc ggtgcgcgtc
     5581 gattcgaatg gattgacttt ggaagcactc gtactcgaac ccaatggcct caaggtcgga
     5641 gactcgtgcg aagtagttct ctcaacgaat cacctgatgc tgctgaaagg tcaatagcgc
     5701 attcaagcct atcaacttgc caagcccacg ttcaaaagtc ttttcggccc gtctccttct
     5761 cctcttcatc actctcattg taatcgtcta cggaattctt tacccaaatc tcgcggtggt
     5821 gtcagcgtca ctaatgcgtg gtggttcgtg gtcccttgca aattaccgcg acctcctgtc
     5881 tcaacgagtt gttcttgaag caaccatcaa cagcgtagtt ctgtcggttg cgacggtcct
     5941 gctctgtgca cttattgccg ttccgttggc atttttgttc gagagatatt cctttcctgg
     6001 tcgccggatc tttgcagccc tcgccgctct accgctggtc ctgccgccgc tcgttggcac
     6061 tgtagcgttc atctttcttt gcggtgagtc gggcattctt gcgcgggttt ggcagcacat
     6121 ctttgcgacg caaaattcac cctggactct tcggggttgg acagccttgc tcctgtttca
     6181 tacatacacg atgtacccct tcttctacgt gttaacagga gccggtcttc gcaggatcaa
     6241 cgccagcctt cctgaggccg cacgaagtct tggagcatcg agactcacag ttctccggcg
     6301 agtcctgata ccacaactca gaccctctct gattgccgcg gctcttttga cgttcatgac
     6361 atcgatggcg tcatttagtg cgccattgtt atttggcgga aacgttcgag ttctaacact
     6421 cgagattttt accgctcggc agcgtggaga tctcacaacc gcgggaactg aaacagtgat
     6481 gctggcgatc atctcgcttg ctgcgttagt agtgtttcaa aagtatgaag gaacaaggcg
     6541 gtttgtcgcg gcgggattaa aaggtaccgc tcgcagacga gaacctattg ccagcggaag
     6601 atcgagagta ctggcaacaa tcgcgggcgt cactttctcg atcctattgg tgctgccgat
     6661 tctgacttta atagtagtga gctttgcgca ggagggctct tggaccactc agactcttcc
     6721 gtcggtttat acgctggcta actatcatcg aattgccact gaaccacagt ctgcggaggt
     6781 gttcgtcaat agtttgtcca tgtcggctct tgcagctgcc gcctcgttag tgtggagctt
     6841 ttgtgtggtg acgcttctta tcaaacgaac gtctcgaggt ggcttcggcg agacctggcg
     6901 acgactactg tccctgatca ttctggttcc gtgggcgctg ccgggaacag ttgttgctgt
     6961 ctcagtcgcc gaagcatacg gacaacctgg tgctttgctt ggctcctttg tactcgtagg
     7021 aacattctgg atcctacccg caatctactt tctcagattt atgccgctcg tcgtgagggc
     7081 agtacaagca agctcggagc aggttgatgc ctcccttgag gaggcggcgg gttcgcttgg
     7141 cgccaatgca ttgcgccgat tctttaaagt cactttgccg ctggtctggc cgggcgcaat
     7201 tgccggaacc ttgctggcat tcgtaattgc gttgggcgaa tatgtagcat cggtgttggt
     7261 attcgttcca actaatagac caatctctat tgcgattgcg tcggaactac gggacttcaa
     7321 tctcggcgcc gcggctgcgt acggagtggt attaatcggc atcataaccg tcagcatgtt
     7381 tgttgccgca aaactcgagc gttggcgggg atagtatttc ggaaagcttc atgttgtttg
     7441 gaaaaggaca aaagttcatg gcatcgatag gcttacgcag aaacgctttg gcgattgcgc
     7501 ttctgttcgt attgctctca agttcagagt tatcactgct gaaagcagga gctcaaggcg
     7561 actccaatga cggcgcggga cgggccgaac tccatcaggc gctacttgat ttgacgaacc
     7621 cctggaccgt catgtgtgtc gctgctcatc cggacgatga ggatggaagt acgttaacaa
     7681 ttctccgtcg caagtacggc gtgcatactg tgtctttgtt ttccactttt ggcgaaggcg
     7741 gccagaatgc ggtaggacca gaactctatg aagagttggg agtcattcga gccaaagaga
     7801 caattgccgc agcaaaggtc cagggttccg aaccacactt cctcggtctg cgagactttg
     7861 gattttcgaa gtcagctgaa gaggcgtttc gcgcgtgggg agagaaagaa gctctgcgga
     7921 gaatggtgtt gcaaattaga cttttgcgac ccgacgtcat cattacaaac cacgacacag
     7981 tcagcggtca cggtcatcat caggcgacag gccgcttgat tcttcaggcc tttgatgcgg
     8041 cggcagatcc taaacaattt ccagagcaac taagtgaggt tgcggtctgg caacctcagc
     8101 gactttttgt tcgtggcttt cgggggccac aaaccgccac atcgacgacg gactcgaaca
     8161 atcaggaagt gacaatcgat ccaaacgaga cggatccgat acgtggtacc acgtacgctc
     8221 aacaggctct taccgctctg caacaacacg caacgcaagg tccctggccc aagtcgattg
     8281 ggccaaacgg aggccgaatc attcgctata atctcgttcg taaggcagct aatgcagctc
     8341 cgttgcccgc taacgctaag acgccacttg acggtttgcg gttacccgaa cccgagacgg
     8401 ctcgattagt cgctccaact atcgaaggaa aaccgctaac cgacttcctc gataagcgtt
     8461 cagtagtgct ggcttcgtta gtaaatgcac gtaagcttgg cgcctttaca gcgactaagg
     8521 agatcaccgg cagtgatgca cagcgatttc aattgatggc tggtcgactc gatgcggctt
     8581 tggcaactgc agcaaacgta gcgcttactg ttacctctgc cgatcgagtg ttagtaccag
     8641 atcacaagac gcctttcgat gtgaccctga caaactccgg cgaagaggaa gtgtttatca
     8701 aaggttggaa gtctcaaggc ttgtctgtag agaaaaaatt ggagacggcg gaaaaattgc
     8761 tgcctggcac tgaaacttcg caacaattga tggctttaac gccgaggtcg gctaagttca
     8821 gcgttccctc cgcagaacat ctctatgatg gacgactgtt tggagatcct tttgaggttg
     8881 atacggacgt tgaagttgac ggcgcacaat tttctctgag tgcgaagaaa tacatggatg
     8941 ttgcgccccc ggtcgagatc acaaacaaca atccaacgcc ttgcgtcgaa acacccgcaa
     9001 ctgttctggc gtgtgagagt acttcgttga cattaaccaa caatcttgac tcacagttcc
     9061 agggcgtttt ggaactaact gacgccaagg cgcgacacgc tcccatgaaa acacaaccgc
     9121 tgagccttaa gcctcacgaa agtttgaagg ttgtaaatgg gcccatcaaa ccggtaccaa
     9181 gcagagggtt gcttagtttc aagcacgatg gcccaatcac tgtgaaggtg cttgacggta
     9241 ccagcaaaca atccatatct gaactaacga ttcctaacat ctacatcccg gccattgccg
     9301 ttgctggaat acgagtggga tttgtaccaa gtactgatcc aaccttgaag aactctttga
     9361 cggcgctggg agtggagaaa gaagaactta ctggcgatag cattgcgaaa gcagatctct
     9421 cgcgtttcga tacgatcatt atcgataatc gtggttatga gattcatcct gaattgatta
     9481 agtcaaacga ccgcttgctg aagtttgtcg aggacggagg aacgctgctc gtcttctatc
     9541 acaaaacgac tgagtggaat ccagacgaac ggcgctcacg gccacaattg gcgccctatc
     9601 caattattct cgacgatcag cgtgtaacgg aagaagatgc acccgttcgc ttcttacaac
     9661 caagacaccg gctgctcaca tttccgaacc ccatcacgca aaaggacttc gacaactggg
     9721 ttcaggagcg cggattgtat tttccaaagg agtgggaccc tcgttatgca gcactcttat
     9781 caaccaacga taaaggtgag aagccgttga ccgggggact gctagtcgcc gactacggga
     9841 aaggtcatta catctttacg agctttgttt ggtaccggca gttacgagcg gcaattcccg
     9901 ggggctatcg cgtttttgcc aacatgatca gctacggaaa gagatcaaat accaatcaga
     9961 aatagtaacg ctggtgcttt cgcacgaacc actcaggggt attggttcag gttgctcgct
    10021 tttctcagcg tttctctgcg tgatctctgc gttctctgcg tcttggctgt gtagctctaa
    10081 taaggaactc cggacaagac gcagagtacg cggagatcac gcagagaacg caaaggaaag
    10141 caaactgaac cagtaccata attcgtggct gggtctttga gacttgtgat cagaaatctg
    10201 agatctcaaa ggtacgacaa cca
//
LOCUS       DASSFP010000011         5461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1937788, whole genome shotgun sequence.
ACCESSION   DASSFP010000011 DASSFP010000000
VERSION     DASSFP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937788"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1834
                     /gene="dacB"
                     /locus_tag="VFH91_00250"
     CDS             245..1834
                     /gene="dacB"
                     /locus_tag="VFH91_00250"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET6861432.1"
                     /translation="MRYLPRPFLLVLLPLLLASCSSAPETPSNYSEPIVAASSTPAVF
                     NSDASLTKTLDALLSEPEFNVSRLGVAVISLRDGKLIYQRNGDKSFTPASNMKIFTTA
                     VALDLLGADYRWRTSVYATHDVDSAGTVNGDLVLYGRGAPDLVTSSKPDNKNSLEELA
                     RSLSLRGVRRVAGNVIGDESYFRGESIGEGWQWNDLQWYFGAEASALTVNSNSVDVSV
                     EPPGKDDNQPKVSSSDSDGYVTIENNMKLVSRGDKFTLGVQKGVSDNNVTVWGEFPSG
                     ARGYGATLSVHRPSLWAARMFIRQLQAAGISVDGSAEYRDAKVPQSQRFDPHSSTELA
                     SVTGKSLGEVVHATNKLSINLYAELLLRTLGHERGALLQTEQPVGREQGDEEAGTALI
                     IFWLARAGVSTNGLALHDGSGLSRLDLVTPEATAGLLAAIRRTTSAQVFTDSLPIAGT
                     DGTLAGRLREFSGQIQAKTGLLTYDNALSGFVTCSDGQTLAFSIISNDFVGKPRAVGL
                     IDRIVSVLAEHKNYPANAPTH"
     gene            1984..3108
                     /locus_tag="VFH91_00255"
     CDS             1984..3108
                     /locus_tag="VFH91_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6861433.1"
                     /translation="MFRMISKFCHTTLCLLALCVIASAQSNLTAKAGSQPNSSATIAE
                     TDRSRDGLVGPVRRVRTELAKLSTLDGKLAENKHVLVEVVAYDIKGNKVENQYFPIAG
                     ATLTGKEVYKYDEKGNISEMTMLNNDGSLLSKETYKYEYDFAGNWNKMTTSVAVVDGG
                     RLTFEPNEITYRSIMYYLDENMVKMVQPAANAPQPVSPAPTNVAGKSANDSKSQTNPV
                     AGAKQGENKTGSPTLPAASATANKLNNTIPAEVKPGDTDIAKTPVVDIGVEPPPTPGP
                     KPILKPVSGGVLNGTALSLPSPLYPDAAKRMRVAGVVTVEVILDETGKVVAANATTGP
                     TMLRESAVQAAMKARFSPTKLSGQPVKVSGVINYRFALAP"
     gene            3204..4136
                     /locus_tag="VFH91_00260"
     CDS             3204..4136
                     /locus_tag="VFH91_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET6861434.1"
                     /translation="MALTGHLSDLSLSELIEFFCNQRKSGRLKVLYPSGAGYFYLQSG
                     SVVDARIGVLHGIDAVYYALTLPNAEFEFSVDAEPGNRTINQPWTQVVLEGLRRLDEN
                     ISPGVAFPVGYVESGDGVNGESATSANGGVDGFSGPEFLSFSHPSSKRKPLFVGAIVV
                     AVLGSVAAIGVPAGWYHREKANASPTAEVSTPAPIQQTPTQPGEASSQPSLQTQTPVT
                     EVEDPAAVARRQREKERRAREAAKSAETSPGGAVPEAAPKSTAKRVVVTVTYDESGRV
                     TQASGGDANALRIARQKRFPPGKAGSATVTIPIN"
     gene            complement(4213..>5461)
                     /locus_tag="VFH91_00265"
     CDS             complement(4213..>5461)
                     /locus_tag="VFH91_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6861435.1"
                     /translation="MPSTLKLQTTPSVKELTSAPEVLEALAYFDANLEHITAEHIKIS
                     SVPASPFDERQRAEYLREKFVKLGLEEVTIDPEGNCLGLFSGNSSSPLLVVSAHLDTV
                     FSKDTDFSVHRRGNRLFGPGISDDASGLVALIAIIEALKTSQIKTRGSILFVATVGEE
                     GEGNLRGVRYLLTEGQWASRVDAFLSFDGPGIDRITNRALGSRRYRIEVTGSGGHSWG
                     DFGIPNPIHALGRAISRLAAYPIPKSARTTFNVGRIEGGTSVNAIPAFAAMEVDLRSA
                     VDAELRRIDAFFRRAVREAVDDENGGRRPGDQPLSLKLELTGERPTGTTPADSPLVEL
                     AMDATRAQGIEPKLDQSSTDSNLPISLGIPAITLGSGGNASGSHTLDEWYEPEGRALG
                     LKRGLLVILGVTGLSRSNRAQKN"
BASE COUNT         1294 a         1433 c         1432 g         1302 t
ORIGIN      
        1 acagtttcat tccggttggc gaacaacaca ggctcatttg tggtcccacg atccatcaca
       61 taaccgtcga acaagtctat accgtggcgc acgaggcact ggctaacagt cgaaccgaaa
      121 atctcttttc catcgctaac attgccgacg aataactttg gagtgcgacg gcctggcgtc
      181 gctttgtcat ctgtttacta acctccgtcc ctggttagcg acccaaaact cagtcgatcc
      241 ccaaatgcgt tacttaccgc gtccatttct cctggttctc ctgccgttgt tattagcctc
      301 ctgctcgtct gctccagaaa cgccgtcaaa ttactccgag ccgattgtcg ctgcttcctc
      361 gactccggct gtatttaatt ccgatgcaag tcttaccaag acactcgacg ccctgctcag
      421 tgaacccgaa ttcaatgtgt cgagactggg agttgcggtt atttcgttgc gagatggaaa
      481 gctgatttac caacgcaacg gagacaagtc atttacgcct gcatcaaata tgaaaatttt
      541 taccaccgcg gtagcactcg acttgctcgg cgcagattac cgttggcgta cgtcggtcta
      601 cgcaactcat gatgttgatt ccgcgggaac tgtaaatggc gatctggtcc tatacggacg
      661 aggcgcacca gatctggtaa cgagctccaa acccgataac aagaattccc tcgaagagct
      721 cgcgcggagt ttgtcgctcc gaggagtgcg gagggtcgca gggaatgtaa ttggtgacga
      781 gagctatttt cgtggcgagt caattggcga gggctggcag tggaatgatc ttcaatggta
      841 cttcggagct gaagcgagtg cgctcacggt gaactcaaat tctgtggacg taagcgtcga
      901 accacccggg aaggatgata accaaccgaa agttagctcg agtgatagcg acggctacgt
      961 aacaatcgaa aacaacatga agttggtgtc gcgcggcgac aagttcaccc tcggagttca
     1021 aaagggtgtg tcagacaaca atgtcaccgt ttggggcgag tttccctccg gcgcccgtgg
     1081 ctacggtgcg actctatcag tccatcgccc gtcgttgtgg gctgctcgaa tgttcatccg
     1141 tcagttgcaa gcggcaggca tttccgtaga cggctctgct gaatatcgcg acgccaaagt
     1201 gccgcaaagt caaagatttg atcctcacag cagcactgaa cttgccagcg tgactggaaa
     1261 gtcgttgggt gaggttgtcc acgcgacaaa taagctgagc attaacctat acgccgagtt
     1321 gcttttgcga acgctggggc acgaacgtgg tgccctgctg cagacggaac agcccgtggg
     1381 ccgcgaacag ggagacgagg aggctggaac agctttgatc atattttggc tcgctcgtgc
     1441 gggagttagc acaaacggcc ttgctcttca cgatggctcc ggtctctcgc gactggatct
     1501 cgttacgcct gaagcaacgg ccggcttgct ggccgcgata aggcgcacca cttctgccca
     1561 ggtgtttaca gattcgctcc caatcgcggg tactgatgga accctggctg gaagattacg
     1621 agagttttcc gggcagattc aggcgaagac cgggttgctg acgtacgaca acgcactttc
     1681 tggattcgtg acttgcagcg acggtcaaac attggctttc tcgattattt cgaatgattt
     1741 tgtgggaaaa ccgcgcgctg ttggcttgat tgaccgaatt gtttctgtcc tcgctgaaca
     1801 caaaaactac cccgctaacg ccccgaccca ttagttttcc taagttggct ctggacaaac
     1861 atggtttaac ggacttatta tggcaagggt ctgtcgggtg gctgtctgaa ttgccgtagt
     1921 gcccgcctca cttgttcgtc tttccaaaat tggcctgcct cagcgaattt tacggagaaa
     1981 accatgttta gaatgatcag caaattttgc cacactactc tttgtctcct tgctctttgt
     2041 gtcatcgcaa gtgctcaatc aaacctgaca gcaaaggcgg gaagccagcc gaattccagc
     2101 gcgactattg cggaaaccga tcgcagccgt gatggtttgg taggtcctgt ccgtcgcgtt
     2161 cggaccgagc tggctaaatt gtcaactctt gatggcaagc ttgctgaaaa caaacatgta
     2221 ctcgtcgagg tcgtggccta cgacatcaag ggcaacaaag ttgaaaatca atatttcccg
     2281 atcgcgggcg caacgctgac tggtaaagag gtttacaagt acgacgagaa gggcaacatc
     2341 agcgagatga cgatgctgaa caacgatggc tcgttgttga gcaaggaaac ttacaagtac
     2401 gagtatgatt ttgccggtaa ctggaacaag atgacgacct cggtcgcggt cgtggatggt
     2461 ggtcgactca cgtttgaacc aaatgagata acttatcggt ccattatgta ttacctggac
     2521 gagaacatgg ttaagatggt ccaaccggcg gccaatgctc cacagcctgt gagtcctgct
     2581 cccactaatg ttgcgggaaa atcagccaac gattcaaaga gtcaaacgaa ccctgtcgca
     2641 ggcgcaaaac agggtgagaa caaaaccgga tctccgacgc taccggcagc ttcggccacc
     2701 gcgaacaagc tgaataatac catcccagca gaggtaaaac cgggtgatac agatatcgcc
     2761 aagacgccag tagttgacat tggtgttgag cctccgccga ctcctggtcc aaagccgatc
     2821 ttgaagcctg tttccggagg cgttctgaat ggcacagctc tatcactgcc ttctccgctg
     2881 tatccggatg cggctaagag aatgagagta gccggcgtag ttactgtaga agtgattctg
     2941 gatgaaactg gtaaggtagt tgcggcaaac gccaccactg gaccaaccat gttacgcgag
     3001 tctgctgttc aagcagcaat gaaagcaaga ttctctccga caaaactttc cggtcaacca
     3061 gtcaaggttt caggcgttat caattacagg ttcgcgctgg ctccttgatc ccccgttact
     3121 agtggcgggc gcgtcgaaat tggcgcgctg ccattctctc catcttttcc aacctgtctt
     3181 ttgttagaag ggactaagtt ctcatggcac taactggaca cctcagtgac ctttcactat
     3241 cagagctaat tgagttcttt tgcaaccagc gaaaatccgg acgcttaaaa gttctatatc
     3301 catcaggtgc cggctacttc tatctgcagt ccggttccgt cgtggatgct cgcatcggtg
     3361 tattgcacgg aatcgacgcc gtttactatg ccctgacact cccgaatgcc gaattcgaat
     3421 tcagtgtgga tgctgagcct ggcaaccgaa caatcaatca accgtggacg caggttgttt
     3481 tagagggcct tcggcgcctt gacgaaaaca tttctccggg agttgcattt ccggttggat
     3541 acgttgagtc gggcgatggt gtgaatggcg agtcggcgac ttccgctaat ggcggagttg
     3601 acgggttttc cggtccagag tttctttcct ttagccaccc gtcgtcgaaa cgtaaaccac
     3661 tttttgtggg agcaatagtt gttgctgttt taggtagcgt tgccgcgatt ggagtgcccg
     3721 caggttggta ccacagagaa aaggcaaacg cttccccaac agcagaggta agcacaccgg
     3781 ctccaattca acagactcca acccagccag gagaagccag tagccaacct tcccttcaga
     3841 cgcaaacccc tgtaaccgaa gtcgaggatc ccgcggccgt ggcgcgaaga caacgtgaaa
     3901 aggaacgacg ggcgcgtgaa gcggcgaaga gcgcagaaac atcaccaggt ggtgcggtgc
     3961 ctgaagcagc acccaaatca acagcgaaga gagttgtcgt gaccgttacc tatgatgagt
     4021 cagggcgtgt cactcaagcg tcgggtggtg atgcgaatgc gttacgaatt gcgcgtcaaa
     4081 agcgttttcc gccaggcaaa gccggctctg ccaccgtgac cattcctatc aactaaaaat
     4141 atctctgcga gatcactgca ttgctttatg tttgccactg atttcaacgc agagtgacgc
     4201 agagtatttg cgttagttct tttgtgctcg gttacttcgg ctcaagcccg tcacgccaag
     4261 gattacaaga agacctcgtt taaggcccaa tgcgcgacct tctggctcat accattcatc
     4321 gagggtatgt gagcccgaag cattgccgcc gctgccaagg gttatcgcag gaattcccag
     4381 tgagattggt aaattggaat ccgtagacga ctggtccaat tttggttcga tgccttgagc
     4441 tctcgttgcg tccatcgcca gctcaactag cggagagtca gcaggagttg ttccggtcgg
     4501 acgttcaccg gtgagttcca gtttaaggga tagaggttga tcacccggtc tccgtccgcc
     4561 attttcgtcg tcaaccgctt cgcgtactgc ccgccggaaa aaagcatcaa tgcgacgcaa
     4621 ctcggcgtca actgccgacc gtaggtccac ttccatcgca gcgaaagcag gtatcgcgtt
     4681 cacgctcgtt ccgccttcta ttcgcccaac attgaatgtg gttcgagcac ttttcggaat
     4741 gggataagca gctagtctgg agattgcgcg accaagtgca tgtattggat taggaatgcc
     4801 gaaatctccc caggaatgcc cgcccgatcc ggtaacttct atgcgatacc gtcttgatcc
     4861 gagtgctcga ttggtgatgc gatctattcc tggtccatca aaactgagga acgcatcgac
     4921 gcgactcgcc cattgacctt ccgtcaagag gtagcggacg cctcgaagat ttccttctcc
     4981 ttcttcgcca accgtcgcca caaacaagat agaaccacgt gtttttattt gagaggtctt
     5041 taaggcttct ataatcgcta tcaatgcgac aagaccggac gcgtcgtcag agatgcctgg
     5101 cccaaacaat cgattgcccc ggcgatgcac actgaagtca gtgtcctttg agaaaactgt
     5161 atcgagatga gcactcacga cgaggagcgg cgacgacgag ttgccggaaa aaagccccag
     5221 acaattgcct tccggatcga tcgtcacttc ttccaggccc agcttaacga acttctcccg
     5281 taaatactcg gctcgttgtc tttcgtcgaa cggacttgcc ggcacactgc tgattttgat
     5341 gtgctccgcg gttatgtgtt caaggttagc atcgaagtag gcgagagctt ctaaaacctc
     5401 cggcgcactg gtaagttcct tgacgcttgg agttgtctgt agttttaagg ttgaaggcat
     5461 g
//
LOCUS       DASSFP010000012         3320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1044486, whole genome shotgun sequence.
ACCESSION   DASSFP010000012 DASSFP010000000
VERSION     DASSFP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044486"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFH91_00270"
     CDS             <1..737
                     /locus_tag="VFH91_00270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET6861436.1"
                     /translation="PGGSGLASDIIWHAVDAANQKKPVVVSMSDVAASGGYYISASAA
                     KIVAQPSTITGSIGVVAGKPVMRGFYDWLGISNEYVLRGKTAGMFRETEKFSDDERAK
                     FEDWIKTTYYQDFVPKVAKGRKKDAQYVDSVGQGRVWTGFQAKERGLVDEFGGLDRAI
                     DVAKELAKIPADKGVQRVIFPYPTTFLQQLLSGNENSNTKVQQQEAVVAALPEDARRA
                     FRYMAMMERMKNGDTMLIMPFELRVR"
     gene            complement(917..2200)
                     /gene="serS"
                     /locus_tag="VFH91_00275"
     CDS             complement(917..2200)
                     /gene="serS"
                     /locus_tag="VFH91_00275"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6861437.1"
                     /translation="MLDLNYVRENLDIVRAALKARKGEIQALDDFAEADAQRRRVIAE
                     SDQLNAQRNASSREIGALMKEGKNEEAEKRRADVGKLKEQIASLDHLRTETEAKMQQL
                     LSTLPNIPHTSVPVGKDESDNVEVRRWGSAPSFSFEPKDHVDLGGALGILDLERATKI
                     AASRFAILNGAGARLERALINFMLDVHTREHGYLETLPPFIVNRSSLFGTGQLPKFEP
                     DLFKLEDDRELYLVPTAEVPVTNYYREEILDASDLPMRWVAYTPCFRSEAGSYGRDTR
                     GVIRQHQFEKVELVKYALPENSYEELEALTHDAEVILQKLGLHYRVVTLSTADLGFGS
                     AKTYDIEVWLPSQNMFREISSCSNYEAYQARRAQIRFRRAGGSKPEFVHTLNGSGLAV
                     GRTWIAIVENYQQEDGSILIPEVLRPYVGFDRIGP"
     gene            2692..>3320
                     /locus_tag="VFH91_00280"
     CDS             2692..>3320
                     /locus_tag="VFH91_00280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET6861438.1"
                     /translation="MSEHKTFSGRAAIVTGATRGIGRAIALELARRGADVAFNYAKSA
                     ESAESLKSELESHGVRALAFQCDVGQTEAAANMVNEVKTAFGRIDFLVNNAGVTRDNL
                     ILRMKESDWDEVIDTNLKGAWNFAKAALRTMLRQETGGSILNISSISGEVGMPGQSNY
                     SASKAGMIGLTKSLAKEVASRKVTVNALALGMVATDMAGKLDQSYQDKI"
BASE COUNT          846 a          826 c          874 g          774 t
ORIGIN      
        1 gtccaggcgg ctcggggttg gcctcggaca tcatctggca tgcggttgat gctgccaatc
       61 aaaagaaacc agttgtggtt tcgatgagtg acgttgccgc ctcaggtggt tattacatct
      121 cggcgagcgc cgcaaaaatt gtggcccaac cgtcaactat tacaggatcg attggcgtag
      181 ttgccggcaa accagtgatg cgtggttttt acgattggct cggcatctcg aatgagtatg
      241 tgttgcgtgg caaaaccgcc ggaatgttca gggagactga aaagttttcg gatgatgagc
      301 gggcaaagtt cgaagattgg attaagacga cgtattacca ggactttgtt ccgaaagtcg
      361 cgaagggccg aaagaaagat gctcaatatg tagattcagt cggtcaaggc cgagtctgga
      421 ccggatttca ggctaaggag cgcgggctgg ttgatgagtt cggcggactt gatcgcgcca
      481 tcgatgtcgc aaaagagctt gcgaagatac ccgctgataa gggagttcag cgagtaatct
      541 ttccttatcc aactacgttc ctgcagcaat tattgagtgg caacgagaac tcgaatacga
      601 aagtacaaca acaagaggct gtagttgcag cattgcctga agatgcgcgc cgggctttcc
      661 gctacatggc aatgatggag cgaatgaaga acggggatac gatgcttata atgccttttg
      721 agctgagagt tagataaacg gcctcgcggg ctcatcggtt ctacggtgga accactaacg
      781 ttaccgtaga gccgcagcgc agcatcgccg ccaccaaaaa ctgagtagta acttgactta
      841 ctctgtgcaa cctttgtgtc ctaagaagac cgaagagtct cagaggcagc acagagaagc
      901 ttcagattag ctgtagctaa ggaccaatcc tgtcaaagcc cacgtaaggc ctaagcactt
      961 ccggaatcag gatcgagcca tcttcctgct gataattctc cactatcgcg atccatgttc
     1021 gtccgactgc cagtcccgat ccgtttaatg tgtgaacgaa ctccggtttg gagccacctg
     1081 cgcgacgaaa tctaatttgt gcccgtcgcg cctgatatgc ctcgtagttt gaacaggacg
     1141 agatctcgcg gaacatgttc tgagagggca accaaacttc aatgtcgtat gtcttggctg
     1201 agccaaatcc cagatccgca gttgagagag tcaccactcg ataatgcaaa ccaagcttct
     1261 ggagaattac ttctgcatcg tgcgttaatg cctcgagctc ctcatatgaa ttctcaggaa
     1321 gcgcatactt aaccaactca accttttcga actgatgctg gcgaatcact ccgcgagtat
     1381 cacgtccata actaccggct tcagaacgaa agcatggagt gtacgcgacc caacgcatcg
     1441 gtaagtcaga agcgtcgaga atctcttcgc gataatagtt agtaaccgga acctctgcag
     1501 ttggcacaag gtaaagttca cgatcgtctt caagcttgaa gagatcgggt tcgaacttcg
     1561 gcaactgacc ggtgccaaac agcgacgacc gattaacgat gaaaggtggc aacgtctcga
     1621 ggtagccgtg ctcacgtgta tgcacgtcaa gcatgaagtt gataagtgct cgttcgagtc
     1681 gcgcgccggc gccgttcaga attgcgaagc gagaagcagc gatttttgtc gcccgttcaa
     1741 gatcgagaat gcctaatgca ccaccgagat cgacgtgatc cttcggttcg aaactgaatg
     1801 acggcgcaga accccaacgt cgaacctcga cattgtccga ttcatctttg ccaactggta
     1861 cgctcgtgtg agggatatta ggcaaagttg agagcagttg ctgcatcttc gcttcagttt
     1921 ctgttcgcag atggtccaac gaggcaattt gctctttaag ctttccaacg tccgcacgtc
     1981 gtttctcggc ttcctcgttc ttgccttcct tcatcagcgc gccgatttct cgactggaag
     2041 catttcgctg ggcattgagc tggtccgact ctgcaatcac tctgcggcgc tgagcatcag
     2101 cttccgcaaa atcatcgagg gcctgaattt cgcctttgcg cgcctttaat gcagcgcgca
     2161 ctatgtccaa attttctcta acgtaattga gatcgagcat taacagactt tgcaactggt
     2221 gcggtcagcg tgtcaactgg gggaaaggct ggcctttggc cttcggcctt tggtcttcgg
     2281 tctttggtct ttgcacaaag taccaaggtc tcaggccaaa gacccaaggc caaaggccaa
     2341 agaccaaaca ccaaacaccc atccttccat ttgttctcct ccgaccgtat atgttattag
     2401 agaccgcctt gctcagctaa acccaggaac ggacggacgg aacattgaca gcgaaacatc
     2461 attgttgcgg aacgatggaa acagaagtga atcgcaaatg ctctgaacat tcagacccgt
     2521 ttgattgccc ggatcacaca gtctattact ccgctgagtt caaggaatac ggcatactcg
     2581 ttcacgatgg tggctcgtca tactccgtca tccgctactg cccctggtgc ggcagcactt
     2641 tacctatatc aaatcgagta accggaacat tggaaggaag gaccaccgaa agtgtctgag
     2701 cacaaaacat tctccggacg ggcggcaatt gtcaccggtg ctacccgtgg aataggacgc
     2761 gcgattgctc tcgagctagc tcgtcgaggc gcggacgtcg ccttcaatta tgcgaagagc
     2821 gccgagtccg ctgaaagtct taagagcgaa ttggagagtc atggagtgcg agcactcgca
     2881 tttcaatgtg acgtcggaca aacggaagcg gcggcgaata tggtcaacga agttaagact
     2941 gctttcggtc gaatcgactt tctcgtcaac aatgccgggg tcacccgcga caacctcatt
     3001 ctcagaatga aggagagtga ttgggacgaa gttatcgaca ccaatctcaa aggcgcctgg
     3061 aattttgcga aggcagcact gcgtacaatg ttacgtcagg aaactggcgg gtcgattctt
     3121 aacatttcgt caatcagtgg tgaggttgga atgcctggcc agtcaaacta ctctgcgtca
     3181 aaagccggta tgattggtct gacgaagtcg ttggcaaagg aagtcgcaag cagaaaagtg
     3241 acagttaacg cgctcgcgct tggtatggtc gctacggata tggcgggaaa gttggatcaa
     3301 agttatcagg acaagattaa
//
LOCUS       DASSFP010000013         4685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2646, whole genome shotgun sequence.
ACCESSION   DASSFP010000013 DASSFP010000000
VERSION     DASSFP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="VFH91_00285"
     CDS             complement(<1..1590)
                     /locus_tag="VFH91_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861439.1"
                     /translation="MICKSCYEKRRTSKGRCTATGCRGSIYNKKLQLCRYHNDERFAP
                     KRLDKYIKSYDSPFPQNVRYFLALTTKLGLANCNVDERSVLVRDLLKYRAVGEYLQSH
                     ELPEMLTWQAIHEALPKARKRGQRGRAKVHFIRSGLLELGHHFLPEQLVPYQRERLLE
                     KWLRSAPAMFVEHVTAFEKWLSKGMLNPKLNIYLHEAQPLANTSDYTRKTIETVIKFL
                     EWCVKCDICSLANITESTAASYKETLFWQLECSACRKRIPLDVAKTKEICSNQECQAS
                     NSYVKIRRLARASVNQYTVNLRTFFNWAQLHDVVAENPFPYNTEKIRRSFTVMNKRGQ
                     MVEISASIRRYDDKVVERFFSYMASPDADPEEALILYLIFFHLLTVTEMRNAKIPSLV
                     APENPTGECDRATDFEYLLLPVRQPSRGLQSPRREGPIMKYAKKAAWWLRPLLERYFE
                     KRKLIGVSEYLFVGRYQLTQNRPVCDTQILDLLQRASQRVLNGMVNPRDLRNTAAAIR
                     ADRSKRRGAILTRLGYTRGWAT"
     gene            complement(1960..2331)
                     /locus_tag="VFH91_00290"
     CDS             complement(1960..2331)
                     /locus_tag="VFH91_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6861440.1"
                     /translation="MPTNWKLRELLKERGVKSASEISRSLQEVGYQLSVQAVCDLLNR
                     PPKMIRLETIEAICNAFYIPLSEFLEVLPMASQKPQKRMRTLQSAQRVQKRGVGPSIG
                     TIDYAGFYPDARKFSSGSTKV"
     gene            complement(2331..3488)
                     /locus_tag="VFH91_00295"
     CDS             complement(2331..3488)
                     /locus_tag="VFH91_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6861441.1"
                     /translation="MELPNPISPRVQQPPIILWEEQLHDHNQKIEEYLQNRVFRGCTY
                     ESTVKSARTVLKRLFDLVPIPDPNHPDGHRHILVWEFLDPVRGSSRFGVLMSSLLQGN
                     LAYGTRRRYLTELRAFCDYVVAKPNIPGSSNLTIIEKYGPIALPFTKYDLPVHAQDRP
                     TKPRYALSAEVRDQFYESLRTEYLPNHPVPHLGAQDYTAIVLQTEIGARTSELLGIRS
                     SGASCDIDWQAGRVRLFGKAKAFSGKRVRTVPLTPFAMEVLRVFEKIFKPMFPRRRDE
                     SEYLFLDKDGDPLMDHQYIYNFRKIVELARDAGVPLPEDLCPHDLRRTCATLALEQNP
                     LEYRKVLRKLGHTYPSSAAPYLIATDADVEDEQGDLIDIFIDPDMEKRGTK"
BASE COUNT         1087 a         1176 c         1185 g         1237 t
ORIGIN      
        1 agtggcccat ccacgtgtgt aaccaagccg tgtgagaatc gcgccgcggc gcttggatct
       61 gtccgcccga attgcggctg cagtgttacg taggtcacgc gggttaacca ttccattcaa
      121 tacacgctgt gaagctcgtt gtagcagatc gaggatttgt gtgtcgcata ctggcctgtt
      181 ttgtgtcagc tgatagcgcc caacaaatag atattcagag acaccgataa gttttcgctt
      241 ctcgaaataa cgctctaaca gaggacggag ccaccacgct gccttcttcg catacttcat
      301 tattggtccc tcacgtctcg gtgactgtag cccccgactc ggttggcgaa cgggtagcag
      361 caaatactcg aaatctgttg cgcggtcgca ttcgcctgtt ggattctccg gcgccaccaa
      421 cgacggtatt tttgcgttgc gcatctctgt cacagtaagc aggtgaaaga agatcaaata
      481 caatatcaac gcctcttcgg ggtcagcgtc aggcgaagcc atataactaa agaagcgttc
      541 gacaacttta tcgtcgtacc gcctgataga agctgagatc tcaaccatct gcccacgttt
      601 attcatcacc gtgaagcttc ttcggatctt ctcggtgtta taaggaaacg gattttccgc
      661 gactacatca tgtagctgcg cccaattaaa gaacgtacga agattcactg tgtattgatt
      721 aactgaggca cgagccaacc ttctgatttt tacgtaagaa ttgctcgcct ggcattcctg
      781 attactacaa atttcttttg tcttagcgac gtctaacggg attcgttttc ggcacgcgct
      841 gcactccagt tgccagaaaa gagtctcttt gtaacttgct gccgtgctct cggtgatgtt
      901 ggcgagtgag cagatgtcgc acttgacgca ccactcaagg aacttgataa cagtctcaat
      961 agttttgcgt gtgtagtcac ttgtattggc aagaggctgc gcttcatgca ggtatatgtt
     1021 gagcttcgga ttcaacatcc ctttcgatag ccacttctcg aaggcagtta cgtgctcaac
     1081 gaacatcgcc ggcgctgacc tcagccactt ttccaatagg cgttcacgtt ggtacggcac
     1141 cagctgctcc ggcaggaaat ggtgacccag ctctaataag cccgagcgaa tgaagtggac
     1201 cttggctcgt cctcgttgcc cgcgtttcct ggctttcggc aaagcttcgt gaattgcctg
     1261 ccaggtcagc atttcaggaa gctcgtgact ctggagatat tcaccgaccg cgcgatactt
     1321 aagtagatct ctgaccagca cgctcctttc gtcgacattg cagttagcca atcctaactt
     1381 cgtggttaga gccaagaaat agcggacgtt ctgcggaaac ggcgagtcgt agctcttgat
     1441 gtacttatcc agtcgttttg gggcgaagcg ctcgtcattg tgatatctgc agagctgcaa
     1501 ttttttgttg tagatggacc ctctacatcc ggtggccgtg catcttcctt tgctggtgcg
     1561 acgcttctcg tagcacgatt tacagatcat ttccgcgttc ggaagagagg tcttcgtgag
     1621 cccacaagcg caacatacga acttccgaag gcgcaacttc agattttttc tatggatcgc
     1681 attgaggtaa cacttgccac aatggccggg gtagctgata cgaggataat cggccagccc
     1741 acacttttca caagcaatct ttaccttaga cagtctttta gagcacggca gacaaaatgg
     1801 agagtctggt tgtagcctgc ggacttttgt tggacaccca ccgcattgca ctttcggaag
     1861 cttgcgtaca cacaggtcgc atacatcgaa ccaccgtctg ctttttcgcg gcaacttaca
     1921 ctcttcacag attatagaac gtcctctttg catctttctt cacacctttg ttgaccctga
     1981 tgaaaacttc cgcgcatccg gatagaagcc ggcataatct attgttccga tacttggacc
     2041 aaccccgcgc ttctgtactc gctgcgcact ttgtagagtt cgcatccttt tttgaggctt
     2101 ctgcgatgcc atcggtaaca cttccaggaa ctcgctcagc ggaatgtaga acgcgttaca
     2161 gattgcctcg atcgtttcga ggcggatcat ctttggcggg cgattcagga ggtcgcatac
     2221 cgcctgtacc gataattggt agccgacctc ctgaaggctt cggctgattt ccgatgcgct
     2281 cttgactcca cgctctttca acaactctcg caacttccaa ttagtaggca ttactttgtt
     2341 cctcgtttct ccatgtctgg atcgataaaa atgtcgatga gatcgccctg ttcatcctca
     2401 acatcggcgt ccgttgcaat gaggtaaggc gcggccgagc tgggatacgt gtggcccagt
     2461 tttcttagaa cctttcggta ttccaatgga ttttgttcga gcgcaagtgt tgcgcaggta
     2521 cgtcgcaggt cgtgagggca aaggtcttcc ggtaggggca ctccagcgtc tcgagctagt
     2581 tcaacgattt tcctaaaatt gtagatgtat tggtggtcca tcagcgggtc tccatctttg
     2641 tctagaaaga gatattcaga ctcatcacgc cgccgcggga acattggttt gaagattttc
     2701 tcaaagacac gcaacacctc cattgcaaac ggcgtgagcg gaacggttcg cacacgctta
     2761 ccgctaaatg cttttgcttt gccaaagaga cgcacacggc ccgcctgcca atctatgtcg
     2821 caacttgctc cactcgaccg tattcccaga agttcactgg tacgcgcgcc gatctcggtt
     2881 tgtagaacaa tggccgtata gtcctgagcc cccagatgag gcactggatg gtttggcagg
     2941 tattcggtgc gcagggactc atagaactgg tcacgcactt ctgcggataa ggcgtaacga
     3001 ggtttggtgg gcctgtcctg cgcatgcact ggtaaatcgt atttggtaaa cgggagtgcg
     3061 atcggaccat acttttcaat aatggtgagg ttgctgctgc ccgggatatt aggcttagcg
     3121 acgacgtagt cgcagaacgc tcgcaactcg gtgagatacc tgcgcctggt tccgtaggcc
     3181 agattgccct gtaacaacga tgacatcagt acaccgaacc gcgacgatcc gcgcaccgga
     3241 tccagaaact cccacacgag tatgtggcga tgtccatccg ggtgattagg atccgggatc
     3301 ggtaccagat cgaagagacg cttcagtaca gttctcgctg acttcactgt tgattcgtag
     3361 gtgcaaccgc ggaagacgcg attctggaga tactcctcga tcttctgatt gtgatcgtgg
     3421 agttgttctt cccacaagat gatgggtggt tgctgtacgc ggggtgatat cgggtttggg
     3481 agttccatag taaacctttc gtttaagatt tggttgctct atcaagcgcc tctttaatat
     3541 tccagcgctt tttgattctt ttgttggccc atcttcctgt ctaagttgat ggaccagaaa
     3601 cagttctgct gaaaacaaat ggaaactcgc ttctcaaagt ttccagtgga atgttttctg
     3661 ggttcgatgt ttcaaagtat gcgaatccag tgtttggacc gcacttgttc ttggtgagta
     3721 ggggcgtaga tcaaaagacc tgatcagatc gtctacaaaa tgtgtgccat ggacaccgtt
     3781 cgtgaagcga ggtgctgtta gtcagttttc ttgcaagggc aattcgtgag gtggtccatg
     3841 tgtctccact caaaataaaa ccaacttcta caaagagatc cgtgctggta gtgacgacca
     3901 ttcatatcgt gcgagccatt ctctaaggag agtcaaagtg tcagatagag gcttccgatt
     3961 ttgcgttggc ctctcaaaat gagcccgagg ggtgttagcg atcgcgaaca ccccaacaat
     4021 tcggttgcgc acacttaggc gcttaaacca atgattgaga ggcacttcaa actaatccga
     4081 ggggtgtctg cgatcgcgaa caccccggta ttcgatcgcg cacacctaag ggcttaaacc
     4141 aatgatttgg gaagtaagaa agagcgttcg gctattgcta ttttatggtc caaggtaaga
     4201 tttctggccg cttttcttaa taaaccaaac caaacacaat cgctaagccc attagtcttt
     4261 aggcccatct caacacggca cgcgatgtgc ccgtaaaaaa attgacgcgt tgagcgtagg
     4321 tactcgccta atgactaaga gcaattgttg agccagtggc ggggcgaaaa attttgacat
     4381 tgcgtgagcg aagtcttaaa ccggcgtgaa gggtctagat cgacgccgaa gcgttcgtat
     4441 atgcgaacgc aatggttcgt ttatggaaac gcaatggttc gcatttgcga acctcagcga
     4501 ttaataatga agcggagagc ggctaggggc tacggagaag gacgaaccgc tgaggacaac
     4561 ttgtctattg gccggcggca tttgacatcg gtttgcgaag ccatagttcg gatatccgga
     4621 cactctttaa ggtcgaaagt cgggatatcc gaactttgcc ttgcccaatg gttcgccccg
     4681 tcgac
//
LOCUS       DASSFP010000014         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_448350, whole genome shotgun sequence.
ACCESSION   DASSFP010000014 DASSFP010000000
VERSION     DASSFP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448350"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFH91_00300"
     CDS             <1..909
                     /locus_tag="VFH91_00300"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET6861442.1"
                     /translation="NVDFITRNDPGKWRQDPISLNPLALGSHWGDVSPFVVESSSQFR
                     APAPPAMTSAAYANAFAEVKRLGGDGIITPTERTADQTIIGIYWAYDGTPSLCAPPRL
                     YNQIAMDIAETRNTNKLIDVARLLALINTAMADAGTTVWESKYFYQFWRPVIGIRESD
                     PGTGPTGLGDGNAATIGDITYSPLGAPASNLTGPNFTPPFPAYPSGHAGFGGALFQVL
                     RRFYCTDRIKFSFISDEFNGVTEDNGGNPRPLIERTFNTLSQAEEENGQSRIYLGIHW
                     SFDKTEGIAQGRRIGDYVFDHAFGKK"
     gene            1175..1957
                     /locus_tag="VFH91_00305"
     CDS             1175..1957
                     /locus_tag="VFH91_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET6861443.1"
                     /translation="MAARAIWKGALKLGTTKVSVKLYSAIEDKTVRFHILDSGRKRVK
                     QHMVEPDTGADVPDAEIRKGYEVKPGRFVILEESDLESLKPKASRDIELSSFVPIDAI
                     EQQYYERPYYLGPDGDEKNYFALAEALENQEREGIAHWTMRNKTYTGALGAQDGYLVL
                     STLRNAEEVVSAAQLPKPAGTAPSKKEVELAKHLVSMLEGEFDPAEYKNEYRERVMDF
                     IAKKAKGHAPRLSAVKTKRQTTSLDSALAKSIESLKKGKRAA"
     gene            2019..2819
                     /locus_tag="VFH91_00310"
     CDS             2019..2819
                     /locus_tag="VFH91_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET6861444.1"
                     /translation="MSSDEDGGGGVRPFWSGTITFGLVSVPVNLYPATRTNRTPLRMI
                     GPEGEPLSRKYYSEKTGKDLDADDMIRGYEIDKGKFVTVTDEELERLAPDKTRDINLT
                     LFVKEESIPPVYFERGYFLSPAGNSEKAYKLLAEAMKERGDVGIATFVMRGKEYPVAI
                     FSDNGILRAETMRFPDEIRSAKQVGLPERPKTNATTVRKFETLIKKKTKRTFTPDKDE
                     ETAQLLKLVKKKEANKSNVVQIKESDASEGKVIDLVEVLKRTLAGKAK"
     gene            complement(2975..>4916)
                     /locus_tag="VFH91_00315"
     CDS             complement(2975..>4916)
                     /locus_tag="VFH91_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6861445.1"
                     /translation="VTLNDQFLGEYRGGYTPFSFEVTEHLNWRGNNLLAVEVDSTERN
                     DIPPFGGEIDYLTFGGIYRDVALRFVNSAFIDNVFAKPVDVLKDTRRLEVRCFINSKS
                     GASRALKVELFDGPKVISSATQNISSANPFQDITLQNLAGVQLWDIATPKLYLVRVTL
                     MEGETSVDSYETRIGFREAKFTPEGFFLNGKHLKLRGLNRHQTYPFVGQAMPARVQRR
                     DAWILRKELKCNIVRTSHYPQSPHFLDACDELGLMVLEEIPGWQHVGDKAWQELSVHY
                     VDAMIRRDWNHPSIIIWGVRVNESGDYHDFYTQTNKLARSLDDSRPTGGVRYKYDSEL
                     LEDVFTMNDFQIPLRRPNHPLYLNTEFIGHMYPTKRHDNVERVTEHAMRHARVHNQLA
                     SDKAYAGGIGWCAFDYNTHANFCSTDRVCYHGVADIFRIAKPAGGFYKSQCDPKEEIV
                     LEPAFDWARGDRNESFTTAMVSSNCDEVKIYISDRLVAELKPDRETFPHLQYAPFITN
                     LRDAFRKGWGDLKLEGYIDGKKVIEKQLSGRGADRQLLVQADDTELTGDGIDATRVVF
                     RVTDEFGSGRPLANAALQLSLTGPGEIIGENPFALFGGVGAVWIKTKEATGVIRLTVA
                     HPTLGNKTVTIKAVAPKTRPII"
BASE COUNT         1292 a         1209 c         1264 g         1151 t
ORIGIN      
        1 aacgtcgatt tcatcactcg taatgatccg ggaaagtggc gccaggaccc aataagtctt
       61 aacccgctcg cgcttggatc gcattggggc gacgtcagtc cgtttgtggt cgaatcatcc
      121 agccaattcc gcgctccggc acctcccgct atgacaagtg ctgcctatgc caacgccttt
      181 gcggaagtaa agcgactggg cggcgatggc attatcactc cgactgagcg tactgccgat
      241 caaacaatca tcggaatcta ctgggcctac gatggtacgc cgagtctgtg cgctcctccg
      301 cggctctaca atcagattgc gatggatatt gctgaaacaa gaaacaccaa taagttgatc
      361 gacgtcgcac gattgctggc gttgattaac acagcaatgg ctgacgccgg aacaaccgtc
      421 tgggaatcga aatacttcta ccagttttgg cgaccagtga ttggaattcg tgaatcagat
      481 ccaggtactg gaccaaccgg tctaggagac ggcaacgcgg ccaccattgg cgacataact
      541 tattcgccac tcggcgcgcc ggccagcaat ctgactggac caaactttac gcctcccttc
      601 cccgcctacc catcaggaca tgccggattc ggtggagcgt tgttccaggt gttacgtcgc
      661 ttctactgca ccgatcgaat caagttctcg tttatctcgg atgagttcaa tggcgttacc
      721 gaagacaatg gcgggaatcc aaggccgttg attgaacgaa ctttcaatac tctttctcaa
      781 gcagaggagg agaacggtca aagtcgaatc tacctgggaa tacattggag cttcgacaag
      841 actgaaggca ttgcccaggg aaggcgtatt ggtgactacg tcttcgatca tgcctttggg
      901 aagaagtaag tttgacgtta cggatgataa ggttttctgg aactcaacgt gcaaagggaa
      961 atctttgaag atgggtaagg ccaaaacttt aacgccgtgg tggcttgaaa ccggtacaga
     1021 gatctgttcg atctgccatc aggtgtacat ttacgagacg gagtgccggt gcgttgattg
     1081 tgatgctggc gtctgttccg actgcgtgac tatcgagtcg accgcaataa tttgcatacc
     1141 ttgctcaact tcggaagagg agggttgacc ttcaatggcg gcaagagcaa tttggaaagg
     1201 tgccttaaaa cttgggacaa ccaaagtctc agtcaaattg tactccgcga tcgaggacaa
     1261 gactgtcagg ttccacatcc tcgacagtgg ccgtaaacgc gtcaaacaac acatggtcga
     1321 accagatacc ggtgcggatg ttccagacgc cgaaatcaga aaaggctatg aggtcaagcc
     1381 cggaagattt gtaatccttg aggaatcaga tctcgaatcg ctgaagccaa aagcctctcg
     1441 agatattgaa ctttcatcct tcgttccaat cgacgccata gagcaacagt attacgagcg
     1501 tccttactat cttggtccag atggggatga gaagaactac ttcgccctgg ccgaagcgct
     1561 ggagaatcaa gaacgcgaag gcatcgctca ctggaccatg aggaacaaaa cttatacggg
     1621 tgcgctcgga gcacaagatg gctatctcgt cttgtccacg cttagaaatg cggaggaggt
     1681 agtgtcggct gcgcaattac caaaaccagc agggacagca ccgagcaaaa aagaggttga
     1741 gcttgctaaa catcttgtga gtatgctgga aggcgagttt gatccggccg agtacaagaa
     1801 tgagtatcgc gaacgcgtaa tggacttcat cgcgaagaag gccaagggac atgcgccgcg
     1861 cctcagtgcg gtgaagacca aacgccagac tacatcgctc gacagtgccc tggcgaagag
     1921 cattgagtcg ttgaagaaag gaaaacgagc tgcctaaaag aatttcaaaa aagagttcga
     1981 ccgctgctca ctccgctacg cgtcggcata gtagtggtgt gagctcggat gaggatggcg
     2041 gcggcggcgt tcgaccgttc tggtcgggca ccatcacgtt tggcctcgtt agtgttccgg
     2101 tcaatcttta tccggctacg cgaactaacc gcacgccgtt acgaatgatt gggccagagg
     2161 gcgaacctct gtcgcgcaag tactattctg agaagacggg gaaagatctc gatgctgacg
     2221 acatgatccg cggttatgag attgataaag gaaaatttgt aaccgttact gacgaagaac
     2281 tcgagcgatt agcgccagat aagacgcgcg atattaatct gactctcttc gtcaaggagg
     2341 agtcgattcc gccggtgtat ttcgagcgcg ggtattttct gagtccggcg ggaaattccg
     2401 aaaaagcata caagcttctg gctgaagcga tgaaagagcg gggagacgtc gggattgcta
     2461 ccttcgtgat gcgtggtaag gaatatccgg tggcaatctt ttctgacaac ggaattctac
     2521 gcgctgagac aatgcgtttt ccggacgaaa ttcgatccgc caaacaggtg ggcctaccgg
     2581 aaaggcctaa aacgaatgca acgacagttc gaaagtttga aacgctgata aagaagaaga
     2641 caaaacgaac gtttacgcca gacaaggacg aagagactgc tcagctccta aaactcgtta
     2701 agaaaaagga agctaacaaa agcaatgtcg ttcaaataaa agaatctgac gcatctgaag
     2761 gcaaggttat cgatttggtg gaagtactta agcgaacgct ggctggcaag gccaagtaac
     2821 acgttgtatg cgcgtctgca ggaagcgatc atttttcatt ttccattttt catttttgag
     2881 ctcatttagg tctcacccaa atgtaaatta caggtcgact cagttataaa atgagagatg
     2941 aaaaaggaaa tgacgaatag aaaattatcg cctttcaaat gatgggccga gtctttggtg
     3001 caaccgcctt tatcgtaacc gtcttgttcc cgagcgtggg gtgagcgacc gttagccgga
     3061 ttacccctgt agcttccttt gtcttgatcc agaccgcgcc aacgcctcca aataacgcaa
     3121 agggattttc gccgattatc tctcctggac cagtgagtga taactggaga gcagcgtttg
     3181 ccagtggacg tcctgagcca aattcgtcgg tcactctgaa gacaacgcgc gtcgcgtcga
     3241 ttccgtcgcc tgtcagctcc gtatcgtccg cctggaccaa caactgtcga tcggcgcctc
     3301 gtccagacag ctgcttctcg atcaccttct taccatcgat gtaaccttcc agcttcaagt
     3361 cgccccagcc tttgcgaaac gcgtcgcgca ggttagtaat aaatggcgcg tactgcagat
     3421 gcggaaaggt ttcccggtca ggctttaact cagcaaccaa acggtctgag atatagattt
     3481 tcacctcatc gcagttagac gaaaccatgg cggtcgtaaa gctctcattg cgatcgccac
     3541 gggcccaatc aaatgcgggc tccaacacaa tttcttcttt cggatcgcac tgagacttgt
     3601 agaaacctcc ggctggtttc gcaatccgaa agatgtcagc cacgccgtga tagcaaacac
     3661 gatcggtcga acaaaaattc gcgtgggtgt tgtagtcgaa cgcacaccaa ccgataccac
     3721 cggcatatgc cttatccgaa gccaattgat tatggacccg cgcatgacgc atcgcgtgtt
     3781 cggtaacacg ttcaacgtta tcgtgccgct tggttggata catgtgtccg ataaactcgg
     3841 tatttagata gagaggatga tttgggcgtc gcaacggaat ctggaaatcg ttcatcgtaa
     3901 agacgtcttc cagtaactct gaatcatact tataacgcac acctcccgtt ggcctcgagt
     3961 cgtccaggct gcgggccaac ttattcgttt gcgtgtagaa gtcgtgatag tcacctgatt
     4021 cgttgacgcg cacaccccag atgatgattg acggatgatt ccagtcacgc cgaatcatcg
     4081 catcgacgta atgcactgaa agttcttgcc atgctttatc tcccacgtgc tgccagccgg
     4141 gtatttcctc caggaccatc aagccaagct cgtcacaggc gtctaaaaaa tgcggcgatt
     4201 gcgggtaatg tgaagtacga acaatattgc acttcaactc ttttcttaga atccaggcgt
     4261 cacgtcgttg cacgcgagca ggcatggcct ggcccacgaa tggataagtc tgatgacggt
     4321 ttagacctcg aagcttcaga tgtttgccgt tgagaaaaaa tccttcggga gtgaattttg
     4381 cttcgcgaaa accaattctg gtttcgtaag aatccacact ggtttcacct tccatcagcg
     4441 taactcgtac tagatagagc ttgggagtgg ctatatccca gagttgtact cccgcaaggt
     4501 tttgcagcgt tatgtcctga aagggatttg cagaagagat gttttgcgtt gctgaagata
     4561 taacctttgg tccatcgaac aactcaactt taagagcacg tgaggcccca ctcttactgt
     4621 tgataaagca acgcacttcg agtctgcgag tgtcctttag aacatctacc ggctttgcaa
     4681 aaacgttatc gatgaatgcg gaatttacaa aacgcaaagc gacatcccga tagataccgc
     4741 cgaacgtcag atagtcgatc tctcctccga agggaggaat gtcgttccgt tcggttgaat
     4801 caacttcaac tgcgagcagg ttgttgccgc gccagttcaa atgttcggtt acttcaaatg
     4861 agaagggggt ataaccgccg cgatattcgc cgaggaattg atcgttcaag gtcact
//
LOCUS       DASSFP010000015         9232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_151378, whole genome shotgun sequence.
ACCESSION   DASSFP010000015 DASSFP010000000
VERSION     DASSFP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151378"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFH91_00320"
     CDS             complement(<1..965)
                     /locus_tag="VFH91_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861446.1"
                     /translation="MKSLSHSSLTTYAILPTLVILITAVFAAGQSTGSAEDTSSSTPV
                     LRRIDRARALAAAHQLQPAATELENIRASVNDVTLRNVATLMLLGIYLENGTYGRAQS
                     LLEEAFQTRAAQKDESIRTYFAAAGQTVNGIRSRLARYRSFGLNPTDVDLPVEANTDL
                     DRMRSLTERVIAQAKEISQEQGRGYDALALQEDVLGIRLSLARDNSDRDRWQTEYVDA
                     RERLALFQRAASLGRSAALDALTSRIPNPFQAPKTDDAGAGADKGLPTTTVLPVSGTA
                     PSAGAPASSPSPTTSTDNGEPKLVSTGSLSGRETKRVTPSYPAIA"
     gene            complement(1156..2532)
                     /locus_tag="VFH91_00325"
     CDS             complement(1156..2532)
                     /locus_tag="VFH91_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6861447.1"
                     /translation="MLVSSVPTNASQTRRQVDLLILGGTVVTMDATRRVLEDGAVAIT
                     AGRIVAVGPRAEIERAYQSQQTVDAKGQLITPGLINGHTHIPMVLFRGLADDLDLQDW
                     LTKYIFPAEAKNVSEEFVRVGTRLGLAEMIRGGTTTYCDMYYFEDAIADETSKAGVRG
                     VLGETVIDFPVADNKNNAEAMAYVEKFVKRWQGNDLIVPAIAPHAPYTVSEEHLKAAR
                     AFSDRTGALIVTHVAETKKELEDSLKSKNASPVQYLERIGFLNDKVIAAHVVWPQGDD
                     LETLKRRGVGVVHNPQSNMKLASGVAPVPKMLSDGLFVGLGTDGAASNNDLNMWEEMD
                     TVAKLHKVFSGDPKIISALQAFELATIRGAQALHLEKEIGSLETGKRADLLLIERDSL
                     NQTPMYNLYSDLVYATKANDVQTVIINGRIVMRDRKLLTLDETSIKKDAIVFRERIIK
                     SLSPPTGQ"
     gene            complement(2587..3003)
                     /locus_tag="VFH91_00330"
     CDS             complement(2587..3003)
                     /locus_tag="VFH91_00330"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET6861448.1"
                     /translation="MSEKNSESLIEIAKQARLQSVAPFSNFQVGAAIRTEGGKVYTGC
                     NVESASYGLTVCAERVAIWKALSEGERNFTELAVVADTESLTPPCGTCRQIIWEFARG
                     AKIVFANLDGKSETFEIAELLPRAFDARFLKSGHVR"
     gene            complement(3000..3635)
                     /gene="udk"
                     /locus_tag="VFH91_00335"
     CDS             complement(3000..3635)
                     /gene="udk"
                     /locus_tag="VFH91_00335"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET6861449.1"
                     /translation="MIIGICGGTGSGKTTVANRILESVSAEQVVFIQQDSYYRNLKDL
                     PLDYRQLANFDHPDALDNDLLVNHVRKLKAGEPIDLPLYDFRTNNRVSEVRPVEPRPI
                     VIVEGILIFAEPRLLEQMDIKVFVDTPDDIRFIRRLRRDVAERGRTVESVIEQYLATV
                     RPMHMQFVEPSKRHADVIIPEGGHNLVSIDLLSGKIRERLASALSTIGGRA"
     gene            complement(3660..4511)
                     /locus_tag="VFH91_00340"
     CDS             complement(3660..4511)
                     /locus_tag="VFH91_00340"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET6861450.1"
                     /translation="MAERVASASLYERAEHAARLIRSRISVEPRIAIVLGSGLGGFAD
                     DFEDAVAVPYSEIPGFVRSTAQGHAGRLVVGTIDKVPVVAMQGRVHYYEGYSLEEVTF
                     PVRTFKLLGIKTLILTNASGGINVQLTQGALMVLSDHLNLMGDNPLRGPNDDRFGPRF
                     PDMSAVYSPALQEIVVEEARDLGVEIRRGIYAALGGPSYETPAEIHLLRNLGADAVGM
                     STVPEAIVARQMDMEVLGISCITNMAAGIGDEPINHDEVMATGDRVRSTFTQLLRSVL
                     GRVHARV"
     gene            complement(4519..5442)
                     /locus_tag="VFH91_00345"
     CDS             complement(4519..5442)
                     /locus_tag="VFH91_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6861451.1"
                     /translation="MKDIFTLELLWSTIRLAAPLILAALGGLFSERSGVINIALEGKM
                     LAGAFTAAAVTYAADVKLGLGSGSPWLGLISAIAAGVFLAIIYGIVCIRFKADQVVSG
                     AALNILMLGIPGFLSGALFLSSGSTPQLPKDHLLPQDPRIIGLALALIVAASWYVLYR
                     TPFGLRLRSVGEKPEAADAAGISVEKLRYSGVLLAGALAGMGGAYLSIGQSSLFTRNM
                     SAGRGFIALAALIFGKWRPVQTLLACLLFGFTEAFSNQIQGLIKLPSGEDIPVQFIAI
                     VPYVLTIIVLAGFIGSSRPPLALGIPYRKEK"
     gene            complement(5448..6518)
                     /locus_tag="VFH91_00350"
     CDS             complement(5448..6518)
                     /locus_tag="VFH91_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6861452.1"
                     /translation="MTKLLRELIFPLIAVVAAFIVGGILILLVGDNPITAYRLLLGSA
                     FSWPDGIGYTLFHATPLIFTGLAVLVGFRCGLFNIGAEGQLYIAAFATAWVGITFANL
                     SAWLLVPMCCIAAIGAGAAWAAIPGVLKARFGSHEVINTIMLNFIAVALVSYFTQYHY
                     RAPGDPILETTAIGYGAHIARLGKFIPSFPERIPLNLAFLLALLCCLLVYLFLWRTKW
                     GYEIRATGSNPSAAEYGGISVKKQIVIAMAVSGALAGMVGINEVLGYRYRYYDGFSDN
                     YGFTGIAVALLGRNHPVGVVLSALLFAVLQHGGIYVDGFSEHVTKDIVQVLQGIIILF
                     VAAEGLFRGPFSRFGLLKQSKV"
     gene            complement(6633..8141)
                     /locus_tag="VFH91_00355"
     CDS             complement(6633..8141)
                     /locus_tag="VFH91_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6861453.1"
                     /translation="MLQLRDITKTFGDVVANDRVNIVIKPGTIHAIVGENGAGKSTAM
                     RIAYGFYTPDSGEIIVDGQPRDIRSPHDAIALGIGMVHQHFMLVEPMTVAENIVLGAE
                     PGSAFAMDLKSAATQIRLLSEEFKLAVNPNAIVEHLSVGQQQRVELLKALYRHARLLI
                     LDEPTAVLTPLEVEEFFVILRRMRDQGKTVIIITHKLSEVLAISDEVTVMRDGKVVGE
                     VQTRDTNAADLARMMVGREVLLRVEKPEAKPGAPELVVEKLSVTTADGTKRVNDVSFN
                     VRRGEIVGIAGVEGNGQTELIEALAGLTSGRLSGQMLFEGKDIGLLGARSRRELGIAH
                     IPEDRHRRGLILDFDLAENSILGVHYRPPIAASVGGVFLDGHAIDQRCKEIIEAFDVR
                     PPNALLAARSLSGGNQQKLIIGREFRVDPKLLLVSQPTRGVDIGAIEFIHRKLVELRD
                     AGCAILLVSAELEEVTSLSDRLLIIHEGRIVGEVDPKVASVEEIGLLMTRGH"
     gene            complement(8214..8594)
                     /locus_tag="VFH91_00360"
     CDS             complement(8214..8594)
                     /locus_tag="VFH91_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6861454.1"
                     /translation="MEKTNFENLHIYRLSEKLADQIWRIVIGWEFFAKNTVGIQIVKA
                     ADSVGANIAEGSGRGTEPELRRFLRVARGSLYETQHWLRRAYKRNLLTQNQIDELVPV
                     IRELTPKLNSYLGSIGSLKKRFRS"
     gene            complement(8669..>9232)
                     /locus_tag="VFH91_00365"
     CDS             complement(8669..>9232)
                     /locus_tag="VFH91_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6861455.1"
                     /translation="IGFLGGMDIGLIHRFEKGYEEGAKSVNPNIRIIQNYVGVTDSAW
                     NNPGKGKELSLAQISKGADVIFTAAGNSGLGAFDAVEQQGKQNGRATHFVIGVDSNQN
                     MVKPGFVLTSMVKRVDNAVYNIVQEVVNGNFSPGLHVFGLDKDGVGYAMDENNKDIVS
                     AEAIQEAEAAKQKIISGQIKVTDAMGQ"
BASE COUNT         2289 a         2491 c         2187 g         2265 t
ORIGIN      
        1 ttggctatag cgggatagct gggtgtcact cttttggttt cgcgaccact caaagaaccg
       61 gtgctaacta acttgggctc tccattgtcc gtgctggttg tgggagaagg gctgctggcc
      121 ggagctccag cagagggtgc cgtacctgag actggcaaaa cggtagttgt tgggagtcct
      181 ttgtcggcac cggcgccggc gtcatctgtt ttgggagcct ggaagggatt tgggattctt
      241 gaggtcaacg catcgagggc ggccgatcta ccaagagaag cagcccgttg gaacaaggcc
      301 agcctctctc ttgcgtcaac gtattctgtc tgccaacgat cgcgatcaga attgtctctt
      361 gccagtgaaa gtcttattcc gagtacgtct tcctgcaatg ccaacgcatc ataaccgcgt
      421 ccctgttcct gcgatatctc cttcgcttga gcaataactc gctcagttag gctccgcatg
      481 cgatccagat cagtgttggc ttctactggc aaatcaacat cagttggatt caacccaaag
      541 ctgcgatacc gtgcgagtcg tgagcggatt ccgtttaccg tttggcccgc agcggcgaaa
      601 tatgtccgaa tcgactcatc tttttgggca gctcgagtct gaaacgcctc ttccagcagg
      661 gattgagcgc ggccgtaggt accattttcg agataaatcc caagcaacat tagcgttgcc
      721 acatttcgca acgtgacgtc gttaaccgaa gcacggatgt tctcgagttc agtggcggct
      781 ggctggagtt gatgagcggc agcaagagct cgtgctcggt caattcggcg aagaactggc
      841 gttgatgatg aagtgtcttc cgctgagcca gtggactgcc cagctgcgaa aactgcagtg
      901 attagaatta cgagtgtcgg gaggatcgca taagttgtta aagaagaatg agataaagat
      961 ttcaaatggc ttactccaga aataagaaaa gcgagcatga ggctcgcttt agtaacttga
     1021 cgacctatta tggccgctca actcaaatga ggcaagcacc tttggccttt ggcctttggg
     1081 cttggtcgtt ggcctctcgg ctagggaccg aaggtcaaag ttcaaaggcc aaaggtctaa
     1141 ggccaaaggc cgccgctatt gtcctgtcgg tgggcttaga ctcttgataa ttcgttcccg
     1201 aaatacgatg gcatcctttt tgatactcgt ctcgtcaagc gtcaacaact tacgatcgcg
     1261 cataacaatc ctgccattaa taatcaccgt ctgaacatcg ttagccttcg tggcatagac
     1321 taagtccgaa tacaggttat acatcggagt ttgattgagg gaatcacgct caatgagcaa
     1381 taagtctgcg cgcttaccag tttctaaaga accaatctcc ttttcaagat gaagggcctg
     1441 cgctcctcga atggtggcca actcgaacgc ttgcagggca gaaattattt ttgggtcacc
     1501 actgaacact ttatgcaact ttgcaacggt gtccatctct tcccacatgt tcagatcatt
     1561 attcgacgct gcaccatcag tcccgagccc gacaaacaaa ccatcgctca acattttggg
     1621 aacgggcgcg acacctgagg ctaacttcat attggactgc ggattgtgga caacaccaac
     1681 tccacgccgc ttgagcgttt ccaggtcatc gccctgcggc cacaccacgt gagccgcgat
     1741 caccttatca ttcagaaatc ctattctttc gagatattgg accggactgg cattcttcga
     1801 cttaagactg tcttcaagct ccttcttagt ttcagccaca tgagtaacga ttagtgcacc
     1861 agttcgatcg gaaaaggctc gcgcggcctt aagatgctcc tccgaaactg tgtaaggcgc
     1921 gtggggagcg atggcgggaa caatcaaatc gttcccttgc cagcgcttta cgaacttctc
     1981 gacgtaagcc atcgcttcag cattattctt gttatcagca actggaaagt cgatgacggt
     2041 ttcgcccagt actcctcgaa cccccgcctt gctggtttcg tcggcaattg catcctcgaa
     2101 gtagtacata tcgcagtaag tagtggtccc accgcgaatc atttctgcta aacctaagcg
     2161 tgtgccgacg cgaacaaact cctcactcac attcttagct tcagcgggaa agatgtactt
     2221 tgtgagccag tcctggagat cgaggtcatc tgccagccca cgaaacaaca ccattggaat
     2281 atgggtgtga ccatttatca atcccggggt gatcagctga cctttggcat caactgtttg
     2341 ttgcgactga taggctcgtt cgatctcggc gcgtggtcca acggcaacta tgcgaccggc
     2401 tgttattgcg acggctccat cttctaacac acgccgtgtt gcatccattg tcacgacggt
     2461 tccgcctaag ataagtagat ctacctgccg cctggtttgt gaagcgttgg ttggaacgga
     2521 acttacaagc atggttgcaa gaatcactaa atgaagaatt cggtcaagca taggttttcg
     2581 aatgaattat cgaacatgtc cgctcttaag aaatcgggca tcgaaggctc gcggcaacaa
     2641 ctcagctatc tcaaacgtct cgctcttgcc atcgagattt gcaaaaacaa ttttcgcgcc
     2701 gcgcgcaaat tcccaaatta tttgtctaca agttccgcaa ggtggcgtca gcgattctgt
     2761 atcagccaca acggcgagtt cggtgaagtt gcgctcccct tctgacaagg ccttccaaat
     2821 ggcaacgcgc tcggcgcaaa cggtaagtcc gtagcttgca ctttcgacgt tgcaaccggt
     2881 gtagactttg ccaccttcgg ttctgattgc ggctccgact tggaaattgg aaaatggcgc
     2941 cactgactgt agacgcgcct gtttggcaat ctcaattaga gactctgaat tcttttcgct
     3001 caagctcgac ctccgatagt tgataaagca ctggccagcc tttcgcgaat ttttccgcta
     3061 agcaaatcaa tgctcacaag attatgtcca ccttccggaa tgatgacgtc tgcgtgacgc
     3121 ttagatggct ccacaaactg catatgcatc ggcctgaccg ttgccaaata ctgctcaatg
     3181 actgattcaa ccgtccggcc acgttcagcg acgtcacggc gtagtcttct gataaaccga
     3241 atatcatccg gagtatcgac gaacaccttg atatccattt gttccagcaa tcgtggttct
     3301 gcaaatatca gaatgccttc gactataaca attggccttg gttcaaccgg tctgacttca
     3361 ctcacacgat tgttagttcg gaagtcataa agcggcaagt caatcggttc gcctgccttt
     3421 aacttgcgca catgattcac gagcaagtcg ttatcaagcg cgtccggatg atcaaagttt
     3481 gcgagctggc gataatcaag gggcaggtct tttagattgc ggtagtagga gtcttgctgg
     3541 atgaaaacaa cctgttcagc gctgactgat tcaagaatcc tatttgcaac tgtcgttttt
     3601 cccgaaccgg tgcctccaca aatgccaatt atcatgcgaa acttccaatc ttcccctctt
     3661 caaactctcg cgtgcacacg gcccagaacg ctgcgcaaca gctgcgtgaa cgtcgatctc
     3721 acgcgatctc cggtcgccat cacctcgtcg tgattgatcg gttcatcacc tataccagca
     3781 gccatgttcg tgatacagga tatgcctaag acttccatat ccatctggcg agcaacaatt
     3841 gcttccggga ccgtgctcat gccgaccgca tcagctccga ggttcctcag gagatggatt
     3901 tcagcgggtg tttcataact cggtccaccc agagcggcat agataccacg tctgatctca
     3961 acgcccagat cgcgcgcttc ttcaacaaca atctcctgca acgccggcga ataaacggcc
     4021 gacatgtcgg ggaaacgtgg tccaaaacga tcgtcatttg gtccacgtaa aggattgtct
     4081 cccatcaaat ttagatggtc actcaaaacc atcaaggcgc cctgagtaag ttgaacgtta
     4141 atgcctcccg aggcgttggt aagaatcaat gttttgatgc ccagtaactt aaaggtcctg
     4201 accgggaacg tgacttcttc gagagaataa ccttcgtagt aatgtactct cccttgcatc
     4261 gcgacgaccg gtaccttgtc tatggtgccg acgaccaggc gccccgcgtg gccctgggcc
     4321 gttgatctga cgaaaccagg tatttcactg taagggaccg cgactgcatc ttcgaaatcg
     4381 tcagcaaagc cgcctaaccc gctgccaagc acaatcgcta ttcgcggttc aacgctgatt
     4441 cgtgatcgaa ttaatcgggc tgcatgctct gcgcgctcgt acaaactggc gcttgcgact
     4501 ctttcagcca tcacttcgct atttttcctt cctgtacggt atgccaagag ccaggggcgg
     4561 tcgcgacgat cctataaagc cagccaaaac aattatggtc aagacgtagg gaacgattgc
     4621 gataaactga accgggatat cttctccaga gggcaacttt atcaggcctt gtatttgatt
     4681 tgaaaatgcc tcggtgaacc caaagagtag acacgccagg agagtttgca ccggacgcca
     4741 cttgccaaat atcaacgctg cgagcgctat aaaccctcgg ccggcactca tgtttctcgt
     4801 gaacaatgaa gattgcccga ttgaaagata ggcgccaccc ataccggcga gtgctcctgc
     4861 aagtaacact ccggagtaac gcaatttttc gacactgata cccgccgcgt ctgcagcttc
     4921 gggcttttct ccaaccgacc gtagtcttaa accaaatggc gtccgataga gaacatacca
     4981 gctggcggcg acgatcagcg ccagggccag gccaatgatt cttggatcct ggggcaacag
     5041 atgatctttc ggcaactgag gggttgaacc tgaagatagg aataacgcac cgctaagaaa
     5101 tccggggatg cctaacatca gaatgttcaa ggctgctcca ctgacaacct ggtctgcttt
     5161 gaaacggata catacgattc cataaatgat cgccagaaag acaccggcgg cgatcgctga
     5221 gattagaccg agccagggac tgcccgatcc taatccaagt tttacatcgg cagcatacgt
     5281 gacagcagct gcggtgaaag cgccggcgag catctttcct tcgagcgcta tgttgataac
     5341 tccggaccgc tcagagaaca acccacccaa cgcagcaagt atcagcggcg ctgcaagtct
     5401 gatcgtggac cacaataatt ccagagtgaa gatgtctttc atcgttttta aactttcgac
     5461 tgtttcagta aaccaaatct gctgaatgga ccacgaaaca ggccttcggc agctacgaag
     5521 agtatgatga tgccctggag cacctgcaca atatccttgg ttacgtgctc agaaaaaccg
     5581 tcgacataga tgccgccatg ttggagcact gcaaatagta gcgccgaaag gacaactcca
     5641 acgggatgat tacgcccaag aagcgcgacg gcgattccgg tgaacccgta gttgtcggag
     5701 aacccatcgt agtagcgata acgatagcca agcacttcat tgatccccac catacctgcc
     5761 aacgcaccag agactgccat cgcaatgaca atctgctttt taacagaaat gccaccgtac
     5821 tcggcagccg aaggattact tcctgtggcg cgaatctcgt aaccccattt agttcgccat
     5881 agaaaaagat aaacaagcaa gcaacagagg agcgccaaaa ggaatgccag attcagtgga
     5941 atgcgttcag gaaaactcgg aataaatttc cccaggcgcg caatgtgtgc gccgtagcca
     6001 atcgctgtag tttccaggat cggatctccc ggagcacgat aatgatattg agtgaaatag
     6061 ctaactagcg cgaccgctat gaagttcaac atgatggtgt tgataacttc gtgtgatcca
     6121 aatcttgctt tcagtacccc cgggatcgcc gcccatgcgg caccagcgcc gatcgcggcg
     6181 atgcaacaca ttggaacaag tagccacgcc gacaggttcg cgaatgtaat accaacccat
     6241 gctgttgcaa acgccgcaat atagagttgt ccctctgcac caatattgaa tagaccacag
     6301 cgaaaaccaa ccaggaccgc cagtccggta aagattaaag gggtcgcgtg aaataacgta
     6361 tagccaattc cgtcgggcca cgagaacgcg ctgccgagca acaatcggta ggcggtgatg
     6421 ggattatctc ccaccaataa aataagaatg ccgccaacga taaacgcggc gacaacagca
     6481 ataagcggga agatcagttc gcgcagcaat ttggtcattt aggattcttg tgctctgttt
     6541 tgatctttga tctttggtct ttggtcttgt gcttacacct attcgaaact agcgacgccc
     6601 ccgctctctg gacccattag catcgagctc gttcaatgcc ctcgcgtcat caacaaacca
     6661 atctcttcta cggacgcaac ctttggatcg acttcaccta caattcgacc ctcgtgaatg
     6721 atcaacaatc gatcagaaag acttgtaact tcttcgagtt ctgctgaaac caacaaaatc
     6781 gcacatcccg catctctcag ctccaccagc tttcggtgaa tgaactcgat cgcgcctata
     6841 tcgactccgc gtgttggctg cgagactaaa agtagcttcg gatcaactct gaactctctg
     6901 ccaattatca acttctgttg atttccccct gataaagaac gagctgccag caaagcgtta
     6961 ggaggacgca cgtcaaaggc ttcgataatc tctttgcaac gctggtctat agcatggcca
     7021 tcaagaaaca cgccaccgac tgacgctgca attggcggcc tgtaatgaac acccagaata
     7081 gaattttcgg caaggtcaaa atcgagtata agtcctcgtc ggtgccgatc ctcggggata
     7141 tgagcgatgc ccaactctcg tcgcgaacga gcacccagca agccaatgtc ttttccttca
     7201 aaaagcatct gccccgaaag gcgaccggac gtcagtccag ccagcgcttc tatcaactca
     7261 gtctgaccat tgccttcgac gccggcaatc cccacgattt cgccacggcg cacattgaat
     7321 gagacgtcat taactcgttt tgttccgtcg gcggttgtga ccgatagttt ttcgaccacc
     7381 agttccggag ctcccggctt agcttcaggc ttttcgacgc gcaggagaac ctctcggccc
     7441 accatcattc gtgcgaggtc ggccgcgttt gtatccctgg tctgtacctc accgactact
     7501 ttgccatcac gcatcacagt tacttcatcc gaaatcgcca gcacttcact tagtttgtga
     7561 gtaatgatga tgaccgtctt gccctggtcg cgcatgcgac gaagaatgac aaaaaattct
     7621 tcaacttcaa ggggcgtgag cacggctgtc ggctcgtcga gtatcaaaag tcttgcgtgt
     7681 cggtagagag ctttaagcaa ctccactcgt tgttgttggc ccaccgaaag gtgttcaacg
     7741 atcgcatttg gattaacagc aagcttgaat tcttcggata gtagtctgat ttgggtcgcg
     7801 gcgctcttaa ggtccattgc gaaggcagaa ccaggctctg cgccgaggac tatattctcg
     7861 gcaacagtca tcggctcaac gagcatgaag tgttgatgga ccataccgat ccccagcgcg
     7921 atcgcgtcgt gaggcgatcg aatgtctctt ggttgaccat cgacaatgat ctctccgcta
     7981 tcgggagtgt agaaaccata agcgattcgc atcgccgtgg acttaccggc gccattttcc
     8041 ccgacgatcg cgtgaatggt gcctggctta atgacaatgt ttaccctgtc attcgccacc
     8101 acatcaccaa aggtttttgt tatgtcacgg agttgaagca tagattgttg gtctttggcc
     8161 ttcggtcttt ggccttggtc gactttcaat aatcatatcg acgctagccg aaactagctt
     8221 cggaagcgct tctttaatga gccgatagaa cctaagtacg agttcagttt tggagtcaac
     8281 tctcgaatta cgggcaccaa ctcatctatt tggttctgcg tcaaaagatt tctcttgtat
     8341 gctcggcgta accaatgctg agtttcatat aacgaaccac gggccaccct tagaaaacgt
     8401 cttaactccg gttcagtgcc gcgaccgctg ccttcagcaa tgtttgctcc cacgctgtcc
     8461 gccgccttaa caatttggat gcctactgtg ttcttcgcga agaattccca gccgatcacg
     8521 atccgccaga tctgatcggc aagtttctct gacagcctgt aaatgtgaag attttcaaaa
     8581 ttagtctttt ccatctcggt ttctcctgag gaaagattga taagaccaca aaattcaaag
     8641 accaaaggcc aaagatcaaa ggccagcact actggcccat tgcgtcggtg acttttatct
     8701 ggccggagat gattttttgc ttggcggcct ctgcttcctg gatggcttct gctgaaacaa
     8761 tgtctttgtt gttctcgtcc atcgcataac cgacgccgtc tttatccaga ccaaaaacat
     8821 gtagaccggg agaaaagtta ccattcacaa cttcttgcac aatgttgtag acggcgttgt
     8881 ctaccctttt caccatgctg gtgagaacaa atccaggctt taccatgttc tggtttgagt
     8941 ccacgccaat cacaaagtga gtggctcgtc cattctgttt accttgttgc tcaaccgcat
     9001 cgaacgcacc aagcccagaa ttcccggcgg cggtgaagat aacgtccgct cctttactga
     9061 tctgggccag cgagagctcc ttgcctttgc caggattatt ccacgcagaa tcggttactc
     9121 cgacgtagtt ttgaataatg cgaatatttg gatttaccga tttcgctccc tcttcatatc
     9181 ctttttcaaa gcgatgaatc agaccaatgt ccatccctcc caggaatcct at
//
LOCUS       DASSFP010000016         4401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1193940, whole genome shotgun sequence.
ACCESSION   DASSFP010000016 DASSFP010000000
VERSION     DASSFP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193940"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..808
                     /locus_tag="VFH91_00370"
     CDS             221..808
                     /locus_tag="VFH91_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6861456.1"
                     /translation="MARKWSNLNLPGALHYVTGNCINRLPVFTEPACCKAFLEQLEKL
                     NSEWPAKLIVYVLMPDHFHFISNPCDGRIIEFCLELKSKAAKAIVQTSRRFQFPINED
                     GHQVWQQSFKAMPLWSEWMIKQKINYIHANPVKAKLVKSARDYYWSSFRSFYCQGDEP
                     LKVDHDWWWPDDAEKLSKAMKKMGWHSWWKRDEDE"
     gene            complement(990..1214)
                     /locus_tag="VFH91_00375"
     CDS             complement(990..1214)
                     /locus_tag="VFH91_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET6861457.1"
                     /translation="MNIKDFIEVDPQKMSGTPCFTGTRVPLTHLFDYLEAGDSLGVFL
                     SDFPSVTREQALGVLELMKEKLLTEYETAA"
     gene            1310..4054
                     /locus_tag="VFH91_00380"
     CDS             1310..4054
                     /locus_tag="VFH91_00380"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET6861458.1"
                     /translation="MGSLWDVDDQSSRLTELTSHDSDVKEAPLRRDVRSLGILLGEVL
                     KEQVGEQLFAAVEELRLLLIEQRDSEAVDGGNPLIKRAEEIVSRLTIDQSHQMTKAFA
                     IYFELTNLAETNHRKRRRRAAQSSTEPIAQPGSFLGTLRRMRDAGVSAQQARDWLDQI
                     EVTLVFTAHPTEVARRTVLFKRQRIALELEQLDHLPLPERDAEKHQQAILTEITSLWQ
                     TDEVRRRRPTVKDEIRMGLDYYPTVLFDTLPIFYEGLADDFREAYGVELRARDLPRLV
                     RFGSWIGGDRDGNPSVTPECTRDALEIAREMILDYYLERIEDLIWRLTPSTLQVEVSG
                     PLLAGLEYYAANISAPGLNPERHGPNEIYRRYLDYVLVRLKASKSAESHAYATSQEFK
                     NDLVLIRDSLAANRGEKIARLLVDPLLRQVETFGFHLHTLDVREHARVHSQALAELSS
                     AHTESASFQVRQSVSDETKKLLDSLRMVRNLKQTFPTEAIRSYVISGAKSATDISSLI
                     WLAQLSGVRVEGSVDETGLMPVPLFESIEDLRNCAEICRTVWTNPDYNQLLDSWGRRQ
                     EVMLGYSDSNKDGGMLTSTWEIFKAHRSLHDIAKECNVKLRLFHGRGGTVGRGGGPTH
                     HAIIAQPAGAFDGHIKITEQGEVMNWKYSDPVLAERNLELVIAASLEALSRPDGAFEL
                     DQESESAIETMSRDAFEYYREKIAENDDVLSYFSEATPVNELEFARIGSRPAKRSNRR
                     GLDDLRAIPWVFGWMQSRNVLPAWFGVGFALDRFAKSGAGKRQLLQNLIDRFPLFHDL
                     IDNVELGMAKADLSIARHYAGLVADQSMRERVLQLIFEEFERTKRVVLELTGQSTLLE
                     KNPVLARSIKLRNPYVDPLSLIQVELLRRKRAGEDGDDLNYALAATINGIAAGLRNTG
                     "
BASE COUNT         1105 a         1108 c         1131 g         1057 t
ORIGIN      
        1 aggtagagtt gctgatgttt gccgctctgc ccccttccca actgcatgat tctgggctgt
       61 ttctcagttg gctggcggac atcaatacgc tacctactcg aacctagcgc cgcccccgct
      121 cttcacacca tactcaatta aaacctatca aagtcttgaa ctcctcaaaa tcctaagcta
      181 acatcctcaa accaagtcct cagaaaatgg agctcctcac atggctcgca aatggtccaa
      241 cctaaatctt cccggtgcac tgcattatgt aaccggcaac tgcattaacc gactcccagt
      301 attcactgaa ccagcatgct gcaaggcatt ccttgaacag ctggagaagc tcaatagtga
      361 atggccagca aaactaatag tgtatgtctt gatgccggat cactttcatt ttatctccaa
      421 tccctgcgat ggtcgcatca tcgaattctg tctagaactc aaaagcaaag cagccaaagc
      481 tattgtgcaa acctctcgtc gatttcaatt tccgatcaat gaagatggtc atcaggtttg
      541 gcagcagagc tttaaggcca tgccgctgtg gagtgagtgg atgatcaagc aaaagatcaa
      601 ctacattcac gccaatcctg tgaaggcgaa actggtaaag tcagctcgcg attactactg
      661 gtctagcttt cgttctttct attgtcaggg agatgagccg ctaaaagttg atcatgattg
      721 gtggtggcca gatgatgctg aaaagttgtc aaaggcaatg aaaaagatgg gatggcacag
      781 ttggtggaag agagatgagg atgaatgaag ttattgaact gtctaggagt gaagagcggg
      841 ggcgacgctg ggttcgagtt ggtagcgttg ttgatgtccg ccgctctgcc cccttcccaa
      901 ctgcatgatt ctggttagtt gctcaatggg ccgctctgca cgttccaaac tgcatgattc
      961 tggctgtcag tcgttgagga acacattcgt caagcagcag tctcatactc tgtgaggagt
     1021 ttttctttca tgagttctag aacaccaaga gcctgttctc gagtcacaga aggaaagtcg
     1081 ctgagaaata cgcctaaact atctccagct tctaaataat cgaacagatg agttaacggg
     1141 acgcgcgttc cggtgaagca cggcgtgccg ctcatctttt gaggatccac ttcgataaaa
     1201 tccttaatat tcatgacact ctctggcgca acattgtagc ctgtacaact acagattgca
     1261 cagatcacgt aaactgataa tctgtcaaaa tctagttgca aggagaaaaa tgggttccct
     1321 ttgggatgtt gatgatcaat ccagtcgcct gactgagctg acgagccacg acagcgacgt
     1381 caaggaagct cccttacgtc gtgatgtgcg ctcgctcggc atcttgcttg gcgaagtact
     1441 taaagagcag gtcggcgagc aactgtttgc tgcagtcgag gaactgcggc tgctgttgat
     1501 tgaacaacgc gactcagaag cagtcgacgg cggcaatcct ttaattaaac gcgccgagga
     1561 aattgtgagt cggctgacga tcgatcaatc gcatcagatg accaaagcgt ttgcgatcta
     1621 ttttgaacta accaatctcg ccgagaccaa tcatcgcaaa cgacgtcgcc gagctgctca
     1681 gagttctact gaaccaatcg ctcaaccagg ctcatttctg ggcacccttc gacgtatgcg
     1741 tgacgctggt gtcagtgcac aacaggcacg cgactggctc gatcagatcg aagtaaccct
     1801 ggtcttcacg gcgcatccca ccgaagtcgc ccgccgcact gtccttttta agcgacaacg
     1861 gattgccctt gaacttgaac aattggatca cttgcccctg ccagagcgcg atgccgaaaa
     1921 acatcagcag gcgatcctca cagagataac ttctctctgg cagactgacg aggtccgtcg
     1981 tcgacgacca actgtaaagg acgagattcg aatgggtctc gactactacc cgactgtgct
     2041 cttcgatacc ttgccaatct tttacgaagg attggcggat gattttcgtg aggcttacgg
     2101 tgttgagtta agagcgcggg acttgcctcg tttagtccga tttggttcat ggatcggtgg
     2161 cgatcgcgac ggcaacccat cggttacgcc tgaatgtacc cgcgacgcac ttgagatagc
     2221 tcgcgagatg attctcgact attacctcga gagaattgag gatctgatct ggcggctcac
     2281 tccatcaact cttcaggttg aagtctctgg accattgctc gcagggttag agtattacgc
     2341 cgcaaatatt tctgcgcccg gtctaaatcc ggagagacat ggtccaaatg agatttaccg
     2401 ccggtatctg gactacgttc tcgtaagact caaggcctcg aaaagtgcgg agagccacgc
     2461 ctatgcgacg tcacaagagt tcaaaaatga tttggtgttg atacgcgaca gcctggctgc
     2521 taaccgcggt gagaagattg cacgactcct ggtcgatccc ttgttaaggc aagttgagac
     2581 ttttggcttt cacctgcaca cgctcgacgt tcgagaacac gctcgcgtac acagtcaagc
     2641 cttagcagag ctttcgagtg cgcataccga atcagccagt tttcaagtgc ggcagtcggt
     2701 atcagacgag acaaagaagc tcctcgattc cctgcgcatg gttagaaatc tgaagcaaac
     2761 atttcccacg gaagcaattc gtagctacgt gatcagcggc gcaaaatcag caactgatat
     2821 ctcctcgctc atctggctgg cccaattatc aggagtacga gtcgaaggtt ctgtggatga
     2881 aactggattg atgccggttc cgctgttcga gtcaatcgaa gaccttcgca attgtgctga
     2941 gatttgtcgc accgtctgga ccaaccccga ctacaaccag ctactcgatt cgtggggccg
     3001 gcgacaggaa gtaatgctcg gctattcaga ctccaataaa gacggcggga tgctcaccag
     3061 tacgtgggaa atcttcaagg cacatcgttc gcttcacgac atcgccaaag agtgcaacgt
     3121 gaagttgagg ttattccatg gccgaggcgg aacggttggc cgcggtggtg gtccaactca
     3181 ccatgcgatc atcgctcaac ctgccggcgc atttgatggc catatcaaga ttacggaaca
     3241 aggcgaggtg atgaactgga agtattctga tcccgttctg gccgaacgca atctcgaact
     3301 ggttatcgca gcttcattgg aggcgctgtc acgacccgac ggcgctttcg agctcgatca
     3361 agagagtgaa tcagcgattg aaactatgtc gcgtgatgcg ttcgaatact atcgcgagaa
     3421 gattgccgag aacgatgatg tgctcagtta tttctcagag gcgactccgg ttaacgaact
     3481 ggaatttgcg cgcattggtt cgcgacctgc gaaacgcagc aaccggagag gtctcgatga
     3541 cctgagagca attccctggg tgtttggctg gatgcaaagt cgcaacgttt tacctgcatg
     3601 gtttggtgtt ggatttgcac tcgacagatt tgcgaagtct ggcgcgggaa aacgtcaact
     3661 cctgcagaac ctgattgatc gctttccgct cttccacgat ctgattgata acgtcgagct
     3721 gggtatggca aaggctgatt tgtcgatagc tcgccactat gcaggcctgg tggcagatca
     3781 atcgatgcgt gaacgagtgc tccagcttat tttcgaagag tttgagagga ccaaacgagt
     3841 ggttcttgaa ctaaccggcc agtcgacctt gttggaaaag aacccggtgc tggcgcgttc
     3901 aatcaaactc agaaatcctt atgtcgaccc gttgagtctg attcaggttg agttgctgcg
     3961 acgaaaacgg gctggtgaag acggggatga tttgaactat gcgctggcgg caacgatcaa
     4021 tggtattgcc gcaggcctga ggaatacggg gtgagggtca atcaccgctt cctcagattg
     4081 cactgatgcc cgcgaagtgt gagctacttc agtgagtgat ctttcagagt gtcggaacaa
     4141 cgattcgcgc aaatacgtgg cgcatttatt caaactgacc ccttttccat cccttttgcc
     4201 tccccggtgg ttcaaccagg acagatcagg agccagcgat cgaggtaaga gagacgcgtt
     4261 ctttccccaa acaggaaaaa cccctctttg acactaatga ctattttcag gaaaacgtta
     4321 caaagaaact ttggagtgcg gcggcccggc gccgctttga cacataatca agtacccgga
     4381 taacagagcg ccgccgggcc g
//
LOCUS       DASSFP010000017         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1938433, whole genome shotgun sequence.
ACCESSION   DASSFP010000017 DASSFP010000000
VERSION     DASSFP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938433"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFH91_00385"
     CDS             <1..1147
                     /locus_tag="VFH91_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET6861459.1"
                     /translation="YFDGPGGTQVPRYVVDQMSDYLFHHNANTHWQYPTSAETDAAIA
                     KAREVLAEFLNASADEIAFGANMTTLTFHLSRALSRNLNAQDEIVVTDLDHHANIDPW
                     RRLTKECGLVVKSVKFDINTGSLIQNELEKSIGSKTRIVAIGAAANALGTINDIKYAI
                     DLAHDAGALAFVDAVHFAPHALIDVQELDCDFLAMSAYKFYGPHIGVLFAKRRIFEEL
                     DFPKLVPAPDYAPENCETGTQNQEGMVGAGAAVEFLASLAGDSSMRNRLESVFNITHA
                     RNMKLFTTLWEALSKMPRVTLYGPTPDQPRTPTIAFTISGIESSEAARRLAEKGLFLS
                     HGDFYAATVVERFGLSDEGLLRAGCALYTTEEEINRLIEAVGELAHA"
     gene            complement(1286..2293)
                     /locus_tag="VFH91_00390"
     CDS             complement(1286..2293)
                     /locus_tag="VFH91_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861460.1"
                     /translation="MKEYRVCFLGFGNVGRALARLLQTKSSELRETYGIDWRIHGVAT
                     RRLGWLSSVKAFDATKIVSGDFGDATAQSSIQEWLNHVRPDVVFETTSLNPDSGQPAI
                     DYLKAALQYGAHAISANKAPIVYAYDELQQIANAQGKKYFFESAVLDSAPIFSLFREA
                     LPLARLRGFTGVFNSTTNVILETMETGRTFDEGVRMAQELGVTETDPSHDVEGWDSIV
                     KVCAIARVILKVKLLPSDVRREGIRGLDVATLQSAKAEGKPFKLVSRVKFADDDKVIA
                     SVRPEQVSVSDPLGSVRGTSLGVHFELDMIPGLTIISHRPNLQSTAYGLLADFVNAVR
                     A"
     gene            2359..3207
                     /locus_tag="VFH91_00395"
     CDS             2359..3207
                     /locus_tag="VFH91_00395"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002446444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET6861461.1"
                     /translation="MKQYHDLLQSILQHGSAHEDRTGVGTISHFGFQTRFDLSRGFPI
                     VTTKRVPFRWVAEELFWFLSGDTNEENLRARGVDIWAEWADDAHTAKFGREPGDLGPV
                     YGYLWRSFGGDYPERNGVDQIARLMDQLSSNPDSRRLIVTGWDPRQADKVDLPPCHTL
                     FQFKVENRQTLHCQLYQRSADAFLGVPFNISSYALLTHLVAHVADLEVGEFVYTLGDY
                     HIYKNHLEQVNELLSREPLPLPELKIEDPEKKLRGLEGLLSVKYEHLNLIGYKSHGKI
                     AAPVAV"
     gene            3220..3693
                     /locus_tag="VFH91_00400"
     CDS             3220..3693
                     /locus_tag="VFH91_00400"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET6861462.1"
                     /translation="MIIGIAAVDRQGAIGKDGKLPWHYSSDMKFFRETTSGHAVVMGR
                     RTWLTLNKPLKNRLNIVLSRDSSIEPAESLLVLRDLESVLSFNESLTTDLFVIGGAQI
                     YKAFLPHIERWFITEVPLTVKGADTFMPENYLKGFKRYEKQGLDEELIVSFYERS"
     gene            complement(3850..>4345)
                     /locus_tag="VFH91_00405"
     CDS             complement(3850..>4345)
                     /locus_tag="VFH91_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HET6861463.1"
                     /translation="AGSIVTACMEGTRPLLVEIQALVSSSKYGSGRRMTQGVDQNRVS
                     LMIAMLEKRAGLQLIGDDVFINIAGGLEIDEPAADLGVVAAIASSFKNSPVDPHTAVF
                     GEVGLTGEVRGAMQAGVRAREVQALGFKKIVMPASNASGLERLLGVRIVGVKSVEEAL
                     EELF"
BASE COUNT         1080 a         1169 c         1042 g         1054 t
ORIGIN      
        1 ctacttcgat ggaccaggcg gaacgcaggt tccgcgttat gtcgtcgacc agatgagcga
       61 ctacctgttt caccataacg caaacactca ctggcaatat ccaaccagcg ctgaaacgga
      121 cgccgctata gcaaaagcac gcgaagtgtt agcagagttt ctgaatgcgt cggccgacga
      181 gattgctttc ggcgccaata tgacaactct cacctttcat ctatctcgcg cattgagtcg
      241 gaacctgaac gcgcaggacg agattgtggt gaccgacttg gaccaccacg ccaacatcga
      301 tccctggcga aggctgacaa aagaatgcgg tctcgtggtg aagagcgtta agttcgatat
      361 aaacaccggc tcgctgatcc agaatgagct ggaaaaatct attggctcca aaacgagaat
      421 tgtcgccatc ggggccgctg cgaatgctct cggcacaatc aacgacatca aatatgcaat
      481 agaccttgcc cacgatgccg gagcacttgc atttgtcgat gcggtccatt tcgcaccgca
      541 tgcgttgatc gacgtgcaag agctcgattg cgacttcctt gcaatgtctg cttacaaatt
      601 ctacgggcct catatcggag tattgtttgc gaaacgcagg atcttcgagg aacttgattt
      661 tccgaagttg gtaccggccc cggattacgc tcctgaaaat tgtgagacgg gaacgcagaa
      721 ccaggagggt atggtcggcg ctggcgcagc agtagagttt ctcgcttccc tcgcaggcga
      781 cagttcaatg cgcaacagat tagagagtgt cttcaacatt acgcacgcga ggaacatgaa
      841 actctttacg acgctgtggg aggcgttgag taagatgccc cgggtgacac tttacggtcc
      901 tacacccgat caaccacgaa caccaacgat cgcttttaca atctctggta tcgagtcgtc
      961 cgaggccgcc agaagattgg ccgaaaaggg actctttctt tctcacggag atttttatgc
     1021 cgccacagtt gtagaaaggt ttggtctttc ggatgaggga ctgttgcgtg ccggctgcgc
     1081 tttgtacaca acagaggaag agattaatcg tctgatcgaa gccgtcggcg aactagctca
     1141 tgcatgatcg tcaaattcca ggatcctaat aggacctata ggattttata ggacctatag
     1201 gacttatagg acttatcccg atctacatga ctacccaatc gaccacacaa acacagccct
     1261 cgattagcga aatgcaaact tccatctacg cacgcacagc atttacaaaa tcagccagta
     1321 atccatacgc ggtactttgc agatttggcc ggtgggaaat tatcgtcaat cccggaatca
     1381 tatcgagttc aaaatggacc ccaagtgacg tgccacgaac actaccaagc ggatctgaga
     1441 ccgagacttg ttctggcctt accgaggcaa tgaccttgtc gtcgtctgcg aactttactc
     1501 ttgatacaag cttgaatggc ttcccctccg ctttagccga ttgcaatgtc gcgacatcaa
     1561 gccctcttat tccttccctg cggacgtcgg atggaagcag ttttactttt agaatgaccc
     1621 gggcaattgc gcagacctta acgatcgaat cccagccttc aacatcgtga gacggatcgg
     1681 tttcagtaac gcccagctcc tgcgccattc gcacgccctc gtcaaatgtg cgtcccgtct
     1741 ccatcgtttc cagaatcacg ttagtagttg aattaaaaac tccggtaaag ccccggagtc
     1801 ttgccaacgg caaggcttcg cgaaacaagg aaaagattgg cgcactatcc aacaccgccg
     1861 actcaaagaa atattttttg ccttgagcgt tcgcgatctg ttggagctcg tcgtaagcgt
     1921 agacaatggg agccttattc gcgctgatgg catgcgcccc gtattgcaga gccgctttca
     1981 aataatcgat cgctggttga ccggaatccg ggttcagtga agtggtctcg aacacaacgt
     2041 caggccgcac atgattcaac cactcctgta tacttgattg cgcagtagca tctccaaaat
     2101 ctcccgagac gattttcgtc gcgtcaaaag ccttaactga gctcaaccat ccgagccttc
     2161 tggttgcgac tccatgaatg cgccaatcga tcccataggt ctcgcgaagt tcgctcgatt
     2221 tcgtttgcag caaccgcgcc agcgcacgtc ccacatttcc gaaacccaga aaacaaactc
     2281 tgtactcttt cattcggcac cctttccgtt attatagtcg ccggagtaac ctcgaactga
     2341 tcaagttacc attgcgcgat gaaacaatac cacgatctgc tccaatcaat tttgcaacac
     2401 ggctctgctc atgaagaccg aaccggagtc ggaacaattt ctcacttcgg ttttcaaact
     2461 cgattcgatc ttagccgcgg ctttcccatc gttactacca agcgagttcc ctttcgctgg
     2521 gtcgccgagg aactattctg gtttctctca ggcgatacca acgaagagaa tttgcgcgcg
     2581 cgtggcgtcg atatctgggc tgagtgggcc gacgacgctc ataccgcaaa gtttggcaga
     2641 gagcccggtg accttggacc agtttacggc tatttgtggc gctcgtttgg aggagattat
     2701 cctgagcgca atggtgttga tcagatcgca cggctgatgg accaactttc atcaaacccg
     2761 gactctcgac gtttgattgt caccggttgg gaccctcgcc aggcagataa ggtggacctt
     2821 ccaccgtgtc acacactttt tcagttcaag gttgagaatc ggcaaactct tcattgccag
     2881 ctttatcaac gatcggctga cgctttcctt ggagtgccct ttaacatcag ctcgtacgca
     2941 ctgctgactc acctggtggc tcatgttgct gaccttgaag tgggcgaatt tgtttatacc
     3001 ctgggcgatt accacatcta caagaaccat cttgaacaag tgaatgagct tctgtctcgc
     3061 gagcccctac ctttaccgga gcttaagatt gaggatccgg agaaaaagct ccgcggtctt
     3121 gagggactct taagcgttaa atacgaacat ttaaatttga ttggttacaa gtcgcacgga
     3181 aagatcgcgg ccccggtcgc agtgtgacct tgcaaaaaaa tgatcatcgg aattgcagcc
     3241 gtagaccgac agggagccat cggcaaagat ggtaagttgc cgtggcacta ttcgtctgac
     3301 atgaaattct ttcgtgagac cacgtccgga catgcggtcg tgatgggccg tcgtacgtgg
     3361 ttaactctga ataagccttt aaagaacaga ttaaacattg tactgagtcg cgattcgagt
     3421 attgagccgg ccgagtcgtt gttagtactg cgcgatctgg aatcagtgct ttcgttcaat
     3481 gaatcgctta ccacggatct gttcgtgatc ggcggcgcgc aaatctacaa agcatttcta
     3541 ccgcatatcg aacgatggtt catcactgaa gtgccgttaa cggtaaaagg agctgacacg
     3601 ttcatgccgg agaattattt gaaaggtttt aagaggtatg aaaagcaagg gctggatgag
     3661 gaattgattg ttagtttcta tgaacgaagc tgaacatttt ccatttctca tttctcattt
     3721 ttcattttgc agaaaccaag cgagtacctc gatcgaccat acttaataag acgagcggtg
     3781 gtgttggtca gtaccggatc gcggtagcgt cgggtgactc tcgagctacg tgcagctgga
     3841 tcatgagcct caaaacaatt cctcgagcgc ttcctcaacc gacttcactc caacaatccg
     3901 caccccaagt agtctctcaa gtccactggc gttcgaggcc ggcatcacaa tcttcttgaa
     3961 gccaagtgcc tgaacttcgc gagctcgcac tccagcctgc attgccccgc ggacctcgcc
     4021 ggtcaaacca acctccccaa agactgcggt gtgcggatca accggactgt ttttaaaact
     4081 tgaagctatc gccgccacca ctccaaggtc agctgctggt tcatcgatct ccagaccgcc
     4141 tgcaatgttg ataaatacat cgtcaccgat taactgcagg cctgcgcgct tctccaacat
     4201 agcgatcatc agagacacgc gattttgatc gacgccctgc gtcattcgtc tgccgctacc
     4261 gtactttgag cttgaaacaa gcgcctggat ctccaccagc aacggacggg ttccttccat
     4321 acacgccgta acaattgaac cagca
//
LOCUS       DASSFP010000018         6576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_448713, whole genome shotgun sequence.
ACCESSION   DASSFP010000018 DASSFP010000000
VERSION     DASSFP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448713"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFH91_00410"
     CDS             <1..250
                     /locus_tag="VFH91_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861464.1"
                     /translation="LVTDVSYFSEKPFGPGPTANLPSRIEITRPQDQYKLSITYQDPP
                     SVELNRDYPPTAFVLENKWQLPEVDLDAQNSRKVTANH"
     gene            425..1117
                     /locus_tag="VFH91_00415"
     CDS             425..1117
                     /locus_tag="VFH91_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6861465.1"
                     /translation="MRTILQAENLVKVYRIGKVDVPALRGVSLEVEEGEFLAIMGPSG
                     CGKSTMLHLLGGLLTPTSGKIVIDGDDITSASDAKRTAVRRKKIGFVFQRFNLFPTLT
                     AEGNLRLAERIHGNGGEDPGRRREVLRLLGLEDKLHHKPLELSGGEQQRVALARAVVT
                     RPAIVLADEPTGNLDSENSALVLNMFQELNFRFNQTIIMITHNPEAAAMCKRIVQMRD
                     GHIVEPSPGVRL"
     gene            1204..1554
                     /locus_tag="VFH91_00420"
     CDS             1204..1554
                     /locus_tag="VFH91_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861466.1"
                     /translation="MKKVRGLLTTLKKIFFWNYARNTWQWDILCVVILVFIFLTPKSW
                     FSGGERPVKVEHQNPIAKTLVLSPEVVDNEEDKSKIEQHVRLLTGRSETQVLAVRKMV
                     GSDGKTLSYEVDIR"
     gene            1619..2314
                     /locus_tag="VFH91_00425"
     CDS             1619..2314
                     /locus_tag="VFH91_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET6861467.1"
                     /translation="MKRFVPLVLLSALIFCAAVLAAPKSAKAQGAGLVSSVLSRLEKN
                     RASLKSLKAGITIEKYNSQIGTNDKSEGVVIYLPGSGRQASLRIDWTRPSTETLAVSK
                     GRYQLYRPKWNTVIVGLQNKAPQTSGLIDMMYMSRQQLETRFQPLQDVREETLGGEVS
                     TIHLTLIPKGNAGYKYAEIWIDSAGMPVQTKIVEKNDDSTTMRLTKLQKNVPVSSGEF
                     ELKLDSNVKVVKS"
     gene            2582..3598
                     /locus_tag="VFH91_00430"
     CDS             2582..3598
                     /locus_tag="VFH91_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6861468.1"
                     /translation="MRKTKFLKLKVREASFISRGLLSTRHPVLAHLIPMRRCNLACGY
                     CNEYDHTSKPIDLEIIKSRLNKLADLGTSIVTISGGEPMMHPELDQIINHIRHRGMIA
                     GLISNGYYFTPDRIKRLNRAGLEYLQISIDNVEPDEVSRKSLRVLDKKLRFLSEHADF
                     HVNINSVIGGGIKNPEDALTVAKRAVELGFSTTVGVIHDGDGTLKPLSTKEKDIFQQV
                     KKLGNKDHARLNWFQDSIAEGKTYNWRCRAGARYLYICEEGLVHWCSQQRGYPGIPLE
                     QYTMDDFDREYKTEKWCAPTCTLQCVHQVGILDNWRDPQKTPAQMAQEQKKDKAVGEM
                     IGAD"
     gene            complement(3607..4263)
                     /locus_tag="VFH91_00435"
     CDS             complement(3607..4263)
                     /locus_tag="VFH91_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861469.1"
                     /translation="MSAGEFFDLIRPAAIVLAALISGWVLASARKRFPTYIAFIWTAG
                     TLFLPLIVFPLYLAILLIWRRPARTRKWRVSLPFIYSLVVLSVVGIYFYRDYRSVDAH
                     LARATQARLKSDRGSTISELQSALALEDDAHTHKLLALEFFEVGYLSEAISEYRLAEK
                     GGEPDDSIHFQLGIIFERLNQLGQARLEYQQFLSSSTCLTVNPRCESARHRLREITKQ
                     "
     gene            complement(4281..5720)
                     /gene="pyk"
                     /locus_tag="VFH91_00440"
     CDS             complement(4281..5720)
                     /gene="pyk"
                     /locus_tag="VFH91_00440"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET6861470.1"
                     /translation="MRKAKILITIGPASRDNEVIEKLLNAGANGVRINMSHGTHEEKA
                     ADIALARAAAARIGKPLAILVDLSGPKIRTGKLIGSEPVKLVSGSRFVITNRPIVGDH
                     TQVSTNYPDLPTVVQPGTRLLLDDGAIALLVEETTDTDVICRVVDGGLLGERKGINLP
                     GVDLPIDSLTEKDLVDLNWAVQQKVDYIALSFVRRASDCERAKKLILDAGGHAPLIAK
                     IEKAEAIDRLDEIIAAADGIMVARGDLGVETSVELVPVYQKRIIDKSIKAGKMVITAT
                     QMLQSMVSNPRPTRAEASDVANAVWDSTDCVMLSNETATGDYPVAAVATMARIIESAE
                     SAKEKPLQDMTKWAGKQSGRVSRALCEAAAFASQEMGTTVTVVFTESGLMARRLSALR
                     PEQRIVALTHTDEVVRELSLIWGVEPLITPKADTTEGMLRAGEETLIEAGVVEKGELI
                     VVMAGRLSGLGLSTSVTIFTVEGNLGPTR"
     gene            complement(5795..6466)
                     /locus_tag="VFH91_00445"
     CDS             complement(5795..6466)
                     /locus_tag="VFH91_00445"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6861471.1"
                     /translation="MKTILLRNSSENCHNTRIPSPSILRKKRWRIHGLLAIGIMLAAI
                     FLFPACSSKEVGPDNTTPVASNPPSTTLPMPPLSGNSLSKMGWEMADGRRNVFSDFKG
                     SALVLDFYATWCEPCRASVPHLIALQKKYADQGLKVIGLNVGGPDDLAEVDSFAKELN
                     IDYVLAVPDDDLVHFLLADTSDIPQTFVFDRQGVMARRFIGFGPATADQMESAIQAAL
                     RPSTP"
BASE COUNT         1673 a         1626 c         1649 g         1628 t
ORIGIN      
        1 actcgtaact gatgtatcgt actttagcga gaagccattt ggccctggtc caacagcaaa
       61 tcttccaagt cgcatcgaaa taaccagacc tcaggatcaa tacaaactta gtatcactta
      121 tcaggatccc ccgtccgtag agttaaacag ggactatccg ccgacagcct ttgtgttaga
      181 gaacaagtgg caactacctg aagttgatct cgatgcacaa aactcgcgca aggtaactgc
      241 aaatcattaa ccgtaaggtc gctttttcac tcgtttcctt cgactctgct agataacaac
      301 gttcagatgt attaacatct gtcgcttgag ttttacatca actccatgtg taggtgatcc
      361 cgctgttcgt tagaaccttt cctggccgtg ctgaccaaaa attgcgaacg gccgtcacaa
      421 ttcgatgaga acaattctac aagctgaaaa tctggtgaag gtctatcgta tcggcaaggt
      481 cgatgtgccg gcactgagag gtgtttcact cgaggtcgaa gagggcgaat ttctggcaat
      541 catgggtcct tctggttgtg ggaaatcaac gatgttacac ctgcttggcg gcctcttgac
      601 cccaactagc ggcaaaattg tgattgacgg cgacgacata acgtcagcct ccgatgctaa
      661 aaggactgca gttcgacgca agaagattgg ctttgtattc caaaggttta atctgttccc
      721 aaccttaacc gctgaaggaa atctccgact ggcggagcga attcatggca acggcggaga
      781 ggatccaggg cgtcgtcgag aggtgctaag gctgctgggt ttggaagaca agcttcatca
      841 caagcccctt gaactatcgg gtggcgaaca acagcgtgtc gctctcgcgc gagcggtggt
      901 gacccgacca gcgatcgtcc tggccgatga accaactgga aatctcgatt cggaaaattc
      961 tgccctcgtt cttaacatgt ttcaggaatt gaatttccga ttcaatcaaa ccatcattat
     1021 gattactcac aatcccgagg ccgcggccat gtgcaaacga attgtgcaga tgcgcgacgg
     1081 acacatcgtt gaaccctcgc cgggagttcg cttgtaataa atccgtttac atggtgtagt
     1141 ctaaacgcta gaatctttgg gtacccgcca gccacggagt ctcgtggctg atcacttgca
     1201 agcttgaaaa aggtgcgagg cctgctaacg acgctaaaga agattttctt ctggaactac
     1261 gcaagaaata catggcagtg ggacatactt tgtgtcgtca tcctcgtatt catctttttg
     1321 acgcccaaga gctggttttc tggtggcgaa cggcctgtaa aagttgagca tcaaaacccc
     1381 attgcgaaga ccttggtgct aagcccggaa gtcgttgaca acgaagagga taagagcaaa
     1441 atcgaacagc atgttcgatt gttgaccgga cgttccgaga ctcaggtgtt ggctgtacgg
     1501 aaaatggtcg gttcagacgg aaagactctc agctatgagg ttgacatccg gtagtctttc
     1561 cctataattg ctcggttttt gcgagccgag ggattggtaa tttgatgaag gagtttgtat
     1621 gaagagattt gttcccctgg ttctgctgtc agctctcata ttctgtgcgg cggttttggc
     1681 tgcgccaaag tcagctaaag cacagggcgc tggtctagta agttcagtgc tgagtcggct
     1741 ggagaagaat cgggcgagtt tgaagtcact caaggcgggg ataaccatag aaaagtacaa
     1801 ttcgcagatt ggcaccaacg acaagtcgga aggcgtagtg atctatctac ctggctccgg
     1861 tcgtcaggct tcgctgcgaa tcgattggac acgtccgagc acggagactt tggcagtatc
     1921 gaagggtcgt tatcagctct acaggccaaa gtggaatacc gtcattgttg gtctacaaaa
     1981 taaggctcct cagaccagcg gtctaattga catgatgtac atgtccagac agcagttaga
     2041 aacccgattc caaccactgc aggatgttcg cgaagagaca ttgggtggtg aagttagcac
     2101 gatccatctc acgctgattc ccaaaggaaa tgcaggatat aagtatgctg agatctggat
     2161 cgactctgct ggtatgcccg tacagaccaa gatagtcgag aagaacgacg attcaacaac
     2221 tatgcgtttg acgaaattgc aaaaaaacgt accagtttcg tccggtgaat ttgagctgaa
     2281 gctcgattca aatgtgaagg tcgttaagag ttaaagtacg taagattctt ttgcagcctc
     2341 agagggctct gtaaataaca gagccctttc ttatttttgg gttttttgga gtgcgctggc
     2401 ctggcggcgc tttgttatct cgccctttgt tatataagga cagatctcgg aaagttggcg
     2461 gtctaacaaa gcgccgccgg gccggcgcac tccaaagtca tgtcttgcgt tctcttgttt
     2521 ggtcgaatat gcttcgctgc aaagtttaga aggaccatct ggttttataa ctgttcaact
     2581 gatgcggaaa actaagtttc ttaaactaaa ggtacgcgag gcaagtttca taagccgggg
     2641 cttattgtcg actcgtcatc ctgttcttgc tcatctgatc ccgatgcgtc gctgcaatct
     2701 tgcctgtggc tattgcaacg agtacgacca cacgtccaaa ccgattgatc tcgaaatcat
     2761 caagagtcgt ttgaataagc tggcggacct cggaacttcg attgtgacca tttcaggtgg
     2821 cgagcccatg atgcatcccg agctcgatca gatcatcaat catattcgac accgaggaat
     2881 gatcgccggc ctaatctcga acggttatta cttcacacca gacagaatca aacgcttgaa
     2941 tagagcgggc cttgagtatc ttcagatcag catcgacaac gtcgagcccg acgaggtttc
     3001 gcgcaagagc ctgcgtgtgc tcgataagaa gttgcgcttc ctgtcagaac atgcagattt
     3061 ccatgtcaac atcaactcgg tgattggtgg tggaataaag aatccggaag acgctttgac
     3121 tgtcgctaaa cgtgcagtcg aactaggttt ctcaacgacc gtaggcgtga tccacgatgg
     3181 ggatggcact ctcaagccgt tatctacaaa agagaaggat atcttccagc aggtcaagaa
     3241 actcggtaac aaggaccatg cacgtctaaa ctggtttcag gacagcatcg ctgagggtaa
     3301 gacgtataac tggcgctgca gagccggcgc tcgctatcta tacatttgcg aagaagggct
     3361 cgttcactgg tgctcgcaac aacgaggtta tcctggcatt cccctcgagc agtacacgat
     3421 ggatgacttc gatcgcgagt acaaaaccga aaaatggtgc gcgccgactt gcacccttca
     3481 gtgcgtacac caggttggaa tactcgataa ctggcgcgat ccacaaaaga ctccggcgca
     3541 aatggcccag gagcaaaaaa aggacaaggc cgtcggtgaa atgatcggcg ctgattagaa
     3601 agagaattac tgttttgtga tctctcgtag tcggtgtcgc gccgactcac aacgtggatt
     3661 aacggtcaga caggtcgaac tcgacaagaa ctgctgatac tcaagccgag cctgtcccag
     3721 ctgattcagc cgctcaaata tgattccgag ctgaaagtga attgaatcgt caggctcgcc
     3781 gcccttctcc gccaaacggt attcagaaat agcttctgac aaataaccaa cctcaaagaa
     3841 ttcaagtgcc aacaacttgt gtgtgtgtgc gtcgtcttca agcgccagcg cactttggag
     3901 ttcagaaatc gtactgccgc gatcagattt gagtcttgct tgagtagcgc gggcgagatg
     3961 agcgtccaca cttctgtaat ccctgtaaaa atagatgccc accacagaca agacaactaa
     4021 cgaatagatg aatggtaaac tcactctcca tttccgagtt ctggccggcc ggcgccaaat
     4081 tagaagtatc gctagataga gtggaaaaac gattaaagga aggaaaagcg ttccagccgt
     4141 ccagataaat gcaatgtaag ttgggaaccg ttttcgagcg gaagctaaca cccaaccgga
     4201 aataagtgcg gctaaaacaa ttgcggcagg tcggatgaga tcaaagaact cacccgctga
     4261 catgtcgata cgcaaagtca ctaacgtgtc gggcccagat tgccttcaac agtaaaaatg
     4321 gtgaccgagg ttgagagtcc aaggccggaa agtcggcctg ccataactac aatcaattcg
     4381 cccttttcaa caactccggc ttcgatcaga gtttcctcgc cagctcgcaa catgccctcg
     4441 gttgtgtcag ccttgggagt gatcagaggt tcgactcccc agatcaacga aagttctcgg
     4501 accacctcat ccgtgtgagt caaagcaacg atcctttgtt caggccgcaa tgccgaaagt
     4561 cttcgcgcca tcaaaccgga ctcggtgaac actactgtga ctgtggttcc catctcctgg
     4621 gatgcaaagg cggcggcctc acagagtgca cggctcacgc gaccagattg cttgccggcc
     4681 cacttcgtca tgtcctgaag tggtttctcc ttggcgctct cagctgactc aatgatgcgt
     4741 gccatggttg ccacagcggc aacaggatag tcacccgttg cagtctcatt cgacagcatg
     4801 acgcaatcgg tgctatccca cacggcattt gcgacgtccg atgcttcggc tcgagtggga
     4861 cgggggttcg agaccatcga ttgcagcatt tgtgttgcgg tgatcaccat ctttccggct
     4921 ttgatagact tgtcgatgat ccgcttttga taaacaggaa cgagttcaac gctggtttca
     4981 actccaagat ctccacgcgc caccatgatc ccgtccgcag ctgcaattat ctcgtcgaga
     5041 cgatcgatcg cctcggcctt ctcgattttc gcgatcaaag gcgcatgtcc gcccgcgtcc
     5101 aggatcaatt ttttagcgcg ttcgcaatca cttgctcgac gtacaaaaga gagcgcgatg
     5161 tagtcgacct tttgttgaac cgcccaatta agatcgacaa ggtccttctc agtaagtgaa
     5221 tcgattggaa gatcgactcc aggcaggttg attcctttgc gttcacctag cagtccgcca
     5281 tccactacac gacaaatcac gtctgtatca gttgtttcct caaccagaag tgcgattgcc
     5341 ccatcgtcca acagtaaacg tgtaccaggc tgaactaccg ttggaaggtc aggatagttt
     5401 gttgatactt gagtatggtc gccgactatt ggtcggtttg tgatgacgaa tcttgatcct
     5461 gaaaccagtt tgactggttc actgccaata agttttccag tccgaatttt tggcccggat
     5521 agatcgacga gaatcgcaag tggttttcca attcttgccg cagcagcacg agccagggca
     5581 atgtcagcgg ccttctcttc gtgagtgccg tgcgacatgt taatacggac accatttgcg
     5641 cccgcgttca gtagcttttc gattacttca ttatctctcg aagctggacc aatagttatt
     5701 agtatctttg cttttctcaa agttcgaccc gtgactgcct ttcgttaaaa ttcggggatt
     5761 agtttcctaa gttagtcgtt ctcaaaggtg tatatcaagg agttgacggt cgcaacgcag
     5821 cctgaatagc actttccatt tgatctgctg tagccggacc gaacccgatg aaacgtcggg
     5881 ccatgactcc ctgcctgtcg aaaacaaagg tctgaggaat gtcacttgta tcagcgagca
     5941 gaaaatggac cagatcatcg tcgggcaccg ccaacacata gtcgatatta agttcttttg
     6001 caaagctgtc gacttcagcc aaatcgtctg gacctcctac attcagccct atcactttca
     6061 aaccctggtc ggcatatttt ttttgcaatg caattaaatg tggaacactt gctcgacagg
     6121 gttcgcacca ggtggcgtaa aagtccagga ccagtgccga ccctttgaag tcggaaaaaa
     6181 cattccggcg accatcggcc atctcccagc ccatcttgct cagtgaattg ccacttagtg
     6241 gaggcattgg caatgtagta ctgggaggat tcgaagctac cggtgttgtg ttgtctggac
     6301 caacttcctt cgagctacaa gcgggaaaga gaaatatggc ggccaacatg atgccgattg
     6361 caagcaagcc atgaattcgc catcttttct ttcgcaaaat tgatggagag ggaattctgg
     6421 tgttgtggca gttttcagac gagttgcgca ggagaatcgt tttcattgct cctcgtcagt
     6481 ttggtcatgg tcatttgccg cagtggtttg tggttggtcc tgattgaccc gctcgccggg
     6541 cacacggtat tcgccctcaa cccagcgacc gaggtc
//
LOCUS       DASSFP010000019         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_3594, whole genome shotgun sequence.
ACCESSION   DASSFP010000019 DASSFP010000000
VERSION     DASSFP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3594"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..884
                     /locus_tag="VFH91_00450"
     CDS             345..884
                     /locus_tag="VFH91_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6861472.1"
                     /translation="MSSVENFSNEIAVGDAFVGHARNFLIHHYLPKIERCLEKLTDDQ
                     IWWRPNADSNSIGNLVLHLCGNARQWIIAGLGGTEDTRKRDIEFQQKTVIPRSQLETL
                     LRSTLLEIDHVLTNLDPSTLLEVRRIQESDVNVLEAIFHVTEHFSMHTGQIIMMTKML
                     TKSDLAFYDFSTGAPVHSW"
     gene            1010..1288
                     /locus_tag="VFH91_00455"
     CDS             1010..1288
                     /locus_tag="VFH91_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET6861473.1"
                     /translation="MTYVVAEPCVNCRYTDCALVCPVEAFHLDDDMLVINPETCIDCD
                     ACVPECPVEAVFPEAGVPAEWSHFTGINAERSLSGLPTITARLDSLCE"
     gene            1294..1611
                     /locus_tag="VFH91_00460"
     CDS             1294..1611
                     /locus_tag="VFH91_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861474.1"
                     /translation="MKKRTRWLIGGLAIAASALGAATAYFKKSKMSSRKHFKQIGDVW
                     ARPGMMVTFRAELMPGRERSERTFRVKELFSSGRVSLHGVDGEHVENEFEPIQPDRPL
                     AVS"
     gene            complement(1613..2389)
                     /locus_tag="VFH91_00465"
     CDS             complement(1613..2389)
                     /locus_tag="VFH91_00465"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HET6861475.1"
                     /translation="MRIQLLPSTFDEVGQATLDQRLTCFLIDDCVAVDAGSIAIALTD
                     EQRAAVRDIIVTHPHMDHIASLPIFIDDLYPTLTQPVRIHATPEVIGLLERDVFNWNV
                     YPRFSDLKNDFGPVMEYVPIPPDGVFTVGHLKAIAVPVNHIVPTVGLILSDNKTTVAF
                     SSDTAETDEFWKIVNEIGHVDALLIEASFPNEMANLAEVSRHFTPASLGKELKKLSHD
                     GIDILAVHLKPSYRETIVRQLKELNLPNLSVMEPGKPYNW"
     gene            complement(2482..>3589)
                     /locus_tag="VFH91_00470"
     CDS             complement(2482..>3589)
                     /locus_tag="VFH91_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET6861476.1"
                     /translation="VLTLVLGIIAILIASLLGLLVLLVIGVETGPVPFILGLFSAIIP
                     VPLYVSLVLWIDRYESEPPWMLATAFFWGVLVAPFFSFLLNTLGSILVTVLAGKQAGE
                     TFALVISAPIVEESSKAAILIIFYFWKKEEFDGVVDGIVYASLVALGFAMTENILYYG
                     KAALEHGAAGLTLTFIVRGAFAPFSHPLFTSLTGIGLGISRQTNNLVVKLATPVIGFL
                     MAVFMHSIWNGSGALGGGGIFILTYLIVMVPAFVVMIVVISLALRREGEVVRQFLLPE
                     FQKGLFSDDEYRQLGSIGGRMSSSFSALSRRGVKGWRTRRQFNQMASELAFHRSRVSR
                     GLHSSEHDAIAMEAAYLAALKSLIEDVRSVQSRR"
BASE COUNT          942 a          897 c          910 g          840 t
ORIGIN      
        1 aggcctaatt caagtccaaa aagccccagt tatcaaatgc tggggccctt cttgccacac
       61 tctccgcgac cttaatacac agaatggttt tggttgacag ccgactttcg gcgggactag
      121 catccgtcta ctttccaaac cgaaggcggg cttatgccaa ggaagagttc gcacaaacca
      181 aaaaagagaa tcgaggcgcg agccattttt gagagcattc gcaaaccgaa tgctccgccg
      241 gggcaccctc tgtctcgcgc aaaaccggaa gagatagcgc gcccctccca gcgaaaagca
      301 aagcacaaac gacctccatc agatagtggt gaagaggatc tctgatgtcc tccgtcgaaa
      361 atttctcaaa tgagattgct gtgggtgacg ctttcgtcgg ccacgctcgg aattttctca
      421 ttcaccacta tctgccgaaa atcgaacgtt gcctggaaaa gctcactgac gatcaaatct
      481 ggtggcggcc caatgctgac tcaaacagca tcggaaatct ggtattacac ctctgcggaa
      541 atgcgaggca gtggattatc gcaggcttag gtggaacgga ggatactcgg aagcgagaca
      601 tagagttcca gcaaaagacg gtaataccgc ggagccaact tgaaaccttg ttacgttcta
      661 cgctgcttga aatagatcac gtgcttacga atctggatcc ctcaactttg ctggaagtgc
      721 gccgtattca ggaatcggac gtaaacgtgc ttgaggcgat attccatgtg actgagcatt
      781 tttccatgca taccggtcaa atcatcatga tgacaaaaat gctgaccaag tcggacctcg
      841 cgttctacga cttctcaacc ggagctccag ttcacagttg gtagtgacaa atagagagaa
      901 ttctgagatc ttcctaaaac ttgctcttcg cgtttaatat aagttgtgtt ccagttcgac
      961 actgttcgac gtaagattct ttgtccgcgc cgaggaggca tatagccaga tgacctacgt
     1021 tgttgccgaa ccttgtgtta attgccgcta cacagattgt gctttggtat gtcccgtcga
     1081 agcctttcac ctggacgatg acatgctcgt gattaatccc gaaacctgta tcgattgcga
     1141 tgcttgcgtt cccgagtgcc cggtagaagc tgtctttcct gaggccggcg tgccagcgga
     1201 gtggtcacac ttcacgggga tcaatgctga acgatctcta agtggccttc cgaccataac
     1261 agctcgtttg gattcgcttt gtgaatagta ttcatgaaaa agcgcaccag gtggcttatc
     1321 ggaggtttag cgatagccgc aagtgcgcta ggcgccgcga ccgcctactt caaaaagagc
     1381 aagatgtcat cgcgcaaaca ttttaagcag attggggacg tttgggcccg accaggaatg
     1441 atggttacgt ttcgtgccga attaatgcct ggccgtgagc gctcggaacg gacgtttcgc
     1501 gtgaaagaac tcttctcgag cggacgtgtt tcattgcacg gagtcgatgg ggaacacgtt
     1561 gaaaatgagt ttgaacctat tcagccagat cgtccactcg ccgtaagcta acctaccagt
     1621 tgtaaggctt tcctggttcc atcacactca aatttggcaa gtttaattct ttgagttgcc
     1681 gcactatcgt ctctctataa gatggtttga ggtgaacagc gagaatgtcg ataccatcgt
     1741 gtgaaagttt cttcagttct ttgccgagcg aggctggtgt gaaatggcga gagacctcag
     1801 ccaggttggc catctcattt gggaacgaag cctcaattaa gagcgcatcg acatggccaa
     1861 tctcgttaac aattttccag aactcgtcgg tttcagctgt gtctgaactg aatgccacgg
     1921 tcgtcttatt atccgacaat atgagaccta ctgtcggaac gatgtgattg acgggtaccg
     1981 caatcgcttt caggtgtccg acagtgaaaa ctccatcggg gggaataggt acatattcca
     2041 tcactggacc aaaatcgttt ttaaggtccg agaaccgcgg atacacattc cagttgaaga
     2101 cgtcccgctc cagcagtcct ataacttctg gtgttgcatg aattcgcaca ggctgagtta
     2161 gagtgggata gaggtcgtcg ataaagattg gcagactcgc gatgtggtcc atgtgcgggt
     2221 gagtgacaat gatgtctctg accgcggctc gctgctcatc ggttagagcg attgcgatac
     2281 ttcccgcgtc gacagctacg cagtcgtcga tgaggaagca ggttaagcgt tggtccaatg
     2341 tcgcctggcc cacttcatcg aaagtgctgg gtaagagttg gattctcaca atgctcagct
     2401 cagttgaaca tccgtggcgc gggtcaagta tatgctagaa gcactaacgc cgcgataata
     2461 aaatttccgt tgcggatccg cttaacgacg cgattgaacg ctacgtacgt cttctataag
     2521 acttttaaga gcagccagat aagcagcctc catggcgatt gcatcgtgtt cagatgaatg
     2581 caatccgcga gagacacggc tgcgatgaaa cgccagttca cttgccattt gattaaattg
     2641 ccgtcgcgtc cgccagcctt tgacaccgcg gcgtgacagg gcagagaacg acgaactcat
     2701 tcggccgccg attgaaccaa gttgcctgta ctcgtcgtct gagaaaaggc ccttttgaaa
     2761 ttcaggaagc agaaactgtc gtactacttc gccctcacgt cgtaaggcca gagagatcac
     2821 gacgatcatg actacaaaag ctgggaccat cactattaga tacgtcagaa taaaaatccc
     2881 tccaccaccg agcgcgccgg atccgttcca gatagagtgc atgaagaccg ccattaagaa
     2941 tccgatcact ggcgttgcga gttttacaac aagattattg gtctgacggg aaattcctaa
     3001 gccgataccg gttagactgg tgaacaaagg atgtgaaaag ggtgcaaatg cgccgcgaac
     3061 gataaacgtt aatgttagac cagccgcgcc gtgttcaagg gcagcttttc cgtaatacag
     3121 gatattttct gtcatcgcga atcctaaggc gacgagcgaa gcgtaaacga ttccatctac
     3181 cacaccatcg aactcttctt ttttccaaaa gtaaaaaata atcagaatcg ctgccttgga
     3241 actctcttca acaatcggtg ccgaaattac aagcgcaaac gtttcgcccg cttgtttccc
     3301 cgcaagaacc gtaaccagga tgctgcctaa ggtgtttaga agaaacgaga agaatggcgc
     3361 aacaaggact ccccagaaga aagcagtggc gagcatccac ggcggttcac tttcgtaacg
     3421 gtctatccaa agcaccagcg acacgtacag gggtaccgga atgatcgcgc tgaagagtcc
     3481 caggatgaac gggacgggtc cggtctccac gccgatcaca agcaggacca acaacccgag
     3541 gagtgaggcg attaagatgg cgataatgcc aaggaccagc gtcagcact
//
LOCUS       DASSFP010000020         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1790975, whole genome shotgun sequence.
ACCESSION   DASSFP010000020 DASSFP010000000
VERSION     DASSFP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790975"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFH91_00475"
     CDS             <1..1345
                     /locus_tag="VFH91_00475"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HET6861477.1"
                     /translation="WPEAIGVAISFEGDSFDARALLINSPGDRSNPIPFLPMFIPGPA
                     IAPESPNILPSDTELFISLSLDLPQIYTRIATLPSPGQMSYNGGTTRKVNEGDTPFST
                     LETQLKIKIKDDLLPLLGSEIALSLPIQTLDFIAPSARRAPAQSPGAQNGAKSQQPFV
                     PGPIVSISLRDREGMKALLPRIVESFGFKGAGAFAQTETREDTEIVSYANTFAYAFIG
                     NFLVLSSDAATIRHVVDSYLKHETLVSDNQFKNYTRWQPRQLQGQVYVSPALMASYRT
                     WAEQLNTVISDQTREFLSRLTLIAEPITYSLSNDGMGAFHELHVPKNLVLMAVTAIAG
                     EANPPPLLNNERTTISALYAVFSAEVSFHTNKGAGTFTSLEDLLDQQLVNKELLENFG
                     YKIEVIASGTKFQATAVPLEYGKTGKRSFFIDESGVVRGGDHAGGAATVEDDPIN"
     gene            complement(1350..>2864)
                     /locus_tag="VFH91_00480"
     CDS             complement(1350..>2864)
                     /locus_tag="VFH91_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6861478.1"
                     /translation="TIVSLQLDDNPDCGTVLVQGNDFYASPKISPDGSQLAWLTWNHP
                     NMPWDGCELWVGDFAEDGTLVSTRWVAGGAAESIFQPEWSPDGVLYFASDRNGWWNLQ
                     RISAEGEIESINHSKAELGMPQWVFGMSSYTFVSPTMVACCPVEQGISRLETIDLTTG
                     TVSNIDCPFTDIQYLRAEGQRLIMRAGAPTDVASISLYDFETGKFETLRRANDQEVYP
                     SYFSIPRPIEFPTEAGLTAHGFFYPPQNPDYRGPDNEKPPLIVKSHGGPTAAASTALA
                     LSIQYWTSRGFAVLDVNYGGSTGYGRQYRERLNRKWGIVDVDDCANGARYLVDKGEVD
                     GKRLIITGGSAGGYTTLSALTFRDRFSAGASHFGVSDLEALAKETHKYESRYLDHLLG
                     PYPARRKIYFERSPINFPEKLSCPVIFFQGLEDKVVPPNQAEKMVDAMRSRGITVSYV
                     AFPGEQHGFRQASNIKRALEGELYFYSRVFNFELAEEVEPIKIENLAKLRRNSR"
BASE COUNT          721 a          767 c          694 g          682 t
ORIGIN      
        1 atggcccgag gcgatcggag tggcaatctc atttgaaggt gactcgtttg atgctcgagc
       61 gctcttgata aactcgcccg gcgacaggag caacccgatt ccctttctgc cgatgttcat
      121 tccgggtccg gcgatagccc cagagtcacc caatatttta ccctctgaca ctgaactctt
      181 catttcgctg tcactcgact taccacagat ctacacgcgt attgccactc ttccttcgcc
      241 cggtcagatg tcctacaacg gcggaacgac cagaaaagtg aacgaaggcg acacgccgtt
      301 cagcactctt gagacacaat taaagataaa gataaaagat gatctattgc cgttgttagg
      361 ttccgaaata gcactcagcc tgccaattca aactttggac ttcatcgcgc catcagccag
      421 aagagccccc gctcaatcac caggcgcaca gaacggagca aaatcacaac aaccgttcgt
      481 tccaggccca atcgtttcga tctccttacg agatcgcgaa ggaatgaaag cactcctccc
      541 caggattgtt gagtcgtttg gtttcaaagg cgccggcgct tttgctcaaa ctgaaactcg
      601 agaagacact gagatcgtct cgtacgctaa cacttttgct tatgccttta ttggaaactt
      661 ccttgtgctg tcgtcagatg ccgcaaccat ccggcacgtg gtggacagtt acctcaaaca
      721 cgaaacctta gtaagtgata accaattcaa gaactacaca cgctggcaac cccgccagtt
      781 gcagggtcag gtctatgttt caccggcctt aatggcgagt tacaggacct gggcggaaca
      841 actgaacaca gttattagcg atcaaaccag ggaatttcta tcgcggctca ccctgatcgc
      901 tgagcccatc acatattcac tctcgaatga tgggatgggc gcatttcacg aactacatgt
      961 accaaagaat cttgtgttaa tggccgtgac agcaatcgcc ggagaagcta atccgcctcc
     1021 actattgaac aatgaaagaa ccacgattag cgcactctac gcagtttttt cggcagaagt
     1081 atcttttcac actaacaagg gcgccggaac ttttacgtca cttgaagacc ttttggacca
     1141 gcagttggta aataaagaac tactggaaaa cttcggctat aagattgagg tcatcgcgtc
     1201 aggaacaaaa tttcaggcga ccgcagtccc tctcgagtac ggaaagaccg gtaaacgatc
     1261 gtttttcatt gatgagagcg gagtagtgcg cggcggagat catgccggag gcgcagctac
     1321 ggtcgaagac gaccccatca attagatcgt cagcgagaat ttcgtcgcaa cttcgcgagg
     1381 ttctctattt ttatcggctc tacctcttcg gccaactcga agttgaatac tcgtgaatag
     1441 aagtatagtt ctccttcaag agctcgcttg atgttggaag cttgacggaa accgtgctgc
     1501 tcgcctggaa aagcgacata tgacacggta attcctctcg agcgcatggc gtcaaccatc
     1561 ttctcggcct ggtttggcgg caccactttg tcttccagtc cctgaaagaa aattaccggg
     1621 cacgacagtt tctcaggaaa gtttattggc gagcgttcga aataaatttt tcgcctcgcg
     1681 ggatatggcc cgagtaagtg gtccaggtat cgtgattcat atttgtgcgt ttctttcgcc
     1741 aatgcctcga ggtcgcttac tccaaagtga ctcgcaccgg cactgaatcg atcgcgaaaa
     1801 gttagtgcag agagagttgt atacccaccg gcgctaccac ccgtaataat taatcgtttg
     1861 ccgtcaacct ctcctttgtc tacaaggtag cgtgccccat tagcgcaatc gtcaacgtca
     1921 acgatgcccc atttacgatt taatcgttcc cgatattgtc gtccgtaacc tgtgcttcct
     1981 ccgtagttga catcgagcac agcaaaaccc cggctggtcc aatattgaat ggacagggca
     2041 agtgccgtcg acgctgccgc ggttggtcca ccgtgactct tgacgatcag cggcggtttt
     2101 tcgttgtcgg gaccacgata gtccgggttc tgaggcggat agaaaaagcc gtgtgccgtt
     2161 agccctgctt cagttggaaa ttcgattgga cgaggtatcg agaaatagga cgggtacact
     2221 tcctgatcat tggcccgtct tagagtttca aactttccgg tctcgaagtc gtacaaactg
     2281 atagatgcca cgtcggtcgg cgcaccagcc cgcatgatga gacgttgtcc ctcagctcgc
     2341 aaatattgaa tgtcagtaaa aggacagtcg atgttggaaa cagtgccggt cgtcaagtca
     2401 atcgtctcga gtcgcgagat accctgctca accggacagc aggcaaccat cgtgggtgaa
     2461 acaaaagtgt atgaagacat gccgaagacc cattgaggca tcccgagttc ggctttggag
     2521 tgattgatgc tttcaatctc gccctcggca gaaatgcgtt gtaggttcca ccaaccattt
     2581 cggtctgaag cgaagtacaa aacgccatca ggtgaccatt caggctgaaa gatcgactca
     2641 gccgctcctc cggccaccca tctggttgag accagagtgc cgtcttcagc aaagtctccg
     2701 acccataact cacaaccatc ccagggcata ttgggatggt tccaggttaa ccacgccagc
     2761 tgcgatccat ctggactgat cttcggcgaa gcgtaaaagt cgtttccctg gaccagtacg
     2821 gtgccgcaat ccgggttgtc gtcaagctga agcgagacga tcgt
//
LOCUS       DASSFP010000021         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1642711, whole genome shotgun sequence.
ACCESSION   DASSFP010000021 DASSFP010000000
VERSION     DASSFP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642711"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..2473
                     /gene="lptC"
                     /locus_tag="VFH91_00485"
     CDS             176..2473
                     /gene="lptC"
                     /locus_tag="VFH91_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET6861479.1"
                     /translation="MQEVARKRAFGIGLRARFPLLAKIAAVVLLCSGLALVAISYYKL
                     RDKRWKSKPGTPELSKNVTGVVEGYEQRVTKNDRLYLLVKASRDITYSDSHHELEAVS
                     VAVYPDVGDVPDQITASRAVYLPDSSIITFQGNVKIETKEKLRVNTETLVFDQTHKIA
                     NTDAPLSFDKDTVKGTSVGAVVDQNTKRLDLKNSVAITVTPKPDKGSKTPGPTPATIK
                     SAQASFEQLLMKLSFSGGVTVEQNRDIMSGDTIYANLNQKQQLEKVEVRGNAYLRAME
                     PGNSAEVHSVNMDFYLDGNQLLQRAVGWDDVIARSLDADSDMQLNAGNIEVNFQARDG
                     VSLLTEMKALGKSVVTLSAPKSKANDPRAASKRLTGDSVKLTWRASGKDLERAEVTGG
                     AELFVEPVVKNAVAERKTITAQQFNCDFFEAGNLTKSCKANVNSKAVLDPVMPTPNRG
                     TRTITAQNMAANFTRETQDIERLDAQGDSKFNENDRNGIAENVSYTSGDQMVRLRGGD
                     PTVWDSRARTKGVELDSDLANDVSYGRGKIATTYYSQEQTNGATPFTKVKSPVFVTSD
                     RAEFRHESGLGIYTGNARAWQDDNFVKGDKLSIYMNEKRMEGVGHIQTALYNAKRKID
                     GASSVVPVFAVSDSMFYSDADRLIHYDGNVDIKQSPDRLTGGVADVYLAKETNDMERT
                     IAQRNVVLTQPSRRGTGDWVEYTAATEVAVLKGNPAKVQDAEKGDTLGERLTLSVRDS
                     IVTADDTRGPQSPGRIRSTHKIKKP"
     gene            2486..3304
                     /gene="lptB"
                     /locus_tag="VFH91_00490"
     CDS             2486..3304
                     /gene="lptB"
                     /locus_tag="VFH91_00490"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET6861480.1"
                     /translation="MTVDQLQLEERTATETSTTSSSSLQPTVDKPNTTSLRADQLVKN
                     YGKRRVVDDVSLQVEKGEVVGLLGANGAGKTTTFYMIVGLERPDGGRVLLGDKDLTRL
                     PMYLRARLGIGYLPQEPSVFRKMTTAQNILAVLETMGLRRREQLKRLEELLEEFGISH
                     VRNTRGDALSGGERRRTEIARALATEPEYILLDEPFAGIDPKAVDDIQNVIIYLRDRG
                     IGILITDHNVRETLGVTDRAYIMAEGKIFRAGKPKDLTDDSEVRRLYLGEKFRM"
BASE COUNT          996 a          873 c          910 g          765 t
ORIGIN      
        1 ccagctgacg agagtaatct attaatacca gacggccact aaccctccct tctgacaaaa
       61 cagcgaacgc cgataactgg tagcgcatcg aactcgcctg atgcgcgtgt tataaacctt
      121 gatgacaaag gaccttaagg gtttgtagat tactgcgggt atactgagtc cataaatgca
      181 ggaagtagca agaaaacgag cttttggtat cgggttgcga gcccgatttc cactattagc
      241 taaaatcgcg gcagttgtcc tgctctgctc gggcctcgcc ctggtcgcga tctcttacta
      301 caagctccga gacaaacgct ggaagtcaaa gccgggtacc cctgagcttt cgaagaacgt
      361 tactggcgtt gttgagggtt atgagcaaag agttactaag aatgatcgcc tttatctctt
      421 agtcaaagcg tcgcgtgata tcacctattc ggacagtcac catgagttag aagctgttag
      481 cgtagcggtc tatccggacg ttggtgacgt gcctgaccag ataaccgcaa gtcgagcagt
      541 ttatctgccc gacagctcta taattacgtt tcagggaaac gtaaagatcg aaacaaaaga
      601 aaagctaaga gttaatactg aaacgctcgt cttcgatcaa acccacaaga ttgcgaacac
      661 agacgcgcct ctgtcattcg ataaggacac tgttaaagga acatcagtag gcgctgtcgt
      721 ggaccaaaac acaaaacgcc tcgacctgaa gaatagtgtt gcgatcaccg taacaccaaa
      781 acctgacaaa ggttcaaaga cgccaggtcc gacccctgcg acgataaagt ctgcgcaagc
      841 ttccttcgag cagctgctaa tgaagctttc attttccggt ggagtcactg ttgaacagaa
      901 ccgcgacatc atgagcggag atactatcta tgcgaatctc aaccagaagc aacaactcga
      961 aaaggtcgag gtgcgaggca atgcttattt gcgcgcgatg gagcccggca attcagccga
     1021 agttcactca gtgaacatgg acttctacct tgacggcaat caactgttgc agcgggctgt
     1081 cggatgggat gatgtgatcg cacgaagtct cgacgccgat tccgacatgc aacttaacgc
     1141 cggcaatatt gaagttaatt ttcaagccag ggacggcgta agcttactta ctgagatgaa
     1201 agcgttgggc aaaagcgtag tgactctttc agcgcccaag tctaaggcga atgatccgag
     1261 agccgcctct aagcgcctca ccggagattc cgtgaaattg acctggcgcg cttcagggaa
     1321 agacctcgag cgagctgaag taaccggagg cgccgaatta tttgtcgaac cggtagttaa
     1381 aaatgcagtg gctgaacgca aaaccattac ggcccaacag ttcaactgcg attttttcga
     1441 ggccggcaat ctcaccaaat cctgcaaggc caacgttaat tcaaaggccg ttctcgatcc
     1501 ggtgatgcca acaccaaaca ggggtacaag aacgatcacc gcccagaata tggccgcaaa
     1561 ctttacgaga gagacgcagg atatcgagcg attggatgcg caaggcgata gcaagtttaa
     1621 tgaaaatgac cgcaatggca tcgcggaaaa tgtgtcgtat acctcaggag atcagatggt
     1681 caggctccga ggtggtgatc caacagtctg ggattctcga gctcggacca aaggagtgga
     1741 gttagactca gacctcgcca atgacgtctc ttatggacgt ggcaagatcg ccaccaccta
     1801 ctacagtcag gaacagacaa atggggccac accattcact aaagttaaga gtccagtgtt
     1861 tgtaaccagt gatcgggccg agtttcgcca tgagagtgga ctcggtatct acactggaaa
     1921 tgcgcgtgca tggcaggacg acaactttgt taaaggcgat aagctttcaa tttatatgaa
     1981 cgaaaaacgt atggagggtg tgggacacat tcagaccgcc ctctacaatg ctaagcgtaa
     2041 gatcgatgga gcctcgtcgg ttgttcctgt cttcgcagtc tccgactcaa tgttttactc
     2101 agatgccgat cgtctaattc attacgatgg aaacgtggac attaagcaga gtccggatcg
     2161 tttgacaggt ggcgttgccg atgtctatct cgcaaaagag actaacgata tggaaaggac
     2221 catcgcgcaa cggaacgtag tgctcacgca accatccaga cgtggcacgg gcgattgggt
     2281 ggaatatact gcagcgacag aagtagcagt cctgaaaggt aatccggcca aggtgcagga
     2341 tgcagagaag ggggatacgc tgggcgaacg attgacgctg tcagtcagag acagtattgt
     2401 caccgccgat gacacgcgtg gaccacaatc acctggtcgc ataagatcga cacataagat
     2461 caagaagcca tagcagctcc atccgatgac tgtagatcaa ttgcaactcg aagaacgaac
     2521 cgccacagaa acttcgacca cctcgtcaag ttccttgcaa cctacggttg ataaaccgaa
     2581 cacaacatcg cttcgtgcgg atcagctcgt caagaactac ggcaaacgcc gagttgttga
     2641 cgacgtcagt cttcaagttg aaaaaggaga agtggttggt ctactgggag cgaacggtgc
     2701 cggtaagacc actacctttt acatgatcgt cggtctggag cggcccgatg gtggccgagt
     2761 gctgctcggc gataaggatc tcaccagact gcccatgtac cttcgcgcca gacttgggat
     2821 cggatacctg ccgcaggagc cctcagtgtt tcgcaagatg acgacggctc aaaatatcct
     2881 ggcagtctta gaaacgatgg gcctgcgacg gcgcgaacag ctcaagcggt tggaggagct
     2941 gctcgaggaa ttcgggatca gtcacgtgcg aaacactcgc ggcgacgcat tatcaggcgg
     3001 tgagcgacgc cgaactgaaa ttgcaagggc tcttgctact gagccagagt acattctgct
     3061 tgacgagcct tttgccggca tcgatcccaa ggcagtggac gacattcaaa atgtaataat
     3121 ttatttacga gatcgtggca taggtattct aatcaccgat cacaacgtac gcgagacact
     3181 aggtgtcaca gatcgggcct acataatggc agaaggaaag attttccggg cgggaaaacc
     3241 aaaagatctc acagacgatt ctgaagttcg aagactttac ctcggcgaga agttccgaat
     3301 gtgaaacccc tacgcacaaa ccatgccgga tcggttataa tctctcccgc gcactttcat
     3361 aagtgcaaaa attacggaat caaaagcagt ttttagttcg ataaatgtct cttaaattaa
     3421 ccaccagctt atcccaacga ctagtgctga ctccgcagtt gcggcaacgc attgagatgt
     3481 tgcagatgac atctcttgag ttgaccgacc ttatccagca acagttgttg gagaaccctg
     3541 tact
//
LOCUS       DASSFP010000022         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1940711, whole genome shotgun sequence.
ACCESSION   DASSFP010000022 DASSFP010000000
VERSION     DASSFP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940711"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFH91_00495"
     CDS             complement(<1..863)
                     /locus_tag="VFH91_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET6861481.1"
                     /translation="MPAKQAAKKTERPERVFLIDSMSHIFRAFYAPMQNRAAPLATAQ
                     GQVTQAVYIFTNMLIKLLREEQPDYIAAIFESREKTFRHETFADYKANREEMPGDLSS
                     QLPYIFRLCDAFNIPVINSPGFEADDVIGALAHRIAEQELQTVIVSNDKDMCQLVKDP
                     YIICMRQNSQVVKRKEPVPPIEWCDEAWVEKKFGVTADKLVDLLGLMGDSIDNIPGAP
                     GIGSKGAVQIIQQFGSIEAALNGWEAVKHKTYRESLRDNAELIRRSRELARIRTDLEI
                     PFNLEDIKARPP"
     gene            complement(918..2831)
                     /locus_tag="VFH91_00500"
     CDS             complement(918..2831)
                     /locus_tag="VFH91_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6861482.1"
                     /translation="MGQLDSHSYDFGRFRLKPAERVLLREGEPVPLTPKVFDILITLV
                     EKSGQVVEKDDLMKRVWPTTFVEEGNLTQNVSLLRKALGEAPGGTQFIETVPRRGYRF
                     VAEINETWADEVKPEVFKVEAIPTNGSQHVSQDSQAPIESVANTSRLAALTKGRSNVV
                     LFAATVIVLVGGVIFFAGRGKAGNGSAIQSIAVLPFVDEAGDPDSEYLNDKIAESLIN
                     SLSKLPQLRVVPRSVVAGYKGKDIDPRKIGAELNVGAVVTGRMRRHGDLISIQADLID
                     LNNVAQLWGQHYDHKLSDVLLVQDDISRDIFENLRLKLNVEEKKQLEAYGLYLKGRNA
                     WNKRTGDALLQAIDFFQQAIAIDPNYGSAYAGLADCYNMLVVYGRLQPKDGFPKAKDA
                     ATKALEIDESLAEAHTSMAFIKFRWDWDRTATEREFQTAIRLKPTYAPAHQWYSSYLV
                     AVERFDEAIDEAKRTEELEPLSFVGSSHLGWIYYLSGQNDNAIKQCKKILELDPSSFP
                     ARRYLGLAYEAKGMYAEAINEFQTGVKLSGSPLMLALLGHAYAASGKRAEALQVLADL
                     QQLPEERYVSPYTVAAIYAGLGDNDSAFKWLDKAVEERDIWLMNLKVDPVFAKLRPNS
                     RFSELLARIRLRP"
     gene            complement(3212..>4118)
                     /locus_tag="VFH91_00505"
     CDS             complement(3212..>4118)
                     /locus_tag="VFH91_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6861483.1"
                     /translation="LGGVVYGLIESPRLGFSHPLVVATLLGGAVALLIFLWNEAHSRN
                     PMVPLGLFRSRNFAGANLITLLLYAALGSLFFFLALNLIQVQRYSATAAGASLLPFIL
                     IMFVLSRWSGGLVDRFGARLPLVIGPFIAAGGFVLFALPGVGARYFSSFLPAVLVLGF
                     GMAVTVAPLTTTVMNSVHQEQVGVASGINNAVSRTAGLLAIAVFGVVMLVWFSGALNR
                     RLDQFGVDEGVKQSLLEQRVKLGGIELPAGLDENSKETITAAIDGAFVEGFRVLMLIS
                     AALAAASGLIAWAVVRSHSRATGQH"
BASE COUNT         1049 a         1100 c          992 g          977 t
ORIGIN      
        1 ggtggacgtg ccttgatatc ttccaggttg aatggaatct ccaggtcggt ccggatacgt
       61 gcaagttcac gagagcgacg gattaattcg gcgttatcgc gcagcgactc gcgatatgtc
      121 ttatgtttta ctgcttccca tccgtttaac gcagcctcga tcgacccaaa ctgctgaatt
      181 atttgaacag cgcctttcga accgataccc ggcgcgcccg ggatgttgtc aatcgaatcg
      241 cccatcaggc caagcagatc gacgagcttg tctgcggtaa ctccaaattt cttttcaacc
      301 caggcttcgt cgcaccactc aataggcggc acgggttctt tgcgctttac cacttgcgaa
      361 ttctgacgca tgcaaatgat atatggatcc tttaccagtt ggcacatgtc cttgtcattc
      421 gacacgatca ccgtttgaag ttcttgctcc gcgatcctat gggcgagagc gccgatgacg
      481 tcatctgctt cgaagcccgg cgagttgatt accgggatat tgaaggcatc acagagtcga
      541 aatatataag ggagttgaga agagagatcg cctggcatct cttcgcggtt agctttgtaa
      601 tcagcgaacg tttcgtgacg aaaagtcttt tcccgtgatt caaaaattgc agcgatatag
      661 tcaggctgct cttctcggag cagcttgatg agcatgttgg taaagatata gacggcctgc
      721 gtgacctgac cctgagctgt agcgagcggc gctgcgcgat tctgcattgg cgcgtaaaac
      781 gcacgaaaga tgtgcgacat cgagtcgatg agaaacacac gttcaggacg ttcagttttc
      841 ttcgcagctt gtttggcagg catgtgaatg gatcatagga acgccgcatg agggtgtcaa
      901 acatgcctga tcttctgtca gggtcggagg cgaatgcggg cgagtagttc tgagaaccta
      961 ctgtttgggc gcaactttgc aaataccgga tccactttca aattcatcaa ccaaatgtca
     1021 cgttcttcaa cggctttatc gagccattta aaagccgagt cattatcacc aagtcctgcg
     1081 tagatcgctg caactgtgta aggagacacg tagcgctcct cgggcagttg ttgtagatcg
     1141 gccaatacct gtagagcctc tgctcttttg cctgacgctg cgtaagcatg gcccaacaag
     1201 gccaacatca atggactccc cgagagtttc acgcccgttt gaaactcatt gatggcttcc
     1261 gcgtacattc ctttggcctc gtaggccaga ccaaggtaac gacgcgcagg aaatgagctg
     1321 ggatctagct caagaatttt cttacactgt ttgatagcgt tgtcgttctg tcccgaaagg
     1381 taatagatcc agccgagatg cgagctgccg acgaacgaca atggctcaag ctcttccgta
     1441 cgtttagcct catcaatcgc ttcgtcaaat ctctccaccg caactaagta agagctgtac
     1501 cactggtgcg cgggagcata agtcggctta agcctgatag cggtctggaa ctctctttct
     1561 gtagcagttc gatcccagtc ccagcgaaac ttaataaagg ccatcgacgt gtgtgcttcg
     1621 gcgagacttt catcaatttc aagggccttt gtcgccgcat ctttggcttt ggggaatcca
     1681 tctttgggtt gaagccgtcc gtaaacgacc agcatgttgt aacagtcggc caaacctgcg
     1741 taagcagaac cataattggg atcgatggca atagcctgtt ggaaaaagtc aatcgcctgt
     1801 agcagcgcgt cacccgtacg tttgttccat gcattcctgc ctttcaagta caaaccatag
     1861 gcttccaatt gcttcttttc ttcaacattc aatttgagtc gcagattttc gaatatatcg
     1921 cgagagatat cgtcctggac caatagcaca tcagaaagct tgtggtcgta atgctgtccc
     1981 caaagttggg ccacattatt taggtcgatc aagtcggcct gaatactgat gagatcgcca
     2041 tgacgtcgca tccttccagt aactactgct cccacattca gttctgcacc gatttttctg
     2101 ggatcgatgt ccttgccttt gtagcccgcg acaacactgc gaggaacgac tcgcagctga
     2161 ggcaatttcg aaaggctgtt gatcaagctc tcagcaatct tgtcattcag atattcggaa
     2221 tctggatcgc ccgcctcatc gacgaacgga aggaccgcga tagattgaat ggcactgccg
     2281 ttccccgctt tgccacgtcc agcaaaaaag attacgccgc caactaaaac aatcacggtt
     2341 gcggcgaaca gaactacatt agagcgtcct ttcgttaagg ctgccaatct gctggtattg
     2401 gcaactgact cgattggtgc ttgcgaatcc tgactgacat gctgactgcc gttagttggg
     2461 atagcctcaa ccttgaagac ctccggcttg acctcatccg cccaggtctc gttgatctcg
     2521 gcgacaaacc tgtaaccacg acgcgggaca gtttcaataa attgagtgcc gcccggagct
     2581 tctcccagtg cctttcgcag caaagatacg ttttgggtga gattcccttc ctcaacgaag
     2641 gtggtcggcc agacccgctt catcaagtcg tctttttcaa ccacctgtcc gcttttttcg
     2701 acaagtgtga tcaagatgtc aaagactttt ggcgttagag gtacaggttc gccttcacgc
     2761 agcagtacac gttccgctgg tttgagccga aatcgtccaa aatcataact gtgactgtct
     2821 aattgcccca taaagaacgc cttgagaaat atctttgaga atttcttgag tcgattctga
     2881 ggacttcctg ctgcccatta attaaaaatg gactcacctt caagaaatac aaagcttgat
     2941 tgaactgtcg ctacagattg cgtaatggga ttgtaatgca agcaccagac gtgggcaacg
     3001 gcttcaaaaa aacgtttcgc ttccaccccg cgcgatgtag ggcggagcgc tccagcctca
     3061 gcggcagagg ctggacgcaa cgttgatatc aagaatagaa ggtctcccaa tgattgagcc
     3121 tgaaagaact tcggaagtag tcgagacttc gcttatttgt gcgctaagcg tcgcggttgc
     3181 tgcgctaggc ttttttctcg gttggctgat ctcaatgctg accggttgcg cgcgagtgtg
     3241 aacgaaccac cgcccacgca attaagccac tcgcagctgc taaggcagct gaaatcagca
     3301 ttaacacgcg aaatccttcg acaaatgctc catcgatagc ggcggtgata gtttctttac
     3361 tattctcgtc caaacctgca ggtagctcga taccaccgag tttcactcgc tgctccagaa
     3421 gactttgctt tacaccttca tcaacgccga actgatctaa ccgccggtta agagctccgc
     3481 tgaaccaaac cagcatcaca acaccaaaga ccgcgatcgc cagcaaacct gcggtccgcg
     3541 acacagcatt attaataccc gacgcgaccc ccacctgctc ctgatgcact gagttcatca
     3601 cggtagtagt caacggagct actgttacag ccataccgaa tcccagcacc aaaaccgcgg
     3661 gaagaaagct ggaaaagtac cgagccccaa cgcccggaag ggcaaacaag acaaatcctc
     3721 cagcagctat gaatggtcca attacgagtg gtaaccgcgc accgaagcgg tcaaccaacc
     3781 cgcccgacca acgagaaagc acaaacatga tcaaaataaa cggaagcagt gaagcgccgg
     3841 cagcggtagc cgaatacctt tgaacctgaa tgagattgag cgccaggaag aagaaaagcg
     3901 atcccagagc ggcgtataaa agtagagtta ttagatttgc tcccgcgaaa ttacgcgaac
     3961 ggaacagccc aagcggcacc atcggattcc tggaatgcgc ctcgttccac agaaaaatca
     4021 ggagggccac agcgcccccc agtagagttg caaccactaa gggatgtgag aatcctaaac
     4081 gcggcgactc aatcaaccca taaaccactc caccaagc
//
LOCUS       DASSFP010000023         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_5381, whole genome shotgun sequence.
ACCESSION   DASSFP010000023 DASSFP010000000
VERSION     DASSFP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5381"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFH91_00510"
     CDS             <1..692
                     /locus_tag="VFH91_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6861484.1"
                     /translation="VKVQLSYCTIRSPIDGRAGQRLVDIGNVVNPGGSSNNGSASSSN
                     SANTNALLIIERIDPIYADFTISQNNLSQVQQQMRANRLTAEVRLPDDPANPVIGQLT
                     FLNNSVEDTTGTVNLRATVPNSDHRFWPGRFVNVRLVLNTLHQAVLIPSTAPQMAANG
                     SFVYVVKQDSTAEQRPVTLGQRQGDLIVVESGVTAGEHVVTAGQLGVTPGGKVKVEAP
                     AATNAATTQAK"
     gene            692..3856
                     /locus_tag="VFH91_00515"
     CDS             692..3856
                     /locus_tag="VFH91_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6861485.1"
                     /translation="MNLSEPFIKRPVMTAVLTLSVVLFGILSYQRLPVNDLPQVDYPV
                     IQVQVGYPGASPDTIANNIATPLERQFMQINGLEVVTSSSKQGVATFTLQFALEKNID
                     AAATDVQTAISQAISSLPADLPSPPTYSKTNPNDQPIMYIALTSDSVTPGQLYDYAST
                     QVGQRMSILPGVSQVNVYGTKSAIRIKANPSAMWARGISIDDLNNAIKNGTSYTGAGQ
                     LDSSSGTAILRPQGQLETAEQYSNLIIGGTGNAPVYLRDVAEVKESVQDERINMRFWV
                     RGIQVPSATVIVAVNRRAGANAVEVASSIRQMLPQLSTELPGSIRITPIYDRSLSIVH
                     SVADVQMTLIIAFVLVVIVIFAFLGRATDTLIPTVALPLSLLITFMAMHALGYSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRMEHGEQPFEAAINSAKEISFTIMSMTISLAAV
                     FIPLVFMSGLVGRIFREFAVTIVIAIFASGLVSLTLTPLMCARLLKARGEGSQRTWME
                     RVIGGIEKRVLYLYGGSLWWFLKHRWISPIIWVICLAGTIWLFILVPKAFLPPGDSSV
                     IFGAFIAREGSSPAQMRELQNRVDETLHQDENVITDFTLTGATGFLASNQGITFTFIR
                     PPEERKPIPEVTAELMGRLNSIPGVFSFLRPFPVLEISTGVTSQQQGQYAFAVSGANP
                     SEVYDVSQKLMAKLMTYPGFLTVSSDYYHNTPNVNIDLRRDQAKTYGVSEARILSLLR
                     NAYSQNYLYLIKKPEDQYQVILEIADEARSNPEDLAQLYIRSDDGQRLVPLGELVSWK
                     TTLGPQTVNHLNQFTSVTLFFNLKPGVALGDATNFITKSAAETVPPGVRGELVGEAQT
                     FSSTVTSLTFLMALAVFVMYVILAILYESYVHPLTVLSTLPTALVGGLLTLLLFRQEA
                     SLYAFVGMFMLMGIVKKNGIMIVDFARQRVEAGETAEQAIHDASMDRFRPILMTTLAA
                     VFGALPIALGFGADGSSRRPLGLVVVGGLIISQFITLYITPVIYLYLERFQENVLDRS
                     AFFGSRKTRKEPPTHTPSDVPAGD"
BASE COUNT         1062 a         1170 c         1142 g         1109 t
ORIGIN      
        1 cggtcaaggt ccaactaagt tactgcacaa ttcgatcgcc aattgacgga cgcgccggtc
       61 agcgacttgt cgatattggt aacgtagtca atccgggtgg cagttctaac aacggcagcg
      121 ccagcagtag taattctgcg aacaccaatg cgttactgat catcgaacga attgatccga
      181 tctacgccga tttcaccatc tcgcagaata acctgtccca ggtccaacag cagatgcgag
      241 cgaatcgatt aacagctgag gttcgtctcc cggacgatcc ggcaaatcca gtgatcggac
      301 aactcacgtt tttaaacaat tccgttgagg atacaaccgg aacggtcaat ctcagggcaa
      361 cagttccaaa ctcggaccat cgtttctggc caggacgttt tgtaaatgtg cgtctggtcc
      421 taaacaccct acaccaggcg gttctcattc cctccactgc gccgcagatg gcagctaatg
      481 gatcttttgt gtacgtcgtc aaacaagact cgactgcaga acaacgaccc gtaacgctgg
      541 gtcaacgtca gggcgactta attgtagtgg aaagtggtgt gacagccggc gaacatgtcg
      601 tcacggcggg tcagttgggt gtgacgccag gcggaaaggt taaagtcgag gcacccgcag
      661 caacgaacgc tgcgacaacg caagcgaagt aatgaactta tctgaaccat tcatcaagcg
      721 accggtcatg acagctgtct tgactctctc agtagtcctc tttggaatat tgagctatca
      781 acgactgcct gttaacgatc tgcctcaagt cgattatcca gttatccagg ttcaggtggg
      841 atatccgggt gcgagtcccg acacgatcgc taataacatt gccacgccac tcgagcggca
      901 gtttatgcag atcaacgggc tcgaagtggt cacgtcctca agtaagcagg gtgtggcaac
      961 tttcacgttg cagtttgctc ttgagaaaaa catcgatgcg gcagcgactg acgtacagac
     1021 cgcaatctcg caagccatca gcagtcttcc cgctgactta ccatcgccac ctacatactc
     1081 caagacgaat ccaaatgatc aaccgatcat gtatatcgct ctcaccagcg attccgtaac
     1141 gccgggacaa ctctacgact atgcgagtac ccaggtgggc caacgcatga gcatcctgcc
     1201 gggtgtttcg caggtcaacg tctatggaac caaatcagct attcgaatta aagcgaaccc
     1261 atcagccatg tgggcgcgcg gaatctcgat tgatgatcta aataacgcta tcaagaacgg
     1321 aacgagctat accggcgcag gccaactcga cagtagttca ggcactgcta ttctgagacc
     1381 acaaggtcaa ctcgagacgg cagagcaata cagcaatttg ataatcggtg ggactggcaa
     1441 cgctccggtt tatctacgcg acgtcgcgga agtcaaagag tcggtccagg acgaacgcat
     1501 caacatgcgt ttttgggtcc gtggcattca agtcccctcc gcgacggtaa ttgttgcagt
     1561 taacaggcgt gctggagcca acgcggtcga ggtggcaagc agcatcaggc agatgctacc
     1621 gcagctcagt accgaacttc ccggatcgat ccgcatcacc ccaatttatg accgatcgtt
     1681 gagcatcgtt cattccgtag cggatgttca gatgaccctc atcatcgcgt ttgtgcttgt
     1741 cgtcatcgtc atctttgcgt ttctgggccg ggccactgac accctcattc cgaccgttgc
     1801 actgccgttg tctttactca ttaccttcat ggcaatgcac gcgcttgggt acagcctcga
     1861 taatttgtcg ttgatggcct taacgctcgc gattggattt ctcgttgatg atgcgatcgt
     1921 attcctggag aacaccgttc gcagaatgga gcacggagag caaccattcg aagcggcaat
     1981 caatagcgca aaagagatca gcttcacgat catgtcgatg acgatctctc ttgcggcggt
     2041 ctttattccg ctcgtattca tgagtggtct cgtgggtcga atttttcgtg agttcgcagt
     2101 cacgatcgtt attgctatct tcgcttcggg tctggtctcc ctcacactga ctccactgat
     2161 gtgtgcgcgt ttattgaagg ctcgcggtga aggttcccag cgtacatgga tggagcgggt
     2221 catcggtgga atcgaaaagc gagtgctcta tttgtatggt ggatcgctct ggtggttttt
     2281 gaaacatcgg tggatctcgc caatcatctg ggtgatctgt ttggcgggca caatctggct
     2341 tttcattctg gtcccaaaag ctttcctacc tccgggtgat agcagtgtga tttttggtgc
     2401 tttcatcgcc cgcgaaggtt cgtcgccggc acagatgaga gaactacaaa accgcgtcga
     2461 cgaaactctt catcaggacg agaatgtcat caccgacttt acgttgactg gagccaccgg
     2521 gtttctcgcg tccaaccagg gcatcacgtt cacgtttatt agaccaccag aagaacgtaa
     2581 gccaatcccg gaagttaccg cagagttgat gggcagactc aactcgatcc ctggagtttt
     2641 ttcatttctg agaccgtttc cagttttaga gatcagcacg ggggtaacaa gtcaacaaca
     2701 gggacagtat gcgttcgcgg tttcgggtgc gaatccaagt gaagtctatg acgtaagcca
     2761 gaaattgatg gccaagctga tgacctatcc tggatttttg actgtttctt ccgactacta
     2821 tcacaacaca ccgaacgtca atattgattt acgcagagat caggcgaaaa cttatggcgt
     2881 ttcagaagcc cgcattctct cgttactgcg caatgcctac tcacaaaact atctttacct
     2941 gattaaaaaa cctgaggacc agtatcaggt catactggaa attgcggacg aggctcgctc
     3001 taatcccgaa gacctggccc agctttacat caggtccgat gatggtcagc gacttgtgcc
     3061 acttggtgaa ctggtatcct ggaaaaccac tcttggtcca caaacagtca accatttgaa
     3121 ccagttcacc agcgtgactt tatttttcaa tctaaagcca ggcgtggcgc tgggtgatgc
     3181 gactaatttc ataactaaat cagcagccga aactgtgcct cccggagtgc gaggggagct
     3241 tgtcggagaa gcgcaaacct tttcgagcac cgttacgagc ctgacgtttc tgatggcgct
     3301 cgcagtgttt gttatgtacg tgattctcgc catcctgtat gagagttacg tccatcctct
     3361 aacagtgctt tctacgctac ctacggccct ggtcggaggg ttgttgacgc tgctgctatt
     3421 tcgtcaggaa gcgtcgcttt acgcgttcgt cggaatgttt atgttgatgg gtatcgtgaa
     3481 gaaaaatgga atcatgatcg tcgacttcgc gcgacagcgc gtggaggcag gcgaaaccgc
     3541 tgagcaagca attcacgatg cgagcatgga ccgcttcagg ccaatactga tgacgaccct
     3601 ggcagcagtt ttcggcgcgc tgccaatcgc tctgggattt ggtgctgatg gttcttcacg
     3661 gcgcccactt ggtctggtcg tggtgggtgg cttgatcatc tctcaattca taacgctgta
     3721 cattactccg gtgatctatc tgtacctcga gcgttttcag gagaatgttc tggaccgcag
     3781 cgcatttttc ggctcacgca agactcgcaa ggaacctcct actcataccc cgtccgacgt
     3841 ccccgccggc gactgagccg agtccctctc aaagtgagcg tatcgccaga gggctttgcc
     3901 tcaaaagtct gaaatctcaa ctctcattca caccgggctt tcagcccggt gatcatgcgc
     3961 taagagatta gtggaaccgt tttaaacggt ttgctcgtct tctcaacgcc gcgaggcggt
     4021 tgagaacgaa gcggttagtt catgagccgg ccggagaaac cgttgaaacg gttccttgag
     4081 cttgtcgtag cgcctgatca ccgggctgaa gcccggtgtg aatgagagag atttcagact
     4141 tttgaggcaa agcctcgcca cagagaattt agtagaatat tccctcgcca ttcgagaatt
     4201 gtaaatatca cgggttgttt ggagtcgagt tatgcgtgtg actgatgaaa tctcaaaaag
     4261 tgatgatatg gtggattctc gttggacgat gactgtagct tcatggcttc tcgtagagcc
     4321 ggcagcggtg aagtttttcc tctcgcactc gctttaactg gacatggctg aactatcgcc
     4381 gaacacacaa gcaatcttgc tcttgacggc tcctctaatc actggcgccg acgacaattc
     4441 gtccgaactg tttacgcccg ctgagtatcg caagctcgag tct
//
LOCUS       DASSFP010000024         6816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1941133, whole genome shotgun sequence.
ACCESSION   DASSFP010000024 DASSFP010000000
VERSION     DASSFP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941133"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..2332
                     /gene="thrS"
                     /locus_tag="VFH91_00520"
     CDS             341..2332
                     /gene="thrS"
                     /locus_tag="VFH91_00520"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET6861486.1"
                     /translation="MSDINVQLKDGAPVVLPNSATVAEALKKLDRDVAKQALAAKVGG
                     HEVDLAAPLSTLPSENGESENSVVQIEPIVPQTRDGLEVLRHSTAHLLAAAVLDLFPG
                     TKLGIGPALLDDPRYGYFYDVIAPRPLTEADLPAIEKRMKQIASQNLAYRREEISKAE
                     ALRVFGEREEPLKCELIDEKAGERVSVYYIDGSPFIDFCLGPHVPNTNKLRAFKLLSL
                     AGAYWKGDAAQPQMQRIYGTAFFSQEELDGWLKQREEAEKRDHRRLGRELDLFSIQED
                     YGQGLIFWHPKGGAIRKEMEDYLREELLKREYGLVFTPHIAKRDLWFTSGHEENYVDS
                     MFSPMEFEEQEFRIKPMNCPFHIGIYKSAQRSYRELPLRYAELGTVYRAELSGTLHGL
                     TRVRGFTQDDAHIFCTPETIGQEILGCIDFAYNLFQVFGFKDIKVELSVRGNDPDAHY
                     LGSDEDWESAERNLVDALNSRDIPYERIEGEAVFYGPKIDIRVEDAIGRNWQLTTVQF
                     DFNLPRRFQLEYIGEDNKPHQPLMVHRALFGSMERFFGVLIEHYAGAFPMWLAPVQVA
                     VLPITDRINGYAEEITKELRSLGMRVESNLRSEKIGAKIRNAQLMKVPFMLVLGDREM
                     EQKMVAVRERTKGDIGVMSLAEFETMARKLITTRALTNQ"
     gene            2386..3027
                     /gene="infC"
                     /locus_tag="VFH91_00525"
     CDS             2386..3027
                     /gene="infC"
                     /locus_tag="VFH91_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET6861487.1"
                     /translation="MDLKTQTVGGAAIATGFRGRGQRPDNRRQPPVRVNERIRVPEVR
                     VIGEEGEQLGVMDVRDAIRSAREKGLDLVEVAPNADPPVCRIIDFGKYQYEAKKKANE
                     AKKKQVTITVKEVKFRPGTDDHDYEYRMKHAREWLQGGDKVKATIWFRGREMTHRELG
                     ARILEKLEQDLADVGEVEARPRMEGNQMFIVLGPKRHKGSAKPDQHTSVPPGQ"
     gene            3121..3324
                     /gene="rpmI"
                     /locus_tag="VFH91_00530"
     CDS             3121..3324
                     /gene="rpmI"
                     /locus_tag="VFH91_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET6861488.1"
                     /translation="MPKLKTHKGAAKRFRLTATGKIKRGHSHARHILTKKTNKRKRQL
                     DIDVLVSKADHDRVTSMLPYGRD"
     gene            3391..3774
                     /gene="rplT"
                     /locus_tag="VFH91_00535"
     CDS             3391..3774
                     /gene="rplT"
                     /locus_tag="VFH91_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET6861489.1"
                     /translation="MPRAKRGNKRLERRKKILKLAKGYRGTKSKLYRSAKESVERGLN
                     FAYTGRKLKKRDFRSLWIVRIGAAARLNGLNYSQLMHGLKLAAIELDRKVLADLAVHQ
                     PDSFGKVVEQAKAALDNEQRKKAAA"
     gene            3746..4504
                     /locus_tag="VFH91_00540"
     CDS             3746..4504
                     /locus_tag="VFH91_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861490.1"
                     /translation="MNSAKRLQLRSQGLGKDITMRQFRNYSQYISRFAAASLVVLLSS
                     FAAFSQTAPTSVAGKYEGVAKVAGSADQKLTLDIKSEGSKISGQLISGQLTSDISEGT
                     IVGDKLTLKLGTAGKDGVITAKMDGEKLSGDWIAGTQKKVVELTKAVTPSVAETVNLS
                     GQWEGVADAQGQPFPFLLVLKIDGENVTGSSSSQLGESTIKDGTWKDGKLNFQLDSPS
                     GVIVMSAVVVEGKLSGEFDYSGQASGKWVAVKKN"
     gene            4506..5609
                     /locus_tag="VFH91_00545"
     CDS             4506..5609
                     /locus_tag="VFH91_00545"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET6861491.1"
                     /translation="MSGTENELNPTEQVKTARKAFEQEFERFARFTESSTASNMSLEQ
                     ALEEQRSLAELRTRHLGKKSELAATRKLIGRVAADDRASFGQLVQTTEAFITDQLDRV
                     EQSLKESIEASRTALESIDVTLPGRRPRRGHAHPITLVRERIEDIFVALGYVIEDGRE
                     IETDFYNFDALNIPENHPARASQDTFYTTEGFALRSQTSTVQIHAMQRRGAPVRMIAP
                     GRVFRRDTPDPTHNPMFYQVEGLCVDRGITMAHLKGTVAEFLRRMFGPDTVTRFRPSY
                     FPFTEPSAEFDFSCFKCKGSGCRICKNSGWIELGGSGMVHPNVLRAVGVDPQIYSGFA
                     FGLGIDRMCALMYGLDDIRLLFENDVRFLEQFS"
     gene            5657..>6816
                     /locus_tag="VFH91_00550"
     CDS             5657..>6816
                     /locus_tag="VFH91_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HET6861492.1"
                     /translation="MLISYQWLRELSATVLSPAELRDRLTMVGLAIDAVETKETDSVL
                     DVEVPSNRPDCLSHVGIAREVTVIEKSHLRLPVAKALKTDGSAAELTAVTIDDPDLCP
                     RYAARLIRGVKIGPSPEWLVQQLEVIGQRSINNVADITNYVLHEVGQPLHAFDFNKLS
                     GKRIVVRRAAAGEKLKTLDGVERELTTDTLVIADAERPVALAGIMGGEDSEISQTTTD
                     VLIESAYFDPHSIRRTARRLGMDTEASRRFERGADRENVVNAQNRCIELICELAGGVP
                     TKDLIDVYPQKLQPRSVTLRIHRVKDLTSLSVEPAEMDRILTALGFVKQSETQDELVY
                     LVPSWRNDVEQEEDLIEEIARHTGFEKIASELPPSSSSGEYQPGEMKQRILRR"
BASE COUNT         1742 a         1600 c         1894 g         1580 t
ORIGIN      
        1 ggtgcttgct atcatccggc ttcgttgttt gcttaaaggc aggaaattgc tgccatttct
       61 aacttttgat aggacgctcc agaagacctt cgttatgaat cgaacataca ctcgattctt
      121 tactttttaa gaccgacccg ccaccacgcg ggcgcgcgac gaaggcgctc ctatcgaatc
      181 cgatcgactg aagttagtaa atagtgaatc gaaaatcgtt ggagttggtc ttcggcctcg
      241 ggataaaggt cttttttgac tgtccgtggc gctcctaaac ggagctgagg gccaaatagt
      301 gtcaaccggg ttagccagtt gattctgttg gcgtttttaa atgagtgaca taaacgtaca
      361 actaaaagac ggagcccccg tagttttgcc aaactcagct acggtcgctg aggctctaaa
      421 gaagctcgat cgcgacgttg cgaagcaggc tctcgccgca aaggtgggag gtcacgaggt
      481 cgacctggct gcgcccctga gcacacttcc ttcagaaaac ggcgaatccg aaaattcggt
      541 ggttcagatt gaaccaatcg ttccccaaac tcgagacgga ctcgaagtcc ttcgtcattc
      601 gactgctcat ctattggcgg ccgcggttct ggatttgttt ccgggtacga agcttgggat
      661 tggaccagcc ttacttgacg atccccgata cggctacttc tacgatgtca tcgctccgcg
      721 tcctttgacc gaagccgatt taccggcgat tgagaaaagg atgaagcaaa ttgccagtca
      781 gaatctcgcg tatcgccgtg aagaaatttc caaagcagaa gcgctgagag tgtttggcga
      841 gcgcgaggag cctctgaagt gtgagctgat cgacgagaaa gccggcgaac gagtcagtgt
      901 ctattacatc gatggctcgc cattcatcga tttttgtctt ggtccacacg ttccaaatac
      961 caacaagctg cgagctttca aattgttgtc gctggccggt gcttactgga agggcgacgc
     1021 ggcgcagccg caaatgcaga ggatctatgg cactgcgttc ttttctcagg aagaacttga
     1081 tggctggctg aaacaacgag aagaagctga aaaacgtgac catcgacggc ttggacgcga
     1141 gctcgatctg ttttcaatac aggaagatta cgggcagggc ttgatcttct ggcatccgaa
     1201 gggcggcgcc attcggaagg agatggaaga ttatctgcgc gaagagcttt tgaagcgcga
     1261 gtacggtctc gtctttacgc cacatattgc caagcgtgat ctttggttta cgagcggcca
     1321 cgaagagaac tacgtagatt cgatgttttc gccgatggag tttgaggagc aagagttccg
     1381 cattaagccg atgaactgtc cgttccatat cggaatctac aagtcggccc aacgctctta
     1441 tcgtgaattg ccgcttcgct acgctgagtt ggggaccgtt tatcgtgcag aactgtccgg
     1501 cactttgcat ggtctaacgc gcgtgcgagg cttcacgcag gatgatgctc atattttctg
     1561 tacgcccgaa acaatcggac aagagattct ggggtgcatc gacttcgcct ataacctttt
     1621 ccaggttttt ggtttcaagg atattaaggt cgaactgtcc gtgcgtggca atgatcccga
     1681 tgcccattac ctcggttccg atgaagattg ggaaagtgcc gaacgcaacc tcgtcgatgc
     1741 tttaaatagt cgtgatattc cctacgagcg aatcgagggt gaagctgttt tctacggacc
     1801 gaagatcgat attcgagtgg aagacgcgat cggccgcaac tggcagctta cgactgtcca
     1861 gtttgatttc aacctgccgc gacgatttca attggagtat atcggcgaag ataacaagcc
     1921 acatcaaccg ttgatggttc atcgagcctt gttcggttcg atggaaagat tttttggggt
     1981 acttatcgag cattatgccg gagccttccc gatgtggttg gcgcccgtgc aggtggcggt
     2041 attgccgatt acagatcgga tcaacggtta cgcggaagaa attacaaagg agcttcgtag
     2101 tctgggcatg cgagtagagt ctaatctccg cagcgagaag atcggagcga agatccgaaa
     2161 tgcacagctg atgaaagtac cgtttatgct tgttcttgga gatcgggaaa tggagcagaa
     2221 gatggtggct gtgcgtgaac gaacaaaggg cgacatcggg gtaatgtcgt tggctgaatt
     2281 tgaaacaatg gcgcgcaagc taatcacgac gcgtgcctta accaatcaat agtcgagggt
     2341 ccatccgtcc ctgcggccaa cgactccgac tttggacgtt ggaagttgga cctgaagact
     2401 caaacagtag gaggtgcagc aatagctaca ggctttcgcg ggcgcggtca gcgtcctgat
     2461 aatcggcgtc aaccgccggt tcgtgttaac gaaagaattc gcgtacctga agtgcgcgtc
     2521 attggagaag aaggcgaaca actcggcgtg atggacgtcc gagatgcaat tcgctcagca
     2581 agagaaaaag gcttggacct ggtggaggtg gcccccaatg ccgatcctcc ggtatgtcgc
     2641 atcatcgact tcggaaagta tcagtacgaa gccaagaaga aagcgaacga ggcgaaaaag
     2701 aaacaggtaa ccatcacggt caaggaagtt aagtttcgcc ccggcacgga tgaccacgat
     2761 tacgaatatc gcatgaagca tgcgcgtgag tggttacagg gtggcgacaa ggtcaaagcg
     2821 acgatctggt ttcgtggacg agaaatgact caccgggaat tgggtgcgcg aatcctcgaa
     2881 aaactggaac aggatcttgc tgatgtgggt gaagttgaag ctcgtcccag gatggaagga
     2941 aatcagatgt tcatcgtgct gggaccaaaa cgccataagg gcagtgctaa gcctgatcag
     3001 catactagcg tgcccccagg tcagtagaag tgtcagtaag aattgtcagt aagaagtttc
     3061 cacgccttct ggattagcta cggcagttgg aaatcgaatt cgtaatagag agggttaact
     3121 atgcctaaat tgaagacaca taagggagct gctaagcgat tcaggcttac tgcgactgga
     3181 aagatcaagc gagggcactc gcatgctcgt catattctga cgaagaaaac aaataagcga
     3241 aagcgacagc tggatattga cgtgctggtc tcaaaagcag atcacgatcg agttacttcc
     3301 atgctgccgt atggaagaga ctaataccaa tattgatttg tgacgagtga aggcgattca
     3361 ttcgatcgtt tttaaagaaa ggggaccaac atgcctcgag caaaacgtgg aaataagcgt
     3421 ctcgagagac gcaagaagat attaaagtta gccaagggct atcgtggcac taaatccaag
     3481 ctatataggt cagcaaaaga gtcggtcgag cgcggtttga atttcgctta taccggccgc
     3541 aaattgaaaa aacgtgattt tagaagtttg tggattgtca gaattggtgc ggccgcgcgt
     3601 ctcaatggcc tcaactattc ccaactcatg catggcctta aactggctgc aatagagttg
     3661 gaccgcaaag tactcgccga cctggcggtt catcaaccag actcatttgg aaaagttgtc
     3721 gagcaggcaa aggcggcgtt ggacaatgaa cagcgcaaaa aggctgcagc ttaggtcgca
     3781 gggcttggga aaggatatta cgatgcgtca gtttcgtaac tacagccagt acataagtcg
     3841 atttgctgct gcctcgttgg tggtcttgct ttcgagtttt gccgcgtttt cacaaacggc
     3901 tcccacttca gtggccggaa aatatgaagg cgtggcgaaa gttgcaggtt cggccgatca
     3961 gaaattgact ttggatatca agagcgaagg ctcaaagata tccggtcaat tgattagcgg
     4021 tcagctgact agcgatatct ccgagggaac catcgttggc gacaaattaa cacttaaact
     4081 tggcactgcc ggaaaagacg gagtgataac cgcgaagatg gatggtgaaa agttaagtgg
     4141 tgattggatc gccggaaccc aaaagaaagt tgtcgagctt acaaaagctg tcacaccaag
     4201 tgtcgccgaa accgttaacc tgagtggaca atgggaaggc gttgccgacg ctcagggtca
     4261 accgtttcct tttctactgg tcctgaaaat agatggggaa aatgttactg gcagcagtag
     4321 ttctcagcta ggcgagtcga ccatcaaaga tgggacatgg aaagatggga aactcaattt
     4381 tcaactcgat tctccaagcg gcgtaatcgt gatgtcagcc gtcgtggttg aagggaagct
     4441 gtcaggagag ttcgattatt cgggtcaagc ttctggaaaa tgggtagcgg ttaaaaagaa
     4501 ctgagatgtc gggaaccgaa aacgagctca acccaactga gcaggtaaag acagcacgca
     4561 aagcgttcga gcaagagttc gaacgctttg cccgctttac cgagagttcg actgcttcaa
     4621 atatgtcgct tgagcaggct ttggaggaac agcgatcctt agcggaactg cggacgcgtc
     4681 acctcggaaa gaagagcgag ctcgccgcta caagaaagct gatagggcga gttgcggccg
     4741 atgaccgggc atcgtttggg caattagttc aaacaaccga ggcctttatt actgaccagc
     4801 tagatcgggt cgaacagagt ctgaaggaat cgattgaagc cagtagaaca gcgcttgaga
     4861 gcatcgatgt aaccctgccg ggccggcgtc cccgtcgcgg acatgcgcat ccgattaccc
     4921 ttgtaagaga gcgcatcgaa gacattttcg tcgcactcgg ttacgtcatc gaagacggtc
     4981 gcgagatcga gaccgatttc tacaacttcg atgccctcaa catacccgag aatcatccgg
     5041 cgcgtgcgtc gcaagacacg ttctatacga ccgagggctt cgcacttcgg tcacagactt
     5101 cgacggtaca gattcatgca atgcagcgtc gaggcgcacc cgtacggatg atcgctcccg
     5161 gccgagtgtt tcgtcgtgat accccagatc ctactcacaa tccaatgttc tatcaggtcg
     5221 agggactttg tgttgatcgc ggcatcacaa tggctcatct taaaggcacg gtcgccgagt
     5281 tcctgcgcag aatgttcggg ccggatactg ttacgcgatt ccgaccatcc tatttcccgt
     5341 tcaccgaacc cagcgccgag ttcgacttca gctgtttcaa atgcaagggc tctggctgtc
     5401 ggatttgtaa aaactccggt tggattgaac ttggtggttc agggatggtg caccctaacg
     5461 tgctccgtgc cgtcggggtc gatccgcaga tttattcagg tttcgctttt ggcctgggca
     5521 ttgatcgcat gtgtgccttg atgtacggct tggacgatat tcgcctgcta ttcgagaacg
     5581 acgtcagatt cctcgagcag ttttcgtaaa gggttccgtt aaagcgttca gttaaagcgt
     5641 tcagttaaag atcgtcatgt taatcagcta ccaatggctc cgtgaattgt cggccaccgt
     5701 gctttcgccg gctgaactgc gtgaccggct taccatggtt ggcctggcaa ttgacgcagt
     5761 tgagaccaaa gagacagatt cagtcctgga cgtcgaggtc ccgtcaaatc ggcccgactg
     5821 cttgtcgcat gttgggattg cgagggaagt taccgtaatt gaaaagagtc acctgcgatt
     5881 acctgttgca aaggccctga aaacagacgg tagtgcagct gagttgactg cggtaacgat
     5941 agacgatccg gatttgtgtc cccggtatgc tgcgagactg attcgtgggg tgaagattgg
     6001 gccatcacct gagtggctgg tgcaacaact cgaagtaatc ggccaacggt ccattaacaa
     6061 tgtggcggac attaccaact acgttcttca tgaggtagga caacccctac acgcctttga
     6121 cttcaacaaa ctttccggca aacgaattgt tgttcgccgg gcggcggcgg gtgaaaaact
     6181 aaaaacgttg gatggagttg agcgtgaact gaccactgat actttagtga tagccgatgc
     6241 ggagcgcccg gtggccctcg ccggaatcat gggcggagaa gactcggaga tttctcaaac
     6301 taccaccgac gtcctgattg aaagtgcgta tttcgatccc cattcaattc gtcgcactgc
     6361 gcgccgtctg gggatggaca ctgaagcttc gcgacggttt gagcggggcg ccgatcgcga
     6421 aaacgtagtc aacgcccaaa accgatgtat cgaattaatt tgtgaactcg ccggtggcgt
     6481 gcctactaaa gatctgattg atgtctatcc gcaaaaactg cagccacgca gcgtgactct
     6541 tcggattcat agagtaaagg acctaacatc gctgtcggtc gaacctgcgg agatggatcg
     6601 aattcttacg gcgcttggct ttgtaaaaca gtcagaaact caggatgaac ttgtctatct
     6661 cgtcccttcg tggcgcaatg acgtcgagca agaagaagac ctgattgaag agatcgcccg
     6721 tcatactggc ttcgaaaaga tcgcctcgga gttgccgcct tcaagttcat cgggggaata
     6781 tcagcccggc gaaatgaagc aacggattct gcggcg
//
LOCUS       DASSFP010000025         7005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1643636, whole genome shotgun sequence.
ACCESSION   DASSFP010000025 DASSFP010000000
VERSION     DASSFP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643636"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1796)
                     /gene="ftsH"
                     /locus_tag="VFH91_00555"
     CDS             complement(6..1796)
                     /gene="ftsH"
                     /locus_tag="VFH91_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET6861493.1"
                     /translation="MPTPAPKVSLRSRRVALVCLTVFIITVILSIAAINYFRQRQQVQ
                     IINYSQLYALGQAGSAQALQIEGETVSVTKPDGSVVVATVTGEAAQHELVQIFREKNV
                     PVEFRPMQPGPLTTALNWILPIFSLALLGVVGWRVHASMSGHGSFDLQDQNGKQEVGF
                     NDVAGVDEARAELTETIEFLRNPEKFSRLGGRVPRGILLYGPPGTGKTLLARAAAQEA
                     GVPFLAASGSTFQEKFAGLGAARVRSLFAKGRKLSPCIIFIDEIDALGRRRGRGGDSA
                     SADQDQTLNQLLVEMDGFDQSSTVIVIASTNRPDILDKALTRPGRFDREIAVNLADVR
                     GREEILKVHSRKLKLEEGLDLQWIARGTPGFSGAELANLMNEAAICATRDNAEAIGRP
                     HIEHARDRVLMGAERQGFTMDEDERYATAVHEAGHVAVGLLVEHGDPIHKVSILPRGR
                     ALGVTQSLPERDRLMKKREYLEDQIALLLGGRAAEQVILSTMTAGASNDIERAVEIAR
                     RMVSEFGMSKLGPIHLGQSDQPHSQTLLDRIEEATNELISHQLERACEIVDAERDSIA
                     RLVELLMDRDTVDREEIQACFGLGKSSQAA"
     gene            complement(1848..1994)
                     /locus_tag="VFH91_00560"
     CDS             complement(1848..1994)
                     /locus_tag="VFH91_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861494.1"
                     /translation="MAVKLFTGVRLSALTQCNVVALPLLSFRVRALPKKFRRFPDPIL
                     FSSK"
     gene            2357..2509
                     /locus_tag="VFH91_00565"
     CDS             2357..2509
                     /locus_tag="VFH91_00565"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET6861495.1"
                     /translation="MLWTIFVILLVLWLLGMVSSYTIGGFIHILLVLALVVLLINIIG
                     GRRTVV"
     gene            complement(2593..4305)
                     /locus_tag="VFH91_00570"
     CDS             complement(2593..4305)
                     /locus_tag="VFH91_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET6861496.1"
                     /translation="MTNNDPSEWKTHAEQTDYRETPSYADTIDFCRRLAAASLTIEFR
                     SFGKSGQGRDLPLLVASETQTFTPEAAKSQGKAVVLIQACIHSGEPDGKDAGFALLRD
                     LAITKTVVGILENVVLLFIPIYNTDGHERSSPYNRINQNGPVSMGWRTTRTYQNLNRD
                     YMKADTPETRAWLKLWNEWQPDLFIDCHVSDGADYRSNITFHFERHEGVDPEVVSWEQ
                     DVFRNKVAAATAAAGNTISWYLEFIDNRDLPRGMRDFNGPPRFSTGYVPLRNRPGILI
                     ETHMLKDYRSRVKGTYDFLRAALSEVNKDPKRLIELGQKADKRASENSKYPLEFELTD
                     IPTPFELKAFSYETEQSEISGDLRVIYGDEPLDLTVPMYETLRVTTSVVPPLAYIVPV
                     EWSEVIEVLTTHGLRTQRLTNTASVPVESYRFVDVEWPGMPFEGRHMPRFKVLPTNET
                     REFAAGSLVVPVDQPLARLVLGLLEPQAPDSFVRWGFFNAIFEEKEYGEPYVLEQLAR
                     EMISANPELNDIFEEALQRDPQFAQNPYERLKYFYRRSPYWDPQMSLYPVGRITSVLD
                     LPAI"
     gene            complement(4583..5365)
                     /locus_tag="VFH91_00575"
     CDS             complement(4583..5365)
                     /locus_tag="VFH91_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861497.1"
                     /translation="MEGITGVFRSQEEAERTVVQLQQIGIAKKRLGLLVPGSNPHQIE
                     RAVPTVDTEEPGMGKAMGAAVGGAMGAAGGATVGLAAATLAIPGVGPVLAFGLLGAAL
                     LGVGGAVAGAKVGETLEEELGEGLPREDVYLYEDALRRGHTVVIAYAEEGSQASKARE
                     IFDRNGAEDEETLRENWWQPIRDRERESYATIGKNFDTDEMSYRRGFHAAQHPDRRGK
                     LYADVEPHLRELYADDELDTAFQHGYERGRSFYVSSMGIRRD"
     gene            5524..5895
                     /locus_tag="VFH91_00580"
     CDS             5524..5895
                     /locus_tag="VFH91_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861498.1"
                     /translation="MFDIVDKQWPMSVGDGKATRKKRPRILAITTDCCTGCAGSPACI
                     EYCPIEACMFWVADEDHPPFGRIEVDPYLCIGCQKCTSKGPDGAFLDGCPWDAIEMVP
                     TDDWEAVHGIVLPDLPPAPPA"
     gene            complement(5897..6757)
                     /locus_tag="VFH91_00585"
     CDS             complement(5897..6757)
                     /locus_tag="VFH91_00585"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6861499.1"
                     /translation="MHEHQNHSETYGTQAGRRVRASGPILILAFLFLAATFLAWYFTW
                     FGRGLSDADITKYLADERNTRHVQHALLQIQQRIERSDSSARNWYRDVLRLSKSPETE
                     FRLTVAWLMGFDNHNDEFHAALRELVHDPQPIVRRNAALALIRFGDASGRDELLAVLR
                     PYVVKAPYRGLVASTLQNGSNISRGALLARIENDSVLPVEVRSPLPGRIEQIFRTNGT
                     QVGDGEPLLTINSDEESIWEALRGLALIGTENDLELIRSYSASNDASARVKKQAEITA
                     QSITSRSNQK"
     gene            complement(6772..>7005)
                     /locus_tag="VFH91_00590"
     CDS             complement(6772..>7005)
                     /locus_tag="VFH91_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HET6861500.1"
                     /translation="TRFTGQGIARPIYFTILTTHTILAIVIVPLILVTFYRALRGDFA
                     RHRRIARITLPLWLYVSITGVIVYLMLYQIYPS"
BASE COUNT         1693 a         1852 c         1718 g         1742 t
ORIGIN      
        1 agtgtttaag ccgcctgaga actcttgccc aatccgaagc aggcctgaat ctcttccctg
       61 tcgaccgtgt ctcggtccat cagcagttca accaatcgag cgatcgagtc ccgttccgcg
      121 tcgactatct cacaggctcg ctcaagttgg tgactgatca gctcattcgt agcttcttca
      181 atcctatcga gcaacgtttg gctgtgtggc tggtctgatt gtccgaggtg aattggacca
      241 agcttgctca tgccaaactc actgaccatc cgtctggcaa tctccaccgc ccgctcgatg
      301 tcgttggaag cgcccgcagt catcgtcgaa agaatgacct gttcggcagc tcgacctccc
      361 agtaacaacg caatctgatc ttcgagatac tctcgcttct tcatcaagcg atctcgctca
      421 ggcaacgatt gtgtgacgcc tagcgctcgt ccacgaggca gaatcgagac tttatgaatc
      481 ggatcgccat gttcgactaa aagtccgact gcaacgtgac cagcttcgtg aactgcggtt
      541 gcgtaacgtt cgtcttcgtc catggtgaag ccctgacgct cggcacccat caagactcta
      601 tcgcgagcat gttcgatgtg tgggcggccg attgcttcag cattatctcg agttgcacag
      661 attgccgcct cgttcatcag gttcgccagt tcagcaccag aaaaacctgg cgtgccgcgg
      721 gcgatccact gcagatccag accttcttcg agttttaact tgcgggaatg aactttaaga
      781 atctcttccc gtccccgcac gtcagcgaga ttaaccgcga tctcacggtc aaatctgccg
      841 gggcgagtaa gagctttatc gagaatgtcc ggacggtttg tcgaggcaat gacgataact
      901 gtgctgctct gatcaaagcc atccatttca accaacaact gattcaatgt ctgatcttga
      961 tcagcggaag cagaatcgcc tccacgtcca cggcgtcgtc caagcgcgtc aatctcgtcg
     1021 atgaagatga tgcacggcga cagttttcta ccttttgcaa acaacgaccg aactcgtgca
     1081 gcacctaatc ctgcgaactt ctcttgaaat gttgaaccgg acgccgctag aaacggaacg
     1141 ccggcttctt gtgcggctgc tctggccaat agagtttttc cagtgcctgg aggtccataa
     1201 agcagaatgc ctcgaggtac ccgtccaccg agccggctaa atttttcagg attgcgcaaa
     1261 aattcaatcg tttctgtcag ttctgctctg gcttcatcga caccggcaac atcgttgaat
     1321 ccaacttcct gtttgccgtt ctgatcctgc aaatcgaatg atccatgccc actcatactt
     1381 gcatgaactc tccaaccaac gacgcctaaa agcgcgagac tgaagattgg cagaatccag
     1441 ttcagcgcag tcgtgagagg tcccggctgc attggccgaa actcaacggg tacatttttt
     1501 tcgcggaata tctgaaccag ctcatgttga gcagcttcgc ccgttactgt tgccacgaca
     1561 actgacccat caggctttgt aacggaaacg gtctcaccct caatctgcaa ggcttgagcg
     1621 ctgcctgcct ggcctaaggc gtaaagttgg gaatagttaa ttatctgaac ctgttggcgc
     1681 tgacgaaagt aattaatggc ggctatcgaa agaatgacgg taatgataaa aacggtcagg
     1741 cagaccaggg ctactctgcg gctgcgcaga gatacctttg gcgcgggggt aggcataaag
     1801 acctccagaa gatcccaatc ggaaacgggg ttcgattgag ctcgtgatta tttagaactg
     1861 aaaagaatcg ggtcaggaaa gcgacggaac ttcttaggaa gggcccgcac gcgaaaactg
     1921 agaagcggta acgctacgac gttacattga gtcaatgcac tcaatcgaac gccggtgaac
     1981 aatttaacag ccatcgcaac aataagtcca gaagaagatt tccaaaacct ctattcgcta
     2041 tctcttagcg agagcactct cgcctcgaac gtgtttgaca gtaaaactcc acgacccgtt
     2101 gcccgctcac ccatataaaa accgattcac gacaaagata ttgaattaaa gatattgaat
     2161 taaatcactt cgtagaattc gcgagtgact tactttcgta cggagcggct tcatttggtg
     2221 ggataaattc tctgggttta gggcagttac gaacgttcgg gcaatggcat actcgttgct
     2281 gaaccccctc agtgattgct tttgtaccaa acgatcattg gaagcggagc ttgggtaaca
     2341 aaaggaggag aacgaaatgc tttggaccat ttttgtgatt ttgttagtgc tttggctctt
     2401 gggtatggtc agtagctaca ccataggtgg tttcatccac attctgttag tgctggcgtt
     2461 agttgtattg cttatcaaca tcattggagg ccgtcgcacg gtcgtttagg caaactgcac
     2521 actgtttgcc ctgcacagcg tgccagatcg cctggatgag cggtgtaagc tcctgagtct
     2581 caaatttgaa gactaaatag ctggcagatc gagcacgctt gtgattcgcc ctactggata
     2641 aaggctcatt tgtgggtccc agtagggcga acgcctataa aaatatttca ggcgctcata
     2701 agggttctga gcgaattgtg gatcccgctg cagagcttcc tcaaatatat cgttcagttc
     2761 tggattggct gagatcatct ctcgagccag ttgttcgagt acataaggtt cgccgtactc
     2821 tttttcctcg aaaatagcat tgaagaagcc ccaccggacg aaggagtcag gagcctgggg
     2881 ttcgagtaag cccaaaacca gacgcgcaag cggctgatcg acaggcacga ccagcgaccc
     2941 ggcagcaaac tcccttgttt catttgtagg cagcactttg aaacgtggca tatgcctacc
     3001 ttcgaaaggc atgccgggcc attccacatc gacgaagcgg taactctcca ctggaacgct
     3061 cgctgtgttt gtcagccgtt gagtacgtag gccgtgagtc gtcaacacct caatcacctc
     3121 gctccactca actggaacta tataagcaag tggaggaacg acagacgtcg ttacacgaag
     3181 agtttcgtac atcggcacag tgagatctaa tggttcgtcg ccatagatca cccgaagatc
     3241 accggaaatc tcgctttgct cagtttcata actaaacgcc ttcagctcga aaggcgtcgg
     3301 gatatcagtt aattcgaact ccagtggata cttactattt tcggacgctc ttttatcggc
     3361 cttttggcct agttcaatca gccgcttcgg atctttattg acttcgctca acgccgcccg
     3421 caaaaagtca taagttccct tcactcgcga ccggtagtcc ttcagcatgt gcgtctcaat
     3481 caagattcca ggcctgtttc tcaatggtac gtatcctgtt gaaaatctgg gtggaccatt
     3541 gaagtctctc atgccgcgcg gtaggtctcg gttgtcgata aactccaggt accatgagat
     3601 cgtgttaccg gcagcggccg ttgcggcagc caccttgtta cggaatacat cctgttccca
     3661 actaacaacc tctggatcga caccttcgtg gcgctcgaag tgaaaagtta tattcgagcg
     3721 ataatccgcg ccgtcgctta cgtgacaatc gataaacaga tccggttgcc attcattcca
     3781 gagcttgagc caggctcgcg tctcaggagt gtcagccttc atgtaatcac ggttgagatt
     3841 ctgataagta cgcgtagtgc gccatcccat tgagacggga ccattctggt tgatccgatt
     3901 gtaaggactc gacctttcgt gtccatcggt gttgtagata ggaatgaata acaacaccac
     3961 attttcgagt atgcccacaa cggtcttcgt gatagctaga tcgcgaagca aagcaaagcc
     4021 ggcgtccttt ccatcaggtt ccccggaatg gatgcacgct tgaatgagaa ctacggcttt
     4081 tccctgtgat tttgcggctt ccggcgtgaa tgtttgtgtt tcagatgcga ccaggagtgg
     4141 taaatcccga ccctgcccac tcttgccaaa actacgaaac tcgattgtga gcgaagcagc
     4201 cgctaacctg cggcaaaaat cgatagtgtc agcgtagctc ggagtttcgc ggtaatcagt
     4261 ctgttcagcg tgagtcttcc attcggacgg atcattattt gtcattggtc attttccatt
     4321 tgtcattttt cattttgcaa ggacttgcta gttgcattgg actagtagaa cactcggttt
     4381 cgtaccaaat gacaaatgat tccgagttgg ttcccgggtc aactcagtga ggaattacag
     4441 ctgcggcact ctcgccgcct tctggcattg agagtgccgc atgacaaaca acggatgtac
     4501 atcgttcgcg cgaaccaact agtaatgaat gcactgcctc ccaagatcag tatgagaaca
     4561 gctgcattca ggagaaaatc cttcaatccc ttcgtatccc catcgatgaa acgtaaaagg
     4621 agcgaccgcg ttcatacccg tgttggaacg ccgtgtcaag ctcgtcgtct gcatataact
     4681 ctcgcaaatg cggttcaaca tctgcgtaaa gcttgccgcg tctatccgga tgctgagccg
     4741 catggaaccc acgccgataa ctcatttcgt cggtgtcgaa gttctttcca atggttgcgt
     4801 agctttctcg ctcacgatcg cggataggtt gccaccagtt ctcccttaag gtctcttcgt
     4861 cttctgctcc attccgatca aaaatctcac gagccttaga ggcttgagag ccctcttccg
     4921 cataagcaat tacaaccgta tgcccgcgac gcaaggcgtc ttcgtagaga taaacgtctt
     4981 cacgaggtaa gccttcaccc aattcctctt cgagtgtctc gccgactttc gcgcctgcta
     5041 ccgcgccgcc gactccaagc agagctgctc cgagtaaacc gaaggccaat actggcccaa
     5101 cgcctggaat agccagggta gcagctgcga ggccaacggt tgcaccaccg gccgcaccca
     5161 ttgcgcctcc aaccgcggct cccattgcct tacccatacc gggttcttcc gtatcaacag
     5221 ttggtacggc tcgctcaatc tgatgtgggt tgcttcccgg cacaagtagg ccgagtcgct
     5281 ttttagcgat accgatttgt tgtaattgga cgactgtccg ctcagcttct tcttgagatc
     5341 taaatactcc agtgattcct tccattcgcc actccccttc cttattctgt ttacacttcg
     5401 gttcaacgga tttgcagcat ccagcgggcc acaactccca ccgacacaaa tcgttgagga
     5461 aatctgcaga gctatgttag agtccagtta cgaactcaaa tcacgacgga caaaggtgac
     5521 aggatgttcg atattgtaga caagcaatgg ccaatgtcgg tgggtgacgg aaaagccact
     5581 cgaaagaagc ggccgcgaat tctggccatc acaactgact gctgtactgg ttgtgcgggt
     5641 tctccggcgt gtattgaata ctgtcccatt gaagcttgta tgttttgggt tgcggacgag
     5701 gaccatcccc cgttcggaag aattgaagtc gatccttacc tttgcatcgg ctgccaaaaa
     5761 tgcacgagca agggaccgga tggagctttc ctcgatggtt gtccctggga tgcgatagag
     5821 atggttccaa ctgacgactg ggaagcggtt cacggaatcg tgcttcccga cttgcctcct
     5881 gcaccaccag cctaagttac ttttgattac tccgagaggt gatcgactgc gcggtaattt
     5941 ctgcctgttt cttcactcga gctgacgcat cgttcgacgc actgtacgat ctaattaact
     6001 ccaggtcgtt ttcagtccca attagagcaa gcccacgcag cgcctcccaa atgctctctt
     6061 catctgagtt tatagttagc agaggttctc cgtcacccac ctgagttcca tttgtcctaa
     6121 atatttgttc aatccggccg ggcagcggcg aacgaacttc caccggtaat actgaatcgt
     6181 tttcgattcg agcgagtaat gctcctcttg aaatgttgga cccgttctga agggtgctgg
     6241 cgaccaggcc tcgataaggc gctttcacca cataaggtct caatacggca agcaattcgt
     6301 ctcttccgct ggcatccccg aatcttatca acgcgagtgc tgcgttcctt ctcacgattg
     6361 gctgaggatc gtgaactaat tccctgagtg cagcgtgaaa ttcatcattg tgattatcaa
     6421 accccattaa ccaggcaacc gtaagtcgaa actcagtttc cggactcttg ctaaggcgga
     6481 gaacatcgcg gtaccagttg cgtgcactgc tgtcgctccg ttcgatacgc tgctggatct
     6541 gaagcaacgc gtgctgaacg tgccgcgtat tcctttcatc ggcaagatat ttcgtgatgt
     6601 cggcatcgga gagtccgcgg ccgaaccacg tgaaatacca ggctaaaaat gttgcggcaa
     6661 ggaaaagaaa ggccagaata agaattggac cagacgcgcg cacgcgccta cctgcctgcg
     6721 tgccataggt ttccgaatga ttctggtgtt cgtgcattta cagctgaata atcaggaagg
     6781 ataaatctga taaagcatca ggtaaacgat cactccggta atggagacgt agagccaaag
     6841 aggaagcgtg atgcgagcga tacgccggtg tcgggcaaag tcgccacgta aggcacgata
     6901 gaaagtaaca aggattagcg gaacgatgac aattgcgaga atggtatgag tggtgagaat
     6961 ggtgaagtat atgggacgag caattccctg tccggtgaaa cgcgt
//
LOCUS       DASSFP010000026        13901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1196757, whole genome shotgun sequence.
ACCESSION   DASSFP010000026 DASSFP010000000
VERSION     DASSFP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13901
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196757"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFH91_00595"
     CDS             complement(<1..842)
                     /locus_tag="VFH91_00595"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HET6861501.1"
                     /translation="MQGEFRADVSRDTFDASKQFSRVFLQQGRVQLDADWNEQGDIFS
                     RYLRALAADLIGPHGGNAAVAGFNLITTEAEIDNLKDESGNSLPAEIVKSLKEALRSS
                     GFLVGAGRYYVNGTLCINESNEGFIQQPGYPFELNLPLDQIRETPGPYMVFLDVWERH
                     ISSFDDGDITEVALGGAETASRAQVVTSVFVLPPKLGDLNFYDQVQLNTDVTTLKNAI
                     TANNATDIRNARQTLLTSAENTRKRLSIISKAAMRARARVSGMPEGDCNIQPEAQYRG
                     PENQ"
     gene            complement(852..3089)
                     /locus_tag="VFH91_00600"
     CDS             complement(852..3089)
                     /locus_tag="VFH91_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861502.1"
                     /translation="MNKIDRLYELLPPIYRQTDFEQQYALRDLLRVIAGQVNVVESDI
                     RQLYENWFIETCDDWVVPYIGDLIGYEPVHEAGEPGDPRKLESRALNKILTPRREVAN
                     VIGYRRRKGTLALLELLANNVAGWPARAVEFYKLLGGTQSLNHFRSTRSQTANLRNES
                     ILNLINGPFTGSTYTVDVRRANSRYSRGLFNIGSVALFIWRLKSYSVTKTPAYCFEDA
                     GPECYLFNALGHDTQLFNVPAPEPSPTHIANQLNLPTPISRRAFEESRTVVNKKEPKR
                     ASPDYYDRSVAIYLIDRKGDLRLFPREKVIPANLTDWHRYRPAKDSVAVDPVLGRIVF
                     PTNQKPRHGVVVSYNYAFSADIGGGEYERTLTQPDNAKIYRVGRDEQFRTIKAALLAW
                     ENEQAKPQAAVIEIQDSGIYTEQLNVELKSGEYLQIRAAQRKRPIIRLLDYLASLPDA
                     FKVQGQKQSRFIIDGLLVSGRGLQVNGLEDLSNETSVTPSTADETDLCDVTIRHCTLV
                     PGWSLQCDCEPQRPNDPSIELEFTRARVKIDHSITGAITVEAREPAASPEIITISDSI
                     VDATNEENLALSSSRGGIAYTSLSVRRSTIIGQVQTHAIQLAENSIFSGTVTVARRGS
                     GCMRFCYVPPGSRTPRRYECQPDQVTKAVAENLNLSSVDRESALETETERVRPRFNSL
                     RYGNATYCQLSEFCAEEISRGAEDESEMGVFHDLYQPQRAANLRARLDEYTPAGTDAG
                     VVYAN"
     gene            complement(3086..6754)
                     /locus_tag="VFH91_00605"
     CDS             complement(3086..6754)
                     /locus_tag="VFH91_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HET6861503.1"
                     /translation="MSCDCGCTGHLPCGCCEGTQILTPAQLGNRPGLNAISYRAGTHG
                     SFLTTMKAGLSGNKLSPLTKLKTRDTGDFSIALMDCWATVGDVLTFYQERIANEGFLR
                     TALERRSVLELARLVGYVLRPGVAASTYLAYSLDKPISIPALIDVNAVSTAPPDPPVT
                     IPAGSKAQSVPGPGELPQTFETSNDLIARASWNNLQVRLSRPQKITENTRTIFFKGSP
                     NLKPGDPLLLLIYASPPKPYLFRVESVTPDPPNDRTRVNISPWLRDDEDVSDTAPGAP
                     FHQVVEEIIVRFSAVENFGVSATSRSGAETLRILEQLKSQLASTTDEPVLAAFVRRQT
                     MPKLQEQHDAAVTGRFTRVEPWLAALINALERAVKNEMSTRISPGGSSGASPLPKPKG
                     VTTLGAVLSTLTKAPSIQPQSQLDLSRSVTSSLAPNGDLATRLLTELKPELESALYKA
                     WSNVPVSTGPNYEVHTFRTRASLFGHNAPLEPVKNDAGVITDTKEWDLHYFSISSTEP
                     FRIEMRSDQGHQQFTGVITVGSHSSEQQTFNIGENTVEITAADEKVMVDVKRVTDTVF
                     VIRFEFASRPIVLSVGLTFGQSNLSVDSSESGSGVSITDSTLTTSGSLTIKGQLQTTA
                     GRQSKEAANVVWLDAAYDRIKPDSHDDENRTPPDAQSWIVLERPEAVVDADGTTFIPK
                     LVISRVLQASERSRADYGIAIKSTRITLDKNWIDVRASDGFEVVRETAVFAESEKLEL
                     AEEPIEDDISGGELELDGLYDGLESGRWLIVSGERTDVGSNGSQTSGQQTPTRVTGVP
                     ATELVMLAGINQRYDANLPGDKTHSFLALANSLAYSYKRDTVVVYGNVVHATHGETRQ
                     EVLGNGDAAKSLQQFTLKQSPLTFISAATPAGAETTLLVRVNDVLWRETDTLAALGPN
                     DHRYITRTDDDDKTSIVFGTGQNGSRLPTGPENITAKYRNGIGTPGNVRADQITLLTS
                     RPLGVKGVTNPLPGTGGADREDRDQARRNAPLAVTSLDRLVSTQDYADFARTFAGIAK
                     ASATRLSDGQRRVVHLTIAGNDDIQIQPTSDLYRNLIKALRDFGDPFVPVQLVMRSLR
                     LLIVEAAVRVRPEYLWEKVEPKIRAALLDRFGFKNRDLGQDVLLSDVISTIHRVEGVA
                     YADVNEFDSVDADSIVSQLSGGDALSDTITLRERIPVDLARIDFTETDASKRIRPAEL
                     VYLTPDVTDTLVLSEITS"
     gene            complement(6751..9393)
                     /locus_tag="VFH91_00610"
     CDS             complement(6751..9393)
                     /locus_tag="VFH91_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HET6861504.1"
                     /translation="MDDVTLQCSDEHRRQLMRDQADEKGGLNGVDFVEVVAGTSQREL
                     CVHFFGEIPQLVRNNVRIQGGSRIRDIKVLEIQPHESADPEHQDCLRVLLDKPGDFSC
                     YRICFYEVDNNGLTTNSYLKNFDPRYACAEFSFKVDCTSELDCLPKAECLPTELTTPE
                     INYLAKDYASFRQMMFDRMSLLVPDWQERHVPDIGVALIEILAYVGDYLSYYQDAVAT
                     EAYLDTARQRISVRRHARLVDYFLHEGCNARAWITVWTDQDLNDPSLTLRDLYFATSE
                     DRDAEIFEPLVPDPAKPLELYTAHNKIRFYTWSNQECCLPRGSISATLLDDWIPEKTD
                     YKSQSNKQVADYPANQSELPRSLGHLKVGDVLIFEEVIGPRTGNEDDADHSHRHAVRL
                     TSVTATVDPLIQIDVPGASQKLPTPVVEIEWSIEDALPFHLCLSARLPIPDCSLVTDI
                     SVARGNVILVDHGQTIDEPEDLGQVGQLEITGECACDGNVMDLTTVPAKFAPVLLGQP
                     LTFSAGINWSAPAAVAIMQDPRNALPQLLHVIGLPGKCPEPGSFQQPEVLEGIDATAE
                     SWKWYSRRDLLSSGSLDQHFVVEMDNDGLAHLRFGDGELGRMPAPCSLFTADYRIGNG
                     TSGNVGADTITTIGWRKETVGGLILKPRNPLPATGGVDREPMAEAKLFAPRAFLKRLE
                     RAITADDYARIAERFPGNRVQRAGAALRWTGSGYVAQVSIDTVGSEMADPQLLNDIKD
                     YLYPYRRIAHDIEVQQARYVPLRIEISICVSPHYLRGDVEEALLNVFGNRRLPDGSYG
                     LFHPDNLTFGEGIYLSKLIAAAQAVTGVESVRVTALERLGDGPRAELEEGILKLGPLE
                     VAQLDNDPNFPERGILKLTMRGGR"
     gene            complement(9393..9755)
                     /locus_tag="VFH91_00615"
     CDS             complement(9393..9755)
                     /locus_tag="VFH91_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HET6861505.1"
                     /translation="MTNIDFPFQFNARGRTATTDDDDHVRDMIEQLLFTNAGERVNRP
                     DFGSGLLQLIFAPNSPEMAAALQFTLQAALQQWLGDLIQIQALEVESEDSSLRVGLQY
                     VVLRTAEQRTTTFVRGGV"
     gene            complement(9787..10107)
                     /locus_tag="VFH91_00620"
     CDS             complement(9787..10107)
                     /locus_tag="VFH91_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861506.1"
                     /translation="MPGFLVHLGATVQCSHFAPAQPTAPNPRVLVSGQPTVTMPTPYA
                     IAGCLFNVSGAPSPCVTGQWITGSTRVFSNGQPLVLLDSQSVCAPNGTPMLIIATQTR
                     VIAT"
     gene            complement(10120..10641)
                     /locus_tag="VFH91_00625"
     CDS             complement(10120..10641)
                     /locus_tag="VFH91_00625"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HET6861507.1"
                     /translation="MSDSTPKYYGMYRGTVINNIDPMQMARIQVIVPDTGGLTPSTWA
                     MPCVPVAGKQSGTFVVPQIGAGVWIQFENGDPDYPVWTGGWWGSAAEVPAIALAGNPV
                     SPSIVLQSGLQNMLMISDVPGPTGGIILKTTLGAFISINDATGITISDGKGGMITMIG
                     GIVTINQGAILIK"
     gene            complement(10638..11765)
                     /locus_tag="VFH91_00630"
     CDS             complement(10638..11765)
                     /locus_tag="VFH91_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861508.1"
                     /translation="MNQGFYLSIRMGSVNPTAVPQQVIEALTEVSVSSTVGAQSGFQM
                     KFTLGKNSFISQNLLPSGFFDPRTRVIITVTVNGSPEVLMDGIITKHDVTPSNEPGKS
                     TLTVTGLDISALMDFIDLTGIPYPALPNFLIVDIVLAKYLLFGVVPVVIPDLVNILSN
                     PLEKFPKQQGTDYRYVSTLGQKSGYVFYIDPGPEPGMSLAYWGPEFGGFLPTQPALSI
                     NLDAATNVESLNFSYNGLAATQYIATILEKNSKIPIPIPIPSIDFLKASLSEKAPTPL
                     KSKVISDLASKSFGEAALLVVGALMATDDAITGSGSLDVLRYGHVLKARRKVAVRGAG
                     IYYDGLYNVKSVTHNMKRGEYKQNFTLARGGVKSSVSTVGL"
     gene            complement(11762..12121)
                     /locus_tag="VFH91_00635"
     CDS             complement(11762..12121)
                     /locus_tag="VFH91_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861509.1"
                     /translation="MSDALQALITANSIRATPYPINSRYYGVAVVQFEDTAGRAVVYL
                     RRRFIPQADRFHLLQFHTVTEGERLDNITARYLGDPEQFWRVCDANNAMRPQELTEKI
                     GRQLRITLPEGISGPTL"
     gene            complement(12118..12774)
                     /locus_tag="VFH91_00640"
     CDS             complement(12118..12774)
                     /locus_tag="VFH91_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861510.1"
                     /translation="MSSFPNSPYVIKGGIVLLDPTTAQVQKIIALQYNPDTLTRSLQV
                     QAVTEGSDRSEALRLKGPPVETFKVEAEIDATDQLEAAGQNRTVVENGIHPQISALEM
                     IVYPRSTQAIANFALSQTGTLEIMPVEAPLTLFVWSARRILPVRLTEFSITEEAFDTK
                     LNPIRAKVSLGMRALSFDDLGFATKGGSLFLAYQQQKERLAALSQGSSFGTLGIGGIP
                     "
     gene            complement(12771..>13901)
                     /locus_tag="VFH91_00645"
     CDS             complement(12771..>13901)
                     /locus_tag="VFH91_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861511.1"
                     /translation="HELAHVVQNDNVGGSAPTAALESEAEIAADSVVSGKQPDVQHAS
                     SQPALAITRGEKTAAGAALGGAIGGAAGGLLGLGIASKMSGTPFLSGLAIGGLVGMGV
                     GALVGGLIGFFTRRHESESVAEAEELIRRKYGKYIPNAKMGPLHNASVHVVTKEELCE
                     RKRCRTPEADCDLIGWTDTGVPMKPSKGPADQPAPIAKPEDEPTCNNKQMEHATVDHP
                     VIYYVKNDETAGTLIHEGLHAHSHPSFGYLHNHVVEGTTEYFSRKLQDEINMPHYGSY
                     NDEVQSVEKLVDLVGEERLAQGYFTGMMPELHQAVNSKLGQCALITWAFNLQMGSEAR
                     AQQIMALRNQNYCQSNELNTVSPSSLTPAAPAKDQTQQTEQK"
BASE COUNT         3183 a         3693 c         3503 g         3522 t
ORIGIN      
        1 aattgattct cgggtccgcg atactgtgct tcaggttgaa tattgcaatc gccttccggc
       61 ataccactaa ctcgagctcg cgctcgcatg gctgccttac tgattatcga caatcgtttt
      121 cttgtgtttt ctgcggacgt cagtagcgtc tgtcgagcat tcctgatgtc ggtcgcattg
      181 tttgcagtaa ttgcattctt gagcgtagtg acatcggtgt tgagctgtac ctgatcgtag
      241 aagtttagat caccaagttt aggcggcagc acaaacactg aagtgacaac ctgtgctcga
      301 ctcgcagttt cagcaccacc caaagcgact tcggtgatgt ctccgtcgtc aaacgatgaa
      361 atgtgtcgtt cccaaacgtc gagaaacacc atgtaaggtc ccggcgtctc cctaatctga
      421 tcaagtggaa gattcagctc gaagggataa cctggctgct gaatgaagcc ctcgttactc
      481 tcgttgatgc aaagcgttcc gttaacatag taacgacccg caccaaccag gaaaccactg
      541 gagcgcaacg cctctttcag actcttcaca atctcagccg gcagactgtt tccgctttcg
      601 tccttaagat tatcgatttc tgcttcggtt gtgatcaaat tgaagccggc aaccgctgcg
      661 ttgccgccat gtggaccaat caggtcagcc gcaagagctc tgaggtaacg tgaaaagatg
      721 tcgccttgct cattccaatc cgcatcaagt tgcactcttc cctgctgcaa aaagactcga
      781 ctgaactgtt tgcttgcgtc gaaagtatct cggctaacat ctgctctgaa ttcgccttgc
      841 atgatttcct cctaattggc gtaaacaacg ccggcatctg ttccggcggg ggtatattcg
      901 tcgaggcgcg cccgtaagtt ggcagcccgt tggggttggt aaagatcgtg gaacactccc
      961 atttccgatt cgtcttccgc accgcgcgaa atctcttcag cacaaaattc tgagagctga
     1021 cagtaagtgg cattcccata tcgcagactg ttgaacctgg gacgcactcg ttccgtttct
     1081 gtttccagag cactctctcg atcaacactt gagaggttta gattctctgc tacagctttt
     1141 gtgacttgat ccggttggca ttcataacga cgcggcgtac gagatcccgg cggcacgtaa
     1201 caaaaccgca tgcagccgct tcctcgacgg gcaacggtca ctgttccaga aaagatcgag
     1261 ttctcggcca attgaatggc atgagtttga acctggccaa tgattgtcga tcgcctgaca
     1321 ctcaatgacg tataagcaat tcctccgcgg gaactggaaa gcgcgaggtt ctcttcattt
     1381 gtggcatcga cgatgctgtc gctgatcgta attatttcgg gagacgctgc cggttcacgg
     1441 gcttcaactg taatagctcc tgtgatgctg tgatcaatct tgacgcgggc tcgtgtgaac
     1501 tcgagctcga tgcttggatc atttggccgt tgtggttcgc aatcgcactg caaactccag
     1561 ccaggaacca gcgtgcaatg acgtatggtc acatcgcaaa gatcagtttc atccgcagtc
     1621 gagggtgtca cactcgtctc attactgaga tcctccagac cattcacttg cagcccgcga
     1681 cctgaaacga gcaatccgtc gatgatgaag cgactttgct tctgaccctg gaccttaaat
     1741 gcatctggaa gacttgcgag ataatcgagc agtcggatga tcggtcgctt tcgttgcgca
     1801 gcgcgaatct gaagatattc tccactcttt agttcgacgt tgagttgctc tgtatagatc
     1861 ccgctatcct gtatttcaat cactgctgct tgaggcttcg cctgttcatt ctcccatgcc
     1921 agcagcgcag ccttaattgt tctaaattgt tcgtcccgcc cgacacgata gatctttgca
     1981 ttgtccggtt gcgtcagagt gcgctcatat tcgccgcctc cgatgtccgc actaaacgcg
     2041 tagttataag agacgactac tccgtgtcgt ggcttttgat ttgtaggaaa cacgattcgt
     2101 ccgagcacag gatctaccgc tacagaatcc ttcgccggac gatacctgtg ccaatcggtg
     2161 agattcgccg gaattacctt ctcacgcgga aataatcgca agtcgccttt gcgatcgatt
     2221 agataaatcg caacgctacg gtcgtaataa tcaggagatg cgcgctttgg ctctttttta
     2281 ttaacgactg ttcgtgattc ttcaaaggcg cggcggctga ttggcgtcgg caaattaagt
     2341 tgattagcga tatgcgtcgg cgagggctcg ggtgccggta cattgaaaag ttgagtgtcg
     2401 tggccgagtg cattgaagag atagcattct ggaccggcat cttcaaaaca gtaagctggg
     2461 gttttagtaa cggagtacga cttgagcctc cagatgaata acgcgacact tccgatatta
     2521 aatagaccgc gcgagtaacg agagttcgca cgcctaacat ccaccgtata agttgaaccg
     2581 gtaaacggtc cattgatgag gttcaagata gattcatttc ggaggtttgc ggtctgcgat
     2641 cgagttgagc ggaaatgatt aagactttga gttccaccga gcagcttata aaactcgact
     2701 gctcgcgcgg gccagcctgc aacattgttt gcaagcagtt cgagcagggc cagcgtgcct
     2761 ttccgacggc gatacccgat gacgtttgcg acttcacgcc tgggagtcag aattttattc
     2821 agcgctcgcg actccagctt ccgaggatcg ccgggttcgc ctgcttcatg aacaggttcg
     2881 taaccaatca ggtcgccaat gtacggcaca acccaatcat cacaggtttc gatgaaccag
     2941 ttttcgtaga gttggcggat atcgctttca acgacattca cttgcccggc gatcacacgc
     3001 agtagatccc gcaaagcata ttgctgctcg aaatccgttt gccggtagat cggaggtaac
     3061 agttcgtaaa gccggtcaat cttgttcatg aggtgatctc cgaaagaacg agcgtgtcag
     3121 tgacatcagg ggttagatag accaattcgg ctggcctgat ccgttttgac gcgtcagttt
     3181 cagtgaagtc gattcgcgcg agatcaacag gtatgcgttc gcgaagtgtg attgtatcgc
     3241 tcaacgcgtc accaccactg agctggctga caatactgtc agcatccacc gagtcaaact
     3301 cgttgacgtc tgcgtacgcg actccttcga cacggtgaat cgtgctaatt acatcgctta
     3361 aaagcacgtc ctggcccagg tcgcggttct tgaaaccgaa ccgatcaagt agcgctgccc
     3421 tgattttcgg ttcgaccttt tcccacagat actccgggcg aacacgaact gctgcctcaa
     3481 cgatcagcag tcgcagactt cgcatcacga gttgaactgg aacgaaagga tcaccgaagt
     3541 ctcgcaacgc tttgatcagg ttgcgataaa gatccgaagt tggctgtatt tgaatgtcgt
     3601 cattcccagc gatggtcaaa tgcaccactc gtctttgccc gtctgacaaa cgagtcgcac
     3661 ttgccttagc gattccagca aaagtgcggg cgaaatctgc gtagtcttga gttgatacaa
     3721 gtcgatcgag agaagtaact gccaggggtg cgttacgtcg agcttgatca cgatcttctc
     3781 tatccgcgcc gccagtacct ggtagcggat tcgtaacacc tttcacgccg agcggacgcg
     3841 aagtaagcaa agtaatctga tccgctcgca cattgcccgg cgttccgatt ccgttccgat
     3901 attttgcagt tatattctct ggtccagttg gtaaacgcga accattttgc ccggtgccaa
     3961 atacaatcga cgtcttatca tcatcgtcag tcctggtgat gtatcgatgg tcatttggtc
     4021 ctaacgccgc gagtgtatct gtctcacgcc aaagaacatc gttgactcgt accaggagtg
     4081 tggtttctgc accggccggc gtagctgccg aaataaaggt tagaggagac tgtttcagtg
     4141 tgaactgctg tagagacttt gcggcatctc cattcccaag tacttcctga cgcgtctcgc
     4201 cgtgagttgc gtggaccaca ttgccgtaaa cgacaactgt gtcgcgtttg taagagtaag
     4261 ccagcgagtt tgcgagcgcg agaaacgaat gagtcttatc gccgggaagg tttgcgtcgt
     4321 atcgttgatt gatccctgca agcattacca gctcagttgc tggaacaccg gtcactcgag
     4381 ttggtgtttg ctgcccgcta gtttgagaac cgtttgaacc tacgtcggta cgctcgccgc
     4441 tgacgatcaa ccatcgtcca ctctcaagcc catcatagag tccatctaat tccaattcgc
     4501 cgcctgaaat atcgtcctcg attggctctt ccgccagttc caacttctca ctttctgcaa
     4561 aaaccgctgt ttcgcgtacc acctcaaaac cgtcagaagc ccggacgtca atccagttct
     4621 tatccaatgt tatgcgtgta ctcttgatcg cgattccata atcggcgcgg gaacgttcac
     4681 tggcctgaag aacccgactg atgacgagtt ttggaataaa cgtggtgccg tccgcatcta
     4741 ctaccgcctc gggcctttca agcacgatcc agctctgcgc atcaggtgga gtacgatttt
     4801 cgtcgtcatg actgtctggt tttatgcggt cgtatgcagc gtctaaccac acaacattcg
     4861 cagcttcttt actttggcgg cctgctgttg tttgcagttg tcctttaatc gtcagggaac
     4921 ccgaggtcgt gagcgtgcta tcggttattg agacgcccga cccgctctcg ctgctatcaa
     4981 ctgataggtt ggactgtccg aaagtgaggc caacggataa gactatcgga cgagatgcga
     5041 actcaaatcg aattacgaac actgtatccg taacgcgttt cacatccacc atgaccttct
     5101 catccgcagc agttatttca acagtatttt cgccaatgtt gaaagtctgc tgctcgctcg
     5161 aatgactacc gacggtaatg acgccagtaa attgctgatg accctggtcg gagcgcatct
     5221 cgatcctgaa cggttcagtg ctcgagatgc tgaagtaatg cagatcccac tctttggtat
     5281 cggtaattac acccgcatca tttttaaccg gctctagcgg agcgttatga ccgaagagcg
     5341 aagctcgcgt tcgaaatgtg tggacctcat aatttggacc ggtcgaaact ggaacattgg
     5401 accatgcctt gtacaaagcc gactctaact caggttttaa ctccgtcagc agacgtgtag
     5461 cgagatctcc atttggggca agagagctcg tgacactgcg tgaaagatcg agttgactct
     5521 gtggctgaat agatggggct tttgtgagag ttgataagac tgccccgaga gttgtaacgc
     5581 cttttggttt aggtagaggc gatgctccgc tgcttccacc cggcgaaatg cgcgttgaca
     5641 tctcattctt gacagctctc tcaagcgcgt ttatcagtgc ggccagccat ggttcaactc
     5701 gagtaaatct tccggtcaca gcagcgtcgt gctgctcctg cagtttcggc attgtctgtc
     5761 gccggacaaa cgcagccagg actggctcat ctgtagtcga agcaagttga gatttcaatt
     5821 gctcgaggat gcgtagagtt tcggcacccg atcgacttgt cgctgaaacc ccaaaattct
     5881 caaccgcact aaatcgaaca atgatctctt ctacgacctg gtggaaaggt gcacccggag
     5941 ccgtatcgga aacgtcctcg tcatcacgta accagggact gatatttacc ctggtacgat
     6001 cattcggtgg atcaggtgta acactttcaa ctcggaatag gtacggtttt ggcggactcg
     6061 cgtaaatcaa caacaacagg ggatcgccgg gctttaaatt gggactaccc ttgaaaaaga
     6121 tcgtgcgagt gttttctgtg atcttttgcg gccggcttaa acgcacttgt aagttattcc
     6181 atgaggctcg cgctattaag tcgttagatg tttcaaatgt ttgcggtaac tcacctggtc
     6241 caggaacgct ttgcgcctta cttcctgccg gtattgtgac aggaggatcc ggcggggcag
     6301 tggacactgc attcacgtca atcagggcag gaatagagat tggcttatcg agtgaatagg
     6361 cgagataagt gctcgctgcg acgcctggtc tcaatacata gccaaccagt cgcgccaact
     6421 ccaaaactga tcggcgttcg agagcagtcc gaagaaatcc ttcgttagca atacgttcct
     6481 ggtaaaacgt gaggacatct ccgaccgtcg cccagcaatc catcaaggca atcgagaagt
     6541 cgccggtgtc gcgcgttttc agttttgtta gtggcgataa tttgttgccg gagaggcccg
     6601 ccttcatggt tgtcagaaaa gacccatgag tgcccgcccg gtagctaatg gcgttcaaac
     6661 cgggccggtt acccaactga gcaggcgtca ggatttgagt cccctcacaa catccgcaag
     6721 gtaaatgacc ggtacatccg cagtcgcaac tcatcgtcca cctcgcatcg ttaatttcaa
     6781 tattcctcgt tccgggaagt ttggatcgtt atcgagctgg gcaacctcaa gtggtccaag
     6841 tttcaatatc ccctcttcca attcagcacg agggccatca cctaatcgtt caagcgcagt
     6901 gacgcgaaca ctttcgacgc ccgtcaccgc ctgggctgcc gcaatcaact tgctcagata
     6961 aataccttcg ccaaacgtca ggttgtccgg atgaaataga ccataactac cgtcgggtaa
     7021 ccgccggttt ccaaatacat tcagcaacgc ctcttcaaca tcgccgcgca agtagtgagg
     7081 tgagacgcag attgagatct cgattcgcaa gggcacatat cgcgcctgtt ggacttcgat
     7141 gtcgtgtgca attcggcgat atggatagag gtagtctttg atgtcgttga ggagttgcgg
     7201 atcggccatt tcgcttccta cggtgtcgat ggagacctgg gccacatacc cacttccggt
     7261 ccaacgcagt gcggcgcccg cacgctgcac tcggtttcct ggaaatcgtt ccgctatccg
     7321 cgcatagtcg tcggcggtga tcgcgcgttc gagtcgcttc agaaaggccc gcggggcaaa
     7381 tagcttcgct tccgccattg gctctcgatc gactccaccg gtcgcgggca gaggatttct
     7441 gggtttaaga attaagcccc cgaccgtctc cttacgccat ccgattgtgg taatcgtgtc
     7501 tgctccgaca tttcccgagg tgccgttccc gattcgataa tcggccgtga aaagtgagca
     7561 gggtgcaggc atccgaccaa gctcaccatc tccgaatcgc aggtgggcca gcccatcgtt
     7621 gtccatctcc accacaaaat gctggtccaa tgagccgctg ctcaaaagat ctcgcctcga
     7681 ataccacttc cagctttcgg cagttgcgtc gataccttcg aggacttcgg gttgttggaa
     7741 gcttccgggc tctggacact ttccaggaag tcctatcaca tgcaacaact gcggcagggc
     7801 atttcgcgga tcctgcatta tggcgaccgc cgctggcgca ctccaattaa ttcctgcgct
     7861 aaacgtgaga ggctggccca gaagaaccgg cgcaaacttt gctggaactg tagtcaggtc
     7921 catgacattg ccatcgcacg cacattcgcc agttatttcg agttgtccaa cctgtccaag
     7981 gtcttccggc tcgtcgatgg tttgaccgtg gtcgacaagt attacgtttc cgcgagcaac
     8041 gctgatatct gtaacgagag agcagtccgg gatcggaaga cgcgcagata gacataagtg
     8101 aaatggcagc gcgtcttcaa tcgaccattc gatctcgacc accggcgtgg gcaatttttg
     8161 ggatgcgccg ggcacgtcaa tttggatcaa cgggtcgaca gttgcagtga cactggtgag
     8221 acgcaccgcg tgacgatgcg agtgatccgc gtcgtcctcg tttcctgttc gaggtcctat
     8281 cacctcttcg aagatcaaga catcaccgac tttcaaatga cctaaacttc gcggcaactc
     8341 tgattgattg gctggatagt ccgcaacctg cttattcgac tgcgatttgt agtcggtctt
     8401 ctcaggaatc caatcatcca gcaaagtagc cgagattgag ccgcgtggta gacagcactc
     8461 ctgattgctc cacgtgtaaa acctgatttt gttatgtgcg gtgtagagct ctagcggctt
     8521 ggctggatca ggaacgagag gctcaaagat ttcggcgtcg cgatcttcgc ttgtcgcaaa
     8581 atagagatct cttaaagtca aagacggatc attcagatcc tgatcggtcc aaactgtaat
     8641 ccacgctcgt gcgttgcatc cttcgtgtaa gaagtaatca accagacggg catgtcgtcg
     8701 aactgagata cgttggcgcg ctgtatcaag ataggcttca gtcgcaaccg catcctggta
     8761 gtaactgagg taatctccga cgtacgccag aatttctatc aaagccacgc cgatatcggg
     8821 cacatggcgt tcctgccaat caggcaccag caatgacatc cgatcaaaca tcatctggcg
     8881 aaagctggcg tagtcttttg cgagataatt gatctcgggc gttgtaagtt cagttggtag
     8941 acattcagcc ttaggaagac aatcgagttc gctcgtacaa tccaccttga aactgaactc
     9001 cgcgcaggca tagcgaggat cgaaattctt caaatacgag ttagtggtca gtccgttgtt
     9061 atcaacttca taaaagcaga tgcgataaca ggagaagtct cctggtttat cgagcaacac
     9121 tcgcaaacaa tcctggtgct ccggatcggc tgactcgtgc ggctgaattt ccaatacttt
     9181 gatatccctg atgcggctcc cgccttgaat gcgaacgtta ttacgaacga gttgcggaat
     9241 ttcgccaaag aagtggacgc acaattcacg ctgcgatgtt ccggcgacga cttcgacgaa
     9301 gtcgacgcca ttaaggccgc ctttttcatc ggcctggtcc ctcatgagtt gacggcgatg
     9361 ttcgtctgag cattgcagcg tgacgtcgtc catcacacac ctcctcgcac gaatgttgtc
     9421 gtacgctgtt ctgccgttcg caacacgaca tactgcaatc cgactcgcaa gctggagtcc
     9481 tcactttcca cttccagcgc ctgaatctga attagatctc ccaaccattg ttgcaatgcg
     9541 gcctgtaagg tgaactgcag agcggcagcc atctcaggac tgttgggcgc gaagatcagt
     9601 tgaagtagac cactgccaaa atcaggacga ttcactcgct caccagcatt cgtgaacagg
     9661 agctgttcga tcatgtcgcg aacatggtcg tcatcgtccg tagtggctgt gcgtccgcgc
     9721 gcattaaatt gaaagggaaa atcaatgttc gtcataaacg tagctgcgct tcgcgcagcc
     9781 tcctgatcaa gttgctatca ctcgagtttg agtcgcgata atcaacatcg gcgttccgtt
     9841 cggcgcacag actgattgac tatccaacaa cacaaggggc tgcccgttgg aaaacactcg
     9901 ggttgatcct gttatccatt gtccagttac gcatggcgac ggcgctccac tcacgttgaa
     9961 cagacaaccc gcaattgcat atggggttgg cattgtcacg gttggctgac cactgacaag
    10021 cactcttgga ttgggcgccg tcggttgggc tggggcgaaa tgtgagcact gcaccgttgc
    10081 tccgagatgg acgagaaatc ctggcatttt tagtctcctt tacttgatca gtattgcacc
    10141 ttgattaatt gtgacgatgc ctccaatcat ggtgatcatt ccgcctttgc catcagagat
    10201 agtgattcca gtggcgtcgt tgatcgaaat gaacgcgccc agggtcgttt tcaaaatgat
    10261 cccgccggtt ggaccaggca catcgctaat catcaacatg ttctgcaggc ctgactgcaa
    10321 gacaatgctt ggactgactg gattaccggc aagcgcaatg gccggcactt cagcggctga
    10381 cccccaccaa cctccagtcc aaactggata atcgggatcg ccattctcaa attggatcca
    10441 aacgccggcg ccaatctgag gtacgacaaa cgttcctgac tgttttccgg cgacaggtac
    10501 gcaaggcatg gcccaggtcg atggcgtcag tccgcccgta tccggaacga ttacctgaat
    10561 gcgcgccatc tgcatcggat cgatgttatt gatcacggtg cctcgataca tgccgtagta
    10621 tttgggtgtg ctatcgctca tagtcctaca gttgaaacag acgacttcac acctccgcgg
    10681 gccagggtga aattctgttt gtactcgccg cgtttcatgt tatgagtcac gctcttaacg
    10741 ttataaaggc cgtcgtaata aatcccggct ccgcgtaccg caacctttct tctcgctttc
    10801 aaaacatgcc cgtagcgcaa aacatcgagc gaacccgatc cagttattgc atcgtcggtc
    10861 gccatcaatg ctccaaccac cagtagtgcg gcttcgccga aactcttgct cgcaagatcg
    10921 ctgatgactt tactcttcag aggcgttggt gctttttccg acagcgaggc tttcagaaag
    10981 tcgatacttg gaatgggaat agggatcggg atcttcgaat tcttttcgag aatggtcgct
    11041 atatattgtg tcgcggccaa tccgttgtac gagaaattca aagactcaac gttggtggcc
    11101 gcatcaagat tgatgctcaa cgcaggttga gtcggtaaga agcctccaaa ttccggaccc
    11161 cagtaagcga ggctcattcc cggttcagga ccaggatcaa tgtagaaaac gtagccactc
    11221 ttctggccca gtgttgatac gtagcgatag tctgtgcctt gttgctttgg aaatttctcg
    11281 agtggattac tgagaatgtt aaccaggtcg ggaattacaa ctggcacgac tccgaacagt
    11341 aagtactttg caagaactat atccacgatc aggaagttgg gaagcgctgg atatggaatg
    11401 cctgtcagat cgatgaaatc cattagagcc gagatatcca aaccggtcac tgtcaacgtc
    11461 gacttgcctg gctcgttgct gggagtcacg tcgtgcttgg tgattattcc gtccattaaa
    11521 acttcgggcg agccgttaac tgtgacagtg ataattaccc tggtcctggg atcgaaaaac
    11581 ccggagggta agaggttttg gctgatgaac gagttcttgc cgagcgtgaa cttcatttga
    11641 aatccactct gcgcgccgac cgtggaactg actgacactt cagtcagcgc ctcaatcacc
    11701 tgctggggca ctgcggtcgg gttcacggat cccatcctta tcgatagata aaagccctgg
    11761 ttcataatgt tggaccagaa attccctcag gaagtgttat tcgtagttga cgtcctatct
    11821 tttcggtgag ttcctggggt cgcatagcat tatttgcatc gcatactctc cagaactgtt
    11881 ccggatcgcc aagatatcta gctgtgatgt tgtccaaccg ttcaccttcc gtcaccgtgt
    11941 gaaactgcaa aaggtgaaaa cgatcagctt gcggaataaa acggcggcgt aagtagacga
    12001 ccgcccgtcc tgctgtgtcc tcgaactgca caactgcaac gccgtagtag cgactgttga
    12061 tcggataagg agtagcccga attgaattgg cagtgatcag tgcctgcagt gcatcactca
    12121 tggaattcct ccaatgccaa gggttccgaa actgctgccc tggcttaaag cagcgagacg
    12181 ttctttttgc tgctgataag caagaaagag actgccgcct ttagtcgcaa atccgagatc
    12241 atcgaaacta agagctcgca ttcccaggct caccttggcg cgtatcggat tcagctttgt
    12301 atcaaacgcc tcttcagtaa tgctgaactc agtcaaacgc acaggtagaa ttctcctggc
    12361 actccacacg aacagtgtga gaggcgcttc gacaggcatg atttccaatg tgccggtttg
    12421 agaaagcgca aagttggcaa tggcctgcgt actcctggga tacacgatca tttccagtgc
    12481 agagatctga ggatgaatcc cgttctctac gacggtccta ttttgtcccg ctgcttctaa
    12541 ctggtccgtt gcgtctatct cagcttcgac tttgaaggtc tcaaccggcg gccccttcaa
    12601 acgaagtgct tccgaccgat cgctgccttc cgtcaccgcc tgaacctgca gagaacgcgt
    12661 gagcgtgtca gggttgtact gcagcgcgat gatcttctgg acctgtgccg tagtgggatc
    12721 gagcagcaca atgccacctt taatgacgta tggtgaattg ggaaagctac tcatttttgt
    12781 tccgtctgct gagtctgatc tttagcagga gcggccggag ttaaactcga cggcgacacc
    12841 gtattgagtt cgtttgattg gcaataattc tgattgcgca atgccatgat ctgttgtgca
    12901 cgggcttcag atcccatttg taaattgaac gcccaggtga tcagcgcgca ttgccccaac
    12961 ttgctgttca ccgcttgatg caactccggc atcattccag tgaagtaacc ttgcgcaagt
    13021 ctctcttctc caactaagtc aaccagtttt tcaacgctct gaacttcgtc gttgtagctg
    13081 ccgtagtgag gcatgttgat ctcatcctgt agcttgcgag agaaatattc cgtggtgccc
    13141 tctacgacat ggttgtgtag atatccaaag cttgggtgcg aatgggcatg caatccttcg
    13201 tggatcagtg ttcccgccgt ctcgtcattc ttcacgtaat aaatgactgg atggtccact
    13261 gttgcatgct ccatctgttt gttgttgcag gttggctcgt cctcaggctt ggcgatcggc
    13321 gcaggttgat cggcaggtcc tttactcggc ttcattggta ctcctgtgtc ggtccaacct
    13381 attagatcgc agtcggcctc aggtgttcga catcgcttgc gttcgcataa ttcttctttg
    13441 gtgaccacgt gaacacttgc gttgtgcagt gggcccattt tggcgtttgg tatgtacttt
    13501 ccgtatttcc ttctgatcag ttcttccgct tcagcaacgc tttctgattc gtgtcgtcta
    13561 gtaaagaacc cgatcaaccc tccgacgagc gctcccacgc ccatcccgac cagtcctcca
    13621 atggcaaggc cactgagaaa aggagtgcca ctcattttcg aggcgatgcc caaacccagt
    13681 aatccacccg cagcaccacc tatagcacca cctaatgcag cacccgctgc cgtcttttca
    13741 ccccgggtta ttgcgagggc cggctgagaa gaagcgtgtt gaacgtcggg ctgttttccg
    13801 ctgacgactg aatcggctgc gatctcggct tctgactcaa gcgcagccgt tggagcactg
    13861 cccccaacgt tatcgttttg gaccacatga gcgagctcat g
//
LOCUS       DASSFP010000027         5180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1643825, whole genome shotgun sequence.
ACCESSION   DASSFP010000027 DASSFP010000000
VERSION     DASSFP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643825"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFH91_00650"
     CDS             complement(<1..572)
                     /locus_tag="VFH91_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6861512.1"
                     /translation="MRLPLTLFRKVVATVTIVLFLSLSSIAQQNRGSLRGLITDELGA
                     AIVGANVILTSETGVEKRTTTNAEGVYTLNGLAPGKYILQATAAGFAPSLGRELVVNA
                     DRQTLDVTLRVTIEEKVTVATDSSLTTEATDNANQTIVSGKDLDALPDDPDELAAALQ
                     ALAGPSIGPSGGQIFIDGFSGGNLPSKDSIR"
     gene            complement(669..1871)
                     /locus_tag="VFH91_00655"
     CDS             complement(669..1871)
                     /locus_tag="VFH91_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861513.1"
                     /translation="MRALFLAVIVLFTASQISANDLVIQGATQANDPGILAKGAIGEV
                     KAIDSAAKQITLKTDAGSVVLVTFSDKTSYKRLAPGETSLTSATDITLAEVAEGDRIW
                     ARGNVSEDRKSVPATMIVLMTKGDIAKKQEAERLEWRRRGILGVITAIKPDAHEIVIA
                     NRTVSGTQSVVLPVGDKTEMRRYAPDSIKFSDARPSTFSDLKVGDQLRALGDRGEDPL
                     RFTPQKIVTGSFRTVGGVVTAVNAETGEIKINELEKKQPLTIVIKQDAVLRKFPSEMG
                     MMGGGFNRGAGGPGGAPPASGQNAPAAGQGRPQGGPSGPRPGFNINDMLERLPTISIA
                     DVKVGDTIIVSSTQGVDPSRLTAISLVSGADHLLAMLAPRPQPGQATPNPAAGLGGSG
                     ISFGIGLP"
     gene            complement(2084..3082)
                     /locus_tag="VFH91_00660"
     CDS             complement(2084..3082)
                     /locus_tag="VFH91_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6861514.1"
                     /translation="MITCRANTALTPSIHTFRSVNSTRLGALVVCLLMLVGAAYAQES
                     VPQEHNTDAVVITGVSNTDAYGLGQTVRIQGTVKQGAMSFGGDVIVEGVVEGDVATIG
                     GSVIQLPGARIGGDVIVIGGTYYPSDAQPLRKPGATTIMYAGYQQELRDLMRNPLGLI
                     TPRWTPGYMGTRVLVILFWFLVSLGFTAAMPQTISRGIARLKLTSLRVALIGLIGVIV
                     FGAGPILCLYILPQPISVLIGLIALLLWLISGLFGRVILYAATGRWLQSKYFPIARHS
                     EAVALLLGTSFWVLLTSLPFVWPFIAAVILVLSLGLTLTSRYRNSWQRGTQSSPVF"
     gene            complement(3102..3689)
                     /locus_tag="VFH91_00665"
     CDS             complement(3102..3689)
                     /locus_tag="VFH91_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6861515.1"
                     /translation="MVAALKIQNDLCRPADIAAYVDGELNAIAQLSLETHLEDCQQCR
                     DELRNHRRLICELDAALDKSSEMQMPEGFSKLVAARASSDMSGVRSATENRRALAICL
                     ILGIAGFAMVGVTLPRAGFVKLRNLAEGFKALFSVLGSAIYDSLASASVIVRVVSRKF
                     LFETKATAVAVVVVAAAVFILSRLIVNYHRSSATE"
     gene            complement(3689..4309)
                     /locus_tag="VFH91_00670"
     CDS             complement(3689..4309)
                     /locus_tag="VFH91_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6861516.1"
                     /translation="MNIIASSPSKSLTRDFTLVNDCELVATAITGTEGSFEELVRRYQ
                     RPISAYVYRMIGDYESALDLTQEIFIKVYGSLRRYRAEFKFSTWIYKIAHNCAVDHLR
                     RSSTREQSLISGTESDNFELPIESSRLSPEQESERKERRIEIEGVIRALPSNYRELII
                     LRHSQDLTYEEIVEVTGLPLGTVKNRLFRARDMMRQLFVEKGITGI"
     gene            complement(4376..>5180)
                     /locus_tag="VFH91_00675"
     CDS             complement(4376..>5180)
                     /locus_tag="VFH91_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861517.1"
                     /translation="ADAIIRPSFDEPNIGHPDQLAEVLKELATSAGLLRQKRWSLSLP
                     ANTARTLVLTLESQAQSASELQDILKWKMERGFGTTLDELSISRERLQKDAQGRDRYL
                     AVAVNRSILAEYESVINSINWRVGLVLPRQLGESRWLMRNGGLAGDSLLVSGAEHGFT
                     AVFLRDKHPLIVRTVTCNDDECEDEFYRLLLYYRDRNSATDDQGSQLSRMMVIGQSLP
                     KERAGLIVRETMGVDLTPLEAEDLGLTLPGRDLSFDVVAAPAGLATMSL"
BASE COUNT         1301 a         1377 c         1285 g         1217 t
ORIGIN      
        1 cggatcgagt ctttcgaagg caaattgccg cccgaaaaac cgtcaataaa aatctgaccg
       61 ccacttggtc caatcgaagg accagccagc gcttgtaacg ctgccgccag ctcatccgga
      121 tcatccggta acgcatcgag atcctttcct gaaacaattg tttggttggc gttatcggta
      181 gcttctgtag tcagggaact atctgtagcg actgttacct tttcttcgat tgtgacacgg
      241 agcgtaacat ccaatgtctg tcgatccgcg ttcacgacta gctcacgtcc cagcgagggg
      301 gcgaaaccag ctgcggttgc ctgcaggatg tactttccgg gtgccagccc gtttaaggtg
      361 tacacgccct cagcattggt ggttgtccgt ttttcaacgc cggtttcact tgtcagaatt
      421 acgttggccc cgacaatcgc ggctccgagt tcatcggtga tgaggccacg caggctgccc
      481 cggttttgtt gggcaatcga agaaagagat aggaagagaa cgatcgttac agtggcaacg
      541 acctttcgaa aaagagtcag gggaagtctc attgtatcgc ctctggaata cgacatcggc
      601 taacagccgt ttgagtcgag caccgtgaga cgacagaaaa gacttccggt gactctaagc
      661 attaaagctt acggcaagcc gataccgaag gagattccgc tgccgcccag gcctgcagca
      721 ggatttggtg ttgcctgacc gggttgtggt cttggcgcca acatagccag gagatggtct
      781 gcgcctgaga cgagcgatat agctgtcagc cggcttggat caactccttg cgtactggaa
      841 acgatgatag tgtcgcctac cttaacatcc gcgattgaaa tggtcggaag gcgttcgagc
      901 atgtcattga tattgaagcc tggacgagga ccggatggtc caccctgagg tcgtccctgg
      961 ccggcagctg gagcattctg ccccgaagct ggcggcgctc cgccaggacc gcccgctcca
     1021 cgattgaatc ctcctcccat cattcccatc tcggagggaa actttcgcag gactgcatcc
     1081 tgtttgatta cgatggtgag tggttgtttc ttttccaact cgttgatctt gatttcacca
     1141 gtctccgcat tgacggcagt aacaacgcca ccaacggtgc ggaaacttcc ggtcactatc
     1201 ttttgaggtg tgaagcgcaa tggatcttca ccacggtcgc caagtgcacg gagttggtcg
     1261 ccgactttca agtcgctaaa tgtgctcggc ctggcatcgc tgaacttgat cgaatcgggc
     1321 gcgtaacgtc tcatttctgt tttgtcgccc accggaagaa caacagactg cgttcctgag
     1381 accgtgcgat tcgcgatgac aatttcgtga gcgtcgggtt tgattgcagt aataacaccg
     1441 agtattccac gccggcgcca ttccaatcgt tcagcttcct gtttcttggc gatgtcgcct
     1501 ttggtcatta ggacgatcat cgtggccggc actgattttc gatcctccga aacgtttccc
     1561 cgggcccata tccggtctcc ctccgcaact tcagccaacg taatatccgt tgcactcgtt
     1621 aaggatgttt cgcctggtgc caaacgcttg tatgaagtct tgtcactaaa cgtgaccagc
     1681 acgaccgaac ctgcatcggt ttttagggtt atctgcttcg ccgcggagtc gatggcttta
     1741 acttcgccga ttgctccctt ggcgagaatc ccagggtcgt tagcctgagt agctccctga
     1801 ataactaaat cattggctga gatctgactt gcggtaaata aaacgataac tgctaaaaag
     1861 agcgccctca tttagctgtc ctttccgtcc attaggtgga acaccgccac aagcgggttt
     1921 atgacaatgt agctaacaaa gggatttcaa agccgccgaa actctacggt gttacgcctt
     1981 aaactcagta aaggttctgc ggaaatccct cacactcgat aaataaattg taagcgtggt
     2041 gatggtaacg aaccaaatgt gggacacccg gattgtttgt tattcagaac actggactac
     2101 tttgggtccc gcgttgccat gaattgcgat aacgcgatgt caatgtcagg cccaagctga
     2161 ggactagaat tacagcagca atgaacggcc agacaaaagg caaggaagtt aataagaccc
     2221 agaagctcgt ccccagcaat agggccactg cttcggagtg gcgggcgatg ggaaagtact
     2281 tgctctgtaa ccaacgaccg gtagctgcgt ataagatcac ccggccaaac agacccgata
     2341 tcaaccagag aagcagggca atgagcccaa tcaacacgct aattggttga ggaaggatgt
     2401 aaaggcacaa aattggacca gcgccaaaaa ctatcactcc aatcaaacca atcaacgcga
     2461 cgcgaagact tgtgagtttc aggcgagcga tgccacgact gatggtctgt ggcatcgcag
     2521 cagtaaagcc taacgacacc aggaaccaga acaagataac cagaactctt gtacccatat
     2581 aaccgggcgt ccaacgaggt gtaataagac cgagaggatt ccgcatcaag tcgcgtaatt
     2641 cttgttgata acccgcgtac atgatggttg tcgcaccggg tttcctcaaa ggttgagcat
     2701 cgctcggata ataagtgcca ccaatcacaa tcacatcgcc accaatccga gcacctggca
     2761 attgaattac cgaaccacct atcgtggcaa catcgccttc gactacgccc tcgacgatca
     2821 catctccacc aaacgacatt gccccttgct taaccgtccc ctggattcgt accgtttgtc
     2881 ccaggccata agcatccgta ttggacacac ccgtgatgac gaccgcatcg gtattatgtt
     2941 cttgaggaac agactcctgg gcgtaagctg ccccaaccaa catcaggaga cagacaacta
     3001 aggctcctaa tcgagtggag ttaacagagc ggaatgtgtg aatgctgggg gttaatgctg
     3061 tatttgcgcg acaagtaatc atttgccaaa ccggcgagct cttactcggt agcgctcgag
     3121 cgatgataat taacaatcaa acgggacagg ataaagactg cagctgctac aacaacgaca
     3181 gcaacggccg tggctttcgt ctcaaaaaga aatttgcgac taactaccct cacgatcact
     3241 gaggcactcg ctaagctgtc gtaaatggca ctccccagga cactgaacag cgctttaaag
     3301 ccttcggcaa gatttcgcaa ctttacaaat cctgctcgtg gcagcgtgac tccaaccatg
     3361 gcaaaaccag cgatgccgag gatcaaacag atagccagag ctctacgatt ttcagtcgca
     3421 gaacgaacgc cactcatatc actcgaagca cgagccgcga cgagttttga gaatccctcg
     3481 ggcatctgca tttccgacga cttatcaagt gcggcatcaa gttcacagat taaacggcga
     3541 tggtttcgca attcatcgcg acactgctgg caatcttcca agtgggtttc aagcgacagc
     3601 tgtgcaatag cattcaactc gccgtcgaca taggcggcaa tgtcggcagg tctgcagagg
     3661 tcgttctgaa tttttagtgc tgcgaccatt aaatgccggt gattcccttc tcaacgaata
     3721 gctgacgcat catgtctcgt gcacggaaca agcgattctt aacggttccc agaggtaagc
     3781 ctgtcacctc aacgatttct tcataggtca gatcttgtga gtgtctgaga ataatcagtt
     3841 cgcgatagtt agagggcagc gctcgaatta ccccttcaat ttcgatgcga cgttcttttc
     3901 gttcactttc ctgctcggga ctcaaacgcg agctctcaat ggggagctca aaattatcgc
     3961 tttcagttcc gctgattaag gactgctcgc gagtggagct tcgtctaagg tggtcgacgg
     4021 cacagttatg cgcaattttg taaatccagg ttgaaaattt gaattctgcg cgataacgcc
     4081 ggagggagcc gtaaacctta atgaagattt cctgagtcag gtcaagagcg gattcatagt
     4141 cgcctatcat ccgatagacg taggctgaaa ttggacgttg gtagcgcctg actaactcct
     4201 cgaagctgcc ttcagtcccg gttatcgccg tcgccaccag ttcgcagtca tttacgaggg
     4261 taaaatctct ggttaatgac ttggacggcg aagacgcaat aatgttcatg atattgaggt
     4321 tttacgcgcc gtagaagctt tttgtttcac ctaaagggtt tgaaaaaaca gaggttcaaa
     4381 gagacattgt tgccaggccg gcgggcgccg caaccacgtc aaaactgaga tctcggccgg
     4441 ggagtgtcaa tccaagatct tccgcttcga gtggtgtgag gtcaactccc atcgtttctc
     4501 gaacgatcaa gccggcacgc tctttaggca ggctctgccc aatcaccatc attctggaaa
     4561 gctgggaacc ctggtcatca gtcgcggagt tacgatccct gtaatacagc agcaatcggt
     4621 agaattcatc ctcacattcg tcatcattac aggtgacagt gcgaacaatc agaggatgct
     4681 tatcgcgaag aaagactgct gtgaacccat gctcagcgcc gctaactaac agagagtctc
     4741 ctgctaagcc accattgcgc atcaaccagc gcgactcgcc caactgtcgg ggcaacacca
     4801 gtcccactcg ccagttgatt gagtttatga ctgactcata ttcggccagt atcgaacgat
     4861 tgaccgcaac cgcaaggtac cggtcacgac cctgcgcatc cttctgcaga cgttctcttg
     4921 agatcgacaa ttcgtcgagc gtagttccga acccgcgctc cattttccac ttcagaatgt
     4981 cctgaagttc agaggccgat tgagcctggc tttccagcgt taagactaac gttcgggcag
     5041 tattcgcagg caaactcaac gaccaacgct tttgacgaag caatcccgcg ctggtggcca
     5101 attcttttaa gacttccgca agttgatcgg gatgaccaat atttggttca tcaaagctcg
     5161 gacgaattat tgcgtcagca
//
LOCUS       DASSFP010000028         4907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1048345, whole genome shotgun sequence.
ACCESSION   DASSFP010000028 DASSFP010000000
VERSION     DASSFP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048345"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..705
                     /locus_tag="VFH91_00680"
     CDS             64..705
                     /locus_tag="VFH91_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861518.1"
                     /translation="MRPIPQKILFLFICLAIFGLGSLTIQAQSGRRQNKTPELAPVPT
                     PTPEPTPQAKSKPKDSDLGFFLGVDRSTTYMNFYGADYDAIIRGCGDKLRDGSSGFVD
                     VASQDLNRSDAIKKAKAEKTTYVVLLSLQTSTMSSNSNNDIEVEYVVFAPVTGKIVTS
                     GRTYPGSRRAGPVIGPSSTSGLYRDQLLRMAGEDAGERILKSLHLSSGTVIPN"
     gene            complement(718..2598)
                     /locus_tag="VFH91_00685"
     CDS             complement(718..2598)
                     /locus_tag="VFH91_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6861519.1"
                     /translation="MNRAISDIEALPSVAHLNTVSTTDQPTTLVEVFERVARQHKRKD
                     TLNYKLEGRWVSVSSDEMMLRAQRIAAGLYSIGARRGDRVAILSDSRVEWTLTDAGCI
                     FAGVVDVPIYPTLTPPQVKYILNDSGAHVLFVANGEKYDELRGVIAECPEVRQVVLFE
                     GRDAGDATLTLFQLESKGAALLDQQTDLIDHASHEISSADLATIIYTSGTTGEPKGVM
                     LTHSNLVTNLIDSSCHLAFGEKDIALSVLPLSHVFERQAMYMYLNQGMAVYFAESLQT
                     IGPNLREVRPTVLVGVPRIFEKIYDRIQERAAESGRASSLILNWAVGVARSYAGYVIA
                     HKPIPATLKLKHDLASKLVFTKWRDAFGGRIRLLLSGGAALPEELAYIYIGAGIPIIQ
                     GYGLTETSPVITASIIEDNRPWTVGKPIPNVEIRIAEDGEIETRGPNVMKGYYNKPEE
                     TRAVFTSDGWFKTGDIGAIDGEGFLRITDRKKELFKTSGGKYISPQPIEQAIKGSRFV
                     SQVVLIGAGRKFPAALIVPVWEQLESYCKLKGIEVQSRKELCHHPRILDLLHRQIESL
                     TPNLARYEKIKAIALLENEFSIEGGELTPTLKIKRRIIDEKYRDVIDRLYEEAEASRN
                     AE"
     gene            complement(2595..3209)
                     /locus_tag="VFH91_00690"
     CDS             complement(2595..3209)
                     /locus_tag="VFH91_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6861520.1"
                     /translation="MRSARTLTPVRPAIGDKREAILRAAIGVFAHNGYFNSKVADIAK
                     EAGVADGTVYLYFKSKEEILHSIFDRSVEEAIADARKQLAVISDPKEKLRRIALLHLE
                     RLGSDRDLAVVFQVELRGSTKFMEEFSKAGFAEYLALIRSTIEEGQQAGIFRSELNAK
                     VVSKILFGALDEMATNWILSKRRYKLAPMADQVLDIFLNGVTRA"
     gene            3577..4218
                     /locus_tag="VFH91_00695"
     CDS             3577..4218
                     /locus_tag="VFH91_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6861521.1"
                     /translation="MKSSITTILFDWDGTLVDSAHLGLQAFQKAFAELGEPFPLDVYE
                     STYSPNWYATYEALGLPRDKWQRADELWLVHYGDQLAELIDGVGDTLLRLQGAGYKLG
                     VVSSGSNVRVCREIESSRLANVFSVVICNDHVTNKKPHPEGLEKALDCLASRSDEAAY
                     VGDAPEDIQMGKAASVLTVGVRSSYPSSSRLLNAEPDVYLERLSDLERYLSSR"
     gene            4215..>4907
                     /locus_tag="VFH91_00700"
     CDS             4215..>4907
                     /locus_tag="VFH91_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET6861522.1"
                     /translation="MKPNYKTRVKICCIASIEEAWMAIDSGADAIGLVSAMPSGPGPI
                     AEELIAEIVRSVPASIGTFLLTCKQDISSIIAQQRFVKVNTIQICDRLIEGSYDDLRN
                     ALPGVKLVQVIHVTGPESVQEAIEVAPLVDSILLDSGNQSLAIKELGGTGRVHDWRLS
                     RKIRESIDVPIFLAGGLKPENVAEAIEEVRPFGVDVCSSLRPESNLDLGLLASFFSAI
                     EGVSPARTETMIN"
BASE COUNT         1179 a         1232 c         1221 g         1275 t
ORIGIN      
        1 caattttgag atccgtaaat ctcattctcc ccagtttgaa gtcatgcgct ctggagctcc
       61 ataatgagac caattcccca gaaaatttta tttcttttta tttgtctcgc aatctttggg
      121 ctgggttcgc tcacgattca agctcagtct ggacgccggc aaaataagac tccagaacta
      181 gccccagttc ccactcctac tcccgaacca actccccagg ccaaatccaa accaaaagat
      241 tctgacttag ggttcttcct gggagtcgat cgcagcacga cttatatgaa cttctacggc
      301 gcggattacg acgcaataat tcgcgggtgc ggcgataaac tgcgggacgg atcttctggc
      361 ttcgtcgatg ttgccagcca ggatttgaac cggagcgacg cgatcaaaaa agcgaaagcg
      421 gaaaaaacta cttatgtagt gctgctttca ttgcagacgt ccacaatgag ttcaaacagc
      481 aacaacgaca ttgaggttga gtacgtcgtc tttgctccgg tcaccggaaa aattgtcacc
      541 agcggtcgaa cttatcctgg ttctcgcaga gctggtccag tgatcggccc ttcttccacg
      601 tcaggtctat accgcgacca acttttgcga atggcaggtg aagatgcagg agagcgaata
      661 ctcaagtcct tgcacctctc atcgggtaca gtaattccga actgactgct tgaccactca
      721 ttcagcattt cgggaggctt ctgcctcttc gtaaaggcgg tctataacat ctcgatactt
      781 ctcatcgatt attcgacgtt tgatcttgag ggttggcgtt aattcgccgc cctcgatcga
      841 gaattcattt tccaggaggg cgatggcttt aattttctcg taccgtgcga gattaggtgt
      901 cagcgattcg atttgacgat gcagcagatc gagtatccgg ggatgatgac agagctcctt
      961 tcgactctgt acttcaatac cttttaactt gcagtacgac tcgagctgtt cccaaaccgg
     1021 cacgattaga gccgccggaa actttcgtcc cgccccaatt aggaccacct gactgacaaa
     1081 tcttgatccc ttgattgcct gttcaattgg ttgtggcgag atatactttc cgcctgaagt
     1141 cttgaacaac tcttttttgc ggtcggtgat tctcaaaaat ccttcgccat ctatagcgcc
     1201 gatatcaccc gttttgaacc acccatcact cgtaaaaaca gctcgagttt cttcgggttt
     1261 attgtaataa cctttcatga cgtttggacc gcgagtttct atctcaccat cttcggcaat
     1321 tctaatctcg acgtttggaa ttggtttgcc gacagtccat ggccggttgt cttcgatgat
     1381 actggctgta ataaccggcg acgtttcagt caatccatag ccctgaatga ttggtattcc
     1441 ggcaccgata tagatgtaag ccaattcttc aggaagcgcc gcgccccctg atagcaacaa
     1501 cctgatccga ccgccgaatg cgtcgcgcca cttcgtgaag acaagtttgg acgcgagatc
     1561 atgcttcagt tttagcgttg caggtattgg tttgtgagca atgacgtatc ctgcataact
     1621 gcgcgcaaca cctacggccc agttcaagat cagagagctg gcgcgacctg attcagcggc
     1681 ccgctcttga atccggtcat agattttttc aaatattcgt ggtacgccga caagtactgt
     1741 cggtctgact tctcgaagat ttggtccaat cgtttgtaat gattccgcaa agtagactgc
     1801 catcccctgg ttcaaataca tatacatcgc ctggcgttcg aaaacatgag acagcggcaa
     1861 aactgacaaa gcaatatcct tttcaccgaa agccagatga caagaggaat cgatcagatt
     1921 tgtcaccagg tttgagtgag tgagcatcac tcctttcggt tcgccggtcg ttcctgatgt
     1981 gtagattatt gttgccaggt cagcactcga aatctcgtga gacgcgtggt cgattaagtc
     2041 ggtctgctgg tctagtaatg ccgctccttt agactcaagt tggaagagcg tcagagtcgc
     2101 gtctccagca tcacgacctt caaaaagcac gacctgtcta acttcggggc actcggcaat
     2161 aacgcctcgt agctcgtcgt atttttcgcc gttcgcgaca aaaagcacgt gtgctcccga
     2221 gtcgttgaga atgtatttga cttgaggagg agtcagagtt ggatagatcg gaacatctac
     2281 cacacccgca aagatgcatc cggcgtcggt gagggtccac tccactctac tgtctgacag
     2341 tattgcaaca cggtcaccgc gtctggcgcc gattgagtag agtccggctg caatccgctg
     2401 tgctcggagc atcatctcgt cggatgagac ggaaacccag cgcccttcaa gtttgtaatt
     2461 gagcgtatct ttccgtttgt gctggcgggc cacgcgctca aaaacttcaa cgagcgtggt
     2521 cggttggtcc gtggttgaaa cggtgtttag gtgagcgacg gatggtagcg cctctatgtc
     2581 actgatggcc cgattcacgc tcgcgttacc ccgttaagaa aaatgtccag gacctggtca
     2641 gccattggcg ctaacttgta ccgtcgcttg gaaaggatcc aattcgtcgc catttcgtcg
     2701 agtgcaccaa agagaatctt tgagacgact ttcgcgttga gttctgaacg aaaaattcct
     2761 gcttgctgtc cctcttcgat tgttgatcga atcaacgcaa ggtactcggc gaatccggcc
     2821 ttagagaact cctccataaa ctttgtcgag ccgcgaagct cgacttgaaa tacaacggca
     2881 agatcccgat cggaaccaag cctctcaaga tgaagcagtg cgatgcgacg caatttctcc
     2941 ttgggatctg agatcacggc gagctgttta cgcgcatccg cgatggcttc ctcgacactg
     3001 cgatcaaaga ttgagtggag gatttcttcc ttactcttga agtaaaggta gaccgttcca
     3061 tcggcgacac cggcttcttt ggcgatatcg gcaaccttgg aattgaagta accattgtgc
     3121 gcaaagaccc cgatggcagc acggagaatc gcctctcgtt tgtcgccaat tgcgggtcga
     3181 actggagtta acgtacgtgc gctacgcatt tttaatttga gagcagccgt tgagagttac
     3241 cagtcatctg tttttgtgtc gaaataagat tcaaatttta aaatgaatga cgcctcactc
     3301 agtgccttct agcctagtag ctcacgaacg ttttggcaac atgaagttag cagtatgaga
     3361 atttcactgc ccgtgtaaaa aatcaagaaa ccaccggcct cagccgcgtg gtgattcagt
     3421 tttcgcgtag cccggaaaca tctcggtaga tgtcactcct agggagtgaa agcctgccaa
     3481 tcaatcaata catccaagtt gccgcttatg ttagtcaagt ttgccaggac ccgatcgttg
     3541 cgcaacctgg ggagactgct ctttaatatt ccacaaatga aatcttctat caccactatt
     3601 ctttttgatt gggacggaac actcgtcgat tctgctcatc taggcctgca ggcctttcaa
     3661 aaagcattcg ctgagctggg cgaacctttt ccgctcgacg tgtacgaatc aacctactca
     3721 cccaattggt acgccactta cgaagcactt ggcctaccgc gcgataagtg gcagcgcgct
     3781 gatgagttgt ggttggtcca ttatggtgat caacttgcag aattgattga tggggtcggc
     3841 gatacgttac tgcgattgca aggcgccgga tacaagctcg gggtcgtttc aagtggatcc
     3901 aacgttcgcg tgtgtcggga aatcgaatcg tcccggctgg caaatgtgtt cagcgtcgtt
     3961 atctgtaatg atcacgtgac caacaagaag ccacatccag agggattgga aaaggcgctc
     4021 gactgtctgg cgagccgtag cgatgaagct gcctatgtgg gcgatgctcc ggaagatatt
     4081 cagatgggga aagctgccag tgtccttacc gttggtgtca ggagctctta tccgagcagc
     4141 tcgcggctct tgaacgctga accggatgtc tatctcgaga gactttcaga ccttgaacgt
     4201 tatctgagta gccgatgaaa cccaattata aaacaagagt caaaatctgc tgcatagcga
     4261 gtatcgaaga agcgtggatg gcaattgatt ctggcgcgga cgccatcggc ctggtatctg
     4321 ctatgccaag tggccctggt ccaattgcag aagaactgat agctgagatc gttcgttccg
     4381 ttccagcgtc tatcggcaca ttcttactaa cttgcaagca ggatatttct tcgatcatag
     4441 cgcaacaacg gttcgtaaag gtgaacacaa ttcagatctg cgatcgcctg attgaaggaa
     4501 gctacgacga tctgcggaat gctttgcctg gtgtcaaact ggtccaggta attcacgtta
     4561 ctggtcccga gtcggttcag gaagctattg aagtagcccc actggttgat tcgattctgt
     4621 tggattccgg aaatcagtcg cttgcgatta aagaattagg tgggacaggc cgcgtacacg
     4681 attggagatt gagtcgtaaa attcgagaat cgatcgatgt tccgatattt cttgcgggag
     4741 gcctcaagcc ggagaatgtc gcggaggcga ttgaagaggt cagaccgttt ggtgttgacg
     4801 tttgcagcag cctgcgacca gaaagcaatt tggatcttgg cttgctcgcc tcgttcttct
     4861 cggcgattga aggtgtcagc cccgctcgaa ctgagacaat gatcaac
//
LOCUS       DASSFP010000029         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1942254, whole genome shotgun sequence.
ACCESSION   DASSFP010000029 DASSFP010000000
VERSION     DASSFP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942254"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            664..2688
                     /locus_tag="VFH91_00705"
     CDS             664..2688
                     /locus_tag="VFH91_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6861523.1"
                     /translation="MNAINVASLINLLGYTIGIALYAILLAIVLKHPIQAGSPSNYNV
                     TAPSGRLAVNGLMLATAILGMLWNTGALATYGLRGFGLFANEAHPLLSAIAFTALGFL
                     PAVVVQSVLLGRLNQTEARVSRAISIAAYCLSSIAGALHFYTAMTAGSSPSLWALRLL
                     TLGYVALLAALFLATRRQEGWKKAAWAAALAVFAVSALHLSHHRGSADAWYTELLGHH
                     ASLPLAFAILYEDYRFAFADIFLKRALALLSLVALAFLLYIGIVSPLLASKHVVTQDP
                     RLMGLLLGLWIATAIVYPWLKRVSVIFVDRVMLRRADYTKLRADVSQLLTEHETSEDT
                     LNAVCRLLGPALSAHEISWVTVGDNEPSNDGKRRAVNERPIETINYEFVTLLEGSASR
                     SAIVLIPTAEPPYYQLNIGTLSGGRRLLSDDIAMLDSVALIVARSIDSLRVTHERCEQ
                     SLREQEINKLATEAQLRALRAQLNPHFLFNALTTIGYLVQTAPDRALDTLMKLTSLLR
                     AVLRTEGEFVTLGEELELIDSYLDIERARFEERLRATIDVPDELRSIRVPSLLVQPLV
                     ENAIKHGIAPSRVGGDLTISARLQHHPDGGLLTIIVSDTGLGADKIDWTQGRRTGVGL
                     SNLEARLRSYGGAAASLQIHSTRGTGTMVEMRIPIQKPKAIQVEANRSVA"
     gene            2705..3487
                     /locus_tag="VFH91_00710"
     CDS             2705..3487
                     /locus_tag="VFH91_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET6861524.1"
                     /translation="MTVKLRIVIADDERPARSFLAAILRTFDDVEIVGEARNGSEAVE
                     IIERERPDLALLDLQMPEIDGLGVVRLLRKSTTPLIGFVTAYDEYAVRAFEVNAVDYL
                     LKPVDRARLRETLNRAQERLEREDLRGELVSHLHAAAADYEAATRPVFLERMPVRERN
                     EIILLPVRQIASIVADGELLQISTINNEHHSINYRLKDLEARLDPALFVRIARGTIVN
                     IETIVRVSPMPGGTFVITLKNNQQLQASRLQSRILRDGLLRL"
BASE COUNT          882 a          886 c          924 g          902 t
ORIGIN      
        1 tatgaatagg ttggaaacaa gaacaagaca aattaacgaa cggaaaaggc gagtaggcat
       61 aatttaacac ccttatgctt gagtccttca cgaaatgcgt ttgccgactg cctgcgccag
      121 tagggttcgg tttgacgtca acccccgtgg ttcaaggacg aataaagcaa acattgggga
      181 ccgattaatc gtagtgttgg aaggtgggtt gttggatact ggttttcaat gaaacaccta
      241 aagatgaggt tgaatagtct tttggcgtaa acgaaatgat cttagttgag taggtgttaa
      301 tgttttcaac gctggctttg ctggtccttt ttttggcggc gaccgctgcc taagacggac
      361 aagagtccca cctattgtcc gacagactct taggcctgat ccaggcagcg gtcgtctttc
      421 tccggtaggc gtgccggagc gcgcgcaact tatctgggag gtttgagtgt ttgccagaac
      481 ttttcaatga atagtctttc cagcagaatc agtggtcgca tatcccagtc aggtgggact
      541 taaacagaga atcgtccaca gattgcgatg atgacgccga gtttttaaaa cgcggacgag
      601 ttattatgag caattattga actgagagtc tgtgtaaacg ccgtaatctg cggataactt
      661 gtcatgaacg caatcaacgt cgccagctta ataaacctat tgggctacac gataggtata
      721 gctctctatg cgatcttgct cgcgattgtc ttgaagcacc cgattcaagc cggtagccca
      781 tctaactata acgtcacggc gccttccgga agattggccg taaacggatt gatgctcgcc
      841 actgcgatcc tgggcatgct ttggaatact ggcgcattag ctacatatgg gttgaggggc
      901 tttgggttat tcgccaacga ggctcatccg cttttatctg ctattgcctt caccgccctc
      961 ggattcttgc cggcggttgt tgtgcaatcg gtactacttg gcagattgaa tcagaccgaa
     1021 gcccgagtgt cgcgtgcgat ttcaatcgct gcttattgtc ttagctcaat cgccggtgcg
     1081 ttgcatttct acacggctat gacagcgggt tccagtcctt ctctctgggc actacggcta
     1141 ctcacattgg gttacgtcgc actattagcc gcactttttc ttgcgacgcg acgacaggaa
     1201 ggctggaaaa aagcagcctg ggccgccgca ctagcggtct tcgcagtttc ggcccttcac
     1261 ctgagtcatc atcggggcag tgcagacgcg tggtacacgg agcttctggg ccatcacgct
     1321 tcccttccat tggcgttcgc aatcctctac gaggactatc gctttgcttt tgccgacatc
     1381 tttctaaagc gggctcttgc cctcctgtca ctcgttgcat tggcattcct gctttacatc
     1441 gggattgttt cgcctttgct ggctagtaaa catgtggtca cccaggaccc gcgtctaatg
     1501 ggtttgttac tgggcttgtg gatagcgact gccatcgttt acccatggct caagcgcgtg
     1561 tccgttatct ttgtggatcg cgttatgctc agacgcgctg actacacaaa attacgcgcc
     1621 gacgtgagcc aacttttaac cgaacatgaa acttctgaag acacgctcaa tgcagtgtgt
     1681 cgtctacttg gaccagcact ctcagcacac gaaatcagct gggttacggt cggcgacaat
     1741 gaaccttcga acgacggcaa aagacgagcg gttaatgaac gtcctatcga aactatcaat
     1801 tacgaattcg tgacattgct tgaaggatct gcaagcagga gtgccattgt tttaatacca
     1861 acagctgagc caccctatta tcaactcaac atcggcacgc tttcaggtgg acgaagactg
     1921 ctttcagacg acatcgcgat gctcgattcg gtggcactca tcgttgcgag aagtatcgat
     1981 tctttgagag taacgcatga acgttgcgaa cagagtttgc gagagcagga gatcaacaag
     2041 ttagcaactg aggcccaatt gcgtgcgctg cgtgcccagt tgaaccctca cttcctattc
     2101 aatgcgctta caaccatcgg ctacctggtc caaaccgcgc cggatcgggc gctggacaca
     2161 ctgatgaaac ttacaagtct gctcagagct gtcttgcgca cagagggaga gttcgtaacg
     2221 cttggtgaag aactagagct gatcgattcg tatctggaca tagagcgagc cagattcgaa
     2281 gaaaggctgc gagctacaat tgatgtacca gacgaattac gttcaatcag agtgccttcg
     2341 ttgttggtcc aacctctcgt ggaaaatgcc attaagcacg gtatcgcccc atctcgtgtc
     2401 ggcggagatc tcactattag tgcgcgactg caacaccacc ctgatggcgg attacttacc
     2461 atcatcgtca gtgacactgg cctcggtgcc gacaaaatcg attggactca aggccggcgt
     2521 accggagtcg gtctgtcaaa cctcgaagcg cgtcttcgtt cctatggtgg cgcagcggca
     2581 tcattgcaaa tccatagcac tcgaggaact ggaacgatgg ttgaaatgag aataccaata
     2641 caaaaaccga aagccattca agttgaagcc aataggtccg tggcatgact gagacgttga
     2701 cgacgtgact gtaaagcttc gaatagtaat tgcagatgac gagcgtccag cgcgctcttt
     2761 tcttgctgct atcctgcgaa cgttcgacga tgttgaaata gtcggagaag cacgaaacgg
     2821 gagcgaggct gtcgaaatca tcgagcgaga aagaccggat ctggcattac tcgatttgca
     2881 aatgccggag attgatggtt tgggcgtagt acgactactg cgtaagagta cgactccgct
     2941 cataggattt gtcaccgcat atgacgaata cgcagtgcgt gctttcgaag ttaatgccgt
     3001 tgattattta ctgaaaccag ttgatagagc gcgccttcgc gaaactctga atcgggccca
     3061 ggaacggttg gaaagagaag atctacgagg tgaactcgtg tcccacttgc acgcagcagc
     3121 tgcagactat gaggcggcaa ctcgtcccgt ctttcttgag cgcatgcctg ttcgtgagcg
     3181 caacgagatc attctcctgc cagtaagaca gattgcatcg atcgtcgccg acggtgagtt
     3241 gcttcagatt agcaccataa ataatgaaca tcactcgatc aactatcggc tgaaggacct
     3301 tgaagcgcgg ttagatcctg cactattcgt tcgaatagca cgggggacga tcgtcaacat
     3361 cgagacaatt gttcgcgtaa gtcctatgcc gggcggcaca tttgtaatca ctctcaaaaa
     3421 caatcaacag ctacaagcga gtcgcctaca gtcgcgcata ttacgtgacg ggttgttaag
     3481 actctgagcg ttgtactgaa tccaagtgtt ctataaatca ttcggtgagg tcttttcgca
     3541 gtccagcgtt ttgataagag atgcgcgcaa tagaagccca atcggcttca ccca
//
LOCUS       DASSFP010000030         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_6594, whole genome shotgun sequence.
ACCESSION   DASSFP010000030 DASSFP010000000
VERSION     DASSFP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6594"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VFH91_00715"
     CDS             complement(<1..1246)
                     /locus_tag="VFH91_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6861525.1"
                     /translation="MKFRQILILTVFSFIAATSPSAAVTAAPTQTPPPAAQSEIECGC
                     EDNPSLDLLAIVNGVKIRQVDLSVDTRSKIRLLQDTVIEARSHELDRVIASNLLEAAA
                     KSRGLTARKLLQIEVTGKVVPPTDAEAQEFYKRNLQRMVKDYKDAKAGILATLKSERE
                     VQEAQKFATALRAAADVKILVDKITPPANAEQRLRVIATVNGRPITSGDIEDSLAPLI
                     FQVKTQTYAYRKAELDLKINDLLLEQEAKKQGVAPLALVSREISGKLQMITDQQVLAF
                     YKENKSQFNQEFSKVQLQIVKYLLEQQQEQLANAYADQLRSRAVVQVFLKPPQPPSYR
                     ISVANQPSRGNDDATVTVFEFIDFENPRCAVQFRILEQLIADFGTQVRFVVRDFPEPT
                     HKSSVKAAEAAEAAYQQGKYWEY"
     gene            complement(1246..2214)
                     /gene="hemB"
                     /locus_tag="VFH91_00720"
     CDS             complement(1246..2214)
                     /gene="hemB"
                     /locus_tag="VFH91_00720"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET6861526.1"
                     /translation="MVHITHRPRRLRRSDALRSLVRETRLSADDFVLPLFVCPGTGVR
                     NEVSSMPGVYNLSVDEATKDVSAAFDGGVRSVILFGLPETKDELASGAFAEDGIVQRA
                     IRSIRRTTPEVIIMADTCLCEYTSHGHCGVVRDGEVLNDESLELLARTALSQAEAGAD
                     VVAPSAMMDGQVGAIRETLDDAGLENTAIMAYAVKYASGFYGPFREAANSAPSFGDRR
                     AYQMDPPNAREAMREAELDYSEGADILMVKPATVYLDVLKMVRDRFDVPLAAYHVSGE
                     YALIKAAAQKGWIDEQRVMLETLTSIKRAGADIILTYYAREAIRAL"
     gene            complement(2240..2704)
                     /locus_tag="VFH91_00725"
     CDS             complement(2240..2704)
                     /locus_tag="VFH91_00725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6861527.1"
                     /translation="MTNKSIQETYAPNLACFGCGPANPKGLHIRSYPDGEEVIAEWEP
                     KQEYEAFPGVLSGGIIGTLLDCHCNWAATWHLMKKNGADKPPCTVTADYAITLRRPTP
                     TDGTIKLRARVVESTEDRAVVEGELIAHDKVCATCRGTFVAVKPGHPAYHRW"
     gene            complement(2712..4286)
                     /locus_tag="VFH91_00730"
     CDS             complement(2712..4286)
                     /locus_tag="VFH91_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET6861528.1"
                     /translation="MAFKMNLFEAIFNQRANSCSVIFGGREISYDDLKRSTLKMAESI
                     SQLGVRRGDRIAFLLNDSPEFISAFIATCSLGAIAVPVNMALRPEEQCSILHNSEARL
                     ALIEAEIRKLFLTNAFEKLQDLESVAVIDRKKPHNTAEFEQIRRKQVTRPRETSDQPV
                     REMISPGPDDPAFILYTSGSTGEPKGAVHTQADIFATNDGFCRQVLNLTPTDRLFSSS
                     RLPFAYGLGNSFSFPLLNGATTILCADKPTPEVIRGIFAEHRPTIFFGVPVVYNLLLE
                     HHNNGNNLDCSSLRFCVSAGEALPAHLGETWEKTFGISILDGIGSTEMLHMFMSNHQN
                     DVKYGSSGKVLQGYEAKLLDAEGASVTAANQEGHLWIKGASAARGYWKRPEETAKTFV
                     NGWVRTGDLYLLDQDGYCFHMGRSDDCFKSSGQWVSPVEVEGVLLRHGGVARAAVVED
                     FDDEQLPCACAFIVSQDVESNHSEIEQELRALAAEKLPRFKRPRRYIFLSELPYTATG
                     KIQRFKLRRELREHRS"
     gene            4382..>5271
                     /locus_tag="VFH91_00735"
     CDS             4382..>5271
                     /locus_tag="VFH91_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861529.1"
                     /translation="MIELLMLLGLLLLVFLFIVAFGHGVWLIGESVVRSFNRNSSPFP
                     VSVTKDAELSNGVLGDLLVTERQLSKLHVAGSLDDFTYRHLIELIDIERAKLRTPVPE
                     YSERPDAKSVSETFYVETPQSEEPVPLPTAIQADNEFDASSTITPPSTVQVDDGPSVP
                     LEQTKRELKQKRSFSEMLNSFMEESNIRWGEIIGGLLIIGCSTALVVSLWAQISQIPV
                     LKFLIFTTVTAVLFGIGLYTEHRWKLPTTSRGILTIATLLVPLNFLAIAAVSSGNSAG
                     ALVLASELIAPAIFLCLVYFA"
BASE COUNT         1242 a         1363 c         1284 g         1382 t
ORIGIN      
        1 cgtactccca atattttccc tgctgatatg cggcctcagc ggcttctgct gctttcacgc
       61 tggacttgtg cgttggttcg ggaaagtctc ggactacgaa acgcacttgt gttccaaagt
      121 ctgcgatgag ctgctcgaga attcgaaatt gaacagcaca acggggattc tcaaagtcga
      181 taaactcgaa aacagttacc gtcgcatcat cgtttcctcg actgggctgg ttcgcgacgg
      241 aaatccggta ggacggaggt tgtggcggct tgaggaatac ctggaccacg gcccgacttc
      301 tcaactggtc cgcataggca tttgccaact gttcttgttg ctgctccaga agatatttaa
      361 ctatttgcag ttgcaccttg gaaaactctt gattgaactg cgatttgttc tccttataaa
      421 aggcaagtac ctgttggtcg gttatcattt gtaacttgcc gctaatctct cgactgacga
      481 gcgccagagg agcaacgccc tgttttttcg cctcctgctc caaaagtaga tcgttgatct
      541 tcaggtcgag ctccgcttta cgatacgcat aggtctgagt cttaacctga aaaattagcg
      601 gcgccagact gtcctcaatg tcgcctgaag taatcggcct gccgttaacg gtggcaatga
      661 ctcgcaagcg ttgttcagcg tttgctggtg gtgtaatttt gtcgactaga attttcacat
      721 cagccgctgc ccgcaacgca gtggcaaatt tctgagcttc ttgaacctct cgctcacttt
      781 tcaaggttgc gagtatccct gctttcgcgt ctttgtaatc cttgaccatt ctctgcagat
      841 ttcgtttgta gaactcctgg gcttcggcgt cggtaggagg aacgactttg ccggtaactt
      901 caatttgcag gagtttgcgt gccgttagtc ctcggctctt tgcagcggcc tcaagaaggt
      961 ttgacgcaat tactctatcg agttcatgac tccgagcttc tataacggta tcttgcaata
     1021 gtcggatttt cgatctggta tcaacactca aatcaacttg acgaatcttg acgccgttaa
     1081 cgatagcgag aaggtcgaga gatggattgt cttcgcagcc acattcaatt tctgattgcg
     1141 cggcaggagg cggcgtttga gttggagcgg cagtaacagc cgctgaaggt gaagtcgcgg
     1201 caatgaatga gaacaccgta agaattagta tttgacgaaa cttcatcaaa gcgctcggat
     1261 tgcttcacga gcataataag tgagaatgat atcggctcca gcgcgtttga tactcgtcaa
     1321 agtctccagc atcacgcgtt gttcgtcgat ccatcctttt tgcgcagcgg cctttatcaa
     1381 cgcgtactct cctgagacgt ggtaggccgc aagtggaaca tcgaagcggt ctctgaccat
     1441 tttgagaaca tcgagataaa ccgtcgcagg cttgaccatc aaaatatcag caccttccga
     1501 ataatcgagc tcagcttcgc gcatggcttc gcgcgcattg ggtggatcca tctggtaggc
     1561 ccgacggtca ccgaacgatg gagcactatt cgccgcttca cgaaatggac cataaaaacc
     1621 tgacgcatac ttcacggcat aagccatgat cgcggtgttt tccagtccgg catcgtccag
     1681 agtttcacga atcgcgccga cctgaccgtc catcatcgcc gaaggtgcaa cgacatccgc
     1741 tcccgcttcg gcttgactca gcgcagtgcg tgcaagcaac tcgagcgact cgtcattaag
     1801 tacctcgccg tctcgaacaa ctccacaatg tccatgtgat gtgtactcgc acaaacaggt
     1861 gtcagccatg atgatgactt cgggggtcgt ccgccggatt gacctgatgg ctcgttgtac
     1921 gattccgtct tctgcgaaag ctccggacgc aagttcgtct tttgtttcag gcaaaccaaa
     1981 aagaatgacg cttctaaccc caccgtcaaa agctgcagac acgtctttgg tagcttcatc
     2041 gactgacaaa ttatagacac ccggcatcga tgaaacttcg tttcggactc cagttccggg
     2101 acagacaaac agcggtaaga caaaatcgtc ggcagacaaa cgggtttccc ggacgagcga
     2161 gcgaagggca tcggaacgtc tcagccggcg cggacgatga gttatatgga ccacaagcta
     2221 cctcttgaag ttttactcgt taccaacggt gatatgcagg atgaccgggt ttcacagcca
     2281 caaatgttcc tctgcatgtc gcgcagacct tgtcgtgggc gatcaattcg ccttcaacta
     2341 ccgcacgatc ctctgtcgat tcgaccaccc gtgctctgag cttaatcgtt ccatcagtag
     2401 gtgtcggacg tcgtaaagtg atcgcgtaat cggccgttac agtgcaaggc ggtttatctg
     2461 ctccattctt tttcatcaga tgccaggtag ctgcccaatt acagtggcaa tccaacagcg
     2521 tgccgatgat tccaccacta agcacaccgg gaaacgcctc gtactcttgt tttggttccc
     2581 attcggcgat gacctcttca ccgtcggggt agcttctgat atgcagtcct tttgggtttg
     2641 caggtccaca accaaaacaa gcaaggttgg gagcatatgt ctcctgaata cttttgtttg
     2701 tcatatttgg ttcaggagcg atgctcacgt aattctcgtc ttaacttgaa tctttgaatc
     2761 tttcccgtag ctgtataagg caattccgac aaaaaaatgt accgtcttgg tcgtttaaat
     2821 ctgggcaatt tctcagctgc cagagcacga agttcctgct cgatctctga atggttcgat
     2881 tctacatcct gactgacgat aaaagcacac gcacagggaa gttgttcgtc gtcaaaatct
     2941 tcgacgactg ccgcgcgagc cacgcctcca tgtcgcaata agacaccttc aacttcgact
     3001 ggcgacaccc attgcccact agatttgaaa cagtcgtcgc ttcgacccat gtggaagcag
     3061 tagccatctt gatcgaggag ataaagatcg ccagtcctta cccacccatt tacgaatgtc
     3121 ttcgctgtct cctccggccg tttccagtat cctcgtgcag cgctagcacc ttttatccag
     3181 agatgccctt cctgattcgc ggccgtaacc gaagctccct cagcgtctaa caactttgcc
     3241 tcgtaaccct gaaggacttt gccgctgctg ccatacttaa cgtcattttg atggttcgac
     3301 atgaacatgt gcagcatttc cgtcgagcca atcccatcaa gtatcgaaat gccaaatgtc
     3361 ttttcccaag tctcgccaag atgagccggc aacgcttctc ccgctgagac gcagaagcgc
     3421 aaactggaac agtcgagatt gtttccgttg ttgtgatgtt ctaacaacag attgtagaca
     3481 acgggcactc caaaaaagat cgtgggccta tgttcggcaa agatgccgcg tatgacttcg
     3541 ggcgtcggtt tgtcggcaca gagaatcgtt gtcgcaccgt taagcagcgg gaaagaaaaa
     3601 ctgttgccga ggccataagc aaatggcagc cgagatgatg agaacagtcg atcagttggg
     3661 gtcaggttca acacttgtct gcagaagcca tcattggtgg caaagatgtc tgcttgagtg
     3721 tgaactgcgc ccttaggctc tcctgtgctg cctgaggtgt agaggataaa cgcaggatcg
     3781 tccggccctg gtgaaatcat ctctcggact ggctgatctg aagtctctct gggccgagta
     3841 acttgtttgc ggcgtatctg ctcaaactcg gctgtgttgt gaggcttttt gcggtcaatt
     3901 acggctaccg actcgaggtc ctgcaacttt tcgaacgcat ttgtcaggaa tagtttgcgt
     3961 atctccgcct caatcagggc aagacgtgct tcggagttgt gaaggattga acattgctcc
     4021 tcagggcgca aagccatgtt tacaggtaca gcgattgcac ctagcgaaca ggtggcgata
     4081 aaggcactga taaactctgg cgaatcattt aagagaaacg cgatccggtc gcctcgtctg
     4141 acgcccaatt gcgatattga ctctgccatc ttcagagtac ttctcttaag gtcgtcatag
     4201 cttatttctc tgccgccgaa aatgacggaa caggagttag ctctttggtt aaagattgct
     4261 tcgaacagat tcatcttgaa agccatccac tggtcggtga ttataagcaa ctagacattt
     4321 aaccgagagc cgcttgacgc aggcatctaa aacgagagtc ttctagccgc cgaggttcga
     4381 catgatagaa cttttgatgt tattgggttt gttattactg gtctttttat tcattgtggc
     4441 gtttggacac ggagtttggt tgatagggga gtcagtggtc cgttctttca accggaactc
     4501 gagtccattc ccagtctccg tcaccaaaga cgctgaactg tcgaacggag ttctcggcga
     4561 tctattagta acagaacgac aactctcaaa acttcatgtg gccggctctc ttgacgactt
     4621 cacctatcga catctgatcg agttgataga tatcgagcgc gcaaagctcc gaacaccagt
     4681 tccagaatac tccgagcgac cggatgctaa atccgtatct gaaacgttct acgttgaaac
     4741 tccgcagagt gaggagcctg ttccattacc gacagccatt caggcggaca atgagtttga
     4801 cgcatcatca actattacgc cgccctccac ggttcaagtt gatgatggtc catcagtgcc
     4861 attagagcaa acgaaacggg agctaaaaca aaagcgctcg ttctccgaaa tgctcaactc
     4921 ctttatggag gagagcaata tccgttgggg cgagataatc ggtggactat taatcatagg
     4981 ctgttcgaca gcgttggtag ttagtttgtg ggcccagatc tcgcaaattc cagtcttaaa
     5041 gtttttgatc ttcacgactg tcactgcagt tctattcggt atcggtttgt acactgaaca
     5101 tcgttggaag ttgcccacaa ccagcagagg cattctcacc atcgcgacgc tcctggtccc
     5161 actaaacttt ttggcaattg ccgccgtctc ttccggaaac tctgcaggcg ctttggttct
     5221 cgcaagcgag ttgattgcgc ccgcgatctt tctttgcctg gtctattttg c
//
LOCUS       DASSFP010000031         4367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_303185, whole genome shotgun sequence.
ACCESSION   DASSFP010000031 DASSFP010000000
VERSION     DASSFP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303185"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1302
                     /locus_tag="VFH91_00740"
     CDS             223..1302
                     /locus_tag="VFH91_00740"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET6861530.1"
                     /translation="MTSESAMEPSSSNNSLPPEVRDASAWYGSELVTRTDWIERFSEA
                     EIGEVERVVHEIEKSSVDVATLNTRDTPLPSLGPRLQRLLDEVLNGRGFVLIKGLPVE
                     RWSKREAAIAFLIIGVHLGPLRMQNAEGHLLGHVRDMGRSSTDPNTRIYQTRERQTHH
                     TDSCDVVGLLCLRAARSGGLSNLVSSTTIFNEMRRRRPDLLRVLLDPIETDRRGEVPE
                     GSKPYFTIPVFNYYEGLVSAKYQRQYIESARRFPGVAPLTAEQIEALDLLDQLANDPQ
                     LNLLMELEPGDIQLVHNHTILHDRTAFEDFPEPERKRHLLRLWLAPSGARRLPEIYAE
                     RFGSIRQGDRGGVSVPGARATIPFE"
     gene            complement(1591..3045)
                     /locus_tag="VFH91_00745"
     CDS             complement(1591..3045)
                     /locus_tag="VFH91_00745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6861531.1"
                     /translation="MNPRFIVLLSVILLLTFPVTAQGDQIDAYIKTEMAVQHIPGLSL
                     CVVQHNRIVKDQTYGLANVELNVPATADTEFAIASMTKSVTASAIMLLVQDGKISLED
                     PISKYFDGLPESWQAITIRQLLSHTSGIKDHFRDYPFFPPAKMDRKLEYTDAEFIRAL
                     VDGGLNFKPGAQWAYSGTGFTLLGEIITKVAGKPYSEFFRERIFVPLGMTKTHVISLT
                     DIIPHRAAGYRWLSGTQRNGDYTGQTFSSGADVSLLTTAADLAKWNIALSSEGLWKKS
                     SLEQMWTPANLANGEDAITFPGGEFGLGWFLTSYNGYRLVSHSGSFMTGFTSFMARIP
                     DKDLVVIVLTNQHSANPLQLAFGLLGFYDAGLVAPHQMKVQPDTQPELTAKVKAFMTA
                     LFSGGVKEDPTKFVTPGLAHHLPPIPKPPGGLTAPLSDISFISSDDIRNRSIERYGSR
                     VARMSYYKALFYGDPVHITFYFAADGQVADYSGY"
     gene            complement(3327..>4367)
                     /locus_tag="VFH91_00750"
     CDS             complement(3327..>4367)
                     /locus_tag="VFH91_00750"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HET6861532.1"
                     /translation="VVWLKNGSGLIAPIGEGTQGTQVWFISQPDGAAKRVTNDLNGYG
                     AVSFGVSGDSKTIVTVQSTPSYQIWTSVFGEPTAQSVEITHGEGDGTGGVDWSPDDRI
                     VYFTDTGESRELVSVKLDGTEMRSILKQSEPASDPAVAADGKTIYFMSMRSGTPHIWR
                     INTDGTDLKQITSGDFADFHPTVSPDGKWVLFCSWRSGTQVYWKVPSEGGEAERVTEV
                     RTPLARFSPDGKSIATVQFVKGGTPPWQLAVVTIDGSSATKIIAPPTHFNLRWSSIAW
                     TMDGRAIIVNSDQRGVGNLWSQPIDGSAPKQITNFTSDLISNFAISHDGKRLAISRGN
                     SSLDVVLIKNFH"
BASE COUNT         1030 a         1134 c         1132 g         1071 t
ORIGIN      
        1 ctttcgccta ctggctttca cctgaggaag aaagttctta cgctccaccc gatactcaaa
       61 gatgaataaa tcgtcgggtg agttttgggg gcgcactaat tgtacaccat ggcccggtcc
      121 aacggcctgg aaaagtcgcc atgggtttca tccaaagatt tcgcagattt cgcagattac
      181 tgatcaatct gcggaatcgg tgtaatctgt ggatgaatga aagtgactag tgagtccgcg
      241 atggagccat catcatctaa caactctttg ccacctgagg ttcgcgatgc gtcggcgtgg
      301 tacggttcgg agctggttac acgtacggat tggattgagc gcttctccga agctgagatc
      361 ggcgaggtcg agcgtgtggt ccatgaaata gaaaaatctt cggtcgacgt cgcaacgctc
      421 aatactcgtg acactccact gccctctctt ggaccgcgtc tacaacgtct gttggacgag
      481 gtgctcaatg gacgtgggtt tgttctgata aaaggcctgc cggttgagcg ttggagtaaa
      541 cgtgaggcgg cgatcgcttt tctgatcatc ggcgttcacc ttgggcctct gaggatgcaa
      601 aacgccgaag gacacttgct gggccatgtt agagatatgg gacgatcgag cacagatccc
      661 aacacgcgga tttaccagac acgtgaacgt cagactcacc acaccgattc ctgtgacgtc
      721 gtagggctct tgtgtttgcg agctgcccgg tctggtggcc tgtcaaacct ggtatcctcc
      781 accacgatct tcaacgagat gcgtaggcgc cggccagacc tactccgagt cttactggat
      841 ccaattgaaa cggatcggcg cggcgaagtt cctgaaggca gtaaacctta cttcaccatt
      901 cccgtcttta attattacga aggactggtt tctgcaaaat accagcgcca gtacattgag
      961 tcggcccgac gctttcccgg tgttgcacca ctcacagcag aacagatcga agcactggat
     1021 ttgttggacc aactcgcaaa cgatccgcag ctgaacttgc tgatggaact tgagccgggg
     1081 gatattcagc tcgtccacaa tcatacgatc ctgcacgatc gaacagcgtt tgaggatttt
     1141 cccgagcctg aacggaagcg acacttactg cgtttgtggt tggcgccgtc cggagcgcgt
     1201 cgtctgcctg aaatttatgc ggagcggttt ggatcgatca ggcagggtga ccgcggaggc
     1261 gtatcagtac caggagccag ggccactatt ccgtttgagt gaagtcttct cagcgttctc
     1321 tgcgtaacta ccagtagacc caatcattaa ctagttgcac gctgaaactc cgttcaacgc
     1381 agagttttga atcatcgtcc gcacgccgcg tttaagcaat gccgttggaa tgtccgatat
     1441 gcttcaactt gtcggtcatg aggcacactc aagttcagag gtacgagtca acgacgaccg
     1501 acaagctgaa gcatatcgga cattccgact accagttaat atttggtcta ctcttagtta
     1561 cgctgtggaa aaccgatacg actcaatacc tcaatagccc gaataatcag cgacttgtcc
     1621 gtcagcggcg aaatagaaag ttatgtgaac gggatctcca taaaatagcg ccttgtagta
     1681 actcatccgc gcgacccttg aaccgtaacg ctcgatgctt ctatttctaa tatcatcact
     1741 cgaaataaat gaaatgtcag acagaggcgc tgttagtccg ccgggcggtt tgggaatggg
     1801 cggcagatga tgggccaagc ctggcgtgac gaatttggtg gggtcttctt tcacgcctcc
     1861 gctgaagagt gctgtcataa acgctttcac ttttgcggtc agttccggct gcgtatccgg
     1921 ctgaaccttc atctgatgtg gcgccaccaa acccgcgtcg tagaaaccca acagtccaaa
     1981 cgcaagctgt agtggattcg cagagtgttg attcgtcaac acgatcacta caagatcctt
     2041 atccggaatc cttgccatga aagacgtaaa gccggtcatg aagctgccac tgtgactcac
     2101 gagtcgatac ccattatagc tagttagaaa ccaaccaaga ccaaactcgc cgccgggaaa
     2161 tgtaattgca tcttcaccat tagcaagatt cgctggtgtc cacatctgtt ccaggctcga
     2221 ctttttccac aagccttcag aagaaagagc gatgttccat ttggcgaggt ccgccgcagt
     2281 ggttagcaaa ctgacgtcgg cgccactgct aaaggtttgt ccagtgtagt cgccgttccg
     2341 ctgtgttcca ctaagccagc ggtagccagc agcgcgatgg ggaatgatgt cggtgagact
     2401 aatgacgtgc gtcttcgtca tgccgagcgg cacgaaaatt cgctcgcgga aaaactcaga
     2461 gtagggcttg ccggctactt tggtgatgat ctctcccagc agagtaaaac cagtaccact
     2521 gtaggcccat tgggcgcccg gcttaaagtt gagtcctccg tctacgagtg cccttataaa
     2581 ttccgcgtca gtgtattcaa gtttgcgatc cattttggca ggtggaaaaa aaggatagtc
     2641 gcgaaagtga tccttgattc cggaagtgtg agacaagagc tgccgtatgg tgatcgcttg
     2701 ccaactctcg ggcagtccgt cgaagtattt gctgattgga tcttcaagac tgatttttcc
     2761 gtcctggacc aaaagcataa tggcgctggc cgtaactgac tttgtcatcg aagcaatcgc
     2821 gaattcggtg tcggcagttg caggaacgtt cagttcgaca ttcgcaagtc cgtaggtttg
     2881 atccttgacg atgcgattat gttggaccac gcaaagggat agaccaggta tgtgctgcac
     2941 agccatttct gttttgatgt atgcatcaat ttgatcgccc tgggccgtga cgggaaaagt
     3001 aagtaggagg attacggaga gcagtactat gaatcggggg ttcatcttgg acgtggctcc
     3061 tttcgaatga gtagacaggt tataagtcca gcagatctat tcggctcttc tgaaactccc
     3121 attcaacgca gagacacgga aacgcagagt ttaggatctt cgtccgcaag ccccgtttag
     3181 ctagcgatgg gggatcggct cagtaccgtc ggaatgtccg atatgcttca gcttgtcggt
     3241 catgatgcac actcaagttc agaggaacga gtcaacgacc accgacaagc tgaagcatat
     3301 cggacattcc gctctgcgga tggtcctcag tgaaagtttt tgatcagcac gacgtccaga
     3361 cttgagttgc cacgtgagat agccagccgt ttaccatcgt gtgaaattgc aaagttcgaa
     3421 atcaaatcag aggtgaagtt ggttatctgt ttcggagcgc ttccgtcgat aggttggctc
     3481 cacaaattgc caacacctct ctggtccgaa ttcacgatga tcgcgcgtcc atccatggtc
     3541 cacgcaatag acgaccaccg cagattgaag tgcgttggtg gagctataat tttcgtggcg
     3601 ctcgagccat cgatcgtaac cactgcaagc tgccacggtg gagtgcctcc tttaacaaat
     3661 tgaaccgttg ctatcgactt gccatctggt gaaaatctcg ccaatggagt tcgcacttcc
     3721 gtcactcgtt ccgcttcccc tccctcgctc ggtaccttcc aatacacctg cgtgcccgag
     3781 cgccacgagc agaacaagac ccatttaccg tcgggcgaga cagttgggtg aaagtctgca
     3841 aagtcgcctg aagtaatttg cttcagatcc gtgccgtctg tgtttatgcg ccatatgtgc
     3901 ggagtgccgc tacgcatcga catgaagtaa atcgtcttgc cgtccgcagc tacggccgga
     3961 tccgaggctg gctccgactg ctttaagatg gaccgcatct ctgtgccatc gagttttacg
     4021 ctgacgagtt cacgactttc tccggtatca gtaaagtaaa cgatgcgatc gtctggtgac
     4081 caatccacac cgccggtgcc gtcgccctcg ccgtgagtga tttcaactga ttgagccgtc
     4141 ggctctccga agacgctggt ccaaatctga tatgagggtg tactttgaac cgtaacaatt
     4201 gttttgctgt cgccgctgac tccaaagctc actgcgccat acccgttcag gtcgtttgtt
     4261 acgcgtttcg ctgcgccgtc aggttgagaa atgaaccaaa cttgagtccc ttgagtaccc
     4321 tcgcctatcg gcgcaatgag cccagatcca tttttcaacc atacaac
//
LOCUS       DASSFP010000032         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1645748, whole genome shotgun sequence.
ACCESSION   DASSFP010000032 DASSFP010000000
VERSION     DASSFP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645748"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..756
                     /locus_tag="VFH91_00755"
     CDS             106..756
                     /locus_tag="VFH91_00755"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET6861533.1"
                     /translation="MRFTQTLFWPTTMRTILRKRRMVAVLISVVSVIPLASAQNVIEK
                     EQVMSFESATQPATSLDVVVTRVFDAPVEEVWKAWVDAEKVMRWWGPTGFTSPVARID
                     FRVGGKSLVCMRAPKEFGGQDMYNTWTYQKITPYESIEFIFHFSDKDGNKVDPSSMGV
                     PPGVPAEVPHIITFKRLGDRKTEMTVRESGYTSEQGVAISKAGLEQCLDKMALLFK"
     gene            766..1551
                     /locus_tag="VFH91_00760"
     CDS             766..1551
                     /locus_tag="VFH91_00760"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET6861534.1"
                     /translation="MVQPKPRQARRTVKKVVGKNPSNISVKEVLSSLEKLSNQRIRDE
                     LMPRYGIHTEKAFGIGMANIHELARKIGRNHELALSLWQTGWYEARLLAAFVDNPAEV
                     TTTQMDQWCRDFDNWGVVDTICFKLFDKTPFAFAKVAQWSKRKAEFEKRAAFALLASL
                     SVHDKTAPTEAFERCLPLIEKAANDDRNFVKKGVSWALRTVGRRNPELHSQAVELAGR
                     LSISSNASERWIGRAAVKELTSAPVLSRLKARQVVASKRKKKS"
     gene            1548..2045
                     /locus_tag="VFH91_00765"
     CDS             1548..2045
                     /locus_tag="VFH91_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6861535.1"
                     /translation="MTSSTELEISTPTDREVVLKRTFDAPRELVFEALTTPELLKRWY
                     GPTGWTLILCEIDLKVGGNWHFVVQRPDGKKIGQKGVYHEVISPERIVNTESWEDWNP
                     GETLVTTTLVEVERKTTLTSTSLFPSKEVRDIILKSGLADGAKQSYERLADLLRSVRE
                     RGNVG"
     gene            complement(2128..2682)
                     /locus_tag="VFH91_00770"
     CDS             complement(2128..2682)
                     /locus_tag="VFH91_00770"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HET6861536.1"
                     /translation="MSDDAIVWKSIFLNGHEYCRVLHDASFNFLVGSAIFSKDQRPTR
                     LDYSIKCDSHWRSLSAEVSGWLGDEQVDLQIFVKDGRFWFLNGEEQKELEGCVDVDLN
                     FSPSTNLLPIRRLNLRVGEVAEVKAGWLRFPSFKLEPLRQSYRRIDETTYRYESGGGT
                     FGKDLRVNPMGLVTSYPDIWEVER"
BASE COUNT          758 a          724 c          720 g          633 t
ORIGIN      
        1 cccctcgaaa gaagctcgcg atggagcgtt gcagtcgggc atggaccgtg gacttgaagc
       61 gggctacgaa cggctcgagc aaatactcgc agaggagcaa accaaatgag atttacgcaa
      121 accctttttt ggcccacaac aatgcgcacc atcttgcgga agcgcaggat ggttgctgta
      181 ttgatttcag tggtatcggt aatccctctc gcgagtgcgc agaacgttat tgaaaaggag
      241 caagttatgt ctttcgaaag tgcaacccaa ccggcaacca gcctggacgt agtagtaacc
      301 cgtgtctttg acgcacctgt cgaagaagtt tggaaagcat gggtcgatgc tgagaaggtc
      361 atgcgttggt ggggaccgac cggcttcacc tccccagtag cacgcatcga ttttcgcgta
      421 ggcggaaagt cgctcgtttg tatgcgagcc ccaaaggagt ttggcggtca ggacatgtat
      481 aacacctgga cctatcaaaa aataacgccg tacgagtcaa tcgaattcat cttccatttc
      541 tctgacaagg acggaaacaa agtcgatcct tcctcgatgg gagttccgcc aggcgtgccg
      601 gcagaagttc ctcatatcat cacgttcaag cgactcggag atcgtaaaac cgagatgaca
      661 gtgagggaat ccggctacac cagtgagcag ggcgtcgcaa tctcaaaagc cggacttgaa
      721 cagtgtctcg acaaaatggc cctgctcttc aagtagacta tcgttatggt ccaaccaaaa
      781 ccgcgccagg caagacgcac tgttaagaag gttgttggca aaaacccttc caacatcagc
      841 gtaaaagaag tattgagttc gctcgagaag ctgagcaacc agcgcattcg cgatgaattg
      901 atgcctcgct atggcatcca cacagaaaag gcgtttggca ttgggatggc aaacattcat
      961 gagctggcgc gaaaaatcgg ccggaaccat gaactcgccc tttcgctctg gcaaactggc
     1021 tggtacgaag cacgactcct tgctgcgttt gtcgataacc cggcagaagt caccaccact
     1081 caaatggacc agtggtgtcg cgacttcgac aactggggcg tcgttgacac tatctgtttc
     1141 aagctatttg ataaaacacc tttcgctttt gctaaagtcg ctcaatggtc taaaaggaag
     1201 gccgagtttg aaaagcgtgc cgcgtttgct ttgctcgcgt ccctttcggt acatgataag
     1261 acagcgccaa cggaagcctt cgaacgctgt cttccactta tcgagaaagc agcgaacgat
     1321 gatcgtaact tcgtaaagaa aggcgtgagt tgggcactca gaacggtggg ccgtcgcaat
     1381 ccagaactgc atagtcaggc cgtcgaactt gccggccgcc tttctatttc ctcaaacgcc
     1441 agcgaacgct ggattggaag ggctgccgta aaggagctta cgagtgcgcc ggtcttaagc
     1501 aggttgaagg cgcgtcaggt agttgcatcg aaaaggaaga agaaatcatg acatcgtcta
     1561 cagagctcga gatatcaacg cctactgatc gcgaggttgt attaaagcgg acctttgatg
     1621 cgccgcggga gctagtgttt gaagcactga ccacacccga gctgctgaaa cgatggtatg
     1681 gtccaacggg ctggacgttg atactttgtg agattgattt gaaggttggc ggcaattggc
     1741 actttgttgt gcagagaccc gacggaaaga agattggtca aaagggtgtc tatcacgaag
     1801 ttatttcacc tgagcggata gtcaacactg aatcatggga ggactggaat cccggtgaga
     1861 cgctggtaac cacaactctt gttgaagttg agcgaaagac gacgttgaca tctacgagct
     1921 tgtttccatc gaaagaagtg cgcgacataa ttctaaagtc aggcctcgct gatggtgcga
     1981 aacagagtta cgaacggtta gcagacttgt tgaggtcagt acgggagcgc ggtaacgtcg
     2041 ggtgagcctc aacatgtcat caagtagcga taacacaagg ctcagccgac gctatcgcgc
     2101 tccggtactg acccttacta cacgacttca gcgctcaacc tcccagatgt ccggatagct
     2161 cgtaaccagg cccatggggt tgactctcag atccttcccg aatgttcctc caccactctc
     2221 ataacgataa gtagtttcgt ctattcgtcg ataactctgc cgaagcggct ccaacttaaa
     2281 actgggaaag cgcaaccatc ccgctttgac ttctgccacc tcaccaacac gaaggttaag
     2341 ccgtcgaatc ggtaggagat tggtcgaggg actgaaattc agatcaacat caacacagcc
     2401 ttccagctcc ttttgttctt cgccattgag aaaccagaac ctaccatcct tcacgaaaat
     2461 ctgcagatcc acttgctcat ctccaagcca gccagaaact tcagccgaca gcgaccgcca
     2521 gtgcgaatca cactttatcg aatagtccaa acgagtcggt cgctggtcct tcgagaaaat
     2581 cgcactaccg accaggaaat taaagcttgc atcgtgcaaa acgcgacagt attcatggcc
     2641 gttcaagaat atgctcttcc aaacaatggc atcgtctgac acgcacaacc tccttacttc
     2701 atctcaatca aggccagacg tgaagcttcg agtgttttta attcatcctt gttcagtttt
     2761 ggatagttga gatccagtga ggacaacgtc tcaaccaggg ccgaagcaac cacgaggcga
     2821 gtaaaccatt tattg
//
LOCUS       DASSFP010000033         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_901000, whole genome shotgun sequence.
ACCESSION   DASSFP010000033 DASSFP010000000
VERSION     DASSFP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901000"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFH91_00775"
     CDS             complement(<1..542)
                     /locus_tag="VFH91_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET6861537.1"
                     /translation="MSATLGFVIILVLLALVFDYINGFHDAANSIATVVSTRVLSPGL
                     AVVWAAFFNFIAFAVFGTRVAKSIGDGVRMDLITPELRLYVLLAALLGAVVWNLITWY
                     LGLPTSSSHALLGGYAGAGIAAYGGISGLLKSDVWLRTLKFIVLSPLIGMVIGFSLMV
                     IVYWLFRGFSASRVDKIFRRG"
     gene            complement(600..1223)
                     /locus_tag="VFH91_00780"
     CDS             complement(600..1223)
                     /locus_tag="VFH91_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET6861538.1"
                     /translation="MGFLNLLPKEEQYFDLFTQMTLYISAAARELKEMLADKNRDYAE
                     YAQRIKGLEHACDELTHNISTRLNKSFITPFDREDIYTMSTALDDIVDLIDDAARAII
                     ILNIQEITDYARDFSLVIERMAEQLREIVSTLQRPKNVTQRLVEIHRLENEGDDIYHA
                     AIADLFHDPPDALTVLKWKEVYEKLEAAVDRCENVANIIESVIIKHT"
     gene            1320..2294
                     /locus_tag="VFH91_00785"
     CDS             1320..2294
                     /locus_tag="VFH91_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET6861539.1"
                     /translation="MNHFAEVQNLLQIEAQALAETANRLDPEHLERVMALLAACKGKV
                     VILGVGKSGIIAQKIAATMTSAGTAAIHLHPSDALHGGLGILQIDDVVIILSNSGETD
                     EVVAMLPFIKHKQVPVIAIVGNLNSTLARRADAVLDASVDQEACPLNLAPTTSTTVAL
                     ALGDALAMTLMKLKGITSDQFASNHPAGQLGKRLTLRVTDLMHADNANPVIPIGSSWV
                     EVVKAISKGGLGAVCVVDQSGRLAGIITDGDLRRAIEHTDHDGLSSLTCDQFMTAKPA
                     VAKPEMLAFDALRLMEDRRSQISVLPVVDDREVCVGLIRVHDIVRSGL"
     gene            complement(2348..2767)
                     /locus_tag="VFH91_00790"
     CDS             complement(2348..2767)
                     /locus_tag="VFH91_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6861540.1"
                     /translation="MPIDETSQLMADQLEERLIDFAVRIIKLSNCLPRTPAGKHIANQ
                     ILRSGTSPAPNYGEARGAESRADFVHKIRIVVKELNETSIWLRIIQRSHLLSESSLLG
                     ITEECRELCKIFTSSLQTIRRGKHRSVDTGTRQKNEK"
BASE COUNT          758 a          694 c          760 g          720 t
ORIGIN      
        1 ccacgccgga aaattttatc cacccgtgaa gcggaaaaac cacgaaacaa ccagtagacg
       61 atcaccatta aggagaagcc gattaccatg ccgatgagtg gcgacaaaac gataaacttt
      121 aaagtgcgaa gccaaacatc tgatttcaat agaccactga ttccgccgta cgccgcaatc
      181 cccgcacccg catatccacc cagcagagcg tgagagctcg aggttggtaa gcccaaatac
      241 cacgtgatca agttccagac gacggcgcct agtagagctg ccaacaaaac atagagtcgc
      301 agctcaggag taatcagatc catcctcacg ccatcgccga ttgacttggc gacacgagtt
      361 ccaaacacag caaaagcgat gaagttgaag aatgcggccc acacaaccgc gagacctgga
      421 gaaaggacgc gggtggaaac gacagtcgca atcgagttgg cagcgtcgtg aaagccgttg
      481 atgtaatcga agaccagagc gaggaggacc aggatgatga cgaagccgag cgttgcagac
      541 atagtcgagt cagaaaaaga ccggtattgt tcaattcgcg gccgtcagtc agtatcctct
      601 taggtgtgtt tgataattac gctttcgatg atgttagcta cgttctcgca acgatcaact
      661 gcagcctcga gtttttcgta aacttctttc cactttagga cggtcaaggc atccggtgga
      721 tcgtgaaaaa ggtccgcaat tgctgcgtga taaatgtcgt cgccttcatt ctcgagccgg
      781 tggatctcga ctagccgttg agtgacgttc ttgggccgtt ggagcgtaga aacaatctcg
      841 cgaagttgct cggccatccg ttcgattacg agggagaaat cgcgagcgta gtcggttatc
      901 tcctggatat ttaagattat tatcgcgcgg gctgcatcgt cgatgaggtc cacgatatcg
      961 tccaaagccg tcgacatcgt gtatatatct tctcgatcaa acggagtgat gaaggattta
     1021 ttgagacgag ttgagatgtt gtgtgttaac tcgtcacaag catgttccag gcccttaatt
     1081 cgttgcgcgt actcggcata atcgcggttt ttatcggcta gcatctcttt cagctcgcga
     1141 gcagcggcgg agatgtagag tgtcatttga gtgaagaggt cgaagtactg ctcttctttt
     1201 ggcagaagat taagaaagcc catagtgatg gcgaatgtta gagcctgaat ttgatctgaa
     1261 caggccgggg aatataacgt aggacgggtc cacttgcaaa aggatagctt tgtaaaccta
     1321 tgaaccattt cgcagaagtt cagaacttgt tgcaaatcga agcgcaagca ctcgcggaaa
     1381 ctgctaaccg ccttgatcca gaacatctgg agcgggtgat ggcgctcctg gcggcctgca
     1441 aaggcaaggt ggtgatactt ggcgttggca agtctggaat catcgcccag aaaatcgctg
     1501 caaccatgac gagtgctgga acagctgcga tccatctaca cccttcggat gctttacatg
     1561 gtggacttgg cattctccag atcgacgatg tggtgatcat tctgagcaac agcggagaga
     1621 cagacgaagt agtcgcaatg ttgccgttca ttaagcacaa gcaggttcca gttatagcga
     1681 tcgtgggaaa tctcaactca actctggcaa gacgtgcgga tgcggtgctg gatgcttctg
     1741 tcgatcagga ggcgtgtcca cttaatcttg ctccaaccac aagcactaca gttgcgctcg
     1801 cgctgggcga cgctctggcg atgacattga tgaaattgaa aggtattacg tcggatcagt
     1861 ttgccagcaa tcatcctgcg ggccaacttg gcaaaaggct gacactgcgc gtaacggatc
     1921 tgatgcacgc cgataacgcg aatcccgtta tacccatcgg gtcatcgtgg gttgaagtgg
     1981 taaaagcgat cagtaaaggc ggtctcggcg ccgtttgtgt cgtggatcag tcaggccggc
     2041 tggcggggat cattactgat ggagatttgc ggcgggcgat tgaacatacc gatcatgatg
     2101 gtctatcaag tttgacctgc gatcaattta tgactgcaaa gccggctgtc gcaaaaccag
     2161 agatgcttgc ctttgacgcc ctacgattaa tggaagatcg ccgctcacaa atatccgtgt
     2221 tgccggtcgt cgatgaccgc gaagtctgcg tcggcctcat ccgcgtacac gacatagttc
     2281 gcagcggcct ttaacagcgt tattggtcac tgttcctctt cattttccat ttttcatttc
     2341 tcatttctca tttttcattt ttctgccgcg tgccggtgtc taccgatcta tgcttaccgc
     2401 gacggattgt ttgcaacgag ctagtaaaaa ttttgcacaa ttcacgacac tcctctgtta
     2461 tgccgagtaa cgaagactcg gatagaagat gagatcgttg aattattcgg agccagatcg
     2521 aagtctcatt cagttctttg accacaattc ttatcttgtg cacgaagtcc gcacgacttt
     2581 cggcgccgcg agcttccccg tagttaggtg caggtgatgt tcccgaccgt aagatctgat
     2641 ttgctatgtg ctttcctgcg ggagttctgg gcaagcagtt tgagagctta atgatgcgaa
     2701 ctgcaaaatc gatcaaacgc tcttcgagtt gatcggccat caattgtgag gtttcatcaa
     2761 tcggcatgtg aggttaccgt cctgattagc taacaaagat agtccgatat tttctttatc
     2821 tacttgaatg aagtcaaccc tactgacgca cttcaagcat ggcggtattt gcaactcatc
     2881 gaaatgagaa tgaaagaatg aaaaatgaga aatgagaaat gaaaaatggt cc
//
LOCUS       DASSFP010000034         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1347974, whole genome shotgun sequence.
ACCESSION   DASSFP010000034 DASSFP010000000
VERSION     DASSFP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347974"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFH91_00795"
     CDS             complement(<1..875)
                     /locus_tag="VFH91_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HET6861541.1"
                     /translation="MIKTSRRCGNRCCSLLLIGLFFVLANAQDTLDVWKDFDFSKTTL
                     KQAQVQALSIEDLKLLRGIVFGRHGRVFKDAEIKAYLESQSWYKPNAEFSNSMLNDTE
                     RRNLDLIRVAEAGKHDTIQPGDMRYWTQRTITPKKLGKHSGAEWKVLIAEVEAIHGKR
                     FDDEPWLQRYFEERYWYFPSAQYDPKNLTVIERKNVEALTAAQKRQRNVALLPGDMEL
                     FENRSISDKMLQGLSLHELRLLRNEVYARHGRSFRAEWLQQYFFQQPWYAPDKNFKDE
                     QLSGNDQTNVETIVRR"
     gene            complement(906..2024)
                     /gene="dnaJ"
                     /locus_tag="VFH91_00800"
     CDS             complement(906..2024)
                     /gene="dnaJ"
                     /locus_tag="VFH91_00800"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6861542.1"
                     /translation="MSKRDYYEILAVSRTATDQEIKSAYRRLAVKYHPDKNPNDASAE
                     ERFKEAAEAYSVLSDADQRKKYDRFGHAGVSSGAGSGAWGAPGFGGIEDILGDLFGFG
                     DVFGGSRSGGRRSAAQRGADLRYDLEISLEEAATGMTAQLRIPRLESCDTCKGSGAAA
                     GTQPETCTTCSGTGQVRYQQGFFSVARTCHACRGAGQIVKNPCTACHGAGRVEREKQM
                     EVKVPAGVETGSRLRIQGEGESGTQGGGPGDLYVVIHVTEHEVFERQGSNLYEAVPVT
                     FAQAALGAEIMVKTLEGEDKLKVPMGTQTGTVFRLRGKGMPVLSGRGRGDLFVSVTLI
                     TPTSLTREQRRLLEQLAEVESKDLDDKGLVDKVRNIFS"
     gene            complement(2078..>2617)
                     /locus_tag="VFH91_00805"
     CDS             complement(2078..>2617)
                     /locus_tag="VFH91_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6861543.1"
                     /translation="SVEIHVLQGEREMAEGNRSLGKFELVGIPPSPRGVPQIDVVFEV
                     DANGIVSVSARDKATGHEQQMRITPTSGLAPDEIDRLISEAEHFADTDKGAKELIVLR
                     NKLESLLRNTQKSYGKFGGLLSQNDQERAERVFTEAESASKGQDLSDINKALNALERT
                     AGQLTTAMMNPNPDSKSET"
BASE COUNT          600 a          728 c          600 g          689 t
ORIGIN      
        1 cgtctaacta tcgtttcgac gttcgtctgg tcgttaccgg agagttgctc gtccttaaag
       61 tttttatcag gcgcgtacca cggctgttgg aaaaagtact gttgcaacca ctcggcacga
      121 aacgagcgac catgacgagc gtaaacctca ttccgtaaca ggcgaagttc gtggagactt
      181 aaaccctgaa gcatcttgtc agagatcgaa cgattctcga acaactccat atcgcccggc
      241 agaagtgcaa cgttgcgttg gcgtttctgt gctgcggtaa gggcctccac gttttttcgc
      301 tcgatcactg tcagattttt cggatcgtat tgcgcactgg gaaaatacca gtaccgctct
      361 tcgaaataac gctgaagcca tggttcatcg tcaaagcgct taccgtggat ggcttcgact
      421 tcggcaatca gcaccttcca ttcagcacca ctatgtttgc ccaatttctt tggcgtaatg
      481 gtgcgctgtg tccaataacg catatcgccg ggctgaatcg tgtcgtgctt accagcctct
      541 gcgacgcgaa tcaggtcgag gttgcgtcgt tcagtgtcgt tcagcattga gttgctgaat
      601 tcggcatttg gtttgtacca ggactgtgac tccagatacg ccttgatttc agcatctttg
      661 aaaacacggc catgtcgccc gaaaacgatt ccgcgcaata acttcagatc ctcgattgat
      721 agcgcctgga cctgagcttg cttgagcgtg gtcttactga aatcgaaatc cttccagacg
      781 tccagagtgt cttgcgcatt ggcgaggacg aagaatagac ctatcaataa aagagagcag
      841 caacgattac cacaccgtcg ggaggtttta atcatcatct tctcactttc gcaatcggac
      901 cgagactaac tgaaaatgtt gcgtactttg tcgaccagac ctttatcatc gagatctttg
      961 ctctcaactt cagccagctg ctccaacaaa cggcgctgtt cccgagtcaa agaagtcggc
     1021 gtaatcagcg ttaccgagac aaatagatcg cctcgacccc gtccacttag aaccggcatt
     1081 ccctttcctc gcaatcgaaa gacggtacca gtttgcgttc ccatcggaac cttgagtttg
     1141 tcttcgccct cgagtgtctt aaccatgatc tctgcaccga gagcggcctg agcaaatgta
     1201 accggcactg cctcgtacag gttgctacct tgtcgctcaa agacctcatg ctccgtaacg
     1261 tggataacca cgtagagatc cccaggtccg ccaccctgag taccggattc tccttcgcct
     1321 tgaattcgaa gtctggaacc cgtttcaacg ccggcgggaa ccttaacttc catttgcttc
     1381 tcacgctcta cgcgaccggc accatggcag gcagtacatg gattcttgac gatctgcccc
     1441 gcaccgcgac aggcgtgaca agtccgagca accgagaaga aaccctgctg ataacgcact
     1501 tgtccagttc ctgaacaggt ggtgcaggtt tcgggttgcg ttcccgcggc agcccctgaa
     1561 cccttacaag tgtcacaact ctcaagacga gggattcgaa gctgtgccgt catgcccgtg
     1621 gcggcttctt caagtgaaat ttctaaatcg taacgaaggt ctgctccacg ttgagcagca
     1681 gagcggcgcc caccgctgcg ggatcctcca aacacatcgc caaagccaaa caggtcgcca
     1741 agtatgtctt cgattccgcc gaagccgggc gcaccccagg cgccacttcc tgcgccactt
     1801 gaaacacccg catgtccaaa gcgatcgtat ttttttcgtt ggtcagcgtc cgacaaaacg
     1861 ctgtaagctt ccgccgcttc tttaaacctc tcttctgcac ttgcatcgtt agggttcttg
     1921 tcgggatggt acttaacggc aagacgtctg tatgcgcttt tgatttcctg atctgttgcc
     1981 gtgcgactta cggctaaaat ttcgtaataa tctctcttac tcattcgatc ctatttttaa
     2041 cctctcgaaa atatcgactg aagactttaa atatgattta ggtttctgat tttgaatcgg
     2101 ggttgggatt catcattgcg gtagtcaact ggccggcagt tcgttccaaa gcatttagag
     2161 ccttattaat atcagacagg tcttgtcctt tactagcgga ttccgcctcg gtaaagactc
     2221 gctcggctct ctcctgatca ttctgcgaca gcaatccgcc gaacttgccg tacgactttt
     2281 gagtatttct caaaagcgat tcaagtttat ttcgcagaac gattaattct tttgcaccct
     2341 tatcagtatc agcgaagtgc tccgcctcgg aaatcaagcg gtcaatctcg tccggcgcca
     2401 aacctgaggt cggagttatt cgcatctgct gttcgtgacc agttgcctta tcacgtgcag
     2461 acactgaaac gattccattc gcatcgactt caaatactac gtcgatttgt ggaacgcctc
     2521 tcgggcttgg aggaattcct actaattcga acttgcccag cgaccgattt ccttcggcca
     2581 tctcacgctc accttgtaga acgtgaattt ctactga
//
LOCUS       DASSFP010000035         7244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_7920, whole genome shotgun sequence.
ACCESSION   DASSFP010000035 DASSFP010000000
VERSION     DASSFP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7920"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..3282
                     /locus_tag="VFH91_00810"
     CDS             142..3282
                     /locus_tag="VFH91_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6861544.1"
                     /translation="MNILPGTHLGRYEVLSKLGAGAMGDVYLARDSNLNRTVALKVLP
                     RNLAADAGRMLRFSQEAKAISALNHPNIITIHEVANIDSTHFIAVEYVKGLTLREKLS
                     EKEISAKEMIEIAEQVARALSAAHEAGIVHRDLKPENVMVRHDGYVKVLDFGIAKLSE
                     HADIPLGTAVVGEGIEGTKAGALLGTPSYMSPEQCRGAKNLDERSDIWSLGVVLFEMV
                     TGRLPFRARDFSQLLVLIAGPKDAPLEYLRGKAPQGLERIICKALQKRRRDRYQKVIE
                     LAEELRELKYELALEEKVRTSQRRSEGGISAPLNFSSIRVNDSHDTEHPTLHISSPTT
                     SPEVHPNNLSVLLNPLIGRDTEVAAVKKLLLNQEVRLVTLTGPGGTGKSSLCLRLARE
                     LLDEFQHGVFFVALTSINDPDLVAPTLAKVLGVKEERGVPLIDSMKEFFRGKHILVVL
                     DNFEQVISAAPLITDLLTSSVSLEFIVTSRAALHLRGEHEFAVLPLAVPDPNRLHPWQ
                     QLAQYSSVALFISRAQALKPDFALTEENASAVAEICSRLDGLPLAIELAAARIKLLPP
                     AAMLKRMDSSLKVLTGGARDLPARQQTMRGAIAWSYDLLESQEKTLFNRLSVFLGGCN
                     LEEAEAVCNSTSDLEIDFLDGVTSLVDKSLLRQKEQDDAEGRFSMLQTIREFGLEQLA
                     QSGELETIRSHHLNFYLEMAEQAEPELTGASQASWFSRLDKEHDNFRAAIIYSRESDR
                     PEDGLRLAGALWRFWEVRGHVGEGRAVLDQLLMLPGATEVSPNVRAKALNGVGVLAGN
                     QGDHARQTAILQESLKLYEELGDKSGIAQSINNLGSIAWSQGDYVQAQVFYTQSLEIR
                     RAISDSWGTANSLNNLGGVAFVQGDCDRALALYAESLSLRRKLGDKRGISMSLNNMGE
                     VAQQQQDYGRAATLFAESLTIRNELGDRFFIVSSLHNLAEVACSRKDYERGARLFGAA
                     EALREMIGAPLPTEKRENFDRYLEEARDALGRADLKSFRTEGRVMSLDEAIKYALSEE
                     HGSIDKALKVDAYYPN"
     gene            complement(3545..6769)
                     /locus_tag="VFH91_00815"
     CDS             complement(3545..6769)
                     /locus_tag="VFH91_00815"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET6861545.1"
                     /translation="MKRLSSRVVFCLYVAIITFTSLPFVANGQIPDRNVNMVSGKTWP
                     GGDPFLQRQNEPSIAVSSRNPLHLLAGANDYRTVDLPGLPDGEENGDAWLGIFKSFDG
                     GLTWRSTLLPGYPQDKSAEGLASPNKGFQGGADPTVRGGTNGLFYLSGIVFNRDVKGL
                     GQVFVSRFIDNNNSEIADSIQYLNTSVITGGTSGQFIDKPWLAVDIPRPGAGLCQIGA
                     QSFPAGNVYLAYSIFLGGDPANNPHTAIIFAKSGDCGATWTVTKISESFKLNQGTIMS
                     LNPATGAIYVAWRQFATPNDQDAIVFAKSTDGGKSFSKGSVVSTISAFDQASTSVSFR
                     TNSFPTMTVDAAGKIYIAWAQKGVGPSGDARIVMSVSDNSNTWSPPTQVDPSPQRGHQ
                     IMPALTFAAGKLMMIFYDLREDHTIGIFEPLPDGSYQETRSPRGDLAPAPGEPAKVFT
                     DAIMDQAPAGFSPLQRRHTIDVRAWQADVSTSEPLKFDPSQRVSQYIFGSRPGSSLVE
                     QLQVNPPNLPMFAQGTKAFIGDYIDVGAATFLPTANGGWQFNTAASSNVVFHATWTDN
                     RDVRPPIGSPPDWTKYTPTGQNGGTSLFDPTQTVAPCNSNFTGSRNQNIYTARLTQGL
                     FVGSAGNTKSLGTVLVNGNAVLLQRTFSVFLQNTTGLTKSFRMTIGAPQPLGGQASFQ
                     QVGTVLTTLDVSIGPRSTISRPVFVTSTDRRAMVVVNVAEITGVGGTIKAGGLTGSVV
                     LNPDRTSPELNDPHLGGQDIATGEVYNPDITTLPIVLSVDIANVDIANVDIANVDIAN
                     VDIANPDIANVDIANVDIANVDIANVDIANVDIANVDIANASISDATWSVKNDGNTVT
                     SYSVKLLQNTAAPPGFKTQLILFKTNTPGVARNCDLGFQRQNIVIANVVNPVFSSTSD
                     LTSTAITDPSKTNATLVLAPGEQAKLTVRVLDPDRRDTVTYDIAKGLSPVLVAHGVNT
                     GDLRNGNNKTKVTLVITTTINAVPGALKSRSYSLALQAVGGIGTYSWRLVDGALPDGL
                     VLNTTTGEISGKPSVRGVFTFTVEVKDSASPQHVALRTLKITVT"
BASE COUNT         1791 a         1809 c         1888 g         1756 t
ORIGIN      
        1 cagcggcccg gcggcgcttt gtcacataat caagtacccg gataacaaag cgccgccggg
       61 ccggcgcact ccaaagactc tatgcacgtc agctaaacgc agttagaata acgctcaatc
      121 ccgattgtgc gggttacgga aatgaatatc ctcccaggca cccatctcgg tcgttacgaa
      181 gtactctcga aactcggtgc cggcgcaatg ggtgatgttt acctcgctcg cgactctaat
      241 ctgaatcgca ccgttgcgtt aaaggtactt cccagaaacc ttgctgcaga tgcaggtcgc
      301 atgctccgct tcagtcagga agcaaaagcg atctccgccc taaatcatcc gaacattatt
      361 accatccatg aagtcgccaa tatcgactcg acacatttca tcgctgtcga gtacgtcaaa
      421 ggtctgacgc tcagagagaa gttgagcgaa aaagaaattt cggcgaagga aatgattgaa
      481 atcgccgaac aggtcgcccg cgccctttca gcagctcacg aggccggtat tgttcaccgc
      541 gatctcaaac ccgaaaacgt catggtccgt cacgatggct acgtgaaggt ccttgatttc
      601 gggatcgcga agctgtcgga acatgctgac attcccttag gcactgctgt ggttggcgaa
      661 ggtatcgaag gaaccaaggc cggcgccctc cttggcacgc cgagttatat gtcacccgaa
      721 cagtgtcgtg gcgcaaagaa tctcgacgaa agaagcgaca tctggagtct cggagttgtt
      781 ctctttgaaa tggtcactgg acgactcccg ttcagggccc gcgatttttc acaactgctc
      841 gtgttgatag caggtccaaa agatgctccc ctcgagtatc tgagagggaa agcaccgcaa
      901 ggcctggagc gcataatttg caaggccctc cagaaaagac gcagggatag atatcaaaaa
      961 gtaatcgagt tggccgaaga gctccgtgaa ctcaaatacg agttagctct cgaagagaaa
     1021 gtcaggacct ctcagcgccg aagcgaagga ggcatctccg ctcccctgaa ttttagtagc
     1081 atcagggtca acgactcaca cgacacagaa catcccactc ttcacataag ctcgcctaca
     1141 acttctcccg aggttcaccc aaataatctc tcagtcctgc ttaacccact tattggacgc
     1201 gataccgagg tagccgctgt caagaagtta ttgttaaacc aggaggttcg attggtgacg
     1261 ctgaccggcc ccggcggcac gggtaagagt agtttgtgtt tgcggttagc gcgcgagctt
     1321 ttagacgagt ttcaacacgg cgtgtttttc gttgccctta cctcaatcaa cgacccggat
     1381 ctggtagctc cgacgttggc taaggtcctt ggcgtcaagg aagaacgggg tgttccgctt
     1441 attgattcaa tgaaggaatt ttttcgtggg aaacatatcc tcgttgtact cgataatttt
     1501 gaacaagtaa tctccgctgc tcccctgatc acggatctcc tgacaagctc ggtgagtctc
     1561 gaattcatcg tgaccagccg tgccgcactt catctacgcg gtgaacacga gtttgctgtt
     1621 ctgccattag cggtaccgga tcccaatcga cttcatccat ggcaacaact tgcgcagtac
     1681 tcttcagtgg ctctctttat tagccgcgct caggcactga aacctgattt tgcgttaacc
     1741 gaagagaatg ccagcgctgt tgccgaaatc tgctccaggc tcgacggact cccgttggca
     1801 attgaacttg cagccgcgcg aatcaagctg ttgccgccag ccgcaatgct aaaacggatg
     1861 gacagcagcc tgaaagtgct aaccggtggc gctcgagatc ttcctgcccg gcaacagaca
     1921 atgcgtggcg caattgcctg gagttacgac ttactggaaa gtcaggaaaa aacactcttc
     1981 aaccgtctgt cagtgtttct gggaggatgc aatctggaag aagcagaggc cgtttgcaat
     2041 tcaaccagtg atctggaaat agactttctc gatggtgtga catctctggt cgataaaagt
     2101 ctgttgcgcc agaaagaaca ggatgatgct gagggccggt tctcgatgtt gcaaacgatt
     2161 cgagagtttg gactggaaca attggcccaa agtggcgagt tggaaaccat tcgctcacat
     2221 catctgaact tctatcttga aatggccgaa caggctgagc cggaactgac aggcgcatca
     2281 caggcgtcat ggttttcgcg tctcgataaa gagcacgaca atttccgggc cgccataatc
     2341 tacagtcgtg aaagcgacag accggaagat ggtcttcgct tggcgggagc gttatggcga
     2401 ttctgggagg tgcgaggaca cgttggcgaa ggtcgcgccg tattggacca gctgctgatg
     2461 ttacctggcg caactgaggt ttctcccaac gtgcgggcta aagcgctaaa tggggtcggc
     2521 gtgttggccg gaaaccaggg tgatcacgcc cgtcagacag cgattcttca ggagagttta
     2581 aagttatatg aagagctcgg tgacaagtct ggcatcgctc agtcaataaa caatcttgga
     2641 agtattgcct ggtcgcaggg cgactacgtt caggcacaag tcttttacac tcagagtctg
     2701 gagatacgac gagctatctc tgactcatgg ggcactgcta actcgttgaa caatctggga
     2761 ggcgttgctt tcgtccaggg cgactgcgac cgagcactcg ctctttacgc ggagagtttg
     2821 agtctccgta gaaaactggg agataagcgc ggcatcagca tgtctctcaa caacatggga
     2881 gaagtagccc aacagcaaca ggactacggc agagctgcta cactgtttgc tgaaagcctg
     2941 accatccgaa atgaactagg tgatcgattt ttcatcgtta gctcgcttca caacttggct
     3001 gaggttgcat gtagtagaaa agactatgag cgtggtgcgc gactgttcgg agctgctgag
     3061 gcgttgcgag agatgattgg agcaccgctt ccgacggaaa agcgagaaaa cttcgaccgc
     3121 tatttagaag aggcacgtga cgcgctggga cgcgcggacc tgaaatcttt tagaaccgaa
     3181 ggtcgcgtga tgtcgcttga cgaggcgatc aaatacgctt tgtcagagga gcatggctca
     3241 atcgataaag cacttaaagt cgacgcctac tacccaaact aacatccaca gattacgccg
     3301 attacacgga ttcttttaac gcaaggcaac agctctttct ctccttcggt ggcggagcgg
     3361 ctcccagaat gtcgcagtca ggaaggggac catttttcat ttgtcatttt tcatttctca
     3421 tttttcattg gcgaattgag agttgagtca acttccaaat tgtaacaatg aaaaatgaaa
     3481 aatgagagat gacaaatgaa aaatggtcac cttcctgact gcgacattct gctttgtccc
     3541 ctctctacgt cactgtgatc ttcagagttc gcagtgccac gtgctgtggc gaagcagagt
     3601 ctttcacttc gacggtaaat gtgaacacac ctctcacaga aggcttacct gaaatctctc
     3661 cggttgttgt gttcagtacc aaaccatccg gcaatgcacc atccactagt cgccaggaat
     3721 acgtgccaat ccctccgact gcctgcagtg cgagcgaata agatcgactc ttaagcgcac
     3781 ccggtactgc attgatcgtc gtcgtaataa cgagggtaac tttggtcttg ttgttaccgt
     3841 tgcgaagatc cccagtgttg acaccatgtg cgactagcac cggtgagagt cccttcgcga
     3901 tatcataggt gacagtatcg cgacgatcag gatccaacac acgcacagtc agcttagctt
     3961 gttctcccgg tgcgagaact aatgtcgcgt tggtttttga cggatcagta atcgccgtcg
     4021 acgttaagtc cgaagtactg ctaaagaccg gattgacgac gtttgcaatc actatgttct
     4081 gtcgctgaaa accgagatca cagttgcgtg cgactccagg tgtgtttgtc ttgaatagga
     4141 ttagctgtgt cttaaaacca ggaggtgctg cagtgttctg caacaacttc actgaataag
     4201 acgtgaccgt attcccatcg tttttaaccg accaggtagc gtcagatatt gaggcgttgg
     4261 cgatatcaac attcgcgata tcgacgttcg caatgtctac atttgcaata tcgacattcg
     4321 cgatatcaac attcgcgata tcaacatttg cgatatcggg gttcgcaatg tctacatttg
     4381 caatatcgac attcgcgata tcaacattcg cgatatcgac gttagcaatg tccacgctca
     4441 acactatagg tagcgtcgta atatccgggt tgtaaacctc tccagtggca atgtcctggc
     4501 cgccaagatg tggatcattt agctcaggac tcgtgcgatc cggattgagc actacggaac
     4561 cagtcaatcc gccggcctta attgtgccgc cgacgccggt aatctcagcg acattcacaa
     4621 ccaccattgc ccgacggtcg gttgaagtca caaatacagg ccgcgagatt gttgagcgtg
     4681 ggccaatact gacatcgaga gttgttagaa cagttccaac ctgttgaaat gacgcttggc
     4741 cacctagtgg ctgcggagca ccgatcgtca ttcggaagct cttagtcagc ccagtcgtgt
     4801 tttgcaggaa aacggagaat gtcctttgca gtaaaacggc attaccgttt actaaaacag
     4861 tgcctaacga cttggtgttg ccagctgaac cgacaaacaa accttgcgtt agtcgggctg
     4921 tataaatgtt ttgattgcga cttccagtga aattggaatt acatggcgcg acagtttggg
     4981 tcggatcgaa taggctcgtc ccgccgtttt gaccggttgg cgtgtatttt gtccaatcgg
     5041 gaggagaccc gattggcgga cggacatctc ggttatcggt ccaggtggca tgaaacacta
     5101 cgttactgga cgcagcagta ttgaattgcc atccgccgtt ggccgtaggc aagaatgtcg
     5161 ctgcgccaac atcgatataa tcaccgatga aagccttggt gccttgagca aacatcggca
     5221 agtttggagg attaacttgc agctgttcga caagagaaga gcctggccgg ctgccaaaga
     5281 tgtattgcga cactcgctgc gacggatcga atttgagagg ttcgcttgta ctgacatcgg
     5341 cttgccacgc acggacgtct attgtgtgcc ttcgctgcag tggtgagaag cccgccggcg
     5401 cctgatccat gattgcatcg gtaaaaactt ttgccggctc acctggcgcc ggtgcgagat
     5461 ctcctcgtgg agaacgcgtc tcttgatacg agccatcagg taacggctca aaaatcccaa
     5521 tagtgtggtc ctcccgcaag tcatagaaaa tcatcatgag tttgcccgcc gcaaaagtga
     5581 gcgcgggcat tatctgatga cctcgttgag gcgagggatc gacttgggtt ggtggactcc
     5641 atgtgttgct gttatcggat accgacatga cgatgcgagc atcgcctgat ggtccaactc
     5701 ctttttgtgc ccaggcaatg tagattttgc cggcagcatc caccgtcatc gtcggaaatg
     5761 aatttgtgcg aaaggacact gaggtgctcg cctgatcgaa tgctgagatt gtcgagacta
     5821 ctgatccctt gctgaatgat tttccgccat cggtggattt tgcgaaaacg atcgcgtctt
     5881 gatcattcgg agttgcaaat tgccgccagg cgacataaat agcgcccgtc gccgggttaa
     5941 gcgacatgat ggtgccctga ttgagcttga aactctcgct tattttggtg acggtccagg
     6001 tagctccgca atcaccagac ttggcaaaga tgattgcagt gtggggattg tttgcagggt
     6061 cccctccgag aaaaatgctg tatgcaagat agacgtttcc agccggaaaa gactgtgcgc
     6121 caatctggca tagccctgct cccggtctcg gaatgtcaac tgcgagccac ggcttatcta
     6181 taaattgacc tgacgtgccg ccggtgatta cagaggtatt cagatattga atgctgtctg
     6241 cgatttcact gttgttgttg tcgataaagc gtgaaacgaa gacctgcccc agtcccttta
     6301 catctcggtt aaaaacaatt ccgctgagat agaagagacc attcgtacct cctcgtaccg
     6361 ttggatcggc gccaccttga aaacctttat taggcgaagc tagaccttca gctgacttgt
     6421 cttgtgggta tccgggcagc agtgtgctgc gccatgtcag tccgccatca aacgatttaa
     6481 aaattccaag ccatgcgtcc ccattttctt cgccgtccgg aaggcctggt agatcgactg
     6541 tacggtaatc gttggctccg gccagcaggt ggagtgggtt acgagaagag acggcaatag
     6601 atggctcatt ctgtcgctgt aagaatggat cacctccggg ccatgtcttc ccagagacca
     6661 tgttgacatt gcggtctggg atttggccat tagcaacaaa cggcagcgat gtgaatgtta
     6721 tgattgcgac gtaaagacag aatacgactc gggaggataa gcgtttcaag ggagatgctc
     6781 cttcaagatc tcatggccta atcgactgct ggggcagcga ctaagacaaa caaggttaga
     6841 actctaatat gcaccgacaa gcagttagta aatggtgcac accgtctggg caattgcgca
     6901 taccttagtg caatctcgtt tcaatagcaa cgcataatta gtaatttgaa actctgtgct
     6961 gcctctgaga tctctgtgac tctgtggtga tggttttctt aagattttgc tcaccacaga
     7021 ggcaccgagg acacaaaggt tgcacagaga aagccaagtt gggacaccac tggaatttga
     7081 gtctacttgc agggcttcaa ccttcccaac tgcatgactc tggaagatgt ctacgacagc
     7141 agtaacaata gatcgtcgcc ggtgatgttt ttgaggacgc tgttgtcggc gttgatgatg
     7201 gcatcggcga gctctcgctt ggttgattgc aacgctaaaa cctt
//
LOCUS       DASSFP010000036         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_453600, whole genome shotgun sequence.
ACCESSION   DASSFP010000036 DASSFP010000000
VERSION     DASSFP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453600"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFH91_00820"
     CDS             <1..447
                     /locus_tag="VFH91_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6861546.1"
                     /translation="NGRPYESTAETIEQSRLTFISLSAFAAASAQNSEFSRSVLSALN
                     EELTETVELIRMLLLARSAEEKLAQLLLKWCDEHGVAESSGIRVSNKFTQYQIAEMIC
                     VSRETVTRLLSDFSRRGVIRLMPDSIFILSRRLLSSMMSCEESAGS"
     gene            589..1590
                     /locus_tag="VFH91_00825"
     CDS             589..1590
                     /locus_tag="VFH91_00825"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HET6861547.1"
                     /translation="MNRTIFLKVLILAALLLAVGWLASTGSSHSLPVETTKPVSNSDE
                     YVGSEACKDCHEDQFKAFSHTSHAQLAKISSWNGKVTGCEACHGPGKAHLEEGDPTKI
                     ISFKNKSSKEISETCLVCHAGKEERNNFRRGEHWRNDIGCTDCHSPHLLASNRNVPQS
                     ITFVGPANAEKPGFSTIHMLKLGEPQLCVSCHGEVKPDFNKPFHHKVLEGVMKCSDCH
                     NPHGGFELKQARLATGADAACIKCHADKQGPFTYEHAPLKTEGCAVCHTPHGSANPRL
                     LRFSAVNQLCLTCHSVAHDVGAVEPAGPQHNQNAQYANCTACHVKIHGSRTSPVFFR"
     gene            1601..3835
                     /locus_tag="VFH91_00830"
     CDS             1601..3835
                     /locus_tag="VFH91_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861548.1"
                     /translation="MNTMRMQTIPVLTAALFCLCIGGSIARAQQPVATTTPYKSKPAN
                     TDSSTVDAGDNAGDYTIISTLEFGYRGQRVDGDLNKYRSDLNYKAGPRLFDSSFLMKA
                     LDGTGGLFDTFLVTTTGWGSDPNGQVRVSVENPKWYRFDGTYRKFKYFRFVNNLANPN
                     WIFSPATFSVPPNPVTGEHGYNTNTKLGDFDLTILPKNRWIRFNVGYSPERISGPAFT
                     NYHVGGNDFNLLSELRSRANDYRVGADGQVGPIDFSFLQGFRRFRDDSFISLGPTPGI
                     NLNPAVASLTSFHRDEPTRGSVNFTRFSAHTLVAQKLDITGRIVYSHSTSDFQTVENS
                     TGLNWNPRVTGWPPGPLPATPNILNLGQYNITGTSKRPNTLGDIGVTYLAFSKFRISN
                     TFRVEDFKIDGDAVFADFFSITRGSGATLRTDTVGFNNLDAHRRTRYRKYQNILEGDY
                     QFSSHYAVHFGYRYGRRRIEDSFEGFNLGSNGSLTPPNARTSDSEEESNHTHALFGGF
                     KIRPLPNWTVYFDAEHGTADNVFTRIGNYDYLNIRAKSRYSPTNRLSFNFAVISRNNS
                     NPSEIAGVSLEDFGVSIKSRIFTSSVDWNPSSRFSLNAGYNYNWLNSDAVVDYFFNSI
                     RHPLGRSLYYVRNNFLFIDTTILLAPRVTLFAAYRVNDDNGQGNRLANPTGTPGFLVS
                     SYPMTFQSPEARLAIKINRRLDWNFGYQYYNYNESTLVGPRPQNYHAHLPYTSLRLYI
                     GRKE"
     gene            3890..>4903
                     /locus_tag="VFH91_00835"
     CDS             3890..>4903
                     /locus_tag="VFH91_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6861549.1"
                     /translation="MIPITLVVLATLLVISFRVNSIEAASLRTYVDQRAQRRQNVRSK
                     PSPDYSRFSHQTHVTSGQLACGTCHNFPTENWKTVRKGDAAFPDVAEFPRHETCLNCH
                     RTQFFIRQRPAPAICSNCHVNVTPRDSTRFLFPSLGDLEGTSRSNLTPEFGVNFPHGK
                     HIDVVGKRLPEWQRVTPFAFLLIKMQDKPAATQSAEPKSCPVCHETYQPQGSSDDEYV
                     TKPPKDLGDNFWLKKGTFKTIPNSHTVCFSCHNTDAGIPPLPNECQVCHKLKSEGSSS
                     QPDFDANLLAVFGVRNKVIVDASRKRDSSGTFRHEGGEHPNISCLNCHDVQTMNTTEL
                     KTLR"
BASE COUNT         1279 a         1215 c         1153 g         1256 t
ORIGIN      
        1 aacggtcggc cctatgaaag taccgcggag acaatcgagc aatcgcgcct aaccttcatc
       61 tctctatctg catttgctgc tgcgagtgct caaaacagcg aatttagtcg ttccgtcctg
      121 tcagcattaa atgaagagtt gactgaaacg gttgaattga tccggatgct gttattagca
      181 agatccgctg aagaaaaact tgctcaacta ttgctcaagt ggtgcgacga gcacggcgta
      241 gcagagtcga gtggaattcg cgtaagcaat aaattcacac aatatcaaat cgcagagatg
      301 atttgcgtct cacgagaaac agttacacgt ttgctgagtg acttcagtag gcggggagta
      361 attcgcttga tgcccgattc aatcttcatc ttgtcgcgtc gtttgctgtc ttcgatgatg
      421 agctgtgagg aaagtgctgg cagttagaag tgttggagaa taattccgtc acaacacccg
      481 cgattgtgac gctcgtcaca gaattctcga ccatgacaac ctagaatcat tgcggcacat
      541 tctcgcctct taatctcggc ccatggcctt aactgaggag cttcgaaggt gaatcgtacg
      601 attttcctta aagtactgat ccttgctgca ctcttattag cggtaggatg gcttgcgtcg
      661 actggctctt ctcactcact cccagttgag acgactaaac cggtatccaa ctccgacgaa
      721 tacgttggca gcgaagcttg caaggactgt cacgaggatc agttcaaagc cttttctcat
      781 acctcacatg cgcaactcgc aaaaataagt agctggaatg gaaaggttac cggttgcgaa
      841 gcctgccatg gccccggtaa ggctcacctg gaagagggtg atccaacaaa gattatttcc
      901 ttcaagaata aatcttcgaa ggaaatttca gaaacctgcc ttgtctgcca tgctggaaaa
      961 gaagaacgaa acaattttag acgcggtgaa cactggcgaa acgatatcgg ttgtaccgat
     1021 tgtcactcgc cacatctttt agcgagcaat agaaacgtcc cgcaatctat cacgttcgta
     1081 ggtcctgcca acgccgagaa gcccggcttt tcgacgatcc atatgttgaa gttgggagag
     1141 ccccaactct gtgtgagttg tcatggtgag gtaaagcccg actttaataa gccgtttcac
     1201 cataaggtcc tcgaaggagt gatgaaatgc agtgattgtc ataatccgca cggtgggttt
     1261 gaattgaaac aagctcgact cgcgaccggt gccgatgctg catgcattaa atgccatgct
     1321 gacaagcaag gtcctttcac gtatgaacac gctccgctca agactgaagg atgtgctgtt
     1381 tgccatacac cacatggatc agccaatccg aggctcttga gattcagtgc tgtcaatcaa
     1441 ctttgtctga cctgtcatag cgtagctcac gacgttggcg cagttgagcc agccggccca
     1501 caacacaatc agaatgcgca atatgcgaac tgtactgcct gtcacgtgaa gattcacggg
     1561 tcacgaacca gtccagtttt cttccgctga tgacgagttg atgaacacca tgagaatgca
     1621 aacgataccg gtacttaccg cagcgctgtt ttgtttgtgt attgggggtt caattgcgcg
     1681 tgctcaacag cccgtcgcga cgactacgcc ttacaagtca aagccagcca ataccgacag
     1741 ttctactgtc gatgctggcg acaacgcagg cgattacaca attatttcaa cattggagtt
     1801 tggctatcga ggtcagcgcg tcgacggtga tttgaataag tatagaagtg atttgaacta
     1861 taaagccggg ccccggttgt ttgactcaag cttcttgatg aaagctctcg acggtacggg
     1921 aggtttgttc gatacctttt tggtcacgac gacggggtgg ggatcggacc caaacggcca
     1981 ggtacgggta agtgtcgaga atccaaagtg gtatcgattt gatggcacgt atcgaaaatt
     2041 taagtatttc cggttcgtaa acaacctcgc caatcctaac tggatatttt ctccagcgac
     2101 gttcagtgtc ccgccgaatc cagttactgg agaacacgga tacaacacaa acacgaaact
     2161 cggtgatttc gatctgacca ttctgccgaa gaacaggtgg atccgtttta atgttgggta
     2221 ttcaccagaa cgcattagtg gcccggcgtt cactaactat cacgttggtg ggaacgattt
     2281 caatttgctc tcagagctca gatctcgggc caatgattac cgtgtgggcg ccgatggtca
     2341 ggttggacct attgattttt cgttcttgca gggattcagg agatttcgtg acgatagctt
     2401 tatcagtctt gggccaacgc cgggaataaa tctaaatcct gctgtagcat ctttaaccag
     2461 tttccatcga gatgaaccga cccgcggtag cgtaaacttc acacgattta gtgcccacac
     2521 gctggttgca cagaaactcg atataaccgg tcgcattgtc tatagccatt caacttcaga
     2581 ttttcagact gtagagaatt ctacgggcct taactggaat cctcgtgtga cgggatggcc
     2641 tcctggtccg ttgcccgcaa cgccaaacat tctgaacctg ggccagtaca acatcacggg
     2701 aacttcgaaa cgaccaaata cactcggaga catcggtgtc acctacctgg cgttctccaa
     2761 gtttcgaata tcaaatacat ttcgagttga agattttaaa attgatggtg atgcagtctt
     2821 tgccgacttc ttctcaatca cgagagggtc aggtgcaact cttagaactg ataccgtcgg
     2881 tttcaacaat ctcgatgctc accgtcgtac caggtatcgg aagtaccaga atattcttga
     2941 aggcgactac cagttcagct cacattatgc cgtgcacttt ggctatcgtt atggccgtcg
     3001 acggatcgag gactctttcg aaggattcaa tttaggatca aatggttctt taacgcctcc
     3061 taatgctcgc acgtctgaca gcgaagagga gagcaatcac acccacgctc tttttggtgg
     3121 ctttaagatt cgcccacttc cgaattggac cgtgtacttt gatgcggaac atggcaccgc
     3181 tgacaatgtg ttcactcgaa tcggaaatta cgattacctg aacatacgtg caaagagcag
     3241 gtattcccct acaaatagac tctcttttaa cttcgccgta attagccgta ataactctaa
     3301 cccatctgag attgccggtg tgtcgctcga agacttcggc gtatcgatca agtcgcgcat
     3361 ctttacttcc tctgtcgact ggaatccgtc gtctcggttt tcgctgaatg ccggttacaa
     3421 ctacaactgg ctcaacagcg atgcggtggt agattatttc tttaacagca tcagacatcc
     3481 gctgggtcgc agtctctatt acgtacggaa caacttcctc tttatcgata caacgatact
     3541 tttggcgccg cgagtaacat tatttgcagc gtatagagtt aacgatgata acggccaggg
     3601 caatcgactg gcgaacccga caggaacgcc aggctttttg gtttcctctt atccaatgac
     3661 gttccaatca ccggaagctc ggctcgcgat caagatcaac aggagacttg actggaattt
     3721 cggttatcag tactacaact acaacgagag tacgttggtt ggtccacgtc ctcagaatta
     3781 tcacgctcat ctgccttata catcgttgcg tctctacatc ggtcgaaaag agtaactgct
     3841 ggcagatcaa cgccataagc tgttgtggtt tatgcagaag gacgacttag tgatccccat
     3901 aacactcgtt gtcctggcca ctttgctggt catcagtttt cgggtgaatt cgatcgaagc
     3961 agcgagcttg cgaacctacg tggaccaaag agcacaaaga cgtcagaacg ttcgctcaaa
     4021 gccttcgcca gactattcca ggttttctca tcaaacacat gtcacgagcg gccagctcgc
     4081 ttgcggtact tgtcacaact ttccgactga aaactggaag acggtgagaa aaggtgatgc
     4141 tgcttttcca gatgtcgccg aatttcctcg tcatgaaact tgtttgaatt gtcatcgaac
     4201 acaattcttt ataagacaac gaccagctcc tgcaatctgc tcgaactgtc atgtcaatgt
     4261 tacgccgcgc gattcaacac gctttctttt tccgagtctg ggtgatttag agggaacaag
     4321 tcgtagtaat cttacgccgg aatttggcgt caattttccg catggcaaac acatcgatgt
     4381 cgtaggcaag cgcttacctg aatggcagcg agtaactccg tttgcctttc tgctgatcaa
     4441 aatgcaagac aaacctgcgg caacgcaatc agctgagcct aagagttgtc ccgtctgtca
     4501 cgaaacatat caaccacagg gcagctcgga tgatgaatac gtcaccaagc ctcctaaaga
     4561 cctcggcgac aatttctggc tgaagaaggg aactttcaag acaattccaa acagccacac
     4621 tgtttgcttt agctgtcaca acacagatgc aggcattcct cccttaccga acgagtgtca
     4681 ggtttgtcat aagctcaagt ccgaaggttc ttcatctcaa ccagacttcg acgcaaacct
     4741 gttagcggtc ttcggcgtgc gcaacaaagt cattgtcgac gcttcgcgta aaagagattc
     4801 atccggaaca tttcggcacg aaggtggtga acacccgaac atcagttgtc ttaactgtca
     4861 cgacgtgcaa acgatgaata caactgagct caaaactttg agg
//
LOCUS       DASSFP010000037         6906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_7929, whole genome shotgun sequence.
ACCESSION   DASSFP010000037 DASSFP010000000
VERSION     DASSFP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7929"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="VFH91_00840"
     CDS             complement(<1..1715)
                     /locus_tag="VFH91_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HET6861550.1"
                     /translation="MSQLDPNQSIPSAQTPEEPEPPDIGPPRNTAGGMPAILETFKFA
                     RSEMGVVRGLGTLLKINQPGGIDCPGCAWPDPDRERSHFEFCENGAKHVTEEATNKRI
                     TPEFFEKWSVTQLSLQPDHWLEAQGRLTHPMLLKRDADHYEPIDWNEAFKLIAGELNS
                     LDYPDQAVFYTSGRTSNEAAFLYQLFVRQFGTNNLPDCSNMCHESSGWALSQTIGVGK
                     GTVTLDDFDRAEAIFVIGQNPGTNHPRMLTSLQRAKRNGCKLVHINPLHETGMNRFKH
                     PQQVLGLLGSGTKLADLFLQVRVNGDVALLKGISKAVLEREEQSPGSILDHKFIGELT
                     EGYEQFVAGLKNVSWQEITTHSGVARDQIEEAAQIFVSSKRTIFCWAMGLTQHRNAVA
                     NIQEIVNLMLLRGQIGLPGAGLCPVRGHSNVQGDRTVGIWEQPTAAFLDRLAAQFDFE
                     PPRHHGLDTVDAIQAMHEGKIKVFFAMGGNFLSATPDTEFTAAALRKCCLTAHVSTKL
                     NRSHLITGKSALILPCLGRTELDQQGAGPQLVTTENSMGVIQMSQGRLEPASKDLLSE
                     PQIVA"
     gene            1682..5032
                     /locus_tag="VFH91_00845"
     CDS             1682..5032
                     /locus_tag="VFH91_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET6861551.1"
                     /translation="MGLIDLGRAATFRLLVPVTCSRFTLTKVLGSMQMAYGMRSSLES
                     LHSLNCPPKAFLEISTTPDGQQTTLIDFPTRFSLKALAPLDLILKHCFCFMQSQLCAY
                     DAVNTQTLRNSEDNTMTFSRHARSLFLLAFLFASVGLTVRAQLKPGRDPNQPIDQEYT
                     NKIREYTTAPYFNSPLTDYLPASPNIPTPKAVLGDVAGAPGKLPYAEDVYKYMRMLER
                     ASPRVKVFSIGTTEEGREMIAVAVASEKLLEKMDENRARLTKLADPRTINMDEAEADK
                     LVAASFPIYYITGTIHSPETGSPTALMELAYRLVVDDSAYIRFIRDNVVTLITPVVEV
                     DGRNRMVDVYNWHLAHPNDNWPNLVYWGHYVAHDNNRDAIGLSLKLTRNVLNTYLHWK
                     PQVLHDLHESVPYLYDNTIGDGPYNAWVDPILTDEWQMIGWNNVSEMTKFGMPGVFAH
                     GNFDTWSPGYLMFIAATHNGISRLYETFGNGGADTLERTLSPSEYQRTWYKQNPPLPK
                     AKWSQRNNNNYQQTALLTSLYYFGNNSKLFLKNFFLKSKRSIDKPKNEGPAAYVFPAD
                     DPRSGNQAELLRTLQSQGVEISKSTAPITVSVRKPAGTAARPSASPSPSPTPAAKTED
                     RTLPAGSYIVRMDQPYSRIADALLDHQYWAPNDPQTDIYDDTAWTMGELANLTVVRVT
                     DAKILLGPMDRVTGDVRSAGGVNSSGSVYLVNHNADNALVTLRYRLKDVDMEAAEEPF
                     EAAGNKFNRGSFIIRKAAGDELNRVANELGIKVYAVPDAPSVKTHPVRAARIAVMHTW
                     LSTQDEGWYRVGLDQLQVPFSYISTQDVAKEADLKGKYDVILFAPVGRGAQAIINGMP
                     MYGNPLPWKKTQLTPNLGNTDETDDMRPGLGWGGLQNLQKFVKDGGLFITVDDTSDFA
                     VNYGFTAGVSVSHSQRLRAVGVILRTKTVDSSSPIAYGYGDSLAMYCSNGPVFGISNG
                     VGGRGGRPAGRATGRGTPDDPDVPQGRPVAELPEPPPKVEAWEAPPVSDEQRRNAIGL
                     IPPNQRPRVVLRYADSRDLFVSGLLDNGDEIAQRAAVIDVPSGNGHVLLFSTNPFWRG
                     QTKGSYFLVFNAIMNFDNLNAGRKLDQK"
     gene            complement(5103..5504)
                     /locus_tag="VFH91_00850"
     CDS             complement(5103..5504)
                     /locus_tag="VFH91_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861552.1"
                     /translation="MKLLSVLAALSLLCLSVMPVFAEGFSVKEYDEFHHVLHHLQHEA
                     LPKNDMATIRAKASELIQLGQRITSLGVPQGTKAENVENFKKELARFDQALAKYGADA
                     KSGTDDDLRKSYGVVHDSFETLAEMLPAKKS"
     gene            complement(5574..>6906)
                     /locus_tag="VFH91_00855"
     CDS             complement(5574..>6906)
                     /locus_tag="VFH91_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861553.1"
                     /translation="AAVSALSLPFYLLWKEWKRADWQIVVIATFLVGLILVAWSWLSP
                     GGSGTSFNRSVILMSVVLVFTAVYAVTLRQIRARDERWFCSVRAAMPWILLVGAAALF
                     FNLSTEVVYQLAYGSVAVSSVALVAIGVTLISAVICCVLFAWSGDYDPLGLPEKTRTN
                     YVYAAEVLLALLFVHIRLTLPWLFTGFFERYWPFVVMAIAYLGVVTSEALRRKKVTVI
                     AEPLSRTGAFLPLLPVLGFWVAASEVDFSLLLFLVGGLYGLLSILRRSFAFGVLAGIA
                     GNGGLWYLWHRTADFQLFQHPQLWLIPVALSVLVAAYLNEEKLSEDQLASVRYLSLVT
                     IYASSTADIFINGVENSPWLPLILSVFSLAGIFSGIVFRIRGLLFLGSVFLLLSVITM
                     IWFASANLGWTWLWYVAGIMTGATIIFMFAVFEKKRSEVLRVVEGLKDWDK"
BASE COUNT         1827 a         1835 c         1677 g         1567 t
ORIGIN      
        1 ttcgcaacga tctgaggttc gcttagaaga tcttttgagg cgggctccaa tcttccctgc
       61 gacatttgaa tgacgcccat tgaattctcg gtagtaacaa gctgtggacc agcgccctgc
      121 tgatcaagct ctgtccgtcc aaggcacgga agtattaagg cagacttgcc ggtaatcaaa
      181 tgtgaccgat tgagtttggt tgagacgtgc gcggtcaaac aacacttacg cagagccgca
      241 gctgtaaatt cagtgtccgg ggttgctgaa agaaagttcc cgcccatggc gaagaagact
      301 tttatctttc cttcgtgcat tgcctgaatt gcatcgacgg tgtcgaggcc atgatgtctc
      361 ggaggttcga aatcaaattg cgcagcgaga cgatcaagaa acgcggcagt aggttgttcc
      421 catataccaa cagtgcgatc gccctgaacg ttactgtgcc ctctaacagg acataacccc
      481 gcccctggaa gaccgatctg accgcgcaga agcatcagat ttacaatctc ctggatgttc
      541 gccactgcat tgcggtgttg cgtcagaccc atggcccagc aaaaaatggt ccgtttcgac
      601 gaaacaaaaa tctgcgcagc ctcttcgatc tgatctcttg cgacgccact gtgcgtcgtt
      661 atctcttgcc acgaaacatt cttcaggcct gcgacaaact gctcgtagcc ttcggtcagt
      721 tcgccgatga acttgtgatc gagaattgaa cccggcgact gctcttctcg ttccaaaacc
      781 gccttggaaa tgcctttgag taacgccaca tcaccattca ctcggacttg caaaaacaag
      841 tcggccagtt tcgtgccact acccaaaaga ccgagcacct gttgaggatg cttgaaacgg
      901 ttcataccgg tttcgtgcag tggattgata tggaccagtt tgcagccatt gcgtttggcg
      961 cgctgtagcg atgtcagcat ccgcggatga ttggttcccg gattctgacc gatcacaaaa
     1021 atagcttcgg cgcgatcaaa gtcatcaagc gtgacagttc ccttcccgac acctatggtt
     1081 tgacttaatg cccagccact cgattcatga cacatgttgg agcagtcagg aagattgttg
     1141 gtgccaaact gccggacgaa cagttgatat aaaaaggctg cttcattact tgttcgtccg
     1201 gaagtgtaga agactgcctg atctggataa tcgagagagt tgagttcgcc tgcaattagt
     1261 ttgaaggcct cattccaatc gatcggctcg tagtgatcgg catcacgttt aagtagcatt
     1321 ggatgtgtaa ggcggccttg cgcctcaagc caatgatcgg gctgtaggga aagctgcgta
     1381 acgctccatt tctcaaaaaa ctctggtgtg atgcgtttat tagtcgcttc ttcggtgacg
     1441 tgcttagctc cgttttcaca aaactcgaaa tgtgagcgtt ctcgatcggg atcaggccag
     1501 gcacaaccgg gacaatcgat tcctccgggt tgatttatct tgagcaaggt ccccagaccc
     1561 cgaaccacac ccatttccga gcgagcaaat ttgaatgtct cgagaattgc aggcatcccg
     1621 cccgctgtat ttcgcggagg acctatatcg ggaggttccg gctcttcggg agtttgggcc
     1681 gatgggattg attgatttgg gtcgagctgc gacattcagg ttactcgttc cggtgacttg
     1741 ctctcgattc actcttacaa aagttcttgg ctcaatgcaa atggcctacg ggatgagatc
     1801 gtcacttgag agcctacact ctctcaattg cccacctaaa gcttttcttg aaatctcaac
     1861 aacgcccgac ggacaacaaa caactcttat tgacttcccc actcgcttta gcttaaaagc
     1921 tcttgctcct cttgatctaa ttctgaaaca ttgtttttgc ttcatgcagt cgcagctttg
     1981 cgcttacgac gcggtgaaca cgcaaactct tcgaaactcc gaggacaaca caatgacctt
     2041 ttcccgtcac gctcgatccc tgttcttatt ggcgtttttg tttgcttcag tgggcctgac
     2101 cgtcagagct cagctcaaac ctggccgcga cccaaatcag cctatcgacc aggaatacac
     2161 aaataagatt cgcgagtaca ccactgcacc atatttcaac tcgccattga ccgattattt
     2221 acccgcctct ccaaacattc cgacacccaa agctgtactc ggtgatgttg ccggagcacc
     2281 gggtaagctg ccttacgccg aagacgtcta caaatacatg aggatgttgg agcgcgccag
     2341 cccaagagtc aaagttttct ccatcgggac aaccgaagag ggacgcgaaa tgatcgcagt
     2401 cgccgtggcc tcagaaaagt tgttggaaaa gatggacgag aatcgtgcgc gactgacaaa
     2461 actagccgat ccaagaacta tcaatatgga cgaagcggag gctgataagc tcgtggccgc
     2521 gtcgttccct atttattaca tcaccggaac aattcactct ccggagactg gatctccgac
     2581 ggcgttaatg gagttagcct accgactggt cgttgatgac agtgcctaca ttcggttcat
     2641 ccgagacaac gtcgtaacgc tgatcacgcc ggtcgtggaa gttgacggac gcaaccgcat
     2701 ggtcgacgtc tacaactggc atttggccca tccaaatgac aattggccca acctggttta
     2761 ttggggacat tatgttgcac acgataacaa tcgcgacgcg atcggtcttt ccttaaaact
     2821 aacccgcaac gtgctcaata cttatctgca ttggaagcca caagttcttc atgacctgca
     2881 cgaatccgtg ccctatttgt atgacaacac tatcggcgat ggtccataca acgcctgggt
     2941 tgatcccatt ctaactgatg aatggcaaat gattggctgg aacaacgtgt ccgaaatgac
     3001 taagttcgga atgcccggag tatttgctca cggaaacttc gatacctggt caccgggcta
     3061 tctaatgttt attgccgcaa ctcacaatgg tatcagcagg ttgtatgaga cgtttggcaa
     3121 tggtggagca gacactcttg aacgaacttt atcgccgagc gaatatcaac ggacctggta
     3181 caagcagaat ccgccactgc caaaagccaa atggtcgcag cgcaacaata acaactatca
     3241 acagacggca ctgttgacgt cactttatta ctttggcaat aacagcaagc tgtttctgaa
     3301 gaacttcttc cttaagagta aacggtcgat cgataaacca aaaaatgaag ggccggcagc
     3361 atatgtcttt cctgccgatg atccacgatc aggtaatcag gctgaactgc taagaacgtt
     3421 gcagtcgcaa ggcgttgaaa tcagcaagtc aactgccccg attacggttt ctgttcgcaa
     3481 accagctggc acagctgcga gaccatctgc atccccttca ccgtcgccca cacctgctgc
     3541 gaaaacggag gatcgcactc ttccggccgg aagctacatc gtgcgcatgg atcaacccta
     3601 cagccgcatc gcagacgcat tattggacca ccaatactgg gcgccgaatg atccacaaac
     3661 tgatatctat gacgacacgg cgtggacaat gggtgaactc gcgaatctaa ccgtggtgcg
     3721 ggtcaccgat gccaaaattc tcctgggacc gatggaccgc gtaacgggag atgtgcgttc
     3781 ggcaggtggg gtcaacagtt caggttccgt ctatcttgtc aatcacaacg ccgacaatgc
     3841 tctcgtaaca ctgcgttaca gattgaagga cgtcgatatg gaagccgccg aagaaccgtt
     3901 cgaagcagcc ggaaataagt tcaatcgcgg ctcattcatc ataagaaaag cggctggaga
     3961 cgaactcaat cgtgtcgcca atgaactggg gatcaaagtg tatgcagtgc cggacgctcc
     4021 gtcggtaaag actcatcccg tgagagcagc gaggattgct gtaatgcaca cgtggttgag
     4081 tactcaagac gaaggttggt accgggtcgg cctggaccaa ctgcaagtac ccttttctta
     4141 catcagcact caggatgttg cgaaagaagc cgacctaaaa ggcaaatatg acgtgatcct
     4201 gtttgcaccg gttggaagag gagcccaggc gatcatcaac gggatgccga tgtatggcaa
     4261 tcctctccca tggaagaaga cgcaactaac gccaaacctg ggcaacaccg acgaaacgga
     4321 cgacatgagg ccgggcctgg gatggggcgg tttacaaaac ctgcagaagt ttgtgaagga
     4381 tggtggattg ttcataacag tcgacgacac ttctgacttt gcggtgaatt atggtttcac
     4441 tgcaggcgta tcagtgagtc attcgcaacg cttgcgcgcc gtgggtgtca ttctgagaac
     4501 aaagactgta gattcctcca gtcccattgc ttatggttac ggcgactcgc tggcgatgta
     4561 ctgttccaat ggaccagtct ttggaattag taacggagta ggcggccgcg gcggtagacc
     4621 ggcgggcagg gcaaccggaa gaggtacacc ggatgatcca gacgttccac agggacgacc
     4681 agttgccgaa ttgcccgagc caccaccgaa agtcgaagca tgggaagccc ctcccgtttc
     4741 ggatgaacaa cgccggaatg ccatcggact gattccacca aatcagagac cacgagtggt
     4801 tctgcgttat gcagatagtc gagacctttt tgtctctggt cttttagaca acggggacga
     4861 gattgcgcaa cgcgctgcag ttatagatgt gccgagcggg aatggtcatg ttcttctttt
     4921 ctcgacaaat cccttctggc gaggccaaac aaagggaagc tactttctgg tgttcaacgc
     4981 cattatgaac tttgacaact tgaacgcagg aaggaaactg gatcagaaat aacttcacta
     5041 gaaaacgatc tgggccgccg acaatttagt gtcaggcggc ccagaacctt ttagagcagc
     5101 aactaactct tcttagctgg cagcatctca gctagcgtct caaaagaatc gtggaccact
     5161 ccgtaagatt tccgcagatc gtcgtctgtt cccgacttag cgtcagcgcc atatttagca
     5221 agggcctggt caaacctcgc aagctctttc ttaaaatttt ccacgttttc agcttttgtt
     5281 ccctggggaa ccccaagact agtgatccgt tgaccgagtt gaatgagttc actcgccttt
     5341 gcgcgaatgg tggccatatc gtttttgggg agcgcttcgt gttgcagatg atgaagaacg
     5401 tgatgaaatt catcgtattc tttcacacta aagccctcag caaagacagg cataaccgac
     5461 aagcaaagca gtgagagagc agcaaggacc gatagtagtt tcatcatttc tccaaaaggg
     5521 atatttccaa agagaatact cgaacctcgt gtctcgacac caccgactga gttctacttg
     5581 tcccagtctt tcaatccttc cacgactcgc aacacttcgc tgcgcttctt ctcgaacacg
     5641 gcgaacataa agattatcgt tgcgcccgtc ataatccccg cgacgtacca caaccatgtc
     5701 catcccagat tcgcagaggc aaaccagatc atcgttatga ccgacaacaa caaaaacacc
     5761 gacccaagaa acaacaaacc ccgtattcgg aatacaattc cagagaatat tcccgcgagt
     5821 gagaatacgc tcaatatcag cggcaaccag ggcgagtttt caactccatt gatgaagatg
     5881 tccgccgttg aagatgcgta gatcgttaca agagagagat aacgaacact tgccaactga
     5941 tcctcactca atttctcttc gttaagataa gcagcgacaa gtaccgacag cgccaccggg
     6001 attagccata actgtggatg ctggaataac tggaagtcag cagttcgatg ccacaagtac
     6061 cagagccctc cgttgcccgc aatgcctgcc agcactccaa atgcgaaaga ccgtcgcagt
     6121 atcgacagaa gaccgtatag tccccctacc agaaacagca gcagtgagaa atcgacttcc
     6181 gaggccgcta cccaaaaacc taagactggt agaagtggca ggaacgcccc cgtcctcgaa
     6241 agtggttcgg caattacggt cacctttttc cggcgcaagg cttcactcgt gacgactccg
     6301 agataagcaa tagccattac gacaaagggc caatagcgct caaaaaatcc ggtgaaaagc
     6361 catggcaaag tcagtcgaat gtgaacaaaa agcagcgcca gtaaaacctc cgctgcatac
     6421 acatagtttg ttcgcgtttt ctccggcaga cccagtggat cgtaatctcc agaccaggca
     6481 aacaaaacac aacagatcac ggccgagatc agcgtgaccc caatcgctac cagggcgacc
     6541 gaactcactg caactgatcc gtaagcgagt tgatagacca cttcggtact taggttgaag
     6601 aagagtgctg ctgctcctac cagcaaaatc cagggcatgg ctgcgcgaac cgagcaaaac
     6661 caacgttcgt ctctggccct gatctgcctg agcgttactg catagacggc cgtaaaaaca
     6721 aggaccacac tcatcagaat cacagaacga ttgaacgagg tgccgctccc acccggtgac
     6781 aaccaggacc atgccactaa aatcagccca acaaggaaag ttgcgatgac gacaatctgc
     6841 cagtctgcgc gtttccattc tttccagagc agatagaaag gcaacgacaa agccgagacc
     6901 gctgct
//
LOCUS       DASSFP010000038         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_156805, whole genome shotgun sequence.
ACCESSION   DASSFP010000038 DASSFP010000000
VERSION     DASSFP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156805"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..852
                     /locus_tag="VFH91_00860"
     CDS             175..852
                     /locus_tag="VFH91_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861554.1"
                     /translation="MVTFLFTTLLLLGLVVLAIYFWQKPASTTGLPELSPPKEPRSLF
                     DYIPAKDELTPTTMNLDHSKRAELLERANQGDRTVLSEAHQFTEPEIYEEVLNELVEH
                     SKSHSQILALMSYVSRHNLKVNKAMVDVAMEAWSQEPTKNATSKMLHTAALTDDADIY
                     RNAIESVLRYWREKKLSEISNLELEALFHAEFWVLSSNTRSSGEGFILKQTLSSARRE
                     LEERANK"
     gene            896..>2460
                     /locus_tag="VFH91_00865"
     CDS             896..>2460
                     /locus_tag="VFH91_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET6861555.1"
                     /translation="MDKLDLTTVLFAVSTFVLLIFVLIFVKRIFVNVGAREIAIKERR
                     YFGPKMPPGRVVATGREIGIQADVLKPGLHLIQFPFEKVVRKVPLIEIGADEMGIIEA
                     IDGEPMPPGRIFAPDRAQNAHNNFQDPIAFIQQGGVKGIQLRTLPPGLWPIHPYLFRV
                     SVAKATLVPQGKVGVITAADGGPLDPGRLLGKSISDHRNFQDAEQFIASGGQKGPQVD
                     ILTPGVYRILTQSGTIEGTSETKPGLFTVRLFDATVINENGIGLVEALDGAPLNPRDY
                     VAESFAGHDNFQDGHEFIGKGGQRGPQKDILLPGTYYINPLLFKVIPESAKEVKPGEV
                     AVVVSNVGKDPSDEVRRQMAAKVRQRMEREEQEQVEQSAARLDKLDDDRTAAEIRADL
                     MAGDPADRRLDEGAHEAYVVPEGYRGIQETVVGPGRYYVNTLAVSPIIIPTTNQTVEW
                     TAGEVTNTFNPFEVISKDGFTMQLEVRVVFRVKPEDAPFMVAKIGGIERLIQNVMHPL
                     IDSIFRNQASESSA"
BASE COUNT          660 a          608 c          600 g          592 t
ORIGIN      
        1 ctttgttttc aagcgctgtt aacttgagta ataggagtga tataacaaag cggcgccggg
       61 ccgccgcact ccaaggtctt gaactttttg ggtctgtcgt cgtataaaca aatacgcggc
      121 gacggattca aaaaaagcca caacttagtg ggttcttccg aagccgagac ctaaatggtc
      181 acctttcttt ttactactct gttactctta ggcctggtcg ttctagccat ctacttctgg
      241 cagaaacccg ctagcacaac cggacttccc gaattgtcac cacctaaaga acctcgtagt
      301 ttgtttgact acataccagc taaagacgaa ctaacaccta caactatgaa tcttgatcat
      361 tcgaaacgag ccgagttgct ggaacgagcc aaccagggag accgaacagt tctaagcgag
      421 gcacaccaat ttactgagcc tgagatttac gaagaagtct tgaacgagtt ggtcgagcat
      481 tcgaaatcac attctcaaat actggctctt atgtcttatg tcagcagaca taatttaaaa
      541 gtcaataaag caatggttga tgttgcgatg gaagcctgga gtcaagagcc aaccaagaac
      601 gcaacttcaa agatgcttca tacagccgct cttactgacg acgccgatat atatagaaat
      661 gctatcgaat cggtattgcg gtattggcgc gaaaagaaat tgtccgagat ttctaattta
      721 gaacttgagg cattattcca cgctgagttc tgggttcttt catcaaacac ccgcagctct
      781 ggcgaaggtt tcatactgaa acagacactc tcttcagctc gacgcgaact tgaagagagg
      841 gccaacaagt aacttcccgt caactgccta cagttgatat tgaaagagat acaccatgga
      901 caaacttgat ctaaccactg tcctgtttgc tgttagcaca tttgtactgc tcatctttgt
      961 gcttatcttc gtgaagcgaa tcttcgttaa cgttggcgca cgcgaaattg cgattaaaga
     1021 gcggcgctat tttggtccca agatgcctcc ggggcgtgta gtagccaccg gacgagaaat
     1081 cggaattcag gctgacgtac tgaagcctgg actacaccta atccagtttc ctttcgagaa
     1141 agttgtacga aaagttccac tcattgaaat tggtgcggat gagatgggca taattgaagc
     1201 catcgacggc gaaccaatgc ctcccggaag aatctttgct ccggatcgtg cgcaaaatgc
     1261 tcacaacaac ttccaggatc ctatcgcctt cattcagcaa ggcggagtca aaggtattca
     1321 gcttcggact ctacctccgg gtctctggcc aattcatcct tacctcttcc gcgtctcggt
     1381 tgcgaaggcg accctggtcc cgcaaggcaa ggtaggcgta atcactgcgg ctgatggtgg
     1441 accactggat cctggtcgac tgctcggcaa atcgatctcg gaccatcgca acttccagga
     1501 tgcggaacaa ttcatcgcat caggcggtca gaagggtcca caagtagaca ttctaacgcc
     1561 aggcgtctat cgcatactga cgcagtcggg cacgatagaa ggaaccagcg aaacgaagcc
     1621 cggtctgttt acggtacgcc ttttcgacgc cactgttatt aatgaaaacg ggattggact
     1681 ggtcgaagcg cttgatggcg cccctcttaa tccacgtgac tatgtggccg agtcatttgc
     1741 gggacatgac aacttccagg acggacacga gtttatcggc aagggcggcc aacgtggtcc
     1801 acagaaagac atccttcttc ctggtaccta ttacattaat cctttgcttt tcaaggttat
     1861 ccctgaaagc gcgaaggaag ttaagccagg tgaagtagct gtcgtggtgt cgaacgttgg
     1921 caaagatccg tccgatgaag tacgacggca gatggctgcc aaggtacgtc agcgaatgga
     1981 gcgcgaagaa caagagcagg tagaacaatc tgctgcacgc ctggataagc tggacgacga
     2041 tcgaactgcc gcggaaataa gagccgatct aatggccggc gatcctgctg accgcagact
     2101 cgatgaagga gctcacgaag cttacgttgt acccgaaggt tatcgaggta ttcaggagac
     2161 agtagttgga ccgggtcgct actacgtaaa tacactcgcg gtcagtccga ttattattcc
     2221 gacaactaac caaactgttg aatggacggc cggtgaagtt acgaatacgt tcaatccttt
     2281 cgaagtaatt tctaaggacg gcttcacgat gcagttggaa gtacgcgtcg tattccgagt
     2341 caagccggaa gatgctcctt tcatggtcgc aaagattggc ggcatcgaac gtttgatcca
     2401 aaacgtaatg catcctttga tcgactcgat cttccgcaac caggcatctg agtcatctgc
//
LOCUS       DASSFP010000039         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_601805, whole genome shotgun sequence.
ACCESSION   DASSFP010000039 DASSFP010000000
VERSION     DASSFP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601805"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFH91_00870"
     CDS             complement(<1..324)
                     /locus_tag="VFH91_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861556.1"
                     /translation="MTNIVSLILLITALSLQNQMNKPEAADKSSPRTDRNSMTAHEEL
                     VKKAKMGRIDVYFEGDSITRRWGTSDEPYKHFLANWRENFFGWNAGNFGWGGDKTQNI
                     LWRLDK"
     gene            507..>2567
                     /locus_tag="VFH91_00875"
     CDS             507..>2567
                     /locus_tag="VFH91_00875"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET6861557.1"
                     /translation="MTKNLQTAALKTEPHSTGSIKDGIIDHLFYSQARLPELATRNDW
                     YMALALTIRDRLLDHWITTVRTLMRSDVRVVSYLSAEFLIGPQMGNNLINLGIYDQVR
                     QATEDLGLDLDELLDHEEEPGLGNGGLGRLAACYLESLSTLQLPAIGYGIRYEFGIFD
                     QEIRDGWQVEVTDKWLSPGNPWEIQRPEVSYEVNLGGYTETSVDPDGKYRVRWIPHRV
                     IKGQACDTPVVGYNVGNNNLLRLWKAEATESFDFKAFNVGDYYRAVEEKVASETITKV
                     LYPNDTMNKGKQLRLEQQYFFVSCSLQDMLGINDSAGNKPDEFHKFFAVQLNDTHPSI
                     AVAELMRLLVDVRSMDWDLAWHVTQQTFGYTNHTLLPEALETWPLPLFRELLPRHLEI
                     IYEINRRFLEEVRLRYPGDEQKVQRLSLIDENDQKYVRMAHLASVGSHAINGVAALHT
                     ELLKTSVLRDFYELYPERFLNITNGVTPRRWMVLSNPRLAELITRSIGEDWVRHTDQL
                     QRLEEHTADRAFGEAWRTVKRDNKGILAKLIKDRTGVVVDPDSLFDVQVKRIHEYKRQ
                     HLNVLHVISLYQKLKNDPSFTTTPRTFVFGGKAAPGYVMAKLIIKLINSVADVINNDK
                     KVSEQLKVVFFPDFNVKHAQFIYPAADLSEQISTAGKEASGTGNMKFALNGALTIGTL
                     DGANI"
BASE COUNT          666 a          644 c          614 g          643 t
ORIGIN      
        1 cttgtcgaga cgccagagga tgttctgagt tttgtcgcca ccccagccga aattgcctgc
       61 attccagcca aagaaattct ctcgccaatt cgccagaaag tgtttgtacg gttcgtcact
      121 tgtaccccat cgtctcgtga tcgagtcacc ttcgaagtag acgtcgatac gtcccatctt
      181 agccttcttc actaactctt catgagcagt cattgagtta cggtcagttc gcggtgaaga
      241 tttgtcggca gcctcgggct tgttcatttg attttgaagc gacaatgccg ttattaaaag
      301 gatcagcgaa acgatattgg tcatatttgt cccataggtc ctataggtcc tataggtcct
      361 attcttaatt ttatgagaga tctgaaatca aactgcgata ttctcattgt cagctttaca
      421 gcttcgagct atcttgccag tcttaattag accaaacaag catttattca aattactgtg
      481 tcaaccctac tattgaggct tttgaaatga caaagaacct gcaaactgcc gccctgaaga
      541 ccgaaccaca cagcactggc tctatcaagg acggaatcat cgatcacctc ttttacagcc
      601 aggcacgctt accagagctt gccacgcgca acgactggta catggccctc gcgctcacta
      661 tccgcgatcg tctattggac cactggatca cgaccgttcg cactttaatg cgcagcgacg
      721 tcagagtcgt ttcttacctg tccgcagaat tcctcattgg accgcagatg ggaaacaacc
      781 tcatcaatct tgggatctat gatcaggtcc gacaagcaac tgaagatctc ggactcgatc
      841 tcgacgaact cctcgatcat gaagaagaac ccggactcgg caacggcggc ctgggccggc
      901 ttgccgcctg ttatctcgaa tcactctcca ccttgcagtt gcctgccatt ggttacggga
      961 ttcgttacga gttcggaata tttgatcagg aaatacgcga cggatggcag gttgaagtca
     1021 ccgacaagtg gctatctcca gggaatccct gggagattca acggcctgag gtcagttacg
     1081 aagtaaatct cggcggatat accgaaacct ctgtcgatcc cgatggcaag taccgtgtgc
     1141 gttggattcc gcaccgagtc attaaaggac aggcctgtga tacaccggtg gtcggctaca
     1201 acgtcggcaa taataatctg ctacggctct ggaaggctga ggcgacggag tcatttgatt
     1261 tcaaagcttt caatgttggc gactattatc gagcggtcga agaaaaggtc gcctcggaga
     1321 cgattaccaa agtcttatat cccaacgaca cgatgaacaa aggaaaacag ttgcggctcg
     1381 agcaacagta tttctttgtt tcctgttccc tccaggacat gttaggcatc aacgattccg
     1441 ccggcaataa accagatgag tttcataagt tttttgcagt gcaactcaac gacacacacc
     1501 catcaatcgc tgtggccgaa ctaatgcgat tgctcgtcga tgtacgttca atggattggg
     1561 atctcgcctg gcatgttacg cagcaaactt tcggctatac gaatcacaca ctgctgcctg
     1621 aagcgttgga gacatggcca ttgcctctgt tccgtgaact cctgcctcga catctcgaaa
     1681 tcatctacga aatcaatcgg cgatttctcg aagaggttcg ccttcgctat cctggcgacg
     1741 agcaaaaggt acaacgcctt tcgttaattg acgaaaacga tcagaagtac gtgcgtatgg
     1801 cccatcttgc aagtgtgggc agccatgcca tcaacggtgt tgcggcactt cacaccgagt
     1861 tattgaaaac tagtgtgctg cgcgatttct acgaactgta tccggaaagg tttctgaata
     1921 tcactaacgg cgtaacaccg cgacgatgga tggttttgag taatccacgg ttggcagagt
     1981 tgattacaag gtctattggt gaagactggg tccgccatac agatcaactc caacgattgg
     2041 aagaacacac tgctgatcga gctttcggcg aggcgtggcg gacggttaaa cgtgataaca
     2101 aaggtattct cgcaaagttg atcaaggacc gtaccggcgt tgttgtggat ccggattcac
     2161 tctttgacgt gcaggttaag cgaattcacg agtacaaacg ccagcatctg aacgtgttac
     2221 acgtcatcag cctgtaccaa aaactaaaaa atgatccgag ctttaccaca acccctcgaa
     2281 cattcgtctt cggcggcaag gctgcgccgg gatatgtaat ggccaagctg ataataaagt
     2341 tgatcaactc agtagcggat gtaatcaaca acgataagaa ggttagcgag caactcaagg
     2401 ttgtgttttt tcccgacttt aatgtgaagc atgcacagtt catttaccct gcagcggatt
     2461 tgtccgagca gatctcaacc gccggtaagg aagcttcagg cacggggaac atgaaatttg
     2521 cgcttaacgg ggctctgacc atcggaacgt tggatggggc caatatt
//
LOCUS       DASSFP010000040         3837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_454289, whole genome shotgun sequence.
ACCESSION   DASSFP010000040 DASSFP010000000
VERSION     DASSFP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454289"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFH91_00880"
     CDS             complement(<1..398)
                     /locus_tag="VFH91_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861558.1"
                     /translation="MKLLTLIESFRARLLLLLALMLGITLGVQYYFNLRAVRSNAHMI
                     VEQEQAIMTGVALGIRSLYSDQYLDKTLGDLREPLLNDQNGRVKNILVVDEAGNVRDS
                     ILAEYVPTELEDRSRRYVQFRDLPLPPLRSA"
     gene            789..2471
                     /locus_tag="VFH91_00885"
     CDS             789..2471
                     /locus_tag="VFH91_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET6861559.1"
                     /translation="MTLHSFSPKRGIVFILFIVISLSLSSVVLGKKKSSSSSRNKSAK
                     AQKGKKASARNRSSRRGGKTYARASRRGRGGRMSAREVRRQTAREQASSLKSLQRRLG
                     RPLTKRERIAEMRRIDAAHRRATLEARRRAEAARLAALARERAMRDEVQTFISKDDTT
                     GEDPEVRRVAVNALGNHAGTVVVMDPITGRVYSVVNQEWGMRRGFKPCSTIKLVTGVA
                     GLSENVIPQVDTVGDGYRLDLTAALAHSNNPFFEQVGSKIGFNKMVSYAKELGLGEKT
                     GVNMPYEYSGKLPEMKPGFIERRMFSYADGFEVTPLQLGTLVSAMANGGKLLVPQIPR
                     SAKEQSKLNPKVRRQLPITSEVWQRMVPGMVGAVNYGSGKKAYDPTQTVAGKTGTCIG
                     QNGWVGLFTSYAPLASPRLAVVVIAEGSDGRRHFPAAVAGEIYRELNHRFGTAINMQV
                     VAAADDEEKEVGDSEDGDDAALDSTATKDSTKVTAASGNSVTTAPITTKAASTETQAP
                     KNTVKRVLMPLEKKTVEPAKNNPALPTPSNLPDGSNTTGTEQRPRRAHPNQP"
     gene            2773..>3837
                     /locus_tag="VFH91_00890"
     CDS             2773..>3837
                     /locus_tag="VFH91_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861560.1"
                     /translation="MKKTIRLIALGAVAAMFALPVAAKSSVASGFASQDQCTAENKDA
                     WYASFREERTKDQPKAYDAAKKYLGCVASSSEAPTDAQKAIIDYLKKWTTAYEEGTRK
                     NKLPQLIYTDKNYPAAYALGKELVAQDPENLKVLIDLGANGYLAAGPKNPSLSADALN
                     YAKKAIQLLESGKTVDSWQPLSGKDEALAYLHYTVGYLTLETDPSGALGHLIKAAQTE
                     TPLKKSPLTYGYIAGAYESGPYAKQSDAYKACCAGKEETPESKLMLANINQVVDRMID
                     AYARAVALAGSDASVAKLKPAWNESLTSWYKFRHNDSPAGLDQMVAGILSTPLPPEPT
                     PITTLPASTPAATPIGSTSGS"
BASE COUNT          975 a          972 c          993 g          897 t
ORIGIN      
        1 gcgctacgaa gcggcgggag tggcaaatcg cgaaattgca cgtatcgcct cgaccgatct
       61 tcgagttcgg taggcacgta ctccgcgagg atgctgtcgc gcacgttacc agcctcgtcc
      121 acgacaagaa tgttcttaac ccggccgttt tggtcgttca acagaggctc tcgaaggtct
      181 ccgagagtct tatcgagata ctggtcgctg tacaggctgc gaattccgag ggcgacgcca
      241 gtcatgattg cctgctcctg ctcaactatc atgtgggcgt tcgagcgcac ggcccggagg
      301 ttaaaatagt actgaacccc gagggttatt ccgagcatca aagcgaggag caataggagc
      361 cgtgcgcgga aactttcgat aagagtcagt aatttcattc cgagactaag aggcctgacg
      421 tagtaaaggg ggttgtcgag taaagcacat ttggacctgc gggccttttg tacttggagt
      481 cgacctgtcc aatcccgcaa tttatgtata atcggttact tttttgttgc cccccaacga
      541 aagcagatgt tatattcgct tcggtctcag agcaatgtcc ccagggaccg ccgttttgtg
      601 aattcttccc cacctcaacg attgcagctg aagttcttta agtggcactg agaggatttg
      661 gtatttctca gaactgcaaa aggtttttga tatgaggcag atgcttttaa caggcttaca
      721 aaggcatcaa gaattcgacc agcgctcacc agttgagcca atcccttagg aaaggtacgt
      781 tagctccaat gactttgcat tccttttccc ctaagcgcgg aatcgtcttc attctgttta
      841 ttgttatttc cttaagtctt tctagtgttg ttcttggcaa gaagaaatcg tcatcatcat
      901 cccgcaacaa gagcgccaag gctcagaagg gcaaaaaggc ttccgcgaga aacagatcat
      961 cacgccgagg tggtaaaacc tacgcccgcg cttcgcgtag aggtcgtggt ggcagaatgt
     1021 ccgcacgcga agttcggcgg caaactgcac gagaacaggc ttcctctctg aagagccttc
     1081 aacgccgact cggccgaccg ctgactaagc gagaacgcat tgctgagatg cgaagaattg
     1141 acgcggctca tcgtcgtgcg accctcgaag ctcgacgcag agctgaagct gcccgtcttg
     1201 ccgcgttagc tcgtgagaga gcgatgcgtg atgaagtcca aacctttatc tctaaagatg
     1261 acaccaccgg cgaagacccg gaggttcgtc gtgttgcggt caacgcgctc ggcaatcatg
     1321 ccggcacagt ggtcgtgatg gatcccatta ccggccgcgt ttattcagtg gttaaccagg
     1381 agtggggaat gcgccgtggt ttcaaaccct gctcaacgat caaattagtt accggagttg
     1441 caggtctgtc agaaaatgtg attccacaag tcgatacggt tggtgatggt taccgattgg
     1501 atctcaccgc agcattggcg cactcaaata atccgttttt cgaacaagtc ggcagtaaga
     1561 tcggtttcaa taaaatggtc tcctacgcca aagagcttgg cctcggtgag aaaaccggcg
     1621 tgaatatgcc ttacgagtat tccggcaagt tgccagagat gaagccgggc tttattgaga
     1681 gacgaatgtt ttcttacgct gatggctttg aagttacgcc attacagttg ggaacgcttg
     1741 tatcagcaat ggccaatggt ggcaaactcc ttgttccgca aattcctcgt tccgcaaaag
     1801 agcagtccaa acttaatccc aaagttcgcc gccagttgcc aattacttct gaagtttggc
     1861 agcgcatggt tcctggaatg gtaggcgctg tgaattacgg ttccggtaaa aaggcttatg
     1921 atcctaccca aaccgtagcc ggcaaaactg gtacctgtat aggccaaaat gggtgggttg
     1981 gtttgttcac ttcctatgct ccgctggcta gtcctcgact cgcggtcgtt gtgattgctg
     2041 agggaagcga tggccgtaga cacttcccgg cagctgttgc cggcgaaatt tatcgcgagc
     2101 tcaatcatcg atttggtacg gctatcaata tgcaggttgt cgctgccgca gatgacgaag
     2161 agaaggaagt cggagattct gaggatggag atgacgcagc tctcgattcc actgcgacca
     2221 aggactcaac gaaagttacc gccgcatccg gcaatagcgt aactactgct cccataacca
     2281 caaaggccgc ttcaactgag actcaagcgc cgaagaatac ggttaagcga gtcctaatgc
     2341 ctttggaaaa gaaaactgtt gagccggcaa agaacaatcc agcattgcca actccaagta
     2401 atctccccga cggttcaaat acaactggca ccgaacaacg tcctcgtaga gcacatccca
     2461 atcagccgta agaaactcgt ccattaaact ccttttgaga tgcgccgtcg gcctaaagcc
     2521 aacggcgcaa ttttttatgt catctgagcc tttaccgcct tgacacatca cgataatttg
     2581 caggaatatt gcgcacgtta ttttcgacag agttttgggc acgattggag cgcatccccc
     2641 cttggatgca aaaaacagca accgctcctt gtgctcctag catcctaaac ttgccgcagg
     2701 cggacattgc aatgcttggt gttagcctac gccgcgtatg aaaaagaaga tttttggagg
     2761 ttcggacaac taatgaaaaa gacgattagg ttgatagctt taggtgcggt ggcggcaatg
     2821 tttgctttgc ccgttgcggc gaagagttca gttgcatccg ggttcgcatc tcaggaccag
     2881 tgcacagcag aaaataaaga cgcctggtac gcatccttca gagaagagcg aactaaggat
     2941 cagccaaagg catacgatgc tgccaagaaa tatctgggct gcgtcgcttc aagtagcgaa
     3001 gcacctactg atgcgcagaa ggcgattatc gattatctga agaagtggac gactgcttac
     3061 gaagagggaa ctagaaagaa caagctgcct cagttgatct ataccgacaa gaactatccc
     3121 gcagcctacg ctttagggaa agagctggtg gcccaggatc ctgaaaattt gaaagtcctt
     3181 atcgacctcg gcgcaaatgg ttatctcgct gctggtccaa agaacccgag cttgagtgca
     3241 gacgctttaa attacgcaaa gaaagccatt caacttctgg aatccggcaa gacagttgat
     3301 agttggcagc cactttctgg gaaggatgaa gcgcttgcat acctgcatta cacggttggc
     3361 tatctgacgc ttgaaaccga tccaagtggg gcactaggtc atttgatcaa agcggcccaa
     3421 actgaaacgc cgttgaagaa gtcgcccctg acctatggtt acatagcagg cgcttatgag
     3481 tcgggaccct atgcaaagca gtctgatgcg tacaaagctt gctgtgccgg gaaagaggaa
     3541 actcccgaga gcaaattgat gcttgcaaat atcaaccagg tcgtcgaccg catgattgat
     3601 gcttacgcgc gagctgtcgc tctggccggc tctgatgcat ccgtggctaa actcaagcct
     3661 gcctggaatg aaagtttgac gtcgtggtac aagttccggc ataacgactc gccggccggt
     3721 ttagaccaaa tggttgccgg aatcctttcg acgcctttac caccagagcc aaccccgatc
     3781 accacgttgc cagcttcaac tccggcagca actccgatcg gcagcacttc tggctcg
//
LOCUS       DASSFP010000041         4434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_304849, whole genome shotgun sequence.
ACCESSION   DASSFP010000041 DASSFP010000000
VERSION     DASSFP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304849"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFH91_00895"
     CDS             complement(<1..230)
                     /locus_tag="VFH91_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6861561.1"
                     /translation="MPNPATQMTLESLRSVPLFASLDDSAAIHLRNLLSEQEVPSNTR
                     LFNQGDQGNAMYLIESGRVRISIRDEEKQELTL"
     gene            complement(285..959)
                     /locus_tag="VFH91_00900"
     CDS             complement(285..959)
                     /locus_tag="VFH91_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6861562.1"
                     /translation="MQSILEARHVSRIYQMGANSVAALDDVSIEIKDAEFVAIQGTSG
                     SGKSTLLNLLGGLDHPSKGEVLFAAKQIGPFSKKEMARYRRFSVGMIFQNFNLIPTMT
                     ARENVALALAFGGMRGDERRKRSSELLGRVGLADRLQHRPSELSGGEQQRVAIARALA
                     NNPKVLLADEPTGNLDSIRAHELLGLLREMVNNDGLTIVMVTHDQELASSFAHRTIFM
                     KDGRVV"
     gene            complement(968..2890)
                     /locus_tag="VFH91_00905"
     CDS             complement(968..2890)
                     /locus_tag="VFH91_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HET6861563.1"
                     /translation="MGIRSKEFPPPFVYVFSKLAGANMRFREYHYRWEHDFKASPEQL
                     WPLVADTNRFNRDAGLPQLEFEKSRKRLRNARRKVRLSIHGMPVEWEEQPFEWVRPER
                     FGLERVYSRGPLARMRVLVELSRKDNGGTHMTYDVWATPRNMFGAVAIPLQIKLSNSP
                     KFNAAFKKYDSIALKGLEADITVDLSGDGKGDVGRLASLKQKLLADVPDDARNVVERL
                     IEFIQRSDDLSAGRIRPYELADKWNEPRRKVLETCLRATRIGLLDFQWNLLCPLCRGP
                     QHSSSSLKDIETNVHCQTCKIDFTVNFDRYVELTFRPNAAVRRVETAEFCVGSPERTP
                     HVVAQQLLPAAEGRTLNLPLEIGNYRLRALELEGSQLVNVSSEGEREVSIVVGPNGIT
                     GDQLSLSERSSLTIKNETDAEQLLILERMAWSDQATTAAEVTALQTFRDLFSTEALRP
                     GEQISVGTLTVLFTDLRNSTRLYREIGDATAFGRVMNHFDVLKQAIVSHEGAIVKTIG
                     DAVMAVFRCPADGVEAMLEVQEILASPPDGGIPLALKAGLHTGPCIAVTLNDRLDYFG
                     STVNMAARLEGLSCGLDVILSQNAFEDPKVQDLLRSEGYSAESFEMSLKGFEDERVEL
                     WRVRKNPGAQASSAAR"
     gene            complement(2946..3467)
                     /locus_tag="VFH91_00910"
     CDS             complement(2946..3467)
                     /locus_tag="VFH91_00910"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET6861564.1"
                     /translation="MSIISDVVESTKAIIQGMGVTLSYLPKRKWTVEYPDEPVTVQPR
                     YRGQHLLHVDELGREKCVACYLCAAACPSECIYIVAADDPRPAEERIGVDERYASVYN
                     IDYGRCIFCGYCVEACPKDAITHGYNFELSVYSRADLLKTKDDLLITKQVQSKNYRVA
                     FSHEDVDSEYKEV"
     gene            complement(3505..4287)
                     /locus_tag="VFH91_00915"
     CDS             complement(3505..4287)
                     /locus_tag="VFH91_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HET6861565.1"
                     /translation="MKRCPTCQRTFTDESLSFCTEDGTPLSPVEEGESTRVTADRSNQ
                     GSNAPPYQPPINTSQVPPRQSKNAPWILVIIVALVLGIVAGAIALVLLIPRMASRRQA
                     AIRPTANTNVRTTPSPSINTEAPKDENTKQAHEESQPPTDKDQVLADLTAIENEWTIA
                     NFNADKGKLDRILADDFVGSTTDGRVQGKVEYIDTIQRDSSVEKWNFEDLQLTLRGER
                     ATLSGKVRLMGGDGQQVLNFTDRFVWRDGRWQATSSEVSTAQ"
BASE COUNT         1089 a         1193 c         1047 g         1105 t
ORIGIN      
        1 agcgtcaatt cctgcttctc ttcatctcga atgctaatcc gcacacgacc actttcgatc
       61 agatacatag cattgccctg atcgccctga ttaaagaggc gtgtattgct cggcacttcc
      121 tgctcggaca gaagatttct caaatgtatt gccgcagagt catcgagaga agcaaagagc
      181 ggcactgagc gcaaagattc gagcgtcatt tgagtggcgg ggttaggcat atttcctccc
      241 taaaaggaat tacacaacaa ttagaatgaa agtgatcgtt cgcgctatac cactcttcca
      301 tccttcatga agatcgtccg gtgcgcaaag ctgcttgcca actcctgatc gtgggtaacc
      361 atcacgattg ttagaccatc gttattaacc atctctcgca acagaccgag caactcgtga
      421 gccctgatcg agtcgagatt accggtgggt tcgtcggcta aaagtacctt tggattgttg
      481 gccagtgcac gagcgatagc cacacgttgt tgttctccac ccgaaagctc cgagggccga
      541 tgctgcaacc gatccgcgag gccaactctt cctaacaact cactcgacct cttacgtctc
      601 tcatccccac gcattccgcc gaacgcaaga gccagcgcga cgttttcgcg cgccgtcatg
      661 gttggaatca agttgaagtt ttgaaagatc ataccaaccg aaaatcgccg gtaacgagcc
      721 atctccttct tgctgaatgg accaatctgt ttggcagcaa acaacacttc acccttgctg
      781 ggatgatcca gcccgccaag tagatttagt agagttgatt tacctgagcc tgaagtaccc
      841 tggattgcca caaactcggc gtctttgatt tcaatcgaca catcatcaag cgcggctacg
      901 ctgttggcgc ccatctgata gattcgcgaa acgtgtcgtg cttcgagtat cgactgcatt
      961 tatcgggtta tcgagctgcg ctggatgcct gagcgccagg attctttcga acgcgccaca
     1021 gttcgacgcg ttcatcctca aaacctttca aggacatttc aaagctttct gctgaatagc
     1081 cttcggagcg taaaagatcc tggacttttg ggtcttcaaa tgcgttttga gaaaggatga
     1141 catccaaacc gcatgacagg ccctcaaggc gcgcagccat gttgacagtg gagccaaaat
     1201 agtcgagtcg atcatttagc gtaacggcaa tgcatggacc agtatgcagg ccggccttca
     1261 acgctagcgg tatcccgcca tccggcggag aagcgaggat ctcctgcact tccaacatcg
     1321 cctcgacacc gtcggccgga cagcgaaaca ctgccattac cgcatcacca atcgtcttca
     1381 cgattgcgcc ctcgtgagac acgatcgcct gcttcagaac atcaaagtgg ttcatgacgc
     1441 gaccgaacgc tgtagcatca ccaatctctc gatagagcct cgtggagttt cgtagatcag
     1501 taaagagaac agtcagagtg ccaactgata tttgttcgcc cggacgcaac gcctcggtag
     1561 agaacagatc cctaaacgtt tgcagagccg ttacttcggc cgcagttgtc gcctgatcac
     1621 tccacgccat tcgttcgagt atcagtaact gctcagcatc ggtctcattc ttaatcgtga
     1681 gactggagcg ctcggacagg cttaattgat cgcccgtaat cccgtttggg ccaaccacaa
     1741 tcgatacctc gcgctctcct tcggacgaga cgttaaccag ttgtgatcct tccaactcga
     1801 gggctctcag gcggtagttg ccaatctcga gaggcaggtt aagtgttcga ccttcagcgg
     1861 ctggcagtag ctgctgtgcg acaacatgag gagtgcgttc gggactgcca acgcaaaact
     1921 ctgctgtctc aactcgccgg acggcagcat ttgggcgaaa ggttaattcc acatacctgt
     1981 cgaagttaac agtgaaatcg attttgcaag tttgacaatg gacgttagtc tcaatgtctt
     2041 ttaaagaaga gctggagtgt tgtggaccac gacaaagagg acataacaga ttccactgaa
     2101 aatccagtag tccaattctc gtagctcgca aacatgtttc cagtaccttt ctgcgcggct
     2161 cattccattt atcggcgagt tcgtaaggcc gaatacgacc ggcagagagg tcatcactcc
     2221 tttgaataaa ctctatcagc cgctcaacaa cgttacgagc atcgtccgga acgtcagcta
     2281 acaatttctg tttaagcgat gccagccggc cgacgtctcc cttgccatca ccggaaaggt
     2341 caactgtaat atctgcttcc agtcccttga gcgctatgga gtcgtatttc ttaaatgccg
     2401 cattgaactt tggtgaattg ctgagtttga tctgcagcgg tatggcaaca gcgccgaaca
     2461 tgttcctggg agtggcccat acgtcgtaag tcatatgtgt gccgccatta tctttgcgcg
     2521 acaactcaac gaggactctc atgcgggcca atggaccgcg cgagtaaact cgctcgagac
     2581 caaaacgctc aggtcgcacc cactcaaagg gctgttcttc ccactcgacg ggcattccat
     2641 gaattgacaa acggactttt cggcgagcat ttcgcagcct ttttctactt ttctcaaatt
     2701 caagttgcgg taatcctgca tcgcgattga accggttagt atcggccacg agcggccaca
     2761 actgttctgg actcgctttg aaatcgtgtt cccaacgata gtgatattcg cgaaacctca
     2821 tgttagcgcc cgcaagctta ctaaaaacat aaacgaatgg cggcgggaat tctttcgaac
     2881 gaatacccac cgccaagctc ttgatttaac aaccaaggag cacaacctaa cttgaaccta
     2941 actcgctaga cttccttata ctccgaatca acatcctcat gagagaaagc cacccggtag
     3001 ttctttgact gaacctgttt agtaattaac aaatcgtctt tggtcttcaa caagtcagcc
     3061 cggctataga ccgacaactc aaagttatag ccatgcgtaa tcgcatcctt gggacaggct
     3121 tcaacgcaat agccacagaa gatacagcgc ccatagtcaa tgttgtagac ggaggcataa
     3181 cgttcgtcca cgccaatgcg ttcttctgcg ggtcgtggat catcagccgc aacgatgtaa
     3241 atacattccg aaggacacgc agccgcacat agataacagg caacacattt ttcgcggccc
     3301 agttcatcga cgtgtagcaa gtgctgccca cgataacgag gttgaaccgt caccggctca
     3361 tcaggatact cgacagtcca ttttcgcttg ggcaggtacg aaagcgttac gcccattcct
     3421 tgaatgattg cttttgttga ttcaactaca tctgaaatga tcgacatgtt tgatcttgac
     3481 cgttttggtc taataatacc tttctcattg agcggtactg acttcggacg aagtagcttg
     3541 ccaccggcca tcgcgccaaa caaagcggtc ggtaaagtta agtacctgtt gtccgtctcc
     3601 gcccatcaat cgtactttgc ctgacagcgt ggcacgttca ccgcgcaacg tcaattgcag
     3661 gtcctcaaag ttccactttt caaccgatga gtcgcgttga attgtatcaa tatattcgac
     3721 ttttccctga acccgcccat cggtagttga gcctacgaag tcgtcggcca ggatccggtc
     3781 gagcttaccc ttatcagcgt tgaagtttgc aattgtccac tcgttctcga tagcagttag
     3841 atccgcgagc acctgatcct tatctgttgg tggctgactc tcctcgtgcg cctgcttggt
     3901 gttttcgtct tttggtgctt cagtatttat agacggactt ggagttgtgc gaacgtttgt
     3961 gtttgccgtc ggtctaatag ctgcttgtcg tcgactggcc atgcgaggaa ttaacaaaac
     4021 caacgcaatc gctccggcaa caatcccgag cactaaggcg actatgatca ccaagatcca
     4081 gggtgcgttt tttgactgtc gcggcggcac ctgactggta ttgataggag gttggtatgg
     4141 tggagcgttc gacccctgat tgctgcgatc tgcggtaacc cgggtggact cgccttcttc
     4201 tactggcgac agcggagttc cgtcttcagt acaaaaactt agactttcgt cagtaaaagt
     4261 tctttggcaa gtaggacaac gtttcatctc gaaattaaat caggagaaca ccagggacac
     4321 gatccagagc agaatcattc cgccgtagac aacgagactg acactcccgg ttacgatacc
     4381 aataatagcg aggggctttc cgccgtgagt gagcggttta tttttgatct gtga
//
LOCUS       DASSFP010000042         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_157758, whole genome shotgun sequence.
ACCESSION   DASSFP010000042 DASSFP010000000
VERSION     DASSFP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157758"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VFH91_00920"
     CDS             complement(<1..704)
                     /locus_tag="VFH91_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET6861566.1"
                     /translation="MRKARSLATLATVCALVIVFCASISAQNREKYVISAKAGGVNAV
                     TGQASVHGKGESEWQQLTVTDDLNAGDRVRTAGDGRVEILLNPGSYLRVGGNSEVELS
                     NNSLENLELRLIRGTAIVEATGADGLQLNISISTPHTKLAIVRHGLYRLNVVPGDATE
                     LIVRKGSVILNDSHTKVKGGNKVVFSATTVSVAKMTKEEKNVQEGVDNWSKDRGEALA
                     KANRRINGRMINSAFSA"
     gene            complement(835..1833)
                     /locus_tag="VFH91_00925"
     CDS             complement(835..1833)
                     /locus_tag="VFH91_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6861567.1"
                     /translation="MKYKVVGSKKLRWLVTLLFILPLLLIGPNARMQEPAEVIRIESD
                     LVDLKVSVIARNTSTPTPLLTQKDFTVREDGTPQEITFFAAADTPFDLVLLLDLSGSN
                     KKNLKMIRNSAKRFVEAARDVDRIALVAFTDQVWIYSPFTLDRAKLKKAIDEMDEAEG
                     GTNFWDSLDYVLRVLVHHNQETRRSAVVVMTDGVDNALPDVAGIGSQITFDRLLVQVE
                     NSDSVVFPIYLDTEEESVRRYHVPRAAYAIARQQLGQIAATCGTELYRASKLSDLDQL
                     YSRVIDDLRTVYSISYRPSNRAPDGKWRTVNVELPNRPELVARTKRGYYAKQFPSQ"
     gene            complement(1835..>2632)
                     /locus_tag="VFH91_00930"
     CDS             complement(1835..>2632)
                     /locus_tag="VFH91_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6861568.1"
                     /translation="DLRQSDFTLFEDGQQQQIVQFDSASAPFDMILLIDLSGSTKDVV
                     KLIRSAAQRFIDATRPADRIAVITFAGEAAVVSELTADRAILRQRVETIDTARGDTKL
                     YDSIDFTIKRVLSDNRKSRRTAIVLMSDGLDGTIPGVSGQQGSQHSYRETLRSIQEFD
                     GVLYSLWLNTEYQAFSPLDTQPEAFDMGHDRMKEMADAGGGVFYEVDKLADLAGAYEQ
                     VVADLGTVYSLAYRPSNSLRDGKWRAIRIAVGRSNAVARGKRGYYAN"
BASE COUNT          620 a          696 c          629 g          687 t
ORIGIN      
        1 gcggagaatg ctgaattgat catccgtcca tttatgcgac ggtttgcttt ggcgagcgcc
       61 tcacctcgat ccttgctcca gttatccacc ccttcctgaa cgtttttctc ttctttagtc
      121 atttttgcta ccgacacggt agtcgcgcta aaaacgactt tattgccgcc ttttaccttt
      181 gtgtgcgagt cgttcaggat cacactgccc tttcggacga ttaactccgt ggcgtctccc
      241 ggtactacgt tgaggcgata tagaccatgg cgaacgatcg ccagtttggt atgaggagta
      301 ctgatgctga tgttcagttg aagtccatct gcaccggttg cttcgacaat agccgtaccc
      361 cgaatcagcc tcaattccaa attttcaagt gaattattcg atagctcaac ttcagaattg
      421 ccgcccacac gcaaataaga gccagggtta agtagaattt cgacacgtcc atcgccagcc
      481 gttctcactc ggtcaccggc attcaagtcg tcggtcactg taagctgttg ccactccgat
      541 tcacctttac cgtgaaccga agcttgcccc gtgacggcat ttacgcctcc ggccttagct
      601 gaaatgacgt atttctcgcg attttgggct gagattgagg cacaaaagac aattacgaga
      661 gcacacacgg tcgccaacgt tgcgaggctt cgagcttttc tcatggaaag accctccaat
      721 ggaatgtggg atgcgccggt ggacatccgt cattatagac tcgtccctgt cttttacaag
      781 cttttcgtgg atcggatctc aaaattcaat tgtctcgtaa cgaatggacg tgagtcattg
      841 agagggaaac tgctttgcgt agtaacctct cttcgttcta gcgacgagct cgggtcgatt
      901 tggcaattcg acgttgaccg ttcgccattt gccgtcgggc gctctattgg aaggacggta
      961 actgatgctg taaaccgtac gcagatcgtc aataactcgg gaatacagtt ggtccagatc
     1021 gcttaatttt gacgcacggt ataattctgt accgcaggtt gcggctattt ggccaagctg
     1081 ttgccgggca atagcgtaag cggctcgtgg tacgtgatag cgccttacgc tttcctcctc
     1141 ggtgtccaaa tagatcggaa acaccacgct gtcagaattc tcaacttgaa ccagcaaacg
     1201 atcgaaggtg atttgagagc ctatgccggc aacgtccggc agtgcattat cgactccatc
     1261 cgtcatcacc actaccgctg aacgtcgcgt ctcctggtta tgatggacca gaactcgcag
     1321 gacatagtcg agagaatccc aaaaatttgt tcctccctct gcttcatcca tttcatcgat
     1381 cgccttcttc aacttcgctc ggtccagcgt gaatggtgag tagatccaga cttggtcggt
     1441 gaacgccact agcgcgattc gatcgacatc tcgtgcggcc tcgacaaacc gtttcgccga
     1501 gttacgaatc atctttagat tctttttatt tgacccggag agatcgagca aaaggaccag
     1561 gtcgaaaggg gtgtccgctg cggcaaagaa ggtgatctcc tgcggcgtcc cgtcttcgcg
     1621 tacggtaaag tctttttgag ttagtagcgg agtaggagtg ctggtgttgc gggcgataac
     1681 actcaccttc agatcaacaa gatctgattc gatgcgtata acttcggccg gttcctgcat
     1741 ccgggcattc ggtcctatga gcaacagagg caaaatgaac aacaaggtca caagccaccg
     1801 aagctttttt gagccaacca ctttgtattt catgttagtt cgcgtaatag ccacgcttgc
     1861 cgcgcgctac tgcgttggaa cgacccaccg caattctaat cgctcgccat tttccgtccc
     1921 tcaaactgtt agagggtcga taggctaaac tgtagacggt tcctaaatcc gctaccacct
     1981 gttcataggc accagccaga tcggctagtt tatctacttc ataaaacaca ccaccaccgg
     2041 cgtcggccat ttccttcatc cgatcatgac ccatatcaaa cgcctcaggt tgagtatcga
     2101 gaggactgaa agcctgatat tcagtattta gccacaacga gtaaaggacc ccatcgaact
     2161 cttgaatact gcgcagcgtt tccctgtaag agtgctgcga accctgttga ccgcttacac
     2221 cgggaattgt tccatccaag ccatcactca ttaaaacaat cgctgtacgt cgtgacttcc
     2281 tgttatcgct caacacgcgt ttgattgtga agtctatcga gtcataaagt ttggtatcac
     2341 cacgggctgt gtcgatcgtc tcgactcttt gccgcagaat ggctcgatcg gcagttaatt
     2401 cggacacgac ggccgcctct ccggcaaaag tgattacggc aatccggtca gcaggtcgag
     2461 tagcgtcgat gaatcgttga gcagcagaac gaatgagctt gacgacgtct tttgtagatc
     2521 ctgacaagtc gatcaggaga atcatatcga atggcgctga ggccgagtcg aattgcacaa
     2581 tttgttgctg ttgaccgtct tcaaacaacg taaagtctga ttgacgcaag tc
//
LOCUS       DASSFP010000043         5064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_9395, whole genome shotgun sequence.
ACCESSION   DASSFP010000043 DASSFP010000000
VERSION     DASSFP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9395"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VFH91_00935"
     CDS             <1..1545
                     /locus_tag="VFH91_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6861569.1"
                     /translation="YDPIRDVRTACYAWADNKELDISELPPMPITTSGPNSRALRLAQ
                     VIITNDYIKAIRGHPSVIVGPDDGLRTQSSMAIPMSVMGRIIGTVEVQSYKSDAYRRQ
                     HATAMAMAANLTAVAIENIRLLKLERSAKESAEESNRLKDEFLATVSHELRTPLTAIL
                     GWSRLLESGALSGPAELQAIDTIWRNAKAQAQIIDDILDVSRIITGNLYMDLHPVDLG
                     PVIENAVNVVRPTADAKSIKIECELSRIAAVVSGDANRLQQVVWNLLSNAVKFTNSGG
                     RVNVKLSQENASVMVSVSDTGQGIRREFLPYVFDRFRQADSTTTRQHGGLGLGLAIAR
                     HLIEIHGGTITAESEGAGKGATFVITLPWMESATNQAEFQTAGNVRPTKAPMLLAGVC
                     VLVVDDDEDTLQLMATALTSRQAKVVAVSSAGEAIRAIEVNRPDVLISDIAMPGEDGY
                     GLIERIRTMEQDYAASIPAVAITAYAKEEDRERALSSGFQLYLAKPVELTELISVVAQ
                     AARREF"
     gene            1577..2377
                     /locus_tag="VFH91_00940"
     CDS             1577..2377
                     /locus_tag="VFH91_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6861570.1"
                     /translation="MPKVTIEDIEMAYTDVGSGPAVVFIHGYPFNRSLWTEQVTALAG
                     THRLVVPDLRGFGESDCGNLPSTMNRLALDINLLLDHLRIQKAIIAGLSMGGYVTLAF
                     YKEFQDRVSGLLLANTRAEQDSFEAKSSRREQAEKALTEGMAGIADSMLPKLLTPETV
                     SKKPDLVKRIRDMMLKTDPKGAAAALLGMAGREDFTSQLSQISVPVLIIAGRDDALIP
                     MQHSERMHQAIASSRLAVIEHAAHVVNLEQAEAFNHEMMQFVTQLNSW"
     gene            2386..2826
                     /locus_tag="VFH91_00945"
     CDS             2386..2826
                     /locus_tag="VFH91_00945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET6861571.1"
                     /translation="MASHQVFTIEERVRWGDVDAARIIFYGAYIHFFELAETEFFRKV
                     GLAYGQMFDELGIWLPRVHLECDFHKVARLDDLLQVSVYVGRFGSKSMKLNFEVRRLS
                     DEALVATAHFILAAIKQDTFETVPIPTELRERLAPYNANEPAND"
     gene            2819..3451
                     /gene="pdxH"
                     /locus_tag="VFH91_00950"
     CDS             2819..3451
                     /gene="pdxH"
                     /locus_tag="VFH91_00950"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET6861572.1"
                     /translation="MIDPLHNPDEETLDEASIDRDPITLFDRWFKDAQTANLLLPEAM
                     TLATVNSDGRPTARMVLLKQVDHDGFVFFTNYSSSKASQLDENDAAALVFHWAPVERQ
                     VRVEGRVVKTSADESRAYFQTRPRESQIGAWASPQSEVIESRSILEQRKAQLEEKYRD
                     RPIDCPPNWGGYRLIPDRIEFWKGRIGRLHDRILYSRTAEGGWTIQRLAP"
     gene            complement(3459..4655)
                     /locus_tag="VFH91_00955"
     CDS             complement(3459..4655)
                     /locus_tag="VFH91_00955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6861573.1"
                     /translation="MNEFDLLIVGAGPAGAYAAELLAQKGARVALFNGQPLGKPKACG
                     GGVTTKALKAWPQLLNAVGRTIYELDLYSPAGKRLHLKLEQPFAVYSRLAFDNYLRER
                     ARLAGATLFDEKVSIRKARNNGAGWMLKSENGAEWRGRTLVGADGANSAVARLMVGPL
                     PPSDMEVAFGYRAPLPEHNLASTVVAFLPNWVGYAWAFPRPDHISFGIATTQDAFEHQ
                     PLDDLLWDFMVGYYKYPGGSTVNFWNGDGDSRIKEHLSATAERYAARIPGLSDISWER
                     RRNSGDGWALLGDAAGFADPVTGEGIYYALRSAQLFAESYLSGHPLDYESRWKADFGL
                     ELRRAAQMRQRFYGNFWGAPFTERMIDFARGHRGVKSVLADLVAGEQGYVDLKRKLLK
                     SAFRPI"
     gene            4804..>5064
                     /locus_tag="VFH91_00960"
     CDS             4804..>5064
                     /locus_tag="VFH91_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861574.1"
                     /translation="MMSNPGSIVFSGLAPHPPIMVPEVGQESVKDVRQSIDGMAELTR
                     RVLESGAETVVLISPHAPLEADTFVAYAEPTLFGDFTRFRAPQ"
BASE COUNT         1256 a         1276 c         1332 g         1200 t
ORIGIN      
        1 tacgatccga ttcgagatgt gcgaactgct tgttatgcct gggccgacaa taaagaatta
       61 gatatctcgg aactcccacc gatgccaatc acgacttcgg gcccaaacag ccgagcgttg
      121 cgcctggccc aggtaatcat caccaacgac tacataaagg caatacgtgg ccatccttcc
      181 gtaatcgtcg gacctgacga tggattacga acacaatcgt cgatggcgat tccgatgtct
      241 gtgatggggc gaatcatcgg aacagttgag gttcaaagtt ataagagcga cgcttatcgc
      301 cggcagcatg cgactgcaat ggcgatggcc gccaatctga ctgccgtcgc gattgaaaat
      361 attcggttac tcaagctgga acgttcggca aaggaatcgg cggaggaatc gaatcggctc
      421 aaagacgaat ttctggcgac ggtttctcac gaattacgta cgccgctgac ggcaattctc
      481 ggttggtcca gattgcttga aagtggagca ttgtctggac cagcggaact tcaggcaatc
      541 gacacaatct ggcgcaacgc caaagctcag gcccagatta ttgatgacat acttgacgtc
      601 tctcgaatta tcaccggcaa cctgtacatg gatctgcacc ctgtcgattt gggcccagta
      661 attgagaatg cggtaaatgt ggtgcggccc acggcagatg ctaagagcat taagatcgag
      721 tgcgagctca gtcgcattgc agcggtagtt tctggtgacg caaacagatt gcagcaagta
      781 gtttggaatc tgctgtcgaa cgctgtgaag tttacgaaca gcggaggtcg agtaaacgtt
      841 aagctatctc aagagaacgc atctgtgatg gtcagcgttt ccgatactgg ccagggcatc
      901 aggcgtgagt ttcttcctta tgtattcgat cggttcagac aggcagatag cacaactaca
      961 cgacaacatg gcggtctcgg tctcggactg gccatagcgc gacatctgat cgaaatacat
     1021 ggcggcacaa tcactgccga aagcgaaggt gccggaaagg gtgcgacgtt tgtcatcacg
     1081 ctaccatgga tggagtcggc aactaaccag gcggaatttc agacagcggg aaatgtgcgg
     1141 ccgacaaagg ccccaatgct cttggccggt gtttgtgttc ttgtagtgga cgacgatgaa
     1201 gacacgctgc agctcatggc caccgcgttg accagccggc aggctaaagt ggtagccgtt
     1261 tcttctgctg gagaggcaat cagagctata gaagtcaatc gaccggatgt actcatctcg
     1321 gatattgcaa tgccgggtga ggacggttac ggtctgatcg agcgaatacg aacgatggaa
     1381 caagattacg ccgcttcgat tccggcggtc gctattactg catacgccaa ggaagaagat
     1441 cgcgaacgag cgctgtcgtc gggctttcag ctttatctgg cgaaacctgt cgagctaact
     1501 gaactaatat cggttgtggc gcaagcggcc cgccgggagt tttgaatttc cagtaaactc
     1561 gggcacttta ttacatatgc cgaaagtaac gattgaagat atcgagatgg cctacacgga
     1621 cgtggggtca ggtccagcag tcgtgtttat tcatggctat cctttcaatc gttcgctttg
     1681 gaccgaacaa gtaaccgcac tcgcgggaac tcacagatta gtcgtgcccg atctgagagg
     1741 gtttggcgag agcgattgtg gaaacttgcc ctcgaccatg aatcgattgg cgctcgatat
     1801 taatcttctg ttggatcact tgcgaatcca aaaagcgatc attgccggct tatcgatggg
     1861 tggctacgtt accctcgcgt tttacaaaga atttcaggac cgggtcagtg ggctactgct
     1921 tgccaatact cgcgctgaac aagatagctt tgaagcaaag agctctcgtc gtgagcaggc
     1981 tgagaaagcc ttgacggaag ggatggctgg cattgccgac tcgatgttgc caaaacttct
     2041 gacaccagag acggtgagca agaagcccga cctcgttaaa cgaattcgtg acatgatgct
     2101 aaagacagat ccaaagggtg ctgctgctgc actcctgggc atggcgggtc gggaggactt
     2161 tacaagtcag ctgtctcaga tttcagttcc ggttcttatt atcgctggac gagacgacgc
     2221 acttatcccg atgcaacaca gcgagaggat gcaccaagcg atagcaagtt ctcggcttgc
     2281 tgtgattgaa catgccgctc atgttgtaaa cctcgaacag gcagaagcct tcaatcacga
     2341 gatgatgcag ttcgttactc aactcaattc ctggtaggtt ccattttggc aagtcatcaa
     2401 gtctttacga tcgaggaacg tgtgcgatgg ggagatgtcg atgccgcacg aatcattttt
     2461 tacggcgcat atattcattt ctttgagctc gccgagaccg agttttttcg caaagtggga
     2521 cttgcgtacg gtcaaatgtt tgatgagtta ggcatttggt tgccgcgagt gcatctcgag
     2581 tgcgactttc ataaagtagc gcgcctggat gatctgcttc aggtcagcgt ttacgtcggc
     2641 cggtttggct ccaaatcaat gaagttgaat tttgaggtca gacggcttag cgatgaagct
     2701 ttggttgcta ccgctcactt catacttgct gcgataaaac aagatacgtt tgaaactgtt
     2761 ccaatcccaa ccgagttgcg agaaaggctc gctccctaca acgcaaacga acccgcaaat
     2821 gattgatccg ttgcacaatc cagatgaaga aacgctcgac gaggcgtcta tcgatcgcga
     2881 tcctatcacc ctcttcgatc gctggtttaa ggatgctcaa acagccaacc ttctactgcc
     2941 tgaggcaatg acgttagcaa ccgttaactc tgatggtagg ccaacggcgc ggatggtctt
     3001 gttgaaacag gtagaccacg acggctttgt gttttttaca aattacagca gctcaaaagc
     3061 gagtcaactc gatgagaacg acgccgccgc tcttgttttt cattgggccc ccgtagaacg
     3121 acaggtaaga gtagaaggac gcgtggtgaa aacgtcagct gatgaatcca gggcttattt
     3181 tcagactcga ccgagggaga gtcagatcgg tgcgtgggca tctccgcaaa gtgaggtcat
     3241 tgagagtcgt tccatacttg agcaacgaaa ggcgcaactt gaggagaaat accgagatcg
     3301 cccgattgat tgtcctccca actggggtgg ttatcgcttg ataccagata ggattgaatt
     3361 ttggaaaggt cgcatcggtc gtttgcacga tcggattctt tatagtcgaa ccgctgaagg
     3421 tggatggaca attcagcgcc tcgctccctg acttcaattt agattggccg aaaagcactc
     3481 ttcaataact tccgcttcaa gtcgacataa ccctgttcac ccgccaccag atcagccagt
     3541 acacttttaa caccgcgatg tccgcgggcg aagtcgatca tccgttcagt aaacggtgct
     3601 ccccagaaat taccgtagaa gcgttgccgc atttgagccg ctcttcgcag ctcgagccca
     3661 aagtctgcct tccaacgact ctcgtagtcg agtgggtggc cgctcaaata gctttcagca
     3721 aatagttggg ccgagcgtaa cgcgtaataa atgccttcgc ctgtaacggg atcggcaaag
     3781 ccggcggcat cgccaagtaa ggcccatcca tcaccgctgt ttcttctgcg ctcccaacta
     3841 atgtcggaaa gtccgggaat gcgcgccgca tagcgttcgg cagttgcgga cagatgttcc
     3901 ttaatccttg agtcaccgtc cccattccaa aaattgactg ttgagccgcc aggatacttg
     3961 taataaccaa ccataaaatc ccaaagcaaa tcatcgagcg gttgatgttc aaacgcgtcc
     4021 tgcgttgtcg cgattccaaa tgaaatgtga tccggccgtg gaaaagccca tgcgtaaccc
     4081 acccaattgg gcagaaacgc gacaacagtt gaggcaaggt tgtgctcggg aagtggggcc
     4141 cggtaaccga aagcaacttc catatcggac ggcggcaatg ggccgaccat caacctcgca
     4201 acggcgctgt tcgctccatc ggctccgact aatgtccttc ctcgccattc ggccccattt
     4261 tcgcttttga gcatccagcc tgcaccattg tttcttgctt tacgtatgga cactttctcg
     4321 tcaaacagtg tggcccccgc tagtcgagcc ctttctcgta aatagttatc gaatgccagt
     4381 cgtgaataga cggcgaatgg ctgttcgagt tttaagtgca gacgttttcc ggcgggcgaa
     4441 tataggtcca actcatagat cgtacggccg acggcattga gcagttgtgg ccaggccttt
     4501 aacgctttgg tcgtaactcc gcccccacaa gccttgggct tacccagggg ctgaccgttg
     4561 aagagagcta cccttgctcc cttttgcgca agtaactcag cagcataggc ccctgcaggg
     4621 ccagcaccca cgattagtag atcaaactcg ttcattcgtt ttaataagca acaccaaatt
     4681 ctaaccacat catagaggtt ttcttcttca gatcaattgt ggcgacagag acttggcgtt
     4741 agaatggcca acgttcccac gaaaaattca aaccccgttg gtctcagctg accgaagtta
     4801 aagatgatga gtaacccagg ctccatagtc ttttcaggac tagctccgca tccacctatt
     4861 atggtgccgg aagtaggtca ggagtcggtc aaagacgtgc gacagtcgat agacgggatg
     4921 gctgagttaa cgagacgcgt cctggagagc ggcgcagaga cggtggttct gatatctcct
     4981 catgctccgc tcgaagctga cacttttgtt gcttatgcag aaccgacctt gtttggtgac
     5041 ttcacgagat ttagagcacc gcaa
//
LOCUS       DASSFP010000044         5920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_754491, whole genome shotgun sequence.
ACCESSION   DASSFP010000044 DASSFP010000000
VERSION     DASSFP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754491"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="VFH91_00965"
     CDS             complement(<1..1599)
                     /locus_tag="VFH91_00965"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6861575.1"
                     /translation="MSIGSLKFVASLMSAGLLMSSFSYRTAGTTAVRPNFTIQQTDQA
                     DVALREGRRLLKRGRSDQALIQLRNALNLYTNAKNQSGVAAAHNELGDLYLRQGQYPV
                     ALDHYQKALDGFLGVDKKNDVVNSAVGIGDDKFNANLMLAKIGDVNFRLGRTSEAMAA
                     YGRMVVKKPESAGSKVTRRFGGLGGIMGGISSGNVSVAAPTSALTVALEMKKELDEYR
                     VSIVYASYELGMGRLAYASNDLDSAKKHFENALEGAGSSLAGIAKLGQVQRFRAAART
                     SLGDVALRQSKWKDAGKYYADAKKGAQDDKRLDLIWPAQRGIGRTFWLQAAQEKDPKK
                     AMSLRESAVSNYRESIGTIETLRQGSLRADEARTTFLATMKDVFDEAASAYAEMALIT
                     SSNSTSPLTAKALEYAGEAFKINEQSRSRSLLDLLNETGGAITEGVPADLLKRKQDNL
                     DRQQDIADVLTGVNISNEELKKKPSELDEELAKLQGEYEEIENQIRTASPRYAALTSN
                     QALTLSDVQQKVLDDRTLLLEYALQ"
     gene            complement(1690..2571)
                     /locus_tag="VFH91_00970"
     CDS             complement(1690..2571)
                     /locus_tag="VFH91_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HET6861576.1"
                     /translation="MKIAFLAFSLICVMVAVNAAVPQDQEDVRGAFLTSRPKDKPQTQ
                     TAPAKANRRRPKTTTPVTNTGGTKVTEPSNSSPDKPVVVNKARLGLGLTLFTRDSNGL
                     AVRTDPSRVFRKGDRVRVLLETNTDGFLYIFNTTNDGPPIMIYPDVNLDEAGNYLQAH
                     VPFEIPSSGAPQERLRWLVFDENAGDEKLYFVFTREPLNGIPLEDDLVSFCKASSSAC
                     PLRPAAELWELVKKEMKQPLQTDQTPRYGKAQTAYETQASTRGLGLAKDDPQPSVIMM
                     ASSTSSTLVTTLDLVHQ"
     gene            complement(2698..4665)
                     /locus_tag="VFH91_00975"
     CDS             complement(2698..4665)
                     /locus_tag="VFH91_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6861577.1"
                     /translation="MKYCAACKEKYDDSVSFCPVDGEVLEDDPSSLVNTVLDGQYQME
                     ALLGKGGMGAVYQARHILLGDRVAIKVLPPEVRSNAEWLRRFRREGQAARRFRHQNAV
                     TVYDLRTAADGTIYMVMEYVEGHTLDAEIKKRGRFIPREALEIMTPIMSVLDTAHAMG
                     VVHRDLKPENIMVGNPTGNGAPVIKLLDLGIAKMREIAGVDTAGNTALTTAGQVLGTP
                     YYMSPEQWGEIPRDGNQEIDGRADIYSLGLVFYEMIAGRRCFTGNTLLELRREHISTK
                     PKPLIEVVPNVPKAFSDAIERATAKDRGDRQATAAVLAQELSSSLASVSSDKHVTDRL
                     REIEDADTVALPRALQTKSDTNAPTIITLDPLATLPPPLSEPLASTTPSIATEVPKAS
                     NTVVESGLEQSSATVPQVPIPRPVVEAPARKSRAAGLIIGLGAVLVILFVVVAGGIYL
                     WSRSRATSPPVTTSETNLPAAPVARDVSRYWLELERTSGASSRVAGLVPIASGQSFKM
                     HFAFKDDGYLYIVGPGNNNKPTVFLSAQPLPASGVQTNKVIRDQEFSFPNGTGNNLTL
                     DNRPGTDNFRIVFSKTPLTSPAFFNDKVTGEPLNATQQTELDAFLSKYQQKSPASELD
                     ESNRNAPFVRVKVVGTEISDPLVFDVRIQHN"
     gene            complement(4764..>5920)
                     /gene="glgP"
                     /locus_tag="VFH91_00980"
     CDS             complement(4764..>5920)
                     /gene="glgP"
                     /locus_tag="VFH91_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET6861578.1"
                     /translation="GLVMRCFGETYLQQLGLTQDQFLGLGKVDPNNSHEHYGLTPLAI
                     RMARSTNGVSLKHGEVSRALWQKLWPGKKLAEIPITHITNGVHAPTWISPLLRFLFEK
                     YVGEDWHEQLRDRRRWKDSVGRIPDEELWIAHSLLKERLIAFVRYRSVLSRKAAGERA
                     DYVEAARTVLDPRALTIGFARRVAGYKRWNLVLSDEERLLRLIDNEERPVQFVFAGKA
                     HPQDEGSKRVLQQLAQWKYDLRVRNRAVFLEDYDQEIARQLVQSVDVWLNVPRRPLEA
                     SGTSGEKVAINGGLNLSILDGWWLEAYDGSNGFAIGNGDDSTDVGLIDAADAESLYRV
                     LEEEVVPVYYDRDSAGLPHRWVTMMKRAIETLAPRFNSDRMVEDYARKIY"
BASE COUNT         1342 a         1612 c         1460 g         1506 t
ORIGIN      
        1 ttgcaaagcg tattcaagga gcagcgtgcg atcatcaagg actttctgct gaacgtcgct
       61 caatgtcaga gcttgattcg aggtcagagc tgcgtaacgc gggctggcag tacgaatttg
      121 attttcaatc tcttcgtatt ccccctgcag ttttgcgagc tcctcgtcca actctgatgg
      181 tttcttcttt aactcctcgt tggagatgtt gacaccagtt aggacatcag caatgtcttg
      241 ctgccgatcg agattgtctt gctttcgctt caacagatca gcgggtacgc cttctgttat
      301 cgctccacct gtttcattca acagatcgag cagcgatcga gaacgagatt gttcattaat
      361 cttgaacgct tcaccggcat attcgagcgc ctttgccgtt aatggggaag tcgaattgga
      421 acttgtgatc agcgccattt cggcgtaagc acttgcagct tcgtcaaaaa catctttcat
      481 ggttgccaga aaggttgtgc gcgcttcatc cgctcgcaaa cttccctgac ggagcgtctc
      541 gattgtgcca atcgactcgc gatagttact tacagcagac tcgcgcagag acatagcttt
      601 cttcggatct ttttcctggg cagcctgaag ccaaaatgtc cggccaattc cgcgttgggc
      661 cggccagata agatccaatc gcttatcgtc ctgggctcct ttctttgcgt ctgcgtaata
      721 cttgcctgcg tctttccact tgctttgacg caacgcgacg tcacctaagc ttgtgcgtgc
      781 cgccgcgcgg aaacgctgca cctggcccaa cttcgcaatt cccgcaaggc tgctgcctgc
      841 accttcgaga gcattctcaa aatgcttctt cgcgctatct aaatcattgc tggcataggc
      901 caggcgaccc atccctagtt catagctggc ataaacgatg gagactctgt actcatcgag
      961 ctctttcttc atttcaagcg cgaccgtcaa ggcacttgtg ggcgccgcga cagacacatt
     1021 tcccgatgag atgccaccca taattccgcc gagtccaccg aagcgtcggg taacttttga
     1081 gcctgcactc tcaggcttct tcacaaccat tcgtccgtaa gcggccatgg cttcagaggt
     1141 tcggcccagg cggaaattaa catcaccgat tttcgcaagc atcaagttcg cattgaactt
     1201 atcgtctcca attcctaccg cggaattgac gacgtcgttc ttcttgtcga ctccgagaaa
     1261 accgtcgagc gctttctggt aatggtccag cgcgacgggg tattgacctt gtcggagata
     1321 caagtcgccc agttcgttat gagcggccgc tacaccgctt tgattttttg cgttggtgta
     1381 aagattgagc gcgtttcgca gttgaatgag cgcctgatcc gagcgaccac gcttcagtag
     1441 tcgccgtcct tctcgaagcg caacatctgc ctggtcggtt tgttgaattg taaagttcgg
     1501 tcgaacagcc gttgttcccg ctgtcctata gctgaacgaa ctcatcagaa gtcctgcact
     1561 cattaacgac gcgacaaatt tcaaacttcc aatgctcatc tcgtctcccc ttttcaattc
     1621 tcaggcatta accatcctcc acatttcgcg agtgctaggc ttgcgaataa tgtgttggac
     1681 caataactat tactggtgaa ccaaatccag agtggttacc agggttgaac ttgttgacga
     1741 agccatcatg atgactgaag gttgcggatc gtccttagcg agacccaatc ctcgagttga
     1801 tgcctgggtt tcataagcgg tctgcgcctt cccgtaacgc ggagtctgat ccgtctgcaa
     1861 cggttgtttc atctcttttt tgaccaattc ccaaagctcg gcagcgggcc gcaaagggca
     1921 agcggatgag gacgccttac aaaaactgac cagatcgtct tcaagaggta ttccattcag
     1981 tggctcgcgt gtgaagacaa aataaagttt ttcatcaccc gcattctcgt caaacacgag
     2041 ccagcggagt cgttcctggg gcgcaccact agaggggatc tcgaatggca cgtgagcttg
     2101 aagatagttg cccgcttcat cgaggtttac atcgggatag atcatgatgg gtggaccatc
     2161 gttcgtcgtg ttgaagatgt aaagaaaacc gtctgtgttc gtctcaagca ggactcggac
     2221 ccgatcaccc ttgcgaaata cccgggaagg atctgttctt actgctagtc catttgagtc
     2281 gcgcgtgaac agcgtcaatc cgagaccaag gcgcgccttg ttgacgacca ccggcttgtc
     2341 aggactcgaa ttcgaaggtt cggttacttt ggtaccacca gtgtttgtta ccggggtagt
     2401 cgtttttggc ctgcgccgat ttgcttttgc gggtgcggtc tgtgtttgag gcttatcctt
     2461 cggtctggag gtgagaaaag ctccgcggac gtcttcctga tcctgaggca ccgcggcgtt
     2521 tacagccacc ataacgcata tcagactaaa ggcaagaaat gcgattttca ttgtttactt
     2581 ctccttattg cgcaaccgaa tactctgagt cccgggaggt cagattcaga attccggttt
     2641 gaagaggaca gcgaagggca gtaaagctca tgttccgtcc gagattgcgt acgtggtcta
     2701 gttatgttga atgcgaacat cgaagacgag cgggtcgcta atttcagtcc ccactacctt
     2761 aacgcgtaca aagggtgcat ttcggttcga ttcatctaac tcgcttgccg gcgatttctg
     2821 ctggtactta cttaagaacg catctaactc agtttgttgc gtcgcgttca aaggttctcc
     2881 agtaactttg tcattgaaga aagccggact ggtcaaaggt gttttcgaaa aaacaattct
     2941 aaagttgtca gtgccaggcc gattatcgag tgtcagatta tttccggttc cattcggaaa
     3001 actaaactcc tggtcgcgga taaccttatt ggtttgaaca cccgaagccg gaagtggctg
     3061 cgcacttaaa aagacggtgg gcttattgtt gttgcccggc ccgacaatat agagataccc
     3121 atcgtcctta aacgcaaaat gcattttgaa cgactggccg gacgcgattg gcactaatcc
     3181 agccacgcgc gacgacgctc ctgaagtcct ttcaagttcg agccaataac gactcacatc
     3241 acgagcaacc ggagcagccg gcaggttagt ctcgctggta gtaactggag gactagttgc
     3301 acgggatcta ctccacaagt aaatgccacc ggcaacgaca acaaacaaga taacgagtac
     3361 tgcgcccaaa ccgattatca atccggcggc tcgactcttc ctggcaggtg cctcaaccac
     3421 tgggcgcggt atcgggactt gcggaacagt ggcactcgat tgctctaatc ccgattcaac
     3481 gacagtgttt gaagctttcg gaacctcagt agcaatggac ggcgtcgtac tggccagcgg
     3541 ttcagaaagc ggaggtggca gggtcgctaa cggatcaagt gtaataattg tcggtgcgtt
     3601 cgtgtccgac tttgtttgta gtgccctggg taatgcaaca gtatctgcgt cttcaatctc
     3661 acgaagccga tccgttacgt gcttgtcact ggagacagac gcgagtgacg aacttagttc
     3721 ctgggccaac actgcggctg tggcctgacg atctcctcgg tccttggcgg tcgcccgttc
     3781 gatcgcgtcg ctgaaagcct tcggcacatt tgggactact tcaatcagcg gtttcggttt
     3841 ggtcgaaata tgctcgcgtc gcagttcgag aagtgtgttg ccggtaaaac aacggcgccc
     3901 ggcgatcatc tcgtaaaaga ccagcccgag gctgtatatg tcggcacgtc catcaatctc
     3961 ctgattgccg tcacgaggta tctcgcccca ctgctcaggg gacatgtagt aaggcgttcc
     4021 caagacctgt ccggcagtag tcagggctgt attgccagcc gtatcgacac cggcgatttc
     4081 tctcatcttt gctatcccaa ggtccagaag cttaataact ggcgctccat taccagtggg
     4141 atttccgacc ataatgtttt caggttttaa atcacggtga acaaccccca tggcgtgcgc
     4201 ggtgtcgagc acactcatga tcggggtcat gatctcgaga gcttcgcgag gaatgaaacg
     4261 gccgcgcttc ttaatttcag cgtcaagcgt atggccctcg acatactcca tcaccatgta
     4321 aatggttccg tccgcagccg ttcgtaaatc gtaaacagtg acggcatttt ggtgacgaaa
     4381 gcggcgagcg gcctgacctt ccctgcgaaa ccgccgtagc cattcagcat tggaacgaac
     4441 ttcaggcggc agaactttga tggcgacccg gtctccgagc agtatgtggc gggcctgata
     4501 caccgcgccc attccacctt tgcccagcaa ggcttccatc tggtattgac cgtcgagaac
     4561 ggtgttcacc aacgaggagg gatcgtcttc gagtacctcg ccgtcgacgg ggcaaaagct
     4621 tacgctgtca tcgtattttt ctttgcaggc ggcgcaatat ttcattcggg gctaaaacca
     4681 acctggcatt taggacggtt gagtagtcta gcattggttg aaggtagaac tataggactt
     4741 atatgaccaa taggacctat accttaatag atctttcgag cgtagtcttc aaccattcga
     4801 tcgctattaa accgaggtgc aagagtctcg atcgctcgct tcatcattgt cacccaacga
     4861 tgcggaagtc ccgcgctgtc ccggtcataa taaacaggga caacctcctc ctccagcacc
     4921 cggtaaagag actcggcatc ggcggcatca atgagcccaa cgtccgtgct gtcgtcacca
     4981 tttccgatgg caaaaccgtt cgatccgtca taggcttcga gccaccatcc atcaaggatc
     5041 gacaggttaa gacccccatt gatggccact ttctctccac tggtaccgga cgcttctaaa
     5101 ggccttcgtg ggacgttcaa ccaaacatct actgactgca ccaactgcct tgcgatctcc
     5161 tggtcatagt cttcgaggaa aactgcccgg ttgcgcacac gcaagtcata cttccattga
     5221 gccagttgct gtaacactcg tttcgagcct tcatcctgcg gatgtgcctt gcccgcaaac
     5281 acaaactgta ccggtctctc ttcattgtcg atcaagcgga gcagccgttc ctcatccgaa
     5341 aggaccaggt tccaacgttt gtaacctgca acccttcgcg caaagccaat ggttaatgcc
     5401 cgtggatcga ggactgtgcg tgcggcctct acgtaatctg ctcgctcacc cgcagccttt
     5461 ctcgaaagca ccgagcgata acgcacaaat gctattagac gctccttcag cagtgaatgc
     5521 gcaatccata attcctcatc tggaattctc ccaaccgagt ctttccatcg acgacggtcc
     5581 cttagttgct cgtgccagtc ctcgcctaca tatttttcaa acagaaacct caacaatggt
     5641 gaaatccacg ttggagcatg cactccattt gtgatgtgcg tgatcggaat ttctgcgagt
     5701 ttttttccgg gccacagctt ctgccacaac gctcgcgaca cctcgccgtg tttcagactc
     5761 acaccgttgg ttgaacgggc catgcgaatt gccagtggtg ttagtccgta gtgttcatga
     5821 ctgttgtttg gatcaacctt tccgagtcca agaaattggt cctgcgtaag acctagttgc
     5881 tgaagatatg tctcgccaaa acaacgcatc accaatcctg
//
LOCUS       DASSFP010000045         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1200881, whole genome shotgun sequence.
ACCESSION   DASSFP010000045 DASSFP010000000
VERSION     DASSFP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200881"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2884
                     /locus_tag="VFH91_00985"
     CDS             <1..2884
                     /locus_tag="VFH91_00985"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861579.1"
                     /translation="MGESPIRGNVSHGDGMYKSNDGGKTWKKIGLDDTRQISRIRVHP
                     RNPDLLYVAALGHVWGTNDQRGVFRSKDGGKTWEKVLTRGNKAGAIDLSMDPTNPSVL
                     YAGFWEVYRKPWTLESGGPGSGLFKSTDGGDTWTELTRNPGLPRGVVGKVGITVSPAN
                     PDRVWAIVEAEDGGVFRSDNAGRTWTKVNEERRLRQRAWYYSRIYADPRSADTVYVLN
                     TGFYRSNDGGRTYTGIQVPHGDNHDLWIAADDPNRMIESNDGGSNVTYNGGRSWTEQD
                     QPTAQFYRVALDNDFPYNIYGAQQDNSTVRIASRTSEGSIGTSDWYDVGGGESGWVAP
                     SPKDSQIVFAGSYGGLTTRYDRRTGQLRDINPYPNNPMGSGADALKYRFQWNFPLLFS
                     PNDPNTLYCAANVLFKSRDEGSSWEIISPDLTRNDKTKQASSGGPITKDNTSIEYYGT
                     IFTLMESTLQPGTIWTGSDDGMIYLTRDGGKNWSNVTPPTSLMPEWIQINSIEASPID
                     PGTAYVAATMYKWDDNRPYLYRTTDYGKTWKKITNGIPETTFTRVIREDPNKRGLLYA
                     GTENGMYVSFDDGEHWQSFQLNLPIVPITDLAIQKRDKELVVATQGRSFWILDDLPLL
                     HQLMDSGGLTSVNETQLYKPRESYRMPGGGSPLGPTATAGRNPAPGVVVYYSLKAKPA
                     ENIELEFLDSSGKSIRKFTGRVGKAAAAGGAAPAPAPPPDEGGFFGGGATPSLPVEIG
                     LNRFVWDTRYPDAARFAGMILWAGETRGPKVSPGNYQVKLTVDGKALTQGFEIKADPR
                     LSTTQPDYAKQLELGLKIRDKLSETNNAIVQIRDVRKQVEDLLKRLTGQPNFKTVNDA
                     GTALNKNLTEVEEALYQTKNQSSQDPLNFPIRLNNKLAALGGVVGGAEAAPTAQSYAV
                     YDEVVGQIDSQLQKLAQLMKKDVPAFNQLVRDQNIPAVVVKPEGNR"
     gene            2947..>4044
                     /locus_tag="VFH91_00990"
     CDS             2947..>4044
                     /locus_tag="VFH91_00990"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET6861580.1"
                     /translation="MNRENVTAGAIARLTEANLTGDPSIVITDVSHDSRRAASGTLFA
                     AVSGAVFDAHRFVPQVMSQGAAGVISEQFAPESFPGVWLQVGNLRRAMALAAAEVQHH
                     PSRELQLVGITGTNGKTTTAYLIASIPEQAGEPVAMTGTVEYRLGKERRKADRTTPEA
                     PDMQRLLREAVGIGCRTAVMECSSQAMDFHRCDELEYAVAVFTNLTRDHLDYHKTMEN
                     YWYAKLRLFDGRLGTRPRVSVINVDDPKGVELAESLARDGLKVVRYALNADADVKAIN
                     PEFSLKGMEFQLTTPAGNRDFHSPLVGPPHVYNTLAAVASGLALGYSLDVITAALEKC
                     TGAPGRFERVPHSGDFAVVVDYAHSDDALLNV"
BASE COUNT         1037 a          977 c         1117 g          913 t
ORIGIN      
        1 gatgggtgag tctccaatac ggggaaatgt ttcgcacggc gacggcatgt ataagtcaaa
       61 tgatggaggt aaaacctgga agaaaattgg tctcgatgat acgcgccaga tatcgagaat
      121 cagagttcat ccaaggaatc cggatcttct ttacgtggca gcactcggac acgtctgggg
      181 aacaaacgat caacgtggag tcttccgatc gaaggatggc ggcaaaacct gggagaaggt
      241 tctaactcgc gggaacaagg cgggcgcgat cgacttaagt atggatccaa ccaatccttc
      301 agtgttgtac gccggattct gggaggtgta tcgcaaacca tggaccctgg agagcggtgg
      361 cccgggcagc ggacttttca aatccacaga cggtggagac acgtggacgg agctcaccag
      421 aaatccagga ttgccgcgag gcgtcgtcgg aaaggttgga attacggtct caccggcaaa
      481 tccggacagg gtttgggcca tcgttgaagc tgaagatggt ggggtgttta gatccgacaa
      541 cgctggtaga acatggacca aggttaacga agaacgccgg ttacgccagc gcgcctggta
      601 ctactcgcgg atttacgccg atcccaggag tgcagatacc gtttacgtgc tcaacactgg
      661 attttatcgc tctaacgacg gcggtcgaac ttacacaggc attcaggttc cgcacggaga
      721 caatcatgac ctttggatcg ctgcagacga tcccaatcgg atgatcgaaa gtaacgatgg
      781 aggatcaaac gtcacctata acggcggacg aagttggacc gagcaggacc aacctaccgc
      841 tcagttttac cgggtcgctt tggataatga ttttccctac aacatttacg gtgcccaaca
      901 agataactct accgtgcgaa ttgcgagtcg tacgagtgaa ggaagtattg ggaccagcga
      961 ctggtacgac gttggcggtg gcgagtcagg ttgggtagca ccgtcgccga aggattctca
     1021 gatcgtcttc gccggttctt atggaggact cacaactcga tatgatcgtc gcactggaca
     1081 gttgagggat atcaacccgt acccaaacaa cccgatgggc tcgggcgcag acgctttaaa
     1141 gtatcggttt caatggaatt ttccacttct gttctcgcca aacgatccca acactcttta
     1201 ctgcgcggcg aacgtactct tcaaatcaag ggacgaggga tccagctggg aaataataag
     1261 ccccgatcta actcgaaatg ataaaacgaa gcaggcatct tctggtggcc caatcacaaa
     1321 agacaacacc agtattgagt actacggcac gattttcaca ctcatggagt cgacgttaca
     1381 gcccggaact atctggacgg gaagtgatga cggaatgatc tatctgaccc gggacggtgg
     1441 aaagaattgg tccaatgtaa cgcctccaac gagtctcatg cctgaatgga tccagattaa
     1501 ctcaattgaa gcttcgccaa tcgatcccgg caccgcatac gttgcagcga ctatgtacaa
     1561 gtgggatgac aaccgaccct atctctatcg gacaacagat tatggaaaga cgtggaaaaa
     1621 gatcaccaac ggtattcctg aaactacttt cacccgtgtc attcgcgaag atccaaacaa
     1681 acgcggtctt ctttatgctg gaacggaaaa cggaatgtat gtctcatttg acgatggcga
     1741 acattggcaa tccttccagt tgaatctgcc gatcgtgcca atcacggacc ttgcgattca
     1801 aaaaagagac aaagagctcg tggtcgcgac ccagggaagg tcgttttgga tactggacga
     1861 tctgccgctc ttacatcagt taatggattc aggtgggctg acctctgtga acgaaacgca
     1921 gctatataaa cccagagaga gttaccggat gccgggtggt ggttctccgc tcggtccaac
     1981 tgcaacggca ggtcgaaatc cggcgccggg tgtggttgtt tactattccc ttaaggcaaa
     2041 acctgccgag aatatcgaac ttgaattcct cgatagttcg ggtaagtcca tccgcaaatt
     2101 cacgggacgt gttggaaaag cagctgcggc aggtggcgct gcaccagctc ccgcccctcc
     2161 gcccgatgaa ggtggatttt tcggcggtgg tgctacacca tcattgccgg tggaaattgg
     2221 cctcaatagg tttgtgtggg acactcggta tccagatgct gcgaggtttg ctggaatgat
     2281 tctttgggct ggcgaaactc gtggtccaaa ggtttcgccc gggaattatc aggtgaagtt
     2341 aacagtcgat ggcaaggcct tgactcaagg ttttgaaata aaggcagatc ctcgtttgag
     2401 cacgacccaa ccagattatg caaagcaatt ggagctcgga ttgaaaatcc gagacaaact
     2461 cagcgagact aacaatgcaa ttgttcagat tcgtgacgtg cgaaaacaag ttgaggatct
     2521 gttaaagagg ttgaccgggc aaccaaactt taagacggta aatgacgccg gcactgcgct
     2581 caacaaaaac ttgactgagg tcgaggaagc gctgtaccag actaaaaatc agagcagtca
     2641 ggatccactt aactttccga tccgtctgaa caacaaactg gcagcacttg gcggtgtagt
     2701 gggtggagca gaagcagcac ctacggctca gtcttatgct gtttatgacg aagttgttgg
     2761 ccaaattgat agccagttac agaagttggc ccagctaatg aaaaaagacg tacctgcgtt
     2821 caatcagctg gttcgagatc aaaatattcc ggctgtcgtt gtgaagccgg aaggcaatcg
     2881 ataagcttga acagtaggaa gtgggggtga tcgtcatccc cactaccgaa gcaattggga
     2941 gtagttgtga acagggaaaa tgttacagca ggggcaatcg ctcgcctcac tgaggccaat
     3001 ttaacgggcg acccatcaat cgtaattact gacgtcagtc acgactctcg tcgtgcggcc
     3061 tccgggactc tttttgctgc ggtgtccggc gccgtctttg atgcgcacag atttgtgccg
     3121 caggtcatga gtcagggagc cgcaggtgtc atctctgaac agtttgcgcc tgagagtttt
     3181 cctggtgtgt ggttgcaagt gggtaatctc agacgggcta tggcgcttgc ggccgccgaa
     3241 gtgcaacacc atccgtcgcg cgagcttcag ttggttggta tcacgggaac caatggcaag
     3301 accactacgg cgtatctcat tgcctcgatc cccgaacaag ccggtgaacc ggtggcgatg
     3361 actggcacgg ttgaatatcg tctcggtaaa gaacgacgca aagctgatcg gactactccc
     3421 gaagcacccg acatgcagcg tctgttgcgt gaggcggttg ggataggttg ccgaacagca
     3481 gtcatggaat gttcgtccca ggcaatggat tttcatcgtt gcgatgagtt ggaatacgca
     3541 gtggcagtat ttacaaacct cacgcgcgat cacctcgact atcacaagac gatggagaac
     3601 tactggtacg caaagctccg cctattcgat ggcaggttag gtactcgacc acgagtctcg
     3661 gtaattaacg tcgatgaccc aaagggggtc gagttggctg aatcgttggc tcgcgatggc
     3721 ttgaaagttg tccgttatgc acttaacgct gatgctgatg ttaaggctat caacccggaa
     3781 ttctcattaa aaggtatgga gtttcagctt accactcccg caggcaaccg cgactttcat
     3841 tcgcctctag ttggcccacc gcatgtttat aacacgctcg cggcggttgc gagtggtctg
     3901 gcccttggat acagcctcga tgtgattact gcagctctcg agaagtgtac cggcgcgcca
     3961 ggtagattcg agcgagttcc acacagcggt gattttgcgg tggtggttga ctatgcccat
     4021 agtgacgatg cgcttttaaa tgta
//
LOCUS       DASSFP010000046         5216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1200936, whole genome shotgun sequence.
ACCESSION   DASSFP010000046 DASSFP010000000
VERSION     DASSFP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200936"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFH91_00995"
     CDS             complement(<1..438)
                     /locus_tag="VFH91_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861581.1"
                     /translation="MYSAQITRSNPTCIVLLLDQSGSMSDPFSGDTASKKSDFVAEVV
                     NHTLHDLVIRCTKTEEVRNYYYVAVVGYGRVVGPAFRGPLASRNLVSIADVADYPLQV
                     KSSYKRVADGAGGWVEIPVRYPVWINPLADGKTPMCEALNLVKN"
     gene            complement(444..1316)
                     /locus_tag="VFH91_01000"
     CDS             complement(444..1316)
                     /locus_tag="VFH91_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861582.1"
                     /translation="MENKTCSYCGFIAQQSAEFCVSCGRELPASVTEERKSFEAPANQ
                     LYSIQPFTGIGAVINPTLRIFTKNFWFISKLVFVIFAPLEMIKVLGVGEANASWQNVI
                     GAGFLWVLCNALIAPSLIYALVTQMQTGVTPSLHESYRWGLRKLLPFIACVTLAWFLT
                     ALGFALLIIPGVILALAFELVYPMATLENGSPVEILKRSYNLTKGYRWNIFAATLVLG
                     MLCAAVSFPINAVSTILTVSGSAFWPVQVALAIIVDIIGEATTVLALVIYLSILSTAV
                     RRETSENFSVTYER"
     gene            1827..2699
                     /locus_tag="VFH91_01005"
     CDS             1827..2699
                     /locus_tag="VFH91_01005"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HET6861583.1"
                     /translation="MNTKTLLLDHVDMRPVTANDQEASDRTLVQLDPDHPGFRDPEYR
                     ARRNSIAAIALSYRKGEPIPPAPYTSIENEVWSTIWNALGPAHEKYACAEYRECLKRL
                     AFPSDRIPQLNEVSEKVEALSGFRLEPVAGLVEPRIFLESLADGIFLCTQYIRHHSTP
                     LYTPEPDVAHEIIGHAVTLASDRLAQLNRLFGEAVRRTRNESDLDRLSRVYWFTIEFG
                     VLLEDGDLKAYGTGLLSSAGELEAMHKAELRPLNLEAAASHEYDPTHFQSVLFCAESF
                     ASMDQTLREFLAKW"
     gene            3003..>5216
                     /locus_tag="VFH91_01010"
     CDS             3003..>5216
                     /locus_tag="VFH91_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6861584.1"
                     /translation="MISRKIGLSLPLLILAVGLFLNSTVLAQSTATVQGTVTDSKGAV
                     LPNVTITIRNRSTSFERTIQSDSQGSYQIAALPVGTYSIEARVDGFKTQVVEQVSVEV
                     AKTQVQNFQMEVGALTEQVLVTSDLPIVETATTSVGTVINQRTVQEIPLNGRHFVDLG
                     LLIPGSVTPPQNGFLTAPLRGQGSFGLNTAGNREDTVNFMINGVNLNDMAQNQITFQP
                     SINTVQEFKADNSTFSAEYGRNSGAIINIATRSGTNDYHGEFFEFLRNDALDARNFFE
                     RTPQPAPFKRNQFGFNIGGPVNFPHFGEGGPVFGYEGKNKTFFFFSYEGLRQRQGLTI
                     SGVTVPTDAQRAAVTDPVILKLLPLIPRPNVGTNGFAGSATAPVDIDQWTLDISHTIG
                     VNDRVHGYYAIQRDQRGEPILQLNTLPGFGDTRKSRRQIFTLNETHTFNSAVVNEARF
                     GFNRIHILFSPNAKLNPLDFGIQDGVKEPIGLPQMNVTGANLNFGGPANFPQGRSDTT
                     FVVSDTLNYLRGNHSLKFGLEWRRFLNNNTNKDTGSFNFANLNSFLQGTANTFTVTLG
                     DVSTAIAQGALGLFAQDNYKLRPNLTLELGFRYDWLMSPTERYDRFVDYVPETNSLVR
                     VNNGIAPVYKTNSKNFQPRLGFAWDPFKDGKTSVRGAYAILADQPVGNLVSPNGSNPP
                     FATAVALPANTTTQLSNATSVAIPGATVSPTSSDPEFDNAYVQSWNLNVQREVANG"
BASE COUNT         1341 a         1316 c         1282 g         1277 t
ORIGIN      
        1 attcttaacc aggttgagcg cctcacacat aggcgtcttt ccatctgcta acgggttgat
       61 ccaaaccggg tagcggaccg ggatttcgac ccatcctccg gcgccgtccg caaccctttt
      121 atagctgctc ttgacctgga gtgggtaatc agccacatcg gcaattgaga cgagatttcg
      181 cgaggccagg gggccacgaa aagcgggacc aaccacacgt ccatacccga caacggcgac
      241 gtaatagtag tttctgactt cttcggtttt tgtacatctg attaccaggt cgtgcagcgt
      301 gtggttaacc acttccgcta caaaatcgga cttctttgac gccgtatcgc cgctgaaagg
      361 atctgacatg gagccggatt gatccagaag caggacaatg caagttggat tactgcgtgt
      421 tatttgagct gaatacattt gacctagcgc tcgtaagtga cggaaaagtt ttcagacgtt
      481 tcgcgacgaa ccgctgtcga aagaatgctt agatagataa ctaacgcaag taccgtggtt
      541 gcctcgccga taatgtctac aataatcgcc agcgcaactt gtaccggcca aaatgctgat
      601 cccgaaacgg ttagaatcgt cgaaacagcg tttatcggaa aactcaccgc agcacacaac
      661 attcctaaga caagagttgc ggcaaaaatg ttccatctgt aacccttggt caggttatag
      721 ctgcgtttca agatctcgac cggactgcca ttttccagtg tggccatcgg ataaaccagc
      781 tcgaaagcga gagccaggat gacgcctgga atgatcagca aagcaaatcc caatgccgtt
      841 aaaaaccagg caagggttac acaggctatg aatgggagaa gttttctcag cccccagcgg
      901 taagattcgt gcaggctggg agtgacacca gtctgcatct gggtgaccag agcataaatc
      961 aacgatggcg caatcaaggc gttacacagc acccagagaa aaccagctcc aattacgttc
     1021 tgccagctcg cattcgcttc cccaacgcct aaaactttaa tcatctctaa tggcgcaaaa
     1081 atcacgaaaa caagcttcga aataaaccag aaatttttag tgaatattct cagcgtggga
     1141 ttgatgaccg cgccaattcc ggtgaacggt tgaattgaat atagctgatt tgctggtgcc
     1201 tcgaacgatt tacgttcctc ggtgacggat gcagggagtt ctcggccaca actgacgcaa
     1261 aactcagctg actgctgagc gataaagccg cagtatgaac aggttttatt ttccattgag
     1321 accttccaga cagttagtaa tcagcctttt cgatctgccg atattctgaa attgtgagag
     1381 agccgagccg gaatctgagt gatgccgcaa gggatcaaga gtcaagatag cgtaagagga
     1441 tcggagaggg caacggaaga aagcggatca ttttccatat atcatttttc atttttcagc
     1501 gtcgtgctac cgcgcctgaa aaatggaaaa tgaaaaatga tatatgaaaa atgatcagga
     1561 tcttgacgga ctcagaggtt ggtgcgtatt ctgtcgacgt agagacgcca tgaactgcag
     1621 ccagtatcgc tattattact attacggcca gctctccact ggagggacgg tcgggcgatg
     1681 ggctgtcggt actaacagcg tttagtatct aagacaagat tcctgaaacc ggccaccaat
     1741 ctccagcaga tggtggccgg tttttttgtg ctttctcaat gtaaacggaa accaatgcgg
     1801 aaatgcaccg cttcgaggcg aagaccatga acacgaaaac actcttgctc gaccacgttg
     1861 atatgagacc cgttacggca aacgatcagg aagcgtcgga ccgaactctg gtccagctcg
     1921 atcccgacca tccaggattt agagatcccg agtatcgcgc tcggcggaac tctatcgccg
     1981 ctatcgcctt aagttatcga aagggtgagc ccatcccacc tgctccgtac acatcgatcg
     2041 agaatgaagt gtggtcaacc atttggaacg cgcttggacc tgcacatgaa aagtatgcgt
     2101 gcgccgaata ccgtgaatgt cttaagcgcc tggcatttcc ctcagaccgg atacctcaac
     2161 ttaacgaggt tagtgagaag gtcgaagcct taagcggttt tcgccttgaa ccagtggccg
     2221 gcttagtcga gccacgaatc ttccttgagt cgctggctga tggaattttt ctctgcactc
     2281 aatacattcg tcatcactct accccgctgt acacgcctga gcccgacgtt gctcacgaaa
     2341 tcattggcca cgctgtgacg ctcgcaagtg accggttggc acagctaaat cgattatttg
     2401 gcgaagcggt acgccggaca agaaatgaaa gcgatctaga tcggttgtcg agagtgtact
     2461 ggttcaccat agagttcgga gtcttgcttg aagatggcga tctgaaggct tatggcacgg
     2521 gcctgttgtc gtctgcgggc gaattggaag caatgcataa ggctgaactc aggcctttga
     2581 atcttgaggc agccgcgagt catgagtacg acccaactca ttttcagtcg gtcctattct
     2641 gcgcagaatc gtttgcttca atggatcaga ccttaagaga gttcttagcg aagtggtagc
     2701 aactttgagc ttcaaaattg tcggcgatga agcgcgatct ctgtgctttc ggatgagctt
     2761 gtagttttcg gatacgtctg tctttacaac aaagaaaagt agttgatttt gttaggaaat
     2821 ttagcatggt ccagcggcat tgatgttgct tagagcattt ttaactcccc ctctttcaaa
     2881 aggataactt tcggcgattg cctcgggctc gtttgctggc atgaagccac aaacgaatcc
     2941 cggcgcctca caggcttacc gggctggacc gaggtgtatt tgcagctgtc aggaggtcat
     3001 tcatgattag cagaaagatt ggtctgtccc tgccgttact catacttgct gttggccttt
     3061 ttctgaactc caccgttctt gctcaatcga ctgctaccgt ccaggggacg gttaccgact
     3121 cgaaaggcgc cgtgctgcca aacgtgacca tcactatcag gaatcgcagt acgtcgtttg
     3181 aacgaacaat ccagtctgac tctcaaggta gctatcaaat cgctgcctta ccggtcggca
     3241 catactcaat cgaagcacga gttgacggct ttaaaactca agtcgtggag caggtctcag
     3301 tagaagttgc caaaacgcag gtgcaaaact tccagatgga ggttggggcg ctcacagaac
     3361 aggtactggt cacctcggat ctgccaatag tcgaaaccgc tacaacttcg gtaggaaccg
     3421 ttatcaatca acggacggtt caggaaattc cattgaatgg tcgtcatttt gtcgatctgg
     3481 gtctactaat accggggtca gtaactcctc cacaaaacgg ttttctaact gcgccgcttc
     3541 gtgggcaggg ttcgtttggt ttgaacacgg ctggcaatcg tgaagacaca gtgaatttca
     3601 tgattaacgg tgtaaatcta aacgatatgg ctcaaaacca gattacgttc cagccgtcca
     3661 tcaataccgt gcaggagttt aaagccgata attcaacgtt cagtgctgag tacggcagaa
     3721 actcgggagc aattataaat attgccaccc ggtccggaac gaatgattac cacggggaat
     3781 tttttgaatt tcttcgcaac gatgcgttgg atgcccggaa tttttttgaa aggacgccac
     3841 agccggcgcc attcaaacgc aaccagttcg gattcaacat cggtggaccc gtgaatttcc
     3901 ctcacttcgg tgaaggcgga cctgtttttg gctacgaagg taaaaataag accttcttct
     3961 tttttagtta tgaaggcctc cgtcagcgac agggactgac gatttctggc gtgaccgttc
     4021 caactgacgc gcaacgtgcc gcagtcacag atccagtcat actaaaactc ttgcctctta
     4081 ttccgcgccc aaatgtgggc accaacggtt tcgctggttc tgcaaccgca ccagtcgaca
     4141 tcgatcaatg gactctcgat atcagtcata ctatcggagt caacgatcgt gtccacggtt
     4201 actatgccat tcagcgcgat caacgaggcg aacctatact tcagctcaat actcttcctg
     4261 gctttggcga cactcgcaag tcgcgtcgtc agatcttcac tttgaatgaa acgcacacct
     4321 tcaactcagc agtcgtgaac gaggcccgct ttggtttcaa ccgcattcac atactgttta
     4381 gtccaaatgc aaaattgaac ccgctcgatt tcgggataca ggatggtgtg aaagagccga
     4441 ttggactgcc gcagatgaac gtaactggag cgaacctgaa cttcggcgga cctgcaaact
     4501 ttccacaggg acgttccgat acaacgtttg tggtctccga cacattgaac tatctgcgcg
     4561 gcaatcactc gctaaagttt ggcctggagt ggcgccgttt tctaaacaac aacaccaaca
     4621 aagacacggg ctcgttcaac ttcgccaacc tcaactcgtt ccttcaagga accgctaaca
     4681 cttttaccgt gactttggga gacgtctcga ctgcgatagc acagggcgcc ctgggattgt
     4741 tcgctcaaga caactacaag ttgcggccaa atttaacttt agagttagga tttcgttacg
     4801 actggttgat gtcgccgact gaacggtatg accgtttcgt ggattatgtt cccgaaacaa
     4861 actcgcttgt tcgtgttaac aacggaatag ctccggtcta taagacgaat tcgaaaaact
     4921 tccaaccgcg tctcggattc gcgtgggatc cgttcaagga tggtaagacc tcggttcgtg
     4981 gtgcgtatgc cattttggcc gatcagcctg tgggtaacct ggtcagcccc aacggctcga
     5041 atccgccttt cgcaaccgcg gtagccttgc ccgccaatac aactacgcag ctttctaacg
     5101 cgacttcagt tgccattcct ggtgctacag tctcgccaac ttcatcggat ccagagtttg
     5161 acaatgccta tgtccagtcc tggaacttga atgtgcagag agaagttgcg aacggc
//
LOCUS       DASSFP010000047         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_755040, whole genome shotgun sequence.
ACCESSION   DASSFP010000047 DASSFP010000000
VERSION     DASSFP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755040"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFH91_01015"
     CDS             complement(<1..338)
                     /locus_tag="VFH91_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861585.1"
                     /translation="MGLFWRRKSGDQFVTLGLNEPKTAVKDESASDLAGEVPQPRIPE
                     PVLPVPEIIPPVTASSPDPLPLQPETIEKPPIINRPAVTVDKPVPSRSPFATSVLGLN
                     LSMEELQAQEA"
     gene            complement(702..2534)
                     /locus_tag="VFH91_01020"
     CDS             complement(702..2534)
                     /locus_tag="VFH91_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6861586.1"
                     /translation="MPEKIGRIRLLYIVLGVLLLVGLLPLTFAGFVLSGRSADELRSI
                     EGRYQAQLVQDKARQIELYGQRYRDVVTGLARAFEIAGGIQAFDEKGYDLRLQKTLQE
                     DPNLIALALWPVDGKLHRAFQPDVIKLDEVDARVSEVLAKMSGRGLVVSRPQIIRSGQ
                     EMALTIAEPVLGGPDNQSVVAAVVAVVSFQEVFSAVQQPTAQSERELLDAGLPVVFVV
                     DQNGRAVAHPEASVAFAEKSMTDLKVVQDWQESGSQVQSALAPFSAVRNGRTVEMLGS
                     YATAELDKNSRLGVIAIQDEAAALASVADMRWQTLWISLVAALLTIIIGLFFANKLTD
                     PVRELALGAHRIASGDFSQRIQVKSRTELGDLGNSFNLMTDQIEHYIGDLQQSAQANR
                     ELFIGTVKGLAAAIDGKDPYTRGHSERVSRFSIAIAQRMGLPDDEIEKIRISALLHDV
                     GKIGIDDNILKKPAALTDDEYEVMKKHPQKGYKIMSQIPAMKEFLPGMYMHHEMVDGK
                     GYPQGLKGEEIPIMGKIVAVADTFDAMTTDRPYQKAMKFEDAVARIESFVNTRYDPDV
                     VAAFTAACREGQIRPGSVRLKKPTSAVPQHDLMSAPDDARVPVS"
BASE COUNT          695 a          763 c          716 g          697 t
ORIGIN      
        1 gcttcctgcg cctgcaactc ttccatcgac aagttgagtc ccagcactga ggtcgcaaat
       61 ggagagcgac ttggtactgg tttatcaacc gttaccgcag gtcgattgat aattgggggc
      121 ttctcgatcg tttcaggttg aagcggcagg gggtccggcg atgaagcagt taccggagga
      181 atgatttccg gtactggtaa cacgggttca ggtattcgcg gttgcggaac ttcaccggct
      241 agatcgctgg ctgactcatc ctttacagca gtcttaggtt cgttcagacc aagtgttaca
      301 aactgatccc cgctctttcg tctccagaaa agtcccataa ttgcttttgc gttcgctgtg
      361 ctccgtacaa tttatttgtt tggattataa gcacaagtac accggacccc caaaagcatg
      421 agtttttagg cactgaaaga taagtgagat atgtcaatga ctcactttag aaggtagtaa
      481 cctaatttga gctctcctct gtgcaacctc tgtgtccttt gtgactcggt ggtggttttt
      541 gctgcagaac agttaaccac aaagacacaa agtacacaga ggttgcacag agaaaccaaa
      601 ttaagaccaa cttttagaaa gtttggagtt ttggggagga caactgaagg taacagaccc
      661 aaatcctgaa gttggaaaga ggtaacctca gatctacgca cctagctaac cgggacgcga
      721 gcgtcgtcag gcgccgacat taaatcgtgt tgaggaaccg cgcttgttgg ctttttgagt
      781 cgcacggagc cgggtctgat ttgcccctcg cggcatgcag cggtgaaggc agccacaaca
      841 tcggggtcat atcttgtgtt cacaaaactc tcgatgcgcg ccactgcatc ttcaaacttc
      901 atagcctttt gataaggccg atcggtcgtc atcgcgtcaa aggtgtcagc gactgcaacg
      961 atcttaccca tgatcggaat ttcctcgccc ttgagtcctt gcggatatcc cttgccgtcc
     1021 accatctcat ggtgcatgta cattcccggg agaaattctt tcatcgccgg aatctgagac
     1081 atgatcttgt atcccttttg gggatgcttt ttcataactt catattcgtc gtcagtaagt
     1141 gccgcgggtt tcttcagaat gttatcgtcg atgccgattt tgccgacgtc atggagcaaa
     1201 gcgcttatcc tgatcttctc aatttcatca tccggcaatc ccatacgctg ggcgatggcg
     1261 atcgagaaac gagaaacacg ttccgaatga ccgcgagtgt aagggtcttt accgtcaatc
     1321 gcggccgcca atcccttcac agtgccgatg aacagctcac gattcgcctg tgctgactgc
     1381 tgcagatcgc cgatgtagtg ctcgatctga tcagtcatca ggttgaacga atttccgaga
     1441 tcacccagtt cagtgcgact cttaacctga attcgctgcg aaaagtctcc agaggcaatg
     1501 cgatgagctc cgagtgcaag ttcacgaacg ggatccgtca gcttattggc gaagaagagt
     1561 ccgatgatta tggtcagaag cgcagccact aaacttatcc agagcgtctg ccaacgcatg
     1621 tcagctacgg aagccaacgc ggctgcctcg tcctgaatcg cgatcactcc aagccgcgaa
     1681 ttcttgtcga gctcggccgt cgcatacgaa cccagcatct cgactgttcg gccatttcga
     1741 acagcagaaa atggagccag tgccgattga acctgggaac ccgattcctg ccagtcctgg
     1801 accaccttca ggtccgtcat cgatttttca gcgaatgcca cactcgcctc gggatgggcc
     1861 actgcacgtc cattctggtc cactacaaag acaaccggaa gacctgcgtc gagtagttcc
     1921 ctttcacttt gagccgtagg ttgctgcacg gcactgaaaa cctcctggaa tgagacgacc
     1981 gcaaccacag ctgccaccac gctttgattg tcaggtccac ccagtacagg ttcggcgata
     2041 gtgagcgcca tctcttgtcc ggagcgaata atctgaggcc gacttacaac cagtccacgc
     2101 ccactcattt ttgcaagaac ttcactaacg cgagcatcaa cttcgtcaag cttgatcacg
     2161 tctggttgaa aggctcgatg aagttttcca tcgacgggcc acagagccag cgcgataagg
     2221 tttggatctt cctgaagtgt cttctgcaaa cgaaggtcgt aacctttttc atcgaatgcc
     2281 tggattccgc cggctatttc gaaagcccgc gccaacccag tcacgacgtc gcgatacctt
     2341 tggccgtaga gttcgatttg acgggctttg tcctgtacta attgagcctg gtaacgccct
     2401 tcgatagatc gcaattcgtc agcgcttcgt cccgaaagaa cgaaaccggc aaaggtaagc
     2461 ggcaggagtc cgaccaaaag caaaactccg agcacaatgt agaggagacg tattcgtccg
     2521 attttttccg gcatttttaa gtgtcgaacg ctacaattcc ggcgggcgtg ggcttgcacg
     2581 actaagaaaa cgcggtagct gggcgccaca gcgacacggg ctattattgt tgactaaccg
     2641 ctttgctgtc aacgagattt accatcgttt aacacaagtt ggtggacgga ttaggtaaaa
     2701 tttatcgacc taacgtcccg ccccaacgtc taaatcgttg agcggtatga cacaacggcc
     2761 actcaccagt cgcatcgcca gctgagttcg cggtcgaatg gacactctaa ccaacgacga
     2821 aacgattgtg gaacgagcac tggccggaga tccagaggct tttggggaga t
//
LOCUS       DASSFP010000048         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_755749, whole genome shotgun sequence.
ACCESSION   DASSFP010000048 DASSFP010000000
VERSION     DASSFP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755749"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFH91_01025"
     CDS             complement(<1..536)
                     /locus_tag="VFH91_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HET6861587.1"
                     /translation="MNRTTARQPPVRYKQPFKTRIVFALCFVLLTLCVACQQQQAKQQ
                     APATPDTREADVAALKEWDANWSKAAGAKDVDKTVSYYADDVIVQPPNGKIATAKADI
                     RKIWADMLSAPGFGGGWQPTKIEVARSGELAYLSGTYEFTFKDASGKPTTDRGKFVEI
                     VKKQPDGSWKCVNDIWNS"
     gene            complement(611..1687)
                     /locus_tag="VFH91_01030"
     CDS             complement(611..1687)
                     /locus_tag="VFH91_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6861588.1"
                     /translation="MKYRVLGNTGLKVSELCLGTMTFGENFFQIAVVDQSGVNKMVSR
                     SFDAGINFFDTADVYSYGQSEEVLGKALKELPVGREKVIVATKVRSAMSQEATEGTGD
                     INNVGLSRQHIFASVEKSLQRLGTDYIDLYQVHGWDVLTPVEESLRALDDLVRQGKVR
                     YIGCSNWAARQLMKALCLARENNMSQFVSLQAYYSLAGRDLEHELLPLCLEENVGVLP
                     WSPLSGGFLSGKYRRENKNPEGARRTQFEFPPVDEERGYDAIELMDQIAKEKGATVAQ
                     VALAWLLHQRGVTSVIIGANKMTQLEDNLKAAELNLTTDEFERLSATTQPRQLYPEWM
                     IERQNAGRTETTPASTSARTATSK"
BASE COUNT          635 a          707 c          656 g          734 t
ORIGIN      
        1 tccgaattcc aaatatcgtt gacgcacttc caggagccgt cgggttgttt cttaactatc
       61 tctacgaact ttcctcgatc agtcgttggc ttgccactcg cgtccttaaa cgtaaattca
      121 tacgtaccgc tcagataggc caattctccg gatcgcgcca cttctatctt tgtcggctgc
      181 catcctccac caaaaccggg agctgacaac atgtctgccc agatcttgcg aatgtcggcc
      241 tttgcagtgg cgattttacc gtttggtggt tgcacaatca catcgtcggc atagtacgaa
      301 acagtcttat ctacgtcttt agctccggca gccttggacc aattagcgtc ccactctttg
      361 agagccgcta cgtcggcttc tctggtgtcg ggagttgcgg gtgcttgctg ttttgcttgc
      421 tgttgttggc aagcgacgca aagagtcaga agtacaaagc aaagcgcaaa tacgattcgg
      481 gttttgaaag gttgtttgta acgaacggga ggttgtcgag cagtggttct attcatgatg
      541 agtctccatt actggggttc ggagagcgcg accaccctaa ttccgaggcg ttaacttgtc
      601 aatgttgctg tcactttgac gtcgcggtgc gtgctgaggt tgaagccgga gtcgtttcag
      661 tgcgtcctgc attctgacgt tcgatcatcc actcgggata gagctggcga ggctgtgtag
      721 tcgcggacag tcgttcaaac tcgtctgttg tcaaattcaa ctcggcagcc tttaagttat
      781 cttcgagttg agtcattttg tttgcgccaa taatcacaga agtgacaccc cgctgatgaa
      841 gtagccacgc taatgcaact tgagccactg tcgcgccttt ttctttagcg atctggtcca
      901 tcagttcgat cgcatcgtaa ccacgttctt catcaaccgg tggaaattca aactgcgtcc
      961 gacgagcgcc ttcaggattc ttattttctc gccggtactt acctgataga aacccgcctg
     1021 aaagcggcga ccacggtaaa actccaacgt tctcttccag acagagcggc aataactcgt
     1081 gttcgagatc acgaccagcc aatgaatagt atgcctggag tgaaacaaac tggctcatat
     1141 tgttttcgcg cgccagacaa agcgctttca tcaattgacg cgcagcccag tttgaacagc
     1201 cgatgtaacg aacttttccc tgacggacca gatcatcaag tgcgcggagt gattcttcaa
     1261 ctggcgtgag aacatcccaa ccatgaactt gatatagatc gatgtaatcc gttccaagtc
     1321 gctgcaggct tttttcaaca gaggcaaaga tgtgctgtct tgagagaccg acattattta
     1381 tatcgccggt accttccgtc gcttcctgac tcattgcgct ccggactttg gtagcaacga
     1441 ttactttctc acgaccgaca ggtaattcct tcaatgcttt acccagaacc tcttcagatt
     1501 gcccgtagct gtagacgtcc gccgtatcga agaagttgat tcccgcatcg aatgaccgcg
     1561 aaaccatctt gttgacgcct gattgatcga cgactgctat ttgaaaaaaa ttttcaccaa
     1621 acgtcatcgt tcctaaacac aattccgaaa ccttaagacc tgtgttgcct aacactcgat
     1681 atttcatata gcgactcctt ttatctttct ctcgctgatt ccataggtat agtaggttga
     1741 gtttacctaa aacccaacgc caaaagtttc caagtttggt tcaggattat agtgatttgc
     1801 tattactctt tatcccatgt gcatttggca ttctacagct cttgaggggt caagcgaacc
     1861 ggcggtcgga tactggtgca gtcgaatggt agtgcagttt gggatgtccg atatgcttta
     1921 gcttgtcggt cgtcgttgac tcgctcctct gaacttgagt gtgcatcatg accgacaagc
     1981 taataaagca tatcggacat cccaattgga gaattgccga caaacttgtg gcgccacctc
     2041 gcaatagcgg cggccagttt tgaatgatcg accaaatgct cgaaaaaatt aacgagtgat
     2101 gggtgatcca attcgcccca atttgacgcg ttaataggtg ggaaggttga taggcgaatg
     2161 tcgaataagg atgaatttac aagtcgcaat gattgccggt ctatggatcg tcttggtggt
     2221 taacccggca tccaaggtgg acgatgatta tcaggattaa atagttctaa agtttcgagc
     2281 acacactgtt tgaaggggcg gtcccactct ggtccgcccc ttctttttgg gcccggacca
     2341 cagtttcacg acaccacagg cttcagccgc gtggtccatc aacttcaacc tagccccgcc
     2401 cggaccacgc tcccgacacc accgacttca gtcgagtggt ccttcaactt ccacctagcc
     2461 ccggccacaa taatacttcg cgacaccacc gacttcagtc ggtggtgtca ggaggggttg
     2521 aagactgtgg gactgttgtt ggcttttcgc tcgcggccgg tattgagcga cgcgcgcggc
     2581 atgctaatat cctgaccaca tgaaggtagg ttcagttctg ccgtctttcg aggacgccag
     2641 cgaatggctg aatcgaatga cgccgaatcc tcgagaaatc agtggccggc cgacattagt
     2701 tcacttctgg tctatcagta gcgacatcgc cg
//
LOCUS       DASSFP010000049         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1947028, whole genome shotgun sequence.
ACCESSION   DASSFP010000049 DASSFP010000000
VERSION     DASSFP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947028"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFH91_01035"
     CDS             <1..1176
                     /locus_tag="VFH91_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET6861589.1"
                     /translation="REMLREIDKQILPDGADFEASTGYHRLILELLLYTFILCRENDV
                     EIPEQYWTKLHEMLVFARVYLRPDRLAPLIGDSDSGQVLPFKRRRADDHAYLLGIGSV
                     VFKDSRLRLSEESAPEELQWILGDQGVEAFTSMTPNLELETSRAFADAGLYIMRDRDL
                     YLCFNASGAGVHGRGSHGHNDALSIEVSAFGTSFISDPGTFVYGANPEARHAFRSTRY
                     HSTVQIDGHEQNTTDLKVPFVIGNEAQPKVLRWESNQSTDIVVAEHYGYRRFDSPVTH
                     RRTVTFDKKQRAWSLLDEFTGEGEHEFEVRLHFRPELEISVRNSTVIAKSSNGKSLQI
                     SSLSALPAAELEELVSSRDYGEKRPSVSACWRIRGRAGRFEWRITPVDGLASITTRN"
     gene            1177..3102
                     /gene="asnB"
                     /locus_tag="VFH91_01040"
     CDS             1177..3102
                     /gene="asnB"
                     /locus_tag="VFH91_01040"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6861590.1"
                     /translation="MCGICGVWEYQASAGRVDTPLIARMRDQMIHRGPDDAGELIFDE
                     RRGGLGFRRLSIIDLSAAGHQPMHGCDQQLWLVFNGEIYNHARLRTGLKERGHHYASL
                     TDSETILHLYEEKGVEFVNEIEGDFAIALWDERSQQLVLARDRVGVKPLYFYQKDGRF
                     IFASEIKAILQHPAVSPDIDEQALYHYLTFMTTPAPSTLFKDVHKIPAGHVLVVKRDG
                     SLNMNRYWDALPPETTTVRSAEEHQKEILKLLRESISKRMMSDVPFGVFLSGGVDSSA
                     NVALMSEQMSRPVETFTVGFSDAEYLNELDSARRISKQFGTNHHEIIISEKEMQQFLP
                     DLVFHQDEPIADPVCIPLYYVSKLARDSGTIVVQVGEGSDEIFSGYESYVKHLQIYER
                     FWQHAQKVPASVRRAFSDVTVPILAATGKKRTAVELLRRLGADEPMFWGGVVIYDELL
                     KPLVLSKEMKSRLNGTSSLQVVNNYLSRIESERPDSDFLSRLIYLELKLRLPELLLMR
                     VDKITMATSVEARVPFLDHNLIEYVLGIPREVKVVGSSGKHILKQALESVLPHDLLYS
                     RKRGFGAPVREWFRQELGPWFDSHLMNSSLKRRDLLDYNFIGHMLDQHRRGVRDWGFQ
                     LWGLLNLSLWYERWIDK"
     gene            3109..>4428
                     /locus_tag="VFH91_01045"
     CDS             3109..>4428
                     /locus_tag="VFH91_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6861591.1"
                     /translation="MAKKSRTKSTIKANSRRPFSHSVALTLLTRVLMLAGALGTSVLV
                     ARWLGTIGVGSLAVLSVTVALAVQLGCAGLPSANTYFISRDKTKLARVWSNALLFAML
                     AGSVIALLVILSARFKPSLFGQVPLELVTLAAASIPFQLINLLGLNVLLGIERVTQFN
                     LLDALSQLLLLVNAIVAFTLGVDLFVLVLFNTVTAVLVSLIVVAIIFRDIGKISTDVL
                     ALDAQLFKRMIGYGLKFYISVVAGIIILRVDLLLVNKLRGAGEAGVYAVAAQMSTLLI
                     LLPSVIATLLFPRVSAVQDVEGDLTMRATRHTAFVMFIVCLIAVPFAFALPYVYGQSF
                     RGSTLQLLILLPGVLLLGIESVMVQHFNAQGLPFTIPVFWIICVVANIGLNLIAIPRF
                     GANGAAVVSTITYALIFVLVATYFRTKTGNTLSTALLLRQGEFRQLFS"
BASE COUNT         1033 a         1048 c         1167 g         1180 t
ORIGIN      
        1 cgcgaaatgc tgcgtgaaat agacaaacag atcttgcctg acggggcgga ctttgaagcc
       61 tcaaccggtt accatcgatt aattctcgag ttgctcctct atacttttat cctgtgtaga
      121 gaaaatgacg ttgagatccc cgagcagtac tggaccaaat tgcatgagat gcttgtattc
      181 gcgagagttt atttgcgacc agacaggtta gctccgctta tcggcgacag tgacagcgga
      241 caagtcttgc cgtttaaacg aagacgggcg gatgatcatg cttacttgtt ggggatcggt
      301 tcagttgtgt ttaaggattc tcgacttaga ctttctgagg aaagcgcgcc tgaagagttg
      361 cagtggatac tgggagatca aggtgtcgag gcgtttacgt cgatgacgcc taaccttgag
      421 cttgagactt cgagagcgtt tgctgatgcc ggactctaca tcatgcgaga tcgagactta
      481 tatctttgtt tcaacgccag tggggccggg gtacatggac gcggctcgca tggacacaac
      541 gatgcgttga gtatagaagt ctccgctttc ggaacatctt ttatttctga tccaggtact
      601 tttgtttacg gcgcaaatcc ggaagcacgt cacgcatttc gctcgacacg ttaccattcg
      661 acggttcaga ttgacggcca cgagcagaac acgactgact tgaaggttcc cttcgttatc
      721 ggcaatgaag ctcagccgaa agtattgcgt tgggagagta atcagagtac ggacatcgtg
      781 gtcgcggaac attacggcta cagacggttc gattcgccgg taacccaccg gcgaaccgta
      841 acattcgaca aaaagcaacg agcctggagt ttgctcgatg aattcacggg agaaggtgaa
      901 catgagttcg aagtacgatt gcactttcgt ccggaactgg aaatcagcgt acgcaactcg
      961 acggtgatcg ctaaaagcag caatggaaag tcgttacaga tatcctcgct aagtgcttta
     1021 cctgccgctg agttagaaga gctcgtaagt tcgagagatt atggagaaaa gagaccttct
     1081 gtgtccgcct gctggcgtat tcgaggaagg gcaggccgct tcgaatggag aatcactcca
     1141 gtcgatggct tagcatccat tacgacgaga aattagatgt gcggcatttg tggagtttgg
     1201 gaataccagg caagcgcggg gcgagttgat acccctctga ttgcgcgtat gcgtgatcaa
     1261 atgatccatc gtggtccaga tgatgccggt gagctcatct ttgatgaacg tcgtggaggc
     1321 ttgggctttc gaagattgtc aattatagat ttgtcggctg cgggccatca gcccatgcat
     1381 gggtgcgacc aacagttatg gcttgtcttt aatggagaga tctataacca cgcgcgtttg
     1441 cgcacagggt taaaagagcg cggtcaccat tatgcttccc tcactgattc agaaaccatc
     1501 cttcacctgt atgaggaaaa aggagtcgag tttgttaacg agattgaggg agattttgcg
     1561 atagccttgt gggacgagag gtctcagcaa ctcgtcctgg ctcgcgatcg cgttggggtg
     1621 aaaccacttt acttctatca gaaagatggg cggtttattt ttgcctcaga aatcaaggcc
     1681 atcctccaac atcccgccgt ttctcctgat atcgacgaac aagcgcttta tcactatctg
     1741 actttcatga cgacgccggc gccgagcact ctatttaagg acgtgcacaa aattccggct
     1801 ggtcatgtgc tggtggttaa acgagatggt tccttgaaca tgaaccgcta ctgggatgct
     1861 ttgcctcccg aaacaactac ggtcagaagt gcagaggaac atcagaagga gatcctcaaa
     1921 ctcttaagag agtcgatcag caagcggatg atgtcggatg ttccgttcgg cgttttctta
     1981 tccggaggag ttgattcttc agctaatgtc gcgctgatgt ccgaacagat gtcgcggccc
     2041 gtcgaaacgt ttacagttgg ttttagtgac gctgaatacc tcaacgaact ggattcggcc
     2101 cgacgtatct caaagcagtt tgggaccaac catcacgaga tcatcatctc agagaaggag
     2161 atgcaacagt ttctcccgga tctcgtcttt caccaggacg agcccatagc agatccggtc
     2221 tgtattcctc tctactacgt atcgaaactt gcaagagact caggaacgat cgttgttcaa
     2281 gttggcgaag gatcagatga gatctttagt ggctacgaga gctatgtaaa gcatctgcag
     2341 atctacgaaa ggttctggca gcacgcgcaa aaggtgcccg cctcagtgcg tcgagcgttt
     2401 tctgacgtaa cggttccgat acttgcagca accggaaaaa agcggactgc ggttgaactc
     2461 ttgcgtcgat taggagctga tgaaccgatg ttttggggcg gtgtcgtgat ttacgacgaa
     2521 ctattaaaac ctcttgtatt gtccaaagag atgaagagcc gcctcaatgg cacttcttca
     2581 ttgcaagtcg tcaacaacta tctctcgcgg atcgagtcag aacgacccga ttcagatttt
     2641 ctcagtcgcc tgatttatct tgaattgaaa ctgcgtttac cagagctgct gctgatgcgc
     2701 gtcgataaga tcacaatggc tacgagtgtg gaagccaggg tcccgtttct tgatcacaac
     2761 ctgattgaat acgtgcttgg tataccgcgc gaagtgaagg tggttggtag cagcggcaag
     2821 cacattctta aacaagcatt ggaaagcgtt ttgccgcatg atcttctcta cagtcgaaag
     2881 cgaggcttcg gtgcgccggt ccgcgagtgg ttccggcaag aactgggtcc atggtttgac
     2941 tcgcatctca tgaactccag cttgaagcgg cgcgacttgc ttgattacaa ctttatcggc
     3001 catatgttag accagcacag gcgaggcgtc cgtgattggg ggtttcaact ttgggggctt
     3061 ttgaatctaa gtctgtggta tgaacgttgg atagataaat agatactcgt ggccaagaaa
     3121 tcccgcacga agtcgactat aaaagcgaac tccaggcgcc cgttcagtca cagcgttgcg
     3181 ttgacgctac ttacacgagt gttgatgctg gccggcgcgc tcggcaccag cgtcttagtt
     3241 gcaagatggt tgggcacgat cggcgtgggt tcccttgccg tgcttagcgt gacagttgca
     3301 ctggcagtgc aattaggttg cgccgggttg ccatccgcca atacatattt tatttcccgg
     3361 gacaaaacta agctcgcgcg cgtgtggtcc aacgcgttat tgtttgcgat gcttgcagga
     3421 tcggtaatcg ctcttctggt aattctttca gcaaggttta agccttcact gtttggtcag
     3481 gttccccttg aattagtaac actcgccgca gcctccatcc cgtttcagtt aattaactta
     3541 cttgggctta atgttctcct tggaattgag cgagtgacgc aattcaatct tctggacgcg
     3601 ttatcgcagt tgttattact tgttaatgca atcgtggctt ttaccctggg agtcgatctg
     3661 ttcgtgcttg tacttttcaa cactgtaacc gccgtgctcg tgagcctaat agtagtggcg
     3721 atcatctttc gagatatcgg caaaatcagt acggacgtac tggcactcga tgctcaactg
     3781 tttaagcgga tgattggcta cggtctcaaa ttctatattt cagttgtagc cggaattatt
     3841 atcttgcgag ttgatctgct actcgtaaat aagctccgag gcgccggcga agcgggcgta
     3901 tatgcagtgg cagctcaaat gagcactcta ctgatcctgc taccctccgt tatcgcaacg
     3961 ctgctcttcc ctcgtgtttc tgccgttcag gacgtggaag gcgatttgac gatgcgagcc
     4021 acacgacata ccgcctttgt aatgtttatc gtgtgtctaa ttgctgtccc gtttgctttc
     4081 gcgctcccat acgtttatgg acaatccttt cgaggttcga cgcttcagtt gttgatcctg
     4141 ctgcccggtg ttctgttgct cggaatcgaa tccgtaatgg tccagcactt caacgcacag
     4201 ggactgccct ttactattcc agtgttctgg ataatttgcg ttgtagcaaa tatcggcctc
     4261 aacctgattg cgatccccag gttcggtgcg aatggggcgg cagtggtttc gacaatcact
     4321 tacgctctta tttttgtgct tgtagcgacc tactttcgca ccaagacggg caacactctt
     4381 tcgactgcat tactgttgcg gcaaggcgag ttccggcaac tgttcagt
//
LOCUS       DASSFP010000050         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_904928, whole genome shotgun sequence.
ACCESSION   DASSFP010000050 DASSFP010000000
VERSION     DASSFP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904928"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VFH91_01050"
     CDS             complement(<1..933)
                     /locus_tag="VFH91_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET6861592.1"
                     /translation="MEAIATLMFFMFVIFAIIVMLVIGVKYVRIVPHATVLVIERLGK
                     FHKLADSGLHILTPFLDKPRGVFWTNTRPGLTMIDLREQYIDLPPQPVITRDNVTIHV
                     DSVVYWQITDAVKAIYEMNDLVGGLVQLTITGMRAVMGDMDLDHTLSQRDQINSKLRI
                     ILDEATDKWGVKVTRVDVKNINPPEDVRITMEKQMTAERNRRALVLQAEGDRQAAITR
                     AEGEKQAAVTRAEGEKQSSILAAEGAAQARLRNAEAEAEAIARVADAMQAHGNPAQYL
                     ITTRYIESLRDMTRTNNSKVIFMPVETSALLSSVG"
     gene            complement(1033..2391)
                     /locus_tag="VFH91_01055"
     CDS             complement(1033..2391)
                     /locus_tag="VFH91_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6861593.1"
                     /translation="MTNLNQLSLTELTTVIRNKQVSSLEVVQSFIAQVEALNSNLNAI
                     VTLNDRLLDHARLSDAQLAKGEIAGPLHGIPITVKDTIETEGLRTTSGSLIRVDYVPT
                     TDAPAVEMLKQAGAIILGKTNAAEMAMDYNADNPVFGRTYHPLDKRFTPGGSSGGEAV
                     AIATHMSPAGIGSDLAGSIRIPAHFCGIAGLKPGTNTIPGAGQYPPSTGPYSLGAVIG
                     PMACNVADLELMFQALTPSSRMANKGFDTNIRIAWYATDGAIPVTDATEKAMTVAAQI
                     LSEQGFDCREELAPHVQFGNELWLKLFSRASVVQLRKAYAGREADGGSFVSWRLRNAD
                     TPPPPTLDEYISDWMERDRLREELLKWMETTPVILSPVGATPAFEHDTLKLRVRNKEI
                     GTFKAFGYSQAFNVFDLPVVTVPVSKSEEGLPIGIQIVGRPNEERTILRIARLLENID
                     EK"
     gene            2637..3311
                     /locus_tag="VFH91_01060"
     CDS             2637..3311
                     /locus_tag="VFH91_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HET6861594.1"
                     /translation="MAIRLSRTFILRKLHQLSGIVPLGLFLLEHFYTNSKALDGAKHF
                     NDAVQDLQSIPYILLVEIGGIFIPLIYHAVYGLVITVEARPNNLNYPYPRNWFYLIQR
                     ITGVILFFFIAFHVLNFRFGMIPGLNTISVAEHPAESFNIVSREFSMIPIFIIYMIGI
                     TATVWHLANGIWLFLVDWGITIGERAQRLAGYACIAFGVVLLLVGVNAAVAFIRPGGL
                     LGGLIK"
     gene            3311..>4837
                     /gene="sdhA"
                     /locus_tag="VFH91_01065"
     CDS             3311..>4837
                     /gene="sdhA"
                     /locus_tag="VFH91_01065"
                     /inference="COORDINATES: protein motif:HMM:NF006392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET6861595.1"
                     /translation="MASNGMKIAIVGGGLAGLAAAMKIAEEGHAVDLFSVVPVKRSHS
                     VCAQGGINGAVNTKGEGDSTWEHFDDTVYGGDFLANQPPAKLMCDMAPSIIYLFDRMG
                     VPFSRTNEGLLDFRRFGGTKHHRTAFAGASTGQQLLYALDEQVRRFEVSGKVRKFEHW
                     EMLSLVLDDHQVCRGLIAMDLQTLELKAFPADAVIIATGGPGLIFGKSTNSMVCTGSA
                     VSACYQQGAKYANGEFIQVHPTSIPGEDKLRLMSESARGEGGRVWVPRNAGDTRSPSQ
                     IPENERNYFLEEKYPAYGNLVPRDIATREIFQVCIEGRGIGGQNQVYLDLTHIPAETL
                     DRKLGAILEIYEMFVGDDPRHVPMRIFPGVHYSMGGLWVDFNQRTNIPGLLAAGECDY
                     SIHGANRLGANSLVSCVYGGFVAAPAAIEYAKNVERNGADGSRIYDQELQHQKEINDN
                     LIKQSGNENQYILHNEMGKWMTDNVTVVRYNDKLKATDEKLLELMDRYKRISINDSNL
                     W"
BASE COUNT         1146 a         1226 c         1200 g         1265 t
ORIGIN      
        1 gccaacactc gaaagtagcg ccgacgtttc caccggcata aatatgactt tggaattgtt
       61 cgtcctggtc atatcacgaa gactttcgat gtaacgggtt gtgatcaggt attgggccgg
      121 gttgccgtgg gcctgcatcg catcggccac tcgcgctata gcttctgctt ccgcttccgc
      181 attgcgcaaa cgagcctggg ccgcaccttc cgccgcaagg attgacgact gtttttctcc
      241 ctcagctcgg gtaactgccg cctgcttttc accttccgct ctggtgattg cagcttgacg
      301 atcaccttct gcctgcaaaa cgagagcgcg gcggtttcgt tcggccgtca tctgtttctc
      361 catcgtaatt cgcacatctt caggcggatt gatgttcttg acgtcaacgc gcgtgacttt
      421 tacgccccat ttatcggtag cttcgtcaag gatgatgcga agtttcgagt tgatctggtc
      481 gcgttgcgaa agcgtgtggt ccaaatccat atcacccatc accgcgcgca ttcccgtgat
      541 cgtcaattgc accagaccgc caaccagatc attcatctcg tagattgcct tcacggcatc
      601 ggttatttgc cagtaaacga cagagtcgac gtgaatggtc acattatcgc gagttatcac
      661 tggctgtgga ggcaggtcga tgtactgctc tcttaaatcg atcatcgtca agccaggtcg
      721 agtgtttgtc caaaatacgc ctcgtggttt gtcgaggaac ggagtcaaga tatggagccc
      781 actgtcagca agtttgtgaa acttacctaa tcgctcaatc acaagtaccg tcgcgtgagg
      841 cacaattcgc acatacttca ctccaatcac gagcatcacg attatcgcga agataacgaa
      901 catgaaaaac atcaaagttg cgatggcttc catacggact cctgttcagc tgggggtttg
      961 ggtcaattgt tagctcgtct gagactatag cgaataggtt gaggccaatt caaattttta
     1021 gatcactccc gatcattttt catcgatgtt ttccagcaat cgtgcaattc ttaagatagt
     1081 tcgttcttcg ttgggtcggc ccacaatctg aataccaatg ggcaagcctt cttctgactt
     1141 actaactgga acagtcacta cgggcaagtc aaagacattg aatgcctgcg aatagccaaa
     1201 tgctttgaag gttccgattt ccttgttccg cactctgagt ttcaaagtat cgtgctcaaa
     1261 agcgggagtc gcaccaaccg gagagaggat gaccggcgtc gtctccatcc atttcaataa
     1321 ctcttctcga agccgatcgc gctccatcca gtctgaaata tattcgtcca gagtcggtgg
     1381 aggcggagtg tctgcatttc tcaatcgcca actcacaaag gaaccaccat cggcttccct
     1441 tcccgcgtaa gcctttcgga gctggaccac cgaagcgcgg gagaacaact tcagccacaa
     1501 ttcatttcca aactgaacat gaggcgccaa ctcttcccgg cagtcgaatc cttgctccga
     1561 caaaatttga gcagccacag tcatagcctt ttcggtagcg tccgttaccg gaattgctcc
     1621 atctgtcgcg taccacgcaa ttcgaatatt agtgtcgaat cctttattgg ccatacgact
     1681 cgagggagtg agagcttgaa acatcaactc caggtcagcc acgttacaag ccattggtcc
     1741 aatcactgcc cccaatgagt atggtccagt gctgggagga tactggcccg cccccggaat
     1801 cgtgttcgtt cctggcttta atccggcaat gccacaaaag tgggccggga tgcgaatgga
     1861 tccggctaag tcgctcccaa taccggctgg cgacatgtgg gtagcgatgg cgacggcctc
     1921 tcctccactt gaaccgccgg gtgtgaaccg cttatcaagc ggatgataag ttctaccgaa
     1981 aacgggattg tcagcgttgt agtccattgc catttccgcc gcattggttt ttccgagaat
     2041 gattgcccct gcttgtttca gcatttcgac ggctggagca tctgtcgttg gaacgtagtc
     2101 gacgcggatc agtgagccac ttgtggtccg taaaccctca gtttctattg tgtctttaac
     2161 tgtgatgggt attccatgaa gcggacccgc aatttctcct ttagcgagct gagcatcaga
     2221 cagcctggca tggtccagca aacggtcgtt caatgtaact atcgcgttta ggttcgaatt
     2281 tagagcttca acctgagcga tgaatgactg cacaacctca agtgacgaga cttgtttgtt
     2341 gcggatgact gtggtgagtt cggttagaga tagctggttt agatttgtca taacgaagtg
     2401 ctttggtttg tactttgcgt tttgcgcttt gtactttgtg gtttgtcgtt cagaccgcga
     2461 tcgaaaacgc tcaaaatcca aaggacaaag tacaaaggac aaagagccga aaggctgttt
     2521 tgcttttcgc tcacttattc ctataatcca agtgctctgt gtagaatcat ttacaacctg
     2581 cctaatcttg gtcaactggc cttgaggttc aagtttaatg gcaaaggagt gaattcttgg
     2641 ctattcgttt gagcaggacg tttatcctgc gcaagctaca ccagctttcc ggtatcgtcc
     2701 cactcggtct ttttctctta gaacactttt acactaactc aaaggcgctc gatggggcga
     2761 agcactttaa cgatgccgtt caggatctgc agtcgattcc atacatcctg ttagtagaga
     2821 tcggcggcat ctttataccg ctcatctatc acgcagtcta tggattggtg attacggttg
     2881 aagccaggcc caacaacctg aactacccct acccccgcaa ctggttctac ctgatccagc
     2941 gcatcacggg tgtcattctc ttcttcttca ttgcatttca tgttttgaac tttagatttg
     3001 gaatgattcc cggtttgaac accatttcgg tggctgagca ccccgcagag tctttcaaca
     3061 ttgtctcgcg tgagttcagc atgattccta tctttatcat ctacatgatc ggaattaccg
     3121 cgacggtgtg gcaccttgct aacggcattt ggctattctt agtagactgg ggaatcacaa
     3181 ttggtgagcg cgcccaacga ttagccggtt atgcatgtat cgcatttggc gtcgtattgc
     3241 tgttggtcgg cgtgaacgcg gcagttgcat tcattcgtcc aggtggattg ttaggaggcc
     3301 tgattaagta atggcaagta acggtatgaa aatagcgatc gtaggtggtg gactggccgg
     3361 tttagcggct gccatgaaga tcgcagaaga gggacatgca gtcgatcttt tttcagtcgt
     3421 tccagtgaag cgttcgcatt cagtctgcgc tcagggggga attaacggcg ccgtcaatac
     3481 aaaaggtgag ggcgattcca cctgggaaca cttcgacgac actgtttacg gcggagactt
     3541 cctcgccaat caaccacccg ccaaattgat gtgtgatatg gcgccatcga tcatttatct
     3601 ctttgatcga atgggtgtcc cattttcccg caccaacgaa gggttgctgg acttccggcg
     3661 ttttggaggt accaagcacc accgtactgc ttttgcaggt gcttcgaccg gacaacaatt
     3721 actttacgct ctcgatgaac aggtccgacg ttttgaagtg tcaggtaagg taagaaagtt
     3781 tgaacattgg gaaatgctct cgctggtcct tgatgaccat caggtttgtc gcgggctaat
     3841 tgcgatggac ctgcaaacgc tcgaactgaa ggccttcccc gcagacgcag tcataattgc
     3901 cactggcggt ccgggattaa tttttggtaa gtcgacgaac tcgatggttt gcaccggcag
     3961 tgcagtctct gcctgctatc aacagggtgc gaaatacgcc aacggtgaat ttattcaggt
     4021 ccatcccaca tccattcccg gcgaagacaa gttgcgactg atgtctgaat cggcgcgcgg
     4081 tgaaggagga cgcgtttggg ttcctcgcaa cgcaggtgac acaaggtcgc catcgcaaat
     4141 acccgagaat gaacgcaact atttcctcga agagaaatat ccggcttatg gaaatctggt
     4201 tccgagagac atcgcaacac gcgaaatctt tcaagtctgc attgaaggac gcggtatcgg
     4261 cggtcagaac caggtttatc tcgacctgac tcacattccg gcagaaaccc tggatcgaaa
     4321 gcttggagcc attctcgaaa tctacgagat gttcgttggt gacgatccac gtcacgtgcc
     4381 gatgcgcatt ttcccgggcg tccattactc aatgggtggc ctatgggtcg atttcaatca
     4441 gcgtactaac attcccggtc tgttggccgc cggtgagtgc gactattcta tccatggcgc
     4501 aaatcgatta ggtgcaaact cactggtcag ctgtgtctat ggcggatttg ttgccgcgcc
     4561 agccgcgatc gagtacgcaa agaatgtcga acgtaatgga gcagacggtt cgcgtatcta
     4621 cgatcaggaa ctacaacatc aaaaagagat caacgacaac ctcattaagc aaagcggcaa
     4681 cgagaatcag tacatactgc acaacgagat gggcaagtgg atgactgaca atgtcacggt
     4741 tgtccgttac aacgataaac tgaaagcgac ggacgaaaag ctactcgagt tgatggaccg
     4801 atataaacga atttccatca acgattcaaa cctttgg
//
LOCUS       DASSFP010000051         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_905131, whole genome shotgun sequence.
ACCESSION   DASSFP010000051 DASSFP010000000
VERSION     DASSFP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905131"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..941)
                     /locus_tag="VFH91_01070"
     CDS             complement(93..941)
                     /locus_tag="VFH91_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6861596.1"
                     /translation="MSFITTKDGTQIYYKDWGTGQPVVFSHGWPLTADAWEDQMLFLG
                     NNGYRCIAHDRRGHGRSSQPWDGNEMDTYADDLATLVETLDLHDAIHVGHSTGGGEVA
                     RYIGRHGTKRVAMLVLIGAVPPIMLKTEANPGGQPIEVFDQMRAAVQSDRAQFFKDLA
                     EQFYGANRPNAKVSQGLKDSFWLQSMMGSHEAIYECIKAFSETDFTEDLKKFDVPTLF
                     IHGDDDQIVPFADAAPLAAKLVKGSTLKVYEGAPHGLCSTLKDRVNADLLAFFKSSQR
                     SSASSA"
     gene            complement(992..1438)
                     /locus_tag="VFH91_01075"
     CDS             complement(992..1438)
                     /locus_tag="VFH91_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861597.1"
                     /translation="MGLSLEESEPDHDSLEPSLQRLFSSFPNGAPGFALFFLRALVSI
                     TLFVQAANYLRTPEINLSGWIVMSAMAIVGLCFALGFMTPLMSSVLVLGALAIGLSWV
                     PSPSQNLLDTQFSIVNLVVVAAAIGALGPGAFSLDARMFGRREIRI"
     gene            complement(1592..2221)
                     /locus_tag="VFH91_01080"
     CDS             complement(1592..2221)
                     /locus_tag="VFH91_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6861598.1"
                     /translation="MSDVKTIRVLSADDHPLLREGIATLINSQPDMKLIAEAANGQEA
                     IQQFDEHRPDVTLMDLRLPDMSGIDVVIAIRSQHPEARIIMLTTFEGDVEIQRALEAG
                     ARGYLLKSMPPKDMLEAIRQVHAGKKRIPAEIASHLAEHMGDEALTEREIEVLRAIAE
                     GNRNRDIANKLFISEETVKVHIKHIMEKLNASDRTQAVAIGVRRGIIEL"
     gene            complement(2218..3444)
                     /locus_tag="VFH91_01085"
     CDS             complement(2218..3444)
                     /locus_tag="VFH91_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6861599.1"
                     /translation="MVQLALLALLPWSIHSSAQQPDSLPAIEFTQVPAADRGGQETLS
                     TISGRVIGGNGERQRIVIYARSGSWFVQPFTDQPFTRISPNGSWSNTTHLGTEYAALL
                     VEAEFHPPERTNVLPPVGNGVLAVSVVPGSPLFWQTWWFWTSIVLASLGSIFVYYRIR
                     QQRLISQMNIRFEERLADRTRIAQDLHDTLLQGLLSASIQLHVADELLDSSSPAKPLL
                     GRVLQMMGQVVEEGRNAVQGLRSTSSDTRDLERALLQVRKECVGENKPEFKVVVEGSL
                     RPLHPLIRDEVYRVGREALVNAFRHSNANRIEVELEYGVKEFRMLVRDDGVGIDSYIV
                     NQGREGHWGLSGMRERTTEIGGKLKVLSRAGAGTEVALSIPADIAFERVEQKSGIMQL
                     LRLDRLRLVNGKDKTK"
BASE COUNT          935 a          970 c          892 g          919 t
ORIGIN      
        1 acaaaggttg cacagagtaa gccaagttag gacactagcg acgctactcc gcgattcgcg
       61 actgaccagt ggctaccatg tgattgactg ctctaggcag agctggcact ggaccgttgg
      121 gaagacttga agaaggccaa caagtctgca ttaacccgat ccttcagcgt ggaacacaga
      181 ccgtgtggcg caccctcata aacttttaac gttgagcctt tcaccaattt cgctgcgagt
      241 ggcgccgcat ctgcaaaagg aacgatttga tcgtcgtcgc cgtgaatgaa taatgttggc
      301 acgtcgaatt tcttgaggtc ttccgtaaag tctgtctccg agaacgcttt gatacactcg
      361 tagatcgcct cgtggcttcc catcattgac tggagccaga aagagtcttt cagaccttgc
      421 gagactttcg cattgggccg gtttgctcca tagaactgtt cagctagatc tttaaaaaac
      481 tgtgcccggt cactttgtac tgcagcacgc atctgatcga acacttcgat tggctgtccg
      541 ccgggattgg cttcggtttt caacataata ggcggtacag ctccaatcaa aactaacatc
      601 gcgacacgtt tcgttccgtg gcgtccgatg taacgagcga cttcaccgcc gccggttgaa
      661 tgacccacgt gaatagcgtc atgcaggtcc aacgtctcaa ccagggtggc gagatcgtca
      721 gcatacgtgt ccatttcatt cccatcccag ggctgacttg agcgaccgtg tccacgacgg
      781 tcatgagcga tgcatcggta accattgttc cctagaaaga gcatttgatc ttcccaagca
      841 tcagctgtaa gtggccaacc atgactgaaa acaaccggct gtccagtgcc ccagtccttg
      901 tagtaaatct gagtaccgtc tttcgtagtt atgaatgaca tgaactttct cctatttctc
      961 ttttgcaggc cgattcgagg aactgattta gttaaatcct gatttctctt cggccaaaca
     1021 tccgcgcatc cagcgaaaac gctcccggcc ctaatgcgcc aatggccgcg gcgactacaa
     1081 ccaaattgac aatcgaaaac tgcgtatcaa gcaggttttg acttggagag ggaacccacg
     1141 acaggccaat agcaagcgct cctaaaacga gaactgacga catcagcggc gtcataaagc
     1201 ccaatgcaaa acataaaccg acaatcgcca tggcagacat gacgatccat ccggataaat
     1261 tgatttcagg cgttctcaag taatttgctg cttgtacaaa tagagttatg cttaccaaag
     1321 ctcgcaggaa aaacagtgcg aaacccggcg caccatttgg gaaactggaa aataatcttt
     1381 gcagacttgg ctccaaacta tcatgatctg gttcagactc ttccaaggat aatcccatta
     1441 cgaaccactt tggatcagta gatcaaggta cctttgtata actccttagt ggtatgccgt
     1501 cgcggtaaag attgaaatta tggggatggt aatttcggag gctgctagtg cctttttggg
     1561 agcaattcga acgctaaaat caaaaacggg tctacagttc aattatgccg cgacgcactc
     1621 cgatggccac cgcctgagtc ctatcggaag cgtttaactt ctccatgatg tgtttgatat
     1681 ggaccttaac agtttcttcg gagatgaaaa gcttgttggc gatatcgcgg ttgcgatttc
     1741 cttctgcaat agcgcgcaag acctcaatct ctctttccgt caaagcctcg tcgcccatgt
     1801 gttcggctaa gtgtgacgct atctctgcag ggattcgttt cttgcctgca tggacctggc
     1861 gaatagcctc aagcatatct tttggtggca tactctttag caaatagcca cgtgctcctg
     1921 cttccagtgc ccgctgaatc tcaacgtcac cttcaaacgt agttaacatg attattcgcg
     1981 cttccggatg ttgcgaccgg atcgcaatta cgacatcgat gccactcatg tcaggaagtc
     2041 gaagatccat caaagtcacg tcgggccggt gctcgtcaaa ctgttgaatc gcctcctggc
     2101 catttgctgc ctcagcgatc agcttcatat ctggctggct attgatcagc gtagcgatgc
     2161 cttctcgcaa caaggggtga tcatcggcgc ttagaactcg aatcgttttg acatcgctca
     2221 tttggttttg tcctttccgt ttactaaccg caaacgatca agtctcagca actgcatgat
     2281 tcccgatttt tgttcaacgc gctcaaacgc aatgtcagcc gggatcgaaa gtgccacttc
     2341 agtcccggcg ccagcccgac tcaagacttt caacttgcca cctatttcag tggtacgttc
     2401 gcgcatgccg gacaagcccc aatggccctc gcgaccctgg ttcacgatgt aagaatcaat
     2461 accaactcca tcatcacgta ccaacatccg aaattctttg acgccatact caagttcaac
     2521 ttcaattcga ttcgcattag agtggcgaaa agcattgact aaagcttcgc gaccgactcg
     2581 atacacttcg tcacgaatca ggggatgcaa aggtcgcagg gatccttcaa ctacgacttt
     2641 aaactcgggc ttattctcgc caacgcattc cttcctgacc tgaagcagcg cgcgctcgag
     2701 atcgcgtgta tcgctgctgg tggatctgag tccctgcact gcattgcgac cttcttcaac
     2761 tacctggccc atcatctgca acactcttcc cagcagaggt ttggctggag aggaggaatc
     2821 aagaagttca tccgcaacgt gaagttgtat agaagcgctg agcagtccct gcaacaaagt
     2881 atcgtgcaga tcttgagcga tacgcgttct gtccgcgaga cgctcctcaa atcgaatatt
     2941 catctgcgag atcaaccgtt gctgtcggat gcggtagtaa acaaagatgc tgcccagcga
     3001 tgcgagcacg atgctggtcc agaaccacca cgtctgccag aacaatggag aaccgggaac
     3061 gacagacact gccaatacgc cattaccaac gggaggtaag acgttggtgc gctcgggagg
     3121 atgaaattct gcttctacca ggagggcggc atactcagta ccaagatgag tagtattgga
     3181 ccacgaccca tttggtgaaa ttcttgtaaa tggttggtcc gtgaagggct gtacaaacca
     3241 cgaaccgctc cgggcataga tcacgattcg ttgccgctcg ccattaccac caattactcg
     3301 tccacttata gtgcttagag tctcctgacc acctcgatcg gcggcgggga cctgagtaaa
     3361 ttcgattgct ggaagtgagt caggttgttg ggcggagcta tgtatggacc aggggagcag
     3421 cgccaatagc gctaactgaa ccaacaagga tcttataaag ggagagactc caattctggg
     3481 actcatgggc gatactattg ttcggttgag atagagtaag cccgttcggt tggctagaca
     3541 agggaggatt atttcgagta gtgatgcagt ttgggatgtc cgatatgctt tagcttgtcg
     3601 gtcgtcgttg actcgctcct ctgaacttga gtgtgcatca cgaccgacaa gctaaaagcc
     3661 tcttgcaaaa gtcagtatca accacaagcg cgttaacagt ttgaagtctt ggagct
//
LOCUS       DASSFP010000052         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1649886, whole genome shotgun sequence.
ACCESSION   DASSFP010000052 DASSFP010000000
VERSION     DASSFP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649886"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFH91_01090"
     CDS             complement(<1..1100)
                     /locus_tag="VFH91_01090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6861600.1"
                     /translation="MVKEINTQIRSDIVFGRELSRQDAEAVHLAEKEPRLETVSVLVP
                     SHNHGRFISRCLRSIIEQSHKPLQLIVIDDGSEDDSVRRIEQELKDCPFESELIASTH
                     KGLVQTLNEGLKRSRGKYFAYLGSDDVWLPGFLASRVKLLESRSDAVLAYGHSFVIND
                     EDQIVECTKDWAAYNTGSAREMLLNVIVPFSPSVLYRREILERCHWNEDAQLEDYDLY
                     LRLSVEGEFAFDEATSCAWRTHGKNSSQDLQFMLKECLEAQRRAASSLHLTSNELEKA
                     NARLKWRFGGDFIKAGERGRGIKLLCLNLKGAPSYASVARMMGAVIIPTPILRWRKKY
                     IEGIVNLISGKDFSARKWPKFSCLSCTTSIFKL"
     gene            complement(1392..3584)
                     /locus_tag="VFH91_01095"
     CDS             complement(1392..3584)
                     /locus_tag="VFH91_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6861601.1"
                     /translation="MTMLSLFGNVRCGLTTVGFVVLLFFTFVLHPTQAQQQSKDPQKT
                     KDEDDVIKVKSNLVNIDVLVKDKKGKYIPDLKVDDFTIFENGVQQKVEFFDAPLSNNE
                     PKRGEVQVEAANLPRHYVSLVLDSQTTDATNLKQVREGIIKYVREQIKDDDAVALFSV
                     TNGLQMLQPFTQDKTTLITALQNAGTGSLSKNFEQKDLRDNIASLRDELTSSPAAGAP
                     ITTTAGGSQAARAMIANRVLQQFIAFRTALNLQQSRPILAALAAICEGLRPLPAKKTL
                     VLFSQGFVTPGVLDWQIQTTVDIANRANVAIYIIDSAGLRAGAPQSGSLVPASPLAGV
                     SAVTDQEQRIKAVGGETVFDNTRTEGQNREYDILYRISGDTGGKFLKGNNDIALGLER
                     INQEIQARYTLAYRTTNQNFDGTFRKVKIQVHRPDAQILSRSGYYAIPPNEIVLLSPA
                     DKKLLSDFASAQANPDLPLFVSLTPFRSQEGLYTVPLAFELPPSAMRFEAKGDRRLMQ
                     LDVLGVLKAGHDKILSRLGGSFDVNLTADEYKAIVSNNIYYRQDLQLTSGEYTLDLIV
                     KDRQSGKIAARREQLVLSEPDSEFAVTPVALSRYVEPTNLLSGSGSLDVFTHDKIKIR
                     PSPSRRFQNTDNLIMLSAIYNAANSPETGKPLVRVSVRLMKDGQPATRPFDYVLTEIQ
                     QDPIPHLTFAEYIRLAGLAPGNYTALIEAKDMVTRKFLKQEAAFEIVK"
BASE COUNT          946 a          957 c          935 g         1047 t
ORIGIN      
        1 agtttgaaaa tggatgtcgt acaagacaag caagagaact tgggccattt tctcgcgcta
       61 aaatcttttc ctgagattaa gttgacaatg ccttcaatat acttctttcg ccaacgcaag
      121 atcggcgtgg gaatgatcac agctcccatc atgcgtgcga cagaagcata tgagggtgca
      181 ccttttaaat tcaagcagag taacttaata cccctgcctc tttctcccgc cttgatgaaa
      241 tcaccgccga accgccactt caatcgtgcg ttcgctttct cgagttcatt tgacgtgagg
      301 tggagactgg aagcagcccg gcgctgcgcc tccaaacact ccttgagcat gaactgtaag
      361 tcctgactgc tgttttttcc atgagtcctc catgcgcatg aggtagcttc gtcaaaggca
      421 aactcacctt caacgctcag gcgaagatag aggtcgtaat cctccagttg agcatcctcg
      481 ttccagtgac accgctcgag tatttcacgg cggtacaaga cggaggggct gaatggaaca
      541 attacgttga gcaacatctc acgcgctgaa ccggtattgt aagcagccca atctttcgta
      601 cattcaacaa tctgatcttc gtcgttgatt acaaaggaat gtccgtaagc caaaacggcg
      661 tcgctacgcg actcaagaag ctttaccctc gacgcaagaa acccaggtag ccacacgtca
      721 tccgagccaa ggtaagcgaa gtattttccg cggctgcgct taaggccttc gttaagagtt
      781 tgcacaagcc ccttatgagt gcttgctatt agttcgcttt caaacgggca gtccttcaac
      841 tcctgttcga tcctgcgtac cgagtcatcc tccgacccgt cgtctatcac aatcaactgt
      901 aggggtttgt ggctttgttc gatgatagaa cgaaggcaac gactgatgaa gcgaccatga
      961 ttgtgtgaag gaactaatac ggaaacggtc tcgagtctcg gttctttttc cgcgaggtgt
     1021 acggcctccg cgtcctggcg actaagttct cttccaaaaa caatgtcgga tcggatctgc
     1081 gtattaatct cttttaccac tacgctgaga ggacctcgag taagagagtt gagaacataa
     1141 atgtctcaat cgtatgaaac ctattgttta ccttgtacga ctttttctta cggtactggt
     1201 tcagtttgct ttcctttgcg ttctgtcgga gttccttatt agagttacac acccaagacg
     1261 cagagaacgc ggagatcacg cagagaaatg ccgagaaatg aaactgagcc ggtacctatc
     1321 ttgaacccag agcaggataa atgactggca ggattccggc aagtcgactt actcgcagca
     1381 gaaaacctcc cctatttcac gatctcaaaa gccgcctctt gcttcaggaa tttgcgagta
     1441 accatgtcct tggcttcgat taatgccgtg taatttccag gcgccaaccc cgccaaacgt
     1501 atgtattcgg caaatgtaag gtgcggtatt gggtcctgct ggatttcagt gaggacataa
     1561 tcgaacggcc ttgttgcagg ttggccatcc ttcatgagcc gcacgctgac tctgaccagt
     1621 ggttttcccg tttcaggact gtttgccgcg ttgtatatcg cagacagcat tatcaagtta
     1681 tcagtgtttt gaaatcggcg gctcggcgaa gggcgaatct tgattttatc gtgcgtaaag
     1741 acatcaagac ttcctgatcc gcttaacagg ttcgttggtt caacgtacct gctcagcgca
     1801 actggagtaa ccgcgaattc tgagtcgggt tccgatagga ccagctgctc acgtcgcgcg
     1861 gcgatttttc ccgactgacg gtctttgacg atcaggtcta aagtgtattc tcctgaagtg
     1921 agctggagat cctggcggta gtagatattg ttgctgacga tcgccttgta ctcgtcggcc
     1981 gtcagattca catcaaagct tcctcccagt cttgagagaa tcttatcgtg cccggctttt
     2041 aatactccaa gtacatctaa ctgcattaag cgcctatcgc ccttggcctc gaacctcatc
     2101 gcagagggcg gaagttcgaa cgccagcgga actgtgtaca gtccttcttg agatctgaaa
     2161 ggagtcaacg aaacgaagag cggcagatct gggtttgcct gggccgaagc aaagtcactc
     2221 agcagcttct tgtcggcagg tgataagaga acaatttcat tgggaggtat ggcgtagtaa
     2281 ccagaccttg aaaggatctg cgcatccggc cggtgaactt ggatcttaac tttacgaaag
     2341 gtgccatcga aattctgatt tgtagtgcga tatgccagcg tgtatcgagc ctgtatttcc
     2401 tgatttattc gctccagtcc cagggcaata tcattgtttc cttttaaaaa tttcccgcca
     2461 gtatcacctg agattctgta caagatatcg tactcgcggt tctgcccctc ggtcctcgtg
     2521 ttgtcaaaaa ctgtctctcc accgactgcc ttgatccgct gctcttgatc tgtgaccgca
     2581 gaaactccgg ccagaggcga ggccggtact agagaacccg actgaggcgc gccagctcgc
     2641 agtccagccg aatcgataat gtagatcgca acattagcgc gattggcaat atcgactgtg
     2701 gtttgtatct gccaatcgag aactccagga gtaacaaatc cctgtgagaa aagaaccaga
     2761 gtctttttgg ctggcaacgg cctaagtccc tcgcatattg cggcgagcgc ggcgagaatc
     2821 ggacgagact gttggaggtt tagagctgtg cgaaatgcga tgaactgttg cagcactcga
     2881 tttgcgatca ttgctctagc cgcttgcgat ccaccggctg tagtcgttat tggtgcccct
     2941 gctgctggcg agctagttaa ttcatcgcga agggaagcga tgttgtctcg aagatctttt
     3001 tgttcgaagt ttttagaaag agagcctgtt cctgcatttt gcagtgcggt tatgagtgtt
     3061 gtcttgtcct gagtgaatgg ctggagcatt tgcagtccat ttgttacaga gaatagtgca
     3121 acagcatcgt catcctttat ctgctcgcga acgtacttga tgatgccctc gcgcacctgc
     3181 tttaagttcg ttgcgtctgt cgtttgcgaa tcgagcacga gtgatacgta gtgtcgcgga
     3241 aggttagctg cttctacctg gacctcgccg cgtttaggtt cattattcga tagaggagca
     3301 tcgaagaatt cgaccttctg ttgtacaccg ttttcgaata tggtgaagtc gtcaacttta
     3361 agatccggaa tgtattttcc tttcttatct tttacgagca cgtcgatatt gaccaaattt
     3421 gatttgacct taattacgtc atcttcatct ttagttttct gtggatcctt actctgttgt
     3481 tgcgcctgag ttggatgtaa cacgaacgtg aaaaacaaga gtacaacgaa tccaacagta
     3541 gtgagaccgc accgcacatt accgaaaagg gaaagcatag tcatgttatt caccttaaga
     3601 ttaaagacag cgtgttggtt tccggagtgt atcgatcagt cggcagctca attcctaccg
     3661 ctcaatcaag tgactatcgt cgatcaaaat cgatagctga tcaatatccc acagaagaac
     3721 gatccacgaa aattacacga attaacacta acactaacga agccccatgg cacactcgag
     3781 ttttgacgca gtctttaaag cccggtggtg ccggagtttc acacagtctc tgaagccggt
     3841 ggtttctttc ttcttctgct aaaatcctat cgaatgcgtg aatgc
//
LOCUS       DASSFP010000053         5225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_12008, whole genome shotgun sequence.
ACCESSION   DASSFP010000053 DASSFP010000000
VERSION     DASSFP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12008"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFH91_01100"
     CDS             <1..1067
                     /locus_tag="VFH91_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861602.1"
                     /translation="SANGKTLTVSSVGMNATAQNGGIEYANGPQARTIFSFQIDGLNQ
                     SIAFSPLANKTFGAAPFSVSATASSGLPVSFAASGNCTVAGASVTLTGAGSCTITASQ
                     AGDSNFSPALDVSQTFSIAKAPVTATAGSGLAIYDGFTKTPPACTVTGAYVGSLGCVN
                     SPVSVGPTPGTTQISPIVSGDTLSNFDVTPVNGSFTIGSLKVSTDGIRTELNDALATA
                     TNRRDITRLKDAISSLEQAVSPGRWTADGGQLVCNGGSKVFGSDQDAILQLMAMIRDT
                     TPGIPDATIQRWINILVAIDRNLAQTAITAASSSGVSAKKIADALDELANGDADAARG
                     DYDKAIGHYQTAWNRVTSCN"
     gene            1457..2326
                     /locus_tag="VFH91_01105"
     CDS             1457..2326
                     /locus_tag="VFH91_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6861603.1"
                     /translation="MLDMNWVQDQTNGNGVKINPSSSKGDYVLATGSAAANRLRILHN
                     IYGPGARSLLVRSGLRQGMRVADLGCGVGMVTQMLAELVGPTGEVIGVDFSKAQVEQA
                     KALLPAGITNVEFIEASATDTGLARESFDFVYCRFLLIHLPDPRAALREMYQLLKPNG
                     RLVCEDGDLTSAGSEPPSQLQAFATLFGNLGPKWGVDYTLGRRLYQMILDANFSHPQI
                     TFNQPVFAKGENKRLLELSVAEAGPSFVRAGLITEGELRTLLTEMRRLADDERVLALM
                     PRMSQVSARKLST"
     gene            complement(2366..4216)
                     /locus_tag="VFH91_01110"
     CDS             complement(2366..4216)
                     /locus_tag="VFH91_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6861604.1"
                     /translation="MNDTPKLCFGPYLLDEANECLRRDDEAIPLRPKAYGVLRYLLSH
                     PGVLVTKQQLLDAVWPDTFVGDSVLKDSIRQLREALGDEAKSPQFIETRHRRGYRFIA
                     PLREAPPRNATSNTSISSRFSAYVIPNDERVTSDMLGRDENLAQLHSWLKKALGGQRQ
                     TVFVTGEAGIGKTTLVEAFLKDLTDNMEILIGRGQCLEQYGSGEAYLPVLDAFSKLAR
                     DTLSEIVVEKLRLFAPSWLVQMPGLLTLRELDSFREQTTQTTRERMLREMTETIEAIS
                     TKRPFILFLDDLHWSDYSTVDLISYLARRSQPASFMLIGTYRPVDVALNEHPLKGTKQ
                     DLLMHKFCSDLPLEYLSEGSVATFLNNRFPSNSFPSRLAEMIHRHTEGNPLFMVNVVD
                     YLIDRELILESDGQHHLAVSPDEIELGVPENIRELIEDQVERLSDEEQRILEGASVVG
                     MDCSAVAIAAGLNSDVIKVEEICDGLARRQHFLLPAYLAELPDGTITPRYRFIHATYL
                     DVLYGRVAPTRRSQIHGRIGERGEAIYGDMVGVIAAELAVHFEEARDLARAVKYLELA
                     AENAESRSANHEALSLARRGLDLISTMPISAEGAIHKEKFANRISALEGI"
     gene            4251..4523
                     /locus_tag="VFH91_01115"
     CDS             4251..4523
                     /locus_tag="VFH91_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861605.1"
                     /translation="MTDLTLALILRRVTDVWQHSGSFVIRFSRATDPCTRRVSGRAEH
                     VATGKMIRFASMEELVKFMTRVLEEVRLNFEQADTLIEEILPPQDS"
BASE COUNT         1286 a         1345 c         1347 g         1247 t
ORIGIN      
        1 ttagtgctaa cggcaagacg ttgaccgtaa gcagcgtggg aatgaatgcg accgcacaaa
       61 atgggggcat tgaatacgcc aatggaccac aagctcgcac gatcttcagt ttccagatcg
      121 acggtctcaa tcagtcgatc gcgttcagtc cattggccaa caagactttt ggggctgctc
      181 cgttttcagt ttcggctacc gcaagttcag gtctaccagt gtcattcgcg gcaagcggca
      241 actgcacggt ggcgggagcg agtgtgacgt tgacgggggc cggaagctgt acgatcaccg
      301 cgtcacaggc tggtgattca aacttcagcc cagccttgga cgtctcacag actttctcga
      361 tcgccaaagc accggttacc gctacagcag gaagtggcct ggccatttac gatggattca
      421 caaagacacc ccctgcttgt acggtgacag gcgcgtacgt agggagtctg ggttgcgtca
      481 attctcctgt gtcagttggg ccaacgcccg gcacaacgca gatcagtccg attgtaagcg
      541 gcgacacctt gagcaacttt gacgtaacgc ccgtcaacgg atcgttcacg atcggctcgt
      601 tgaaggtttc gaccgatggc attcgaactg agctcaatga tgctcttgcg accgcgacga
      661 atcgcaggga cattacgaga cttaaagatg ccatctcgag cctcgaacag gcggttagtc
      721 caggtcgatg gacagctgac ggtggccagc ttgtgtgcaa cggtggctca aaagtgttcg
      781 gtagcgatca ggatgcaatc cttcagctga tggcgatgat tcgggatacg acgcccggta
      841 tccccgatgc aactatccaa cgctggatta acatactggt ggcgatcgac cgaaacctgg
      901 cccaaaccgc catcaccgct gcaagtagtt caggcgtttc tgctaagaag attgctgacg
      961 ctctggatga gttggctaac ggtgacgccg acgctgctcg cggtgattac gacaaagcga
     1021 tcggccacta tcagacggct tggaacagag tgacgagttg taactaggcc aagtcaactc
     1081 ggcattgaag gagtgatccg aagatccaga cgctacggtt ggcgacgcgg tccgacgagg
     1141 acgttgcgtc gctaaccgtg gcgtaccttc gacgtacaat ttgatgcagt aatcttcatc
     1201 gttctcgcca gattcaacct caaaactgct gtttgtattc ggtaattact gcccctcttg
     1261 ataatctgcg gtcgctgcat cagtaaaagt gacctcgacg acccacacaa aaacacgtct
     1321 tcaacattac aacttcctca tgactcatgt gctcgcgagg tctagtctcc agatccaata
     1381 gagagctagc ggcttgatcg gaaaaagcat cgctaagttc ttaatgacaa tcatttgaag
     1441 gaaggatctg tggaagatgt tagacatgaa ttgggttcag gaccaaacca atgggaatgg
     1501 tgtgaaaatc aacccctcgt catccaaggg ggactacgtg ttggcgaccg gtagcgccgc
     1561 tgcaaacagg ttacgtattc tgcacaacat ttatggcccc ggcgctcgta gtctgttggt
     1621 ccggtctggt ctcaggcaag gaatgagagt tgcggacctc ggttgcggcg tgggaatggt
     1681 tacgcaaatg ctcgctgaac ttgtcggccc taccggcgaa gttatcggag tagactttag
     1741 taaggcccag gttgaacagg caaaggcctt gctgccggca ggaatcacaa acgttgaatt
     1801 catcgaagct agcgcaaccg ataccggact tgccagagaa tcatttgatt ttgtgtattg
     1861 cagattctta ttaatccatc tccccgatcc gagagccgct ttgcgggaga tgtaccagtt
     1921 gttgaaacca aacggccggt tggtttgtga agatggtgac ctgacctctg ccggcagcga
     1981 gccaccgtct cagctccagg catttgcaac actatttgga aacctgggcc caaaatgggg
     2041 agtcgactac acgctcgggc gtcggctata tcaaatgatt cttgacgcta atttttccca
     2101 cccgcaaata acctttaatc aaccagtttt cgccaaaggt gaaaacaagc gattgttgga
     2161 actttcagtt gcagaagctg ggccgtcatt cgttagggca ggcctgatta ccgaagggga
     2221 actaagaacc ttgctaactg aaatgcggcg gctggctgac gacgaaagag tccttgcgtt
     2281 aatgccgcgg atgagtcagg tatcagcgcg aaaattatcg acgtaacccg gttctaataa
     2341 gtgagttact tatggattcc cgggattaga tcccttcgag cgcagagatt ctattcgcaa
     2401 atttctcttt gtgaatcgct ccctctgcgc ttatgggcat tgtcgagatc agatcaagtc
     2461 cgcggcgggc gagtgataga gcttcgtggt tggcagaacg gctttccgca ttctcagccg
     2521 ctaattccag atacttaaca gccctcgcaa gatcgcgagc ttcttcaaaa tgaaccgcca
     2581 actccgctgc tattactcca accatatctc cgtagatcgc ttccccacgt tcgccgattc
     2641 gcccgtgaat ctgcgaacgt cttgtaggtg ccacccttcc atacaacaca tccaagtacg
     2701 tcgcatggat aaaccgatac ctgggcgtta tcgtgccatc tggcaattca gctaaatatg
     2761 ccggcaatag aaaatgttgg cgtctcgcaa gcccgtcaca aatctcttca acttttatta
     2821 catcactatt caatccggcc gcgatggcaa cggcggagca atccatacct actacacttg
     2881 caccctccag tatgcgttgc tcttcatcgc tcaaacgttc gacctgatcc tctattaact
     2941 cccgaatgtt ctcgggtaca cctaactcaa tctcatccgg cgagactgcc aggtgatgct
     3001 gaccgtcgct ttcgagaatt aactccctat ctatcaggta atccaccacg tttaccatga
     3061 agagcggatt gccttcggtg tgtcgatgga tcatttcagc aagtcgggaa gggaaagaat
     3121 ttgatggaaa tctgttgttc aggaaagtcg cgactgaccc ctcactcaag tactccaatg
     3181 gcaaatcgga acaaaactta tgcatcaaca gatcttgttt ggttcctttc aaagggtgtt
     3241 cattcaaggc tacgtcaacc ggacgataag taccgatcaa catgaagcta gccggttggc
     3301 tccggcgtgc aaggtaagag attagatcga cggttgagta gtcgctccaa tggaggtcgt
     3361 cgagaaataa gataaatggc cttttagtcg agatggcttc tatggtctcc gtcatttctc
     3421 gaagcatccg ctcacgagta gtttgggtcg tttgctcacg gaagctatcg agctctctta
     3481 aagttaaaag gcccggcatt tgaactaacc aggagggtgc gaatagtcga agcttttcaa
     3541 caacgatttc gctgagtgtg tcgcgggcga gtttcgagaa tgcatcgagg acaggaaggt
     3601 atgcttctcc ggacccatat tgttccagac attggcctct accaatcaag atttccatgt
     3661 tgtccgtaag gtccttcaag aaagcctcaa cgagtgtggt ctttccgatc ccggcttctc
     3721 cggttacgaa aaccgtttgc ctctgacccc ctaaagcttt ctttagccag ctatgcagct
     3781 gggccagatt ctcatcgcgg cccagcatgt cactggtaac acgctcgtca ttgggaatga
     3841 cataggcgct gaatcgtgaa ctgatgctag tgttggaggt agcgttcctc gggggcgcct
     3901 ctcgcaatgg ggcgatgaag cggtatccac gccggtggcg ggtctcgatg aactgtggtg
     3961 acttagcttc gtcacccagc gcctcgcgca attgcctgat actgtctttc aaaactgagt
     4021 cgccgacaaa ggtgtctggc caaacagcgt cgagcaattg ttgcttggta actagaacac
     4081 cagggtgtga aagcaaatag cgaagtacgc cgtaggcttt tggacgaaga ggaatcgcct
     4141 cgtcgtcacg acgcaaacac tcgtttgctt catcgagaag gtagggacca aaacaaagtt
     4201 taggcgtgtc attcattaca acaaaacgac cgcaacaaga ctaattgtcc atgactgatc
     4261 tgacccttgc ccttattctc cgtcgcgtga cagacgtatg gcaacactca ggatcgtttg
     4321 tgattcgatt ttctcgagct accgatccgt gtacacgccg ggtttcagga cgagctgaac
     4381 atgtggcgac aggcaaaatg attcgctttg catcgatgga agagctggtc aagtttatga
     4441 ctagagtgtt ggaagaagtc cgtctgaact tcgaacaggc cgacactctg atcgaagaga
     4501 ttctaccacc gcaggacagt tgagaagctc tgcctcataa agccgaaagt ccgacctctc
     4561 attcacaccg ggcttcagcc cggtgatgaa cggaacaaga gacgcggaac cgttttaacg
     4621 gtttgctctt actggtcaat gcactgaggc agttgaatac gaggagcccg gtgtgagtga
     4681 gaggtcggac tttcagactt ttgaggcaaa gcccagttga gaagctccat cggtcatcat
     4741 cagtgctgga cccgagctga aagcgaaaag gcgcgacagt ttttggaacc gtcgcgccta
     4801 atctaaatgt ggtcaacgtc ctcaccccgc cgaccactgt gatggaagga ttcaccaacc
     4861 atctagttga gtcggactat acgctatacc tatctcagcc gcaaatcgac agcaacgtcc
     4921 gagggttgaa ccgaatcttc tatgatggtt ttcttaggaa ttactcgcca cagaggcata
     4981 gatcttagga acttagcgga gatcgtagtc gatctcgcgc gcggctttac ttaacttctg
     5041 caaacatcct taattaatga ttgtgttctc cgtgaaacag tctccagcta gcgccgaatg
     5101 caacgaaggg gtgaggtcct gtgattccaa gaactctcgt gccgatactc gcatcgattg
     5161 caaggtttcg cccaatcgta tacgcgacgc caccatcgag aatcgcagcc gactggcccc
     5221 ggctt
//
LOCUS       DASSFP010000054         4748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1349917, whole genome shotgun sequence.
ACCESSION   DASSFP010000054 DASSFP010000000
VERSION     DASSFP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349917"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFH91_01120"
     CDS             <1..448
                     /locus_tag="VFH91_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6861606.1"
                     /translation="GSPGSFAAVSLVATRNWPTNTEVKLVIVNDEWSVPIVEDLIPAV
                     GEAIEEENRGEMAWAEKIAAQAESIFQGSTLRVSRLIREGDPKSELPKIAEEWGADCI
                     FVGSTGFSNRIERFVLGSVSAAVAARAHCSVEVVRSLSVDKSSPQI"
     gene            492..1394
                     /locus_tag="VFH91_01125"
     CDS             492..1394
                     /locus_tag="VFH91_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6861607.1"
                     /translation="MTSETASETFPALEFRNVSLNFNARPVLTDISFQLAHGEMILVL
                     GESGSGKSVLLRLAIGLSRPDSGQIFVRGREIETLSEDKLLAIRGGLMGMVFQEESLF
                     TGLNVFENAAYRPREHGWKDEEVARAVTEVLTFVGLEGEEEKFPEQLSGGMKRRLEIA
                     RALIGWPSIMLFDEPTMSLDPMVAVQVLDLVMRARDINHISSMYVTKKPAEIPYLSGF
                     SASIKEDGKIVVEEAPADRLPPVRIMALEDGKISFIGSAPEFANCDLPSVQRMLTLEH
                     HDHSGDPYFADPWDKTRHPTDKLL"
     gene            1545..3746
                     /locus_tag="VFH91_01130"
     CDS             1545..3746
                     /locus_tag="VFH91_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6861608.1"
                     /translation="MLGKTISHYRITSQLGAGGMGVVYEAEDTNLGRHVALKFLTPAM
                     AGDDSLLQRFQREARAASALNHPNICTIHGIEQFESEHFIVMELLEGESLADRIRRGP
                     LDIDSVLTFGIQIVDALESAHSKGIVHRDLKPANMFITSRGQAKILDFGLAKIDHRQK
                     PDASSMLATVRKEELTSAGSTIGTVAYMSPEQARGQITDARTDIFSFGTVLYQMATGV
                     LPFQGETSAVVFESILNRDPTPVNQINPGLPAELNRIITQALEKDRDLRYQSATDLKT
                     ALKRLKRDLDSGQRTAEQSSGIRGRAAATDHSLAVLYFENLSGMKEDEYLRDGITEDI
                     TTELSKIKGLKTFSRAMVLAYRDKSVTAGQVGKELGASYVLVGSLRRAGARLRINAQL
                     VDAATDFPAWSERYDREMEDVFEVQDEIAQKIASALRITLSPQEQQALSAKPTENLQA
                     YDLYLRGRNYVRRVARQDLQFALQMYENAVALDPEFALAHAGLANVCAQYYYHFERQQ
                     QWIDRAVAATQKASATGHDSPEIQLAEAWVIFAEGRHGEAIEKLRMALSRTPDLDGGY
                     YLLGRALFEAGRYQEVVDMMEDALAHAGENYNTAIPIHNALGALGKKDALQNYLHREI
                     AIYEAHLKKVPEDARARVLLAGTYAQQGRFDEAKREADMAIVLRPDDSMVLYNTACTF
                     CAMNNVEDALNALRKAWEAGYRDPTWTRQDPDLALLHGNPEFERLYPAAGK"
     gene            complement(4064..>4748)
                     /locus_tag="VFH91_01135"
     CDS             complement(4064..>4748)
                     /locus_tag="VFH91_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861609.1"
                     /translation="EAQFPATVWGDFLQRLTTDLLRDARHIRKHHLQGLGSQSYGSLV
                     RKCVKGSTTVALLGTGNLVEEILPWLNEKDIAVRLFYRSWVHARRLMDRYPAVSLSRF
                     EMEDALWMRKSSALIVAAPIRSGEITRWIELQETRFSIVVDLRGEAAEDQIDCGIPVV
                     KLTELFDSLRSEKERLAQLTSKALKEIELLSRRRAVLTAPGRPLESRLPGTRVQSFAR
                     FDQQKLVVH"
BASE COUNT         1153 a         1185 c         1284 g         1126 t
ORIGIN      
        1 cggttcgcca ggaagtttcg ccgcggttag cctggtggct accagaaatt ggcccaccaa
       61 tacagaagtg aaactcgtaa tcgttaacga tgagtggtcc gttccgatag ttgaagatct
      121 cattccagct gttggcgaag cgatagaaga agaaaacaga ggtgaaatgg catgggccga
      181 aaagatagcg gcccaggctg agagtatttt tcagggttca acgctgcgag tgagccggtt
      241 gattcgagaa ggagatccga aaagcgaatt accaaagata gccgaggaat ggggcgccga
      301 ttgtatcttc gtaggatcga ctggttttag taaccgcatc gagagattcg tgctcggtag
      361 tgtgtctgct gcagtcgcag cgcgagcgca ttgctcggtc gaagttgtgc gttcattatc
      421 ggttgataaa agcagcccgc agatataact tcgctctgga tgaatcacgt tctctttcat
      481 tggagcgata tatgacatcg gaaaccgcgt ctgagacatt ccctgctctt gaatttcgaa
      541 atgtgagttt aaatttcaat gccaggcccg tgctgacaga tatctcgttt caacttgctc
      601 acggcgaaat gatccttgtc cttggcgagt cgggatcggg aaaatcagtt ttgcttcgcc
      661 ttgcgatcgg actctcgcga cccgattcag gccagatctt cgtgcgcggc agggaaatcg
      721 aaacactgtc cgaagataaa ctccttgcta ttcgaggcgg cttgatgggc atggtgtttc
      781 aagaggagtc gttgtttacc ggtctgaatg tctttgagaa tgcagcatat cgcccgagag
      841 aacatgggtg gaaagacgag gaggttgcca gagcggttac ggaagtcctt acctttgtag
      901 gtttagaagg agaagaggaa aagtttcccg aacagttatc aggcggtatg aagcgtcgtc
      961 tcgagattgc gagagcctta attggatggc cctcgatcat gttgtttgat gagccgacaa
     1021 tgagtcttga tccaatggtt gctgtccagg tgcttgattt ggtcatgcgg gccagggaca
     1081 tcaatcacat atcttcaatg tacgttacca agaagcccgc agaaattcct tatctgtcgg
     1141 ggttttctgc ttctattaaa gaggacggaa aaatcgtagt tgaggaagcg cctgccgacc
     1201 ggttgccacc ggtgcgaatc atggcgttgg aagacggcaa gataagcttt atcgggtcgg
     1261 cgcctgagtt tgcaaattgt gatttgccca gcgtgcagag aatgctgact ctggagcatc
     1321 atgatcattc cggtgatccc tactttgcag atccctggga caagacccga catccaaccg
     1381 ataaactact ctagcccatt catgtagctg agccggtaca gcttttatga gtaaccagat
     1441 ttttggattt tgcgtgagtt ttgtgcgcgg gccgggctgt actactatga ccggtagagc
     1501 gtgctttcat cgttatctca gaaacgtcgc ctggaggaac gcccatgctt ggcaagacca
     1561 tctcccacta tcgcatcact tcgcaacttg gcgcgggtgg gatgggagtt gtttatgaag
     1621 ctgaagacac gaacctgggt cgtcatgtcg ctctcaaatt cttaactcct gcgatggccg
     1681 gagacgacag tctgctacag cgcttccagc gagaagcacg tgccgcctcg gccctcaatc
     1741 atccgaacat ctgtaccatt cacggcatcg aacaatttga gtcagagcat ttcatcgtca
     1801 tggaattgct cgagggcgag tcgctagccg atcgaatccg acgtggacca ctcgacatcg
     1861 actctgtgtt gacgttcgga atacagatcg tcgacgcgct cgagtctgct cattcaaaag
     1921 ggattgtgca tcgtgatctg aagcccgcga atatgttcat aacctcgcga ggacaggcaa
     1981 agattctgga ctttggattg gctaaaatcg atcaccgcca gaaaccagat gcctcctcaa
     2041 tgttagcaac agttcgtaag gaagagctga cgtctgctgg ctcgacaatc ggtacagtcg
     2101 cctacatgtc tccggaacag gcgcgcggac agatcacgga cgcgcgcacg gacatttttt
     2161 ctttcggcac tgtcctctat cagatggcaa ccggggttct tccattccag ggtgagacgt
     2221 ctgcagtagt tttcgaatcg atcctcaatc gagatccaac gccagttaac cagatcaatc
     2281 cagggctgcc cgccgaactc aaccgcatta ttactcaggc tctcgagaaa gatcgtgacc
     2341 tgcgctatca gagcgcgaca gatctgaaga cagcgctgaa gcgtttgaag cgcgatctgg
     2401 attcaggaca gcgtactgca gagcaatcga gtggaatacg aggccgagca gcagctacgg
     2461 accattcgct cgccgttctt tatttcgaaa atctcagcgg catgaaagag gacgagtatc
     2521 ttcgcgatgg aattaccgaa gatatcacca ctgagttgtc gaagattaag ggattgaaga
     2581 cattttcgcg cgcgatggtt ttggcttacc gggataaatc cgtaacagct ggtcaggtcg
     2641 gaaaggaact cggagcatct tatgtgctcg ttggcagttt gcgtcgagcc ggcgcgcgac
     2701 tccggatcaa tgctcagctc gtcgatgccg caacagattt tccagcttgg tccgagagat
     2761 acgatcgcga aatggaagat gtgttcgaag tgcaggacga gatcgcacag aagatagcct
     2821 cagctttgcg tatcacgctc tcgccgcagg agcagcaagc gctgtcggca aaaccgaccg
     2881 agaatttgca ggcgtacgat ctctacctca gaggtagaaa ctatgtgcgt cgggtcgctc
     2941 gacaggatct ccaatttgct cttcagatgt atgagaacgc tgtcgcactc gatcctgaat
     3001 tcgcgcttgc tcatgcggga ctggcaaacg tttgtgcaca gtactactac catttcgagc
     3061 gccaacagca gtggattgat cgagctgtgg cagcgacgca aaaagcgagc gccactggac
     3121 acgactcgcc tgaaattcag ctagccgaag cgtgggtgat ttttgccgag ggtcgtcacg
     3181 gagaggcaat tgaaaagctc aggatggcac tcagccggac gccggatttg gatggtggtt
     3241 attacctgct ggggcgcgcg cttttcgaag ccggacggta tcaggaagtg gtggacatga
     3301 tggaggatgc gttggcccat gcaggcgaga actacaacac tgcgattcca attcacaatg
     3361 cgctcggcgc acttggaaag aaagacgctc ttcagaatta cctccatcgc gagatcgcta
     3421 tatacgaagc acatttgaaa aaggtacctg aggacgctcg tgctcgcgtc ttgttggccg
     3481 gtacatacgc gcagcaagga cggttcgacg aagcgaaacg ggaggcagac atggcgatag
     3541 ttctgcggcc cgacgattcg atggtgctct acaacaccgc ctgcactttt tgcgcgatga
     3601 acaacgtcga ggatgctttg aatgcactca ggaaggcatg ggaggctgga tatcgggatc
     3661 ctacatggac cagacaagac ccggaccttg cgctccttca tgggaatccg gaattcgaac
     3721 ggctctaccc ggcggccggg aaataaagtt gcctcctgaa gggtcgacgg tgtcgcgatc
     3781 ggtgaatcgt tcagctcccg cctggaaaaa atgaacctct cccacaagga taaattccac
     3841 caacggtcgg agactgtgtc aaaactcgga ttaccgcctg tttcttgttc gtgttatttc
     3901 gtgtgatgcg cgtggatcgc ctgttacgct ccgagaaaag cgatccacga aataacacga
     3961 aataacacca acgaagttcc atggcaaatt agaatttcga cacagtctct gccgttggtg
     4021 gaatttcggg aagcgcggat cagggtcgct tgtaggttcg actttaatgg accaccaact
     4081 tctgttggtc gaatctcgca aagctttgga cgcgagtccc tggtagccgg gactctaaag
     4141 gacgacctgg tgccgttaaa actgcacgtc gtcgcgataa caattcaatc tctttcagcg
     4201 cttttgaagt cagctgggcc agcctctcct tctcgctcct taacgagtcg aagagttcgg
     4261 ttagtttgac tacaggtatc ccacaatcaa tctgatcctc cgccgcttca ccgcggagat
     4321 cgacaactat cgaaaacctc gtttcctgta actcgatcca tcttgttatc tccccggatc
     4381 gtattggggc ggcgacaatg agagcggacg acttcctcat ccataaggca tcttccattt
     4441 caaagcgact aagactgacg gctggatatc ggtccattag ccttcgcgcg tgaacccaac
     4501 tccgataaaa cagacgcacc gctatatcct tttcattcaa ccagggaagt atttcttcga
     4561 ccagatttcc cgttcccaat agcgcgactg tggtactacc tttcacacac tttctgacta
     4621 aactgccgta cgactggctc ccgagaccct ggaggtgatg ttttcgaata tgtctcgcat
     4681 cgcggagcag atcagttgtc aatctttgca gaaaatcacc ccagactgtg gcggggaact
     4741 gcgcttcg
//
LOCUS       DASSFP010000055         4437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1204350, whole genome shotgun sequence.
ACCESSION   DASSFP010000055 DASSFP010000000
VERSION     DASSFP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204350"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFH91_01140"
     CDS             complement(<1..271)
                     /locus_tag="VFH91_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861610.1"
                     /translation="MTTLVSKREASPALVDDSDAIVAAQGQGVYKIRHTLASRFAFLV
                     ICVAIVSTALAYGTVHYWALGLFNLGAVAILILWVVDSWGLRTLRV"
     gene            complement(240..1544)
                     /locus_tag="VFH91_01145"
     CDS             complement(240..1544)
                     /locus_tag="VFH91_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861611.1"
                     /translation="MPQELFQINSQKTLIRVLMLVLLLVALFWSLFAIRWYLGNTLAE
                     YFNPSENSIDVAQFASSLAPNDPLPHWRLGQVYQQKLPVDQIGQTIAEYQKAISLSPN
                     DYRFWLALGTAQEQAGDVADGEIALRRAVALAPAYSFPHWYLGNLLLRSSRYDEAFTE
                     LRIASEADNELRPQLFNFVSAVYGRDLESIKRALGNDGAVRVQFCLYLINQKSFDDGL
                     AMWSSIDADSKQANRSTGDEIVKALIGASRFHNAVTVYNDLAPTDAYRTEIGKVLDGG
                     FEESMAHGANSVFGWRVGAVSQMQLGIDPAKSHSGRRSLRLLFQVKTKLQDINVSQQI
                     AVLPNTEYDFECFVSTEKLESGSTPLIEIVDASDQTVLGTSSPVATGTNDWSRIAFTF
                     KTKPKSEGVIIRVVRSPCEGDVPLCPIFGVMWYDDFSFKKRS"
     gene            complement(1560..3356)
                     /locus_tag="VFH91_01150"
     CDS             complement(1560..3356)
                     /locus_tag="VFH91_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861612.1"
                     /translation="MPFSPRTLLAVAASFGLALVLTPLVRAFARRFGVVAKPKIDRWH
                     KEPTAMLGGVAIWLSVVVSILVFMPPTTYGWVILRATSLLFLVGLVDDLIHIKPYQKL
                     IGQILGSAFIVYYGLTLPWTGSVLLNMGLTIFWLIGITNAINLLDNMDGLASGISIIA
                     AGFLALGFVNTGQYMEGLMLLIFAGALLGFLVFNSNPASIFMGDCGSMFAGFFLASSA
                     LINVSGGRSRSFLPVLAVPILVLFIPIFDTTLVTVLRKLSGRAASQGGRDHTSHRLVA
                     LGMSERHAVWMLYGFAALSGLLAILVQRARLDVSLAAIAAFTILLTLVGVYLAGVKVY
                     DQTEEEIAFKDKPLYAFLVDVSYKRRIFEVLLDVILIILSYWCAYAINFPSGSPAWNL
                     FIRTLPVIVFMKMPVFLAMGVYRGLWKYTSIGDLIVFAKAVFMSSIASVVMVLFTFRF
                     EGFSRTVFAIDAVFLFLFLAGSRLAFRLFRQILPAVHAKDGRRVLIYGAGDGGELLLR
                     EIRNNRDLNLSPVGFLDDDPAKTGKVIHGLKVFGANGDLGHVCEQQDIHEVVISSVKM
                     DLERVQEVVRSCSERQITVKRMRITLEDLTEN"
     gene            complement(3585..>4437)
                     /locus_tag="VFH91_01155"
     CDS             complement(3585..>4437)
                     /locus_tag="VFH91_01155"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET6861613.1"
                     /translation="HLKGHPNFHYTIESVHNKPVTAELVDQSDVIFHLAAAVGVKLIV
                     ESPVRTIETNVRGTEVILALANKKKKKVLVASTSEVYGLSSDVPFREDGNLVMGATTK
                     GRWSYACSKAIDEFLALAYWREKKLPTIVVRLFNTVGPRQTGQYGMVIPTFVKQALAG
                     RPITVYGDGQQSRCFGYVGDVVGALVKLMDHEQAVGQVFNIGSNHEISILKLAERVKE
                     LTDSTSEIVFVPYDEAYEEGFEDMPRRIPDISKVSGLVGFNPEMSLDGILESVIDYQS
                     GRRSAAG"
BASE COUNT         1227 a         1179 c         1031 g         1000 t
ORIGIN      
        1 tcacgcgcaa tgtccgcaac ccccatgaat caaccaccca caatatgagt atggcgaccg
       61 cgcccagatt aaacagtccc aacgcccagt agtgaaccgt tccataagcg agtgcggtgc
      121 taacaatcgc tacacagatc accaggaaag cgaatcgact tgccaatgtg tggcggattt
      181 tatagacgcc ttgcccttga gccgcaacta tagcgtccga atcatcgacc aacgccgggc
      241 tagcttctct ttttgaaact aaagtcgtca taccacataa caccgaatat aggacacaac
      301 ggtacgtcgc cttcgcaggg agaacgcacg actctgataa tcacaccttc agatttaggt
      361 tttgtcttaa aggtaaaagc gatgcgactc cagtcattcg taccagtggc aaccggagac
      421 gaagttccca gaacagtttg atccgaagca tcaacgattt ctatcagggg cgttgagccg
      481 ctctcgagct tctctgtact cacgaaacac tcgaagtcgt attcggtatt cggaagcacg
      541 gctatttgct gtgaaacatt tatgtcttgc agtttggttt taacctgaaa cagtagcctc
      601 aagctgcgac gtcccgaatg actttttgcc ggatcgatcc ccaactgcat ctgcgaaact
      661 gctcccacac gccaaccgaa gactgagtta gcgccatggg ccatactttc ttcaaagcct
      721 ccatcaagta cttttccgat ctcagtgcga taggcatcgg ttggtgcaag atcgttatag
      781 accgtaaccg cattatgaaa tcgcgaagcc ccaatgagtg ctttgacaat ctcatctccg
      841 gtggatcgat tggcctgttt tgagtcggcg tcgatactag accacatagc caatccatcg
      901 tcgaaacttt tctggttgat caggtagaga caaaactgaa cccgtacagc tccgtcgttt
      961 cctaatgccc gcttgataga ttccaaatca cgtccataaa cagctgagac aaagttaaaa
     1021 agttgcggcc ttaactcgtt gtcggcctcg ctcgcaatcc gtagttcggt aaatgcttcg
     1081 tcgtagcgac tgcttcgcaa taacaagttg cccaggtacc agtgcggaaa tgaatacgcc
     1141 ggcgcaagag ccacggctcg tcgcaaagca atttcaccgt cggcaacgtc tcccgcttgt
     1201 tcctgagccg ttccgagcgc aagccagaac cggtaatcgt ttggagacaa actgattgcc
     1261 ttctgatact cagcgatggt ttgcccgatt tgatccaccg gcaatttttg ttgatagacc
     1321 tggcccaacc tccaatgtgg caaaggatca tttggcgcta acgaactcgc aaactgtgca
     1381 acgtctatac tgttctccga agggttgaag tattcagcaa gcgtattacc caaataccag
     1441 cgaatggcga agagcgacca aaaaagcgca actagcaaca gaaccagcat taacactctg
     1501 atcagggttt tttgcgagtt gatttggaac aattcttgcg gcatatcaat tccagcgcgt
     1561 cagttttcgg ttaaatcctc cagagtgatt cgcatacgct taactgtgat ctgtcgttca
     1621 ctgcaagaac gcacgacttc ctggactcgt tcaagatcca tctttacgct cgaaataact
     1681 acttcgtgga tatcctgttg ttcgcaaaca tgacccagat caccgttggc gccgaagacc
     1741 tttaagccat gaataacctt gccggtcttt gcgggatcat cgtcgagaaa cccgaccggc
     1801 gacaagttga gatcacgatt gtttcgaatc tctcgtaaca gaagctctcc cccatccccg
     1861 gcaccgtaga taagcactcg acgaccatct tttgcatgta cagcaggcag aatctggcga
     1921 aatagtctga acgcgaggcg gctgcccgcc agaaaaagaa aaaggaatac ggcatcgatt
     1981 gcgaaaacag ttcgggagaa tccttcaaaa cggaatgtga aaaggaccat taccacactg
     2041 gcgatcgagc tcataaacac cgctttggca aaaacaatca gatcgccgat gctcgtatat
     2101 ttccagagcc cacgatagac ccccatagca aggaaaactg gcatcttcat gaacacgatc
     2161 acgggaagag ttcgaatgaa gagattccag gccggacttc cgctggggaa attaatagcg
     2221 taagcacacc agtaggagag gataatcaga ataacatcca gcaggacctc aaagatgcgc
     2281 cgcttgtaag agacgtcaac cagaaatgca tagagcggct tatccttgaa ggcaatctct
     2341 tcttcggtct ggtcataaac tttgacacca gccagataga cgccgactaa cgtaagcaga
     2401 atcgtgaagg ctgcaatcgc cgcaaggctg acatcgagcc gggcacgttg gaccaggatt
     2461 gcaagtagac ccgagagggc agcaaacccg taaagcatcc acaccgcatg tctttccgac
     2521 attcccaaag cgactaaacg gtgcgacgta tgatcgcgtc ccccctgaga tgcagctctt
     2581 cccgaaagct ttcgcaagac cgtaaccagc gtggtgtcga agattgggat gaacaggacg
     2641 agaatcggga ccgcaagtac gggcaggaaa cttcgagatc gtccaccact aacgttaatg
     2701 agcgccgaac tcgcaagaaa aaaacctgcg aacatcgacc cgcagtcgcc catgaagatt
     2761 gaagcgggat ttgaattgaa cacgaggaat cccaacaatg ctccggcaaa gatcaagagc
     2821 atcaggcctt ccatgtactg gccggtattc acaaaaccaa gcgcaagaaa acctgcagca
     2881 ataatggaga ttccagacgc caaaccatcc atgttgtcga gcaggttgat cgcgttggtg
     2941 ataccgatca gccagaagat ggtcaacccc atattcaata gcacagaccc ggtccaaggc
     3001 agggttagtc cgtaatagac aataaacgca gagccaagga tctgaccgat caatttttga
     3061 tacggcttga tgtgaattag gtcgtcgacc aggcccacta gaaacaacag actggtggcg
     3121 cgaagaatca cccaaccgta cgtggttggg ggcataaaca ccaggatgct cacaaccact
     3181 gataaccaga tagccacgcc gcccaacatt gctgttggtt ctttatgcca gcgatcaatt
     3241 ttgggtttag cgacaacacc gaaacggcga gcaaacgccc ttacaagtgg agtaaggacc
     3301 agggcaagac caaaagatgc ggcgactgcc agcagcgtgc gcgggctaaa aggcatattc
     3361 aatcgaggtc aggggataat agaatgttcg cctggaggca ggttgacttg cagcactttg
     3421 caacaacgtc atttttgaaa gccgcttcac aggcctattt tcgtaggaaa actattcgat
     3481 atgaggcggc aaagcaagcg gacatcaaaa agattgaaca attgtgaggt ttggtcagcg
     3541 cagtactgcc caccgccacc acgtgactga agcctgttgt tatttcatcc tgcagcagaa
     3601 cgacgtccgc tttgataatc gataacactt tcaagaattc cgtccaggct catttcgggg
     3661 ttaaagccta ctaagccact caccttggaa atgtcgggaa tgcgtctcgg catgtcttca
     3721 aaaccttctt cgtaggcctc gtcgtaaggg acgaagacta tttcagatgt cgaatccgtt
     3781 aactctttta ctcgctctgc cagtttgaga atcgaaattt catggttcga tccaatgtta
     3841 aagacctgac caaccgcctg ttcatggtcc atcaatttaa ccaaagcccc aacaacatcg
     3901 cccacgtagc caaagcaccg gctctgttga ccatcaccgt aaactgtaat cggtctgccg
     3961 gcgagtgctt gcttaacgaa cgtcgggatt accattccgt attgtcccgt ctgccttggt
     4021 ccaaccgtat taaagagtct caccacaatc gtcggcagct ttttctcgcg ccagtacgcg
     4081 agtgccagaa actcatcaat cgccttgcta cacgcatagc tccatcgccc tttagttgta
     4141 gcgcccatca cgaggttccc atcttcgcgg aagggaacat ccgaactcag accataaacc
     4201 tcggaagttg aggccacgag gaccttcttc ttcttcttgt tcgcaagcgc caaaatgact
     4261 tcggtccctc gcacattggt ctcaatagtt ctcactggac tttcaacgat tagtttgact
     4321 ccaactgcag cggcaaggtg aaagatcacg tctgactggt cgactaactc agcggtgact
     4381 ggtttgttgt gaacactttc aatcgtgtaa tgaaagtttg gatgaccttt aagatgg
//
LOCUS       DASSFP010000056         5157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1949005, whole genome shotgun sequence.
ACCESSION   DASSFP010000056 DASSFP010000000
VERSION     DASSFP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949005"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFH91_01160"
     CDS             <1..766
                     /locus_tag="VFH91_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6861614.1"
                     /translation="KVGNRTNIVTRWQALEYITSQNMDRRAFASLNCITVVPGAVGAW
                     RKDLLLEAGGFSSDTLAEDQDSTLRIRRLGYKIGYEETAVAWTEAPDDLRSLARQRFR
                     WAYGTLQCMWKHLDALFRPRYGTLGFIAMPNVWIFQILFPLISPIMDLMLVYTLVSAA
                     LDRLQQPTGYSFANLQQVLIYYALFLAIDWLSACFAFVLEKRERWRLLWWLFLQRFCY
                     RQVMYYVMIKSVAMAIRGPVVGWGKLERKATAEARP"
     gene            858..2540
                     /locus_tag="VFH91_01165"
     CDS             858..2540
                     /locus_tag="VFH91_01165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6861615.1"
                     /translation="MRLRQCIGFNPGENETTHELSTDIDKRHGRRVWIAYLSLFLVAL
                     GFRLFVALRLPNDTPGDGVIYAQIARNVLEQHVYSHETQAPFIPSLIRLPGYPLFLAG
                     IYTVFGHGNNTAVRVLQALLDTITAGLIALVAYLWISDDVLKHRAAIAAFVLAAICPF
                     TTIYVATILTEVPTNLLVVLLCLFVTLALKSQSKRWRERLSWAIAGFTVGVAVLFRPD
                     SGLLGAAAGITILLASVGASDQRRLWSQRFSHSVVSAAIFSVAFCLVLVPWTIRNRRV
                     FGLFQPLAPMHAEMPGEFVPRGYLLWVRSWLDDARFVGPVLWDLDNKQIKVETIPAYA
                     FDSPEEKERVADLFAKYNKTEAQAPASTSNPAPPAAPSSTPDEEESSDSEESDDDPDE
                     SADEQPDESDVEMTPDIDAGFAQIAKERISRHPVRYYLLLPARRAVALWFDTHSQYWP
                     FDGDLLPLKDLDYDIHQQWWLPSFAGLTWLYTILGAIGGWFMWRSRIFQMRLLLSFAA
                     LIIFLRLGFFSTMENPEPRYTVEFFPLLSILGGIALAHLLRRRQSLEPVAES"
     gene            complement(2537..3214)
                     /locus_tag="VFH91_01170"
     CDS             complement(2537..3214)
                     /locus_tag="VFH91_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6861616.1"
                     /translation="MKKIIFVLAFISLNATLSFGQNTNSSTTTSARPRTSSSPAPKTA
                     TQKSTDIEQPKTTTTVPKTEAKKPVVSAQAAPGSDGVLAAFNGLLNGIRHASVAEVTN
                     AYWNSPRLSLFNYNGTVTKGWEQVKKNRESSYPEIKDVKLDVRDVAVTMLGRDAALVS
                     CQWTQTQTYKGTPETASGRMTLVFRRVGTAWKAIHLHSSPDTPNPASVPPSEQIEKPS
                     PTPTPTP"
     gene            complement(3288..4781)
                     /gene="gltX"
                     /locus_tag="VFH91_01175"
     CDS             complement(3288..4781)
                     /gene="gltX"
                     /locus_tag="VFH91_01175"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6861617.1"
                     /translation="MAVRVRFAPSPTGYLHIGAARSALFNWLYARKVGGKFLLRIEDT
                     DLQRSTEESTRSIIEGLEWLGLDYDEDLVFQSANAPLHRAAALRLVEEGKAYRDFTPK
                     EQRDDKNVKEAIAERARAHAAEGIDQRSNPYRDLSREESDRRAAAGEPFAVRLKVAVE
                     GKTNFHDIVYGPQERSYSEIEDLVLLRSGGHPLYNLSVVIDDIEMGITHVIRGQDHLT
                     NTHKQVLIYEALGAEVPEFAHLPLILAPNKGKLSKRKHGEAVSLTTYRDRGFVPAAFR
                     NFLALLGWSPPDGKEILSKAELVQSFSLEGIGRANAVFNFQENDPRHWTDDKALWMNA
                     EYIRTMALDELIPMVKSELRSNKLWREEYDEDDREWLLKTVDLIRQRFFTLKDFSEQG
                     RAYFSDDFDFDDAAVNKNLRKEPRLRELLNGLAAKLESLKAFTDETAESALREFAEEA
                     GVKAGLLINGSRTMLTGQSVGPSMFEIFDVLGRDRSVERLRSGIPWN"
     gene            complement(4899..4989)
                     /locus_tag="VFH91_01180"
     tRNA            complement(4899..4989)
                     /locus_tag="VFH91_01180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4953..4955),aa:Ser,seq:gct)
BASE COUNT         1133 a         1291 c         1322 g         1411 t
ORIGIN      
        1 aaaagttggc aatcgcacca acatcgttac tcgctggcaa gctcttgagt atataacctc
       61 gcaaaacatg gatcgtcgcg cgtttgcgag tcttaattgc attaccgtag tgcctggcgc
      121 cgttggagcg tggaggaaag atttattgct cgaggcgggt ggtttctcgt cagacactct
      181 tgccgaagat caggactcaa ccctgcgaat tcggcgactg ggctacaaga ttggttacga
      241 agagacagcg gtggcctgga ccgaggctcc cgacgatttg cgttcgttgg cgcgacaacg
      301 ttttcgctgg gcatacggaa ctctccagtg tatgtggaag cacctcgacg cccttttccg
      361 gccgcgatac ggaacgctcg gtttcattgc aatgccaaac gtctggattt tccagatcct
      421 ctttcctctg atttcgccga tcatggatct gatgctggtc tacacgttgg tgtcggcagc
      481 tcttgatcgt ttgcaacaac caactggtta ttcgtttgcg aatcttcagc aggtcctcat
      541 ttattacgcg cttttcctgg cgatcgactg gttgtcagct tgcttcgcat tcgttctcga
      601 gaaacgagag cgatggcgac tgctctggtg gttattcttg cagagatttt gttatcgaca
      661 ggttatgtac tatgtaatga tcaaatcggt agcgatggcg atccgtggcc cagttgtcgg
      721 ctggggaaaa ctggaacgca aggcgacagc tgaagctcgc ccgtagcgca ttgagtcttt
      781 gtgcggaact cgttcaccat tgagtgcgag gacgaggcaa cctttgaagt ttaaaaccgt
      841 tccaatcggc gtccgctttg aggctgcgcc aatgcatcgg gttcaatccc ggtgagaatg
      901 agactacgca cgagttgtcc acagatatcg ataagcgaca tggtcgcagg gtttggatcg
      961 cctacctttc gttgtttctg gttgctctcg gctttcgact ttttgttgct ctgcgccttc
     1021 ctaacgacac acctggcgat ggagttatat acgctcagat agctcgtaat gtacttgagc
     1081 aacacgttta ttctcacgaa actcaagctc cgtttattcc ttcccttatc agacttccag
     1141 gctacccgtt gtttctcgcg ggcatataca cggtttttgg tcatggtaac aacactgcgg
     1201 tgcgtgtgtt gcaggcacta ctggacacaa ttacagctgg cttgatagcg ctggtagctt
     1261 acctttggat atctgacgac gttctaaaac atcgagctgc aatagctgcg ttcgtgcttg
     1321 cggcgatttg tccgtttaca acgatttatg ttgccaccat cctcactgag gtacctacca
     1381 acctgctcgt agttctgctt tgcctatttg taactcttgc gttgaagtct cagtcgaaga
     1441 gatggcgaga acgtttgagc tgggccatcg ccggctttac ggttggcgta gcggtactat
     1501 ttcgtcccga tagcggactc ttaggcgccg ctgcgggtat cacgatcttg cttgcgtcgg
     1561 taggtgcttc cgatcaacga cgactgtggt cacaacgatt ctcacattca gtcgtttcag
     1621 ccgcgatttt ttcagttgcg ttttgtttag tactggttcc ctggaccatc cgcaatcgaa
     1681 gagtcttcgg attatttcaa cctctcgcgc cgatgcacgc tgagatgcct ggagagttcg
     1741 tgcctcgagg gtatttgctg tgggtgcgat cgtggcttga cgacgcacga tttgtcggac
     1801 ctgttctctg ggatctcgat aacaaacaga tcaaggtcga gacgattccg gcttatgcgt
     1861 ttgattcacc ggaagaaaag gagcgcgttg ccgacctttt cgcaaaatac aacaagaccg
     1921 aggctcaagc acctgcgtca acgtctaatc cagctccgcc agctgctcca tcttccacac
     1981 ccgatgaaga agagagctct gattcagaag aaagtgacga tgacccggat gagagcgctg
     2041 atgaacaacc ggatgagagc gatgttgaga tgactccgga catcgacgct gggtttgctc
     2101 agatcgcaaa ggagagaatc tcgagacatc ccgtacgcta ttacctgctc ttgcctgcca
     2161 ggcgcgcagt tgccctttgg tttgatacgc actcgcaata ctggccgttt gatggagatt
     2221 tactgccact caaagacctc gattacgaca ttcaccaaca gtggtggctg ccatcttttg
     2281 cagggcttac gtggctctat acgattctcg gagcaatcgg cggatggttc atgtggcggt
     2341 cgcgaatttt tcaaatgcga ctgctattga gttttgcagc tctaataatc ttcctgcgcc
     2401 tgggattctt ttcgacaatg gagaatccag agccaagata tacagtcgag ttttttccac
     2461 tcctatcgat tcttggcggc atcgcccttg cgcatttgtt gagacggcga caaagcctcg
     2521 aaccggtagc agaatcctaa ggcgtcggtg ttggggtcgg tgatggtttc tcgatctgtt
     2581 ctgatggagg aacgctggcc gggtttggtg tgtctggtga tgagtgtaaa tgaatagctt
     2641 tccacgccgt accgacgcgc ctgaatacga gtgtcattct accggatgca gtttcaggag
     2701 ttcctttgta ggtttgcgtt tgagtccact ggcaactgac aagcgcagca tctcgcccca
     2761 acatcgtgac ggccacatcg cggacatcaa gcttgacgtc cttgatctcg ggatatgagc
     2821 tctcgcgatt cttcttaact tgttcccagc ctttggtaac agttccgttg tagttgaata
     2881 gactgagtcg tggcgagttc caataagcat tggttacttc tgcgacactc gcatgtcgaa
     2941 ttccgtttaa taggccgtta aaagcagcca ggacaccgtc ggagccggga gctgcttgcg
     3001 cactcaccac cggttttttt gcttcggttt ttggtacggt agtggtggtt ttgggctgct
     3061 caatatcagt tgatttttgc gtcgcagtct ttggtgcggg gctgctactc gttcgtggcc
     3121 gcgctgaggt ggttgtagat gaatttgtgt tttgaccaaa agagagggtt gcattcaacg
     3181 agataaaggc aagtacaaaa ataattttct tcatcgagtt ttactcctga ctggaatttt
     3241 tgcgtctatt tggtaatcgt cttgcttcct gtcgggtaat tcttcactta gttccaggga
     3301 atgccgctgc ggaggcgctc aactgatcgg tcccgcccga gcacatcaaa aatttcaaac
     3361 attgaaggtc caacggattg tcccgtcagc attgtgcgcg atccattaat gagtaggccc
     3421 gccttaacac ctgcttcttc ggcgaattct cttagagcac tttcggcggt ttcgtcagtg
     3481 aaggctttca atgactctaa tttggcggcg agaccgttaa gcagttctcg aagccgcggt
     3541 tccttgcgca ggtttttatt gacagccgcg tcatcaaaat caaaatcgtc cgagaaatag
     3601 gcgcgacctt gttctgaaaa atccttcaaa gtaaaaaagc gctgcctgat taggtcgacc
     3661 gttttcaata accactcccg atcatcttcg tcgtattctt cgcgccacag tttatttgat
     3721 cgcaattcac ttttgaccat cggaatgagc tcgtctaaag ccatcgtacg aatgtactca
     3781 gcattcatcc acagagcctt gtcgtcagtc cagtggcgtg gatcattttc ctgaaaatta
     3841 aaaactgcat tagcccggcc aataccttcg agcgaaaaac tttggaccag ctccgctttg
     3901 cttaaaatct ctttaccgtc gggtggagac cagcccagta acgccaaaaa atttcgaaat
     3961 gcagccggta caaagccacg atctcggtaa gtagttagcg atacggcttc tccgtgtttt
     4021 cgtttcgata actttccctt attgggcgcc aggatcaacg gcaagtgtgc gaactcagga
     4081 acttcggcgc caagtgcctc ataaatcagt acctgcttgt gcgtattagt cagatggtcc
     4141 tgcccccgaa tgacatgagt gatgcccatt tcgatgtcat cgatgacaac agataagttg
     4201 tagagaggat gccctcctga cctaagcagc accagatctt ctatttcaga ataacttcgc
     4261 tcctgtggac cataaacgat gtcatgaaaa tttgtttttc cttcgactgc gactttcaag
     4321 cgcactgcaa aaggttcgcc tgctgctgcc cggcggtcag actcttctct cgaaagatct
     4381 cgatagggat tgcttcgttg atcgatacct tcggcggcgt gagcacgagc acgctcggca
     4441 atagcctctt tcacattttt gtcgtcacgt tgctctttag gagtgaagtc gcgataggct
     4501 ttgccctctt caaccagacg aagcgcggct gcgcggtgga gtggagcgtt ggctgactga
     4561 aagactaaat cctcatcgta atccaacccg agccattcaa gaccttcaat gatcgagcgc
     4621 gttgattctt cggttgagcg ctgcagatcg gtatcttcga ttcgtagtaa aaattttccg
     4681 cccactttcc gtgcatagag ccagttgaag agagcggatc gagccgcacc gatgtgcagg
     4741 tatcctgttg gtgatggagc aaaacgtact cgaaccgcca tagtttaatg ttgacgataa
     4801 gacggcgcgg gtgcaagaca aaaattaacg agtgacaaag aaccggaatt aacaagcttt
     4861 gaagcctgtt tcgtcgtttg tcactcgttc atattaactg gcggagatga caggattcga
     4921 acctgtgaag ggcttttgac ccttaacggt ttagcaaacc gccgccttca gccactcggc
     4981 cacatctccg tgccggaatc ggcgaagacc aattctagtc ggcatccgcg tattgtcaag
     5041 gtaaggtaga atcgccgcgt gcctgaattt tcgccgagtc ggcgcggttt cctcaacggg
     5101 ttagtcgcgg cacccctgct ggctgtcgct gcgacctcgg cttacgcgag attgatt
//
LOCUS       DASSFP010000057         6876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_459848, whole genome shotgun sequence.
ACCESSION   DASSFP010000057 DASSFP010000000
VERSION     DASSFP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459848"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VFH91_01185"
     CDS             complement(<1..1152)
                     /locus_tag="VFH91_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HET6861618.1"
                     /translation="MRLITALFLTSCFAVSLQPNIKSQDQGVELRTGQTIERSLKSGE
                     THDFTVSLQENWLLQLAVEQRGIDVVIRISSPEGRTIREIDTPTGDEGSENVSFVATT
                     AGQYRVSVYPLVSSATVSASGRYELKVLDCRAATSDEVNLGKNIEANTAKGKALLKEL
                     IEKVAQLKASRNKIGFQLRIASMLESSDTKLAKEMLDAAVEAFADFVSTLNEDDEDNY
                     LPQYSTAVQLRDELIQALTARDPDKALEFMKTSKLLLHVDKEVSEGDDKQESVYILNI
                     ANQISKSDPKRTLQLAQDVLKKQYSPVLTSTLQSLKETSPNLAEQLETQIVEKLTNET
                     IIKNPDAAQLTNALLREQAIAGGVVKADGRVKLFEKLLAECLAYSPKRGI"
     gene            complement(1264..2358)
                     /locus_tag="VFH91_01190"
     CDS             complement(1264..2358)
                     /locus_tag="VFH91_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6861619.1"
                     /translation="MPAKTPAKRTARMTPAVSNKRRAAIEKRQRAATLITGGTGFLGS
                     HLVRQLVEDGARDLRVMATSIPDWLVDLGVETFSGSITDPEDVKRAVEGITEVYHLAG
                     KVSRERKDAREMYEVHVEGTRLLCDAAKASGVKTIVLASTSGTIAVSEDGEVIPDETY
                     AAPLEIISRWPYYASKTYQEMAALERFTGRGLRLVIMNPSLLLGPGDDRLSSTKVVLD
                     FMAKKIGAVPTGGISFVDARDAALTFRNAMKKGRHGERYLLGAANWTFNKFFGRLERL
                     TKISAPRFALPARIAVGGAQLVDSFFKQWDWASPVEPGAIEMAQYYWYLNCSKAAREL
                     AFKPRDPSETLHDTVSYLRENFLGSNAFGK"
     gene            complement(2376..6734)
                     /locus_tag="VFH91_01195"
     CDS             complement(2376..6734)
                     /locus_tag="VFH91_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6861620.1"
                     /translation="MIGSTGFLGKVTLSMLLYRFPNIGKVYITVRARSQEESETRFWN
                     NIITAPPFDPLRERYGSAFEGFVRDKVIIRGGDIGETNFGYTEEEAQAIADDVDVVIN
                     SAGNVTFNPTLESGLRTNVVGTQNVVAFTRRMKRPALVHVSTCFVAGNRSGAVWESDP
                     VLGYFPRKHELQGVEFSVDKEIADCAKLAERVRDEARDAMMAARFRELARKRLNEEGR
                     DVDDPDALGLAVARERKVWTRTRLTDLGVERAGYWGWPNIYTYTKSLGEQLVAAETNM
                     VRSIVRPSIVESALAYPFPGWNEGFTTTAPIIFLIRKGQTAIPANDKLILDITPVDQV
                     ASVMLAVAAQACIEEPRLVFQAATGDSNPNNMERIIGLVGLDKRRHFKEKETGVKVLN
                     EIASRIEPRPVSTSYYERTSAPMLNSAAKKTSSLLDRVRPRWGGGRVAEVIDRVKDSV
                     ERVEELTRETSDAFDMFRPFTVENAYVFRSDNVRSLFDRISPDERSLLTWNPEQFDWY
                     HYWLNVHLPGLAKWVFPTLEEDMRAQPKRVYTYRDLLELFETSTKRHATRVAMRIERD
                     GRKEQYTYADLRELATRAAGFLASQGIKHGDRVMLVSNNTPEWGMSYFGVLKAGATCI
                     PCDPESSTAEILNFAKAGNAAGIIISQTVRTDHPDLEEQLLQQGATRTWTYEELFELP
                     DEQVEDERIALLPHKVHAQNVASLIFTSGTTGQPKGVMLSHRNLTSMVSMLSSVFDMT
                     TRDGVLSVLPLHHTFEFSTGFLTPLSRGAQITYMPELTSDALARAIKNGHVTGMVGVP
                     ALWELLHRRIKNRLYEHSDLIGKTAENIIRANAWLRDKTPLNLGQVLFYPIHEGLGGR
                     IRYFISGGSALSEKIQRDFHGLGFTILEGYGLTEASPVLTVTRPENKMITGTVGKALP
                     GIEVKIAEPNINGVGEVIARGPNVMLGYYEDETSTRQTLVDRWLYTGDLGRVDDDGNL
                     YLVGRSKEIIVDTNGKNVYPDELEELYSNSSYIKELSIVGLPDGIGEKVACIVVPDDE
                     YDISLSRAELHRLIEEHFREVSASLPYFKRVKVLHFTDMELPRTATRKVKRREVVQII
                     ESIEQTQKSKRKAGGRETADGDSQWVLGVVASVSNRSRSEVTVDSRLGDLGFDSLMFV
                     ELATAIENAGGSITSPERFNEIQDVRELLTVVSKQAAATRTERIKADEQTSDDEIYIP
                     SLVRSAGTKAGDMLQRLFYDQFLRTHYVGRDNIPPHTNFIVAANHCSHLDMGLTKMAL
                     GEYGSDLVALAAADYFFDTKYKRAVMENFTNLVPMERTGSLRQSLRHARSFLDRGYNA
                     LIFPEGTRSMTGVMADFKPVIGYLALHARVGILPMFLDGTYEAMPKGTTFIKSREVGA
                     RIGRFLPIEELEEMTKGMARAEAYRLIAARVRHEVENLRDGTRYRFDAKALRKQWKAE
                     RRITTTEEENEVVSVG"
BASE COUNT         1564 a         1803 c         1702 g         1807 t
ORIGIN      
        1 aatccccctt tttggcgagt aagccagaca ctctgctagt agtttctcaa ataactttac
       61 tctgccatca gctttaacga caccacctgc tatggcttgc tcgcgtaata gagcattcgt
      121 caactgggca gcgtcgggat tcttgatgat ggtttcgtta gttagctttt cgacaatttg
      181 agtttccagc tgctcagcaa gattcggaga tgtctctttc aaggactgaa gagtactggt
      241 caaaactggt gaatattgct tcttcaagac atcctgagct agctggaggg tgcgtttcgg
      301 atctgacttt gaaatctgat ttgcgatatt gagtatgtag acgctctctt gtttatcatc
      361 gccttcacta acttccttgt ctacgtgcag caacagcttg ctggttttca tgaactccaa
      421 cgctttgtct gggtctcgag ctgtgagggc ctggatgagt tcgtctcgaa gttgtaccgc
      481 ggtcgaatat tggggaaggt aattatcctc gtcgtcctca ttaagagtgg aaacaaaatc
      541 ggcgaatgcc tcaactgctg catccagcat ttccttagcc aactttgtgt cgctggactc
      601 gagcatcgac gcaatcctta attgaaatcc aatcttgttt cgcgaagctt tgagttgcgc
      661 caccttttcg atcaattctt tgaggagtgc ctttcctttt gcagtatttg cttcaatatt
      721 cttacctaag ttcacttcgt cggacgttgc cgctcgacaa tccaggacct taagctcgta
      781 acgtccgctg gccgataccg tcgctgaact tactaacggg tacacgctca ctctatattg
      841 accggctgtg gttgccacga agcttacgtt ctcactgccc tcatcgccgg taggcgtgtc
      901 gatttcacga atcgttcgcc cctcaggtga gcttatgcga attacgacat cgatgccgcg
      961 ctgctcaacc gccagttgca ggagccagtt ctcctgtagt gagactgtga agtcatgggt
     1021 ctcaccggat ttgagggacc gttcgattgt ctgaccagtc cgtaattcga caccctggtc
     1081 ctgactctta atgtttggtt gcagtgatac cgcgaagcaa gaggttagga ataatgcggt
     1141 aataaggcgc atctgaccct ccatttgtta tgaccggctt taacacacac gtttactgcg
     1201 gaatgagagg aaaacggtaa cttagacgct gtaaagagcc gttaagttcc ccggatgaag
     1261 gatttacttt ccgaatgcgt tgctgccgag gaaattctcg cgcaggtagg agacggtgtc
     1321 gtgcaatgtt tcgctgggat ctcgaggttt gaaggccaat tcccgcgccg cttttgagca
     1381 attcaggtac caataatact gcgccatttc gatggcaccg ggttcaactg gtgaggccca
     1441 atcccattgt ttgaaaaaag aatcaaccag ttgagcaccg cctacagcaa tgcgagctgg
     1501 caacgcgaaa cgaggcgctg agattttggt gagccgttcg agacggccaa agaacttatt
     1561 gaaggtccaa tttgccgcac ctagtaaata acgttcaccg tgacgtccct tcttcatcgc
     1621 attccgaaac gtcaacgcag catctctggc atccacaaaa ctaatgcccc cggttggcac
     1681 tgcgccaatc ttcttagcca tgaagtcgag cacgacttta gtcgaactca aacgatcgtc
     1741 tcccgggcca agtagcagac ttggattcat gatgacgagg cgtagacctc tgcctgtgaa
     1801 gcgttcaagt gcagccatct cctggtaggt cttactcgca tagtatggcc agcgtgagat
     1861 tatctcgagg ggagctgcgt aagtctcatc cggaattacc tcgccatcct cactgacggc
     1921 aatcgtcccg ctggtcgaag ccaggacaat tgttttaacg ccggatgctt tggctgcgtc
     1981 gcacagtaag cgcgttccct cgacgtgaac ttcatacatt tcacgagcgt ctttacgttc
     2041 acgcgagact ttgccagcaa gatggtaaac ttcagtgatt ccctcgacag cacgtttgac
     2101 atcttccgga tctgtaatgg aacccgagaa tgtctccacg ccaagatcga ccagccagtc
     2161 aggtattgag gtcgccatga ctctcaagtc tcgagcaccg tcttcgacga gctggcgcac
     2221 gaggtgcgat cccaggaagc ctgttccacc cgtaataagc gtggcggcac gctgccgctt
     2281 ctcgatggca gcgcgtcgct tgttactaac tgctggagtc atccgggcag tgcgtttggc
     2341 gggagtcttt gcgggcatat taaactcggc ctaaatcaac caaccgaaac tacctcgttc
     2401 tcctcttctg tagtggtgat tctccgttcc gccttccact gctttcgtag agccttggcg
     2461 tcgaacctgt aacgagtgcc atcgcgaagg ttttcaacct cgtgtcgcac acgggcggcg
     2521 atcagccgat aagcctctgc tcgggccatt cctttagtca tttcctcgag ctcttcaatc
     2581 ggcaggaagc gtccaatgcg cgcgccaact tctctgctct taatgaaggt tgtgcctttg
     2641 ggcattgcct cataagttcc atccagaaac atgggaagaa taccgacgcg tgcatgaagt
     2701 gccagatatc caattactgg cttgaaatcc gccatcacgc cggtcataga tcgagttcct
     2761 tcgggaaata tgagcgcgtt atagcctcga tcaaggaatg atcgtgcgtg tcgaagcgat
     2821 tgtcgcaacg aacctgttct ttccatcggt actagattcg taaagttctc catcactgca
     2881 cgcttgtatt tcgtatcgaa gaaatagtct gctgcagcca gggccaccag atcgctgccg
     2941 tattcgccaa gagccatttt ggtcaggccc atatccaggt gcgagcaatg atttgcagcc
     3001 acgatgaagt ttgtatgggg tggaatattg tctcgaccca cgtagtgcgt gcgtaaaaac
     3061 tgatcgtaaa agagtcgctg tagcatgtcg ccggcctttg tgccggccga cctgaccaat
     3121 gagggaatgt aaatctcatc atcagatgtc tgctcatcgg ctttaattct ttcggttcga
     3181 gttgctgctg cctgcttgct tacaactgtt agcagttctc gaacatcctg aatttcattg
     3241 aatcgttccg gagatgtgat tgaaccgccg gcattctcaa tagctgtagc aagctcaaca
     3301 aacatgagac tatcaaaccc gaggtcgccc aaccttgagt caactgtgac ttccgatcgc
     3361 gaccggttgg acaccgatgc gacaacgccc aacacccatt gggaatctcc gtcggctgtc
     3421 tcacgaccac ctgctttgcg tttactcttt tgagtctgtt cgatcgactc gatgatttgg
     3481 accacttctc gacgtttcac cttccgcgtt gccgtacgcg gcaattccat gtcggtaaaa
     3541 tgaagcactt tgacgcgttt gaaatatggc agggaggctg agacttcgcg gaagtgttcc
     3601 tcaataagac gatgaagctc agcgcgtgag agtgagatgt cgtattcatc atccggaacg
     3661 acgatgcatg caactttttc cccaatccca tccggcaggc ctacaatgct cagctctttg
     3721 atgtacgaag agtttgaata cagctcttcc agttcatcgg gataaacatt cttgccgttg
     3781 gtatcgacaa tgatctcttt agaacgacca accagataca gattcccgtc gtcatcgact
     3841 cggcccaggt cgccggtgta gagccatcta tccacaagcg tttgacgtgt cgaagtttca
     3901 tcttcgtaat agccgagcat tacatttgga ccacgagcga taacctcacc aacaccgtta
     3961 atgttcggtt cggcgatctt cacttcgatt cccggaagag ctttgccgac cgtgccggta
     4021 atcatcttat tttccggcct ggtgactgtt aataccggcg acgcttctgt tagtccgtat
     4081 ccttcaagaa tcgtgaagcc gagcccatga aaatcacgtt gaatcttttc actcaacgcc
     4141 gaaccgccac tgatgaagta acgaatccgg ccgccaagtc cttcgtggat tggatagaag
     4201 agaacctgac ccaagttcag cggagtctta tcgcgcagcc aggcattcgc ccgaatgata
     4261 ttctctgccg tcttcccaat taagtcgcta tgctcgtaga gacgattttt gattctacga
     4321 tggagcaatt cccacagcgc cggcaccccc accattccag ttacatggcc attcttgatt
     4381 gcccgagcca gcgcatcgct agttaactcg ggcatatagg tgatctgcgc tccgcggctt
     4441 agcggagtca gaaagccagt cgagaattca aacgtgtggt gaagcggtaa cacagataaa
     4501 acgccgtcgc gtgtcgtcat gtcaaagaca gacgagagca tcgaaaccat gctcgtcagg
     4561 ttacgatgcg agagcattac gccttttggc tgaccggtgg tgcccgaagt aaagattagg
     4621 gacgcaacgt tttgcgcatg aactttgtgg ggcagaaggg ctattcgctc gtcttccact
     4681 tgttcgtcag gtagctcgaa cagttcctca taggtccatg tgcgagtcgc gccttgttgc
     4741 agcagttgtt cctcgagatc tggatgatca gtacgaactg tttggctgat aattatgccg
     4801 gcagcatttc cagccttggc gaaattgaga atctcggcag tcgagctttc aggatcgcaa
     4861 ggtatacagg tcgctccagc tttgagtacg ccgaaataac tcatacccca ctcgggagtg
     4921 ttattgctga cgagcatgac tcgatcaccg tgcttaatac cttgactggc caggaagccg
     4981 gcggctcgag tagctagctc gcgtaaatcg gcatacgtgt actgctcttt tctgccatct
     5041 cgttcgatac gcatggcaac gcgagttgca tgtcgcttgg ttgaggtttc aaagagttcg
     5101 agcagatccc ggtacgtata tacgcgtttg ggttgagcgc gcatatcttc ttcgagtgtt
     5161 ggaaagaccc acttcgccag gcccggaaga tgaacgttca gccagtagtg gtaccaatcg
     5221 aactgctcag gattccaggt gagtaacgag cgttcgtcgg gcgaaatgcg atcgaagagt
     5281 gatctgacat tatccgaccg aaaaacataa gcgttttcga ctgtgaacgg acgaaacatg
     5341 tcgaatgcat cgcttgtttc gcgtgtcagt tcttcaactc tttcaacgct gtctttaaca
     5401 cgatcaatta cctcggcaac gcggcctcca ccccatctcg gtctgacccg atccaatagc
     5461 gacgaggttt ttttggcagc agaattgagc ataggtgcgg acgtcctttc ataataggac
     5521 gtgctcactg gcctgggctc aatgcgcgag gcgatctcgt ttaggacttt tacgcctgtt
     5581 tctttttctt taaagtgtcg tctcttatcc agtcctacga gcccgatgat ccgttccatg
     5641 ttgttagggt tcgaatctcc ggtagcagcc tgaaaaacca gacgtggctc ttcgatacaa
     5701 gcttgagcgg cgaccgcaag cattacagaa gcgacctggt ccaccggcgt gatgtcgagt
     5761 atgagtttgt cattggccgg aatcgctgtt tgacctttgc ggatcagaaa gataattggc
     5821 gctgtggttg taaaaccttc gttccaacca ggaaacggat aagctaaggc cgattcgaca
     5881 atgcttggtc ggacaatact gcgaaccatg ttcgtctctg cggctacgag ttgttcacct
     5941 aacgacttgg tgtaggtgta aatgttgggc cagccccagt atccggcacg ctctacgcca
     6001 agatctgtca aacgcgttct ggtccaaact tttctttctc gggcaactgc gagtcccagt
     6061 gcgtccggat cgtcgacatc tcgtccctct tcgttcaaac gcttgcgcgc aagttcacgg
     6121 aagcgggcag ccatcattgc gtcgcgagct tcatctctta ctcgctccgc cagttttgcg
     6181 cagtcggcaa tctctttatc aacagagaac tcgactccct gaagctcatg tttccgaggg
     6241 aagtatccaa gcactggatc gctttcccat acggctccgg atcggtttcc ggcaacaaaa
     6301 caggtcgaaa cgtggaccag tgccgggcgc ttcattcgcc gtgtaaaagc aaccacattt
     6361 tgagtaccga cgacgtttgt tctaagtcca ctttcaagag tgggattgaa cgtaacgttg
     6421 cccgctgagt tgataactac atcaacatcg tcagcgatgg cctgggcctc ttcttcggta
     6481 tagccgaagt ttgtttctcc gatgtcgcca ccgcgaatta tgaccttgtc cctgacgaag
     6541 ccttcgaacg ccgatccgta acgttctctt agaggatcga aaggcggtgc ggtaatgatg
     6601 ttgttccaga accgtgtttc agattcctcc tgggaccgcg cccggacagt tatgtaaacc
     6661 tttccaatgt ttgggaagcg gtagagcaac atgctcagcg ttactttgcc gagaaagcca
     6721 gtgcttccaa tcaagaagat tttccgatcc tggtagattt ctgtcggtga taatttcata
     6781 actgataccg agtagtgcgt ttattccata tcagcaatgc cgatcatcgg ttgatgaagc
     6841 attgtgatct cggccgagtt acccatgtag cttcga
//
LOCUS       DASSFP010000058         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1500215, whole genome shotgun sequence.
ACCESSION   DASSFP010000058 DASSFP010000000
VERSION     DASSFP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500215"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /gene="serC"
                     /locus_tag="VFH91_01200"
     CDS             <1..550
                     /gene="serC"
                     /locus_tag="VFH91_01200"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET6861621.1"
                     /translation="SHAIPVEKYALIYAGAQKNMGPSGVTLVILRDDLLQRIPEGLHT
                     MLDYKIHVENKSLYNTPNTWGIYIIRLVCDWIKEKGGLGAMERENEQKAKLLYDAIDS
                     TGFYRGHAEPECRSIMNVTFRLPSEDLEKKFTTEATAQGLDGLKGHRSVGGIRASIYN
                     AFPLAGVEALVSFMKDFERRSG"
     gene            830..1810
                     /locus_tag="VFH91_01205"
     CDS             830..1810
                     /locus_tag="VFH91_01205"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6861622.1"
                     /translation="MGHDPNPVDLQTRTWKVDGDAVNKRLDAFLAAQIQGWSRARLQR
                     LIENEDILVNGKPAKASYKLRLNDDIDVELVSEPTSNFVPENIPLEIIFEDQTLLVVN
                     KPAGLVVHPAAGVHSGTLANALAYHFQNLPTQAHQLRPGIVHRLDRDTSGLMVVAKTD
                     EALERLSDQFRDRSVFKSYLALVHGNVNENSGRIDQPIARDPSNRTRMAVVRSGRSAL
                     SIYRVRQRYERFTLLDVELKTGRTHQIRVHLAWLKHPVIGDETYGGGRDNTIQDPSVR
                     TRVKNLGRHFLHAEKLSFDHPQTGARMEFTSQLPSELQIFLDFLGNKVLK"
     gene            complement(1812..>3768)
                     /locus_tag="VFH91_01210"
     CDS             complement(1812..>3768)
                     /locus_tag="VFH91_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6861623.1"
                     /translation="MSPEQAEGEEIDERTDIFSLGVVLYEMIAGEAPFKGRTDSHTRV
                     SVLDHDPTPLLHYDPQTPRQLERIVAKSLAKDKHKRYQTMTDLKLDLEQLRDELYSSR
                     TSRIDEPSEVRHPRTIETHAQSAATTLTREQVQTVTDQPDPLPEPHEPPARSKSMYLF
                     AGAILLAVLAALIYWAWSRQAPINSVAVLPFVNDSNDPNTEYLSDGITESIINSLSQL
                     PSLKVMSRNASFRFKDRGIDPQEAGNNLQVGAVLTGRVVKLEDQVVIRTELIKVSDGS
                     QIWGEQYSSNLANILSIQAEVSKKISQSLRLRLTGEDEERLAKRYTNDAEAYQLYLKG
                     RYFWNKRNEEGFRSGIEFFKRAEERDPTYALAFSGLADSYALLCDIGAARPVDEMPKA
                     KAAAQKAVDADSSLAEAFTSRAFVRLSYDWDWVGAENDFQQALKLNSKYPTAHQWYAS
                     YLMQMGKFNRAKGEIEQAYNLDPLSPIISSNAGLYSYYEHNYDDAITKYKLTLQSDPD
                     FWVARHYLALAYVQKGMYDEAISGLRDLIKAPRSGPISDQVIANETEASASLGFAYAM
                     AGKRAEALSILDQLRALSRRRYVSAIYLATVYAGLKDNDRAIEYLNAAFDSRHPGLVL
                     IRIEPMFDGLRADERFKKLAARFEPIP"
BASE COUNT          980 a          966 c          894 g          928 t
ORIGIN      
        1 gtcgcatgcg attcccgtcg aaaagtacgc attgatatat gcaggggccc aaaaaaatat
       61 gggaccaagc ggtgtaactc tcgtgatctt acgtgatgat cttttgcaaa gaattcctga
      121 aggtcttcac acgatgcttg attacaagat ccatgtcgaa aacaagtcgc tctataacac
      181 acctaacacg tggggtatct acatcatccg cctcgtgtgc gactggatta aggaaaaggg
      241 cgggcttgga gctatggaac gtgaaaatga gcaaaaagca aaactgcttt acgacgcaat
      301 tgactcaacc ggcttctatc gcggtcacgc tgaaccagag tgccgttcga taatgaatgt
      361 caccttccga ctaccgtctg aggatctgga aaagaaattc acgaccgaag caacggcaca
      421 ggggctcgac ggcttaaagg gccatcgatc agttggcgga atccgcgcct cgatctataa
      481 cgcgtttcct ctcgcgggag ttgaagcgct ggtatcgttc atgaaggact tcgaacgaag
      541 gagcggatag acgggaggtt aagggacagg gttatgagat cgttaatcat ttgagaattg
      601 tgagttgata tttgagaatt gttaagttgg actttgatct ttggcctttg acctttgatc
      661 tttggtagct tgccatggga acaaatcaaa ggccaaagat ccaagatcca agatcaaagg
      721 ccaaagccca aagcccaaag cccaagatca aaattaacaa ttctcaaata tcaattcata
      781 attctcaaat gatttaatcc ccgatagcct ttacctatcc accattctta tgggccatga
      841 tcccaatcct gttgaccttc aaactcgcac ctggaaagtg gatggcgacg ccgtcaataa
      901 acgactggac gcctttcttg ctgcacaaat tcaaggctgg agtcgagctc gtttacaacg
      961 tctgatcgaa aacgaagaca ttctggtaaa tggcaaaccc gcaaaagctt catacaaatt
     1021 acgattaaat gacgacatag acgtcgaact cgtttcagaa ccaacgagta atttcgttcc
     1081 ggaaaatatt ccactcgaaa taatcttcga agatcaaacg ctgctggtcg ttaataaacc
     1141 agctggacta gtggttcatc cggctgcagg agttcattct ggcacgttag caaatgcact
     1201 tgcctaccac ttccaaaacc tacccactca ggcgcatcaa cttagaccgg gaatcgtcca
     1261 tcggttagat cgtgatactt ccggactcat ggtcgtcgca aagaccgacg aagcgctcga
     1321 acgtctctct gaccaatttc gcgatcgttc agtcttcaaa tcctatctgg cccttgtgca
     1381 cggaaacgtt aatgagaatt ccggccgaat agaccagcca atagcacgcg acccatcgaa
     1441 tcgaaccaga atggccgttg tgcgcagcgg tcgctcggct ctttcaatct atcgtgtgcg
     1501 acaaaggtac gagagattta ccctgctgga tgtcgaactg aaaaccggcc gcacccatca
     1561 aattcgagtc catttggcgt ggctaaagca cccggtgatc ggcgacgaaa cctacggcgg
     1621 gggccgtgac aacaccattc aagacccgtc cgttcggacc agagtaaaaa acctgggccg
     1681 ccatttctta cacgccgaaa agttgagctt tgatcatccg cagacaggcg ctcgtatgga
     1741 gttcacctct caattgccat ccgaattgca gatctttttg gatttcctgg ggaataaggt
     1801 cttgaagtaa ttcagggtat gggctcgaag cgcgcggcca gtttcttgaa gcgttcatcg
     1861 gcacgtagac catcaaacat tggctcaatt cggatcaaaa ccagaccggg gtgccggcta
     1921 tcgaaagctg cgttcaagta ttcaatagca cgatcgttat ctttcagtcc cgcataaacc
     1981 gtcgcaaggt agattgccga aacataacgg cgtcgagaga gcgccctaag ttggtccagg
     2041 atagacaacg cttcagcacg tttgccggcc atcgcgtacg caaaacccag ggacgctgaa
     2101 gcttcagttt cgttcgcaat aacttgatct gaaatgggcc cggaacgagg cgccttgatc
     2161 aaatctctca agcctgaaat cgcctcgtcg tacataccct tctgaacgta agccaacgcg
     2221 agataatgcc gggcgaccca gaaatccgga tcagattgca acgtgagttt gtatttagtt
     2281 attgcgtcgt cgtagttgtg ctcgtaatag gaataaaggc cggcattcga gcttatgatt
     2341 ggtgaaagcg gatccagatt ataagcctgt tcaatctccc cttttgcacg attgaacttt
     2401 cccatctgca ttaaataaga cgcgtaccac tgatgcgccg tgggatattt tgaattcagt
     2461 ttcaatgctt gctgaaaatc attctctgct ccgacccaat cccaatcgta tgacaacctg
     2521 acgaacgcgc gcgaagtaaa ggcttcagcg agtgacgaat ctgcatcgac agctttttga
     2581 gcagccgcct tcgcttttgg catctcatcg acaggtctgg ccgcgccaat atcgcaaagc
     2641 aatgcgtacg agtcagccag accggagaag gctaaggcgt aagtaggatc acgttcttcg
     2701 gccctcttga aaaattcaat gccactgcga aacccttctt cgttacgttt gttccaaaaa
     2761 taacgaccct taagatatag ctgataagct tccgcatcgt tcgtgtagcg cttcgccagt
     2821 ctttcttcat cttcacccgt cagtcgtaaa cgcagactct gcgaaatctt cttcgatacc
     2881 tcagcttgta tcgacagaat attggccaga ttgctgctgt actgctcgcc ccatatttgc
     2941 gaaccatccg acactttgat caactctgtt cgaattacga cctggtcctc caacttcaca
     3001 acacgaccag ttagcaccgc gcctacctgt aagttattgc ccgcctcttg tggatctatc
     3061 ccacgatctt tgaatcggaa ggacgcattg cgtgacatta ctttaagact cggcaattga
     3121 gagagactgt tgatgatgct ctcagtgatc ccatccgata gatactcggt gttcggatca
     3181 ttactatcgt tgacgaaagg aagtacggcc actgaattga tcggtgcttg acgtgaccag
     3241 gcccaataaa tcagcgccgc cagcactgca agcaagatcg caccagcaaa caagtacatc
     3301 gatttacttc tggccggagg ttcgtgtggc tcaggcaaag gatcaggttg atccgtgaca
     3361 gtttgtacct gttcacgcgt caacgtcgtc gctgctgatt gcgcgtgagt ttcaattgta
     3421 cgcgggtgtc gaacctcaga tggctcatct attcgactgg tgcgtgagga atacagttcg
     3481 tcacgcagct gttccaggtc gagcttgagg tccgtcatcg tttggtagcg tttgtgctta
     3541 tcctttgcca gcgacttcgc cacaatgcgc tcgagttgtc gaggcgtttg cggatcataa
     3601 tgaagtaaag gagttgggtc gtggtccagg accgagactc tcgtgtgact gtcagtccga
     3661 cccttaaatg gcgcctcgcc cgcgatcatt tcgtatagga ccacacccaa actaaaaatg
     3721 tcggtcctct cgtcgatctc ttcgccttcg gcttgctcgg gcgacatg
//
LOCUS       DASSFP010000059         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_310057, whole genome shotgun sequence.
ACCESSION   DASSFP010000059 DASSFP010000000
VERSION     DASSFP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310057"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1887)
                     /locus_tag="VFH91_01215"
     CDS             complement(199..1887)
                     /locus_tag="VFH91_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HET6861624.1"
                     /translation="MTIRRCLLSVVPVLSLVFGCVLCASTQKVYSDESSVLQVTRPIS
                     VVFDPKSFGAKADGKALDSPAINKAIDAAAAAGGGTVWLTAGTYRAFSIHLRSNVALY
                     LDQGTTILAADPRDGDGKYDSPEPNQWNQYQDFGHSHWHNSLIWGENLENVSILGPGR
                     IWGKGLVRSGSQSRTKEQNDALGSAPPDPNGGPFGYPNPRDAVEPGWGNKSISLKLCR
                     NVIIRDVTIFHGGHFAILATGVDNLTIDNVKIDTNRDGIDVDACKNVRISNCTINSPF
                     DDGICPKSSFALGYSRVTENVTITNCQVSGYDEGTLLDGTYKRDYRAQNGTFSPTGRI
                     KFGTESNGGFKNITVTNCVFDYSRGLALEAVDGALLEDVTISNITMRDISNSPIFIRL
                     GARNRGPKETTRTGAIRRVVISNVVVFNADPRYSSIISGIPGFAIEDVTLQNIRIYSQ
                     GGGTKEQAGLEPPEKEDTYPEPTMFGDLPSYGFFVRHVKGLQLRDVQVSFLKSDLRPA
                     FWLNNVADAEFFQVKARSESNAPSFLLKNVSNFALQQSWPFPDQRLEKVELKKF"
BASE COUNT          639 a          617 c          570 g          618 t
ORIGIN      
        1 ccgcttctaa ggtttttaac ggggaatgaa ggatcttgct agttggagct taaacgggta
       61 ggaattctac tacgctgaac actgaaatcc cactgagcgc gagtttcgag caaagtaaat
      121 gatcgaatct ctagttcgtt cagccggaaa gtcctcgttc agcagctccc gttaaaacag
      181 ggagcaaagc gcaaaatttc aaaacttttt cagttcaacc ttttccaaac gctgatccgg
      241 aaacggccaa ctttgttgca atgcaaagtt cgacacgttc ttcaataaga aggatggagc
      301 gttactctca ctccgtgcct ttacctgaaa gaattctgca tcagcaacat tgttcaacca
      361 gaatgctgga cgtaagtccg actttaagaa actaacctgc acatcccgaa gttgcaatcc
      421 ctttacgtgt cgcacaaaga atccgtacga aggaagatca ccgaacatgg tcggttctgg
      481 ataggtgtcc tctttctcag gcggttcaag accagcctgc tctttggttc caccaccctg
      541 cgaatagatt cggatgttct gtaaagtcac atcttcgatt gcaaacccgg gaatcccact
      601 gattatcgat gaatacctgg gatcagcatt gaaaacgacg acattactaa ttacaacacg
      661 acggatcgcg ccggtgcgag tggtctcttt tggaccgcga tttctggcgc cgagacggat
      721 aaagatcggc gagttgctga tgtcgcgcat agtaatattt gagatcgtca catcttccaa
      781 cagagcgcca tccaccgcct caagcgcgag accgcgtgag tagtcgaaaa cacaatttgt
      841 taccgtgatg ttcttaaacc caccatttga ttcagtgcca aacttaatcc tacccgtagg
      901 actgaaagtt ccgttttgag cacggtagtc gcgtttatat gtaccatcca acaaagtgcc
      961 ctcatcataa ccactgacct gacaattcgt aatcgtcacg ttctcggtca ctcgcgagta
     1021 cccgagggca aacgagctct tcggacagat gccatcgtcg aatggtgagt taattgtaca
     1081 gtttgagatt cgcacattct tacaagcatc cacgtcgatg ccgtctcgat tagtatcgat
     1141 cttgacgttg tcgatggtca ggttatcaac accagtcgcg aggatcgcaa agtgtccgcc
     1201 atgaaagatc gtcacatcgc gaatgatgac gttgcggcag agcttcagac tgatcgactt
     1261 attgccccag cccggctcca cggcatcgcg cggatttggg tagccaaacg gacctccatt
     1321 aggatctggg ggtgcactac caagagcgtc gttctgttcc ttcgttcgcg attgactacc
     1381 actccgaacc agtccttttc cccaaatgcg gcctggtcca agaatagaaa cgttttcaag
     1441 attctcaccc cagatcagac tgttatgcca gtggctgtgt ccgaagtcct ggtattggtt
     1501 ccattggttt ggttccggtg agtcatactt gccatcgcca tcgcggggat cggcggccag
     1561 aattgtcgtt ccctgatcga gataaagagc tacgttactt cttaagtgaa tcgagaacgc
     1621 acgatacgta ccggctgtaa gccagactgt tccacctccg gcagcagcgg ctgcgtcgat
     1681 cgctttgttg attgccgggc tatcgagagc ttttccatcc gctttcgcac cgaacgattt
     1741 gggatcaaac actactgaga ttggacgagt gacttgaaga acactagatt catcggaata
     1801 aactttttgc gtactggcac ataacacgca accaaatacc aaggatagaa cgggaacaac
     1861 tgacagtaga catcttctaa tggtcattcg gtcctccgct gcgggaaata taaaatagct
     1921 agactccgcc gagatggtct taccattctt cagatacggc ttaaattgac acgacaaatt
     1981 tgggtgaaca gatcgcaact gtggacgtgt accacccttt tgcagaagat gtcaactctc
     2041 aagtttcact gagcttgcaa gtagatacgc taatttgttt attttgagaa caaaactctt
     2101 gacggcgaga atcgagcgtg atacaaggcc cttaaatagc cataatggtc ataccaatct
     2161 gtcttgtgta aagttaaatg gtcgcgcaat cgatgaagtg ttaaggggca gtcgatcttg
     2221 taagggagag cctatcgccc gttgctatgt ggactaactt tcattcgccg ggttagacct
     2281 cggcactttc aacaaccgcc agactcgccc agcgggagga gaagatcatg agattgagta
     2341 gcgtcgttaa aggccaccac atctgctctt gttgttcgcc cacttttttc accccagcaa
     2401 aatgcgcctc aatcccaggt cagaaggatc gtcagaagga tcct
//
LOCUS       DASSFP010000060         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1056490, whole genome shotgun sequence.
ACCESSION   DASSFP010000060 DASSFP010000000
VERSION     DASSFP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056490"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFH91_01220"
     CDS             <1..550
                     /locus_tag="VFH91_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861625.1"
                     /translation="PAPNAPPLEEGQRFEFKVVGPGSPAGGPQVMTAPLPPGALPGGE
                     NVTLSTAGAVGGATYVFRRKGTDANEVKEDLGEQLIEGVKAQGTRSTVTIPAGEIGND
                     RPIEIVSERWYSPELQTVVMTRHNDPRTGETTYKLTNVNRNEPAKSLFEVPADYTVKE
                     GLPGVPGVQVAPARVRKSNIPE"
     gene            complement(775..2883)
                     /gene="fusA"
                     /locus_tag="VFH91_01225"
     CDS             complement(775..2883)
                     /gene="fusA"
                     /locus_tag="VFH91_01225"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6861626.1"
                     /translation="MKVFATEAIRNLAVIGHGDAGKTQLISSLLFVAGATPRWGKVDE
                     GTTVTDHEEDSIARKITLNTALAHLEHRDTKLNLIDSPGYAAFIAHARPALRVADCAI
                     VVVDGVKGVEVQTEKTWAYANEFMVPRVMVITKLDKEHADFGMALQSAQNTFSRAIIP
                     FTLPIGKEKDFRGVVDVVHMKAYELGADGQAKEIDIPAEGRDLVDKTRERLVELVAES
                     NDQLMEKYFEQGTLDEADILPNINNAIAQSKLCPVFAVSSLTQVGLRALLNGLVDYAP
                     DPAHHEAEWGRQVDAGPDAERISRKYSDSEPFSAYCFRTIADPFAGRINVMKIMSGHL
                     QTDANAFNSTRGGVERLGALHLMQGKQLDKVPEAHAGDIVACVKLKETQTGDTLCDKQ
                     SPILYDPVEYPEAAIAFAIEPKSRQDEEKISVALHKILEEDPALHFSRDAQTKEFLLS
                     GSGQLHVETVVEKLKKRYSVEVTLHPPKVPYKETITQRAEVQGRHKKQTGGRGQFGDC
                     KCVFEPLPRGSGFVFEDKIFGGSVPQQFRPAIEKGIIEAAKSGAIAGYPLVDFKVQLV
                     DGSYHPVDSDEHSFRAAGRKAFRAAMEKVKPSLLEPVMDVEIAAPQEFSGDIMGDLNS
                     RRGRVQGMETSGKQQLIKAQVPMAEMLNYQSTLNSITAARGSFHMQFSHYDPVPGQLA
                     HKIVEQSRAEGRIRGGEEEE"
     gene            3140..>3439
                     /locus_tag="VFH91_01230"
     CDS             3140..>3439
                     /locus_tag="VFH91_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861627.1"
                     /translation="MYYRKILSLTGSLFLLFATLGVGYAKPVTKTDNEAQPRTQTIEP
                     INLAILIQDDLVSQVSNEIGVTKVFIRSLPAGSSVMVGYITAGSLQVRQPFTTDLD"
BASE COUNT          777 a          891 c          861 g          910 t
ORIGIN      
        1 tcctgcacca aatgcgccac cactggaaga aggacaacgg tttgaattca aggttgtcgg
       61 tcctggatct ccggccggtg gccctcaagt catgactgct ccgctgccgc ctggtgcgtt
      121 gcccggtgga gagaacgtaa cgttaagtac cgcaggcgcc gttggcggtg cgacttacgt
      181 attcagacgg aaaggcactg acgccaatga agtcaaagaa gatctgggcg aacagttgat
      241 tgaaggagtc aaggcacagg gaactcgttc gaccgtcact atccctgccg gtgagatcgg
      301 taatgatcgt cccatcgaaa tcgttagtga acgttggtat tcacccgaac ttcaaaccgt
      361 ggttatgact cggcacaacg acccaagaac tggcgaaacc acgtacaagc tcaccaacgt
      421 caatcgcaac gaaccagcga agtcattgtt tgaggtgccc gctgattaca cggtgaagga
      481 aggtttgccg ggtgttcctg gcgttcaagt agctcccgcg cgtgttcgta aatccaatat
      541 ccctgaatag caatcgtgcg agggcttgca taaaagtaaa tttagaagcg cggcggtgcc
      601 acgctcaatt ttccgctgtg attctcacga tggcgttatt gagcgtgaca ccgccgcgct
      661 tcgctcgcgg tacggtactg gtcgcgtctt actttttgtg cacagcacgt tgcggcagag
      721 tctaattgtc ataatgccgc gtttgtgtgg gcgcccctac tttttcgatt tctattattc
      781 ttcttcctca ccgccacgga tccggccttc agctcgcgat tgttcaacta tcttgtgggc
      841 caactgaccc ggaactggat cgtagtgcga gaactgcatg tgaaacgagc cgcgcgccgc
      901 ggtgatcgag ttcaatgtcg attgataatt gagcatctca gccatgggga cctgcgcctt
      961 gatgagctgt tgcttgccac ttgtttccat accctgcact cgacctcgtc ttgagttcag
     1021 gtcgcccatg atgtcaccgc tgaattcctg tggcgctgca atctcaacgt ccataactgg
     1081 ctcaagtaac gaaggcttta ctttctccat tgcagcgcga aatgctttgc gcccagcggc
     1141 tcggaatgaa tgttcatctg aatcgaccgg gtgatacgaa ccatcaacca gttggacttt
     1201 gaaatctacg agcgggtagc cagctattgc tcctgacttc gctgcctcga tgattccttt
     1261 ttcaatggcg ggacggaatt gttgggggac tgagccgccg aagatcttgt cttcaaaaac
     1321 aaagccactt cctcgtggta acggttcaaa gacgcacttg caatcaccga actgtcctcg
     1381 tccaccggtc tgtttcttgt ggcggccctg aacttctgct cgctgcgtga ttgtctcttt
     1441 gtacggtact ttaggaggat gtagtgtcac ctcaacgctg tagcgcttct taagtttctc
     1501 caccacagtc tcgacgtgca attggccaga cccggaaagc aggaattcct tggtctgagc
     1561 atcgcgtgaa aagtgcagtg ccggatcttc ttccagaatc ttgtgaagag ctacgctgat
     1621 cttctcttcg tcctgacgcg acttgggctc aatcgcaaaa gcaatggctg cctcagggta
     1681 ttccaccgga tcgtagagta tcgggctctg cttatcgcat agcgtgtccc ctgtttgagt
     1741 ctctttcagc tttacgcaag caacgatgtc gccggcgtga gcttcgggaa ccttgtccag
     1801 ctgttttcct tgcattagat ggagtgcgcc caaacgttca acgccacctc gtgtggagtt
     1861 gaaagcattc gcatctgttt gcagatgtcc gctcatgatc ttcatgacat tgattcgacc
     1921 tgcaaaagga tccgcaatag tgcggaagca gtatgcagaa aacggttcac tgtcactgta
     1981 cttgcgtgag attcgttcag cgtccggacc cgcatcgacc tgtctgcccc attcagcttc
     2041 gtgatgagcg gggtctggtg cgtaatcaac caggccatta agtagggcgc gcagtccgac
     2101 ctgcgtcaga cttgagacag caaagacggg acacaactta ctttgtgcga tagcgttgtt
     2161 gatgttcggt aaaatgtctg cttcgtcaag tgttccttgt tcgaagtact tctccatcaa
     2221 ctggtcgttt gattccgcca ctaactccac caatcgttcg cgagtcttat caaccaggtc
     2281 tcgaccttct gccggaatat cgatctcctt cgcctgtcca tcagcgccga gctcataagc
     2341 cttcatatgg accacatcca cgacgccgcg aaaatctttt tcttttccga tcggtaaagt
     2401 gaagggaatg attgccctac taaaagtgtt ttgagcactc tgcagcgcca ttccaaagtc
     2461 agcatgttct ttatcaagct ttgtgatcac cataacgcga gggaccataa actcattggc
     2521 ataggcccat gttttttctg tctgaacttc gacacccttg acgccgtcta caacaacgat
     2581 cgcgcagtcg gctacgcgaa gtgctggtcg agcgtgcgct atgaatgcgg cgtatcctgg
     2641 tgagtctatt aaattcagtt tcgtatcgcg gtgttcaaga tgggccagtg ccgtatttag
     2701 tgtgatcttt cgagcgatgg aatcttcttc atgatcagtg actgtcgtgc cttcatcaac
     2761 cttaccccag cgtggtgtgg ctccagcgac gaagagaaga gagctaataa gctgtgtctt
     2821 ccctgcgtcg ccatgtccaa tgacagccag gttccgaatg gcttcagtcg caaagacttt
     2881 catacgtgtg attcctttca ggcatgcgaa ttttggcgaa ggatacccat ccgctttgat
     2941 cgaaacgttt cggcagactt tggcgggatg tgctccgtga tttggctgta atttatctga
     3001 cgctcacggt taaacgcaag ttggtctatc tctgatgatg catccacgaa taacgaagca
     3061 ttttggccat ctttgttgac tcttacagca tcacaaagtc aggtgaaagg ggctctaaag
     3121 gctagagccg gaaaaagtta tgtattaccg aaaaattttg tctctaactg gatcgttatt
     3181 tctactgttt gccacacttg gagttgggta tgctaaacca gtaacgaaaa cagataacga
     3241 agcacaacca cgaactcaaa caatcgaacc tatcaatctt gcgattttga ttcaggatga
     3301 cctggtgtcg caggtgagta atgaaattgg agtaacaaag gtctttattc ggtcacttcc
     3361 ggccggctcc agcgtgatgg tgggttacat caccgccggc tctcttcagg ttcgacaacc
     3421 atttacgacc gacttagat
//
LOCUS       DASSFP010000061         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1057455, whole genome shotgun sequence.
ACCESSION   DASSFP010000061 DASSFP010000000
VERSION     DASSFP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057455"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFH91_01235"
     CDS             complement(<1..526)
                     /locus_tag="VFH91_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET6861628.1"
                     /translation="MKYSVLKSLLNRRIHERVRGGYRLCKSLTRNMGSVPIRIDSYPP
                     LYVDLRGLDAHALKLFTEEPLPSVPHEPALTRVFNEIIRPTDVVFDVGANLGLHSLTF
                     SQMAQKVIAFEPNPSLVPNLRKTLAGIPNAELQEFCLSDHDGSVDFHVSKWDHMLGSL
                     ADWSGQPADTITVPA"
     regulatory      1312..1408
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1536..1835)
                     /locus_tag="VFH91_01240"
     CDS             complement(1536..1835)
                     /locus_tag="VFH91_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861629.1"
                     /translation="MNNPLFGENKNMKEGLRVLTATFIADNNSQTGQGDLFAFHVSCM
                     KVLSRTYANTLVAETMRQTRRAQPGFETDIFCGKKGRNDRGTEFRLLLMMRGKSA"
     gene            1834..>3371
                     /locus_tag="VFH91_01245"
     CDS             1834..>3371
                     /locus_tag="VFH91_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861630.1"
                     /translation="MISHGRRMTPVSPVLQKVLSFVLSALILSTSIPFTAAISVSAQS
                     TVDVVATMPSPEVAFLVGPQVRSGTSSQSGAGWFDQNATNRGLQLCSEFPDVAHTPGN
                     ILISGTVSVTNGSKTVTGAGTHFLTEAKDYAIISNGSQGRLVKIVASVQSDTQLTLTS
                     TWQGATASGQTIASPTSASVDNYQGYLNYYDFAFTQYTNYYRTGDQRFLDCGRKVADS
                     WWSQPVIDYGRNLMSVTGDGMAPRSIALTGLILRALDGRPEMWPWITDYVNYQYHNWV
                     EVPMNWSGLYFGIRDGGFMLLYAADLGAVHPDPTVRQSFKTRALAGAVNYYSRLQNTD
                     GSYRWSVDDSSPGSLDGFTGMEQPFMVGILNEGMIATHRLTGDATVANAIKKSVEHEY
                     MRSYNPNGWRGMYYFVHGQFNTGFSCESGCGSASNPYPPSDTSLISETRQLSATAISQ
                     FGYAYMISGDFRYKQWGEEIFDATYSGTDGYRGLAAYRGKEYDESYRSGGKFLAWRLG
                     GTFPT"
BASE COUNT          854 a          806 c          833 g          878 t
ORIGIN      
        1 aggccggcac agttatggta tcggctggct gtccgctcca atcagccaga ctgcccaaca
       61 tgtgatccca tttggaaacg tgaaagtcca cgcttccgtc gtgatcactc aaacaaaact
      121 cttgaagttc agcgttgggt atgcccgcaa gagtcttccg aaggttcgga acgagagaag
      181 gattaggttc aaacgcaata accttctgcg ccatttgaga aaacgtgagg gagtgaaggc
      241 ccaggttggc acctacatca aacactacat cggttggacg aataatctcg ttgaagactc
      301 tggttaatgc gggctcgtgc ggtacagatg gtaaaggttc ttctgtaaaa agcttgagtg
      361 catgtgcatc gaggccacgg agatcaacgt agaggggtgg gtaggaatct atcctaatcg
      421 gaaccgaacc catgtttcga gtcaaactct tgcacagtcg gtatcctcct ctgactctct
      481 cgtgaatgcg tctattcaat agcgacttaa gaacagaata cttcaccctt gagtcctctg
      541 gatgactgtg gccgtcatcg cgtctttgtg cgccttactg aggatcgctt ctcgttcttc
      601 ttgtcgaaat aggaatttag aaagatagca aaagccaaac tccctccgag aaaagcaatt
      661 gtgagaagtg tcatcatgac tctttattcg aaaagcgcct caaacttgct actagtgaaa
      721 gacaataaca caacgaacaa tcttcgttta aaaactaaat atttcgtcgt caaatactga
      781 acacaaaata accacaactt ttctacaacc tttaatccaa tgtgatggag ttaaatcatg
      841 agtgtctgag ctgttcaata agtcagtccc ttcaagtaag taaaaataca ccagaaagaa
      901 gaagtaatag ccgggatggc taaaatcagg aagtagtgtc cctagagccc agagtttgtg
      961 acaaattttg tcaggaacgc ctcgggggca cttttcaaaa cgcgcgcgaa aactagacaa
     1021 acaacccaga tagttggcat ccacttcgtg tactgaacac cctccgctgt gcggcaaaat
     1081 gagggaagtt cagagtcaaa actgaggtct cgtgcggcga ccgttctaag ataatttccc
     1141 ggacaaaatt gagcgaattg cttggccatt ttgccccatc gatctgctca ttttgcccaa
     1201 caaagttgaa aaagaaaagt cattgacaat acaaggaggg cggtgtttaa tgggcgtacg
     1261 tttggtcgcg aaagacttcg cggcactgat cttttataac gtgacgattg cacacgataa
     1321 aggcaaacct gtcgcgagac agggacgcaa agccacgagt cttacagaat ggtctcgatt
     1381 tactctgaaa gatggtcggg ttgccgaagg cgatctgaga gagtcaagtc tccactgttt
     1441 gactatttgg cttcggcgac gtgttgcacg ttacgcttgc ttcacggctc gcctgagtca
     1501 actccagaac cctaaaagtt ttggagctgt actggctaag cgcttttgcc tcgcatcatc
     1561 agcaaaagtc ggaattcagt acctcgatcg tttcttcctt ttttcccaca aaaaatatcc
     1621 gtctcgaaac ccggctgggc tcttcgtgtc tgtcgcatgg tttctgcgac taaggtgttc
     1681 gcgtatgttc ggcttaatac cttcatacat gaaacatgaa acgcgaacaa atcaccttgt
     1741 cccgtctgtg agttgttgtc ggcgataaac gtagccgtca aaactcgcag tccttccttc
     1801 atatttttgt tttcaccgaa aagaggattg ttcatgatct cccatggtcg acgcatgact
     1861 cccgtgagtc ctgttcttca aaaagttctt tcgtttgttc tttccgctct catactaagt
     1921 accagtattc cgtttacggc cgctatctcg gttagcgcgc agtcgactgt tgatgttgtt
     1981 gccacaatgc catccccaga ggtcgctttc ttagttggac ctcaggttcg aagtgggacg
     2041 agcagtcagt cgggtgcggg atggtttgat caaaatgcta cgaatcgagg acttcaactc
     2101 tgcagtgaat tccctgacgt ggcccatacg ccaggtaata ttttgatctc gggtactgtg
     2161 agtgtcacga acggtagcaa gactgtcaca ggtgcaggaa cgcacttttt aactgaagcc
     2221 aaagattacg ccattatttc caacggctca cagggccggc tggttaagat agtcgcttca
     2281 gtgcagagtg atactcagct cacgctgaca tcgacctggc aaggtgccac cgcgagcggt
     2341 cagacgatcg cttcgccgac cagcgcttcg gtggataact atcagggcta ccttaactac
     2401 tacgatttcg cctttacgca atacacaaac tactaccgaa ctggcgatca gcgctttttg
     2461 gattgtggac gaaaagtagc cgattcgtgg tggtcgcagc cagtcatcga ttacggccgg
     2521 aatctgatgt cggttaccgg tgatggcatg gcaccgcgaa gcattgcttt aacaggtctg
     2581 attttgcgag cactcgatgg tcgtcctgaa atgtggccat ggattacgga ttacgttaac
     2641 taccagtatc acaactgggt agaagtgccg atgaattggt ccggtcttta ctttggtatt
     2701 cgagacggcg gcttcatgtt gctttatgcg gcggatcttg gagcggttca tccggatccg
     2761 acagtgcgac agtcctttaa gacaagggcc ttagccggtg cggtcaatta ctattcccgc
     2821 ttgcaaaata ctgatggctc ttaccgctgg agtgttgacg actcaagtcc cggctcgctc
     2881 gatggtttta ccggaatgga gcaacctttt atggttggca tcctgaacga aggaatgatt
     2941 gccactcatc gtttgaccgg agatgccacc gtcgcgaacg caatcaaaaa atctgttgag
     3001 catgagtata tgcggagtta caacccgaac gggtggcgcg gcatgtacta ctttgttcat
     3061 ggtcagttca atacgggttt ctcgtgcgaa tctggttgcg gtagcgcttc gaatccttat
     3121 cctccctcag atacaagttt gatctcggaa actcgccaac tgagcgctac agcgatttcg
     3181 caattcggtt atgcgtatat gatttccggc gacttccgtt acaaacaatg gggcgaagag
     3241 atttttgatg cgacctacag cggtacggac ggttaccgcg gccttgccgc ttatcgtggt
     3301 aaggaatacg acgaatccta tcgcagtggt ggaaagttcc tcgcatggcg attgggtggt
     3361 acgttcccga c
//
LOCUS       DASSFP010000062         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_462054, whole genome shotgun sequence.
ACCESSION   DASSFP010000062 DASSFP010000000
VERSION     DASSFP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462054"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFH91_01250"
     CDS             <1..579
                     /locus_tag="VFH91_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6861631.1"
                     /translation="NLATEMGEMQERITSTKTGSVTSIQAVYVPADDYTDPAPATTFA
                     HLDAVTALSRQIVELGIYPAVDPLGSSSRILDPRILGEEHYDTAQDVKRVLQRYKDLQ
                     DIIAILGLDELSDEDKLIVSRARKIQRFFSQPFHVAEQFTGLEGKYVKLEDTVRGFRE
                     LVDGKHDDLPEQAFYMVGTIEEAIEKAKTFAQ"
     gene            632..1042
                     /locus_tag="VFH91_01255"
     CDS             632..1042
                     /locus_tag="VFH91_01255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET6861632.1"
                     /translation="MADKIQLEVVTPERRVLAEPVDMVTMPGLGGELGILPGHTPLIS
                     QLKTGVLTYVQDGKSFQLHVSGGFLEVRDDHVSVLAEVAERPEEIDAAQARQSRERLE
                     KQLNNWSGSEEDLAVAQAELERSTVRLQLASSRA"
     gene            complement(1052..1834)
                     /locus_tag="VFH91_01260"
     CDS             complement(1052..1834)
                     /locus_tag="VFH91_01260"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET6861633.1"
                     /translation="MTARLNVNIDHVATVRQARRAPEPSVIAAAMACELAGADGITVH
                     LRGDRRHIQDQDLSLLRQSVSTYLNMEMAATEEMLKVALENRPDAVTLVAEKPDEITT
                     EGGLDVQANIAIVRAAVSKLRQGEILASLFIDPDPRQIEVAHDVGAQQVELCTAAYAE
                     ATLGARAIHGEGAAHAAQELRRLRDAAELATQYGLHVAAGHGLTYRNVGAVAGISEIT
                     EFNIGHNIVSRAIFVGMEKAVYEMRKAIKAGSFRSGLGNVSD"
     gene            complement(1921..2691)
                     /locus_tag="VFH91_01265"
     CDS             complement(1921..2691)
                     /locus_tag="VFH91_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HET6861634.1"
                     /translation="MPTSLSIIVPAYNEADRLGNSLGAIVTYLRDNFPDAELIVVDDG
                     SSDKTAQIARQRLDEGGRLRTSLISYKSNLGKGRAVRLGLLAARGDIALFSDADLSTP
                     ISEAPKLIDPIENGECDVAFGSRALDRDLIGVRQPWRREQGGRVFNLVVRLATGLPFW
                     DTQCGFKAFRMSVCRPLLEAATVDRFGFDVELLYLAYKAGLRLKEIPVRWDHNEGSKV
                     NVASDSFKMLSEVGLIRQQARRGVYDNALKAVAETKRS"
     gene            2778..>4040
                     /locus_tag="VFH91_01270"
     CDS             2778..>4040
                     /locus_tag="VFH91_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6861635.1"
                     /translation="MEVARELESSRTQYEGIGLSKRFVLIAAAVVVLAAVFGGTLGSA
                     RKFSGLNGGGKSNPYRVVDEIEKDYNEAVSTITSNYSGEIDHEKATQAAIQGMLSTLD
                     PHSAYFPYNEFKKLKEDQDSRFFGIGVTIVQHHDGVYIQSTVEGTPAARLGLKYGDRI
                     LEVDGKDARQWTSDLVSKNVRGGIGEPVTIKVERAGSEAPQYFTLVRDAVPLLTIRNA
                     YIIRPGTGYIGLTGGFQRSSDDELRKAIKKLEEQGMRQLVLDLRSNPGGLLDQAIDVA
                     SEFLPRGKVIVSVKGRTEYNEPVIYKSTGAEPSDVPLVVLINRNTASASEIVAGAIQD
                     HGRGLIVGETSFGKGLVQRIFQLPYNTGLTLTTARYYTPYGRSLQRDYSNGSLYDYYT
                     RHDPSEMALPNQGTRNLELPLALASPTPH"
BASE COUNT          958 a         1060 c         1054 g          968 t
ORIGIN      
        1 aacctcgcaa ccgagatggg tgaaatgcag gagcgcatca cctctactaa aactggttca
       61 gttacgtcga ttcaagcggt ctatgtaccg gctgacgact ataccgatcc cgcgccggct
      121 acaacctttg cgcacctcga cgctgtaact gcgttgtcac gtcaaattgt agaacttgga
      181 atttatccag cggtggatcc cctgggttct tcttcccgaa ttctcgatcc acgaattctg
      241 ggtgaggagc actatgatac cgcccaggat gtgaagcgtg tgctgcagcg ttacaaagac
      301 cttcaggaca tcatcgcgat tctcggactt gacgaactgt cagacgaaga caagttgatc
      361 gtttcacgtg cgagaaaaat ccagcggttc ttctcgcagc ccttccacgt ggcagaacag
      421 tttactggcc tggaaggtaa gtatgtgaag ttagaagata cagtgcgcgg attccgtgag
      481 ctggtagatg gtaaacacga cgatctacct gaacaggctt tttacatggt gggtacgatc
      541 gaagaggcca tcgaaaaagc taagacattc gctcagtaac tcttgaaagc gggccgcgtc
      601 agatgagcgt ggcccaataa cttgttcctt tatggccgac aaaatccaac tcgaagttgt
      661 tactccggaa cgccgtgttc tcgctgagcc cgttgacatg gtcactatgc ctggtcttgg
      721 cggagagctc ggcatccttc ccggtcacac gccgttaatc tcgcagttga aaacaggtgt
      781 gctgacatat gttcaagacg gaaagagttt tcaattacac gtctcgggtg gttttctgga
      841 ggtcagagac gatcacgtgt cagtcctggc agaagttgcc gaacgacccg aagagatcga
      901 cgcggcacaa gcacgacaat ctcgcgaacg cctcgagaaa caacttaata attggagtgg
      961 ttctgaagag gatttggcag ttgctcaagc tgagctggaa cgctcaacgg tgcgattgca
     1021 gttagcctcg tcgcgagctt aaaaacagat ttcagtcgga gacattgccg aggccggatc
     1081 gaaatgatcc ggcctttatt gctttgcgca tctcgtaaac tgctttttcc attcctacaa
     1141 aaattgcgcg agaaacaatg ttatgtccga tattgaactc tgtaatttca gatatacccg
     1201 caacagcgcc aacatttcgg taggttaatc cgtgaccagc ggctacgtgt aaaccgtact
     1261 gagtggcgag ctccgccgca tctcttaatc ttcttaactc ctgggcggcg tgagctgcac
     1321 cttctccgtg gattgctcgc gcaccgaggg ttgcttcagc gtaggccgcc gtacaaagtt
     1381 caacttgttg agccccgacg tcatgggcca cttcaatttg tctgggatca ggatcgataa
     1441 aaagactcgc aagtatttcc ccctgacgca gcttcgaaac tgccgcccgc acaatcgcga
     1501 tgttcgcttg cacatcaaga ccaccttctg ttgtgatctc gtctggtttt tccgccacca
     1561 gggttacggc gtcaggtcga ttctcgagcg ctaccttcag catctcttca gtcgccgcca
     1621 tttccatgtt caggtacgtg gaaacagact ggcgcaagag ggagagatcc tgatcctgaa
     1681 tatggcggcg gtcgccgcgc aggtgcactg tgattccgtc ggctcccgcc agttcacagg
     1741 ccatagccgc agcgatcaca cttggttcgg gcgcgcggcg cgcctggcgt accgttgcca
     1801 catgatcgat gttcacattg agccgagcag tcattccatc accagagttg aaccagtata
     1861 agcgttacag aaccgtgagg cgaagctact gttaagtttc aagaacgcgg ttgggtgctg
     1921 ctaacttctc tttgtttcgg ctacggcttt gagagcatta tcgtaaaccc ctcgccgggc
     1981 ttgctgcctg attaatccaa cctcactcaa catcttaaaa ctgtcgctcg ccacgtttac
     2041 cttgcttcct tcattgtggt cccatcgaac aggaatctct ttcaatctga gtcccgcttt
     2101 ataggcgagg taaagtagct cgacatcaaa tccaaaccgg tcaacggtgg cggcttccag
     2161 caaaggacga catacgctca tccgaaacgc cttgaaaccg cattgggtgt cccaaaaggg
     2221 aagtccggtt gcgagtctca ccacaagatt gaagactctg ccaccctgct cacgtcgcca
     2281 gggttgtcgt acaccgatca ggtcacggtc gagtgcccga gatccaaatg ccacatcgca
     2341 ctctccattt tcgatcggat cgatcagttt tggagcttcg ctgatgggcg ttgaaaggtc
     2401 cgcatctgaa aatagtgcga tgtctccccg ggcagccagt aaaccaagtc gtactgcgcg
     2461 acctttgccc agattcgact tgtagctaat taaagaggtg cggagacggc cgccctcgtc
     2521 cagccgctgg cgggcgattt gggccgtctt atccgacgag ccatcatcga cgactattaa
     2581 ctcggcgtcg ggaaagttgt cgcgaagata ggtgacgatt gcgcccagac tgtttccaag
     2641 tcgatcggcc tcgttataag cgggaacgat gattgagagc gatgtgggca tctgtgattc
     2701 tcaatcgcaa tatatcacag tcaaaactcg tcgcggccgt gtcggaggcg agcctttgcc
     2761 tgtgttatat ttcaggcatg gaagtagcac gtgaattgga atcctcaagg acacaatacg
     2821 agggaattgg tctcagtaag agatttgttc tcattgccgc agccgtggtg gttttggctg
     2881 cggttttcgg cggcacactc ggtagcgccc gaaagttcag cgggctaaat ggcgggggga
     2941 aatccaatcc ctatcgagtc gttgatgaaa ttgagaagga ttacaacgaa gccgtctcga
     3001 ccatcacctc aaactattct ggtgagattg atcacgaaaa ggcgactcag gctgccattc
     3061 agggaatgct ctcgaccctg gatccacatt cggcttactt cccctataac gagtttaaga
     3121 aactgaagga agatcaggac tcgcgctttt ttggcattgg ggtcaccatc gtgcagcacc
     3181 atgatggtgt ttacattcag tctacagtgg aaggtacacc tgcagctcga ttaggtctga
     3241 aatatggaga tcgtatcctc gaagttgatg gcaaggatgc gcggcagtgg accagcgatc
     3301 tggtttccaa aaacgttcgc ggcgggattg gtgaaccagt tacgatcaag gtcgagcgtg
     3361 cagggtccga agctcctcaa tatttcacac tcgtaagaga tgccgttcct cttttgacga
     3421 tccgcaacgc ctacatcata agaccaggca ccggctacat cgggttgact ggcggattcc
     3481 agcgttcaag tgacgacgaa ttgcgaaaag cgatcaagaa gttggaagag caaggtatgc
     3541 gccaacttgt gctggatctt cgcagtaatc cgggcggctt gttggaccag gcgatagatg
     3601 tcgccagcga atttctgcct cgcggtaaag tcatcgtttc ggtaaaagga cggaccgaat
     3661 acaacgagcc ggtaatctac aagtctacgg gcgccgaacc ctctgacgtt ccgttggtcg
     3721 ttctcataaa tcgcaacact gcctcagcct ccgaaattgt tgcgggggcg attcaggacc
     3781 atgggcgggg gcttatcgtt ggtgaaacta gctttggaaa aggcctcgtg cagagaatct
     3841 tccagcttcc atataatacc ggactaactc tgacgactgc ccgttactac acgccatatg
     3901 gacgctctct tcaacgtgat tactcaaatg gatcgcttta cgactattac acgcgtcacg
     3961 atccttctga gatggccctg ccgaaccagg gaactcgcaa tctggagcta cctctggctt
     4021 tagcatcccc aacgcctcat
//
LOCUS       DASSFP010000063         5929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1951972, whole genome shotgun sequence.
ACCESSION   DASSFP010000063 DASSFP010000000
VERSION     DASSFP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951972"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..1546
                     /locus_tag="VFH91_01275"
     CDS             422..1546
                     /locus_tag="VFH91_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6861636.1"
                     /translation="MSNSLPRILIVDDESEITEILADLLSETYLCSKAGTAEQALICL
                     QEQEFELVISDITMPGMSGLDMIPHVKEISPNTVVVMISGMQTVESAIGALRLGAFDY
                     LMKPFDLRQVEAVVKRALEHHELIAAKQRYENHLEELVEQRTVELDRALDSLEDAYRS
                     TLRALTAALETRDSETHGHSERVVSYSLRLGREYGLSREEMKALEFGSLLHDIGKIGV
                     PDSILRKPGKLTDEEWVRMKEHPLHGQLILRGIEFLRGAARVVAQHHEKWDGSGYPLG
                     LHETEIDVCARIFSVADAFDAITSDRVYRRGKSYEAAAQELDEWAGRQFDPKVVEAFH
                     RVPKEDWEELHRLSLLPKHDEFDVKQMVQMLEAQLEAAVR"
     gene            complement(1550..2707)
                     /locus_tag="VFH91_01280"
     CDS             complement(1550..2707)
                     /locus_tag="VFH91_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6861637.1"
                     /translation="MPLLYFKTTLAILLALLVISASQSTSSQTKSTQSPSPTPTPVTQ
                     EPDQERVRVFTEEVRLPIIALDAYGHYDPTLEPDDVLVLEDGVTQQIRSVRHIPANVV
                     FLLDTGGESSGLGGLSKSTALTRLVATDLIQRLKDDSWIDVVQAGNSTNVVQPWTQDR
                     QTVIKTLKTKLLATKLSRFSDAVLTAAQQLASRPEGSRHVVMITDGVDSPGSKTNRAD
                     AIKKLIAARATIHIISYTELVRQKGDEKSSKITTGQRPTTSDPITATDPTLPPGTTRS
                     PSYGVSIRFDPAMRKVRKAYEADVKKSQQELTSLADETGGKIYLPISSDEMRSRATEV
                     ARAIEAEYVVTYRPTKPLSEARPGEYRKIDVTSRRVGLSLYSRRGYVVPGE"
     gene            complement(2792..3799)
                     /gene="pta"
                     /locus_tag="VFH91_01285"
     CDS             complement(2792..3799)
                     /gene="pta"
                     /locus_tag="VFH91_01285"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET6861638.1"
                     /translation="MNILQHIRQRAASRPQHIVLPEGNDSRTVIAAAECVNQRIAQIT
                     LLGDEAVIRDTASANNLSLGGVTIMDHRRAPDFEKMTSLFHELRRAKGLLADEARTLV
                     SNPLYYGNLMVRAGKADGSVAGATNTTAQTVRAALHCIGLRQGFKLVSSFFLMSLQNR
                     DFGHDGALIFADCGVVIDPTAAELAEIAIASADSCKALLGCPPRVAMLSFSTKGSAHH
                     KLIDKVVEATRTAKARAPELVIDGELQADAALLPTVANSKAPGSAVGGKANVLIFPDL
                     QSGNIGYKLTERLAGATAIGPILQGLDKPANDLSRGCKPQDIVDAVAITAVQAQARKA
                     I"
     gene            3960..4421
                     /locus_tag="VFH91_01290"
     CDS             3960..4421
                     /locus_tag="VFH91_01290"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET6861639.1"
                     /translation="MASSMLQSRMKNLLLFVPNLVVLCGKLMVDPRVPAKERVLVAGA
                     IVYAIVPLDLIPDMIPFVGQIDDAYLIALTLLRLMTISEPAVVREHWRGGGDVVELIG
                     STALISAKFLPKRIRRVLTSRVEAPRANGSSKSMAPILIERPQVDSNSKGD"
     gene            complement(4436..5002)
                     /locus_tag="VFH91_01295"
     CDS             complement(4436..5002)
                     /locus_tag="VFH91_01295"
                     /inference="COORDINATES: protein motif:HMM:NF021577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HET6861640.1"
                     /translation="MHNFSIFEQALQISPPELTENLGQLVLISRRDFNERESFLCRTT
                     TSSLEYACLMIENALLLHTNRNGRVYAGFERLSYFQPIMDRYSRIADLSDSVYLFGQD
                     DWRPPRHPNIRVITLDPDARLAKESFLITQSTTFNCALIAFEPPGEESVLRLGESLNY
                     WLLKTSNGAVVGQLSNALERLIDVTLAA"
     gene            complement(5126..>5929)
                     /locus_tag="VFH91_01300"
     CDS             complement(5126..>5929)
                     /locus_tag="VFH91_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET6861641.1"
                     /translation="VKLAFRRFMNLVPANGKLIAGWDSPNVRAVVDSMGKKLFTTLET
                     FGTTSEAKWQIREPRFGASLSEFKVFRAGELWGEFRTPLLGGFNLLNCLAVIVAADTW
                     GVTKEKISDALETFKNVKRRAEVRGEKRGIIVIDDFAHHPTAVRETLRALHARYEGRR
                     LVAVFEPRSWSSRLAVFQKDYADAFTAADYVVIASVFDSQKVTEKGRALNTAELIEDI
                     SKQGKPAFAFADVEQILSHLVPDLREGDVVAIMSNGGFGGIHDKLLAVL"
BASE COUNT         1431 a         1467 c         1539 g         1492 t
ORIGIN      
        1 tttttcattt ttcatttcga ctgaaccggc tgcgtacgaa aggttaaatt gcaaatcgca
       61 actatcttca tacctttccc aactggactc tgccggaaat gagaaatgga aaatgagaaa
      121 tggaaaatga gaaatggaaa atgggaaatg acgtcgccat ctcattttgg acctgttcga
      181 gacaaactcc tccgaaaatg taccgagtgc cggtcgctaa gatactgtaa accaagagct
      241 tacatccttc cgtaaaaaca tgtttgccac tcccaaggga aaagggctaa aatgccagag
      301 cctttaaaag gctgagggac ctgaccccgc ctgagtcatc tcacccaagt aagttaattg
      361 agttctacca atatcctgtc ggacgtgcag cggcgtcttg tcacgtttca ggagaaacca
      421 aatgtcgaac agtctgcccc gaattctgat agtagatgac gagtcggaaa ttaccgaaat
      481 actcgccgac ctgctaagcg aaacatacct gtgttcaaaa gcaggtaccg ctgagcaagc
      541 cttgatctgt ctccaagagc aagaatttga actcgttatc agcgatatca caatgcctgg
      601 catgagcgga ctcgacatga ttccgcacgt caaagaaatc tcaccaaaca cggtggtcgt
      661 catgatcagc ggaatgcaga ccgtcgagag tgccatcggt gcgcttcgat tgggtgcatt
      721 cgactatttg atgaagccct tcgaccttcg tcaggttgag gcagtcgtaa aaagagcact
      781 cgagcatcat gagttgattg ctgccaagca acgttacgaa aatcatttag aggaactagt
      841 ggaacagcgt acggtcgaac tcgatcgagc gttggactcg ctcgaagatg cttatcgatc
      901 gacattacgc gcactgacag ctgctcttga gactcgtgac tcagagacac acggtcactc
      961 agaacgcgtc gtgagttata gcttgaggct cggtcgtgag tacggtctta gtcgtgaaga
     1021 gatgaaggct ctggaattcg gatccttgct ccacgatatt ggaaagattg gcgttcctga
     1081 ttcaatccta cgaaaacccg ggaagctcac cgatgaagaa tgggtccgca tgaaagagca
     1141 tccgttacac ggccagctga ttcttcgcgg tatcgaattt ctgcggggcg ctgctcgtgt
     1201 ggtggcccaa catcatgaga agtgggatgg ctctggttat cctttgggat tgcacgagac
     1261 cgaaatcgat gtctgcgccc gtatcttttc tgtggccgat gccttcgacg cgattacaag
     1321 cgatcgtgtt taccgtcgtg gcaaatcata tgaagctgct gctcaggaat tagacgagtg
     1381 ggcaggaagg caatttgatc caaaggtggt ggaagctttc caccgagtgc ccaaagagga
     1441 ttgggaggaa ttacatcgat tatcactact tcccaagcat gatgaattcg atgtgaagca
     1501 gatggttcaa atgctcgagg cacaactcga ggctgcagta cgttaagcgt tactcgcccg
     1561 gaacgacgta ccctcttcgc gaatacaaac tcagtccgac cctcctggaa gtcacatcaa
     1621 tttttcgata ttcacctggc cgcgcctccg acaaaggttt cgtcggtcga taagtaacta
     1681 cgtactcagc ttcgattgct cgcgcaactt cagtagcacg actacgcatc tcgtcgctgg
     1741 atatcgggag atagatctta cctccggttt catctgcgag actcgtcaat tcctgctgac
     1801 tcttcttaac atcagcctcg taggcctttc gcactttgcg cattgccgga tcaaatctga
     1861 tcgatactcc ataagagggt gaacgagtgg ttccgggtgg caaagttgga tcggtggcag
     1921 taatcggatc ggaagtcgtc ggacgctggc ccgtggtaat cttggatgat ttttcgtcac
     1981 ctttctgtcg cacgagttct gtatagctga tgatgtggat cgtggcgcga gcggcaatca
     2041 attttttgat tgcatccgca cgattagttt tacttcctgg agaatcgacg ccgtcagtga
     2101 tcatcacgac gtggcggctt ccttctggtc tactggcgag ttgctgtgct gctgtcagga
     2161 ctgcatcaga gaagcgagac agttttgtcg ccaacaattt ggtctttaac gtcttaatca
     2221 ccgtttggcg gtcctgagtc cacggctgaa ctacgttagt tgaatttcca gcttgaacca
     2281 catcaatcca cgagtcatct ttcagacgct gaattaaatc tgtcgcgacc agtcgagtga
     2341 gcgcagtgct tttcgaaaga cctcccaaac cacttgactc gccgcctgta tctaacaaga
     2401 acaccacgtt ggcgggaatg tggcgcacac ttctgatttg ttgggtcacg ccatcttcca
     2461 gtacgagaac gtcgtcaggc tcgagtgtcg gatcgtagtg tccataggca tcgagggcga
     2521 taatcgggag tcgcacttct tcggtgaaga cgcgaacgcg ttcctgatcg ggctcctggg
     2581 ttaccggcgt cggagttggt gacggcgact gcgtagattt agtttgtgaa gaggtcgact
     2641 ggctcgcgct aatcaccagt agagccaaca agatggcgag tgtggtttta aaatacagaa
     2701 gcggcatttc tacaaccgtg gagcaatacc cgggtaattc tagattgctg gtttgatgcc
     2761 cgcgctcgtc gtgagttgcc ctttgcgccg tctatatggc tttgcgagcc tgagcctgaa
     2821 ctgctgttat tgccacggcg tcaacgatgt cctgcggttt gcagccgcgg gaaaggtcat
     2881 tggctggctt atccagacct tgcaggatcg ggccgatggc tgttgcgcca gcgagccgct
     2941 cagtaagttt gtatccgata tttcctgact ggagatcggg aaaaatcaaa acgtttgctt
     3001 tgccaccgac tgcagatccc ggagcctttg aattagcgac cgttggaaga agcgccgcgt
     3061 ccgcttgcag ttcgccatcg attaccaatt caggtgcgcg cgcttttgca gtgcgagttg
     3121 cttccacgac tttatcgatt agtttgtggt gagcgcttcc cttggtagaa aacgacagca
     3181 tcgccacccg aggcggacaa ccaagcaaag ctttacatga atctgcagag gcgatggcga
     3241 tctccgccag ctctgctgca gtcggatcga tcaccactcc gcagtctgca aaaatcaaag
     3301 ctccgtcatg tccaaagtct ctgttttgca aagacattag aaagaagctc gacaccaact
     3361 taaatccttg tctgagacca atgcagtgaa gtgcagcccg gaccgtttgc gccgtggtat
     3421 tggttgcacc cgcgacggat ccgtccgctt tgccggcacg gaccatcaaa tttccgtagt
     3481 agagtggatt tgataccagc gtccgcgctt catcggccag cagtcctttg gctcgtcgta
     3541 attcatgaaa caacgacgtc atcttttcga agtccggagc gcgacgatga tccattattg
     3601 tcactcctcc gagactcagg ttgtttgcgc ttgctgtatc gcggatgacc gcttcgtctc
     3661 ctaacagggt aatctgagca atacgttgat taacacattc ggcggcggca attacggtgc
     3721 gcgagtcatt tccttccgga agtacgatat gttgaggtct tgaggcggcg cgttgtcgga
     3781 tgtgctgaag aatgttcatg ggttactgtt tacggacggt gcagtctagc caaacatatc
     3841 ggtgagaaca agtttgggcc tgcgcttttg cggactggtt attccagaca cttcttccga
     3901 ctgacccaat agtcgtgata ggtttacgtt gcaatgaaca gttagtcgac ggagagaata
     3961 tggcgtcatc aatgttacaa agccgaatga agaacctttt gctgtttgtg cctaatctgg
     4021 tagtgctgtg cggaaaactc atggttgacc ccagggtgcc tgccaaagaa cgggtactcg
     4081 ttgccggagc aatcgtctat gcaatcgtac cgttggatct tattcctgac atgattccat
     4141 ttgtgggcca gatcgacgac gcctatttaa ttgctttgac gctgctacgg ttgatgacaa
     4201 tatctgagcc tgctgtcgtg cgagagcatt ggcgaggtgg cggggacgta gttgaactca
     4261 ttggctcgac tgcgctcatt tcggcgaagt ttttaccgaa aagaattcgt cgggttctaa
     4321 catcgcgagt tgaggcaccg agagctaacg gaagtagcaa atcgatggcg cccattttga
     4381 ttgagcgtcc acaggttgat tcgaattcta aaggtgatta gctaagcgac aggaatcagg
     4441 ccgctaaggt tacgtcaatc aagcgttcga gagcgttcga cagttgtcca accaccgcgc
     4501 cgttgctcgt cttcaacaac cagtagttca gcgattcacc taaacgcaat acagattctt
     4561 ctcctggtgg ttcaaaggcg atgagtgcgc agttaaatgt cgtggattgc gtgatcagaa
     4621 aagactcttt cgccaaacgg gcgtcagggt caagtgtgat cactctgata ttagggtgtc
     4681 gtggcggtcg ccaatcgtct tgaccaaaga gatagacgga atcagagaga tccgcaatac
     4741 gtgagtaccg gtccattatc ggttgaaaat atgagagtcg ctcgaaacct gcgtacaccc
     4801 ttccgtttcg attggtgtgc aacaacagtg cgttttcgat cattagacaa gcatattcca
     4861 acgatgaagt cgtggtccga cagaggaagc tctctcgttc attgaaatca cgacgtgaga
     4921 tcaggaccag ctgtccaaga ttctccgtca gttcaggagg tgaaatctgt aacgcctgtt
     4981 cgaaaataga aaagttgtgc actgggacgc ctccaaggct tatgcggagg tctccatgta
     5041 ccggacttat cctgcaagac ggttacgaat ctttcgttaa agtgttgtgg agtaacaaaa
     5101 tggcagaatc ctgccaccgc gtttactaga gtactgccag aagtttgtcg tgaattcctc
     5161 cgaagccacc gttcgacata atcgccacaa catccccttc gcgaaggtcc ggaaccagat
     5221 gtgacaaaat ttgttccacg tcggcgaagg cgaaagccgg tttgccttgt ttcgaaatgt
     5281 cttcgattaa ctcagcggta ttgagcgcac gtcccttttc tgtgaccttt tggctgtcaa
     5341 agacgctagc gataaccaca tagtcagcag cagtgaatgc atcggcgtag tctttctgaa
     5401 agactgcaag tcgtgaggac catgaccggg gttcgaagac cgcaaccaac ctgcgccctt
     5461 cataccgtgc gtgaagagct cgcaaagtct ctcttacagc tgtcggatga tgggcgaagt
     5521 cgtcgatgac gatgattccg cgcttctctc cgcgtacttc agctcttcgc ttgacgttct
     5581 tgaaggtctc caaagcgtca gaaattttct ctttagtcac accccaggtg tctgcagcaa
     5641 ctatcaccgc cagacaattc agaagattga acccgcctag cagaggtgtg cgaaactctc
     5701 cccagagctc tcctgctcgg aacactttga actctgagag actcgctccg aacctgggct
     5761 ctcggatctg ccactttgct tcgctcgtag tgccaaaagt ttcgagggta gtgaacagtt
     5821 tcttacccat cgagtcgaca acagcacgta catttggact atcccagccg gcgatgagct
     5881 ttccgtttgc gggcactaga ttcatgaaac gacgaaaggc aagtttgac
//
LOCUS       DASSFP010000064         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1802989, whole genome shotgun sequence.
ACCESSION   DASSFP010000064 DASSFP010000000
VERSION     DASSFP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802989"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..859)
                     /locus_tag="VFH91_01305"
     CDS             complement(362..859)
                     /locus_tag="VFH91_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HET6861642.1"
                     /translation="MSSNQDNPVIPKKEVTIFCDGSSLSNGREVARAAAVALLGYKGT
                     WKAVGEYIGNATNQQAEIVAAAIGLEKLREPCRVKIFSDSRYVIETMSGRFRRKANHR
                     WWERLDNAAKPHRIEWAWTRGHVGHLVQEAADTAARRIAASGTVDESILRHAEDTVGS
                     GDRGK"
     gene            complement(860..1309)
                     /locus_tag="VFH91_01310"
     CDS             complement(860..1309)
                     /locus_tag="VFH91_01310"
                     /inference="COORDINATES: protein motif:HMM:NF042776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6714 family protein"
                     /protein_id="HET6861643.1"
                     /translation="MNWTQEAKRIFNVPKPEHFTDFRHCCECAEHDQVLVSSDVDSIG
                     VTELGNPGWDPICFCTSDGFLYYMPALIRLTLDTIDGNQYLDQFLFHLICDGKDNRRV
                     AACTIEQRKFVASFLEYLIENYTERIEEALCYSDDILKAHDIWKVET"
     gene            complement(1403..1861)
                     /locus_tag="VFH91_01315"
     CDS             complement(1403..1861)
                     /locus_tag="VFH91_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6861644.1"
                     /translation="MNQEVVATNLKVVEAHFHSEAANEIEKALELYTDDIIWESPTRD
                     LLFRGKEATAENYRKMFASFKVENFQTLQRFATETRVVDDSVVIAELIGDGVDNAPAP
                     VGSRVEIRLLHVFEMRDAKISRELVFENWRVIQPTILYSTTTVDLLHSSY"
     gene            2047..2835
                     /locus_tag="VFH91_01320"
     CDS             2047..2835
                     /locus_tag="VFH91_01320"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HET6861645.1"
                     /translation="MSTEIRTINVTRYVTPLREGGSLPAIIEADDDGLYVLKFRGAGQ
                     GLKALVAELLSGEIARHLGLRVPEIVFATLDHELSRLEPDPEIQDLLRASAGSNLAMD
                     FLPGSMTFDPIVEMVDGKLASQIVWLDALVTNVDRTARNTNILMWHKQLWLIDHGASL
                     FFHHAWKDWEEQSRRPFSQIKDHVLLRYATELEAVDREFRSQLTADVIGAIVGLIPDE
                     WLSDELPLRSVEEKRMVYKTFLKNRVEHSRVFVEEAQRAREVSV"
     gene            2813..3196
                     /locus_tag="VFH91_01325"
     CDS             2813..3196
                     /locus_tag="VFH91_01325"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HET6861646.1"
                     /translation="MQEKYLFEYAVIRLVPCVEREEFINVGVVLYCGRQEFLQMKFDL
                     NENRLKAFGCEVDISEIKDQLTGFERVCSGGTDAGTIGNLPMAARFRWLTAPRSTVVQ
                     TSPVHTGLTKDAAATLSELQAKLAS"
     gene            complement(3198..>3824)
                     /locus_tag="VFH91_01330"
     CDS             complement(3198..>3824)
                     /locus_tag="VFH91_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861647.1"
                     /translation="NTQDASKVIPRFFPKGYLSGYLLDHTRNWFVLLDPAAATAVKRE
                     ELQRFYEALLSNCYLGSRFLYAQRTSDDEEGISEQRANTPELVQFVRNHQYSKYKQGT
                     QSYDYLAERINSAERLHEYTDLLEGINQILRKEIAKLPPGKDLILREFPSDEEIIRRE
                     PSVMDCSKSCLGLPAGTKLYEVDVPAFHLQLAEIEGEMKIVSAMYSYQ"
BASE COUNT          968 a          933 c          948 g          975 t
ORIGIN      
        1 gaaccaacag cccaaccgtg gatcttgtca gcgctgaact ttattgcatt tagggaattg
       61 ccctggggca aaggattttg ccattgccag ccccgaaatg gatcctgagc cacacttaaa
      121 ggaactggga aaagtgaaga caaaaacagg caagctaccg cggcacggcc agctggacgc
      181 aacccggaat tgaggaccaa taggcggagc agactacgca ctttgtgaga cactacaacc
      241 aatcccagac cgggcatagg ggagaccgaa ttatagagat acaagggggc gaaagcgaac
      301 gtcgaagtat tacaaatttc ttgccaccgc ctaatagatc ctccaggttg aataagtcct
      361 atcacttacc cctgtcgcca gaacccaccg tgtcttcggc atgacgcagg atcgattcat
      421 cgacagttcc tgaggcggca attcgccggg cggcggtatc agcagcctcc tgcacgagat
      481 ggcccacgtg cccgcgcgtc caggcccatt caatgcggtg aggttttgct gcattgtcca
      541 aacgctccca ccaccgatga ttagccttac gacgaaaacg tccagacatc gtctcaatca
      601 cgtaacgaga gtccgaaaaa atcttcacgc gacagggttc gcgcagcttc tcgagtccga
      661 tcgcggcagc aacaatctct gcttgctgat tggtcgcgtt gccgatgtat tcgccaaccg
      721 ccttccaggt gcctttataa ccaagcaagg caactgcagc cgctcgcgcg acctcacgtc
      781 cgttgctgag actggaacca tcgcaaaaga tggttacctc ttttttgggg atgaccgggt
      841 tgtcttgatt gctactcaat caggtttcca ctttccagat gtcgtgagct ttaagtatgt
      901 cgtctgaata acacaaggcc tcttcaatac gctctgtgta gttttcaatc aagtactcga
      961 ggaaacttgc gacgaacttt cgctgttcga tagtacaagc agctactcga cgattatcct
     1021 tgccgtcgca aattaaatga aagagaaact gatcgagata ttgatttccg tctattgtat
     1081 ccagcgtcag tcggatcaag gccggcatgt agtaaaggaa tccatcggac gtacaaaagc
     1141 aaatcggatc ccagccggga tttcccaatt cagtaacacc aattgagtcc acgtcactgg
     1201 aaacaagcac ctgatcgtgt tcggcacatt cacagcaatg acgaaagtct gtgaagtgct
     1261 cgggcttcgg aacgttgaag atccgttttg cttcctgggt ccagttcatg aacaactaaa
     1321 tctatcgcga ttggttgccc tgaagaatga atcccgagaa ggcactctgt caatcgtcgc
     1381 aacacaagtc agtactgatt cgctaatatg aggaatggag cagatcaacg gtggtagtcg
     1441 aataaaggat ggtgggttga attactctcc agttttcaaa aactaactct cgtgagatct
     1501 ttgcatctcg catttcaaag acgtgcaaca gccttatctc aactcgcgaa ccaaccggag
     1561 ccggagcatt gtcaacacca tctccaatta gttcggcaat tactacgcta tcgtcgacca
     1621 cacgagtttc ggtcgcaaag cgttgtagtg tttgaaagtt ctcgactttg aacgaggcaa
     1681 acatcttacg atagttctcg gcggtagctt ctttacctcg gaaaaggaga tcgcgagtcg
     1741 gtgattccca gataatgtcg tctgtataaa gctcaagtgc tttctcgatc tcgtttgctg
     1801 cttcggaatg aaaatgggct tcaactactt tcaggtttgt tgctacaact tcttgattca
     1861 taactactta tctcccgctg gaccattgtc ctactcttat tcgctctatt tttttctcca
     1921 ttacgtaacg cgaattccgt ctcaggaatg ggccacacaa agccgaatgt atacaggcgg
     1981 acgggtctgc cgtaacatgt cgcgcgttga gatcacgact gtaatcaatg ccgcatgcca
     2041 taaagagtga gcacagaaat cagaacaatt aatgtgacgc gctatgtcac accgctgcgc
     2101 gaaggcgggt cactgccggc tattatcgaa gccgacgacg atggattgta tgtacttaag
     2161 ttccgcggag cagggcaagg cctgaaagcg ctagtggctg aactactctc aggtgagatc
     2221 gctcgtcatc tcggtttgcg cgtgcccgaa attgtattcg cgactcttga tcacgagtta
     2281 agccgtctgg aacctgatcc tgagattcaa gacttgcttc gcgcgagtgc cggttctaac
     2341 ctcgcgatgg atttcctgcc gggttcgatg acgtttgatc ctattgttga aatggttgac
     2401 ggcaaactcg cgtcgcaaat agtttggctc gatgcgcttg tcaccaatgt cgatcgaacg
     2461 gcgcgcaata cgaacatttt gatgtggcac aaacaattat ggttgatcga tcatggagcg
     2521 tccctctttt ttcaccatgc ctggaaagac tgggaagaac aaagccgcag accgttctca
     2581 cagataaagg accatgtctt gctgcgttat gcgactgaac ttgaagctgt cgatcgtgaa
     2641 tttagatctc aacttaccgc cgacgtgatt ggagcgatcg ttggtttgat tcctgatgaa
     2701 tggctgtcag atgagttgcc gctcagaagt gttgaagaaa agcgaatggt ctacaaaaca
     2761 ttcttgaaaa acagagtcga acactcgcga gtgtttgttg aggaggcgca gcgtgcaaga
     2821 gaagtatctg tttgaatacg ctgtaattcg cctggtgccg tgcgtcgagc gcgaagagtt
     2881 catcaacgtt ggtgtggtgc tctactgtgg tcggcaagag tttctgcaga tgaaatttga
     2941 tctcaatgag aatcgtttga aggcgtttgg ctgtgaggta gacatttcgg agattaaaga
     3001 tcagctgact ggcttcgagc gagtttgttc gggcgggacc gatgccggga cgattggaaa
     3061 cctgccgatg gccgctcgat tcagatggct aaccgctccg cgtagcacag ttgtacaaac
     3121 ttctccggta cataccggtt tgactaaaga tgctgcggca actctttcag aacttcaggc
     3181 aaaactcgcc agttagacta ttgataggag tacatggccg agactatctt catctctccc
     3241 tcgatctctg ccaactgtaa atgaaacgca ggcacgtcga cttcatacaa tttggtgccg
     3301 gccggtagtc ccaagcacga ctttgaacaa tccataacgg atggttcccg tcggataatt
     3361 tcttcgtcgc tggggaactc acgcaagatg agatccttac caggagggag tttagctatc
     3421 tcttttctga ggatttggtt gatgccttca aggaggtctg tgtactcgtg caaacgttcg
     3481 gcgctgttga ttctttcagc caggtagtca taagactgag taccttgttt gtacttgcta
     3541 tattgatggt tcctgacgaa ttggaccaac tcgggcgtat tagctctttg ttctgatatt
     3601 ccttcttcat catcagaggt tcgttgcgca taaagaaaac ggcttcccag ataacaatta
     3661 ctaagcaatg cctcatagaa ccgctggagt tcttcgcgtt tgacagccgt tgctgccgct
     3721 ggatccagca agacaaacca attcctggtg tggtctaaca gatagcctga caagtagccc
     3781 ttgggaaaaa aacgcggaat aactttgctg gcgtcttgag tatt
//
LOCUS       DASSFP010000065         6417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2098092, whole genome shotgun sequence.
ACCESSION   DASSFP010000065 DASSFP010000000
VERSION     DASSFP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098092"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFH91_01335"
     CDS             complement(<1..854)
                     /locus_tag="VFH91_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6861648.1"
                     /translation="MFRSSMARWILGLVVVISALAIVKFKPWRLMTKGDQQQTHARGD
                     LKVGFLPVTCHLTCPVTDYATRTSTMTHFESQRFTDFPTVVESIKSGRLEATFMIAPL
                     AMKLREQGVPVKICYLGHRDGSTVMVRKDLQAQSLRDLRGKTFAIPSKYSNQYLVIRK
                     LMDDQGVKPEEIKFVELPPPDMPGALAAKAIDAYFVGEPHAAKAELDGSGRVLYHAKD
                     IWPHFISCVLVVTEKLIREKPEVVRDLVRGIAESGEWAETHRIEAAKVVSPYFRQDEK
                     VVRYVLTQP"
     gene            complement(868..1608)
                     /locus_tag="VFH91_01340"
     CDS             complement(868..1608)
                     /locus_tag="VFH91_01340"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6861649.1"
                     /translation="MKDRPLVVDDVSVSFGAIAVFEHLSISIDEGEFVAVVGPSGCGK
                     TTLLNLLSQFLRPSAGKVRSAGRIRMVYQHDSLFPWQTVRENISLGLRELKNEAERNQ
                     QLADMLRLIKLEGFAEHYPHQLSGGMRQRVELARALIGNTDILLLDEPFSSLDYLARI
                     RMRNELARMLIQLPRTVVLVTHDIEEAAQLADRVIVLSDRPARVRSEFRVDLERPRQL
                     TEPIVVATVHQILSELGLEHERRQPVIA"
     gene            complement(1598..2374)
                     /locus_tag="VFH91_01345"
     CDS             complement(1598..2374)
                     /locus_tag="VFH91_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6861650.1"
                     /translation="MVIGKPVSSKERFLPILVLLVTVLIWMAVSAVHVFPESVFPSPL
                     DVAKGFMEELRAGRLLDDVVASLFRVTTGFALAVFTGVPAGLWLGHHARTRTAFLPLI
                     NFFRSLSPLAWIPFAILWFGVGDLPTVFLIFMASFFPLVLATVAAVENIPSVYFRIAS
                     DYGFNTFETLTRVTLPAIAPQVITALRVTAGLAWLVVVAAEMIAGRAGLGFAIWDARN
                     GLRMDLLVCGMIVIGIIGIVIDRILFQLTKIPSVRWGYER"
     gene            complement(2402..2626)
                     /locus_tag="VFH91_01350"
     CDS             complement(2402..2626)
                     /locus_tag="VFH91_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861651.1"
                     /translation="MSTDEILSNQQTIIANQQKIQANQEKLDRMLSNQEQIISNQKSI
                     LDNQLKLDSVVANQQRILANQEQILARLSK"
     gene            2956..>6417
                     /locus_tag="VFH91_01355"
     CDS             2956..>6417
                     /locus_tag="VFH91_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET6861652.1"
                     /translation="MNSLKSLRYPALVVCTLFLLALFSCRPESTLPKKSSKEYNEAVR
                     AFYVGLAALQVGHDIQADSKLAQMTQIVPEEPAGWANWGVLALRQRNYDAATERLEKA
                     RALAPDNDQINYLIGLLESSRGRQAEAIAALRKAVQLNPQNLIATYQLAQEIERQAGE
                     NTDPEFQQLISQILKVQPQNLAALLELARVSAKRGDAETLRNCLVTISNQSSSWPDEV
                     KQQLVAVQNAATGPDVTAAATRIVFFRNVLVRVPQYRQDLAQLKPPPGEEAVPFTHFV
                     KLETPSFNPAPPDTGISFKSEPLPEFSTKTWNWVGAVYFGIAGSPSTLVADAREVRTS
                     DGATMSFPAGASGAAPTIDGVLPIDFTYDFKTDLVLAGEGGVRLMRQDDPKHFTDVTN
                     AAKLPAGVTSAKYLGAWAADIEADGDLDIVLGTVEGNPTVLRNNGDGTFLEVHPFNNV
                     TGARGFAWADLDADGDPDAALIDASGKLHVFSNLRQGQFAERELPNLPAVKSINVADI
                     NDDGLLDLVAIEADGLIIRLSDKNEGAAWDSETLANLSDAKVPFGTEIRLRIADVDNN
                     GGLDLLVASPSEINKTLNCAIFLSDEKGHFNYLEQPPGVGRLFDAVDLNNDGRLDLIG
                     LAGDGEAMRALTETTKNYHWQVVRPHASQATGDQRINPFGVGGEMEIRAGLMLQKQPI
                     TGPVIHFGLGEQTGADVVRVVWPNGTVRAEFDVKGDQEIVTEQRLKASCPFLFAWNGK
                     QMEFVKDAVPWGSAIGLRINTLGSAKIAATGEWYKIRRDQLVPHDGYYDIRITAELWE
                     VYYYDYIALMPVDHPAGTEIFVDERFVVPPARLGLTTVGTPHKLAQAVDDNGQDVSEL
                     VGSLDGKAVDTFGRGQYQGLTRDHYLEVTLADDAPTNGPLYLVAHGSIHDTESSVNVA
                     ITQGTRWQAKSLSLEVPDGKGGWRPAQSNLGFPAGRKKTILFNLTGIFQPKTPRKVRI
                     RTNLEIYWDQIEWAQGLPDTQLKMDELKPSSVDLHYRGYSVIHRPDAGAPEIPDYNKL
                     SGSTQVWRDLIGYYTRYGEVGELLNKIDDRYVIVASGDEMSLRFPEQPPPPAGWVRDF
                     VIIGDGWIKDGDYNSTFSKTVLPLPYHAKQEYTAPPTILEEEDVYKRFPEDWQKYHTR
                     YITPEVFR"
BASE COUNT         1635 a         1693 c         1581 g         1508 t
ORIGIN      
        1 ggctgagtca aaacataacg aacaactttt tcatcctgac gaaaatatgg tgacacaact
       61 ttggccgctt cgatgcgatg cgtttcggcc cattctccac tctcagcgat gccacgaaca
      121 aggtctctca ccacttcagg cttttccctg atcagctttt cagtaacaac aagcacacat
      181 gaaataaaat gtggccagat atccttggcg tgataaagca ctcgcccact cccatccagc
      241 tcggccttcg ccgcatgagg ttcgcctaca aagtaagcgt caatcgcttt tgccgcgaga
      301 gcaccgggca tatcgggtgg cggcaactct acaaacttga tctcctcagg ctttacgccc
      361 tggtcatcca ttaacttccg aattacgaga tattgattgc tgtatttgct cggtatagcg
      421 aaagtctttc cgcgcaagtc acgaagactt tgcgcctgca gatctttccg caccataaca
      481 gtcgagccgt cgcgatggcc caggtagcaa atcttgactg gtacgccttg ttcgcgcagc
      541 ttcattgcga gcggcgcgat catgaacgtc gcttcaagac gaccgctctt gatggattca
      601 acaacggtcg gaaaatcggt gaagcgttgc gactcaaagt gagtcatggt gctggtgcgg
      661 gtcgcatagt cagtcacagg acaagtcaaa tgacatgtaa ctggcaagaa gccaaccttc
      721 aaatctcctc gcgcgtgagt ttgttgttgg tcacccttcg tcatcaagcg ccagggtttg
      781 aatttcacga tggccagagc cgatatcacc accaccaagc ctaatatcca acgagccata
      841 cttgaacgaa acataagtgc ctccgcttca cgctataacc ggctgccgcc tctcgtgctc
      901 gagccctaac tctgaaagaa tctgatgaac agtcgctaca actatcggct cggtaagttg
      961 ccggggacgt tcgagatcaa cgcgaaactc tgaacggacc cgagccggcc gatccgaaag
     1021 aacaatcact cggtccgcaa gctgagcagc ctcttcaata tcgtgcgtta ccaaaacgac
     1081 cgtacgagga agttggatca acatgcgggc aagctcgtta cgcatccgaa tgcgagccag
     1141 gtagtcgaga gacgaaaacg gctcgtctaa caaaagaata tctgtatttc caattagcgc
     1201 ccgagccagc tctacccttt gtctcattcc gcccgagagt tgatgtggat agtgttctgc
     1261 aaaaccctca agctttataa gacgaagcat atcggccagt tgctgatttc tttcggcctc
     1321 gttcttgagc tctctcagtc ccagcgaaat gttttcccga acagtctgcc acgggaatag
     1381 actgtcgtgc tggtagacca tgcgaattcg tccggcgctt cgcaccttgc cggcggacgg
     1441 ccgcaggaat tgcgataaca gattcagtag tgttgtctta ccgcaacctg atggaccaac
     1501 aacagccaca aactcacctt cgtcaatcga tattgagaga tgctcgaaga cggcgatggc
     1561 gccgaagctg acggaaacgt catcgacaac gagaggccta tctttcatag ccccacctta
     1621 cactcggaat ctttgtcagc tggaacaaga tgcgatcgat cacgattcct atgatcccaa
     1681 tcacgatcat tccgcaaact aataaatcca ttcgcaatcc gtttcgtgca tcccagatag
     1741 caaagcccag tccagcgcgc ccggcaatca tttccgccgc aactaccaca agccacgcca
     1801 atccagcagt cacccgcagc gcagttataa cctgtggggc tattgcggga agggtgactc
     1861 tggttaacgt ctcgaacgtg ttgaagccat aatcgctggc tattcgaaaa tagactgatg
     1921 gaatattttc aacggccgcc acggttgcga ggacgagcgg aaaaaacgaa gccatgaaga
     1981 tcaaaaacac cgtcggcaga tcaccaacgc caaaccatag aatcgcaaaa ggaatccacg
     2041 cgagtggtga taagctacgg aagaagttga tcaacggcag gaatgcggtt cgtgttctcg
     2101 catggtgtcc cagccagagc ccagccggaa cacctgtaaa tacggccagc gcaaagccgg
     2161 tggtaacgcg gaagagcgag gctacgacat catccaatag ccttcccgct cgcaactcct
     2221 ccatgaatcc tttagcaaca tctaagggcg atggaaaaac agattcagga aaaacatgga
     2281 cggccgaaac cgccatccag atcaacactg taaccagcag gaccagtatt ggcaaaaagc
     2341 gctctttgga cgaaaccggc tttccaatca ccatttctta cttcttagga ttgacgaaaa
     2401 attactttga taagcgcgcg agtatctgtt cctggttagc gagtattcgt tgctgattcg
     2461 ctacgacgct atccaacttg agctgattat caagaataga cttctgattc gagattatct
     2521 gctcctgatt ggagagcatc cgatccagct tttcctgatt tgcctggatc ttttgctgat
     2581 tggcaataat tgtttgctgg ttactcaaaa tctcgtctgt cgacatttcg catctcctgt
     2641 tatgaaattc ccgtagctat cctcgggttt gaaaatcatg aggtcttgtt ttcgtgatca
     2701 actttgaatg tccccgagtt accgggtggt gaatgcgacc aaccttcaat gtgcagtagc
     2761 aagccggtga actttaaatt ccgatcatgc ctgctcttat ggatcatgaa atccaccttt
     2821 agtttgagat cgaactcaaa ccgaaggtct agagattcgc ccaatcctgt atgctacatt
     2881 gtcgaacaca tttgatcgca gtgtcggtta acttctgctg gtcagtcctc ctgataagtt
     2941 aggcagaaaa tttgcatgaa ctcgcttaaa agtctcagat atcctgcact tgttgtgtgc
     3001 acgctcttct tacttgcact cttctcgtgc cgtcccgaga gcaccctgcc aaaaaaatct
     3061 tccaaagaat ataacgaggc agtgcgagcg ttttacgtag ggctcgccgc tttacaggtc
     3121 ggtcatgaca ttcaggctga tagtaagttg gctcaaatga cgcaaatcgt tccggaagag
     3181 cctgccggat gggccaactg gggagtcctc gctttgcgac agcgaaacta tgacgctgcg
     3241 accgaacgac tcgaaaaggc acgagctctt gctccagaca acgaccaaat caattactta
     3301 atcggactac ttgagagtag tcgaggtagg caagctgagg caatcgccgc tctgcgtaaa
     3361 gcggtccaac tcaatccaca gaatttgatt gcgacctatc aactcgccca ggagattgag
     3421 cgtcaagccg gagagaatac cgaccctgaa tttcaacaac tgatttcgca gatcctgaag
     3481 gtgcagccgc aaaatctcgc agcccttctg gagttggcac gcgtttcggc aaaacgcggt
     3541 gacgccgaaa cactgagaaa ctgtctcgtc actatctcta atcaatcatc gtcatggcca
     3601 gatgaagtga aacagcaatt agttgccgtc caaaatgccg caactggtcc tgacgtaacg
     3661 gccgctgcaa ctcgaattgt cttctttcgc aatgtgctcg tgcgtgttcc tcagtatcga
     3721 caggacttgg cccaactaaa accaccacca ggcgaagaag cggttccttt tacgcacttc
     3781 gtcaaactcg agactcccag tttcaacccc gctcctcctg atactggtat ctcattcaag
     3841 agtgaacctc tcccggagtt ctccacaaaa acatggaatt gggttggcgc agtctatttt
     3901 ggaatcgccg gatctccatc taccttggtc gccgacgcac gcgaggttcg cacaagtgat
     3961 ggcgccacga tgtcattccc ggcgggtgca agtggcgctg cgccaacaat agatggagtt
     4021 cttccgatag actttaccta cgacttcaaa accgatctcg ttttagctgg cgaaggtgga
     4081 gttcgcctga tgcgtcagga tgatcccaaa cacttcacgg acgtgacgaa cgcagccaaa
     4141 ttgccggccg gtgtaacctc agcaaaatat ctgggtgcct gggccgctga cattgaggct
     4201 gatggagatc tcgatatcgt actcggcacg gtggaaggta acccgactgt cttaagaaac
     4261 aatggtgacg gcacattttt agaagtccat cctttcaaca atgtaaccgg tgctcgcggt
     4321 tttgcctggg ctgaccttga cgcggatggc gatccagatg ccgctttaat cgatgcgtcc
     4381 ggaaaacttc atgtgttctc gaatttacga caaggtcagt ttgcagagcg tgaactgcca
     4441 aacctaccgg ccgttaagtc gatcaatgtt gcggacatta acgacgacgg cttgttagat
     4501 ctggtcgcaa tcgaggctga tggtctaatt attcgacttt cagacaagaa cgaaggtgct
     4561 gcctgggaca gtgagacatt ggccaatctt agtgacgcaa aagtgccttt cggaactgag
     4621 attcgcttgc gaattgctga cgtggacaat aatggcggtc ttgatctttt agtcgcatca
     4681 ccctccgaaa taaacaagac cctgaactgc gcgatatttc tgagcgacga gaaaggccat
     4741 ttcaattacc tcgaacagcc tccgggtgtt ggtcgcttat tcgacgccgt tgatttaaac
     4801 aacgacggtc gtcttgattt aatcggactg gcgggtgacg gtgaagcaat gcgagccctc
     4861 actgaaacca ccaaaaacta tcactggcaa gttgtccgac cgcacgccag tcaggcaact
     4921 ggagatcagc gcatcaaccc gtttggcgtt ggcggggaaa tggaaatacg cgccggactc
     4981 atgctgcaaa agcaaccaat caccggtcct gtgattcatt ttggattagg cgaacagact
     5041 ggagctgatg ttgttcgagt cgtatggccc aatggaacgg tgcgcgctga gtttgatgta
     5101 aaaggcgacc aggagatcgt tacagagcag cgcttaaaag cgtcgtgtcc cttcctattt
     5161 gcctggaatg ggaaacagat ggagttcgtg aaggacgccg taccatgggg atcggcgatt
     5221 ggtctgcgga ttaacacgct tggctcagca aagattgccg cgactggaga gtggtacaaa
     5281 atacgtcgcg atcagttggt gccccacgac ggctattacg acattcgcat cactgctgaa
     5341 ctttgggaag tctactatta cgactacatc gcgttaatgc cggtggacca tccggccggc
     5401 accgagattt tcgttgatga gcgatttgtg gttccgccgg ccaggttagg cctgactaca
     5461 gttggcactc cccataagct ggcccaggca gtcgacgata atggccagga tgtgagcgaa
     5521 ctagtgggct cacttgatgg taaagccgtt gatactttcg gtcgcggaca ataccaggga
     5581 ctcacacgcg atcattacct ggaagtcact cttgctgatg acgctccgac taatggccca
     5641 ctttatctgg ttgcgcacgg ctcaatccac gataccgagt cgtcagtaaa cgttgcgatt
     5701 acgcagggca ctcgctggca ggcgaagagc ttgagtttgg aagttcctga cggcaagggc
     5761 ggttggagac ctgctcagtc caatctcggt tttcctgcag gtcgtaagaa gactattctt
     5821 ttcaatctga ctggcatttt ccagccgaaa actccacgga aagttcgtat cagaacgaac
     5881 cttgaaatct actgggacca gattgaatgg gctcaggggt tgccggatac ccaactcaag
     5941 atggacgagt taaaaccatc aagcgtcgat cttcattatc gaggttactc ggtaattcat
     6001 cgtcctgatg cgggtgctcc ggagattccg gattacaaca agctctccgg ctcgacccag
     6061 gtctggcgcg acctgatcgg ttattacacg cgctatggcg aagttggaga gttgctaaac
     6121 aagatcgatg atcgctacgt catagtcgca tcaggtgatg agatgtcgct acgctttcca
     6181 gagcagccgc caccgcccgc gggttgggtc cgtgacttcg taatcatagg tgacggttgg
     6241 atcaaagacg gcgattacaa ttcgaccttc tcaaaaaccg ttctgccttt gccgtatcac
     6301 gccaaacagg aatacactgc gcctcccacg attcttgaag aagaagacgt ttacaagcgc
     6361 tttccggaag actggcagaa ataccacacg cgttacatca cacccgaagt atttcgc
//
LOCUS       DASSFP010000066         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1952361, whole genome shotgun sequence.
ACCESSION   DASSFP010000066 DASSFP010000000
VERSION     DASSFP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952361"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFH91_01360"
     CDS             <1..989
                     /locus_tag="VFH91_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861653.1"
                     /translation="DSGYASFRVVMSDTRLANSFTSGMYGGPLPFEPTVTVETNGIIR
                     RLLVSKENGKGSVIFGYELEIVPLPNTRQFSVIARPLDPAFASTLHGRGESTPTLVSA
                     TGPRLISDRETFALDLLVNEQRRIKFVDYVRVALDKEGISPGGVLQPPRDFTLANVEL
                     SIKNYLLVVNGEVVPTLSVRHDCTGSLIWFSDPEGGRFIFSLVPYPGMTFQKVGVIED
                     NRISFSWKNSKYEWISTEPIVGTRGRWNLWVLHDRNYGQFYPVPSLERPKNKNNNSTA
                     QDLWTNPMGIIKRESTPHVRTLQKQSEASPAPPPIRIQIGGATTIKELLPDN"
     gene            1020..1694
                     /locus_tag="VFH91_01365"
     CDS             1020..1694
                     /locus_tag="VFH91_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861654.1"
                     /translation="MKERIVLTVVVCLVSLAYDLPCKGQADDELKRFEAAVLFSSITK
                     PEFSGTTADAGFGGRLTFNLNRSIALETEGIFFPSRCTGCRPENRGRLTQFFAGVKAG
                     KRFDQFGIFFKARPGLASFSDGGNRITFIPIAAGQFPFIPLILFDSQRKTNFATDLGV
                     AVEIYHSKRIFTRFDAGDTIIRYSDRQAIFFNLDPAGNVFPVQVKLPNKTLHNFQFSA
                     SVGFRF"
     gene            2028..3689
                     /locus_tag="VFH91_01370"
     CDS             2028..3689
                     /locus_tag="VFH91_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6861655.1"
                     /translation="MSNMQANIKSVVHLMLENRSFDSLLGWLRWDGQARPKNHVPALR
                     PNEKEFYGLQGTEWLPSNYSYFLSHGPFTYKREPITKITPGSSWDYCEMPASDPGEEF
                     EHVTAQLKAASYAPSIPMGYYLDYLSVYYDGSARDILGVFVPETLPCLSTLAARFAVS
                     DMWFSSVPTQTNPNRAFSLCGTSLGRINNNDDLNTYRFCGQPYGNINNIFSVFNAFNT
                     ANPGLPPVTWKLYSRWNWHDVARDPTHPFETGWNSWQAGGEAGKLCFNATKGKYFTPY
                     MFPKGFDNKQWGGIGSIDDFKRDVAQNTLPAFSYIEPAFFPQTLDLNDLTDYHPPESV
                     YAGEGFVKDIFNTITSNPKVWANTLFIITFDEHGGTYDHLMPPTNAVPPDTNTNPSFD
                     FKRYGVRIPTLLISPWVTPGTIFRAGYYPEVADKSLLPFDHTSVLATLCKWKGINYRS
                     KAQFPNYYLGDRTAAAPTFDSVITNTSNAAKPTLAQHSCGAGLTGIVKRRELIRSMIR
                     GVAKITGRSEAEVAAQGTVEEIHKTCKSAAELEQYFKDLAAKHGK"
BASE COUNT          996 a          971 c          937 g          961 t
ORIGIN      
        1 aagacagtgg ctatgcgagc tttcgagtgg tgatgtctga tacccgctta gccaattcct
       61 tcacatccgg tatgtacggt gggccgttgc cattcgaacc gaccgtaacg gtcgagacta
      121 acggcatcat ccgacgcctc ctcgtaagta aagagaatgg gaagggaagt gtcatttttg
      181 gttatgagct ggagatcgtg ccgttgccga acactcgcca attcagcgtc attgcgcgac
      241 ctcttgaccc cgcatttgct tcaacgctcc atggacgcgg cgaaagtact ccaacccttg
      301 tttctgcaac tggtccaagg ttgattagcg atcgagagac attcgcgctg gatctgctgg
      361 tgaacgaaca gcgccggatc aagttcgtcg actatgttcg tgtcgcactg gataaagagg
      421 gcatctcacc tggaggagtt ttgcaaccgc ctcgtgactt tactcttgca aacgttgaac
      481 tctcgatcaa gaactacctc cttgttgtca atggtgaagt tgtcccgacg ctgagcgtgc
      541 gtcatgactg tacaggatct ttgatatggt tttcagaccc tgaaggaggt cgtttcattt
      601 tttcgcttgt tccttatccc ggaatgactt tccagaaagt tggagtgatt gaggacaatc
      661 gaatctcatt tagctggaag aatagtaaat atgagtggat ttcaactgag cctatcgtag
      721 ggactcgcgg ccgctggaac ttatgggttc tgcacgatcg gaattatggt cagttttatc
      781 cagtaccttc tttagaacgt cctaagaata agaataataa tagtaccgcc caggacctat
      841 ggaccaaccc gatgggaatc atcaagcgag aaagtactcc ccacgtgcgg acgttacaga
      901 aacagagtga ggcctcaccg gcaccgcccc caataagaat tcaaattggt ggggcgacaa
      961 ctataaaaga gctactcccc gataactaag acctactaag aaaaaggatt aggtgaacaa
     1021 tgaaggaacg catagttctg acggtcgtgg tgtgtcttgt ttcgctcgcg tacgatttgc
     1081 cttgcaaggg ccaagcagac gacgagctga aaagattcga agcagccgtg ctcttcagtt
     1141 caattacaaa acctgaattc agcggcacca ccgctgatgc tggtttcggt ggacgcctaa
     1201 ctttcaactt gaacaggagt atcgctcttg agactgaggg gatcttcttt ccctcacgat
     1261 gcacagggtg tcgtccggag aacagaggtc gcctcaccca atttttcgct ggagttaaag
     1321 ccggtaagcg cttcgatcag tttggaatat tctttaaagc caggccgggc ctggcaagtt
     1381 tcagcgatgg cggaaatcgt ataacgttca ttcctatcgc agcgggccag ttcccattta
     1441 tcccacttat cctttttgat tctcaacgca aaaccaattt tgcgaccgat cttggagtgg
     1501 cagtcgagat ttaccattca aagagaatct tcacgaggtt tgatgctgga gacacgatca
     1561 tcaggtatag cgacaggcag gcgattttct ttaacttaga tcctgccggc aacgtatttc
     1621 cggttcaagt aaagctaccc aacaagacgc tacacaattt tcaatttagc gcgagtgtcg
     1681 gctttcggtt ttgataatga tcgctcgtct aaccatccac agattgcgca gatgacgccg
     1741 atttctaaga acacagagag tggtgtctac accattgagc ttagagatcg gcgtcatcta
     1801 acttggcttt ctggtccaaa tgaagctact agtagcctga caaattcttt ttgatctgcg
     1861 acaatgtcgt ctcgcaaaag taaccacctc gctcacaaat aacgccctga aagtcgctca
     1921 cttactctga gtggcgcact tgtgagtttt agcgaccttt acgatactcc tacaaatatg
     1981 aacgaaccaa tttgatggtt ctcataccac tctgaaagga caacctcatg tcgaacatgc
     2041 aagccaatat caaatccgtg gtccatttaa tgctcgagaa ccgcagtttt gacagcctgc
     2101 tcggttggct gcgatgggac ggtcaagctc gccctaagaa ccacgttcct gcgttacggc
     2161 cgaacgaaaa agagttttac ggcttacagg gaaccgaatg gttaccgtcg aactacagct
     2221 attttctttc tcacggtcct tttacttaca aacgagaacc aataacaaag atcactcctg
     2281 gcagctcctg ggattattgt gaaatgccgg cgagcgatcc cggcgaggag ttcgagcatg
     2341 taacggcgca actgaaggcc gcatcatatg ctccttcaat accgatgggt tattacctcg
     2401 attatctgag tgtttactac gacggaagcg caagggatat tctcggagtc tttgtgccag
     2461 agactttgcc gtgtctcagt acgctcgccg ccagatttgc ggtttcagat atgtggtttt
     2521 catccgtgcc aacccaaacc aatcctaacc gagctttcag tttatgtggc acctcattgg
     2581 gccgaatcaa taataatgac gacctgaaca cttatagatt ttgtggacag ccttacggca
     2641 acatcaacaa cattttttcc gtcttcaatg cattcaatac ggccaatcca ggtctaccgc
     2701 ctgtcacctg gaaactctac tcgcgctgga attggcacga cgtcgcccgc gacccgaccc
     2761 atcctttcga aactggatgg aattcatggc aagctggcgg cgaagccggc aaattgtgtt
     2821 tcaatgcaac caaaggcaag tactttacgc cttacatgtt tccgaaaggc tttgataata
     2881 agcagtgggg cgggataggg agcatcgacg attttaagag agatgttgcg caaaatacgc
     2941 tgcctgcctt tagttatatt gagccggcat ttttccctca aactctcgat ctgaacgacc
     3001 tgacagacta ccacccgcca gagagcgtct atgcgggcga aggctttgta aaggatatct
     3061 tcaacacgat taccagcaac ccgaaagttt gggccaacac actctttata attacgttcg
     3121 acgagcatgg cgggacctat gaccatctaa tgccaccgac caacgcagtt ccacctgaca
     3181 ccaatacgaa ccccagcttc gatttcaagc gctatggtgt gaggattccg acgcttttga
     3241 tttcgccatg ggtgactcca ggaacgattt tccgagctgg atactatccg gaggttgcag
     3301 ataaatcgct tttgccgttt gaccacacct cagtcctggc aacactctgt aagtggaagg
     3361 ggatcaatta caggagcaag gcccaattcc caaactatta tctgggcgat cgcaccgccg
     3421 ccgctcccac ttttgacagc gtgattacga acaccagcaa cgcggccaag ccgaccctgg
     3481 ctcagcacag ttgcggcgca gggctgacgg gaatcgtcaa acgtcgtgaa ttgatcagat
     3541 cgatgatccg aggggtggcc aaaatcaccg gccggtctga agctgaggtc gcggcgcaag
     3601 gaacggttga agagattcat aaaacctgta agagcgcagc agaactggag cagtacttta
     3661 aggaccttgc agccaagcat ggcaagtagc tgaaccgcac gccattaacc tgagccagca
     3721 actacataat atgtattatc agacgcaagc gattagcgag agaatgctga ggttttcgta
     3781 gcttaacctg ctctgcttaa gcttaaggcg gcttctttgg actccgagcg gtcggcgatt
     3841 tcgttaaggg gctgatgatc aggct
//
LOCUS       DASSFP010000067        12489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1208322, whole genome shotgun sequence.
ACCESSION   DASSFP010000067 DASSFP010000000
VERSION     DASSFP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208322"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..7366
                     /locus_tag="VFH91_01375"
     CDS             11..7366
                     /locus_tag="VFH91_01375"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET6861656.1"
                     /translation="MVVPTIFSSESQVSELIERLEVHFLANQEKQIYFGILGDFPDAA
                     SETIATDDRLLAVAQSGIEELNRKHGGGEYQRFNLFHRRRLWNEGEKKWMGWERKRGK
                     LEEFNSLLRGNRQTTFIVNTADDSLLASTRYVITLDSDTQLPRDVARTLIGTAIHPLN
                     RPYIDPSINRVTRGYGIIQPRVSISLSSASRSRFAEVFSGNTGIDPYTTAVSDVYQDL
                     FGEGSFTGKGLYDVDAFQTTLSDRVPENALLSHDLFESLFARAALTTDIELLDDYPSS
                     YEAYSKRLHRWTRGDWQILSWILPSVKDSSGNKVRNTLPVIARWKILDNIRRSLVAPS
                     LFIWLTAACAFFPGRPLLWSMFVLLNIAFPVYLHVSTGLLIHPRGIPWTSHFWTVWGD
                     IRTNTFQIVLSFLFLPHQAYLMADAVVRTLYRKLISRKNLLEWVSAAEAERSARQDFL
                     GFLSYMLPAVLLTLAAALLTFLLKPSAMPVMSGLAVLWLFSPLIAYLVSRNISRELQS
                     FTAEEIALIRVIARRTWRFFETFVGAQDNWLPPDNFQEDPNPVIAHRTSPTNIGLLLL
                     ATASARDLGYVGCLEFIERQELTLSTVSSLKKLHGHLFNWYDTTTLEPLLPQYISTVD
                     SGNLAGHLHAVKQACVELPDLRLFSKKVVEGLTDTIDAISVEVTRLGEIRQRTEVVTV
                     SQLRDEVAACRMLAMMDTHENLASWFVLVDSLSRRASEIEDIVNALAHEHGEVNFKEL
                     RWWVGALQHQVQSCQRDGDTLAAWGRLLALLESEGTAASEEWKSILEQLKVVPTLAEV
                     PVICDRALVQIAVLQSNDGSTSHQSATRLTRALEQAAAASNDFLSRLSRLARTCDQII
                     ENMNFGFLFDVERKLFPIGYSVGTAKADDSFYDLLASEARLASFVAIAKGDVPQEHWF
                     RMGRPLAKVDSGRALISWTGTMFEYLMPLLVMRNYPGTLLDETYRTVVHRQIEYGVER
                     NVPWGISEAAYNVRDLNFNYQYGPFGVPGLGLKRGLIEDLVVAPYSCILAIEIAPHAT
                     VNNLRRLQSRGALGPYGFYESIDFTGERVPEGQTSVLIRAFMTHHQGMSLVALANILQ
                     EDPTEKRFHAEPVVQATELLLQERIPVGVPAVHPRAEEVLTGRVTQALPGMITRIYNS
                     PHLDTPRTQFLSNGRYNVMVTTAGGGYSQCDGNAVTRWREDVTRDNWGAFIYLRDVRS
                     GTIWSPGYQPTQKKPLSYHVAFSEDKVEVRRTDPGIATRMEILVSAEDNAEIRRISLT
                     NTSTRVREIELTSYSEIVLASPQADAAHPAFSNLFIETECIVNENALIAHRRRRSSDE
                     QQIWGIHVVVIEGELIGALQYETDRGRFLGRGRSTNNPIAVMEDRPLSNTTGAVLDPV
                     FSLRRRVRIQPNETVTCSFTTGVAFSREEALALADKYHDPNIFERELRLSWTKAQVEM
                     SHLSIDSEEAHLFQRLAARIVYSDPSLRPQPHVLALNTKAQSSLWAYGISGDLPIVVV
                     RIDSEGDLGTVRKLVRGHEYLHYKGLRVDLIVLNDSPTNYQQQLHHELETIIRTSGLQ
                     GLQDKPGGVYLRRSDQITEPDRILIHAVARVVIVADRGSVQDQIERPRVEEPLPAPLV
                     ARLASQTYPEAPVVRPDLTFFNGLGGFYQGGREYVTVLGAGQWTPAPWSNVIGNEDEF
                     GFQVTETGAGYTWSVNSRENRLTPWSNDAVSDPPGEAVYIRDEDSGTIWTPTPLPVRE
                     NEPYTVRHGQGYTVFEHTSHGISQELLVFTPLDASVKISLLRLRNRTPRTRKLSITNY
                     NELVLGVARSSSAPYIITEIDEAAASIFARNPFNNEFSTRIAFNATNEPLASATCDRK
                     EFIGRNGSLDQPAGLRRVSLANRDGAGLDPCAALQIQLELAPHEGREVIFLLGETETK
                     EKCQELIEKFKQPGNVNAAFEKVLSHWDQLLGAVEVRTPDAALDTMLNRWLLYQTLSC
                     RVLARTAFYQSGGAFGFRDQLQDVMALVYSAPEVARKQILLAASHQFKEGDVQHWWHP
                     PTGRGVRTRISDDLLWLPFVTAFYIKVTADTSVLDEVVGFLEQPILHEDEQEAYLQPS
                     IAEETATIYEHCARALDRSLAVGPHGLPLMGAGDWNDGMNRVGQHGKGESVWLGWFLF
                     TTLNGFLPHCEMRQESTRVEAYEKHCDNLKKALEEKGWDGDWYRRAYFDDGTPLGSSQ
                     NEECRIDSIAQSWSVISGASDQYRMSRAMAAVEEYLIRRGDGLVILFTPPFDKGSLDP
                     GYIKGYVPGVRENGGQYTHAAIWTMIAFSMLGDGEKAGELFSLLNPINHASTRAGLHK
                     YKVEPYVAVGDVYAVPPHTGRGGWTWYTGSAGWMYRAGLESILGFKLQGDRLLIDPAV
                     PRWWRDFEITYRRKNGVYRIKVENPSGFSRGVASVELDGIVLEKDEIPLTGDGQTHNV
                     RVVMGERVRDTTPEAAEEHAGETGTSKRG"
     gene            complement(7412..8155)
                     /locus_tag="VFH91_01380"
     CDS             complement(7412..8155)
                     /locus_tag="VFH91_01380"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6861657.1"
                     /translation="MSGHSKWHSIKHKKGALDAKRGKLFTKFIKEITVSARTGGGDPD
                     ANARLRKAILDAKSGNMPNDTIERAIRRGTGEEEGVNYEEITYEGYGPGGVALLIESM
                     TDNRNRTVAEIRHIFSKNGGNLGESGSVGWMFEKKGYIVVDKAAKTEDELFELAIEAG
                     ADDLRDDEDNFEIITSPEAFDGVLTAVKGAGIEPQVAEVEMVPQNYIRLEGADARQML
                     KLMEALEDHDDVQKVSANFDISEADMAAA"
     gene            complement(8332..11253)
                     /locus_tag="VFH91_01385"
     CDS             complement(8332..11253)
                     /locus_tag="VFH91_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6861658.1"
                     /translation="MDSDKVSHYRILEKLGAGGMGEVYLAEDLKLGRKVAIKILSEEY
                     TTNRDRLSRFELEASAASALNHPNILTIHEVGVDEGRHYIATEYIDGVTLRRKMAASH
                     LEIREVLDIAVQIASALEEAHAAGIVHRDIKPDNIMIRRNGFVKVLDFGLAKLTEGTT
                     DRLPSDGEASTRVLVQTDAGVVMGTSHYMSPEQARGKQVDARSDIWSLGVVIYEMIAG
                     RTPFEGETPTDVIVGITQKEPPPLARFAPDAPAELDWIVTKALRKDREERYQTIKEVL
                     TDLRRLKQRVEFEIEAERSVPPDSRTRSLASGKVAPTFQDRALTTAEKTVSQVSSAEY
                     IVGGIKRHKIAALLALVIVAAIASGSYFYFKRKPVLTNSDTILLTEFINTTGEQVFDG
                     TLKQALSVQLGQTPFLNIFPDDRVRETLRFMGRSADERITKEVGKEICERQGIKAMLV
                     GSIASLGSHYVITLEALNARSGDPIAREQIEAESKEKVLSSLGTAASSLRQKLGESLS
                     SVKQYDVAIEQATTSSLEALKAYAMGNEERNKGKVREALAFYQRAVDLDPNFAMAYAR
                     IAVFYGNQSQFESAKGYVQKAYDLRDRVSEREKLYITEKYYSYVTGEIDKAIETQQTW
                     AKLYPTDFIPHNNLALNYLYFGRYDDALKEALEAVRLGPNNVSARENLIGSFMGLERM
                     DEAEQAANELLKINPEYLGGHYHSYVFAFMRGDQARMKQETEWARGKLDEPELIANLA
                     WAETFSGRLKKGEELAKQSSDMFLSKGSKENAANTLVMLSQTESVVGKCQAAKENVQR
                     ALTIFKGRFSMSSGAITLAACGDVSKSQTLLDEAVKEYPKDLPVTMISGPLTKALIEK
                     DRGNLDQATQYLESIRRYDLGIIAGLANNYARGLVLLDQKKGTEATVEFKKILEHRAI
                     DFFSPAHALAQLQLARAAATSGDVASSRKYYQDFFAAWRDADQDLPVLVEAKKGYAQL
                     Q"
     gene            complement(11450..11758)
                     /locus_tag="VFH91_01390"
     CDS             complement(11450..11758)
                     /locus_tag="VFH91_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET6861659.1"
                     /translation="MIEHTYANDSLVLKVRVVPRASRSEVVGEFDRSLRVRVASAPVD
                     GAANDELIQVLARTFKVPRVAVRILRGVSSRTKQVQVDGPDFDAMRNLGILRKKEQTA
                     "
     gene            complement(11769..12335)
                     /locus_tag="VFH91_01395"
     CDS             complement(11769..12335)
                     /locus_tag="VFH91_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET6861660.1"
                     /translation="MAVTAIIIVIIALMILRLIANASNLNPFAWASRTIHRLTDVFVL
                     PMRRGLMSLGFDPKYAPLVVILVTIVLGLFVNQLAGKLGSTALGVLDGIQDGRPVLVV
                     GHILNGAISVYILFITMWIIFSWVRVSYSNRIMRFLVTVTEPLLGPLRRLIPPLGMFD
                     ISPLVALLALWLIQGAIWGTLLSDGRLR"
BASE COUNT         3019 a         3265 c         3147 g         3058 t
ORIGIN      
        1 caggactttt gtggttgttc caacgatctt ctcatcggaa tctcaagtat cagaactcat
       61 tgaacgactc gaagtccatt ttctggcgaa ccaggagaaa caaatttatt ttggaatact
      121 tggggatttt ccagatgctg cgtctgaaac gattgcaacc gatgatcgct tactcgcagt
      181 ggctcaaagc ggcatagaag agctcaacag gaagcacggc ggcggggagt atcaaaggtt
      241 taatctattt catcgtcgtc ggttgtggaa cgagggtgaa aaaaagtgga tgggatggga
      301 gcgcaagcgt ggaaaactcg aagagttcaa tagtcttctg cgcggaaatc gccaaacgac
      361 tttcattgtc aacacggctg atgattcgct gctggcgtca acgagatatg tcataactct
      421 cgactctgat acgcagctac ctcgggatgt cgctcgaaca cttattggta ccgcaattca
      481 tccactcaat aggccttata tcgatccgtc aataaacagg gtgacccgtg gatacggaat
      541 tattcaaccg cgggtgagta tttcgctgag cagcgcatcg cgttcgagat ttgcggaagt
      601 cttttctgga aataccggaa tcgatccata tacgaccgcc gtttcagatg tttatcagga
      661 cctctttggc gaaggtagtt tcaccggtaa aggtttgtac gacgtcgacg cgttccaaac
      721 aacgctaagc gatcgagtac ccgagaatgc tttattaagt catgacttat tcgagtcgtt
      781 gtttgcacgt gcggccctga caactgatat cgagctactc gatgattacc catcctccta
      841 cgaagcttac tcaaagagac tgcatcgctg gacacgtggc gactggcaaa tcctcagttg
      901 gattcttccc agtgttaagg attccagcgg caacaaagta cgtaacactc tgccggtcat
      961 tgcccgctgg aaaattctcg acaacatcag gcgtagtctg gttgcaccat cgttattcat
     1021 atggctgaca gctgcttgtg cgttctttcc cggtagacca cttttatgga gcatgtttgt
     1081 tcttctcaac atcgcctttc cggtgtacct gcacgtgagc accggattat taatccatcc
     1141 aagaggaata ccctggacca gccatttctg gaccgtgtgg ggtgacatcc gcaccaacac
     1201 gttccaaatt gttcttagct ttctctttct gccgcaccag gcgtacttga tggctgatgc
     1261 agtcgtccgg acgctgtatc gaaaacttat ctcacgcaag aacttgctcg agtgggtttc
     1321 tgcggctgag gctgagagat ccgccagaca agattttctc gggttcttat cgtacatgtt
     1381 gccagccgtc ctgttgactc tggcggcagc gttgctcacg ttcctgctaa agccatccgc
     1441 catgccggtc atgagtggat tggccgtcct ctggctgttt tcacctttga ttgcttatct
     1501 ggtgagccga aatatctccc gcgaacttca aagcttcacc gccgaagaga tagctttgat
     1561 ccgtgtcata gcacgtcgta catggagatt ttttgaaacg ttcgtgggcg ctcaagacaa
     1621 ttggcttcca cccgataact ttcaggaaga tccaaatcct gtcattgctc atagaacgtc
     1681 gccgaccaat ataggtcttt tgttactggc aacggcttca gcacgagatc tcggatatgt
     1741 gggctgtctg gagtttatag agcggcagga acttacgctc tcaacagtgt ccagcttaaa
     1801 gaagctccat ggtcaccttt tcaattggta cgacaccact acgctcgagc ctttattgcc
     1861 gcaatacatt tcgaccgttg acagcgggaa cctcgcaggc catctccatg cagtaaagca
     1921 ggcttgcgta gaattgccgg atttgaggct cttcagcaag aaagtagttg aaggactcac
     1981 tgacacgatc gacgcgatct cagttgaagt aaccaggctg ggcgagatca gacagcgcac
     2041 cgaagtcgtg actgtcagcc agttacggga tgaagtggcc gcctgtcgca tgctggcgat
     2101 gatggacaca cacgagaacc ttgcttcatg gtttgtactc gtcgattcct tgagccgccg
     2161 tgcttccgaa attgaagata tcgtgaatgc cctcgcccac gaacatggcg aagttaactt
     2221 caaagaatta cgttggtggg tcggcgcatt gcaacatcag gtccaatctt gccagcggga
     2281 tggcgatacc ctcgcagcat ggggacgcct gttggccctg cttgagtctg aaggaacggc
     2341 cgcatcggaa gaatggaaat cgatactgga acagctgaag gttgttccca cactcgccga
     2401 ggtaccagtg atttgcgatc gcgcattggt ccaaatagca gttctgcaaa gcaacgacgg
     2461 ttcaacttcc catcaatcgg ctacaagact aacgagagca cttgaacaag ccgcagccgc
     2521 ttccaacgat ttcctctcac gtttaagccg tcttgctcgg acctgcgatc aaatcatcga
     2581 gaacatgaat tttggcttcc tgttcgacgt tgagcgaaaa ctttttccga tcggttacag
     2641 cgtaggtaca gccaaagctg acgattcctt ctacgatctc ctggcctctg aagctcgact
     2701 ggccagtttc gttgcaattg ctaaaggtga tgtgccgcaa gagcactggt tccgtatggg
     2761 ccggccactg gcgaaagtcg acagcggtcg cgctttaatc tcctggaccg gaacgatgtt
     2821 cgagtacctc atgccgttac tggtgatgag gaattacccc ggcaccctgc tcgacgaaac
     2881 ctatcgaacg gttgttcatc gtcaaataga atacggtgtt gaacgtaatg ttccatgggg
     2941 aatatcggag gcggcctaca acgtcaggga tctcaacttc aactatcaat atggaccgtt
     3001 cggcgtcccc ggtcttggat tgaaacgagg gttaattgaa gatcttgtag tcgcaccgta
     3061 cagctgcatt ctggcgattg agatcgcgcc ccatgcgact gtcaataatc ttcgtcgctt
     3121 gcaaagcagg ggagcacttg ggccgtatgg cttctacgag tcaattgact tcaccggtga
     3181 gcgcgttcca gagggtcaaa cttcagttct gattcgggct ttcatgactc accatcaggg
     3241 catgagcctg gtggcccttg caaacatatt gcaggaagat ccaaccgaga aacgatttca
     3301 cgcagaacct gtggtccagg cgaccgagtt gctccttcag gaacgcatcc cggtgggagt
     3361 gccagccgta catcctcgag cagaggaagt gctcaccggt cgagtgacgc aggcgctgcc
     3421 ggggatgatc acgcgtattt ataattcacc acacctcgat acacctcgta cccagttcct
     3481 ctcaaacggg cgctacaacg taatggtgac gactgcgggc ggcggatatt cgcagtgcga
     3541 cggcaacgcc gttacacgat ggcgtgaaga tgttacgcgg gataactggg gcgcttttat
     3601 ttatctgcgg gatgtgcgca gcggcacaat ctggtcgccg ggttaccaac caacacaaaa
     3661 gaagcctctc tcctatcacg ttgccttctc agaagacaaa gtagaggttc gtcgtaccga
     3721 tcccggtatt gccacgcgaa tggagatcct cgtatctgcc gaggacaacg ctgagatccg
     3781 tcgcatatct ttaacaaaca cttcaacgcg cgtcagagag atcgagctca cgagttattc
     3841 ggagatagtg ctggcatcac ctcaagctga tgcagcccat ccagcgttta gcaatttatt
     3901 tatcgagact gagtgcatcg tgaacgaaaa tgcgcttatc gctcaccgtc gtcgaagatc
     3961 gagcgacgaa caacaaatct ggggtatcca tgtcgtcgtc attgagggcg aactgatagg
     4021 cgcgttgcag tatgagactg accggggacg ctttcttggc cgaggtagat caacaaacaa
     4081 tccgatagca gtcatggaag atcggccgct ctcgaacacc actggagcag tccttgatcc
     4141 ggtctttagt ttgcgacgaa gagttcgtat ccaacctaac gaaaccgtca cctgttcgtt
     4201 taccaccggc gttgcctttt ctcgcgaaga agctctagcg ctagcagaca aatatcacga
     4261 cccgaacatt tttgagcgtg agttgcgatt gtcctggacc aaagcccagg tcgagatgag
     4321 ccacttaagc atcgattcgg aagaagctca tctgttccag cgattggcag cccggattgt
     4381 ttactccgat ccatcgctgc gaccccaacc gcatgtgttg gcacttaaca caaaggcaca
     4441 atcaagcttg tgggcgtacg gcattagtgg cgatttacca atagtcgtag tgcgcattga
     4501 tagtgaaggc gatctgggaa cagtcaggaa actggtgcga ggtcatgaat atctccacta
     4561 caaaggcttg agagtcgacc tgattgtcct aaacgacagt ccgacgaatt accaacaaca
     4621 actgcatcac gagctggaaa caatcattcg caccagtgga cttcagggcc ttcaggacaa
     4681 gcccggcgga gtttatctca gacgttctga ccaaataact gaaccagatc gaattttgat
     4741 ccatgcagta gccagagtcg tcatcgttgc cgatcgaggc tcggtccagg accagatcga
     4801 gcggccccga gtggaagagc ctttgccggc tcctttggtc gcaagacttg cgtcgcaaac
     4861 atatccggaa gctccggttg ttcgtcccga tctcacgttt ttcaatggac tcggagggtt
     4921 ttatcagggc ggacgtgaat acgttacggt actcggagcg ggtcagtgga ccccggcgcc
     4981 atggtccaat gtgatcggaa acgaggatga gttcggattt caggtgaccg agacgggagc
     5041 cggctacaca tggtcagtaa atagtcgtga gaacaggtta acaccatggt ccaacgatgc
     5101 ggtcagcgat ccgccgggag aagccgttta cattcgtgac gaggactcgg gaaccatttg
     5161 gacaccgacg ccgctgcccg tacgggaaaa cgaaccctac accgtcaggc acggacaggg
     5221 ttacaccgtt ttcgaacaca ccagtcatgg aatatctcag gagctattag tgttcacgcc
     5281 gttggacgct tcagtaaaga tctctctgct acgtttacgt aatcgcacac ctcgaacgcg
     5341 aaaactttca ataacaaatt acaacgagct ggtcctcgga gtcgcacggt catcctctgc
     5401 tccctacatc atcactgaga ttgatgaggc tgctgcatcg atcttcgctc gaaacccctt
     5461 caataacgag ttctcgacgc gaatagcttt caacgccact aatgagccac tcgcaagtgc
     5521 gacttgtgat cggaaggaat ttattggacg aaacggaagc ctcgaccaac ctgctggcct
     5581 tcgccgcgtt agccttgcaa accgcgatgg agccggactc gatccgtgcg cggcattgca
     5641 aatccaacta gaacttgccc cacacgaagg tcgagaagtc atcttcttac ttggagaaac
     5701 ggaaacaaag gaaaagtgtc aggaactgat agagaagttc aaacaacctg gtaacgttaa
     5761 tgcagctttt gaaaaagtgc tgagtcattg ggaccagctg ctgggagcag tcgaagttcg
     5821 aactccggat gctgcgctcg acacgatgtt aaatcgttgg ttgttatacc aaacactttc
     5881 atgtcgcgtt ctggcgagga cagcctttta tcaatctgga ggtgccttcg gttttcgcga
     5941 ccagcttcag gacgtaatgg cccttgtgta cagcgctccg gaagtagctc gcaaacaaat
     6001 ccttttggcc gcgtcacatc aatttaaaga aggggacgtt caacattggt ggcatccacc
     6061 caccggccgc ggggtgcgca ccaggatttc ggacgacctc ttatggctgc cctttgttac
     6121 cgccttctat atcaaggtga ccgctgacac atcggtgctt gacgaggtgg tgggctttct
     6181 cgagcagcct atcttgcatg aagacgaaca ggaagcgtac ctccagccat cgattgcgga
     6241 agagactgcc actatctacg aacactgcgc tcgcgcttta gaccgaagtc ttgctgttgg
     6301 accacatggt ctgcctttga tgggcgccgg cgactggaac gacggaatga accgagtggg
     6361 gcagcatggt aaaggagaga gtgtttggct tggctggttc ctctttacaa ctctgaatgg
     6421 ctttctgccg cattgtgaaa tgcgacagga aagtacgcga gtcgaagctt atgagaagca
     6481 ttgcgacaat ctgaaaaaag ctcttgaaga aaaaggctgg gacggagact ggtatcgccg
     6541 tgcgtacttt gatgacggaa caccgcttgg ctcgtcacag aacgaagagt gccggatcga
     6601 ctcgatcgcg caatcgtggt cagtgatttc cggagcatca gaccagtaca gaatgagccg
     6661 ggcgatggct gcagttgaag agtacttgat tcggcgtggt gatggcctcg tcattctgtt
     6721 tactccgccg tttgataaag gatctctcga tcccggatac atcaagggct atgtaccagg
     6781 cgttcgcgaa aatggcgggc agtacactca cgcagccatt tggacgatga tcgctttttc
     6841 aatgctgggt gatggcgaaa aagctggcga actcttctcg cttcttaatc cgataaatca
     6901 tgcttcgacg cgggcgggcc tgcacaaata caaagtggag ccttacgtag ctgtcggcga
     6961 tgtttacgct gttccgcctc acactggtcg aggaggttgg acctggtaca ccggttcggc
     7021 cggatggatg taccgggcgg gtttggagtc gattctagga ttcaaactgc agggagaccg
     7081 tctactgatc gacccggcag tgccgcgctg gtggcgagac tttgagatca cttatcggcg
     7141 gaagaacggt gtgtatcgaa tcaaagttga aaacccatcc ggatttagtc gcggtgtggc
     7201 ctcggtagaa ctcgatggca tagttctgga aaaagatgag atacctttaa caggagatgg
     7261 tcagacacat aatgtgagag tcgtgatggg tgaacgagtg cgtgacacca ctcctgaagc
     7321 ggcggaagaa catgccgggg agacaggaac gagtaagagg ggttaggagt aaaagaagga
     7381 ataggacctg tgggacgtag aggacctatt cttaagcggc ggccatgtca gcttcgctaa
     7441 tgtcgaaatt ggcggacact ttctgaacgt cgtcgtgatc ttcgagagct tccataagct
     7501 tcaacatctg acgcgcgtcc gcaccctcga gacgaatgta gttctgcgga accatctcca
     7561 cttcggcgac ctgcggttca attcccgccc ccttcaccgc ggtgagaact ccatcgaatg
     7621 cttctggtga tgtaatgatt tcaaaattgt cctcgtcgtc gcgcaaatcg tcagcgccgg
     7681 cttcgattgc gagttcaaac aattcgtctt ctgtcttggc ggccttatca acgacgatgt
     7741 aacccttctt ttcaaacatc caacctactg atccactctc accaaggttg ccgccatttt
     7801 tagagaagat atgacgaatc tccgcaaccg ttcgatttcg gttgtcggtc attgactcaa
     7861 tcagcaaggc cacaccgcct ggtccatagc cttcatacgt gatctcttcg tagttcacac
     7921 cctcttcttc gccggtacca cgacggatcg cccgttcgat tgtgtcgttc ggcatgttgc
     7981 cactcttcgc atcgaggatt gccttgcgaa gtcgagcgtt ggcatcaggg tcaccgccac
     8041 ctgttcgagc agagaccgtt atctccttaa taaacttagt aaaaagcttg ccgcgcttgg
     8101 catcgagcgc gcctttcttg tgtttaatac tatgccactt cgaatgtccg gacatttatt
     8161 tcaagttcct tttgaaatat gactgtgttt ttttggcttc gcaatatatc acaagagcgt
     8221 tttcattgct catttggcat tttccaggca gggtcctaac ttggcttact ctgtgcaacc
     8281 ttgtgtcttt tgtgcctctg tggtgagcaa agtcctaaga atgcaaaaaa gctactgcag
     8341 ttgcgcgtac cctttcttag cttcaaccaa aacgggaaga tcttgatcgg catcgcgcca
     8401 tgccgcaaaa aagtcctgat agtactttcg actcgacgcc acatcaccac ttgtagcagc
     8461 cgcacgggcg agctgaagtt gagccagcgc atgagccgga gagaaaaaat ctatcgctcg
     8521 atgttccaga atctttttga attcaacggt tgcttctgtt cccttcttct ggtcgagcaa
     8581 aaccaaacct cgcgcatagt tatttgccag ccccgcgatt attcccagat cgtatcgtcg
     8641 aattgactcg agatactgag tagcctggtc caagttgccg cggtcctttt caatcaacgc
     8701 ctttgtgagc ggccccgata tcatcgtcac gggcaggtct tttggatact ccttcactgc
     8761 ttcgtccagc agagtttggc tcttcgaaac atcaccacac gctgccagcg ttatagcacc
     8821 gctcgacata gagaaccgcc ccttaaaaat tgtcagagcg cgctggacat tctctttggc
     8881 tgcctggcat ttccccacga ccgattcagt ctgcgacaac atgaccaaag tgttagccgc
     8941 attttctttg ctacccttgc tcagaaacat gtcagacgac tgcttagcca actcctcgcc
     9001 cttcttaagt cttccagaaa aagtctcagc ccaagccaaa ttggcgatca gctcgggttc
     9061 gtcaagtttg ccacgggccc attccgtttc ctgtttcatt cgagcctggt cgcctcgcat
     9121 aaacgcaaag acgtaagaat ggtagtgccc tcctaaatat tccggattga ttttgagcaa
     9181 ctcattagct gcctgttctg cttcatccat ccgttctaac cccataaaac tgccgatgag
     9241 gttttcgcgc gcactaacgt tattcggacc aagtcgcacg gcctccagtg cttctttcaa
     9301 agcatcgtcg taacgaccga agtaaagata atttaatgcc aaattgttat gaggtatgaa
     9361 atcggttgga tacagttttg cccacgtctg ctgtgtttcg atcgccttat ctatctcacc
     9421 ggtgacgtag ctgtaatact tttccgtgat gtagagtttt tcgcgttcac tgacacggtc
     9481 gcgcaggtca taagctttct gtacgtaacc cttcgcactt tcaaactggc tctgattgcc
     9541 gtagaagact gcgattcgag cgtacgccat ggcgaagttt ggatcgagat cgactgccct
     9601 ctgatagaag gctaaggctt cccgtacttt gcctttattg cgctcctcgt ttcccatcgc
     9661 gtaagccttc aacgcttcga gcgatgaggt ggtagcctgt tcaatcgcca catcgtactg
     9721 cttaaccgaa ctgagagact cgcccagttt ctgtcgcaac gacgaggctg cggtgccgag
     9781 cgaggaaagc accttctcct tactttcggc ttcaatttgt tccctggcaa taggatctcc
     9841 tgaacgagcg ttcagtgctt cgagcgtaat gacgtagtga ctgccaaggc tcgcgatgga
     9901 cccgaccagc atcgctttga ttccctgacg ctcacaaatt tcctttccga cctctttagt
     9961 gatgcgttca tcagctgaac gacccatgaa acgaagagtt tctcgcactc gatcgtcagg
    10021 aaagatgttc aggaacggcg tttggcccaa ctggaccgag agcgcctgct tcaacgtgcc
    10081 atcgaaaact tgttcgccag tcgtattgat gaactcggta agaagaatag tgtccgagtt
    10141 tgtaagtact ggcttgcgct tgaagtaaaa ataactgccg gaggcgatag ctgcgactat
    10201 gaccaatgct aacagggccg ctatcttgtg gcgcttgatt ccgccaacga tgtactcggc
    10261 actcgatacc tgcgataccg ttttttctgc cgtagtcagc gctctatctt gaaaagttgg
    10321 agctactttt ccgctcgcca gcgatctggt gcgtgaatca ggaggaacag accgttcagc
    10381 ctcaatctcg aactcaacgc gctgtttcag gcggcgtaga tccgtaagaa cttccttgat
    10441 cgtctgatat cgttcttcgc gatcctttcg cagagcttta gtcactatcc aatcgagttc
    10501 agccggggcg tccggagcaa atcgagcgag aggtggcggc tctttctgtg tgatgccgac
    10561 gatgacatcg gttggcgttt cgccttcaaa cggtgtccgg cccgcgatca tctcgtagat
    10621 aacgactccc agactccaga tatcactcct tgcatcaacc tgtttaccgc gagcttgttc
    10681 gggcgacatg taatgagatg tgcccatgac tacgccggca tctgtttgga ccagaactcg
    10741 tgtcgaggct tcgccatctg aaggcaaacg atcagtagtg ccctccgtca gtttggcgag
    10801 accaaagtcg agcaccttaa caaacccgtt gcgccggatc atgatgttgt cgggctttat
    10861 atcgcgatga actatgccgg cggcgtgagc ttcctcgagg gcactggcaa tttggacagc
    10921 aatatccaag acctcgcgaa tttcaagatg actggcagcc atctttctcc gcagcgtcac
    10981 cccatcgata tattcggtgg cgatgtaatg gcggccttca tcaactccga cttcgtggat
    11041 tgtcagaatg tttggatgat tcaacgctga cgcggccgaa gcttcgagtt caaagcgact
    11101 taggcggtct cgattcgtcg tgtactcttc actgagaatc ttgatcgcaa ccttgcggcc
    11161 cagcttgaga tcttccgcaa ggtagacctc ccccattccg ccagcaccta atttttcgag
    11221 aattcggtag tgagaaactt tgtcggagtc catttcaata tgaagtcagc caccaaaaac
    11281 taaaaaggaa cgctggagta agtagtgtct taacttggcg cactctgtgc aacctttgtg
    11341 tcctctgtgc ctctgtggtg agcaaagtcc taagaaaacc atcaccacag agaccgaagt
    11401 gtctcagagg cagcacagag aaggttcaga ttaggacgcc actgtaagtt caggcggttt
    11461 gctccttctt ccgtaggata cccaaattcc tcattgcgtc gaagtctgga ccatcaactt
    11521 gcacttgttt cgttcttgag ctaacacctc gaagaatcct tacggcgacc cgaggcactt
    11581 taaaggttct ggcaagcacc tgtatcaact cgtcgtttgc ggcgccatca accggagccg
    11641 aagctaccct tactcgaagc gatcgatcga attcaccaac cacttcactg cgcgaggctc
    11701 ttgggaccac acgtaccttc aataccagac tgtcgttagc atacgtgtgt tcaatcaaaa
    11761 ggtgcgtctc acctcaatct tccatcggaa agcaaagttc cccagatagc cccctgaatc
    11821 agccagagag ccagaagtgc aactaatgga gaaatatcaa acattcccaa tggcgggatc
    11881 agtcgtcgca aagggccaag cagaggttcg gtcacggtaa ccagaaaacg cattatccga
    11941 ttggaataac tcacgcgaac ccaggagaag atgatccaca tcgtgataaa gaggatataa
    12001 acggaaatgg caccattaag aatatggccc acgaccaaca ctggacgacc atcttgaatt
    12061 ccatcgagta cacccagtgc ggtactgccc agcttcccgg ccaattggtt aacaaacaat
    12121 cccagaacaa tagtgaccag gatgaccacc agtggagcgt acttcgggtc aaatcccagg
    12181 ctcatcaagc cgcgtcgcat gggaagtacg aacacatccg tcagccgatg aatggtccgt
    12241 gacgcccacg cgaacggatt gagatttgag gcatttgcga tgagtcgaag aatcattaaa
    12301 gctatgatga cgatgatgat cgctgtcaca gccatgctca caaactcaaa tgtcctggca
    12361 ataatcaaca tatagacttg aaacttcttt tcttgtaaac gttaacctcg ggagccacgt
    12421 tccccgaaga tggcggtacc gactcgaacc atagtagcgc cttcttcgat agcaacctca
    12481 aaatcgtgg
//
LOCUS       DASSFP010000068         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1952883, whole genome shotgun sequence.
ACCESSION   DASSFP010000068 DASSFP010000000
VERSION     DASSFP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952883"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..280
                     /locus_tag="VFH91_01400"
     CDS             35..280
                     /locus_tag="VFH91_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861661.1"
                     /translation="MSDVLDLRITTTVFTTKRPASGGTQITATRPYGQIRNVVVQWKF
                     KKAIFNTTHEVWLISIARYFVLAWRYSHEESEELDAG"
     gene            270..557
                     /locus_tag="VFH91_01405"
     CDS             270..557
                     /locus_tag="VFH91_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861662.1"
                     /translation="MQDNELERNLDKQLVLEGFALNAKAGAVVMTDSNTVVYVTDLQE
                     WPEDFVNDRVEIKGVLHRGRVYPDVEVENGVSSQGLPGDQYYIDMEGYRKA"
     gene            582..>2625
                     /locus_tag="VFH91_01410"
     CDS             582..>2625
                     /locus_tag="VFH91_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HET6861663.1"
                     /translation="MSEHVTTQAAAPAVSPAQLTNGVVLQRKCACGSYGEGGECDKCR
                     KGNGLQRKARGSAVPPALPAEVDHVLGSSGQSLDPAIRRWMEPRLGHDFSSVRVHTDA
                     AAAESARKVNALAYTVGEDVVFATGQYSPNTKAGGTLLAHELTHVVQQAKTPASRAQH
                     SKAISDASDAAEIEADNAAPRLLQGEEVEVNESPNATLHALTPGETTGVVIGSIVGSA
                     GIAAGIAWLAGAFDKESFSDTELLEYVTALAKDRKIQKGSTSDNKARDLVGKWKVNET
                     LDLDKEFKAKEGSLTIVELKRLLILEMLDGPTGESDERSIITIFKKSSESEIKELLTP
                     TLGLSLQDIESDLSEENLKELMAVLNEKLPTIGEPHVKRSETPGKKSGACTVGRAIKI
                     EFAQKQAESIVARTVQVLDSFISNPAGNKTIQDQLNCYFNGPTQNEVKRVRDDFQQIS
                     TTLPLLTYICPAEPFEEFKAGGRVFKQEKDLEARSLVVKEEAKPGAPASPTPTPEAPG
                     LPVIIFPEFFDAPPRHQARVFIHEAFHHAKKQGDEVPEESKHYNPSCGVLSKDEAFIN
                     ADTYATFAMKLTPEGVLSKLDGQNQKGVQRKAAEPAPSAVPSIVQDVVAASGEPLAEP
                     TRAFMEPHFGHDFSDVRVHTGSIAAESARALNATAYTVGEDIVFNSEQYEPTTARGN"
BASE COUNT          730 a          645 c          711 g          539 t
ORIGIN      
        1 aagacaggca aagtcaacat gagcgaatac cactatgagc gacgtcctgg atttacgaat
       61 tacgactacg gtctttacga cgaagagacc ggcaagtggt ggcacgcaaa tcactgcgac
      121 acgaccctac ggtcaaatcc gaaatgtggt ggtccaatgg aagtttaaga aagcaatctt
      181 caatactact cacgaggtct ggctgatttc gatcgccagg tattttgtgt tggcgtggcg
      241 gtacagccat gaagagagcg aggaactaga tgcaggataa cgaactggaa agaaacctgg
      301 acaagcagtt ggtgttagaa ggttttgcac tgaatgcaaa agctggagcc gtggtaatga
      361 ccgactcaaa taccgtggtc tatgtcaccg atctccagga atggcctgaa gacttcgtca
      421 acgatcgtgt cgagatcaaa ggagttcttc atcgcggtcg ggtctatccc gacgtagagg
      481 tggagaacgg agtcagcagt cagggcttac ccggcgacca gtactacatc gatatggaag
      541 gctatcgaaa agcttagggt gttgtcgcgc ataacgtcgc catgagtgaa catgtaacaa
      601 cacaggcagc agcaccggca gtgtcacctg cacaactgac gaatggcgtc gtcttgcaac
      661 gtaagtgcgc gtgcggcagc tacggcgaag gcggcgagtg cgacaaatgc cgcaagggaa
      721 atggactgca gcgtaaagct cgtggcagcg ctgtgccccc agcgttgccg gccgaagtgg
      781 accatgtgtt gggttcatcg ggtcagtcgc tcgatccggc aatccgtcgc tggatggagc
      841 cccggttagg ccatgacttc agcagtgttc gggtacatac ggacgccgcc gcagcagagt
      901 cagctcgaaa ggtcaacgcg ctcgcttaca cggtaggtga agatgttgta tttgcgactg
      961 gacagtactc gccaaacaca aaagccggcg gcaccttact ggcacacgag ctgacccacg
     1021 tagtgcaaca agcaaaaacc cctgccagtc gagcgcagca ttcgaaagca atcagtgacg
     1081 cctctgatgc tgcagagatc gaagccgata acgccgcgcc tcgcttgttg cagggagaag
     1141 aagttgaggt gaacgaatca ccaaatgcaa cgttgcatgc tttaactcct ggcgaaacta
     1201 ccggagtggt aatcggttcc atcgtaggct cagctggaat cgcagcaggg atcgcatggt
     1261 tggctggagc gttcgataag gaatcgttca gcgatacaga gctgctcgag tatgtgacgg
     1321 cgctggcgaa ggatcgaaaa attcagaaag gatctaccag tgataacaaa gctcgagacc
     1381 ttgtgggcaa gtggaaagta aatgaaacgc tcgaccttga taaggagttc aaagcaaaag
     1441 aaggttcgtt gaccatagtt gagctgaagc gactactcat tctggaaatg cttgatggtc
     1501 caaccggcga atctgatgag cgttcgatca ttacgatatt caaaaaatcg tccgagagtg
     1561 agatcaaaga actcctaact ccgacccttg gtctttcact tcaagacatc gagagtgacc
     1621 tcagcgaaga aaatcttaag gaactcatgg cagtactcaa cgagaaactg ccaactatcg
     1681 gagaacctca tgttaagcga agcgaaactc ccgggaaaaa atccggagcg tgcactgtcg
     1741 gcagggcgat aaagatcgag tttgctcaaa agcaagctga gtctattgtc gcgagaactg
     1801 ttcaagtgct tgactcgttc atctcaaacc ccgccggaaa taagactatt caggatcaac
     1861 tcaattgtta cttcaatgga ccaactcaaa atgaagtaaa gcgtgttcga gatgacttcc
     1921 aacagataag cacaacctta ccgctcctta cctatatatg tccggctgag ccatttgaag
     1981 aatttaaagc cggtggccgg gtgttcaaac aggaaaaaga cttagaagcg cgttcgcttg
     2041 ttgtcaaaga agaggcgaaa cccggcgccc ctgcatctcc aactcctacg cccgaagcgc
     2101 caggtctccc agtaataatc ttccccgagt tctttgatgc accgcccagg catcaggcgc
     2161 gtgtcttcat tcacgaggct tttcatcacg caaaaaagca gggtgacgaa gttcccgagg
     2221 aatccaagca ttacaaccca tcatgcggag tgcttagcaa ggatgaggcg tttattaacg
     2281 cggacacgta tgcaacgttc gcaatgaaac tcacgcctga aggtgtgttg tcaaagctgg
     2341 atggacaaaa ccagaaaggg gttcaacgga aggcagctga gccagctcct tcagcagtgc
     2401 cttcgatcgt ccaggacgtt gtagcagctt ctggcgaacc gttggcggag cccacgcgcg
     2461 catttatgga gcctcacttt ggtcacgact tcagtgatgt gcgtgtccac actggttcaa
     2521 ttgccgccga atcagcgcga gctctgaacg cgaccgcata cacagtcgga gaagatattg
     2581 ttttcaacag cgaacaatat gagccgacaa cagcgcgagg caata
//
LOCUS       DASSFP010000069         7100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_607619, whole genome shotgun sequence.
ACCESSION   DASSFP010000069 DASSFP010000000
VERSION     DASSFP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607619"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFH91_01415"
     CDS             complement(<1..562)
                     /locus_tag="VFH91_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6861664.1"
                     /translation="MQITSRDNSLLRKARAVRDGKDDDFVFVEGLRLCEEALRSKLEI
                     YAVLVSEELAGKQRVQAKIEEIASNSKRTGYVSEKLLESISFTKTPQGIVVLAQRPEA
                     GKSLLLSRLGPIPLLLVLHEINNPVNVGAIIRTAEAGGVSGVVTTVKTSDPFSPKALR
                     GAMGSAFRLPFWTDAAFKELLELCREN"
     gene            complement(565..1494)
                     /locus_tag="VFH91_01420"
     CDS             complement(565..1494)
                     /locus_tag="VFH91_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6861665.1"
                     /translation="MVTAIPPLSDSEKLEFGPAVTSVQQPTPNSIVIASYNIRYARGP
                     HLISGGLVRKAGLMSLKQRPSFIAKQISLAARAFSEGKLLPRPHVLLLQEADKRTARA
                     GGHHVAQELANQLEMNWVHAPAGIPRGIKPVQRQWWLDFEEPIGLHDSGDTGVAFLSK
                     LPFSDVTRIDLPWHECPWRPRLAIAATVALGSSKLRIFNAHVDPHAAVDGQLEQMEVI
                     AEEAERTSDPSLILGDFNTLSKKKSIETRAFLESRGYSTSFPTGTPTWRGAGLRLHAD
                     WIFGRGIKITKWGVARPLSVSDHWPIWVELSTV"
     gene            1601..1969
                     /locus_tag="VFH91_01425"
     CDS             1601..1969
                     /locus_tag="VFH91_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861666.1"
                     /translation="MTKRILAVVDDMFFASKIRAVAESVGSDLAFTRSLDALIEKARE
                     KRPDLIVVDLHNQKTSPISIAQQLRSDPELKDVHLLGFFSHVEVELQRQAKEAGYSQV
                     LPRSVFARDLPSILGDVSQQ"
     gene            complement(1956..2963)
                     /gene="aroF"
                     /locus_tag="VFH91_01430"
     CDS             complement(1956..2963)
                     /gene="aroF"
                     /locus_tag="VFH91_01430"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6861667.1"
                     /translation="MKADATDRQVEEVLQVIEELGFKGHPMPGATRTAIGITGNQGAV
                     DPSHFENLAGVAEAIRVSKPYKLITLDLRPEKTVVRVGDATIGGDELAIIAGPCAIES
                     RTQAFAIAEAVRRSGVRFFRGGAYKPRTSPYAFQGLGEEGLRILADVREAYGLKIVTE
                     ALDEAGVDLVEKYGDVIQIGARNMQNFSLLRRAGKARIPVLLKRGLSATLDEWLLAAE
                     YVMAEGNYQVVLCERGIRTFAQHTRNTLDLAAIPAVRRISHLPVIVDPSHGTGKNFMV
                     TPLARAGVAVGADGLIIEVHDQPEHALSDGAQALTLEQYEQLVSDVRSIHEVIAPATV
                     G"
     gene            complement(3024..5099)
                     /gene="argS"
                     /locus_tag="VFH91_01435"
     CDS             complement(3024..5099)
                     /gene="argS"
                     /locus_tag="VFH91_01435"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET6861668.1"
                     /translation="MNLIDLQQILKERISAAALEVFGIEIAEPNAEIPPKPELGDVAF
                     AVSFELAKLIKQKTGEKQAPRAIAEKLKEKLENVELVDRVEIAGAGYINVFFNRAKLL
                     KIFARAAKEDRQRTASPDQPKKMVEHTSINPNKAAHIGHVRNAVLGDTFARVLKAAGE
                     RVEVQNYIDNTGVQVADVVVGFLHIENLNLDQIKQLDSSLTREYPFDYYCWDLYTKVG
                     LFYRDGNPAGDINPEKAKLRTEVLHALEEGGDNPIAELADYVATRNVECILNTMERLG
                     IRYDLLARESDILHLHFWERAFDLMKTKGVIKYETEGKHAGCWVMPFESHTGTDEHEA
                     DKIIVRSNGTVTYTGKDIAYQLWKLGKLGLDFNYKVFRTYDDGVKLWVTTSNPAPDTN
                     EDRPDFGGGVTVYNVIDSRQSYPQEIVARGVAAIVPEVGAEASVHFSYEMVALSPAAC
                     DELGIELTPEDRSRPYIEMSGRKGLGVKADDLINQLEAGALEEVKQRLEEIKKRNPEF
                     SEVDMQSASHEIAVAALRYFLLKFTRNSVIAFDFKEALSFQGETGPYCQYAAVRINSI
                     FRNLGNDLIAETDALLAASENDPNVSAQIRAVLEGDGGTDIWSLLMLAQMLDENVRQC
                     TTAAEPTSLAKYTFNLAKSFNGFYRDHKMMSEPDPIKRGVRILVTGVVKTQLNAALSI
                     LGINVPTQM"
     gene            5276..6037
                     /locus_tag="VFH91_01440"
     CDS             5276..6037
                     /locus_tag="VFH91_01440"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET6861669.1"
                     /translation="MKTVAISNELRIRFAQARNVLVLTGAGVSAESGVPTFRGGGNSA
                     VWKGMPFDVISSAEMVQRNLAAVWEWFDYRRGALESLKPNPAHLEIARWPQRFSEFTL
                     VTQNIDGLHQQAGSLDVIELHGNIWRARCVTCQFRQQMEMGSVREDSCQECGDRLRPD
                     VVLFGELLPRGAFEEAALRASRCDLCFVVGTSALVYPAASLPEIAKKGGAYVCEINPE
                     RTALSDLCDEVINGKAGEIIPAFSEITGKESRLTG"
     gene            6044..>7100
                     /gene="larC"
                     /locus_tag="VFH91_01445"
     CDS             6044..>7100
                     /gene="larC"
                     /locus_tag="VFH91_01445"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET6861670.1"
                     /translation="MKTLYFDCFAGASGDMILGALIGAGVSEKALVEQLSLLNVDGFK
                     LTMETVDRSGLSATFARVHTRHEHKHRHLSDIKKIIYDSDLPVTVKDQAFATFTRLAE
                     AEARVHNEPVDHVHFHEVGALDAIVDVVGAAICFELLQIEKFISSPLHVGSGMIDMAH
                     GSFPVPPPAVTELLKGVPFYSADVKGELLTPTGAAIITSVCSQYGPIPQMIIDQTGYG
                     AGSREYKDFPNVLRVMIGETETEGALDERLWMIETNIDDASPQIIGHVMDRAFELGAL
                     DCFFTPVQMKKNRPGVLLSILSDARTRESVMNLLFTETTTLGVRSYQVLRRALQRSVV
                     AVETQYGRIDVKVARLNG"
BASE COUNT         1648 a         1796 c         1843 g         1813 t
ORIGIN      
        1 tattttccct acacagttcc aacagttcct taaaggcggc gtccgtccag aaaggtaacc
       61 gaaaagcgga gcccattgca cctcgcaacg ccttaggcga gaagggatca cttgttttca
      121 ctgtggtgac tactccacta actccgcctg cttccgcggt tcttataatc gccccgacgt
      181 ttaccggatt atttatttcg tgcagcacaa gcagcaatgg aattgggcca agccggctca
      241 gcaagagtga ctttccggct tcaggccgct gagcaagtac tacaattcct tgaggagttt
      301 ttgtgaaaga gatcgactcg agcaactttt cgctcacgta accagttcgc tttgagttgc
      361 tggcaatctc ttcaatcttc gcctgcacac gttgcttgcc agcgagctct tcggaaacga
      421 gaactgcata gatctcaagt ttggatcgca gcgcttcttc gcagagtcgc aggccttcga
      481 caaagacaaa gtcgtcgtct ttaccgtctc ttactgcccg tgctttgcgg agcagggaat
      541 tgtcgcgact ggtaatctgc atttctaaac tgtgctgagc tcaacccata tgggccaatg
      601 atcggaaacg cttaagggtc tcgcaacccc ccactttgtg atcttgattc cccgaccaaa
      661 aatccagtca gcgtggagtc gtaaacctgc gcccctccag gtaggcgtgc cggtaggaaa
      721 acttgtggaa tagccgcgag actcgagaaa cgctcgtgtt tctatggatt tctttttcga
      781 cagcgtattg aagtccccaa gaatgagaga agggtcggaa gttcgttctg cttcttccgc
      841 gatgacttcc atctgctcaa gttgcccatc tacggccgcg tgtgggtcga catgagcatt
      901 gaagatcctt aacttggaac ttccaagtgc gacagttgcg gctattgcca ggcgcggtcg
      961 ccagggacat tcgtgccagg gcaagtcaat tcgggtgacg tcactgaaag gcaacttgct
     1021 caggaatgcc acacctgtgt caccgctatc gtgcaaccca attggctctt caaaatcgag
     1081 ccaccactgt ctctggacag gcttgatgcc gcgagggatt ccggccggcg catgcaccca
     1141 gttcatctcg agctgatttg ccaattcttg tgcgacatga tggccacctg ctcgtgcagt
     1201 tcttttgtcg gcttcctgaa gtagcagcac gtgcggacgg ggtagcagtt tgccttcaga
     1261 aaatgctctt gccgccagtg agatctgttt ggcgatgaaa gaaggacgtt gtttaagact
     1321 catcaggccg gctttgcgaa cgaggccacc cgaaatcaaa tgaggtccac gtgcataacg
     1381 gatattatag gatgcgatga cgatactgtt tggcgttggt tgttgaacac tggtgaccgc
     1441 tggtccaaat tcaagttttt cgctgtcgct cagcggcggg atcgcagtga ccataggtta
     1501 gctatattca gatgaaagcg ttcagaatac aaatcgttgt aagcagcgag tgtgagaaga
     1561 tgaacctttc gaataaactg gatctccggg aaattacgct atgactaaac gaatactcgc
     1621 agttgtggac gacatgttct tcgcatcgaa aatcagggcc gtcgctgagt cggtgggctc
     1681 agacctggct tttaccagga gccttgatgc cctgatcgag aaggcgcgcg agaagagacc
     1741 cgatttgata gttgtggatc tacacaatca aaagacttca ccaatttcta ttgcccaaca
     1801 acttcgctcc gatccagagc tgaaagacgt tcatctactt ggtttctttt cacacgtgga
     1861 agtcgagttg caacgccagg cgaaagaggc aggatattcc caggtgctgc cgagatcagt
     1921 ttttgcgcgc gacctaccat caattcttgg cgatgtcagc caacagtagc cggcgcaatt
     1981 acttcatgga ttgaccgcac gtcgctaaca agttgttcgt attgttcgag cgttaatgcc
     2041 tgcgctccat ctgacaacgc gtgctcgggt tgatcgtgca cttctatgat tagaccatcg
     2101 gctccgaccg caactcctgc acgagccaat ggagtgacca tgaagttctt tcccgttccg
     2161 tgcgaaggat cgacgattac cggcaagtgt gagattcgac gcacagccgg aattgcagcg
     2221 aggtcgagag tatttctggt gtgctgcgca aaagtgcgaa ttccgcgctc acacaagacg
     2281 acctgatagt ttccttcggc cattacatac tcagccgcga gtaaccattc atcgagggtc
     2341 gcggatagtc cacgcttaag cagaacaggt atacgagcct tgccggctcg gcgcaataac
     2401 gagaagttct gcatgttgcg cgcaccgatc tggattacat cgccatactt ttcgaccaga
     2461 tcgacgcccg cctcatccag agcttcggta acgatcttca agccgtaagc ctcgcgaacg
     2521 tcagccagaa tccttaagcc ttcttcgccc aatccctgga acgcgtaagg cgaggttcga
     2581 ggtttgtagg ctccacctct gaagaaccgg acaccgctac gacgtacagc ctcagcgatc
     2641 gcgaaggcct gcgttctgct ctcgatagca cacggaccag cgatgatagc cagttcatcg
     2701 ccgccaatag ttgcgtctcc aactctgact accgttttct cgggtcgcag atccagcgtt
     2761 atgagtttgt aaggtttgct aacacggata gcctcagcta cgccggccag gttttcgaag
     2821 tgcgaaggat caaccgctcc ttgattccca gtgattccga tcgccgtgcg tgttgcaccg
     2881 ggcatcggat gacccttgaa acccagttct tcaataactt gcaggacctc ttctacctgt
     2941 cggtccgttg cgtcagcttt cattacgatt aacatagttc ttttatactc tcaggcttaa
     3001 tcaaaagtac agaaatttgc tcttcacatt tgagtcggaa catttatgcc aaggatcgac
     3061 aaggcagcgt tcaattgagt tttgacaacc ccggttacga ggattcggac gcctcgcttg
     3121 ataggatcag gttcactcat catcttatga tcccggtaaa agccattgaa cgatttcgcg
     3181 agattgaagg tgtacttcgc tagactggtt ggttcggcag ctgtcgtgca ctgccggacg
     3241 ttttcgtcga gcatttgcgc cagcatcagc aaagaccaga tgtcggtacc cccgtcaccc
     3301 tcgagcacag ctctaatttg ggccgagacg tttggatcgt tctcgctcgc tgcgagtagg
     3361 gcatccgttt ctgcgatcag atcatttccc aggttccgaa agatcgaatt gatccgcacc
     3421 gccgcatatt ggcaataagg acctgtttca ccttgaaaag aaagtgcttc tttaaaatcg
     3481 aaggcgatca cggagtttcg tgtaaatttc aataggaagt aacgaagagc agcaacagca
     3541 atctcatgtg aagcagactg catgtcgacc tctgaaaact caggattccg ttttttgatc
     3601 tcctcgagtc gttgtttgac ttcctcaagt gcacccgctt cgagttggtt gatgagatcg
     3661 tcggccttca caccgagtcc ttttcggccg ctcatttcga tgtaaggtcg tgagcgatcc
     3721 tccggagtta attcaattcc gagttcgtca catgctgccg gcgaaagcgc taccatctca
     3781 taactaaagt ggacgctcgc ttctgcgcca acttccggaa cgatggcggc cacaccacga
     3841 gcgacaattt cctgtgggta cgattgacgt gaatcaatga cgttgtaaac cgttacgccg
     3901 ccaccaaaat ccggtcggtc ctcatttgtg tcgggagcag gattggacgt agtgacccag
     3961 agcttgactc catcgtcata tgttcgaaag accttgtaat tgaagtcgag tccgagtttt
     4021 ccaagcttcc aaagttgata ggcgatgtct ttaccggtgt aagtgacggt gccgtttgag
     4081 cggacaataa tcttatccgc ttcatgttcg tcggtaccgg tgtgcgactc gaacggcatt
     4141 acccaacatc cagcgtgttt accttctgtc tcgtacttga tgacgccttt tgtcttcatc
     4201 aaatcgaacg ctctttccca aaaatgaaga tgaagaatgt ctgactctcg tgccagcagg
     4261 tcgtaacgaa ttcccagacg ctccatggtg ttgagaatgc attcgacgtt tcgcgtcgcc
     4321 acataatctg caagttcggc gattggattg tcgccacctt cttcgagagc gtgcagcact
     4381 tccgttcgga gttttgcctt ctcaggattt atatcgccgg cgggatttcc gtcgcggtag
     4441 aagagaccaa ctttcgtgta aaggtcccag cagtaatagt cgaatggata ttccctggtg
     4501 agagaagagt cgagctgctt aatctggtcc aggtttaggt tttcgatatg aaggaagccc
     4561 acaaccacgt cggcaacctg gacaccggtg ttgtcgatat agttttgtac ctctacacgt
     4621 tctcctgctg ccttgagaac acgagcaaat gtgtcaccca gaacggcgtt gcgaacatgg
     4681 ccgatgtgtg ccgctttgtt tggattgatg ctggtgtgct caaccatttt cttcggttga
     4741 tcgggacttg cggttcgctg tctgtcttct tttgcagccc tggcgaagat cttaagtaat
     4801 ttggctcgat tgaagaaaac gtttatgtag ccagccccgg caatttccac tcgatcgacc
     4861 aactcgacgt tttcgagttt ctcttttagc ttttcggcga ttgctcttgg agcttgtttc
     4921 tcgccggtct tctgcttgat caatttggct aactcaaaag agacggcaaa ggcgacgtct
     4981 ccgagttcag gcttcggtgg aatctcggca ttgggttcag ctatctcgat accaaaaact
     5041 tcgagcgcgg cggcgctgat tcgctctttg agaatctgtt ggaggtcgat caggttcata
     5101 ggcttcgaat gatgcgatca caaaagcgga gagtataccg gagggaagag cgttttcgta
     5161 attggagggg cgtaaaaggt agtggttcag cggtggcgaa caccttcggt gtaaacctga
     5221 atgagtttcc tgctcatttt cctgcctccc tgaaggggtg ttaccatcgc aattcgtgaa
     5281 aacggtagcg atctcaaatg agttacggat tcggtttgcc caggcaagaa acgtccttgt
     5341 cttgactggc gcgggagttt cagctgagtc aggagtccct acattccgtg gcggtggcaa
     5401 ctccgcggtc tggaagggaa tgccttttga cgtcatttca tcagcggaaa tggttcaacg
     5461 taatttggcg gcggtttggg aatggtttga ttaccgtcgg ggtgcgctgg aaagtcttaa
     5521 gccaaatccc gctcatctgg agattgcacg atggccccaa cgattttcag aatttaccct
     5581 ggtgacccaa aatatcgacg gtctacatca gcaggctggt agcctcgatg taatcgaact
     5641 tcacggcaat atttggcgcg ccagatgtgt cacttgtcag tttcgccaac agatggagat
     5701 gggctccgta cgtgaagatt cctgtcagga atgcggcgac agacttcgcc cggacgtcgt
     5761 actgtttggt gaacttctac ctcgaggcgc atttgaagag gccgcactaa gggcgtcgcg
     5821 gtgtgatctt tgtttcgtcg tgggaacatc tgcgctggtc tatccagcgg ccagcttgcc
     5881 ggagatcgca aagaaaggcg gcgcgtatgt atgcgaaatc aatccagaaa ggaccgcgct
     5941 ttcagatttg tgcgacgagg tcattaatgg caaggcgggt gagatcattc ccgccttcag
     6001 cgaaataacc ggaaaagaat cacgccttac aggataacgt tgaatgaaaa cgctttactt
     6061 tgactgtttc gcgggcgcaa gcggcgacat gatcctagga gccctgattg gtgccggcgt
     6121 cagtgaaaaa gctctggttg aacaactttc actgctgaac gttgacggat tcaagctgac
     6181 tatggagacc gtcgatcggt ctggactaag tgcaacattc gcgcgagtcc acacgcgcca
     6241 cgaacataaa caccgtcacc tctcggatat aaaaaagatt atttatgact ccgatttacc
     6301 agtaactgta aaggaccagg cgtttgcgac ctttacgaga ctggcagaag ctgaggcacg
     6361 cgtccataac gaaccagtgg accatgtcca ctttcatgag gttggagccc tggacgcgat
     6421 tgtagacgtc gtgggggctg ccatctgttt tgaattactg cagattgaga agttcattag
     6481 ttctccactc catgttggaa gcggcatgat cgatatggcc cacggtagct ttcccgtgcc
     6541 gccacctgct gttaccgaac ttctaaaggg cgtgccattc tattccgccg acgttaaagg
     6601 cgagttattg acgccgactg gcgccgccat cattacctca gtttgcagtc agtatggacc
     6661 aataccgcag atgattatcg atcaaacagg gtacggcgcc ggctcacgcg aatataaaga
     6721 ctttcccaat gtcttgcgtg taatgattgg agagaccgaa actgagggcg cgctagacga
     6781 acgcttgtgg atgatcgaaa ccaatatcga cgatgcttca ccacagatta ttggacatgt
     6841 tatggatcgc gcgtttgagt tgggagcttt agattgtttc tttacgccgg tgcagatgaa
     6901 gaaaaatcgt ccgggtgtgc tgttgtccat tctcagcgat gctcggaccc gggaatcagt
     6961 catgaatctg ctttttaccg agacgacaac cttaggcgta cgaagctatc aagtgctgag
     7021 aagagccttg caacgaagcg tcgtggcggt cgaaacgcag tatggacgga tcgatgttaa
     7081 agtagctcgc ttgaacggca
//
LOCUS       DASSFP010000070         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1208417, whole genome shotgun sequence.
ACCESSION   DASSFP010000070 DASSFP010000000
VERSION     DASSFP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208417"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFH91_01450"
     CDS             complement(<1..1073)
                     /locus_tag="VFH91_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6861671.1"
                     /translation="MLQLLNISNIALIDSLQVEFDSGLNLLTGETGSGKSIIVDALGV
                     LIGGRFSSELLKSGTDRGSIEGLFTVATNPDVIALLENAGIAGVDSNQESIELIVRRE
                     VSATGRNKIFINNQLATQTLLRLIREYLVDIHGQGEQQTLFNPETHLELLDAFANNTS
                     LRSQVATSFARWKELKQQLKQLNQDEAAQYQLVDTLKFQIKEIQNANIAIGEDEQLED
                     ERRRLSNVEKLTELSQGTYGRIYEDDESVLARLQHALKDLTELAEYETSFASYIEGLE
                     SSRAMLEDLSFSLREFGDKLEFSPARLSFIEDRLGELSRLKRKYGGSLHSVMEHLAGA
                     EDRLRAIETSDQRQGELNQLLRDA"
     gene            complement(1220..1699)
                     /locus_tag="VFH91_01455"
     CDS             complement(1220..1699)
                     /locus_tag="VFH91_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861672.1"
                     /translation="MTDPKRELLRHTVATLAYRGSKAIRNSGEEFGSFTCGPATRTPL
                     QILAHIGDLLEWGLALAKGEQVWRNSSGESWSREAERFHACLTDFDQFLASDAELKAP
                     AERLFQGPVADALTHVGQIAMMRRLADTPMRGENYYVADISTGLTGADQPPPRQEFG"
     gene            complement(2094..2612)
                     /locus_tag="VFH91_01460"
     CDS             complement(2094..2612)
                     /locus_tag="VFH91_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861673.1"
                     /translation="MLGGWQLTGIFRWNSGLPFQTPFDCCFWATNWNVQSNGVRVRPL
                     QAQTNKSGTPNAFADPVAAYRSFRSARPGEVGDRNVLRGPSYISLDSGLYKTFKLPWE
                     GHTLQFRWEVFNVTNTQRFDPLTISDLSLGTDPFLGGNPNSDFGRYTSTQAPLNETKA
                     GRVMQFALRYSF"
BASE COUNT          755 a          771 c          805 g          824 t
ORIGIN      
        1 gcgtctcgaa gaagttggtt tagttctccc tggcgctggt ctgaagtttc gatcgctcgc
       61 aaccggtcct cagcgccggc gaggtgctcc attaccgaat ggagcgagcc tccatatttc
      121 ctcttgagcc ttgacagttc accgagtcga tcttcaatga agctaagcct cgccggagaa
      181 aattccaact tgtcgccgaa ttcccgaagc gaaaaggaga gatcctcaag catcgctcta
      241 ctactctcga gaccttcgat atatgaggca aagcttgttt cgtactcggc cagttcggtc
      301 agatctttta gcgcgtgctg cagtcgagca agtacagatt cgtcgtcctc atagatacgt
      361 ccataagtgc cctgcgacaa ttcagtcaac ttttcaacat tactcaatcg tcgacgttca
      421 tcttcgagtt gctcgtcctc gccaatcgcg atgtttgcat tctggatctc tttaatttgg
      481 aacttaagtg tatcgactaa ttgatactgg gccgcttcgt cctggttgag ttgctttaat
      541 tgttgcttaa gctctttcca gcgcgcaaat gatgtcgcaa cttgtgaacg aagcgaggtg
      601 ttgtttgcaa aagcatccag gagctccagg tgtgtttctg gattgaagag tgtctgttgt
      661 tctccctgtc cgtgaatatc gacgagatat tcgcgtatta agcgtaagag agtctgggtt
      721 gccaattgat tattgataaa tatcttgttg cgcccggtgg ctgagacctc acggcgtaca
      781 atcaattcga tcgattcctg atttgaatcg acgccagcta tccccgcgtt ttccaggagg
      841 gcaattacgt ccggatttgt agcgaccgta aagagtccct cgatggatcc ccgatccgtt
      901 cctgacttca gcaactcgct actgaatcgg ccgccgatga gcaccccaag cgcatcaaca
      961 atgatcgact tgccggagcc agtctcgccc gtgagaaggt ttagaccact atcaaattcg
     1021 acttgaaggc tgtcgatgag agcgatgttt gagatgttca gaagttgcag cattaagagg
     1081 agagtagctt aactgcaaac gcgatgaagc ccaaatcaag taccaattat catttgagaa
     1141 ttatcaatta ctattgtgtg tccgaattaa caatctctca aatatcaatt cataattcat
     1201 aactgattcg attctgccgt caaccgaact cttgtcgggg tgggggttga tcagctccgg
     1261 ttaggccggt ggagatatcg gcaacatagt aattttcgcc gcgcattgga gtgtccgcca
     1321 aacgtctcat cattgctatt tgaccaacat gcgttaacgc atcggcgact gggccttgaa
     1381 agagccgctc ggctggcgcc ttaagttcgg cgtctgacgc gaggaattgg tcaaaatcag
     1441 tcaggcatgc atgaaagcgt tccgcttctc tggaccacga ttctccagac gagttacgcc
     1501 agacctgttc tcccttcgcg agcgcgaggc cccattccag gagatcacca atatgggcga
     1561 ggatctgaag tggagttcgt gtggcgggtc cacacgtaaa gctgccgaac tcttcaccac
     1621 tgttcctgat tgctttcgag ccacgatagg cgagagtcgc tacagtatgt cgtaatagtt
     1681 cgcgttttgg gtcagtcatt ttgtttggtc ccgttgtccg ttactgaaat tgtacttgta
     1741 attcaaattt gaattgtggt tgagatcgtt agatctggat cgatgtcggg ttgtagccgg
     1801 ggtcagtacc gattcgcgat gtcgatggta gccgaggtca gtaccgattc gcggtagcgt
     1861 cgggtctatc tgcatttagt catcaagttg taacccgacg ccgcgaatcg gtactgacct
     1921 tggctaccac ccgacatcaa agtccaagcc tcgattaccc ggaaatagta caaagcacca
     1981 agcacaaagc acaaagtacc aagcacaaac cacagaccag ggacaacacc cacggacaaa
     2041 aagggtcgga gccattttga ctccgacccg aagatcaccg ctcttaaatt agcttagaac
     2101 gaataccgca acgcaaactg cattactcgt ccggccttgg tttcgttcaa gggagcctgt
     2161 gtcgaggtgt aacgtccaaa gtcggagttc ggatttcccc caaggaatgg atccgtgccc
     2221 aggctcagat ccgaaatcgt caacggatcg aaccgctgag tgttggtaac gttgaagact
     2281 tcccagcgga attgcagagt gtgcccttcc cacggaagtt tgaacgtttt gtagaggccc
     2341 gagtctaacg agatgtagct tggaccacgt agtacattac gatcacctac ctcgccaggc
     2401 ctggccgatc ggaagctgcg atatgctgca accgggtccg caaaggcgtt tggtgttccg
     2461 gatttatttg tctgtgcctg gagaggccgc actcgaactc cgttcgattg cacattccag
     2521 tttgtggccc aaaagcagca gtcgaatgga gtctggaatg gtagtccgct gttccaacgg
     2581 aaaataccag tcagctgcca tcctcccagc acggcgtcta cgagtccact cgagctgttc
     2641 agaaacttct tacctcgacc gataggtagg ccgacgaccc agttcgcatt aatgatgtga
     2701 gtagcatcga aatccgagct ggcatatttt aacgggtgac gttcacttcg ttccgctcga
     2761 tttcagggaa cgtaagcgga gaagttggcc cggttggcgt gtgattcttg caagagcttc
     2821 aagaagttaa tgagcacccg cttctcccga gcgtgtgggg gagatactga cgcttctaga
     2881 gggcgcactt tttgtaccaa gttgattcaa gttgatttaa tgcatgtatt ggcgcaaagt
     2941 agagttacat aactccattt atccgaaaag cttgtccaaa aacctgagcg ggcttgctgt
     3001 ttctgaactg cgagattttg taacttgcta cgtatattta gatctgtgga cgaaaatggg
     3061 cattttttaa ggagttgtgg ttaagacgac tagatgtcga tcgtagtccg agaaggagtt
     3121 ctattatggg ctgggatgga gatgacctcg ctgga
//
LOCUS       DASSFP010000071         5805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_165934, whole genome shotgun sequence.
ACCESSION   DASSFP010000071 DASSFP010000000
VERSION     DASSFP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165934"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFH91_01465"
     CDS             <1..261
                     /locus_tag="VFH91_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861674.1"
                     /translation="KVQPTTEIHDATNGIPPTGVGGSLKSNLPEIHGATNGIPPTGVN
                     GCLKFKLSSDGGTLFRRRLVGWILTNHQLPLVGFSGGVTQSL"
     gene            706..1242
                     /locus_tag="VFH91_01470"
     CDS             706..1242
                     /locus_tag="VFH91_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6861675.1"
                     /translation="MASQDDEFTMLFQDFYPGLCRFLECLLGGRAELAQDLAQESFLQ
                     LHRTAWGTFPVGEARFWLYRVARNLAINEFRKSHTRYRLFDRVVEVMRPTVRNPEQEY
                     ETQERKELVLQMLRTLPEDQRVALLLREQEQLSYREIGEVLNISESKVKVDIFRARTA
                     LRDKWTQRAKSVGATAKL"
     gene            1197..2171
                     /locus_tag="VFH91_01475"
     CDS             1197..2171
                     /locus_tag="VFH91_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6861676.1"
                     /translation="MDATREECGSHGEIMICDCTEKISLLVDGELSPNDAREVERHLL
                     TCAECQQTRADFLSVRSELLSFRSNAPQIALRSELTKILAPGNRELPVTSRRRSLVWS
                     FGSAAAFATLIIASAVIALLFVRSSSKQQPVDSRAVATATPQPIANGANAKPSPAPPE
                     ESPKINNPKEQTKEPKNAPASPSRRPGPAPTPRPGEQFALIPEPVRPADAQTMTAMHF
                     EKSEILLRSFRNVRLESGSVAEVKYEKKRAQQLVYQNMILRREADASGDVQSSTLLES
                     LEPILLDIANLPEKPTPNDVQLIKDRVERKNIVPLLQVNSAALARALD"
     gene            2444..3955
                     /locus_tag="VFH91_01480"
     CDS             2444..3955
                     /locus_tag="VFH91_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6861677.1"
                     /translation="MKQMTKYFSRARVGVMLLLIGLSIAIGLHAQRVYGLDQDIAKLN
                     RFVQSSKSNPAAMKMFQEGRDLIESQNWRGAADKFNDFIKGFPKDKDLDAALYWYGYA
                     LQKQNLKDQAKVPLIKLINNFPNSSWRKEAEALLVYLGEQNAVKEQHLQDNCEIKILA
                     LQSLFQADEERAIGIVTESLRSNAANQCKGFQAAAVSVLGGRGGPRVVPILVDIAKNN
                     SDLKLRLTAIKRLGEQHNDQVTDELIKIYDADRTREIRSQILRALVESRTARGTAKVL
                     EVASSGDDPVARLAAIKYIGDLKDINSLDALIRIYDTDKSPQIRSQVLQALSEREDPR
                     ARAKLLEIAKTGETPEIRMDAIRRLGVHGRVALDDLLQLYSSETNMQIKQGLLRAFGN
                     SKDPRAVAKLFEIARGNDPVELRGYAIRQLGNKDDEQTVSQLIAIYDSEQNMQLRGSL
                     LRAFGDSKQKIAVQKLMMIARNDPSVELRKTAVHYLGQSKDPDALRFLEELLK"
     gene            4095..5705
                     /locus_tag="VFH91_01485"
     CDS             4095..5705
                     /locus_tag="VFH91_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6861678.1"
                     /translation="MMRYPKTLGTVSLILTLSAIACAGGSIPTTKAANPINKPGSDEM
                     QTQSATTTIQSFTVVEGNDLMSKLESAQVKARTRQTPYWSAYAFDVRPGVAVDAAFRE
                     FNGTINTLGDTSVMVGTTSNGITVETRNLAVFLLRDPSANQIIRMEVYNLERKREYAG
                     YPVYWLGRANNEESLNYLRALVGAAPLDNLSERAVLGIALHDDVRVGGMLKNLISTSP
                     NPKIRSSSVYWLGQVGGEQAFLASLVSNNAEDAKIRRQAAHAIGNSHDRGTMATLQQL
                     FEGIKDIDIHRSIISAAGASDELQPAFNFLLTVAKGDEDWESRRMAVRQLGRFHTEAG
                     TDELLRIYSSETNVEVKKAALRSLAETKSAKAYARVVEIARNDNNIELRKQAVRVLSD
                     KGEAAIDDLLKLYDAEQAPQVKRSVLQSLGDIKNARVEDKLFEVARNGETVDLRRQAI
                     RLLGERVGKRSFDFLSATAQSSDANADVQVQAVRAIGERRSDESVALLIKIARTHPNQ
                     QVRKQAIRSLGDSGDPRAVEFFREVLTK"
BASE COUNT         1496 a         1396 c         1580 g         1333 t
ORIGIN      
        1 aaagtccaac ctaccaccga gatacacgac gcgacgaacg gaatcccacc aacgggagtt
       61 ggtggatcgt taaagtccaa cctacccgag atacacggcg cgacgaacgg aattccacca
      121 acgggagtta atggatgctt aaagttcaag ctgtcgagtg atggaggaac gctgtttagg
      181 cgtcgtctcg taggttggat tttaacgaac caccaactgc cgttggtggg attctcgggt
      241 ggcgttacgc agtcgttgta ggtaggactt taaggaacca ccaactgccg ttggtgggat
      301 tccccgacta tgcggctgct cctagtagaa aggatctgaa caatccactc actgccgtga
      361 gtgggatttt cggtttttgt gcaaagctgt gtctgatgct ggaagttaca cagtcgctgc
      421 gctcgtggtt ctaatgtggt cagcttgttg actggtggat agcgaagtga ctgtgtaacc
      481 ttcagcatca caagcgcgta gtagcaacag tcgcttcgct catggttcgc gtgagtctcg
      541 aatatcgctg cttgacgaaa cctcgagacc gacccgaccc tgtcgccagt cggtatgccc
      601 tttgttccca caaggcaatg accaataatc ctgatttttc ggccaggatt ctttttttgg
      661 taacttttct cgaacccacg tgttttagga gatgtagcgc agcttatggc gagccaagat
      721 gatgagttca ccatgctgtt tcaggacttc tatccgggcc tatgtcgctt tctggagtgt
      781 ttattgggag gtcgcgccga gctggcgcag gatttggcac aggagagttt cctgcaactg
      841 caccgaaccg cttggggcac cttccctgtc ggagaggccc gtttctggct ctatcgggta
      901 gctcgtaacc tggccatcaa tgagtttcgg aaatcccata ctcgctaccg attgtttgat
      961 cgcgtagttg aagttatgag gccaacagtc cgcaatcccg aacaggagta tgagacgcaa
     1021 gagcgaaaag aactggtgct gcagatgtta agaactcttc ccgaagacca gagagtcgcc
     1081 ttgttgttgc gcgaacagga acagcttagt taccgcgaga taggtgaagt tttgaatatc
     1141 agcgaaagca aagtgaaggt agatattttt agagctcgaa ctgccttgcg cgataaatgg
     1201 acgcaacgcg cgaagagtgt gggagccacg gcgaaattat gatctgcgac tgtaccgaaa
     1261 agatatcgtt gttggttgat ggtgagcttt cgccgaatga cgctagagaa gttgaacgcc
     1321 atctgctgac ttgcgcagag tgtcaacaaa cccgtgcgga ctttctaagc gtccgttctg
     1381 agcttttatc cttccgcagt aacgcgccac agatcgcgct gcgtagtgag ctcaccaaaa
     1441 ttctggcgcc gggaaataga gaattaccag tcacgagtag aaggagatcg cttgtctgga
     1501 gctttggttc agccgcagct ttcgcaaccc tgattatcgc aagtgccgta atcgccctgc
     1561 tttttgtcag gtcatcgagt aagcagcaac cagtcgattc tcgtgctgtt gcgaccgcga
     1621 cccctcaacc aattgcgaat ggagctaatg cgaaaccttc tccagcgcca ccggaagagt
     1681 ctccaaaaat aaacaatcct aaagagcaaa caaaggagcc gaagaatgct ccggcttcgc
     1741 cttcacggcg acctggtcca gctcctactc ccagacctgg agagcagttt gctctgattc
     1801 ctgaaccggt acgtcctgcc gacgcacaaa cgatgacggc aatgcacttt gagaagtcag
     1861 aaattttgtt gcggtctttc aggaatgtac gactcgagtc tggctctgtt gcagaagtga
     1921 agtatgagaa gaaacgtgct caacagttgg tctatcagaa catgattttg cgtcgcgaag
     1981 ctgatgcgtc tggcgatgtc cagagttcaa ctttgcttga gagtttggaa ccgattctcc
     2041 tggatatcgc aaatctaccg gagaagccaa cacccaacga tgtgcaatta atcaaagacc
     2101 gggtcgaacg taagaacatc gttccattat tgcaggttaa ctcggccgcg ttggcccgtg
     2161 cgctcgattg accttgggag tgcggcggcc cggcgccgct ttgtaaaaac gagaactgac
     2221 taatgggttg tggatactac cgggcccggc gccccttgct aaaaaacgac accaacctct
     2281 tgcgattcac ctcagaggca cagggaacat cgagatgtaa tgtgcgttct tgatcaccgc
     2341 tcgatctgct gtgcttgctc ttcgagttct ctgtgcctgt gcggtgagcc gacagattga
     2401 cctcgaggtt gaactcaaga attatgaaac agagaaaaca attatgaagc agatgacaaa
     2461 atacttttcc cgagcaagag ttggagtaat gctcttattg atcggcttat cgatcgcgat
     2521 tggacttcac gcgcaacgtg tttacggtct ggaccaggac atcgccaaac tgaatcgttt
     2581 tgttcaatct tcaaagtcca accccgccgc gatgaagatg tttcaggaag ggcgagattt
     2641 aatcgaatcg caaaattggc ggggagcggc cgataagttt aacgacttca tcaagggctt
     2701 tccaaaggat aaggacctcg atgccgcact ctactggtat ggctatgctc ttcagaaaca
     2761 gaatttgaag gaccaggcca aggttccgct aatcaagcta attaacaatt ttccgaattc
     2821 cagctggcga aaggaggcgg aggctttgct cgtctatctg ggtgagcaaa acgcggtaaa
     2881 agaacaacat ttacaggata actgcgagat taagattctg gcgctccaaa gtctttttca
     2941 ggctgatgag gagcgcgcta tcggtatcgt cactgagtcg cttagatcca atgctgctaa
     3001 ccaatgtaag ggatttcagg cagcagcggt ttctgtcctg ggcgggcgag gtggaccacg
     3061 agttgtccca atcctggttg acatcgcaaa aaataattct gatttgaaat tgcggctgac
     3121 tgcaatcaag cgtctgggag agcagcataa cgatcaggtc acagacgaac tcataaagat
     3181 ttacgatgca gataggacca gggaaatcag gtcgcagatc ctacgagctt tggttgaaag
     3241 tcgcacggct cgaggaactg caaaggttct ggaagtcgcg agcagtggag atgaccctgt
     3301 agccaggttg gcagctatca aatacatcgg agacttgaaa gacattaact cgctggatgc
     3361 gttgattcga atttacgaca ccgacaaatc gccgcaaatt cgaagtcaag tattgcaagc
     3421 tctctcagaa cgggaggatc ctcgagcaag agcgaaactt cttgagatag caaagacggg
     3481 tgagaccccg gaaatccgaa tggacgccat ccgcagattg ggtgtccatg gccgcgtcgc
     3541 cctcgacgat cttttgcaac tctattcgtc cgaaacgaat atgcaaataa agcagggtct
     3601 gttgcgcgca tttggcaaca gcaaagatcc tcgggctgta gcgaaactgt ttgagattgc
     3661 tcgtggcaat gatccggtcg agttgcgcgg atatgcaatt aggcagctgg ggaacaaaga
     3721 tgatgagcag actgtaagtc aactgattgc gatctacgac tcagagcaga atatgcagct
     3781 tagaggctca ttgctacggg cctttggcga ttcgaagcaa aagatcgctg tacaaaaact
     3841 catgatgatc gcgcgcaatg atccttcggt agagttgaga aagacggcag tgcattacct
     3901 gggacaaagc aaagatccag atgcgctgag gtttcttgaa gaattgctca aatagccagg
     3961 ctcctttgtt cgggcggcgt ggtggtgagc tctccaaact catcacgtag ctgactgtcg
     4021 cgccgcccgg caaggaggcg atcaattttc cggcgagggt ccacctcgcc ccaatctttt
     4081 cggggagtcc aatcatgatg cgttatccca aaacgttagg tacggtgtct ttgattctca
     4141 cgctatccgc catcgcttgt gcgggcggaa gcattccaac aacgaaagct gctaatccga
     4201 tcaacaagcc aggctcagac gaaatgcaga cgcaatcggc gacgaccacg attcaaagtt
     4261 ttaccgtagt cgaagggaat gatctgatga gcaagctcga gtcggcgcaa gtgaaagcgc
     4321 gcacacgcca aacgccctac tggtcagctt atgccttcga cgtgcgtccc ggcgttgcgg
     4381 ttgacgcagc gttccgcgag ttcaatggca ctatcaacac gctaggtgat acgtcagtga
     4441 tggtgggaac aaccagtaac ggcattactg ttgaaaccag gaatctcgca gtcttccttt
     4501 taagagaccc ttcggctaac cagataatca ggatggaggt ctacaacctc gagcgaaaac
     4561 gtgaatatgc cggttatccg gtttactggc ttggtcgagc aaacaacgaa gagagtttga
     4621 actacctgcg agcgctcgtc ggtgcggcgc cgctcgacaa tctgagtgaa cgcgcagtcc
     4681 tcggcattgc tttacatgac gacgttcgcg tcggggggat gttgaagaac ctgatttcca
     4741 cttcgccaaa cccgaagatt cgatcatcgt cggtatattg gttgggccag gtaggtggtg
     4801 agcaggcgtt tctggccagt ctggtaagta acaacgctga agacgcaaag atcagacgac
     4861 aggcagcaca tgctattggc aacagtcatg accgcgggac catggccact ctgcaacaac
     4921 tctttgaggg aatcaaagac attgacattc atcgcagcat catttcggcg gccggtgcaa
     4981 gtgatgagct acaaccagcc ttcaactttt tactaactgt cgcgaaaggc gatgaagact
     5041 gggagtcgcg caggatggcc gtcagacagt tggggcgttt tcacacagaa gccggaacgg
     5101 atgagttgct gagaatctat tcaagtgaaa caaacgtcga ggttaagaaa gctgcactgc
     5161 gttcactcgc ggaaaccaag agcgcaaaag cctacgcccg ggtggtcgaa atagccagga
     5221 acgacaacaa catcgagtta cgcaaacaag cagttcgggt cctgagtgat aaaggtgaag
     5281 cagcgatcga cgatttgttg aagttgtacg atgccgaaca ggcgccccaa gtaaaacgat
     5341 cggtgttgca gtcgctcgga gatatcaaaa acgctcgagt tgaggataag ttatttgagg
     5401 tcgctcgaaa tggtgaaacg gtcgacttac gacgtcaggc cattcgttta ctgggagaac
     5461 gggttgggaa gcgcagcttt gatttcttga gcgcgacggc ccaaagttcc gatgctaatg
     5521 ccgacgttca ggttcaggct gttagagcaa tcggtgaacg ccgttcagac gagtcggtag
     5581 cgttgctcat aaagatcgca aggacccatc ccaatcaaca ggtgagaaaa caggcgatcc
     5641 gttcgttggg cgactcggga gatccccgag cggtcgaatt cttccgggaa gtattgacga
     5701 agtagttttg gagtgcgccg gcccggcggc gctttgtaat accagtctct agaccgcaaa
     5761 gaggttttag aaatcgccca tcccaaacgc cttttaagac ttcgg
//
LOCUS       DASSFP010000072         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1953085, whole genome shotgun sequence.
ACCESSION   DASSFP010000072 DASSFP010000000
VERSION     DASSFP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953085"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFH91_01490"
     CDS             complement(<1..200)
                     /locus_tag="VFH91_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861679.1"
                     /translation="MLEPPQMLARSKPWLALAGIVIVSTAALAWSFTRLHPTEKIERV
                     PASGKRGSGSGSLSREPNSPSS"
     gene            980..1570
                     /locus_tag="VFH91_01495"
     CDS             980..1570
                     /locus_tag="VFH91_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861680.1"
                     /translation="MQNMPPMLAYTLRGSTASGIAPLLIVGIGSQATRFTPANPQDDS
                     YWFVFLDRNNPKNKVADFLVPGQNNTAIPPNVDQYMSNPNCIFALATQNLSMLHVPQG
                     DFFNYLVKYGAGRELQRIEQLNSTLGCGSIGRPLYLLTGEGGPRGGTNPPLASYEKGS
                     LANTEYILMSLMPLPNGQPPYSLCESYTWRAAAAHP"
     gene            1651..2442
                     /locus_tag="VFH91_01500"
     CDS             1651..2442
                     /locus_tag="VFH91_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET6861681.1"
                     /translation="MSDEWKFRPGTLDRAIFNGVVYLNEYQLPSLFGLDDVVIDIGTH
                     IGSFAHAAASRGCHHVYSVEPDAANLEIASQNLRSYIANGDVKLLRGAVWRSDSNDDQ
                     LRFDGYHAFPNSHVELAGILNTGNGSVIWGVGNPVEKIAFDDLVDMATNSRRRRIRLL
                     KLDCEGAEWPILLTSQRLHFIDEICGEFHEIGGEYLEISEGREVGEPIFRYAGASLTI
                     NLLIKCLIDAGFRVDYQRHRRPSGALEGLGLFFARRTEKLAEGLR"
BASE COUNT          627 a          638 c          652 g          637 t
ORIGIN      
        1 ttactcgaag gcgagttggg ttcccggctc aggcttccgg accctgagcc ccgctttccg
       61 gaggcgggta cccgttcgat tttttccgtg ggatgaagtc gtgtgaaact ccaggcaagc
      121 gctgcggtgg atacgataac gatgcctgcc aaagctaacc agggtttcga acgggcgagc
      181 atttgtgggg gttctagcat tgcgcctaga ctacgtttgg tctggctcga gcgatacgcg
      241 gatgctatca cggatcgggt agggcaacca ctatctagta ttggaaggta cagcggccac
      301 aatgtggatt tcctcagtcg caaatcgatg gtaagcagcg cgcgcgaagc ggaagatccc
      361 gaattagaac ggtgagcgaa gctaccgatt ggcgtcggct ttatgaaccc gaagggttct
      421 aacgtagccg aatctcagtc accgaagttc gtatcaatgc tgacacggaa aacgacagca
      481 cgacatacca aatcgagtga atagggagcg cgggtaatga acgcaagtac tacctgacga
      541 tcagaactca atccaaaata tcagcgagaa aaagccgatc gttttctgag ttccgtcatc
      601 gtagaatcga ctctcaacgc caatatttat tgtgttgcac ttgaatagct ccgagtcgtt
      661 ccgaatagac ccaaacaaaa acagcctctt cttttttctt ggggatactg cggctgttgc
      721 tgtgttttag gattcatcgt tgttgcttag actgtttaaa ttttaaggcg acgtgatgtt
      781 ctgatgaaga ttgcgtctct cgtttcacaa ttaacgctcg aggagaaggt tctgttggca
      841 tcgaaccttt ttaatcttgc tttgtgtgtc aacgcatgaa gacaggcgaa cacaataaaa
      901 atttgacagg tttctcaagc accaataaca tacctgcggt cctcgcgcat ttttcaccac
      961 ccatactcag gagtgtccaa tgcaaaacat gcctccaatg ttggcttaca cgttacgcgg
     1021 ttccacagcg tcgggcattg ccccactgtt aattgttggt atcgggtcgc aggctactcg
     1081 gtttaccccg gcgaatccac aagacgactc gtactggttc gttttcctgg atcggaacaa
     1141 tccaaaaaac aaagtcgcgg actttttagt tcccggccaa aacaacaccg caatccctcc
     1201 gaacgtcgat caatacatga gtaaccctaa ttgcatcttt gctctggcga ctcagaatct
     1261 gagcatgctt catgtcccac aaggtgattt cttcaactac cttgttaagt acggcgctgg
     1321 gcgggaattg caacgaattg agcaactgaa ttcaacactt ggttgcggat caatcggtcg
     1381 gccactctat ctcttaaccg gtgagggtgg tccgcgtggg ggcactaatc ctccgttggc
     1441 gagttacgaa aagggatcgc ttgcaaacac cgagtatatc ctgatgtcgt tgatgccact
     1501 tcccaacgga cagccaccgt attcgctttg tgagagttac acctggcgag cggcggcggc
     1561 gcacccttaa ccacatccgg ccgccttcct catttcaaag agatggggaa ggtgcgccga
     1621 gattaactgc tgagttgaaa gactcctgaa atgtccgacg agtggaagtt tcgccctggc
     1681 actttggata gagcgatctt caacggtgtc gtctatctca acgaatacca attaccttct
     1741 ctttttggac tagacgatgt cgttatagac attggtactc acattggatc ttttgctcat
     1801 gcagctgcgt caagaggttg ccaccatgtg tattccgtgg agcctgatgc agcaaacctc
     1861 gaaattgctt cgcagaatct acggtcttac attgcgaatg gtgacgtcaa acttttgcgc
     1921 ggtgccgtat ggcgctccga tagcaatgac gatcaactgc gcttcgatgg ctaccacgct
     1981 tttcccaatt cccacgtgga gctcgcaggg atattaaata ctggcaacgg ctcggttatc
     2041 tggggagttg gtaacccagt agagaaaatc gccttcgatg atcttgtaga tatggcaaca
     2101 aacagtcgtc ggcgaagaat tcgtttactc aagctcgatt gtgagggcgc agaatggccg
     2161 atactgctga cgtctcagag gctgcatttc attgacgaaa tttgcgggga gtttcatgag
     2221 ataggtgggg agtatctgga gattagcgaa ggtcgtgagg ttggagagcc aatttttcgt
     2281 tacgccggag cgagcctcac gatcaacctg ctgatcaaat gtcttatcga cgccggtttc
     2341 cgcgtggatt atcaacgaca ccgacgacca agcggcgcgc tcgaaggact gggtcttttc
     2401 ttcgccagaa gaacagaaaa acttgccgaa ggtctaaggt agacgttgat gacaactctt
     2461 ccctcacctc tcaaaatcat cgccatcggt tacaacacct acggaaccgg cttaacgcgc
     2521 gtgatgcacg ccatcatgcg acgcctcgca ggcc
//
LOCUS       DASSFP010000073         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1654942, whole genome shotgun sequence.
ACCESSION   DASSFP010000073 DASSFP010000000
VERSION     DASSFP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654942"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VFH91_01505"
     CDS             <1..1183
                     /locus_tag="VFH91_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6861682.1"
                     /translation="VMINTQDLEKTVDSSKFLRGRDLLCFSHDWSGDPLSKTHLMRIL
                     ARDNRVLWVNSIGYRAPTASKADVSRAFKKLKAATNALSEPEKNIFVLNPLAIPIYGR
                     PRIRELNKSLLRFQVKRAMRTLSFKHPINFVFNPAAAVIAGALDEEQLIYYCVDEYTA
                     FSGVASRSLAELEEQLMRKSDLVVVSADRLFQSKVKINSNTVMIRHGVDFNHFRKALD
                     PETIVPEEIRNLPKPVIGFFGLIADWVDLELIAAVAERFPNGSVVLVGKSTTDTKVLE
                     QEPNVHLLGRKTYESLPGYCKGFDVALMPFRINELTLNANPLKVREYLAAGLPVVSTS
                     IPEVEVLGLCRIGTDSESFIREVETALQHPGPSVEQSELVRSESWDARVDELRGYLSQ
                     L"
     gene            complement(1196..1744)
                     /locus_tag="VFH91_01510"
     CDS             complement(1196..1744)
                     /locus_tag="VFH91_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET6861683.1"
                     /translation="MIKHLVLALVLGVAAFCCSASSVYADSVTLTGGTISTLGGVSSV
                     SLTGQNFSITYSGEIPQGMVTSFGFNTSSAGFGSPLVISNGVSSTFFKGSLAFTDSSL
                     SGSLVAYGSLADLFFNQNALFNVTFTGGGYMTQSNVNGLTQTQFSVVTPTSVPEPSTL
                     VLLLTGGLGLVPLKYRLRNRKH"
     gene            2204..4369
                     /locus_tag="VFH91_01515"
     CDS             2204..4369
                     /locus_tag="VFH91_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HET6861684.1"
                     /translation="MKQVLRKGLKNIVVDEVPDPVTIPNHVLIKPFYSLISSGTETAD
                     IHTDSLVKEVAENPSHLSKIWAVMKQQGVIKTLEEVKAKFSEYAALGYSGAGILVDKH
                     PSVRDFEIGDRVAYGGEGTGHAETVIAGEKLVVKVPDAVSFEQACFTTLGSIALNSIR
                     IAAIGVGDVVAVMGLGLVGQLVAQLVRAQGGVVVAIDMKQDRVDLAKATGADYALPGG
                     ATVPGEINSITNGRGVDCVIVAAASKSPAPVQQAVKISRDRGKIVIVGAVPLDVPRDE
                     MYIKELQLLMSRAYGPGSYDPSYEKEGKDYPFPYVRWTENRNMAEFLRLVANKTVDVD
                     ALISHRFDLGDAAHAYQTIMEPSSNSLAVVLRYPMTSSATTEYAPKHKLEIRKTERKD
                     KLQLALIGAGNLARWEHLPNLKKIPGVTLRAVQSGSGARGKTYGERFGADYSTSAYDE
                     ILSDPNVDVVLIATRHQYHFAQALKAIEAGKHVFIEKPMALTLEECQQLYRAVNGNTA
                     QLTVGFNRRFAKCYKELKRALSGRNGPAIINCRMNSPGLTGSFWAADPKYGGAIVGEG
                     CHFVDLMYWLLESEPVSVVAYTLPTGKSEPIGENNVVASFRYADGSIGNLTYCTVGSK
                     TSGGERVEVYAQGVAAVTEDFKLFELKANSRSKQVSRHPEKGYAEQMTAFIQGIQTGH
                     DADVTVLDGARATIGCLAMLESAHSQEPQSINLSELLAQ"
BASE COUNT         1189 a         1003 c         1227 g         1098 t
ORIGIN      
        1 agtaatgatc aatacacaag acctcgagaa aacggtcgac agctcaaagt tcttacgtgg
       61 ccgtgacctg ctgtgcttca gtcatgactg gagcggagat ccactttcga aaactcatct
      121 tatgaggata ctcgcgcgcg ataaccgtgt cctgtgggtg aattccatcg gatatcgagc
      181 gccaactgct tcgaaggcgg atgtaagccg agcctttaag aaactgaagg cggctacaaa
      241 tgctctcagt gagcccgaaa aaaatatttt tgttttgaat ccactggcta taccaattta
      301 cggtcggccc aggattcgag aactgaacaa gagtctgttg cgttttcaag tgaagcgcgc
      361 catgcggacg ttgtcgttta agcatccgat aaacttcgtc ttcaatcctg ctgccgcggt
      421 catcgcgggt gcgctggatg aagaacaact gatctattac tgcgtagatg aatacaccgc
      481 cttctcggga gtggcatcga ggtccctcgc agaactagaa gagcagttaa tgaggaagtc
      541 ggatttagta gttgtgtcgg cagatcgact atttcaatcg aaggttaaga ttaattcgaa
      601 caccgtcatg atcaggcatg gggtggattt taatcacttt cgtaaagccc tcgatccgga
      661 aaccattgtc ccagaggaaa ttcggaattt acccaaacct gtaatcggat tcttcggact
      721 gattgccgat tgggtcgacc tcgagttgat cgccgctgtt gcagaaagat tccctaatgg
      781 ctccgtcgtg ctggttggca aatcgacgac ggacacaaaa gtacttgagc aagaacccaa
      841 cgttcatctt cttggacgca agacatacga gtcattaccc ggctactgta aaggattcga
      901 tgtcgcgctc atgccattta gaattaacga gctgacatta aacgccaacc ctttaaaggt
      961 gcgagagtat ctggctgcag gtctgcctgt tgtctccact tcaattcctg aagttgaagt
     1021 tcttggtttg tgtcggatag gaaccgactc ggaatcgttt atacgggaag ttgaaaccgc
     1081 attgcagcac cctggtccaa gcgtggaaca aagtgagctc gtgagaagcg agagctggga
     1141 tgcaagagtg gacgaacttc gcggttatct aagtcagttg tagtacccgg tggcgctagt
     1201 gctttcggtt gcgaagtcgg tacttgagtg ggaccaagcc aagaccaccg gtgagcagta
     1261 aaaccaaggt tgagggttcc ggtactgacg tgggtgtaac tactgaaaac tgagtttggg
     1321 ttaagccgtt cacgtttgac tgcgtcatgt agccaccgcc ggtaaaagtc acattgaaga
     1381 gcgcgttttg attgaaaaat aaatcggcaa gggatccgta agcaaccagg gaaccggaaa
     1441 gtgaagagtc agtgaaagct agtgagccct taaaaaaggt cgagctgacg ccatttgaaa
     1501 tcaccagagg tgaaccgaag cccgccgagc tggtgttgaa tccaaaagaa gtaaccatcc
     1561 cttgaggaat ctcgccactg taagtaattg aaaagttctg tccagtaagg ctcacactac
     1621 tcacgccccc aagtgtacta atggttccgc ccgtaagcgt cacgctgtct gcgtatacgc
     1681 tcgatgctga acagcaaaag gcggctacgc cgaggacaag agcgagtaca aggtgtttaa
     1741 tcatgattaa tctcgacaga ttccctttgg gagatttggt gtggtttctt gcgtcggggg
     1801 atgttgccgg cggccccgca agttaaccat ctaaagatac taacaatcgc gggcactttc
     1861 cgctgaattt tgggtagtca gcggtccagc acagaatagc atgcgatcac aaaaagatgc
     1921 tcggagaaaa ttaggccgga tagtctttat tttcttgttt atttgtttct ggaccgcccg
     1981 ggccagacac gtagaaatgg tggtttgaca caattagtcg tgtaacggat attaagcctc
     2041 gttcccagga ggctgtgatc taaaccattt gcaaagccca tcaggctcac aatcaccctc
     2101 cgttcaatca tctggaaatg gtcacgctcc aaagatttag ccgcgaaaat attgatgtag
     2161 aggtgaaaaa tcacgtgcag aaggttgaga accaaaaggc agaatgaaac aggtattgcg
     2221 aaaagggctc aagaatatcg tggttgatga ggttccagac cccgtcacga ttcccaacca
     2281 tgtcctgata aaaccgtttt attctttaat cagtagcgga actgaaactg ccgatatcca
     2341 tactgacagt ttggtaaaag aagttgccga gaatccctcg cacctgagca agatttgggc
     2401 ggtgatgaag caacaaggag taatcaagac gctcgaggaa gtgaaagcaa aattcagcga
     2461 gtacgcagcg ttaggctact caggcgcggg tattttggtt gataagcacc catcagttcg
     2521 tgactttgaa attggtgata gggtcgcgta cggaggagaa ggcacaggtc acgctgagac
     2581 ggtaatagct ggcgagaaac tcgttgtgaa ggtgcccgac gcagtgtcgt ttgagcaggc
     2641 ttgttttacg acgcttggta gtattgcgct gaactcgatc cgaattgccg cgattggtgt
     2701 gggcgatgtg gttgcagtaa tgggcctggg cctggtcggt cagttggtag ctcaactagt
     2761 gcgtgctcag ggcggtgtgg tcgtcgctat tgatatgaaa caagatcggg tcgaccttgc
     2821 gaaagctacc ggcgcagact atgcgctgcc gggtggagcg acggtaccag gagagatcaa
     2881 ttcgataact aatggtcggg gagtcgactg cgtaattgtc gcggcggctt ctaaatcacc
     2941 cgccccggta caacaagccg tgaagattag tcgcgatcga ggcaagatag taatcgtcgg
     3001 cgcagttcca ctggacgttc cccgagacga gatgtacatc aaagagttgc aattgctgat
     3061 gtctcgagct tatgggccag gaagttacga cccttcttat gagaaagaag gcaaagacta
     3121 tccatttcca tatgtccgct ggacagaaaa cagaaacatg gcggagtttc ttcgattagt
     3181 cgcaaacaag actgttgatg ttgatgcttt gattagtcac cggtttgatc tgggagacgc
     3241 cgctcatgct tatcaaacga tcatggagcc gagctcgaac agccttgcag ttgtgcttcg
     3301 ctatccaatg acttcgtctg ctacgactga atatgctccc aaacacaaac tggagattcg
     3361 caagactgaa cgaaaggata aacttcagct tgctttgatt ggcgcaggca atctggctcg
     3421 ttgggaacat cttccaaact tgaagaaaat ccccggcgta acgctccggg ccgtgcaatc
     3481 aggtagcggt gcgcggggaa agacttatgg tgaacggttt ggcgccgact attcaacttc
     3541 ggcttacgac gagattctca gcgatcctaa tgtagacgta gttttgatag cgactcgtca
     3601 tcaataccat tttgcacaag cgttgaaagc aatcgaggcg ggcaagcacg tatttatcga
     3661 aaagccgatg gctttgactc tcgaggaatg ccagcaactc tatcgggccg tcaatggcaa
     3721 tacagctcaa ctaactgtcg gctttaatcg tcgcttcgca aagtgctaca aagagctaaa
     3781 acgagcgttg agcgggcgaa atggaccagc aattattaat tgccggatga actctccggg
     3841 tttaaccggc tctttttggg cggctgatcc gaaatatggc ggtgccatag ttggtgaagg
     3901 gtgtcacttt gtggatctga tgtactggtt actggaatct gagccggtta gtgttgtcgc
     3961 gtatactctg cctactggaa agagcgagcc gattggcgaa aacaacgtag tggcgagttt
     4021 tcgatatgcc gacggctcaa ttggaaatct aacgtactgt acggttggga gtaagacttc
     4081 gggtggtgaa cgagttgaag tctatgctca aggagtagct gcagtcacag aggactttaa
     4141 acttttcgaa ctgaaggcaa atagtcgcag caagcaggtc agccgtcatc ccgagaaagg
     4201 atacgctgaa cagatgaccg cttttattca aggaatacaa accggtcatg atgccgacgt
     4261 aactgttttg gacggggctc gagcgacgat tggctgtctg gcgatgttag agtcagctca
     4321 tagtcaggag ccgcagtcaa ttaacctgtc ggaattattg gcccagtaaa ctttgtggtg
     4381 ttgcaaggtg ggtaagttct atgaagtacg agtcgcatcg taagtggtta ggtcttcaga
     4441 aggttctttg tctaatcatt atttttgtgt gcggcgtgtc gagcgcttgc gcacagcaac
     4501 gtgcccgcac ccaaacc
//
LOCUS       DASSFP010000074         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1803723, whole genome shotgun sequence.
ACCESSION   DASSFP010000074 DASSFP010000000
VERSION     DASSFP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803723"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..612
                     /gene="ytxJ"
                     /locus_tag="VFH91_01520"
     CDS             280..612
                     /gene="ytxJ"
                     /locus_tag="VFH91_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HET6861685.1"
                     /translation="MGNKFLRIDDRKRLEDVISDSTTKPVVIFKHSNSCPISSRAYRE
                     MEQLDGQVNLVEVQAARDISRELADLTGVRHETPQVIVLKNGRAVWNASHFDVKAGAV
                     HEAVEANS"
     gene            614..937
                     /locus_tag="VFH91_01525"
     CDS             614..937
                     /locus_tag="VFH91_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET6861686.1"
                     /translation="MVDVSGLQIEPREIKQEDDFNLRITWGDGRECRFNAAALRRACP
                     CAQCVNEWTGERMLKPNTIADELEIKDLSVVGRYALNFQWSDGHQTGIYSFRYLRELC
                     EGQEM"
     gene            953..1225
                     /locus_tag="VFH91_01530"
     CDS             953..1225
                     /locus_tag="VFH91_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861687.1"
                     /translation="MNERDFFNEKEEIKKATFSCPHCRERAEYDVRWLKRTRKPQLPR
                     GANEQDRARFQKSRDYLVRIDDMLACKNMRCRRRFEIPMSQSVVFI"
     gene            1710..2393
                     /locus_tag="VFH91_01535"
     CDS             1710..2393
                     /locus_tag="VFH91_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6861688.1"
                     /translation="MIETQQFAGATKASTSFLTPLERRLASRVLPIIPSWLETYHLTM
                     LTLVWSAGIIFFSYLAATNIRWLWMVSLMILMQYVTDHYDGKIGKYRGTGLVRWGYYM
                     DHFLDYIFLCSIIVGYGFILPERSRYQILIMLAIFAGYEVSTFLAFAATDRLKIAYLK
                     FGPTEFRLALIIINALLVAYGTRKMVSGLKYVNIGAAVGLATMVFRTHRLIWEIDMKQ
                     KRLKSGQNN"
BASE COUNT          651 a          557 c          618 g          640 t
ORIGIN      
        1 ttaataagaa gctcgtgagg ctattcagaa gagtgcctgg ctcagaggat agccaacgct
       61 ttctcggtca ggttcggcaa gatattctct ttgtgctatt cggtcaatag gaaagttgtt
      121 tttggttttg gtttggccct aggcctttga tcttcggcgg tgttcccaaa atcgtaaacg
      181 gacaaatggc gaagtccaaa gatcaaaggc caaagcctca gtttgtccca accctttcag
      241 taagctaata tcgaagcatc acaagaggga tagcaaaaca tgggtaacaa gtttttaaga
      301 atagacgatc gcaaaagact tgaagatgta atttccgact cgacaacgaa accggttgtt
      361 atcttcaagc acagcaactc gtgtccgatc agctcaagag cttatcgtga aatggaacag
      421 ttggacggac aggtaaattt ggtggaggtg caggcggctc gagacatttc gcgcgagctt
      481 gcggacctga ctggagtacg tcatgaaacg cctcaagtaa tagtgctgaa aaatggtagg
      541 gccgtttgga atgcctcgca tttcgatgta aaagcgggtg cggtccatga ggcggtagaa
      601 gcaaacagtt aatatggttg acgtcagcgg attgcagata gaacctcggg aaatcaaaca
      661 ggaagatgat ttcaacctga ggatcacatg gggcgatggc cgcgagtgcc gattcaacgc
      721 ggcggcgctc agacgtgctt gtccgtgtgc ccaatgcgtc aatgaatgga ctggcgagcg
      781 gatgttaaaa ccaaatacca ttgccgacga actcgagatt aaggatctga gtgtggtcgg
      841 tagatatgct ttgaacttcc agtggagtga tggacatcaa accggcatct atagtttccg
      901 ctatcttcgc gaactctgcg aagggcagga aatgtgaagt ctgagacaag ttatgaatga
      961 gcgagatttt tttaacgaga aagaagaaat aaagaaggcg acgtttagct gtcctcactg
     1021 tcgcgaacga gccgaatatg atgtgcgttg gttaaagcgc actcgcaaac cgcaactacc
     1081 gcgtggagca aacgaacagg atagggcgcg atttcagaaa tcgagggatt atctcgtccg
     1141 gatcgatgac atgctcgcct gtaaaaatat gcgttgtcgc agacgcttcg aaattcccat
     1201 gtcgcagtcg gtggtgttta tttgatcttc aagcgctgct gacttccagg aattccacca
     1261 acggcagttg acggttcgtt aaagtccaac ctacgaagaa gcccacggca cgacttccag
     1321 gaattccacc aactgcccgt cgggtgatat ttgagttttt acacagtctc tttccgccgc
     1381 gtggtccatc aacttcgacc taacccaaca aacctcctga tcgtcttgga tatcagcatt
     1441 gctgggtcta cctccgttct atgagcacag cttttgcact gagatcgtcc gccagttaga
     1501 aaacgacaag atgaatttag aagcaccagg agccaaactt tctcgaaaag ggccataaac
     1561 aatgaaacat tcgcatccga cattgtttgg tgaggccctg tttgagttca tattcgctct
     1621 tctcgaagtc ttaataactt tgataatcga gctctaacgg tgtaaagctc ccgacactag
     1681 tcggccaaat ctgacacttt tgaaatcaca tgattgagac gcaacaattc gccggggcaa
     1741 cgaaagcctc aacatcgttt ctgacgccac ttgaaagacg tttagcctca agagtgttgc
     1801 caataattcc gtcatggctg gagacttacc acctgactat gctcacgctc gtgtggtcgg
     1861 ctggaattat tttcttctcg tacctggcag cgacgaacat acgctggcta tggatggttt
     1921 cgttgatgat cctcatgcag tacgtaaccg atcattacga cggcaagatt ggaaagtacc
     1981 ggggcacggg tttagttcgg tggggctact atatggatca ctttctggac tacatctttt
     2041 tgtgctcgat cattgtcggt tacggtttta tcttgcccga acgttcgcgc taccagatct
     2101 taataatgct tgcgatcttt gccggatatg aggtaagcac ctttctggct tttgccgcga
     2161 ctgatcggtt aaagattgcg tatctgaagt ttggacctac ggagttccgc ctggctttga
     2221 tcatcattaa cgcactcctt gtagcttatg gcacccgcaa aatggtttcc ggtttgaaat
     2281 acgtcaacat tggcgcagca gtcggtctgg caaccatggt tttcaggacc cataggttga
     2341 tttgggagat tgacatgaag caaaaacgat tgaaatcagg tcaaaataac taatcctgtc
     2401 ttgcttgctg tcagtgttct cctttccgct gtacggtttg cgatcaaaat caaccatatc
     2461 ggagaa
//
LOCUS       DASSFP010000075         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2099312, whole genome shotgun sequence.
ACCESSION   DASSFP010000075 DASSFP010000000
VERSION     DASSFP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099312"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="VFH91_01540"
     CDS             <1..1872
                     /locus_tag="VFH91_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET6861689.1"
                     /translation="ARTIEEVAKIVGTAGTRFSSHNWRSVASGVSGGWTVARALFMLN
                     ALLGAVATEGGVYPNAWNKFVPRPIYTPPHPNMWNETTWPREYPLSMNEMSFLLPHFL
                     KDGRAKLDVYFTRVYNPVWTNPDGFSWIEMLTAPDLVGCYVALTPTWNETAYYADYIL
                     PMGVGSERHDLHSYETHDSQWLGFRQPVMKALKQREGSTVSDTREVNPGEVWEENEFW
                     LELTWRIDPDGSLGIRQYAESKSNPGTRLGIDEYYEWIFNNSVPGLPEKAAAEGLTPL
                     QFMRRYGAFEVKEKIGAIYDQAITPEELDDAREDYFGRVYTRAAKPAGPNIVPLPLPD
                     GDDEGRRLVGIRVDNEIKRGFPTPSGKLEFYSTTLRNWGWPELAIPTYFKSHVHPDNL
                     EKDQTILISTFRLPVQIHTRSANSKWLDEIAHTNPLWIHPSHADKINVYTGDLVRVET
                     EIGYYVVPAWVTEGIRPGIVACSHHMGRWKLTDEGQRQLMATVKLKHKDNKWALRREK
                     GIEPYESEDADTLRIWWKDAGVHQNMTFPVHPDPISGMHCWHQAVRVRPALAEDRPGD
                     VFVDTEKSREVYKKWLAMTRPADTYSPDRTRRPYWMLRPLKPGREFYKLPEEPGVRS"
     gene            2062..3471
                     /locus_tag="VFH91_01545"
     CDS             2062..3471
                     /locus_tag="VFH91_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6861690.1"
                     /translation="MYFKQFYLGCLAHASYLLGSNGQAAVIDPQRDVDEYLAEAEANG
                     LEIKYVIETHLHADFVSGHQEIAARTGAQIVFGESANAEFEHRGVKDGHEIKLGEVIL
                     RFLETPGHTPEGISILVVDTTVSNQPQKVLTGDTLFIGDVGRPDLAGGKGFTPQQMAG
                     MLYDSLHEKLLKLPDEVEVYPAHGAGSMCGKNMSKETSSTIGEQRKFNYALKPMSREE
                     FVTMMTADLPEAPAYFPKDAEINRSGARDLSELSPPKPLGPAEVARLRDAGHFLLDTR
                     SYSDFGAAHVPGSVNIGLGGQFAMWAGSLIPFTAEILIIADTNAQVDEAVVRLARVGI
                     EKVRGYLDGGLESWTNAGLPVSTIKQVSVEQLSELLKNQSLRQVIDVRRPMEYQSGHV
                     PGAFTSPLAALGQDVNRLSVNRDEPTAVICAGGYRSSAAASLLEAQGFNNLLNVTGGT
                     SAWVNAGFPVETTSESKSN"
BASE COUNT          935 a          892 c          966 g          869 t
ORIGIN      
        1 gcacgcacaa tagaagaggt ggcaaagatt gttgggaccg ctggaactcg cttctcaagc
       61 cataactggc gcagcgtcgc ttccggcgtt agcggaggtt ggaccgttgc ccgtgcactc
      121 ttcatgctta atgcattgtt aggcgccgta gcaaccgaag gcggcgtcta tcccaacgca
      181 tggaacaagt ttgtaccaag gccaatctac actcctcctc acccaaatat gtggaacgaa
      241 accacctggc cgcgcgagta tccgctttca atgaatgaga tgtcgtttct gttgccgcac
      301 ttcttaaaag atggacgcgc aaaactcgac gtctacttta ctcgcgttta caaccctgta
      361 tggaccaatc ccgacgggtt ctcgtggatt gaaatgctga ccgcaccaga tctggtcggt
      421 tgttatgtag cgctcacgcc aacctggaat gagaccgcgt actatgcgga ctacatcctg
      481 cctatgggcg ttggatccga acgtcacgat ctgcactcct acgaaactca cgattcgcaa
      541 tggctgggtt ttcgccaacc cgtgatgaaa gcgttaaagc aacgtgaggg ctcgacagtc
      601 tctgatacgc gcgaagttaa tccgggcgag gtttgggaag agaatgagtt ttggttggaa
      661 ctaacctgga gaatcgatcc ggatggcagt ctgggtattc gtcagtacgc agagtcaaag
      721 tcgaaccctg gcacgcgact cggaattgat gagtactatg aatggatctt caacaactct
      781 gtgcccggac ttcctgagaa agcagccgcg gaaggtttga ccccactgca attcatgagg
      841 cgctatggtg cgttcgaagt aaaagaaaaa attggcgcga tctatgatca agcgataaca
      901 cccgaagagc tggatgacgc tcgcgaagat tactttggaa gagtctatac gcgcgcagcg
      961 aaacctgctg gcccgaacat cgttccactg cctctgccgg acggggatga cgaaggacgc
     1021 cggttggttg ggattagagt cgacaatgag atcaaacgcg gattcccaac gccgagtgga
     1081 aagcttgaat tctattcgac cactcttcga aactggggtt ggcccgaact cgcgatacca
     1141 acctatttta agagtcatgt tcatccagac aatctggaaa aagatcagac aattctaatc
     1201 tcaacttttc gacttccggt tcagattcac acgcgcagtg ctaattcaaa atggctcgat
     1261 gagattgctc acacaaatcc tctgtggatt catccgtctc atgctgacaa gataaatgtc
     1321 tacacgggag atcttgttag agttgaaaca gaaattggct attacgttgt tccagcctgg
     1381 gtgaccgaag gaattcgacc cgggatagtg gcttgcagtc atcacatggg tcgctggaaa
     1441 ctgaccgatg aaggtcagcg acaattgatg gctaccgtta agttaaagca taaagacaac
     1501 aagtgggcat tacgacgtga gaaagggatc gagccctacg aatcggaaga cgctgacaca
     1561 ctgcgaattt ggtggaagga tgctggagtt caccaaaaca tgacgttccc ggtccatccc
     1621 gatcctattt caggaatgca ttgctggcac caggcggtgc gagtgcgtcc agcacttgcg
     1681 gaagatcgcc cgggtgacgt cttcgtggac actgaaaaat ctcgagaggt ctataagaag
     1741 tggctcgcga tgactcgtcc tgccgacaca tattctcctg accgaacgcg acgaccgtat
     1801 tggatgctgc gaccgttgaa accaggtcgc gagttctata aattgccgga agaacctggt
     1861 gtgaggagtt gaggaagatc atttgtcata tatcattttt catttctcat ttttcatttg
     1921 tttctcgagc acctgagtag aattttgaga cgtttaggag ttaaatgggt gtgtcaaaaa
     1981 caatgccaaa tgggaaatgg gaaatgatat atgacaaatg gttgaaaaac ctttgactag
     2041 tctcaaacca cttatcacac aatgtacttc aaacaattct atctcggctg tctggcgcat
     2101 gcttcctact tgctcggatc aaatggtcag gctgcggtca tcgatccgca gcgcgatgta
     2161 gacgaatatc tcgcagaggc tgaagcgaac ggtctcgaga tcaagtacgt catagaaact
     2221 cacctacacg ccgatttcgt tagcggtcat caggaaattg ccgctcgtac tggggcccaa
     2281 atcgttttcg gcgagagtgc aaacgccgag tttgagcacc gcggcgtcaa agatggtcat
     2341 gagataaaac ttggagaggt catcctcaga tttcttgaaa cgcccgggca tactccggaa
     2401 ggtatttcca tacttgtagt ggatacgaca gtttcaaacc aaccgcagaa ggtcttaacc
     2461 ggcgacacgc tgttcatcgg tgatgtcggc cgtccggatc tcgccggcgg caaaggtttc
     2521 acgcctcaac aaatggcggg gatgctttac gacagcttgc atgaaaaact tttgaagctt
     2581 cctgatgagg ttgaggttta tcccgcgcat ggagctggtt cgatgtgtgg gaaaaacatg
     2641 tcgaaagaga cctcgtcaac tatcggagaa caacgtaaat tcaattacgc cttgaaaccg
     2701 atgagccggg aagagtttgt cacgatgatg acagcggatc tccctgaagc tccggcttac
     2761 tttcctaagg acgccgagat taaccgctcg ggggcacggg acctttccga actttcaccg
     2821 ccaaaaccac ttgggccggc cgaggtcgcg cgactgcgtg atgccggaca ctttctactt
     2881 gatactagat cgtattctga tttcggcgct gcacatgtcc cgggctccgt aaatatagga
     2941 ttggggggcc agttcgccat gtgggccgga agtcttattc cttttactgc agagattctg
     3001 atcatcgccg acaccaacgc gcaggtcgat gaagcagtag ttcgattggc gcgggtcggt
     3061 atcgagaaag tgcgaggcta tctcgacggc ggccttgaaa gttggacgaa cgctggtttg
     3121 ccggtcagca caatcaagca agtctctgtc gagcaattga gtgaactgct taaaaaccaa
     3181 agcttgcgac aagttatcga tgtgcgacgg ccgatggaat atcaaagcgg tcacgtcccc
     3241 ggcgctttta cttcgccgct tgctgcttta ggtcaggacg tcaaccggtt gagcgtaaat
     3301 cgtgatgaac cgacggcggt tatctgtgcc ggaggctatc gatctagtgc ggctgcgagt
     3361 ttgctggaag cgcagggctt caataatctc ctgaatgtca ccggcggaac cagcgcgtgg
     3421 gtaaatgccg ggttccccgt agagacaact tcggagtcga aatccaactg aaccgaaact
     3481 ggtcccagtt tgaaaatttc cggcagtccc ctccctggcc cataaattgt caatcctgtg
     3541 aatcttgtct cacgtgcttg tcgttattag ggttgttatt tgacgataag tcaaaatgcc
     3601 gtttagcatc gcggcaatct ttaaaaacga tcaactagga gtgagaccat aaatccagtc
     3661 ac
//
LOCUS       DASSFP010000076         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1655932, whole genome shotgun sequence.
ACCESSION   DASSFP010000076 DASSFP010000000
VERSION     DASSFP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655932"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFH91_01550"
     CDS             <1..893
                     /locus_tag="VFH91_01550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6861691.1"
                     /translation="SDFNDLFMVHATIMAVLFATSFFTLRFGVAGPSPGTSVMSVALL
                     LLTLGFLHYLPLFGARKGLFGVIVPPGYLQYTSICDMILEIVLGFGTMMLLMEAIHRE
                     AEKANRELTEARDKLELLARMDPLTEALNRHAFHSLLSRSERDDHAETFGCVAVLDID
                     NLKPINDTHGHAAGDKAIRAVARAMRSLIRADDMLFRWGGDEFLVLMFNLSETEVSRR
                     IATLNGILERNCEHWTGLPIKVTVSYGVAGFCSMRGLAAGIEAADQAMYAQREKVRGS
                     TKFKGPSMLPLNPTVLLDVH"
     gene            complement(906..1928)
                     /locus_tag="VFH91_01555"
     CDS             complement(906..1928)
                     /locus_tag="VFH91_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET6861692.1"
                     /translation="MTTPTRPVQVDRDRSVAGLLEKMEGTGFGGRQLAEAHRIWLDML
                     GNNTTIMVAGSGALIPAGMRRLLAYVIKNRFVDVLVLSGSMLFHDLHETLGRYHFQAH
                     PSMTDAELESAQISRMWDLLASDEEYREAKEWIGGFTNQLDQTRSYSPREFLHLLGRE
                     LAEIASEDGVLTSAYKSRVPIFCPAIADSGLAVGIAGARFEKKNNFMFDIIQDVLDMM
                     NVMAHARVSGIINLGGGTPKSFIQQATVSSFLQKEHHHGHKYAIQISADGPHPGGRSN
                     GVSFDEGLVYGKLARGAHTAYVPCDATIALPILITSLSQTAAKYLKGRKRPNFTFGRE
                     LLVEAP"
     gene            complement(2019..3956)
                     /gene="speA"
                     /locus_tag="VFH91_01560"
     CDS             complement(2019..3956)
                     /gene="speA"
                     /locus_tag="VFH91_01560"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET6861693.1"
                     /translation="MTTAIEQTLEIYGIENWGAGYFDVNRKGNLIVHPVEGDPRSADL
                     KEIIDNLAKRGITTPVLLRFPQLVAAQVRKLQRAFSRSIREYEYQGAHMCVYPMKVNQ
                     QRAVVEEYLREGSRYDFGLEAGSKAELYAALALEQPAESLLVLNGFKDIEFIELAFAG
                     ARCGKKVVIVIEKLNELDHVLTVAEQHEGPMPMIGMRVKLYSKGSGRWEKSGGEAAKF
                     GLTTTELLEVIRRLEEAGRMDMLKLLHFHIGSQLTDIKRIKNAMKEAARVYAKVYQMK
                     VPIELLDVGGGMAVDYDGSKTAFDSSANYTAQEFANDVIYTIKQVCDDENVPHPTIIQ
                     ESGRFLAAYHAILITNVLDEIETVVEDITPIEIDEDDPQVVTELGELRESITVKNYRE
                     YYHDALEHREELFTLFNLGLISLEDRAKGEVLFWDVCERADKYAQQAKYVSEEFGELR
                     RLLCAKYLTNFSVFRSVPDHWALDQLFPIVPIHGLNKPPTEYTTLCDITCDSDGIVDK
                     FVDLHDVKQVLELHALSADEPYYLAFLLVGAYQEVMGNNHNLFGAPHEAHIHIDEEGF
                     LIKKVIKGTTVGAAAERARYERSFLHDGFRRLINRRVKEGELSEAEGAQLAQFYESRY
                     DAYTYLAVNGIKPRSRRKTDY"
BASE COUNT         1047 a         1132 c         1107 g         1144 t
ORIGIN      
        1 cttccgactt taatgattta ttcatggtcc atgcgacgat tatggcagtc ctttttgcca
       61 catcgttctt cactcttcgt tttggagtgg ctggtccaag ccctggtacc agtgttatgt
      121 cggtggcgct gttattgctc acgctcggct ttctccacta cctgccactg ttcggagcac
      181 gaaaagggct tttcggtgta attgttcccc ccggctatct gcaatacacc tcgatctgcg
      241 acatgatcct cgaaatcgtc cttggtttcg gtacgatgat gttgttgatg gaggctattc
      301 accgcgaggc ggaaaaggct aacagagaac tgacagaagc gcgcgataaa cttgaactcc
      361 tcgctcggat ggacccgctg accgaagcgc tgaaccgtca tgcatttcat tctctcctca
      421 gtcgttcaga acgagatgat cacgccgaga ctttcggttg cgttgccgtg ctcgacatcg
      481 acaatctgaa gcccataaat gatacgcatg gacatgctgc cggcgataaa gcaatacgtg
      541 ccgtcgctcg agcaatgcgc tcattgatca gagctgacga catgctgttc cgttggggtg
      601 gggacgagtt tctcgttctg atgtttaatc tttcagagac cgaagtcagc agaagaattg
      661 cgaccctgaa tggtatcctt gaacgtaatt gcgagcattg gaccggtttg cctataaaag
      721 tgactgtctc ttatggcgtt gctggattct gttctatgag gggactcgca gcgggcattg
      781 aagcggcaga tcaggccatg tacgctcagc gagaaaaggt gcgcggatca acgaagttca
      841 aagggccctc aatgctgccg cttaacccga cggtcttgct ggatgttcat taaaaactga
      901 aattatcagg gcgcttcgac gagcagttcg cgaccgaatg tgaagttagg acgctttcga
      961 cccttcaagt acttggcagc tgtttgcgac aacgatgtga tcaagatcgg aagtgcgatt
     1021 gttgcgtcgc acggaacata cgccgtgtga gcgccacgcg ccagcttgcc atagaccaaa
     1081 ccttcatcga aagacactcc attggaccgt cctcccggat gaggtccatc cgctgaaatc
     1141 tggatagcgt atttgtgacc gtggtggtgt tccttttgca gaaatgagga aacggtagcc
     1201 tgttgaatga agctcttggg tgtgcctcca ccaaggttga tgatgccgga gacacgtgca
     1261 tgggccatga cgttcatcat gtcgaggaca tcttgaatga tgtcgaacat gaagttgttt
     1321 ttcttttcga atctcgcgcc tgctattccc accgccagcc cactatctgc tatcgccggg
     1381 caaaaaatcg gcacgcgaga tttgtaagct gaggtcagaa cgccatcttc cgacgcaatt
     1441 tcggcgagtt cccgacctaa caaatgcaga aactctcgcg gtgaataaga tctggtttgg
     1501 tctagctggt tagtaaatcc accaatccac tctttcgctt cacgatattc ctcatcgctg
     1561 gctagcaggt cccacatccg gctgatctgt gccgactcaa gttcagcatc cgtcatgctg
     1621 ggatgggcct gaaaatgata ccggccgagt gtttcatgta agtcatgaaa taacatcgac
     1681 cctgacagca ccaaaacatc gacaaagcga ttcttgataa cgtacgccag taagcgccgc
     1741 attccagcag gaatcaaggc gccggagccg gcgaccataa tggtggtgtt gttgccaagc
     1801 atatcgagcc agatgcggtg agcctcggca agttgtcgac caccgaatcc agtaccttcc
     1861 atcttttcca atagccccgc taccgaacga tctcgatcta cctgaaccgg ccgtgtgggg
     1921 gtggtcaagt acttcgatgc ttttggtttg cgctgagtga ccatgcaggt tatcaacctc
     1981 tcgaacaggg ttccctggta tgaatctcaa ttacatcttt agtaatccgt ttttcgtctt
     2041 gagcgtggct tgattccgtt aactgccagg tatgtataag cgtcgtagcg cgattcatag
     2101 aattgcgcta actgagctcc ttctgcctcg ctcaattcgc cctctttgac gcgccggtta
     2161 attagccggc gaaaaccatc gtgcaagaaa ctccgttcgt aacgcgcccg ttcagcagcg
     2221 gcgcccaccg tagttccctt tattactttc ttgatcaaaa agccttcttc atcgatgtgg
     2281 atgtgagcct catgtggcgc tccgaataga ttgtgattgt tacccattac ttcctgataa
     2341 gcgcctacta acagaaacgc caggtaataa ggttcatcag cactcaacgc gtgcagttcg
     2401 agaacctgtt tcacatcgtg aaggtcgaca aacttgtcaa cgatgccgtc tgaatcgcag
     2461 gtaatgtcac agagagtcgt gtattcagtt ggcggtttgt taagaccgtg aatcggaacg
     2521 atcggaaata gttggtccag ggcccagtga tccggaacag aacgaaaaac ggagaagtta
     2581 gtcagatatt tagcgcatag caatcggcgc agctcaccaa attcctcaga tacgtatttg
     2641 gcttgctgtg cgtacttatc ggcgcgctca caaacgtccc agaacaagac ttcgcccttc
     2701 gcgcgatcct cgagggagat tagaccgagg ttgaatagtg tgaacaactc ttcgcgatgt
     2761 tcgagcgcat cgtgataata ctcacgataa ttcttgacgg taatcgattc ccgcaactcg
     2821 ccgagttcag tcactacttg cggatcgtct tcgtcgatct caatcggcgt tatgtcctcg
     2881 acgaccgttt caatctcgtc caatacattt gtaattagaa tcgcgtggta ggccgcaaga
     2941 aaacggcccg actcctgaat aatggtcggg tgcggaacat tctcgtcatc acaaacttgc
     3001 ttgatggtat agatgacgtc gttggcgaat tcctgggccg tatagtttgc cgaagagtcg
     3061 aaggcagttt ttgaaccatc gtaatcgacg gccattccgc cacctacatc gaggagctcg
     3121 ataggtacct tcatctggta caccttcgca taaacgcgag ctgcctcttt catggcattc
     3181 ttaattcgtt tgatatcagt aagctgagag ccgatgtgga agtgcagcag ctttagcata
     3241 tccattcgac cggcttcttc cagccggcga ataacttcca ggagttctgt ggtcgtcagg
     3301 ccgaacttcg cagcttcgcc gcccgacttt tcccagcgtc cagaaccttt tgagtagagt
     3361 ttgacccgca tgccaatcat tggcattggg ccctcgtgtt gttcagctac ggtcagcaca
     3421 tgatcgagct cgttcagctt ttcgataacg atgacgacct ttttgccgca acgagcaccc
     3481 gcaaaagcca attcaataaa ctcgatatct ttaaatccgt taagaacgag caggctttct
     3541 gcaggttgtt caagggccag cgccgcgtaa agttcagctt tggagccagc ttccaagccg
     3601 aagtcatagc gagaaccctc gcgcaagtat tcctccacca ccgcgcgttg ttgattaacc
     3661 ttcatagggt aaacacacat atgtgcaccc tggtattcat attcgcgtat ggaccggctg
     3721 aaagctcttt gaagttttcg gacctgagct gctacgagtt gggggaaacg aagaaggacc
     3781 ggcgtggtga ttcctcgttt ggccaggtta tcgataatct ctttgaggtc cgctgagcgg
     3841 gggtcgccct caacagggtg gacgattaga tttcccttcc ggttaacgtc aaagtatcca
     3901 gcaccccagt tttcaattcc gtaaatttcg agcgtctgct cgattgccgt ggtcaattgg
     3961 cggtctcctg cctgataacg ttagatatgg attcctttgc ccaaaacgcg gtaatacacc
     4021 ttctcgatac gagttgcgga gttgtgctgg gtggaacgct ttagtggccg aatgtaacag
     4081 aaaacgttag gagggtctat aacttttttg tttcaaaaag ggcctcaggt tgacgtaaac
     4141 gctcagaaac tttaaggttt ttggcccatt ttcaattgtg gctggtccta caccgtcttg
     4201 ccgattaaaa accgcgagcc acgtgatgcc gagaaatttt gtaataccca ccacaaaaag
     4261 cctcgaacca aacaaaaagt tcggtactgg ttcagtttaa tttctcggcg tttctctgcg
     4321 tgatctccgc gttctctgcg tcttggctgt gtagctctga taaggaagtc cggacaagac
     4381 gcagagtacg cggagacacg cagagaacgc aaaggaaagc agcctgaacc
//
LOCUS       DASSFP010000077         4800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1804666, whole genome shotgun sequence.
ACCESSION   DASSFP010000077 DASSFP010000000
VERSION     DASSFP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804666"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFH91_01565"
     CDS             complement(<1..926)
                     /locus_tag="VFH91_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET6861694.1"
                     /translation="MPASKRSVTFSQLRVGIFVLLAIGILVFLVLNASGDINPFSKKL
                     HLKARFSDANGLREGSEVRLAGVRVGKVESIRLLPPSDVPNAPRVEATMTIDSTIDGQ
                     PANVRIRSDSTAQQGSPSLLGNEMLINITPGTAVGQPVSEGAILPSSSSNTVNDFATS
                     GTDLAQRLSKLSDEISGIVRDVKDGKGTVGRLFNDEALYNNLNATIRETEDVMRQVRS
                     GNGSAGRFINDPALYENANQIAIQLKTITDDLRAGRGSAGKFLTDDELYNRINRTADR
                     IDRSVEQLNLIVADINAGHGTLGKLIRDEQLY"
     gene            complement(981..1889)
                     /locus_tag="VFH91_01570"
     CDS             complement(981..1889)
                     /locus_tag="VFH91_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6861695.1"
                     /translation="MLNDPMSSESIHITSQSVSENAAESTFHEVDDRERAIPAIEFRN
                     VLLAFDERVILDHLSFKVMKGETKIILGGSGGGKSTIIKLVLGLLKPDTGRVLVDGED
                     ITDYSEDQMMKVRKKIGMVFQEGALFDSLSVYENVAYRLHEQGVPEDEVEPEVRRMLR
                     FVNLEDAIDKMPIELSGGMRRRVGIARALIGDPKIVLFDEPTAGLDPPTARTICELAM
                     KLRDLEDVSSIFVTHEMNNLQYICSEYAVVDDEGNVVFEREGERLCLINSKVMMMKQG
                     KIIFSGTDESLRKAEDPYIQKFLRGH"
     gene            complement(1890..2654)
                     /locus_tag="VFH91_01575"
     CDS             complement(1890..2654)
                     /locus_tag="VFH91_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6861696.1"
                     /translation="MNFATRAVWELQETTLLAARAFRGIFKRPRYIPETIAQMDAIGV
                     GSLTIIVLTGFFTGGVLTVQTFPTLKAYGAQSQTGYLVAISLVRELGPVLCALMVTGR
                     VGSAISAELGSMVVSQQIDAMRALGTDPIRKLVAPRLFALLVSLPLLTVLADVMGIFG
                     GWVTAISLYSISSSVFVSAVRDGITTDDLIGGVVKPIAFSLIIAAIACRQGLSTEGGT
                     VGVGRSTTRAVVMASIVVIIADFFLAKALQVILGTS"
     gene            complement(2965..3453)
                     /locus_tag="VFH91_01580"
     CDS             complement(2965..3453)
                     /locus_tag="VFH91_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET6861697.1"
                     /translation="MLLNHKSLLLTLVLGFVLSASAVAFSQEPQAGQQAPDLIEQLRL
                     TPEQRQKIRAIREQSKDERMAVNQRLRESNLALEQALDSDNLDESVVEQRITEVNAAQ
                     GAQLRMRIQNEVQIRRVLTPDQIATWRLLRQRTRDVLRSLPNPRRRAGTMGLPRNVAP
                     RR"
     gene            complement(3468..4136)
                     /locus_tag="VFH91_01585"
     CDS             complement(3468..4136)
                     /locus_tag="VFH91_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861698.1"
                     /translation="MNQDFLAHACNRQDELISVLYGEASAEDIRQFQSHLRQCASCNE
                     QLEAFSGVRTSVRDLRDQALVGFQSASISRKVERKSARAALKAFFELSPAWMKFGTAL
                     ATAVLCVFGFIVFRRMERPVASPPVIAARTKVYSEAELKDAVAKAVTEATIAAKQAGI
                     ERSSTPSQESTGRMSVNRNPSPSPKNRRPLSRDERQQLASDLRLVTRPDDDGLQLLSD
                     RINQ"
     gene            complement(4155..4733)
                     /locus_tag="VFH91_01590"
     CDS             complement(4155..4733)
                     /locus_tag="VFH91_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6861699.1"
                     /translation="MDTLTNDETIVERALAGDPEAFGEIVRRWERRIFALSYGMLGRE
                     DEARDATQETFLAAFRNLRGFRGEAKVSSWLHRIAVNQCITRQRRAKVRSESALDDEQ
                     EKQGADFAAPINYSPSHVIESQQETDAVRRAIQSLPVELRQVVVMKEFEELTFREISE
                     ALDIPLSTVKSRLYTALKQLQMRLQKFANDAT"
BASE COUNT         1127 a         1250 c         1200 g         1223 t
ORIGIN      
        1 ttatatagct gctcgtcccg aatgagtttg ccgagcgtac catgcccggc attgatgtcc
       61 gctacaatca agttgagttg ttcaaccgaa cgatcgatcc ggtccgctgt gcggttgatg
      121 cggttataca actcgtcgtc cgtcaggaac ttgcctgctg aacctcgtcc tgctcggaga
      181 tcatcggtga ttgtcttaag ttggatggcg atctgattgg cgttttcata aagtgccgga
      241 tcgttgataa agcgtccagc cgaaccgttg ccggatctga cctgccgcat cacatcttcg
      301 gtctcacgaa tagtcgcatt caggttgttg tatagcgctt catcgttgaa cagccgtcca
      361 acggtccctt tcccgtcctt tacgtcccgt acgattccac taatctcatc cgacagcttg
      421 ctcaatcgct gggccagatc agttccggat gtggcaaagt cgttgaccgt attcgaactc
      481 gaggatggca gtattgcgcc ctcacttact ggttggccca ctgcggttcc aggagtgata
      541 ttaatcaaca tttcatttcc aagaaggctg ggtgatcctt gttgcgcggt tgagtcggaa
      601 cgaatcctta cattggcagg ttgtccgtcg atcgttgaat cgatagtcat ggtcgcctcg
      661 acccggggcg catttggaac gtcagaaggc gggagcaatc gtatactttc gaccttgcct
      721 acgcgcacac cggccagccg cacctctgat ccctcacgca agccattggc gtccgaaaaa
      781 cgcgccttca ggtgtagctt tttgctgaaa ggattgatat cgccggaggc gttgagtact
      841 aaaaagacca gaatgccaat cgccagaagt acgaaaatac caacacgcaa ttggctgaat
      901 gtaacactcc gttttgatgc aggcatggcg tgcagttggg gatggtggaa atgaacttaa
      961 agctcgtccc cagtcctttc tcaatgtccg cgcaggaact tctggatgta agggtcctcg
     1021 gccttgcgca atgattcgtc agttccactg aaaataattt ttccctgttt catcatcata
     1081 actttgctat ttattaagca gagccgctcg ccctctcgtt caaacactac attaccttcg
     1141 tcgtcaacca ccgcatactc ggaacagata tattgcaagt tgttcatttc gtgcgtcacg
     1201 aaaatcgacg agacatcttc gagatctcgt aacttcatcg ccaattcgca gatcgttctg
     1261 gcggtgggag gatcgagacc agcggtcggc tcgtcaaaaa gtacgatttt gggatcgcca
     1321 atcaatgctc gggcgattcc aacccgccgc cgcatgcctc ctgaaagctc aataggcatt
     1381 ttatcgatcg catcttcgag attgacaaaa cgtagcattc gtctcacttc aggttcaacc
     1441 tcgtcttcgg gtacgccctg ctcgtgtagc cggtaagcga cgttctcgta aaccgataga
     1501 gaatcgaaga gagcgccttc ctggaagacc atcccgatct tcttgcgcac tttcatcatc
     1561 tgatcttctg agtagtcggt aatatcttcg ccatccacga ggacccgtcc ggtgtcgggt
     1621 ttcaatagac caagaacgag cttgatgatt gtcgacttac cgccgcctga gcctcccagg
     1681 atgatctttg tctcgccctt catcaccttg aaacttaaat gatccagaat tacgcgctcg
     1741 tcgaatgcga gcaggacatt gcgaaattcg atcgccggga tcgcccgctc acgatcgtcg
     1801 acttcgtgga atgtcgattc ggcggcgttt tcgctcaccg attgagaagt aatgtggatt
     1861 gactcgctag acattggatc gttgagcatt tatgacgtac ctagaataac ctgaagcgcc
     1921 tttgcgagaa agaagtcggc aataattaca actatcgagg ccatcacgac agctcgcgtc
     1981 gtcgagcgac ccacacctac tgtcccgcct tcggtgctca gtccctgccg gcaggcaatc
     2041 gctgcaatga tcaaggaaaa agctatcggc tttacaactc cgcctattaa atcgtcggtc
     2101 gtaataccgt ctcgaacggc tgaaacaaag acacttgacg agatggagta aagcgaaatc
     2161 gcggttaccc aacctccaaa aatgcccatt acgtccgcga gaaccgttag cagcggcaga
     2221 ctaacaagga gtgcaaacaa tcttggcgct acgagctttc ggatcggatc ggtgccgagc
     2281 gctcgcatcg catcgatctg ttgtgaaaca accatcgagc caagctcagc agaaatagct
     2341 gagccaacac gccccgtaac cataagggcg cataggaccg gaccgagttc gcgaaccagt
     2401 gaaatagcta ccagataacc agtttgcgat tgggctccat aagcctttag agttgggaat
     2461 gtctgaaccg tcagcacgcc acccgtaaaa aagcccgtaa gaacaatgat ggttagcgaa
     2521 ccgacgccga tggcgtccat ctgggcaatc gtttcaggga tgtatcgagg gcgcttaaat
     2581 ataccgcgga atgctcgggc ggccagcagt gttgtctcct gcaattccca aacggcgcgc
     2641 gtcgcaaagt tcatggaagg cgaaatgaat gccagtgttc aactgtggtc gagcttaagt
     2701 ggtgaaagtt ggtcaaactt agaagaaatt ggaattgctg ctcacttggt tcagcaatcc
     2761 cgaaacggtt taagttccag tacagtggaa tcgaagcggc ctatcatgcg caaattagac
     2821 gcagggcgtc aagcgtgaaa atttcggcga ggcctagact tttagggatt ttcagtcgtt
     2881 gcggttagct tcagggaagt cttgatcaaa aattcgcgac taatttactt actcaaccgc
     2941 tccgaataga ttgcggccga cctactaacg acgtggcgct acgtttcggg gtaggcccat
     3001 tgtaccggcc cgccgtcttg gattcggtaa tgatctcagg acgtccctgg tccgttggcg
     3061 cagcagacgc caggttgcaa tttgatctgg agtgagcact cttcgaatct ggacttcatt
     3121 ctgaattcgc attcttagtt gagcaccttg agccgcattg acttctgtta ttcgctgctc
     3181 aaccacactc tcatccaaat tgtcagaatc aagggcttgc tcaagggcga gatttgattc
     3241 gcgcaaccgc tggtttactg ccattcgttc atccttgctt tgttctcgta ttgcccgaat
     3301 cttttgtctc tgttcaggag tcagccgaag ttgttcgatc aagtcaggag cttgttggcc
     3361 cgcttgaggt tcctgcgaaa aagcgacagc gctcgcagag aggacgaagc caagaactag
     3421 ggtaagtagt aatgatttat ggttcagcaa catgaaacct caaactccta ctgatttatt
     3481 cgatcactga gtaattgaag accatcatcg tcaggtctgg tgacaagccg taaatcggaa
     3541 gccaattgtt gacgttcatc tcttgataac gggcgccggt tttttggtga tggagaagga
     3601 tttcggttga cgctcattcg acctgttgac tcttgagaag gtgtggagct ccgttcaatg
     3661 ccggcttgtt tagctgcgat ggtcgcctca gtgactgcct tcgccactgc gtctttgagc
     3721 tcggcttctg agtacacctt ggtgcgagct gcaataactg gtgggctagc cacaggtcgt
     3781 tccatcctcc tgaagacaat gaacccgaag acacacaaaa ctgctgtggc gagagccgtt
     3841 ccaaacttca tccatgcagg agaaagttcg aaaaaggcct ttagcgcggc acgcgccgat
     3901 tttcgttcaa cctttcgcga gatcgaagct gattggaagc caacaagcgc ttgatctctc
     3961 aaatcgcgaa ctgaggtgcg aactcccgag aatgcttcta actgttcatt gcagctcgca
     4021 cactgtcgaa ggtggctttg gaactgcctt atatcttcag ctgaagcttc gccataaaga
     4081 actgagatta gttcgtcttg acggttacat gcatgcgcaa ggaaatcttg attcatttca
     4141 tagtcctgac gtttttaagt cgcgtcattt gcgaatttct gaagtctcat ctggagctgc
     4201 ttcagggcag tgtaaagtcg gcttttgact gtgcttaagg gaatatcaag tgcctcggaa
     4261 atctcgcgaa atgtgagttc ttcaaactct ttcatgacta caacctgtcg cagctctaca
     4321 ggcagacttt ggatcgctcg acgtaccgca tctgtctcct gctggctctc aatgacatgg
     4381 gacggagagt aattaatggg tgcggcgaaa tcggcacctt gtttctcctg ttcatcgtcg
     4441 agagctgact cacttcgtac tttggctcgt cgctgtctgg taatgcattg atttactgca
     4501 atccggtgta accaggaaga gaccttagct tcaccccgaa accctcgcag attgcgaaac
     4561 gcggccagaa acgtttcctg tgttgcatcc cgtgcttcat cttcgcgacc gagcatgccg
     4621 taactgagcg cgaagattcg acgctcccag cggcgcacga tctccccaaa agcctctgga
     4681 tctccggcca gtgctcgttc cacaatcgtt tcgtcgttgg ttagagtgtc cattcgaccg
     4741 cgaactcagc tggcgatgcg actggtgagt ggccgttgtg tcataccgct caacgattta
//
LOCUS       DASSFP010000078         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1060338, whole genome shotgun sequence.
ACCESSION   DASSFP010000078 DASSFP010000000
VERSION     DASSFP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060338"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /gene="radA"
                     /locus_tag="VFH91_01595"
     CDS             complement(<1..1101)
                     /gene="radA"
                     /locus_tag="VFH91_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET6861700.1"
                     /translation="MKNSATVFICQSCGHQSQKWLGKCPECGEWNSLVEERAPLTKKG
                     SARNAFSVREVTAVPFTDIESQDDVRICTGLTEFDRVLGGGIVPGTLVLIGGDPGIGK
                     STLLLQVADHLSLAGTIVLYVSGEESERQVKLRGERLGVQAKHVYLLPETNLERIFLE
                     IDKLKPGAIIVDSIQTVFSSLIDSAPGSVSQVRDVAHHFLLLAKNRGIPVFLIGHITK
                     EGSIAGPKALEHIVDTVLYFEGERHHNHRIVRATKNRFGAANEVGVFEMTGTGLVPVT
                     NPSQIFLQERPQNVAGSIVTACMEGTRPLLVEIQALVSSSKYGSGRRMTQGVDQNRVS
                     LMIAMLEKRAGLQLIGDDVFINIAGGLEIDEPA"
     gene            complement(1229..1582)
                     /gene="arsC"
                     /locus_tag="VFH91_01600"
     CDS             complement(1229..1582)
                     /gene="arsC"
                     /locus_tag="VFH91_01600"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HET6861701.1"
                     /translation="MKDQITVYEKPTCTKCREMDKLLRERGVDFNKVNYYIQPLSKKK
                     LTELIRKMGIKPRELLRKSEPVYKELGLAKDEFSDTELISLMVEHPDLLQRPIVERGE
                     RAVLGRPTENVEELL"
     gene            complement(1606..2874)
                     /locus_tag="VFH91_01605"
     CDS             complement(1606..2874)
                     /locus_tag="VFH91_01605"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6861702.1"
                     /translation="MATKRAFEEELNPFAIAKQQFNRAADYLDLDLSMRHVLENAKRQ
                     LIVSIPVKMDGGDVQVFEGYRVQHNIARGPAKGGIRYHPNVTLDEVKALASWMTWKCA
                     TVGIPYGGGKGGVICDPKSLSRNELERLTRRYAFEIAPIIGPDRDIPAPDVYTDEQTM
                     AWIMDTISMVRGHTELGVVTGKPISLGGSQGRAEATARGCLYALREACRVKGLELKDS
                     RVAVHGFGNAGANIARLVAEDGARVIAACDSKAGIFSSTGIDIAAALKHKSETKSLYG
                     VPGTKEISPEEVISIDCDILLPSALENTITLKNVGQVKAKIIAELANGPTTPGADRVF
                     ENGDVFLVPDILANAGGVTVSYYEWVQDQNSFFWSEKLINETLEQTIRTAFNSVHEMS
                     KRYETDMRTGAYILAVGRVVEATSVRGIFP"
     gene            3234..>3667
                     /locus_tag="VFH91_01610"
     CDS             3234..>3667
                     /locus_tag="VFH91_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861703.1"
                     /translation="MFNNLIESSSHAQEFKRRGSFLLFTTITYALMLLITGVVSIYAY
                     DAHLGQQNLDFEVTLLTPETLSAEPERPTTAPAAGSNTPSHSNEPSDIRRQPTARVDT
                     PQLTPDTVSSAPNPNLPVRDGRWKIGDTDSNFQPDGPGSSSRG"
BASE COUNT          851 a         1005 c          878 g          933 t
ORIGIN      
        1 tgctggttca tcgatctcca gaccgcctgc aatgttgata aatacatcgt caccgattaa
       61 ctgcaggcct gcgcgcttct ccaacatagc aatcatcaga gacacgcgat tttgatcgac
      121 gccctgcgtc attcgtctgc cactaccgta ctttgagctt gaaacaagcg cctggatctc
      181 caccagcaac ggacgggttc cttccataca cgccgtaaca attgaaccag caacattctg
      241 tgggcgttct tgcagaaaaa tctgcgaggg atttgtcact ggtacaaggc ctgtcccagt
      301 catttcaaag acaccgactt cattcgctgc cccaaatcga ttttttgtgg cgcgcacgat
      361 gcgatgattg tgatgacgct caccctcaaa atacagaacc gtatcgacaa tatgttcgag
      421 cgcctttggg ccagcgatcg acccttcctt agtgatgtgt ccaattagaa atacgggaat
      481 gcctcgattc ttcgcgagca acaggaaatg gtgtgcaacg tcgcgaacct gcgagaccga
      541 gcccggggca gaatcgatca aactcgaaaa gactgtctga atcgaatcaa caatgatcgc
      601 gcctggcttg agtttgtcga tctcaagaaa aattctttca agattagtct caggcaacag
      661 gtaaacatgt ttggcctgaa caccgagtcg ctcaccgcgc agtttgacct ggcgttccga
      721 ctcttcaccc gaaacatata aaacgatggt ccccgcgagg ctcaggtggt cagcaacctg
      781 aaggagcaga gtgcttttac caatacctgg atcgcccccg attagcacga gggttcctgg
      841 gacgatgccg ccacctaaga cccgatcaaa ctcggtgagg ccagtacaaa tgcgaacgtc
      901 atcctgtgat tcaatatcgg taaaagggac tgccgtgact tcgcgcacgc tgaaggcatt
      961 tcgagcgctt ccctttttgg tcagaggcgc gcgctcctca accagtgagt tccattctcc
     1021 gcactcggga cacttaccga gccacttttg agattggtgg ccacagcttt gacagataaa
     1081 gacggttgca gaatttttca tgtggatgcg aggctttggc ctttggcctt tgatctttgg
     1141 cctttgagat ctgaaatttg aaattttacg gtttagagct gacgaccaag cttcaaaggc
     1201 caaagatcaa aggccaaagg ccgatgacct acaacaactc ctctacattt tcggtgggtc
     1261 gacccagcac tgctcgttcg ccacgctcta cgattggtcg ttgcaacaga tcaggatgtt
     1321 cgaccatcaa ggagatcaac tccgtatcgc tgaattcatc cttggccagc ccgagctcct
     1381 tataaaccgg ctcactcttg cgcagtaact cgcgcggctt gatccccatt ttacgaatca
     1441 actctgttaa cttctttttc gacagcggtt gaatgtagta gttgaccttg ttgaagtcca
     1501 caccacgctc gcgcagcaac ttatccatct cccgacactt cgtgcaagtg ggtttttcgt
     1561 aaacagtaat ttgatctttc atagttgaac ttgccggctc cttgatcagg ggaaaattcc
     1621 tcttacgctg gttgcttcga ctacccggcc gactgccagg atgtacgcac cggttcgcat
     1681 gtccgtttca taacgctttg acatttcgtg caccgaatta aatgcagtcc tgattgtctg
     1741 ctcaagcgtc tcgttgatca gcttttctga ccagaagaac gaattctgat cctgcaccca
     1801 ttcgtagtaa ctcaccgtaa ctccaccagc attggcgagg atatcgggaa cgagaaacac
     1861 gtcaccattt tcaaatactc gatcagcgcc aggtgtggtt ggcccattag cgagttctgc
     1921 aattatcttc gctttgacct ggcccacgtt cttcaacgtt atcgtgtttt caagggcaga
     1981 gggtaaaagg atgtcgcagt cgatgctgat tacctcttcg ggtgaaatct ctttagtacc
     2041 cggcacgcca tacagagact tggtctcgga cttatgtttc aaagccgcgg caatatctat
     2101 tccagtgctc gagaaaatcc cggctttcga gtcgcacgca gcaataactc gcgctccatc
     2161 ttcggccacg agcctggcga tgtttgctcc ggcgttgccg aatccgtgca ctgctacccg
     2221 cgaatccttc agttctaatc ccttaactcg acacgcttca cgcaacgcgt agagacagcc
     2281 acgagcagtt gcttcggccc ttccttgtga tcctccaagc gagattggct ttccagtgac
     2341 gactccaagc tctgtgtgac cgcggaccat cgagatcgta tccatgatcc atgccatcgt
     2401 ttgttcatcg gtgtagacat cgggggctgg tatatctctg tctggtccaa tgattggcgc
     2461 aatttcaaat gcgtaacgac gggtcaatct ttccaattca ttccttgaca ggctcttggg
     2521 atcgcaaatc actcctcctt ttccgccccc gtagggaatg ccaaccgtcg cgcacttcca
     2581 ggtcatccat gaggccaaag ccttgacttc atcgagagtc acgtttgggt gataacggat
     2641 gccacctttg gctgggccac gagcgatgtt gtgctgtact cgatagcctt caaacacttg
     2701 aacgtcgcca ccatccattt tcacgggaat tgaaacgatg agctgtcgtt tggcgttttc
     2761 gaggacatgc cgcatggaga ggtccagatc gaggtagtcg gccgctcgat tgaactgctg
     2821 tttagcgatc gcgaacggat tcagttcttc ctcaaaggct cgtttagtcg ccatgttcta
     2881 ctcctcggat tggattgagg aaagcacatc aatgctattg gtacaaggtg ggaatggcaa
     2941 gcgcctcatc acctaaggct ttgtgctttg cgctttgccc tttgtgtttt gcgttttgcg
     3001 ctttgcgctt tgtgctttgc gcttttgttc ccctgggcca gaatcatgca cttgggaagg
     3061 atgcacaaaa gtactaagta caaagcacaa agtcgggaac ttgtaccctc agtgagtgtc
     3121 taacctaatt gagtaagctc ccgcgacccg acggcaccgg catttgcgga actcgattca
     3181 gacttcgcat tgttgcatgc caaggtggtg tcgagccgca gggaggactg aggatgttta
     3241 ataacctgat cgagtcgagc tcgcatgctc aggagtttaa gcggcgtggc tcattcctgt
     3301 tgttcacgac tatcacttat gcgctgatgc ttttgatcac tggtgtagtc agcatctacg
     3361 cctacgatgc gcacctgggc caacaaaatc ttgatttcga agtaaccctg cttacgcctg
     3421 aaactctatc tgctgaacct gagcggccaa ccacagctcc tgcggcagga tcaaacacac
     3481 catcacacag caatgaacca agtgacattc ggcgtcagcc gacggcccga gttgatacgc
     3541 ctcaacttac gccagacacg gtctcatcgg cgcccaatcc aaatctacca gttcgagacg
     3601 gcaggtggaa aattggggac accgattcaa attttcaacc cgacggacct ggcagctcgt
     3661 ctcgcgg
//
LOCUS       DASSFP010000079         4150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_608919, whole genome shotgun sequence.
ACCESSION   DASSFP010000079 DASSFP010000000
VERSION     DASSFP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608919"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFH91_01615"
     CDS             <1..524
                     /locus_tag="VFH91_01615"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HET6861704.1"
                     /translation="IEALTVIGFLLVVLSSCSKPAVDQPRSSSAPATSPAKISSVAVV
                     KVNADEVDVSVGGSAEAKVRLSIDSGYHVNANPPTFPYLKPTELAINSTPPILVGSTI
                     YPGATTRKFQFADKPLAVYEGETVIKVQLKVDKGASQGKQNLSGKLRVQACDEEVCYP
                     PGERDIVIPVNIK"
     gene            521..721
                     /locus_tag="VFH91_01620"
     CDS             521..721
                     /locus_tag="VFH91_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861705.1"
                     /translation="MKIFWMAIAGVCAVSAGVFLLKRDFDKAFIVAAAGAVAWFLNYR
                     VQMREVVDRADQEENDDLSDQK"
     gene            696..1244
                     /locus_tag="VFH91_01625"
     CDS             696..1244
                     /locus_tag="VFH91_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861706.1"
                     /translation="MTTYQIRNKFVLAFVLIILGFAQTTIASDQTEVRGVVQRVFEQL
                     RNHDYNSLYDLLPNTSRSRLSRERFVSALERAQGFYQLDRLEIGSLKVQGNLAVVDTV
                     LYGRVVAPVQAEGKIVAQQYLVREDGHWRVATGDQSTVKKFLASTPGFSKRFKIRQPQ
                     VFVKQNGQWIEFKGPRGRRNQA"
     gene            complement(1245..1496)
                     /locus_tag="VFH91_01630"
     CDS             complement(1245..1496)
                     /locus_tag="VFH91_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6861707.1"
                     /translation="MEEREEFLTAKQLAQFLQVSESTVRRLAAKRRIPSVRLTPRIIR
                     FHLDAVREALDVPRHRIRHGEYTEPVDDAQLSFDDFFLS"
     gene            complement(1500..2126)
                     /locus_tag="VFH91_01635"
     CDS             complement(1500..2126)
                     /locus_tag="VFH91_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET6861708.1"
                     /translation="MRFKKSIVTVLFGLLACSFAFGQDKTTGSIKGKVRVESGTPNGV
                     TVTVRQGDHELNHVSTDKSGKFVIERLAPGSYGLTFRKPGLSIGTIENVVVKAGNVRS
                     LGDRLVLTIDEGSIAFIRGSVFSGSGRSVPNAKVELAKVNADGTTKKIDGRITTETGS
                     FVFRLAPEAAKYRVTLKTDGEPISRDVEVEGAAVYRVALSLPEKPAKP"
     gene            2324..3358
                     /locus_tag="VFH91_01640"
     CDS             2324..3358
                     /locus_tag="VFH91_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6861709.1"
                     /translation="MAQTLAAKFLLLILLVITITAVCESQTTKTPETSQRPRKVFPAE
                     QQAEDVIRVDTDLVSVDVTAIDSEGRPVRNLRPQDFKVYSDGVEQQVSFFQIEKRQGE
                     PRPLAVVFALDVSGSMTSEEMNRLRIALKSFSDQLSSHSVLYAVMTFGMKVKTVQKLT
                     SQPNKLDDTLERLTRDAPNGLSTHTYDAVDDAIRLLVRNAPRTREHRLMKRTVLVVTD
                     GFPVGDTVAPQTVIERANSADVSVYVVTLPSYSRMMASSAQTPLPTPLDVSGLAELTG
                     GRNVYANEQDYVPLFKSLAEEVGSAYVLAFYPPEEKRRDGKIHTIRVEGPTGLTLRQS
                     RLEYRAAGKE"
     gene            complement(3436..3621)
                     /locus_tag="VFH91_01645"
     CDS             complement(3436..3621)
                     /locus_tag="VFH91_01645"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861710.1"
                     /translation="MPTGTCPECDADVHVDMDTDKGEIVSCEECGTELEIVGLDPVEL
                     DIVEEEDYDEDEDEDEY"
BASE COUNT         1065 a         1064 c         1020 g         1001 t
ORIGIN      
        1 aaattgaagc cttaaccgta attggatttc tcttggtggt tttatctagt tgttcgaaac
       61 ctgcggtgga tcagcctcgc agctcatcgg cgccggcaac atctcccgca aagatttcct
      121 cggtggcagt tgttaaggtc aacgctgatg aagttgacgt gagtgtcggt ggctcggctg
      181 aagctaaggt acgactaagc atcgatagcg gctaccacgt aaacgctaac ccacccacct
      241 ttccatatct gaaaccaaca gagttggcga ttaattcaac gccgccgatc ttggtcggct
      301 caacaatcta cccaggcgct accacaagaa agtttcaatt cgcagataag ccgctggctg
      361 tttacgaagg agagacagtc atcaaagtcc agctcaaggt ggacaaagga gcttcacaag
      421 gaaagcagaa cctttccggc aaattacgcg tccaagcgtg tgatgaagaa gtatgctacc
      481 ccccaggtga acgtgacatt gtgattccgg tgaatatcaa gtgaagatct tttggatggc
      541 aattgctggt gtttgcgcgg tttccgccgg agttttccta ctcaaacgcg attttgataa
      601 ggcgtttatc gttgccgcgg ccggggccgt cgcgtggttt ctgaattacc gggtacaaat
      661 gagagaggta gttgaccgag cagatcagga agagaatgac gacttatcag atcagaaata
      721 aatttgtttt ggcgtttgtg ctaatcatcc ttggattcgc acaaacaaca atcgcatcgg
      781 accagactga agtaagaggt gtcgtgcagc gcgtcttcga gcagctgcgc aatcacgact
      841 acaacagtct ttacgacttg ttacccaaca cttccagaag cagattgtcg cgagagcgct
      901 ttgtgagtgc gctcgaacga gcccagggct tctaccagct cgatcggctc gagatcggat
      961 cattgaaggt ccagggaaat ctggctgtag tcgatacagt tctttacggc agagtggtgg
     1021 cgcccgtcca ggcagaaggc aaaatagtcg ctcaacaata cctcgtccgg gaagatgggc
     1081 actggcgtgt cgctaccggc gatcagtcga cagtgaagaa gtttctcgca agcacgccag
     1141 gattcagtaa acgatttaag atccgccagc cgcaagtctt cgtcaaacag aatggacagt
     1201 ggatcgagtt taaaggacct cgaggcaggc gtaaccaggc gtagtcagct caggaaaaag
     1261 tcgtcgaaag acagctgcgc atcgtcgact ggttctgtgt actcgccatg acgaatgcgg
     1321 tgacgcggca cgtctaacgc ttccctcaca gcatcgaggt gaaaccgaat gattcttggt
     1381 gtcaatctta ccgaagggat acgacgtttc gcagcaagcc gcctgacagt cgattcgctg
     1441 acttgcagaa actgggccaa ttgtttagct gtcagaaact cttcccgctc ttccatgcat
     1501 tagggcttcg caggtttttc gggcaatgaa agcgcgaccc gataaaccgc cgcaccttca
     1561 acttccacat ccctgctgat cggctctcca tcagttttta aagtaactcg atactttgcg
     1621 gcttccggag cgagtcgaaa cacaaatgaa cctgtttcag tagtaatccg gccatcgatc
     1681 ttcttagttg taccgtcggc atttaccttt gcgagctcga ccttcgcatt cgggacgctc
     1741 ctgcctgagc cactgaaaac actacctcga atgaacgcaa ttgagccctc atcgatcgta
     1801 agtactaacc ggtcacccag tgagcgcacg tttccggcct ttaccaccac gttctcgata
     1861 gtaccgatac tcaatcccgg tttccggaac gtaaggccat aactacccgg tgcgagccgc
     1921 tcgattacga atttaccgct tttatcggtg gacacgtgat ttagttcgtg gtctccctgg
     1981 cgaacagtga cggtaacgcc atttggagtg ccagattcga cgcgaacctt gccttttatg
     2041 gaacccgtag tcttgtcctg accaaatgca aagctgcatg ccagcagtcc aaacaaaact
     2101 gtcacgatcg attttttgaa tctcattctc tcgtctccac gtagtatagg ctgctgagaa
     2161 tattcgcctt gataatacat cacaggttga aaaatcgcct ctgtcgaggg gcctgggtga
     2221 gccggtaaag gagtccgttc cagccagact ttgatgcgcg caagtggtaa gaaaattcgc
     2281 ctgctcgtat aattcgctct acgtattatt aaggaagtgt tagatggcac aaacactggc
     2341 ggccaaattc cttctcctca ttcttctcgt aattactatt accgccgttt gcgaatcgca
     2401 aacaacaaaa actcctgaaa catctcaacg acctcgaaag gtttttccag cggagcaaca
     2461 ggcagaagat gtgatccgtg tggacactga tctggtttca gttgatgtta cggcgatcga
     2521 ttctgaaggt cggcccgtcc gcaatctgcg tccgcaggac tttaaggtct actctgatgg
     2581 tgtcgagcaa caggtctcct tctttcaaat cgaaaagcgg caaggagagc caagaccgct
     2641 ggccgtagtg ttcgcactgg acgtctcagg tagcatgacc tccgaagaaa tgaaccgctt
     2701 gcgaatcgca ctgaaatcgt tttcggacca actgtcgagt cactccgtcc tctacgctgt
     2761 catgacattt gggatgaaag tcaaaaccgt tcagaaactg acgagccaac caaacaagct
     2821 tgacgatact cttgagcgtt tgacgagaga tgctccgaac ggtctatcaa cccacactta
     2881 cgatgcagtc gatgatgcga ttcgattgct tgtccgaaat gcgccgagaa cacgtgagca
     2941 ccggttaatg aaacgcactg tccttgtggt gactgacgga tttccagtcg gagatacagt
     3001 tgcgccacaa acggtaatcg aacgagctaa ctcagcggac gtcagcgttt acgtcgttac
     3061 tttaccttcg tattctcgaa tgatggcatc gtcggctcaa acgcccttac caacaccact
     3121 cgacgtcagc ggactggcag agttaactgg cggtagaaat gtttatgcca acgaacaaga
     3181 ttacgtacct ttattcaaat cgttggcaga ggaagtgggc tcagcgtatg tgctcgcgtt
     3241 ctatccaccc gaggagaaaa gaagggatgg caagatacac acgattcgcg tcgaaggacc
     3301 aactggcctt acgctacgtc aaagtcggct ggagtatcga gcggcaggca aagagtaaag
     3361 ccacagaggc aatggaagca ggaacatcag cgaggggata attcaaccag ccaattgttc
     3421 ctgctccgat agggatcagt attcgtcttc gtcttcgtcc tcgtcgtaat cttcttcctc
     3481 gacgatgtcg agttcaacag gatcgagccc aactatttct aattcggttc cacactcttc
     3541 gcaagaaaca atttcgccct tgtcggtgtc catatcaacg tggacatcag catcacattc
     3601 agggcaggtt ccggtcggca cggtttattc ctccaacgca tttttatcat ggcagtggta
     3661 cacccggtac tttagggaga gtccgctacc ttgtctttgt aaactcggtc aatctacccg
     3721 tcgacgcggg aatttgcaac actctttttc aagagatttt gccagggacg atctgggcca
     3781 actctgaaca aactattaac tgaaaatcga ctgactttaa gaaacaagaa gaccaaacct
     3841 ggagggtccg gcctacgtat tgcacccagg agaatctgaa attcagattt ttctcagtca
     3901 acagataaat agctcatgca cactgaaatt ccgccattcc cgtagctcca cgctttctca
     3961 aagttgcagg agctcgcgtg aaggtgcagc acgactcagt caggtggagt aaacagttct
     4021 gttacgatga gttgcagcat acggagataa atatgagatc gacagttcgt tgcctcacgc
     4081 ttctgctcct gatccttttc gctctaccgg caatcagtca agagaaacgt ccgcaatcca
     4141 gcagaggtcg
//
LOCUS       DASSFP010000080         4532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1805288, whole genome shotgun sequence.
ACCESSION   DASSFP010000080 DASSFP010000000
VERSION     DASSFP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805288"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFH91_01650"
     CDS             complement(<1..564)
                     /locus_tag="VFH91_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861711.1"
                     /translation="MFASRFRVLALLVLCLITTGVINFPSAARGEDNLFTGTLDGELV
                     PNTENMDQIIFRPLKDMSKVKFVSPPPTDAKLTAGRLYDPLRDKSAILTLLVLAEDEQ
                     PFIYADVDLDNSISDSERFPMQPENGRNPYILQTIIRLPFKNALFQNFPIIIQYFKDV
                     RWDELKEGETLLMQSKEAFALGKVDIGG"
     gene            complement(865..1779)
                     /gene="argB"
                     /locus_tag="VFH91_01655"
     CDS             complement(865..1779)
                     /gene="argB"
                     /locus_tag="VFH91_01655"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET6861712.1"
                     /translation="MTEPRPEVDNQLRLDLLREALPYIQRFKGQTFVVKLSGKVTENS
                     ENLASLAEELALLHQVGIRICVIHGGGKQLSDLASKLGVEQTIIEGRRVTDDATLEMA
                     KMIFAGKINTDILAALRQRSIEAVGLSGVDGNIVHAIRRPPKDILNRETGKSARVDFG
                     HVGDVVQINSRLLTVLLDHGYLPVISSLGADDEGTVFNINADTIAAEIAVQLQAEKII
                     LLSDVDGIYLRAGESDSKLSRLTTTEADELIASGAATGGMIPKLQSVTTLLRRGVHSA
                     HIISGTKRNALLSEIFTDKGTGTMIISD"
     gene            complement(1792..2799)
                     /locus_tag="VFH91_01660"
     CDS             complement(1792..2799)
                     /locus_tag="VFH91_01660"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HET6861713.1"
                     /translation="MQDFLTTANLDRGQLDHLIESAIRFKHGQDQSKPLTGKSVALVF
                     FNPSLRTRASMQVGIYELGGNAVTLEPGGSSWTLEHRHGAVMDGDKTEHISEFVRVLE
                     RYCVAIAVRTFADLKTWQEERADPVLHAFARYASVPVINLESAMHHPCQSMADMMTIQ
                     EKLGTGRKRVVLTWAWHPKPLPMAVPNSFALASAQMGHDLVLARPPEYDLDEELMESL
                     TLQASNAGGSVKVTDDIDAAFDQAEVIYAKSWGSKRFYGNSEQDLIKRAQYRRKWIVD
                     DQKMNRTDDAIFMHCLPVRRNVIVTDSVIDSPASVVIDEAENRLHMQKAVLAELFKEN
                     Y"
     gene            complement(2822..4027)
                     /locus_tag="VFH91_01665"
     CDS             complement(2822..4027)
                     /locus_tag="VFH91_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6861714.1"
                     /translation="MTVETITSLSEVSASEEQHQLATYKKMPVVAKRGEGVWLYTSDG
                     DKYLDLYGGHAVAGTGHCHPHVVQAIQKQSEQLLFYSNLVYSETRALAAEKLISVAPR
                     PLAKVFFCNSGTEANENAMRIARMKTSREKIITFSGGFHGRTADAISATFLGKYRELG
                     RPNVPGHLEAQFGDIESVRLLADDTVAAVMLEPIQSMAGVKTASPEFFVELRALCDEL
                     GILLIFDEVQTGVGRTGNWFFAGSEFAGNVVPDIVTLAKALGSGVPVGACLVSENAAS
                     AIKENDLGTTFGGGMLAMAAVSATLEAIEEDGMLGNVKRVEAYLREQLKTVESVVNVH
                     GNGFLLGIEFAGKAADVHKALLERKIITGTSSNPQTLRLLPPLTLSTEDIDMLINALR
                     EIELSQLNS"
     gene            complement(4024..4383)
                     /locus_tag="VFH91_01670"
     CDS             complement(4024..4383)
                     /locus_tag="VFH91_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861715.1"
                     /translation="MEFTPAPIKSLIQIEDVEKIDVRVGTILKVSDVENASKLMRLTV
                     SFGDHERTILAAIKNERENPKEIEGAQALFIVNLAPRKMRGETSEGMLFDIGYADGLT
                     PVLAVPEKSIKDGARAG"
BASE COUNT         1047 a         1195 c         1099 g         1191 t
ORIGIN      
        1 gccgccaata tcaaccttgc ccaaagcgaa agcctccttt gattgcatca acagggtttc
       61 gccttcctta agttcgtccc accgaacgtc cttgaaatac tgaatgatta tcggaaagtt
      121 ttgaaagagc gcattcttga atggcagtct aattattgtt tgcagaatat aaggattacg
      181 accgttctcc ggctgcattg gaaatcgctc gctgtcagaa atagagttat cgagatcgac
      241 atcggcatag atgaaaggct gctcgtcttc tgcaagcacg agcaaagtaa gaatcgccga
      301 tttatctcgt agcggatcgt aaagccggcc cgcggtaagt tttgcatctg tcggtggcgg
      361 cgagacaaat ttaaccttcg acatgtcttt cagtggtctg aaaataatct ggtccatgtt
      421 ttcagtattt ggaacgagtt caccatccag tgtgccggtg aacaggttat cttcaccgcg
      481 agctgctgag ggaaagttga tgacgccagt tgtgatcaaa cacagaacca gtagggcgag
      541 aactcggaaa cgcgaggcaa acatattcga agtccttcga gtgggattgt gagagcgatt
      601 cggattctat gtttggccga aatcgcgtgt caacaatttc gcctcgaggc tgatgagcca
      661 atgtagttct ctcattcacc tcagattcgc ctcgcatcat ttctctaatt ggcctctcat
      721 tcaccccagg gcttcagcct ggggatccgt ctagccccac ggcatgggga aaccgtttta
      781 acggtttacc tgcgcctgga gtccagagtt ttgtgaagta gagtgggaat tagtaattca
      841 taaatctcaa atcaaaattg agacttaatc ggaaatgatc attgtcccgg tgcctttatc
      901 agtgaagatt tcagacagga gcgcattccg cttggtgcca cttatgatgt gggcgctgtg
      961 aactccgcgt ctcaaaagcg tggtgacgct ctgaagcttt ggaatcatgc cgccagttgc
     1021 tgcgccactc gcgatcagct catccgcttc agtagtcgtc aaacgcgata gtttcgaatc
     1081 tgactcaccg gcccgcaaat aaataccatc cacatcgctt aaaaggatga tcttttctgc
     1141 ctggagttgt acggcgattt ctgcagcgat tgtatcggcg tttatattga agacagttcc
     1201 ttcgtcgtca gcgcctaacg atgagatcac cggcaaatag ccgtgatcaa gcagcacagt
     1261 taataagcgt gagttgatct gaaccacgtc gccgacatgt ccgaaatcga cgcgcgcact
     1321 ctttccggtc tcgcgattga ggatatcttt tggcgggcgc cgtatagcgt gaacaatgtt
     1381 gccatccacg ccggacaatc ccaccgcttc gatgctgcgt tgacgaagcg ctgccaggat
     1441 gtcggtattg atctttcccg cgaagatcat tttcgccatc tcgagcgttg cgtcatccgt
     1501 cacgcgacgt ccttcgatga tcgtctgttc cacaccgagc ttcgaagcaa ggtcactaag
     1561 ttgtttcccg cctccgtgaa tcacacagat gcgaatccca acctggtgca acagggccag
     1621 ctcttcggcg agcgaggcga ggttttccga attctcggtt accttgcctg aaagtttcac
     1681 tacgaaagtt tgccctttga atcgctggat atacggaagg gcctcacgca acaaatcgag
     1741 gcgtaactgg ttatcgactt caggacgcgg ttcagtcata cggacttgct ttcagtaatt
     1801 ctccttgaag agttcggcaa gcactgcctt ttgcatgtgc agtcgatttt ctgcttcatc
     1861 gatcacgaca gatgccggag aatcgatcac gctgtcagtt acaatcacat ttcgtctgac
     1921 cggcaaacag tgcataaaga tcgcgtcatc cgtgcggttc atcttttgat cgtcaacaat
     1981 ccacttccgg cgatattgag ctcgtttaat gagatcctgc tcagagtttc catagaagcg
     2041 ttttgagccc cagcttttgg cgtagatcac ttcagcctga tcgaaggcgg cgtcgatatc
     2101 gtccgtgact ttcacacttc ctccggcatt tgacgcctgg agcgttagcg attccatcaa
     2161 ctcttcgtcc agatcgtatt caggtggacg agccaatacc agatcatggc ccatctgagc
     2221 cgatgcaaga gcaaagctgt tcggcactgc catcggcaac ggctttggat gccaggccca
     2281 ggtcagaacc actcttttcc ggcccgtgcc gagcttctcc tggatagtca tcatgtcggc
     2341 catggattgg cagggatgat gcatcgcaga ttcgaggttg ataactggaa cacttgcata
     2401 ccttgcgaag gcgtgcagta ccggatcagc ccgttcttcc tgccaggtct ttaaatcggc
     2461 gaaggttcta acagcgattg ccacgcagta acgttcaagg acccgaacaa attccgaaat
     2521 gtgttcggtc ttatcgccat ccatcacagc accgtgacga tgctccaagg tccaactcga
     2581 gccgccaggc tctagcgtga ccgcattacc gccaagttca taaatgccga cttgcatcga
     2641 tgcccgcgtc ctcagacttg ggttgaaaaa aaccagtgct acagattttc ccgttaacgg
     2701 tttggattga tcctggccat gcttgaaacg tattgccgac tcgatcagat ggtccaattg
     2761 gccacggtct aaatttgcgg ttgttaagaa atcctgcatc ttgataattt cggtctatta
     2821 actacgagtt tagctgcgac aattcaattt cacgaagtgc attgataagc atgtcaatgt
     2881 cttctgtcga cagtgtcaac ggtggcaata atcgcaaggt ctgtgggttg ctggaagtcc
     2941 cggtgattat ctttcgctca aggagtgctt tgtggacgtc agctgctttg cccgcgaatt
     3001 cgatgccgag tagaaaaccg tttccgtgta cattcactac agactccaca gtcttgagtt
     3061 gttctcgaag gtacgcttcc actctcttaa cgttgccgag catgccgtct tcttctatcg
     3121 cttccaatgt tgcagagaca gcagccatcg ccagcattcc accaccaaag gtagttccaa
     3181 gatcattctc ttttatggcg ctggccgcgt tctcgctaac cagacacgcc cctacaggca
     3241 caccgcttcc cagtgctttg gcaagtgtga cgatgtcagg tacgacgttg ccggcgaatt
     3301 cacttcctgc aaaaaaccaa ttgcctgtac gaccgacacc agtttgcact tcatcgaaga
     3361 tcaagagtat gcctagctca tcgcagagcg cgcgtagctc aacaaagaat tccggactcg
     3421 cagtcttcac gccagccatc gactggatcg gttcgagcat tacagcggca acagtgtcat
     3481 ccgccagtaa tcgcactgat tcgatgtcgc cgaactgagc ctcgagatga ccgggaacat
     3541 ttggtctgcc gagctcacga tattttccca ggaaggtcgc gctaattgcg tcggccgtgc
     3601 gtccatgaaa gccgccggaa aaagtaatga tcttttcgcg actagttttc attcgtgcaa
     3661 tacgcatggc attttcgttt gcttctgtgc cggaattgca aaagaaaacc ttagcgagtg
     3721 gtctaggagc aactgaaatc aacttctctg cggccagcgc acgtgtctcg gaatagacga
     3781 gatttgaata gaaaagcagc tgttcggatt gtttctgaat agcttgaacg acgtgtgggt
     3841 gacagtgacc ggtgccagct acggcgtgcc ctccgtagag atcgagatat ttatcgccgt
     3901 cactcgtgta gagccagact ccttcgccgc gctttgcgac gacaggcatc ttcttatacg
     3961 tagcaagctg atgttgctct tcgcttgctg acacttcgga aagagaagtt attgtttcca
     4021 cagtcatcct gctcgtgctc catccttgat cgacttttcc ggcactgcca aaaccggcgt
     4081 taatccatcc gcatatccga tatcgaacaa cattccctct gacgtttcgc cgcgcatttt
     4141 tcgcggcgca agatttacga taaagagtgc ttgcgctcct tcgatttcct tagggttctc
     4201 tcgctcgttc ttgatggcgg cgagaatggt gcgctcatga tctccaaacg agacagttaa
     4261 cctcatcagc ttactggcat tctcgacatc acttactttc aagatcgtgc ctacgcgaac
     4321 atctatcttt tcgacatcct ctatctggat cagtgactta attggtgctg gtgtaaattc
     4381 cattttgttc gtgattattc gtcgattgcc ggagggtggc cacggagggc cacccctaca
     4441 atcatgggtt cgttccgacg agccggaggc cggtcgtttc gtcgagacca aacatcaagt
     4501 tcatgttctg cactgcctgg cccgccgctc ct
//
LOCUS       DASSFP010000081         4384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1060965, whole genome shotgun sequence.
ACCESSION   DASSFP010000081 DASSFP010000000
VERSION     DASSFP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060965"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1178)
                     /locus_tag="VFH91_01675"
     CDS             complement(15..1178)
                     /locus_tag="VFH91_01675"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6861716.1"
                     /translation="MWDVIIIGGGAAGLFCAIESGKRKRKTLVLEHNSRVGRKIEISG
                     GGRCNFTNIHTTPDAFLSQNPHFAKSALARYSAEDFISLVKKHRIRFHEKKLGQLFCD
                     SSSQGIIDMLLTECSATGVVIKSGCHVSSVSHSGSFKLETDSGVFQCSSLVIATGGLS
                     IRPLGATDFGYRMANQFGLRIEQTKPGLVPLVLDDSLRTRLAKLSGISVDAVVTCTGT
                     NFRENLLVTHRGLSGPAILQISSYWKPGNTVSINLLPDDDAFSLLKSNHNSETELANV
                     LSRYLPRRFAHAWCELNAPSQPMKRYTEPDLERIAANLHALNLVPTGTEGYKKAEVTA
                     GGVSTAELSSQTMEAKRVHGLYFIGEVVDVTGHLGGYNFQWAWSSAFAAGQHA"
     gene            complement(1202..1777)
                     /locus_tag="VFH91_01680"
     CDS             complement(1202..1777)
                     /locus_tag="VFH91_01680"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET6861717.1"
                     /translation="MTTSRMEAFSDGVIAILITIMVLELRPPHDATVQGLRPLIPILL
                     SYLVSFVFLGIYWTNHHHLLQAVEHVNGSVLWANLHLLFWLSLIPFVTAWMGENDFAK
                     WPVALYGIVLLLAAIAYFILVKTLLALHGRNSVLADAIGNDFKGKISMVILVIAVPLA
                     FVRPWIACLFYGLIAVMWLVPDRRIERTMNP"
     gene            complement(1792..3531)
                     /locus_tag="VFH91_01685"
     CDS             complement(1792..3531)
                     /locus_tag="VFH91_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6861718.1"
                     /translation="MKISKAPQLALTVGTILFYASLTLFAQQANFQMPSGPLSFGVFT
                     ARFDAGGTFTLEGDRWPKLTGNWKTDGSEIQLTMSGGPGGCDQPGRYKLAKNNNRLTF
                     DLVSDECQPRQMILDRSTWLPSGEKVDKPVRQINVTTVKTLRSSTKPADSSGSWPSFR
                     GPHASGVVEGQNLPDEWSPKTGNNILWRTLIPGLAHSSPVVWGNTVFVTSAISSDPKA
                     NFRPGLYGDGDASKDHTVHRWVIYAIDKSTGKISWERVAYQGEPIEKRHIKATYANST
                     PATDGRIVVAWFGSQGVYAYDVSGTFLWKVDLGRIDLGAYDIPTFEWGSASSPIIWND
                     LVILQCDTQTDSFIIALDAKSGKTIWKTKRDEIPSWGTPTIASTSKGVELIANASNFI
                     RGYDPKTGKELWRLGGSSKITAPTPIYADDVLVVASGRGPERPIFVIKAGAQGDLTLP
                     EGKTSSDAVLWSKTGRGSYMPTPLIYKGVLYVLANNGLFDAYDLKTGEELYRQRLPLV
                     GSGFSASPVAADGKIYLSNEDGEMLVVSAGPKFAHLATNSMGELLMATPAISEGVMYV
                     RSSSSLFAIGRKK"
     gene            complement(3552..4067)
                     /locus_tag="VFH91_01690"
     CDS             complement(3552..4067)
                     /locus_tag="VFH91_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HET6861719.1"
                     /translation="MNNLLLVLLMTLIAVFQAPQESRPIRNFLRVNDQFCTGGQPQLD
                     QLEKLKSEGVKAIINLRQPSEHRAAEEEAKAKELGLRYFNIPFVFGDPKDEQATEFLR
                     ITDDPENRPAFIHCTAGIRVGAFWMIRRVLRDGWSIKDAEAEAEKVGLKDSPHLNEFA
                     RKYIEKYGKKQ"
BASE COUNT         1101 a         1107 c         1100 g         1076 t
ORIGIN      
        1 tttgagtgca gtttctaagc gtgttggccg gcggcgaaag cggaggacca ggcccactga
       61 aaattgtagc cacccagatg tccggttaca tcgacaactt cgccgatgaa atatagacca
      121 tgaacccgtt ttgcttccat cgtttgagac gacaattcgg cggtgctaac gcctcccgct
      181 gttacttctg cctttttgta tccttcagtg ccagtcggaa ccaggttcaa agcgtgcaaa
      241 ttagccgcta ttcgttctaa gtcaggttca gtgtaacgtt tcattggttg agagggagcg
      301 ttaagctcgc accacgcatg cgcaaatcgc ctgggaaggt aacgcgagag cacatttgcg
      361 agttcagtct cagaattgtg attgctcttg agtaagctaa acgcgtcgtc atccggcagt
      421 aaattgattg agacagtgtt gccaggcttc caataggacg aaatctgcag gatagccggt
      481 ccacttaacc cacgatgtgt caccagcagg ttctcgcgga aatttgtacc tgtgcaagtc
      541 acaaccgcgt cgactgaaat cccactcagt ttagccaggc gggtgcggag cgaatcgtca
      601 agcacgagag gaacaagtcc gggtttcgtt tgttcgattc gcaagccaaa ctggttggcc
      661 atgcgatatc cgaaatccgt ggcgcccaaa ggtcgaatgg ataggccgcc cgtggcaatc
      721 acaagagacg agcattgaaa gactcccgaa tcagtttcga gcttgaatga tcccgaatga
      781 ctcacactcg atacgtgaca accggatttg attacaactc ccgtcgcaga gcattccgtc
      841 agcaacatgt caatgatgcc ttgcgacgac gagtcacaga atagctggcc caactttttc
      901 tcgtgaaatc tgatccggtg ttttttgacc agagaaataa aatcttcagc cgagtagcga
      961 gcgagtgcag actttgcgaa atgggggttt tgagaaagaa aggcatcggg tgttgtatga
     1021 atattcgtga aattgcagcg gccgccacct gatatctcga ttttccttcc aacgcgcgaa
     1081 ttgtgttcga ggacgagtgt tttgcgtttt cgttttccac tttcgattgc gcagaaaagt
     1141 ccggctgctc cacctcctat gattatgacg tcccacatga tgtgatgtgc acaaacaccc
     1201 gctaggggtt catcgttcgc tcgattcgtc ggtcaggtac gagccacatc accgcgatta
     1261 gaccgtaaaa caagcaggca atccacggcc gaacaaaagc aagtggcacc gcgatcacca
     1321 agatcaccat cgagatcttg cccttgaaat cattaccaat tgcgtccgcc agcacggaat
     1381 tgcgaccatg aagcgcgagc agcgtcttta caaggatgaa gtaggcaatg gctgccagga
     1441 ggagcacaat tccatagagt gccaccggcc acttagcaaa gtcgttttct cccatccacg
     1501 ccgtgacaaa gggaatcaaa gacagccaga agagcaggtg aaggttggcc cataaaacac
     1561 tcccattcac atgttcgact gcctgaagaa ggtggtgatg gttggtccaa tagattccca
     1621 aaaatacaaa gctcactaga tagctcagca gaatcggaat cagtggtcta agtccctgga
     1681 cggtggcatc gtgcggaggt ctcagctcga gcaccatgat tgttattaaa atcgcgatta
     1741 ctccatcgct aaatgcttcc atcctcgagg ttgtcatagt tcaaagccgg gctacttttt
     1801 tcggccaatc gcaaagagac tcgacgaaga ccgtacgtac attactcctt cagagatcgc
     1861 gggagtggcc atgagaagtt cacccatgga gttggtcgca aggtgcgcga attttggccc
     1921 agcgcttacc accagcattt ccccatcttc gtttgataag tagatctttc catcagccgc
     1981 aaccggcgaa gcactaaagc cacttcccac cagcggaagg cgttggcggt acagttcctc
     2041 tccggttttc agatcataag catcaaatag gccattgttt gccaatacat aaagtacgcc
     2101 cttataaatc aatggcgtgg gcatgtaaga gccgcggccg gttttactcc aaagcacagc
     2161 atcactacta gtcttgcctt cggggagagt taaatcgcct tgcgccccgg ccttgattac
     2221 aaagattggt cgttccggac cacgaccgct agcgacgacg agcacgtcat cagcataaat
     2281 tggtgtgggc gcggtgattt ttgagctacc gcccaggcgc cacagctctt taccggtctt
     2341 tgggtcatag ccgcgaatga agtttgacgc gttggcgata agctcgacgc ctttcgaagt
     2401 actggcgatg gtcggcgtgc cccatgaagg aatttcatca cgcttagttt tccagattgt
     2461 ttttcccgat tttgcatcga gcgcgataat gaacgagtcc gtttgggtat cgcattgcag
     2521 aataacgagg tcgttccaaa tgatcggtga actggccgat ccccattcga acgtgggaat
     2581 gtcgtaagcg cccagatcaa tgcggccgag atcgactttc catagaaacg ttccgctcac
     2641 gtcataagcg tacacaccct gggaaccaaa ccacgcaacc acgatccggc cgtctgtagc
     2701 tggagttgaa ttggcgtaag tggctttgat atgtcgtttc tcaattggct cgccctgata
     2761 tgccacgcgc tcccaggaaa tttttcccgt cgacttgtcg attgcataga tgacccaacg
     2821 atggaccgtg tggtctttag acgcatcgcc gtcgccgtag aggccagggc gaaagttcgc
     2881 ttttggatca ctgctaatcg ccgaagtgac gaacacggta ttgccccaaa caaccgggct
     2941 cgaatgagcg agtccgggaa ttaaggttcg ccacaaaata ttgttacccg ttttgggact
     3001 ccattcatcc gggaggtttt gtccctcgac aactccggat gcgtgaggtc cgcgaaatga
     3061 aggccaactg cctgaggagt ccgccggttt ggtactgctt cgcaacgtct taacggtagt
     3121 tacattgatt tgtcgtaccg gcttgtcaac tttctcgccc gagggaagcc aggtactgcg
     3181 gtcgagaatc atttgccgcg gttgacattc atcagaaacg agatcgaatg ttagacgatt
     3241 attattctta gcaagtttgt aacgtcctgg ctgatcacat ccgccgggcc ctccagacat
     3301 cgttaactga atctcggacc catctgtttt ccagttgccg gtcaacttgg gccatcgatc
     3361 accttccagc gtgaatgtac cgcccgcgtc gaaacgagca gtaaatactc caaacgacaa
     3421 tgggcctgaa ggcatttgga aatttgcttg ctgcgcgaac aaagtcaggc tcgcgtaaaa
     3481 aaggattgtt ccaactgtca gtgcaagctg gggagctttg ctaatcttca tattcttacc
     3541 tcgacactgg atcattgttt cttgccatac ttctcgatgt acttgcgggc aaactcgttc
     3601 aggtgaggac tatctttgag tccaaccttt tcggcttcag cttcagcgtc tttaatggac
     3661 caaccatcgc gcaaaacacg tctaatcatc caaaatgcac caactctaat gccggctgta
     3721 cagtgaatga atgcaggtcg attttcagga tcatctgtga tgcgtagaaa ctctgttgct
     3781 tgctcgtctt tcggatcgcc aaacacaaac ggaatattga agtagcgaag gcccagctct
     3841 tttgctttag cttcttcttc agctgcgcgg tgctcgctgg gttgtcgaag attgatgatt
     3901 gccttgacgc cttccgactt tagcttttcg agttgatcga gctgcggctg tccaccggtg
     3961 caaaactgat cattgacacg aaggaagttt cgaatgggcc gactttcttg aggagcttga
     4021 aacacggcga taagagtcat caacaagact aaaagcagat tattcatagg gtctccgaga
     4081 acgtccaggt gttgtgcgcc agcttacttc caaagaggca agttgaaaag ggggtaaacg
     4141 ccttatcaga ctatctatgg aaagttggaa ggcgagcaga gcggggtttc actcctactg
     4201 gagtgaacaa acctcgcgtc ttcacaagtg attgaatcgc agaaaacaaa aatccaaacg
     4261 tttgcaacgc agagagacgc tgagatatac caagggcagc caagttcggt agggcaactc
     4321 gagtgtccat tcgcaacttg caagttttta agactaatat tattcaagag ttaatttgcc
     4381 atgt
//
LOCUS       DASSFP010000082         4324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2100576, whole genome shotgun sequence.
ACCESSION   DASSFP010000082 DASSFP010000000
VERSION     DASSFP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100576"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1203)
                     /gene="lpxD"
                     /locus_tag="VFH91_01695"
     CDS             complement(157..1203)
                     /gene="lpxD"
                     /locus_tag="VFH91_01695"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET6861720.1"
                     /translation="MSKGKTTAEIAEYIQGRLVGDGSILIERIANLDTADLHELAYVD
                     NEKFFSVAANSGASCLLVPLDSAQTLKLSTAAIEVKNPKLAFALIAKLLHPPLHREPA
                     IHDTAVIAETADIALSAYVGPHASVGEYARIGNNSRVETGVVLGANVTIGDDCVIHPN
                     VVLYDGISIGNRVILHAGVCIGADGFGYVRDDMGYHKFPQIGTVEIEDDVEIGAHTCI
                     DRAALGKTRIGRGTKLDNMVHVGHNCDIGERVVIAAQTGISGSVVIEDDAVIGGQVGF
                     GDHTRVQKGAIIGSKAGILPGKIVRPGVWWGIPIQPLDEYKRMNAHIGRLPQMREELK
                     ELKARVKELEDRLS"
     gene            complement(1278..2087)
                     /gene="lpxA"
                     /locus_tag="VFH91_01700"
     CDS             complement(1278..2087)
                     /gene="lpxA"
                     /locus_tag="VFH91_01700"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET6861721.1"
                     /translation="MRIHESAIISASARLGNGISIGPYAVIEDDVELGDECEIAAHAV
                     IKKFTKLGRRNRVFEHAVLGGEPQDVKFKNERSYLEVGDENLIREFCTLHRASGTDEI
                     TRVGSRNFFMIGVHIAHNCDIGDDNIFANNAALAGHIKVEDHVFLSNNAGAHQFVQMG
                     RYAMVGGKSKIVQDVLPFFVSDGNPARVRGLNNVGLRRAGLSTESRLQLKRAYRLLFR
                     SNLQLSDALEQMSALMDPNVDHLVQFIRNSKRGFTHTERRDQKNASQDETD"
     gene            2501..>4324
                     /locus_tag="VFH91_01705"
     CDS             2501..>4324
                     /locus_tag="VFH91_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6861722.1"
                     /translation="MFSDQFRRGESDKSKLSGVKGSKLISGLSDVAWKAFQSVNQLLP
                     EGEAIRPNWAPGPLLKSYERTAPPLGFPRETDSLCPSCVKEVRNAVITGQVSLETLMN
                     EHPGEIKAQILEANGQVVMRKTCPKHGEFEDVMSTDPAFLNRIESLFFGRDFRAAEDK
                     DVHRHGTSNIQFGRGAVLTVDLTNRCNMMCNPCFMDANQVGYVHEPTFEDTKALLDRA
                     VSFKPRRQVIILFSGGEPTLSPYYLDAVAYAKKIGFYRILAATNGIRFAEDLEFCKAA
                     KAAGQHGVYLQFDGVSEEKNKHRGVGNLFDVKLQAIENLAKVGIKVTLVVTIVNNINN
                     DAIGQIVEFAAKNIDKVQTIAFQPVSFTGRDEDISDEMRTKWRYTLAGMTHDLKDQLD
                     GRIQPLRDWFPLSSYSAFTSVMDMLQGADAPWGWSSCNCHPNCGIFTLQVVNRHTGEF
                     KSLFEFFDYEQFMKDVAVITDTARGKKLSYAQLGMAIMRNFDAERAPEGFPISQIINL
                     FKPSSANSNSDRNDRMKAQTGANENDVWRVLCVEGMWFQDLFNYDFRRTEMCVIPYGT
                     QEGEISFCAYNTGVGWRQIIENMHKTANLAEWYEDNGRHKVY"
BASE COUNT         1082 a         1114 c         1044 g         1084 t
ORIGIN      
        1 aaagagataa ttgaattgtt ggatcctgaa cgaaacgaaa aggtgcgtta tcagttacga
       61 cagatcgcga atcgactggg ggaatcagaa gcctctgatc gcgcggccag gtcaatactg
      121 aaattcctgc gcgtggatgg atgatgcacg taaaaattaa ctgagcctgt cttcgagttc
      181 tttaacgcgg gccttgagtt cttttaattc ttcacgcatt tgcggcagtc gtccaatatg
      241 ggcattcatc ctcttgtact catcgagcgg ctgaatagga atgccccacc aaacacctgg
      301 tctgacaatc tttccgggaa gaattcctgc ctttgaccct atgattgcgc ctttttggac
      361 tcgagtatga tctccaaacc cgacctgccc tccgataaca gcatcatctt cgatcactac
      421 acttccgctg atgccggttt gcgccgcaat aacaacgcgc tcgccgatgt cgcagttatg
      481 gcccacgtgg accatgttgt ccagttttgt gccacggccg atacgagttt tgcctaacgc
      541 ggcccgatcg atacatgtat gcgcgccgat ttccacatca tcctcgattt caacagtacc
      601 tatctgtggg aatttgtggt aacccatgtc atctcgtaca taaccaaagc cgtccgctcc
      661 gatacaaacg cctgcatgga ggattacccg gttgccgatt gatatgccgt catacaaaac
      721 tacattgggg tgaattacac agtcgtcgcc gattgtgacg tttgcaccaa gcacaacacc
      781 tgtttccact cgagagttgt tgccgattcg agcatattct ccaaccgatg catgtggtcc
      841 aacatacgct gagagggcta tgtctgcagt ttcagctatc acggctgtat cgtgaatggc
      901 cggttctcga tgaagaggcg ggtgaaggag tttggcaatc agggcaaatg caagttttgg
      961 attcttcacc tcaattgctg ctgtactgag tttcaaagtt tgtgcggagt cgagcgggac
     1021 cagcaaacat gaggcgccac tattagccgc aacactgaag aacttttcat tgtcaacata
     1081 agcgagttcg tggaggtcgg cagtgtcaag attcgctata cgttcaatca ggatggatcc
     1141 atctccgacc aggcgcccct ggatgtactc agcaatttca gcagtcgttt tgcctttgct
     1201 cattgatcac ttcttccatt ttgagttcat gaggagcacg tttatttaat actgagagga
     1261 cagatttcta aattccatca atcggtttcg tcttgagaag catttttctg gtcacgcctt
     1321 tccgtgtgcg tgaatccacg tttcgagttg cgaataaact gaacgagatg gtccacattc
     1381 ggatccatta acgcgctcat ctgttccaaa gcgtcggaca gttgcaaatt cgatcggaac
     1441 aacagacggt atgcgcgttt tagctgcagt cgactttcag tggacaagcc cgctcgtctc
     1501 agtccaacgt tgtttagacc tcgcactctg gccggatttc cgtcggatac aaagaacggt
     1561 aggacatcct ggactatctt agacttgcca ccgaccattg cataacgacc catttgcaca
     1621 aattgatgag ctcccgcgtt atttgataga aaaacgtggt cctcaacctt gatgtgtccc
     1681 gccagggcgg cgttattcgc aaatatgttg tcgtcgccaa tgtcgcagtt gtgagcgata
     1741 tgaactccaa tcatgaagaa atttctggac cccactcgag taatttcgtc ggttccactg
     1801 gcgcgatgca gagtacaaaa ctcgcgaatc aagttctcat caccgacttc gagataactt
     1861 cgctcatttt tgaactttac gtcttgaggc tcgcccccta acactgcatg ttcaaaaact
     1921 ctgttgcgcc ggcccagctt tgtaaacttc ttgatgacag catgagccgc tatttcgcac
     1981 tcgtcaccga gctcaacgtc atcctcaatc accgcgtaag gcccgatact gatgccattg
     2041 ccgagcctgg cagaagcact aataatcgca gattcgtgaa ttctcatttg ttcaaagggt
     2101 agcctggcgt catccttcag aaggcgcttc cccggccggt tacactcgcg accgcgggaa
     2161 gtcaggtcag gatataccac acgccgcccg ggctaaccca gtccctgaag aacccgagaa
     2221 agaccgaatt aggcacgcga ttacgactaa accctttgtt aatggaggtc atccaagttt
     2281 gcgatggatg gaagaaccgg tcgcgggccc cctctttgca agacgatcat cagattttcg
     2341 agcttttttc cgcttgccat cgcggtttag gccaactatc atgcttccgc cgctttttgg
     2401 ttggccgtca acacaaatta agcggtttat ttttgtccta gttaggctta atcggcatcc
     2461 aagccgtctt agttagcgtt cgcaacagga gatagaacct ttgtttagtg accagtttcg
     2521 cagaggagag tcggataaga gcaagctctc tggagtaaag ggctctaagc tcatttccgg
     2581 tctctcagac gtagcctgga aagcttttca atcggtcaat caacttcttc ccgaaggcga
     2641 ggccatccgt cccaactggg cgcctggtcc acttctaaaa tcctacgagc gaacggctcc
     2701 accactcggc ttcccaagag agacggacag cctgtgtcca tcttgcgtta aggaagttcg
     2761 caatgccgtc atcactggtc aagtgtcact tgaaaccttg atgaacgagc atccaggtga
     2821 aatcaaagct caaatcctgg aagccaatgg acaagtcgtg atgcgcaaaa cttgtccaaa
     2881 gcatggtgag ttcgaagatg taatgtccac tgacccggca tttctgaacc ggatcgaatc
     2941 gttatttttt ggtcgcgatt ttcgggccgc cgaagataaa gacgttcata gacatgggac
     3001 gtcgaacatt caatttggac ggggcgcggt attgacagtc gatctaacca atcgttgcaa
     3061 catgatgtgc aatccgtgct tcatggacgc gaatcaggtt ggctatgttc acgaaccaac
     3121 tttcgaagat acgaaagcct tattggaccg ggctgtctca ttcaaaccac gcaggcaagt
     3181 aatcatccta ttctccggcg gtgaaccaac actctcgcct tactacctcg atgcggtagc
     3241 ctacgcgaaa aagattggat tctatcgaat actcgctgcg acgaacggaa ttcgtttcgc
     3301 cgaagatctc gagttctgca aagctgccaa ggctgcgggc cagcacggcg tgtacctaca
     3361 gtttgatggt gtcagcgaag agaagaataa acaccgcggc gtaggaaatc ttttcgacgt
     3421 caaacttcaa gcaattgaaa acctggcaaa ggttggaatt aaagtcacgc ttgttgtgac
     3481 aatcgtgaat aacatcaaca acgacgcgat cggacagatc gtggagtttg ctgccaagaa
     3541 catcgacaaa gttcaaacga tagcgtttca accagtatcg ttcaccggac gcgacgaaga
     3601 catttcggac gaaatgcgca cgaaatggcg ctatacgctc gcaggaatga ctcacgatct
     3661 gaaggaccag cttgatggtc gtattcagcc cctgcgcgat tggtttccgc tgtcttccta
     3721 ctcagctttc acgagcgtta tggacatgct ccagggcgcc gatgctccat ggggctggtc
     3781 ctcatgtaac tgtcacccaa attgcggcat cttcactttg caagtggtca atcggcacac
     3841 cggcgaattc aagtcactgt tcgagttttt cgactatgaa cagtttatga aagacgtggc
     3901 ggtaattacg gacactgcgc gtggcaagaa gctctcttat gcccagttgg gaatggcaat
     3961 catgcgaaac ttcgacgccg aacgggcgcc tgaaggtttc ccaatttcac agattatcaa
     4021 cttgttcaaa ccatcctcgg caaactcgaa ctccgatcgc aacgaccgca tgaaggccca
     4081 gacaggcgca aacgagaacg atgtctggcg cgtactctgc gttgagggga tgtggttcca
     4141 ggacctcttc aattacgatt ttcgacgtac cgaaatgtgc gtaattcctt acggcactca
     4201 ggaaggtgag atctcattct gtgcctacaa cacaggagtt ggctggcggc agataatcga
     4261 gaacatgcac aagaccgcta acctggcaga atggtacgaa gataatggcc ggcataaggt
     4321 ttac
//
LOCUS       DASSFP010000083         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1656743, whole genome shotgun sequence.
ACCESSION   DASSFP010000083 DASSFP010000000
VERSION     DASSFP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656743"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFH91_01710"
     CDS             complement(<1..878)
                     /locus_tag="VFH91_01710"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6861723.1"
                     /translation="MSLTKRFLLVLLLIVAVGLVYLLVAPVPINPAAWTPPIAPTLTG
                     QYQQNNRLASTERLPLGDGHGPEDVALDADSKIYAGFDDGRIIQLQADGTQPRVFANT
                     AGRPLGLIFDSTGNLIVADAVKGLLQIDRDGKITTLVDQTNGVRFGCLNDLDVGKDGT
                     IYFTEASNKFPMTQFTEDLLEHQPNGRLMAFDPKTHQTRTLLPGIHFANGVAVSPDQN
                     SVLVNETGMYRVLRFWLRGEKAGRSEVFIDNLPGFPDGISSNGNDKYWLSLVTPRDSF
                     FDRILPHPFLRKMVMR"
     gene            complement(891..1694)
                     /locus_tag="VFH91_01715"
     CDS             complement(891..1694)
                     /locus_tag="VFH91_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HET6861724.1"
                     /translation="MEIIEDIDDWRLRFSSWKPAGNEDDLGPDYPFVKNQRAPFTPAR
                     RALPMLNLALISSAGAYIDGTQGFDIRSPLGDLSFREIPIEVDGLALKFAARGYDPAA
                     VEADANSQLPVERLLEFESNGIIGQLNSVFWSFSGYIPDAAKLADELAPRIVERLQRY
                     EVQAALLVPASRLCHQSIGLVARAIEDAGIPTMSLAVLKDIVEKVRPPRVALYNGELG
                     SVAGLPNWPEHQRRILDEALRLIEPMDQPGIRKLVVALESEVENARGER"
     gene            complement(1722..2195)
                     /locus_tag="VFH91_01720"
     CDS             complement(1722..2195)
                     /locus_tag="VFH91_01720"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HET6861725.1"
                     /translation="MILVACWLGAALFFSVVVAPAAFGVLRSFALANASEIAGAIVNR
                     SLGVVNVSGFLIGLIAFFTGLLLRSSSSRGLWFVVQSISLLILTIATAVGHWVIAARI
                     HTLRTASQLRIDQLAANDPTKIAFDSLHGYSVQALGLAMIAALVALFAIARSFKN"
     gene            complement(2228..2659)
                     /locus_tag="VFH91_01725"
     CDS             complement(2228..2659)
                     /locus_tag="VFH91_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET6861726.1"
                     /translation="MNSNQPHYTEAHIIAYPVEYIAGIDLYNAGEFHAAHDAWEERWM
                     GEVGPREKLFLQAMIQSAVAFHHLDIGRPGAARQMYVRSKEKFAALGHEVFMSLDLVE
                     YQAQLDSALSWLLSVADPREVPVPEFVPPTIRLVAAIDTFD"
     gene            complement(2661..3590)
                     /locus_tag="VFH91_01730"
     CDS             complement(2661..3590)
                     /locus_tag="VFH91_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6861727.1"
                     /translation="MYDVVIIGAGPAGLSAAFWCDELGLDTLLLEQHDRVGGQLWHVY
                     NKIENYPGLKTENGEELARLFSRDVEAAGFDLWTQVEIESVDLKARRVSLRSGEQLST
                     ISIIIATGVRRRVLGIPGEQEFAGKGMIESASRDREKFTGRDVCVVGGGDAAAENALA
                     LAEVCPTVTLVHRGKTLRARREFVEKLQTDHRITVFTESQVTRIMGQDHLEAVEIKRK
                     EGLKPFQIAVGGLLVRIGVEPNTELFKDQLDLDEAGYIRVQSTQETSQPFVFAIGDVA
                     NPLAPTVSGATGSGATAAKVIAARLNPTAKRSS"
     gene            complement(3597..4577)
                     /locus_tag="VFH91_01735"
     CDS             complement(3597..4577)
                     /locus_tag="VFH91_01735"
                     /inference="COORDINATES: protein motif:HMM:NF042562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6687 family protein"
                     /protein_id="HET6861728.1"
                     /translation="MRFEFYSEAIANTPKLSVDGTVANSVHFSHWNGNETPPEVKADT
                     STEIALNLVASPNKPTLTRGIELVTNNHFDTDGVLSVWTVLNGERALKFRDLLIAAAE
                     AGDFSEFSSKDGICVSLAIQGNDQAFPNEDTASPLARSVAKEAVTDDARSYDLVLPQV
                     ESLLTNPAAFESLWLEGWSRISAAMKSFESAKSRVQELGGTGVTLITLAQELFGENGF
                     NPTRHLVPFTAISKFANGELFVVAVPSNNGWCYRIDYPYYSWAETVVRPRVARRDLSG
                     VVESLNRRERNSGGRWQTDDRELTSAVKFVDENGRLVVSGLMPEEVVSDF"
     gene            4784..>5047
                     /locus_tag="VFH91_01740"
     CDS             4784..>5047
                     /locus_tag="VFH91_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6861729.1"
                     /translation="MEEISSMELKNRLDAGDDIQIIDVREDNEVALGTIPNAKHIPLA
                     QVLARMEEIEPDHDTVVYCKMGGRSARAIDALQRSGFTGKLINL"
BASE COUNT         1295 a         1375 c         1205 g         1172 t
ORIGIN      
        1 aatcgcatca ccatctttct taagaacgga tgcggcagga tgcgatcaaa aaacgagtcc
       61 ctcggcgtta ccaacgacaa ccagtatttg tcgtttccat ttgacgagat gccgtctgga
      121 aagccgggca agttatcgat aaaaacctcc gaccgtccgg ccttctcccc acgcaaccag
      181 aaacgaagta cgcgatacat tccagtctcg ttcacgagca ctgaattttg atccggactg
      241 actgcgacac cgttggcaaa atggattccg ggtaataagg ttcgggtctg atgggtctta
      301 gggtcaaacg ccattaagcg accgttgggc tggtgctcga gcaaatcttc cgtgaactgc
      361 gtcatcggaa atttattcga agcttcagtg aagtagattg ttccatcttt cccaacgtcc
      421 aggtcgttca aacatccgaa ccgcacgcca ttcgtttgat ctacgagagt tgtaatcttg
      481 ccgtcccgat cgatctgcag gagaccctta actgcatcgg cgacaatcag gtttccggtt
      541 gaatcaaata tcaggccaag cggccgacca gcagtattgg caaagactct cggctgtgta
      601 ccgtccgcct gaagttggat gatccgaccg tcatcgaatc ctgcataaat tttagagtca
      661 gcgtcgagag ccacatcctc tggaccatga ccatccccaa gtggaagtcg ttccgtcgag
      721 gccagacgat tgttctgctg atattggccc gtaagagttg gcgcaatcgg aggcgtccac
      781 gcagctggat taatgggaac gggcgcaacc agtagataca ctagccccac ggctacaatg
      841 aggagtaaca ccagtaagaa acgttttgta agggacatag ttgagtggtc ttagcgttcg
      901 ccacgcgcgt tttcaacttc ggattccaat gccactacca actttctaat tcccggttgg
      961 tccatcggct cgatcagccg cagggcttcg tccaatattc tccgttgatg ttccggccag
     1021 ttgggaagac cagcgactga cccaagttcg ccgttataga gggcaacgcg cggtggccga
     1081 actttctcga cgatgtcttt taagactgcc agactcatag ttggtatccc cgcatcttcg
     1141 atcgcacgag ccaccaatcc aatcgattga tgacaaaggc gtgaggcggg aaccaacaaa
     1201 gccgcctgca cttcatacct ttggagtcgt tccacgatcc tgggtgctaa ttcatcagcg
     1261 agttttgctg cgtccggtat gtagcctgag aaactccaga agacggagtt cagttgacct
     1321 atgatgccgt tactttcgaa ctccaacaga cgctcaacgg gcaattgcga gtttgcgtcc
     1381 gcttcgaccg cggctggatc gtatccacgg gccgcgaact tcaaagccag gccatcaacc
     1441 tcgatgggga tttccctaaa cgatagatct cctaacggcg accggatatc aaatccctgg
     1501 gtgccgtcaa tgtaagcgcc ggccgatgaa atgagggcca ggttgagcat cggtaatgcg
     1561 cgtcgcgccg gtgtaaacgg cgcccgctga ttttttacaa acggataatc cggtcccaga
     1621 tcgtcttcat taccggccgg tttccaggaa ctaaagcgca gccgccaatc atcaatatct
     1681 tcaattatct ccacaactac tccctaacct tttcagataa ttcagttttt aaaacttcga
     1741 gcaatagcaa ataacgctac aagggcggca atcatagcaa gaccaagtgc ctgcaccgaa
     1801 tagccgtgca gactatcgaa tgcaatcttc gttgggtcat tggccgctag ttggtcgatc
     1861 ctcaattggc ttgcagtccg caaagtgtga attcgtgccg caataaccca atgtccaaca
     1921 gcagtagcaa tggtaagaat cagcagtgag atggattgca ccacaaacca caatccgcgc
     1981 gacgaactac ttcggagcag tagaccggta aaaaaggcaa tcagaccaat cagaaaccca
     2041 ctcacattga ctacaccgag tgatcgattg acgatcgctc cggcaatctc actcgcattg
     2101 gcaagagcga aggatcgtag tactccaaat gctgccggcg ctacaacaac actgaaaaag
     2161 agtgcggcac ctaaccagca agctactaaa atcatccgaa tggaattgat gatgagccga
     2221 actcctctca atcaaacgta tcgatcgcgg caaccaaacg gatcgtaggc ggcacaaact
     2281 ctgggaccgg gacttctctc ggatctgcga cactcaataa ccatgacaaa gctgagtcga
     2341 gttgcgcctg atattcaaca aggtcgagtg acataaagac ttcatggccc aaagcggcga
     2401 atttctcttt agacctgacg tacatctgac gcgctgcgcc cggccggccg atatccagat
     2461 ggtggaaagc aaccgccgac tggatcattg cctgaagaaa taacttttcc ctgggaccaa
     2521 cttctcccat ccaccgctct tcccacgcat catgtgctgc gtgaaactct ccagcgttgt
     2581 acaggtcgat tcctgcaatg tactcaactg gataagcgat gatatgcgct tcagtatagt
     2641 ggggctgatt cgagttcatt tcaactcgac cttttggcag tggggttcaa tcgagccgcg
     2701 ataactttgg cggccgttgc accacttccg gtcgctccgc taacagttgg ggccagcggg
     2761 ttggctacat cccctatcgc aaaaacaaag ggctgactgg tttcctgcgt actctggacc
     2821 cgaatatatc ccgcttcatc caggtcaagc tgatccttga acaactcagt gttaggctca
     2881 acgccgatac gcacaagtaa gccgccaaca gcgatttgaa aaggcttcag gccttccttg
     2941 cgttttatct ccactgcttc caaatgatct tggcccataa tacgcgttac ctgagattca
     3001 gtaaaaacag tgatgcgatg atcggtttgg agcttctcaa caaactcgcg gcgcgcccgc
     3061 aacgtttttc ctcgatggac caaagtcacc gtcggacaca cttcagccaa cgctaaagca
     3121 ttttcagcgg ccgcgtcgcc tccacctact acgcaaacat ctcgtccggt gaacttctcc
     3181 cgatcccgag aggctgattc gatcattccc tttccggcaa attcttgttc gccgggaata
     3241 cccaggactc ttcgtctgac gccggtggcg atgatgatcg aaattgtgct caattgctcg
     3301 ccactgcgaa gcgagactct tctggctttg agatcaacgg attcgatctc aacttgcgtc
     3361 caaagatcga accccgcagc ctcgacatca cgcgaaaaaa gtcgagccaa ttcttcaccg
     3421 ttttccgtct ttagaccagg atagttttca attttgttgt agacatgcca caactgccca
     3481 cccacccgat cgtgctgctc aagtagaagg gtgtccagac caagttcatc acaccaaaaa
     3541 gcggcggata gaccagctgg gccagctccg atgattacga cgtcgtacat aagctcctag
     3601 aagtcggaca caacttcctc gggcatcagt cccgagacaa ccagccgtcc attttcatcg
     3661 acaaacttga ctgccgaagt cagctcccga tcatcggtct gccatcgacc acccgaattt
     3721 cgttctctcc gattcaaact ttccaccact cccgagagat cccgacgggc cactcgaggc
     3781 cgaacgacag tttcggccca tgagtagtaa ggataatcaa ttcggtagca ccagccatta
     3841 ttcgaaggca ccgcgacgac aaaaagttcc ccattagcga attttgagat cgcggtaaat
     3901 ggcaccaggt gtctggtcgg attgaaacca ttctcgccga aaagttcctg ggccaatgta
     3961 atcaacgtaa ctccagttcc acctagttct tgaactcgag actttgctga ttcgaagctc
     4021 ttcatagcgg ccgaaattcg gctccagcct tcaagccata atgactcgaa agccgcagga
     4081 tttgtcagta gcgattcgac ttgtgggagt acaagatcgt atgagcgcgc gtcgtcagtc
     4141 accgcttctt tcgcaaccga tcgagcaagc ggagacgcag tatcttcatt tgggaatgct
     4201 tgatcgttac cttgaattgc gaggctgacg cagataccgt cttttgatga gaactcggaa
     4261 aagtcgcctg cttcagcggc ggcaatgagc aagtcgcgaa attttagagc acgctcgcca
     4321 ttcaaaacgg tccagaccga gagtacaccg tcagtgtcga agtgattgtt tgtaactaac
     4381 tcaatacctc gtgtaagagt cggcttgttc ggcgaagcaa ctagattaag tgctatctcc
     4441 gttgaagtgt ctgccttcac ttcaggtggg gtttcgttgc cgttccaatg ggaaaaatgg
     4501 acactattgg caaccgttcc atcgaccgac aacttcggag tgtttgcgat ggcctcgctg
     4561 taaaactcga atctcatgtt agacctcttt ggagtgcgcc ggcccggcgg cgctttgtta
     4621 ttacagacga tttcctatgt tactaaagac gtatccactt gggagactac cccggaatac
     4681 caaagcgccg gcgggccggc gcactccaag ggatttgact tgattcggct acttaagttt
     4741 aggatacatc aatcaaacaa aacttagata acaaggagcc gcgatggaag aaatcagctc
     4801 aatggagctc aaaaacagac ttgatgccgg tgatgacatt caaatcattg atgtgcgaga
     4861 agacaatgaa gtagcacttg ggacgatccc taatgccaaa catattcctc tagcccaggt
     4921 acttgcgcga atggaggaga tagaaccgga tcacgataca gtcgtctatt gcaagatggg
     4981 tggacgaagt gctcgtgcaa tcgacgctct tcaacgttcg ggatttaccg gcaaactcat
     5041 caattta
//
LOCUS       DASSFP010000084         9975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1505476, whole genome shotgun sequence.
ACCESSION   DASSFP010000084 DASSFP010000000
VERSION     DASSFP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505476"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFH91_01745"
     CDS             complement(<1..190)
                     /locus_tag="VFH91_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861730.1"
                     /translation="MSLLSLRVKRGLKKALPYLQLLEIYHRFRNRNTLTIVVFHRVLA
                     VDDDRWVDAHPEWVVSDVV"
     gene            complement(205..1446)
                     /locus_tag="VFH91_01750"
     CDS             complement(205..1446)
                     /locus_tag="VFH91_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6861731.1"
                     /translation="MIDLSFSRVSKRYRIRHESEESSSRHPLIRKWNALWTRPEDFWA
                     VRDVSFEVERGEALGIIGHNGAGKSTILKLLSNITTPTDGEIRINGRLAALIEVGSGF
                     HPELTGRENIYLNGAILGMARNEITKKLDSIIEFAGVRQFIDTPVKRFSSGMYVRLGF
                     SIAAHLEPDILLLDEVLAVGDASFQKKCLERISELQEAGTTIVFISHDLRAVERLCQR
                     VLLLKRGQIVMNGAARDVVAAYQNDATLSSESVEVHKKGETKEVEILTLSFLNGAREF
                     SPVFKTGEPLIAKIDFEVHQPVSDAVFSILLFTSDGESHCELTTEFSRERLSLERGKG
                     SIEFRTNELGLLPGIYFTSVQVQQRDARDVMHYQYKSTTLRVDFDRVIRGNFFLPHEW
                     SIKHLRKGDESQEEVAMGCDR"
     gene            complement(1471..2346)
                     /locus_tag="VFH91_01755"
     CDS             complement(1471..2346)
                     /locus_tag="VFH91_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6861732.1"
                     /translation="MVEIATAHNSSTVARRRPVKIIRPASFSPSGFLRDFKGLWLYRD
                     LLITLSAHRVKVRYKQSVLGIAWAILQPLSLMLIYTLVFAVFARIPTGGTPYPVMAFT
                     GLLLWTLFSTALTSSTSGLVSHAHLVTKVYFPREILPLTYVVAALFDFAIASLLLAAL
                     MVYYHVAPTINLLYAIPIVVILTCFVTAMSLVFSAVQVRFRDVGVAMPLLLQLWMFAT
                     PVVYPLSAVPQRFQSLYSMNPMVGVVDSFRTVVLNGTAPDLHALGISAIVSILLLPAC
                     YIYFKHVEATLADVI"
     gene            complement(2358..3548)
                     /locus_tag="VFH91_01760"
     CDS             complement(2358..3548)
                     /locus_tag="VFH91_01760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6861733.1"
                     /translation="MPTNRGHIAHVLPWSTIGGTEQATLRLAQAVGNDNFVHTAFCQG
                     TDSPVRDLFSSARFDTARFDAIEPSYKHPSAYVRASLRLAREFRRRRVDLVHCSDLLA
                     AYYAGLAGKLARLPVLCHVRCAYPEISRRDQSFLKVVNHFAFVSNDAWHSFAFKVPAA
                     RGTVIYDGIDIKQPRGNLDQSVREEFEVPQGASMVGMIARVAPAKDFATLIRAAKRVV
                     SKFPEARFLIVGDYSQVQINRDHFNEIQPLLAAAGLLHNFIFTDHRNDVDRLLAAMDL
                     FVLSTKTEGLPLVILEAMAQGKAVLATAVGGVPEVIKDGETGLLHRAGDDEQLASQIL
                     SLLEDQGQRERLGDAGREAVKMNFTREQFGRRMAELYGEMIGRPQLITAGFEKTNERR
                     ELTS"
     gene            complement(3549..5759)
                     /locus_tag="VFH91_01765"
     CDS             complement(3549..5759)
                     /locus_tag="VFH91_01765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6861734.1"
                     /translation="MSFTRVEFIFQLIFWSGFVALLYSYVGYAIVLVLLSRLRGRGVK
                     SEDFTPTISFIITAYNEERDLGAKLRNTLALDYPKDKFEIIVASDCSSDRTDEIARSF
                     ANEGVRLHRQLERLGKTAAQNRAVELATGDVILFSDATTLYRPDVLRVISRNFADETI
                     GCVAGQLIYVDPSNSSVGKGAVSYWNYEVFLKRHESNVRSLIGVSGCLYAVRRSAYVP
                     LYHEACSDFIIATKMVEQGLRTIYEPDAICEEETNSRADKEFRMRVRVITQTYNDLWR
                     HRHIFNPFRYGVYALQLFSHKVMRYLVPVFLAMLFISSLVLATRSLFFAFVLVLQLAG
                     YAAALVGYLLERTGLRGGLISLPHYFLLANLAAVVAFYEFVRGERYAKWEPIRQTGPA
                     GPEVPRASKEEVASVATLSSLPGAPQVTVIIPAYNAAGFIEDAVSSVLAQTFSDYELI
                     VVNDGSPDSEELARVLSPYSDRIIYLEQQNRGPSGARNAAIEIAKGKYLALLDSDDTW
                     LPNYLTEQMKFLNQVPETDMVYADALLVGEGPLVGKSFMQSSPSIGPVTFESLLRYES
                     SVITSCSVINREKVIAAGLFDERFVRCEDFDLWIRLAHQGARIRYQTQVLARHRISDA
                     SLAADQIRMVESQIEVLKKAQQTLPLTPEQLKLVDSQLANCTAQIDLAQGKRFFDVHD
                     YEQAAQRLRTANNFYKSRKLQLIVWGLQSAPWFLSSLNWMRQNLWSPASKTVSK"
     gene            complement(5785..7233)
                     /locus_tag="VFH91_01770"
     CDS             complement(5785..7233)
                     /locus_tag="VFH91_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6861735.1"
                     /translation="MPKRYEFQDYDPLFRTPGNRGGEGLVEEPWPVRGAEPEASSQGE
                     SLFTNLSQPPTTESLPKPESTVRLILKRGHVVSYAGLFLFTIVLYFRPYELFAVLSWM
                     DKVAYWLAIGTIIIFLPSQLVLEGNLSARPRVVGLVLLLTLAGLFSIPTAINPLEAWN
                     EFNDVFIKAVLMFIVMINVVRTERRLMALIYVALAVTFVLSFNAIRDYQTANFAVEGY
                     RIKGLLGGMFGNPNDLALHFVTMTPIAITMAFATRKLILKILLIITAILIVAGNVVTY
                     SRGGFLGLLVVAVVVAWKIGRKRRLLLAIVGPIVLTLFVLLTPGSYGIRLLSIFIPGL
                     DPVGSAGSRQEILNRSIIVSLRNPITGIGMGNFHIMSLHETVTHNAFTQVSAEMGFGA
                     LIVYLMFLTAPILWLRKLERETSTESRASFYYYLSIGLQASLIGYMVSAFFAAVAYQW
                     YAYYLVGYGVCLVRIYQSKTSSDLNSLTRAQG"
     gene            complement(7409..8608)
                     /locus_tag="VFH91_01775"
     CDS             complement(7409..8608)
                     /locus_tag="VFH91_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861736.1"
                     /translation="MKSISEDYEQVLEPGAEAKPPQQPRVRRSAERNAKNAKWVESLR
                     SSDVNRLWSELHRIVSHHPLVRASRSAGLLIEEGGGSAYTDLTQELFVQLLSKNRFEH
                     YLETAMTDYEIECEISQIELTNLLTAELRKRHPESYRLARRISTLIQSSTNFRRFDSS
                     GSEDEPHRRLADRVYGLSEWNEKKSRRGAHEVEQRVQIIPVRQRDTRMVGCTGDSQVV
                     ITNPDLEDLIVSVLDAVDSPVDVRTLRSLVMSRLPVMDIYLVPLDGDDSDNENSNAYE
                     PVDKRENPEEGLLKRESEREAAGSVDLFLKNLNEAVRGKVKQYNRILGVLWYCYLSPD
                     HSTQLEVAAALGVSDSLVSDYRRRIEHELRTLSFTEVEEAKHFEMALRERVRACMPAI
                     ANSELTV"
     gene            complement(9479..9859)
                     /gene="crcB"
                     /locus_tag="VFH91_01780"
     CDS             complement(9479..9859)
                     /gene="crcB"
                     /locus_tag="VFH91_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET6861737.1"
                     /translation="MQRLLLIGFAGLAGTLGRYFMSVLLARRFGESFPVGTIIINVLG
                     CFLAGFLFYVMQERSLVSETVRLAIMVGFLGGFTTFSAFGLQTFTLFRDGELFLALLN
                     LAVSNVAGLLTVWVGFLLARAVMS"
BASE COUNT         2688 a         2580 c         2357 g         2350 t
ORIGIN      
        1 aaacaacatc tgatacgacc cactccggat gggcatcgac ccagcgatcg tcgtcaaccg
       61 ccagtactct atgaaaaacg actatcgtca gtgtattgcg attgcgaaag cgatgataaa
      121 tctcaagcaa ctgtaaataa ggcagtgctt tttttagtcc tctttttact cgtagactta
      181 ataaagacat aaaacatcac accctcaacg gtcgcagccc attgcgactt cttcctggct
      241 ttcgtcacct tttctcaaat gcttgatact ccattcgtgc ggcaagaaga agttgcccct
      301 gataacccgg tcaaaatcca cccgcaacgt cgtcgacttg tactgatagt gcattacatc
      361 acgtgcatca cgttgctgga cctgaacaga tgtgaagtag atgcccggca ataaaccaag
      421 ttcattggtg cgaaactcaa tcgacccctt gccccgctca agcgagagtc tttctcgact
      481 gaactcagtt gtcagttcac agtgcgactc gccgtcggat gtaaacagca gtattgagaa
      541 aactgcatcg cttacaggct gatggacttc gaagtcaatc tttgcgatca agggttcacc
      601 cgtcttaaat accgggctaa actcgcgcgc gccattgaga aacgataacg tgagaatctc
      661 aacttcttta gtctcacctt ttttgtgtac ttccaccgat tcgctcgaga gggtcgcatc
      721 gttttgataa gctgcgacaa catcccttgc ggcgccattc ataacaatct ggccgcgctt
      781 tagtaacagc actctttgac acaatcgttc tactgcacga aggtcgtgag aaataaaaac
      841 tatggttgtg cccgcctctt gcaattcaga gatacgttca aggcatttct tttgaaatga
      901 cgcgtcacca acggccagca cttcgtcgag tagcagaatg tcaggctcga gatgggccgc
      961 aatcgaaaac cctaatctga cgtacatccc tgacgaaaat ctcttaaccg gcgtatcaat
     1021 aaactgtcgc acacccgcaa actcgataat actgtcgagc ttctttgtga tctcgttgcg
     1081 cgccataccc agaatcgcgc cattcagata aatattttca cgcccggtaa gctccggatg
     1141 aaaaccagag ccgacctcaa tcaatgccgc aagtctgccg tttattctta tttcgccatc
     1201 agtaggtgtg gtgatgttcg aaaggagttt caggatcgta ctcttgcctg cgccattgtg
     1261 gccgataatg cccagtgcct caccgcgctc aacttcaaag ctgacatcac gaacagccca
     1321 aaaatcttcg ggtctcgtcc acagcgcatt ccatttacgg ataagcggat gtcgagacga
     1381 gctctcttcg ctctcgtgac ggattcgata tctcttagag actcggctaa aactaagatc
     1441 gatcatcaat tcggcttgac ctcagacgcg ttagataaca tctgccagag tggcctcaac
     1501 gtgcttgaag taaatgtagc aggctggcaa taacaagatg gacactatgg cggaaatgcc
     1561 cagggcatga aggtccggag cagtcccatt caaaactacc gttctaaaac tatcaaccac
     1621 acctaccatc ggattcattg aatacagact ttgaaatcgc tgtggtacag cgctaagagg
     1681 atagaccaca ggagtcgcaa acatccacaa ttgcagaagc aaaggcatcg ccactcctac
     1741 gtcgcggaaa cgcacctgga ccgcggagaa gacaagcgac attgcggtca caaaacaggt
     1801 aagtatgaca acgatgggta ttgcgtacaa gagattgatg gttggcgcta cgtggtagta
     1861 gaccattagc gctgcaagca atagagaagc aatcgcaaaa tcgaataatg ccgcaactac
     1921 atacgtcaat ggcaaaatct cgcgcgggaa ataaaccttc gtgacgagat gtgcatgact
     1981 caccagtcca ctcgttgagc ttgtcaacgc ggtagaaaaa agagtccaaa gcagcaatcc
     2041 cgtaaatgcc attaccggat atggagtacc accagttgga atgcgggcaa aaactgcaaa
     2101 gacaagcgtg taaatcaaca tgagcgagag cggctgcaag attgcccagg caattccgag
     2161 aactgactgc ttgtatctaa ccttcacccg atgtgcacta agcgtgatta acaaatctct
     2221 gtaaagccag agtcctttga agtctcgcag aaatccactg ggagaaaagc tggccggacg
     2281 aatgatcttt acgggacgcc gtctcgccac tgttgaactg ttgtgagctg ttgctatttc
     2341 caccatctaa atatccacta cgaggtcaat tccctgcgct cattcgtttt ctcaaaacca
     2401 gcggtaatca attggggtcg accaatcatt tcaccgtaga gttctgccat ccgtcttcca
     2461 aactgctctc gggtaaagtt cattttgact gcttccctgc cagcgtcgcc gagtcgttct
     2521 cgctgaccct ggtcctccaa caaggagagt atctgacttg ccaactgttc gtcatcgcct
     2581 gcacggtgca gcagtccggt ttcgccatcc ttgattactt caggcacgcc gccaacagcc
     2641 gttgccaata cagctttacc ttgagccatt gcttccagaa tgacgagagg aagaccttcc
     2701 gtttttgtgc taagaacaaa aagatccatt gcggcaagca gtcgatcaac gtcattgcga
     2761 tgatcggtaa aaataaagtt atgcaaaagt cctgccgctg ccagcaacgg ctgaatttca
     2821 ttgaagtggt cacggttgat ttgaacctgt gagtagtcac ctacgataag aaatcgcgct
     2881 tccggaaatt tggaaaccac tcgttttgcg gcccgaatca aagtcgcaaa atccttcgca
     2941 ggagccactc gggctatcat cccaaccatg ctcgctccct gtggtacctc gaattcttct
     3001 cgtacacttt ggtccaaatt acctcttggc tgttttatat cgataccgtc gtaaatcact
     3061 gttccccgcg ctgccggcac cttgaacgcg aagctatgcc aggcgtcatt tgaaacgaaa
     3121 gcaaagtggt taacaacctt gaggaagctt tggtcccgac gtgaaatttc cggataggcg
     3181 cacctcacat gacataaaac tggcaatctg gccaattttc cggcgagtcc ggcgtaataa
     3241 gctgccaaca agtcagagca atgaaccaga tcaacccggc gccgccgaaa ctcgcgagca
     3301 agtcttagcg aagctcgcac ataagcgctc ggatgtttgt agctcggctc gatagcgtca
     3361 aatcgggctg tgtcaaaacg tgctgagctg aacaggtctc gtacgggact gtccgttccc
     3421 tggcaaaagg ccgtatgaac aaagttgtca ttccctaccg cttgagctag tcgtagcgtc
     3481 gcctgttcag ttccaccgat tgtggaccag ggaagaacat gggcgatatg gccacgatta
     3541 gttggcatct attttgaaac tgtcttggat gcgggggacc ataagttctg tctcatccaa
     3601 ttcagcgagc tcaaaaacca tggggcggat tgcaatcccc acacgatcag ctgaagcttc
     3661 ctgctcttat agaagttatt cgcggttctc agtcgctgtg cggcttgttc gtaatcatga
     3721 acgtcgaaaa aacgctttcc ttgagcaaga tctatttgag cggtgcaatt tgctagttga
     3781 ctatccacca atttcaattg ttcgggtgtc aacggcaaag tctgttgagc ctttttcaag
     3841 acctcgatct gactctcgac cattcgtatc tgatcggcgg ccaggctggc atcagagatt
     3901 cgatgacgtg caagaacctg ggtttgataa cgaatccggg cgccttgatg ggccaaacga
     3961 atccaaagat cgaagtcctc acaacggaca aatctctcgt cgaacaaacc tgcagcgatc
     4021 actttttcgc gatttattac actgcaagac gtaatcactg acgactcgta acgcaacaga
     4081 ctttcaaacg tgaccggccc gattgaaggc gaagattgca taaagctctt tccaaccaaa
     4141 gggccctctc caacaagcaa cgcatctgcg taaaccatgt cagtttcggg aacctgattt
     4201 agaaatttca tttgctcggt cagatagttc ggaagccatg tgtcatcgct atctaacagc
     4261 gccagatatt tgcctttagc gatctcgatt gcggcgttgc gagccccgct tggtcctctg
     4321 ttctgctgtt caagataaat tatccggtca gaatagggtg atagaacccg agcgagttcc
     4381 tctgagtcag gtgaaccatc gttcaccacg atcaattcat aatcagaaaa agtttgggcc
     4441 agaactgagc tcacagcgtc ttcaataaat cccgcagcgt tgtaagccgg gataatgacg
     4501 gtcacctgcg gagcacctgg aagtgagctt aatgtcgcta ccgaggctac ctcctcttta
     4561 ctcgccctcg gcacctctgg tccggctggt ccggtctgcc gtatcggctc ccatttcgca
     4621 taacgttcac ctcgtacgaa ttcgtagaag gcgactaccg ccgctaaatt ggcaaggaga
     4681 aaataatgag gcaaggagat cagtccaccg cgtagaccgg tacgctccaa tagataaccg
     4741 accagagcag cagcataacc agccaattgc aaaaccaaaa caaaggcaaa aaacagcgac
     4801 cgggttgcaa ggaccagaga cgatataaaa agcatcgcca gaaagaccgg cacgaggtat
     4861 cgcattacct tgtgcgagaa taattgaaga gcgtagacgc catagcgaaa cgggttgaag
     4921 atatgccgat gtcgccagag atcgttgtat gtttgggtga taacccttac gcgcatgcga
     4981 aactctttgt cggcccgact atttgtctcc tcttcacata tagcgtctgg ttcgtagatt
     5041 gttcgcaggc cctgttccac cattttcgtg gcgataataa aatcgctaca ggcttcatgg
     5101 tagagcggta cgtaagctga acgcctgaca gcgtacaaac agccggaaac gccaatcaat
     5161 gatcgaacgt tactttcgtg tcgcttaagg aacacctcat aattccaata tgaaactgcg
     5221 cctttgccca cgcttgaatt tgacggatct acataaatca attgaccggc cacacagcca
     5281 attgtctcgt cggcaaaatt acgagagatg actcgcagca cgtccggccg gtaaagcgtc
     5341 gtcgcatcag aaaaaagaat gacgtcgccc gtggctagct caacagcgcg attctgtgca
     5401 gcggtcttgc caagacgttc aagctgccgg tgcagacgaa caccctcgtt cgcaaaagat
     5461 ctggcgattt catcggttcg gtctgacgaa cagtcagacg ctacgataat ttcaaacttg
     5521 tctttcggat aatcgagagc aagcgtgttg cgcagcttcg caccaaggtc tcgctcttca
     5581 ttgtaggcag taatgatgaa gctaatagtc ggagtgaaat cttcagactt gacacctctt
     5641 ccccgtaaac ggcttagtaa cacaagtaca attgcataac ctacgtatga atacagcagg
     5701 gcaacaaagc cactccagaa gatcaattga aagataaatt ctacccgcgt aaaactcatg
     5761 attgattctt gtcgctttta aactttatcc ctgggccctg gttaaactat taaggtcgct
     5821 tgatgttttg ctctgatata tgcgaacgag gcaaacacca tatccgacca gataatacgc
     5881 ataccactgg taagccactg ccgcaaaaaa cgcactaacc atgtaaccga tgaggctggc
     5941 ctgtaaaccg attgataagt aatagtaaaa cgaagccctg gattcagtag acgtctccct
     6001 ttcaagcttt ctcagccaca atatcggcgc cgtcagaaac attaagtaaa caatcaaagc
     6061 gccaaatccc atttcagcag agacctgggt gaatgcgtta tgagtcacag tctcatgcaa
     6121 cgacatgatg tgaaagttac ccataccaat tccggttatt ggatttctga gagaaacaat
     6181 aatggaccta ttcagaatct cctgccgcga acccgccgat ccaactggat ccagaccggg
     6241 tatgaaaatt gaaagtaaac gtattccata gcttcccgga gtgagcaaga cgaaaagcgt
     6301 caaaacaatt gggccaacaa tcgcaagcag cagcctgcgc ttacgaccaa tcttccaggc
     6361 cacgacgacg gcaaccacta acaggccaag aaagccacct cgagaataag taacgacgtt
     6421 gccggcaacg atcaatatcg cagttatgat cagaagtatc ttgaggatca atttgcgagt
     6481 cgcaaaggcc atcgtgatcg caatcggtgt catagttaca aaatgaagag caaggtcatt
     6541 tggattgcca aacatccctc cgagcaagcc cttaattcga tagccctcca ctgcaaagtt
     6601 tgccgtttga tagtcacgga tagcgttgaa gctaagaaca aatgtcactg ccagggcaac
     6661 atagatcaat gccattagcc ttcgctcggt acgaacgacg ttgatcatca cgatgaacat
     6721 aaggacggct ttgatgaaga cgtcattaaa ttcattccag gcttccaacg gattaatcgc
     6781 agtcggaatg gaaaacaggc cagccaacgt cagcagtaag accagaccca ccacccgcgg
     6841 ccgagcgctt aagtttccct cgagcaccaa ctgtgagggc agaaagatga taatcgtccc
     6901 aatcgcaagc cagtacgcta ccttgtccat ccacgagagc acagcgaaaa gttcgtacgg
     6961 tcggaaataa agaacgatcg taaagagaaa cagaccggca taactcacca catgcccgcg
     7021 tttgaggatt aggcggacgg ttgattctgg cttcggtagc gactcagttg tgggtggttg
     7081 acttagattg gtgaaaagcg attcgccctg agaggaagct tcaggctcag caccgcgaac
     7141 tggccagggt tcttcgacta acccttcacc cccgcgatta cctggtgtgc gaaatagcgg
     7201 gtcgtagtcc tgaaattcat acctcttcgg cattctaaag actctctggc ggaaacagtc
     7261 taaacaatga ggaagagtag caacaaattg cgcgggtggc actctcagga gggcgctcgg
     7321 cgcggttgac aaacaactcg ctaacaagcg cgatttactt caaaaacccc gaaaatgttg
     7381 atttgggcag aaccgccaga agggatgctc agaccgtgag ttctgaatta gcgattgctg
     7441 gcatacaggc acgcactcgc tcgcgcaacg ccatctcaaa gtgtttcgct tcttcaactt
     7501 ccgtgaagga gagcgtccgc agttcatgct caatgcgcct gcgatagtcc gataccagag
     7561 aatcagatac tcctaaggcg gcagcaactt ccaactgtgt tgaatgatcc gggctcaagt
     7621 agcaatacca gagtacacca agaatgcggt tgtattgctt aaccttgcct cttacggctt
     7681 cgttcaaatt ctttaaaaag agatctactg aacccgcggc ttcacgctct gactcacgct
     7741 tcagcaatcc ttcttccgga ttctctcgtt tatcaacagg ctcgtacgcg ttgctgtttt
     7801 cgttatcgct gtcatcacca tcaagtggca ccaaatagat gtccattaca ggcaggcgag
     7861 acatgacaag acttcgtagc gtgcgcacat cgacaggtga atcgactgca tcgagtacag
     7921 acacaatcaa gtcttcgagg tccgggttag tgatgacaac ttgtgaatca ccagtgcatc
     7981 cgaccattct tgtatcgcgt tgccttacag gtattatctg aacgcgctgt tctacctcgt
     8041 gggctccacg ccttgacttc ttctcgttcc actcactaag tccataaacg cgatcggcga
     8101 gtcgacggtg aggctcgtcc tcactaccgc ttgagtcaaa gcgacgaaaa tttgtacttg
     8161 attgaatgag agttgagatg cggcgcgcaa gccgatagct ttcgggatgt cgtttgcgca
     8221 gctctgcagt tagcaggtta gttaattcaa tctggctgat ctcgcactca atctcatagt
     8281 cggtcatagc cgtttcgaga tagtgttcga aacggttctt gctgagtagc tgtacaaata
     8341 attcctgcgt taaatctgta tacgctgaac cgccaccctc ttcgatgagg aggccagcac
     8401 tgcgcgacgc acgcaccaat gggtgatgcg agacgattcg gtgcagctca gaccagagcc
     8461 gattaacgtc tgacgatcgc agactctcga cccacttcgc gttcttagcg ttgcgttctg
     8521 cactgcggcg aacacgcggt tgctgagggg gcttagcttc agcaccgggc tcgagaactt
     8581 gttcgtaatc ttcactaatg gatttcacag gattgtccga gttgccactt ggttctgggt
     8641 gtgtctctgt tcggtctcaa gaatcggtga gctagtagat cgtctgggct gttttgcgac
     8701 ttgaaacgag gtgctcgttt cctgtctatt ggcaagatcg tgagtacaga gattgttacc
     8761 aggggctggg ccttgggaga ttaccaatct ttcgttatgg aagcgttagg agctgccatc
     8821 gaggcaagcg ctggacaagg ttgtttggcc tgacgatctt ccaccgctat ggaggcggtg
     8881 attcctggat atgtcagccg taaactcttg gctcgactga caaccgcatg gtaacaacat
     8941 tttaaagaat gggcaacatg tttttacgct ctgaaatgag tggaaaaatt gcctgggaca
     9001 gcgggaaata tctggaaata cttgggaatt ggccgttttg tactgttgat cgtagagaag
     9061 taagtactgc cgatgaaggc tagcggccac tcttgtccca ggataatcct cattattgac
     9121 tcggaggctt agcccgtaga cgttgtacgc ggacgtgtga gggtctgaaa aaactatgaa
     9181 aggcttaaag gagagtttgc cttttcagaa atcggtagca tgcccgtcgt cccgcacgct
     9241 cctgtcattt cgaagtgaga aattgtcggc gacggtatcg aacctcgtca gttttcacct
     9301 gggcaattgc gagttttgta acgctgagtt acctcttttg gcccattatc ggagcgcgtt
     9361 tcgaggcgac aacaagactc ccgaaattcc gcgggacctg aaaatcctgg ccgagtcgat
     9421 cctgcaatcc aaaaagcaga actggttcac tccgtgagtt cacaccggtt gaccccgctc
     9481 agctcataac cgcgcgcgcc agcaagaatc ctacccaaac cgtcaacaag ccggctacgt
     9541 tggacacggc aagatttaac aaagcaagaa acagctctcc atctcgaaag agtgtgaacg
     9601 tttgtaaccc aaacgcggaa aaggtggtga agcctcccag gaaccctacc ataatggcaa
     9661 gcctgacggt ttcacttacc aaactacgtt cctgcatcac gtaaaaaagg aatccggcca
     9721 gaaagcaacc gaggacgttg atgatgatcg tccctaccgg aaagctttcg ccgaaacgtc
     9781 gggcaagcaa gacggacatg aaataacgtc cgagcgtacc tgccaggcct gcaaacccaa
     9841 ttaacaggag acgctgcatc taatgttcag gaataaagct cgtcgatgac cgcagcaaag
     9901 cgctcatcga tcactcgacg cctcactttc aaggtaggag tgagttcacc accgtcgatg
     9961 gtgaactcgt ttgga
//
LOCUS       DASSFP010000085         5351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_764141, whole genome shotgun sequence.
ACCESSION   DASSFP010000085 DASSFP010000000
VERSION     DASSFP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764141"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFH91_01785"
     CDS             <1..226
                     /locus_tag="VFH91_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861738.1"
                     /translation="AAFPKHASSPQQLISCADTAMYEAKAAHKNCVRFASGIPSRDSG
                     KPEPTEKNSSSENSSTQVIQSQPANDSTVN"
     gene            501..1547
                     /locus_tag="VFH91_01790"
     CDS             501..1547
                     /locus_tag="VFH91_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6861739.1"
                     /translation="MAFKSIFSLFSSDLAIDLGTANTLVYAKGRGIVVSEPSIVAINK
                     VTNQVEAVGRDAKDMLGRTPGNIVAIRPMKDGVIANFEVTEKMLQHFIRKAHNGKTWV
                     SPRVVIGIPSEITQVERRAVEDSAYRAKASEVYLVEEAMAAAIGAGLPITEPHGNMVV
                     DIGGGTTDIAVISLSGIVYSRAVRVAGNEMDEAIIQYIKRKYNLLIGERTAEAIKIEL
                     GSAYPLDEPLSYEVRGRNLIEGIPKTITITDEEVREALADSISTIINAVRVALERTPP
                     ELSADIVERGIVLTGGGALLKNLDKRLSIETGLPVSLADDPLASVVLGTGKMLSDFDL
                     LKRVKWENTMTSGM"
     gene            1557..2459
                     /gene="mreC"
                     /locus_tag="VFH91_01795"
     CDS             1557..2459
                     /gene="mreC"
                     /locus_tag="VFH91_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET6861740.1"
                     /translation="MASVRTQREIRQRAPIWLVALLFANLAIMAVDARDSDGRQKVVR
                     IWTQTLASPIQSASSKASGATSGFFQRIWNFRSTAEENERLKQQLAETEIELQRAREQ
                     TFENDRLKGLLNLKEQISYDSIPARVIARDASVWFNTIMINRGSSSGIAVNMPVVTAG
                     GIVGRVITVSPFSSQVMLITDEKAGAGAVVGQLSQSGALGSVRGRPENGMIEMRYVPG
                     TEKVEVGDDVLTTGQDGIYPPGLNVGKVADVKHGTATQAHEILIKPGGGLDHLEEVAV
                     LLYRPPQRATPEQSLPNAVEKPKK"
     gene            2562..3104
                     /gene="mreD"
                     /locus_tag="VFH91_01800"
     CDS             2562..3104
                     /gene="mreD"
                     /locus_tag="VFH91_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET6861741.1"
                     /translation="MSQIKIAVVLAVSIVLQLSLRAVWQPLSYIDFPLIVVVYIALQR
                     DAWQALLVGTLAGLIVDASSGGLIGAGGFSKTLTAYIIYFAATRINLENPLIRIPVLA
                     AATVMDSAIYVFWHRVLGSPPSVPFAQTMSYRLIATTVVGTLVLYVIDSILADRSDQR
                     RQFATRRRVARRSTGSLRRR"
     gene            3234..5192
                     /gene="mrdA"
                     /locus_tag="VFH91_01805"
     CDS             3234..5192
                     /gene="mrdA"
                     /locus_tag="VFH91_01805"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6861742.1"
                     /translation="MKYEDTSQNLRARLRFIQALVVVLLAVLGVRLYVLQVVRGEKYA
                     ELAENQRHRRLPIPAPRGVIYDRELHVIVDSRPIYNVILTREDVKGKDLSALVEPLAD
                     GLGLDPDLLRDRFELVKSMPAFESIQVKQGATPGDIAWVESHNLEFPELRVEQQPQRR
                     YPANGSLAHVLGYVGEISPEQLKQQSYKDKGLKPGDIIGQSGIEQIYDDYLRGRDGYR
                     EVVVDSRGRIQDEIETVDPQPGRDLVTTIDSDLQAAAEEQLATSATKRGVIIAMDPNN
                     GEILALASAPTFDPNLFSQRITTKEGRAEYVALLNDPETPLLNRAIQSRYPPGSTWKI
                     PMSVAGLQQGAITIDHSNLVCGGGIQVGNKFTRCMGNHGTPDLKSAITHSCDGYFYRL
                     GLKMGIDGIVSMVDEFDLNKRTGIDLPNEVVSWTPSREFKRRMQPRDPDWRDIDTVYA
                     SFGQVYDIITPISLIRTVAAVSESGKMYTPHLLKEVKPLGDQGTPDYRPAVGFQPLDS
                     SRPNPKNIPIPEDIHHLVVEGMWGVVNNGGTGAAIKMAGFDIAGKTGTAQVVGLGKDT
                     GKNKDHAWFVSYAPAYKPEISMVALIENSGFGGAHAAPAIRAIYDVYYRKTRHEEPPG
                     AVQIAKKPAPSPTPATTTRQGQTIAKNQ"
BASE COUNT         1368 a         1378 c         1398 g         1207 t
ORIGIN      
        1 cgcggctttc ccaaaacatg cttcgtcgcc gcaacagtta atctcctgtg ctgacacggc
       61 gatgtatgaa gcaaaagcgg ctcacaaaaa ctgcgttcgt ttcgcatccg gcattccttc
      121 tcgtgactct gggaagcccg aaccgactga gaagaacagc tcgtcagaaa atagttcaac
      181 tcaagttatt caatcgcagc ccgcgaatga ctccactgtc aactaacctt attaaaaaca
      241 ttcctacgtt tacagtttac ggttgagtgt acacagtgta tcttatgaat ttcggacgtg
      301 ttggtgcgtc ttggggggta atgattgacc cgacggtacc tgaagggacc cacacgcggc
      361 gcttcgttgg agtttaaagg gaagccctgc gaggctggtc gacaggtgat ggccttgctt
      421 ttgggtcagt agttacatca tttgccgccg atcccacccg aagatccatt ctaacaacca
      481 cgggaagtga atcagtctag atggctttca aatctatttt cagtctgttc tcaagtgacc
      541 ttgcaattga tctcgggacg gcaaatacat tggtatatgc aaagggacgc ggcattgtcg
      601 tctctgaacc atcgatcgtc gccattaata aagtgaccaa tcaggtcgag gcggtgggtc
      661 gcgatgccaa agacatgtta ggccgaacac ccggtaacat cgtggcgatc agaccaatga
      721 aggatggcgt cattgcaaac ttcgaagtca ccgagaaaat gttgcaacac tttattcgga
      781 aggcccataa cggaaagacg tgggtcagcc ctcgagtagt catcggtata ccgtccgaaa
      841 taacgcaggt cgaaagacgc gccgtggaag actctgcata ccgggccaag gcctctgaag
      901 tttatctggt tgaggaagca atggccgcag ctatcggggc gggtctgccg atcacagaac
      961 ctcacggaaa catggtggtg gatatcggcg gcgggacaac cgacatcgcc gtgatcagtt
     1021 tgagtggcat cgtctattcg cgcgcagtcc gagtcgcagg gaacgagatg gacgaggcta
     1081 tcattcagta catcaagagg aaatataacc tcctcattgg tgaacgaact gccgaggcga
     1141 tcaaaattga actcggctcg gcttatccgc tcgatgaacc gctttcgtat gaagttcgtg
     1201 gccggaatct catcgaaggt attccaaaga ctattacgat caccgacgaa gaagttcgcg
     1261 aagcactcgc cgactcaatc tcgacaatta tcaacgccgt tcgagttgca cttgaaagaa
     1321 ctccgccgga actttctgca gacattgtgg aacgtggaat tgttctaacc ggcggcggag
     1381 cgttgctcaa gaatctcgac aagcgactgt caatcgagac cggcttaccc gtttcgctgg
     1441 cggatgatcc gctcgcgtca gtcgtattgg gaaccggcaa aatgctttcg gatttcgacc
     1501 tgctgaaacg cgttaaatgg gaaaatacaa tgacaagcgg gatgtaagat cggtagatgg
     1561 ccagcgttcg tactcaacgt gaaattcggc agcgagcccc aatttggctc gtcgcgctgt
     1621 tgtttgcgaa tcttgcaatt atggcggtag acgcccgtga tagtgacgga cgacaaaaag
     1681 tggtccgcat ctggacccag actctcgctt ctccaattca aagcgcatct tccaaggcaa
     1741 gtggcgctac atccggattc ttccaaagga tctggaactt tcgatctact gcagaggaga
     1801 acgaaagact caagcagcaa ctcgcggaaa ccgaaattga actacaaaga gcccgcgagc
     1861 aaacgtttga aaatgatcga cttaaaggcc ttttgaatct gaaagagcag atcagttatg
     1921 actcgattcc ggcgcgggtc attgctcgag acgcctcagt ctggttcaac accataatga
     1981 taaaccgtgg cagtagttcc ggcatcgccg tcaacatgcc tgtggttact gctgggggaa
     2041 tcgtcggtcg agtgataacg gttagcccgt tttcgtctca ggtcatgttg attacggatg
     2101 agaaagccgg cgcaggagcg gtcgttgggc aattgagtca atccggcgcg ctcggctccg
     2161 tgagaggtcg tcctgaaaac ggcatgattg aaatgcgata tgttccgggt acggagaaag
     2221 ttgaagtcgg tgacgacgta ttgacaacag ggcaagatgg gatttatccc ccaggactga
     2281 acgtaggcaa agtcgcagat gtgaaacacg gcaccgcgac tcaagcccac gagattctta
     2341 tcaaaccagg agggggactg gatcatctcg aagaagtggc ggtgttgtta tatcgaccgc
     2401 cccaaagagc tacgccggaa caaagcctgc ccaacgcggt agagaaaccg aagaagtaag
     2461 ataagcacac aaggcgtatg tcgactgata gacgtaaggg ccactccgac cagtcttcac
     2521 gtaactcaag caacattgga ccattcgatt aagtacttag gatgtcgcag ataaagatcg
     2581 ccgtagtcct ggctgtttcc atcgtgctcc aactatcgtt gcgagctgta tggcagcctc
     2641 tgtcctatat cgactttccg ctgattgtag ttgtctacat cgcacttcag cgagacgcgt
     2701 ggcaggcatt attggtcggc actctcgcgg gactcattgt tgacgcttcg agtggaggat
     2761 tgattggcgc cggtggcttc tcaaagactc ttacggctta cataatttac ttcgcagcca
     2821 ctcgtatcaa ccttgagaat cctttgatcc gaatccctgt gctggcggct gcaacggtga
     2881 tggattccgc catttacgtt ttctggcacc gagttcttgg atcgcccccg tcagttccct
     2941 tcgcccagac gatgtcctac agactgatcg ccactacggt ggttggtacc cttgtgctct
     3001 atgtcattga ttcgattctc gccgaccgtt ctgaccaacg aagacagttc gccacccggc
     3061 gccgagtggc tagaaggagt acgggatccc ttcgtcgaag gtgattaccc taaaattctg
     3121 ctttgatctt tagcgtttgc gtgtcaacat tactccggct ccgtctcgaa gttgcgtttg
     3181 caactcctaa agcagattaa aaatctaaag gtcaggcgcc aagtaactgg actatgaaat
     3241 acgaagacac ctctcagaat ctcagagcca gactcagatt tattcaagct ctcgtcgtag
     3301 tgctgctggc ggtactcggg gtcaggcttt acgtcttgca ggtcgtaaga ggcgagaaat
     3361 acgccgaact cgcagaaaat cagcgacatc gccgattgcc gatcccggct ccaagaggtg
     3421 tcatatatga tcgtgaattg cacgtaatcg tcgattcacg cccgatctat aacgtcatcc
     3481 ttacccgcga agacgtcaaa ggcaaagatc tcagcgccct cgtcgaaccg ctcgccgacg
     3541 gcctgggtct cgatcctgac ttgctccggg atcgatttga gctggtgaag tcgatgcccg
     3601 ctttcgaatc gattcaggtc aaacaaggcg ctacgccggg agacatcgca tgggtagaat
     3661 cccacaacct tgagttccct gaactgcgcg tcgagcaaca gcctcagcga cgatatccag
     3721 ctaacggatc actcgctcac gtccttgggt atgtcggcga aattagtccc gagcaactga
     3781 aacagcagtc atacaaggat aaaggactga agccgggcga tatcattggt cagagcggaa
     3841 ttgaacagat ttacgacgat tacctgcgcg gacgtgatgg ttatcgtgag gttgtagtcg
     3901 atagccgcgg ccgaattcag gacgaaattg agacggtcga tccacaaccc ggtcgcgatc
     3961 ttgtgaccac gatcgattct gacttgcaag cagcggctga agagcaactc gcaacctctg
     4021 ctactaaacg aggcgtgatc atcgcgatgg acccgaacaa cggtgagata ctggcgctcg
     4081 cctcggctcc aacctttgat ccgaacttat tctcgcagcg aatcacaaca aaagagggtc
     4141 gtgccgaata cgtcgcgttg ctgaatgacc cagaaacgcc actcctcaac agagccatcc
     4201 aaagtcgata tccgccaggc tccacctgga agattccgat gtcagttgcc ggtctgcaac
     4261 aaggcgcgat cacgattgac cattcgaatc tagtttgcgg tggcggcatc caagtcggaa
     4321 acaagttcac ccgatgcatg ggtaaccacg gtactccaga tctgaagagc gcgattacac
     4381 attcgtgcga tggatacttc tatcgtcttg gtctaaagat ggggatcgac gggattgtgt
     4441 cgatggttga cgagttcgat cttaacaaac gaactggtat tgatcttcca aatgaagttg
     4501 tcagttggac tccctcgcga gagttcaaac gacgtatgca acctcgcgac ccggactggc
     4561 gcgacatcga cacggtctac gcatcgtttg gccaggttta cgacatcatt actccaatct
     4621 cacttatcag gactgtggcg gcggtaagtg aaagcggaaa gatgtacact cctcacttac
     4681 tgaaggaagt taaaccgctc ggagaccagg gtacacccga ctatcgtcca gcagtcggat
     4741 ttcaaccact cgactcctca agaccaaatc cgaaaaacat tccaattccc gaggacatcc
     4801 atcatttggt agtcgaagga atgtggggcg tagtcaataa cggtggtaca ggcgcagcaa
     4861 ttaagatggc cggctttgat atcgccggta agaccggtac ggcccaggtt gtgggactcg
     4921 gaaaagatac cggcaagaac aaagaccatg cgtggttcgt gagttacgct ccggcttaca
     4981 aacccgaaat ttccatggtc gcgctgatcg agaattcggg cttcggtggt gcgcacgccg
     5041 cacccgccat tcgtgccatc tacgacgttt attatcgcaa gacaagacac gaagagccgc
     5101 ccggagcagt tcagattgcg aagaagccag ctccatcacc aactccagca acaaccactc
     5161 gtcaagggca gacgattgca aagaatcagt aacggctcct cccgaccggc attcggcatt
     5221 agataagaca tttcatggtt gcaattattc aaaagagaag ttggcgagat ttcgcctgga
     5281 tactggccct gttggcaatc ggtatagttg ccttcgggac cattcaaatt cgcaacgctc
     5341 agccaactga g
//
LOCUS       DASSFP010000086         3790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_315547, whole genome shotgun sequence.
ACCESSION   DASSFP010000086 DASSFP010000000
VERSION     DASSFP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315547"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1240
                     /locus_tag="VFH91_01810"
     CDS             230..1240
                     /locus_tag="VFH91_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861743.1"
                     /translation="MITGFNTDIEHDGVVYHVQTEDKGLETPIILSLVYSRGEILASK
                     RSRYEDLIAQGFSDEVLSERLKRQHRLICAAIHSGRINDLKKMSGPRVEVEEIVPPPA
                     ILPEITPESLAAEPIESEAIEFEAIEVESIETEAIEAEGEIAEPEITEFLPEPIIVSE
                     PEPPPASDEFVFEIEPTPTQTWLPPEEVVAPEVELEPEVAEGEAEPSIDSPDYIDPYA
                     VYDPRHQSDVSEVVTEGGLVISLLDDDEFHSGEKRTLRVLVSNRRGDNDKPLANASVS
                     IKILGTTFRPLLFTVKTEKDGVATVNAEIPEFTSGRAAVLVRAVARDQAAELRRVIHP
                     NR"
     gene            1253..1471
                     /gene="thiS"
                     /locus_tag="VFH91_01815"
     CDS             1253..1471
                     /gene="thiS"
                     /locus_tag="VFH91_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET6861744.1"
                     /translation="MQVYVNGDSKELTEGASLADLITQLDLPAQRIAVEHNRTVVRRS
                     QWDSTVLGDGDRVEIVHFVGGGTSSNSF"
     gene            1531..2328
                     /locus_tag="VFH91_01820"
     CDS             1531..2328
                     /locus_tag="VFH91_01820"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET6861745.1"
                     /translation="MSSAISDRALNDVLRIGDLSLQSRLIIGTGKYRSHDEMKSAHAA
                     SGAQMVTVAVRRVPLDRSSESFLDHLNPSLQILPNTAGCYTAEDAVRTARLAREALET
                     DLLKLEVIGDQTTLFPDNEQTLEAAKILCKEGFVVLPYFNDDLIMARKLLDAGCAAIM
                     PLAAPIGSGLGIQNPANLQIMREQLPDATIIVDAGVGTASDAAIAMELGADGVLMNTA
                     IAESKSPEQMAKAMKLAIEAGRLAYLAGRMPRRLYASASSPLSGVIK"
     gene            complement(2329..2823)
                     /locus_tag="VFH91_01825"
     CDS             complement(2329..2823)
                     /locus_tag="VFH91_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861746.1"
                     /translation="MKRTLGLFALFLVSHMSVLAQEDKWIRFSPTGSGFEIMIPSEPT
                     EKVETRANYTTHMYSSTHGRTVFLISYSDYTTIPEKALEANRDDFNKQMQATLLSSRN
                     VELDGKTGLEFTSEISAANVKSRIFLIGNRLYQIAALVFKDTQDDKNVDRFFQSFAFL
                     KTPQ"
     gene            complement(2850..>3790)
                     /locus_tag="VFH91_01830"
     CDS             complement(2850..>3790)
                     /locus_tag="VFH91_01830"
                     /inference="COORDINATES: protein motif:HMM:NF020939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6861747.1"
                     /translation="PKPVTQLRINQPDLARRAALERHKLREMIEFCYTEDCFRAYILD
                     YFGDRHHNRECGTCGNCDSGLRLAPAVSKNQTAVQDLDELEPRDLSDDELLRMRKILA
                     CAKRMKGRFGKSVLAGTLRGSAAKNIFQARLNDLSTYGILSGMRHDDIMLYIDSLLFG
                     KCLTSSKGAYPTILITELGERVMREQEIIKLPLPAVAIDKTRPRNNVRSDTPTRLETY
                     NLFKEGLSLPDIAERRSLQLRTIEDHLADCIRVGLEIDLDRLVSFSDQELIATAIERV
                     GYSTLKSLRDVLPGHISYGMIKFVLARYQRTSAQAG"
BASE COUNT          949 a          914 c          974 g          953 t
ORIGIN      
        1 cttcttccta caacagcctt cgagcgagtg ccactcaata actagattga gaatcattgt
       61 acaagctgat gctagtcgaa gtgatcaagg gtgtcaagca acgttcttcg cttgtgatcg
      121 ccaaaaaaaa tgcagattga ccatgaaaaa atccttgtgt taccgtttcg aaaccctcgc
      181 gtgtctgtga ggttttggtt gttaacctga actcgtagat cccacaagag tgattaccgg
      241 attcaacact gatatagagc atgatggcgt ggtctatcat gtccagaccg aggacaaagg
      301 tctggaaact ccgataatcc tgtcgcttgt ctattcgcga ggagagattc tggccagtaa
      361 acgctcgcga tatgaggatc tgattgccca gggattcagt gacgaagttc tgtcggaacg
      421 cctaaaacgt caacatcgcc ttatttgcgc cgcaatacat tccggtcgaa tcaacgacct
      481 gaagaagatg agcgggccgc gggtcgaggt tgaggagatt gtcccgcctc cggccatcct
      541 ccctgagatc acacctgagt ctttggcagc cgagcctatc gaaagtgagg ctatcgagtt
      601 tgaggccatt gaagtcgagt ccattgagac tgaggcaatt gaggctgaag gagagattgc
      661 ggaacccgaa attacagaat ttctgccaga gcccattatt gtttccgaac ccgaaccgcc
      721 gcccgcgtct gatgagtttg ttttcgagat cgaacccaca cccactcaaa cctggttacc
      781 gccggaagag gtcgttgctc ctgaagtcga acttgaaccg gaagtagctg aaggagaggc
      841 agaaccatcc attgattcgc ctgattatat cgatccttat gctgtatatg acccgcgtca
      901 tcagtcggat gtcagcgaag tcgtaacaga aggaggactg gtaatttcgc tgttggatga
      961 cgatgaattt cattcaggcg aaaaacgaac cttgagagta ttagtaagta atcgcagagg
     1021 tgataatgac aagccgctgg caaatgcatc agtttcgata aaaattctcg ggaccacatt
     1081 tcgacctttg ctcttcaccg tgaagacgga aaaagacgga gtggcaacgg tgaacgccga
     1141 aattccagag ttcacttcgg gacgggccgc ggttttggtt cgagctgttg caagagatca
     1201 ggctgcagaa ttgcgccgcg taattcaccc aaacagatga ggcaataagt acttgcaggt
     1261 ctacgtgaac ggcgacagca aagagctcac cgaaggcgct tccttagccg acctaatcac
     1321 tcaactcgat ttgccggctc aaagaattgc ggtagaacac aaccgtaccg tggttcggcg
     1381 ttcacaatgg gactcgacgg ttctaggtga cggtgatcgg gtcgagattg ttcattttgt
     1441 gggtggcggc acttcctcaa acagcttctg agtttcacta caaacgctga aaaggtgtac
     1501 agtgcgttta agcacgaaga aggaatagtt atgtcttcag caattagcga tcgagcacta
     1561 aatgacgttc tgcgaattgg agacctgagt ttgcagtcac gtctcataat cggtactggc
     1621 aagtatcgta gtcacgatga aatgaagagt gcgcacgcgg cttcgggagc gcaaatggtc
     1681 actgttgccg tacggcgtgt gccgctcgac cggagttccg agtcgtttct ggatcacctg
     1741 aacccgtcgc tacagattct gcctaacact gccggttgtt acacggcgga agatgccgtt
     1801 cgaactgcgc gtctggctcg cgaggcactc gaaacagatc tcctgaagct tgaagttatc
     1861 ggggatcaaa ctactctatt tcccgataac gaacagacct tagaggccgc aaagattttg
     1921 tgtaaggaag gattcgtcgt tcttccttac ttcaacgacg atttgattat ggcgaggaaa
     1981 ttgctcgatg caggttgtgc cgcgataatg ccgctggcag ctcccatcgg ctcgggactc
     2041 ggaatacaga atccggctaa tcttcagatc atgcgcgagc aacttcctga cgcaacgatc
     2101 atcgtcgacg ctggcgttgg cactgctagc gatgcggcga tcgcgatgga acttggagca
     2161 gacggagtgc tgatgaacac agcaatagcc gagtcgaagt ccccggagca aatggcaaaa
     2221 gcaatgaagt tggcaatcga agcgggtcgt ttggcttatc ttgccggtcg catgcctcga
     2281 aggctttatg ccagcgcgtc gagtccgcta tccggagtga tcaagtagtt attgcggtgt
     2341 tttaagaaat gcgaatgact ggaaaaagcg atcgacgttt ttatcgtcct gagtgtcttt
     2401 aaagacaagc gccgcaattt ggtacaacct gtttccgatc aaaaagattc ttgacttcac
     2461 attcgcggca ctaatctcac tggtaaattc caatcctgtc ttgccatcga gttctacatt
     2521 ccgactagat aacaaggtgg cctgcatctg tttattgaag tcgtctctat tggcctctaa
     2581 ggctttctca gggatggttg tgtaatcgct gtagctaatg agaaagacag tcctgccatg
     2641 tgtcgaactg tacatgtgag ttgtgtagtt tgctctcgtt tcaacttttt cggtaggctc
     2701 gctgggaatc attatttcga acccgctgcc agttggcgaa aagcggatcc atttgtcctc
     2761 ctgggcaaga acgctcatat gcgaaactaa aaagagcgca aacaatccta gggttctttt
     2821 catttcaaca ctcccattct gaattcacct caacctgctt gcgcacttgt acgctgatag
     2881 cgagccagca cgaacttaat catcccgtag ctgatatggc caggcaagac gtcgcgcaat
     2941 gacttgagcg tcgagtagcc aaccctttca atcgcagttg ctattagttc ctgatctgag
     3001 aacgacacta atcgatcaag atcgatttcc agaccaacac gaatgcagtc cgcaaggtgg
     3061 tcttcaatgg ttcgaagctg caagctgcga cgctcggcaa tatctggcag tgacaagcct
     3121 tccttaaaaa ggttgtaggt ctcaagtctt gtaggcgtat cgcttcgtac attgtttcgg
     3181 gggcgcgttt tgtcaattgc aacagccgga agaggcagtt ttatgatttc ctgttcgcgc
     3241 atcactcgct cgcctaattc cgttatgaga attgtgggat aagcaccctt gctggaggtt
     3301 aggcatttgc cgaagagcag cgaatcgata taaagcatga tgtcgtcatg acgcatcccg
     3361 ctcaaaattc catacgttga aagatcgttg agccttgctt gaaaaatatt tttggcggcc
     3421 gaccctcgaa gtgtcccggc gagcaccgac ttaccaaatc gccccttcat tcgtttcgcg
     3481 cacgcaagga tttttcgcat gcgcagcagt tcatcgtcgc tcaagtcacg tggctcaagt
     3541 tcatcaagat cttgaacagc agtttgattc ttactgacag ctggcgccag tcttaggccg
     3601 gaatcgcagt tcccacacgt gccgcattct cgattgtgat gtctgtcgcc gaaataatcg
     3661 agtatgtaag cacgaaagca atcctcggta taacaaaact cgatcatctc tcggagtttg
     3721 tgtcgctcga gtgcggctct tcgtgcgagg tccggctgat tgatgcgcag ttgagtgaca
     3781 ggtttcgggt
//
LOCUS       DASSFP010000087         2885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_315713, whole genome shotgun sequence.
ACCESSION   DASSFP010000087 DASSFP010000000
VERSION     DASSFP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315713"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFH91_01835"
     CDS             <1..196
                     /locus_tag="VFH91_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861748.1"
                     /translation="AIWRYFRSHKTLSFLKEPIAILGKNHLQGAVASARLLSSRIQLP
                     VRAAAFLNFRLFQAHKKPEL"
     gene            complement(365..1030)
                     /locus_tag="VFH91_01840"
     CDS             complement(365..1030)
                     /locus_tag="VFH91_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6861749.1"
                     /translation="MEHSTTTTIVLADDHHIVRQGLRALLEVENDLHVVGEAGDGLEA
                     VQKVELLGPKVLVLDLMMPGLNGLDVLKQIKKRSPNTQIVILSMYANEAYVLEALSNG
                     ASAYVLKDSKSADLVQAVREAAAGRRYLSPPLTARAIEVYQERAQSTTLDRYETLTAR
                     EREVLHLAAEGRTNSEIAVRLGISSRTAETHRANLMHKLDMHSQAELIRFALRRGIIP
                     MEG"
     gene            complement(1044..2693)
                     /locus_tag="VFH91_01845"
     CDS             complement(1044..2693)
                     /locus_tag="VFH91_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6861750.1"
                     /translation="MSLRGSDTTASDDSDLEGAPTNLLESVNLVEAEDVLRQLAGAFP
                     IANRSQVSAAEFEGKWELAPLPEVDARYQTLVEQIPAVVFLAFLDRGIGEAYVSPQIE
                     SILGFSQDEWLQDPVRWYQQLHPDDKARWSTEAADLFLLGKPLKSLYRVISRDGRTVW
                     FHCEVKMVRNSDGRPWFIHGVAFDISELKTAEEALKKSEEMWRGLFESAPDTVVVIRD
                     SGEIISVNAQVGRMFGYSPNDIIGKSITTLISDPSIVEHLRNRSLGPDASSITSPHEF
                     YGIHQDRSQFPIDIMLNPMVAESSMIICVIRDITMRKSAEESLRNYAQRLKFLSSRLM
                     EVQEVERRNIALELHDEIGQVLTGLKLTLEVSTRLPVEEVGSSINQARDLVNELMARV
                     RNLSLALRPAMLDDLGLLPTLLWHIENYTAQTKVKVNFKHHGLEKRRFTPQVETAAYR
                     LVQEALTNVARHAEVLEATVRLWTDSNKLSIQVEDFGKGFDPDGVTERDSSGLAGMRE
                     RATLLGGQLKIESRSGAGTRLIAELNVADDFAGNLPSTNSY"
     gene            complement(2703..>2885)
                     /locus_tag="VFH91_01850"
     CDS             complement(2703..>2885)
                     /locus_tag="VFH91_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861751.1"
                     /translation="GFPDKFMPFMNQPPGSLLSSHAVMTYGKAIHALRKFTQASLNKE
                     PAWRKPVNRQTSNDPS"
BASE COUNT          712 a          750 c          695 g          728 t
ORIGIN      
        1 ggccatatgg cgctatttcc gcagccataa aacactctcc tttctaaaag agccaattgc
       61 gattctgggc aagaatcatc ttcaaggagc tgtcgctagt gcgcgtttac tgtcgagtcg
      121 cattcaactc cctgtccgag cggcggcgtt ccttaacttt cgactgtttc aagcgcacaa
      181 aaaacctgaa ctttgaaacc cgggcgagtt tttgagcaca agtcaaagca gaactccgac
      241 caaattgtag ggaacaaggg gattattagc ctggaacgag tattagtcta ttcgtagacc
      301 tacgtagatg ggctacgaaa gctccgtatt tttatggaga atgagtgtct gaacgatggc
      361 gcgctcagcc ttccatcgga atgatgcctc tgcggagcgc aaagcggatc agttctgcct
      421 gtgaatgcat atctaatttg tgcattaagt tggcacggtg agtctcagca gtacgtgaac
      481 ttattcccag gcggacagcg atttcactat ttgtccgacc ttcagcggcg agatgtagca
      541 cttccctttc gcgcgccgtc agagtttcat atcgatccaa agtggttgac tgcgctcgtt
      601 cctgatagac ctcgatcgcg cgcgcggtaa gtggaggact aaggtatcgc cgaccggctg
      661 ccgcttcacg aactgcctgt acaagatccg cagactttga atccttcaat acataagctg
      721 acgcgccgtt gctgagcgct tcgagtacat aagcttcatt agcgtacatc gagagaatga
      781 cgatttgcgt atttggagac cgtttcttga tctgtttcaa cacgtccaga ccattgagtc
      841 cgggcatcat caagtccaac actaaaactt ttggaccgag aagctcaact ttctgcactg
      901 cctccaaccc atcgcccgcc tcaccaacca catgtaaatc attctcgacc tcaagcaatg
      961 ctcgtaaacc ttgccgcacg atgtgatgat cgtcggcaag gacaatggta gtcgtggtcg
     1021 aatgctccat tcagaggtct ccgtcaataa gaattggtgc tgggaagatt accggcaaag
     1081 tcatctgcga cgttcaattc agcaatcaag cgagttccag caccggatct cgattcgatt
     1141 ttgagttgac ctccaagcag cgtagctcgt tcgcgcatgc cggcaaggcc gctcgaatct
     1201 cgctccgtga ctccgtccgg gtcaaagccc ttgccaaaat cttcaacttg tattgatagc
     1261 ttattcgaat cagtccacag tcgcacagtc gcctccagga cttctgcgtg acgggcgacg
     1321 ttggtgagcg cttcctgaac gaggcggtat gctgctgttt caacttgtgg cgtgaacctc
     1381 ctcttctcaa ggccgtgatg cttgaaattc accttcactt ttgtttgagc cgtgtaattt
     1441 tcgatgtgcc agagaagagt gggcaataat ccaagatcgt ccaacatcgc tggtcgcaaa
     1501 gcgagcgaca agtttctaac gcgcgccatc aactcgttca ccagatctcg cgcttgattt
     1561 atgctggagc cgacttcctc gacaggtaac ctggtactca cttcgagcgt caacttaagt
     1621 cctgtcagta cctgaccaat ttcgtcgtgc aactccagag caatattgcg tcgttcaact
     1681 tcctgaactt ccatgagtcg gcttgagagg aatttcaagc gttgagcgta gtttctcagc
     1741 gactcttcgg cagacttccg catcgtgata tcgcgaataa cgcagatgat catactcgac
     1801 tcggcgacca tcggattgag catgatgtca attggaaact gtgagcgatc ctggtgaatt
     1861 ccatagaact catgcggaga tgtaatagat gaagcgtccg gtccaagtga tctgttgcgc
     1921 aggtgctcga cgatcgacgg atcggaaatg agtgttgtaa tcgacttacc aattatgtcg
     1981 ttcggcgaat aaccaaacat cctaccgact tgagcattaa cactgatgat ttcacctgaa
     2041 tcccgaatta caaccacagt atcaggcgct gattcgaaca gaccgcgcca catctcttca
     2101 cttttcttaa gcgcctcttc cgctgtctta agttctgaaa tgtcgaaggc aacaccgtga
     2161 atgaaccagg ggcgaccatc gctgttgcgc accatcttta cttcacaatg aaaccaaaca
     2221 gtgcgcccgt cacgtgagat gacccggtaa agcgatttga gaggcttgcc cagcagaaat
     2281 agatctgcgg cttcagtgct ccaccgagct ttatcatccg gatgaagttg ttggtaccaa
     2341 cgaaccggat cctggagcca ctcatcctgt gaaaagccca ggatcgattc tatttgagga
     2401 ctgacgtagg cttcaccaat tccacggtca agaaacgcga gaaatacaac cgctggaatt
     2461 tgttcgacca gcgtttgata gcgagcatcg acttccggta agggcgcaag ttcccatttt
     2521 ccttcaaact cagcggctga aacttgtgaa cgattcgcga ttggaaaagc accggcgagc
     2581 tgtcgcagaa catcttccgc ttcgaccaga ttaacgcttt cgagaagatt ggtaggtgca
     2641 ccttcaaggt cggagtcgtc ggaagctgtg gtgtcagaac cgcgtaaact catatttctt
     2701 gattaggaag ggtcgttgct cgtttgtcta tttaccggtt tacgccacgc cggctcctta
     2761 ttaagagaag cctgcgtaaa ctttcttaat gcatgaatcg ccttgccgta agtcataacc
     2821 gcgtgtgacg acagtaacga acccggaggc tgattcataa acggcatgaa tttgtctgga
     2881 aaccc
//
LOCUS       DASSFP010000088         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1505926, whole genome shotgun sequence.
ACCESSION   DASSFP010000088 DASSFP010000000
VERSION     DASSFP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505926"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /gene="araD"
                     /locus_tag="VFH91_01855"
     CDS             complement(<1..1598)
                     /gene="araD"
                     /locus_tag="VFH91_01855"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HET6861752.1"
                     /translation="MKLRSQRWFGENDMRSFGHRSRTKQMGYSLEDFQGKPVIAIINT
                     WSDLATCHSHFRTRAEEVKRGVWQAGGFPVELPAMSVTETFMKPSPMMYRNFLAMETE
                     ELLRSQPIDGVVLMGGCDKTTPGLLMGAISMNLPSIYLPAGPMLRGNWNGQVLGSGSD
                     VWKYWAEKRAGNISDCEWSEMEDGIARSPGHCMTMGTASTMTAIAETLGLTLPGASSI
                     PAVDANHSRMAANCGRRIVQMVFDDLKPRDILSNASFTNAITADMAIGGSTNAIIHLL
                     ALAGRACVPLDIREFDRISARTPMIVNLRPSGEFLMEDFYYAGGLRAVLKELTDLLDL
                     DAATVSGQTLGQNIQTATITNQQVIRPTTNPVARSGGTVVLFGNLAPDGAVIKPTAAE
                     PRLLKHTGRAVVFRDYNDLSARIDSESLEVDQDSVLVLQNAGPLGGPGMPEWGMLPIP
                     QKLLKQGVRDMVRISDARMSGTAYGACVLHVAPESFIGGPLALVQDGDMIELDVESRK
                     LQMRVPDEELNERRSRWKPRPPHY"
     gene            complement(1595..2587)
                     /locus_tag="VFH91_01860"
     CDS             complement(1595..2587)
                     /locus_tag="VFH91_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6861753.1"
                     /translation="MIRWGLIGCGDIARKRVAPALNNSANSTLVAVSRANRDLLDTFA
                     DEFGAEKRYSDWRDLVLDREIDAVYVATPVHLHAQQTIAAADTGKHVLCEKPMALNVA
                     QCDQMISACSDNGVKLGIAYYRHFYPVIRRLREIVESGEIGVPVVVQINAFEWFNPEP
                     GHPRKWLLQPELSGGGPMFDFGCHRVEVLLDVFGAIKDVKSTMGNVLFDREVEDVATA
                     QFQFENGAYANLSVTHAAFEPQDTLHIFCSRGSIHVPALNSGRMHILTETGERVEDHF
                     GANNIHQPVIEDFVSAILNDRAPVVTGEIGRRVAEVIERIYGNTEPLTVNVSKR"
     gene            complement(2762..3664)
                     /locus_tag="VFH91_01865"
     CDS             complement(2762..3664)
                     /locus_tag="VFH91_01865"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF002958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HET6861754.1"
                     /translation="MHPSELRHTLSGVIAFPITPFKQDLSLDLPGLHHNLTKLLEHPV
                     SAVVVAGGTGELYSLTPSEYARVIELAVHAIESRIPVIAGVGFGQRLGVEMAQAAERA
                     GADGILAFPPYYPNADDEGLFEYYRLIGAATELGMLIYSRDWTRFTPSMVERLTTIPT
                     LIAWKDGQGDIRMLQSIMNRIGERLHWIGGAGDDMVAAYYSTGIRTFTSSIATVAPRL
                     SLRLHELGDAGDMDDLAELLARCVVPLYSIRARRKGYEVSTMKAMMDMVGLNGGPVRP
                     PLVNVTAEEEDELRMILGGWEKFL"
     gene            complement(3684..>4006)
                     /locus_tag="VFH91_01870"
     CDS             complement(3684..>4006)
                     /locus_tag="VFH91_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861755.1"
                     /translation="ERPAVTHMRDWQLPGFTTGSGCVIPGLSQKLYEACSDGDFESAL
                     KIRSNFIGLEDLRDSWGPARVLHHAVELAGIAEVGPIPPYVSPLSDEQITELQQVVDE
                     VAKR"
BASE COUNT         1002 a         1134 c          932 g          938 t
ORIGIN      
        1 tgataatggg gtggtcgggg tttccatcgc gatcttcgtt cattcaactc ctcatcggga
       61 acgcgcatct ggagcttgcg cgactcaaca tccaactcga tcatgtcgcc atcttgaacc
      121 agcgctaatg gtcctccgat aaacgactcg ggcgcaacgt gtaatacgca tgcaccatac
      181 gccgttccac tcatacgcgc atcagagata cgaaccatat cgcgtacgcc ttgcttgagg
      241 agtttttgcg ggatcggcag cattccccat tccggcatgc ctggtccacc taatggcccg
      301 gcattctgta acaccagcac agaatcctga tcgacttcaa gcgactccga atcaatacga
      361 gctgaaagat cattgtaatc gcggaaaaca acggccctgc ctgtgtgctt caacaaacgc
      421 ggctcagcag cagtcggttt gatgactgcg ccgtctggcg cgagattacc gaacagaacg
      481 actgtgcccc cagatctcgc aaccggattt gtcgtggggc gaattacttg ctggttggtt
      541 attgttgccg tctgaatatt ttgtcccaac gtctgacctg aaacagtggc cgcatccagg
      601 tcaagcaagt cagttaactc tttcaggact gctcgcaatc caccggcgta ataaaagtct
      661 tccatcaaaa actcgcctga cggacgcaga ttgacaatca ttggtgtgcg agccgagatc
      721 cgatcaaact cgcgaatatc gagtgggaca caggcacgac ctgcaagtgc tagtagatgg
      781 atgatggcat tagttgaacc gccgatagcc atgtcggcgg tgatggcgtt tgtaaaggac
      841 gcatttgaga gaatgtcacg cggtttcaaa tcgtcgaaaa ccatctgcac tattcgcctg
      901 ccacaattcg cggccattcg agaatggttt gcgtcgactg ctggaatgga agatgctccg
      961 ggtagagtta aaccaagcgt ctccgcaatc gcagtcatag tcgaagcggt tcccatcgtc
     1021 atacagtgac caggactgcg agcgataccg tcttccatct cggaccattc acagtcagaa
     1081 atatttcccg cacgtttctc agcccagtat ttccaaacat cacttccact cccgaggact
     1141 tgtccattcc agttgccgcg taacattgga ccagcaggca ggtagatgga aggcaaattc
     1201 atgctgatcg cacccatcaa caatcccgga gtcgttttgt cgcaacctcc catcaagact
     1261 acgccgtcga ttggttggct gcgcagtagt tcttcagtct ccattgccag aaagttccgg
     1321 tacatcatcg gacttggctt catgaaagtc tcagtaaccg acatcgcagg aagctcaact
     1381 ggaaagccgc cagcctgcca gacgcctcgc tttacttctt cggcgcgagt tcgaaaatga
     1441 ctgtggcaag tagcgagatc actccacgta ttgatgatcg caatgactgg tttaccctgg
     1501 aaatcctcca ggctgtatcc catctgtttt gtacgcgaac gatgtccaaa ggaccgcatg
     1561 tcgttctcgc caaaccatct ttgacttcta agtttcatct tttggacacg ttaactgtca
     1621 gcggctctgt gtttccataa atcctctcga tcacttcggc gacccttcga ccaatttcac
     1681 cggtcacaac tggagcacgg tcattcagga ttgcactaac aaaatcttct atcactggtt
     1741 gatggatatt attggctccg aaatgatctt ctacccgctc accggtttct gtaagaatgt
     1801 gcatcctgcc gctattaagt gcaggtacat gtattgatcc gcgggaacag aagatatgca
     1861 gagtgtcctg cggctcaaac gccgcatgag tgacactcaa atttgcgtag gctccattct
     1921 caaactgaaa ctgcgcagtg gctacatctt caacttcacg gtcaaacaag acgttgccca
     1981 tcgtactctt tacgtctttg atcgccccaa aaacgtcgag taagacttca actcgatgac
     2041 atccgaaatc gaacattgga ccaccaccgg agagctctgg ctgcagcaac cacttgcgag
     2101 gatgaccagg ctcaggattg aaccactcga atgcatttat ctgaacaacg acgggtactc
     2161 caatctcgcc cgactcgact atttctctta agcgacgtat gaccggatag aagtgacgat
     2221 aataggcgat gcccagctta actccgttgt cgctacacgc cgagatcatt tgatcgcatt
     2281 gtgcaacatt caaagccatt ggtttctcgc aaagaacatg ctttccggtg tcagcggcag
     2341 cgattgtctg ttgtgcgtgc aaatgaacag gtgtagcaac gtaaacagcg tcgatttccc
     2401 tgtccagaac aaggtctcgc caatcgctgt atctcttttc tgcgccaaac tcgtcagcaa
     2461 aagtatcaag cagatcgcga ttggctcgac taaccgcaac cagtgtggaa ttcgcagagt
     2521 tgttcagcgc cggagcgaca cgtttccgcg caatgtcacc acatccaatc aatccccaac
     2581 gtatcatctt tttggagtgc gccggcccgg cggcgctttg ttattctgaa gactcgctaa
     2641 tacgaagtgc aaaaaaagca cccacccgcc taagcggtac tcttaatcga gtgacatttt
     2701 taaataagga cccgtttaat cgagatacca aaaagcggcg ccgggccgcc ccactccaaa
     2761 atcacaagaa cttctcccag ccgccaagaa tcattcgtag ctcgtcttcc tcttccgctg
     2821 taacgttcac taacgggggt ctcactggcc caccattcaa cccaaccata tccatcattg
     2881 ctttcatcgt cgacacctca taccccttgc gtcgagcacg aatcgaatac agtgggacga
     2941 cacacctcgc gagaagctca gccagatcat ccatatcgcc cgcgtcgcca agttcatgga
     3001 gacgtaacga caatcgggga gcaacggtcg cgatgctcga ggtgaatgtg cgaatgccag
     3061 tgctgtagta agcagcaacc atgtcgtcgc cggccccacc gatccagtgt aaccgctcac
     3121 cgatacgatt catgatcgac tgcaacatcc gtatgtcgcc ctgaccatcc ttccaggcga
     3181 taagagttgg aatggtcgtg agtcgctcaa ccatgctcgg agtaaacctg gtccaatcgc
     3241 gactgtagat caacatgccc aattcagtgg cggcacctat caaacggtaa tactcaaaca
     3301 atccttcgtc atctgcattt gggtaataag gcggaaaggc gagaataccg tctgcgccag
     3361 ctctttcagc agcctgggcc atctcaaccc caagccgttg gccaaagccc accccggcga
     3421 tcaccggaat gcgactttcg attgcgtgga cagccagctc aatgactcgt gcatattccg
     3481 agggcgtcag cgaatacaat tcgcccgtgc caccagcgac gaccacggct gaaaccgggt
     3541 gttcgagcaa tttggtcagg ttgtgatgaa gtccgggaag atcgagtgat agatcttgtt
     3601 taaagggcgt gattggaaac gcaatcacgc cagaaagagt gtgcctgagt tccgatggat
     3661 gcattttgta ttgcgccgac ctatcagcgc tttgctactt catcaacgac ctgttgaagt
     3721 tctgttatct gctcatccga caatggcgac acatacggcg gaattggacc aacctcggca
     3781 atccccgcca gctcgaccgc gtgatgcaac actcgcgctg gtccccaact atctcgcaga
     3841 tcttcaagcc ctataaagtt ggagcgtatc tttaaagccg actcaaagtc accatcacta
     3901 catgcttcat acaatttttg acttaatcct ggaatgacac aaccggaacc agtcgtaaaa
     3961 cccggcagtt gccaatcgcg catatgcgtg actgcgggcc tttcac
//
LOCUS       DASSFP010000089         5083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1806505, whole genome shotgun sequence.
ACCESSION   DASSFP010000089 DASSFP010000000
VERSION     DASSFP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806505"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFH91_01875"
     CDS             complement(<1..957)
                     /locus_tag="VFH91_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET6861756.1"
                     /translation="MRLRTNLLYLVVGTIVPLIALATVLGFLLVDRERDAVRQGAINR
                     NRAFMTAVDAALKGHISTLQALATVSSLEREDLETFHNDAVRVLDAQTDWQSIILTRP
                     NSEQLIVASRIGPETTAQTVDEPSFRRVVEEEQPVVGELSYRENSKRYGIAVRVPVRQ
                     RGRLAFVLSAVVNPEQFSRLIMDQHLPNGWISGLVDPTGHLIARVPARPNSDVATDAF
                     MNALKTGPEGWYRGRTLEGADTFTAFKTSEFSKWSVGMAIPIQEVNAGVVRAVWVMLA
                     GTLITISLALGFAYWMGRRITTPIDALADAARAMGRDSQTAPI"
     gene            complement(1072..2073)
                     /locus_tag="VFH91_01880"
     CDS             complement(1072..2073)
                     /locus_tag="VFH91_01880"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HET6861757.1"
                     /translation="MHPITLIPGDGIGPEVASNVVRIIEASGVDIQWETHFAGAQALE
                     KFGETLPKELLDSIIRNKVALKGPITTPVGKGFTSVNVGLRKTLDLYANLRPVKALPN
                     VECRYPELDLVVVRENTESLYAGLEHVVVPGVVESLKIITEKASTRIAKFAFEYSRKE
                     SRKKVTAVHKANIMKLSDGLFLDCFRKVSESYPEIAAEDKIVDNACMQLVMRPEQFDV
                     MLLENLYGDIVSDLCAGLVGGLGLVPGANIGEKGAVFEAVHGSAPDIAGQGIANPTAL
                     LQSGILMLRYLNEREAADRVENALMAVFREGKVRTRDIGGTAKTAEFADAIIQKINA"
     gene            2193..3323
                     /gene="thiO"
                     /locus_tag="VFH91_01885"
     CDS             2193..3323
                     /gene="thiO"
                     /locus_tag="VFH91_01885"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HET6861758.1"
                     /translation="MKDNLTNFDVIIVGGGVIGLSIARSLAFRSVRNICLIERGECGT
                     EASHAAAGMLAPQAEADAADQFFQILCRSRDQYPDWAVSLKEETGIDIELDLTGTLYL
                     SFSEVEQRELDHRFAWQSKAGLPVERLSTADVRTLEPQISSLIFGALFFPRDVQVENR
                     RLLAALANSVHGLGIPVFNETNVEEIIVRQGKVEGVKTNKGLLGSSTVIVAAGAWSDL
                     NGALKFPVEPVRGQMVCMSAKPELTRHVIYSPRGYLVPRQDGRLIAGSTTEHVGFLKT
                     VTAGGVKSILVNAAEISAAVEKLPILSSWAGLRPRAPDGLPLLGPCDEIGGLIYATGH
                     YRNGILLAPLTGELMAETVVNGRIPSEVASFSPNRFKFAGVI"
     gene            3330..4694
                     /locus_tag="VFH91_01890"
     CDS             3330..4694
                     /locus_tag="VFH91_01890"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6861759.1"
                     /translation="MNPTKPKLSGLPLALILLLSWAGPHLAQTPMVKSSNPDERTAQA
                     LFEDANGYLGRKYQDFNKQKLPYDAKLEQRTKQEQLSLAKQNAATLQARKKFKDDDLY
                     YLGLLYHLANDGGAALTSMHQFLKDHADGSKAQSARTVVVLYATKANDYAVAEAAVKD
                     YQVHQPQVAEDRYNMEFLLADAYQRSNEYPKVVEHAEKIVEAARTFASANKTETFKRD
                     DMLLKSALMLSNGYLKTEQKQKAIQMFSDLRKLSIALPSGSLYKQTTIRLKALDPQFD
                     TQQLFDDVSALPKTRLPDIVATQWIDQQPVKFSDLRGQVVLLDFWASWCGPCRYTFPK
                     LVEWNKTYKDKGLVILGVTKYFGRADTRPLTPGEELVYLREFKKRNQLSYGFIVGDSN
                     VNDLNYGVLSIPTTFLIDRQGTVRFISTGSGEEEIAQLGAMLKKVMAEPVNPMSPSES
                     SDKK"
     gene            4704..>5083
                     /locus_tag="VFH91_01895"
     CDS             4704..>5083
                     /locus_tag="VFH91_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET6861760.1"
                     /translation="MEGQGIDLVRILIAFIAGIASFLSPCVLPLVPGYISLISGVSVD
                     GLKEGTSNRRAVILNSLAFNAGLSVIFLILGTTAGLVGAAITSNPLVRIIGGLVIVAF
                     GLQLIGLLKISALYKDTRMFSKDKP"
BASE COUNT         1239 a         1280 c         1306 g         1258 t
ORIGIN      
        1 gattggcgca gtttgagaat ctcgtcccat cgcgcgagca gcgtctgcca acgcgtcgat
       61 tggagttgta atgcgccggc ccatccagta cgcgaatccc agcgcgagag agatcgtgat
      121 caaagttcct gccaacatca cccagactgc acgcactacc ccggcgttca cctcttgaat
      181 gggaatcgcc attcccacac tccacttact aaactcagag gttttgaaag cggtaaaagt
      241 gtcggctccc tcaagggtac gtcctctgta ccaaccttct ggtccggtct tgagagcatt
      301 cataaaggca tcagtcgcca cgtccgaatt gggccgtgcc ggcaccctcg caattaaatg
      361 gcccgtggga tccacgaggc cactaatcca tccgttaggc agatgctggt ccatgattag
      421 ccgtgaaaat tgctcaggat tgacaacggc cgaaagcaca aaggccagtc gaccacgctg
      481 gcgcaccgga accctgacgg cgattccgta tctcttcgaa ttctctctgt aactcaactc
      541 gccaacaaca ggttgttctt cttcgactac gcgtcgaaaa ctaggctcgt ccacggtttg
      601 cgccgtcgtt tcgggtccga tcctactcgc gacaatcagc tgttcactat ttggtctggt
      661 gagaatgatg ctctgccagt cagtttgcgc atcgagcact ctcaccgcgt cgttatgaaa
      721 tgtttccagg tcttctcttt ccagactgga aaccgttgca agcgcttgca gagtcgatat
      781 gtgacctttt agcgcagcat cgacagctgt catgaacgcg cggttacggt ttatcgcgcc
      841 ctgtcgaact gcgtcgcgtt cccgatcaac cagcagaaag cccagcactg ttgcgagcgc
      901 aattagcggt acgattgtgc caaccacgag atagagtaga ttggtgcgca gtctcatgga
      961 gtcggaaaaa tgttaattag gtagaccttt tttctaactc gtctgggcga aacgagtgaa
     1021 gggggtaaat tgttaagacg cagaattcgt aagaggaaat caatgggaga attacgcgtt
     1081 aatcttttgg ataatcgcgt ctgcaaactc agccgttttt gcagtacctc ctatatctcg
     1141 agtgcgtact ttgccctctc taaatacggc catcaaggca ttctcaactc gatctgcggc
     1201 ttcccgttca ttcaggtagc gcagcataag aattcccgat tggagcagtg cggttggatt
     1261 ggcaatgccc tgacccgcga tgtcgggtgc cgagccatga actgcttcaa acaccgcacc
     1321 tttctctccg atgtttgcac ccggaacaag tccaagaccg ccaactaaac cggcgcaaag
     1381 atctgaaacg atatctccat aaaggttttc caacaacatc acgtcgaatt gctctggccg
     1441 cattaccagt tgcatgcaag cgttatcgac gatcttgtct tctgctgcaa tctccgggta
     1501 gctctcgctt actttacgaa agcaatcgag gaacagtccg tcggaaagct tcatgatgtt
     1561 agctttgtga acggcagtga ccttttttct cgactctttg cgcgagtatt cgaatgcaaa
     1621 tttagcgata cgagttgagg ctttttctgt gatgatctta agactctcaa caacacccgg
     1681 cactaccaca tgttctaaac cggcgtacag actctctgta ttttcgcgga caacgaccag
     1741 gtcgagttcc ggataccggc actctacatt tggtagggcc ttcaccggtc ggagattcgc
     1801 gtacaaatcc agggtctttc ggaggccaac gttaactgag gtgaagccct taccgactgg
     1861 cgtcgtaatt ggccctttca acgccacctt gttccttatg attgaatcaa gcagttcttt
     1921 aggaagagtt tcgccaaatt tctcaagcgc ctgagcgcct gcaaaatggg tttcccattg
     1981 tatgtcgaca cccgaagctt caataattcg tacaacgttt gaggctactt cggggccgat
     2041 gccgtcgcct ggaatcagtg tgataggatg catagtctcg tgaatatagc agtgcgaacg
     2101 cgtttagaac atcgaggtta gcataaagcc tttggtcagt tgctgacaat ccagtccgtt
     2161 ctcgcctgcg ttatcatcag taacaatttc cggtgaagga taatctaaca aacttcgacg
     2221 ttatcatcgt tggcggcggt gtcatcggac tgtcgatcgc acgctccctg gcgttcagaa
     2281 gtgtccggaa tatttgtctg atcgaacgtg gggaatgtgg aacggaagct tcgcacgctg
     2341 ccgctggaat gttggcgccc caggcagaag cagatgccgc cgatcaattt tttcagatac
     2401 tttgccgcag tcgagaccaa tatcctgact gggcggtgtc acttaaagaa gagaccggga
     2461 tagacatcga acttgacctg acagggacgc tctatctttc cttctcagaa gttgaacaac
     2521 gggaactgga tcaccggttt gcatggcagt caaaggcggg tcttccggtc gagcgacttt
     2581 caaccgctga cgtacgcacc cttgaaccac agatctcatc attgatattt ggagcgttgt
     2641 tttttccgcg agatgttcag gtcgaaaaca ggcgactttt ggcagctctg gcaaacagtg
     2701 ttcacggact gggtatcccg gtattcaacg agacgaatgt cgaagagata atagttcgac
     2761 agggcaaagt cgaaggagta aagacgaaca aaggtttgct gggtagttcg accgttatcg
     2821 ttgcggcagg agcctggtcc gaccttaacg gcgccttgaa gtttccagtc gaaccagtgc
     2881 gaggacagat ggtatgtatg tcggctaagc ctgagctaac ccggcatgtc atttatagtc
     2941 cgcgcggtta cctggttccg cgtcaggacg gtcgcttgat tgcgggttcc acgacagagc
     3001 atgtgggctt tctgaagact gtcactgcag gtggcgtcaa atccattctg gtgaatgctg
     3061 ccgagatttc cgccgcggtt gaaaagttgc caatattaag ttcgtgggct ggcttgcggc
     3121 cacgtgcccc cgacggtttg ccactgctgg gtccttgtga tgaaattggg gggcttatct
     3181 atgcaaccgg gcactacaga aacggaatcc tactcgctcc cctgactgga gagctcatgg
     3241 ccgaaacggt tgttaacggt cgtattccaa gcgaagtggc ctctttcagt ccaaatcgct
     3301 ttaagtttgc aggcgtgatc taagtcgtaa tgaatcccac taaaccgaaa ctgtcgggtt
     3361 tacccctcgc tttaattttg ttgttgagtt gggccgggcc gcatttggcg cagacgccga
     3421 tggttaagtc atcgaatcct gacgagcgca cagcccaggc tctattcgaa gacgcgaatg
     3481 gctatctcgg gcgcaaatat caagacttca ataaacaaaa gcttccctac gacgccaaat
     3541 tagagcaacg caccaaacaa gagcagctga gtctggctaa acagaatgcc gcgacacttc
     3601 aagcgcggaa gaagtttaag gacgacgacc tgtattacct gggattgctt taccacctcg
     3661 ccaacgacgg aggtgctgcg ctcacttcta tgcaccagtt tctgaaagat catgccgatg
     3721 gctcgaaggc tcagagtgcc cgaactgtgg tagtgctgta cgcgacgaaa gctaacgatt
     3781 atgcagtcgc tgaagcagcc gtcaaagact atcaggtcca tcaaccgcaa gtggctgagg
     3841 accgctataa catggagttt ttgttggccg acgcgtatca acgttcaaat gagtacccca
     3901 aagtagttga gcatgctgag aagatagtag aagccgccag gacatttgcc agtgcaaaca
     3961 agacggagac cttcaaacgg gacgacatgc tgctcaaatc tgccctcatg ctgtccaatg
     4021 gatatttaaa gaccgaacag aaacagaaag cgatacagat gttttcggac ttacgaaaac
     4081 tgtcaatcgc gttgccttca ggcagtctgt acaagcagac aacaattcgt ttgaaagctt
     4141 tggatccgca gtttgacaca caacagttat tcgacgacgt ctcggcgctg ccaaaaacca
     4201 ggctcccgga tatcgttgcc actcagtgga tcgatcaaca accggtgaaa ttttctgatc
     4261 tccgcggtca ggttgtctta cttgatttct gggcgagctg gtgtggtcca tgtcgttata
     4321 cgtttccgaa actggttgag tggaacaaga cctataaaga taaaggactg gtgatactcg
     4381 gcgtaacgaa gtacttcggc cgtgcggata ctcggccgct cactccgggc gaagagttgg
     4441 tctatttgcg cgagttcaaa aaacgtaatc aactctcgta cgggtttata gtcggtgact
     4501 cgaatgtcaa cgatctaaat tacggtgtgc tctcaattcc caccacgttc ctaattgatc
     4561 gccagggtac ggtgagattt atttcaacgg gttccggaga ggaagagatc gcccaacttg
     4621 gtgcgatgct aaaaaaggtg atggcagaac cggtgaatcc gatgagtccg agcgaaagtt
     4681 cggacaaaaa gtaggaggca gaaatggaag ggcagggaat agatttagta cgaattctga
     4741 ttgcttttat tgccggcatt gcgtcatttc tttctccatg tgtgctgccg ttagtccctg
     4801 gttatatctc gttaatttcc ggagtaagtg ttgatggcct gaaggaagga acaagtaacc
     4861 ggcgggctgt gattctcaat tcgctcgcct tcaacgccgg tctatctgtc atctttctga
     4921 tacttggaac gacggcgggc ttagtcggag cagcgattac cagcaacccc ctggtaagaa
     4981 tcattggtgg cctggtcatc gtcgcctttg gcttgcagct aattggacta ctgaagattt
     5041 ctgcgttgta caaagacacg cggatgttct cgaaggacaa acc
//
LOCUS       DASSFP010000090         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1506193, whole genome shotgun sequence.
ACCESSION   DASSFP010000090 DASSFP010000000
VERSION     DASSFP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506193"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..1190
                     /locus_tag="VFH91_01900"
     CDS             480..1190
                     /locus_tag="VFH91_01900"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HET6861761.1"
                     /translation="MSKFFADAFRHLGGNRGVPAIEVRFYPYAGLRHTIRIRSNRVYV
                     RLSDITKDAPPDVHRSLAWILVARLLGKRLPTIHERVYRDYSLTPAVMRSSDLARRGR
                     GRKMISSARGEVYDLERMFTKLNRRYFDGQIEKPTLTWSQRRTRSILGHHDHVYDSIT
                     ISKTLDSIDVPEWFVEYILYHEMLHIKHPARLINGRRFYHTSAFRTDERRFPHYDQAQ
                     KWLERLARQRRVPRARAA"
     gene            1508..2071
                     /gene="efp"
                     /locus_tag="VFH91_01905"
     CDS             1508..2071
                     /gene="efp"
                     /locus_tag="VFH91_01905"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET6861762.1"
                     /translation="MAIPATQIRRGMVIVFEGQPCKVVEFRHHTPGNLRAMVQTKLRN
                     IRTGTSFEHRFRSADTVDRASLEQHEMEYLYSDGSHHHFMNTENYEQVALSEDDLGDA
                     AQWLTPGLKIQAEFYEGAPIGIDLPPSMELTVTQTEPSLKGATVSNVNKPATLENGVT
                     ITVPPFVNEGDRIRVDPSEGRYIERAK"
     gene            2121..>3062
                     /locus_tag="VFH91_01910"
     CDS             2121..>3062
                     /locus_tag="VFH91_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HET6861763.1"
                     /translation="MYLTYSLLLAVGLLILIPRFIHQALAHGKYLSGLRQRLGSVPEF
                     PHSGKPVIWLHSVSVGETQAARPLIESLRQAYPNHILVVSTITQTGQNLARQILADRA
                     GAVFYFPFDWKWTVRRSLARVKPSLVLLMETELWPNFLKECASQSIPVALVNGRISRQ
                     SFRRYGLIRRFMRRVLHDVSIAIMQTEKDAERIKTLGVPQERLFISGNLKFDSKDNPI
                     ASETIEGLSQRFALSSNVPLILAASTHDPEEKILLESFELVRKTGPARLMIAPRHPQR
                     FEEVASLIQSKGFSWTRRSDVPDPRDSSVDVILLDTIG"
BASE COUNT          771 a          798 c          746 g          747 t
ORIGIN      
        1 acgacggggc atatcacaaa aagttacttc aaaacctggg tttccggcgc gtccgcataa
       61 ccccctaaac ccgagtcagc agtataagga tttaagccag gacctgcaaa caaacgcttg
      121 tcttagagga atttgcgggc gtttcggatc tgatctgtct aaaattgaaa agttagagcc
      181 tgattggcga tgttgagatg taagctcaaa catttcaaca gttacataga cgattcggtt
      241 ttctattcaa gcttcgcaag gttttgaagg cgcctcaagg ctcaactggg cggaaaatcg
      301 agcatgctcc agtttggttg acgagcctgc caacccgtct ttaagattac gtcgctggaa
      361 tttcaaatcc tggtcaaact accggcgctg gttcccgtta gatcaaactt acctcttgat
      421 cctcgcgacc gcaccacagt cccctgagac cagtacagaa tagaatccgc atgagcgatg
      481 tgagcaaatt tttcgctgac gctttccggc atcttggcgg caatcgaggt gtgcccgcta
      541 tcgaggtgcg cttctatccc tacgctggat tgcgacacac catcagaatt cgctcaaaca
      601 gggtgtatgt gcggctttcc gacataacta aagatgctcc tccagatgtt catcgttcgt
      661 tagcgtggat cctggtggct cgattacttg gcaagcgact gccaacaatt catgaacgag
      721 tctaccgcga ctattcccta acacctgcag tgatgcgttc ctcggatctc gccagacgag
      781 gacgtggacg caagatgatt tcatcagctc gcggcgaggt atacgacctc gaacgaatgt
      841 tcacaaaact aaatcgtaga tattttgacg gtcagatcga aaagccaaca ctaacatggt
      901 ctcagcgtcg aactcgaagc atacttggcc atcacgatca tgtttacgac agcatcacca
      961 tcagtaagac tcttgattcc atcgatgtgc cggagtggtt tgttgagtac atcctttatc
     1021 atgagatgct gcacattaag cacccggcga gattgatcaa cggccggcgt ttctatcaca
     1081 ccagcgcctt ccgtacggat gagcgcagat ttccacacta cgaccaggct caaaaatggc
     1141 tggaacgact tgcaagacaa cgtcgagttc cacgcgcccg agccgcttaa cctttggagt
     1201 gcgccggccc ggcggcgctt tgtcacacct ggctcgcact tcgagttctc tcccgcaaag
     1261 ttcgtggaac caacacatca ccccggcgtt actacaaacg aggtaggtgg gctcctcaaa
     1321 aaaagagcgg tctagtccgg ttcaagaacg gactaggccg ctcttttaat acgtttccag
     1381 atgggcgagc tgctggacaa agagtcaaaa gcctagttag actacgcggt ttctcatgag
     1441 ccaatactca ttagagtaga aaccacatta cgtaatagac aactttttaa acggagaatt
     1501 cagttttatg gcgattcctg caactcagat tagacgcggt atggttatag ttttcgaagg
     1561 acagccttgc aaagtagttg aatttcgaca tcacactcct ggaaacctcc gcgctatggt
     1621 gcaaacgaag ttgcgcaata tccgcaccgg aacctctttt gaacaccgct ttcgttccgc
     1681 cgatactgtc gacagagctt ccctcgaaca acatgaaatg gaatatcttt attcagatgg
     1741 ctcacatcac catttcatga acacggaaaa ctacgaacaa gttgctttaa gcgaggacga
     1801 tctgggcgat gctgcccaat ggttaacgcc ggggcttaaa atccaggcgg aattttatga
     1861 aggagcgcca atcggtattg atcttcctcc ctcgatggaa ctaactgtta cgcaaactga
     1921 accctctcta aaaggcgcca cagtttcaaa cgttaataag ccagcaacac ttgaaaatgg
     1981 cgtgaccatc accgtccctc cttttgttaa cgagggggat cgaattcgag tagatccatc
     2041 cgaaggaaga tacatagagc gcgcaaagta gaatcttcaa cttttttgat cgcgctggag
     2101 acacgtccgt ttcgcccgtc atgtacctga cttacagtct gctgctagcg gttggtctcc
     2161 tgatcctaat cccacgtttc attcatcagg cgttggcgca tggcaagtac ctatcaggct
     2221 tgcgccagcg tctcggttcg gttccggagt ttccgcattc cgggaaacca gtcatctggc
     2281 ttcactccgt gtcggttggc gagactcaag ccgctcgacc attaattgag tccctgcgtc
     2341 aggcgtatcc gaatcatatt ctcgtcgttt cgactatcac tcagacggga cagaaccttg
     2401 cgcggcagat cctggccgac cgagcgggtg ctgttttcta ttttccgttt gattggaagt
     2461 ggaccgtacg acgcagtctc gcaagagtga aaccgtcttt ggttttgctc atggaaaccg
     2521 aattgtggcc caactttctg aaggagtgtg cgtcgcaatc aattccggtt gcgctcgtga
     2581 acggcagaat ctcgcgccag tcttttcgac gatacggcct gatccgtcgt tttatgagac
     2641 gagttcttca cgacgttagc atcgccatta tgcagacgga aaaggatgcg gagagaatca
     2701 aaaccctggg cgtgccgcaa gagaggctct tcatttcggg aaatcttaag tttgactcca
     2761 aagacaatcc catcgcgagt gaaacgatcg aggggttgag tcaaaggttc gctttgtcgt
     2821 ctaacgtccc gctgattctg gcggccagta ctcacgaccc cgaagagaaa atcttgttgg
     2881 aaagctttga gttggtcagg aaaacaggac ctgctcggct aatgatcgcg ccgcgccatc
     2941 cgcaacgatt cgaggaggtc gcctcactaa ttcaaagcaa gggtttcagt tggacccgac
     3001 gaagcgatgt accggacccg agagattcga gcgtcgatgt aatcctgctc gacactattg
     3061 gc
//
LOCUS       DASSFP010000091         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1658165, whole genome shotgun sequence.
ACCESSION   DASSFP010000091 DASSFP010000000
VERSION     DASSFP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658165"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1499)
                     /locus_tag="VFH91_01915"
     CDS             complement(153..1499)
                     /locus_tag="VFH91_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861764.1"
                     /translation="MSQTTILTRAEIESMGPPLPEELHDTGIGEGFLCDLALKHVAML
                     PDPTTVAVAERMHLPRTLAEEILQKLYREKLVEVRIQTAVGSTRYAMLDHGWDRLTRL
                     LAVCGYTGAAPVSLNDYAHMMKLQSIPSNPASMEIVRRSFHDLVLPDSLLQTLGCVIN
                     SRRSLFLTGLPGTGKTAVAERINGALAGGIWIPYAVEIDNQIIRVFDSHCHRPLGEDE
                     TPVEYDRRWVLIERPLVVVGGELTLENADLTWSDAAKFYEAPFQMKSNGGTLVIDDFG
                     RQRVAPQDLLNRWIVPLERRVDYLALHTGKKIEVPFEQLVVFSTNLEEKDLADQAFLR
                     RMGYRARVEPPTPAAFSEIFKRQASNRGVTWDPPVMDHLLTKYRVERRQMKGCEPRDL
                     IDRASDICLFESLPLHLTPQILDIAWRNYFGTSHTFAPVATQETMIERTLSDPLEL"
     gene            complement(1518..2495)
                     /locus_tag="VFH91_01920"
     CDS             complement(1518..2495)
                     /locus_tag="VFH91_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6861765.1"
                     /translation="MNKRRIIAILIAFVLTIAATAMWSASTIEREGDPDVNVLEDSGS
                     DAGTTAPEQKKGGNKIVKVLTAPFKAFGSLFKRNNDGKFQRMTEKDAEKFQSVGVERV
                     DYDRNADRKLSAAGSAKEHMESGRRLLLSGQVNEAITELSMAASLDPKLTEAHNLLGV
                     AYDQKGLIDRAKESYEKAVKSNSDDPETLNNLGFSLYKAGNYRAAVDRLKRAAKMAPT
                     DERILNNLGLALCRLGKFDDAYKSFARAGGPLTGNLNIAKMLERFGREDDAIKYYEDA
                     RKIDPNNSMALRRLADLYQRVGKTAEAEEARVALAARTNGNGSVATMDR"
     gene            complement(2507..3439)
                     /locus_tag="VFH91_01925"
     CDS             complement(2507..3439)
                     /locus_tag="VFH91_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6861766.1"
                     /translation="MLLIIGISTFVFITLGMLGVYWLVYRPQSAATERLRSLAVKGGG
                     SANASVIIPEDNPATEIAHRIASPINRLLPPSASEAKKLQKQLMHAGFRSDSAPIVYR
                     AIQLASMAGFPALVALICALLARPLQSALVWIILAFVAGFILPRYSLKRITNKRQRLL
                     RWGLADALDLMVVSVEAGLGLNAAMLKVSSEMRDAHPEIAIELELANLEIRVGRDRDE
                     ALRNLAERTGVDDLRSLVAMLIQTDKFGTSIAKGLRIFSDSLRTKRRQRAEQEAQKAA
                     VKLLLPLACFLFPTLFIAILGPAALNLMDVLGRM"
     gene            complement(3510..>3911)
                     /locus_tag="VFH91_01930"
     CDS             complement(3510..>3911)
                     /locus_tag="VFH91_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6861767.1"
                     /translation="TYEEQNLGLSLKLALENLTERIPLLDLRMCITAVLIQRETGGNL
                     AEILEKVSYTIRERFRIIGDLKTLTTSSRMSAWLLCALPIGVALMVTIMNPDYMSVLW
                     KDPRGHYLIAAALFMQITGMLIVRKILKIQI"
BASE COUNT          950 a         1029 c          966 g          966 t
ORIGIN      
        1 gctcagcttc aacagacctg tcctgatcgg tttttgcaga ctgcgccatc aaaaccgagc
       61 cacttaaact caatacaatt cctacaaaaa agatccggta atagtttaga ttttgatgca
      121 tgctctcctc cggttttgaa cgcggctctg ttttaaagct cgagcggatc ggagagagtt
      181 ctttcgatca tcgtttcctg agttgccacc ggagcgaagg tgtgtgacgt accaaaataa
      241 tttcgccacg cgatatccag aatctgcggt gtcagatgta gcggcaaaga ttcgaacaga
      301 caaatatcgc tggctcggtc gatcaagtct cttggttcgc aacccttcat ctgccggcgc
      361 tcgacacgat actttgtcag caaatggtcc ataactggcg gatcccaggt cacaccacga
      421 ttagacgcct gacgcttaaa tatttctgag aacgcggccg gtgtaggcgg ttcgacgcga
      481 gcgcgataac ccattcgacg aaggaatgcc tggtcggcaa ggtccttttc ctccaggtta
      541 gtcgagaaca cgacgagctg ctcgaaagga acttcaattt tctttccggt gtgaagtgca
      601 agataatcga cgcggcgctc cagtggaacg atccagcgat tcaacaaatc ctgaggcgcg
      661 acgcgttggc gaccaaagtc atcgatgaca agcgtacctc cgttcgattt catctgaaac
      721 ggggcttcgt aaaacttggc agcgtcactc caggttaaat ccgcattctc aagagtcaat
      781 tcaccaccaa caacaaccag cggacgctcg atcagcaccc aacgtcgatc atattcgact
      841 ggtgtttcat cttctccgag cgggcgatga caatgtgaat cgaagactct gatgatttga
      901 ttgtcgatct ctacggcgta cggtatccag ataccaccgg ctaaagctcc atttatgcgc
      961 tctgcgactg cagttttgcc tgttccgggt agtcccgtca aaaacagcga tcgacgtgaa
     1021 tttatgacgc agcctaaggt ctgaagaagc gaatctggca atacgagatc gtgaaatgac
     1081 ctgcgcacaa tttccatgga agccgggttt gaaggaatcg actgcaattt catcatgtgt
     1141 gcgtagtcat taagcgagac aggcgccgca ccggtatacc cgcaaaccgc caggagcctg
     1201 gtaagacgat cccaaccgtg gtccaacatt gcatagcgag ttgaaccgac ggctgtttgt
     1261 atccgaactt caacaagctt ttcccgataa agcttctgaa ggatctcctc ggctaaagta
     1321 cgcggcaggt gcatgcgctc agcaacagcg acggtcgttg gatcgggcaa catggccacg
     1381 tgtttcaggg ccagatcaca taaaaagccc tcaccgatac ccgtatcatg caactcttcg
     1441 ggcaggggcg gccccatact ttcgatttct gcgcgcgtca ggatcgtcgt ttgcgacatg
     1501 tgagttccct tctaagttta tcggtccatg gttgcgactg aaccgttgcc attagttctc
     1561 gcagctaaag cgacgcgtgc ttcttcggct tcggcagttt tgccaacgcg ttgataaaga
     1621 tcggcgagcc gtcggagtgc catcgagttg ttgggatcaa tcttacgagc atcttcgtaa
     1681 tacttgatcg catcatcttc ccgtccaaat ctctccagca ttttggcaat attcagattc
     1741 ccggtcagtg gtccaccagc gcgagcaaag ctcttgtatg catcatcaaa tttgccgagt
     1801 cggcaaagcg cgagaccgag attgttcaag atgcgctcat cggttggcgc cattttggct
     1861 gctctcttca agcggtcgac cgcggcgcgg tagttaccag ctttataaag tgagaaaccc
     1921 aggttgttca gtgtttccgg atcgtcagag ttagacttaa ccgctttctc ataagactcc
     1981 ttggcccgat cgatcagacc tttttgatcg taagcaactc caagaagatt gtgagcttca
     2041 gttagtttcg gatctaatga agcagccatt gagagttcgg taattgcttc gttaacctgg
     2101 ccgctcaaga gcaaccgcct gccgctttcc atatgttcct tcgcggaacc tgcggcgctg
     2161 agttttctgt ccgcgttgcg gtcataatca actcgctcga cgccaacgct ctgaaacttc
     2221 tcggcgtcct tttccgtcat tcgctgaaac ttaccatcgt tattgcgttt gaaaaggctg
     2281 ccaaaagctt tgaatggtgc cgttaaaacc ttaacgatct tgttgccgcc ttttttctgc
     2341 tccggagctg tggtaccagc gtcagagcct gagtcttcca ggacattaac gtcaggatct
     2401 ccctcacgtt cgattgtgga ggcactccac attgcagttg cagcgatagt aaggacgaag
     2461 gcaattagga tcgctatgat tcttcgtttg ttcatgtttg ctccttctac atccttccaa
     2521 gtacgtccat gaggttcagg gccgccgggc caagaattgc gataaaaagc gtcgggaata
     2581 ggaaacaagc cagcggtagc agcaatttta cggctgcttt ctgcgcttcc tgttcggcac
     2641 gctgtcgtcg tttggtgcgc agcgaatcag aaaatattcg cagccctttt gcgattgagg
     2701 taccaaactt atccgtttga attaacatcg cgaccagact ccgaagatca tcaacgccag
     2761 tgcgttctgc cagatttcga agtgcctcat cgcggtcgcg gccaactcga atctcgagat
     2821 tcgccagctc aagctcaata gctatttccg gatgagcgtc tctcatctca gagctcacct
     2881 tcagcatagc cgcgttcaat ccaaggccgg cttcaactga aacaaccatc aagtcaagtg
     2941 catctgcgag tccccaacgt aacaatcgct gtcgtttgtt ggtgattcgt ttcaggctgt
     3001 aacgtggaag aataaacccc gcaacaaacg ccagaatgat ccagacgagc gcgctctgga
     3061 gtggcctggc aagcaaagcg caaatcaacg caaccagtgc tggaaagcca gccatgcttg
     3121 cgagttggat cgcgcggtag acgatcggtg ctgagtctga tcggaaacca gcgtgcatca
     3181 attgtttctg cagcttcttc gcttcactcg cagaaggagg cagtagacga ttgattggtg
     3241 aagcaatccg gtgagctatt tcggtggccg gattatcttc aggaatgatt accgaagcat
     3301 tagctgaacc accgcctttt acggccaggc ttctcaaccg ttcagtggcc gcgctctgcg
     3361 gacgatagac cagccagtaa acacccaaca tacccagggt gataaaaacg aaagtggaga
     3421 tcccgattat tagcagcatt ggagtgttcg gtctttcgtc ttggttccgg ctcgccgtca
     3481 attcatgagc aggccggacg cacgttgtgt tagatctgaa tctttagaat ctttctaacg
     3541 ataagcattc cggttatctg catgaacaga gcagccgcga ttaagtagtg accacgagga
     3601 tctttccaca aaacgctcat gtaatcaggg ttcatgatag tcaccatcaa cgcaacgccg
     3661 atcggcagag cgcagagtaa ccacgcagac atacgcgaac tcgttgtcaa cgttttcaaa
     3721 tcgccgatga tgcggaagcg ttcacgaatg gtgtaggaaa ccttctcaag aatttcggcc
     3781 agattaccgc ccgtctcacg ctgaatcagg accgccgtga tacacatgcg aaggtccagc
     3841 aagggaatac gttccgtgag attttcgagt gctaacttga gcgataaacc aaggttctgc
     3901 tcttcgtaag t
//
LOCUS       DASSFP010000092         6346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_169245, whole genome shotgun sequence.
ACCESSION   DASSFP010000092 DASSFP010000000
VERSION     DASSFP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169245"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFH91_01935"
     CDS             <1..208
                     /locus_tag="VFH91_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861768.1"
                     /translation="GRLLRADTLVSLPDAFAKIAAELRTQYLLGYYPTNKEPTNQYRK
                     IAVKTSRKDVAIRSRPGYVSKETR"
     gene            291..863
                     /locus_tag="VFH91_01940"
     CDS             291..863
                     /locus_tag="VFH91_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6861769.1"
                     /translation="MPDRAEAWELLCEYTKGDSLRKHALAVEAAMRASAKKYGSTDAD
                     VDEWGIVGLLHDFDYEMFPSADQHPFTGSNILCGRGYPERIIRAILGHATYTGVPRDT
                     DMARALFATDELCGFLVACALVRPSRSLDDMEVASVKKKLKDKAFARSVNRDDIRQSV
                     AEMGIDLDEHIRFVIDALRPLQKEIGLNPI"
     gene            complement(872..1210)
                     /locus_tag="VFH91_01945"
     CDS             complement(872..1210)
                     /locus_tag="VFH91_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861770.1"
                     /translation="MRLKLLIVASMLAAIVGAGSCITIVFLAFASLKPFTTPGLLVLA
                     TFLLPTASIIFAAIFVYRHTARRRKLQAALTVLIATLITICLFVLASIISARRDTIEP
                     PQPAGPRIAS"
     gene            1374..2294
                     /locus_tag="VFH91_01950"
     CDS             1374..2294
                     /locus_tag="VFH91_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET6861771.1"
                     /translation="MLNRSALIWLSRQEGLKDFATRFRPFKKLTTRFVAGETIDEVIP
                     FIREINSEGSGASFDHLNESVTSAAEAEAEVAEYLHILEKIVETGIKSNVSIKLTQFG
                     LGLDPDLAYKNARRVVVEAQKLGIFVRIDMEDSSVTQITLDIFKRLRAEFGLNDVGIV
                     LQSYLRRTYSDAEDLLKIPARIRICKGAYNEPEEVAFPEKRDVDANYVKVMQLLLASG
                     VYHGVATHDPKMIEATLDFVRRKGVGKDQFEFQMLYGIRRDLQRKLAHDGYNVRVYVP
                     YGKHWYPYFMRRLAERPANIWFVLKNLLKG"
     gene            2342..2833
                     /locus_tag="VFH91_01955"
     CDS             2342..2833
                     /locus_tag="VFH91_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET6861772.1"
                     /translation="MDDWIWILWAVLGAALIVAEIFTSGFVLLWFGIGALVASIAGLA
                     GLNSLALQFLIFAVVSISLTAASRTVFANLFTRGLMGQSLKSGVDALPGKIGTVVTSS
                     EGALQEGAVKVFGSTWTAYPAAGEEPLVAGERVLVESVEGASIYVRRVGAEPDWRRRE
                     LDS"
     gene            complement(2843..3772)
                     /locus_tag="VFH91_01960"
     CDS             complement(2843..3772)
                     /locus_tag="VFH91_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET6861773.1"
                     /translation="MIVELFAKSDVGRVRRGNEDNFLVLDLSTEQTWTAADGAVPPQK
                     LTRFDLGQKGLVLVVSDGMGGALAGDVASRMAVDSVREMLIGNDSTENCDPSADLVDC
                     LKNATIYANLAIHVKSQEDSRCAGMGATFTGAAVKGDSLDLVQVGDSRGYLIRREQIR
                     LATKDQSLVQQLVDVGQISESEAETHMFRNVILQALGAQSEVSPVTGRIRLRQGDILL
                     LCSDGLSGKLRADDIQRIVDSSNGDLVIACDLLVEEANNRGGEDNITVVLARFLGDDL
                     APPTTDKITIELPPLEEDKTLDDNYEADTEPQG"
     gene            complement(3852..4994)
                     /locus_tag="VFH91_01965"
     CDS             complement(3852..4994)
                     /locus_tag="VFH91_01965"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET6861774.1"
                     /translation="MGFATIAIHTGSEPDEATGSVTVPIYQTSTYAQDALGKNKGYEY
                     ARTQNPTRSALERNIAALEGARFGFAFASGMAAIDATLRLVKAGEHVVVSDNTYGGTA
                     RLFTRILANYNLEFDFVDTSDPANVESALKQNTRMVFVETPTNPVMIVTDLKEVSEIA
                     HRAGARVVCDNTFMSPYLQRPLEFGVDIVVHSTTKYLNGHSDGVGGMVVLNDEEDANW
                     IGFVQNSAGAILSPFDSWLVMRGTKTLALRMEQHDRSGRAVAAFLEEHPKVKKVYYPG
                     SVSHPQHALARRQQHGFGGMVAFDVGSLAAARTVLESVKLCTLAESLGGVETLISHPA
                     TMTHASVDAAKRERLGITEGLVRISVGIEDTDDIVADLDQALDKIV"
     gene            complement(5026..5952)
                     /locus_tag="VFH91_01970"
     CDS             complement(5026..5952)
                     /locus_tag="VFH91_01970"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET6861775.1"
                     /translation="MAASVAESVIDLIGRTPLIHLSRFAPPPLADVFAKIEWFNPGGS
                     VKDRAALGMILDAEDRGLLSRGSTIIEPTAGNTGIGLALVGVARGYRVILCVPRGFAE
                     EKMKLIEVLGGEIEYVSHDAGMKGAIERSHQLASTIENSFVPQQFENKANPCFHERTT
                     AQEIIAQMDGDIDAVVIGAGTSGTFVGVSRAVRNINKNVLCVLVEPEGSIYGGHPPGP
                     KKVEGIGQRDFIPEIFDPTTADEIMMVEDEEVCAALKKVARLEGLLGGWSSGAAAAAA
                     HKVAKRLGKGKRIVTVFPDGAERYRSQGIFDE"
     gene            complement(6151..>6346)
                     /locus_tag="VFH91_01975"
     CDS             complement(6151..>6346)
                     /locus_tag="VFH91_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861776.1"
                     /translation="SKLLPATYGLDGIRQVLLEGHNLSQVTQPLITLLIFLTVLLPFS
                     LWVFGRAVRKAKREGSLIQY"
BASE COUNT         1590 a         1611 c         1570 g         1575 t
ORIGIN      
        1 tggacgtttg ttgcgcgctg atacgctggt gtcattacct gatgcgtttg cgaagatcgc
       61 tgccgaactt agaactcaat acctgctcgg ttattatccg actaacaaag aacccactaa
      121 tcagtaccgc aagattgccg taaagacttc acgtaaagac gtagcaatcc gatcgaggcc
      181 tggctatgtg tcgaaggaaa ctcgttaaat cctgttcttg ctgcccgcct gaagccgtgc
      241 tagttttgtc atcgttctgg gtaatttaaa ttccaccttc aatactgaac atgcctgatc
      301 gcgcagaagc ctgggagttg ttgtgtgagt acaccaaggg cgatagtctg cgaaaacatg
      361 ctctggcagt cgaagcagca atgcgagcaa gtgctaaaaa gtacggctca acagatgcag
      421 acgtagacga atggggtata gtcgggctgt tacacgactt cgactacgag atgtttcctt
      481 ccgcagacca acatccgttt accggttcca acatactttg tggacgtggt tatcccgaaa
      541 gaattattcg agcaattctg ggccatgcta catacacagg agttccacgt gatacagaca
      601 tggctcgagc actcttcgcg acggatgaac tctgtgggtt tttagtagcc tgcgcacttg
      661 tgcgaccgtc acgaagtctt gatgacatgg aggtagcatc cgttaagaag aaactgaagg
      721 acaaggcatt cgcacgttca gttaatcgag atgatatacg tcagagcgtt gcagagatgg
      781 gcatcgatct tgacgaacac ataagatttg ttatcgacgc gcttagacca ttgcaaaaag
      841 agatcggttt gaatccaatc tgatgagtga ctcaggaggc gatgcgcggt ccagcaggtt
      901 ggggtggttc gatggtgtct cgccgtgcgc tgataattga ggcgagcaca aatagacaga
      961 tcgtgatgag tgtggcgatg agaaccgtaa gtgccgcctg taactttcgc cgtcgcgcgg
     1021 tatgacgata gacaaagatg gccgcaaaaa taatagaagc cgttggcaga agaaacgtag
     1081 ctagtacgag cagccctgga gtggtgaagg gtttgagaga agcgaatgct agaaacacaa
     1141 tcgttatgca ggatccagcc cccacgattg ctgcaagcat cgaagctact atgagaagct
     1201 tcaaccgcat tgaggaactt cctttgttcc gatatgttcg accaagaacc tctcctcagg
     1261 ctggcaggtg aaaggcgccg ccaaggatac cacagctgac gcgaggcatg acgatctttg
     1321 gcttgggcca ccgatagccc aaaagccgac taaaatactg attacaattg ctgatgctaa
     1381 acagaagcgc gctgatatgg ctgtcacgtc aagaggggct caaggatttc gccacccgtt
     1441 ttcgaccatt caaaaaactg accacccgtt tcgtcgccgg agaaacaatc gatgaagtaa
     1501 ttccgttcat tcgcgaaatc aattccgaag gttccggcgc ttcgttcgac catctcaacg
     1561 aatctgtcac aagcgccgcg gaagcggaag cggaagtcgc tgaatatctt cacatcctcg
     1621 aaaagattgt tgagactggc atcaagtcga acgtctcgat caagctgaca caattcgggc
     1681 ttggcctcga tcccgacctg gcctacaaga atgcgcgacg agtggtagtg gaggctcaga
     1741 agctgggcat ctttgttcgt atcgatatgg aagactctag cgttacgcag atcacgctgg
     1801 atatcttcaa gcgactgcgc gccgagtttg gtctaaatga tgttggaatc gttctgcaat
     1861 cttacctgcg acgaacctat tctgatgccg aagacttatt gaagattccc gctcgcatca
     1921 gaatttgtaa gggcgcttat aacgagcctg aagaggtggc gtttcctgaa aaacgagatg
     1981 tcgatgcaaa ctatgtgaag gtcatgcagt tattgctggc cagcggcgta tatcacggtg
     2041 tagctactca cgatccaaaa atgattgagg ccacactcga tttcgttcgc cgcaagggcg
     2101 tcggcaaaga tcaattcgag tttcagatgc tttatggaat tcggcgtgat ttacaacgaa
     2161 agctggccca tgacggctac aacgtgagag tgtatgttcc ttatgggaaa cactggtatc
     2221 cttacttcat gcgccggcta gcagaacgtc cggctaacat ttggttcgtg ctcaagaatc
     2281 tgttgaaggg ttgacaaggt caccgcattg cgacgcaagt cactctggag actaactata
     2341 aatggacgac tggatttgga tcctgtgggc cgtattagga gccgccttaa tcgtcgccga
     2401 gatttttact tctgggttcg tattgctatg gttcgggatt ggcgcattgg tggcctcaat
     2461 tgccggactt gctggtttga atagtctcgc actgcaattc ctgatattcg cggtagtttc
     2521 gatcagtttg acggcagcct cgcgtaccgt atttgcaaat ctgtttaccc gcgggttgat
     2581 gggccaatcc ttgaagtccg gagtggacgc attacccggt aagatcggaa ccgtggttac
     2641 ttcaagtgaa ggagcgttgc aggaaggcgc cgtgaaagtg tttggttcga cctggactgc
     2701 gtatccggcc gctggagaag agcctttggt agccggcgaa agggttttgg ttgaaagtgt
     2761 tgaaggagcc tcgatctacg tgcgtcgagt tggtgcagag cctgactggc gtagaaggga
     2821 actggatagt tagttggctg aactagcctt gaggttcggt atcggcttcg tagttatcgt
     2881 ccagcgtttt gtcctcttca aggggaggca attcgatagt gattttgtca gtcgtgggcg
     2941 gagcgagatc gtcaccaaga aatctggcga gcaccactgt aatattgtct tcaccacctc
     3001 gattattagc ctcttccact aatagatcgc aggcaatcac cagatcgcca ttactactgt
     3061 ctacgattct ttgaatatcg tccgcacgca acttacctga caaaccatcg ctgcaaagta
     3121 gaagtatgtc accttgtctc aagcgtattc gtccggttac aggtgaaact tcactctgtg
     3181 cgccgagagc ctgaagaatg acattgcgaa acatgtgagt ttctgcttcg gattcgctga
     3241 tctggcccac gtcgactaac tgctgaacca gtgattggtc ttttgtcgca agacgtattt
     3301 gctcgcggcg gatcaggtaa cccctactat ctccaacctg gaccagatcg aggctgtcgc
     3361 cctttaccgc cgccccggtg aacgtggctc ccattccggc gcaacgagaa tcctcctggc
     3421 tcttaacgtg gatcgccaaa ttggcgtaga tggttgcgtt ctttagacaa tcaaccagat
     3481 cggcacttgg atcacaattt tcagtactgt cattgccgat tagcatttct cgcacagaat
     3541 cgacggccat gcggctcgca acatcaccgg caagcgcacc acccatgcca tcggagacga
     3601 ccaagaccag gcctttttga cccagatcaa aacgagtcaa tttctgtgga ggcactgcac
     3661 cgtcggcagc ggtccatgtc tgttctgtgg aaaggtcaag aaccagaaaa ttgtcttcgt
     3721 tgccacgtcg gacacggcca acatctgatt tggcgaacaa ctcaacaatc atatggagag
     3781 cgaaataaag gaatcagtga ttaggctgct gagaacgcgc aattgtaacc cttctgcagt
     3841 ttacctgtca ctcaaacaat tttgtcgagt gcttgatcta aatccgcgac aatgtcgtca
     3901 gtgtcttcaa taccgacaga aattctgacg agcccttctg ttataccaag tcgttctctt
     3961 tttgccgcat cgactgaagc atgagtcata gttgccggat gagaaatcaa agtctcaacg
     4021 ccgcccaggc tttcagccag tgtacagagc ttaacagatt caagcacggt acgcgctgct
     4081 gctagcgaac caacatcgaa cgcaaccatt cctccaaacc cgtgttgctg ccggcgtgcc
     4141 agtgcatgct gcgggtggga aacggaacct ggatagtaaa cctttttaac tttcggatgt
     4201 tcttcaagga atgccgcgac ggccctaccg gaacgatcgt gttgctccat tctcagtgcg
     4261 agcgtcttag tccctctcat tacaagccat gagtcaaagg gtgacagaat ggctcccgca
     4321 ctgttttgca cgaaacctat ccagttagca tcttcctcat cattcaggac caccatgccg
     4381 ccaacgccgt ctgaatgccc atttagatac ttcgttgtcg aatgcaccac tatatcaacg
     4441 ccgaattcca acggtcgctg tagatacggc gacataaacg tattgtcaca aacgacgcga
     4501 gctcctgcgc gatgagcaat ctcgcttact tctttcaaat ccgtcacgat cattaccgga
     4561 ttggtcggtg tttccacaaa gaccatccgc gtattctgtt tcagcgctga ctcgacattc
     4621 gcgggatccg aagtatctac gaaatcaaat tcgagattgt agtttgcgag tattcgtgtg
     4681 aacagacgtg ccgttccacc ataagtgtta tcggaaacga caacgtgttc gccagcctta
     4741 acgagtcgga gcgtcgcatc aattgccgcc atgccggatg cgaacgcaaa tccgaagcga
     4801 gctccttcca gcgctgcgat gtttcgctcg agtgcggagc gcgtaggatt ctgcgtcctc
     4861 gcatactcgt agcctttatt tttgccaaga gcatcctgtg cgtacgtaga agtctggtaa
     4921 atgggaactg tcactgaacc agttgcttca tctggctcag atccggtgtg aattgctatt
     4981 gttgcgaatc ccataatcaa ataaatagtg aactgatttt aagatctatt cgtcaaaaat
     5041 cccctggctt ctgtagcgct cggccccatc aggaaatacg gtcactatcc gtttgccctt
     5101 acctagtcgc ttagccacct tgtgtgcggc agctgctgca gctcctgagg accaaccgcc
     5161 caatagccct tccaatcgtg caactttttt tagcgcggca caaacttcct cgtcttccac
     5221 catcatgatc tcatcggccg ttgtgggatc aaaaatttcc ggtatgaagt cacgttgacc
     5281 aataccttca actttttttg gcccgggcgg atgtccaccg taaatcgagc cttcgggttc
     5341 taccagaacg caaaggacgt ttttatttat gttccgcaca gctctggaaa cacccacaaa
     5401 cgttcctgac gttcctgcac caataacaac ggcgtcgata tcaccgtcca tttgagcgat
     5461 gatctcctgc gcggtcgtcc gctcatgaaa gcacgggtta gctttattct caaattgctg
     5521 gggaacaaaa ctattttcaa tagtgcttgc caactgatgc gaccgttcga tggctccctt
     5581 cattcccgcg tcgtgagaaa cgtattcaat ttcaccgccg agaacttcga tcaatttcat
     5641 tttctcttcc gcaaaaccgc gcgggacgca gaggataaca cgatatcccc gtgccactcc
     5701 taccagcgcc aacccgatcc ccgtatttcc cgcagttggc tcaattatcg ttgagccacg
     5761 acttaacagt ccacgatctt ccgcgtcgag aatcattccc agcgccgcac gatccttaac
     5821 tgagccacct ggattaaacc attcgatctt tgcaaagaca tcggccagtg gtggaggggc
     5881 aaaacgggaa aggtgaataa gcggggtgcg accgataagg tctataacgc tttctgctac
     5941 gctggcagcc attaattaaa ttgcggtaaa tacagttaag gaagatccaa caaggtcaaa
     6001 aagcgatggt agcccgcgag ttataccctg tcaattaagt tgcaattatt ggggcctccc
     6061 tccgtgggcg cccattcttt tggtgtcaac tcaattcata tgaccgctac gccatatttg
     6121 agcgcccacg aagagacgcc cctacaaatg ttaatactga attagacttc cttcgcgctt
     6181 ggcctttctg acagctcgtc caaaaaccca caaagagaag ggaagaagca cagtgagaaa
     6241 aatcagtaat gtgatcagcg gttgcgtcac ctgactcaaa ttatgacctt cgagtaatac
     6301 ctgacgaata ccgtcgagtc catatgtggc cggcagcaac ttcgaa
//
LOCUS       DASSFP010000093         5851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1506301, whole genome shotgun sequence.
ACCESSION   DASSFP010000093 DASSFP010000000
VERSION     DASSFP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506301"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1984)
                     /locus_tag="VFH91_01980"
     CDS             complement(92..1984)
                     /locus_tag="VFH91_01980"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HET6861777.1"
                     /translation="MEPFKDLLETDRIHVFDGAMGTMLYAKGVYINRCYDELNLSNPE
                     LVRDVHSEYIRAGADIIETNTFGATAFKLQQYGLEANLHEINAIAARVAREAAADRAY
                     VAGAIGPLGLRIEPYGPTSFDEAKEMFKAQAIALLEGGVDLFCLETFSDVSEIRQAIR
                     AIRELCDLPIIAQMTIMIDGNTQFGTTPEVFTQRLDEWGADVIGLNCGVGPAIILNGL
                     DKMRAVTKKKLSAQPNAGLPRDVQGRQFYMCSPEYMSKFTKRFIQSGAKFIGGCCGTT
                     PAHIKLITDAVRAASPRAQRLALSNVAPAHVTEITPADIKVVPPEERSRWGRKIAKGQ
                     FVTSVEVLPPKGVDSKKTLDSIRLLKDAGVDGVNIPDGARAQSRMSALATAVLVEQQI
                     GIEAVLHYCCRDRNLLGMMSDLLGAAGLGLRNILVITGDPPKMGPYPDATAVFDIDSI
                     GLTNMVNKLNHGLDLGNNPIGEPTAFCIGVGVNPGALNLDEEIKRFEWKVEAGAQYAI
                     TQPVFDTQQLRDFLKRIEHVRIPVIAGIWPLVSYRNAEFLHNEVPGVRVTPTIMDRMR
                     NASAISKEAGRDEGLKIARESLLEVRDVIQGVQVSAPFGNVKYALEVFGALDDFGELE
                     VASQTS"
     regulatory      complement(2225..2300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            2766..3377
                     /locus_tag="VFH91_01985"
     CDS             2766..3377
                     /locus_tag="VFH91_01985"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6861778.1"
                     /translation="MKLPRNVTLLRPIKFTPLALIGLFLFSNFSDVLLSHALAPLVSA
                     QTTGIPSDIPTSGDPNLDAIILKVGESEGVDPRFIHAVIWQESRYETTARSHAGAQGL
                     MQLMPATAKRFGCKDVNDPTDNITAGTKYLSWLLKRFSGDVGLTLAGYNAGEGSVDKY
                     NGVPPYDETRNYVKVITERYGKSYHPVLAPEEAIQVFHLQTTE"
     gene            complement(3510..3839)
                     /locus_tag="VFH91_01990"
     CDS             complement(3510..3839)
                     /locus_tag="VFH91_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861779.1"
                     /translation="MANESPFACDMTAIAPQKRERHIATIKRLFRSVTYVNELPNGYK
                     FELPNQTETLELAAEFICLERLCCPFFGFTLDVEPEGGKLLLSLTGREGVKPFILEEI
                     GEHLRKG"
     gene            4159..5679
                     /locus_tag="VFH91_01995"
     CDS             4159..5679
                     /locus_tag="VFH91_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HET6861780.1"
                     /translation="MSSSREATVTPDTHGFFGHPKGLSTLFFTEMWERFSYYGMRALL
                     TLYMTKSLADGGLGFDEKFAGIIYATYVSSVWYLPLIGGWLADRFLGARRAVLLGGIV
                     IACGHYSMAIDSRATFYAGLILIAMGTGLLKPNISAMVGQLYPPEDLRRDAGFSIFYM
                     GINLGAFFAPLITGFLAQSATIRGFITSLGMDPNKSWHWGFAAAGVGMTLGVIQYLIG
                     GNRLSHVGVKPEKVSANETSAKFDSLTTILAVGGALLGVAFGYKFGEGGWISVPFWSV
                     VGYFAGYLTGTTRLLESEERKRVLVIFILVMFSIVFWMTFEQAGSSLTLFADRLTRNS
                     IFGLQFPSSWFQSVQPIFIIALAPVFAFIWQTLGDRQPSSPAKFALGLFLAGSAFVVV
                     AFASTLTGQGLVSPLWLVAVYFFQTLGELCLSPVGLSTISKLSPARMVGMMLGVWFLS
                     IAIGSYIAGLTTRLFEGSSPEVLAKAFGTFAAISLAAALLLAVLTPLIRKMIPKES"
BASE COUNT         1390 a         1456 c         1439 g         1566 t
ORIGIN      
        1 tttaagacgc cgaccaagaa gaggacgaag aaatcgacga atagtaagtc gaatggttaa
       61 gagttgtgag ggcggccacg gagggccgcc cctacgaggt ttgcgaggcg acttccaact
      121 ctccgaagtc gtcgagagcg ccaaagactt ctaaggcgta cttcacattt ccaaatggcg
      181 cggagacttg tactccctga ataacatcac gaacttcgag cagcgattct ctggcgatct
      241 tcaatccttc gtcgcggcca gcttccttac tgattgctga cgcgttgcgc atcctgtcca
      301 tgatggtggg tgtgactcga actcctggta cctcattgtg caggaattcg gcgtttcgat
      361 aagagaccaa cggccaaatt cctgcaatga cgggaatacg aacatgttca attcgtttta
      421 gaaaatcacg caattgctgc gtatcgaaaa caggctgcgt aatcgcgtac tgagcacctg
      481 cctcgacttt ccattcgaat cgtttgatct cctcatccag attcagagca ccaggattca
      541 cgccgactcc aatacagaag gccgtgggct cgccgatggg gttatttccg aggtcgagcc
      601 catggttcaa tttgttgacc atgtttgtca gaccaatcga gtcgatatca aaaaccgccg
      661 tcgcatctgg atatggtccc atcttcggtg gatcaccagt gatgaccagt atgtttctaa
      721 ggcccagacc agctgcgccc aaaaggtcgg acatcattcc cagtaaattg cgatcgcgac
      781 agcaataatg caatacggct tcgataccta tctgttgttc cacgagtacc gctgtagcga
      841 gcgcactcat tcgcgattgt gctctcgctc cgtctggaat gttgactcca tccacccccg
      901 cgtctttcag caagcgaatc gagtctaacg tcttctttga gtcaacacct ttaggaggca
      961 ggacctcaac tgaagttaca aactgtcctt tagcaatctt tcgtccccac ctcgatcttt
     1021 cttcaggtgg gactactttg atgtcagcag gtgtaatttc tgtgacgtgc gccggcgcta
     1081 cgttactcag agccaggcgt tgagctctgg gagacgctgc ccgaaccgcg tccgtgatca
     1141 atttgatgtg agccggcgta gtgccacaac aaccaccaat gaacttagcc ccggactgaa
     1201 taaacctctt ggtaaacttc gacatgtact ctggcgagca catataaaac tggcgtcctt
     1261 gaacatcgcg gggaagaccg gcatttggtt gagccgacaa tttcttcttc gtgacagctc
     1321 tcatcttgtc gagcccattt aagataattg ccggaccaac gccgcaattc agcccaataa
     1381 catcggcgcc ccattcatcg agacgttgag tgaacacctc gggcgtggta ccaaactgcg
     1441 tgttgccgtc tatcatgatt gtcatctgcg caataatcgg caggtcgcat agttctcgaa
     1501 ttgcgcgaat ggcttgtctg atttcggaaa cgtcggaaaa ggtttcgagg cagaagagat
     1561 ccaccccacc ttcaagcagt gcgatggctt gagctttgaa catctctttc gcttcgtcga
     1621 aagaggtcgg cccgtatggc tcgatacgta gccccagtgg tcctatggct ccggctacgt
     1681 acgctcggtc ggcagctgct tcgcgcgcaa cgcgagcagc aatggcgttt atttcatgaa
     1741 gattggcctc gagtccgtac tgctggagtt taaaagctgt cgcgccgaac gtgttggttt
     1801 cgataatgtc ggcgcccgct cgtatgtatt ctgaatggac gtctcgaacg agttccggat
     1861 tgcttagatt caactcgtca taacagcggt tgatgtacac acctttcgcg tagagcattg
     1921 ttcccattgc tccgtcgaag acgtgaattc gatctgtctc gagaaggtct ttaaaaggtt
     1981 ccattgactt gatttttatg ttgtccgtcg tcgtccggtg cactttgcgc tttgtacttc
     2041 gcgctttgaa cttcgtggtt tgaatcgcaa gtaattacgg tcattactgg tttaatcatt
     2101 ccgacgaaga tacaagtctc agctgtcgac aacaaagtac aaagcacaaa gtacgagcac
     2161 aaatataaaa gtcccgcgcc aaacaacgcg ggacttcaaa atcgtttgaa gtttttcggg
     2221 taaaggttcc agttgcccga gctgtttagc tggttatttt acgtggccgc aagtcgcctt
     2281 aactcaccac gcttgtcgct ttcgcttata cgcctagcct gtcagcgtgt caagaacggg
     2341 ttggtccgtt gtccgttgtc cgttgtccgt agtccgttgt ccgttgcagt gggtaagaag
     2401 catgccccgc cagaactagc aacgcggagc agtgaacaac tgacaacgga caactgacta
     2461 cggacaagtc tcaaaagtgg gacttctgtc acaaagttgt gttcgctttt acgggtcagg
     2521 ctgattgaat ccttcaatga gaccgattat cgaaccgaat aagtacattt gaaatcatat
     2581 acttacaaga atataatgca aagaaaaggg caactgattc tgagactcca gacatcagac
     2641 tccgtggcac gcaaattaag ctgcgagcca ccctttgctc agttttcggc gcgtttgcac
     2701 gtcgcccagt agacgtagtt caggcgcgta atagataagc cctttaactg ttggagaata
     2761 tacgaatgaa gttgccccgg aacgttaccc ttctgcgccc aatcaagttc accccattag
     2821 ctctcatcgg tttgttcttg ttttcaaatt tcagcgacgt ccttttgagc catgcgttag
     2881 cgcctctggt tagtgcccag accaccggaa taccgtccga cattcctacg tcgggcgatc
     2941 cgaacctgga cgcaataatc ttaaaggtgg gcgaaagtga aggagttgat ccgcgtttca
     3001 ttcatgctgt gatctggcaa gagtcgcgat atgaaacgac cgctcgttct cacgctggag
     3061 ctcaagggct gatgcagctg atgccggcca cggctaagag atttggttgc aaggacgtta
     3121 atgatccaac cgataacatc acggccggaa ctaaatattt aagctggtta ctcaagagat
     3181 tttcggggga cgtaggttta actttagccg gttacaacgc cggtgaagga tcggtcgata
     3241 agtacaacgg cgttccgcct tacgacgaaa cgcgcaacta cgtcaaagta attaccgaac
     3301 gctacggcaa gtcctatcac ccggttctgg caccggaaga agcaatccag gtattccatt
     3361 tacagacgac ggaatagcgt aggcatggga cagaccacat caaatgatca attatgaatt
     3421 gagatttgat aattattatt tgcttaagac gtgggccaac ttacaacggg cgtctcatta
     3481 aataataatt atcaaatctc aattcataat tatccctttc tcaggtgctc tccaatctcc
     3541 tccagaataa atggtttcac accttcacgt ccggtgagac tgaggagcag tttgccaccc
     3601 tcgggttcaa cgtccagagt gaagccgaag aacggacaac atagtcgttc cagacaaata
     3661 aactccgccg ctaattccag tgtctctgtt tgattcggca actcgaactt atagccgttc
     3721 ggtagttcat taacataagt aactgatctg aataatcgct tgattgtcgc aatgtgccgc
     3781 tcacgtttct gaggcgcgat tgctgtcata tcgcacgcaa aaggcgattc gttagccatg
     3841 agttaactcc tttttaggta acatctccgt gtaaccttcg tgacctctgt gcctcggtgg
     3901 cgagcaaagt cttaagataa cgataaccac agagtcacag agatctcaga gaaagcacag
     3961 agaaaactca gattaggata ctactcaatg cttgccctgc ttgacacttg tctgactatt
     4021 ttggctgaat gcacgcggtc gtccagtcta cgttcaccta aatcagaaat ttgaacaccg
     4081 gcattcaggc tctctaagat ctgctccgat tctgcctggc ctgccggtcc atcccggaca
     4141 aatcgaggaa cgcgattaat gagctcatct cgagaagcaa cagtcacacc cgacacacac
     4201 ggtttctttg gccatcctaa aggactctcg actctgtttt tcaccgagat gtgggaacgg
     4261 ttctcatatt acggaatgag ggcgctcctt acgctttaca tgacgaagtc gctcgcggat
     4321 ggaggtctgg gcttcgacga gaaattcgcg ggaataatct acgccacata cgtgtcatca
     4381 gtctggtacc tgcctttgat aggaggatgg ttggctgaca gattcctggg agcccggcgc
     4441 gctgtgctcc tcggcggtat cgttatcgca tgtggccatt actcaatggc gattgattct
     4501 cgcgcgacgt tttatgctgg tctaattttg atcgcgatgg gaactggatt gcttaagccg
     4561 aacatcagcg cgatggtcgg ccaactctat ccacctgaag atttgcgacg cgacgcaggc
     4621 ttctccattt tttatatggg aattaacctc ggcgctttct ttgctccatt gatcacgggt
     4681 ttcctggcac agagcgctac gattcgaggt ttcattactt cactgggtat ggatccgaat
     4741 aagagttggc actggggatt tgcggccgca ggtgtcggga tgactctcgg agtgatccag
     4801 tatctaatcg gcgggaacag acttagccat gttggtgtga aacccgagaa agtttcagca
     4861 aacgaaacat ctgcaaaatt cgattctctc accactattc tggcggttgg aggtgcgttg
     4921 ttaggcgttg cgttcggata taagtttggc gagggcggat ggatctccgt cccgttctgg
     4981 agcgtggttg gttattttgc cggatacctt actggcacga ccagattgct tgaaagcgaa
     5041 gaacgcaagc gagtgctagt catatttatt ctcgtgatgt tctcaatagt tttctggatg
     5101 acgtttgagc aagcggggtc gagtttgact ctctttgccg atcgtttgac ccgtaattca
     5161 atcttcggac ttcagtttcc atccagttgg ttccagtcgg tccaaccgat atttattatc
     5221 gcgttggctc cagtgttcgc cttcatctgg cagactttgg gcgatcgcca gccctcgagt
     5281 cccgcaaaat ttgcgcttgg tctgtttctg gcaggctcag catttgtggt tgttgccttt
     5341 gcctcaacgt tgaccggaca gggattggtt tcgcctttgt ggcttgttgc tgtgtacttc
     5401 ttccaaactc tcggcgagtt gtgtctgagc ccggtcggat tgagtacgat ttctaaactt
     5461 tcgcctgcgc gtatggtagg aatgatgctt ggagtctggt tcctgtctat tgccatcgga
     5521 agttacattg cgggactaac aacgagactg tttgagggga gtagtccgga ggttcttgct
     5581 aaagcattcg ggacttttgc ggccatctct cttgcagctg ctttactcct ggcagtactg
     5641 acacctttga tcaggaagat gataccgaag gagtcataaa agtcatccaa gtgctacatg
     5701 gacagtctaa aaagcgattc acaatatttg cgacaatgat actgactgtg ccaacccttg
     5761 cttcatagat tttttacatt cgattcatag gagaatattc tgagaatgcg gttctcatta
     5821 cttcctgctg gtctattaat actcctttca g
//
LOCUS       DASSFP010000094         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1062789, whole genome shotgun sequence.
ACCESSION   DASSFP010000094 DASSFP010000000
VERSION     DASSFP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062789"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFH91_02000"
     CDS             <1..1010
                     /locus_tag="VFH91_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861781.1"
                     /translation="LMVVSGGVWQIPLSVDGEPVGKPEQIANPAMAQVRNLSFSADGK
                     RVVSSVVRQKGNLWSVPISKTSGERMGQPAPFVEDTSSRKTNPIFSNDGSKVAYTVWI
                     AGFAGSVWTVGEKGNDRFQVTTDPSSIVGWMPEGNRLVSISFHDDDNYLTTTAVDTGM
                     RKTLAKLHRHLPFCRLSPDGKTIAFNAYSDSNVNLWSYSLESETEKQLTFDHELFGFP
                     AWSPDGKWIAGELKRKEDTYVAIVPAEGGAPVQLNSDQGQSWTGSWSPDGDKIAFAGL
                     RNGVWNIWWISVSTREQKQVTNYTKPNSFVRYPTWSPRGDQIVYEYSELTGNVWLADL
                     K"
     gene            1204..1764
                     /locus_tag="VFH91_02005"
     CDS             1204..1764
                     /locus_tag="VFH91_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6861782.1"
                     /translation="MQQNEGVTQLLVDWGKGDKEALERLMPLVYSELRRLATNYLRRE
                     RPGHTLQPTALVNEAYLRLVDQKNAQWQNRAQFFGISAQLMRRILVDHARQKQAAKRG
                     GSGQQRLSISSAHAFVDGPGIDFLALNEALEELSKMDAQQERIVELKFFGGLSIEETA
                     EVLKISHSTVERDWKLARAWLRRKLE"
     gene            1924..>3561
                     /locus_tag="VFH91_02010"
     CDS             1924..>3561
                     /locus_tag="VFH91_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6861783.1"
                     /translation="MTPERWEHVKEIFNSAIKYPHEERGVFLAQACSNDVALRNEVES
                     LIASHERSGEFIDEPAYQLAANSLVDERTELRPGQRIESYEIITFINRGGMGEVYLAQ
                     DKRLNRKVALKILPASFTRDTDRLRRFEQEARAASALNHPNIITIYEILQTDSNHVIV
                     TEFVEGETLRQRLSRTSLRLADAIDIASQIADALSAAHKAGIIHRDIKPENVMLRPDG
                     YIKVLDFGLAKLNEDVSQAVALEAPTMQVRTGSGIVIGTAGYMSPEQARGVTVDERSD
                     IFSLGTVMYEMVGRRKPFDGETPSDTLAAILKTEPPTLLQLDARVPAELNRIVTKSLR
                     KDREERYQVVKDLLLDLRALKQELDFQSKLERSAVPDHTDESTIHVSQAAATARSLQA
                     QDTESRSAINIITESLSLELKRHKVRSGVLLAGVVLILGLAGFGLYRFFNRPQPIEHF
                     WNIKLTRLTSSGNVIDATISPDGKYIVYVLSNRSTQSLWIRQVSTANDKQIVEPAPVG
                     YFGITFSPDGNDLYYALKTNLDSGTLYRIPVFGGVPVK"
BASE COUNT          915 a          850 c          937 g          859 t
ORIGIN      
        1 ccctgatggt tgtgtcggga ggcgtttggc aaatcccgct ttcagtcgat ggtgaaccag
       61 tgggtaaacc ggaacagatc gccaaccctg ctatggccca ggtgcgcaat ctcagctttt
      121 cagcagatgg aaagcgagtc gttagcagcg tcgtcagaca gaaaggtaac ctttggtccg
      181 ttcctatctc gaaaacttcc ggggaacgta tgggacaacc ggcgcctttc gtggaagata
      241 ccagctcaag aaagaccaat cccatttttt cgaatgacgg aagcaaggtg gcttacacag
      301 tttggatagc aggttttgcc ggcagtgttt ggacggttgg agaaaaaggt aacgatcgat
      361 tccaggtgac gactgatccg agctcaattg ttggttggat gccagaaggc aatcgcctcg
      421 tgagcatttc gtttcatgac gatgataact atttgacgac gaccgccgtg gataccggaa
      481 tgcgaaagac acttgcgaag ttgcaccggc accttccttt ttgcaggttg tcgccagacg
      541 gtaagacaat tgcgtttaat gcctacagcg atagcaatgt aaatctttgg tcgtattcac
      601 tcgaaagtga gacagaaaaa cagttgacgt tcgatcatga actttttggt tttcctgcct
      661 ggtctccgga tggcaaatgg atcgcaggtg agttgaaacg aaaagaggat acgtatgtgg
      721 cgatcgtgcc tgcggaaggt ggtgctccgg tgcaattaaa ttcggatcag ggccaaagtt
      781 ggaccgggag ttggtcgcca gacggtgaca agatcgcgtt tgcaggcttg aggaatggtg
      841 tatggaatat ttggtggatt tcggtttcga caagggagca gaaacaggtt actaactaca
      901 ccaaacccaa ttcattcgtt cgatacccga cctggtcgcc acgtggtgat caaattgtct
      961 acgaatattc agaactaacc ggaaacgtct ggctcgccga tttgaaatag gggccagagt
     1021 tcagccgtga agcccaacgc aaaacaagcg cacgtacaac gaaatagcaa ttctcaaatc
     1081 tcaaatgata attccccaat gatttctttc ccgacctctg acctctgatc cctgacccct
     1141 aattcctatt ccctggtgta gaatctttcc gttaaatcgt ttgaatccaa ccggagtgcc
     1201 ctcgtgcagc agaatgaagg cgttacccag ctcctggttg actggggcaa aggtgataaa
     1261 gaagcgcttg agcggttgat gccactcgtc tacagcgagc tccgccggct cgccacaaat
     1321 taccttcgcc gcgaacgtcc tggccacaca cttcagccca ctgccctggt caatgaagcc
     1381 tatcttcgac tcgtggacca aaaaaatgcg cagtggcaaa atcgcgccca attcttcggt
     1441 atctctgccc agttaatgcg tcgaattctg gtcgatcatg cccgtcagaa gcaggccgcc
     1501 aaaagaggtg ggagcggcca acagcgcttg tcaatcagta gtgcccacgc gtttgtcgat
     1561 ggacccggta tcgacttttt ggccctgaat gaagctctag aggagctgtc gaaaatggat
     1621 gcccagcaag aacggatcgt cgaactaaag ttctttggtg ggctgtctat agaggaaacc
     1681 gcagaagtcc tgaaaatttc acactcgacg gttgagagag actggaaact tgcccgggcc
     1741 tggctgcgtc gaaaactcga atgacctgag ggagtttgac cgaaaatttc gcgttataag
     1801 tggagccctg ttttgagagt acccggatgg tgagaaccgt aaccggcgac atttcataat
     1861 gaaatcgtca gccgaacgtt tgaagctagt aattccggat tatctttcga ataagagttg
     1921 gatatgaccc ccgagcgctg ggaacatgtt aaggagatct ttaattcggc gatcaagtat
     1981 ccccatgagg agcgaggcgt gttcctggcc caggcttgct caaacgacgt cgcgcttcgt
     2041 aatgaggtgg aatccttaat tgcctcgcac gaaagatccg gagagtttat tgacgagccg
     2101 gcctatcaat tagctgccaa ttccctggtt gatgagcgaa ctgaactcag accagggcag
     2161 cgcattgaat cctacgagat cattactttt attaatcgtg gcggaatggg agaagtttat
     2221 ctggcccagg acaagcggct gaacagaaaa gtcgccttga agatccttcc ggcatctttt
     2281 accagagaca ctgaccgctt aagacgtttt gaacaggagg caagagctgc ctctgcgctc
     2341 aatcatccca acatcattac gatttacgag attcttcaaa ctgactcgaa ccacgtgata
     2401 gtcacggagt ttgtcgaagg cgagactctg cgccagcgct tgtcacgaac ttctttacgt
     2461 cttgctgatg cgatcgacat cgcttcacaa attgctgacg ctctttctgc tgctcataaa
     2521 gcaggaatta ttcatcgaga catcaagccg gaaaacgtaa tgctgcgtcc agacggctac
     2581 atcaaggttc tcgatttcgg tctggcgaag ttgaatgaag atgtctcaca agcagtagcg
     2641 ctcgaagcgc ctacgatgca ggttcgaacg ggatcaggca tcgtaattgg tactgcaggt
     2701 tacatgtctc ccgaacaggc gagaggggtc acggtcgatg aacggtcaga tattttcagt
     2761 ttagggaccg taatgtatga gatggttggg cgtcgtaaac catttgacgg tgaaacaccg
     2821 agcgacacac tggcggctat tttaaagact gagccgccca cgttgttgca actcgacgcg
     2881 agggttcctg ccgagctgaa tcgtattgtc acaaagtcgc tgcgaaagga tagagaggag
     2941 cggtaccagg tagttaaaga cttgttgctg gatcttcgag cgctcaagca ggaacttgac
     3001 tttcaatcga agctggaacg ttccgcagta cctgatcaca ccgatgaatc aactattcac
     3061 gtgtcgcaag ctgcggctac cgcgcgaagc ttgcaagctc aagacacgga gagtcgtagt
     3121 gcaataaata taattacgga gtcgcttagc ctcgagctca agcgacacaa ggttcgcagt
     3181 ggtgtcttgc tggctggtgt tgtcttgatt ttgggtcttg ccggatttgg tctgtatagg
     3241 ttcttcaata gaccgcaacc aatagagcac ttctggaaca ttaagctcac tcgtcttacg
     3301 agttcaggaa acgttattga cgctaccatt tcgccagatg gcaaatacat cgtttacgtg
     3361 ctgagcaacc gtagtactca aagtctatgg attcgccagg tcagtaccgc aaatgataag
     3421 caaatagttg agccggcgcc ggtaggctac ttcggcataa ccttctcgcc ggatgggaat
     3481 gatctgtact acgccctgaa aacaaatctc gattcaggca cgttgtatcg cattcctgtc
     3541 tttggcggag tgccagtcaa g
//
LOCUS       DASSFP010000095         5149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_169614, whole genome shotgun sequence.
ACCESSION   DASSFP010000095 DASSFP010000000
VERSION     DASSFP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169614"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..3718
                     /gene="mfd"
                     /locus_tag="VFH91_02015"
     CDS             53..3718
                     /gene="mfd"
                     /locus_tag="VFH91_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET6861784.1"
                     /translation="MNSSTTSESILQSTLRRITNTPDYQRILQEIRNNARVISISGLV
                     AGSARALAVAALQRDSGRTFAIVAQSTRDLEPWERDLRFWYSALSGQESDEAQILVLP
                     ASETDPYAGISPHAQTLEKRAVALWRLQQHRPELLLLTARALARRTIRPAAIAKAGVV
                     LKRDQEHSPEVLVDQLISTGYMHEDPVKSIGEFSIRGGILDLWPPGQELPVRIEFFGD
                     TVDSLREFDPETQLSTGPIQKVDIPPMHELAVRADDFRFWAEVAKERWSDERYARSLH
                     DRTAFADEGEAFSGWEWLIPISINCDSNVFEHIGDAVLIIDEPAAIEGYLAEVYENLS
                     LRFNEIDQADDLALAPEELYLSIEELRQQIQNRQRVELRTLGRTATEVDQNLALEAEA
                     PKVQLGRARGPRLPTFLFPTAEAAMEVDWVAQSTMRYHGRVMDLAKGVARAAEDGRST
                     LFVMPSLGNAERVVEILAEYKVASHLALVEESSNFNENIPAIVTVGSLTGGFELPKAK
                     LIVHIESDVFDESAESVERRSAGPDTRKPGADSRKRRSKAAAFLSDFRDLRPDDFVVH
                     IDHGIARFGGLQTLDLGPRTGEFMLLFYADEAKLFVPVERMDLVQRYSSAEGHQPQLD
                     RLGGLGWQKTKAKAKRAMRDMADELLRLYAERKLVGGFAFAADTPWQKEFEDGFPFVL
                     TPDQETAIEDVKKDMQEAVPMDRLLCGDVGYGKTEVAMRAAFKSVMEGKQAAVLTPTT
                     VLAYQHYDTFRTRFAPFPVKVELLSRFRSPKEQKEVVKRVESGEVDVVIGTHRMLSKD
                     VQFKDLGLVVVDEEQRFGVAHKERLKQLKKRVDVLTLSATPIPRTLNMSLSGMRDMSL
                     IETAPRDRLAIQTHVVQFSDNVIKSAIELELSRGGQVFFIHNRVETIETIAGLVNRLV
                     PQARMAIGHGQMNEKDMEKVMLDFIDYKYDVLVATTIIENGIDIPRANTIIINRADQY
                     GLSQLYQLRGRVGRSSRRAYAYLLIPGEQELSPIARRRLAAIREFSDLGAGFRIAALD
                     LELRGAGSLLGGEQSGHLDALGFDLYTQMLERTVAELRGEAVEDETSVTLNLGVDVAI
                     PEDYISDMGQRLRTYKRVSSAQDENTLTSIRAETEDRYGRIPLSVERLFSYARLRKLA
                     EKVGILSVDRTPDGIAFKFSEKARVSPEKLAEFVGNHEGSSFTPSGVLRLVLDEEQQD
                     NVLEEARGVLLQLHATD"
     gene            3788..4900
                     /locus_tag="VFH91_02020"
     CDS             3788..4900
                     /locus_tag="VFH91_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6861785.1"
                     /translation="MRSRFITATGMAFMALLVFVSSGMPARAQEGEVTVVDEVIAQVN
                     DDVITLSMLKRETKERVEALVQRGMTEQQANEEVAKHQAELVATLVNEKLLLQKGKEL
                     ELASEIESEVNRRMLDIAREQGINSIQKLEEVMIQNKINPANVRETMRSEMMKQAVLQ
                     QEVDRRVYLGPSTAEVKKYYDAHPDKFRTPETVKLSEIYLSTNNKDEVSVKARATELV
                     TQIRAGADFVALAKANSEREKNGKRTAPEDGGDVGTFEVANLREDLLPAIKDVKVGGV
                     SDPIRTQEGYQILKVDARTAAGTTPTFNENKVREAMLFERQDKEREAYLQNLRNEAFI
                     KVSETYKDTVEPLLKLKAPVAAKGAEKEDTKKSKKP"
BASE COUNT         1307 a         1234 c         1423 g         1185 t
ORIGIN      
        1 ggtcccgatt gcttaaaggt tggattaatc agcacttata attttcgaaa gcatgaacag
       61 cagtacaaca tcggaaagca tacttcagtc aactcttcgg cgaatcacca acactcctga
      121 ctaccaacgc atactccaag agataaggaa taatgccaga gtcatctcaa taagtggtct
      181 tgtagcggga tctgctcggg ctctggcagt agcggccctc cagcgcgatt ccggaaggac
      241 gtttgccatc gtcgctcagt caactcgcga tctcgaacca tgggaacgtg atttacggtt
      301 ttggtactct gcactgtccg gacaggaaag cgacgaagca caaatccttg tattgccggc
      361 ttctgaaact gatccatacg cgggaatctc tcctcacgca caaactttgg aaaaacgcgc
      421 agtcgcgcta tggcgtttac agcagcatcg gccggagttg ttgctcctaa cggccagggc
      481 tctcgcgagg cgcactattc ggcctgccgc aatcgcaaag gcaggagttg ttctgaagcg
      541 cgatcaggag cactcgccgg aggtgctggt agaccagcta atctcaaccg gatacatgca
      601 tgaggacccg gttaagtcga tcggcgagtt ttctatcaga ggtggcatcc tcgacctttg
      661 gcctcccggg caggaacttc cggttcgaat agagttcttt ggtgacacgg tagattcgct
      721 tcgtgagttc gatccggaaa cccaactttc tactggccca atacagaaag tcgacattcc
      781 accaatgcat gaattggccg tcagggccga cgactttcga ttctgggctg aggtcgctaa
      841 agagcgttgg tctgatgaac gctatgcccg gtctcttcat gacagaactg cctttgctga
      901 tgaaggagaa gcattttcgg gttgggagtg gctgatacca atcagtatca attgcgactc
      961 taatgtcttc gagcatatcg gcgatgccgt attaattatt gatgagccag cagcgatcga
     1021 ggggtacctg gcggaggttt acgaaaacct ttcgcttcgg ttcaatgaaa tagaccaggc
     1081 cgatgatctc gctcttgctc ccgaagagtt atacctatcg atcgaagaac tgcgccaaca
     1141 aatccagaac agacaacgag tggaattgcg aacactgggc cgtacggcca cagaagtaga
     1201 ccagaatctg gccctcgagg ctgaagcacc taaagttcaa ttggggcgcg ccagaggacc
     1261 gcgtctacca acgttccttt tcccgactgc cgaggcggca atggaagtcg attgggtggc
     1321 ccaatcgact atgcgatatc acggtcgagt aatggacctg gcgaagggtg tggcgcgtgc
     1381 cgccgaggat ggtcgctcga ccctttttgt tatgccgagt ttgggcaacg ccgaacgcgt
     1441 tgtcgaaata cttgccgagt acaaagtcgc ttcgcatctg gctcttgtgg aagagagctc
     1501 taactttaat gagaacatcc ccgcgattgt aacggtagga agcttgaccg gtggtttcga
     1561 gcttcccaaa gcgaaactca ttgttcacat tgaatccgat gtcttcgacg agtctgccga
     1621 atcagtagaa agacggtccg cgggaccaga tactcggaaa ccaggagctg actctcggaa
     1681 acggcgttcg aaagctgcgg ccttcctatc cgactttaga gacttgcgac cggatgattt
     1741 cgtggttcat atcgatcatg gaatcgcgcg attcggcggg ttgcagaccc tcgatcttgg
     1801 gccacgcacc ggcgaattca tgctgctctt ttatgcagat gaagcaaagt tgtttgttcc
     1861 ggtcgaaaga atggacctgg tccagcgtta ctcgagtgct gaaggacatc aaccacaact
     1921 cgatcgcctt ggtggattgg gttggcagaa gacaaaagcc aaagctaagc gggccatgcg
     1981 cgacatggct gacgagcttt tgcgtttgta tgcggaaaga aaattggtgg gtggttttgc
     2041 attcgctgct gacacgccgt ggcagaaaga gttcgaggac ggttttccgt ttgtcctgac
     2101 tccggatcag gaaaccgcaa tcgaagatgt caaaaaagac atgcaagaag ccgtaccgat
     2161 ggaccgcttg ctttgtggcg atgttggcta cgggaagact gaggttgcga tgcgcgccgc
     2221 gtttaaatcg gtaatggaag gtaaacaagc ggctgtctta acgcccacaa ctgttcttgc
     2281 gtatcagcac tacgacacat ttcgaactcg ctttgctcca ttcccggtca aggttgagtt
     2341 gttatctcgg ttccgttcgc cgaaggaaca gaaagaggtg gttaagcgcg ttgaatctgg
     2401 cgaagtggat gttgtgatag gcacacatcg catgctttcg aaggacgttc aattcaagga
     2461 tctgggattg gttgtagttg acgaagagca gcgattcggc gtcgctcaca aggaaaggct
     2521 taagcagcta aagaagcgcg tcgacgtgtt gacgctttcg gcgacgccca ttccgagaac
     2581 gctgaacatg tcgctaagtg gaatgcgcga catgtcgctg atcgaaacgg ctccgcgcga
     2641 tcgtcttgct attcagactc acgtcgtgca gttttcggac aacgtcataa agtcagcaat
     2701 cgagttggag ctctcccgcg gaggacaggt ctttttcatt cacaatcgcg ttgaaaccat
     2761 tgaaacaatc gccggcctgg tgaatcgtct ggtaccgcaa gctcggatgg cgattggtca
     2821 cggtcaaatg aatgaaaaag acatggaaaa ggtcatgctc gactttatcg actacaaata
     2881 tgatgttctc gttgcaacga caatcattga aaacggaata gacatccccc gcgcgaacac
     2941 aatcatcata aatcgcgctg atcagtacgg tctctcacag ctttatcaat tacgaggacg
     3001 agtcggtcgc tcgagccgcc gagcttatgc gtatttgctc attcccggtg aacaagagct
     3061 gtctccgata gcgaggcgcc gattagctgc cattagggaa ttctcggatc tgggtgcagg
     3121 tttcagaatc gccgctcttg atctagagct gagaggggct ggaagcctcc ttggcggcga
     3181 gcagtcaggt catttggacg ctctaggttt tgacctttat acccagatgc ttgaacggac
     3241 agtggccgaa ttacgaggtg aagcggtcga agacgagacc agtgtgacgc tgaatctcgg
     3301 agtcgatgtt gcgatccctg aagattatat ttcggacatg ggccagcgtt tgcgcacgta
     3361 taagcgggtg tcatcagccc aggatgaaaa cactctgacg agtatccggg ccgagaccga
     3421 ggatcgatac gggaggatac cgctttcggt cgaacgtctc ttttcctacg cccgactgag
     3481 gaagctcgca gaaaaagtag gtattctttc agttgaccgg acaccggacg ggatcgcctt
     3541 taagtttagt gaaaaagcac gcgtttctcc cgaaaaactg gccgaatttg ttggaaatca
     3601 tgagggatcg tccttcactc caagtggcgt ccttcgcctt gttttggatg aagaacagca
     3661 ggacaacgtg ctcgaagagg ctcgtggcgt gttgctgcaa cttcatgcga ccgattagaa
     3721 ttcccgaaaa ttttggggtt cgtgaccgga ccctgacgca acaaccgatg tattaaggag
     3781 taatcttgtg agatccagat ttataacagc taccggaatg gccttcatgg cacttctcgt
     3841 ctttgtatct tccggaatgc cggcgagagc ccaggaaggt gaggtgaccg ttgtcgacga
     3901 agttatcgct caggttaacg acgacgtcat tacactttcg atgctcaagc gtgagaccaa
     3961 agagcgcgtc gaggctctgg ttcagagggg catgaccgaa cagcaggcca acgaggaagt
     4021 cgccaagcat caggcagagc ttgttgccac gctagtaaat gaaaagttgt tgttgcaaaa
     4081 aggtaaggag ctcgagctgg cgtccgaaat cgaatccgaa gtaaatcgtc gtatgcttga
     4141 catcgctcgc gagcagggaa ttaactcaat tcaaaagctc gaagaagtaa tgattcaaaa
     4201 caagatcaat ccggccaacg tccgtgagac gatgcggtcg gagatgatga aacaggcagt
     4261 gttgcagcaa gaagtcgacc gtcgtgtcta tcttggacca tcaaccgctg aggtcaagaa
     4321 atactatgac gcgcatcctg acaaattccg cacgcctgaa acagtcaagt tgtcagagat
     4381 ctatttgtca acaaataata aagatgaggt gagcgtgaaa gcccgtgcca ctgaactcgt
     4441 gacgcagatc agggcgggtg ctgattttgt tgcgttagcg aaagccaact ctgagcgcga
     4501 aaagaatgga aaacgcacgg cgcctgaaga cggaggtgat gttggcactt tcgaagtggc
     4561 aaatcttcga gaggatctgt taccggcgat caaggatgta aaggtcggag gcgtgtccga
     4621 tccgattcgt acgcaggagg gttaccagat tctcaaagtt gatgccagga cggccgccgg
     4681 aaccactcca actttcaacg agaacaaggt tcgcgaggcg atgttatttg agcgtcagga
     4741 taaggaaagg gaagcttatc ttcagaactt gcgtaatgag gctttcatta aagtgagtga
     4801 aacgtacaag gacactgtcg aaccgctgct gaagttgaaa gcccccgtcg ctgcaaaggg
     4861 agccgagaaa gaagatacca agaagagtaa gaagccatag gttgcgcctt tgtacttcgt
     4921 actttgcgct tcgttctttg tgctttgatt tttgtcgaac actcacaaca gaggacgaag
     4981 tgcaaagcac aaagcgctta gcccaaagct gacgcccatg cgtcttgatc ttttcctcaa
     5041 agccagtcgt ctttgccctc gacgtagcgt cgcccagaag ctgtgtgatg cgggtcaagt
     5101 tttcctaaat ggaacacagg tgaaatcggc acatgccgtg aaggcgggt
//
LOCUS       DASSFP010000096         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_170007, whole genome shotgun sequence.
ACCESSION   DASSFP010000096 DASSFP010000000
VERSION     DASSFP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170007"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFH91_02025"
     CDS             complement(<1..380)
                     /locus_tag="VFH91_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861786.1"
                     /translation="MMGNHFSIILLPTNKCNVNCEYCFEDKTDDRMSLDQLSTVIDKA
                     LDHMETADIGALTIHWQGGEIMTMPPEWFERAYDLIQNAADRRSKLVDHGLQSNMIGY
                     NSRWNPIIERMFDNSVGTSMDFPNV"
     gene            complement(377..1198)
                     /locus_tag="VFH91_02030"
     CDS             complement(377..1198)
                     /locus_tag="VFH91_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861787.1"
                     /translation="MGSLIYNIPASKIDLYRGYEVIIRSSNAAELAVSLSRCDPAQVR
                     FIQLLSTPADTSPLEGSFYSLPVDIHLTDPANEYIKLYDYASLLDSHPLRVSIEVAAG
                     FSKAVKLAVSLNFSVKLEADQPGPDLIAELASVLDLYLHRGSVRQPIEFFHSLLLSFC
                     RDEPASLWQIMEEDPSQVCYVTDDGEETISKRFIDQKELQPLRYAELFQRQGSSSMEC
                     YTCDFYDRCRGYFKWPDIEYDCAGVKKIFANLESSAKELQEDLAAYRAMEVEMQS"
     gene            complement(1249..1554)
                     /locus_tag="VFH91_02035"
     CDS             complement(1249..1554)
                     /locus_tag="VFH91_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET6861788.1"
                     /translation="MDQRLQKPDQTEEDRKLVTRRDFLAGLRKWSAAAIGVAVAGAVL
                     LPGETASGWVNSRGSWVNRGGGAWVNRATTWVNGGGGGGAWANRAGGGGAWVNRRGW"
     gene            complement(1569..2129)
                     /locus_tag="VFH91_02040"
     CDS             complement(1569..2129)
                     /locus_tag="VFH91_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6861789.1"
                     /translation="MFKRLIILCLATSLAFFIACAAGSTNREADTTASANSAAKPAAA
                     PVDQKVVVEEVRTVLQAHDKALSEKNIDALMETFSTDPNTVVLGTGTEERWVGPQEIK
                     AAYTEMFKDYDPNTLQAICDWKTGGSDPEGSMAWLAANCACKDSLQGKTRDYKLNVSA
                     SLAKQAGKWRFVVLHMSNAFQPPVTK"
     gene            complement(2429..2962)
                     /locus_tag="VFH91_02045"
     CDS             complement(2429..2962)
                     /locus_tag="VFH91_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6861790.1"
                     /translation="MSLNRVILFTGLILFASVTAQAQKVSVGYDHAIDFSKFKTYYWT
                     KGVPAKNPDIDKRIIEMVDQQMTLKGLTRAGVSGDIAISYHAAVMDTFDSATVARPGT
                     WGPSAGSVEAAWRVVKGALIIEMKDSTNTSEVWRATATDTISNEPLKDPAKDIEKVNK
                     KVQKVVEKIFKYYPPKK"
     gene            complement(3115..>4661)
                     /locus_tag="VFH91_02050"
     CDS             complement(3115..>4661)
                     /locus_tag="VFH91_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET6861791.1"
                     /translation="PPVVGIYSCILPLVAYALLGSSRQLVVNPDAAACSIVAATVGPL
                     AVGNPDRYLELSIGLTCFTGILLMIGGFSGLGAIANFLSRPILTGYLNGIALSIIAGQ
                     LGSLLGYKLSPNGFFRTVIEAVSRLKETQTSTFIVGLSLLVLLLLIKRFLPRLPAPLI
                     AAILGCAAVYALDLGAKGVAIVGAVPAGFPAPKVPSFSASEIGPLALGAAGIALVSFC
                     SMMTTARGFAAKNGYRIDVNRDMFALGVSDLASAVSQGFVVSGADSRTAVADSAGGKT
                     QMTSIAAAVVMAVVLLFFTKPLAYVPIAALAAILMSSALGLFDIASLRRYYRISVPEF
                     RQSVVATLGVMTVGVLPGVLIAVGLALLKLLRQVSRPRDSILGLVSGVDGDDFRSKEE
                     GGTSIPGIVIYRFEASIVFFNADYFIERVRTIIKAAGTRPKYFLIDAESVPVLDVSGA
                     YALEALHRELSQQGIGLGIARAPYLMRMMLERAGLNETIGTHNLYQTIHAGTRSFRED
                     GRSQSG"
BASE COUNT         1173 a         1243 c         1131 g         1114 t
ORIGIN      
        1 acatttggaa agtccatcga agtgccaaca ctattgtcga acattcgctc aatgatcgga
       61 ttccaacgag agttgtagcc gatcatgttt gactgcaacc cgtgatcaac caacttggag
      121 cgacggtcag cggcattctg gatcaaatca tatgctcgtt cgaaccactc gggcggcatc
      181 gtcattattt cgcctccctg ccagtggata gtgagtgcac ctatgtctgc tgtctccata
      241 tggtcgagag ctttgtcgat aaccgtggac aactggtcga gactcattcg atcgtccgtc
      301 ttgtcttcaa agcaatactc gcagttgacg ttacatttat ttgttgggag tagaatgatc
      361 gaaaagtgat tacccatcat gattgcatct ctacctccat cgcgcgatac gctgcgagat
      421 cctcttgcaa ctctttcgct gacgactcaa ggttcgcaaa aatcttcttt acgccggcgc
      481 agtcgtattc aatgtcgggc catttaaagt aaccccgaca ccgatcataa aagtcgcagg
      541 tataacattc catcgaactc gatccttgtc gctggaaaag ctcagcgtat cgaagaggct
      601 gcaactcttt ctgatctata aagcgctttg agatcgtctc ttcgccatca tcggtgacgt
      661 aacagacttg ggatgggtct tcttccataa tttgccaaag agaggctggc tcgtcgcgac
      721 aaaacgaaag taagagactg tgaaagaact caattggttg acgaacgctt ccccggtgta
      781 gatacaggtc gagaacggaa gccagttctg cgattaggtc gggacccggc tggtccgctt
      841 ctaatttaac tgagaaattc agcgagactg cgagtttaac tgccttacta aaacccgctg
      901 caacttcaat tgaaactcga agtggatgag aatcgagaag acttgcgtag tcgtagagtt
      961 ttatgtactc gtttgccgga tcggtgagat gtatgtcgac tggcaacgaa tagaacgagc
     1021 cttcgagtgg gcttgtatcc gccggcgtcg aaagcagttg gataaatcgc acttgcgcag
     1081 gatcgcaacg tgaaagagat accgcaagtt cggcagcatt cgacgacctt atgatgacct
     1141 cataaccgcg atacagatcg atcttgctgg caggaatgtt gtaaatgagt gagcccatca
     1201 gagtagacct tcactcccgg tctttagacg tgtttctaaa tccgactatt accaaccgcg
     1261 acgattgacc cacgcaccgc cgccacctgc gcgattggcc caggcgcctc caccgccgcc
     1321 gccatttacc caggttgtag cgcgattcac ccaggcacca ccgccacggt taacccagga
     1381 gccacgggaa ttcacccagc cagaagctgt ttcgcccggc aataaaactg cgccggcgac
     1441 cgccactccg atcgctgctg ccgaccattt tctcagccca gcaaggaagt cgcgccgcgt
     1501 aacgagtttg cgatcttctt ctgtctgatc cggtttttga agtctctggt ccatggtcca
     1561 cctccctgct actttgtcac cggcggttga aaagcattgg acatgtgcag cactacaaaa
     1621 cgccacttgc cggcctgctt tgccagactt gcagacacgt tcaacttgta atctcgagtc
     1681 ttgccctgca gcgaatcctt gcacgcgcag tttgcggcga gccacgccat tgatccttca
     1741 gggtcggaac cacccgtttt ccagtcgcaa attgcttgca gcgtattcgg atcgtagtcc
     1801 ttgaacattt ctgtatacgc tgccttgatt tcctgtggtc caacccatct ttcttctgtc
     1861 ccggtgccca ggacaaccgt gtttggatca gttgagaacg tctccatgag agcgtcgata
     1921 ttcttctcgc tgagtgcctt gtcatgcgct tgtaggacgg tacgtacttc ttcgactacg
     1981 actttttgat caacaggcgc ggcggcgggt ttggccgcag agtttgctga ggcggtggta
     2041 tcagcttcgc ggttggtgct cccggcagcg cacgcaatga agaaggctaa cgaagttgcc
     2101 agacacaaaa tgatcaatcg tttaaacacg actattcctc cttcttttgg tccgaatatc
     2161 gggggcagtt acttcgtgag tacccgtcga catcgagagg ctcatttcgc aacagcgggc
     2221 gctcacggaa tggaaaccct acaattttgc ggagaattcc aagacccagc ttagaaaaaa
     2281 cccttaaatt ggataatcag gtgaagtctg taaatcgcgg cgggatttgt ggtagttagt
     2341 taattgtccg tagtccgttg tcccagatcg tcgttgggat aggaccttta tgacctatag
     2401 gacgtattcc agaaacaaac tgacacaatc actttttcgg cgggtagtat ttaaaaatct
     2461 tttcgacgac cttttgcacc ttcttattga ccttttcaat atcctttgcg ggatccttca
     2521 gcggttcgtt gctgattgta tcggtggccg ttgctcgcca cacttccgat gtgttcgtgc
     2581 tgtccttcat ctcaattatt agcgcaccct tcaccacccg ccatgctgcc tcgacacttc
     2641 cggcgctcgg tccccaggta cctgggcgcg caactgttgc ggaatcaaac gtgtccatta
     2701 cagcggcgtg gtacgagatg gcaatgtctc cactgacacc ggcgcgtgtc agtcctttta
     2761 aagtcatctg ctgatcgacc atttcgataa tccgtttgtc gatgtcggga ttcttcgcag
     2821 gaacaccttt tgtccagtaa taagttttaa actttgagaa gtcgatggcg tgatcgtatc
     2881 caacggacac cttctgggcc tgtgcggtga ctgaagcaaa cagaataaga cccgtaaaga
     2941 gaattacgcg attaagtgac atctgagatc ctccggcacc actaaacaaa ttaattacag
     3001 gcttcgacca tctttgtatc atcgaaacag tttcgcaaca agatcagttg tttaagggac
     3061 acacctaatg ggagtacgct gctaacgtcc gaattttttg agtcgttgga atatctaacc
     3121 agattgagaa cgtccgtctt cgcgaaagct cctcgttccg gcatgaattg tttgatagag
     3181 attatgcgtt ccgatcgttt cgttgaggcc ggctcgctca agcatcatgc gcatcaaata
     3241 aggagcacgg gcaatgccaa gcccaattcc ctgctggctc aattctcgat gtaatgcttc
     3301 gagtgcataa gcgcccgata cgtccagtac tggtaccgat tcagcatcga tcaagaaata
     3361 tttcggtcga gttccagccg ctttgatgat tgttctaacg cgctcgataa aatagtcagc
     3421 attgaagaaa actatcgacg cttcaaagcg gtaaatcact attccgggaa tacttgttcc
     3481 tccttcttct ttcgaccgaa aatcatctcc atctacaccg ctgaccagtc caaggatcga
     3541 gtctctggga cgcgatacct gccttaataa tttcaacaac gcgaggccca cagctatcag
     3601 gactcccggc aagacgccta ccgtcatcac tccaagagtg gcaacgacag actgccggaa
     3661 ctcgggcacg ctgatccggt aataacggcg aagtgatgcg atatcaaaga gacccagagc
     3721 agaagacatc agtatcgcag cgagcgccgc aattggaacg taggccagcg gttttgtaaa
     3781 aaacagcaag acaacagcca tgaccacagc agcagcgatg gaagtcattt gagtttttcc
     3841 gccagccgag tctgcgaccg cagtgcgaga gtctgctccg ctaactacaa agccctggct
     3901 aacggcggac gccaaatcag aaacgcccag ggcaaacatg tcgcgattaa catcaatgcg
     3961 atagccgttt tttgcggcga atccacgtgc agtcgtcatc atgctgcaaa aactaaccag
     4021 agcaattccc gccgcgccca gcgccagtgg tccaatttct gatgcactga aactcgggac
     4081 cttcggcgcc gggaatcccg ccggcacagc gccgacaatc gcaacacctt tcgctccaag
     4141 atcgagtgcg taaactgctg cgcaacccaa tatcgcagca atcagcggag ccggcagcct
     4201 cggaagaaat cgtttgatca gaagtaggag gaccagcaac gacagaccaa caatgaaagt
     4261 cgacgtctgt gtctctttca aacgcgagac tgcttcgatc acagttcgga aaaaaccatt
     4321 cggcgaaagt ttgtaaccga gcagggagcc cagttggccc gcgataatcg acaatgcaat
     4381 tccgtttaaa tagccggtaa gaatcggtct cgaaagaaag tttgcgatcg cgcctaaacc
     4441 cgaaaaacca ccaatcatta ggagaattcc agtgaagcaa gtcaacccaa tcgacagttc
     4501 gagataccgg tcggggtttc ccactgcgag tggaccaacc gtggcagcca cgattgaaca
     4561 tgcagcggca tcagggttga ccacaagttg acgtgaggaa cccagaagag cgtaagccac
     4621 aagtggcagg atgcaggaat aaatgccaac cacaggcggg a
//
LOCUS       DASSFP010000097         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_467469, whole genome shotgun sequence.
ACCESSION   DASSFP010000097 DASSFP010000000
VERSION     DASSFP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467469"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1193
                     /locus_tag="VFH91_02055"
     CDS             99..1193
                     /locus_tag="VFH91_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861792.1"
                     /translation="MAELFENVEVNRDPRWPILIRLLGGSLVLHLLLLWLVVYVPAFR
                     DALNVASLIASTRIVDKDYRATHIGDDVELLDLADEKFHYPDGYFALEQSFETGQSQP
                     GSNFDAFAPKIISLAQPPGKDEPDLSPSPSPSPVPSPTPSSSPPSAVAQETLPNAQGS
                     PSPLTKDEAQQKLEDTAAENNIDLPTENQLNKKVLRDFAIYANELKKQGKLDLDKPFE
                     IVIEAQLDESGKLKDPRFTKKAGDANLIDLFGRMVAALNDSGFLIYLKPLNKDNPGAT
                     VKFTIKQGENEVLASVESEASSTDSARILAKALNAALVFGAGSRAGKDEEVIMRNTNA
                     SPDGKKIVVNFSMPRQSVVDLIKKQLEPGV"
     gene            1310..3856
                     /gene="secA"
                     /locus_tag="VFH91_02060"
                     /pseudo
     CDS             1310..3856
                     /gene="secA"
                     /locus_tag="VFH91_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            3911..4090
                     /locus_tag="VFH91_02065"
     CDS             3911..4090
                     /locus_tag="VFH91_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET6861793.1"
                     /translation="MARRPASLTFTGPNQGAAPAGEEEGKIKTVVRDQPKVGRNEPCP
                     CGSGKKYKKCHGATA"
     gene            4211..>4780
                     /locus_tag="VFH91_02070"
     CDS             4211..>4780
                     /locus_tag="VFH91_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861794.1"
                     /translation="MSTNPVVQAIIGGTAPRPARLAAARGMLPLPQNDRLEILISLQA
                     TDDQELSAAASETLDAESPEDLLMVAKGEDSPLTVLSYLATRTESSRELHEAIVLNSQ
                     TPDEALAKLASTTTEGSLLELVAVNQQRLVRFPALIEAILGNPARTPEAERRAKETKV
                     EFFEKERGARQIADELRARGQTAAAEFFEE"
BASE COUNT         1287 a         1140 c         1296 g         1057 t
ORIGIN      
        1 ggggttacaa tctgcttcac aacgcgactg atgattgacg cgtctaaatg cagtgaaacc
       61 tgcgaaaagt tctattcgtt tacggagcgg gtgtagtaat ggccgagttg ttcgaaaacg
      121 ttgaggttaa tcgcgatcct cgctggccga ttctaattcg gcttcttggc gggtccctgg
      181 ttctgcattt gctgcttctt tggctggtag tttatgtgcc tgcgtttcgg gatgcgttaa
      241 atgtggcgag cctgatcgcg agcacccgaa ttgttgataa agactatcgg gccacacata
      301 tcggcgatga cgttgaactc ttagatttag cagacgagaa atttcattat ccggatgggt
      361 atttcgccct cgagcagtcg tttgagacag gacagtcgca acctggttct aattttgacg
      421 cgtttgcgcc aaagataatt tcgctcgctc agcctccggg aaaggatgaa cccgacttaa
      481 gtccgtctcc gagtccgtcg ccagtaccca gtccgactcc atcaagttca cctccgagtg
      541 cggtcgcgca ggagacattg ccaaacgctc aaggcagccc ttcgccatta acaaaagacg
      601 aagcccagca gaaacttgag gacacagcgg cggagaacaa tatcgatcta ccaactgaga
      661 atcagctcaa caagaaagta ctgagggact ttgctattta tgcaaatgag ttgaagaagc
      721 aagggaagct cgatctcgat aagcccttcg aaattgtgat cgaggctcag ctggacgaat
      781 caggcaaact gaaggatccg cgcttcacga agaaagcagg ggatgcaaac ctcattgatc
      841 tatttggccg catggtggca gcgctcaatg acagtggttt tctcatttat ctgaaacctc
      901 tgaacaagga caatccgggt gctacggtta agttcacgat caagcagggc gagaatgaag
      961 tgcttgcgtc ggttgagtcc gaggcctcat cgacagacag tgcgcggatt ttggcaaagg
     1021 ctctgaatgc ggcgcttgtt ttcggagccg gctcaagagc tggcaaggat gaagaggtga
     1081 tcatgagaaa cactaacgcc tcgcctgacg ggaaaaagat tgtggttaat ttttcaatgc
     1141 cgagacagag cgtagtggat ctgatcaaga agcagttgga gcccggtgtt taaactgact
     1201 ggcaaagtag cttacttgcc tcacttacat tgctaaacta aaattaaacg ttgtcaatga
     1261 gttagatatt ttttagttca ggacagtaga ccggagcaaa ttattagata tgaatttcga
     1321 taaggcttta acaaagatat ttggcagcag taatcagcga ttcctgaaaa cgattcaacc
     1381 gctagtcgag cagatcaact cgctcgagcc tgcaatgaaa cagctctccg acgacgaatt
     1441 gcgcgccaaa accgcagtct tcaaagaaca agtccggaga gtcgttggag atacgaaaga
     1501 caaagaagag aggaaaattc gcgaacgaca agcgctcgac gagttgttgc ctgaagcttt
     1561 tgcgacagtg cgcgaagcca gcaggcgcac caccggcatg cggcactttg acgtccagat
     1621 gatcggcgga attgtgttgc accagggcaa gattgccgag atgcgcacag gtgaaggtaa
     1681 aactcttgtc gccacgcttc catcttatct gaacgccttg actggacgag gcggcgtcca
     1741 cgtcgtgacc gtcaacgact atctcgcttc tcgcgacgct gaatggatgg gccagattca
     1801 tcgatttctt ggcctcgaag ttggctgcat tcaaaacgac atggatgatt ttgaacggca
     1861 ggcagcatac gcggcggaca ttacctacgg taccaacaac gagtttgggt ttgactatct
     1921 gcgcgacaac atgaagttcg atctggctac ttgcgtgcaa cgcggccact acttcgctgt
     1981 tgtggacgaa gttgactcga ttctgatcga cgaggctcga accccgctca tcatttctgg
     2041 cgcatctgac gaagcgactg ataaataccg gaatgccgac gcaattattc ctcacctcaa
     2101 aaagggcgag gatgtcgacg gcaagaaaac cggagagtat gtggtcgatg aaaaggccca
     2161 cacagctgtt cttaccgaag aaggcgtcga gagagccgag aaactacttg gtgtcgggaa
     2221 cctctatgat ccttcgaata tggagttgct gcactgtgtc gaacaggcgt tgaaggcgca
     2281 tacgctttat aaactggacc accaatatgt ggtccaggat ggcgaagtca tcatcgtcga
     2341 cgacttcacc ggacgtttga tgaaaggccg gcgttggtcc gacggtctgc accaggcagt
     2401 cgaggcgaaa gaaggcgtcc aaattgagag agagaatcaa acgctcgcta ccatcacgct
     2461 gcagaactat ttccggttgt atgaaaaact atcggggatg accggtacag ctgagacaga
     2521 agcggcagag tttgcctcga catataagct cgacgttatc gtgattccca ctcacatgcc
     2581 gatggttcgt tcggatttct cggatgtgat ctacagaact cttccggaaa aatgggacgc
     2641 ggtgatcgaa gagataaaag agtgttacga gaagggccaa cctacgcttg ttggtaccgt
     2701 ctcggtggag aactcagaac taattgccag aaggttggtg aaagagtcgg tgccgcacaa
     2761 tgttttaaat gcgaagtatc acgagcgcga agccgagatt gtggctcagg ctggtcgaaa
     2821 aggctcggtg acgatcgcta cgaacatggc tggtcgcgga accgacattc tgctgggcgg
     2881 caatcccgac tttatggctc gcgagtttct aaaggaggaa gagatcgatc ctgaagaagc
     2941 ttccgaacag cagtgggaac agatgtttgc gcgcgctaaa cggatagtcg aagaagaaca
     3001 caaagaggtg gtcagccttg gtggcttaca catcctcggc actgagcgtc acgagtccag
     3061 gcggattgat aatcagctaa gaggtcgtgc cggacgtcag ggcgaccccg gatcttcaag
     3121 atttttcctt tcacttgaag acgatctgat gcgcatcttc gcgggcgaca aggtcaaggc
     3181 gttgatgcag cggctgggga tggaaaaagg cgtggccata gagtcgaaga tggtgtcgaa
     3241 gcggatcgcg gcggctcaaa aatcggttga aggtcgtaac ttcgaatccc gcaaacacgt
     3301 ccttgaatat gacgacgtaa tgaacaagca gcgcgaaacg atctatggat tgcgccggca
     3361 gttgatggaa gagcccgatc agcgagatta cttgttgggc gatccgccgg cagctggagt
     3421 cgcttaagac ctgctgtctg atttgaccaa acagtatctg aatcccgacg tttcacctga
     3481 cgactgggat atcgacaact acaagattca ggtcaaaacg atttatgacc tcgacagcga
     3541 ttccgaaggc atcaacatcg ccgcaatgac ctcaccggaa gtcactgatg ctatctggga
     3601 aaagcttaag gttaagtacg cgttcaaaga ggaacagatt ggaccagaag cgatgcgcac
     3661 ttacgagcgc atcatcatgc tcaacattat cgatgcgcag tggaaagatc acttattgtc
     3721 gctcgaccac ttgaagcagg gtatcgggtt ggtagggtat ggacaaaagg acccgctcgt
     3781 tgaatacaag aaacagagct tcgatctttt tcaggatatg ttggatcgca tcgacaccgc
     3841 tacgattcga tcgctcttta atcttcaagt agtggcggaa caaccgcctg agacgttgca
     3901 acaacgtcgg atggctcgcc gtcccgcttc tctcacgttc accggtccaa atcaaggcgc
     3961 agctccggca ggtgaagaag agggcaagat aaagacagtc gtcagggatc aacccaaagt
     4021 tggtcggaat gaaccatgtc catgtggctc aggcaaaaaa tacaaaaagt gtcatggagc
     4081 tactgcttga atccgcagat tacaccgatt gattccaaac accgtatcgc gatcgttgaa
     4141 aagctggatc ataaaatcga tgattggggc tttcatcgaa tctgtggaat cggtgtaatc
     4201 ggtggataaa ttgtctacaa atcctgtagt ccaggcaatc atcggtggga ctgctccgcg
     4261 accggcacgc ttagcggcag cgcgaggcat gctaccgctt ccgcaaaatg atcgacttga
     4321 aatactgatc tcactgcaag caaccgatga tcaggaatta tcagcggctg cgtcggaaac
     4381 actcgacgcc gagtcgccgg aagatcttct gatggtcgcc aaaggagaag actcacccct
     4441 tactgtgctc tcctatttgg ccactcggac ggaatcatca agagagctcc atgaagccat
     4501 cgttttaaac tcacagactc cagatgaagc ccttgcgaaa ctcgcttcga ctacaactga
     4561 aggatcattg cttgagctgg ttgcagtaaa tcagcagcga ctcgttcgat tcccggcact
     4621 gatcgaggcg atcctgggaa atccagcacg tactcctgaa gccgaacgcc gtgcgaaaga
     4681 aaccaaagtc gaattctttg aaaaggagcg aggcgcacga caaatcgctg acgaactccg
     4741 ggcccgagga caaactgccg cggccgagtt ctttgaggaa
//
LOCUS       DASSFP010000098         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1213144, whole genome shotgun sequence.
ACCESSION   DASSFP010000098 DASSFP010000000
VERSION     DASSFP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213144"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /gene="cutA"
                     /locus_tag="VFH91_02075"
     CDS             complement(<1..332)
                     /gene="cutA"
                     /locus_tag="VFH91_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET6861795.1"
                     /translation="MKDEVQTDLDPIVVFITAANGEEAARLADLLVGANLAACVQILP
                     EIESVYRWKGKIERQSEILLFAKTSRAKFSELEREVRNLHGYETPEIVAVPIVEGSKA
                     YLEWLDETL"
     gene            complement(322..699)
                     /locus_tag="VFH91_02080"
     CDS             complement(322..699)
                     /locus_tag="VFH91_02080"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET6861796.1"
                     /translation="MFCPVCESEYEPGIARCPDDNTPLVDKLTSENTVHDNSEARYLV
                     LHKLGQPAEAEMVNEILQNNGIRSVVKAGGSDALSPLLSATSEGAVILVDERDFDRAQ
                     EIYNAFFGRDTTPLTGGSTDDEG"
     gene            complement(733..1680)
                     /locus_tag="VFH91_02085"
     CDS             complement(733..1680)
                     /locus_tag="VFH91_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6861797.1"
                     /translation="MSLLVVGSVAFDAVETPFGKCEQMLGGSASHFSISASFFTDVSI
                     VGVVGGDFTEEDQRVFVERNIGTGDLERVPDGKTFRWRGRYEYDLNVAHTLETQLNVF
                     ADFKPKLSDESKSSRLLFLGNIQPDLQREVREQMPNAELVALDTMNLWIENTRDSLLR
                     TIRGVDVVIINDAEARQLTNIPNLIKAAREILSWGPKALIVKRGEYGAALFTADSYFA
                     IPAYPLESVFDPTGAGDTFAGGFMGYLSSQEKLDEATMRRAMIFGSVMASFNVEEFGT
                     ERVRRLTYSEINERFRSFKQMTNFEEIPFERASQLTSSR"
     gene            complement(1723..2217)
                     /gene="bamD"
                     /locus_tag="VFH91_02090"
     CDS             complement(1723..2217)
                     /gene="bamD"
                     /locus_tag="VFH91_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET6861798.1"
                     /translation="MWTKFLLGLVAVMILGTASTAVRGQGTVRSGPDPATIRDPELEK
                     DSLHNFEVAQNYFKLKKAYVAALKRLEEIIAGNPNFTRIEEVLLMAGQSSLWLADGKG
                     KQKSDKYVTFDGGVKHTLTPEQFREMGRGYLTKLINDYPNSPFAKEAQEELRLLGTTV
                     SAKP"
     gene            complement(2235..3122)
                     /locus_tag="VFH91_02095"
     CDS             complement(2235..3122)
                     /locus_tag="VFH91_02095"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET6861799.1"
                     /translation="MSLPTVKIGIIGGSGLYQMSELTDIEEIKVDTPFGDPSDAFILG
                     SLEGERVAFLPRHGRGHRFTPTEVPFRANIYAMKLLGVERILSASAVGSLQEQYAPLD
                     MVIPDQFFDRTRARAKESTFFGEGIVAHVSFAHPVCRALGDVLQQSCETVSVKTHRGG
                     TYLCMEGPAFSTVAESNVYRSWGMDIIGMTNLQEAKLAREAEMCYATLALVTDYDCWH
                     PSHDAVSVEAVIEYLNKNVRNAQLIMRESVKVLKSSERSCKCGSALKNAIFTAPALWP
                     TSTTKKLEAIIKKYDSSAN"
     gene            complement(3236..>3597)
                     /locus_tag="VFH91_02100"
     CDS             complement(3236..>3597)
                     /locus_tag="VFH91_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861800.1"
                     /translation="PLQKTGQLEVKDDVYEVVPGLQMELQPGHNRSMQCWRLRQGGKT
                     MFGFADLVPMRAHVRFPWIMGYDLYPVETLEAKKKLLPQAARENWLCLFYHDPDEPLC
                     TLEEHEGKLVSRPVNDV"
BASE COUNT          877 a          963 c          824 g          933 t
ORIGIN      
        1 agtgtctcgt caagccactc cagataagcc ttcgatcctt caactatcgg aaccgcaacg
       61 atctccggtg tttcatagcc gtgaagattg cgaacctctc gttcgagttc tgagaacttc
      121 gctctggatg ttttcgcgaa cagaagaatt tctgactgtc tctcgatctt acctttccat
      181 cggtagaccg attcaatttc aggcaaaatc tgtacacagg ctgcgaggtt tgcaccaacc
      241 agaagatctg cgagacgagc ggcctcttcc ccgttcgccg ctgttatgaa aacgacgatc
      301 ggatccagat cagtttggac ttcatccttc atcatcggtt gaaccaccag taagcggtgt
      361 tgtatcacga ccaaagaacg cgttgtatat ttcctgtgcg cgatcaaagt cgcgttcatc
      421 aaccaggatg actgcgcctt cagaagttgc cgacaacaac ggcgacagag cgtctgagcc
      481 tccagccttt acaacggaac gaattccatt gttctgcagt atttcattta ccatttcagc
      541 ttcagccggc tgcccgagtt tatgcagcac caaatagcga gcttcgctat tgtcgtgaac
      601 tgtattctcg gacgtcagtt tatcgaccaa cggggtgtta tcgtcggggc accgggcgat
      661 gccgggctcg tattccgatt cacaaacggg acaaaacata gcggtttttc tccggcctga
      721 ctatttgatg cctcaacgac tagatgtcaa ttgcgatgct ctttcaaagg ggatctcttc
      781 gaagttcgtc atctgtttaa aagagcggaa tctctcatta atttcggaat acgtaaggcg
      841 tcgaactctt tcggtgccaa actcttcgac gttaaaagaa gccatcaccg atccgaaaat
      901 catcgcgcgc ctcatggttg cctcgtccaa tttttcctga ctggaaagat aacccatgaa
      961 gccgcctgcg aacgtgtcac cagcgccagt tggatcgaat actgactcca acggatacgc
     1021 tggaatcgca aaatagctgt ccgcggtgaa tagcgccgcc ccgtattcgc ctcgtttgac
     1081 gatcaacgcc ttcggtcccc acgaaagaat ttcgcgcgcg gctttaatca agtttgggat
     1141 attcgtcaac tggcgcgcct ctgcatcgtt gatgatcaca acatcgacgc ctcttatcgt
     1201 ccgaagcagc gaatcacgcg tattctcgat ccacaaattc attgtgtcga gtgcaaccaa
     1261 ttctgcgttg ggcatctgct ctcgaacttc ccgctgcaaa tccggttgaa tgttgcctag
     1321 aaagagcaaa cgcgaagatt tcgattcatc agacagcttg ggcttgaaat ctgcgaatac
     1381 gtttagctgg gtttccagcg tgtgcgcaac gttcagatcg tattcgtagc ggccacgcca
     1441 acgaaatgtt ttaccgtcgg gaacacgttc gagatcgccc gtaccaatgt tgcgttcgac
     1501 aaaaaccctt tggtcctctt cagtaaaatc gccgcccact actccaacga tgctaacgtc
     1561 tgtaaaaaag cttgccgaga tcgaaaagtg cgaagccgaa ccaccaagca tctgttcgca
     1621 ttttccaaat ggagtttcga ctgcgtcgaa tgcgacagaa cccaccacaa ggagagacat
     1681 ttgtttatca accttggact cacaaaatag taaagcgtta gattaaggct tagccgacac
     1741 agtagttccg agtagccgca attcttcctg ggcttctttt gcgaatgggc tattcggata
     1801 atcgttaatc aatttcgtga gataaccgcg tcccatctcg cgaaactgtt ccggcgttaa
     1861 tgtgtgtttc actccaccat caaaagtgac gtacttatcg gacttttgct tgcccttgcc
     1921 gtcggccagc cacaggctgc tttgtccagc catcaacaac acttcttcaa ttcttgtgaa
     1981 gttgggatta ccggcaatta tctcttcaag acgtttcagc gccgcaacgt aagccttttt
     2041 tagcttgaaa tagttttgtg ctacctcgaa gttatgcaac gagtctttct caagttcggg
     2101 atcccgtatc gtggcaggat ctgggccaga acgaacagtt ccctgccctc gaacggccgt
     2161 actggcagta ccgagaatca taaccgccac caggccgagt aaaaatttag tccacatatt
     2221 ccacctcacc ccactcagtt agctgaagaa tcgtactttt tgatgatcgc ttccagcttt
     2281 tttgttgtcg acgtgggcca aagagcagga gcagtaaaaa tcgcgttttt cagcgccgac
     2341 ccgcatttac agctccgttc agaactcttc aataccttga ccgattcccg catgattaat
     2401 tgcgcattcc gaacgttctt gttcaggtac tcgatgaccg cctcaacact tacagcatcg
     2461 tgcgaggggt gccaacaatc ataatcggtg actaacgcca gcgttgcata gcacatctct
     2521 gcttctcggg ccaacttcgc ttcctgaaga ttggtcattc caatgatgtc cataccccag
     2581 gaacgataga cattcgattc tgccacggtt gaaaacgccg ggccctccat gcacaggtag
     2641 gttccaccgc ggtgagtctt caccgaaacc gtctcgcaag actgctgcag aacgtctccg
     2701 agagccctgc aaacgggatg agcgaatgaa acgtgggcca cgatgccttc gccaaagaaa
     2761 gtcgattctt ttgcgcgcgc gcgtgttcgg tcaaaaaact gatctggaat gaccatgtcc
     2821 aacggagcat actgttcttg tagagaacct actgctgagg ccgacaaaat tcgctcgaca
     2881 ccgagcaatt tcattgcgta gatgtttgct cgaaaaggca cctcggtagg tgtgaaccga
     2941 tgaccgcgtc catgtcgagg caagaaagct acccgttcac cttcaaggct tcccaatatg
     3001 aatgcatctg aggggtcacc gaatggtgta tcgactttga tttcttcgat gtctgtcaat
     3061 tcagacattt gatagaggcc gctgcctccg atgatgccga tttttactgt tggaagagac
     3121 attgttgggt gtcagttgtc tgtgctttgt gctttgtttt ttgtactttg tactttgcgc
     3181 tttgtacttt gtgctaagag caaactacaa agagcaaagt gcaaagcaca aaccatcaca
     3241 cgtcattaac aggccgcgat accagtttcc cctcatgttc ctcgagcgtg catagtggct
     3301 cgtcgggatc gtgataaaac aggcaaagcc aattttcgcg tgcagcctgc ggcaataatt
     3361 tcttcttggc ctcgagagtc tccaccggat ataggtcata tcccattatc cagggaaaac
     3421 gtacatgggc acgcatgggt acaagatcag cgaaaccaaa catcgttttg ccgccctgtc
     3481 gtaagcgcca gcattgcatg ctgcgattgt gaccgggctg caactccatt tgcagtcccg
     3541 gaactacttc gtaaacatcg tccttaactt ccaattggcc cgttttctgc aacggct
//
LOCUS       DASSFP010000099         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1064463, whole genome shotgun sequence.
ACCESSION   DASSFP010000099 DASSFP010000000
VERSION     DASSFP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064463"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1680
                     /locus_tag="VFH91_02105"
     CDS             496..1680
                     /locus_tag="VFH91_02105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET6861801.1"
                     /translation="MKKISALIVLACFCAVMLLSEPAKSQGRKNKLHKMTNKIENSYI
                     VVLDDSVVGEKGLYSIAPYIASDMAQSYKGSLKRVFQHAINGFAVEMSPQDAERLSED
                     FRVRFVEEDSVVTADVTQTGPPWGLDRIDQRNLPLSGTYTYNWTGAGVRVYVIDTGIR
                     TSHSQFGGRASNVFDAFGGSGSDCNGHGTHVSGTVGGSTYGVAKGSLLRGVRVLDCNG
                     SGSTSGVIAGVDWVRQNHIAPAVANMSLGGGISSALDTAVNNLSNAGVPIAVAAGNSN
                     TNACNSSPARAVNAITVGATTTSDARASFSNFGTCVDLFAPGQSILSAWFTSNTATAT
                     LSGTSMASPHVAGVAALYKQANPSASSTTVRDAIVNNATTGKVTNAGTGSPNRLLYSL
                     FF"
     gene            complement(1751..>2753)
                     /locus_tag="VFH91_02110"
     CDS             complement(1751..>2753)
                     /locus_tag="VFH91_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861802.1"
                     /translation="YRAITEKTARTELQSSGFGMEDFVRAIRYPFQHKAALFGGALIY
                     GFLLLAGFRGGLVAWMIMFGCISHVISQVAWGRLNRSFMPDFSAFSFWDDLIVPCFLG
                     LGIMIVSWGPVIALVLALMFGVMSGAGAESSSLHTGDHVAKSTGPSSDDLDVLLDPNA
                     DPAKLEAANNKLNETRPGAQIAREAQRSQDEEGDPAGMFRMLLPYLGAGIFIVLLFLV
                     LIGWGLFYYPMALTVAGFTQSFGSVMNPLVGLDTIRRMGATYFKAFAMVIVVLAVSTV
                     VGFIVSLITSPFNIPFMGNLPAHFINGTFTFYFNLVIACILGLSLFKCADRLGIDVD"
BASE COUNT          713 a          733 c          698 g          609 t
ORIGIN      
        1 ggttgagctt tggcaacccc ggatcacgaa tgtcatttgg tgggaacgtg caacccttca
       61 ggattgcgac aaaaacaatt caccatcttt atccccaggg ttgccaaagc tcaacccctg
      121 ggctgaaatt ggccaacacc ttcggtgtaa ccttgcggcg gaattggcga cttaatgcca
      181 ccggctccaa gcaaccgcga gactgagaaa gaattcaaac tgaaccagta tccagctttt
      241 ggtgaaccct tgctagtagt ttatttagtg atatactgcg cgcctcccgt ccagacaact
      301 tgtcccagaa cataggttcg ccccattaaa acctcgacgc taactcccgt cacgttcgca
      361 cgactctgtt cgtttcggcg agtattaaat ttgccgttcc tgagctttcg tcaggcgaac
      421 aagtggaagg aaccgcccga cctctcgcct agcgtgcggt ccgacctcaa accttactaa
      481 atggaggaca agaacatgaa aaagatcagc gcgctaatcg tcctggcctg cttttgtgcc
      541 gtaatgcttc tttcggagcc ggctaaaagt cagggtcgta agaataaact tcacaagatg
      601 acgaacaaga tcgagaacag ttacatcgtc gttctcgacg actcagtggt tggcgaaaag
      661 ggcctctatt caatcgcgcc ttacatcgcc tctgacatgg ctcaatctta caaagggagt
      721 ctcaagcgag tctttcaaca tgcaatcaac ggcttcgctg tagaaatgtc gccgcaagat
      781 gcagaacgac tcagcgaaga tttccgtgta cgattcgtag aggaagatag cgttgtgaca
      841 gccgacgtaa cgcagactgg accaccgtgg ggtcttgacc gaatcgatca aagaaatcta
      901 ccactcagtg gaacctacac ttacaactgg accggagccg gcgtcagggt atacgtgatc
      961 gatacgggca ttcggacgag tcacagtcag ttcggtggac gcgcttcgaa tgtattcgat
     1021 gctttcggtg gcagcggttc tgactgtaac ggacatggca ctcacgtctc aggaactgta
     1081 ggtggctcga cctatggtgt tgcgaaaggc tcgttgttgc gtggtgtgcg tgttcttgac
     1141 tgcaatgggt cgggttcaac gtcgggcgtg atcgcggggg tcgattgggt cagacaaaac
     1201 catattgcgc ccgcagtcgc gaacatgagt ctgggcggtg gcatttcgag tgcgctcgat
     1261 actgcggtta ataatctgtc caacgctgga gttcctattg cagtagcagc gggaaacagt
     1321 aatacgaacg cctgcaactc gtcgccggct cgagcggtaa atgcaattac agttggcgcg
     1381 acaactacga gcgatgctcg cgcgtcattc tcgaatttcg gtacttgtgt tgacctgttt
     1441 gcgccgggtc agtcaattct gtcagcctgg tttacctcaa acactgccac agcgacgtta
     1501 agcggaacgt cgatggcttc acctcatgtg gccggtgtcg ccgcgcttta caagcaagcg
     1561 aatccatcag cctcgtctac taccgttcgc gacgcgatcg tcaacaatgc tacgacgggt
     1621 aaagtcacaa acgccggcac gggctctccg aaccggctgc tttattcgtt gttcttctga
     1681 gcgttggacc gtaattagat tgtcgggagc cgttttagag atgcggctcc tgatgatctg
     1741 tttcgttgtc tcaatcgacg tcgatgccca gccgatcagc acatttgaaa agtgacagac
     1801 caagtatgca ggcgatgaca agattgaagt aaaaagtgaa cgtgccattg atgaagtgag
     1861 ccggcaagtt tcccatgaaa ggaatattga acggcgaggt tatcaaggag acgataaagc
     1921 ccaccacagt cgacactgcc agcactacaa tcaccatcgc gaaggcttta aagtatgtcg
     1981 cgcccatccg tcggattgtg tccagaccga caagcggatt catgacggat ccaaagcttt
     2041 gagtaaatcc tgcgactgtc aacgccatgg ggtaataaaa caaaccccaa cctatcagga
     2101 ccagaaacag caagacaata aagatcccgg cgcccagata gggaagtagc attctgaaca
     2161 tgcccgcagg gtcgccctct tcgtcctgtg agcgctgcgc ttcacgtgca atctgagctc
     2221 ccggacgagt ctcattcaac ttgttgttgg cggcttcgag cttcgcggga tctgcattgg
     2281 gatccaacag tacatcaagg tcgtccgaac ttggaccagt agacttcgcg acatgatcgc
     2341 ccgtatgcaa ggatgatgac tcagctccgg ccccgctcat cactccaaac atcaacgcga
     2401 gaaccaacgc gatcactggt ccccaggaaa caatcatgat tcctaacccg agaaagcagg
     2461 gaacaatcag atcatcccaa aatgaaaagg cactgaagtc aggcataaag ctacgattga
     2521 gtcgtcccca cgcaacctga ctgatcacat gcgagataca gccaaacatg atcatccagg
     2581 ccactaaacc gccgcgaaag cccgccagta acaagaatcc ataaatcaat gcgccaccga
     2641 acaatgcggc tttgtgttgg aacggataac gaattgctcg aacgaagtct tccatgccga
     2701 agcccgaact ttgtaactcg gtacgagcag tcttctcagt aatggcacga tac
//
LOCUS       DASSFP010000100         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_468598, whole genome shotgun sequence.
ACCESSION   DASSFP010000100 DASSFP010000000
VERSION     DASSFP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468598"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            830..3385
                     /locus_tag="VFH91_02115"
     CDS             830..3385
                     /locus_tag="VFH91_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6861803.1"
                     /translation="MRTLSPRVAFLLLSLATCALSVSAVLAFDSSNQPLVSETTVGSE
                     GFAVVQSATLLQPTNTTPTHSGSNNHGPPFWDRFEFRIIVVACLVIFQSILITGLLVE
                     RSRRWRASRELAESEERYRNEQKRAETALRDAEERNLAILRAIPDLIFLQDKDGVYLD
                     YHPKDYQHLVVPAEKFLGKRMTDVLPPEIEVPMRKNLTRAISTGNLQLSEYSIEFEGK
                     RKWFEARTVPCNGNKVLTVVRDITQRKKTEAALVESEEFNRRIVESSTDCIKVLDLQG
                     NLLYISQNGLRLLEMTETEPYLNASWIDLWAGEAKTASADAVLRAAQGENVVFLADGT
                     TIGGKRKWWNTVITPIRGEDGKVERLLAVSRDVTDQKQAVEVVQESKERFAKAFNANP
                     QPMSLTTLNDGRYIDVNESFLEMSGYTREEVIGNTSLELSIFETPAHRSLLLEPLLLK
                     GEAKNLELKFRTKSGKFRVLLSSAELIDIKGEHCVLVASSDITERKELEENLRLSERE
                     FSTLVENSPDIISRLDRRLRYIYISPSLERITGAPTDRFIGKRQREAALPGYDWRDFE
                     DHCRKAFATGRTVTRAFEFKGRNYWTRIIPELSMKGEVESLMTISEDVTNRMRAEKEL
                     VDLTTRLFNIQDEERRRIARELHDGTAQNLFAISINLAKLMQLDQLKANEIEQLLVEC
                     QSLGEQSLKEIRTLSYLLHPPLLDQSGLVSALQWYVEGFSKRSGIYVDVIAQPIGRLS
                     SDVETALFRIVQEALTNVRRHSCSETASIRLERRIGEVQLEINDQGRGLPPINKARAD
                     GLPEMGVGIPGMKQRLRQLGGKLEITSSERGTSIVAVVPLKNGASLHANSASG"
     gene            3363..4007
                     /locus_tag="VFH91_02120"
     CDS             3363..4007
                     /locus_tag="VFH91_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6861804.1"
                     /translation="MRILLADDHDIVRRGLRDQLTSREGWEVCGEATNGREAVAMALK
                     LKPDVVVLDLSMPELNGLEATRQIRRELPRTEVLIFTMHETEQLIREVLAAGARGYVL
                     KSDAGRHLTSAVEALSHHKPFFTAKVSEALLDAFLKSNVAPDEGSVFRTLTDREREIV
                     QMLAEGKSNKEIASKLSISVKTVETHRATVMRKLEINSIVELVHYAIRNQLVEA"
BASE COUNT         1102 a         1045 c         1071 g         1037 t
ORIGIN      
        1 tgctcgagtt ctgaacctcg attctccaag cgacacgtct ccgttaaagt tgcgccaagc
       61 tattgcgata gacttcgtca aatttcgcct gtctttaacc tcttagggac ggcgcaaact
      121 gctacccact tgtcctcacc tgggtgattt cgagggtctc tttctgtctt gagtgattcg
      181 caaggttaag taattgacct cagtgcggta atcaggtgaa atgcgtattt cgtggcggga
      241 ggatctgtag taatggtgaa gaatcatcat ttcacttcgt taattgggca ggctttgcac
      301 aaaaagtcgg ttttagccga cccgcgagcc agtacagaac cgcaactgtc cgatatgctt
      361 tcgcctgtcg gtcgtcgtta actcgtttct ctgagctttg gtttgcatca tgaccgacaa
      421 gctaaagcat atcggacagt cgcggttctg tactggctcg cgtcttactt tttggtctcg
      481 acgactacga cgcttagtag tagtgattca ggcgttattt tcggccacct acagtaattt
      541 ttcggggttg aaagtatgag agaccgagcg tacactcccg cccatagctg aacaagaaaa
      601 gagcgactaa ggacgacgag gacgaccctt aattttcaac ggattcgtgt ccagtctgct
      661 ctccagagat atctcagaat cgagcagctg aagttcaaat caactgccgt gtgacagaca
      721 atcaagggcc cagagctttc ccctaaagtg cccctactcc gcgaactata taggggttta
      781 acgacagtaa tgagtaattt gagccagacc ggccctttgc gcggcaacta tgcgaacgct
      841 ttccccacga gtcgcgtttc tacttctcag tcttgcaacg tgtgccctgt cggtttctgc
      901 ggttctcgcg tttgattcat ccaaccagcc tttagtatcc gaaaccaccg ttgggagcga
      961 aggtttcgcc gtagtccaat ctgcaacctt acttcaaccc actaacacaa ctcctactca
     1021 ctctggttca aataatcatg gtccaccttt ttgggatcgc tttgaattta gaattatcgt
     1081 cgttgcctgc ctcgtcattt ttcaatcgat cttgatcaca ggtctgctgg ttgaacgcag
     1141 cagacgttgg cgcgccagcc gcgaacttgc tgaaagcgaa gaacgttacc ggaatgaaca
     1201 aaagagggca gaaaccgcgt tgcgagatgc tgaagaaaga aatctcgcta tccttcgagc
     1261 gattcctgat ttgattttcc tgcaggacaa agatggggtc tatctcgatt accacccgaa
     1321 ggattatcag catctggttg tgcctgcgga aaagttcctg ggcaaacgga tgaccgacgt
     1381 attgccgccg gagattgaag tgcccatgcg caagaacctc actcgcgcta tcagcacggg
     1441 aaacttacaa ctgtccgagt attcgatcga gtttgaaggt aaacgcaaat ggtttgaagc
     1501 gcgcaccgtt ccctgcaacg gcaacaaagt gcttaccgtc gtccgcgata ttacccagcg
     1561 taagaaaact gaagcggcat tggtcgaaag cgaagagttc aaccggcgta ttgtcgaaag
     1621 cagcaccgac tgcataaaag ttttggatct ccagggaaac cttctttaca taagtcaaaa
     1681 cggactgcgc ttgttggaaa tgactgaaac tgaaccgtac ctgaacgcgt catggattga
     1741 cctttgggca ggtgaagcga aaaccgcctc agccgacgca gtactacgtg cagctcaagg
     1801 tgaaaatgtt gtgttcctgg ccgacggcac tacgattggc ggaaaacgaa aatggtggaa
     1861 tacggtcatt actcccattc gaggtgaaga cggcaaagtc gagcgtttgc ttgctgtatc
     1921 tcgcgatgtt acggatcaaa aacaagctgt ggaagttgtt caggagagta aggaacgctt
     1981 cgctaaggca ttcaacgcca atcctcagcc aatgtctctt acgactttga atgatggacg
     2041 ctatatcgac gtcaacgaaa gtttcctgga aatgagtggt tatacccgtg aagaagttat
     2101 cgggaacact tcactggagc tttcaatctt tgaaactcca gcccatcgtt ctttgctgct
     2161 tgagccactg ttgcttaaag gcgaagctaa aaatctggaa ctgaagtttc gcaccaagag
     2221 tggtaaattt agagttctgc tttcctcagc cgaattaatc gatatcaaag gtgaacactg
     2281 cgttctcgta gcctcgagcg atatcacgga gcgtaaggaa cttgaagaaa atcttcgctt
     2341 aagcgaacgc gagttttcga ctcttgtcga gaactcgccc gatatcatct cccggttgga
     2401 tcggcggctg cgctacatct atatcagtcc ctcgctggaa cggatcacgg gtgcacctac
     2461 ggatcgattt attggcaagc gtcagcgcga ggcggcgcta ccaggttatg actggagaga
     2521 cttcgaagac cattgtcgga aggccttcgc gactggaaga actgtgacca gagcgttcga
     2581 gtttaaagga cgcaactact ggacacgcat catccctgaa ttgtccatga agggtgaagt
     2641 cgaatcatta atgacaatta gcgaagatgt tactaaccgt atgcgcgcag aaaaggaact
     2701 ggttgacctc acgaccagac ttttcaatat tcaggatgaa gaacgccgcc gcattgcccg
     2761 cgagttgcac gacggaacag cacaaaatct ctttgccatt tcgattaatc tcgccaagct
     2821 catgcaattg gatcagttaa aagctaacga gattgaacag ctacttgttg agtgtcagtc
     2881 attgggcgag cagtcactaa aggagatacg gaccttatct tacctactgc atccaccgtt
     2941 actcgatcaa tcgggtttgg tttcggcgct gcagtggtac gtcgaaggtt ttagcaagcg
     3001 cagtggcatc tacgtcgacg taattgctca accaatcggc cgactttcat ctgacgttga
     3061 gaccgcgctc ttccggatcg tccaggaggc cctcaccaat gttcgacgtc actcctgcag
     3121 tgaaacagca agtatccggc tcgaaagacg aataggggaa gttcaactcg agatcaacga
     3181 tcagggtcgc ggtctgcccc cgattaataa agccagagca gatgggctac ccgagatggg
     3241 tgtgggtata ccgggcatga aacagcgctt gcgccaattg ggtggcaagc tggaaatcac
     3301 ttcaagcgaa cgtggaacct cgatagttgc agtagtgccg ctgaaaaacg gagctagtct
     3361 tcatgcgaat tctgctagcg gatgatcacg acattgtccg tcgcggctta agagatcaac
     3421 ttaccagtcg ggaaggctgg gaagtgtgcg gcgaggcaac aaatggacgc gaggctgttg
     3481 ccatggcgct gaaactcaag cccgacgtcg ttgtgttgga tctatcaatg cctgaactca
     3541 acgggcttga ggcgacgcgt caaatccgac gcgagttgcc gcgcacagag gtactgatct
     3601 tcacgatgca tgaaacagaa caactgattc gtgaagtcct ggcggctgga gctcgcggtt
     3661 atgtgctcaa atcagacgcg gggcgtcacc ttactagtgc agtggaagca ctttctcatc
     3721 acaagccatt ttttaccgca aaggtttcag aggctttgct cgacgccttt ctgaagtcga
     3781 atgtggcgcc cgatgagggg tccgtcttta ggactttgac tgatcgtgaa cgggaaattg
     3841 tgcaaatgct ggcggagggg aaaagtaaca aggagatcgc ctcgaagctc tccattagtg
     3901 tgaaaacggt cgagacccat cgagcaactg taatgcgaaa actcgagatt aattcgatcg
     3961 ttgaattagt ccactatgcg attagaaacc aactcgtaga ggcgtagtcc ctcaactttg
     4021 cggagcggtt tacataatca tcctaccctg aaatacagac tctaccgtat accttaagac
     4081 acccaaaaaa actattctcc cactgatacc aacgacagtt acgcttaacc gaaagagtaa
     4141 ctgggtaaac ctttcaccgt tcatatctga ggataatcga ttctttgtga cctcgacgta
     4201 atcgctggcg ctcttcgcag caactacaac gtacaggcaa cctcagattg ggggt
//
LOCUS       DASSFP010000101         5246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_319061, whole genome shotgun sequence.
ACCESSION   DASSFP010000101 DASSFP010000000
VERSION     DASSFP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319061"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFH91_02125"
     CDS             <1..313
                     /locus_tag="VFH91_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861805.1"
                     /translation="PEVVRDLVRGIAESGEWAETHRIEAAKVVSPYFRQDEKVVRYVL
                     TQPPDRVSYRMLTPTDAELQVIHDMALKAGILEKPISMKDLIDREFIPADIKPANIQV
                     H"
     gene            369..3911
                     /locus_tag="VFH91_02130"
     CDS             369..3911
                     /locus_tag="VFH91_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET6861806.1"
                     /translation="MSKRILGTYIILVVSVGVALTFLPHSLAEPQSPAAPREDAYRAN
                     NLGVALLEQFKYKEGADAFRRALQLDPKLALARINLSIALFNVPDLPGAQREAQAAIA
                     LAPNAPQPHYILGLIAKSQARIDDAIAPFQQVLKLDPEDVGANINLGQIYSQQKKYPE
                     AIAAFRVALAVEPYNSTALYNLGTALIRAGQRDEGQRVIQRFQEFRTRGSGSSLGNNY
                     LEQGRYAEAVASTGAEADLVDKTAPSNTFSDSTSEVFGSGSPKASDIRASMLGGRFRS
                     SEMNDQKRNSIVAALGGSATLFDFDGDGDMDLLWITASEQHLYRNDAGKFTEVTAGSG
                     GLATKVTAANVAAVAGDYDNDGRADLFVVRDSSLALYHNDGNGKFSDVSSAAGIPSYP
                     YLPGSVALVDVDHDGDLDLFVAGLADISKPPKVDEPLFPGDFPAAPNLLLRNDGNGKF
                     TDITSTAKAGDAGHAVAIIPTDFNNRRDIDLFVLNYGKAPSLLSNQRDGSFRDVAGDV
                     GLGTSGNWSCAAAGDLNKDGFTDFFMGRVDGPGVFAMSDGHEKFKSVPAPAVTDKARA
                     AQFVDYDNDGLIDCLVLTDKGLIVLRNLGSSWDDVSGRAVGTGLSTVGFGGSRLLASA
                     DLDNDGDVDILFASPAGELRVGRNSGNDNHSLRIRLEGKVSNRSAVGVKIEARAGSLV
                     QKLETYSAWPAPTSADVTVGLGKRETVDAVRILWPAGIVQAETEFAKSTRPSLLTLNV
                     TELDRKPSSCPFLYSWNGERFEFITDFMGGGEMGYLEEPGRHNTPDPVEYVRIRDDQL
                     KEKNGKFELRVTNELEETLYADRLQLMAIDHPDKMDVYPNEGMTDPPKSFKLFVTKGA
                     SPPLSAIDNHGTDVLDRVAKLDRTYPDNFKLDRIRGYAESHSLTLKLSEPQQTIRNER
                     TLLLLTGWTDYAWSSDNVAASQGKKEMTLPALQVKDRRGNWQTVIDDIGIPVGRPQTV
                     TVDLTGKFLSSSREIRIVTNMRIYWDQILVDHSNGNFQTRVTRLDPSSANLRWRGFSK
                     EVTPDGREPLGYDYSKVSFTSPWKVMTGYYTREGNVLALLTGSDDMFVIARPGDEISL
                     AFDADRLPTLPGGWSRTFILYSDGFSKEMDINSASPDQVGPLPFHGMSKYPYSSPEAY
                     PMTKDRQAYIERYNTRLVTDAVASIETELLLRSR"
     gene            4017..5036
                     /locus_tag="VFH91_02135"
     CDS             4017..5036
                     /locus_tag="VFH91_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6861807.1"
                     /translation="MRLFIICVVLSLLSLTTLAQDQQFANLGDFKLESGEIIHDCRIG
                     YRIFGKLNADKSNAVILPTWASGTTEQLKSSVGPGRMLDEKYFVIAVDALGNGVSSSP
                     SNSKLQSRMRFPKFTLRDCVRSQHELVTGVLKLTHVRAVVGVSMGGMQAFEWLVSYPE
                     FMDMAVPIVGSPRLAPYDLLLWQAQIDAIMNDRGWNNGEYITNPARSAEYEFGALLLT
                     TPANYNNQMTREKILAELKKAAEQKGGGFDANDKIRQDQAMMAHDIAAPYGGSVEKAA
                     ARVKAKVMVVVSRYDHVVTPGPASEFAKLINAPLLELLGDCGHLAPSCESQTVNSAIA
                     RFLDK"
     gene            5033..>5246
                     /locus_tag="VFH91_02140"
     CDS             5033..>5246
                     /locus_tag="VFH91_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861808.1"
                     /translation="MMIYARLAAEMEIVLQHDVLDVWFPRVIDKQNGGFLSSFTRDWK
                     PDQSDGKFSVFQGRMVWVAAEVLKRRP"
BASE COUNT         1287 a         1324 c         1388 g         1247 t
ORIGIN      
        1 gcctgaagtg gtgagagacc ttgttcgtgg catcgctgag agtggagaat gggccgaaac
       61 gcatcgcatc gaagcggcca aagttgtgtc accatatttt cgtcaggatg aaaaagttgt
      121 tcgttatgtt ttgactcagc caccggatcg agtgagctat cgaatgttga ctccgaccga
      181 tgcagagttg caggtcatcc acgatatggc cctcaaagca ggtattctcg agaagccgat
      241 cagcatgaag gatctgattg atcgtgagtt catccccgcg gacataaagc ccgcgaatat
      301 tcaggtgcac tagtgggggg agagaatagg acctattaag tggcattata gggccgcaaa
      361 aattgaaaat gagcaagcga atattaggta cctacattat cctcgtcgtg tcggttggcg
      421 tcgctctaac tttcctgccg cattcccttg ctgaaccaca atcgcccgct gcgccgcgag
      481 aagatgccta tcgtgccaac aatctcggcg tcgctctgct cgagcaattc aagtacaagg
      541 aaggtgctga tgcgtttcgt cgagcacttc aacttgatcc aaaactcgct cttgcacgaa
      601 ttaatctcag catcgcactc ttcaacgtgc ccgatcttcc tggggcgcaa cgtgaagctc
      661 aggccgcaat tgcgctcgct cctaacgccc cgcagccgca ctacatttta ggtctaatcg
      721 cgaagtctca ggctcgcatc gacgatgcga tcgcaccctt tcaacaagtc ctgaaacttg
      781 atccggaaga tgtcggcgcc aacatcaacc tgggccagat ctactcgcaa cagaagaaat
      841 atccagaggc aattgctgct ttccgagtcg cacttgctgt tgaaccttac aactcaaccg
      901 ccctttataa cctgggcact gcgctcatcc gcgcaggtca gcgagacgag ggacagcgag
      961 tgattcagcg gttccaggaa tttcgcactc gcggaagtgg ctcgtcactc ggcaacaact
     1021 atctcgagca gggacgttac gccgaagctg ttgcctcaac tggagccgag gcggacctgg
     1081 ttgacaagac ggctccctca aatacttttt ccgactcaac tagtgaagtg tttggttccg
     1141 gctcaccaaa agcgagtgac attcgcgcgt cgatgctggg tggtagattc cgcagcagcg
     1201 agatgaatga ccagaagcgt aattcaatcg tagccgctct cggcggtagt gcaacgcttt
     1261 tcgattttga tggcgacggc gatatggatc tcttgtggat taccgcatcc gaacaacatc
     1321 tctatcgcaa tgacgcggga aaatttactg aagtgactgc tggttcaggt ggtttggcga
     1381 ctaaagtcac tgccgcaaac gtcgcagctg tcgccggtga ctacgacaat gatggtcgag
     1441 cggatctgtt cgtcgtacga gatagtagtc tcgcgctata tcacaatgat ggaaatggca
     1501 aattttccga cgtctccagc gcagcaggca ttccctcata tccttatttg ccgggttcag
     1561 tggcgcttgt agatgtggac cacgatggtg atctggatct ctttgttgcc gggcttgcag
     1621 acattagcaa gccaccgaag gttgatgagc cgctatttcc cggtgacttt ccagccgctc
     1681 caaacctgct cttgcggaac gacggcaatg gaaaatttac tgatattaca agcacggcga
     1741 aagcaggcga cgcgggtcat gcagtcgcaa ttattccaac cgacttcaat aaccgccgcg
     1801 acatagatct atttgtctta aactacggaa aagccccatc tttactcagt aatcaacgag
     1861 acggatcttt tcgagacgtt gccggtgatg ttgggctggg gacgtctggg aactggagct
     1921 gcgcagcggc aggagactta aacaaagacg gtttcacaga tttctttatg ggccgcgtgg
     1981 atggtccggg cgtgtttgcc atgagcgatg gtcacgagaa attcaaatcg gttccagcgc
     2041 cggcagtaac cgacaaagcc cgagccgcac agtttgttga ttacgataac gatggcttga
     2101 ttgattgcct tgttttgact gacaaggggt taatcgttct gcgcaatctt ggaagctcat
     2161 gggatgacgt ctctggccgt gcagtgggca ctggtctttc aactgttgga tttggtggct
     2221 cgcgcctact ggcctccgct gatctcgata atgatggcga cgttgatatt ctcttcgcat
     2281 caccggcggg cgaacttcgt gtgggtcgca acagcggaaa tgacaatcac tcacttcgga
     2341 taaggctcga gggtaaagtc agtaaccgga gtgccgttgg agttaagatc gaagctcggg
     2401 ccggaagttt ggtccaaaag ctcgaaacct attcagcctg gcccgcgcca acttccgccg
     2461 atgttaccgt cggattgggt aagcgcgaga cagttgatgc cgtcagaatt ctttggcccg
     2521 ccggaatcgt tcaggcagag acggaatttg cgaagagcac tcgtccgtcg ctactaacac
     2581 taaatgttac ggagctggat cgtaaaccat cgtcctgtcc attcttgtat tcatggaacg
     2641 gcgagcggtt cgaattcatc accgacttca tgggtggcgg ggaaatggga tatctcgaag
     2701 aaccgggtcg tcacaacacg ccggatccgg tcgaatacgt acgcatccgc gacgatcaat
     2761 taaaggaaaa gaacggcaag ttcgagttgc gcgtgacgaa cgagttagag gaaaccctgt
     2821 atgcagatcg actgcaattg atggctatcg accatccaga caagatggac gtctatccaa
     2881 acgaaggaat gaccgatccg cctaaatcgt tcaagctttt tgttactaaa ggtgcgagtc
     2941 cacccttgag cgcaatcgac aatcatggaa cggatgtgct cgaccgggta gcaaagcttg
     3001 atcgtacgta tcctgacaat ttcaagttgg accgaattcg agggtatgcg gaatctcact
     3061 cattgactct caagctctcg gaacctcagc agacaattcg taatgagaga actcttctgc
     3121 tgctaaccgg atggaccgat tacgcgtggt ccagtgataa cgtggcggct tcgcaaggga
     3181 agaaggaaat gacgctccct gcgttacaag tcaaagacag acgtggtaat tggcaaactg
     3241 tgattgatga cattggcatt cccgttggtc gcccgcaaac agttacagtg gatctcactg
     3301 gcaagtttct cagttcaagt cgtgagatcc gtattgtcac taacatgaga atctactggg
     3361 accagattct ggtggaccac tctaacggta attttcaaac tagagtaact cgtctcgatc
     3421 cgtcctccgc taacttacgt tggcgcggct tttcaaaaga agtaactccc gatggacgtg
     3481 aaccattggg ttacgattat tcgaaggtgt ctttcacttc accgtggaaa gtaatgacgg
     3541 gctattacac tcgcgaagga aacgtgttgg cactgctgac tggttcggac gacatgtttg
     3601 tgattgcccg tcctggagac gaaatcagtc ttgcattcga tgctgacaga cttccaacac
     3661 tacctggtgg ttggagtcga acctttattc tctattctga cggattcagt aaggagatgg
     3721 atataaattc ggcgagtccg gaccaggttg gaccacttcc tttccacggg atgtcgaagt
     3781 atccgtactc gtcgccggaa gcttatccga tgacaaagga tcggcaggct tatatcgaac
     3841 gatacaacac tcgattggta acggacgcgg ttgcctcaat cgagactgaa ttgctactga
     3901 gatccagata acatccgcag cttacgccga ttacaccgaa accaatgcgg tgcctacatc
     3961 aaagcggcca atgcagtgct tacatcagct gtgtgatcgg cgaataaccg actaaaatga
     4021 gactcttcat tatttgcgtc gtcctgtcac ttctttcact cacgactctt gctcaggacc
     4081 agcagttcgc taacctcggt gacttcaaac ttgaaagcgg agagatcatc cacgattgcc
     4141 gaattggcta tcgcatcttc ggcaaactca acgctgacaa atcgaacgcc gtcattctcc
     4201 caacctgggc ctctgggaca accgaacaac tgaaatcgag cgtcggtccg ggaagaatgc
     4261 tcgacgagaa gtatttcgtg atcgccgttg atgcgcttgg aaatggtgtc tcctcgtcac
     4321 cttccaacag taaattgcag tcacgtatga gatttccgaa gtttacattg cgcgattgtg
     4381 ttcggtcgca gcacgaactc gtgacaggag ttcttaagtt gacacatgta agagcggtag
     4441 tcggtgtctc aatgggcggc atgcaagcgt tcgaatggct ggtttcctat cctgaattta
     4501 tggacatggc cgttccgatc gtcggatcgc cgcggctagc tccctacgat ctgttgctct
     4561 ggcaggcgca gattgacgcc atcatgaatg accgtggctg gaacaatggc gagtacataa
     4621 ccaacccggc gcgatcggca gaatatgaat tcggcgcatt actattaact acgcccgcca
     4681 attacaacaa ccagatgacc cgcgaaaaga tacttgcgga attgaagaaa gccgccgagc
     4741 aaaaaggcgg cggttttgat gcaaacgaca aaattcgaca ggaccaggcg atgatggccc
     4801 acgacatcgc tgcaccctac ggcggctcgg tggagaaagc cgctgcacgt gtaaaagcta
     4861 aagtcatggt ggttgtctcg cgttacgatc acgtcgtgac acctggacca gccagcgaat
     4921 ttgccaaact tatcaacgca ccgctgctcg agttgctcgg tgactgcgga catctcgcgc
     4981 cgtcgtgcga atcgcagaca gttaactccg ccatcgcaag atttttggat aaatgatgat
     5041 ctatgcaagg ttggcagccg aaatggaaat tgtgctccag catgacgtgc tggacgtttg
     5101 gtttccacgg gtcatcgaca agcagaatgg tggtttcctt tcaagcttta cgcgtgactg
     5161 gaagcctgac cagtccgacg gaaagttctc ggtttttcag ggtcgcatgg tttgggttgc
     5221 ggctgaggtt ctgaagcgcc gccctg
//
LOCUS       DASSFP010000102         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_915690, whole genome shotgun sequence.
ACCESSION   DASSFP010000102 DASSFP010000000
VERSION     DASSFP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915690"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VFH91_02145"
     CDS             complement(<1..1274)
                     /locus_tag="VFH91_02145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6861809.1"
                     /translation="MTDCKECGGKVAPGEHFCGNCGAQLPAGSPELETVAVTLDPEVV
                     VDHSPVKTESKVDSHADPEPDLAVPKAVGSATPSREKSKYTAAADPVDSLSGTDPGAQ
                     ISSSSLGGSATDDVRIFGTSSTPQKGATGGHHASVKQLESGTVLNSRYEIVRRIGGGG
                     MGAVYLAKDRNLGDAPRAVKEMVESHLDPAQHEKAIGDFKRESLLLTSLEHPSIPTIY
                     DYFYDDALSRFYLVMKYISGGDLASRLRAAVGGRLDEKTVTDWAMQIADVLDYLHSRP
                     KAIIYRDLKPANLMIDGNTSRVMLIDFGIARWVSQQEKGVTAVGTMGYAPPELFSGRV
                     QAASDIYSLGATMFHLLTGSDPQDNPLLIFDFSKNPRPRQIAPGISAEMEQILMRTVE
                     YKPEDRFRTAGELRNELAIHLEKLMSGRVNYGV"
     gene            complement(1341..1814)
                     /locus_tag="VFH91_02150"
     CDS             complement(1341..1814)
                     /locus_tag="VFH91_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6861810.1"
                     /translation="MLSPKNYPNGGNGQNGSRTNLAGAHPEFAGGHVSGNLNLAGVPP
                     ATKPHARLVIEKGRSTGKQFMLSDLESQIGRWDADGGIFPDVDLDTDDPEAKVSRRHA
                     RITLSNGQYFLEDLGSTNGTFINRGKRLAPGQRQALCDGDEIIVGKTFLRFHIVK"
     gene            2386..>3728
                     /locus_tag="VFH91_02155"
     CDS             2386..>3728
                     /locus_tag="VFH91_02155"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6861811.1"
                     /translation="MALSRKKKIIIGVSVAVVLAIVVIVSVYATRKDEPEVITVKIET
                     RPELRQTVTASGEVRPIRYIKLTSEVPGRIEEIYVNAGDQVIKGKPLVRVDPTQLQST
                     QEAQWAATQASINDVQNARNAVSSAQQGLVVAESSVAAARQQVVAMQTNVDRAQVDLN
                     TAQRELKRYTDLIEAGVSSRLEYDTARDRFETAKISLETARANLEAQRIAVKEATERA
                     NQQKIAVRDAQTAIKSSEMRANQQAAMLRGQTSQRSKATQLSPLNGVVADIPTRVGEY
                     AVAGLSTTPLMTIADMSQINVEVNVDETEINNVEVGQQAKVKVDALGDKEIKAVVTQK
                     NPLAIAKSDQTGGLSNRVNVQEAKEFKVTLQLQELPDEVQTSLRPGMSSTATITTKTK
                     NNVLAVPLESIVEKSPTPNASPSPSIAGNTPTPVSSDKPKPQKGVYLIEGNKVRFV"
BASE COUNT          961 a          981 c          933 g          853 t
ORIGIN      
        1 actccgtaat taactcggcc cgacatcaat ttctcgagat ggattgccag ctcgtttcgc
       61 aattcacctg ccgtgcgaaa acgatcttca ggcttatatt cgactgttct catcaagatc
      121 tgctccatct ctgccgagat accgggagcg atctgcctgg gccgtggatt cttggaaaaa
      181 tcgaagatca agagtgggtt gtcctgcgga tccgatccag taaggagatg aaacatcgtc
      241 gcccccaaac tgtaaatatc ggacgccgcc tgaactcgac cactgaacag ttcgggcggc
      301 gcgtaaccca tggttcctac cgccgttacg cctttctcct gttgcgacac ccaccgcgca
      361 attccaaaat caatcagcat tactcgactg gtgttgccgt cgatcatcaa attcgcgggc
      421 ttcaggtcac ggtaaataat tgctttcggt cgcgagtgga gatagtccag tacatcggcg
      481 atttgcattg cccagtcggt tactgtcttc tcatcgagtc gcccacccac agccgcacgt
      541 agtcgagagg ccagatcgcc tccagaaatg tatttcatga cgagataaaa acggctgagc
      601 gcatcgtcat agaagtagtc gtaaatcgtc ggaattgatg ggtgctcaag tgatgtgagt
      661 aacaacgact ctcgtttgaa atcgccgatc gctttttcat gttgagctgg atccagatgt
      721 gattccacca tctccttcac agctctcggt gcatcaccca ggtttcgatc cttagcaaga
      781 taaacggcgc ccattccacc gccaccgatt cggcggacga tctcataacg agaattcaga
      841 acagtgccac tctcgagttg tttaacggag gcatgatggc cgccagtagc acctttttgt
      901 ggcgtcgaac ttgtgccgaa aattctaacg tcatcagttg cacttccccc aagcgaactc
      961 gaagaaatct gtgcacccgg gtcagtacca cttagcgagt cgacgggatc agcagccgcc
     1021 gtgtacttcg acttttcacg agaaggcgtt gctgagccaa ccgcttttgg caccgccaaa
     1081 tctggttctg ggtcagcgtg agagtcaact tttgactcgg tcttaaccgg gctatgatcg
     1141 actacaacct caggatctag agttacggct acggtctcta attccggcga ccctgcgggc
     1201 agttgtgcgc cgcagtttcc gcagaaatgt tcaccaggtg cgactttgcc gccgcattcc
     1261 ttacagtcag tcatctcttt gctatagtga aatcgagctc gccaacacca gtccgataag
     1321 ttaactcaca acggtcggtt ttatttcacg atgtgaaaac gcaagaaggt tttaccgacg
     1381 ataatttcgt ccccgtcgca gagggcttgg cgctgccctg gcgccaaccg ttttccgcga
     1441 ttaataaagg tcccgtttgt agacccgaga tcctcaagga agtactgacc attgctcaag
     1501 gtaatacggg cgtgacgtcg agaaaccttg gcttccggat cgtcagtatc cagatccaca
     1561 tccggaaaga ttccgccatc cgcatcccaa cgccctatct gcgactcaag gtcgctaagc
     1621 ataaattgct ttccggttga cctacccttt tcaatgacca atcgagcatg cggcttggtc
     1681 gccgggggaa cgcctgctag gttgagattg cccgaaacat gcccgccggc aaattccgga
     1741 tgagcgcccg ccaggttcgt tcgcgagccg ttttggccat ttccgccgtt tggataattc
     1801 ttgggggaaa gcacctggtg gccgttgggg cgcaaagggg cgccgcactc atcacaatat
     1861 tgcgagccct caaggttctc agttttgcac cggtcacagg taatcatgga ttgtctagtt
     1921 gctcaatccc ccaggaaaga ttgcctaagc aaaacagagc gggagaagaa ttttcatttt
     1981 agcgcgaaat gaaatggtgc gccaacgcgt cttatacagg ggagcccaga ttgggcacac
     2041 tatttttccg ttttcaggcc gtgggaacct gaccttcatc ggtggtcggt ggtcctcggt
     2101 tcgccggaag taattaaaat aaggcgagat agctggcctt tgctgcatca aatctgtgcc
     2161 gcgcaatgtt gaccaggatg ggtcaagact ggcaatctaa cgacaaacct gtaagcagct
     2221 ttgtgcctcc aacctacggc attggttgcc gttatatgaa actgtttggt aagttttgac
     2281 gtagaagaca cttcgagcgt aacggtttga cgttaagcgt tcccgataac acccacaacc
     2341 ctcgtttcga atgagaattt agcccctaac tgaggatttt aaaagatggc cttgagtcgt
     2401 aagaagaaaa ttattatcgg agtatcagta gcagttgtat tggcgattgt agtgatcgta
     2461 agcgtctacg ctacccgcaa agatgagccc gaagtcataa ccgttaaaat cgaaacccga
     2521 ccagaactca gacaaaccgt gaccgcttca ggtgaggttc ggcccatccg ctacatcaag
     2581 ctgaccagcg aggttccggg gcgcattgaa gaaatttacg tcaatgctgg ggatcaagtg
     2641 atcaaaggca agccgttggt ccgcgtcgat ccaactcagt tgcagtcaac ccaggaagca
     2701 caatgggctg caacccaggc ttctattaac gacgttcaga atgctcgcaa tgctgtttct
     2761 tccgcgcaac aaggcctggt agttgcagag tcctcagtag ccgcggcacg ccaacaggtc
     2821 gttgcgatgc agactaacgt ggaccgcgct caggtcgact taaatacagc ccaacgtgaa
     2881 ttgaaaagat atacagactt gattgaagca ggtgtttcat ctcggcttga atacgatact
     2941 gctcgagatc gtttcgaaac tgcgaagatt tcactcgaaa cagcccgggc gaatcttgaa
     3001 gctcagcgga ttgccgttaa agaagcaact gaacgagcta accagcagaa gattgctgtt
     3061 agggatgcac aaactgcgat caagtcctca gaaatgagag ctaaccagca agcagccatg
     3121 ctgcgaggtc aaacaagcca acggtccaaa gcaacacaac tttctccgtt gaatggtgtc
     3181 gtcgcggaca ttccaactcg cgtcggtgaa tatgccgtcg ccggtttatc aacaacacct
     3241 ctgatgacta ttgcggacat gtcccagatc aacgtagaag tgaacgttga cgaaaccgag
     3301 atcaataacg tcgaggtggg acaacaggcg aaggtgaaag tagacgccct gggcgataaa
     3361 gagatcaagg cggttgtaac acaaaagaat ccactggcaa ttgccaagtc ggaccaaaca
     3421 ggcgggctct cgaatcgagt gaatgtgcag gaagcaaagg agttcaaagt aactctacag
     3481 cttcaggaat tgccagacga agtgcagact tctctgcgac ctggaatgag ttcgaccgca
     3541 acgattacca cgaagacgaa gaacaacgtc cttgcggtac cgcttgaatc gattgtcgaa
     3601 aagtcgccaa cacctaatgc gtcgccatca ccgtctatcg cgggcaacac accgacacca
     3661 gtctccagcg acaaaccaaa accgcaaaaa ggtgtctatc tcatagaagg gaacaaggtc
     3721 cgctttgt
//
LOCUS       DASSFP010000103         4630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1065467, whole genome shotgun sequence.
ACCESSION   DASSFP010000103 DASSFP010000000
VERSION     DASSFP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065467"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VFH91_02160"
     CDS             complement(<1..165)
                     /locus_tag="VFH91_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6861812.1"
                     /translation="MKQKSHITFTLNDEQVEVAFAPHKTLLEVLREDLNLTGTKHGCE
                     LGECGTCAILV"
     gene            complement(266..1150)
                     /gene="panC"
                     /locus_tag="VFH91_02165"
     CDS             complement(266..1150)
                     /gene="panC"
                     /locus_tag="VFH91_02165"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET6861813.1"
                     /translation="MEIINRRQRMSSVARKIRRVQDRTIGLVPTMGALHEGHLSLVRE
                     ARRMCDVVIVSVFVNPTQFGPNEDFTRYPRDLTKDTALLTDYNVDYIFAPAVEEIYPR
                     GFSTYVNVEGLADQLEGASRPGHFRGVATIVTILLNTTRPDFAFFGQKDAQQALIIRR
                     LVKDLAFDTEIVVLPTVREDSGLAISSRNLYLNAEEQTAATVIHQSLGRAKTAYKDGE
                     RNGGRLEALIRSTIEVEPRARVDYVTVADAETLERIEKLDERPILIAVAAYIGKTRLI
                     DNIILNKVKKKEAVGLNA"
     gene            complement(1166..2044)
                     /gene="panB"
                     /locus_tag="VFH91_02170"
     CDS             complement(1166..2044)
                     /gene="panB"
                     /locus_tag="VFH91_02170"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET6861814.1"
                     /translation="MKPERPEKVSAPSLRSSKQREEKLVCLTAYDYPTARIVDEAGID
                     IILVGDSLGNVVLGYGNTVPVTLEEILIHLKAVRRAVQRALLVADMPYGTFHTGDDDA
                     VRNALRLVKEGGAEAIKLEGGHKRVHLVKRLVDEEISVMGHIGLTPQSINQLGAYRVQ
                     GKTAETARQLLDDAQAMEDAGAFAVVLEVVPREIAQMITESLSIPTIGIGAGSHCDIQ
                     VLVIHDMLGLSFGKQARFVRPYANLREVMTDAVTRYADDVRNGTYPSDAESYALPADA
                     AEALDIQTPTKNLKES"
     gene            complement(2191..2688)
                     /gene="folK"
                     /locus_tag="VFH91_02175"
     CDS             complement(2191..2688)
                     /gene="folK"
                     /locus_tag="VFH91_02175"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET6861815.1"
                     /translation="MIPNAYVGLGSNLGDRAGNLLLAVRGILDAGLEIIRLSPIYETE
                     PIGDLAQSYFLNQVAEVRGKSLPPPEQMLARLLRVEYALGRTRDVPNGPRTIDLDLLL
                     YKSEIRDTEFLRLPHPRMHHRRFVLAPLNDLAPNLLHPTLGTTIADLLQSVDEHPKVI
                     RWTPS"
     gene            2772..4457
                     /locus_tag="VFH91_02180"
     CDS             2772..4457
                     /locus_tag="VFH91_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861816.1"
                     /translation="MSQLLTLIWLKWKLLRNSLRSSKAIANQLASALGTLITLVIATG
                     VSLGLGITAYLITHSVEMAAVMQRTMAQQSRSAASAEFIFFSIFSFLYLMWATLPLSI
                     GGSKQFDAGRLLMYPISLGKLFAVDFISELTTLQSLFAVPAIIAIAIGAGLGTGNLVG
                     CLLAAIPAIVFGVALSKWLATTIGSLVRRKRARGETLLALIGAVAGLGGALAGQLGPI
                     LISHAESFRSLRWTPPGAAAFLMGGEGSKDPVTSAISFVLLMFYGGALIAGTFWIARR
                     SALGLGGSKKRARTELVANNTPYTGWEFPVISSELSAVVEKEIRYAMRNAQLRMMALM
                     PLILIIIRFANSRRFRGGLESGVSAASSTFKTYGSGLIVTGGVLYVFLILAGISCNLF
                     AFEEGGMRSFILSPIARRKILMGKNITVVLLAIIFSIALLIVNAIVFRDLTLPNILFA
                     ALSFVCFAALMSLMGNWFSIRFSKRMQYGKRLNVSGVAGLLLIPMILLLSIAPIGSVI
                     VGYLTRNVIYEYVTLAGLAAVSVGMYCLFIGVQGRSLQRRELEILEAVKEPDQ"
BASE COUNT         1078 a         1145 c         1160 g         1247 t
ORIGIN      
        1 aacgagtatt gcgcaggttc cgcactcgcc aagttcacaa ccatgttttg ttcctgtcag
       61 attgagatct tcgcgcaaaa cttcaagaag agtcttatgc ggagcgaacg caacttctac
      121 ttgctcgtca ttaagtgtga aggtgatatg agacttctgt ttcattgcga caaaaaatta
      181 gcgtctttgc agccccgcgg gtctattgct attgcattat tccccgcaca ggcacaaaga
      241 cgcaaagacc gagaggactt gcagcttaag cgttaagacc aactgcttct ttcttcttaa
      301 ccttattaag aattatgttg tcgatcagac gagtcttgcc aatgtaagcg gcaaccgcaa
      361 tcaagatggg acgctcatca agcttttcta tccgctcgag agtttctgcg tccgctacgg
      421 tgacatagtc aactctggct cgcggttcaa cctcgatcgt tgagcggatc aatgcttcta
      481 atctaccgcc gttacgttcg ccatctttat acgctgtttt tgcgcgaccc aaagactggt
      541 gtatcactgt tgccgcggtt tgctcctcag catttaagta aaggttccgt gacgagattg
      601 ccaatccaga atcttctcga acagtaggta aaactacgat ttcagtatca aaggcgaggt
      661 ccttaacgag tcgtctgatg atgagcgctt gctgtgcatc cttctgtccg aaaaatgcaa
      721 aatcaggtcg cgttgtattg agcaagatag tcacaatcgt cgcgacaccg cgaaaatgac
      781 ccggacggga cgcaccttca agttggtctg ccaggccttc gacgtttaca taagttgaga
      841 agcccctggg atagatctct tcgaccgccg gtgcaaagat gtaatcaacg ttgtaatcag
      901 tcagcagcgc cgtatccttg gttagatctc gaggatatcg agtgaagtct tcgtttggac
      961 caaattgagt tggattgaca aaaaccgaga cgatgacgac gtcgcacatg cgtcgcgctt
     1021 cacgaaccaa actcaggtgg ccttcatgta gcgcacccat tgtaggtact agaccgatag
     1081 ttctgtcttg cacgcggcgg attttgcgcg cgacagaact catgcgctga cgacggttga
     1141 ttatctccat gatctgagat gcggcctagg attctttcag atttttcgtg ggggtctgaa
     1201 tatcaagtgc ctcagcggca tcggcaggga gtgcatacga ctcggcatcg gagggataag
     1261 tgccgtttct aacgtcgtca gcataccgag taacagcgtc ggtcatcact tcacgtaaat
     1321 ttgcatacgg cctaacaaat cttgcttgct taccaaacga cagacccaac atgtcatgta
     1381 tcaccagaac ttgaatatca cagtgggagc ccgctccaat gccgatagtt ggaatcgata
     1441 gactctcagt aatcatttgc gctatttctc gggggaccac ttcgaggacg accgcaaatg
     1501 ccccggcatc ttccatagct tgcgcgtcat cgagtaactg acgggctgtc tctgctgtct
     1561 tgccttgcac ccgatatgcg ccgagctgat tgatcgattg tggtgtcaag ccaatatggc
     1621 ccatcacgct aatctcttcg tctaccagtc gttttaccag gtgtactcgc ttgtgaccac
     1681 cctcaagttt gattgcttct gctccgccct ccttaacaag tcggagagca tttctcaccg
     1741 catcgtcatc tccggtgtgg aaagtgccat aaggcatatc agcaactaac aacgcgcgtt
     1801 ggaccgcgcg acgaactgcc ttcagatgga tgagaatctc ctcgagtgtt acgggtacag
     1861 tatttccgta tcccaaaaca acattaccca gactgtctcc caccaaaatg atgtcgattc
     1921 cagcctcgtc tacgatcctt gccgtcgggt agtcgtaggc ggtcagacat accaatttct
     1981 cctcccgttg tttactggat cgcaatgacg gcgcgctgac tttttccgga cgttcaggtt
     2041 tcaaatatgg catatttcag ctctctttac actttggatg tttggacaat tgggttgcca
     2101 gagaaacgaa gaatcaggaa atttcgcggg cgtgataaca agtgtggcct cattatagcg
     2161 gcaaagggcg aacaggcaaa agtctcggca ctatgacggg gtccacctga tcactttggg
     2221 atgctcgtcc acgctttgaa gtagatcggc gattgtcgtg ccaagcgtcg ggtggagaag
     2281 attgggagct aaatcgttca agggtgcgag aacgaaacga cgatgatgca ttcgcggatg
     2341 aggcagcctc aaaaactcgg tgtctcgaat ttcactctta tataacagca aatccaggtc
     2401 aatcgtccga ggtccgtttg gtacatcccg tgtgcgcccg agtgcatatt caactcgtaa
     2461 aagcctggcc aacatctgtt ccggcggtgg cagggacttg cctcgtactt cggctacctg
     2521 attcaggaaa tacgattgag ccagatctcc gatgggctca gtctcgtaga taggagataa
     2581 gcgtatgatt tccagccccg cgtccaaaat gccgcggaca gcaaggagca agttaccggc
     2641 ccggtctccc aaatttgagc ctagaccaac atatgcatta ggaatcatcg gcagaggtct
     2701 gagtggcaac tgaaaagtcg cgcacattat tcccttgagt ggcggtcaaa tcaactatat
     2761 tgtcgtaaaa catgtctcaa ctcctgacac taatctggct taagtggaag ctgttgcgaa
     2821 actcgcttcg ttcctcaaag gcaatcgcaa atcagctcgc gagcgctctg ggtactttaa
     2881 taactttggt gattgctact ggtgtgtcgc tgggcctggg cattaccgca tatctgatta
     2941 cccattccgt cgaaatggct gcggtgatgc aaaggaccat ggcccaacaa agtcgttccg
     3001 ctgcatctgc cgagtttatc tttttttcga ttttctcctt cctctatctg atgtgggcca
     3061 cactcccgct tagcataggt ggcagcaaac agttcgacgc cggccgctta ctaatgtatc
     3121 caatcagttt ggggaagttg ttcgcagtcg acttcataag cgagctgaca actctgcaat
     3181 ccttgtttgc ggtccccgct attattgcga tcgcgatcgg tgctggatta ggcacaggta
     3241 atctcgtggg atgcctactc gctgccatac cggcgatcgt ttttggagta gctctctcaa
     3301 agtggcttgc aaccacgatt gggtcgttgg taaggcgaaa aagggcgcgc ggagagactc
     3361 tcttagcgtt aataggcgct gtcgccggac tggggggcgc tttagccggc cagcttggtc
     3421 caatactgat cagtcacgcg gagtcttttc gatcgttacg ctggacgccg cctggagccg
     3481 cggcattctt aatgggaggc gagggctcta aagacccggt aacgtctgcc attagttttg
     3541 tactactgat gttttatggc ggtgcgctca ttgccggtac tttttggatt gctcgacgtt
     3601 cagctcttgg attgggaggt tcaaagaaac gggccaggac tgaacttgta gctaataaca
     3661 caccctacac tggttgggag ttcccggtaa tttcgtctga gttatctgcc gtagtcgaaa
     3721 aagagatccg ttacgccatg cgcaacgctc agttgagaat gatggcgctg atgccgttga
     3781 ttctcattat tattcgtttc gcaaactcac gtcgatttcg cggaggtctc gaatctggag
     3841 tttctgcagc gtcgagcaca ttcaagactt atggttctgg actaatcgtg acaggtggag
     3901 tgctttacgt cttcctgata cttgccggga tttcctgtaa cctttttgca ttcgaagaag
     3961 gaggtatgcg ttcgttcatc ttatcgccaa tcgccagacg caaaattttg atgggcaaga
     4021 acatcacggt agtcttgctg gcgataatct tttccatcgc tcttttgata gtcaacgcaa
     4081 tagtttttag agacctgacg ctgcctaata ttttgttcgc cgcactaagt tttgtttgct
     4141 ttgccgctct gatgtcgttg atggggaact ggttttcaat tcgtttctcg aaacgaatgc
     4201 aatatggaaa gcgattgaac gtttccggag tagccggtct gttattaatt ccaatgattt
     4261 tgcttctatc gattgctccc attggttcgg tgatagtcgg ataccttacg cgcaacgtca
     4321 tctacgagta tgttacactg gccggacttg cggccgtctc agtcggaatg tactgcctgt
     4381 ttattggagt tcagggtaga tcgttacagc gccgcgagct cgaaatactg gaggctgtta
     4441 aggagcctga tcagtaacgt tttacggatg acggttttca caattgaccg tcgttcattt
     4501 ttaacaagtc ggtttgaaaa ccagtgatgc ttgattttgc atccgggcgg gtttcgggag
     4561 ccccgtcatt acacaaatgg ctcgaagaag atttcgctct aaccaacgac acgaaccctc
     4621 gaccgaacgg
//
LOCUS       DASSFP010000104        12860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_916141, whole genome shotgun sequence.
ACCESSION   DASSFP010000104 DASSFP010000000
VERSION     DASSFP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916141"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..2869
                     /locus_tag="VFH91_02185"
     CDS             350..2869
                     /locus_tag="VFH91_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET6861817.1"
                     /translation="MKSNILFVLICLLISPSTSEAQDLSRLIKIGSATEFAKQSDGLT
                     VNCADGSQVQLKVLAPDLIRVRAAFAKTLDSADHSWAIAKESWPPVQWTVAESTAAIT
                     LKTNELVVNINRSPLLIEFRDLRTGEVINADQQPMMYDRDRTLNNMMFDPAAGMFIVA
                     AKKLGLNEHFYGLGEKAAKLDKRRGSFVNWNSDTPGYSEGKDPIYQTIPFFIGLERGR
                     SYGIFFDNSYRSHFNFGFNSQERIWFGAEGGEMNYYFFYGPSMKKVVSRYADLTGHMP
                     LPPRWTLGNQQSRWSYYPDSLVEQVVAEYRRRDLPLDAIHLDIDYMDQYRVFTWNRDR
                     FPDPKGLIDRLSKQGVKVVTIVDPGVKHQGKSSSSPGTGYYVLDQGLEKNYFQRRRNG
                     DLFIPKVWPGASVFVDYTNPEARRWWGDLHRAYTDQGVAGIWNDMNEPADFVDQSGKN
                     QLDVISYDEGAKTSHAKNRNVFALLMARATYEGLQRLQPDKRPYVITRAAYAGIQRYS
                     TMWTGDTNSTWDGLALSVSMFATLGLSGEPFVGADVGGFMGRANGELLARAYQVSFLA
                     PFCRNHKTIDSYDQEPWRFGKYYEDVIRKYLKLRYRLLPFLYTTLEESHRTGLPLFRP
                     LVLNYPEDESTYNLDDQFMIGDDLLVAPVLKPDVTRRLVYLPKGIWYDYWTREKYQGD
                     RMIDVKAPLDTVPMFVRGGAILPFGPEMNYVGEKPLEPTFVIYPDEQGAAQTSLYEDD
                     GVSPAYLNGTYRRTSLGVSTTGLEVLIRVTAAEGSYQTSRRTMSFVLPFRANNFRVVV
                     DGRALDRAAVGQLGWSIGPDGLTIRTTDDGQAHAIQVNQLP"
     gene            3256..3426
                     /locus_tag="VFH91_02190"
     CDS             3256..3426
                     /locus_tag="VFH91_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861818.1"
                     /translation="MKLKLQAAEAGGVGDGLVRWARWKLIGHATEVGGVRRISNEFLD
                     TVASRLLLSGLR"
     gene            3578..3805
                     /locus_tag="VFH91_02195"
     CDS             3578..3805
                     /locus_tag="VFH91_02195"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HET6861819.1"
                     /translation="MKRTLLVVAVVALYLLHQDVWFWRNGRLVFGFIPIGLFYHACYS
                     VAASLVMWLLVTYAWPAHLEQEVEEEEGHKH"
     gene            3802..5421
                     /locus_tag="VFH91_02200"
     CDS             3802..5421
                     /locus_tag="VFH91_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET6861820.1"
                     /translation="MIPAAVVFLYLAIVLYIGIFAFRRAKGKEEAEDYFLASRSLGPF
                     VFLFSLFGTNMTAFAILGSSGHAFTNGIVTFGLMASSSGFVIPLTIFFIGTRVWALGK
                     KYGFITPVQMFRDRWECGHIGTVIFGVQSVLLVPYIIIGIMGGGTTLYAISGGAVPFW
                     FGGAIVAMVVMSYVFFGGMRGTAWVNTFQTVLFLLFGAIALIVIGVGMGGFSTAAHAI
                     QASPMATLLTRERISTFYFFSYTFIPLSAIAFPHILIFCLTARKMSQFKKTVIFYPLC
                     IMAIWLPCVFLGVMANRVTDVPQIRAKQEARRVLATQGKNMEPEQRDELRDAAAGDDV
                     IMLLLQRYAPLWLAGLLGAGIMAAVMASDSQILALSTMFTEDVFAYYGGKKRFGEAVQ
                     VQTGRVFIVLITLFAYAVALRAPETIFELAIQYAFSGFAALSPLLVAALFWKGSTKWG
                     ALAVTVWTAITVVAVAIFQAIVPAPAPGPSTVVWSLGGAEVLSRTPGGTAVFGFMPVV
                     PMVIVSVLLMIVVSMATAKPRESTLARYFGG"
     gene            5426..5893
                     /locus_tag="VFH91_02205"
     CDS             5426..5893
                     /locus_tag="VFH91_02205"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6861821.1"
                     /translation="MKRSIWLMAATLLAWTTVVGQEVSSTQKPENPKRRVAKTASPPN
                     GLFSPAIISGDLVFTSGQIGIDPKTGQLAEGLEGQLEQVFKNLTAALEAAGSSKEHIL
                     KATVFLTDMSDYNAMNDLYRKHFKADPPARTTVQVVKLPRDARIEIEAVAVLK"
     gene            complement(5992..7119)
                     /locus_tag="VFH91_02210"
     CDS             complement(5992..7119)
                     /locus_tag="VFH91_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6861822.1"
                     /translation="MRLLNRRSLIILACSILLGIICIPVTAQDTPADNWAQFRGNPRL
                     TGVSGSTVPDSLKQIWTYEAGDSIESSAAIVGGTVLIGSQKGELVSLSLDNGQVYWKY
                     STGAPIGESSPAYGNGVVYIGDLSGVLHAVNAPDGKRLWTFKSGNEIKSSPVVVGDRV
                     LIGSYDENLYCLSTRNGSVLWKVKTNGPVHSTPSIMNGMAFIAGCDEMFRAIRISDGK
                     EMFTVSSGSYTGASPALRLGNAYYGTFDNEVLGVSLTQHKIMWRYQHPTRKFPFYSSA
                     AVTQDRVVVGGRDKMVHGLNLAGKNVWTFATRARVESSPAIAGGKVFVGSNDGRFYVL
                     SLSNGNKLWEFTAGAPLSASPAIANGRIVIGSQDGKLYCFG"
     gene            complement(7107..8264)
                     /locus_tag="VFH91_02215"
     CDS             complement(7107..8264)
                     /locus_tag="VFH91_02215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET6861823.1"
                     /translation="MRVLNSFDYKPKTRVVFGVGSIARLGELATELGFRRTLLVADHG
                     LVASGHVDEALEPLKAARVEVVRFHDFEANPDSLMIEAGTIFARAEKIDSIIGLGGGS
                     SMDCAKGINFLITNGGRMADYRGYGKTSAPLLPMIGIPTTSGTGSEAQSFALISDSET
                     HVKMACGDPGAAFRVALLDPALTVSQPRIITATSGFDALAHAVETFVTTKRNAVSDLF
                     SREAWRLLESNYESVLANPGDLEARGAMQLGAYYAGLAIENSMLGATHACANPLTARY
                     GTPHGEAIAMLLPTVVRWNESAAADRYRELLHLSSAANKTRQVSATEALAQRLEQLAA
                     FGGLHTRLSNAGISDSDFPALAADAAEQWTGTFNPRTFNLEGALEVYRCAY"
     gene            complement(8432..9928)
                     /locus_tag="VFH91_02220"
     CDS             complement(8432..9928)
                     /locus_tag="VFH91_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6861824.1"
                     /translation="MRNTAVASRNKAAKVIILVAIIGAALSLNTFHTSASDPGSGDWP
                     MWGGTPDRNMVSNMKGLPTTWDVKTKKNVKWVAQLGSQSYGNVVVSGGMVFVGTNNEG
                     LKDPKITGDKGVLMAFRESDGQFMWQMVHDKLAAGRVNDWPYQGVASSPLVEGDRIYY
                     VSNRAELVCLDTQGFRDKENDGPVTDEALTGETNADVVWKFDMIEEVGSLPHNLANSS
                     PVSYGDLIYVSTSNGQDESHVNIPSPKAPSIIAVNKKTGKLVWEDNSVFDRILHGQWS
                     SPTVGTIGGVVQVVHGQGDGWVRGYEATTGKKLWEFDTNPKDSVWPKTRNELISTPVV
                     YKDRVYISNGQDPEHGEGVGHMYCIDATKRGDITKTGLIWHYDKIRRSISTPAIKDDI
                     VYQADFSGFLHALDANTGQPFWTHDLFAAVWSSPMLIDGKIYIGDEDGDVLIMQEGKT
                     EKVISEINMGSSVYSTVVPANGMLFITNRNQLFALQDTAQPKATTARN"
     gene            complement(9925..12108)
                     /locus_tag="VFH91_02225"
     CDS             complement(9925..12108)
                     /locus_tag="VFH91_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6861825.1"
                     /translation="MSSFLSIILNVFLAATVLVTASDWADWRGPARDGISVEKNLPEK
                     WSPSGDNLLWKAPYGGRSSPIVMGDRVYVQNSAGKGADMQERVMCFNADTGKLLWEHR
                     FNVYLSDVPPHRVGWASPVGDPATGNVYVFGVGGNLIGLNRDGKVLWERSLGEDFGLL
                     TTHGGRTVSPIIDGDLVIVSGVTFMWGLHGRGAHRFMAFDKKTGETMWVSAPGGRPYD
                     TTYAPPIIANINGTRLLIQGASDGVVHAIKVQTGEPVWKYEISKRGLNTGAVVYGNTA
                     ILTHSEENLSSNEMGMMVAVDASAKGDIKKENIKWSTYGWQGGFSSPVLDGERLYQID
                     NGANIAAFDINTGKQLWLKNLGTIQKASPVLADGKLYVGTENGKFFILRPTATGCDIL
                     DEHQLGTEAQPETIIGSAAVANGRIYFVSDSNVYCIGKKGTNRSHNTATETTKAVADT
                     TPAFLQVTPTELILKPGDKVTFHARTFDAHGNLIGEQTADAWSLDQLKGTIDKGQFVA
                     SSDAVVQAGLVKATIGGLSGSASVRVFPPLPWSETFDSLAVNSLPLGWVNTTLKFAVR
                     DVNNNKVLVKLTEGSSLLSRARAYMGPSDLSNYTIESDVLATQKRRQQGDAGVIAQRY
                     VLTLYGNSQMLHIEPWQPEVKRTVNIPYAWKPDTWYRLKLEVENLPDGKTRARGKVWP
                     VGETEPGAWMIERIDPIPNRQGAPGVFGNGLAELYFDNIKVYANK"
     gene            12439..>12860
                     /locus_tag="VFH91_02230"
     CDS             12439..>12860
                     /locus_tag="VFH91_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6861826.1"
                     /translation="MQFLKVAFITLLSVNLAVLVQDPKQQLNDQLWEAVRKGDAASVT
                     ALLDKGADVNAKFRYGTTALFKAAERGHTEIVKILLARGADASVKDTFYQATAMTWAL
                     DGKHVEVVKLLLQKVPDDADDVLTTAVSESNQQLVQVAL"
BASE COUNT         2996 a         3251 c         3331 g         3282 t
ORIGIN      
        1 aacttcccat gtggtcaaat ttcaattatt cagcggatgg ggagatcgct tgttggtgaa
       61 tggtctaatg ggtgtggtcc taactggcag aggagtccac gccgacactt atttgacatc
      121 tgtaacggtt cggggattgg atttgatatt acagcaaaaa caaaaaactt caacatgaat
      181 ttaatagttg gctttgatag gcttgcgctc caaaaacctg gtgcttacgg ataatttgcg
      241 ttcacctttt gtaaagcacc gaaactttcg caagctttag cacataaatt tggaccattt
      301 agtcacgaag tgttcggaac cgtgaccgtt tcgcaatcga gccgcgccaa tgaagagcaa
      361 catactattt gtactcatct gtctactaat ttcgccttca acttctgagg cgcaggacct
      421 cagtcgcctc attaaaattg gttcggcgac tgagtttgcc aaacaatcag atggcctaac
      481 cgtcaattgt gcagacggct cacaggtgca gcttaaggta ctggccccgg atctaattag
      541 agtgagagca gcgtttgcga agacgttgga cagtgccgat cattcctggg ccatcgccaa
      601 agagagttgg ccacctgttc agtggaccgt ggcagagtcg acggccgcaa taactctaaa
      661 gacgaacgag ttggtagtta acatcaatcg ttctccgtta ttgattgagt ttcgagatct
      721 cagaacgggc gaggttatta acgcggacca acagccaatg atgtacgacc gcgatcggac
      781 actcaacaat atgatgttcg atccagccgc tggaatgttt attgtcgccg caaaaaagct
      841 cggtcttaac gaacacttct acggtcttgg tgagaaagcc gccaagctcg ataagcgacg
      901 cggttctttt gttaattgga attccgacac gccaggctac tccgaaggca aggaccctat
      961 ctatcaaacc attccgtttt ttattggact cgaaagagga cgcagttacg gaatcttttt
     1021 cgacaactcg tatcgcagcc acttcaactt tggcttcaac tcacaagaga gaatctggtt
     1081 tggcgccgaa ggtggtgaga tgaactatta cttcttctat ggaccatcga tgaagaaagt
     1141 tgtcagccgt tacgctgatc tgacaggcca catgccgttg ccgccaaggt ggacactcgg
     1201 aaatcaacag agccgttgga gctattatcc ggattcgctg gttgagcaag ttgttgctga
     1261 ataccgccgg cgcgatttgc cgctcgacgc gatccacctc gatatcgatt acatggatca
     1321 atatcgtgtt ttcacctgga atagagatcg gttccccgat cctaagggcc taattgatag
     1381 attgagcaaa caaggcgtca aggtagtgac gattgtcgat ccgggcgtta agcatcaggg
     1441 gaaatcgtct tcgtccccgg gcaccggata ttacgtactc gatcaaggtc ttgagaaaaa
     1501 ctatttccaa cgacggagaa atggcgatct cttcataccg aaagtgtggc ccggagcaag
     1561 cgtgttcgtt gattacacaa acccggaagc acggcgttgg tggggtgatt tacatcgtgc
     1621 ttatacggac cagggggtcg ccggaatctg gaacgacatg aacgagccgg ctgactttgt
     1681 cgatcagtcg ggtaagaacc agctcgatgt aatcagttac gatgaaggcg cgaaaacctc
     1741 tcacgctaaa aaccgaaatg tgtttgcctt gttgatggcc agagcaactt acgaaggtct
     1801 acaacggttg cagcctgaca agcgtccata tgtgatcacc cgagcagctt acgcgggaat
     1861 tcaacggtac tcgacaatgt ggaccggcga cacaaacagt acctgggacg gcctggcttt
     1921 atcagtgtcg atgttcgcta ctcttgggct ttccggcgag ccttttgtgg gcgcggatgt
     1981 tggcggattc atgggaagag cgaacggtga actcctggcg cgcgcttacc aggtgagttt
     2041 tcttgcgccg ttttgccgga accacaagac gattgatagt tacgatcagg aaccctggcg
     2101 gttcggcaag tactatgaag acgtaatccg caaatacctt aagctgcgat atcgtctgct
     2161 gccttttctc tacacgactt tagaagagtc gcaccggaca ggactgccgt tgtttagacc
     2221 actggtcctc aattatccgg aagatgaaag cacctacaat ctcgatgacc aattcatgat
     2281 tggcgatgat ttgctggtgg ctccggttct gaaacctgat gtaacgcggc ggttggtcta
     2341 tctgccaaaa ggcatctggt acgactactg gacacgcgag aaatatcagg gagacagaat
     2401 gatcgacgtg aaggcgccac tcgatactgt cccaatgttt gtgcggggcg gcgcgattct
     2461 gccctttgga ccagagatga attatgttgg cgaaaaaccg ctcgaaccaa cgtttgtaat
     2521 ctatccagac gagcagggtg ctgcacaaac gagcctttat gaagacgacg gagtgagtcc
     2581 cgcatatctg aacggaactt atcggcgtac gtcgctcggc gtttcgacga ctggtttgga
     2641 ggtcttgatt cgggtcactg ccgctgaggg tagttatcag acgagtcggc ggacaatgag
     2701 ttttgtcctc ccgtttcgag caaacaattt ccgagtcgtt gttgatggcc gcgctttgga
     2761 tcgagcagcc gtaggtcagt taggatggtc catcgggccg gatggtctaa ctattcggac
     2821 cactgacgat ggccaggctc acgcgatcca ggtaaatcag ctaccttgac gcgtgtgcta
     2881 aatgccaccg gctttagccg cgtggtactt agttttcacc tagcccaata gtcatgtttg
     2941 actatttcat accaccggct tcagccgcgt ggtacttgag tttccaccta acccaataat
     3001 cgtgctttga ctccttcata ccaccggctt cagccgcgtg gtacttgagt tttaagctgg
     3061 aaatcaactc aagaatcgac ttcacaaact cgcgaatgat gtttgaatga ggttaggttg
     3121 aaattgaacg gaccacacgg ctgaagccgg tggtgtcccg taacatctcg accgcggggc
     3181 taggatgaaa ttgaacggac cacgcggctg aagccggtgg tgtcccccca aaacatcacg
     3241 aaccacgggg ctaggatgaa gttgaaactc caggcggctg aagccggtgg tgtcggcgat
     3301 ggtttggtgc gttgggctag gtggaaattg atcggccacg cgactgaagt cggtggtgtc
     3361 cggcgcattt ccaatgaatt tcttgacacc gtcgcgagcc gtttgctact ttcgggcctc
     3421 cgttaagtca aagagaaaga gtcagttgag ctattcgtgc taactaacgg tgaccgcgcg
     3481 cctttttcga acgtgctgtc gtacttctcc tgactcgttc cactcttcag caatttagat
     3541 cgcagcttaa aatttcgtcc gtaactagca caacaatatg aaacgaaccc tccttgttgt
     3601 agcggtcgtt gctctctatc tcctgcacca ggatgtctgg ttctggagaa atggcagact
     3661 cgtttttggc tttataccca ttggactctt ttatcacgca tgttattccg tggccgcctc
     3721 tctggtgatg tggttgttgg ttacatatgc gtggcctgcc cacttagaac aagaagttga
     3781 agaagaggag ggccacaaac attgattccg gcagccgtag tatttctgta tctcgccatt
     3841 gttctgtaca tcggaatctt tgcctttcgg cgtgcgaaag gtaaggaaga agccgaggac
     3901 tactttctag caagccgttc gcttggtcca tttgtctttc ttttctctct gttcgggacc
     3961 aacatgaccg ctttcgctat ccttggctca tcggggcatg cttttacaaa cggtattgtc
     4021 acgttcggat tgatggcctc gtcttcgggt tttgtcatcc cgctaacaat cttctttatt
     4081 ggtacgcgcg tttgggcgct cgggaagaaa tatgggttta tcaccccagt ccagatgttt
     4141 cgagatcgtt gggaatgcgg gcatatcggc acagtcattt tcggcgtgca atctgtatta
     4201 ctcgtgcctt acatcatcat cggaataatg ggaggtggta ccactcttta tgcgattagc
     4261 ggtggcgctg tgcctttttg gttcggcggc gcaattgtcg caatggtggt gatgagttat
     4321 gtcttcttcg gtggcatgcg cggcacagcg tgggtcaata cattccagac ggtgctgttt
     4381 cttttgttcg gcgccatcgc tcttatcgta atcggggtag gaatgggagg atttagcact
     4441 gcagcacatg caatccaagc ctctccaatg gcgaccctgc tgacccgaga gcggatctca
     4501 accttttact tcttcagcta tacatttatc cctttgtcag ccatcgcttt tccacacatt
     4561 ctgatctttt gtctaacagc tcgaaagatg agccagttta agaagaccgt catcttctat
     4621 ccgctctgca tcatggccat ctggttgcca tgcgtatttc tcggcgtcat ggcgaatcgc
     4681 gttaccgatg ttccgcagat tcgtgcgaaa caagaagccc gaagagtact ggcaacgcaa
     4741 ggcaaaaaca tggagccgga gcagcgcgac gagttgcgag atgccgcggc cggcgatgat
     4801 gtcatcatgt tgttgctaca acgatatgcg cctctctggc tcgctggatt gctgggagca
     4861 ggaatcatgg cggcggtaat ggcaagcgac tctcaaatac tggcgctttc gaccatgttc
     4921 acggaggatg tctttgctta ttacggaggt aagaaacgtt tcggcgaggc tgttcaagta
     4981 caaaccggac gtgtgttcat cgtcctgatc accctctttg cttacgccgt ggcgctgcgc
     5041 gcacctgaga caatttttga gttggcgatt caatacgcat tttcaggttt cgcagctctc
     5101 tctccattat tggtcgcagc gctgttttgg aagggaagta cgaaatgggg agcgctggct
     5161 gtgactgttt ggacggccat cactgtggtg gctgttgcga tcttccaggc tattgttcca
     5221 gcgccggctc ctggtccatc cacagttgtc tggtcgctgg gtggcgcgga agtcttgtcg
     5281 cgaactccgg gcggaacggc ggtctttggt tttatgccag ttgtgccgat ggtgatagtt
     5341 tcagtcttgt tgatgattgt ggtgtcgatg gcaaccgcca agcctcgtga atcaaccctc
     5401 gcaagatatt ttggaggata aaaacatgaa acgttcaatt tggttaatgg ctgcgaccct
     5461 gcttgcctgg accacagttg ttgggcagga agtttcttcc actcagaagc cggaaaatcc
     5521 aaaacggcga gtcgcgaaaa cagcttctcc tccgaacggc ctgttttcac ctgcgataat
     5581 ttcgggcgat ctggtgttta cctcaggtca aattggaatc gatccaaaaa ccggacagtt
     5641 agctgaagga cttgaaggtc aactggaaca ggtattcaaa aatcttacgg cggctttaga
     5701 agcggctgga agtagcaaag agcacatctt gaaagcgact gtctttctca ccgacatgag
     5761 cgactacaac gcgatgaatg acctttatcg taaacacttc aaagctgatc cgcctgcgcg
     5821 gacaacggtc caggttgtaa aattgccgag agatgcccga attgagattg aagccgtcgc
     5881 agtcctgaag tgaccttgga gtgcgtcggc ccggcggcgc tttgttataa gagagtctcg
     5941 acaccctggg agttttcgaa aaagcgccgc cgggccggcg cactccaaag ctcagccaaa
     6001 gcagtagagt ttgccgtcct gcgagccgat cacgatgcga ccattcgcga tcgcaggtga
     6061 agcagataaa ggcgctccgg ccgtaaattc ccagagcttg tttccgttgc tcaaactcaa
     6121 tacgtagaac ctcccgtcgt ttgaaccgac aaataccttt ccccctgcga tagccggtga
     6181 tgactccact cgcgcacgcg tcgcaaacgt ccaaacattc tttccagcga gattaagtcc
     6241 gtggaccatc ttgtcgcggc cacccacaac gactcgatct tgagtcacgg ctgctgaaga
     6301 gtagaacgga aactttcgtg ttggatgttg atagcgccac atgatcttgt gttgcgtcag
     6361 acttacccct aacacttcat tatcgaacgt tccgtaataa gcatttccga gtcttaaggc
     6421 aggcgaagca ccagtgtaag aaccagaaga gacggtaaac atctctttgc catctgaaat
     6481 gcgaattgct cgaaacattt cgtcgcaacc ggcgataaac gccatgccat tcataatgct
     6541 cggagttgag tggacggggc cattggtttt gactttccaa aggaccgaac cattgcgcgt
     6601 cgaaaggcag tacaagtttt cgtcatatga accgataaga acccgatctc cgacaaccac
     6661 gggtgacgat ttgatctcgt tgccggattt aaaggtccat agccgctttc cgtcaggtgc
     6721 attaactgcg tgtaatactc ccgacaagtc gccgatgtag accacaccgt tgccgtaagc
     6781 tggagacgat tcgccgattg gcgcgccggt ggaatatttc cagtagacct gtccgttatc
     6841 gaggcttaag gagaccagtt ctcctttttg cgatccgatc agaacggttc cgcctactat
     6901 tgcggcagac gactctatcg aatctccggc ttcataggtc cagatctgtt taagggaatc
     6961 aggaacagtt gaacccgaga ccccggttag tcgtggatta cctcggaact gtgcccagtt
     7021 atcagccgga gtatcttgtg cagttactgg aatgcaaatg atgcccagca atatggaaca
     7081 ggcaagaatt attaaacttc tgcgattcaa taagcgcatc ggtaaacctc caacgcaccc
     7141 tcgagattga atgtcctggg attaaaggtg ccggtccatt gttcggcggc atcggcagcc
     7201 agagctggaa agtctgaatc gctgattcca gcattgctaa ggcgagtatg caaacctccg
     7261 aaagcagcaa gttgttcaag tcgttgggcc aaagcttcgg tagcactgac ctgccgagtc
     7321 ttattggccg cgctggagag gtgcagcagt tccctgtaac gatcagcggc cgcggattca
     7381 ttccagcgaa ctacagttgg cagtaacatt gcaatcgctt caccgtgagg cgtgccatag
     7441 cgagcggtta gcggattcgc acaagcatgc gtggcgccca gcatggaatt ttcaatcgcg
     7501 agcccggcgt agtaagcgcc cagctgcatt gctccgcgag cttcaagatc gcctggattc
     7561 gcgagaactg attcgtaatt cgactcgagc agacgccatg cctctcttga gaaaagatca
     7621 gatactgcgt tgcgctttgt ggttacgaat gtctcaaccg catgggccag agcatcgaat
     7681 ccggaagtcg ccgtgatgat acgaggttga gaaaccgtca aggcagggtc gagcaaagcc
     7741 actctaaaag cagctccagg atctccgcag gccattttga cgtgcgtttc ggagtcggaa
     7801 attagtgcaa agctctgcgc ttcacttcct gtgccagacg ttgtcggaat gccgatcatc
     7861 ggtagtaacg gagcgctggt cttaccgtac cctcgataat cagccattcg tccgccatta
     7921 gtaatcagaa agttaatacc tttagcgcag tccatcgaac tgccgccacc aagcccgata
     7981 atcgaatcga tcttttccgc gcgcgcgaag atggtccctg cttcaatcat caacgaatca
     8041 gggttagctt cgaaatcgtg aaaccgcacg acttcaactc gagctgcctt cagcggttcg
     8101 agcgcttcgt cgacgtgtcc gcttgcaaca agaccatgat ctgcaacgag cagggttcta
     8161 cgaaacccga gctctgtcgc cagttcaccg agtcgagcaa tcgagccgac gccgaatact
     8221 actcgcgttt ttggtttgta atcgaaagag ttgagaactc gcatcttcag ttcgactgtc
     8281 ctccgcgttg accaactgcg aagaccgaat cagtcgtgcg taagtttacg agcccgtctg
     8341 taactagtat tcgaaggagg acaaccggct tcatggacgc tcctcaaaag tgtcagatac
     8401 aggatgatta aatggggagc tcaggaaaac tctagttcct cgcagttgtg gccttcggtt
     8461 gagccgtgtc ttgtagagca aagagttgat tgcggtttgt tatgaacaac attccgttag
     8521 ccggaaccac agtcgagtaa accgagctac ccatattgat ctcagaaatg accttctcgg
     8581 tttttccttc ttgcatgatg agaacgtcgc catcttcgtc accaatatag atcttgccgt
     8641 cgattagcat cggtgagctc cagactgcgg caaacaaatc gtgcgtccaa aacggttgac
     8701 cggtgttggc atccaatgcg tgcaagaaac cgctaaagtc ggcctgataa acgatgtcat
     8761 ctttaattgc cggagtcgag atcgagcgtc gaattttatc gtagtgccag atcagaccgg
     8821 tcttggtgat atcgccgcgc tttgttgcgt cgatgcaata catgtggccg acgccttcac
     8881 cgtgctcggg atcctgtcca ttcgagatgt agacgcgatc tttgtagacg acgggtgtgc
     8941 taatcagttc gtttctggtc ttgggccaaa cagaatcctt gggattagta tcgaactccc
     9001 aaagcttctt tccagtggtt gcttcatacc cgcgaaccca gccatcgccc tgtccatgta
     9061 cgacctggac cacaccccca atggtgccaa cggttggaga agaccactgg ccgtgcaaaa
     9121 tacgatcaaa aactgaattg tcttcccata ccaactttcc agtcttctta ttgaccgcga
     9181 tgattgaagg cgctttcggc gacgggatat tgacgtggct ttcatcctgg ccatttgatg
     9241 tgctgacgta gatcaggtcg ccataggaaa caggagaaga attcgcgagg ttatgtggca
     9301 gagatccgac ctcttcgatc atgtcgaact tccacacgac atcggcgttc gtttcaccgg
     9361 ttaaggcttc gtcggttact ggtccatcgt tctccttatc gcgaaaaccc tgcgtgtcga
     9421 gacagacgag ttctgcacga ttggatacgt agtaaatccg atcgccttcg accagcggag
     9481 acgaggcaac accctggtag ggccaatcat tgacgcgtcc ggcagcaagt ttgtcgtgga
     9541 ccatctgcca cataaactga ccgtctgact cgcggaatgc catcaaaacg cctttgtcgc
     9601 cggtaatttt tgggtctttt agtccttcgt tattggtgcc gacgaagacc atgccacccg
     9661 acacgacaac gttaccgtaa ctttgtgagc cgagttgggc cacccatttc acgtttttct
     9721 ttgtctttac gtcccaggtg gttggcagtc ccttcatgtt ggagaccatg ttccggtcag
     9781 gtgtgccgcc ccacatgggc cagtcgccag agccagggtc agaggcagaa gtgtgaaacg
     9841 tattaagcga taacgctgcg ccaatgatgg caaccaggat gattactttt gctgctttgt
     9901 tccgactcgc cactgctgtg tttctcattt gtttgcgtac acctttatgt tgtcgaaata
     9961 gagttcagcc aggccattcc cgaaaacacc gggagcgccc tgacgatttg gaatcggatc
    10021 gattcgttcg atcatccatg cacctggctc agtttcaccg actggccaaa cttttccgcg
    10081 agcccgggtc ttgccatcgg gcaaattctc gacttcaagt tttagccggt accatgtatc
    10141 cggtttccat gcgtagggaa tattgactgt acgtttaact tcgggttgcc agggttcgat
    10201 gtggagcatc tggctattgc cgtaaagcgt cagtacatac ctttgggcga taacgcctgc
    10261 atcaccctgc tgccttcttt tttgcgtcgc taacacatcc gattctattg tgtagttcga
    10321 aaggtcggac ggtcccatgt acgctcgcgc acgactcagc agggaggagc cttcagtcaa
    10381 cttcactaaa actttattgt tgttgacatc acggacggca aatttcagcg ttgtattcac
    10441 ccagccgagt ggaaggctat tcactgcaag gctgtcgaat gtttcgctcc agggcaatgg
    10501 cggaaaaact cgaacgcttg ccgagccgga tagtccgccg atggttgctt tcacaagccc
    10561 tgcctggacc acagcgtctg atgaagccac gaactggcct ttatctattg ttccttttaa
    10621 ttgatccagc gaccaggcat cagctgtttg ttcgccgatc aggtttccat gagcatcgaa
    10681 ggttcgggcg tgaaacgtta ctttgtcgcc cggcttgagt attaattcag taggtgtcac
    10741 ttgcaagaaa gccggtgtcg tatccgctac tgctttggtc gtttcggtag cagtgttgtg
    10801 gctccggttc gtgcctttct ttccaatgca ataaacgttc gagtcgctga cgaagtagat
    10861 acgtccatta gcgactgcgg cagagccgat gattgtctcc ggctgtgctt cggtaccaag
    10921 ctgatgttcg tccaggatgt cgcatccggt tgcggttgga cggagaatga agaatttccc
    10981 gttctcagtg ccgacataaa gttttccgtc agccagtacc ggtgacgcct tttgaatggt
    11041 gccaagattc ttcagccaga gttgcttgcc ggtgtttata tcaaaggcag cgatgttggc
    11101 tccgttgtcg atctggtaca gccgttctcc atcgagtacc ggggaagaga atccgccttg
    11161 ccacccgtaa gtgctccact taatgttttc tttcttaata tcgcctttag cgcttgcatc
    11221 gacggcaacc atcattccca tttcgttcga actgagattc tcttcactgt gagtgagtat
    11281 tgcagtgttt ccataaacca ccgcaccggt gttcagtccg cgtttgctaa tctcgtattt
    11341 ccaaacgggc tcacctgttt gtactttgat ggcatggacc actccatcgc tggctccctg
    11401 gattaacagc ctggtcccgt tgatgtttgc gatgatcggt ggtgcatacg tcgtatcgta
    11461 ggggcgtcct cccggtgcgc tgacccacat ggtctctccg gttttcttat cgaaggccat
    11521 gaatcgatga gcaccacgac cgtgtaggcc ccacatgaaa gtgacgccac ttacgatcac
    11581 cagatcgcca tctattattg gcgaaaccgt tctgccgccg tgggtcgtca gcagtccaaa
    11641 gtcttccccg agcgatcgtt cccagagcac tttgccgtct cgatttaaac cgatcaggtt
    11701 tccacccaca ccgaagacgt agacgttgcc tgttgcggga tcgccaacag gtgaagccca
    11761 tccaactcga tgcggcggta cgtcgctcag atagacattg aaccggtgtt cccataagag
    11821 ttttccggtg tccgcgttaa agcacataac gcgttcttgc atatctgcgc cttttccggc
    11881 ggagttctgc acgtaaacac gatcgcccat cacaatcggt gaagaacgac cgccatacgg
    11941 agccttccag agcaggttat cgcctgatgg agaccatttt tcaggcaggt tcttttcgac
    12001 ggagatgccg tcgcgagcgg gaccgcgcca atcggcccag tcgctggcag tcactagaac
    12061 tgtggcagca agaaaaacat tcagaattat ggataagaag ctactcatat tgaaggccca
    12121 atgtttaatg aaggagttgc gtaatccagt gcgcttttgc aaccttaagg atgtagaata
    12181 cgtaagccca gttgcagtcg ctacggtttc ttttcgacca aacttgttcg cagtgcaggg
    12241 atataatctt tcccttctcg aagttgagtc aagttgcgtc gcttgaaaag ctcaagttcc
    12301 gaaaagaggc gttttcgagt ctcagtagct catttcacaa gaatttagtt ccgtcgacgt
    12361 ctttatatca gatactacga gcgatcagtc ctaatagttt aaatcgcaat tgctaaaaaa
    12421 cacgcggagg agattgagat gcaattcctc aaagtggcgt tcataacctt gctcagcgtg
    12481 aacttggctg tccttgtcca agatccaaag cagcaactca acgatcagtt atgggaggcc
    12541 gtaaggaagg gagatgcggc atccgtcact gccttgttgg ataaaggcgc cgatgtcaac
    12601 gccaaatttc ggtacggtac gacggcacta ttcaaagcgg ctgaacgagg ccatacagag
    12661 atagtcaaga ttcttctggc cagaggggcc gacgcttccg ttaaggatac tttctatcag
    12721 gcaactgcga tgacatgggc cctcgatggg aaacatgtcg aagttgtgaa gctgttgctg
    12781 caaaaagttc cggacgacgc agatgatgtc ttaacgacag ccgttagcga aagtaatcag
    12841 cagttggtcc aggtcgccct
//
LOCUS       DASSFP010000105         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_916230, whole genome shotgun sequence.
ACCESSION   DASSFP010000105 DASSFP010000000
VERSION     DASSFP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916230"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2623)
                     /locus_tag="VFH91_02235"
     CDS             complement(<1..2623)
                     /locus_tag="VFH91_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6861827.1"
                     /translation="MTPERFQLISQIYHEALKRPEPDRESFLEQRCGPDEELTSEVRY
                     LLSGGKEAEAQLLSRAMKESVRRLSDETPLSLLGQTLDHYQVVSLSGKGGMGEVYRAR
                     DINLGREVAIKLLPESFPSGADRLRFEREARAVSALNHPNILTIHDIEQVDKYRFIVS
                     EFIDGDTLRKRLTNGPMRVGEALDIAIQVASALAAAHNAGIMHRDIKPENIMLRRDEL
                     VKVLDFGLAKVTESTLTSATPEQSTVEWFETDAGMVLGTSAYMSPEQTRGQAVDERTD
                     IWSLGVVLYEMVTGTQPFAGTTKSDVIVSILQKEPPALKDRTFASEPGLKRILQKALQ
                     KNVSERYQSARDLQDDLKALRQDLDTQKMIKPVASFPVRSRTVTYLLVVGIIAAAGFA
                     LYTIIGRNLSPKPQPELRTTPFTSFTGREQQPSFSPDGKQIAFSWNGDKGDNFDIYIK
                     LVNSETRPLRLTTDPAEDIYPAWSPDGKEIAFVRHQGPQITIFTVPVLGGQERKLYSG
                     TSAFYSLYEFGNALSWSPDGKYLAFSGQPGPRKPNGIFLLSLDTLGTRQITTPPEGFL
                     GDSTPAFSPDGKLLAFVRGASSRDVEVYVMPAVGGEPTRLTFDNRSGRSLGWTGDSKS
                     IVFSSWFYGGLLLWRINAGGGKPEQLNVGGEYASTLAVSRNGDELAYSQEFRDTNIWR
                     IELNGATAKSSATRLISSTRQDYSPQYSPDNKRIAFTSGRTGNNEIWMCDADGQNPVP
                     LTSFGGPDVGSPRWSPDSQKIAVDSLAPGHRDIYVMAVDGGKPRRLTGDNFDNVRPSW
                     SNDGNWIIFGSNRTGDWQLWKAPADGGEPVRITKQGGREGVESSDGKFIYYTKGFGVP
                     GIWKVPVAGGDETQVLDG"
     gene            2979..3371
                     /locus_tag="VFH91_02240"
     CDS             2979..3371
                     /locus_tag="VFH91_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861828.1"
                     /translation="MGLNERRKIKELQETTFPERVKEIEEICGKAVPYEVDWDSLSDD
                     LEALNFIDNISCHRLNMALRVICQDDLGKEAVREGLKLIKLKNVKDKSAMSLTFADGV
                     LEMHCAYALRTDGMFSDNEIRELLTQKL"
     gene            3581..>3879
                     /locus_tag="VFH91_02245"
     CDS             3581..>3879
                     /locus_tag="VFH91_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861829.1"
                     /translation="MKISHFVKNTIGTGFVLSLLLGIGIVSTVNTQAQYRYDPRYQQD
                     RDYRNQDRNDQNRDYRDRNYQRRGRSWDGYRNYGGSQQLRQTALNAGYNEGNKKGR"
BASE COUNT          945 a         1004 c          954 g          976 t
ORIGIN      
        1 ctccatcgag aacctgcgtt tcatcaccgc cagccaccgg cactttccag ataccaggta
       61 cgccaaagcc cttcgtgtaa taaataaact tcccgtcgct tgattcgacg ccttcccgtc
      121 caccttgttt tgtaatccgg accggttcgc ctccatcagc aggcgctttc cacaactgcc
      181 aatcgcccgt acgattcgaa ccaaaaatga tccaatttcc atcattcgac cagctcggtc
      241 taacattgtc gaaattgtct cccgttaaac gacgaggctt acctccgtcg acagccatta
      301 cgtaaatatc tcgatgcccc ggagccagag aatcaacagc aattttttgg ctgtcgggtg
      361 accaccgtgg agatccgaca tctggtccac caaatgaagt taacggcacc ggattctgac
      421 catcagcatc acacatccag atctcgttat ttccggtgcg ccccgaagta aaagcaattc
      481 gtttgttgtc tggtgaatac tgcggggaat aatcctgtcg agttgatgaa ataagtctcg
      541 ttgcactcga tttagctgtc gctccgttga gttcgatccg ccagatgttt gtatcgcgaa
      601 actcctgaga gtaagcaagt tcgtcgccgt tgcgtgatac agccagggtc gatgcgtatt
      661 ctccaccaac attcaactgc tccggcttcc ctcctccggc attgattctc cataacagca
      721 atccaccata gaaccaggat gaaaatacga tcgacttgct gtcgccggtc catccgagac
      781 ttctaccgct gcgattatca aaagtaagac gagtcggttc tcccccgacg gctggcatca
      841 catacacttc aacatcgcga ctagaagctc ctcgtacaaa cgccagcaat tttccatcag
      901 gcgaaaatgc cggcgtcgag tcaccgagaa atccttcagg aggtgtggtg atttgtcgtg
      961 ttcccagcgt gtcgagtgag agcaggaaaa tgccgtttgg ttttcgtggt ccaggttgac
     1021 ccgagaacgc tagatacttc ccatcaggtg accacgaaag tgcattgccg aattcataca
     1081 acgaatagaa cgccgaggtg cccgaataaa gtttgcgctc ctgccctccg agtaccggga
     1141 cggtaaagat ggttatttgt gggccctggt ggcgaacaaa tgcgatctcc ttaccgtcgg
     1201 gcgaccatgc gggatagata tcttccgccg ggtctgtagt gagtcgcaga ggtctggttt
     1261 ctgagttcac cagtttgatg tagatgtcga agttatcgcc tttatcccca ttccacgaga
     1321 acgcgatctg tttgccatcg ggtgagaaag agggctgttg ttcacgtccc gtaaaacttg
     1381 taaaaggagt cgtgcgcagt tccggctggg gcttcggcga gagattgcga ccaatgatcg
     1441 tgtacagcgc aaaaccggcc gcggcaatga ttcccactac cagcagatag gttaccgttc
     1501 gtgatcgcac ggggaatgat gcgacaggct taatcatctt ttgcgtgtcg aggtcttgtc
     1561 gtaaggcctt gagatcatcc tggagatctc tagctgattg ataacgttca gacacgttct
     1621 tctgaagagc tttttgaagg atccgcttta ggccaggctc acttgcgaat gtccgatctt
     1681 tgagggcggg aggctctttt tgcaggatgg agacaatcac atcacttttc gtcgtgcccg
     1741 caaaaggttg cgttccagtg accatctcat acaacacgac gcccaaactc cagatatcag
     1801 ttcgttcatc gaccgcctgt ccgcgagtct gttccggcga catgtaagcg gaagtgccga
     1861 gcaccatccc tgcgtcagtt tcaaaccatt caacagtaga ttgctcaggc gtcgcagaag
     1921 taagcgtcga ctccgtgact tttgctaatc cgaaatcgag gaccttaacc agctcgtctc
     1981 gcctgagcat gatgttttca ggcttgatgt cgcgatgcat aatgccagcg ttatgagccg
     2041 ccgcgagtgc actggctacc tgaatcgcga tgtccaacgc ttcaccgact ctcattggac
     2101 cgttggttaa tctcttgcgc aacgtatcgc catcgatgaa ctcagatacg atgaaacgat
     2161 atttgtcgac ctgttctatg tcgtgaatgg tcaggatgtt gggatgattc agtgccgaaa
     2221 ccgcgcgtgc ttctctttcg aagcgcagcc ggtcagcgcc cgaaggaaaa ctctcaggca
     2281 aaagcttgat tgcaacttca cggcccaggt taatgtcgcg ggcccggtag acttccccca
     2341 tgccgccctt tccactcagc gacacaacct ggtagtggtc caatgtttgc cccaacagcg
     2401 acagcggagt ctcgtcagac aatcgtctga ccgactcctt catagccctg gaaaggagct
     2461 gtgcctcggc ctccttccct cctgacaaca ggtatcgaac ttcactggtt aattcctcat
     2521 cgggaccgca gcgctgctcg agaaaggatt cgcggtccgg ctccgggcgc ttgagtgcct
     2581 catgataaat ctgtgagatt aattgaaatc tctcgggcgt catgatcgag ctatttggcg
     2641 cacacgacta gttgcgtggg ctcaaataat tacggttttc cttagaactt actacctgtc
     2701 cgtgtttatt taaccctgaa gtttagccaa ctattttctt tgccgcagtt ggccgctcca
     2761 gtggggctgg cttaataata gagggaagtt aacacaactg tccgtctgtt ttcacgtgga
     2821 aaaggcgtgc agtggtgtcc taatctgaac cttgtctgtg cttcctctga gatctcttcg
     2881 gtctctgtgg tgatggtttt cttctagcct tgcatctttc ctggacgtta tgcagactgt
     2941 tgccccacca tatctaccat cttctaaagg agttaatcat gggccttaac gagcgcagaa
     3001 agataaagga attacaggaa acaactttcc ctgaacgagt aaaagagatt gaagagattt
     3061 gcggtaaagc agttccttat gaagttgatt gggatagcct ttctgacgat ctcgaagcct
     3121 taaacttcat cgacaacatc tcatgccatc gtctgaacat ggccctgcga gtaatttgtc
     3181 aggatgacct cggaaaggaa gcagtacgcg aaggcctcaa actaatcaaa ttgaagaatg
     3241 tgaaagataa atctgcgatg agtttgacat tcgcagatgg ggttctcgaa atgcactgtg
     3301 cttacgctct ccgcaccgat ggcatgttca gcgacaatga gatccgcgag ctcctgacgc
     3361 agaagctata ggtgttgtaa ctaaacgcgt atcgaaggga tggaaattgc acgaactcgt
     3421 atacggacag tcattgttga gttagaccat ctgacgcccc aattgggcca taggcctatt
     3481 gttttactgg ataaatctca ggtttttatt atgacggggc tcttatcaag gtattgcggc
     3541 acaacccttg ctcctgtcag cgtagtagga gggtcggagc atgaaaatca gtcacttcgt
     3601 aaagaacaca attggaaccg ggttcgtact gtcactgttg ttgggtatcg gaatcgtttc
     3661 tacagttaat acacaagcac aatatcgata cgatccgagg taccaacaag atcgtgacta
     3721 tcgaaatcag gatcgtaacg atcaaaatcg cgactaccgg gatcgtaact atcagcggcg
     3781 aggtcgcagc tgggacggtt atcgaaacta cggtggctca caacagttga gacagacagc
     3841 gctcaacgcc ggctacaacg agggtaataa aaaaggccg
//
LOCUS       DASSFP010000106         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1959735, whole genome shotgun sequence.
ACCESSION   DASSFP010000106 DASSFP010000000
VERSION     DASSFP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959735"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFH91_02250"
     CDS             <1..475
                     /locus_tag="VFH91_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HET6861830.1"
                     /translation="EHVEAVWEGMADGTIDTIATDHAPHHSDEKALEFDQAPFGIVGL
                     ETAVGLAFELVHKGIIGLDRIVEMCSTNPARIFSLTDRGSLKPDAHADVTILDPQYNW
                     VFDSSKSKSKSRNTPFEGRAFQGASIATIVGGRLVYLHPDYTQITESRQRQASAK"
     gene            770..1498
                     /locus_tag="VFH91_02255"
     CDS             770..1498
                     /locus_tag="VFH91_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6861831.1"
                     /translation="MKSKVTGFTCMALVTLLLAPVCVADSNSSRVLKQQERYTLSLEL
                     EFTKKDQNPLEHAISVLFDVGPNGYATGFLVGNGLVMTAYHVISGNLSNTKKVMLGFR
                     PNDQLAVKVFVEGCRAKVLMVDKEADLALLEMCRNSKTAKAPVFQTAPSKNDKIFLIA
                     RPHGDKIVSHGSFYGNYMLGNQEYWSVKIDSRDGFSGSPVYNQRAEVVGVFSGYDWSQ
                     KLALISPSIRAQKLLEDYNANPRP"
     gene            complement(1555..2724)
                     /locus_tag="VFH91_02260"
     CDS             complement(1555..2724)
                     /locus_tag="VFH91_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6861832.1"
                     /translation="MAEVKGYDVAVVGAGVFGSWTAFHLQRRGARVILLDGYGPGNSR
                     ASSGGESRIMRLGYGRDRIYTDSAHRSLKQWLELFSHLSNSVSDSYQYFRRAGMLWLA
                     RDNDDYCEGVIENLQRLQARCERLDREQLESRYPQINFGSVSWAVLENDSGVIMARRA
                     VRAVVNESIKRGVKLKQRAVKPLNHLTTVNYLSLDNGEFVFADKFVFACGPWLPKLFP
                     DLLPEIIHVTKQEVFFFGTPPGDSLFRSDSFPAWIDFNDLVYGIPNLDERGFKIAIDA
                     HGDTFDPDTGQRIVSQAGIDAIRRYVSERFPILANAPIIETRVCQYENTSSGDFLIDR
                     HPDLENVWLAGGGSGHGFKHGPAVGEYISNLLEGGYAEPRFSLNSKERLPHRQVF"
     gene            2828..3352
                     /locus_tag="VFH91_02265"
     CDS             2828..3352
                     /locus_tag="VFH91_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET6861833.1"
                     /translation="MRLKNPDSFILSGLSLALTLIVAGSAFSAQRSLAGTQWRLDSFG
                     KAGDEKPILDGTSITIQFGTDGRASGSSGCNSYSGTYNAEGEHLSFGQMISTMRACVE
                     PKANQQEQTYMAALRAITRFTITESRLTLSDGGEVVLNFSSQSSSNGQGPGRYDSRRL
                     AELIFRGDKREALW"
BASE COUNT          921 a          905 c          865 g          862 t
ORIGIN      
        1 ggagcacgtg gaagcagttt gggaaggaat ggctgatggc actatcgaca ctatcgcgac
       61 cgaccacgca ccacatcatt cagacgaaaa agcactcgaa tttgatcagg ctccatttgg
      121 catcgtgggt ttggaaacag cggtcggttt agccttcgaa cttgttcaca aagggattat
      181 cggattagat agaatcgtcg agatgtgctc aacaaatcct gcccgaatat tttcgctgac
      241 cgacagaggt tcgctgaaac ctgacgcgca tgccgacgta acgatactcg atcctcaata
      301 caactgggtt ttcgactcgt ctaagtcgaa gtcaaaaagt aggaacacgc ctttcgaggg
      361 cagggctttt caaggagctt cgatcgccac catagttggt ggccgcctcg tgtaccttca
      421 tcccgactac actcagatca ctgagtcgcg tcagcgtcag gcatccgcaa aatagggttt
      481 cgccagaacg aaagaccgtt gctacacctc aaagcgagtt attcgctacc cgctcttttc
      541 gttccgctga aggccaggtc gcactaaaaa gattgcgaaa ccagcgacac agacctagaa
      601 tgctccaagc ctttagttca ctttagtttt cacccaaagt acaggtctta aatccttctt
      661 tggatctgcc cctcgtccaa ctaaagttgt tttgttagag cttttccacg ttacctcggt
      721 cagacaagac cgacgatcaa aaaattattg aaatcgaacg gaggccccga tgaaaagtaa
      781 ggttaccggc tttacctgta tggccctcgt cacccttctg ctggcacctg tttgcgtggc
      841 tgacagcaac tcatccaggg tgctcaagca gcaagagcgc tatacattgt cgctggagtt
      901 ggagttcacc aaaaaggatc aaaaccccct cgagcatgcc atctcggtat tgtttgatgt
      961 ggggccaaat ggctatgcca caggattttt ggttggtaat ggtttggtaa tgacggcgta
     1021 tcacgtcatc tctgggaatt tgagcaacac caaaaaagtc atgctcggat tcaggccgaa
     1081 tgatcagctg gccgtcaaag tctttgtcga gggctgcagg gcaaaagtac tgatggtcga
     1141 caaggaggca gatctcgcgc ttctagaaat gtgccgcaat tctaaaaccg ccaaggcccc
     1201 cgtatttcag acagctccaa gtaagaacga caagatcttt ctcatcgccc gaccgcacgg
     1261 agacaaaatt gtcagccatg gcagcttcta cggcaattac atgcttggga atcaggagta
     1321 ctggtctgta aaaatcgata gccgcgatgg tttctccggt agccctgttt acaaccaacg
     1381 agccgaggta gtgggcgtgt ttagtggcta cgactggtca caaaaacttg ccttgataag
     1441 tccaagtatc agagcgcaaa agttgctcga ggattacaac gcgaacccgc gtccctgagg
     1501 gcacaacttt ttcggcttgg gaccggcgtg gattgaatgc cgcgccggtt cttgttagaa
     1561 cacttgtcga tgaggaaggc gctctttcga gttaagagaa aagcgcggtt ctgcgtaacc
     1621 accttcaagc agatttgaaa tgtattctcc caccgctggg ccatgcttaa atccgtgacc
     1681 tgagccacca ccagccaacc acacattctc taagtccgga tgacggtcta tcaaaaaatc
     1741 gccacttgag gtgttttcat actggcacac gcgagtctcg ataatcggtg cgtttgcaag
     1801 tattggaaaa cgctcggaca cataacggcg aatagcgtca atcccggcct gggaaactat
     1861 tctttgaccc gtatcagggt cgaatgtgtc gccatgtgcg tcgatggcga tcttgaatcc
     1921 tcgttcatcc agatttggaa ttccgtagac caggtcgttg aagtcgatcc atgcagggaa
     1981 ggagtcagac ctaaagaggg agtctcccgg tggtgttcca aagaaaaaga cttcctgctt
     2041 cgtaacatgg attatttccg gtaataagtc tggaaacaat tttggcaacc agggaccgca
     2101 ggcaaacacg aacttatcgg caaatacaaa ctctccgtta tccaaactca aatagttaac
     2161 cgttgtcaga tggttaagag gttttacagc tctctgtttg agcttaacac cgcgtttgat
     2221 tgactcattc acaaccgctc ggaccgcgcg ccgcgccatg attacgccgc tatcattttc
     2281 gagcacggcc caactcactg atccgaaatt gatttgagga taacggctct ccagctgttc
     2341 acgatctaat ctctcacagc gtgcctgcag acgttgaagg ttctcgataa ctccttcgca
     2401 atagtcatcg ttgtctctgg ccagccataa catccccgcg cgccggaagt attgataact
     2461 atcgctcacc gagtttgaga gatgggaaaa caattccaac cactgtttca acgaacgatg
     2521 cgcagagtct gtatagattc gatcgcggcc gtaaccaagc cgcataatgc gtgactctcc
     2581 cccggagctg gctcgactgt ttcccggacc atacccgtcg agcaagatga ctctcgctcc
     2641 gcgacgctgt agatgaaaag cggtccatga gccgaatacg cctgcaccta caactgcaac
     2701 atcgtagcct ttgacttctg ccatacaatc ggattgtaac tttaggttaa gatttcaagc
     2761 caaacttttc tttgttccaa tcatcgacaa tgattaggag gcagccgtaa gaataggaga
     2821 agtgaagatg cgacttaaaa accctgattc tttcattctt tctggcctca gcctggcttt
     2881 gacgctcatt gtcgctggca gtgcgtttag cgctcaaaga tcgctagctg gcacgcagtg
     2941 gcgacttgat tcgttcggaa aagctggaga cgaaaagcca attttggatg gcacctcaat
     3001 cacgattcaa tttggcaccg atggtcgcgc gagtggctcg tccgggtgca attcctacag
     3061 cggcacttac aacgcggaag gagaacatct ttcattcgga caaatgatca gtacgatgcg
     3121 tgcttgcgtc gagcctaagg ctaatcaaca agaacaaacg tacatggctg cgttgcgagc
     3181 cattacgcga tttacgataa ctgaaagtcg tcttacctta agtgacggcg gggaggtagt
     3241 actcaatttt agtagtcaat cttcgtcgaa cggtcaagga cccgggcgct acgactcccg
     3301 tcgacttgct gaacttatat ttcgcggcga taaacgagaa gcactatggt aaagcgtacg
     3361 cacttctaaa agactcatgg aggaaaagat gacccggtcc aataagatca ttcttggttt
     3421 tgtttttaca ctcctattcg tcactgcttg tgccaatcct gcggctgata agtcgaaggc
     3481 agttactggg gaggctgcga cggcctcgcc acagtcagtc gcccagggag agcaatattt
     3541 aatcagtcca cag
//
LOCUS       DASSFP010000107         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2105363, whole genome shotgun sequence.
ACCESSION   DASSFP010000107 DASSFP010000000
VERSION     DASSFP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105363"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(492..1415)
                     /locus_tag="VFH91_02270"
     CDS             complement(492..1415)
                     /locus_tag="VFH91_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861834.1"
                     /translation="MPSSLGYLTVAIVLALGLFFGLWWMLVSGGDEAPWLPAGLAASV
                     VLLVALSAREVVMRRAWTRYLLENGIQQRSKRHSASSGRSQKKGFSASYHSAALRAIQ
                     KQSEAADTSGRPELHFDAAQLCHDYLLNTEEGLRNGSYGTEKVIAVRAGQERVRNLRR
                     HHLLVWARNQSQALTREAQTRVRTSDKIETANKALDCIDQALTAYPTETELKESRIAI
                     EEFIVSVKVAHWVELAERSAFKGHYRRAIDRYRDALYYLNHEAVKPEVRAAGAAKIER
                     EIDSLTAKLRARQREHEMIKENPEPEGDYPA"
     gene            1669..2445
                     /locus_tag="VFH91_02275"
     CDS             1669..2445
                     /locus_tag="VFH91_02275"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET6861835.1"
                     /translation="MAEKRFAGKTVLITGATSGIGRSCTIAFAEAGARTVCVGRNEQA
                     LAELSHHLEATGSEVLTVSTDLSFEGGAKKVVDVALQRFDGIDVLVNAAGHISSGTIE
                     DTSLEAWDNMMAINVRAVFSLMQAALPTLIARKGNVVNISSVTGLRSFPGVLAYCVSK
                     AALDQLTRCSALELAPKGVRVNAVNPGVVVTEIHKRGGMSDEKYAAFLEHSKQTHPLG
                     RVGRPEEIASLVLYLASDEASWITGATYSIDGGRAQTCAR"
     gene            2543..>2757
                     /locus_tag="VFH91_02280"
     CDS             2543..>2757
                     /locus_tag="VFH91_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861836.1"
                     /translation="MSSPNFYNASEQAATVVIKRSSQSRVLTLVLGIIAILIASLLGL
                     LVLLVIGVETGPVPFILGLFSAIIPVPL"
BASE COUNT          672 a          742 c          688 g          655 t
ORIGIN      
        1 tgctgagcaa ggacaatgct cgacaaagtt gcagcaagaa tcgttgcagt ccagactaat
       61 ttcgttctca taacaaaaac ctcaacttgt ctacgtttca aatcagcaga cagaagaaac
      121 tccaactgcg ggtaaggatg cctgacgtcg aaccgtaaac ctagcgtgat ttggcagtaa
      181 ccgcaagtct agaagacgaa atttcgtctt gccagcctag ccttcgctga tatggctcga
      241 acaacccaac atcccggctc atagttcacc agacgaagat ggcacgactc tatcgccttc
      301 ccggtctgat tgatgcccgc gactccttct cttgtgactc tttaacgctc ccgggaatac
      361 aaagcccgta aagagcagat tacagtggtc acacaacaag tggtaaattc cgaatattcg
      421 catcgggagg ggtgtgcgtt tataaccgcg ccgtattctg gaactacgac atttcgggca
      481 cttcagtaaa ctcatgctgg atagtcaccc tcaggttctg ggttttcttt gatcatttca
      541 tgctcgcgtt gtcgggcgcg aagtttcgct gtcaatgaat caatctcgcg ttcgattttc
      601 gccgctccgg ctgcgcgcac ttccggtttg accgcctcat gattcaggta atagagcgca
      661 tcccggtatc gatcaatcgc ccgccgataa tgtcctttga atgcggaacg ctccgcgagt
      721 tcaacccaat gggctacctt aacggaaaca ataaactctt cgatggcgat ccgcgattcc
      781 tttagctccg tttctgtcgg atatgcggtg agcgcttgat ctatacagtc gagggccttg
      841 tttgcggtct cgatcttgtc agatgttctg actcgagttt gagcctcacg cgtgagcgcc
      901 tgagactgat ttcgagccca cactaataga tggtgacgcc gaagatttcg tacgcgctcc
      961 tggcccgcac gcactgcgat caccttttca gtaccataac ttccgttacg aagtccctct
     1021 tcagtattca gaagatagtc atggcacaat tgtgcagcgt caaagtgcaa ttcgggtcgt
     1081 ccagaagtat cagccgcttc agactgcttt tggatcgcgc gcaaggctgc tgaatggtat
     1141 gaagcagaaa accctttttt ctggcttcgt ccactcgagg ccgaatgacg tttcgacctt
     1201 tgctggatgc cattctccaa tagatagcgg gtccacgctc gccgcatcac aacttcacga
     1261 gctgataaag ccacgagcaa aaccacactc gcggccaatc ctgccggcaa ccatggtgct
     1321 tcatcgccac cactgaccag catccaccac aagccaaaaa acagcccaag cgcgagaaca
     1381 atcgcgacag tcagatatcc aaggctggaa ggcatgcgac tgcgtgacgt tcgcggcggc
     1441 ccgtcggcgg aagattccga aggagatcgg tgatctctcg caaaaaactg atctgcggaa
     1501 ttatttccca acgaatgccc cttggtcacc caaagccacg tatcatgtta ggccggggat
     1561 ggctagaaaa aactgccgag gtgataatag tcaacttttt gggtttgcac tgtcaatata
     1621 tgatcgctta agagaacgaa taatcaactt cagactggaa acaagacgat ggcagaaaaa
     1681 cgatttgctg gaaaaacagt actcatcaca ggcgcgacct ctggaattgg ccgatcctgt
     1741 actattgcat ttgccgaagc aggggctcgc acagtttgcg tgggccggaa cgagcaggcc
     1801 ctggcggaat tatcgcatca cctggaagca acaggctcag aagtgctcac agtcagcact
     1861 gacctctcat ttgaaggtgg cgccaaaaaa gtagtggacg tcgctcttca aaggttcgac
     1921 ggtatcgacg tattagtaaa tgcggcaggc cacatcagca gcggcaccat tgaagacacg
     1981 tctctcgaag cgtgggacaa catgatggcg ataaacgtac gcgctgtttt cagtttaatg
     2041 caggctgcgc ttcccactct tattgcccgc aaaggaaatg tcgtaaatat ctcgagcgta
     2101 actggcctgc gatcgtttcc tggcgtacta gcctattgcg tctcaaaggc ggcattggac
     2161 caactgactc gatgcagcgc gcttgaactc gctccgaaag gagtcagggt gaacgctgtt
     2221 aatcctggtg ttgtcgtcac cgaaatccac aaacgaggag gcatgagcga tgagaagtat
     2281 gccgcattcc tcgaacactc aaagcaaaca catccgttgg gaagagtcgg tcgacccgag
     2341 gagatcgcat cgttggtcct atatctcgcc tccgacgaag cttcgtggat cacgggcgca
     2401 acttattcaa ttgatggcgg tcgagcacag acgtgcgcac gatgagactc tttgtcgttt
     2461 agcgtctggt cgaaatattc cgactcaggc tacactgccg attcgcgcgt gaagtgttgt
     2521 cagttcgttt ggcggcaata agatgagctc ccctaacttt tataacgctt cggaacaagc
     2581 tgccacggtt gtgatcaagc gttcatctca atcgagagtg ctgacgctgg tccttggcat
     2641 tatcgccatc ttaatcgcct cactcctcgg gttgttggtc ctgcttgtga tcggcgtgga
     2701 gaccggaccc gtcccgttca tcctgggact cttcagcgcg atcattccgg tacccct
//
LOCUS       DASSFP010000108         3881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_470027, whole genome shotgun sequence.
ACCESSION   DASSFP010000108 DASSFP010000000
VERSION     DASSFP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470027"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFH91_02285"
     CDS             complement(<1..278)
                     /locus_tag="VFH91_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET6861837.1"
                     /translation="MKAEEFELPPPSRVNYLNATYGLKSWLLTKDHKRIALLYLGSIT
                     FFFFIGGLYAMMIRLELLTPQGDFLQSTTYNKVFTQHGIIMIFFFLIPS"
     gene            complement(297..1313)
                     /gene="coxB"
                     /locus_tag="VFH91_02290"
     CDS             complement(297..1313)
                     /gene="coxB"
                     /locus_tag="VFH91_02290"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET6861838.1"
                     /translation="MPDSASTLSWKVDAIYFYLSGITLFFTLLISGILIFFVIRYRRR
                     SPYEIPRPIAGSHKLETLWTVIPFAISMTIFGWAASVYFQQYHPPDNAVEVYVVGKQW
                     MWKIQHSTGQREINELHVPVGRKIKLIMTTEDVIHDFFVPAFRMKADVIPGKYTTQWF
                     EATKTGTFHLFCAEYCGMNHSGMIGSVIVMEPREFDNWLSGNTNSASPAAAGQQLYQT
                     LGCVSCHGANGEGGRGPTLAGLVGRKTPLVGGQQVIADEAYIRESILNPQAKLVEGFG
                     PIMPTFQGQVSEDQILQLLAFIKSLPGGSSQPQGAASPAASGTAAPRPAASPAQQNRP
                     AAGQ"
     gene            complement(1337..2269)
                     /locus_tag="VFH91_02295"
     CDS             complement(1337..2269)
                     /locus_tag="VFH91_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET6861839.1"
                     /translation="MMEVISTSFSRHVAVTKNFLAITICVLAAFVNVMAQAGPPGPSS
                     PLYGARPESGTTSTGLPTALKDVKIEQKLDQQLPMDLQFKDENGNPVKLGQFFHDKPV
                     VISLVYYDCPMLCTQVLNGMVTSFRVLPFQIGKEFDVVTISFDPRETPTLASAKKKIY
                     VDFLPAKMRSDASIGWHFLTGDRESIEKITDAVGFRYHYDESTRQFAHASGIMLATPQ
                     GKLSRYFYGIDYPARDLRLGLIESSQNKIGSPVDQLLLYCYHYDPATGRYGAVIMNVM
                     RIAGIITLIGIVSMFFLLRRRPQTRVELKTGGAA"
     gene            complement(2279..2833)
                     /locus_tag="VFH91_02300"
     CDS             complement(2279..2833)
                     /locus_tag="VFH91_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861840.1"
                     /translation="MKKDGHQTETPDVSHIRNVEVTHEASDISVSGVLTFVAALTVVT
                     VAIYIGMWLLFGYFQKQEAKEKPPGPMALQKGQRLPPEPRLQGAPGFGVTLENGQTVK
                     LELSKPAAEYDELNREWTEVLKGKVSDQPGKPVSMPISEAIKKIATEGLPARANSAPG
                     NIQDFAVSVPTAASSGRMSEKRLQ"
     gene            complement(2847..>3881)
                     /locus_tag="VFH91_02305"
     CDS             complement(2847..>3881)
                     /locus_tag="VFH91_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861841.1"
                     /translation="IAVGSLALLMLQHLTGGGWGFVIRRVLEAATRTLPLMAVLFIPI
                     ILGAHSLYPWTREEVLKEEPIVAFKTPYLNLPFFSTRAVLYFAVWIVLAYFLNKWSLL
                     QDRTAERRYTKNMRLLSGPGMVFLIISITFAAIDWYMSLEPEWSSTIYGFIFVAAWSL
                     SALAFVIAVMAVLSKEEPMKRIVAPLHFHDHGKLLLALVMLWAYFAFSQFLIIWSGNL
                     PEEIGWYLKRTHGAWGVIALSIVILHFAAPFLFLLSRRIKRDPNKLVIIAVVILVMRF
                     IDLLWMIVPAFTGEHFHLTWMDLVAPVAFGGIWLAFFMWQLSKRSLIPINDPQYESVL
                     EQAAAGAHGH"
BASE COUNT         1020 a         1040 c          935 g          886 t
ORIGIN      
        1 gacggaatca aaaagaaaaa gatcatgatg attccgtgct gcgtgaatac tttattgtat
       61 gtagttgact gcaggaaatc gccttgcgga gttaacaact caaggcggat catcattgcg
      121 tagagcccac cgataaagaa gaaaaaagtg atggagccca ggtataggag cgcaatgcgc
      181 ttgtggtctt ttgtcagcaa ccacgacttc aggccatacg tagcattaag gtaattgacc
      241 cgcgatggtg gtggtagctc gaattcttct gctttcataa cttacgacct taaaacttat
      301 tgacctgcgg ccggcctgtt ttgttgcgct ggcgaagcag ctggtctcgg cgctgctgtt
      361 ccggatgcgg ccgggctggc agcgccttga ggctgacttg agcctcctgg caacgactta
      421 ataaaagcta acagttgcaa aatctgatcc tcgctaacct gaccttgaaa tgtcggcatg
      481 attggaccaa agccttcaac cagcttcgct tggggattca gaatcgactc tcgaatgtaa
      541 gcttcatcag caataacttg ctgaccgcca accagtggag tcttgcggcc cactagtccc
      601 gccaaagtcg ggccacgacc accctcgcca ttcgcaccat gacatgaaac gcaaccaagc
      661 gtttgataga gctgctgacc agccgcggcc ggactggccg aattagtatt tccgctgagc
      721 caattatcga actcacgcgg ctccatcacg atcaccgaac cgatcatgcc tgagtgattc
      781 atgccgcagt actcggcgca aaacaggtgg aacgttccgg tcttggtggc ctcaaaccac
      841 tgggtcgtat atttacccgg tataacgtca gctttcatcc ggaaagccgg cacaaagaaa
      901 tcatgaatca cgtcttcggt cgtcatgatc aacttgatct tgcgaccaac tggaacgtgc
      961 agttcattta tctctcgctg accagttgaa tgctggatct tccacatcca ttgtttgcca
     1021 acgacgtaga cttcgacggc gttatcgggc ggatggtatt gttggaagta cacactcgca
     1081 gcccaaccaa atatcgtcat cgatatcgca aagggaatca cggtccacaa tgtttcaagc
     1141 ttatgcgagc cggctatcgg tcgaggtatt tcgtacggac tgcggcggcg atagcggata
     1201 acgaagaaga ttaaaattcc tgaaattaac agggtaaaga aaagcgttat tccagacagg
     1261 tagaaataga tagcgtcgac tttccacgac aacgtggaag cactgtcagg cactagtgga
     1321 atccaggact gcattactag gccgcgcctc cagtcttcag ttcgactcgc gtttgcgggc
     1381 gacgacgaag caggaaaaac atgctgacaa ttccaatcaa ggtgatgatg ccggcgatcc
     1441 gcataacgtt catgatcacc gccccatatc gtcccgtggc cgggtcgtag tgatagcaat
     1501 aaagcagcag ttgatcgaca ggcgacccaa tcttgttctg tgatgattca attagtccca
     1561 ggcgtaaatc ccgagccgga tagtcgattc cgtagaagta gcgagaaagc ttgccctgcg
     1621 gcgtcgctaa catgattcca ctcgcatgag cgaactgacg agttgactca tcgtagtggt
     1681 agcgaaatcc aacagcatca gtaatctttt caatactctc acgatcaccc gttagaaaat
     1741 gccaaccaat gctcgcatcc gagcgcatct tggcgggaag aaagtcgaca tagattttct
     1801 ttttggcgct ggcgagcgtc ggagtttctc ggggatcgaa actgattgta acgacgtcaa
     1861 attcctttcc aatttgaaac ggcaacactc gaaaagatgt gaccatacca ttcagcacct
     1921 gagtgcagag catcggacag tcgtagtaga cgagcgaaat cacgactggc ttgtcatgaa
     1981 agaattggcc caacttaact ggatttccgt tttcgtcttt gaactgcagg tccatcggta
     2041 actgctgatc aagcttctgc tctatcttga cgtcctttaa agcagttggt aaccctgtcg
     2101 aggttgttcc gctttcagga cgagcaccgt agagcggcga gcttgggcct ggagggccag
     2161 cctgggccat cacatttaca aaagctgcaa gcacacaaat cgtgatcgcc aggaaattct
     2221 tcgtgacggc tacgtgcctg ctaaaactgg tactaattac ttccatcatc tttaacgcct
     2281 actgcaaacg cttttcactc atacggccag aactcgcggc ggttggaaca ctgactgcga
     2341 aatcctgaat gttccccgga gcgctgttgg ccctcgccgg caacccttcg gtcgcaattt
     2401 tcttgatcgc ttcgctaatc ggcatcgaaa cgggtttgcc aggttgatcg gacactttac
     2461 ccttaagcac ttctgtccac tctctattga gctcgtcgta ctcagctgct ggtttgctta
     2521 gttccagttt gactgtttga ccattctcaa gtgttacgcc aaaacccgga gcaccctgca
     2581 gacgaggctc aggcggcaat cgctgaccct tctgcaaagc cattggtcca gggggctttt
     2641 ctttagcctc ctgcttttga aagtaaccaa acagaagcca cattccgatg tagatggcga
     2701 ccgtaacaac agtcagtgcc gccacaaacg tcaaaacgcc cgacacgctg atatcgctcg
     2761 cctcgtgagt cacctcgacg ttgcgaatat gcgaaacgtc tggagtctca gtttggtgtc
     2821 cgtctttctt cataactaaa aatgaatcag tgtccgtgcg cgcccgctgc tgcctgttcc
     2881 agtacgctct cgtactgcgg gtcgttaatc ggtatcagtg aacgcttgct caactgccac
     2941 ataaaaaagg ccaaccagat tccgccgaac gcaacaggag ccacgaggtc catccaagta
     3001 aggtggaagt gctcaccagt aaaagcggga acgatcatcc acagcagatc aataaatcgc
     3061 attaccaata tcacgaccgc aatgattacc aatttattcg gatcgcgctt aatgcgcctc
     3121 gatagcaaga acaggaaagg cgccgcgaaa tggaggatga ctatcgacaa cgcgatcacc
     3181 ccccaggcgc catgagtccg cttcagatac cagccaatct cttctggtaa attgcccgac
     3241 caaatgatca gaaactgcga gaaagcaaag taggcccaga gcatcaccag cgccagcaac
     3301 aacttcccat gatcgtgaaa gtgaagtggc gccacaattc gcttcattgg ctcttccttc
     3361 gaaaggaccg ccatcactgc tatcacaaaa gcgagcgcgc tcaaactcca ggctgccaca
     3421 aagatgaatc cgtagatcgt tgaggaccat tcaggctcaa gagacatgta ccaatcgatc
     3481 gctgcaaatg tgatcgagat gatcaggaag accattcctg gtccactcag caaacgcatg
     3541 ttctttgtat agcgcctctc ggcagttctg tcctgcaaca gcgaccactt attcaggaag
     3601 taagcgagta cgatccagac tgcaaaatag agaacggcgc gggtcgagaa gaagggcaga
     3661 ttcaggtacg gtgtcttgaa agcaactatg ggctcttctt tcagcacctc ttctctcgtc
     3721 cacgggtaaa gtgaatgagc acccaaaata atcgggataa acagaaccgc catcaaaggc
     3781 agcgttctcg tagccgcctc aagcactcgc ctgatgacga atccccaacc accgccggtc
     3841 aaatgttgca acatcaacag cgcaagcgag ccaactgcta t
//
LOCUS       DASSFP010000109         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_172855, whole genome shotgun sequence.
ACCESSION   DASSFP010000109 DASSFP010000000
VERSION     DASSFP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172855"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="VFH91_02310"
     CDS             complement(<1..1737)
                     /locus_tag="VFH91_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861842.1"
                     /translation="MKGSVLTAKTWVLLIGAALLISAGLLNFRQRVRHETPAWDGVEW
                     VDTDHGVVAQTIERDSSGEKAWLLPGDHLLGISLDGVGKYDTIARARDVGMYLDQARV
                     GGQIHYFMERPSYPEDTRRYYADLDNLDAVHRWTPRVLYINIIGLVYLCVGFFVLFKQ
                     GGRAPYVLQFATLSLVAFVFHFYTPVGSYRDLDMAIAFLKNVALLMFPPMILHFCLLY
                     PSKHQLFATRRWRAVGLYIPALLLFLVCIVLFLRDELSKVLPPLQRIPVTETELVLFY
                     KIGIAQLILALVASALIVLRAFLNARNAVVRQQLKWVVWGTPLAVTPFTLLYGLGYLF
                     GARTDRWVTDAAVLPLVLIPLSLGYSILRYRLMDVELVVRRVFVYALTTLAIALCIGI
                     VVYTGGLYAFGSDQSFSSGEITLRVVVAVLAMAAIVMVAAPIKNFLQEQVDRVFYGER
                     YDLRNSLLDFGRTLSATTALDPLLDALVSRLQEVMNVERIAILIEDKRAPSGYAVARS
                     TGLSSEIAIPSDFREMIRVRSAETGIVRADDLDLNAEISGTVRRSLHYFVPCVVRGKM
                     VAVIGLGRSVDGA"
     gene            complement(2062..3822)
                     /locus_tag="VFH91_02315"
     CDS             complement(2062..3822)
                     /locus_tag="VFH91_02315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6861843.1"
                     /translation="MRRRLFLVLYSFLLASLVVNSSQAATPARPALATITGSVRDNKG
                     NPLAGALVSLLKEGVKNVVKEARTDRQGNFIAKVWPGRYGIRAIANGFTEVSFDAVDI
                     KASQELSYRFNLEPIGNGNTLPERRRDREDVKWVLRSAQSRRSVFQVREGEDADIQAV
                     TQNNSDDQNSVMETSADGSQPERLNEKSKPIGVVEAYSASDAYGSSYSGLNFAVSTPV
                     TDGVELIFAGQTGIGAAAPERLEASTRFNVGNRHKVGVTAAGLRLNSHPIFKTPSHQQ
                     QFLGQVSLRAIDEWIVRDGIVIVMGLDYSRFVGAGEAHSVTPRIGIQFDANARTRVKA
                     AFASGGDEDGIQSVASFEGEQVVFRKQTARPVAFIDGQAVLERSHRLEFGIERVLDNH
                     SNVEATAFFDTTTGRGVGLLSTPSTAFSGKAGESFINVANQEGSAKGLRVVYTRRLNR
                     VWTASAGYGFGQGQRLAAHGFTTPSEMFENAFFQTAALQLGAGFHTGTQIRTILRFSP
                     EATVFAIDPFAGRLGVYDPSLSIQVTQELPSFGLPVRAEAVLDARNLLDTQVSSENSE
                     STIVVGNNRRSVRGGISVRF"
BASE COUNT         1043 a         1051 c         1000 g          861 t
ORIGIN      
        1 cgcgccatca actgatcgtc ccagacctat cacggcgacc atcttgccgc gcacgacaca
       61 gggaacaaag taatgaagcg agcgacggac ggtaccagag atctctgcgt tgagatcgag
      121 atcatcggct cgaacaatcc cggtctcggc cgagcgaacg cgaatcatct cgcgaaaatc
      181 ggaagggata gcaatttccg atgacaatcc ggtggagcga gcaactgcat aacccgaagg
      241 tgctcgttta tcttcaatca ggatcgcgat ccgctcgaca ttcataacct cttgcagccg
      301 gctaaccagg gcatcaagta aaggatcgag agctgttgtt gccgaaagtg tgcgaccgaa
      361 atcaagcaga ctgtttctca aatcgtagcg ttcaccatag aaaacgcggt ccacctgttc
      421 ctgcaggaag ttttttatcg gtgccgcaac catcactatg gcggccatcg ccaggaccgc
      481 aacaacaact cgcagggtta tctcgccgga actgaagctt tgatcggatc cgaaagcata
      541 aagcccacct gtataaacca cgatgccgat acacagtgcg attgccagag tggtcaaggc
      601 gtagacgaac actcggcgga cgacgagctc aacgtccatc aagcgatatc tgagtatcga
      661 ataacccaac gaaagtggaa ttaaaaccag cggcaaaaca gctgcatcag tgacccaacg
      721 atcggtacgt gctccgaata aatagccaag accgtaaagg agcgtaaagg gtgttacagc
      781 caggggtgta ccccagacga cccacttcaa ctgctggcgt acaactgcat ttcgcgcgtt
      841 gagaaatgct cgaagaacga tgagagcact cgcaacgaga gcaagtatga gctgtgcaat
      901 cccaatcttg tagaagagaa ctagttcggt ctcggtgaca gggattctct gaagcggtgg
      961 taagactttg cttaactcgt cccgcaaaaa gagaacgatg caaaccagga acaggagcag
     1021 tgcaggtatg taaagaccta ccgcgcgcca tcgcctcgtg gcgaacaact gatgtttaga
     1081 cgggtacagt aaacagaagt gcaggatcat gggcggaaac ataagcaagg ccacgttctt
     1141 caagaacgcg atagccatgt ccagatcccg atagcttccg accggtgtgt agaagtggaa
     1201 tacgaatgca actaacgaga gtgtggcgaa ctgaagcaca tacggcgcgc gaccgccctg
     1261 cttaaaaagc acaaagaaac cgacgcaaag atagaccagc ccgatgatgt ttatgtaaag
     1321 gacgcgtggc gtccaacggt gaacggcatc gaggttgtca aggtcagcgt aatagcggcg
     1381 agtgtcttcg ggataggatg gccgctccat gaaataatgg atttggccac cgacacgggc
     1441 ctggtccaaa tacatgccca catcgcgcgc tcgggcaatc gtgtcgtact tgcctacacc
     1501 atcaaggcta attcccagca gatggtcgcc gggaaggagc cacgcttttt caccagacga
     1561 gtcacgctct atggtttgag caacaacacc atgatccgtg tcgacccatt cgacgccatc
     1621 ccaggccggc gtttcgtgac gtaccctttg ccgaaaattg agtagaccag cgctgataag
     1681 cagggctgcg ccgattagca atacccaggt tttcgctgtt agtactgagc ccttcactgg
     1741 tccttttact ccgcgcaaat tatcgagagg acagatccca ataaaacaag caagggttgt
     1801 tccggttgat gttccggttg atcaatcgct tcggattcat gtggcccctg ggctttcagt
     1861 gtggggagac acgcaaaatc gataaaggtg attcttttcc gggcgaagaa aagaaggagc
     1921 taagcgggat gaccatccaa cttttcggaa tcgtccgaga atttggacag tgcgcacttc
     1981 aggcgcgtcg gtaaaggaaa agcagaacaa cttctctttg gaaggggcta actagtcgac
     2041 tgctcgcttc gtggcgacgc attagaacct gactgaaata ccaccccgca cggaccgtct
     2101 attattgcca accacgattg tcgactctga attctcagaa ctcacctggg tatccaaaag
     2161 gtttcgcgca tcgagcactg cttcggcccg aaccggtaac ccaaaactcg gtaactcctg
     2221 cgtgacctga atactcagcg agggatcgta gacccccaat cgtccggcaa aaggatcgat
     2281 ggcaaaaact gtcgcttctg gcgaaaacct taagatcgtt cgtatctgag ttccagtatg
     2341 gaacccggcg cctaactgaa gcgccgccgt ctgaaagaat gcgttctcaa acatctctga
     2401 aggagttgta aacccatgag ccgcgagtcg ctggccttgt ccaaaaccat aaccggcaga
     2461 cgcggtccaa actctgttca agcgtcttgt atacacgacg cgcagacctt tagctgaacc
     2521 ctcctggttc gcgacgttga taaacgactc tccagccttc cccgaaaaag cagttgacgg
     2581 tgtgcttagt aagccgactc cgcgaccggt tgtagtatcg aagaaagctg ttgcttcaac
     2641 attcgaatgg ttgtcgagaa ctcgttcgat tccgaattcg agtcgatgac tacgttccag
     2701 cactgcttga ccgtcgatga aagctactgg tctcgctgtt tgcttacgga aaacaacctg
     2761 ctctccttca aacgaggcaa cgctttgaat tccgtcttcg tcaccgcccg atgcgaatgc
     2821 tgctttgact cgggtacgag cgttggcgtc gaattgaatt ccaatgcgag gcgtgacgga
     2881 gtgcgcttca cccgcgccaa caaatctcga gtaatccaac cccatcacaa tgacaatgcc
     2941 gtcacgaaca atccattcat cgattgctcg caaagacacc tgaccgagaa actgttgttg
     3001 atgtgacggt gttttgaaaa tggggtgtga gtttaagcgc aggcccgcag cagtaacacc
     3061 taccttgtgg cgattaccaa cgttaaatct ggtcgacgct tcgagtcgtt caggtgcagc
     3121 ggctccgatt ccggtctgtc cagcaaagat caactcaacg ccgtctgtaa caggtgtaga
     3181 gaccgcaaag ttgagtccgg aataagacga gccatatgcg tcactggctg aataagcctc
     3241 gacaactcct ataggcttag acttttcgtt gagtctctcg ggttgcgagc cgtcggcaga
     3301 tgtctccatc actgaattct ggtcatcaga attattttgt gtaaccgcct gaatgtctgc
     3361 atcttcacct tcgcgaacct ggaaaactga tcggcgactc tgtgcagatc tcaataccca
     3421 cttcacatcc tccctgtcac gacggcgttc gggaagggtg tttccatttc cgataggttc
     3481 tagattgaat ctgtaactga gttcctgcga ggccttaata tcgaccgcat caaatgagac
     3541 ctcggtgaat ccattagcga tggcgcgaat gccataacgc cccggccaga ctttcgcgat
     3601 gaaatttccc tgtcgatcag ttcttgcttc tttaactacg ttcttcacgc cttctttcag
     3661 aagtgatacc aacgcaccag caagcggatt acctttgtta tccctgacgg atccggtaat
     3721 agtcgccaac gctggccttg caggcgtcgc tgcctgactg gaattaacaa ccagcgaggc
     3781 aagaaggaac gagtacagga caagaaatag tcttcttctc atgcggtaaa acctccaggc
     3841 gcaaaactac ctagcggatg cagttgaacg aggcaagagc cggtcaaaga ttgattggac
     3901 tggtacaaat gactcctaag actttaactt tccacggtca cggtgccgtt gccgg
//
LOCUS       DASSFP010000110         9289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_20448, whole genome shotgun sequence.
ACCESSION   DASSFP010000110 DASSFP010000000
VERSION     DASSFP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20448"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFH91_02320"
     CDS             complement(<1..275)
                     /locus_tag="VFH91_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET6861844.1"
                     /translation="MSNQLQSASISEFRLLHESGCFILPNPWDIGSAVYLESLGFKAL
                     ATTSGGFAFSKGLPDGASAVTLDLTLAHLREIVDAVSLPVNADFQNG"
     gene            complement(433..1221)
                     /locus_tag="VFH91_02325"
     CDS             complement(433..1221)
                     /locus_tag="VFH91_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861845.1"
                     /translation="MQIRSRLFSLVIMVSLALSLSVIGSAQTKKKRSRRTSKPAAPKP
                     VITNPPIAAPATKSTSTGDVKIISTADQAPESESSDASASKGKPAGTSTPGEQETMQQ
                     TITTLSNQVNRLSDKLSSMQEDDRYTMDMERLTRAEQRAEQLRSQLFDLQSKLADAQA
                     RLEQIEYALKPENIERTTQSYGTVHPEEAREARRKQLEGEKSRVTAQLNLYESGKNRL
                     ETAVASADSEVDLLRAKLTQRREQMDAGSSNEARPATDSRPRKP"
     gene            complement(1407..2261)
                     /gene="nadC"
                     /locus_tag="VFH91_02330"
     CDS             complement(1407..2261)
                     /gene="nadC"
                     /locus_tag="VFH91_02330"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET6861846.1"
                     /translation="MNWFDEGALYSQIALFLREDLGRGDITTQTVIARNTRARARFVA
                     GETMIVAGLEAAEEVFLSLDSQQQLEAFVADGEQVEGGKVIARTIGFADVLLSAEQVA
                     LNLIQRLSGIATLTNQYVKALEGTECQVIDSRATSPGLRLLEKYAVKLGGGANGRFGL
                     DDGVLITANHLAIAGGIAKSLKITKEKLGHLHKLAVQVANEADAREAITNGADVLVTT
                     NPDPSAVATLVAVARELAAEIRVEYSGNVTPEKARSFAEAGASLISIEALTNAAPASK
                     ISFQMQPF"
     gene            complement(2258..4936)
                     /locus_tag="VFH91_02335"
     CDS             complement(2258..4936)
                     /locus_tag="VFH91_02335"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET6861847.1"
                     /translation="MDIPKQYDPAQAEKDHYERWEKNGYFAPEINKDPAAPVFTIVIP
                     PPNVTGSLHMGHALQHTMMDVMTRHKRMSGYRTLWLPGTDHAGISTQLMVTNALKEEG
                     KTRFDLGREKFVQRVWKWKAESGGQITHQMRREGASVDWSREKFTMDEDLSRAVREVF
                     VRFYEEGLIYRGKRIVNWCPRDQTVLSDLEVDKVPQAGKLYYLQYPIKNTDRHLTVAT
                     TRPETMLGDTAVAVNPSDERYGDLVGATVLLPLTGREIPVIKDDYVDPAFGTGAVKIT
                     PAHDPNDYEMGLRHDLPQVVVIDAHAKMTADAGADFVGLDRYKAREKVVEEFEKLGLL
                     EKVVDYEFSISKCERCKTVIEPLISEQWFMHMEPLRDSALELLAQKQKPKFTPDVPYE
                     RIYTNWLENLRDWTISRQLWWGHQIPAWYTPSGEVVVARSEAEAREKAGTAELVQDQD
                     VLDTWFSSALWPFSTLGWPDETEDLRTFYPTSVLVTARDIIFLWVSRMVMMGMKFIGQ
                     EPFADVFVTGTILDSQGQRMSKTKRNGVDPLIVFDKFGVDATRLTLAQVGSTDIRWNE
                     KQVESYRNFANKIWNATRFCLLNSEDAEVNPELLKTKDLALHDRWIISRLNKTAQRVN
                     ESLNTYQFHESVQTLYHFFWDDFCDWYIELSKSEVTAEEPSQKRTQARSRLLTVVEQA
                     LRLLHPFMPYITEELWQRLPGEKPLHPAYSGASSTIMLTSYPISSQEMTDDQAEQEMS
                     AIIDLISRVRNIRSEMNIKPAERIPVLVGCPDQELRNTFSSARDQIARLVRAADIQVS
                     DHLTAPKASARAVLVGGAELAVPLEGLIDFEQERQRLSREQDKLRAEATKLEAQLANP
                     SFVERAPAERVTEVQARISDIAQRNSQLQQTIENLQ"
     gene            complement(5110..6228)
                     /locus_tag="VFH91_02340"
     CDS             complement(5110..6228)
                     /locus_tag="VFH91_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6861848.1"
                     /translation="MLAGRKLLLADDSVTIQKVIDLTFADEGVRVVSFGDGQQAIDNL
                     EEVAPDIVLADIFMPSKTGYEVCKYVKENEKLKHIPVMLLVGSFEPFDEAEARRVGAD
                     DILTKPFQSIRRLIDRVGALVNNPPAKREVPTAELPKVKEESDANQFLSTSELEIATA
                     DTLRLPDNYDFGAKTLEPAVPAESVSKEEKMGSSEKRKSDSAPEASEVLLDLGEFEPV
                     HAAATNDDFVLDLEDTIEVSEPAVRPFVEPQVTERAATAAAHEYSSYQPAVIDAPFVD
                     TKELVIETQAHEVEVDDAPARYEPSAQVDRQAVASAAYASGVNADSLSPEMIDAIARR
                     VVEMMSDKVVREIAWEVVPDLAELLIQRRLAEKESQTK"
     gene            complement(6514..7401)
                     /locus_tag="VFH91_02345"
     CDS             complement(6514..7401)
                     /locus_tag="VFH91_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HET6861849.1"
                     /translation="MRSRRTVSLLALLLIMLSVDTARADLSHSQARKLISNISGISFP
                     RSEIHVVNVTQDNGSSANVTAQIELAFRATQNKNRVWRLSEVRFGPERWEDLSVISRS
                     GNFQLSEPDCKTGTKSTHLKNSEARCLVAGLFGITLPSDDIRIKELSGFGLLFGPRAS
                     ALIVSLVELNFRFSKDKGDWRLTGFKSGNGDWSDVGTLPQSLNAEKQALAKADLALIV
                     KALDSYRRDRGSFVVSEKESVVIDHLCPGYLGRVIRLDPWHRPYQYHGERDRFTLRSL
                     GPDGKPDTADDIVATNSNP"
     gene            complement(7414..7833)
                     /locus_tag="VFH91_02350"
     CDS             complement(7414..7833)
                     /locus_tag="VFH91_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6861850.1"
                     /translation="MASDEESIDSSDGENRRILVADDDPAILRLVATILEKENFNVVT
                     ARDGREAYKTLQGDPDFTAAILDVVMPHITGPELVRFMKSEPKLANIPVMMMTAEQDP
                     KLSSDSFSAGAVVFLPKPFTTAQLQIMLQMLIGKASS"
     gene            complement(7861..8928)
                     /gene="pdxA"
                     /locus_tag="VFH91_02355"
     CDS             complement(7861..8928)
                     /gene="pdxA"
                     /locus_tag="VFH91_02355"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET6861851.1"
                     /translation="MRQSDHSSTNPGPRPRRDLPRIGITMGDPAGIGPEVVLKAVAEL
                     EVRKACVPVIIGDAQMLAHTARTLDLQCGYDIIRKEETFPEHFSDPVIFHLDNISGFI
                     EPGIESGAAGKAAAGYIEAAVELCAAGSIDAIATAPINKRALFLGGYSFPGHTEFLAH
                     LTRSEEYAMAFVAANLRIVLLSTHVPLSEAIRMVERDRLVKIINLTNRELNRWGLERP
                     RLAVAALNPHGAEGGLFGVEEASEIMPAIAACRELDDINVHGPYSADTVFLRASRGEF
                     DAVIACYHDQAMIPVKCLSFGEAVNVTLGLPFIRTSVDHGTAFDIAGKGLAEHSSMVA
                     AIKLAADLSTQAGESCRPVEI"
     gene            complement(8991..>9289)
                     /locus_tag="VFH91_02360"
     CDS             complement(8991..>9289)
                     /locus_tag="VFH91_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861852.1"
                     /translation="NDGGQQRPAQQHTPVQVKKRVPPPNETNAEIFYYKKQIDAHTVM
                     VIVLMDGEEIEGTIEWYDRGALKVNRKAAPNLLVLKRNIKYMYKADERVETTGN"
BASE COUNT         2183 a         2456 c         2280 g         2370 t
ORIGIN      
        1 aagccgttct gaaagtcggc attcacagga agcgaaactg catccactat ttcacgcagg
       61 tgggccaacg taagatcgag tgtcacggcc gaggcgccat cgggcaatcc tttactaaaa
      121 gcgaaaccgc ctgatgtggt cgcgagagcc ttaaacccaa gagattctaa atagactgcg
      181 ctgccaatat cccagggatt gggcaagata aaacagccgg actcgtgaag gaggcgaaac
      241 tcggatatgg aagcggactg cagttggtta gacatcgaca tttctcattt ctcatttctc
      301 atttttcatt tactggccct cgagatggtc gcttccgttt cgttctagcg cagtcctcat
      361 acataaagag agcgagtctg ccgtcacaga aaaatagaaa atgataaatg gaaaatgacc
      421 aatgacaaat cttcagggct tgcggggacg ggagtctgtc gccggacgag cttcattact
      481 cgagccagca tccatctgtt cgcgacgttg agttaatttt gctcgcaaga gatcaacttc
      541 ggagtcagcg ctggccactg ctgtttccaa tcggttcttt cctgattcat acaagttcaa
      601 ctgcgctgtg actcgagact tctccccctc aagttgtttg cggcgcgctt ctcgagcttc
      661 ttcaggatga accgtgccgt aggattgagt agtcctttca atattttccg gcttcagcgc
      721 atattctatc tgttcaagcc tcgcctgagc gtcagctagc ttcgactgaa ggtcaaaaag
      781 ctgcgatcgc aattgctctg ctctttgctc tgcgcgtgtt aaccgctcca tgtccatcgt
      841 gtaacggtcg tcttcctgca ttgaactcaa cttgtcggac aaacgattga cctgatttga
      901 gagagtggta atcgtctgct gcatcgtctc ctgctcgcca ggcgttgaag ttccagccgg
      961 tttgcctttc gaagcagacg cgtcggagct ttcgctctct ggagcctgat ccgcggtgct
     1021 gatgatcttt acatcgccag tgctggtaga cttcgtcgca ggagcggcta tgggcggatt
     1081 agtaatgacc ggtttgggag ccgcgggctt actggtgcgt ctagaccgtt tctttttcgt
     1141 ttgcgcgctg ccaataacac tcagactgag cgccaaactg accatgatca ccagactaaa
     1201 cagacgagat cgaatttgca tctcacgaaa ctccttttat tgcgacttaa agatacgctt
     1261 gaaacaaaga tgccttcaag ggtaggccga aatgctttga actgtcacgt tcccaaacag
     1321 accgcgttcc aaaaaggttc cagcagcgac tcgtaacgtt ctagctctcg cgaccgccag
     1381 gtcccggcta ttaattcgga aggcgctcaa aacggctgca tctgaaaact aatcttacta
     1441 gctggagcag cattcgttaa cgcctcaatg gaaattaagc tggcgcccgc ctcagcaaac
     1501 gatcttgcct tttccggcgt tacgttacct gaatattcta cccggatttc agcagcaagc
     1561 tcacgggcga ctgctaccaa cgtcgcaaca gcagacggat caggattggt agtaactaag
     1621 acatcggctc catttgttat cgcctcacgt gcgtccgcct cgttggcaac ttgtaccgcg
     1681 agcttatgta agtgccccaa cttctctttg gtgattttta gactctttgc tatgccgccg
     1741 gcaatcgcca aatggttcgc tgtaatcaag accccatcgt ccaatccgaa tcgtccgttt
     1801 gcgcctccgc caagttttac agcgtacttt tccagcaggc gtaaaccggg agaagtcgct
     1861 cgactatcga tcacctgaca ctcggttcct tccaaagcct ttacatattg gtttgtcagg
     1921 gtagcaattc ctgacaaccg ttgaatcagg ttgagagcta cctgctcagc agaaagtaac
     1981 acatcggcaa atccaattgt tctggcgata actttaccgc cctccacctg ctcaccatca
     2041 gccacgaacg cttccagctg ttgctgagag tcgagcgata aaaagacttc ctccgccgct
     2101 tcgagacctg ccacgatcat cgtctcgccg gcgacgaaac gagcccgagc tcgagtgttt
     2161 ctggcaatta cagtttgcgt tgttatgtcg ccgcgtccga gatcttcacg caagaacaag
     2221 gcgatctgtg agtacagagc tccctcatca aaccagttca ttgcaagttc tcgattgttt
     2281 gttgaagttg agagttcctc tgggcaatgt ccgagatacg tgcctgaacc tcagtcactc
     2341 gttccgcagg cgctcgctct acgaaactcg gattggcgag ttgcgcttcg agtttcgtag
     2401 cctcggctcg tagtttgtct tgttcgcgac tcaaccgttg acgttcctgt tcaaagtcaa
     2461 tcaatccttc cagggggacc gctaactcag ctcccccgac aagcactgcc ctggctgaag
     2521 ctttcggagc agttaagtga tcagacacct gaatgtcagc cgcgcgaact aaacgcgcaa
     2581 tctgatctcg agcgctcgaa aatgtatttc tcaactcttg gtcggggcag ccaaccagca
     2641 ccggaatgcg ttcagcgggt ttgatgttca tttcggaacg tatgtttcgg acacgcgaga
     2701 tgagatcaat aattgcactc atctcttgtt cagcctggtc gtcagtcatt tcctgagaac
     2761 taatcggata ggaagtaagc attattgtcg acgaggcacc ggagtaagcc gggtgtagag
     2821 gtttttcacc gggcagacgt tgccatagtt cttcggtgat gtaaggcatg aacggatgca
     2881 gcagtcgtaa tgcttgctct actacagtga gtaagcgcga acgagcctga gttcgttttt
     2941 gcgaaggttc ttccgccgtg acttctgatt tcgataattc gatataccaa tcgcagaagt
     3001 catcccagaa gaagtgataa agcgtctgca ctgactcgtg aaattgatat gtgttcagag
     3061 actcgttgac gcgctgtgct gttttattca gccgagagat tatccagcgg tcatgaaggg
     3121 cgagatcttt cgtcttaaga agttcagggt tcacttctgc gtcttcagaa ttcagcaggc
     3181 agaagcgagt ggcattccag atcttgtttg cgaagtttcg gtacgactcg acctgtttct
     3241 cattccagcg gatatcagtg ctacccacct gcgcaagagt caaacgcgtc gcatcgactc
     3301 caaacttgtc aaaaactatg aggggatcca ctccgttcct ctttgtcttc gacattcgtt
     3361 ggccctgact gtcgaggatg gttccggtga caaacacatc cgcgaacggt tcctggccga
     3421 tgaatttcat gcccatcatc accatgcgcg atacccagag aaaaatgatg tctctggcag
     3481 taacgagaac agaagttgga taaaaagttc gcaggtcctc agtctcgtca ggccaaccca
     3541 aagtggaaaa gggccataag gccgacgaaa accaggtatc taaaacatcc tgatcctgaa
     3601 ctagttcagc ggtgccggct ttctctcgtg cttctgcttc cgatctggct acgacaactt
     3661 ctccagacgg tgtgtaccag gctggaattt gatggcccca ccatagttgt ctggagatgg
     3721 tccaatcacg aaggttctcg agccaatttg tgtagatccg ttcgtaggga acatccgggg
     3781 taaacttcgg tttttgtttt tgggccagca gctcaagggc cgaatctcgc aacggttcca
     3841 tgtgcatgaa ccattgctca gagatcaaag gctcgataac tgtcttgcaa cgttcacact
     3901 tggaaatcga aaactcgtag tcgacgacct tctcgagaag cccgagtttc tcaaactctt
     3961 cgacgacttt ttcgcgcgcc ttgtacctat cgaggccaac aaaatcagcg cccgcgtctg
     4021 cagtcatctt tgcgtgagca tcaatcacga caacctgagg cagatcgtga cgtaagccca
     4081 tttcgtagtc gttcggatca tgtgcgggag taatctttac cgcgccagtg ccaaatgctg
     4141 gatcgacata gtcatccttg attaccggaa tctctcttcc agtcagcggc agtagcactg
     4201 tggcgccgac aagatctcca tatcgctcat ctgaaggatt gaccgcaacc gccgtatcac
     4261 caagcatggt ctcaggccgc gtggtcgcaa cggtcaagtg acgatcagtg ttcttgattg
     4321 gatattgaag gtaatagagt ttgccggctt gcgggacttt atcgacttcg agatcagata
     4381 agactgtctg atctcgtgga caccagttaa cgattctttt tcctcgataa atgaggcctt
     4441 cttcataaaa acgtacgaac acttcgcgga ccgcgcgcga cagatcctcg tccatcgtaa
     4501 atttttcgcg cgaccagtcg actgaagcgc cttcgcgtct catctggtgc gtgatctgtc
     4561 cgcccgactc agctttccac ttccaaacac gttgaacgaa cttctctctt cccaggtcga
     4621 agcgagtctt accttcctcc tttaacgcgt tggttaccat caactgagtg gaaatacccg
     4681 cgtggtcagt gcctggcaac cagagggttc gataaccact catccgcttg tgacgggtca
     4741 taacgtccat catcgtgtgc tggagcgcgt ggcccatgtg tagcgagcca gttacattcg
     4801 ggggcggaat gacgatagta aagacgggcg cggcgggatc cttattaatt tcaggagcaa
     4861 aataaccgtt tttttcccag cgttcgtagt ggtctttctc tgcctgggcc ggatcgtact
     4921 gtttagggat gtccatggat gatctgaact tttgactgag atttttactc gagcattgaa
     4981 agtgtaagcc tttaacgtac aacgtactcc gcccaactga caactggcgt caacttaggg
     5041 tgctgatccc aaattaaaac aggtgaccgg gaacccagtc acctgtaaaa actttcaacg
     5101 cactaagttt tactttgtct gagactcttt ctctgccagc ctgcgctgta tcagcaactc
     5161 ggccaggtcc ggcaccactt cccacgcgat ttcacgaacc actttatccg acatcatttc
     5221 gactacgcgt cgcgcaatcg cgtcaatcat ctctggcgac aagctatcgg cattgacacc
     5281 gcttgcatat gcagcactcg ccacagcttg tcgatcaact tgagccgacg gttcataacg
     5341 tgccggcgca tcgtcaacct caacttcgtg agcctgagtc tcgataacca gctcttttgt
     5401 atcaacaaaa ggagcgtcga ttacggctgg ctgatatgaa gaatactcat gggccgcagc
     5461 agttgctgct ctctcagtta cctgtggttc aacaaaggga cgaactgctg gctcagaaac
     5521 ctcgattgta tcctcaagat ccagcacgaa atcgtcgttg gtggcggcgg catgaacagg
     5581 ttcgaattca ccgagatcca gtaggacttc ggacgcctct ggggcagaat cgcttttcct
     5641 cttttcgcta cttcccatct tttcctcttt cgagacggat tctgcgggaa ccgctggttc
     5701 taatgtctta gctccaaagt cgtagttgtc gggcaacctg agagtatccg cagtcgctat
     5761 ttccagctcg ctcgtgctga gaaactggtt ggcgtcggat tcttccttga ctttagggag
     5821 ttccgcagtg ggcacttcac gctttgccgg agggttatta acgagtgctc ccactctgtc
     5881 gatcagtctt cgaatcgatt gaaatggttt ggtcaatata tcgtccgcgc caactctgcg
     5941 cgcctccgct tcgtcgaaag gctcaaacga gccaacaaga agcattacag gaatatgctt
     6001 gagcttctcg ttttctttga cgtacttaca gacttcatag ccggttttgg acggcatgaa
     6061 gatatctgcg aggactatgt cgggcgcaac ctcctcgagg ttatcgatgg cctgttgacc
     6121 atcgccgaaa gagaccaccc gcacgccttc atcggcgaac gttaggtcaa tcactttctg
     6181 aatggtgacc gagtcgtcgg caagtaggag tttgcgacca gctagcaaag ctaaattcct
     6241 ttagctagaa gtagcaccat cggcggggcc tggtagtgcg tttgcacaga tggcgcgggg
     6301 catgtcataa cgaggtggcc tttgaaatcg ggccaccaga tgaaaactag cacccacggt
     6361 aactgacagt caagcgttag ttttagcacg ttcaacaaac tttggaatgc tggcggcccg
     6421 gcgccgtctt cccagacctg cctgttctta cgagcgctcc ttagtaaatc tgtccttaaa
     6481 aacaaagcgg tgccgggaca ccgcactcca aggttaagga ttcgagttcg tagccacaat
     6541 atcgtcggcg gtgtccggtt ttccatcagg tcctaaggat ctcaacgtga agcgatcccg
     6601 ttcgccatgg tattgatatg gccgatgcca gggatcgagc cggattaccc ggcctaaata
     6661 acctggacag aggtgatcga ttactaccga ttctttttca gagacaacaa atgagccacg
     6721 gtcgcgccga tatgaatcca acgccttaac gattaaggct aaatcagctt tggcaagtgc
     6781 ttgcttctca gcatttagtg actgaggcaa tgttccgaca tcgctccagt cgccattacc
     6841 actcttaaag ccagtaagtc tccaatcacc tttatccttt gagaaccgaa aattgagttc
     6901 gacaagggaa acaatcaaag ccgacgctcg aggaccaaac agcaatccaa atccggacag
     6961 ctccttaata cgaatgtcgt ctgacggaag cgtgataccg aagagacctg cgactaagca
     7021 acgcgcctcg gaatttttaa gatgagtaga tttcgttcca gtcttgcaat ccggttctga
     7081 cagctgaaaa ttgccagatc tggaaatcac cgacaggtct tcccatcgct ctggcccgaa
     7141 ccggacttcg ctgagccgcc acactctatt cttgttctgc gttgcacgaa acgcgagttc
     7201 aatctgcgcc gtgacattcg ctgacgatcc gttgtcttga gtgacgttaa caacgtggat
     7261 ctcgcttctt ggaaacgaga taccggaaat gtttgagatt aacttgcggg cctggctgtg
     7321 gctcagatct gcccgtgctg tatcaacgct cagcatgatc agcaacaaag cgagaagcga
     7381 aacagttcgg cgagatctca ttcgaaagtg gggttaactt gatgctttac ctatcagcat
     7441 ctgtaacatt atttgcagct gagcagttgt gaaaggcttt ggaagaaaca cgactgcgcc
     7501 ggccgagaaa ctatctgaag acagtttggg atcttgttca gccgtcatca tcatcactgg
     7561 aatattcgcg agcttgggct ctgacttcat gaacctaacg agttcgggac cagtaatatg
     7621 aggcatcaca acatccagga tggcggccgt aaagtctgga tcgccctgaa gagtcttata
     7681 agcctcgcgt ccgtcgcgag ctgtcaccac attgaaattt tccttctcca gaattgtggc
     7741 aaccagccgc aagatggctg gatcatcatc ggctactaga atgcgtcgat tttcaccgtc
     7801 gcttgagtcg atcgactctt cgtcactagc cattttgacc tgctctccct tcaatcattt
     7861 ttagatttct actgggcggc aactctcgcc agcctgagtt gatagatcgg ccgccaattt
     7921 tatcgccgcc accatactgg aatgttcggc cagtcccttg ccggcaatat cgaacgccgt
     7981 tccatgatcg acggatgtcc tgatgaacgg caatccaagc gtaacattaa ctgcttcgcc
     8041 aaaggacaaa cattttactg gaatcatcgc ctgatcatga taacaggcaa tcacggcatc
     8101 gaattcacct ctggaagctc tgagaaagac cgtatcggcc gaatacggac catgaacatt
     8161 gatgtcgtct agttcgcgac acgccgcaat cgccggcatt atctcggaag cttcttcgac
     8221 gccgaataga ccgccttcgg cgccatgtgg gttgagagct gcaacggcta gtcgaggtcg
     8281 ctcgagaccc caacggttca actcccggtt agtgagattg attatcttga ccagtctgtc
     8341 tcgttcaacc atccggattg cttcagaaag cgggacgtga gtggacagga gtactatcct
     8401 tagattcgcg gccacgaaag ccatcgcgta ttcttcggac cgcgtgagat gagcgagaaa
     8461 ctcggtatgt ccgggaaaac tgtaaccgcc tagaaataga gctctcttat taataggagc
     8521 agtagcgatg gcgtctatgc ttccggcggc gcaaagttcc acagcggctt cgatataacc
     8581 tgccgcggct ttccctgccg cacctgattc gattccaggt tcaataaatc cactaatgtt
     8641 atcgagatga aagatgactg gatccgaaaa atgttcagga aacgtctctt cctttcgaat
     8701 gatgtcgtag ccacactgga gatcaagggt gcgggcggtg tgagccagca tttgagcgtc
     8761 gccaattatg actggaacac acgcttttcg gacctcgagt tctgcgaccg ccttaaggac
     8821 gacctcaggt ccgatgcccg cagggtctcc catcgtgatg ccgatgcgcg gcaaatctcg
     8881 cctgggtcga ggtcccgggt tagttgagct atggtctgac tgtcgcattt gttgtggcga
     8941 cgcactgtta gggcggatga gttacacagg gcgaggctgt cgcaacctaa ttagttacca
     9001 gtagtttcaa cccgctcatc agccttgtac atgtacttga tgttcctttt gagaacaaga
     9061 aggtttggcg cggctttgcg atttaccttt aaagcaccac ggtcatacca ctcgatagtt
     9121 ccctcgattt cctctccatc catcagaacg atcaccataa cggtgtgggc atcaatctgt
     9181 ttcttgtaat agaaaatctc cgcattggtc tcgtttggcg gcggcactct tttctttact
     9241 tgaactggtg tgtgttgttg agcgggtctc tgttgaccgc catcgtttg
//
LOCUS       DASSFP010000111         5167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_470240, whole genome shotgun sequence.
ACCESSION   DASSFP010000111 DASSFP010000000
VERSION     DASSFP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470240"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1333
                     /locus_tag="VFH91_02365"
     CDS             32..1333
                     /locus_tag="VFH91_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6861853.1"
                     /translation="MELQVGLTLVLLIWLSLLATVDMAFGQLSDVGLRRLIGENGDKP
                     ERRSRGFLKQILENRPRFGFALSATIQILLVVVAVLITSISLTLFPDPRLVLVGLAAG
                     LILTGIFRQLIPLFISTRNPEGTLLFLLPIVRPFFPVMILISDPFQRLFDRSRKREII
                     SDDTTTEEENDDSDDLQALIDVGEAEGILEEEEGELIHSIIEFGDKRVSEVMTPRPDI
                     VAVSTTSSVREARDVMIESKYSRLPVYREHIDNVEGLIYVRDLLQFWAEGREDQNIRP
                     LIRSVYFIPETKPVGELLEEMQKAHVQLTMVIDEYGGICGLVTVEDILEEIVGEIEDE
                     DIAGEELVDIVDAGQGSYDVLASTEIGKIERLFDMEIEADDFTTIAGLVINESGKVPR
                     VGERLTIRSLEVEVLEADDRRINRLRVKKAQHENASEVSAS"
     gene            1755..2885
                     /gene="speY"
                     /locus_tag="VFH91_02370"
     CDS             1755..2885
                     /gene="speY"
                     /locus_tag="VFH91_02370"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HET6861854.1"
                     /translation="MVKHKSKFLKGRRIDPAPITKQIEAADLIDESFLAYNAARLREA
                     CHLFTKKMLDPDVTVGLSLTGALTPAGLGISALIPLIKAGFVDWIISTGANLYHDTHF
                     GIGLAMHQGNAQTSDIVLREEEVVRIYDIFFDYSVLLDTDAFFRKIIEAPEFQRAMST
                     AEFHYLCGRYVHEREQKLGISEKSLLGVAYEYAVPIYTSSPGDSSIGMNVAAKALQGN
                     KLAFDPSIDVNETASIVLSAKRGIGSKKNGKKQAGKSAVFILGGGSPKNFMLQTEPQI
                     QEVLGIDERGHDYFLQVTDARPDTGGLSGATPGEAVSWGKVDPDRLPDAVVCYVDSTV
                     ALPLITAYALTRHEPREPKRLYDRREEFMKLLESEYKKSKRR"
     gene            2911..3324
                     /locus_tag="VFH91_02375"
     CDS             2911..3324
                     /locus_tag="VFH91_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET6861855.1"
                     /translation="MSTSPPTVKPKSYYDCVDCPAYCCSVYERVQVTPRDIRRLAKHF
                     AVTEKVATVRFTKVYEKERVLRRKKDKLFGEACQFINPETRGCTIYHARPAVCREFPT
                     TKRCAYYDLLTFEREQQDDHDVVPLVKITFRNGKK"
     gene            3343..3561
                     /locus_tag="VFH91_02380"
     CDS             3343..3561
                     /locus_tag="VFH91_02380"
                     /inference="COORDINATES: protein motif:HMM:NF041655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MM0924 family protein"
                     /protein_id="HET6861856.1"
                     /translation="MEDFLAKMVGKTLDVYCGGGSSLRGNVLGLEKGVLLLRDSEGKS
                     CYIAVNKIVVVWEARDDDHRAGFLPNKD"
     gene            complement(3640..4452)
                     /locus_tag="VFH91_02385"
     CDS             complement(3640..4452)
                     /locus_tag="VFH91_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET6861857.1"
                     /translation="MNRFSPQSLLVVRFVTKQSHWMLNGHIPAAKMPLPFGGSPPSPR
                     NKRQVEIEEGEGRSPSMVFRNLSKALFVSFVIFISTTGALAQTRSRLSSQEPDVNDVS
                     CNTDDFTTVALSAEAKPVWEAKTKPIIDLSATTGGSKEAGLLNFQPLLMAAISQRLGS
                     RYRWGATGPYNFDCSGFVWSIYQAVGVSFERSSARTLFGRFSAPLPEEQYKFGTLVFF
                     NGLSHVGVVADENGFYHASRHNGVVYAPFEGYWGKHIDGFRRVPMPLNQLAD"
     gene            complement(4834..>5167)
                     /locus_tag="VFH91_02390"
     CDS             complement(4834..>5167)
                     /locus_tag="VFH91_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861858.1"
                     /translation="NIATLKVKWDPTYQEKVGVATRAAELTPEQHKTLERLSKRIYRY
                     LFLSGYARLDYRMTEDGHFYLLEANPNPQIANKEDFADSAEYSGVPYDQLLQKIMTVG
                     LNYAGRGA"
BASE COUNT         1355 a         1219 c         1333 g         1260 t
ORIGIN      
        1 agccagtttc ctttaatatt tcagccaacc gatggaactt caagtaggac ttacgttagt
       61 actactcatc tggctttcgc tgttggcaac cgtcgacatg gcgtttggtc aattgagcga
      121 cgtaggcttg cgccgcctca tcggtgaaaa cggcgacaaa cccgaacgac gctcacgagg
      181 cttcttaaaa cagatactcg agaatcgtcc gcgatttggc ttcgcgctct ccgccacaat
      241 acagattctg ctcgtcgtcg ttgcggttct catcacctcg atatcattaa ctctgtttcc
      301 tgatccacgt cttgtcttgg tgggacttgc cgcgggccta atcctaaccg gcatttttcg
      361 ccaacttatt ccactcttca tctcgactcg aaatcctgaa ggcacactgc tctttttgct
      421 tccgattgta cggccgttct tccccgtaat gattctcatc tccgatccct ttcaaaggtt
      481 atttgatcga tcacgtaaga gagagattat ttcggacgac actacaaccg aagaagagaa
      541 tgacgatagt gatgatctcc aggcgttaat cgacgtcgga gaagcggaag gcatcctgga
      601 agaggaagag ggtgaactga ttcactctat tattgagttc ggcgacaagc gagtcagtga
      661 ggttatgacg ccaaggcctg acatcgtcgc agtttcaaca acctcgtctg ttcgtgaagc
      721 acgtgacgta atgatcgaat ccaagtattc gcgcctgccc gtctatcgcg aacatatcga
      781 caatgtcgaa ggactaatct atgtccgaga cttacttcaa ttctgggcag aaggccgaga
      841 agatcaaaat attcgaccgc tcatccgttc ggtctatttc atccccgaaa cgaaaccagt
      901 tggcgaactg ctggaggaaa tgcagaaagc gcacgtccaa ctcaccatgg tgatcgacga
      961 gtatggaggc atctgcggcc tggtgactgt tgaagacata ctcgaagaga tcgtgggtga
     1021 aatcgaagat gaagacatcg cgggcgagga actcgttgat attgtcgatg ccggacaggg
     1081 tagctatgac gtactggcat caacagaaat cggcaagata gaacggctct tcgacatgga
     1141 gatcgaggct gatgatttta cgacaatcgc cgggttggtt atcaatgagt cgggcaaagt
     1201 acccagagtc ggagagcggc tgacgatacg gagtctggaa gtggaagtac tcgaggctga
     1261 cgaccgacga attaacaggt taagagtcaa gaaggcacag catgagaacg catcagaggt
     1321 gagcgcgagc taagatggcc aaagttttgc aagtactcac atcggtactc aaaggccagc
     1381 gtccaatcac atgcgagtca tgtggcgagg agttcaattg tggtgcctcg ctgaaaggat
     1441 gttggtgctc gaagattgag ctgagcgaat ctgatcgtgc cgaattaaga tctcgctaca
     1501 aagattgttt atgtggaaac tgtctcgaga gagtagcaac ctccaaagga tccaataaca
     1561 tgtccgatat gctttagctt gtcgtgcgtg ccgaaagtga atcacttgtg ggcaggaacc
     1621 ccgacaagct aaagcatatc ggacatggtc actgtttgaa gtagtgctcg tcggtttcaa
     1681 gcttccgctg aacgcaatgt tttaaggttt aggcaatcaa ctgagcttac aaaacctgca
     1741 aaactgcaaa tccaatggtc aaacataaga gcaaattttt aaaagggcga cgaattgatc
     1801 cagcccctat tacaaaacag attgaggcag ccgatctcat cgacgaatct ttcctcgcct
     1861 acaacgctgc ccggctgcgc gaagcgtgtc acctttttac caaaaaaatg ttggacccgg
     1921 atgttaccgt ggggctttct ctgactggtg ctttgactcc cgcaggactt ggtatttccg
     1981 cgctcatccc tttgatcaag gcgggatttg tcgactggat catttcgacg ggtgcgaatc
     2041 tctatcacga cactcatttt ggcattgggc tcgcgatgca tcagggtaac gctcaaactt
     2101 ctgacatcgt attgcgagag gaagaagtcg tcaggattta cgacatcttc tttgattatt
     2161 ctgtactcct cgacactgac gccttctttc ggaagattat cgaagcgccc gagtttcagc
     2221 gtgctatgtc aactgcagag tttcactatc tatgcgggcg ttatgttcac gaacgcgaac
     2281 agaaactcgg tataagtgaa aagtcgctgc ttggagtggc atacgaatat gcggtgccaa
     2341 tctacacatc ttcccctggc gactcgtcaa tcggcatgaa tgtcgcggcg aaggcacttc
     2401 agggaaacaa actcgctttt gatccgtcga tcgatgttaa cgagacggca tcgattgtcc
     2461 tgtctgccaa acgaggtatt gggagtaaga agaacggtaa gaagcaagcc ggcaagtcgg
     2521 cagtctttat tttgggaggg ggctcaccga agaacttcat gttgcaaact gagcctcaaa
     2581 tccaggaagt gctgggaatc gatgagcgcg gtcatgatta tttcctgcaa gtgaccgatg
     2641 cgaggccaga caccggcggg ctttccggag ccactccggg agaagctgtg tcgtggggca
     2701 aggttgatcc ggatcgttta ccagacgcgg ttgtttgtta tgttgattca acggtggcgc
     2761 taccgttaat aacagcatat gctttaactc gtcacgagcc acgcgaacct aaacgtttgt
     2821 atgatcgtcg cgaagagttt atgaagttgc ttgaaagcga atacaagaaa tcgaaacgac
     2881 gctagaaatc gagggagagc caattcgatc atgagtacca gtcccccaac cgtaaagcca
     2941 aagagctact acgattgcgt tgattgccct gcttattgtt gttccgtata cgaacgcgtt
     3001 caggtcacgc ctcgtgacat tcggcgtctg gcaaaacatt ttgccgtgac agaaaaggtc
     3061 gccacggttc gtttcacaaa agtctatgag aaagaaaggg tgctgcggcg caagaaagac
     3121 aagctttttg gtgaggcgtg ccagtttata aatccggaaa cacgaggttg caccatctac
     3181 cacgctcgtc cggcagtgtg tcgagagttt ccgacaacaa aacgctgtgc ttattacgac
     3241 ttattaacct tcgagcgcga gcaacaagac gatcatgatg ttgtcccgct tgtcaaaata
     3301 acctttcgta acgggaagaa gtgaacccgg ggaattatta ctatggaaga ctttctggca
     3361 aaaatggttg gcaagacttt ggatgtttac tgcggcggtg gttcgagtct tcgtggcaac
     3421 gtactgggac tcgaaaaggg ggttctgctc ttacgggaca gtgagggcaa gagctgttat
     3481 atcgcagtaa ataaaattgt ggtggtgtgg gaggcgcgcg atgacgatca ccgcgcgggc
     3541 ttcttgccaa acaaggactg acttaaaaaa gtttcaaaac aaaacgccgg ccgctccttg
     3601 aatgagcgcc ggcgtttgtc atctcgaaag cacgaaaagc taatctgcaa gctgattcag
     3661 gggcatcggc actcgacgga agccatcgat gtgttttccc cagtaacctt caaaaggcgc
     3721 gtagacaacg ccgttgtgtc gcgaagcgtg gtagaagcca ttctcatcag cgacgacgcc
     3781 aacgtggctt aacccattaa agaaaacgag agttccaaac ttgtattgct cttctggaag
     3841 aggagccgag aaccgaccaa agagggtgcg tgcgctggac cgttcaaagc tgacaccgac
     3901 tgcctggtag atgctccaga caaagccgga acagtcaaag ttgtatggac cagttgctcc
     3961 ccagcggtat cgagaaccca accgctgaga aattgcggcc atcaacaaag gttggaagtt
     4021 taaaaggcct gcttcctttg aaccacctgt tgtcgccgat aaatcgatta ttggttttgt
     4081 ttttgcttcc caaactggct tcgcttccgc cgataacgct acggtcgtaa agtcgtctgt
     4141 gttacaggaa acatcgttca cgtcgggttc ttgcgatgaa agccttgaac gagtctgcgc
     4201 cagcgcccca gtggtgctga taaaaataac gaacgaaacg aacagggctt tagataagtt
     4261 acgaaatacc atagacgggg aacgaccttc tccttcttca atttctactt gtcttttatt
     4321 gcgtggtgag gggggactac cgccgaaagg caaaggcatc ttagccgctg ggatgtgacc
     4381 attcaacatc caatgtgact gtttcgtgac gaaacgcacc accagtaacg attgcggact
     4441 gaatctattc acgaaatctt gtcaaattat tgcaacgata acagtcgcat ctgctcagtg
     4501 tgcgatcggg aaacagctag cagtcaatat gaaggaattg acgtccctga gttgctaggg
     4561 ccaacccccc tgtgccgcaa tcgagcttga gacctcaccg gcgtttaacg taaacagtgc
     4621 agagtaagcc aattagggca ctgccgattt atcgtatggc ttgacctttt ggtcaggctt
     4681 acgcccgctg cattaatcga tagagattca gagtaaaaag tacagtaaga gctatagtca
     4741 tggggcatcg ctccagaccc gaatcagatt atttgtatgc ttgtatttaa acggcgaatc
     4801 ggaaccgggt tccgaaatga agttcgttcg atgctaggca ccacgacccg catagttgag
     4861 gccaacggtc atgatcttct gaaggagctg gtcataaggc acgccactgt actcggccga
     4921 gtctgcaaaa tcctctttgt tagcaatctg gggattcgga tttgcctcaa gtagatagaa
     4981 gtgaccatct tcagtcatgc gatagtcgag gcgtgcgtat ccacttaaaa acagataccg
     5041 atatattctt tttgacagtc gctcaagagt tttatgttgc tcgggggtaa gttcggcggc
     5101 gcgggtcgca acaccaacct tctcctgata agtaggatcc catttaactt tcagagtggc
     5161 gatgttc
//
LOCUS       DASSFP010000112         4213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_470286, whole genome shotgun sequence.
ACCESSION   DASSFP010000112 DASSFP010000000
VERSION     DASSFP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470286"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..459
                     /locus_tag="VFH91_02395"
     CDS             265..459
                     /locus_tag="VFH91_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861859.1"
                     /translation="MANKNSKSHRELIDTGKDKRFVRRNEQGEFDNVTDVGKSIAQDR
                     KQSAKRKVPSGQGDRGDQKR"
     gene            complement(483..689)
                     /locus_tag="VFH91_02400"
     CDS             complement(483..689)
                     /locus_tag="VFH91_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861860.1"
                     /translation="MSRTKKAVNEPVKTRDKKFLVVCSSCRTVIRTSTTDLGERMCLI
                     CHARMLNDYFRRIGGASLKTRRRQ"
     gene            773..1792
                     /locus_tag="VFH91_02405"
     CDS             773..1792
                     /locus_tag="VFH91_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861861.1"
                     /translation="MTKIERLQSIGIRRLGSTQRGFRYRSSTGGRVAKTDLERIETLK
                     IPPAWKEVAINPSANGRVQVIGKDAAGRWQYLYHPSHTRMQEARKFKRLTKFALTLPA
                     MRNTVSRHLRQKDLGRERVLACILRILSTCFLRPGSQVYASENGSYGIATLRRKHVRV
                     KGSTVEFDFPGKSGVQQQRKLEDGQVAKVIRALLKEPGHNVFKYRNGDGALTKVTGRQ
                     INAYIKEVMGEKFSAKDFRTWAGTLVCASALAREGLEEDENPRTVKKKIVAAVKETSE
                     ALGNTPAVCRSSYVSPEILHAFEQGKVIDSYFNDLGDLTSFRGRSFHKSERALLKFLK
                     RSVTK"
     gene            complement(2194..3264)
                     /locus_tag="VFH91_02410"
     CDS             complement(2194..3264)
                     /locus_tag="VFH91_02410"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6861862.1"
                     /translation="MKFLEQFFRHDLAIDLGTVNTLIYDPDKGIILNQPSVVAIDRYD
                     GEVLAVGHQALKLLGREPRGIEVHRPIRGGTIFNFEVTQKMLRTFISRVTDGYRRGHF
                     VVGVPGSATNVEQRSVRDAARDAGGSRIDLVDEGLAAGLGAGVSFDDERAHLVVDIGG
                     GTSNIAIIASGGVVSSISLAAAGNAMDESIRDYIRSNYFLHIGEYTAETIKKELGTLV
                     SDDSSQDERRLEVVGKHLTDGTACAVQVSTNDVRLALEPILAEIVTGVRRVIEEAQPE
                     AVADIYYSGVILSGGGSLLKGLKERLQQELGLHVTMPEDPMTTVVMGAGALMAKPDML
                     YRCAIRQNLPVWQASEELVATW"
     gene            complement(3522..>4213)
                     /locus_tag="VFH91_02415"
     CDS             complement(3522..>4213)
                     /locus_tag="VFH91_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861863.1"
                     /translation="EFFTWEFATACAGWSIGINPFDQPNVQESKDATKHLLQVFKDQG
                     QVPQQKKLVADHSLTLYVEDDGSQTVSSGSVMQAISALLQSVKPNDYIAFLVYTEETP
                     RITEILAAIRMDILAVTRCATTIGFGPRFLHSTGQFHKGGPDTGVFFQLTADDQVDFP
                     VPGEPYSFSVLKQAQALGDFQSLAKHGRRAIRVDLGNQIEEALRSLREEIRKALSDFP
                     VQGLSEAKSVS"
BASE COUNT         1090 a         1049 c         1051 g         1023 t
ORIGIN      
        1 gtttcaaagc caaaaaatca gtcccaaggt cagctaatta cttgaatttg attccgccag
       61 ctaactgact ggtctcaccc actctccagc aattactgcg cccaccaccg gattcagagc
      121 gtctactcat ttttcgttga cctgatggca tttatcttgc ggtgcgagga aagaaggagg
      181 ggatcaaaag ctctcttgtg ctcagaatgt aaacaaaccc ggcgtgtgca tgtaccggaa
      241 gactacacat tagggaggtt ataaatggcc aacaaaaata gtaagagcca tcgcgaactc
      301 atcgataccg gtaaagacaa acgttttgtc cggcgaaacg aacaagggga attcgataac
      361 gtcaccgacg ttggaaaatc aattgcgcaa gaccgaaagc agagtgcaaa gcgtaaagtt
      421 ccatctggac aaggtgaccg gggtgatcaa aaacgataag cttcgcagtc attttcccga
      481 cgctactggc ggcgacgagt tttcaggctc gcgccgccaa ttcgtcgaaa gtagtcattc
      541 agcattcgag catggcaaat caaacacatt cgttcgccca agtcagtcgt actggttctg
      601 atgacagtgc gacacgaaga gcaaactaca aggaacttct tgtctctggt tttgacgggt
      661 tcgtttactg cttttttggt gcgactcaat cgatatctct ttctcaaaaa ctagacttga
      721 atgactgcac aacaatttcc gttccataaa ttcaagacta agaattcatt acatgacaaa
      781 gattgaacga ctgcagagca tcgggattcg tcgacttggt tcaactcaga gaggttttcg
      841 atatcgatca tcaaccggag gacgagtcgc taaaacagat ttggaaagaa tcgaaacctt
      901 aaagatccct ccagcgtgga aagaagtcgc aatcaatccg agcgcaaacg gtcgtgttca
      961 agtgattggt aaggacgccg ccggacgttg gcagtacctt tatcatccca gtcacacacg
     1021 catgcaagaa gcgagaaagt tcaaacgact tacaaagttt gcgctgacac ttccggcaat
     1081 gagaaacact gtctctcgac acttacggca gaaggatttg ggacgtgagc gtgtactcgc
     1141 atgcattttg aggattcttt cgacatgctt tttgcgaccc ggtagtcagg tgtatgcgag
     1201 tgaaaatggc agttatggaa tcgcgacgct gagacggaaa cacgttcggg tcaaaggcag
     1261 cacagtcgaa tttgattttc caggcaagtc gggcgtccaa caacaacgca aacttgaaga
     1321 cggccaggtg gcaaaagtaa ttcgcgcact actcaaagag cctggccaca acgtattcaa
     1381 gtatcgcaat ggcgacggtg cattgacgaa agtgacgggc cggcaaatca atgcttacat
     1441 aaaggaagta atgggcgaga agtttagcgc caaggacttt aggacctggg ccggcactct
     1501 ggtctgcgct tcggccctcg cccgcgaagg cttggaggaa gatgaaaacc caagaacggt
     1561 aaagaaaaag atcgtggcgg cagttaaaga aacttcggaa gcacttggaa atactccggc
     1621 tgtttgccga agctcatacg tatcacctga aattctacat gcgttcgaac agggtaaggt
     1681 gatcgatagt tacttcaatg atcttggcga cttaacctcg ttcagaggaa gaagctttca
     1741 caaatctgaa cgagcactcc tgaaatttct caagcgttca gtaacaaagt aacagctgac
     1801 gtttcgtgcc tggcaccatg tttgccattc ggatactaaa gcttttcttt gttcggattt
     1861 agtgcgaacg aattttgatt ttgcaggagg cagaaaatgc aaggaccatt acgggggata
     1921 ttggcaggag cggcagccta taagtttggc ggcggatgtc tctcgatgat actaattttt
     1981 gtaatcgtat tttggctgct tggatatgtt cgatgctaat ggctatcgat tctcgaacga
     2041 tgttggtcca gctgcaacta ttcgatgaac aacgtaatgg tgtttaactc gagttgcatc
     2101 ttcgcccacc atccagggcc caattagcgt agcgcggtgg cgtcaataat cacatcgacg
     2161 gcaccgcacc tcgttatcaa ccgtggccag ctgttaccag gtagctacca actcttcaga
     2221 ggcttgccac acgggcaggt tctgacgaat agcgcatcga tacaacatgt ctggtttcgc
     2281 catgagcgct ccggcgccca tcaccacggt ggtcattgga tcctctggca tcgtcacatg
     2341 aagtcccagc tcctgctgga gacgttcttt taatcctttc aacaacgatc caccgccgga
     2401 aaggatgact ccggagtaat agatatctgc gactgcttca ggttgcgcct cttcaataac
     2461 gcgccttaca cctgtgacga tttcagccaa tattggctcc agcgccaaac gcacatcgtt
     2521 cgtagagact tgcactgcac aggctgtacc gtcagtaagg tgtttaccta cgacttccaa
     2581 tcgtcgctcg tcttgagacg aatcgtcgct cacgagcgta cccagctcct tcttgatagt
     2641 ctcagctgta tattcaccaa tgtgcaggaa atagtttgag cgaatgtagt ctcggatgct
     2701 ttcatccatc gcatttccgg cagctgccag gctaatggac gacacgacac cgcccgaagc
     2761 aataatcgca atgttgctag taccaccccc gatgtccact acgaggtgtg ctcgttcatc
     2821 atcgaaactc acgcccgcac ctaagccagc ggcgaggccc tcgtccacga gatctatgcg
     2881 gctgcccccg gcatctctag ctgcatcgcg gaccgaacgc tgttcaacgt ttgtggccga
     2941 accagggaca ccaacgacaa agtgtcccct tctgtaccca tcggtgacgc gactaatgaa
     3001 cgtccgcaac atcttttgag ttacttcaaa gttgaagatt gtgccgcctc tgatgggacg
     3061 atgaacttct atcccgcgtg gttcccgacc gaggagtttt agcgcctgat gtcccacggc
     3121 caaaacctct ccatcgtacc gatcaatggc aaccactgaa ggctgattga ggatgatccc
     3181 cttgtcggga tcgtagatta gtgtgttgac agtaccgaga tctatcgcta gatcgtgcct
     3241 aaagaattgc tccaagaact tcatcgcgac gattcctcct ccagatgggc agggttcagg
     3301 gggtgcgagt gacgaagaac aagtggcaac tcgcatgcct gaggtgtacg tcgtagggga
     3361 ttcctttaag gtaacagaga gccattacat ctctgtttcc agaaacgtac gggagctatg
     3421 cgcacggggc gtgcacaact gcatggctct cggtcgttca tgatgactga gttgcttttg
     3481 gacggggcta ggatgtctca gccggaatgg aagttttagt tttatgagac tgacttagcc
     3541 tctgaaaggc cctgcacagg gaaatctgaa agcgctttgc gaatctcttc acgaagactg
     3601 cgcagtgcct cctctatttg attaccgaga tcgacccgta tcgctcgtct gccgtgttta
     3661 gccagtgact ggaaatctcc taaggcctgc gcctgtttta ggacactaaa gctgtacggt
     3721 tcgccaggca ctgggaaatc aacctgatcg tctgcagtga gttggaaaaa tactcctgtg
     3781 tcgggccctc ctttatgaaa ttgccccgtt gagtgaagga agcgaggtcc aaaaccgatt
     3841 gtggtggcgc accttgtgac cgccaggata tccattctaa tggctgcgag aatctctgtg
     3901 atccggggtg tttcttccgt atagacgaga aaggctatgt agtcatttgg tttcacagat
     3961 tggagtaaag cgctgatcgc ctgcattaca gatccgctcg aaactgtttg cgatccatca
     4021 tcttcgacgt aaagcgtcaa cgaatgatca gctacaagtt tcttctgctg cggaacttgc
     4081 ccctggtcct taaagacctg gagcaggtgc ttagtagcgt ctttcgactc ttgtacgttc
     4141 ggctgatcga agggatttat tcctatggac caacctgcac aggcagtcgc aaactcccag
     4201 gtaaaaaact cgg
//
LOCUS       DASSFP010000113         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1066559, whole genome shotgun sequence.
ACCESSION   DASSFP010000113 DASSFP010000000
VERSION     DASSFP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066559"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2523)
                     /locus_tag="VFH91_02420"
     CDS             complement(<1..>2523)
                     /locus_tag="VFH91_02420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6861864.1"
                     /translation="RKRAAGSLAQQAAAMKASMDGMAIIDHSGLCIYLNDAYAKVFGY
                     TDPQRLIEASWEMFYFEEELHRLKKEIMPAVWRDGSWRGEAMGLGLNGSSIPLEISIS
                     SVDGGGLVQVVRDITERKKAEETLRNSSLKDDLTGLYNRRGLLKHAAPYFDFASRQKE
                     ALLLLFIDLDGMKRINDEFGHNEGDNALVATAQILNRSFRNSDIIARLGGDEFAIVVT
                     DLNAKKEDAIARLTNNLKSYNASAQCRHKLSFSIGVAALEPQRMTCFEELLEQADQAM
                     YEQKRMKKRRESERIQNKQLGASEVDPNAALSSSIPFALSATPQASKGSYGTFDNAAI
                     GMAVVSVDGSWLQVNEALCKLLGYSEQELRATSFQRLTHNEDLKQVQSYIQRVIEGYI
                     QSHEQEKRYIHEQGHTVWVQWHVSLLKDSESGVKRLFFQVQDISDRKKAEEKLTQDTL
                     TGLPNRARFNDLVKLRVARKQSNKDQKFAVLLLDVDRFKLVNDSLGNSSGDQLLMQIA
                     HRVSTCMRQGDVLGRVGGDEFAVLLDDVTGEEEACSVATRIQQALAISFNLFGQEVYT
                     TMSIGIAMGTGYSEQVSDMLRDAETAMHRAKECGKARYEVFGRDMHGELMSRLKMETD
                     LRHACERGELFVDYQPIVSLANRTLMGFEALVRWRHPECGLIPPKDFIPVAEETGQIL
                     AIGQTVLESACRQAREWQITYPVKPPLFVSVNLSVKQFNQPGLVDNIAQLLEEFDLPP
                     RCLKLEITESVFSDNIEAAVGLLTQLRNLGVQLSIDDFGTGYSSLSYLQRFPIDTLKI
                     DRSFVTQMMENEENIAIVRTIVALAQNLGMDVVAEGVETEDQV"
BASE COUNT          575 a          645 c          635 g          668 t
ORIGIN      
        1 acctgatctt ccgtttcgac gccctctgct actacatcca ttccgagatt ttgagcgagg
       61 gcaacaatcg tgcggacgat tgcgatattt tcttcgttct ccatcatctg agtcacgaat
      121 gacctgtcga tctttaaagt atcgatagga aatctttgca ggtagctcaa gctcgagtag
      181 ccggttccga aatcatcgat actcaattga acaccgagat tcctcagctg cgttaataga
      241 ccaaccgcgg cttcgatgtt gtcgctgaaa acagattcgg ttatctccag tttaagacag
      301 cgaggaggta gatcaaattc ctctagcagc tgggcgatgt tatcaactaa tcccggttga
      361 ttgaactgct tcaccgacaa gttcacgctt acaaacagtg gtggtttaac gggataagtg
      421 atttgccact ctcgagcctg gcgacaagcc gattcaagga ccgtctgacc aattgccaga
      481 atctggccag tctcttcggc gacgggaatg aaatcctttg gcggtatgag gccacattcc
      541 ggatgtctcc atctcacaag tgcctcgaag cccatgagag tacgattggc aagagagacg
      601 attggttgat agtcaacgaa tagttcgccg cgttcacagg catgtctcag atcggtctcc
      661 atcttcaggc ggctcatcaa ttcgccgtgc atgtcgcggc caaaaacttc gtatcgtgcc
      721 ttgccgcatt ccttagcacg gtgcatagcg gtttcagcgt cacgtaacat atccgagacc
      781 tgttcggaat atccggttcc catcgcgatg ccaatactca tcgttgtgta gacttcttgt
      841 ccgaacaggt tgaatgaaat tgcgagcgcc tgttggatac gagtcgcaac agaacaagcc
      901 tcttcttcac ccgtaacgtc atccagtaga accgcgaact cgtctccacc gacgcgacct
      961 agtacgtcac cctgacgcat acacgtgcta acgcgatgag cgatttgcat taagagttga
     1021 tcgccgctcg aattgccgag actgtcattt accagtttaa agcggtcaac gtccaggaga
     1081 aggacggcaa atttttgatc cttattggat tgctttcggg ccacgcgcag tttgacgaga
     1141 tcattaaacc tggcgcggtt gggcagtcct gtgagcgtgt cttgcgtcaa tttctcttcg
     1201 gctttcttgc ggtcgctaat atcctgaacc tggaagaata gacgcttcac acctgattca
     1261 gaatccttga gcagcgacac atgccattgg acccagaccg tgtgaccttg ctcatgaatg
     1321 tatctcttct cctgttcgtg actttgtatg tagccttcga tcacgcgctg aatgtatgac
     1381 tgaacttgtt tcaagtcctc gttatgtgtc aggcgctgga aacttgttgc gcgcaattcc
     1441 tgctcggaat atcccaggag cttgcaaagt gcttcgttga cttgaagcca actgccatcg
     1501 actgacacca cggccattcc gatcgcggca ttatcgaatg ttccgtaact tcctttagat
     1561 gcctgcggag ttgcggaaag cgcaaacggt attgaactgg aaagagctgc gttagggtcc
     1621 acttctgacg caccaagttg tttgttttgg atgcgttcag attctctccg cttcttcatg
     1681 cgcttctgtt cgtacatggc ctgatccgct tgttccaaca actcttcgaa gcaggtcatg
     1741 cgttggggct cgagagcggc gacaccaata ctgaatgaga gtttgtgtcg gcactgtgcg
     1801 ctggcgttgt aagacttgag attgttagtg agtcgagcaa tagcgtcttc ttttttggcg
     1861 ttcagatcgg tgacgacaat cgcaaactcg tcgccgccga ggcgggcgat gatgtcagag
     1921 ttgcgaaaac ttctattgag tatctgggca gttgcaacca gagcgttgtc gccttcgttg
     1981 tgtccgaatt catcgttaat acgcttcatc ccgtcgagat cgataaacag aagtagcagc
     2041 gcctcttttt gacggctggc aaagtcgaaa tagggtgcgg cgtgttttaa taaaccgcga
     2101 cgattataga gaccggttag atcatcctta agggaactat tacggagcgt ttcttcagcc
     2161 ttcttgcgct cggtaatgtc tcggacaacc tggaccaatc cgccgccgtc gacacttgag
     2221 atggaaatct cgaggggtat gctactgcca ttcaagccta accccatcgc ttcaccgcgc
     2281 caggaaccat cacgccagac tgcaggcatg atctctttct ttagacgatg gagctcttcc
     2341 tcgaagtaga acatttccca gctggcttcg atcaagcgtt gagggtcggt atagccgaat
     2401 acttttgcat acgcgtcgtt gagataaata cagaggccgc tatggtcgat aatggccatg
     2461 ccgtccatcg aggctttcat ggcggcagct tgttgggcga gagagccggc ggcgcgttta
     2521 cgt
//
LOCUS       DASSFP010000114         4931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_918152, whole genome shotgun sequence.
ACCESSION   DASSFP010000114 DASSFP010000000
VERSION     DASSFP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918152"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2524
                     /locus_tag="VFH91_02425"
     CDS             <1..2524
                     /locus_tag="VFH91_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6861865.1"
                     /translation="PIRLRDVGRVEDGTKEQRSLARLNGTPTVTLDVRRQSGANTVEV
                     IKGIKDALPRVTAQLPQDVKLVVIRDQSRYIEAALHEIQTHLILGSILASLVVLVFMR
                     SWRSTLIAAVAIPCSVISTFGMMKALNFTLNSVTMLALVLMVGVVIDDAIVVLENIFR
                     FIEEKRIEAKEAAREATKDIGLAVLATTLSLVVIFLPVSFMSSISGRFLYQFGITAAV
                     AIMVSLLVSFTLTPMMSSRLIRVADAKGGHEAPSSRKGFYRWIDLGYSKLLAFAMRHR
                     LAVAVLAFGTVLTCIPLYRAVKQEFIPTNVDEAEFEVSVNGPEGTNLAVMNETITAME
                     RDILATPGVNMVLSSAGGSFLGGVNQGGAYVRIAPHEERTLSFTKFWNEAKKGHPFNV
                     FRGNYTQQDVMAEVRRVLQRYAPMRISVRNAQSFNFGAGGRQDIDFVFRGPDLVALSG
                     YADDLLARSQTLGGIVDGDTTLKLNKPELRVVIDRDRAADLGVDTSDIANSLRLMVGG
                     EDQASRFRDDATNEDYDVQLRLTEADRNDPSTLSHLYVPSSRGQLVRLDNLVKIKRDT
                     SPSRIDRLDRERQVSVRASVAPGYALADRIEALRGAVAAMNLPTAYTTTVSGRARELE
                     RTFTEFIWAFILSIIFMYMILASQFESTIHPLTILLSLPLSVPFALLSLWGTGDTLNL
                     YSALGILVLFGVVKKNAILQIDHMNNLRAKGMERYDAIMQGNRDRLRPILMTTLALVA
                     GMLPLALGTGPGAEERRSIAVVVIGGQSLSLLLTLIATPVVYSLLDDMRATGRLRTLA
                     TTISGLRKGIGEKRKRRRPAREATAERAPVETTEDSIPTAGD"
     gene            complement(2534..3310)
                     /locus_tag="VFH91_02430"
     CDS             complement(2534..3310)
                     /locus_tag="VFH91_02430"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET6861866.1"
                     /translation="MSAPQKADQTEIRKLVSPEEWQVRVDLAAAYRLVADFGWDDLVF
                     THISARVPGPEHHFLINPYGMMFEEITASSLVKVDLEGNIVMDSEYHINPAGFTIHSA
                     VHAAREDALCVMHLHTDCGIAVSTQENGLLPISQQALFVLASLGYHDYEGLALNDDEK
                     PRLVADLGRKTFLILRNHGLLTVGPTVSDAFLSMFLLERACRIQILAQSGGGKLIPIP
                     SSVLAQIPAQEAVVTKGGRGQLAWPGLLRKLDRLGANYNE"
     gene            complement(3321..4778)
                     /locus_tag="VFH91_02435"
     CDS             complement(3321..4778)
                     /locus_tag="VFH91_02435"
                     /inference="COORDINATES: protein motif:HMM:NF016021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861867.1"
                     /translation="MGTLNIDRYHAAMGDASFKDVTHLHNQYLCDAEATFYITQRKLP
                     FAPCLGHERLIRLLVDSQIDRPRLRFLEQDRGGLKLFAKAIEDLQFVGNIRAVKPGTI
                     VFAHEPFADITGAFGLTQAQEIKFEHAFDLPMTTASLAMQFRMAAGDRWLSDFSLRRN
                     GDIERAVDIAVYAFIGGFNDTSNMEAAHRLDIPAVGTEAHYWQQSFIEYMYDPEIEPR
                     TGKPKHFEQVAFERWLDANPNGTTLLLDTIDVYMGAVHAAMAATSTDARRKAFKGFRV
                     DSGDLAELGEWCFRFFEANGLRGLRANLTGDLDVEKIKRIVEEFPEVGGFGIGTKLSS
                     EVKTVAGVIFKQCLMKGRPTLKASNSQDKITLPGNLQLFRGIDKNGTYIGDVIGLEEE
                     DVQMDNAVQVERLLAPFWENGQHSAIPSIMKQKAFVEEQRKRFADISSYPVSLSKRLR
                     QLRDRLVEETRADNSGWQEILNLPDATVVESLQSK"
BASE COUNT         1151 a         1279 c         1266 g         1235 t
ORIGIN      
        1 gccaatcaga cttcgtgacg tgggacgagt agaagatgga acaaaagagc agcgttcttt
       61 agctcgccta aatggaacgc ctacggtaac gctcgatgtc agacggcagt cgggagccaa
      121 tactgtagaa gttattaagg gtataaaaga cgcgcttcca cgcgttacgg cccaactgcc
      181 acaggacgtg aaactcgttg ttattcggga ccaatccagg tacatcgagg ctgcacttca
      241 cgaaattcaa acccacctca tactcggaag tattctcgcc agtctggttg ttcttgtctt
      301 catgcgctcg tggcgctcaa cgttgattgc agcggtggcg attccgtgtt cggtcatttc
      361 aacatttggg atgatgaagg cgctgaactt tactttgaac agcgtcacca tgttggcctt
      421 agtgttgatg gtgggcgtag ttatcgacga cgctatcgtt gtacttgaga acattttccg
      481 ctttatcgaa gagaaacgga tcgaagctaa agaagccgcg cgagaagcta caaaagacat
      541 tggacttgcc gttctggcta caactctgtc tctggtcgtc atctttctcc ctgtctcatt
      601 catgtcttcc atttcggggc gctttctgta ccagtttggt attactgctg cagttgccat
      661 catggtcagc ttgttagttt cattcacgct gacacctatg atgagctctc gcttgatacg
      721 agtggcagat gcgaaaggtg gtcacgaggc tccttcatcc cgaaagggtt tctatcgttg
      781 gatagatctc gggtattcaa agctgttggc ctttgcaatg cggcaccgcc tcgcagtcgc
      841 agttcttgcc ttcggcaccg tgttgacttg tattccttta tatcgcgccg tgaagcagga
      901 gtttattcct accaacgtcg acgaagcgga gtttgaagtt agcgttaacg gtccagaagg
      961 aacaaatctc gcagtgatga atgaaacgat cactgcgatg gagagagaca tactcgcaac
     1021 gccaggcgtg aacatggttt tgtcgtctgc cggcggaagc tttctcggtg gtgtaaacca
     1081 gggcggagcg tacgttcgga ttgcgccgca tgaagagcgc acattgtcgt tcacaaagtt
     1141 ctggaatgaa gcaaagaagg gtcatccttt caatgtattt cgcggcaact acacacaaca
     1201 ggatgtgatg gccgaagtga ggcgcgtgct gcagcgttac gcgcccatgc gcatcagcgt
     1261 gcgcaatgct cagtctttta actttggggc cggcggccgc caggatattg actttgtgtt
     1321 tcgtggtcct gatctggtgg ccttatctgg ttatgcagat gacctgttgg ctcggtctca
     1381 gacgctcgga ggaattgttg acggcgacac aaccctcaaa ctgaacaaac ctgaactgcg
     1441 agttgtcatc gaccgcgacc gggcagctga cctgggcgtc gatacctctg acatcgcgaa
     1501 cagtttacga ttgatggttg gcggcgagga ccaggcctcg cgtttcagag atgacgctac
     1561 aaacgaagat tacgatgttc agcttaggct aaccgaagct gatcgtaatg acccttcgac
     1621 tttgagtcat ctgtacgtac cttcatcgag gggccagctg gtccggctcg acaaccttgt
     1681 caaaatcaaa cgtgatacca gtccttctcg aatcgatcgt ctcgatcgcg aaagacaggt
     1741 ctctgtgcga gcctcagttg ctcctggtta tgcacttgca gatcgaatcg aagcgttgcg
     1801 cggcgcagtt gcggcgatga atctaccgac agcctacaca acaacggtct cgggtcgtgc
     1861 gcgtgagctc gaaaggacgt tcactgaatt catctgggca ttcatcctgt cgatcatctt
     1921 catgtacatg atcctcgcct cgcaattcga aagcacgatt catccgttga cgatcttgct
     1981 cagtcttccg ctctcggttc cttttgctct tttatcactc tggggaaccg gagatacgct
     2041 caatctctat tcagcactcg gtattcttgt gttgtttgga gtggtgaaga agaacgcgat
     2101 cttgcagatc gaccacatga acaatctccg tgcgaaaggt atggagcgtt atgatgcgat
     2161 catgcagggg aatcgagatc ggctacgccc aattttgatg acgacgcttg cgctggttgc
     2221 cggtatgttg cccctcgcgc tcggaacagg tcccggcgcc gaagaaaggc gttcaattgc
     2281 agtggtggta attggtggac aatcgctgtc gctcttattg acgttgattg ctacgccggt
     2341 tgtttattct cttctcgatg acatgagggc cacggggcgc ttgcgcaccc ttgcgacaac
     2401 catctcggga cttcgaaagg gaataggcga gaaacgtaaa cgccgacgtc cggcgcgaga
     2461 agcgaccgct gaaagagcgc ctgttgagac aaccgaagat tcgattccca ccgcgggcga
     2521 ctgattgttt cagttactcg ttatagttcg cacccaatcg atccagctta cgcaagaggc
     2581 cgggccacgc cagttgccct ctaccacctt tagtcaccac tgcctcctga gcaggaatct
     2641 gggcgagtac tgacgacgga attggaatga gtttgcctcc gcccgattga gccagaatct
     2701 gaatgcgaca tgctcgttcg agcaggaaca tcgacaaaaa cgcgtccgaa acggttggac
     2761 caacggtgag cagtccgtga ttccgcaaga tcaagaacgt ctttcttcca agatcggcaa
     2821 ctagcctcgg cttctcgtcg tcattaagcg ccaggccctc gtaatcgtga tagcccaacg
     2881 acgcaagtac gaagagagct tgttgcgaaa ttggcagtaa tccattttcc tgcgttgaga
     2941 ccgcgatccc gcaatcggtg tgtaagtgca tcacgcagag cgcatcttct cgcgcagcat
     3001 gcacggcgct atggatcgta aagcccgcag gattaatgtg gtactccgaa tccataacga
     3061 tgtttccctc gagatccacc tttactaaac tggaagcagt gatctcttca aacatcatcc
     3121 cgtagggatt aattaggaag tggtgttccg gtccaggcac tcgcgccgat atgtgagtga
     3181 atacgagatc atcccacccg aagtcggcca ctaaccgata tgccgccgcg agatccaccc
     3241 gcacttgcca ctcttcaggt gacactagct tacgaatttc tgtctgatca gctttttgag
     3301 gtgcgctcat gagtgtcctt tcatttcgac tgcaaactct cgaccacggt tgcgtccggt
     3361 agattcaata tttcctgcca acccgaatta tcggctcgcg tttcctcaac tagccgatca
     3421 cgcaactggc gcagccgctt actcagggaa acaggataac tggatatatc agcgaagcgc
     3481 tttctttgtt cctcgacgaa tgccttctgc ttcatgatcg acggtattgc ggagtgttga
     3541 ccgttttccc agaacggtgc aagcagcctt tcaacctgga ccgcgttatc catctgcaca
     3601 tcttcctctt ctaaaccaat gacatcgcca atgtaggttc cgtttttatc aatgccgcgg
     3661 aatagctgaa gattgccagg taaggtaatt ttatcctgag agttcgaagc tttaagggtt
     3721 ggcctacctt tcatcaagca ttgtttgaaa ataacgccgg caacggtttt gacttcactg
     3781 gaaagttttg ttccgatgcc gaaaccgccc acttcgggaa actcttcgac aatccgtttg
     3841 atcttctcga cgtccaggtc gccggtaagg ttcgcacgca gcccacgcaa tccattggct
     3901 tcaaaaaagc gaaagcacca ctcaccaagt tcagctaagt cgccagaatc aacgcgaaag
     3961 cccttgaaag cttttcggcg agcgtcagtt gaggttgccg ccatggcagc atgaactgcg
     4021 cccatgtaga catcgatcgt gtccagtagc agtgttgttc cattgggatt ggcatccagc
     4081 caccgctcaa aagctacctg ttcaaaatgt tttggttttc cggtgcgcgg ctcaatttcc
     4141 ggatcgtaca tgtactcgat gaatgattgt tgccagtagt gagcttcagt tccgactgcg
     4201 ggaatatcga gtcgatgagc agcctccatg tttgacgtat cgttgaatcc tccaatgaat
     4261 gcgtaaacag cgatatcaac tgctcgctcg atgtctccat ttctgcgcaa agagaaatcc
     4321 gatagccaac gatcgccagc ggccatacgg aattgcatcg ccaaactggc ggtagtcata
     4381 ggcaagtcga aagcgtgctc aaacttaatt tcctgtgcct gggttagacc aaacgctccc
     4441 gtaatgtcgg cgaatggctc atgagcgaag acgattgtgc cgggttttac agctctgata
     4501 tttccgacaa attgaaggtc ctcaatcgct ttggcaaaga gtttcagacc gccgcggtcc
     4561 tgttccagaa accgtagccg aggtcgatca atctggctat ctacgagcag ccgaatcaaa
     4621 cgttcatgac cgaggcacgg agcaaacggt aacttgcgtt gtgttatata aaacgtcgcc
     4681 tcggcgtcgc agagatactg attgtgcaag tgggtcacat ccttaaagga cgcgtcgccc
     4741 atcgccgcat gatatcgatc gatattgaga gttcccatga agtgctacaa aggtaaaagt
     4801 cagtcaatag cggattgaaa ggtggcttcc acggcaagac taattccgat attgtttttc
     4861 gacaagagcg ccggcggctt gcatctcttg gagtgcacgg tctccgtcac ccggttccat
     4921 atcaaccggc c
//
LOCUS       DASSFP010000115         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_918832, whole genome shotgun sequence.
ACCESSION   DASSFP010000115 DASSFP010000000
VERSION     DASSFP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918832"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFH91_02440"
     CDS             <1..186
                     /locus_tag="VFH91_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861868.1"
                     /translation="NRRSIIILIRGTLCTSFTFGSTSRSVAALRGFGLGERQSGSIGK
                     KYQAAEWRAHSDLRLQL"
     gene            complement(163..1359)
                     /locus_tag="VFH91_02445"
     CDS             complement(163..1359)
                     /locus_tag="VFH91_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6861869.1"
                     /translation="MTTPATENRRVSKKAKSFTESVIREMTREALKYGAVNLSQGFPD
                     FAAPEDIKRRAMEAIANDVNQYAITWGSKDFRDAIARKTSWYLGLEIDPETEITVTCG
                     STEGMIAAMMATVDPGEEVIVFEPYYENYAPDAILSDARPRYVPLREPDWSFDRDELR
                     SAFNSNTKAIIICNPNNPTGKVFTRDELEYIAGLCREFDALCFTDEIYEHILYPREGA
                     EIAHISMAQLEGMRERTVVVNSMSKTYSVTGWRVGYCIAPPEITSGIRKVHDFLTVGA
                     AAPLQAAGAYALSLPGEYYDALQDEYRARRDRLLPVLESAGFRAFVPDGAYYVMTDIS
                     SFGFPNDIEFTKHLIREIGVACVPGSSFYSGSNRGQQQVRFCFCKKDETLNRAAERLL
                     KLQAKI"
     gene            1481..2743
                     /locus_tag="VFH91_02450"
     CDS             1481..2743
                     /locus_tag="VFH91_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET6861870.1"
                     /translation="MKSKICSTLCLHFLLAISLSPSISNAQVNRGKMSGPGLENAKAL
                     AHFYESLEQVKAGSRLEPVRVMHFGDSHIAADVLTRSIRERLQSDFGDGGPGFIVPGN
                     PMATKRAGVISGATEGWVVQGIGGKYSADRIYGPAGINLMTTIAGQRAWVETTSNHFE
                     VYFVREPAGGSLEVQIDGANELQEPLSLSARTTKLDYLTFDLPDSGSHRLELRTLNNG
                     TVRLLGIVAERISPGVRYDVFGINGARANRLLSWNGPAFNAAIKALAPDLIILGFGTN
                     ELTDGDWTSETNQSLYGEILQRLRAAAPKASILIVGPPDRADVDVGVRLRLMNESLRE
                     VATLNRAAFWSTYDAMGGAGSMNKWLRQGLAQADRVHFTSAGYDALGARFYRDMMKGM
                     SYFQNPAVGTGQSFENYHLIFENFYFDR"
BASE COUNT          763 a          758 c          795 g          766 t
ORIGIN      
        1 aatagacgca gcataatcat attgattaga ggtacgttgt gtaccagctt cacttttgga
       61 tcgacgtcgc gttcagtcgc ggctttgcgc ggctttggcc tgggcgagcg acaatcagga
      121 tcgattggga agaaatatca agcagctgaa tggagggctc actcagatct tcgcttgcaa
      181 ctttaatagc cgttctgccg cgcggttcag cgtctcgtct ttcttgcaaa aacagaaacg
      241 aacttgctgc tgacccctat tcgagccgct atagaaactt gatcccggca cacaagcgac
      301 gccaatttct cgaatcaagt gcttggtaaa ctcgatgtcg tttggaaatc caaatgacga
      361 tatgtccgtc attacgtaat aggcgccgtc gggaacgaat gctcgaaatc cagctgactc
      421 cagcaccggt aacaatcgat cacgtcgcgc tcgatactcg tcttgcaacg catcgtagta
      481 ttcgccagga agcgaaagcg cataggctcc agcggcttgc aacggtgcgg cggctcccac
      541 cgttaggaag tcgtggacct tccgaatgcc gctggtaatt tccggtggtg cgatgcaata
      601 cccaactcgc caaccagtaa ccgagtaggt tttggacatc gagttaacta ccactgttcg
      661 ctctctcatg ccctcgagtt gggccatcga gatgtgagct atctcggcgc cttctctcgg
      721 gtacaggatg tgttcgtaga tctcatcagt aaaacagaga gcgtcaaact cacgacaaag
      781 accggcaatg tactcaagtt cgtcgcgcgt gaaaacttta cccgtcggat tgttgggatt
      841 gcagataatg atcgctttag tgttggagtt gaatgccgac cgcaattcgt cgcgatcaaa
      901 cgaccagtct ggttcgcgca atggcacgta tcgtggacga gcatccgaaa ggatcgcatc
      961 gggagcgtag ttttcgtagt aaggttcgaa aacgataact tcctcaccgg gatcaacggt
     1021 cgccatcatc gcggcgatca ttccttccgt cgatccgcag gtcacggtaa tttcggtctc
     1081 aggatctatt tccaaaccaa gataccaact ggtttttcga gcgatggcgt ccctgaaatc
     1141 cttcgaaccc cacgtgattg catactgatt gacatcgttt gcgatcgcct ccattgctct
     1201 acgcttgatg tcttccggag cggcaaagtc ggggaaaccc tgtgagaggt taaccgcacc
     1261 atacttcagt gcctcgcgag tcatctctcg gatcaccgat tcagtaaatg atttcgcttt
     1321 ctttgagacg cggcgatttt cggttgcggg cgttgtcatc tgcagctgtc catagccttt
     1381 agaaatctat aaacgaaagg aaaacgtatg ctctcacagg ggtattactg tggcaagcac
     1441 ggttgaccaa aaccttgtcg gtctgcgatt tatgggtgcg atgaagtcaa aaatttgctc
     1501 aactctctgc ctccattttc tgcttgcgat ctctttatct ccttcaatca gtaacgctca
     1561 ggtaaacagg ggaaagatgt ctggtccagg cctcgaaaac gcaaaagctc tggcccattt
     1621 ttacgagtcg ttggagcaag taaaggccgg cagtcgactc gagccagttc gtgttatgca
     1681 ttttggagat tcacacatcg ctgctgatgt cctgacgaga tcaattcggg aacgcttgca
     1741 gtcggacttt ggtgatggag ggccgggttt tattgtgcct ggaaatccta tggcgacgaa
     1801 acgggccggg gtaatttccg gcgcgaccga gggctgggtg gtgcaaggta ttggtggaaa
     1861 gtactcggcg gatcgcatct atggtcccgc gggcattaat ttgatgacga ccatcgccgg
     1921 tcaacgggcg tgggttgaaa caaccagcaa tcacttcgaa gtctattttg ttcgagagcc
     1981 agcaggggga tcgcttgaag ttcaaattga cggcgccaac gaactacaag aaccactttc
     2041 attatccgcg cgaacgacga agctcgacta tttgactttt gacctgcccg attcggggtc
     2101 gcatcgtctg gaacttcgca cactcaacaa cggaacagta agactgctgg gaattgttgc
     2161 ggagcgaatt tcaccgggtg tccgatacga tgtgtttggc atcaacgggg cgagggcaaa
     2221 tcgtttgtta agctggaatg gcccagcctt caatgcagca atcaaggcgc tggctccaga
     2281 tctgattatt ctcggctttg gcacgaacga attgacagat ggcgattgga ccagtgagac
     2341 gaatcaatca ctttacggcg aaattctgca gcggttacga gctgctgcgc cgaaagcttc
     2401 aatcttgatt gttggaccac cggaccgcgc agacgtagat gttggagtac gtttaaggct
     2461 catgaacgaa tcacttcgtg aggttgcgac gttgaatagg gcagcattct ggagtacgta
     2521 cgacgcaatg ggaggggcgg gttcgatgaa caaatggttg cggcaaggac ttgctcaggc
     2581 tgatcgagtg catttcacct ccgcgggata cgacgcgctt ggcgcccgct tttatcgaga
     2641 catgatgaag ggaatgagtt acttccagaa tcctgcagtt gggacggggc aatcatttga
     2701 gaattatcat ttgatatttg agaatttcta tttcgatcgg tagagactcc gactaactaa
     2761 tacccacgta aaccaattag caattctcaa atatcaaatg ataattctca aatgattgcc
     2821 ccgtcctaac tgcatgactc ttgacccctg cccttctgac cgctgaccct tttccacctc
     2881 gattggacgc cctgtgagtt aggcccgtat aatttccatc acggaaatta aggagaaaca
     2941 tcgattaaat gccgatttac gaatacgaat gtcagaaatg caaaaccaaa agtgaagtct
     3001 ttcagaaggt taacgataaa cccccagcaa agtgcctaaa atgcggcggt aaactagaac
     3061 gacttatttc cgccccggca at
//
LOCUS       DASSFP010000116         4807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_175513, whole genome shotgun sequence.
ACCESSION   DASSFP010000116 DASSFP010000000
VERSION     DASSFP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175513"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VFH91_02455"
     CDS             complement(<1..1195)
                     /locus_tag="VFH91_02455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6861871.1"
                     /translation="MIGKTLRNYHITEKLGAGGQGTVYRATDSKLGRSVVIKVLPPEL
                     TAKEANLKRFEREARLASALDHPNICTIFDLDEVDGVHFIAMQHVEGKNVRQLVAGRP
                     LELKTAVLIGLQTAEALAAAHSRGIIHRDIKSGNVMVTPSGQVKVLDFGLAKLLDDEQ
                     AATTGIHHTELTEVGVPYGTATYAAPEQARGDRVDKRADIFSLGVLLYEMLTGTWPFR
                     GKTTIDVRHSVLYDAAPPIEEMRNSPIPARLQEVVDRALAKDPKDRFQNMDNMRDELR
                     LVLQEIDSGATHLQSVAPEPPRHLGGANPVSRAMRWLKSITRSEMPTTSPNVTGPTRQ
                     GLDETPLTTVADQDKKSLAILPFRNLANDPSKSFYEFSLADAAITELARVRSLVVRPS
                     SVIAKY"
     gene            complement(1325..2305)
                     /gene="rfaE1"
                     /locus_tag="VFH91_02460"
     CDS             complement(1325..2305)
                     /gene="rfaE1"
                     /locus_tag="VFH91_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET6861872.1"
                     /translation="MNRKRAEEILTDLKRRNITVLGDVMLDEFVWGDVTRISPEAPVP
                     VVDVRKESRHLGGAANVLANLAALGASGSVVGVIGEDAAGEYLRSDLATLGPGALENL
                     IKDKSRPSTTKTRIVAHNQLVVRADRESRRLVAPEIETRIIERLKAALNEADAFVVSD
                     YDKGVVTPRILNEVLPLAYGRIPVLIDPKLRNFDSYRPATLITPNHHEALKMSNTEED
                     SDHGLKIAAEIIQERLGCDSVLITRGDRGMMLLGKDGSPVYVKTAAREVYDVTGAGDT
                     VIAALAGALATGASMFEAATFANHAAGIVVGKVGTATANATELAETFENA"
     gene            complement(2342..3499)
                     /locus_tag="VFH91_02465"
     CDS             complement(2342..3499)
                     /locus_tag="VFH91_02465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6861873.1"
                     /translation="MNSLRHIVEESPVLRAIETTASQSALGENLAQPLGPARWDWNDV
                     QRILLIRLRSIGDTVLATPSAAALKRFVPNAEIDLLVEDWVAPLLDEHPHLDRVLKVP
                     RRGISSRARIARELRTRRYDVVYNLHGGTTATFLTRATGARHRVGYKTYQYAQLHNHQ
                     APSPLELWCQQQTHSVEQQLALLGWTGVPVTDRPRTLLGISNDTSAGIQQRLDKARIS
                     GPIALIHPAAAFATKQWKTENFAEIVDYLTGKGFSCVAVAAPSEGQILQHLAANTKST
                     FAAFDDLRLPEITALASRSKLFVGNDSGIAHIAAAVGTPAVVIFGSSNIAHWRPWNRA
                     PAEIVYEQLPCQPCHGYFCEKFPAPECILRVPVDRVAGAIDRVLNSSQSQP"
     gene            complement(3503..3673)
                     /locus_tag="VFH91_02470"
     CDS             complement(3503..3673)
                     /locus_tag="VFH91_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET6861874.1"
                     /translation="MTVSQELLEILACPACKAKVELKPDGNALMCVECKRVYPIRDDI
                     PVMLIDEATIEE"
     gene            3807..4568
                     /locus_tag="VFH91_02475"
     CDS             3807..4568
                     /locus_tag="VFH91_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861875.1"
                     /translation="MSIEFALQYACPLRATYSEKHLREWGRLSSLHARLTTGDEAVPS
                     SDKINWIEHARDEVELLQEATKICAQCPASLPLENAGDGESVGCLGRINYPIEAEFEK
                     FLADRIQLSLDTLDKEDQPRLLRILIDPESPFDGEASKELRRVVTPEGLRFFELRVPI
                     GLTREGARITTDHVFDLLAGFRSEDEEVTSYTREFPLAATGDYYDFLDLLLRNELSQS
                     EVARLTARSRNYGQYLRLLSAIEKAEAFNSRVLID"
BASE COUNT         1131 a         1235 c         1248 g         1193 t
ORIGIN      
        1 gatacttagc gattactgag gaaggacgca ccaccagcga gcgtactcgt gccaattccg
       61 tgatggcggc atctgccaat gagaactcat aaaaggattt cgaaggatcg tttgcgagat
      121 tcctgaatgg caaaatggcc agacttttct tgtcctgatc ggcgactgtg gttagagggg
      181 tttcatccaa cccctggcgc gttggtccag ttacattggg cgaggtggtc ggcatttcgc
      241 tgcgggtgat tgatttcaac caacgcatgg cacgggaaac aggatttgcc ccacccagat
      301 gtcggggcgg ctccggcgca acgctctgca aatgagtagc ccccgaatcg atttcctgta
      361 ggaccagcct taactcgtcc ctcatgttgt ccatgttttg aaatcgatct tttggatctt
      421 ttgcgagggc gcgatctacc acttcctgca aacgagccgg aatcgggctg ttgcgcatct
      481 cttcaatcgg aggtgccgcg tcataaagaa cggaatgacg gacatctatc gtcgtttttc
      541 ctcggaaggg ccaggttccc gtcagcatct cgtacaggag tacccccagg gaaaaaatgt
      601 cggcccgttt gtcgacccgg tccccacggg cttgttccgg tgccgcgtat gtcgcagttc
      661 cgtaaggtac gcccacctca gttaattcgg tgtgatgaat tccagttgtt gcagcttgct
      721 catcatctaa aagtttggcc aggccaaaat caagtacctt aacttgccct gacggagtga
      781 ccatcacatt tcccgacttt atatcccgat gaataattcc acggctatga gcggccgcca
      841 aagcttcagc tgtctgtagg ccgattaaca cggcagtctt caattccaac ggacggccgg
      901 ccacgagctg tcgcacattc tttccttcaa catgttgcat agcgatgaag tgcacgccgt
      961 caacttcgtc gagatcgaag atagtacaga tattcgggtg gtccaatgcc gaggcgaggc
     1021 gcgcctcacg ctcaaaacgc tttaggtttg cttctttcgc ggtgagttcc ggaggtagaa
     1081 ccttgattac aactgatcgg cccagcttgg agtcggtcgc tcgatacacg gtgccctgtc
     1141 cacccgcccc gagtttctcc gtgatgtggt agttgcggag cgttttgcca atcatgttag
     1201 tcagcttgtc gggtggcgtc gctctgcggc gcccaattgc agtgaggtca gttaagaggg
     1261 cctacgaatc tcgtgagaat gagtaggccc aatgaatcca tgtttgggtc cgtgaaacta
     1321 cggatcatgc gttttcaaaa gtctcagcca attcggtagc gtttgcggtt gcggttccaa
     1381 cttttcctac cacgattcca gcggcatggt tggcgaatgt ggcagcctcg aacatggagg
     1441 ctcctgttgc gagcgctcct gccagtgctg ctattaccgt gtctcccgct ccggtcacat
     1501 catatacctc tctggcggcg gttttaacat agacggggct cccatctttg ccgagcagca
     1561 tcattccgcg atctcccctg gttattaaga cagaatcgca gcctaaacgt tcttgaataa
     1621 tttcagcggc gatttttaga ccatggtctg agtcttcttc ggtattactc atcttcagag
     1681 cttcatgatg gtttggggtg atcaaggtag ccggccgata agaatcgaag tttctcaatt
     1741 ttggatcaat gagaactgga atccttccgt aagccagagg taaaacttca ttgagaattc
     1801 gtggcgtgac gactcccttg tcatagtctg acactacaaa agcgtcagct tcgtttaaag
     1861 ctgctttcaa tctctcaata atcctggtct caatctccgg cgcaactaat ctacgcgact
     1921 cacgatcagc acgcacgacc agttggttgt gggcaacaat tcgagtcttc gtagtcgatg
     1981 gacgtgattt atccttaatc aagttctcaa gtgcacccgg gcccagtgtg gcaagatcgg
     2041 aacgtaaata ttcgccggca gcatcttcac cgatcacgcc cacaaccgaa cctgatgcgc
     2101 cgagagcagc aagattggcc aggacatttg ctgctccacc gagatgacgg gactcttttc
     2161 tgacatcaac aacaggcact ggagcttccg gagagattcg agtgacatct ccccaaacga
     2221 actcgtccag catcacgtca cccagcacgg tgatattacg ccgttttaga tcagtcagta
     2281 tctcttcagc gcgttttcta ttcatgtgac cgaattagtt ggaccatcaa attgtatgcg
     2341 gtcagggctg tgattgagaa ctatttagca cccgatcaat agcgccggcc actctatcga
     2401 caggtacgcg aagaatacat tctggcgcag gaaatttttc gcagaagtag ccgtggcaag
     2461 gctggcacgg aagctgttcg tacacaattt ctgcaggtgc tcgattccag ggtcgccaat
     2521 gcgcaatgtt cgatgaccca aagattacca ctgcaggagt gccgaccgct gctgcgatat
     2581 gagcgatacc gctgtcgttt ccgacgaaca gcttagagcg cgaggccagg gcagtaatct
     2641 cgggcaggcg aagatcgtcg aatgctgcga aggttgattt agtgttggca gcgagatgct
     2701 gcaggatttg tccctcgctc ggagcagcca ccgccacaca gctgaacccc ttcccggtca
     2761 gataatccac gatctcggca aaattttcgg ttttccactg tttggtagcg aaagcagcgg
     2821 cgggatgtat taaagcaatt ggaccagaga tgcgagcctt atcgagtcgt tgttgaatac
     2881 ccgcggatgt gtcgttggaa ataccaagca gcgtgcgagg tcggtcagtt actggaaccc
     2941 cggtccagcc tagcaatgca agttgttgtt cgaccgagtg tgtctgttgc tggcaccaca
     3001 actctaaggg cgacggtgcc tggtgattat gcagctgagc atattgatat gttttgtatc
     3061 cgaccctgtg tcgggcgccg gttgctcggg taagaaacgt cgcggtggtc ccaccgtgca
     3121 agttataaac gacatcatat cgtctggtcc tgagttcgcg agcgattctg gcgcgtgacg
     3181 atattccgcg tcgcgggact ttgagaacgc gatcgaggtg tggatgttca tcgagaagcg
     3241 gtgcgaccca atcttcaacc agaagatcaa tctcggcgtt tggaacaaat cttttgagag
     3301 ctgctgccga tggggtcgca agcaccgtat caccaatcga gcgtaatctg atcagcagaa
     3361 ttcgttggac gtcattccag tcccagcgag ccggaccaag cggctgcgca agattttctc
     3421 cgagggccga ctgactggcg gtggtttcaa tggcccgcaa cacaggtgat tcctcaacaa
     3481 tgtgacgcag gctgttcatt cgctattcct cgatcgtcgc ctcatcgatc aacattacgg
     3541 ggatatcgtc acggatcgga taaacgcgtt tacattcgac gcacattaag gcgttaccat
     3601 cgggtttgag ctcgactttt gctttgcaag caggacaggc gagtatctct agaagctcct
     3661 ggcttactgt catgcagatg acctttcgta gtagtgacgt tcaccgaagc ttaacatatg
     3721 ggcgcggggc tttgaacttc ccacacagca cagccgtacc gcccagtaga ctctttcagc
     3781 agaaataact aagctcgtat caagttatgt caattgaatt tgccctacaa tatgcgtgtc
     3841 ctttgcgtgc aacctattct gaaaaacact tgagggagtg gggacgttta agcagtcttc
     3901 atgcacgtct cacaacgggt gacgaagccg taccttccag cgacaagatc aattggatcg
     3961 agcatgcccg ggatgaagta gaactgctac aggaagcaac aaagatctgt gcacagtgtc
     4021 ccgcaagttt accgctcgag aacgccggtg atggtgaatc agtcggctgt cttggacgaa
     4081 tcaattaccc aatcgaggct gagtttgaaa agtttctagc tgataggatt cagttgtcgc
     4141 tggatacgct cgataaagaa gaccagccac ggttgcttcg tatcttaatt gaccctgaat
     4201 cgccatttga cggcgaagcc tccaaagagt tgaggcgagt tgtcacacct gaagggttgc
     4261 gattcttcga gttaagagtt ccgatcggac taacacggga aggtgcgcgg attacgacag
     4321 accatgtctt cgatctgctt gccggctttc gctcagaaga tgaggaggta acaagttaca
     4381 cccgtgagtt cccgctggcc gccaccggag attactatga cttcctggac ttgttattgc
     4441 gaaacgagct cagccagtca gaggtagcga ggttaaccgc acgcagtcgg aactacggcc
     4501 aatatctgcg cttgttgtca gcgatcgaaa aagccgaagc attcaacagt cgagtactca
     4561 ttgattgaaa aaactctatt ggtcatcttg ttcctttcaa gtcacaccga cgcaaatgag
     4621 aaatgatcaa tgaggaatga tcaatgacca ataatcctga gccactaccc tctaacctac
     4681 tcatatgcca aagtacgtcg cctttctacg agccattaac gtgggtggtc atattgttaa
     4741 gatggaccaa cttcgcaaac tttttgaggc cctgccgttt gataatgtcg aaacattcat
     4801 cgcgagc
//
LOCUS       DASSFP010000117         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1962349, whole genome shotgun sequence.
ACCESSION   DASSFP010000117 DASSFP010000000
VERSION     DASSFP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962349"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VFH91_02480"
     CDS             complement(<1..1211)
                     /locus_tag="VFH91_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6861876.1"
                     /translation="MTNKYVGKPIKRTEDPRLIKGLAHYVDDIRLPDTLHVAFVRSVY
                     AHAKIKSVDISAALESPGVVAVYLGKDVQKVGPVPCASALPELKVPDHRVLAGDKVYF
                     VGHPIAAIVATDRYAARDAVDLVMVDYEDLPVVVDVEQAAKGGPVIHENFADNIAYKL
                     TSGEGDIEAAISSADHVVKQRILHNRLAPIAMEPRGVLARYLPGEEELTVWSSTQIPH
                     LLRTQLAIMIGIPENKLRVITPEVGGGFGSKLNVYGEEALIGWISVQLGKPVKWIETR
                     RENMQATIHGRGQVGDIEIAFKNDGTLTGLRYSVFADLGAYHQLLTPAIPTLTGLMLS
                     GAYKIPAIQMNITGCFTNKMATDAYRGAGRPEATYVVERALDLVAAELKMDPAELRRK
                     NFPAASEFPFHT"
     gene            complement(1232..1711)
                     /locus_tag="VFH91_02485"
     CDS             complement(1232..1711)
                     /locus_tag="VFH91_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6861877.1"
                     /translation="MKKAINLTVNGVSRSDEVEPRMLLVHYLRDVLALTGTHVGCETS
                     LCGACTVFIDGKAVKSCTMFAVQTEGASVTTIEGLAKNGELHPVQEGFWEKHGLQCGY
                     CTPGMIIAAAQLLDRNQSPSRKEICDGLEGNLCRCTGYEHIIEAVEYAAAKGAGSSK"
     gene            complement(1825..2145)
                     /locus_tag="VFH91_02490"
     CDS             complement(1825..2145)
                     /locus_tag="VFH91_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861878.1"
                     /translation="MDLTFEQLRHKTLADLREIAKGVDHEAVQGYTQLNKERLVIALS
                     KALGIQHEHHDVVGVDKAAIKARIRELKAKRNEVIDAHDRYALKTIRRTIHRLKRQIH
                     KATV"
     gene            complement(2156..2671)
                     /locus_tag="VFH91_02495"
     CDS             complement(2156..2671)
                     /locus_tag="VFH91_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861879.1"
                     /translation="METDRQNIESFLQDFRETIHSATTRLRELEARTNSSSHPAQEWT
                     AKEILGHLIDSAANNHQRFVRAQLMDDLVFPGYEQNSSVELQNYNDESWLDLIQLWSL
                     YNLHIVHVITQIPDKVLTAPRTVHNLDQVAWKPVDKSRPATLLYFVRDYLGHLKHHLN
                     QIYDRTDKSGC"
BASE COUNT          620 a          693 c          703 g          720 t
ORIGIN      
        1 gtgtggaaag ggaattcgct agcggcgggg aagttcttcc tgcggagttc ggccgggtcc
       61 atcttcaatt ccgccgctac caaatcgagt gctcgctcaa ctacataagt tgcttcgggt
      121 cgtccagcac cgcgataggc atcagttgcc atcttgtttg taaaacaccc ggtgatgttc
      181 atctgaatgg ctggaatttt gtaagcgccg gaaagcatca atccagtcaa cgtcggaatt
      241 gccggagtaa gcagttgatg ataggcgccg aggtcagcaa agacgctgta acgtagaccg
      301 gtgagagttc cgtcgttctt gaaagcaatc tcaatgtctc ccacttgccc gcgaccgtga
      361 atcgtagcct gcatattttc acgtcgcgtc tcgatccact taaccggttt acctaattga
      421 accgaaatcc agccgatcag tgcctcttca ccatagacgt tgagcttaga tccaaaaccg
      481 ccaccgactt ctggcgtaat gacccgcagt ttgttttcag gaattccgat catgatcgcc
      541 agctgtgtgc gaagcagatg agggatctgg gtagatgacc agaccgttaa ctcttcttct
      601 cctggcaggt atcgagccag aacaccacgc ggctccatgg caattggagc aagccggttg
      661 tgtaggatgc gttgtttgac gacgtgatcc gctgaagaga ttgcagcctc gatgtcacct
      721 tcgcctgaag tcaatttgta agcaatgttg tcagcgaagt tctcatgaat gactggtcca
      781 cccttcgctg cctgttcgac gtctacgacc acaggcagat cttcataatc aaccattacc
      841 agatccacgg cgtctcgggc agcatagcga tccgttgcga caattgctgc gattggatgg
      901 cccacaaagt agaccttgtc accggctaac acgcgatgat caggaacctt taattccggc
      961 agcgcacttg cacagggaac gggcccaacc ttctgaacat cttttcccag ataaaccgcc
     1021 actacgccgg gcgactcgag agcggcgctg atatctacgc ttttaatttt tgcatgagcg
     1081 tagacggatc gaacgaaggc aacgtgaagt gtgtctggaa gcctaatgtc gtcgacatag
     1141 tgggccaggc ctttgatcag tcgaggatct tcggttcgtt ttataggctt gcccacgtac
     1201 ttgttggtca tgtgaactcc ttcgtaagcc gttattttga cgagccggca cctttagctg
     1261 cggcgtattc aactgcttca attatgtgtt cgtagccggt gcagcgacag agattgcctt
     1321 cgagcccgtc gcagatctcc ttgcgtgatg gcgactggtt tcgatccaat agctgcgcag
     1381 cagcgatgat catcccggga gtgcagtacc cacactgtag accatgcttt tcccagaatc
     1441 cttcctggac cggatggagc tcgccgtttt ttgccaggcc ctcaatagtg gtaactgatg
     1501 caccctcggt ctgaacggca aacatggtgc acgatttcac cgctttaccg tcgatgaata
     1561 ctgtgcatgc accgcacaaa gaggtctcac atcccacatg cgttccggtt aatgcgagaa
     1621 cgtcgcgcag gtaatggacc agaagcattc tgggttccac ctcatcagag cgagaaacac
     1681 cgttgacggt taggttgata gccttcttca tgagctacct cgcttttctt ttgatggctg
     1741 ttaagatttt gttactgagc gctttctaag tcagtttgca ctgaaagtca atgcttagtc
     1801 acctaagcgt aaacctccag cctgctaaac cgtagcttta tgaatctgac gtttcagccg
     1861 gtggatggtt cggcgaatcg tttttagggc gtaacgatcg tgcgcatcga tcacttcgtt
     1921 acgcttggct ttgagttcgc gaatccgagc tttgatggcc gccttatcca cgccgacgac
     1981 gtcgtgatgc tcgtgttgga ttcctagtgc cttggacaac gcaatcacga gccgctcttt
     2041 gttcagctgg gtgtagccct ggactgcttc gtggtccacg cccttggcaa tttcacgaag
     2101 atccgcaaga gttttatgtc gaagttgttc gaaagttaga tccatacgat cctcctcaac
     2161 agccggactt gtctgttcgg tcgtagattt gattcaggtg atgtttgaga tggcccagat
     2221 aatctctcac gaaataaaga agcgtggcgg gccgactttt atcgacaggc ttccaggcga
     2281 cttgatcgag gttatgaact gtccgcggcg cggttaaaac tttatcggga atttgtgtga
     2341 tgacgtggac gatatgcaaa ttgtagagag accacagttg aataaggtcc aaccacgact
     2401 cgtcgttgta gttctgcaat tcaaccgagg agttctgttc gtaaccagga aagaccagat
     2461 cgtccatcag ttgagctctg acgaatctct gatgattgtt tgctgcggaa tcaatcagat
     2521 gtcccaggat ctcttttgcg gtccattcct gagccgggtg tgacgaagaa tttgttctcg
     2581 cctctagctc gcgaagacgg gttgtcgctg aatgtatagt ttcgcgaaag tcctgtagaa
     2641 aactctcgat gttctggcga tcggtctcca tcgaaaaatg tctctgaatt agcggtgctc
     2701 agatgatagc gcaatgactt gctgctagaa aagtct
//
LOCUS       DASSFP010000118         5861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_473335, whole genome shotgun sequence.
ACCESSION   DASSFP010000118 DASSFP010000000
VERSION     DASSFP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473335"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFH91_02500"
     CDS             complement(<1..493)
                     /locus_tag="VFH91_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET6861880.1"
                     /translation="MRRIVSKIASNSLQREAPYSVLLPSAYDSSDLGYPILYLLHGLF
                     GSYENWVELTNIEELSSNLHLIIVMPDGSDGWYCDSETVSANRYESFLIEELIPEIER
                     KFRTIPKREARAIAGLSMGGYGAFKFSVRRPDLFQFAASFSGAFDVPERSDDAPGFDW
                     NTLR"
     gene            630..2396
                     /locus_tag="VFH91_02505"
     CDS             630..2396
                     /locus_tag="VFH91_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HET6861881.1"
                     /translation="MSPGHSNYDLQPHTQIASSVQCHQGWEEILGAIHRNVTSGQFIL
                     CIECYPGVPVSEISDRVKAVVEPALVIESEKLFRPELELNEMLQPYLGDDPVFGRLNP
                     IEIEDFFDTKNLSDAREQVSRAGNEIVLLVGPGASLLSNKIDLLVLAEISRWNIQQLQ
                     RQKVIGNLGTNNLEDSPGNKYKRAFFVDWRSADRLKVKIFSEIDYLLDLNNPHAPRMI
                     SGDVYRRGLDTVANRPFRVVPFFDPGPWGGQWMKRAFNLPPDKLNYAWCFDCVPEENS
                     LLLGFGDRVVEVPALDLILTHPKEVLGPAIFEEFGAEFPIRFDFLDTVEGGNLSLQVH
                     PLRSYISEHFNIPYTQDESYYILDQSGESVVYLGIKDGVEPAAMAAALQKAQAGDAPF
                     AAEEFVNTWTSHKHDHFSVPGGTVHCSGRNNVVLEISATPYIFTFKLWDWGRMGLDGR
                     PRPIHLDHGLANIQWNRTTEWVREQLFNKNEFVAEGDGWREERTGVHHSNFLETRRHW
                     FTGTVPHDTKGTLNVLNLVEGEEVVVESPQDRFPPFVVHYAETFIVPAAVGPYTIRPA
                     RQPEKPYATIKAFVRNASIDGN"
     gene            2400..3347
                     /locus_tag="VFH91_02510"
     CDS             2400..3347
                     /locus_tag="VFH91_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6861882.1"
                     /translation="MYILGIDIGGTRLKLARVDHDGTILDRKIKETPTTLEDFSDTLT
                     LLIKQLIDGSEGPVAVGVGCKGVIDTHSSTVLRQPGTFSFLEGLNFPRLLNPLFKRPV
                     PVHADNDARVALAGEVIWGAAKGKRNVVMLTLGTGVGGALLVDGRIVRGEKGGAGLLG
                     HLTVAPDGRFCDCGNRGCLETVFSARAIEAEALHAVYRGTESLLTERFKDDLSQLTCE
                     SVFQAAADGDNVASVIRDEATSTLAAAIAGLLHVFDPEVVILGGQIVEAGDSLLEPIR
                     DEVAWRTKRFLGRVVPIVGSEVQDSSGVVGAAALTLIDG"
     gene            3445..4740
                     /locus_tag="VFH91_02515"
     CDS             3445..4740
                     /locus_tag="VFH91_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET6861883.1"
                     /translation="MKQPRAQHLKMIGVREVRDINQTMFLHLIRERQPISRADIAKAT
                     GLRPGTVSAIVNRLIREKLVYEGVEGPSTGGRPPKYLYVNAESAYVLAIDVGVSETLY
                     AVSDFNGRIMMQRAVVTEGSPETFLVSLARDIKTLIQSQYPRARFEAVGVSVPGLVDR
                     ADGSLIISPNLGWQSVPVRALLESELMLPVFVENDANASAFAELWYGPLDELSTRTLL
                     FVLVVEGLGTGLIINGELHVGTSIGLGGFGHMTLDPNGPVCSCGRRGCWETFASDRAT
                     VDRYNERKDVQSRLRTVTQIIALAKQGDSAALEVIETTARYLGEGIVSLAHGLSPQVI
                     VLGGEIAGAWPIVEPIVRECVKSNYIIPPINIVIRPSSVQRPGLFGAIPIALQHLFGD
                     RSDLHDSNGKKSTSANDWSRRLVGPGTARYALGSQLTKK"
     gene            4996..5072
                     /locus_tag="VFH91_02520"
     tRNA            4996..5072
                     /locus_tag="VFH91_02520"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5030..5032,aa:Ala,seq:cgc)
     gene            complement(5168..5362)
                     /locus_tag="VFH91_02525"
     CDS             complement(5168..5362)
                     /locus_tag="VFH91_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861884.1"
                     /translation="MSIDFQEGGTTEVTQPTRDAALLQAEEIARSQMARQRKKLGTLT
                     CEQEIGIEMLLRSTALKISE"
     gene            complement(5384..5590)
                     /locus_tag="VFH91_02530"
     CDS             complement(5384..5590)
                     /locus_tag="VFH91_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861885.1"
                     /translation="MSSIIKVVKRNERKVLEEKARNATKEIKQTTEMIIKNWIEASRE
                     KRRVEAESSFQDFKRSQLRPLLES"
BASE COUNT         1427 a         1452 c         1506 g         1476 t
ORIGIN      
        1 gtctcagcgt attccagtca aagccaggtg catcatccga gcgctctggt acgtcaaaag
       61 ctccactgaa agaagcagca aactggaata gatcgggacg gcgaacggaa aatttgaaag
      121 ctccataccc tcccatcgag aggcccgcta ttgctcgagc ctctctctta ggaatagttc
      181 gaaattttct ttcgatctcg ggtattaatt cttcaatcaa aaaactttcg tatcgatttg
      241 cagaaaccgt ttcgctatcg cagtaccaac catcgcttcc atccggcatg acaattatta
      301 gatgcagatt gctcgaaagt tcttcgatat tggttagctc gacccaattc tcgtagcttc
      361 caaacaaccc gtggagcaga tacaagattg gatagccgag atccgaggaa tcgtacgcag
      421 acggaagaag caccgaatat ggcgcctcgc gctgaaggct gttactggcg attttactaa
      481 caattctccg cattctcgag attggcccgt ggtggccact gcctgtaata ctctttatct
      541 tgaccggtga caaagtagcg agcgttgtgt aacatacgaa acttcacggt gtcaaaaacc
      601 gagcagttca ggaaagcaga agttaatccg tgtcaccagg tcattcgaat tacgatcttc
      661 aaccacacac tcagatagct tcaagtgtgc aatgtcacca gggctgggaa gaaattctcg
      721 gcgccattca tcggaatgtc accagtggtc agttcattct ctgtatcgag tgttatcccg
      781 gcgtgccggt ttctgagata agtgaccgcg tgaaggcggt ggttgaacca gcgctcgtca
      841 tagaaagcga aaaactgttc cgcccggaac tcgaacttaa tgaaatgctc cagccgtacc
      901 tgggagatga tccggtgttt gggcgattaa accctatcga gatcgaggac ttctttgaca
      961 caaagaatct gtccgacgcc cgcgagcaag taagccgcgc cggaaacgag atcgtactgc
     1021 tggttggacc aggcgcttcc cttctctcaa acaagattga cctgctcgtt cttgctgaga
     1081 taagccgttg gaacattcag cagctccaac ggcagaaagt aatcggtaat ctggggacaa
     1141 acaatttaga agactcgcct ggaaataaat acaaaagagc tttctttgtt gattggcgca
     1201 gtgcagatcg gttgaaggta aagatctttt cagagatcga ctacctgctt gacctcaata
     1261 acccgcacgc cccccgaatg atctccggtg atgtgtacag acgtgggtta gataccgttg
     1321 cgaaccggcc atttcgcgtg gttccgtttt tcgatcctgg accatggggc ggccaatgga
     1381 tgaagcgcgc gtttaacttg ccgcccgaca agctcaacta cgcctggtgt ttcgactgtg
     1441 tgcctgaaga aaacagctta ttgctgggct tcggcgaccg ggtagttgag gttccggctc
     1501 tcgatctaat tctgacccat ccgaaggaag tgttgggacc ggccatcttc gaagagtttg
     1561 gtgcagagtt tcccatcagg ttcgattttc tcgatacggt ggaaggcgga aatctttctc
     1621 tgcaggttca cccgctccga agttatattt cggaacactt caacatcccg tacactcagg
     1681 atgagagcta ctacattctg gaccagagtg gtgaatccgt ggtctacctt ggcatcaagg
     1741 atggagttga acctgctgca atggctgcgg cgttacaaaa agcccaggcg ggtgacgctc
     1801 cgtttgcggc ggaagagttt gtcaacacgt ggaccagtca taagcacgac cacttttcag
     1861 ttccgggggg aacggttcac tgttcgggtc gcaacaatgt agtcctcgag atcagcgcga
     1921 ccccttacat attcacgttc aagctttggg attggggaag aatgggactc gatggccggc
     1981 cacgtccgat tcacctggat catggattag caaacatcca atggaatcgc accacagagt
     2041 gggttcgtga acaactcttc aacaagaatg agtttgtcgc tgagggtgac ggctggcgcg
     2101 aagaacgaac aggcgttcat cattcaaact ttcttgagac acgccggcac tggttcaccg
     2161 gcacagtacc gcacgacacg aaaggcactt tgaacgtact caatctcgtg gaaggtgaag
     2221 aggttgttgt ggagagcccg caagatcgct ttcctccctt cgtcgtacac tacgccgaga
     2281 ctttcattgt gccggcggct gttggtccat atacaatccg gccggcgcga cagccggaaa
     2341 aaccatacgc aaccataaaa gcttttgtta gaaacgcgtc catcgacggc aattaactca
     2401 tgtacatttt aggcatcgac atcggaggta ctcgtctcaa gctggccagg gtcgaccatg
     2461 acggcaccat actcgatcgg aaaattaagg agaccccaac aacactcgag gatttttcag
     2521 acactttaac tttgcttata aaacagttga ttgatggaag cgaaggcccg gttgctgttg
     2581 gcgtaggctg taaaggcgtg atcgacaccc attcgtcaac tgtattgcga caacctggaa
     2641 cgttcagctt tctcgaaggt ctgaattttc cacgtttgtt gaatccgttg ttcaaacggc
     2701 cagttcctgt tcatgctgac aacgacgctc gtgtcgccct ggcgggtgaa gttatttggg
     2761 gggctgcaaa aggaaaaaga aatgtcgtga tgttgactct gggaacaggt gtcggaggtg
     2821 ctttgttggt tgatggccga atagtcagag gtgaaaaagg cggcgctggt ctgctgggcc
     2881 atctcactgt tgctcctgat gggcgtttct gcgactgtgg aaatcgagga tgtctggaaa
     2941 cagttttttc ggcccgtgca atagaggctg aagcattgca cgcagtttac cgaggcaccg
     3001 agtcgctgct gacagaacga ttcaaggatg acctgagcca attaacttgc gagtcagttt
     3061 ttcaggcggc ggccgatgga gataacgtgg cttcagtcat tcgtgacgag gcgacatcca
     3121 cgctcgctgc ggctatcgct gggctactcc acgtatttga tccggaagtc gtgatactgg
     3181 gtggacaaat tgttgaagca ggcgactcgt tacttgagcc gatacgcgac gaggtagcct
     3241 ggagaacgaa acgttttttg ggacgagtag taccaatcgt tggttctgag gtacaggact
     3301 cgtcaggcgt agttggagcg gcggcactga cacttatcga cggctaaaaa acaggtaact
     3361 atggtgccct cgtgatcatg ctgtgttaaa ttcaagcaga gaccttccgg cattacggat
     3421 cccatttaca actctcagtt aagaatgaaa caaccgcgcg cgcaacatct gaagatgatc
     3481 ggagttcgag aggtacgcga catcaatcaa acgatgtttc ttcacctcat tcgcgaacgg
     3541 caaccgatct ctcgcgccga cattgccaag gcgaccggtc tcaggccggg aactgtttct
     3601 gcaatcgtca atcggcttat cagagaaaaa ttggtctacg aaggagtcga aggtccttca
     3661 actggtggac gtcctcctaa atatctttac gtcaatgcgg aaagcgcgta cgtgctggca
     3721 attgatgtag gcgtcagtga aacactctac gctgtaagtg acttcaacgg ccggatcatg
     3781 atgcagcgag cggttgtgac cgaaggctcg cctgaaacgt ttttagtttc actcgcgcgc
     3841 gacatcaaga ctttgatcca atcgcagtat ccgcgtgctc gattcgaagc agtcggcgtc
     3901 agtgttccag gcctggtcga ccgtgcggat ggttctttga tcatctctcc caatcttggc
     3961 tggcagtcgg tgcctgtgcg cgcactgctc gaatcagaat tgatgttacc ggtgtttgta
     4021 gagaacgatg ccaacgcttc tgctttcgca gaactatggt atggaccact cgacgagttg
     4081 agtactcgta ctcttctttt tgttctcgtg gttgagggac ttggcactgg tttaatcatc
     4141 aatggcgaat tgcacgtcgg taccagtatt ggtctcggcg gtttcggtca tatgactctc
     4201 gatccgaatg gaccagtttg ctcttgcgga cgccgtggtt gttgggaaac gttcgcttca
     4261 gatcgagcta ccgtcgacag gtacaacgag cgcaaagacg tccagtccag gctgcgcact
     4321 gtgacacaga taattgcgct cgcaaagcag ggtgattcgg cggcgttaga ggtaatcgag
     4381 actactgcaa gatatcttgg cgagggaatt gtgagtctgg ctcatggttt gagcccgcag
     4441 gtaattgtcc tgggaggaga gattgccggc gcctggccca tagttgagcc aattgtgagg
     4501 gagtgcgtaa agagcaatta cataattcca ccgatcaaca tcgtgattcg tccttccagt
     4561 gttcaacgtc cgggcctgtt tggtgctatt ccaatcgccc tccagcatct attcggcgat
     4621 cgctcagatc tccatgacag caacggaaag aagagcacta gcgctaacga ctggtctcgc
     4681 cgtctcgtcg gtcccggcac cgcccgctac gccctgggtt ctcaattaac taagaaataa
     4741 cttttggagt gcgcctttgg agtgcgccgg cccggcggcg ctttgttatc caatccccta
     4801 catttttcat tcgccatttt tgccccattt tattcaacca ccccgatatg cagacttgaa
     4861 aaaacgaaaa agaaaaatga gcaattagca atgagcaatg gaaaatgaca aatggaaaat
     4921 gatccgcagc acgcgcgctt tcttgaacct taacttgact gcccacttac tcgacttcta
     4981 tcatgcaacc cgcgtggggc agtagctcag tttggcagag cgccgcgttc gcaatgcgga
     5041 ggtcaggggt tcgacccccc tctgctccac caagtaacta ccttttcttc aatcacttac
     5101 aagaaacaga caaattagaa cagtgtctac tccttaaccg cctggcagtg ttccaagacg
     5161 cttccagtta ttccgagatt ttgagagcag tcgatcgtag aagcatttcg atcccgatct
     5221 cttgttcaca agttagtgtc cccagcttct ttcgctgccg agccatctga gatcgagcaa
     5281 tctcttcagc ttgtagtaaa gcagcatccc ttgttggttg cgtgacctcg gtcgttccac
     5341 cttcttgaaa atcaatactc atgatcgttc accacggggg tcactaagat tccaataaag
     5401 gccttagctg cgatcgtttg aaatcctgga aggaagactc ggcctcaact cggcgttttt
     5461 cgcgtgatgc ctcaatccaa ttcttaatga tcatttccgt tgtttgtttg atttctttcg
     5521 tagcgtttcg agctttctct tccagaactt ttctttcgtt gcgcttaacg acttttatga
     5581 tggaactcat ctcactttac ctcggctaaa aaaaatgcaa gcaatcgaat gaactgccct
     5641 gcaactggac tgccaaaggg aatttgaggg tcaaggcgta ggaatgctca gacattcagt
     5701 tacttgtcgt agggaaaatt gtcgaaagtt gaattggcaa ctgatgggcg aaaccgtcaa
     5761 ttccggcaat ctttgccgcc ggggatgtta agtacgtgtg aattctgctg attgagagtc
     5821 cttctttttg gtacatgcga tgccaaccca aaaacttacg t
//
LOCUS       DASSFP010000119         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1512915, whole genome shotgun sequence.
ACCESSION   DASSFP010000119 DASSFP010000000
VERSION     DASSFP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512915"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFH91_02535"
     CDS             <1..838
                     /locus_tag="VFH91_02535"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6861886.1"
                     /translation="RRQTQKLFRNHMKICRIKHSSFPEPRFGVIEGDDVLVLLSDFTF
                     LNSQNRRFESKLKLDDVSLVAPVTPSKIVCVGRNYREHAAELGNKMPDEPLLFLKPPS
                     SLIASRDRIELPAASQQVEHEGELGVVIGKQARKLSDQDNPLDYIFGYTCVNDVTARD
                     LQRKDVQFTRGKGFDTFCPVGPWVVTDLDAQATQVMTRVNGTVKQDGNTRDMAFPVSF
                     LIRYISAIMTLNPGDLIATGTPAGVSRLNHGDIVEIEVGGVGVLSNEVYDPRRDVEES
                     KQ"
     gene            857..1510
                     /gene="fsa"
                     /locus_tag="VFH91_02540"
     CDS             857..1510
                     /gene="fsa"
                     /locus_tag="VFH91_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET6861887.1"
                     /translation="MKFFLDTANLNEIREAAAYGFADGVTTNPTLISKEGNVDFKQHI
                     AAICEIVQGPVSAEVTSLDKDGMVREGLEYSKIAPNVVIKCPLTKEGLKATRQLSDGG
                     IKVNVTLCFSAAQAIIAAKAGASFISPFLGRLDDIGQNGLQLLQEIVEIYQNYTWKTE
                     ILAASLRHPIHVIEAARMGADIATMPFKVIDQLFSHPLTDKGQAQFLADWAKSGRKA"
     gene            1494..1988
                     /locus_tag="VFH91_02545"
     CDS             1494..1988
                     /locus_tag="VFH91_02545"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET6861888.1"
                     /translation="MEERLSKQTEFVVKVLKYDGTEHRNWRARLAAHEGELIVLDATF
                     AEEVQHDLLGQIASGTISTEYYWLNRWYNIFRFSEPNGRLLSFYCNVNLPPELTGQTL
                     TYVDLDIDILVKPNFAYQILDTEDFEANIERYGYTHEVQAGVENATHELVELIKAQSF
                     PFNV"
     gene            1993..>3464
                     /locus_tag="VFH91_02550"
     CDS             1993..>3464
                     /locus_tag="VFH91_02550"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET6861889.1"
                     /translation="MTSKQQTRIASKSKLDRLRQRQTEAEAGGGAARVEKQHEAGKLT
                     ARERIEFLLDDGTFEEFDKLVVHRSKDFGLEKQLFPGDGVVTGYGLIDGRSVFVFAQD
                     FTVFGGSLSETHAEKICKIMDLAMKVGAPIIGLNDSGGARIQEGVLSLGGYADIFLRN
                     TLASGVVPQISCIMGPCAGGAVYSPAITDFNVMVKDTSYMFITGPDVIKTVTHEEVTK
                     EALGGAVTHNTISGVAHFAADSDEHALRIVRELLSFLPANNLENPPAIESNDPIDRAE
                     SKLNEIIPEASNQPYDIRDVINLVVDDGYFFEVQQMFAPNICIGFARLGGRSVGIVAN
                     QPAFLAGVLDINASLKAARFVRFCDCFNVPLVTFEDVPGFLPGVSQEHGGIITHGAKL
                     LYAFAEATVPKLTVITRKAYGGAYCVMASKHIRTDINLAWPTAEIAVMGAEGAVGILY
                     RRELSEAKDQDQARKQKVLELEEKFANPYVAAERGFVDEV"
BASE COUNT          900 a          807 c          918 g          839 t
ORIGIN      
        1 tcggagacag actcagaagt tgttccgtaa ccacatgaag atttgtcgca taaaacattc
       61 aagttttccg gagcctcgat ttggagtgat cgagggtgat gatgtacttg tgctgctctc
      121 tgatttcacg tttctaaatt ctcaaaaccg aaggtttgag tctaagctta aactggacga
      181 tgtaagtctg gttgctcccg tgactccatc caagattgtt tgtgtcggta gaaactatcg
      241 agagcacgcg gctgagttag gcaataaaat gcctgatgag ccacttctct ttttaaaacc
      301 tccgtcgtct ttaattgcgt cccgagatcg cattgaatta cccgcagctt cacaacaagt
      361 ggagcatgaa ggagagcttg gagttgtcat tggaaagcaa gccagaaaat tatccgatca
      421 ggacaatcca ttagattaca tctttgggta cacttgcgtt aacgatgtga ccgcgcgtga
      481 cctgcagcgt aaggacgtcc agtttacgcg tggcaaaggg ttcgacactt tttgccccgt
      541 tggaccatgg gtagttaccg atttggacgc acaagctacg caggtaatga cgcgagtgaa
      601 tggcacagtc aaacaagatg gcaatacgcg cgacatggct tttcccgtgt cgtttctgat
      661 tcgctatatc tctgcaatca tgacgctaaa tccgggcgat ttaattgcta ctggtactcc
      721 ggctggagtc tcaaggttaa atcacggtga cattgtggag atcgaagtcg gaggagttgg
      781 agttctgagc aatgaggttt acgatccacg aagagacgtg gaagaatcga agcaatagtt
      841 gagttaggag ttcgttatga aattctttct ggataccgca aatctgaatg agattcgcga
      901 agcagcggca tatgggttcg ccgacggagt aacgacaaac cccactctga tttcgaaaga
      961 gggtaacgtc gactttaaac agcacatcgc ggctatttgc gaaattgtgc agggaccggt
     1021 ttcggccgag gtcactagcc tggacaaaga cgggatggtg cgagaagggc tcgaatattc
     1081 gaagattgct ccaaacgttg ttatcaaatg tcctttgact aaagaggggc tgaaagcaac
     1141 tcgacagtta tcggacggtg ggataaaggt aaacgttact ctttgctttt cggcggccca
     1201 ggctatcatt gcagcaaaag ccggcgcttc tttcattagc cctttcctgg gcaggctcga
     1261 tgacatcggc cagaatggac ttcagttact ccaggaaatt gtcgagatct atcagaacta
     1321 cacctggaag accgaaattc ttgcagccag tttgcgacac ccaattcacg tgatagaagc
     1381 agcgcgaatg ggtgctgata tcgccaccat gccattcaaa gtcatcgatc aacttttcag
     1441 tcatccgttg accgataaag ggcaagctca gtttttggcg gattgggcca agagtggaag
     1501 aaaggcttag caagcaaact gagttcgtcg taaaggttct caaatacgac ggcacagaac
     1561 atcgaaactg gcgagctcgc cttgctgcgc acgagggcga gctcattgtt ctggacgcga
     1621 catttgctga agaagttcaa cacgacctgc tcggccagat cgcgtcaggc acaattagta
     1681 ctgagtacta ctggctgaat cgttggtata acatcttcag attcagtgag ccgaatggtc
     1741 gcttgctaag tttttactgc aatgtgaacc tacctccgga attgacgggt cagactctaa
     1801 cttacgtcga tctcgacatc gacattctgg taaaacccaa cttcgcctac cagattcttg
     1861 atactgaaga ctttgaagcc aatattgagc ggtacgggta tactcacgag gtccaggccg
     1921 gggttgaaaa tgcgacccat gagttggttg agctaatcaa agcgcaatct tttccattca
     1981 acgtgtaact ttatgacttc aaaacaacaa accaggatcg cttccaagtc gaagctggat
     2041 cgtctgcgcc agcggcaaac tgaagctgaa gcgggtggcg gcgccgcacg agtagaaaaa
     2101 caacacgagg ctggaaagct tactgcgcgg gaacgaatcg agtttctcct ggacgatgga
     2161 acgttcgaag agttcgataa gctagtggtc caccgctcaa aagattttgg tcttgaaaag
     2221 caattatttc ctggcgacgg agttgtgacc ggttacggat taatcgatgg ccgatcagtg
     2281 tttgtgttcg ctcaagactt cacggttttc ggaggctcac tctctgaaac acatgcggaa
     2341 aagatctgca agattatgga tctcgccatg aaggtggggg ccccgatcat agggctcaac
     2401 gattccggag gcgcgcgcat acaggaaggt gtcctgagcc tcggaggtta cgccgatatc
     2461 ttccttagaa atacgcttgc gagcggagta gtacctcaga tcagctgcat catgggccca
     2521 tgtgccggtg gagctgttta tagtccggcg atcaccgact ttaacgtgat ggtcaaagac
     2581 acgtcataca tgttcataac tggaccagac gtgatcaaaa ccgtgacaca tgaggaagtc
     2641 acgaaagaag cgctcggtgg tgcagtcact cacaacacaa tttcaggcgt ggcccatttc
     2701 gccgccgatt ccgacgagca cgcgttacga atcgtacgcg aacttctctc gtttctgccg
     2761 gcgaataacc ttgaaaatcc tccggcaatc gagtccaacg atccgataga ccgcgcagag
     2821 tcaaaactca atgagattat cccggaggct tcgaaccagc cgtacgatat tcgagatgtc
     2881 atcaatctcg tggtcgatga cggctacttt ttcgaagtgc aacagatgtt tgcaccaaat
     2941 atctgcattg gtttcgcgcg gctgggcggc agatcagttg gaatcgtcgc caatcaaccg
     3001 gcatttctgg ccggcgtact cgatattaac gcgtcattga aagctgcgcg ctttgtccgc
     3061 ttctgcgact gtttcaatgt accgcttgtc acgtttgaag atgtgcctgg ctttttgccg
     3121 ggagtttcgc aggaacacgg gggaataatc actcacggag caaagttact ctatgcgttt
     3181 gccgaggcca cggttcccaa gctaactgtc ataacgcgaa aagcctatgg cggcgcttac
     3241 tgcgtgatgg cgtcgaaaca tattcgtacg gacataaatc tcgcgtggcc cactgctgag
     3301 atagcggtaa tgggagctga aggcgccgtc ggcattttgt atcgtcgcga gcttagcgag
     3361 gctaaggatc aggaccaggc aaggaagcaa aaggtacttg aactggaaga gaagtttgca
     3421 aatccctacg tcgcagcgga gcgaggattc gtggatgagg tgat
//
LOCUS       DASSFP010000120         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1356957, whole genome shotgun sequence.
ACCESSION   DASSFP010000120 DASSFP010000000
VERSION     DASSFP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356957"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFH91_02555"
     CDS             <1..556
                     /locus_tag="VFH91_02555"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6861890.1"
                     /translation="RPFHVLATQNPIEMEGTYPLPEAQLDRFMFMVKIGYLPEEDEVA
                     VVKQTTSNQETEFERLMSADEILAFQKLVKQVPASDTVTRYAVRLVNASRPNTPSAPD
                     FINKWVTWGGSLRASQFLILGGKARAILRGRYNVSCEDVRAVAPAVLRHRVLLNFQAE
                     AEKVDSDQVIQKLIETVPEPKSGL"
     gene            786..1661
                     /locus_tag="VFH91_02560"
     CDS             786..1661
                     /locus_tag="VFH91_02560"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6861891.1"
                     /translation="MDPELLARIGSLELLARAVVEGFMSGLHRSPFTGFSTEFTEYRQ
                     YNPGDDLRYLDWRLLGRTDRFFIKKYRADTNTQCHLFIDTSGSMNYSHTGSITKFQYA
                     KFLAASLAYLLNRQQDSVGLVAFTEKVHTHVPARSRTGHMRTIFGNLSLLEAGGETRL
                     AESLHQLAEILNRRGIIVLISDFYDQPERLQEAFQHLRFKGHDLVAFHVLDQNELDFE
                     FDDPVLLLEDSETQEQMPVLPDVVQKGYRERMRQHVEQMRTCAATNHVDYEQITTKQP
                     LDFALFSFLSRRAGR"
     gene            1682..>2616
                     /locus_tag="VFH91_02565"
     CDS             1682..>2616
                     /locus_tag="VFH91_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6861892.1"
                     /translation="MSFLTPLFFIGAAAVAAPILVHLVRRTRARRVLFPALLFVRQVP
                     QRTIRRRTLQNLLLLVLRCLAILLIVIAFTRPLFTSRAARNNNGAGATVILLDNSLSM
                     RRDQLFANAQARAKTAVGNAPSNEQLAVVTFGNRYNLVNRFSSDRNRSLSAIQSTSAG
                     WEGTNYEQALRGAESLLTELHTNGPRRIVMISDFQAPGWNQTNATFKLANDTQLTTLD
                     VRDTNPPANVAVTNVEARGVVFSQKYSDNLGVQISNFSETPRDHVEITFQLNDQTVEK
                     RDISLNSNDTKVVEFSNFNLNEGANRATIDIASND"
BASE COUNT          667 a          666 c          647 g          636 t
ORIGIN      
        1 acgaccattc cacgtactgg ctacgcaaaa tccaatcgag atggaaggca cctatccgct
       61 tcctgaggcg caattagatc gtttcatgtt tatggtcaaa attgggtatc tgcctgagga
      121 agacgaagtg gctgtcgtta aacagacgac ttcgaatcag gaaaccgagt ttgaacgcct
      181 gatgtcagcc gatgagattc tggcgtttca aaaattagtt aagcaggtgc ccgcatctga
      241 cactgtgacc cgttacgccg tgcgcctggt taacgcgagc cggccaaata ctccgagtgc
      301 tcctgacttc ataaacaaat gggtgacgtg gggtggttct ctacgtgctt ctcagttcct
      361 gatcctgggc ggaaaagcca gggccatact gagagggcgc tacaacgtgt cgtgcgagga
      421 tgttcgagca gttgcacccg ccgtgttacg tcatcgcgtg ttgctgaact ttcaggctga
      481 agcagaaaag gtcgactcgg atcaggtgat acaaaaatta attgaaacgg tgcccgaacc
      541 gaagagtgga ttgtgatgac cgagatcatt ttccatatat cattttccat ttgtcatttt
      601 ccattttgtg ttcaggaacg tctcagttga aagcatttag tagtaattga gtggtaagaa
      661 tgaataatga aaaatggaaa ttgatatatg caagatcctc tagcttttgg agaagcaata
      721 aggaggagcc gaagtcagca ccagtcattg ccccctccgc gactgagcct acaccgacga
      781 gatttttgga tcctgaacta ttggccagaa ttggctcgct ggaattgctc gctcgcgctg
      841 ttgttgaagg gttcatgtca ggcttgcatc gttcgccatt tacgggtttc tcaaccgaat
      901 tcaccgaata ccgtcagtac aacccgggcg acgatctgcg atatcttgat tggcgcttgc
      961 ttggtcgcac cgatcgattt ttcataaaga agtatcgagc ggatacgaat actcagtgtc
     1021 atctcttcat cgacaccagt ggttcgatga attactcgca caccggctca attacaaaat
     1081 tccagtacgc gaagtttctc gccgcctcac ttgcttacct tttgaaccgt cagcaggatt
     1141 cggttggcct cgtcgcgttt actgagaaag tacacacaca cgtgccggct cgaagtcgca
     1201 ctggtcacat gcgcacgatc ttcggcaatc tttcattgtt ggaagcaggt ggcgaaactc
     1261 ggctggcgga gtcattgcat caactggctg agatactcaa tcgacgcgga atcatagtct
     1321 tgatctcgga cttttacgat cagcccgaac gcttgcaaga ggcctttcag catctccggt
     1381 tcaaagggca tgacctggta gcgtttcatg tgttggacca gaatgaattg gattttgagt
     1441 ttgatgaccc cgtgctgctg ctcgaagatt cagagaccca ggagcaaatg ccggtattgc
     1501 cggacgtggt ccaaaaaggc tacagagaga gaatgcggca gcacgtcgag cagatgcgga
     1561 catgtgcggc tacaaatcac gttgattacg agcagataac gactaaacag ccgctcgact
     1621 ttgcgttgtt ttcgtttctc tcccgtcgcg ccggacgata aaaagggcta agcaccaatc
     1681 aatgtctttc ctaaccccac tcttcttcat aggggctgcg gcagtcgcgg cacccatact
     1741 tgtgcacctc gtcagacgca cgcgcgcaag aagagtgcta tttcctgctc tgctttttgt
     1801 tcgacaagtt ccccaacgca ctattcgcag gcgtacgctc caaaatttgt tgctgcttgt
     1861 gctccgctgt ctcgcaatac tcttgatcgt aatcgccttc acccggccgc ttttcacctc
     1921 ccgagctgcg cgaaataaca atggggccgg cgccaccgtg atactcctcg acaactcact
     1981 tagcatgcga cgtgatcaac tgttcgcaaa cgcacaagct cgggcgaaaa ctgccgtcgg
     2041 taatgcaccc tcgaatgaac agctggccgt ggtcaccttc ggcaatcgat ataaccttgt
     2101 taatagattc agcagcgata gaaataggtc gctgtccgca attcaaagca cttccgccgg
     2161 ctgggaggga acaaactacg aacaggcgct gcgaggagct gagtcgctgt taacggaact
     2221 tcacaccaat gggccaagac ggatcgtcat gatttccgac tttcaagccc cgggttggaa
     2281 ccagaccaat gcaacattca aactcgcgaa tgacacgcaa ctcaccacac ttgacgtgcg
     2341 cgacaccaat cctccggcta atgtggcggt cacgaatgta gaagctcgtg gagtcgtctt
     2401 cagtcagaaa tactcagata acctgggagt gcagataagt aattttagcg agactccgcg
     2461 cgaccacgtt gaaataacgt ttcagctaaa cgatcagaca gttgaaaaac gcgacattag
     2521 tctgaacagc aatgacacga aggtagtcga attctcaaat ttcaatctta atgaaggcgc
     2581 caatcgagct acgatcgaca tagcctctaa tgattt
//
LOCUS       DASSFP010000121         4752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_324532, whole genome shotgun sequence.
ACCESSION   DASSFP010000121 DASSFP010000000
VERSION     DASSFP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324532"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFH91_02570"
     CDS             complement(<1..812)
                     /locus_tag="VFH91_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET6861893.1"
                     /translation="MRAVIITKPGEVDVLEIREVNDLPKPTADRVLVRVHAAALNRAD
                     ILQRQGKYPPPPGAPTDIPGMEFAGEVSQAGPEVTSWKMGDRVFGICGGGAQAEFVTV
                     PSNHLARVPDNLSWTDAAAVPEVFITAHDALFTQAGLQPGETVLIHAAGSGVGTAAVQ
                     LVRSAGARSFGTSRSPEKLERAKEYGLNESMVVDGEPVQFVNALKAWTNKYGVDVILD
                     LVGAGYFEANLLALAPTGRLILVGTISGSRSTIDFGAVMSKRLTIRGTVLRGR"
     gene            917..1885
                     /locus_tag="VFH91_02575"
     CDS             917..1885
                     /locus_tag="VFH91_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HET6861894.1"
                     /translation="MPRPLIILNNSAARARRVWPMIKSELDAAKIEYDTHVTSAIGDA
                     TNRTRLAIHSGATLIVVVGGDGSLSEVAEGYFEFSSNPEHLPKPINKEVTLAVLPAGT
                     GDDFARGLVGKRAPLNAWIKDLLAKLTSNEEPATRFIDVLYGRCDDLQTPFICLNAST
                     MGIGGETASRVAAQGKFMRRFSGEFRFLFAALGALAVWRERRVKVSVDGKQISDGPMN
                     LVAVANALYAGGGMKLSPDARLDDGKLDVVTASGLSRSNVITELSRVHKGGHVHNPKV
                     NITQGEIVLIETLMVQDAMLIEADGNVRGVTPVRFEVIPSALQFMI"
     gene            complement(1979..2437)
                     /gene="rpiB"
                     /locus_tag="VFH91_02580"
     CDS             complement(1979..2437)
                     /gene="rpiB"
                     /locus_tag="VFH91_02580"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET6861895.1"
                     /translation="MKIAVGADHGGYPLNERVIEELKLHNHEVIDFGTHDGSKPDDYP
                     DYARKVAQAVQSGSAEIGILVCGSGVGAAVAANKVRGVRAALCGDTYSGHQCREHDDC
                     NVLCLGARVTGVELALEIVRAFVGASFTGEERHRRRLAKVAAMESEFDKG"
     gene            complement(2465..4501)
                     /gene="tkt"
                     /locus_tag="VFH91_02585"
     CDS             complement(2465..4501)
                     /gene="tkt"
                     /locus_tag="VFH91_02585"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6861896.1"
                     /translation="MSAPLQKENTDPKLDQLCINTIRTLTLDAVQKAQSGHPGLPLGA
                     APMAYVLWTRFLQHNPQNPKWENRDRFLLSAGHGSMLLYSLLHLTGYDLPLEELKHFR
                     QWGSRTPGHPEYGLTPGVEITTGPLGQGFANGVGMAMGAAHLAAKFNKPGFPVIDHHV
                     YAIVSDGDLMEGVASEAASLAGHLKLGKLIYLYDDNHVTIEGFTNLAFTEDVPKRFQA
                     YGWHTRTVDDGNNLEEIEAAISEARAIEDQPSLISVKTIIGFGMPTEGTRKAHSDPPG
                     EEAVRETKRQLGWPEDKQFFIPDEALTHFRKAVERGAQQEADWQSQVEQYRTQFADDG
                     KLLQMTIAGDLPQDWEKHLPSFEDAKPIATRVASGEVINALAQVMPMLIGGSADLGVS
                     NNTDIKGGGSFEAGEYGGRILHFGVREHAMGAALTGMSLNGGLIPFGGTFLTFSDYMR
                     ASIRLAALSEVQVVYVFTHDSIGLGEDGPTHQPIEQLAALRAIPHLYVVRPADPAEVS
                     EAWRLAILRRHGPTALALTRQKVAVIDRKKFAPAIGVRQGAYVLAEAETTDGHEVTPK
                     LLLLATGSEVSLALEARDSLQKDGIPTRVVSMPCLELFEEQSPEYRGKVLPISVGARL
                     AIEAGVRQGWDRYVGQGGDVIALDRFGGSAPGEIAMKELGFNLDNVLKRARALL"
BASE COUNT         1171 a         1286 c         1109 g         1186 t
ORIGIN      
        1 cggcccctca aaactgtacc tcgaattgtt aggcgtttcg acatcacggc accaaaatca
       61 atggttgatc tggagcctga aattgtacca accagtatca atcgtccagt cggcgctaac
      121 gctaataagt tagcttcaaa atagcccgcg cccacaagat caagaatcac atctacacca
      181 tatttattgg tccaagcctt aagtgcgttt acgaactgaa ccggctcacc gtcgaccacc
      241 atggactcgt tcaaaccgta ctcctttgct cgttctaact tttctggcga acgcgaagtc
      301 ccaaacgaac gcgcgccggc cgaacggaca agttgtacgg cagccgttcc tactcctgat
      361 cctgcggcat gaatcagcac agtttccccc ggttgcaaac ctgcctgggt aaataaggcg
      421 tcatgagctg ttatgaacac ctcgggaaca gcagcagcat cggtccaact caaattatcc
      481 ggcacgcgcg cgaggtgatt tgacggcaca gtaacaaatt cagcttgcgc accaccgccg
      541 caaatcccga atactcgatc acccatcttc caggaagtaa cttctggacc agcttgcgag
      601 acctctccgg cgaactccat acccggaatg tcagtcggag cgccaggcgg cggtggatat
      661 ttgccttgtc tttgcaagat gtctgcacga ttcaaggctg cagcatgaac gcgaacaaga
      721 actcgatcgg ctgtcggttt tggcagatcg ttaacctctc gaatctccag gacgtcaact
      781 tcgccgggtt ttgtgatgat tacggctctc attgtttgtt tttggaaact tagcttctat
      841 gttttactcc agttgcggac ataattacca agagcgaagc actaaagaca aacaaccaga
      901 ataaaaactc tctcagatgc cccggccctt aatcattcta aataattcgg cagcgagagc
      961 acgtcgtgtc tggcccatga tcaagtcgga attggacgcg gccaaaatcg agtacgacac
     1021 tcatgtcact tcagctatcg gagacgcaac aaatcggacc agacttgcaa ttcactctgg
     1081 agcaacactc atcgttgtag tcggcggaga cggtagtttg agtgaggtcg ccgagggcta
     1141 ttttgagttt tcgagcaacc ccgaacacct tcccaaacca ataaacaaag aggtcacact
     1201 cgcagtactt ccagcaggta ctggtgacga ttttgctcga ggattggttg gcaagcgagc
     1261 accgctcaat gcctggatca aagacttact cgcaaaacta acatcgaacg aggagcccgc
     1321 aacacgtttc atcgacgttc tttatggccg gtgcgacgat ttacaaacgc cgtttatttg
     1381 tttgaatgcg tcgaccatgg gcatcggcgg ggaaaccgca tcgcgtgttg ctgcgcaggg
     1441 taagtttatg cggcggtttt caggtgagtt tcgatttctc tttgcggctt tgggagctct
     1501 ggcagtgtgg cgcgagcgcc gcgtaaaagt ctccgttgat gggaagcaga tttccgatgg
     1561 accgatgaat ttagttgcag tagccaatgc gctttatgca ggcggtggta tgaagttatc
     1621 gccggacgct cgtttagacg atggcaaact tgatgttgta accgcgtcgg ggctttcgcg
     1681 ttcaaatgta attaccgaac tgtcgcgagt gcataaaggc ggtcacgttc acaatccgaa
     1741 agtaaacatc actcagggcg agatagtgct gattgaaact ttaatggtgc aggatgcgat
     1801 gctgatcgaa gctgatggaa atgttcgagg tgtgacgcca gtgcgattcg aagttattcc
     1861 ctcagcactt caattcatga tttaaaggct gcgctttgtg ctttgtactt ggttccaatg
     1921 tgtggatgtc aaatacctta attttaaatg agcatcgcta acgataagcg atcgataatc
     1981 agcctttatc aaactcgctt tccatagcgg caaccttagc tagccgtctg cggtgccgct
     2041 cttcaccagt gaaactcgcc ccgacgaatg cccgcacgat ctctaatgcg agttcaactc
     2101 cggtgacgcg agcacccagg cacaggacat tacaatcgtc atgttcccga cactgatgac
     2161 ccgaataagt gtcaccacaa agtgctgcac gtactccgcg aaccttattg gcagctacag
     2221 cggcgccaac accactgccg caaaccagta ttccgatttc tgcggatccg gactgaacag
     2281 cctgagctac tttcctggcg tagtctgggt aatcatccgg ttttgaaccg tcgtgagttc
     2341 caaaatcgat gacctcgtga ttatgaagtt ttagctcctc aattacgcgc tcatttaacg
     2401 gatagccgcc gtgatctgcg cctactgcga ttttcataaa cttccttact acttaagacg
     2461 cgctctaaag cagtgcgcga gctcgcttca aaacgttgtc gaggttgaac cctaattctt
     2521 tcatcgcaat ctcaccgggc gctgatcctc caaaccgatc caatgcgatc acgtctcccc
     2581 cttgaccgac gtaacggtcc caaccttgtc tcactccggc ctcaattgct aatcgtgcgc
     2641 caactgaaat cggcaaaact ttaccgcgat actcaggcga ctgctcttca aataactcca
     2701 gacaaggcat cgaaacaact cgagttggaa tgccatcttt ctgcaaacta tctctagctt
     2761 cgagcgccaa agagacctct gaaccggtcg ccaacaataa tagctttgga gtgacctcgt
     2821 ggccgtccgt cgtttcagct tcagccaaaa cgtaagctcc ttgccgcact ccaatggcag
     2881 gagcaaattt cttccgatcg ataacagcta ctttttgtct cgtcagcgcc agagcggtag
     2941 gaccgtggcg tcgtaggata gcaagccgcc atgcctcact tacctcagca ggatctgcgg
     3001 gtcgcaccac atatagatgc gggattgcgc gtaaagctgc cagttgctca attggctgat
     3061 gggttggacc atcctcaccg agaccaattg aatcatgcgt gaaaacatag accacctgaa
     3121 cctctgaaag ggcagcgagc cttatcgatg cccgcatgta atctgaaaat gtcagaaagg
     3181 tccccccgaa tggaatcaga cctccattca gagacatacc tgtcaaagcc gcgcccattg
     3241 catgttcacg caccccaaaa tgaagaatgc gcccaccgta ctcgccggcc tcgaaacttc
     3301 caccgccctt tatgtctgtg ttgtttgaga cgccaaggtc cgccgaccca cctatcagca
     3361 tgggcataac ttgagcaagt gcattaatga cttctccgct tgccactcta gttgcgatag
     3421 gttttgcgtc ttcaaatgac ggcagatgct tttcccaatc ttgaggcagg tccccggcta
     3481 ttgtcatttg cagtaatttc ccatcatccg cgaactgagt tctgtactgc tcgacctgtg
     3541 attgccaatc agcttcttgc tgtgctcccc gttcaacagc cttgcgaaag tgggtcaacg
     3601 cttcgtctgg tatgaagaat tgtttgtctt cgggccaacc gagttgtcgt ttggtttctc
     3661 gcactgcttc ttcgcctggc ggatcgctat gagctttgcg agttccttcg gtaggcatgc
     3721 caaatccgat aattgttttg acggaaatca gcgagggttg atcttcaatt gcccgcgcct
     3781 cggaaatagc tgcctcaatt tcttccaaat tgtttccgtc atctaccgtg cgtgtatgcc
     3841 aaccgtaagc ttgaaatctc tttggtacat cttcggtgaa ggcgagatta gtaaacccct
     3901 cgatcgtgac gtgattatcg tcgtagagat agatcaactt gcctagtttt agatggccag
     3961 ccaacgacgc ggcctccgaa gccaccccct ccatcaagtc gccgtcagag acgatggcat
     4021 aaacgtgatg gtcgattacc ggaaatcccg gtttgttaaa cttcgctgcc agatgggccg
     4081 cacccatcgc cattccgact ccatttgcga agccctggcc caatggacca gtggtgatct
     4141 ccacacccgg tgtcagtccg tactcgggat gaccgggtgt tcgggatccc cactgccgga
     4201 aatgttttaa ctcttcaaga ggcaaatcgt aaccggtcaa atgcaacaac gaataaagca
     4261 acattgatcc atgaccagcc gagagcagaa aacgatctcg attctcccat ttgggatttt
     4321 gcgggttgtg ctgaagaaat cgagtccata aaacgtaagc catcggagca gcacccagcg
     4381 gtaggccggg atgtcctgac tgagcttttt gaacagcgtc gagcgtcaat gttctaatag
     4441 tattgataca aagttgatca agtttcggat cagtgttttc tttttgcaga ggcgcactca
     4501 tttgatgatt tcctaaggag aattttttca accttcacgt gtttcggagt aatcatagga
     4561 aagacaagtc acaaaatctg tcttcaagca tcgcaatttc aacttcaatt cattcaccca
     4621 tcagacgacc ataaaactgt tcctgacgtg ccaaaccatc cgcgtatcga cggtgttgtg
     4681 acatatcagg ttcaaaaacc tgctcttcaa ctaccctcaa gtcttcgatg ggtcctattt
     4741 tgcccaccgc ct
//
LOCUS       DASSFP010000122         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1358095, whole genome shotgun sequence.
ACCESSION   DASSFP010000122 DASSFP010000000
VERSION     DASSFP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358095"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VFH91_02590"
     CDS             complement(<1..1229)
                     /locus_tag="VFH91_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861897.1"
                     /translation="MGLRPRFKTPLSQARDRFVNYFTGPFMSLRPRTRFLIGASVLVT
                     LTTLLLWNNSSSTITQNYKEGEVLSRAIVVPADITTVDLVETERRKAAAREAIRPIFN
                     FDSSRAETSVQGFRSDWEELKKQVNSGQAKTAVWSGDGGPGVGRAIIAHNFDDDDLDR
                     LTSILREIGSGYVYDDSEVDRLGQEIVLVDIRNPMAQLIMPAPRTRMSALSAARRNLE
                     LRVLNLPDWSGEEKLQLVAALAPLIRPNVVLDQTATAAARENEANNVPVVVISLKRNQ
                     ILAREGDTVTPNILAQVAAIKSTGHSGRPWQQFFGLLLVVIAVYWAGWKFTEHRSSRG
                     VLSLGKHKAFALVGSAILIQTALMRVGFTFGDSIAAWMSQSGPPFNDPAIWNFAIPFA
                     AAALLVAMLVDTQLAFLT"
     gene            complement(1229..2947)
                     /gene="hflX"
                     /locus_tag="VFH91_02595"
     CDS             complement(1229..2947)
                     /gene="hflX"
                     /locus_tag="VFH91_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET6861898.1"
                     /translation="MGNTLGLKPNQLRRIEKLYTRRIRPNQIVTPEFARQLSELSHET
                     RRQIGALIDRKGHVQYVMVGDNRGIELPDFKRVRVATGRFRGLRCIHTHLRGEALTQD
                     DLTDLALLRLDLMAAIDVDTDTGLPGMVHAAHLLPLHAGELDNAGMPGTFEFIEPQLA
                     GSLDLDFLSLIDSLEDEMARNRQATRRSELRDRTILVGVATNGMADAEESMAELSELA
                     ASAGVVVQDTIVQRRSAIDPRTVLGRGKLDELIIRALQLGADMLVFDRELSPAQVRSL
                     SEATDLKIIDRSQLILDIFAQRAQSKEGKIQVELAQLKYLLPRLIAGQDSAFSRLAGG
                     IGGRGPGETKLETDRRRVRDRINRLEKEIEVQRQRRQERRKGRTRQRLPIVSIVGYTN
                     AGKSTLLNALTKSAVHAEHRMFATLDPTSRRLRLPREQEVIINDTVGFIQALPPDLLS
                     AFRATLEEITDSSLLIHTVDISNPRWPQQVESVKRILSDLKLAEIPTLLVLNKADLLD
                     NDALQAAARQARQFEKSDPVVISALRPATLDRLLDRVGLLLARDLGLESESDLKHRHA
                     VISEVA"
     gene            3450..>3903
                     /locus_tag="VFH91_02600"
     CDS             3450..>3903
                     /locus_tag="VFH91_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861899.1"
                     /translation="MVGKIVRITPAYAIEGAEIAIDCTDLDTSIPSQCSVIIGSLEAP
                     IVALGPRRVLAIVPETRVEGSLEVRLRVDDELSPPATLVVGKRVITDVHPVTNPAFDP
                     DDDALFVTRSGSRGDQLPVTLFRVDTEGEASEYSGDVANPTGLAFDPEG"
BASE COUNT          907 a         1023 c         1012 g          961 t
ORIGIN      
        1 gtcaggaaag caagttgagt atcgacgagc attgctacga gcaaagccgc ggcagcgaag
       61 ggaattgcaa agttccagat agctggatcg ttgaaaggtg gcccagattg cgacatccag
      121 gcggcaatgc tgtcgccaaa ggtaaagcca actctcatca gcgcagtttg gatcaggata
      181 gcagaaccga ccagggcaaa cgctttgtgt ttaccaagtg ataatacgcc gcgtgaactc
      241 cgatgctcgg taaacttcca tcccgcccag taaacggcga tcactaccaa cagtagaccg
      301 aaaaattgct gccacggtct gcccgaatgt cccgtgctct taattgcggc aacctgggcc
      361 agaatatttg gagtgacagt gtcgccttct cgcgcaagga tctgattgcg ctttaatgaa
      421 atgactacca ccggaacgtt attggcctcg ttttcacggg ctgccgcggt agcagtctgg
      481 tccaaaacta cgttcggacg gatcaatgga gccaacgccg caacgagttg cagtttctcc
      541 tcaccgctcc aatcaggcag gtttaaaact cgtaattcca gatttctgcg ggccgctgag
      601 agggcgctca tgcgtgttcg aggagcaggc atgatcagct gtgccatggg attgcgtata
      661 tccaccagca caatttcctg gccgagtcgg tccacttcgc tgtcgtcgta aacgtaaccg
      721 ctcccaattt cccgaagaat tgatgtcaag cgatcgagat cgtcatcgtc gaagttatga
      781 gcaatgattg cgcgacctac acctggtccg ccgtcgccac tccaaacggc ggtctttgcc
      841 tggccggaat tgacctgttt tttcagttcc tcccaatcac tccgaaatcc ctggacggag
      901 gtttcggctc tactggaatc gaaattgaaa attggccgga tagcttctct cgccgcagct
      961 ttgcgacgtt cggtttctac caggtcgacc gtagtaatgt cggcaggcac tactattgcc
     1021 ctggaaagta cttcgccttc tttgtaattc tgggtaattg tcgaagagga gttgttccag
     1081 agtagcagtg tagttaatgt gaccagaacc gaagccccga tcagaaatcg tgtgcggggt
     1141 ctcaacgaca tgaacggtcc agtgaagtag tttacgaacc gatcgcgtgc ctgacttaag
     1201 ggagttttga atcgtgggcg aagtcccatt acgcaacttc ggagataaca gcatgccgat
     1261 gctttaaatc cgattcactt tctagtccaa gatctcgagc caataacaaa ccaacacgat
     1321 caagcagtcg gtctaacgtt gccggcctca gggcagatat gaccacggga tcactcttct
     1381 caaactgtct ggcctgtcga gcggcagcct gtaacgcatc gttgtctagt aagtcagctt
     1441 tattcaatac cagcaaggtt ggaatctccg cgagtttcaa gtcggagaga atacgtttta
     1501 ccgattcaac ctgttgcggc caacgcggat tcgaaatatc aacagtgtga attaataagc
     1561 tgctgtcagt tatctcttcc agcgttgccc gaaacgcaga gagcagatcg ggcggaagcg
     1621 cctgaatgaa tccgaccgtg tcgttaatga ttacttcctg ctctcgaggc aaacgcaatc
     1681 tgcgcgaagt tggatccagc gttgcaaaca ttcggtgttc tgcgtgaacg gcactcttag
     1741 taagtgcgtt cagcaaggtt gatttccccg catttgtgta accaacaatc gaaacaatcg
     1801 gcagtcgctg ccgcgttcgt ccttttcgcc gctcctgtct gcgttggcgt tggacctcta
     1861 tttctttctc gagccggttg attcgatctc taacgcgacg tcgatcggtt tccagcttgg
     1921 tttcacccgg tccacgacca ccgattccgc cggccaaacg ggaaaacgca gaatcctgac
     1981 cagcgataag tcgtggtaac aaatatttca actgtgccag ctcaacctgg atctttcctt
     2041 ctttcgattg agctcgctgg gcaaagatgt cgagaattaa ctgtgaacgg tcgatgattt
     2101 ttaggtcagt cgcttcggaa agagatctta cttgtgctgg actaagctcg cgatcgaaca
     2161 ccagcatgtc ggctcccagt tgcagggccc ggattatcag ctcatccaac tttccacgcc
     2221 ccagaaccgt tctcggatcg atagccgatc ggcgctgaac aattgtgtcc tggacaacca
     2281 ctccggctga ggcagcgagc tctgagagtt ctgccatcga ttcttcagca tcagccatgc
     2341 cgttggtggc gaccccgaca agaattgttc ggtcgcgtaa ctccgagcgg cgagtcgcct
     2401 ggcggttgcg ggccatttca tcttcaagtg aatcgatgag cgaaagaaaa tcaagatcaa
     2461 gactgccggc taattggggc tcgataaatt caaaggttcc gggcatacct gcgttgtcca
     2521 attcacccgc gtgtaatggc agcagatgtg ccgcatggac cataccaggt agtcctgtgt
     2581 ctgtatcgac gtcaattgcc gccattaaat cgagtcgcag taacgcgagg tcggtcaaat
     2641 cgtcctgggt cagcgcttct cccctgaggt gggtatggat gcaccgcagg cctcgaaatc
     2701 ggccggttgc gacgcgtaca cgtttgaagt ccggtaattc aatacctcga ttgtcgccaa
     2761 ccatgacgta ctgcacgtga cccttacgat caatcagtgc accgatttgt cttcgcgtct
     2821 cgtgcgataa ctcgcttaac tgccgggcga actcgggcgt aactatttga ttcggccgta
     2881 tacgccttgt atagagcttt tcaattcgtc gaagctggtt tggctttagg ccaagtgtat
     2941 tgcccaatgc gctagtgatg tccctgtcct ccaatacttg agacgttgaa cattgttagg
     3001 ggcttaaatt gaaatggcgg cgccgatgag ttacaggatg agtactgcga gcggtgtttt
     3061 gcaggccctg atgagaagct ggtgttgttg ctcggcgaaa tcgcgtcgct gagtccctta
     3121 agcattagac tttctgtggt gcgtaacttc ctatcaacct caatcttgga tatgtcaatc
     3181 gttcagttcg cacatgcttt tgtccagggt gcgaattata ccatagtgac gatcctgaac
     3241 agccaatcgg acgctccgtt tatttcgatg aggcaaacct cgcccgggtt tgtaactttt
     3301 aattacagtg ctgcctattg tcattcatac cgccccggga aagagtccaa ttggtgtcgt
     3361 tattagacat caattttttc gaggcgtatc atgaacgacc tcacgaatac gacaaagtga
     3421 actcgggtcg aagggaatgc cttttcggaa tggttggaaa aattgttcgg atcactcctg
     3481 cttacgcaat cgaaggcgct gagattgcca tcgactgcac tgatctcgat acgagcatcc
     3541 cttcacagtg ctctgtaatc atcggaagtc ttgaagcccc gatcgtagcg ctgggacccc
     3601 gccgcgtgct cgcgattgtc cctgaaacac gcgtggaggg atccctggaa gtcagactta
     3661 gggtcgacga cgaattaagc cctccagcca ccttggtggt cgggaaaagg gtaatcactg
     3721 acgtccaccc ggttacgaat cctgccttcg atccagacga cgacgcgctt tttgtaacgc
     3781 ggtccggttc aagaggcgat cagctgccag tgaccctatt cagggtggac accgaaggag
     3841 aggcttcaga atactcaggc gacgtagcga atcccaccgg cttggcattt gacccggaag
     3901 ggc
//
LOCUS       DASSFP010000123         5360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1514393, whole genome shotgun sequence.
ACCESSION   DASSFP010000123 DASSFP010000000
VERSION     DASSFP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514393"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFH91_02605"
     CDS             <1..722
                     /locus_tag="VFH91_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861900.1"
                     /translation="LQPQSVRERIALAMFFSNHSLTEQAIPMMREANQLPAEEKLSVL
                     QELLDTNRLVEAYEVWLSGSEKGSSDQLNRSASITDGGFEGNLTIDDPGFGWRFNSQF
                     RTVNASLDQIEPHSGKQSLRLEWHGDPDHSAPIVKQLVLTAPRTRYRLKFASRTQSLT
                     TICPPVITITSRKGDAEILLGQSPTLQQGSSEWQNFSVDFSTTDSPQAVWISITRIAG
                     ADGSCPIFGTLWIDDVSLAKI"
     gene            833..2998
                     /locus_tag="VFH91_02610"
     CDS             833..2998
                     /locus_tag="VFH91_02610"
                     /inference="COORDINATES: protein motif:HMM:NF018905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HET6861901.1"
                     /translation="MNTLVVYLELLCTAVLLMTFQSPVGSAAKIEQESQHASQNSVAG
                     ARVLTVAARSSAATTSSSSPVSSPFQSAGTWSSPIPLPIVPIHMHLLPNGKVLIWQDD
                     NNPNYNIDGSRGPGSTLAYVWDVSVGALTQVSDTTDDLFCSGHSFLPDGRLLITGGHI
                     SDNVGIRTTTIFDYRSNSWSTGQLMNAGRWYPTNTTLGNGEVLVVSGDIDRTQGVNTL
                     PQVWQTTGGWRSLTTALRSLPLYPMMHLAPNGMVFNSGPNADTGYLDTTGTGSWTPVA
                     NHNYVYIRDTGSSVMYDDGKILVVGGGDPPTATAEVIDLNAANPSWQDVAPMGNPRRQ
                     MNATLLPDGMVLVTGGTSSGGWNDATNAVRAAELWNPSTNTWTQLASMQNRRLYHTTA
                     ILLPDGRVICAGGGRPNPTGDTSHPDAEIFSPPYLFNGGRPSINSAPDNIAYGQTFSI
                     STPNAGSISKVTLVALPSVTHAFDMNQRFKSLSFSVSRKALSVVGPANGNVCPPGYYM
                     LFILNSNGVPSIAKIISIGPSSPPSPPAVPVSLVANAGSSDQINLSWTDSSNSEDGFR
                     IEQCQAAGCTNFAEIATVGANVTNYSNSGLTPATTYQYRIRAYNSTGNSGYSNVASAS
                     TGVLILAPSNLTGTAVSGTQINLNWSDNSDNEVGFRIERCVNQNCKNFSEITTVEANV
                     TAYSDSNLKAGRTYRYRVRGYAPNSTSPYSNIANITTNR"
     gene            3031..4152
                     /locus_tag="VFH91_02615"
     CDS             3031..4152
                     /locus_tag="VFH91_02615"
                     /inference="COORDINATES: protein motif:HMM:NF023375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3466 family protein"
                     /protein_id="HET6861902.1"
                     /translation="MRAVDLYRVVLVLVFSASLSFISESSVNAQLRTYTVTDLGTLGG
                     QRSAAFSINNSGQVVGNAETASNESHAFLYEANTLIDLGTLGGKESYAYSISDSCLIV
                     GRSQNEAGFYRAFITTCAADSPLVDLSSVDSSLRGHYSTASSVNKAGRTVGYTVTAAG
                     SHQGGRSRSFLFSNNRIVDLGTLGGEESVPTAINDAEQIVGYVSREPHATYADHRAFL
                     YQNGRIVDLGTLGGRMTTPLAMNSSGAVVGNAQTSGGLPRAFIYNGRLQGLGTLPGGS
                     QSMAFGINNLGQVVGSSDNQTRTLHAFLYSKGTIKDLNDLIPTFSGWILVEARSINDS
                     GQIVGNGILRGQERGFLLTPVDLAQADTAIGFTLPKIVP"
     gene            4162..5082
                     /locus_tag="VFH91_02620"
     CDS             4162..5082
                     /locus_tag="VFH91_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6861903.1"
                     /translation="MNGEASILVDSVGPSTMLPDSTGASVEEQDWTLIIRPKARWLDL
                     HLVDLWRCRDLVMLFVRRDFVSFYKQTILGPLWFIIQPLLTTLTFTLIFGNIAQLSTD
                     GLPKILFYLSGVTAWNYFSDCLLKTSETFTANSNLFGKVYFPRLAVPLSIVISNLIKF
                     GIQLGLFLGFYLYFFGKGANVHPTSALLLMPLLLVLMAGLGLGSGIIVSSLTIRYRDL
                     RFLVQFGAQLLMYSTPVIYPLSKLPQQYRWIMLANPMTPVIEIFRYAFLGTGTCSWKL
                     LGFCAAATSLILTIGILLFNRVEKTFMDTV"
     gene            5120..>5360
                     /locus_tag="VFH91_02625"
     CDS             5120..>5360
                     /locus_tag="VFH91_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861904.1"
                     /translation="MSNHTAVHINHLSKQYRLGVMGGRRLVDDVNQWWAKIRGKPDPL
                     ERIGSRNNGNGAVKNANTGDKLLALNDVSLNIKQGE"
BASE COUNT         1306 a         1402 c         1325 g         1327 t
ORIGIN      
        1 ctctccagcc acaatcggtg cgcgagcgga ttgcactcgc aatgttcttc agtaaccact
       61 cgcttacgga acaggcgatt ccaatgatga gggaagcaaa ccagcttccg gcagaagaaa
      121 aactgtcggt gcttcaagaa ctgctcgata ccaataggtt ggtggaagct tacgaagtct
      181 ggctatccgg aagcgagaaa ggctcctctg atcagttgaa ccgatcagcg tcaatcactg
      241 atggaggctt tgaaggaaac ttgacaattg acgatccggg ttttggttgg agattcaact
      301 cccagttccg aacggtgaac gcgtcgctag atcaaattga accacactca ggcaagcaga
      361 gtttgcggct tgagtggcac ggcgatcccg accactcggc tccaattgtg aagcagcttg
      421 tcctgactgc gcccaggact cgttaccgcc ttaaattcgc atcacgcacg caatcactca
      481 ccactatttg tccaccggtt atcaccatca cctcgcgcaa aggagatgca gagatcctgc
      541 tgggccagtc accaactctc cagcagggaa gtagcgaatg gcaaaacttc tccgtcgact
      601 tctcgacgac tgattccccg caagccgttt ggatcagcat cacccgtatt gcaggagctg
      661 acggttcgtg tccgatcttc ggcactttgt ggatagacga cgtctcgctc gcaaaaatat
      721 gagttggtaa gcctacgttt ttgaagttag ctggtaatcg cgctatcaat ccgcgcgttt
      781 cccagcaccc gcagtgtctt ccaatatata caacgttacc gagggttcaa tcgtgaatac
      841 actggtggtc tatctagagc tgctctgcac ggcggtctta cttatgacgt tccaatcccc
      901 agttggatcg gcggccaaaa tcgaacaaga gagtcaacac gcttcacaga actctgttgc
      961 tggggcccga gtgcttactg tagcagcgag aagtagtgcc gcgacgacct cgagctcaag
     1021 tcccgtaagc agtccatttc agagcgccgg cacgtggtca tctcctattc cgttaccaat
     1081 cgtacccatc catatgcact tgctgcctaa cggtaaggta ctgatttggc aggatgacaa
     1141 caatccgaac tacaacatcg acggctcccg tggccctggg tcaacactgg cctacgtgtg
     1201 ggatgtttca gtaggagcgc tcacccaggt tagcgacacc acggatgatc ttttttgcag
     1261 cggccattct tttcttccgg acggacgact gctaatcaca ggaggacaca tatccgataa
     1321 tgtgggaatt aggaccacca ccatattcga ctaccgttct aacagctggt cgactggaca
     1381 attaatgaat gccggtcgct ggtatccaac caacaccacg ttaggaaatg gtgaagtact
     1441 ggttgtttca ggagatatcg ataggaccca aggtgtgaac acgttgcctc aggtttggca
     1501 aacgactggc ggctggcgct ccttaactac agctctccga agtttgcctc tttacccgat
     1561 gatgcatcta gcaccgaacg gtatggtctt caattccgga ccaaatgcgg ataccgggta
     1621 tctcgacacc actggaaccg gttcatggac tccagtggcg aatcacaact acgtatacat
     1681 ccgagatacc ggctcatcgg taatgtatga cgatggcaag atccttgtgg taggcggcgg
     1741 tgaccctccc actgccaccg ctgaggttat agatctaaac gcagccaatc cgtcctggca
     1801 agacgtagct ccgatgggta atcccaggcg tcaaatgaat gcgacacttc tgccggacgg
     1861 tatggttctt gtgactggtg gcaccagctc tggcggatgg aacgatgcta ctaacgcggt
     1921 acgcgcggcc gaattgtgga atccgtcgac caacacctgg acccagttgg ccagtatgca
     1981 gaaccgccga ctttatcata cgacggctat tctattacca gacgggcgcg ttatatgcgc
     2041 cggcggaggc cgtcctaacc cgacgggaga tacatctcat cccgatgccg agatcttttc
     2101 gccaccttac ctttttaatg gaggccgtcc cagcatcaac tctgctccag acaacatcgc
     2161 gtacggtcaa acgttttcaa tatcaacacc gaatgcaggg tctatttcca aagtcacatt
     2221 ggttgctctt ccttcagtta ctcacgcgtt tgatatgaat cagcggttta agagtctgag
     2281 tttttcagtg tcgcgtaaag ctctaagtgt tgttggacca gcaaacggga acgtctgtcc
     2341 tcctggttac tatatgctct ttattctcaa cagcaacggc gttccatcca tagcgaagat
     2401 catttcaata gggcccagca gcccaccatc gccccctgca gttccagtta gcctggtagc
     2461 aaatgccggc tcgagtgacc agatcaatct ctcgtggact gacagttcaa acagtgaaga
     2521 tggattcagg atcgagcagt gccaggctgc gggctgcacg aactttgccg agatcgcgac
     2581 ggttggcgca aatgttacca actattccaa ctcaggtctg actccagcca cgacatatca
     2641 gtaccgaata cgcgcctata acagcactgg caactccggc tattccaacg tggcaagtgc
     2701 ctctacagga gtgttgatcc tggcaccttc gaacttgacc ggaacggcgg tctcaggcac
     2761 gcaaataaac ctgaactgga gcgataactc agataacgag gttggtttca ggatcgagcg
     2821 ttgtgtgaat caaaattgta aaaacttttc agaaatcacc acagtcgaag caaatgtaac
     2881 tgcatacagc gatagcaacc ttaaagccgg tagaacgtat cgatatcgcg tacgcggata
     2941 tgctccaaac agcacctcgc cctactccaa tatagcgaac atcacaacca acagatgagt
     3001 atcagagtta gtccagttaa ggaagtcgtc atgagagcag tcgatcttta cagggtggtt
     3061 cttgtattag ttttttctgc ttcgctaagt tttatcagcg agagttcggt aaacgctcag
     3121 ctacgaacgt ataccgtgac cgatcttggt accctcggcg ggcaacgcag cgcggcgttt
     3181 agtatcaata actcgggtca ggttgtaggc aacgccgaaa ccgcgtctaa cgagtcccac
     3241 gctttcctct acgaagccaa taccctgatt gacttgggta ccctgggagg taaggagagc
     3301 tatgcttact ccataagtga ttcctgcctt attgtaggcc gttcacaaaa cgaagccgga
     3361 ttctaccgcg cttttataac aacttgtgcg gcggacagcc cgctggtcga tcttagctca
     3421 gtcgattctt cactaagggg acattacagt actgcatcca gcgttaacaa agcaggacgg
     3481 actgtcggtt atacggtaac ggccgcaggt agtcatcaag gcgggcgaag ccgttcattt
     3541 ctttttagta ataatcggat cgttgatctc ggaacgctcg gaggcgagga aagcgttccc
     3601 acagccatta atgatgccga acaaattgtc gggtatgtca gccgcgaacc gcacgcgact
     3661 tatgctgacc atcgcgcctt cttgtatcaa aacggcagga tagttgactt agggactcta
     3721 ggcggtcgga tgacgacgcc cttagctatg aacagttctg gcgctgttgt cggaaatgcg
     3781 caaacatccg ggggattacc gcgcgcgttt atctataatg gccgccttca aggtctaggg
     3841 acgctccctg gcggcagcca aagcatggcg tttggcataa acaacttggg ccaagtagtt
     3901 ggctcatctg acaatcaaac acgaactctt catgctttcc tgtacagcaa aggaacaatt
     3961 aaagatctga acgatctaat tcctacattc tccggctgga tcttggtgga ggcgcgtagc
     4021 atcaatgact ccggccaaat tgtcggcaac ggtattctac gaggccagga acgtggtttt
     4081 ctccttacgc cggttgattt ggcgcaagca gataccgcga tcggctttac gctacctaaa
     4141 atcgttccat aacaggttca tatgaatggt gaggcatcaa ttctagtcga cagtgttggt
     4201 ccatctacga tgctcccaga ctccaccggc gcgtcggtcg aggagcaaga ctggacttta
     4261 attatccggc ctaaggcgcg atggctagac ttgcacctcg tcgatctgtg gcgatgccgg
     4321 gatcttgtaa tgcttttcgt ccgtcgcgat tttgtttcgt tctacaaaca gacgattctt
     4381 ggtccactat ggttcatcat tcagccgctg ctaacgacgc tgacgtttac gttgatcttt
     4441 ggaaacatag ctcagctttc aacggatggc cttcccaaga tcctgtttta cctatccggg
     4501 gttacggcct ggaactattt cagcgattgt cttctgaaga cctcggaaac attcacagcc
     4561 aactcaaatc tctttggaaa ggtttacttc ccacggctgg ccgtcccttt gtcgatcgtg
     4621 atttctaatc tgatcaaatt cggcatccag cttggtcttt ttttggggtt ctatctttat
     4681 ttctttggga aaggggctaa tgttcatcct acttcagcat tgctgctaat gcctctcctt
     4741 cttgtgctga tggcaggtct cggtctgggc agcgggatca tcgtctcttc gttaactatt
     4801 cgttatcgag atctccgctt cctggtccag ttcggagctc agttgttgat gtactcgaca
     4861 ccagttattt acccgctctc caaactgccg caacaatacc gatggattat gctggcaaac
     4921 ccgatgacgc cggttataga gatattccgt tatgcatttc tgggcaccgg cacctgcagt
     4981 tggaagctac tcggcttttg cgccgccgcc acgtcgctga ttctcactat cggtattctc
     5041 cttttcaaca gagttgagaa gacgtttatg gatacagttt gagaaattgg tagtgagaca
     5101 tcaggattaa ctcttataag tgtctaatca taccgcagta catattaatc acctctcaaa
     5161 gcagtaccgg ctcggagtta tgggtggcag acggctggtt gatgatgtca accaatggtg
     5221 ggcaaagata aggggtaaac cggatccact cgaaagaatc ggttcgcgaa ataacgggaa
     5281 cggtgctgtg aagaatgcga acactgggga caaactcctc gcgctgaatg atgtttctct
     5341 caacatcaaa cagggcgaag
//
LOCUS       DASSFP010000124         4166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_619924, whole genome shotgun sequence.
ACCESSION   DASSFP010000124 DASSFP010000000
VERSION     DASSFP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619924"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1344
                     /locus_tag="VFH91_02630"
     CDS             133..1344
                     /locus_tag="VFH91_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6861905.1"
                     /translation="MIKSLDGGEFEGYLSVPASGLGPGIVVLQEIFGVNAYMRSVVDW
                     YAAHGFIAVCPDLFWRQQPGVELTPDESGRPQAMKLYQGLDRSKAVEDSAAALEFLRQ
                     HPSCNGRVAAVGFCLGGNLAYLLSTRFNPDAAVGYYGVGIENFLDEAPNLKAPLMLHI
                     GEKDQSCPPEAQAAIHRVLDPNPLLTIHDYPGRGHAFGRPGGETYHPADAELANLRTI
                     EFLIHHLSGAGVEIPRKSLSEKWDEHVKYEFATRNTEDTLNTMVSDSYVNHVPVMTGG
                     VGQEELRAFYSTRFIPQMPPDTRMTPVSRTIGVDQVVDEMVFEFTHTIKMDWMLPGVE
                     PTGKHVKVALVVIVHFRNGKLAHEHIYWDQATVLAQLGLIDASKLPVIGVESAEKVLD
                     PSLPANTLMTR"
     gene            1438..2847
                     /locus_tag="VFH91_02635"
     CDS             1438..2847
                     /locus_tag="VFH91_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6861906.1"
                     /translation="MRRDLGTLESYATLIGILIGAGIFQVTSTAWLLTGPSVILGYLV
                     LTPAILATSIPYAAYLSTPLGNEPGGEYVHISRSVNGFRIAYIGAWLKIISYIGAGAY
                     LANALAGYIIALSGARMDADTFRLPLALAALCLFYLIHVIGIKWFGRFQVIMSTLKVL
                     ALLVLIIPGLFVVKLSNYTPFLTHGLSGFGFSLLPLFFAYAGFESLAQTAGEVRNSTK
                     RLPGILLKGITFTAVLYVLTSIVSFGVLPAGKIQSTSAPMAEVASLYLPGGAAIFVTV
                     GAIAAIMTALNGTMFVPSRLALMFVKDGLAPRWLGFVNESTATPIRALTLTLVGCIVL
                     LVSGQLSLALNVAVFALVVLYFIHSLVFLFLPRLNPVLASEITINIPRWLSQTAAVFS
                     VISMGVLIIIQLFQDFAVLSSTTFGQRMTEHSLTSVELIAIWGVVGAVLYWLARVNSN
                     GTKRLRPVSEITSGNSNGN"
     gene            2837..3559
                     /locus_tag="VFH91_02640"
     CDS             2837..3559
                     /locus_tag="VFH91_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6861907.1"
                     /translation="MEIEGTKVLVTGAARGLGRELINAFLAANVNQVFAGIRDENQID
                     HIQSQDSRVRAVLLDVTNETHIANLAHLEPFDILVNNAGVASFGNPLTKNFDEIRTEM
                     EVNYFGTLRVSRVIAPKMIERRRGVIVNVATAFARINLPMIGTYCASKAALLSLGQAL
                     RAYLVDYGVSVITVMPTTIDTDMSRGADVPKMTTTFVASEILSAIREEKHDPPIGDEA
                     HGVLERLHQDPQGLEQFLIQYR"
     gene            3568..>4166
                     /locus_tag="VFH91_02645"
     CDS             3568..>4166
                     /locus_tag="VFH91_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6861908.1"
                     /translation="MTETEINRQREEFPTLELGIHLLSHSLGPVPRAVRESMLSYCDA
                     WEHHTGEDAWATSWWELSRRVGDRIARILGGVSGSVQIQPNASIGLATAASCFDFKSG
                     KRRKVVTTELDFPSMEYIWDGQRHIGAQVEVVPSSDGISVSLERILDAIDEQTCLVAL
                     SHTSYRSSHRIDASAIVERAHSKGALVLLDVYQSAGVVPL"
BASE COUNT          957 a         1020 c         1070 g         1119 t
ORIGIN      
        1 agggtgttct agtaacgaat ctatgtaatc gtcgtaatct gtggattgta tcttgagttt
       61 tcacaccgtc atggaaaatg attctcttct ttaccattcc ctcttttccg tcttaccatc
      121 gtcgccatgg ccatgatcaa atcactcgac ggcggcgaat ttgaaggtta cctgtcagta
      181 ccagcctcag gacttggacc agggatagtt gtcttgcagg aaatcttcgg tgtgaacgcg
      241 tacatgcgca gcgttgtcga ctggtacgcg gcccacggat tcatagcagt atgtcctgac
      301 ctgttctggc gccagcaacc gggagttgaa cttactcctg atgaatcagg acgaccccag
      361 gcgatgaagc tatatcaggg gcttgatcgc tcaaaggcgg tcgaggattc tgcggctgct
      421 ttggaattcc tgcggcaaca cccttcgtgc aacggtcgcg tagctgcggt aggattctgc
      481 ctcggcggaa atctagccta cctcctgtct accagattca accccgatgc cgccgtcgga
      541 tactacggtg ttggcattga gaactttctt gatgaggcgc caaacctgaa ggctccgctg
      601 atgctgcaca ttggtgagaa agaccaatcc tgtcctcctg aagctcaagc ggcgatccat
      661 cgcgtactcg atccgaatcc gcttcttaca attcacgatt accccggaag aggtcacgca
      721 tttggcagac cgggtggtga gacttaccac ccggctgatg cggaactggc caacctcaga
      781 acgattgagt ttctgattca tcatctttcg ggcgccggag ttgaaatccc acgcaagtcg
      841 ctttcagaaa aatgggacga gcacgtcaaa tacgaatttg cgacccgtaa cactgaagac
      901 accttgaaca cgatggtctc ggattcatat gttaatcatg ttccggttat gaccggcggt
      961 gttggtcagg aagaattgcg agccttctat tccactcgat ttatccctca aatgccacct
     1021 gatacaagaa tgactccggt ttcacgcact atcggagtcg atcaggtagt tgacgagatg
     1081 gtcttcgagt tcacgcatac tattaaaatg gattggatgt tgcccggagt tgagccgacg
     1141 ggtaaacatg tcaaagttgc cctggtcgtt atcgttcact tcaggaatgg caagctggcc
     1201 cacgaacata tttattggga ccaggcgact gtactggcac aattaggctt aatcgacgcg
     1261 tcgaaacttc ctgttattgg agtagaaagc gccgaaaaag ttctcgatcc ttctttgcct
     1321 gctaacactt tgatgacgcg ctgaccttta gcaggtgcag tcacaaatgt tcgactactg
     1381 ctgaagcagt cgggtgacac gaaaatttcc gcgataaacc agaacccagt tggtcgcttg
     1441 cgtcgcgatc taggtacgct tgaatcatac gcaaccctga tcggaatact aatcggggcg
     1501 ggtatttttc aagtaacaag tacggcctgg ttgcttactg gtccaagtgt gattctgggt
     1561 tatctcgttc tcactccagc tatcctggcc acctcgattc cttacgcagc ttatttgtcg
     1621 accccacttg gaaacgaacc tggtggagag tacgtacaca tatcgcgcag cgtaaacggg
     1681 tttaggatcg cttatatcgg agcatggctg aagattattt cgtacatcgg cgctggcgct
     1741 tatttagcga atgcgctggc gggctacatc attgcactca gtggtgcacg aatggatgcg
     1801 gacacatttc gtttaccgct cgcactggca gcgctttgtc tcttttattt gatacatgta
     1861 atcggcatta aatggttcgg acgttttcag gtgatcatga gcacactcaa agtgctcgca
     1921 ctcttagtgt tgattattcc gggacttttt gtggtcaaac tctctaacta cactccgttc
     1981 ctaacacacg gtctcagcgg atttggcttc agcctgcttc cgctattttt tgcgtacgcc
     2041 ggtttcgaat cgcttgctca aactgcaggc gaggtaagga acagcaccaa acgattgcct
     2101 ggcattctgt taaagggcat tactttcaca gcggttctat acgttttgac atctatcgtt
     2161 tcgttcggtg ttttaccggc tggcaaaatt caatccacca gtgcgccgat ggctgaggtt
     2221 gcttcgcttt atcttcccgg aggcgcagca atatttgtca cggttggtgc aattgcggcc
     2281 atcatgacag ctctgaatgg aactatgttt gtcccgtccc ggctggcgtt gatgtttgtc
     2341 aaagacggac tggctccgag gtggctgggt ttcgtcaatg aatcaactgc gactccaata
     2401 cgcgcgttaa ctttgacatt agtaggctgc atcgttctgc tggtcagtgg acaactgtca
     2461 cttgctctga atgtcgcagt ctttgcattg gttgttttat acttcattca tagtttggtc
     2521 ttccttttct tgcctcgctt gaatccggta cttgcaagcg agatcacaat caatattccc
     2581 cgttggcttt ctcagacggc tgctgtcttc tcagttatct caatgggggt tttaataatt
     2641 attcagttgt tccaggactt tgccgtgtta agttcgacta ccttcggcca acgcatgact
     2701 gaacattctt tgacaagtgt tgagttgatc gcgatatggg gtgttgtcgg cgccgtcttg
     2761 tattggctcg ctcgagtaaa ctcgaatgga acaaagagat tgcgacccgt gtctgaaata
     2821 acgagtggaa acagcaatgg aaattgaagg gacaaaggta cttgtaacag gagcggcgcg
     2881 aggtctgggc cgagaactta tcaatgcctt ccttgctgcg aacgtgaacc aggtctttgc
     2941 cggcattcgc gatgagaatc aaatcgatca cattcaatct caggattctc gagtgcgagc
     3001 ggtgctcctg gatgtgacaa atgaaactca tattgccaat ctcgcacacc tggaaccctt
     3061 tgatattctc gtcaacaacg caggtgtcgc cagtttcggt aatcctttga caaagaattt
     3121 cgacgagatc cgcacagaaa tggaagtaaa ttatttcggt actctacgcg tatctagagt
     3181 aattgctcct aaaatgattg agcgacggcg gggcgtgatt gtgaatgtcg caacggcatt
     3241 cgccaggatc aacctgccta tgatcgggac ctactgcgca agtaaagcgg ctctgttatc
     3301 gttgggccag gcgctacgtg catatctcgt cgattacggt gtaagtgtta taactgtaat
     3361 gccgacaact attgataccg atatgagccg gggggccgat gtgcctaaga tgacgacaac
     3421 attcgtcgcc agcgaaatcc tctcggcaat tcgtgaggaa aaacacgatc cgccgatcgg
     3481 agatgaagcc cacggtgtac ttgagcgctt acatcaggat ccccagggtc tggagcagtt
     3541 tctaatccag taccggtgac atttaagatg actgaaacgg agatcaatcg gcaacgcgag
     3601 gaatttccga cgcttgaact gggaatacat cttctcagtc acagtcttgg gccagttccg
     3661 cgtgctgtcc gagagtcgat gctttcttat tgcgacgcgt gggagcatca cactggagag
     3721 gatgcatggg ccacgagttg gtgggagctt tcgcgccggg taggtgatcg aatcgctcgt
     3781 attctcggag gcgtctcggg gtcggtgcag attcagccca acgcatccat cggtttagcc
     3841 actgcggctt catgttttga cttcaaatca ggcaaacgca gaaaagtagt cacgaccgaa
     3901 ctcgatttcc cgtcaatgga gtacatctgg gacggccaga gacatatcgg cgctcaggtt
     3961 gaggtcgtgc cgtcgagcga tggtatttct gtatctttag agcgaattct tgatgcgatc
     4021 gacgagcaaa cttgccttgt tgcgctgtcg cacacgtctt atcgaagctc acatcgaatc
     4081 gatgcttcgg ctattgtcga acgcgcacac tcgaaaggtg cgctggtttt gctggatgtc
     4141 tatcaatcgg ccggagtagt accgct
//
LOCUS       DASSFP010000125         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_178158, whole genome shotgun sequence.
ACCESSION   DASSFP010000125 DASSFP010000000
VERSION     DASSFP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178158"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFH91_02650"
     CDS             <1..273
                     /locus_tag="VFH91_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HET6861909.1"
                     /translation="AADAMVKAANVRLVGWEKIDAGLVTAIVRGDVAAVRSAVDAGAA
                     AGKRVGEVVATHVIPQPHSDVDQGLPVLHTGETTKKKISGSVRGKS"
     gene            377..1183
                     /locus_tag="VFH91_02655"
     CDS             377..1183
                     /locus_tag="VFH91_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861910.1"
                     /translation="MKGSVFQLSLLCIVLLLVPTAQVEAQQPFYTDDADVTPRRHFHF
                     QFHNEFDVLARSAFPNLKQNTADFELDYGLFENVEIGIEAPFLTIINDHGELQRRVGG
                     VGDTNLSMKYNFLREKERSRLPAMAITFNLELPTGSVSRQLGSGLADFYLNGVLQKSL
                     TSRTTLRLNGGILFSGNETTGVVGIKARGTVFTGGASVVRQFTKRLDLGVELVGAETT
                     TFSLGKGQLQTQVGGNFAFLNNATLDFGIVAGKYVASPRIGAQLGVSIEF"
     gene            1273..1635
                     /locus_tag="VFH91_02660"
     CDS             1273..1635
                     /locus_tag="VFH91_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861911.1"
                     /translation="MQRDPATTARLMALLKEILVLTKSGQIHWQRQAGSAHLYATWHN
                     NLLIIGPTDPAEESNIPRYLFVTPLDSPAHVEINSNDEVLGPQLLELIQSVDDTTRGS
                     TPTDPFSLTDDVLNRLSH"
     gene            1684..2568
                     /locus_tag="VFH91_02665"
     CDS             1684..2568
                     /locus_tag="VFH91_02665"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET6861912.1"
                     /translation="MPTDESSLSHEAESLKRVALFKRLDAEELENLAAAVDQVNFKSG
                     EIIFNESDRGDALYVIDSGSVRIWVLDEDIKPVTLAELKEGDFFGELAVLDRGPRSTN
                     ATAITDTELHRLSSDDFQAFLLQHPETSIDVICEIGARMRQTNTLVTNRVTRNINVQM
                     ELTATLGDRVADKVASFGGSWTFIFIYCTFLLIWIATNSFVLYRYGHGENGAQFDPYP
                     YILLNLMLSMTAALQAPIIMMSQNRAAAKDRLAAEQDFKVNLKSELMLEELMRKSSAR
                     DAQIQEMADTVKSLSPPR"
     gene            complement(2580..3863)
                     /locus_tag="VFH91_02670"
     CDS             complement(2580..3863)
                     /locus_tag="VFH91_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6861913.1"
                     /translation="MKVIAMRRWLLDNKQAKRRATVPLIMGLFVGALFFGSSLAHSQG
                     GDSRPRNVQASPTPTPAVPASQPVQTASPTPAPQKQGVDRIAPSLGEPPPPPRLKPTP
                     SPTPPPEIAEGDTLKINADLVQLHVRVIDRNNRPINNVPQSQFHVFEDGVPQPIESFT
                     REEVPISYGLAVDTSGSLRSQLQSVIDAGKSIINSNKIGDETFLVRFVSSDNIQTVQD
                     FTANKEMLMDGLDNFYIEGGQTAVIDAVYLSAEHVSQYKKGDEGDRSRRALIVITDGE
                     DRNSFYKQDQLFERLREEDVQIFVIGFVNELDKDSGLIRKSPKERAVGLINRLASETG
                     GRAFFPDSISELPQIANEIIRDLRTQYVIAYNPTNKTPDGTFRAIKVTIDQPSNAEKR
                     IALSRTGRLARRETAGAPKPPPVKMPTSASPVKKP"
BASE COUNT          967 a          993 c         1063 g         1046 t
ORIGIN      
        1 gctgcagatg caatggttaa agctgccaac gttcgtctcg ttggttggga aaagatcgac
       61 gctggtttgg tcacagcgat cgttcgcggc gatgtcgcag ccgttcgcag cgcagtcgac
      121 gcaggcgcgg ccgcgggtaa acgagtaggt gaagttgtgg cgactcatgt gatcccccaa
      181 ccacactcgg acgtggacca ggggcttcct gtgcttcata ctggtgaaac aaccaagaaa
      241 aagatcagcg gctcggtaag agggaagagt tagttggttt ggagtgcggt ctgctgtcac
      301 caattagaat gcgagttccc ccaggttgat gaattaaagc ggtgataggt cgccgtactc
      361 caaaagttcc ccctccatga aaggatcagt attccaactc tcgttgcttt gcatcgttct
      421 cctgctggtt ccgaccgctc aagttgaagc acagcaaccg ttttacaccg acgatgcaga
      481 cgtaacaccg cgcagacact tccattttca attccacaat gagtttgatg tattagcccg
      541 atcagctttt cctaatctga aacaaaatac tgccgacttc gaacttgact atggtttgtt
      601 tgaaaatgtc gagattggga tcgaagcgcc gtttctgacg attattaacg atcatggcga
      661 gcttcagcgt cgagtcggcg gagttggcga cacgaatctc tcaatgaagt ataacttcct
      721 tcgggagaaa gaacgttcga gactcccggc gatggcaatc acttttaatc tggaattgcc
      781 cactgggtct gtgtctcggc aacttggatc tggccttgca gacttttacc tcaacggagt
      841 tctacaaaag tcgttgacct caaggaccac tctgcggctg aatggcggta ttttattctc
      901 cggaaacgaa acgacgggtg tggttggtat taaagcgaga ggaaccgtat tcacgggtgg
      961 agcgtctgta gtgcgtcaat ttacaaagcg gcttgacctg ggcgttgaac tcgttggagc
     1021 cgagactacg accttttcac tcggtaaggg ccaacttcag actcaggttg gtggcaactt
     1081 cgcatttctc aacaacgcaa cgctggattt cggcatagtg gctggaaaat atgtcgctag
     1141 cccgcgcatc ggagcgcaac ttggagtctc cattgaattc taagatggcc cgcgtgtaga
     1201 tgtcccacgt ggttctagaa tgttaggtta gttaatttat taggtcattg atcatataag
     1261 gaaggagtct caatgcaacg tgatccagca accacagctc gcctgatggc gctgcttaaa
     1321 gaaatcctgg ttctcacgaa aagtgggcaa attcactggc agagacaggc gggatctgct
     1381 cacctctatg ccacctggca taataacctc ctgataattg gcccaactga tccggctgaa
     1441 gagtcaaata taccgcgcta tcttttcgta actccgctcg actctccggc ccatgttgag
     1501 attaactcga atgacgaggt gcttggtccg cagctgctgg aattgatcca gtctgttgac
     1561 gatactactc gtggctcaac tcctacggat ccgttttctt taacggacga cgtgctgaat
     1621 cgtttaagcc attaacccgt tatcaggtct atcttctaca cgaaagttga gaagtgaaaa
     1681 gacatgccta ctgatgaatc atcactcagc catgaagctg aaagtcttaa gcgagttgct
     1741 ttattcaaac gcctggatgc cgaagagctg gaaaatcttg ctgcggcggt cgatcaggtc
     1801 aacttcaaat caggcgagat aattttcaat gagagcgatc gaggtgatgc cctctatgtg
     1861 attgactcag gctctgtgcg catctgggta ttggacgagg acattaagcc cgtgacgctt
     1921 gcagagttga aagaaggaga tttctttggc gagcttgcgg tcctggaccg tggcccacgc
     1981 tctaccaacg caaccgcaat tacagatact gaattgcatc gacttagcag cgacgacttt
     2041 caagcattct tgctgcaaca tcccgaaacg tccatcgacg ttatttgcga gattggggcc
     2101 cgaatgcgcc agactaatac gctggtcact aaccgggtta caagaaatat caacgttcag
     2161 atggagttaa cggccaccct gggtgatcgc gttgcagaca aggtcgcatc gtttggtggt
     2221 tcatggacct tcatttttat ctactgtact ttcttactaa tctggatagc cacaaactcg
     2281 ttcgtgctat atcgctatgg tcacggagag aatggtgctc agtttgatcc ttacccgtat
     2341 attcttctta acttgatgct atcaatgacc gcggcactcc aggcgccgat aatcatgatg
     2401 tcgcagaatc gggcagcagc gaaagatcga ctcgcagcag agcaggattt caaagttaat
     2461 ctgaagagtg aactgatgct tgaagagttg atgaggaaaa gcagtgcgcg ggacgcccag
     2521 attcaagaga tggctgacac cgtaaagtcg ctttcgccac ctcgatagct tgtccggatc
     2581 taaggctttt taactggtga agccgaagtc ggcattttta ccgggggcgg tttgggagca
     2641 ccagccgtct cccgtctggc caggcgacct gttctggaaa gcgcaattcg tttctcagcg
     2701 ttggaaggtt ggtcgatggt gaccttaatg gctcgaaacg ttccgtctgg cgtcttattc
     2761 gtcgggttgt aggcgattac atactgggtt ctcagatcgc gaatgatctc atttgctatt
     2821 tgtggcagtt ctgagatcga atctggaaag aacgctctgc cgccggtttc gcttgccagt
     2881 ctgttgatga gaccgacagc tcgctctttg ggactcttcc gaataagacc ggaatcttta
     2941 tcgagttcat tgacaaaccc aataacgaag atctgtacgt cctcttctcg taaacgctca
     3001 aacagctgat cctgtttgta gaagctattt ctatcttctc catccgtgat gacgatcagt
     3061 gcacgccggc tccgatcgcc ctcatcacct tttttatact gggacacatg ttcggcagac
     3121 agatacacgg catctatcac agctgtttgg ccgccttcga tatagaagtt atcgaggcca
     3181 tccatcaaca tttctttgtt cgctgtgaag tcctggactg tttgtatgtt gtcgctgctg
     3241 acgaagcgga ccaggaacgt ctcatcgccg atcttgttgc tgttgatgat gctttttccg
     3301 gcgtcaatta cgctttgcag ttgtgagcgc aacgaacccg aagtgtcaac ggctaagcca
     3361 taactgattg gtacttcctc tcgagtaaag ctttcgatcg gttgtggaac tccgtcttca
     3421 aaaacgtgaa attgactttg tgggacgttg ttgatcggcc gattgttgcg gtcgattacg
     3481 cgcacatgaa gctgaaccag atcggcgttg atcttcagag tgtcgccctc cgctatttcg
     3541 ggcggtggag ttggcgatgg cgtcggtttc agacgcggcg gcggtggcgg ttcgccaagc
     3601 gatggagcaa ttcgatctac tccttgtttt tggggcgctg gagttggact tgcagtttga
     3661 actggctggg atgcaggcac agctggcgtt ggagtcggag aagcttgaac atttcgtgga
     3721 cgcgagtcgc cgccttgact atgtgcgagc gaagaaccaa aaaacagcgc gccaacaaac
     3781 agtcccataa taagaggaac cgtcgcacgt ctcttagctt gtttgttgtc cagtaaccaa
     3841 cgcctcatcg caatcacctt catcaagttc ttaaattaaa ctcgagcgtg gaaaaaaggg
     3901 taaagaatat ctaaccgtgc cgctgtcctc aaatccagcc cgctcatacg aaacgccccg
     3961 tgagccctga ttgggatccg gagcgctgtt ggaagactaa ctggctaccg cgtgttgcac
     4021 gcgtgctggg gcaaacagag tcgcaagccc aagcggcgac cgagcgcac
//
LOCUS       DASSFP010000126        11051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1358548, whole genome shotgun sequence.
ACCESSION   DASSFP010000126 DASSFP010000000
VERSION     DASSFP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358548"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2862)
                     /locus_tag="VFH91_02675"
     CDS             complement(<1..2862)
                     /locus_tag="VFH91_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861914.1"
                     /translation="MTAQSGSYRFFRWLPLFVLLFAISASAQTPSRARLPESSIKTVY
                     IVPTSHYDFGFVEPPDQVRERAARHIDEVLRMAESDPDFRWTIESVWQINEWLKRQKP
                     ATSVLPKDNAKIARLMSLIKSGRIALSTAWGSMHTDFMGAEELNRLCYDYTVLRRTYG
                     VESQLALMDDVPGHPTSIPSVLANSGTKYLVTGLNLFLGPATDLAPGKVPFYWQAPDG
                     GKVLIWISQGKRGGYVEGMTDFYLDPYSLDPYTNKTPYEMFNPNAGPKTDLQKMEEGV
                     TELLNRYNGGGYKYDSVMVMYAHDFVEPTNVKNLEKAVALWNSNHPEIQLKIATPPEF
                     FRAIESKYQAQIPTYRGEFSGLWSEAKTQSPVLSNLARFAHEQTPAAESLWSALSMTR
                     GIPFPVGNMSSLYDWMFTYDEHSGAGNTGWIQLNDRGLLEEQNRQYVRFMKNARAEVE
                     NLFSRGLSLAAQSTRYDQPFKQTSANEWNLLVYNGLSWSRSDLVQISSPREGQKVVEI
                     NDASTKQPVLFDVDDNGQVFFLARDVPSLGYKTFTVTTAPGKSTTTLTTLPRSVDTSN
                     SNYRVKLRPDGNIQSIYDVRSSREIVNDKGELPFNELLRVEGANAARLAAPSNPVITI
                     RKGKLLTEISIERSRSAFYSTVIRIYEGLERAEIANSIDGSRLPFPGGSSNWSDNYYF
                     SFPFSVSKDNLKIMRGGQKWFDTLPDDYIPGARKDSVTTQHLIGLTDGNATAMLAHRQ
                     AFHFAYAGFVNTKVLPKGAPQEFPAMYTGKFPLPEATVYSHAFRHTEQADTHDLGVVN
                     MATVEPGLGDQYLFEYAVRAGGKWDSVTAWHFGAEFNLPLRAAYIDVPPSQPVRSFFD
                     IDQPNVQIVSIKPLSDTVVHGEVSATPLNPQLNRRYSIRLQEFTGRATEVRINLPVQI
                     KSATRMNLTEDVELEKIAGISPLTVRLEPFSTATILIEIE"
     gene            complement(2900..5941)
                     /locus_tag="VFH91_02680"
     CDS             complement(2900..5941)
                     /locus_tag="VFH91_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6861915.1"
                     /translation="MKKQLCRIIKQVSVLIVAVCVFAVVGLAQSNTGSIVGVVQDPNG
                     SPIPGATVMVTNVGTNDNKTVQANAEGYYEALSLPTGVYRIVGKASGFEDATLSDVRL
                     AVGDRLRMDLQMGVGAVSANVMVAAQTNVETETSTLGDTITSARVENVPINGRDFTGL
                     LSTVPGSVQTSNQFQTSINGIPSTFGGSSVLVDGIDAGRVDLNGTSNVLGRIESRVNR
                     VSMDSVQEVQVLEQNYSAQYGQAIGTVINPITKSGTNNFHGSVFDYFRNEALDANDFF
                     NNEAGFPRNEFRLNQFGGNVSGPIVKDKLFFFGNYEGVRQTRGSLFRGFTPTASFRTE
                     FVPSLNAALATLPLPNTNNFLNVDADPAFEYGEFRVLKNGKLREDTGSVKLDYQYNDR
                     SQFSFRYNLNDSNTTTPYGVGTDQTADGTLRVQLLKLSHNYTLSSSSVNEFAFGINRN
                     VTNPGAGPSDLPIFNFLFADSSIAGPGPAQFNQFRTNTVYQFLDTFSTLKGNHSLKLG
                     TDIRLNRRDAESVTQFTLFFFSGADFANAAALLGQSSGNPKLSYANENYSFFVQDDWK
                     VHPRLTLNLGLRYDISSVSREAEGRLQNFILATKSFTPLGQKIYNADKNNFGPRFGFA
                     WDIFGTQKTVLRGGYGLFYDRDLPASFGSPQINTFPSVQIFFPSFPINPAEFNTAGLD
                     GRQVLMINPDLPTTYAQHWSLNVQQDVRIGILQVGYVGNHVLDILTNGVVTPRNINPN
                     LDAFGTKPIPGVGNILDIGTYPQSNYNALQATFKRNLTKGLRINANYTWSHEIDNVIG
                     FFKDYQNYQDLNADRASGDQDIRHNFTFDAGYDVPSLREFFGDGTPRWIADGWQLNTL
                     TQMRTGFPVNVTVTGGIFGGALRPNLIEGVPLRPANYDLPKNQFNLAAFALPAPGTFG
                     NLGRNALRGPGFAQVDFSVSKETKITETQSFQLRFEFFNLFNHANFADPSGGINADSF
                     GGSLNRTALFGQSVFTVGNQLGGLLGPGGPRQIQISARYNF"
     gene            complement(5964..7001)
                     /locus_tag="VFH91_02685"
     CDS             complement(5964..7001)
                     /locus_tag="VFH91_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6861916.1"
                     /translation="MNNPAKVKTEPKTAVYTPPPFLDEAIAAVPLVSAATRPGRIGYL
                     TNYSFHIWYQIVIEVLGRRAAQYGSEIVVQDAGKSVENQINQAREMLPKVDALLLTPA
                     ATSGLEPVLQLADAAGKPVIIEANPVEGMTTLVAICDYDAGYDLGVWIGENVTPEKGA
                     LRILDVGLPWLRPCLLRSEGFVDGVRSVQSDAIVVASINGEAVPEVAHRVALDKLRET
                     GDVDVIFAMDDETGQGAYEGYVEAGFNPDNVVIAGFGLAGDHEKEWLMRREALKVSAA
                     MFPEYVALRCMDNLIKGYDGQKTPLRDVIPTVAATPELLEKLYPKKGNAWTPDFAAIG
                     ALSGKSECTRV"
     gene            complement(7022..7807)
                     /locus_tag="VFH91_02690"
     CDS             complement(7022..7807)
                     /locus_tag="VFH91_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6861917.1"
                     /translation="MKFERKNVVVTGGAQGIGAATSALLAERGASVAIFDWDEVNGIK
                     TVREIEESGGKADFSRVNVADYDSVERAVARVVEIWGSVNSLVVSAGIQRYGTALTTS
                     TEEWNEVLGVNLQGAWNAARAVIPFLKRAGGGTIVNVSSVQALATQPNVLAYTVSKHA
                     LIGLTRSIAIDFANQNIRVNAVCPGSVDTPMLRWAASLDRNPESVLEACNQMHPLGRI
                     AQPREVAEVIAFLAHESSSFVTGAVWTVDGGLMAQLGGAPRAA"
     gene            complement(7797..9668)
                     /locus_tag="VFH91_02695"
     CDS             complement(7797..9668)
                     /locus_tag="VFH91_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861918.1"
                     /translation="MNLSRIDQLIVVLYLAAIMGIGIAMKRKAARGMSSYFLGGRQLP
                     WWALAMSGSSSYFDITGTMWIVSTFIALGLKGMWVHWLWGFPITAFYLAYMGKWIRRS
                     NVMTGAEWMKTRFSSGKAGDIARLSYTLFAILTITALLGYSAIGMGKFGAIFLPFSPT
                     VCAVLILGITGIYVVAGGLHGIVRVEIAQTVVLSTGAIVFAVVGYRHFNPAHFATLVP
                     ASWMDILPSMRPADLQGVVSGGTDYSLFGALVAVWVLKGFLLCLSGPEQLYDFQRFLA
                     ARDERDASKLGALWGVIHTVRWCFAMAIAAMAISGIGNAVLDARLKADPETALPLIIG
                     TMLPVGLVGFMLAALLSGFLATFSSTVNGGAAYLVKDVYQKYINPEASPKTLVRASYV
                     SSVLLIIAGLVISVFGTSINTAFLWIFGTLAAGILPPNVLRWYWWRLNGYGYAAGVFS
                     GMFLSLGQVVLDTTYLSQPLPLYIGFPVIALASTIITIVVALLTQPTDIQTLKNFYRN
                     VQPAGAWGPAREALLAEDPGFKKQSPFSRDALNTIVAMIGITSLYVSMLYLVLHRHVV
                     AFALLSTAALTAIVLYFTWYKHLPAPQSLSSEEFDPDRAEVSFNSAERVVVGGSREV"
     gene            complement(9945..10736)
                     /locus_tag="VFH91_02700"
     CDS             complement(9945..10736)
                     /locus_tag="VFH91_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6861919.1"
                     /translation="MRRIISKIASNSLQREAPYSVLLPSAYDSLDLSYPILYLLHGLF
                     GSHENWVELTNIEELSSNLHLIIVMPDGSDGWYCDSETISANRYESFLIEELIPQIER
                     KFRTIPKREARAIAGLSMGGYGAFKFSVLRPDLYQFAASFSGAFDVPERSDDAPGFDW
                     NTLRPSVLQAFGAPGSNTRTRNDLYRLFQSLKAEDINALPYFYFNCGRDDGFMEANVR
                     LNSLFDAFGISHQFAEIEGGHDWDYWGSRLPDLLKMTTERLVGPR"
BASE COUNT         2808 a         2923 c         2697 g         2623 t
ORIGIN      
        1 ctcaatctcg atgagaatag tcgcggtaga gaacggctca agccgcaccg tcaagggcga
       61 gataccggca atcttttcca attcaacgtc ttccgtcaga ttcatgcgag tcgcagattt
      121 aatttggaca ggaagattaa tccgcacctc ggtcgcgcga ccggtgaact cttggagtcg
      181 gattgagtat cgcctgttca attgaggatt taaaggtgtt gcgcttacct cgccgtgaac
      241 cactgtgtct gacagaggtt tgatcgaaac aatctgtacg tttggttgat cgatatcgaa
      301 gaagctccga acaggttggg acggaggcac gtcgatgtaa gcggcacgaa gaggcaagtt
      361 gaactctgca ccgaaatgcc aggccgttac tgaatcccat ttcccgcctg ctctgaccgc
      421 atattcgaat agatactgat cgccgagacc cggctcgacc gtcgccatgt ttacgacgcc
      481 gagatcatga gtatcagctt gctcagtatg tcgaaaggca tgtgagtaaa cggtcgcttc
      541 gggaagcgga aacttgcctg tgtacatcgc gggaaactcc tggggagcac ccttcggtag
      601 tacctttgtg ttgacgaatc cagcgtaagc aaaatgaaaa gcttgccggt gggccaacat
      661 tgccgtggca tttccatcag taaggccaat taaatgttgg gtagtgacag aatcttttcg
      721 agccccggga atgtaatcat cgggcaaagt atcaaaccac ttctgtccgc cacgcatgat
      781 cttcaaattg tccttcgaaa ccgagaacgg aaacgagaaa tagtagttat cactccaatt
      841 gctgcttcca cccggaaagg gcagccgcga accatcgatt gaatttgcaa tttccgctcg
      901 ttcaaggcct tcgtagattc tgatcacagt cgaataaaaa gccgatcgcg agcgttcgat
      961 tgagatctca gtgagcagtt tgcctttcct aatggttatc accgggttgc tcggcgccgc
     1021 cagccgtgca gcattggcgc cttccactct caagagttcg ttgaacggca actcaccttt
     1081 gtcattaaca atttcacgac ttgatctgac gtcataaatg ctctgtatat ttccatcagg
     1141 tcgaagcttc acccggtaat tggagttgct tgtgtcgact gagcgcggga gcgtagttag
     1201 tgttgttgtg gattttccgg gagcggtggt tacagtgaac gttttgtaac ctaaggatgg
     1261 aacatctcta gccaggaaaa atacttgtcc gttatcatca acatcgaaaa gcaccggttg
     1321 ttttgtcgaa gcgtcattta tctcgacaac cttctggcct tcgcgcggcg agctgatctg
     1381 gaccaggtca cttctggacc atgacagacc gttgtaaacc aacaaattcc attcatttgc
     1441 tgaagtttgt ttgaatggct gatcgtacct tgtcgattgt gccgcgagcg ataatccgcg
     1501 cgagaacaga ttctcaactt ctgcccgggc atttttcatg aaccttacat attgacgatt
     1561 ttgttcttcc agcagtcctc gatcgttcaa ctgaatccag ccggtattcc ccgcgccgct
     1621 atgttcgtca taagtgaaca tccaatcgta aagagacgac atgtttccca ccggaaatgg
     1681 gataccgcgc gtcatcgaaa gagcgctcca caaactctca gccgcaggtg tttgttcgtg
     1741 cgcaaaccgt gcgagattgc tgagcacggg agattgggtc ttggcctcag accataaacc
     1801 tgaaaattca cctcgatatg taggaatttg agcctggtac ttgctctcga tggcccggaa
     1861 aaattcaggt ggagtagcga tcttcagctg tatctccgga tgattgctgt tccagagcgc
     1921 gactgccttt tccagattct ttacgttcgt tggttcaaca aagtcatgag cgtacatcac
     1981 catgactgag tcgtacttgt aaccaccacc gttatagcga ttgaggagtt ctgttacgcc
     2041 ttcctccatc ttttgcagat ccgtctttgg accagcattt ggattgaaca tttcgtaagg
     2101 cgttttattt gtgtaagggt caagcgaata tggatcgagg tagaaatcgg tcataccctc
     2161 aacataaccg cctcgcttac cctgactgat ccagataagg accttccctc catcaggagc
     2221 ctgccagtag aaaggaacct tgcctggcgc aaggtccgtt gctggaccca gaaacagatt
     2281 tagaccagtc accagatact ttgtgccact gttggctaac actgacggta tagaagttgg
     2341 atgaccggga acgtcgtcca taagtgcaag ctgcgactcg actccatacg tacgacgcaa
     2401 aactgtgtag tcgtaacaga gccggttcaa ctcctcagct cccatgaaat ccgtatgcat
     2461 gctgccccat gcagtcgaaa gagcaatgcg ccctgatttt attaaggaca tgagacgcgc
     2521 gatcttcgcg ttgtcttttg gcaaaacgga ggtagcgggc ttctgtcttt tcagccattc
     2581 gttgatttgc caaacactct cgatggtcca acgaaagtca gggtcagact ccgccatccg
     2641 caggacctcg tctatatgcc tcgccgcgcg ttcgcgtact tgatcgggag gctcgacaaa
     2701 gccaaagtcg tagtgacttg tgggaacgat gtagacagtc ttgatcgagc tctcaggtag
     2761 ccgcgccctg gaaggtgtct gggcgctcgc tgaaatcgcg aataacaaaa caaaaagcgg
     2821 caaccaccga aaaaaccggt agctgccgct ttgcgctgtc aattttttgc cagccagaag
     2881 cattcaattg gtgtccttgt cagaagttgt atctagcgga gatctggatc tgacgcggtc
     2941 cacctggacc aagtagacca ccaagttgat tgcccacggt aaatacgctt tggccgaaca
     3001 aagccgttcg attcagactg ccgccgaacg aatcggcatt gatgcctccc gaaggatcgg
     3061 cgaagttagc gtgattgaac aggttgaaga actcgaaacg gagctggaag ctttgcgttt
     3121 cggttatctt cgtctctttt gataccgaga agtcgacctg cgcgaagcct ggaccgcgca
     3181 gagcgttgcg tccgagattt ccgaacgttc cgggagccgg caacgcaaag gccgcgagat
     3241 tgaattgatt cttcggcaga tcgtaattag ctggtcgcag cggaacaccc tcgatcaagt
     3301 taggccttaa cgctccgcca aaaatacccc cagtcacagt gacgttgacc ggaaaaccag
     3361 tacgcatctg cgtcaaggtg ttgagctgcc aaccatcagc aatccagcgc ggagttccgt
     3421 caccgaaaaa ctcacgcaac gaaggaacat cgtaaccagc atcgaacgtg aagttgtgac
     3481 ggatatcctg atcgccactg gcccgatcag cattcaagtc ctgatagttt tgatagtcct
     3541 tgaagaaacc gatcacatta tcaatctcgt gcgaccaggt gtagttcgcg tttatgcgca
     3601 acccttttgt caaattccgc ttgaatgtgg cctgaagtgc attgtagttg gactgcggat
     3661 aggttccgat gtccagaata ttgccaaccc caggaatcgg tttcgtacca aacgcgtcga
     3721 gatttggatt gatattacgc ggagtgacca ctccatttgt cagaatgtcg agcacatggt
     3781 tcccaacata acctacttga aggataccga tgcgaacatc ctgctgaaca ttcaacgacc
     3841 agtgttgagc ataggtcgtc ggcagatcag gattgatcat caaaacctgc cggccatcga
     3901 gaccagctgt attgaactcg gcgggattaa tcggaaaaga cgggaaaaag atttgtaccg
     3961 acgggaacgt attgatctgc ggcgagccaa aacttgcagg caagtcgcgg tcataaaaca
     4021 gtccataacc gccgcgcagc accgtcttct gggtgccaaa aatatcccac gcaaaaccaa
     4081 atcgtgggcc aaagttattc ttatcggcgt tgtaaatctt ctgccccagc ggggtaaagc
     4141 tcttagtcgc cagaatgaag ttttgcaaac ggccttcagc ttcacgactc acactgctga
     4201 tgtcgtatcg caatccgaga ttcagcgtta gacgcggatg aactttccag tcgtcttgca
     4261 caaagaacga atagttctcg ttggcgtagc ttagcttcgg gttgccggag ctctgtccga
     4321 gcagtgccgc cgcgttcgcg aaatccgctc cactaaaaaa gaacagtgtg aactgcgtta
     4381 cggattcagc gtcgcgacga ttgagcctga tgtccgttcc aagctttaac gaatgattac
     4441 ctttcaaagt gctaaaggta tccagaaact gatagaccgt gttggtacgg aactgattga
     4501 attgagccgg ccctggaccg gcaattgatg agtcagcaaa aagaaagttg aagattggca
     4561 ggtcagatgg accagcacca gggttggtaa cgttccgatt gataccaaaa gcaaactcgt
     4621 ttaccgacga actcgataag gtgtagttgt gcgagagctt taggagttga actcgcagcg
     4681 ttccatcagc agtctgatct gtgcctacac cgtacggtgt cgtcgtattt gagtcgttca
     4741 ggttgtaacg aaacgaaaac tgactacggt cgttatattg atagtcaagc ttgaccgagc
     4801 ccgtgtcctc tcgcaacttg ccattcttca atacgcgaaa ctcaccatat tcaaaagcag
     4861 gatcggcatc gacgttcagg aagttgttag tgttgggaag cggtaatgtc gcaagagccg
     4921 cattgagcga aggtacgaat tcagttcgga acgatgcggt tggagtaaag cctcggaaca
     4981 aactgccacg agtttgtctg accccttcgt agtttccaaa gaagaagagc ttgtccttta
     5041 caattggacc gctgacgttc ccgccaaatt ggttcagccg aaactcattt cgaggaaaac
     5101 ccgcttcgtt attgaagaag tcatttgcgt cgagcgcttc gttgcggaag taatcgaata
     5161 cacttccatg gaagttgttg gtaccggatt tggtaatcgg attgataact gtgccgattg
     5221 cctgcccgta ctgggccgaa taattctgct cgagtacctg aacctcctgc acactatcca
     5281 tacttacgcg gttgacgcgc gactcgattc taccgaggac gttggaggtt ccattcaagt
     5341 caacgcgccc ggcatcaatt ccatccacga ggacactact gccaccgaac gtcgaaggaa
     5401 taccgttgat gctggtctga aactgattcg acgtttgtac cgatccgggt acggttgaga
     5461 gcaacccggt gaagtcccga ccgtttatcg gtacattttc gacccttgcg gaagtaatcg
     5521 tatcgcccag ggttgacgtt tccgtttcga cgttagtctg cgccgccacc atgacgtttg
     5581 cgctgacagc gccaactccc atctgaagat ccatgcgtaa cctgtcacca accgcgagtc
     5641 ggacatcgct caaagtagca tcttcaaaac ccgacgcctt gccaacgatt ctgtaaactc
     5701 cagttggcag tgaaagcgct tcgtaatagc cttcagcatt cgcctggacc gttttgttgt
     5761 catttgtacc aacattagtc accatgaccg tggcgccggg aatcgggctt ccattcgggt
     5821 cctgcacgac tccaacgatc gaacctgtgt tcgattgcgc gagaccgacg accgcaaaga
     5881 cgcacacagc gacgatcagg acacttactt gtttaatgat tctgcacaac tgttttttca
     5941 tctcacgcct ccaaagtttc ttcctaaact cgcgtacatt cactcttacc ggaaagagcg
     6001 cctattgcag cgaagtcagg agtccaggca tttccttttt tgggataaag tttctcaagc
     6061 agctcgggag tagccgcaac tgtgggaatg acatctcgaa gtggtgtttt ttgaccgtcg
     6121 taccccttaa tgagattgtc catacaacga agggcaacat attctggaaa catcgccgcg
     6181 ctaactttta gggcctcgcg acgcatcaac cactcctttt catgatcacc tgccaacccg
     6241 aatcctgcga tcacaacgtt gtcaggatta aagccggcct cgacgtaacc ttcgtaagca
     6301 ccctggccag tctcgtcgtc catggcaaag atcacgtcaa cgtctccagt ttcacgcaac
     6361 ttatcgagcg cgactcgatg cgcgacttcg ggaacggcct cgccattaat gctcgctaca
     6421 actatcgcgt ctgattgaac cgacctgacg ccatcgacaa aaccctcgct gcgcagtaag
     6481 cacgggcgga gccacggcag tccgacatcg agaatccgta acgcgccttt ttccggcgtc
     6541 acgttctcgc ctatccaaac accaaggtca taaccagcgt cgtaatcgca gatcgcgacc
     6601 agcgttgtca tgccctcaac aggatttgct tctatgatga ccggttttcc tgcggcgtcc
     6661 gccaattgca ataccggttc aagtccagac gttgcggctg gcgtcaacaa caacgcgtca
     6721 acctttggca gcatctcgcg cgcttgatta atctgattct caacactctt accggcatcc
     6781 tggaccacaa tttctgatcc atattgagca gcccttcgcc ctaatacctc gatgacaatt
     6841 tgataccaga tgtgaaagct gtagtttgtc aggtagccaa tgcgcccagg cctcgtggca
     6901 gccgatacca gtggtaccgc tgcgattgct tcatcgagaa atggaggcgg tgtatagacc
     6961 gccgtcttcg gctcagtttt aacctttgct ggattgttca ttactactcc ggtgtttctt
     7021 actacgctgc tctgggcgct ccgcccagtt gggccatcag ccctccgtct acggtccaaa
     7081 ccgccccggt aacgaatgac gaactttcat gggccaggaa tgcaattacc tcagcaacct
     7141 ctcgcggttg agcaatgcga cccagcggat gcatttgatt acaagcttcc aaaaccgatt
     7201 caggatttcg atcgagagat gccgcccaac gcagcatcgg cgtatcaaca ctgcccgggc
     7261 aaaccgcatt aacgcggatg ttctgatttg caaagtcgat cgcgatcgaa cgagttagac
     7321 caatcagcgc atgcttactg actgtgtaag caagcacatt gggctgggtg gctaacgcct
     7381 ggaccgacga aacattcaca atggttccgc cccccgctct ttttaagaaa ggaattaccg
     7441 cacgcgccgc gttccatgcg ccttgaaggt ttacgccaag cacttcgttc cactcctcgg
     7501 tgctggtcgt aagtgccgtc ccataacgtt gtatccccgc acttacgacc agcgagttaa
     7561 cgctccccca gatttcgaca acacgcgcga ccgcacgttc aacgctgtcg taatccgcta
     7621 cgttaaccct tgaaaagtca gctttgccgc ccgactcctc aatctctctg actgtcttaa
     7681 tcccgttgac ttcatcccag tcgaaaattg caaccgacgc tcctcgctca gcgagcaagg
     7741 cggaagtcgc agctccaata ccctgagcgc ctccggtaac aactacattc ttcctctcaa
     7801 acttcacgcg accctccgac cactacacgt tctgcagaat tgaaactgac ttcggcacgg
     7861 tcgggatcaa attcttccga cgacaaactc tgaggcgcgg ggagatgctt gtaccaggtg
     7921 aaatagagaa cgatcgcggt aagtgctgca gtggaaagca gtgcaaaagc tacaacgtgt
     7981 cgatgcagga ccaggtaaag catcgacacg taaagcgacg tgatgccgat catcgcaacg
     8041 atggtattca acgcgtcgcg tgagaaaggc gattgttttt taaacccggg atcttctgcc
     8101 aggagtgctt cgcgagctgg tccccaggca ccggcaggct gcacatttcg gtaaaaattc
     8161 ttgagtgttt gaatgtcagt aggctgagtt agcagcgcca caacaatcgt gatgattgtt
     8221 gaagcaagcg cgataaccgg aaaaccgatg tacagaggca gaggctgact cagatatgta
     8281 gtatcaagga ccacctggcc caacgataaa aacatgccgc taaaaacgcc tgccgcatac
     8341 ccgtaaccgt tgagtctcca ccagtaccaa cgaagcacgt tgggaggaag aatgccggct
     8401 gctagagttc caaaaatcca gaggaaggcg gtatttatag aagttccgaa tactgagatg
     8461 accagaccgg caatgattaa aagcaccgac gagacgtaac tcgcgcggac caacgttttc
     8521 ggactcgctt ccggattgat gtacttctgg taaacatcct tcaccaggta ggcagcgcca
     8581 ccattgaccg tagaactaaa ggtcgcgaga aagccagaca gaagcgccgc caacataaag
     8641 ccaaccaatc cgaccggtag catggtgccg atgatcaatg gcagcgcagt ctcaggatcg
     8701 gccttaagtc tcgcatcgag aacggcgtta ccgattccgg atatcgccat tgccgcgatg
     8761 gccatggcaa aacaccaacg caccgtgtgt atgactcccc agagcgcgcc taacttcgac
     8821 gcatcgcgtt cgtcgcgagc ggcgagaaat cgctggaagt cgtacagctg ttcaggacca
     8881 ctaagacata aaaggaatcc cttcaaaacc caaactgcca cgagcgcgcc aaacaacgaa
     8941 tagtcagttc cacctgaaac gacaccttgc aaatccgcgg gtcgcatcga cggcaaaata
     9001 tccatccacg aagcaggcac cagggttgcg aagtgcgcag gattaaaatg acgataacct
     9061 acgaccgcga acacaatcgc gccggtgctc agtaccactg tctgagcgat ttcgactcgt
     9121 acgattccat gcagaccacc cgcaacaacg tagatgccgg tgatacccag gatcagcacg
     9181 gcacaaacgg tcggtgaaaa gggtagaaag attgccccga acttacccat tccgattgcg
     9241 ctgtaaccca gcaaggcagt aatcgtcagg atcgcgaata acgtgtacga gagtcgcgcg
     9301 atgtccccag ctttaccgct cgaaaaacgt gtcttcatcc actcggcgcc agtcattaca
     9361 tttgaacgcc gaatccactt tcccatgtac gcgagataaa acgcagtgat gggaaatccc
     9421 cagagccagt ggacccacat acctttgagc cctaaggcaa tgaaggtgga gacgatccac
     9481 attgttccgg tgatgtcgaa ataagacgac gagccagaca tggccagcgc ccaccagggt
     9541 aattgccggc cgccgaggaa ataggacgac attcccctcg cggctttgcg tttcatggcg
     9601 ataccaatcc ccatgattgc agcgaggtag aggacaacaa ttagttgatc tatccgagaa
     9661 aggttcatcc tgtttacggg tttgttccca ccgcgaacta ttaactgata gtcagggaga
     9721 gacgttcctg gttaaagcct ttactaacaa aactttagtt tgatttacag ctttctggcg
     9781 ttgcgcgggc cgctttatct tcgctgttga ttaagtcata actaacacag ccaacttatc
     9841 tcagtcaact attttttctg acgacccgat cttttctctg ttgcggaaac tcttgagcaa
     9901 cattttcaaa atcatcagag cgaaactgca acgtgtttag aaaatcagcg gggaccgaca
     9961 agccgttcgg tggtcatttt caatagatcg ggaagtctgc ttccccagta atcccaatcg
    10021 tgaccgcctt cgatttcagc gaactgatga ctgattccga acgcatcgaa gagcgagttg
    10081 agacgaacat ttgcctccat gaatccatcg tctcgtccgc aattgaagta gaagtaaggc
    10141 agagcattaa tgtcttcagc tttcaaggat tggaatagcc tgtaaagatc attgcgtgtt
    10201 ctggtgttgc tcccaggagc cccaaatgcc tgtaaaaccg acggtctcag cgtattccag
    10261 tcaaagccag gtgcatcatc cgagcgctct ggtacgtcaa aagctccact gaaagaagca
    10321 gcaaactggt atagatcggg acggagaacg gaaaatttga aagctccata ccctcccatc
    10381 gagaggcctg ctattgctcg ggcctctctt ttaggaatag ttcgaaattt tctttcgatc
    10441 tggggtatta attcttcaat caaaaaactt tcgtatcgat ttgcagaaat tgtttcgcta
    10501 tcgcagtacc aaccatcgct tccatccggc atgacaatta ttagatgcag attgctcgaa
    10561 agctcttcga tattggtcag ctcgacccaa ttctcgtggc ttccaaacaa cccgtggagc
    10621 agatacaaga ttggatagct gagatccaag gaatcgtacg cagacggaag aagcaccgaa
    10681 tatggcgcct cgcgctgaag gctgttactg gcgattttac taataattct ccgcattctc
    10741 gggattggcc agtggtggcc accgccctgt aatattcttt atcttgaccg gtgacaaagt
    10801 agcgagtgtt gtgtaacata cgaaacttca cggtgtcaaa aaccgagcag ttcgggaaag
    10861 cagaagttaa tccgtgtcac caggttattc gaattacgat ctccaaccac acactcaaat
    10921 agcctcaagt gtgcaatgtc accagggctg ggaagaaatt ctcggcgcca ttcatcgaaa
    10981 tgtctccagt ggtcatttca ttctctgtat cgagtgttat ccaggcgtgc cggtttctga
    11041 gataagtgac c
//
LOCUS       DASSFP010000127         5013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_475561, whole genome shotgun sequence.
ACCESSION   DASSFP010000127 DASSFP010000000
VERSION     DASSFP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475561"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFH91_02705"
     CDS             complement(<1..210)
                     /locus_tag="VFH91_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861920.1"
                     /translation="MKITLKRSIWLLLFLSIAAICISAFRSKDKGEADRMSRVPQTVV
                     WAWERPEDLKFIDPAKIGVAYLAKTI"
     gene            454..2163
                     /locus_tag="VFH91_02710"
     CDS             454..2163
                     /locus_tag="VFH91_02710"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HET6861921.1"
                     /translation="MERETLEFDVVFVGAGPANLSGAFHLKRLINEHNDAVANGKQDG
                     PPLGEVEIAVIEKGATVGAHILSGAVMDPRALRELIPDFVEQGAPLESPVNEDFFLYL
                     TAKHAVRSPITPPPLKNHGYYIVSLNRLTAWLGEKCEEVGVNIFPEFPGAELLFNDCD
                     RVLGVRTGDKGIDKEGKQKANYEPGVDLLAKVTVLGEGPRGSLTKKLIERLGLDEGKE
                     PQVYSIGVKELWELPDDRYPTGRVTHTLGFPSDSQTYGGGWIYGMQNRILNIGYVTGL
                     DYKDPMLDPHAEFQRYKTHPYIAKLLDGGKMIRYGAKTIAAGGYFAMPRTFADGVLIT
                     GDSAGLLNSQRLKGIHSAIKSGMLAAQSIFQALLAQDYSASQLKSYERGLQDSWIIPE
                     LRKVRNFHAGFKRGRWLGIVNAGLQFFTGGRAWGILDRDKAHAGHLEMKKLSAYGYRG
                     DRLEQRYDGLKFDGKLTFNKVTDVYHAAVGHDEDQPAHLHVLDTNICSTRCVEEFGNP
                     CQRFCPAAVYEMVDEGATGRKRLQINFSNCVHCKTCDIMDPYQIINWVTPEGGGGPDY
                     KGM"
     gene            complement(2170..2418)
                     /gene="rpoZ"
                     /locus_tag="VFH91_02715"
     CDS             complement(2170..2418)
                     /gene="rpoZ"
                     /locus_tag="VFH91_02715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET6861922.1"
                     /translation="MPTSTELVVPEIEGRETPEVDSKYRLIILAAKRSKQLQRGAQPR
                     IEIDPQKHKPTRIALEEVIRGRVHFTIKHDGHAQSPAE"
     gene            complement(2435..3118)
                     /gene="gmk"
                     /locus_tag="VFH91_02720"
     CDS             complement(2435..3118)
                     /gene="gmk"
                     /locus_tag="VFH91_02720"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET6861923.1"
                     /translation="MSSRVTEVVSQKTISGRAPRGTLFVVSSPSGGGKGSIIRRLLDV
                     VRVPNLSYSVSYTTRAPRNGEVEGREYFFVNRNTFGEMVAAGEFLEWANVHGNLYGTA
                     KRQVLDETANGVDIILEVDVQGAASVRKLLVDSISVFILPPSYEVLRDRLIARGTDSP
                     EELEVRLRNAPQELSQYTAFDYVILNDRLDRAAAQLASIIDAERARCVRQETLINAVI
                     EQFKLDDRK"
     gene            complement(3108..3986)
                     /locus_tag="VFH91_02725"
     CDS             complement(3108..3986)
                     /locus_tag="VFH91_02725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET6861924.1"
                     /translation="MKSMTGYGKGTVSADDFSVSVDLKTVNNRFLDIHLRVGTEWSSF
                     EPVIKRRISSRLSRGRVDIAVSVERTTQTAYELNRPLIAGYVAALRQMQQDFGIGGDI
                     DINVLARIPGALQPAKSGPDERIGVALEQALDLGLDELEKMRQQEGEALRLELRERVG
                     RIEGLVPTIESAAAGLADAYRQRLQKRIAELLNRGGQVVEIDPARLAQEVAYLADRSD
                     VSEEMVRLRSHLSQFQEALEAQGETGKMLDFLLQELNREANTTLSKSTDLTIKEAGLA
                     IKAEVEKLREQVQNVE"
     gene            complement(4016..>5013)
                     /locus_tag="VFH91_02730"
     CDS             complement(4016..>5013)
                     /locus_tag="VFH91_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6861925.1"
                     /translation="AVFLLFFGQREIIAGRMNTAQFLTYIFFLFRSYDPVRKLSRLQN
                     SLEQAFAAAHHVWEVMNEHSALKEKPDARELAPLQKKIVLDNVFFGYDNETRAVLRGV
                     NLNIPAGAMVALVGESGGGKSTLTKLLPRFHDPSSGSVLWDGTDLRDVTLSSLRSQIA
                     LVTQETVLFNDTVRHNIAYGKPEASDAEIEQAARIAFAHDFIKEMPDGYNTIVGERGT
                     FLSGGQRQRLAIARAILVNAPVLILDEATSALDAESERLVQRAISNLVRDRTTIVIAH
                     RLSTVRRANLIVVMKAGRIIEMGTHAELLSKNGQYRKLYELQFADEEELLAGVRE"
BASE COUNT         1176 a         1298 c         1297 g         1242 t
ORIGIN      
        1 aattgttttc gccaggtatg cgacccctat tttcgcgggg tcgataaact ttaaatcttc
       61 cggtcgctcc cacgcccaga cgacggtttg aggcacgcga gacattcgat ccgcctcccc
      121 tttatctttt gaccgaaacg cggaaataca aatcgcggcg atgctaagaa agagcagtaa
      181 ccaaatgctg cgtttcagcg tgattttcat ggcgatgcgg aagtgtacac caggcaagct
      241 gtctggaatg caacgaaatt ttgaaaacga aacgcctcag cactcgccgt ggaaattgat
      301 aaatctcaaa atgacaaatg accaataaac aaactcaaca cactataatg ttacatgctt
      361 cttcgccgtt tagcgccgta gcgtcgtttt gctatactcc aaactgaatg gccattcatt
      421 tttgtctact ctcgtctcag gaactcagaa taaatggaac gtgaaacctt agagtttgat
      481 gtggtattcg tcggcgctgg tcccgctaac ttgagcggcg cgttccacct caagcggctg
      541 atcaatgagc acaacgacgc tgtcgctaac ggtaagcagg atggaccacc gttgggtgaa
      601 gtcgagattg ccgtaatcga gaaaggtgct actgtcggtg cccacatcct ttcaggcgcc
      661 gtaatggatc cgcgtgcctt gcgagagctc atccctgact tcgtcgaaca aggcgcgcct
      721 ctcgagtcgc cggtgaacga agactttttc ctatacttga cagcgaagca cgccgttcgt
      781 tcgccgatca ctccaccacc attaaagaat catggctatt acatcgtttc gctaaaccgt
      841 cttacggcgt ggcttggtga gaagtgtgaa gaggtagggg taaacatatt tcccgagttt
      901 ccaggcgcgg aattgttatt caatgattgc gatcgggtcc ttggtgtaag gacaggtgat
      961 aaaggaatcg acaaggaagg gaagcagaag gcgaactatg aaccaggtgt ggacctccta
     1021 gccaaggtta ctgttttggg cgaagggccg cgcggttctc tgaccaaaaa actgatcgag
     1081 cgtctaggtc ttgatgaagg taaagagcca caggtataca gcatcggagt aaaggaactt
     1141 tgggagttgc cggacgaccg ttatccgact ggtcgcgtga ctcacacact tggttttcca
     1201 tccgactcac aaacgtacgg tggtggctgg atctacggaa tgcagaatcg cattctcaat
     1261 attggttacg tcaccggact cgattacaag gatccaatgc tcgatcccca cgcagagttt
     1321 cagcgctaca agacacatcc ttacatcgcg aaacttctgg atggcggcaa gatgatccgt
     1381 tatggcgcca agacgattgc ggcaggtggt tactttgcca tgccccgcac gtttgctgat
     1441 ggagttttga taacgggcga ttcggccggt cttttaaatt cgcaacgatt aaaaggaatt
     1501 cattcggcaa tcaagagcgg gatgcttgcc gcgcaatcga tatttcaagc tttattggcc
     1561 caggattatt cagcgtcgca actcaagagt tatgagcggg gcttgcagga tagctggatc
     1621 attcctgaat tgcgcaaagt caggaatttc cacgcgggct tcaaacgtgg acgttggctg
     1681 gggattgtta atgccgggct gcagttcttt actgggggac gagcgtgggg aatcctcgat
     1741 cgcgacaagg cacatgctgg tcaccttgag atgaaaaaac tctccgccta cggctaccgg
     1801 ggcgaccgtt tggagcagcg atacgatggg ctaaagttcg atggcaagct gacctttaat
     1861 aaagtgacag acgtttatca cgcagctgtt ggtcacgacg aggatcagcc tgctcactta
     1921 cacgtgttgg acacaaacat ctgctcgaca cgatgtgtcg aagagttcgg aaatccctgc
     1981 caacgattct gtcctgcggc cgtttatgaa atggttgatg aaggcgcgac aggccgaaaa
     2041 cgcttgcaga taaacttctc aaactgcgtc cactgcaaga cgtgcgacat catggatccc
     2101 taccagatta ttaactgggt gacgccggag ggtggcgggg gaccagacta taaaggaatg
     2161 tagccgatct tattcggcgg gcgactgcgc atgcccgtcg tgtttaatgg tgaagtgtac
     2221 tcgaccgcga atgacttctt ccaacgcgat tcgggtgggc ttgtgctttt gcggatcaat
     2281 ttcgattcga ggttgagctc ctcgttgcaa ctgtttactg cgttttgccg cgaggattat
     2341 tagacgatac ttcgaatcta cttcaggtgt ttcgcggccc tctatttcgg gtactactag
     2401 ctcggtcgat gtcggcatgc tactcccttt caaattactt tcgatcatct aatttaaact
     2461 gttcgattac ggcgttaatt aaggtctcct ggcggacgca gcgcgctcgt tcagcgtcga
     2521 tgatcgatgc aagttgagcc gcagcgcgat cgagccggtc gttcaggatg acgtagtcga
     2581 acgcggtata ttgactgagt tcctgagggg cattgcgcag ccgaacctca agttcttccg
     2641 gggaatcggt tccgcgggcg attaatcgat cccgcagcac ttcgtaagac ggcggcagta
     2701 taaaaacgct gatagagtcg accagcagtt tgcgaacact tgctgcgccc tgcacgtcga
     2761 cctcaaggat aatgtcgacg ccattggctg tctcatcgag cacttgtcgc tttgccgttc
     2821 cgtataagtt cccgtggaca ttggcccatt ccaaaaactc cccggcagca accatttctc
     2881 cgaaggtatt tctatttaca aagaaatact cgcggccttc aacttcacca ttacgaggcg
     2941 cgcgagtagt gtaggagaca gagtaactta gattcggcac tcgcacaacg tcaagcagcc
     3001 tccgaatgat gctccctttc ccgccaccag acggcgagct caccacgaag agagtcccac
     3061 ggggcgctcg gcccgagatt gttttttgcg aaactacctc agtcactcta ctcgacattc
     3121 tggacctgtt cgcgaagttt ctcgacctcg gccttgatcg ccaggccagc ttccttgatc
     3181 gtcaaatctg ttgattttga tagagttgtg ttcgcttcgc gattcagctc ctgcaataag
     3241 aaatcgagca ttttacccgt ttctccctgc gcttccagtg cttcctggaa ctgggaaagg
     3301 tgactacgca accggaccat ctcctcactt acatcactgc ggtcggccag gtatgcgact
     3361 tcctgagcca atcgcgccgg atcgatttcc actacctgtc cgcctcgatt caacaactcc
     3421 gcaattcttt tttggaggcg ctggcggtaa gcatccgcca gtcctgcggc cgcgctctca
     3481 attgtcggta caagcccttc gattcgtccg acacgctcac ggagttccag acgcaacgcc
     3541 tcgccttcct gttggcgcat cttctcgagc tcgtcaagcc cgaggtccaa agcttgttcg
     3601 agagctacac cgatgcgttc atctggaccg ctcttagctg gttggagcgc accgggaatt
     3661 cttgcgagga cgttgatatc gatgtcccca ccgataccaa agtcttgttg catttggcgc
     3721 agtgcggcaa cgtaacccgc tatcaggggt cggtttagtt cgtaggctgt ttgcgttgtg
     3781 cgctcgacgc tgaccgctat atctacccga ccacgtgaca gtcgagaact tatacgacgt
     3841 ttgatgactg gttcaaatga agaccattcg gttcccacgc gcaagtgaat atcgaggaaa
     3901 cggttgttaa cggtcttgag atctacggac acggagaaat cgtctgcaga tactgttccc
     3961 ttgccatagc cggtcattga tttcatagcg ttagattaaa gttgcgggtc gggttttact
     4021 ctcggacacc agccagcagt tcctcttcat ctgcaaattg aagttcgtac aacttgcgat
     4081 actgaccatt cttactcaga agttctgcat gcgtgcccat ttcaataatc ctgccagctt
     4141 tcatcactac aatcaggttt gcgcgtctca cggttgagag ccgatgggct attacgattg
     4201 tggtgcgatc tcgaaccaga ttgctaattg cccgttgaac taatctttcg gactcggcgt
     4261 ccagtgctga agtagcttca tccaaaatga ggaccggggc attaacaagg atcgcgcgag
     4321 cgattgcaag tcgctgacgc tgtcctccgg ataaaaaggt gcctcgctca ccgacaatgg
     4381 tgttgtagcc atctggcatc tctttgatga aatcgtgcgc gaacgcaatg cgcgccgctt
     4441 gttcaatttc cgcgtcacta gcttccggtt tcccgtacgc aatattgtga cggactgtat
     4501 cgttgaaaag aacagtctcc tgagtaacga gtgcaatttg agatctcaaa ctcgaaagcg
     4561 tgacatcacg caagtcggtt ccgtcccaga gaaccgagcc ggacgatgga tcgtgaaaac
     4621 gcggcaataa cttggtaaga gtcgatttgc cgccacctga ttcaccaacg agtgcgacca
     4681 ttgcgcccgc aggaatattc aggttgacac cgcgcagaac tgcgcgcgtc tcgttgtcgt
     4741 acccgaaaaa gacattgtct aaaactattt ttttctgaag cggcgccagt tctcgagcat
     4801 ccggcttttc cttaagcgcg gagtgttcgt tcatgacctc ccacacgtga tgcgccgcgg
     4861 cgaatgcttg ttccaggctg ttttgaagtc gcgaaagttt gcggaccggg tcgtagctgc
     4921 gaaacagaaa gaaaatgtac gtcaggaact gagcggtgtt cattcgacca gcgataattt
     4981 cgcgctgtcc gaagaacagc aggaaaacgg caa
//
LOCUS       DASSFP010000128         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_922071, whole genome shotgun sequence.
ACCESSION   DASSFP010000128 DASSFP010000000
VERSION     DASSFP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922071"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1148)
                     /locus_tag="VFH91_02735"
     CDS             complement(9..1148)
                     /locus_tag="VFH91_02735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6861926.1"
                     /translation="MLKRILLLSASAGAGHVRAAQALEKAFKQHFSERGEDSEIRHVD
                     TLDYTNKVFRHLYSKAYIDLVNKLPEVPGWIYDKLDKPWKNERRRLALDKLNTRPFVK
                     LLREYHPDMIVCTHFLPAEIVSWLKAKERLNSRQAIVVTDFDVHAMWLVHHYEHYFVA
                     VEESRVYLEALGIPANKITVTGIPIDPVFAQKKDQKQMRIKHGLDPDRTTILLSAGGF
                     GVGSVEALIDSLLPLSHPVQVVAICGRNEELKMRLQKSAKLRSNSNVLVKPVGYTIEM
                     DEFMSASDIVIGKPGGLTTSEALAKGLAFVIVNPIPGQEERNSDHLLEGAVGIRCNNL
                     PTLAYKLDRLLADPKRFAAMRKNARLMGRPNAARGIVEKLDQLGI"
     gene            complement(1160..1951)
                     /locus_tag="VFH91_02740"
     CDS             complement(1160..1951)
                     /locus_tag="VFH91_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6861927.1"
                     /translation="MELGLKDKVALVAASSRGLGRAVAEELAIEGASLILCARDAGQL
                     EQTAGSIRKSTGTRVLSVAADVSLPGDVSRVVDSGISEFGNIDILVTNAGGPPAGRFE
                     FLSHAEWESAVRLTLFSAVELIRGVLPSMKARKWGRILNITSIAVKQPVDNLMLSNSL
                     RSAVTGFARTLANEVAAEGITVNNILPGYTRTERVEELAQMMAEKEQISAGAFKSSWE
                     NQIPMKRLGEPREFAALAAFLVSERASYITGTSIQVDGGWTKSLF"
     gene            complement(1961..2335)
                     /locus_tag="VFH91_02745"
     CDS             complement(1961..2335)
                     /locus_tag="VFH91_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6861928.1"
                     /translation="MEQRTVTFYRWADMPKERVSDTLERRLITGDRIMLTHVYLKKGS
                     VVPHHNHENEQVTYILEGGLRFWIGEDESEVIDVMKGDVLHIPSFVHHKALALEDTLD
                     VDIFSPPRQDWLDKTDDYLRQK"
     gene            complement(2602..3051)
                     /locus_tag="VFH91_02750"
     CDS             complement(2602..3051)
                     /locus_tag="VFH91_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6861929.1"
                     /translation="MDKPKFVYVSYIESTPEKVWNALSDPEMTRRYWFNHENVSDWSV
                     GSKWQHVLKGENDMVHVVGKVLESDPPRKLVVSWGRPVDEANEAKHSRVTYLLEALEK
                     SVRLTVTHEEFGDDQKMLESVSNGWPAVISGLKTLLETGRALPAKMN"
     gene            complement(3053..3373)
                     /locus_tag="VFH91_02755"
     CDS             complement(3053..3373)
                     /locus_tag="VFH91_02755"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6861930.1"
                     /translation="MVTDIDRVFKALADPSRRRLLDLLHAENGQNLGELCAHLDMTRQ
                     AVTKHLVLLEDANLVVTVWRGREKLHYLNPMPINEIFDRWVGKYERRQVRALADLKKA
                     LEEN"
     gene            complement(3779..4405)
                     /locus_tag="VFH91_02760"
     CDS             complement(3779..4405)
                     /locus_tag="VFH91_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6861931.1"
                     /translation="MTNTEVFPVWPTNVAAKSTEKPAPLSDHALAIAAGQGDMAAFEQ
                     LYERHNRRVYSLCLRMTQNAAEAEDLAQEAFIQLFRKIGSFRGESAFTTWLHRLTVNQ
                     CLMHFRKKSVKLERTTEEGDTPVQVVRGTENPNAMPVLDRIALDNALKQLPAGYRTVF
                     VLHDVEGHEHEEIAKMLHVAVGTSKSQLHKARMKLRKILKSQRAVRRN"
     gene            complement(4601..>5617)
                     /locus_tag="VFH91_02765"
     CDS             complement(4601..>5617)
                     /locus_tag="VFH91_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6861932.1"
                     /translation="PDVKSSEMTLRSRLNAVLKELIMITGKYRSLAAASWVAVVLCMG
                     SLAFGQVTSPQTTAPQAASTTARSIPSGAKMKFKGVVITRDADTFTIRDRTRTDYQVL
                     LTDNTSIKTYGGFFKRGKDYAVTDILRGLIVEVEGRGDAQGQLVADKIRFNESDMRAA
                     ITTDTRVNPVEANQQKIAGQIDELYAVAAEARSEAANANERISSLDDYDMQESVAVTF
                     RTNSAVLSPEAKQTLDGLAGRITGQRAFMIEVAGYTDSTGGDAKNFRLSRDRADAVVQ
                     YLAVNHKIPLRRFVTPMGYGKTESVADNTTASGRAQNRRVEVRMLMNRGMSQRPVTTS
                     SAKP"
BASE COUNT         1318 a         1513 c         1312 g         1474 t
ORIGIN      
        1 gcaaagcact aaataccaag ctggtccagc ttttcaacta ttcctcgcgc ggcattgggc
       61 cggcccatca accgcgcatt tttccgcata gcagcaaatc gctttggatc ggcaagcaat
      121 ctatctaact tataagcaag cgttggcaaa ttattacagc ggatgccgac agcgccctca
      181 agtaagtggt cggagttgcg ttcttcctga ccaggtattg gattcacaat cacgaacgca
      241 agtccctttg ccagtgcttc cgaagtggtg agaccgcccg gcttcccgat aacaatgtca
      301 gaagcagaca tgaattcgtc catctcaatt gtgtacccaa caggcttcac cagtacgttg
      361 gaattgcttc ttaacttcgc tgacttttgt agccgcatct taagttcttc gtttcgaccg
      421 catatcgcca ctacttgtac gggatgactt agtggtaaca acgagtcaat taatgcctcg
      481 actgacccga ccccgaatcc acctgccgac aagagaatcg tcgttctatc cgggtccaaa
      541 ccgtgcttaa tgcgcatttg cttctggtcc tttttttgcg caaacaccgg atcgatggga
      601 atgccagtga ctgtgatttt gttagccggg atccccaacg cctccagata cacgcgtgat
      661 tcttctaccg ccacgaagta gtgttcatag tgatggacca gccacattgc gtgaacatca
      721 aagtccgtga ccacgattgc ctgacgacta ttaagtcgct cttttgcttt taaccaggag
      781 acaatttccg ccggcaaaaa gtgcgtacag acaatcatgt ctggatgata ttcgcgcagt
      841 agctttacaa aaggacgcgt atttaacttg tcaagcgcaa gtcttctccg ctcgttcttc
      901 cagggcttgt cgagtttgtc gtaaatccat cccggcactt cgggaagctt gttcaccaga
      961 tcgatgtagg cctttgaata gagatgccgg aagaccttgt tcgtgtaatc aagagtgtcg
     1021 acatgacgga tttcgctatc ttcacctctt tcggagaagt gttgcttaaa agctttctcg
     1081 agtgcctgtg cggctctgac atggcccgct cccgcggacg cggaaagcaa taggattctt
     1141 ttcagcatat tctaagtggc taaaagagag actttgtcca gccgccatcg acctggatcg
     1201 aggtgccggt gatgtagcta gcacgttcag aaacaagaaa ggccgccaaa gcagcgaact
     1261 cgcgcggctc gccgagcctc ttcatcggaa tctggttttc ccaactcgac ttgaaagcgc
     1321 cggcgctaat ctgttctttt tcagccatca tttgtgccag ctcctcaact cgctccgttc
     1381 ttgtatagcc aggtaagatg ttgttgaccg tgattccttc agccgcaact tcattagcta
     1441 gtgttcgtgc gaatcctgtt acggctgaac gcaagctatt cgagagcatc agattatcta
     1501 cgggctgttt tacggctatc gacgtgatat tgagaatgcg tccccacttc ctcgctttca
     1561 ttgacggtag cacaccacga atgagttcaa ctgcgctaaa aagagtcaaa cgaacagcag
     1621 actcccattc cgcatgactc aaaaactcaa atcgtccggc aggtgggcca ccagcattcg
     1681 ttaccagaat atcaatgttt ccaaactcac taattccaga atccacaaca cgacttacat
     1741 cgcccggtaa tgaaacatcc gccgcgactg aaagaactct tgttcccgtc gacttcctaa
     1801 ttgagcccgc cgtttgttcg agttgcccgg catctcgggc gcaaagaatt agtgaagcgc
     1861 cttcaatcgc aagttcctcg gccactgcac gaccaagccc gcgactggac gccgcaacca
     1921 gggcaacttt gtctttgagt ccgagttcca tcttccgcag ctatttctga cgtaaataat
     1981 cgtcggtctt gtcgagccag tcctgtcttg gcgggctgaa tatgtccaca tccagggtat
     2041 cttcgagcgc gagtgctttg tgatgtacaa aagacggtat gtgaagcaca tcgcccttca
     2101 taacatctat tacttcggat tcgtcttcgc ctatccagaa tcgaagtcca ccttcaagaa
     2161 tgtaggtgac ctgttcattc tcgtgattgt gatgcggaac cactgagccc tttttcaaat
     2221 acacgtgtgt aagcatgatg cgatcgcccg taataagcct tcgctccaat gtgtcggaaa
     2281 ctcgctcctt tggcatgtct gcccaccgat agaacgtaac agttcgttgc tccatttact
     2341 tgctcctgag atttttggat gcttttgacg ccgtcgattg attagcggtt cgacgcggag
     2401 aggtaagtta acaagacacc gtgaagatta ccagaacttt ggagagcgtg ggctttgcgc
     2461 cgctttgtta tatcgatccc accggtaatg gaaagggccc gacgcgtgtc cgctttcggt
     2521 ctccgcgccg ggccaatttg taacactacc tgctattcgg taaggagctt aaaaagcgcc
     2581 gccgggccgg cgcactccaa attaattcat ctttgcgggt agggctctgc cagtctcaag
     2641 cagcgtcttc agaccggaga tcaccgcagg ccatccattc gaaacgcttt ccagcatctt
     2701 ctgatcatca ccaaactctt catgagtgac tgttagcctc actgatttct cgagggcttc
     2761 aagtagatag gttactcgcg aatgtttggc ctcgttggct tcatcaactg gacgtcccca
     2821 gctaacgact aatttccgag gtggatcaga ttccagcacc ttaccgacaa catggaccat
     2881 atcgttctcg ccttttaaga cgtgttgcca ctttgatcct accgaccaat cagagacgtt
     2941 ttcgtggttg aaccagtagc gtctggtcat ctctggatcg cttaacgcat tccacacttt
     3001 ttcgggagtc gattcgatat agctaacata aacgaatttt ggcttgtcca tctcaattct
     3061 cctcaagagc tttcttcaga tcagcaagcg ctcggacctg ccggcgctca tacttgccta
     3121 cccaacgatc aaagatttcg ttaattggca ttgggttcag atagtggagc ttttcgcgtc
     3181 cacgccatac agtcacaact agattcgcat cctcaagcag taccaagtgt ttggttacgg
     3241 cctgtcgtgt catgtcgaga tgcgcacaga gctctcccag gttttgaccg ttctctgcgt
     3301 gcagaagatc gagcaaacga cggcgacttg gatcagcaag cgccttgaag actctgtcga
     3361 tatcggtgac cattcctcat tatgcaacca aatggttgca taatgcaact gggactctct
     3421 gaatctacac accgtcagtt tcttattcgt gttatttcgt gtgatttggt agatcgcctg
     3481 atatggtccg agaaaagcga tccacgcgca tcacacgaaa ctacacgaac aagaaactga
     3541 gagtgcttcc cggacaccac cgactttcag tcgcgtggta cttcaacttt tgcttagctc
     3601 gctcacgaca gacttctctt ccacaggatt ccatcaaacc gcagctccct ttaggtcgaa
     3661 attgaactac cacctgactg aaaagcgatg gtgtgatgta tttgtccgta cccgtgcacc
     3721 caaaaaaaca aaaggcctac ctagttaggc aggccacagt attaagttca atgctaaatt
     3781 aattacgtcg cacggcacgc tgcgacttca gaatcttgcg caacttcatc cgagctttat
     3841 gtagttgcga tttcgaagtt ccaacggcca cgtggagcat ctttgctatc tcctcatgct
     3901 cgtgaccttc gacgtcgtgc agcacgaata cagtgcgata acctgccggc aattgcttaa
     3961 gtgcattgtc cagggctatt cgatccagca ccggcatcgc attcggattc tctgtcccgc
     4021 gaacaacttg aaccggggta tctccctctt cagtcgttcg ttcaagcttg acgctcttct
     4081 tgcggaagtg cattaaacac tggttcacag ttaacctgtg tagccaggtt gtgaacgccg
     4141 actcccctct gaaacttcca atcttacgaa agagctggat gaaagcctcc tgagcaagat
     4201 cctccgcctc agctgcgttc tgagtcatgc gaagacatag tgaatacaca cggcgattat
     4261 gtcgctcata cagctgctcg aaggctgcca tgtcaccctg tccggccgcg atcgctaaag
     4321 catgatcgga aagcggtgcc ggtttttcag tcgacttcgc cgcaacattg gtcggccata
     4381 ctgggaagac ttcggtattt gtcatggcct ctcttagtgc aagccgaggg ccacgccaaa
     4441 agacctgaaa actaccaatt tttggggtga tcaacagaat ttatccacga aagagggtgc
     4501 aaggagacga aagcgtgtag cgttttattc cgaacgacat ttgacgcctg ttgtcgtact
     4561 tttccaatga aaacgacaac aggcgtcagt cggctttgga ctatggtttt gctgaggatg
     4621 ttgttaccgg acgttgactc attccgcggt tcatcagcat gcgaacttca acgcgccggt
     4681 tttgcgcacg acccgaagcg gtcgtattgt ccgccacaga ttcagtcttt ccgtaaccca
     4741 ttggcgtcac gaatctgcga agcggaatct tgtgattgac tgccaggtac tgaactaccg
     4801 catctgcacg gtcacggctc aacctgaagt ttttcgcatc acctccggtg gagtctgtgt
     4861 atccagccac ttcaatcatg aatgcccgct ggccggtaat cctgccggcg agtccatcga
     4921 gcgtttgttt ggcttctgga gacagcaccg cgctatttgt cctgaatgtg acagccacgc
     4981 tttcctgcat atcgtagtca tctaatgacg atatgcgctc gttagcattc gcggcttcac
     5041 tgcgagcctc ggctgccact gcataaagtt cgtcaatttg cccagcaatt ttctgctgat
     5101 tggcttcaac tggattaacg cgcgtgtccg tcgtaatcgc agcgcgcatg tccgactcat
     5161 taaatcggat cttgtctgcg actaactgtc cttgcgcatc accgcgtcct tcaacttcca
     5221 cgatcaaacc acgaaggata tcggtgactg cgtaatcctt tccacgttta aagaagccac
     5281 cataggtttt gatgctggtg ttatcagtga gtagaacctg atagtcagtt cgtgttcgat
     5341 ctctgatagt gaacgtgtct gcgtcacggg ttatgacgac acctttgaat ttcatctttg
     5401 cgccgcttgg aatcgaacgg gctgtcgtac tggccgcctg tggagcagtt gtttggggcg
     5461 aagtgacttg tccaaaagcg agggaaccca tgcataggac tacagcgacc caagacgctg
     5521 cagccagaga acgatatttg ccggtgatca tgattagctc cttcaaaaca gcattcaggc
     5581 gacttcgaag agtcatctct gaagatttaa cgtcggg
//
LOCUS       DASSFP010000129         5953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_770026, whole genome shotgun sequence.
ACCESSION   DASSFP010000129 DASSFP010000000
VERSION     DASSFP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770026"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFH91_02770"
     CDS             <1..446
                     /locus_tag="VFH91_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET6861933.1"
                     /translation="AICFGMQILNVARGGTLIQDIGSQVPEGIKHEQGPPRDRWSHSV
                     RVMEGSKLARMTDNEKLLVNSHHHQAVERVGENLVATAWSNDGLIEALEDPRSERFVV
                     AVQWHPELGWEKDKVSQRLFGSLISAAGQNVNVKGQVSDSISITV"
     gene            462..1244
                     /locus_tag="VFH91_02775"
     CDS             462..1244
                     /locus_tag="VFH91_02775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET6861934.1"
                     /translation="MKPENKHGPTGLSNRLAIVTGGSRGIGRAVVELLAGAGADVVLN
                     FVNDDQAANAAVSTAQSNGVKASAVKADISILSEAEKLVEETLKQFDRIDFLICNAGI
                     WKGANVEVMSEALWDETLDINLKGTWAMCRAVVPVMKRQGFGRIVIVSSTAGQRGEAN
                     YSNYAASKGGQISFAKSLASELGPFGINVNCVAPGWVNTDMSAGALSDPRELNSILKL
                     IPVGRVAEAEDIAGPIVFLCSDWAKHISGEVLNVNGGSVLCG"
     gene            1293..2480
                     /locus_tag="VFH91_02780"
     CDS             1293..2480
                     /locus_tag="VFH91_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET6861935.1"
                     /translation="MTSSEKQHVELGLETLLEARGDLLGDLRVGLICNQASVNHHFRH
                     AADLFHEHQQINLRVLFGPQHGIRGDVQDNMIETGHAIDRATGLPVYSLYSETREPTE
                     EMLKDVDVLVFDMQDVGCRIYTFVYTLANCMKSAVRFGKKVIVCDRPNPINGVGVSGN
                     VLEPEFASFVGQYPLPTRHGMTVGELALMFNQHFNIGCDLEVIPATGWRRELWFDDTD
                     APWVLPSPNMPTVESATVFPGTVHFEGTQMSEGRGTTRPFELIGAPYVEPEAYADELN
                     ALNFEGVYFRSCVFQPTFQKHAKVSCGGVQIHVTDRNLFEPVSVGIGMVKTAYDMYPD
                     QFLWKEPPYEYVFDRNPFDVICGTDATRKAIESGISLTETIDNWQKPLNDFKVIREGF
                     LLY"
     gene            2641..3075
                     /locus_tag="VFH91_02785"
     CDS             2641..3075
                     /locus_tag="VFH91_02785"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HET6861936.1"
                     /translation="MVKAEKIELERHEPTPGSAWLIGAVAWFVPGVGHLLLKKWWRAL
                     LMGGAVWLCFFLGLIMGGHMFDLSTGQGSSTILQVPPMIANLGSGILYVVSWLMGVGF
                     ADNPESARLATYEYGNTFLLIAGLLNYLTMLDAFDVAAGRKP"
     gene            3072..3239
                     /locus_tag="VFH91_02790"
     CDS             3072..3239
                     /locus_tag="VFH91_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861937.1"
                     /translation="MRHFLVMTLFAALASVVFGSVAKETPNERVIYGVKVFAEFMVIG
                     LLLSWILYFIP"
     gene            3245..3988
                     /gene="recO"
                     /locus_tag="VFH91_02795"
     CDS             3245..3988
                     /gene="recO"
                     /locus_tag="VFH91_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET6861938.1"
                     /translation="MALLDSEALILRSYNLAEADKIIVSLSRSAGLLRGVAKGSRKLK
                     NRFGASLEPFTLVNLTWYEKENQELVSIRQTEILRSYFKLSCDANVVNSLAYMGDLLI
                     EFSPPRQANDTLFRMVAACLEAIAGSPADLESIVRYFEVWLLKIEGFLPDLKRCPDCL
                     RVFGVEAVYMGNDLALRCRDCSQGRGGTISKSLHAHLRNTESLAPARFAETARDVSGI
                     TKRELGELTHQLIGRVLERLPRVRPTFVQ"
     gene            4016..>5953
                     /locus_tag="VFH91_02800"
     CDS             4016..>5953
                     /locus_tag="VFH91_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET6861939.1"
                     /translation="MNFRRTLIPRYVIGAVLPYVFLSLLLLTSVLFAQQASRLAEAII
                     YTDLPFSLLAGIGATLLPGVLTFTIPLATLAGIAIGFSRMGTDSEIVAMRAAGIGSWA
                     LIWPALLVGLFLSGASVYIQLFEAPRAARELEKVALEGALAKLDSPVEPRTFTSLPKN
                     VIYVRDGDKARGSWGRVFISTQQDDGSTRLFTARSGRIDSSGDQSELVLGDVLATKFP
                     GDESKDRKSYVVERFDQLRLAINTGRADIVQRLNQRDSNADSLDWSELRERSASGTRA
                     QRREARRILNRRAALGTAPLVFAFLAVCLGLRIRRGGRSTGILISLVVAIVYYLLSLL
                     GESLSRAGSETPQMGAWFPTAFLILMSLFFLWVKRIPFLSSISLKGRAKDQSSTERQT
                     VRTHGTTSWRLPNLMDASLFRSLTVTFCLGFLALVSIFNIFTLFELWRFIAVRHSSAA
                     LVARYLLFLLPLVSVELFPATMLIAVLVTYALLAKRHEAIAWWASGQSVYRLMFPGLF
                     FAVGIALCSWLIQEHLMPNANLKQDALRARIKGDDPRVITGTGRQWLASIDTRRLYSY
                     EFDEKGNLREPTIYELDAEAVHLSKVVSADVGTWVDPSHLKLLNSETLSLKGMEVEKT
                     IEPETTLDSVESPQIFKPSIDK"
BASE COUNT         1391 a         1339 c         1608 g         1615 t
ORIGIN      
        1 tggcgatctg ttttggaatg cagatcttaa atgtcgcacg aggcggcacg ctcattcaag
       61 atattggctc acaagtccca gaaggcatca agcatgagca gggcccgccg cgcgatcgtt
      121 ggtcacatag tgtgcgggta atggaaggaa gcaagctcgc tcgcatgact gacaatgaaa
      181 agcttttggt aaatagtcat catcaccagg ccgttgagag agtaggagag aatctggttg
      241 caactgcctg gtccaatgat ggtttgatcg aagctttgga ggacccgcgc tcggagcgtt
      301 ttgtggtcgc agttcaatgg catccggagt taggttggga aaaggataaa gtctctcaac
      361 ggctgtttgg ctcactcatc tcggctgctg gtcaaaatgt taatgtgaaa ggtcaagtat
      421 ctgactctat ttcaatcaca gtctagtact taattaggta aatgaaacct gagaacaaac
      481 atggcccaac gggcttgagt aatcggttag caattgtaac cggaggttca agaggaattg
      541 gtcgagcagt ggttgaacta ttggccggtg ctggcgctga tgtcgtcctc aactttgtaa
      601 atgacgatca ggcggctaat gcagcagttt caaccgcgca gagcaatggc gtaaaggcca
      661 gcgctgtaaa ggctgatatt tcaattttaa gtgaggcgga aaagctagtt gaagaaactc
      721 ttaagcagtt cgaccgtatc gactttctca tctgcaacgc cggaatttgg aagggagcca
      781 acgttgaggt tatgtccgag gctctatggg acgaaacgtt agacattaat ttgaagggca
      841 cgtgggccat gtgccgcgcc gtagttccag tgatgaaacg gcaaggcttt ggcagaatcg
      901 ttattgtcag ttcaaccgcc gggcagagag gagaagcgaa ttattccaat tacgcagcct
      961 ccaaaggtgg acagatctcg tttgcgaagt ctctcgcctc tgaacttggg ccttttggta
     1021 tcaacgtgaa ctgtgtggct cctggctggg tgaacacaga tatgtcggcg ggcgctctct
     1081 ccgacccacg ggaactgaat tcaattctga aattgatacc tgtcggacgc gtggccgaag
     1141 ctgaagacat cgcgggaccg atagtttttc tttgctcaga ttgggcgaaa cacatttcag
     1201 gagaggtact gaatgtcaat ggaggttcag tgctctgcgg atagaaccga aagtaaagta
     1261 tgactttaac ttttgaaaac actaatgcgc gtatgactag tagcgagaaa caacacgtag
     1321 aactcggact tgagacatta cttgaggcga ggggtgattt gctcggcgat ttgcgagtcg
     1381 gtcttatttg caaccaagca tccgtaaatc accacttccg ccatgcggcc gatctcttcc
     1441 acgagcatca acaaatcaat cttcgcgtct tgtttggtcc acagcacgga attcgtggtg
     1501 atgttcagga caatatgatc gaaaccggac acgcgataga ccgtgcgacc ggattacctg
     1561 tctattcact ttacagtgag actcgggagc ctacggaaga gatgctcaag gatgtagatg
     1621 tgctggtttt tgacatgcag gatgtcggat gtcgcatcta tacctttgtc tatacgttgg
     1681 cgaattgtat gaagtcggct gttcggttcg gaaagaaagt aattgtatgt gatcgaccca
     1741 acccgattaa cggtgtaggc gtctcaggta atgtgttgga gcccgaattt gcttcgttcg
     1801 taggtcaata tccattgcct actcgacatg ggatgactgt tggtgaactg gccttaatgt
     1861 tcaaccagca ctttaacatc ggctgcgacc ttgaggtgat tccggcgact ggctggagac
     1921 gcgaactatg gtttgacgat acagatgcgc cctgggtttt gccatctcct aatatgccta
     1981 ccgttgaatc tgcgacagtt ttcccgggaa cagttcactt cgagggcact cagatgtctg
     2041 aagggcgtgg aaccacccgc ccgtttgaac ttattggcgc gccttacgta gaacctgagg
     2101 catacgcgga cgagttaaat gcgttgaatt ttgaaggtgt ttactttaga agttgcgtct
     2161 ttcagcctac attccaaaag catgcaaagg ttagttgtgg aggagttcag atacacgtta
     2221 ctgatcgaaa tcttttcgag ccagtaagtg tgggaatagg gatggtaaag accgcatatg
     2281 atatgtaccc ggatcaattc ctttggaaag aacctccgta tgagtatgtg ttcgacagga
     2341 atccgtttga tgttatctgc ggaacggacg caacgcgcaa agcaattgaa agcgggatct
     2401 cgctgacgga aacaattgat aactggcaaa agcctctcaa tgatttcaaa gtgattcgtg
     2461 aagggttctt gttgtattaa ttatttcttt gggttaagtc tggcgagatc ttaaggtgga
     2521 ttattaaacg tcgtgttgcc gacggttcag atctttcttt aggcacgcaa tggccgcata
     2581 gggttgcgag taagtttcgg ttcaggtaca actttaacga acaagcagtt ggaggcagag
     2641 ttggtaaaag ctgaaaagat cgaattggag cgacacgagc ctactccagg ttcggcgtgg
     2701 cttatcgggg cggtggcctg gtttgtcccg ggcgttggcc atttgttgtt gaagaaatgg
     2761 tggcgagctc ttttgatggg aggagcggtc tggctttgtt tcttccttgg gctgatcatg
     2821 ggaggtcaca tgttcgacct ttcgacgggt cagggatctt cgaccattct tcaagttccg
     2881 ccgatgattg caaacctggg atccggaatc ctttatgtag tgtcatggtt gatgggtgtg
     2941 ggctttgcag ataatcccga aagtgctcgt ctcgctactt atgaatatgg gaatacattt
     3001 ttattgattg ctggtttact taattatttg actatgcttg atgcatttga tgtcgccgct
     3061 gggaggaagc cttgagacac tttcttgtta tgacgctctt cgcggccctg gcctcggtag
     3121 tctttggttc ggtagcaaaa gagacgccaa atgaacgcgt gatctacggc gtaaaagtct
     3181 ttgcagaatt catggtcata ggtctgctct tgagttggat tctttatttc attccttaac
     3241 tccaatggct cttctcgata gcgaagctct gatcctgcgt tcctacaatc tagccgaagc
     3301 agataagatc atagtttcgt tgagtcgatc tgctggtctc ctcagaggcg tggcaaaggg
     3361 ttcccgcaag cttaagaatc gttttggggc ctcgcttgaa ccattcacac tcgtaaacct
     3421 gacgtggtac gagaaggaaa atcaagaatt agtttccatt cgacaaacag agatcttaag
     3481 gtcctacttt aagctttcgt gtgatgcaaa cgtggtgaac agcctcgcat acatgggcga
     3541 ccttttgatc gaattctcac ctccccggca agctaatgac acactgtttc gaatggtcgc
     3601 ggcttgtctt gaagccattg ctggatctcc ggcagacttg gagtcaatcg ttcgctattt
     3661 cgaggtgtgg ttactaaaga ttgagggatt tcttcctgac ttgaaacgat gtccagactg
     3721 cctcagggta tttggagttg aagccgttta catgggaaat gatctggcgt tgcgttgccg
     3781 cgactgtagt cagggtcgcg gaggaacaat ctcgaagagt ttgcatgccc atctccggaa
     3841 caccgagagc ttggctccag cgcgatttgc tgaaaccgct cgagatgttt ccggaattac
     3901 aaagagggaa ctcggcgagt tgactcacca gcttattggc agagtgctcg agcgactgcc
     3961 gcgagtcagg cctacatttg ttcaataact actcgaacta cacttgagga ttaacttgaa
     4021 cttccgtcgg accctaattc cacgctacgt aatcggcgcg gtgcttccat atgtctttct
     4081 ctcattgctt ctgctgacgt cagttctttt cgcgcagcag gcaagccgcc tggctgaggc
     4141 gattatctat accgatctgc cgttctcatt gctggctgga attggcgcaa cgctgctacc
     4201 tggcgtactg acttttacga ttcctttagc gactttggcc gggatcgcga tcggcttttc
     4261 gcgcatgggc actgacagcg agattgtggc gatgcgagca gcgggtattg gttcctgggc
     4321 gttgatatgg ccggcgttac ttgttggcct gttcctctct ggtgctagtg tttacatcca
     4381 attgttcgag gcgccacgcg cagctcggga gcttgaaaag gtagcgctcg aaggcgcgtt
     4441 ggcgaaatta gattcgccgg tcgagcctcg cactttcacc agtttgccga aaaacgtgat
     4501 ctacgtacgt gatggcgata aagccagggg aagctgggga agagttttta tctctactca
     4561 gcaggacgat ggttcgactc ggctctttac tgcccgttcc ggacggattg attcgtcggg
     4621 tgaccaatcc gagttggttc tcggcgatgt ccttgccaca aagtttccgg gcgatgagag
     4681 taaggaccgc aaatcttatg ttgtcgaacg attcgatcag ttaagactcg ccattaacac
     4741 tggtcgcgca gatatcgttc agcgtttgaa tcagcgggac agtaatgccg attcgcttga
     4801 ctggagtgag ttgcgcgagc gaagcgcttc cggaaccaga gcacaacgac gcgaagcgcg
     4861 gcggattcta aacagacggg cagctcttgg aactgcgccc ctggtctttg cttttctcgc
     4921 tgtctgctta ggtttgagaa ttcggcgcgg cgggcgcagt accggcattc tcatatcgct
     4981 ggtcgtcgcc attgtctact atctgctatc tctgctcggt gagtctcttt cacgtgcagg
     5041 atcggaaact ccacaaatgg gagcctggtt tccgactgcg tttttgatcc taatgagctt
     5101 gttctttctg tgggtcaagc gcattccttt cttgtcgtcg atatctctga agggaagagc
     5161 gaaggatcaa agctccacgg aacggcaaac ggtcaggacc catggcacga caagttggcg
     5221 cttgccaaat ctcatggatg cttcactgtt ccgatcgtta accgtaactt tctgtcttgg
     5281 gtttttagca cttgtatcca ttttcaacat cttcaccctt ttcgaactat ggcgattcat
     5341 agcagtcagg cacagcagcg cggccctcgt cgcacgatat ctcttatttc tgctaccgtt
     5401 agtttcagtg gaattattcc cggccacaat gttgattgca gtgcttgtca cctacgcttt
     5461 gttggcgaaa cgacatgagg ccattgcctg gtgggcatct ggtcaaagtg tctaccgcct
     5521 gatgtttccg ggtcttttct tcgccgttgg catcgcatta tgttcatggc taattcaaga
     5581 acacctcatg cctaacgcaa atcttaagca ggatgccttg cgcgcgcgta tcaagggtga
     5641 tgaccctcgt gtcatcacgg gcacgggtag acagtggttg gcatcgatcg acacccgccg
     5701 tctgtactct tatgaatttg atgagaaagg gaatttgcga gagccgacaa tttatgagtt
     5761 ggacgcagaa gcggttcacc ttagtaaagt cgtaagcgca gatgttggga cctgggttga
     5821 tccatcacat ttaaagttac ttaacagcga aaccttatct ttgaaaggta tggaagtaga
     5881 gaagacaatt gagccggaaa cgacccttga ttcagtcgag tcaccgcaga tctttaaacc
     5941 tagtattgat aag
//
LOCUS       DASSFP010000130         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1666477, whole genome shotgun sequence.
ACCESSION   DASSFP010000130 DASSFP010000000
VERSION     DASSFP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666477"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFH91_02805"
     CDS             <1..452
                     /locus_tag="VFH91_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6861940.1"
                     /translation="VSWNLKALDQRSAVRVRTGSESEMSEMVLIIEDDPDIAEIVRYT
                     LEKAHFLTRVAYSGEEGLQASLDKLNPPSVILLDLLLPGMTGHELCRRLRRETLTHNT
                     PILVLTAKAMEADINFALAVGATEVMIKPFSIDELVTRVRSLAQRGH"
     gene            678..>3350
                     /locus_tag="VFH91_02810"
     CDS             678..>3350
                     /locus_tag="VFH91_02810"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HET6861941.1"
                     /translation="MSKKLSKLFAFHLSLAILLSSLPGLSTALQLEAAPRRTATSPKT
                     HPLTPLVASVDLSTYVRVGRFDLPEPTRTVPPPNSLLAQEASALTYNWDTDTLFVVGD
                     GGTSVVQVTKTGQLIDSMTLAPGPSPQGTDFFDTEGVSYVGGGKFVLMEERYRQANLF
                     TYVAGGTLHKTDVQSVKLGTTIDNIGLEGISYDPLTSGYICVKEKDPESIFQTGIDFV
                     AGTATNGSPSATSSTDLFNPALANLADFSDVFALSNLPSLIGQPDYSHLLVLSQESGQ
                     IVNIDRTGNISSSLTIVSDPGNPLSVADQTDEGLTMDRDGNLYIVNENGGGDSAHPQL
                     WVYAPSNAPNQPPTAVTLNNAVTSIPENSSTAAPVKLADIIVTDDGLGPNHLSVTGVD
                     ASFFQIIGTALYLKAGTALSSTIKPSYSVTVNVDDPTVGSTPDASANFTLTVTAATGG
                     TASLVISEVAPWSSGNSPISLRVDWFEVTNTGNATANIAGWKMDDDSHSFNTAVALNG
                     ITNIAPGESVIFLETTDLAGKSAAFKTLWFGANPPANLQIGSYSGSGVGLSTSGDQVN
                     LFDSGGIQQAGVIFGASPSGPFPTFDNAAELNNATISLLSAAGINGAFVAPNDSTETG
                     SPGTIGAPSAPVVGITATDPSATETGNDSATFRITRNGSNVGPLTVSYTIATGAGQAT
                     SADYSPALTGVATIASGQSFVDIIITPVTDNLVEGSETVTLTLGDSGSYDVGANKTAT
                     VTITDNPFLGVAAGDANASSAVLWTRINRAQSATVTAQVSTDAAFSAASLTFSGTTDP
                     TKDNTLKIVATGLASGTRYYYRFIIDGTGETSRTGTFKTAPLANTAVPLHFAFSGDND
                     GLMRPYALANVIPSQHLDFYHNLGDVIYETASNLT"
BASE COUNT          811 a          913 c          842 g          784 t
ORIGIN      
        1 ttgtcagttg gaatttgaag gcattggatc aacgctcggc agtgagagtc cggacaggaa
       61 gtgaatctga gatgagtgag atggtattaa tcatcgaaga cgatcctgac atcgcagaga
      121 tagttcgtta cactctcgaa aaggcccatt tcctaactcg tgttgcttat agtggcgaag
      181 agggacttca agcttcgctc gacaagctca acccgccatc ggtgattctt cttgatctgc
      241 tgctacccgg catgaccggg cacgaactat gcaggaggct gcgtcgcgaa accttaactc
      301 ataacacacc gattcttgtt ctcactgcaa aagccatgga ggctgatatc aattttgctt
      361 tagctgtggg agccactgaa gtaatgatca agccattttc gattgatgag ttggttacac
      421 gtgtgcgctc tctcgcacag cgtggtcatt gatttccaac cgggaatatc actactcagt
      481 tcgtttgcga tagacataga aggtttgcct tccaattaat cccgaaacct cacgtcgcat
      541 ttgttcataa ggtttctaag aaaacttctc aaagaattaa catcgcccgc ttacactgcc
      601 ccgcttatca gcagtcattc cgttggaatt agggccttaa ttcttgggca cacccctatc
      661 aaatcaagga gatcaccgtg agcaaaaagc tctctaaact gtttgcattc catctaagtc
      721 tcgccatatt actgagttcg ctgcctggcc tttcgactgc gctccagctc gaggctgcac
      781 cgcgcagaac agctaccagt ccgaaaacgc atcccttaac accactcgtt gcgtcagttg
      841 atctgtcgac gtatgtcagg gtcggacgct ttgatctgcc cgagccaaca cggaccgttc
      901 cacctcccaa tagtctattg gcccaggaag cctcagcttt gacttacaac tgggataccg
      961 atacgttgtt tgttgtcggt gatggtggca cttcagtggt ccaggtcacg aagaccgggc
     1021 agttgatcga ctcgatgacg ttagcaccgg gccccagtcc ccagggtacg gacttcttcg
     1081 acacagaagg ggtttcttat gtcggtggcg gcaagttcgt tttgatggaa gagagatatc
     1141 gacaggccaa tttattcacg tatgtggcag gcggcactct tcacaagacc gacgtccaat
     1201 ccgttaagct cggcacgacc atcgacaaca tcggccttga aggcatctcg tacgatccgc
     1261 tgacgagcgg atacatttgc gttaaagaga aggatccgga atccatcttc caaacgggta
     1321 ttgatttcgt agcaggaacg gcaaccaacg gctctccctc agcaacgagc tcgaccgatc
     1381 tattcaatcc ggctttggcc aaccttgcgg acttttctga tgtcttcgcg ctttcgaatc
     1441 tcccgtcact gatcggccag cctgattaca gccacctgtt ggtgttgagc caggaatcag
     1501 gccagatcgt caacatcgat cgaactggaa acatctcgag ttcgttgacg attgtctccg
     1561 atccaggcaa tccattgtcg gttgcggacc agactgatga aggtctcacg atggatcgcg
     1621 atggcaacct ttacattgtg aatgagaacg gcggcggtga tagcgctcat ccccagctct
     1681 gggtatacgc accatccaac gctccaaacc aaccacctac agcagtgacg ttgaataatg
     1741 cagtcacttc gattccagag aactcgagca ctgcagcacc agttaaattg gcagacatca
     1801 tcgtaactga cgatgggttg ggaccaaacc acctaagcgt gactggtgtg gacgccagct
     1861 tctttcaaat tatcggtacc gcgttgtatc tgaaagcagg aactgctctc agttctacaa
     1921 tcaagccgag ttacagcgtc accgttaatg tcgacgaccc aacggtcggc agcacgcccg
     1981 atgcaagcgc aaacttcaca ctcacagtta ccgcagctac aggtggaact gcctcactcg
     2041 tcatttccga ggtggcaccc tggtcgagcg gaaatagccc gataagtcta cgcgtggatt
     2101 ggttcgaggt aacgaacacc ggcaacgcga ccgcaaacat cgccggctgg aagatggatg
     2161 atgactcgca ctccttcaac accgccgttg ctttgaacgg catcacgaac atcgcgccgg
     2221 gagagtcggt gattttcctg gagactacag atcttgcagg taaaagcgct gcgtttaaga
     2281 ctctttggtt tggcgctaac ccgcccgcta accttcagat cggcagctat agcggttcgg
     2341 gagttggtct aagcacatcg ggagatcagg tcaatctatt cgatagcggc ggaatccagc
     2401 aggccggcgt gatctttggc gcttctcctt ctggtccatt tccgaccttc gataacgcag
     2461 ctgaattgaa caacgctacc atttcgctcc tgagtgctgc cggaattaat ggtgcattcg
     2521 tcgctccaaa tgacagcacc gaaacagggt ctccgggaac tataggagca ccttcagcac
     2581 cagtcgtcgg gattacagcc acagatccta gcgcgaccga gaccggaaac gacagtgcta
     2641 cttttcgcat cactcgtaac ggaagcaatg ttggtccgtt aactgtcagc tacacgatcg
     2701 caaccggagc cggacaagcc accagcgccg attactctcc agcgctgact ggcgtcgcga
     2761 cgatcgcttc tggacagtcg tttgtcgaca tcatcattac gccagtaact gacaatctgg
     2821 tggaaggcag tgagacggta acacttacgc tgggtgattc gggtagttac gatgtcggtg
     2881 cgaataaaac cgcaaccgtt accattactg acaacccgtt cctcggagtc gccgcgggtg
     2941 atgcaaatgc gagcagcgcg gtgttgtgga ccaggatcaa ccgagcgcag agcgcgacgg
     3001 tgacagcaca ggtatcaacg gatgcggcct tcagtgcagc gtctttaaca ttctcaggca
     3061 caaccgatcc gacaaaagat aatacgttga aaattgtggc gaccgggctg gcgtccggca
     3121 cgcgctacta ttaccggttc ataatcgatg gcacgggcga aaccagtcgt actggcacct
     3181 ttaagaccgc gcctctcgcg aacacggcgg tgccattgca cttcgcgttt agcggcgata
     3241 acgatggtct gatgcgtcct tatgcgcttg ctaacgtcat tccgtcgcag cacctcgact
     3301 tctatcacaa ccttggtgac gtaatttatg agactgcaag taatcttact
//
LOCUS       DASSFP010000131         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1966532, whole genome shotgun sequence.
ACCESSION   DASSFP010000131 DASSFP010000000
VERSION     DASSFP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966532"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..2385
                     /gene="hypF"
                     /locus_tag="VFH91_02815"
     CDS             109..2385
                     /gene="hypF"
                     /locus_tag="VFH91_02815"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET6861942.1"
                     /translation="MTIRAQILVRGIVQGVGFRPFVYSHASRMSLTGRVRNNVSGVLI
                     DVEGSALAIQRFVHAIGSNPPPLSQIESIEYKENLPPAGYPDFLILESNLAGDRTLPV
                     SADFGTCNECVRELFDQRNRRYQYPFINCTNCGPRFTIVEDVPYDRARTTMRHFKMCH
                     ECRVEYENPLDRRFHAEPIACATCGPRLFLRDRAKELLLDDLITRSQELLRAGKVVAI
                     KGIGGYHLACDASSDDAVNRLRQRKYREDKPFALMARSLEVVRQHCNVSDVEARVLLS
                     SNRPIVLLERKPACVLPTSIAPNVQTLGFMLPYTPLHYLLVEKIAGPLVMTSANVSDE
                     PICYSDEDARLRLGNIADYFLFHDREINIRTDDSVVRVFNDRKLMVRRSRGYAPAPIR
                     TAFRVTKPILACGAELKSTFCLARDHDSYLSHHIGDLENFETLMSFKEGISHFQRLFG
                     IEPELVAYDLHPDYLSTKYAQSLEGPEKIGVQHHHAHIASCMADNGIDGEVIGVAMDG
                     LGFGLDGKLWGGEFLVADFEVAERVAHLAYVPLAGGAKAVREPWRLAAVYLQLAFGNE
                     CHALKLDFNERLTPKKWSSLRSMVAAKVNSPETSSMGRLFDAMAGLLCLRESVNYEGQ
                     AAIELETIAADEVEGFYEFLIDGKQISALDVIRGAVRDLLDGVSHAEISGKFHLGVAN
                     LILSMAEKIKAERGLNRVALSGGVFQNMLLVKHVCELLGQAGFEVFTHSRVPTNDGGI
                     SLGQAAIANARANSGRCH"
     gene            2386..2652
                     /locus_tag="VFH91_02820"
     CDS             2386..2652
                     /locus_tag="VFH91_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET6861943.1"
                     /translation="MCLAIPGKIIEIVDEENRIAKVEVGGVKRNVNIGMLSEDETHIG
                     DYVLIHVGFAMSKVDEHEAQETLRLLQELGEFENEFAQFKTTVD"
     gene            2660..3355
                     /locus_tag="VFH91_02825"
     CDS             2660..3355
                     /locus_tag="VFH91_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET6861944.1"
                     /translation="MTKPTLDNFSRSFYPFLHEEKGESESLLNDLGFSLLEKARESVE
                     VKSQFFAQNKETILAASLALAQAFNRGRKLLVCGNGGSATDAQHVAVEFMHPITVGRK
                     ALPAICLTNDIAMVTAVANDVGFDDVFTRQIIGLGVEGDVLLGISTSGNSRNLLHGFA
                     TARRLKLLTIGFAGSDGGEMSAMNAAGLLDYCLTVPTASIHRIQETHVTLYHIMWDLV
                     HTMMENGAILKEK"
BASE COUNT          913 a          818 c          977 g          921 t
ORIGIN      
        1 ggccgggaat gaaaactatg gaagtgtcgg ttaagaccgg cgaaggaata atcgaatttc
       61 tggacctgat tgtttcccat gtgaatcaga aagtttctat ctgacaaagt gactatcaga
      121 gcgcaaatac tcgtgcgcgg catcgttcaa ggcgttggct ttcgaccctt cgtctattcg
      181 cacgcgagtc gcatgtcgct cacgggccgg gtacgtaaca acgtaagtgg agtattgatc
      241 gacgttgaag gaagcgcgtt agctattcag cgattcgttc acgcaattgg tagtaatccg
      301 ccacctctct cgcagattga atccattgaa tacaaagaga acttgccgcc ggcaggctat
      361 ccagactttt tgatcctgga gagcaactta gccggtgatc gaacgcttcc cgtctcggca
      421 gacttcggaa cctgcaacga gtgtgtgcga gagcttttcg atcagcgaaa tcgtcgttat
      481 cagtatccat tcatcaactg caccaattgt ggtccgcgtt tcacaatagt cgaagatgtt
      541 ccttacgacc gagcgagaac tacgatgcgt catttcaaga tgtgtcacga gtgccgtgtc
      601 gaatatgaga atccgcttga tcgtaggttt catgcagaac cgatcgcgtg cgcaacctgc
      661 ggtcccagat tatttctacg tgatcgtgca aaggaacttc tcctggatga tctcattacc
      721 cgttcacaag agttattgcg cgcgggcaaa gtcgtggcga tcaagggtat aggtggttac
      781 catctggctt gtgacgcgtc ctccgacgac gctgtaaaca gattaagaca gcgtaagtac
      841 cgcgaggata aaccgtttgc actgatggcc cggtcactcg aggttgtgcg acagcactgc
      901 aacgtttctg atgttgaagc aagagtgctg ctgtccagca atcgaccgat agtgcttctc
      961 gaacgcaaac cagcctgtgt gttgccaacg tcaattgccc caaacgtaca gacactcgga
     1021 ttcatgctgc cttatacgcc gctccattat ttgttagttg agaagatagc tggaccactg
     1081 gtgatgacga gtgccaatgt ttcagacgag ccgatctgtt attcagacga agatgctcgc
     1141 ctgcgcctgg gtaatattgc agactatttt ttgtttcacg accgcgagat caacatccgc
     1201 accgacgact cggttgttcg agtcttcaat gacagaaagt tgatggtaag aagatctcgt
     1261 ggatatgctc ccgcgcccat tcgaacagcc ttccgtgtca ccaaacccat cctcgcctgc
     1321 ggtgcagaac ttaagagtac gttttgtttg gctcgcgacc atgattccta tctcagtcat
     1381 cacatcggag atctggaaaa ctttgaaaca ctcatgtcat tcaaagaggg tatcagtcac
     1441 tttcaacgac tttttgggat cgagccagag ctagtcgcat acgatcttca tcctgattat
     1501 ctttcaacga agtatgcaca atctttggaa ggaccagaga agattggcgt gcaacaccat
     1561 catgcacaca tcgccagctg catggcagat aacggaattg acggcgaagt aattggcgtg
     1621 gcaatggatg gactgggctt tggtctcgat ggaaagctct ggggcggcga atttttggtg
     1681 gctgactttg aggttgccga acgggtcgcg catcttgcct acgtaccact cgccggaggc
     1741 gcgaaagcag ttcgcgaacc gtggcgttta gcagccgtct acttgcagct ggctttcgga
     1801 aacgagtgtc atgcgctcaa gctcgacttc aacgagcgtt tgacacctaa gaagtggtcc
     1861 agcttgcgaa gtatggtggc tgcgaaagta aacagtccgg aaacttccag tatgggtcgt
     1921 ctctttgatg caatggcagg actgttgtgt cttagagagt ccgtgaacta tgaagggcaa
     1981 gcggcgattg aattggaaac catcgcagct gatgaagttg aaggtttcta tgagttcctg
     2041 atcgatggca aacaaatatc cgcgctggat gtgattaggg gagcagttcg cgacttgctg
     2101 gatggggtgt cgcatgccga gatttcaggc aagtttcacc ttggtgttgc caatctaatt
     2161 ttaagtatgg ctgaaaagat aaaggccgag cgtggactga atagggttgc tctgtcggga
     2221 ggagtctttc aaaacatgct gcttgtcaaa catgtctgcg agttactagg acaggccgga
     2281 tttgaagtat tcacacatag tcgtgttcca accaatgatg gcggtatctc gttgggccag
     2341 gcggcgatag caaacgctcg cgcaaattcg gggaggtgcc attgaatgtg tttggcaata
     2401 ccaggaaaaa taattgaaat cgtcgacgaa gagaatcgca tcgccaaggt cgaagtgggc
     2461 ggcgttaaga gaaatgtcaa tatcggtatg ctatccgagg acgagaccca cattggtgac
     2521 tacgtgctga ttcacgtcgg ctttgcaatg agcaaagtgg acgaacatga agcacaggaa
     2581 acgctgcgtt tgttgcaaga gcttggagag tttgaaaacg aattcgcgca attcaagact
     2641 acagtcgatt gatctgaata tgacgaagcc aactctcgat aacttttcgc gttccttcta
     2701 tcccttctta catgaagaga agggagagtc ggagtcatta cttaacgatc tcgggttttc
     2761 tctattagaa aaagcgcgag agagtgtgga agttaagagc caattctttg ctcagaataa
     2821 ggagactatt cttgcagcgt cgctagcgtt ggcccaggcg tttaatcgag ggcgtaaatt
     2881 gctggtgtgc ggcaatggcg gatctgcgac cgatgctcaa catgttgccg ttgagtttat
     2941 gcatccgatc acggttggtc gtaaggcctt gcccgcgatc tgtttgacga acgacatcgc
     3001 tatggtcaca gcagtggcga atgatgtcgg ctttgatgac gtatttactc gccagattat
     3061 cggtcttggg gtcgagggcg acgttctgct tggaatttct acgagtggca attccagaaa
     3121 tctccttcat ggttttgcga ccgcgcgccg gttaaagctc ttgactattg gatttgcggg
     3181 cagtgatggt ggtgagatga gcgccatgaa cgctgcaggt ctgttggatt attgtctaac
     3241 ggtacctact gcgagcattc atcgcattca ggagacacat gtgacgctgt atcacatcat
     3301 gtgggatctg gtccatacga tgatggagaa tggggcgatt ttgaaagaga aataagtccg
     3361 gtcatttttc atttccggat ctcggtcact aactaattct taggccctcg agatccaaaa
     3421 atgaaaaatg ggaaaatgac aaatgagaaa tgaaagatct tctcttaagg gggacttggc
     3481 gcgatgaaat ttatcgacga atacagacaa agtgatctcg ccatcgcgct ggccggaaag
     3541 atcgctagtc aaacctccag gtcactaaag ctgatggagg tctgcggcgg ccacacccat
     3601 acaattttca agtacggcat cgaggatct
//
LOCUS       DASSFP010000132         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_26110, whole genome shotgun sequence.
ACCESSION   DASSFP010000132 DASSFP010000000
VERSION     DASSFP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26110"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..1261
                     /locus_tag="VFH91_02830"
     CDS             428..1261
                     /locus_tag="VFH91_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HET6861945.1"
                     /translation="MAATLTQERFTGPDWVFERKFDGIRLMTYKNGSVVNLFSRNQLP
                     QNLPSIAEAIEKLPAKQLILDGEVTWGKGSSAYHIFDILWLDDRSLTSLSYEERRSIL
                     ESLPLSPPLELVTQLTDAVPWDRALAEGWEGVIAKRRDSKYEHRRSKTWLKMKCELAQ
                     ELVVGGFTDPQGQRVGLGALLVGYYDNEDFVFAGKIGTGFDTKLLLELRAKLDAIEIE
                     KPPFTRAIGLPRLRAHWVKPEIVVQVGFIEWTTNDKLRHPRLLGVRTDKPARDVVKET
                     A"
     gene            1258..2139
                     /gene="ligD"
                     /locus_tag="VFH91_02835"
     CDS             1258..2139
                     /gene="ligD"
                     /locus_tag="VFH91_02835"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6861946.1"
                     /translation="MITHPEKVLFPGDGITKAELAGYYEFIAPIILPHIRNRPVTMER
                     YHRGIGEAGFFQKDVSKGFPEWLKRVEVPKHGGTVHHPLVTDTRSLVWLANQNSITPH
                     VWTSRVPHLYQPDLCVFDLDPATDTEVERVCAASLKLRDLLSELGLPSWVKTSGSKGF
                     HIVVPLDRKSDFGTVFRFANRVGTILVNRDPVNLTQEFSKSDRGGRILVDTGRNGYSA
                     TFACAYAVRAKPAAPVSAPCSWEEVESMTVAPRTFTLRNMPERIAQVGDLWSDMLRRR
                     RSLTKALLECGGPAPLC"
BASE COUNT          570 a          566 c          574 g          545 t
ORIGIN      
        1 ccagatgcca atcacacgca gatctcagga acattccttt tggcatattg ctgcgccaaa
       61 actccattgg tttcccgacg attaggtggt caatgcctag ttgagaagcg cgtgccgcaa
      121 ttgagaggcc gaagggccca gcaccaatga tcaatacgtc cgttttactt tgcaccgtcc
      181 cttagtaaat catgcgctgc ggaaaatctg aaatgtactc gccgcccatg attttgactc
      241 atcgatcatc aatttcagaa gcactcgaat cattatctga taagttaaac ttgcgcacga
      301 taatcaggag cttttcagtg attcgagcaa tagaattcaa ctacagggct ttgcacaact
      361 acagctcctg ataatgaacg ataaaggaac ccagagaatg ccgtcattcc ccgattggct
      421 catacctatg gctgcgaccc taacgcaaga acgcttcacc gggcccgact gggtgtttga
      481 acgtaaattc gacgggatac gcttaatgac ctacaagaat ggtagcgttg ttaatctctt
      541 ctcacgcaat cagctgccgc agaaccttcc ctcaatagct gaagcgattg agaaattacc
      601 tgctaaacaa ctcatactcg atggagaggt cacctggggt aaaggttcga gcgcttatca
      661 catcttcgat atcctgtggt tggacgatcg aagcttgacg tcgctttcgt atgaagaacg
      721 ccggtcaatc ttagagtcgt tgcctctttc gcctccgctc gagctggtaa cacagcttac
      781 tgatgcggtg ccctgggatc gagcgctcgc tgaaggatgg gaaggagtca tagcgaaacg
      841 gcgcgattct aaatatgagc atcgtcgctc gaaaacatgg ctaaagatga aatgtgagtt
      901 ggcccaggaa ctggtcgtcg gaggttttac ggatccacaa ggccagcgtg taggtctcgg
      961 tgcactactt gtcggttatt acgataacga ggattttgtc ttcgcaggga agattggaac
     1021 gggctttgac actaaactac ttctggaact cagagcaaag ttggatgcta tcgaaattga
     1081 aaagcctcca ttcactcgtg ccattggtct acccaggctc cgcgcgcatt gggtcaagcc
     1141 tgaaatcgta gttcaagtcg gctttatcga gtggaccaca aacgataagt tgcgccatcc
     1201 tcgtctcctc ggcgtgcgga cggacaagcc agcgagagat gtcgtaaagg agactgcttg
     1261 ataacgcacc ccgagaaagt tctatttccc ggcgacggca taaccaaagc ggagctcgcg
     1321 ggttattacg aattcatcgc tccgatcatc ctgcctcata ttcgaaaccg gccagtcacg
     1381 atggagcgct accatagagg gattggtgaa gccggcttct ttcagaaaga cgtctcaaaa
     1441 ggatttcccg aatggctgaa acgagtggag gtaccaaaac acggaggaac ggtccatcat
     1501 cctttagtta cagacactcg ctcacttgtc tggttagcga atcagaacag cattacccca
     1561 catgtctgga cgtcgagagt tccacatctt tatcaaccgg acctttgtgt gtttgatctg
     1621 gatcccgcta ccgacaccga agtcgaacgg gtgtgtgctg cgtctctgaa gctgcgagac
     1681 ttattgtcgg aattaggtct gccaagttgg gtgaaaactt ctggttcgaa aggctttcac
     1741 atcgttgttc cgttggacag aaaatctgac ttcggaactg tctttcgatt tgcgaatcga
     1801 gtcgggacga ttttagtaaa tagagatcct gtcaatctta cccaggagtt cagcaagtca
     1861 gaccgcggcg gtcgtattct ggtcgatacc ggcaggaatg gatacagcgc aaccttcgct
     1921 tgtgcttacg cagtgagagc taaacctgcg gcgccagtat ctgctccatg cagctgggaa
     1981 gaggtggaaa gcatgaccgt cgctcctcgc acttttactc tccgcaacat gcctgagcgg
     2041 atcgcacaag tcggtgattt atggtccgac atgctgcgaa gacgtcgctc tctaacgaag
     2101 gcacttttgg agtgcggcgg cccggcgccg ctttgttaaa tcagcacgcc ggttgttcaa
     2161 caaagcgccg gggccgccgc actccaaagc gtaaccaatc ctgtgtatcc ggtgtctata
     2221 taagtgcgga agaacaagga ggtttttcga aaaac
//
LOCUS       DASSFP010000133         4316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1967656, whole genome shotgun sequence.
ACCESSION   DASSFP010000133 DASSFP010000000
VERSION     DASSFP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967656"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1686
                     /gene="fumC"
                     /locus_tag="VFH91_02840"
     CDS             277..1686
                     /gene="fumC"
                     /locus_tag="VFH91_02840"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET6861947.1"
                     /translation="MSQQLTEPTRKTRIETDSMGEIEVASDKYWGAQTQRSLHHFNIG
                     NDRMPREMIRALGVLKKAAALANQQLGKLSPDKTSLIVQACDEVIDGKLDEHFPLRVW
                     QTGSGTQTNMNANEVISNRAIEIAGGVMGSKKPIHPNDDVNMSQSSNDTFPTAMYIAA
                     AEELSKLIPRITELRDAIDAKAQEFAGVVKIGRTHLQDATPLTVDQEMGGWASLVDRD
                     VERLKLALPGLFDLAIGGTAVGTGLNSHPEFGNEAAKKISEITGLPFKSHPNKFAALS
                     AHDELVFASGSLKTLAGSLMKIANDVRWLASGPRCGLGELILPENEPGSSIMPGKVNP
                     TQSEAMTMVAVQVFANDLATSFGGSQGNFELNVFKPVMIHNFLHSVRLLNDATRSFID
                     FCISGIEINRDQIDEHLKGSLMLVTALNPHIGYDNAAKVAKNAHKKKISLRESAIELG
                     LLSGEDFDRLVKPEEMTHP"
     gene            1765..1995
                     /locus_tag="VFH91_02845"
     CDS             1765..1995
                     /locus_tag="VFH91_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6861948.1"
                     /translation="MPDKTDKVKENVAGSWYVDSSCIDCDVCRDTAPDNFVRSDENNY
                     SFVCKQPETAEEKVLCEEAMSCCPVEAIGNDG"
     gene            2302..3723
                     /locus_tag="VFH91_02850"
     CDS             2302..3723
                     /locus_tag="VFH91_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET6861949.1"
                     /translation="MLFATGKYWIFLTLVFFGYWTVARSRRASVIFLLVASYYFYALW
                     NIKFVPLLLLISTIDFLTARFIAGSNRARIRKVLLSVSIASDIGTLFIFKYFNFFSGS
                     INHLLGQLHLQTRSVLIQDLVIPLGLSFVTFRSLSYVIDVYRKTTPATDRYLDYLAFV
                     SFFPTLIAGPVVRARELLPQLGRYNFESKQALDGIFFILLGLVKKVAIADVLASNLVD
                     RVFDQPQLYSSVETLAAIYGYALQIYCDFSGYSDIAIGSALLLGIKLPENFYSPYRAQ
                     NLREFWKRWHITLSTWLFDYVYISIGGLRKRRFNLYRNLVLTFLVGGLWHGAAWTFLV
                     WGGLHGVGLSINHWWNSFRKQSSSTTTRSRWRKAVCVLATFHFVCLTWVVFRAENLQQ
                     VLEVLQRLTAFQFSVGNLPASLALLLMVAYLSHWLPVRLFVAIKEGWNWLPSPAQAVV
                     VLSTAFGLYYVSGAEVQFIYGNF"
     gene            3710..>4316
                     /locus_tag="VFH91_02855"
     CDS             3710..>4316
                     /locus_tag="VFH91_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861950.1"
                     /translation="MAIFEKLIPGIHKANDALPYPNSILLTKPGRTLLLVLLLALAPL
                     VSPPLERKLSLKGQSYREMLPDAREFIAFKRQSVPALASSFETVDAPVAIKPAAGVCN
                     DQLIEDPEHTLDAFNAHLLQTEQRKAGAITRIVHYGDSPITNDGITGTVRDLLQKRFG
                     DSGHGFILVDRPWDWYGHQAIAFQTGGGWSSASLMTPRSSDG"
BASE COUNT         1047 a         1046 c         1083 g         1140 t
ORIGIN      
        1 ctgtgaactc tttgcaccac gtacagtagt tgagcgagaa accacgtcag gggcaccgaa
       61 ggatgctcgt caggcgttcg caaaactctt taagaagtga aatacaatcc acagattacg
      121 ccgattacac agattctttg ctagaacgcc tttgtgccgt gcctttctct gaagaaatct
      181 gggtaatcgc cataatctgt ggatgatctt ttctttgagg gacctgattc tgcacttgcc
      241 aatcggtgcg tagtcagcca agatctcatc ctggctatgt cccaacaatt gactgaaccg
      301 acacgcaaaa caagaataga aaccgactcg atgggcgaga ttgaagtagc ctcagacaag
      361 tactggggtg ctcaaactca acgctcattg caccacttca acatcggcaa cgatcgcatg
      421 ccccgcgaaa tgatccgcgc tctgggtgtg ctcaagaaag ctgctgccct cgctaatcag
      481 caactcggaa aactttcacc cgataagaca tcgttgatag tgcaggcatg tgatgaagtg
      541 atcgacggaa aactggatga gcattttccg ctgagagtct ggcaaaccgg cagcggtacc
      601 caaacaaaca tgaacgccaa tgaggtcatc tcgaatcgcg caattgagat cgccggtggc
      661 gttatgggta gcaagaaacc aattcatcct aacgatgacg tcaacatgtc gcagtcatca
      721 aacgatacgt ttccgaccgc tatgtacatt gcggccgcag aggaattgag taagttgatt
      781 ccgcgtatca cggagttacg agatgcaatc gatgccaagg cacaagaatt tgcaggtgtc
      841 gttaagattg gtcgcactca tttacaggat gcgaccccgt tgacggttga tcaggagatg
      901 ggtggttggg ccagcctggt cgatcgtgat gttgagcgac tgaagctcgc attacccggc
      961 ctatttgatc tggcgattgg cggcactgca gttggcacgg gtttaaactc ccatccagag
     1021 tttggaaacg aagcggcaaa gaaaatttcg gagattaccg gtttaccgtt taagtctcat
     1081 ccgaataagt tcgctgcttt gtcggcacac gatgaactgg tttttgcgag cggctcgttg
     1141 aagaccctcg caggttcgtt gatgaagatc gcaaatgatg tgcggtggtt ggcttcaggc
     1201 ccaagatgtg gtctgggaga gttgatactg cctgaaaacg agccgggatc gtcgatcatg
     1261 ccgggcaaag tgaatccgac ccaaagcgag gctatgacca tggtggcggt gcaggttttc
     1321 gcgaatgacc tcgctaccag ttttggtggt tcacagggca attttgaact caatgttttt
     1381 aagcccgtga tgatccataa ttttctacat tcggttagac tactaaacga tgctacgagg
     1441 agttttatcg acttctgcat ttcaggcatt gaaattaacc gcgatcagat cgacgagcat
     1501 ctcaaaggct cgctcatgtt agtaaccgct ttgaatccgc atatcggcta tgacaacgcc
     1561 gccaaggtgg ctaaaaacgc tcacaagaag aagattagtt tgcgcgagtc ggccattgag
     1621 cttggtttac tctctggcga agacttcgac aggttggtca agcccgagga aatgactcac
     1681 ccgtaagaga tcgctgcctt aatattttct gaaggacggt agcgaacgtc cttgcgagat
     1741 attttgatta ggagtgaaga gtagttgccc gataagacgg ataaagtgaa agagaacgtg
     1801 gcgggttcct ggtatgtgga ctcgtcgtgt atcgattgtg acgtgtgccg cgatacagct
     1861 cctgacaact tcgtcaggtc cgacgaaaac aattactcct ttgtttgcaa gcagcctgaa
     1921 acggcagaag agaaggtact ttgcgaggaa gcgatgagtt gttgtcccgt ggaagcgatt
     1981 ggcaacgatg gatgaagtta ttggagaatt atgaattgat atttgagaat tgctaattgg
     2041 gttttgtgga gcgcgtccgg atcgaaatag aaattctcaa ataccaaatg ataattctcc
     2101 aatgattttc ttctggcgac taacccctga tccctgacct ctaatccctg tcctgtggca
     2161 aaccacagtc gccacaagcg aacacacgca tcactttcct atggttgtcc ttccctcctg
     2221 acgttggtcc aacgctaaga aacgaaaacc ttcaagagca aaatcagttc tacgttcttc
     2281 attggaagac gcagaaccga catgctattc gctactggca aatactggat ctttctaacg
     2341 ctcgtattct tcggctactg gaccgtagcc agaagtcgcc gcgcctcagt tatctttctt
     2401 ttggtcgcga gttattactt ctacgccctt tggaacatca agtttgttcc actgctactg
     2461 ctcatctcga caatcgactt tttgaccgca aggttcattg ccgggagtaa tcgagcccgg
     2521 atacgcaaag ttctgctgtc agtcagcata gcgtcagata tcggaactct tttcattttc
     2581 aaatacttca atttcttcag tggctccatc aaccatctgc tcggacaact ccacctgcaa
     2641 acgaggtccg tcctcatcca ggacctcgtc attcctttgg gcttgtcttt tgtgacgttt
     2701 cgatcgttga gctacgtgat tgatgtttat cgaaaaacca caccggccac cgaccgttat
     2761 ctggattacc tcgcctttgt ttctttcttc ccaacattaa ttgctggtcc ggtggttcgc
     2821 gcacgtgaat tgttgccgca actgggtcgt tacaacttcg aaagcaagca ggcgcttgat
     2881 gggatctttt tcatcttgct gggactcgta aaaaaagttg cgattgcaga tgtgctcgcg
     2941 tcgaatctgg ttgaccgagt ctttgaccaa ccgcagcttt actcatccgt cgaaacactg
     3001 gcggcgattt atggctacgc tttgcagatt tactgcgact tttcaggcta tagcgacata
     3061 gcgattggat cggccttact tctgggaatt aaactccccg aaaactttta ctcaccctac
     3121 cgcgcacaaa acctgcggga attctggaag cgttggcata tcacgctttc gacctggctg
     3181 tttgattatg tctacatttc gatcggtgga cttcggaaaa gacgtttcaa tctttatcgc
     3241 aatctcgtct tgaccttttt ggttggtgga ctctggcacg gtgcagcctg gacgtttttg
     3301 gtttggggag gactgcatgg tgttggactt tcaattaacc attggtggaa ctcttttaga
     3361 aagcagtcaa gctcaacaac tactcgaagc cgttggagaa aagccgtttg cgttctggcg
     3421 acattccact ttgtatgcct cacgtgggtt gtattcagag ccgaaaatct tcaacaggtt
     3481 ctggaggtgt tgcaacgcct gactgctttc caattttcag tcggaaacct gcctgcatcg
     3541 ctcgcacttc tactgatggt cgcgtacctg agtcattggt taccggtccg tttgttcgtt
     3601 gcgattaagg aaggctggaa ctggttacct tcgccggcac aggcggtggt agtcctttcg
     3661 acagcatttg gcttgtatta cgtctcaggt gcggaggtgc aattcatcta tggcaatttt
     3721 tgagaagttg attccgggca ttcataaagc taacgatgcg ttgccgtatc caaattctat
     3781 tctcctgact aaaccaggtc ggacgctgtt gctggtttta ctactcgcac tcgcaccatt
     3841 agtgtcgccg ccgctcgagc gtaaactttc actaaaaggg cagagctatc gcgagatgtt
     3901 gccagatgct cgggagttca tcgccttcaa gcgccaatcc gttccagcgc tagcatcatc
     3961 atttgaaacg gtagacgctc ccgttgcgat aaagcctgcg gccggagtct gtaacgatca
     4021 gctgattgaa gatcctgaac acacactcga cgctttcaac gcgcaccttt tgcaaaccga
     4081 gcaacggaaa gcgggtgcga tcacgcgaat agttcattac ggagattcac caatcaccaa
     4141 cgatggaatc accggaacag tcagagatct gttgcagaag aggtttggcg actcgggcca
     4201 cggattcatt ctggtggatc gtccatggga ttggtatggc catcaggcga ttgcgtttca
     4261 aaccgggggt ggctggtcca gcgcatcgct gatgacgccg cgttccagtg acggcc
//
LOCUS       DASSFP010000134         6680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2115122, whole genome shotgun sequence.
ACCESSION   DASSFP010000134 DASSFP010000000
VERSION     DASSFP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115122"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFH91_02860"
     CDS             complement(<1..379)
                     /locus_tag="VFH91_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861951.1"
                     /translation="MVQVIKWPGVASHRVTNYSLLLIVLCCFAPTSPLAAAQTNQDVP
                     TLTPNNAVERELKGGETHTYQIHLSSGQFLDLIVDQLGIDVAVNLIGPEGNQVFAIDG
                     PNGRYGPESVAVVAEKSGDYRLEV"
     gene            complement(480..779)
                     /locus_tag="VFH91_02865"
     CDS             complement(480..779)
                     /locus_tag="VFH91_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861952.1"
                     /translation="MGNYNTTSGFNDLIGSMVTNGDDVESYYSNARGNGTVTNLGGSG
                     LHFKVSFTGVSRPYNINATSNSNGFSGNANNNSAAESEEVWTATASTAAPATAYK"
     gene            complement(1178..3820)
                     /locus_tag="VFH91_02870"
     CDS             complement(1178..3820)
                     /locus_tag="VFH91_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6861953.1"
                     /translation="MSETISPNTTLAHYTVLCKIGAGGMGEVYRARDEKLNRDVAIKV
                     LPSEFAKDEERLVRFKREAQVLASLNHPNIAAIYGLEDFDGAVALVMEFIDGSTLADR
                     LERGPIAFEDAHAIAKQIADALEAAHERGIVHRDLKPANVKVTPEGIVKVLDFGLAKI
                     LEGESGVTDLSHSPTLIKGTQAGVILGTAAYMSPEQAKGKVVDKRSDVWAFGCVLFEM
                     LTGRQSFIGETLTDILAAVVRAEPDWNSLPQETPREIRRLLIRCLSKDPRQRLRDIGE
                     ARIAIEESLQYKADEPAGVPAAMPQRPRVRTWQVAIAVAALTIAISSLVMLKLARQGF
                     LVPDVKANSAGTYVVASIGVNEPSLVYLTDRFAVAPDGSSIVFVKQGEGLFTRKRNQI
                     VETLLPGSPKDAYAPAFSPDGKWIAFSSENSLKKIPAGGGAPEVLSRTTDYVVNLTWG
                     SDDVIRFPSKDWDAIRYVSSKGGQLQSLSINKGTKVSRAEWLPGNKLLVSLTDSEGDY
                     VGIRETDGSISRMFEGADAKLTPTNDVLYAKRDGSKWSLMTRSFDPGSPTISGDETLV
                     AQNIAMRYATPAAATAAGDVFFVSGEVKSDRRIVILFRDGSERVLEGAGGPWQELRLS
                     PDGSRLALSRWDGARRTIWTLALETRALTQVTYLDDVFGPVWVGLSNEMLVTQFPRNP
                     GMEGTTMWRVATDGSGGIKPLFQHPESYASSTSQDGRIVYYTSYELNDAAGDLFTLDL
                     SQSPARRTTLLATPADEDHPLPSPNAKWLAYTTNASGSNQVRLTSLGSTKNSTQITMR
                     GGTAIRWTKDSSKLYYRDGDAISVIDLAADGPRLQSRKTAFPLPGDTGTSADVFPDGE
                     KAIVVRGGLMYSDLIVVQGFLPHR"
     gene            complement(3798..4130)
                     /locus_tag="VFH91_02875"
     CDS             complement(3798..4130)
                     /locus_tag="VFH91_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861954.1"
                     /translation="MSETISPNTTLAHYSIVSKIGAGGMGEVYRARDSRLDRQFAIKI
                     LTKTLGNDADRLKRLSRKLAPLQRSTIQTFSSSTTLVDTTLHLTSLRSTDQRPKRQGI
                     NLERNDIS"
     gene            4626..5366
                     /locus_tag="VFH91_02880"
     CDS             4626..5366
                     /locus_tag="VFH91_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6861955.1"
                     /translation="MKSAHLERDRLRPKRPYRIYKREQERISRRNLYRTTTLYSGYSI
                     ILFVLAFRMNHPYRAITYFPVGIPLWMFVEYFSHRFILHRHFKVSKKWYKKFFTRLAN
                     KYLDPLHFGHHERPFDGNHMSGEVRDLLPLLLVFGPLTILLFPSFTASMVIAGTFQSY
                     IAEEWIHHATHYYNFHDPYFRYMKKHHFYHHTSHGMTRGFGTSSGILDVIFRTRYPNN
                     VRQRLYGRGQSQIPNPSEPPNQQSKLEA"
     gene            5363..6145
                     /locus_tag="VFH91_02885"
     CDS             5363..6145
                     /locus_tag="VFH91_02885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6861956.1"
                     /translation="MTQPGYALVTGASRGLGKYFAAALARRKQNLVLVARSNEMLEKV
                     AEKLRSAHGVSVETISIDLARSNAGRDLARELRERNLVIDLLVNNAGFGDHGEFVKLS
                     LERQLEMIRLQNSTAVELTHELLPSMIEQRRGGIINVSSMAGFQPIPYATIYSATKSF
                     LTTFSLALEAEVRRYKLAVVTICPGRLRADPEDEELERRKVPGGEQSHTEVVRAALRI
                     LDQGGGLVIPGAVNKFAAFAERFIPRSRVARLIAKMTKPGRS"
     gene            complement(6496..>6680)
                     /locus_tag="VFH91_02890"
     CDS             complement(6496..>6680)
                     /locus_tag="VFH91_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861957.1"
                     /translation="LCVRATSEIDGNSQRLSEPQDYQLRFEQNAGVPGRAPCEAIVPQ
                     DMYINSTDILHKYTSG"
BASE COUNT         1576 a         1783 c         1652 g         1669 t
ORIGIN      
        1 gcacttctaa ccgatagtct cctgactttt ccgcaaccac tgctactgat tcaggtccat
       61 acctgccgtt gggaccatcg atggcaaaga cttgatttcc ctctggtcca atgaggttta
      121 ccgcaacgtc aattcccaac tgatcgacta tcagatccaa aaactgtcct gaactcagat
      181 gaatttggta agtgtgagtc tcaccacctt tcaactcccg ctcgacagcg ttgttgggag
      241 ttagagttgg cacatcctgg ttggtctgcg ccgctgccaa tggactggtt ggagcaaaac
      301 agcagagaac aatgagcagc agtgagtagt tggtaacacg gtggctggca acaccaggcc
      361 acttgatcac ttgaaccata ggaacctgct ctgcattaga tttccaatat cagatggagc
      421 aacaggtgcc tcgccagctt tgttggtgag gcacctgttc tctgttcaac ccgcgcttgc
      481 tatttgtacg cggttgccgg ggcagccgtg gaggccgtgg cggtccatac ttcttcgctt
      541 tccgcagcgc tattgttatt agcatttcca ctaaaaccat tcgaatttga agtggcgttg
      601 atgttgtacg gtcgcgatac tccagtgaag ctgaccttga aatgaaggcc gctgccgccg
      661 agatttgtta ccgtaccgtt gcctctggca ttgctgtagt agctttcgac gtcatcgccg
      721 ttagtgacca ttgagccaat tagatcgttg aaacctgaag tggtgttgta gtttcccata
      781 tcgtctcctt tttttggtag ctgggattaa aagccaaaat tcattggctg tgtaaagggt
      841 tggcggcttt catatctggg cagatatcgc aagcttcgcc aaatcgcggg gacgcaagat
      901 agttgcgcct gagccttgat ctcttacgaa gttacagtgg tgttcccaat tccttatgcg
      961 agaaccagct taggaaacca ccatgatcga aggcgacagg agccgccgcg gaaagccgtt
     1021 cggataaagc ttagaaagag tcagtctcgc aaagaccgac aaacctgtga attgcagtgg
     1081 cgcacatgat attcccccga cctaatgtgg tcaatggatc cagtggtggt ctgtgctctc
     1141 ttctgcgggg caagtcacta ataaacccca ggaacgctca acggtgcgga agaaagccct
     1201 ggaccacaat caaatccgag tacatcagtc cacctcttac tactattgct ttttcgccgt
     1261 ccgggaaaac gtcagcagac gttccggtgt cccctggcaa tgggaaagcg gttttccttg
     1321 attgcagtcg cggaccatct gccgctaaat caatcaccga gatcgcatca ccatcgcggt
     1381 aatatagttt cgacgaatcc ttggtccatc gaattgcggt accaccacgc atcgtgatct
     1441 gagtggagtt tttcgtgcta ccgagtgagg tgagtctcac ttgattggaa ccggacgcat
     1501 tagtggtgta ggccagccat ttcgcgttcg gagacggcaa tggatgatct tcgtcagcag
     1561 gcgttgccaa aagagtcgtg cgtctcgcgg gggactgact taggtccaac gtaaataagt
     1621 ctcccgcagc atcgttgagt tcgtacgatg tgtaataaac gatgcggcca tcctgcgaag
     1681 tggacgacgc gtacgattcg ggatgctgga agagaggctt aatcccacct gaaccatccg
     1741 tcgcaacccg ccacatggtc gttccttcca tgcccgggtt tctcgggaac tgcgtaacaa
     1801 gcatctcatt ggataaccca acccagaccg ggccgaacac atcgtccaga taggttacct
     1861 gagtgagtgc tctcgtttcg agagcaagcg tccagatagt gcgtcgcgct ccgtcccaac
     1921 gactaagagc aagccggctt ccgtcaggcg atagtcgcaa ttcctgccat ggccctccag
     1981 cgccttcgag tacacgttcc gagccgtcac gaaaaagaat tacgattcgt cgatcactct
     2041 tgacctcacc tgaaacgaaa aaaacgtctc cggcggcagt tgctgctgcc ggtgtggcat
     2101 accgcatagc aatattttga gcaaccagcg tttcatctcc actaatcgtc ggcgagccag
     2161 gatcgaagga tcttgtcatc aacgaccatt ttgaaccgtc cctttttgcg tataacacat
     2221 cattcgtcgg agtgagcttt gcatcggccc cctcaaacat gcggcttatt gaaccatcgg
     2281 tttcgcggat gccaacgtaa tcgccctccg aatccgtaag cgaaacaagt aatttgtttc
     2341 caggaagcca ctccgccctg ctgaccttag ttcctttatt aatgctgaga gattgcaact
     2401 gcccgccttt cgaagacacg tatctgatcg cgtcccagtc ctttgaagga aagcgaataa
     2461 catcgtcgct tccccaggtg agattcacca cgtaatctgt cgttcgggaa agaacttccg
     2521 gcgctcctcc gccggcaggt atctttttca agctgttttc agaactaaat gcaatccact
     2581 ttccatccgg tgaaaacgca ggtgcatacg catcttttgg cgaaccgggc agcagcgttt
     2641 ccacgatctg gttccgcttg cgtgtgaaga gtccttcacc ttgctttaca aaaacgatag
     2701 aactgccatc aggggcaaca gcgaacctgt cagtcagata aaccagacta ggttcgttca
     2761 ccccgatcga agctacaacg tatgttcccg cagaatttgc ttttacatcg ggcacaagga
     2821 aaccctgccg cgcgagcttc agcattacca aacttgagat cgctatggtg agagccgcta
     2881 ccgcaattgc aacctgccac gttcgaactc gtgggcgctg tggcatcgca gcaggaacgc
     2941 ctgcgggctc gtctgctttg tattgaaggg attcttcaat tgcgatgcgc gcttcaccga
     3001 tgtcgcgcaa tcgctgcctg ggatctttac ttaagcaccg gattagtaat cgtcgaattt
     3061 cgcggggcgt ctcttgagga agcgaattcc aatccggctc tgcgcgcaca acggcagcca
     3121 gaatgtcggt tagtgtctcc ccgataaagc tctgtctgcc ggtaagcatc tcaaacagca
     3181 cacagccaaa ggcccacaca tccgatcgtt tgtcgaccac tttacctttg gcctgttccg
     3241 gactcatata cgcagccgtg ccaagaatta ctcctgcctg cgtgcctttg atcagggtgg
     3301 gcgagtgtga aagatcagtg actccggatt cgccttccaa tatttttgca agaccgaaat
     3361 ccagtacttt tacaattcct tccggagtta ctttcacatt tgcaggtttc agatcacgat
     3421 ggacgatgcc gcgttcgtgt gcggcttcga gggcatccgc aatctgtttt gcaattgcat
     3481 gcgcatcctc gaaggcaata gggccacgtt caaggcgatc tgcgagagtt gatccatcga
     3541 tgaattccat cactagtgca acagcaccgt cgaaatcttc gaggccataa attgctgcaa
     3601 tgttcggatg attcaaagag gcgagcacct gcgcttcacg tttgaaacga actaagcgtt
     3661 cttcgtcttt agcaaactcg gatggcaaaa ccttgatcgc gacgtcacga ttcaacttct
     3721 catcacgcgc acgatagacc tcacccattc cgcctgcgcc aatcttgcag agtactgtgt
     3781 aatgcgcgag agtcgtgtta ggagatatcg tttcgctcaa ggttgatgcc ctgacgtttt
     3841 ggtctttggt ccgttgatcg caacgatgta aggtgaaggg tcgtgtctac caacgtcgta
     3901 gacgatgaga atgtttggat ggttgagcgc tgaagtggcg cgagcttcct gctcaatctc
     3961 ttcagacgat cggcatcatt cccaagggtt ttcgtgagaa tcttaatcgc gaattgtcga
     4021 tcgagcctgg aatcgcgagc tcggtagact tctcccatac caccggcgcc gattttggag
     4081 acgatagagt aatgtgcgag cgtcgtgttg ggggaaatcg tttcgctcaa actttacttc
     4141 aattgtggtc cgcgacgaac caaggttagg gcattggcgt gtcagacgta tggactcgcc
     4201 agacaaaggg atttatctaa gagagcgtca ggactcaaca taaaatacct gcttgaagtg
     4261 gaggcattct aaaacactct ggtcacttca cctagcgtaa agttcagaca ctgtaaaggt
     4321 cacctgagaa aacgtcgtga cgctcaaccg cgaaaactaa aagcagagaa gtaggaaatt
     4381 tttcatgttg gttcttctca gttgtaaatc gcgttgaaga gttttcggca agctgggctg
     4441 gttcgccctc gggatggccg ctcccatgca atttgagaat cttgtattgt ttcccgtgct
     4501 gatttcttgt tgcctggaag ctctctttgg cggcgtttct tctcgaagat tatgagttga
     4561 ctaaaaaccg tgattcgggt caaaattcgg actgtctggt aattattgat tgaagccttc
     4621 tcgacgtgaa atccgcgcac ttagaacgtg accgccttcg gcctaaacgg ccctaccgaa
     4681 tctacaagcg tgagcaagaa cgcatctcgc ggcgtaatct ctaccgcacc accacactct
     4741 atagcggata ttcgataatc ctttttgttc tggcatttcg aatgaatcat ccgtatcggg
     4801 ccataaccta cttccccgtc ggaatcccac tttggatgtt cgtggagtat ttttcacatc
     4861 gttttattct gcatcggcat tttaaagtgt cgaagaagtg gtacaagaag tttttcacgc
     4921 gcctggcgaa taagtatctt gacccacttc atttcggaca tcacgaacga ccctttgatg
     4981 gcaatcacat gagcggcgag gtacgcgatc tgttgccact ccttttggtg tttggaccct
     5041 tgaccattct tctctttccg tctttcacag cgtcaatggt gatcgccggg actttccaat
     5101 cctacatagc tgaagagtgg atccatcacg cgacgcacta ctacaatttc cacgatccct
     5161 acttccgcta catgaagaaa caccatttct atcaccacac gtcccatggg atgactagag
     5221 gctttggcac gagcagcgga atattggacg tgattttcag aactcgatat cccaataacg
     5281 tacgccagcg tctgtacggc cgcgggcagt cccaaatccc caatccaagc gaaccgccaa
     5341 accaacaatc caagctggaa gcatgacaca gccgggctat gcccttgtca ccggagcctc
     5401 gcgcgggtta ggcaagtact tcgccgccgc gttggcccga cgcaaacaga acctggtact
     5461 ggttgcacgc tcaaatgaaa tgctggaaaa ggttgccgaa aagctaaggt cggctcacgg
     5521 agtctccgtt gagacgatct cgatcgacct ggcccgcagc aatgccggcc gagatcttgc
     5581 gcgagagttg agagagcgca atctcgtgat cgatctgctg gtaaacaatg ccggcttcgg
     5641 tgaccatggt gagttcgtca agctttcgct cgagcgacaa ttggagatga tccgcttaca
     5701 gaactcaacg gcagttgaac taacgcatga gctacttccg tcgatgatcg aacaacgccg
     5761 cggtggcatc atcaacgttt cctccatggc cggctttcaa ccgattcctt atgccacgat
     5821 ttattccgcg acgaaatctt ttctaaccac gttctctttg gctctggaag ctgaggtacg
     5881 ccgatacaaa ttagccgtag tcacaatttg ccccggacgg ctacgcgctg atcccgaaga
     5941 tgaggaactg gagcgaagaa aagtccccgg cggcgaacaa tctcacacgg aagtcgtccg
     6001 agctgctctg cgaatcctcg accagggcgg aggtctcgta attccaggcg ccgtcaataa
     6061 atttgcggcc tttgccgaac gatttattcc gcgaagtaga gtagctcggc tcattgccaa
     6121 gatgacaaag ccggggcgtt cttagcgtga ggcaactggc caggcctggc cgagccttgg
     6181 cactcagcgc tccgcgagac tctcgcttat cgatgaatcc ttgcatgcgc tagagccgga
     6241 ccgcgatcac cttgtcctct tcttcgagag taactgcacc gcgatcttcg acggcgaagc
     6301 cgcatggccc taaccgaccg ttagtagatg tttcgttctt atgtatgact cattcctccc
     6361 taaaaatatt aacgtcaatt aaccagtcgc gtgcgcctga aaactccatc ggatacttat
     6421 ggccaatttt ttgtctgagt cgtagcgcta tgacacttga ccctgatttg agtggccgtt
     6481 atccctaaca ccaggttaac cagaggtata tttgtgcaag atgtccgtgg aattgatata
     6541 catatcttgc gggacgatag cctcacacgg agcgcgtcca ggaacacctg cattctgctc
     6601 aaagcgcagc tgataatctt gtggttcact aagccgttgg gaattgccat caatctccga
     6661 agttgccctg acgcataagt
//
LOCUS       DASSFP010000135         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1969440, whole genome shotgun sequence.
ACCESSION   DASSFP010000135 DASSFP010000000
VERSION     DASSFP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969440"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFH91_02895"
     CDS             <1..192
                     /locus_tag="VFH91_02895"
                     /inference="COORDINATES: protein motif:HMM:NF025021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6861958.1"
                     /translation="NAVKRTAALHHAAMSGDVEMAKLLIELGADPLVRDAEFNAFPRG
                     WAEFGEKREVAEFLRQFEE"
     gene            376..1110
                     /locus_tag="VFH91_02900"
     CDS             376..1110
                     /locus_tag="VFH91_02900"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET6861959.1"
                     /translation="MSVDILDGIKQIRLRESDEYLKKREELRQAEIESLKSRERVAQL
                     RRELPPGPVLQDYEFTEGPGLLGNGDAPIRKVKLSELFTRPDRALVVYQFMFGKKQAN
                     PCPMCTLVIDSLNGVAHHIAENVDLVIVAAADPVALRKHARRQGWDRLRLLSTAEGST
                     FKYDLGGEDREGNQDSTLTVITRDADGTLRHFYTGHPRVAPEIMERGLDLLSPVYNVL
                     DLTPQGRGSWYASLNYSPNVLAAHQH"
     gene            1215..1589
                     /locus_tag="VFH91_02905"
     CDS             1215..1589
                     /locus_tag="VFH91_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6861960.1"
                     /translation="MFENTKAFSGFSVNDLQKAKQFYGKTLGLKVNEREEGLELEVGG
                     GNKVFIYPKPNHTPATFTILNFPVDDIDKAVDQLGKLGVKFEHYDSEPIKTDAKGISR
                     GPHPDIAWFKDPAGNILSVLKP"
     gene            2177..>3931
                     /locus_tag="VFH91_02910"
     CDS             2177..>3931
                     /locus_tag="VFH91_02910"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET6861961.1"
                     /translation="MELSLILLVSLLVVCSAVLIRKKAMIARRVTSIAPSFRSFLAGA
                     GAITFGLLFATPTFAQSHQGGEASLELPDLSQVTFLNGITGHNLLLFGIVISVLGLGF
                     GLAIYMQLQKMPVHRAMKEISELIYETCKTYLITQGKFILILEAFIAVVIILYFGVLS
                     GMEIPRVIIILAFSLVGIAGSYGVAWFGIRVNTFANSRTAFASLEGKPFPLYAIPLKA
                     GMSIGMMLISVELLIMLCILLFIPGSYAGPCFIGFAIGESLGAAALRIAGGIFTKIAD
                     IGSDLMKIVFKIKEDDARNPGVIADCTGDNAGDSVGPSADGFETYGVTGVALITFILL
                     GVSNPRVQVQLLVWIFVMRVMMIVASALSYFVNDAIAKARYKNVDKMNFEAPLTSLVW
                     ITSIVSVVITFIVSKLTIPALSGNENLWWKLAVIISCGTLAGAIIPELVKVFTSTESR
                     HVQEVVVSSKEGGPSLNILSGLVAGNFSAYWLGISIVGLMAIAYFFATMGDASGFSVG
                     SLMVDPVAAPVFGFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSLIEQIPNVEE
                     EVKKDFNINVNFGRAKHLL"
BASE COUNT          958 a          949 c         1005 g         1019 t
ORIGIN      
        1 aacgcggtaa agcgaactgc ggctctgcat catgctgcaa tgtcgggtga cgtcgagatg
       61 gcgaagttac ttatcgaact cggtgcggat ccattagtgc gggatgcgga gttcaatgcc
      121 tttccgcgcg gctgggctga attcggtgag aagcgagaag tcgcggaatt cctgcggcaa
      181 tttgaagagt gatcgcgggt agaacatcgt gtctgtctta tttgcatacc atccgaaagt
      241 tacctcccaa cagtgtcgat ttgaaaccta ttcaaacgac ttactaataa gctcagggtc
      301 tcaacattaa tcgatgcaga ccgcggtgcg gatgttgcag actttctaga gagcattaag
      361 agaaggagat tcaagatgag tgtggatatt ttggatggta ttaaacagat ccgcttgcgt
      421 gagtctgacg agtatttgaa aaaacgcgaa gaacttcgtc aggccgaaat cgagtctttg
      481 aaatctcgcg aacgcgtagc gcagttgcga cgagaactgc cacctggtcc agttctccag
      541 gattatgagt tcaccgaagg cccaggcctg cttggtaacg gcgatgctcc gatccggaag
      601 gttaaactca gcgaattgtt caccaggccg gaccgcgcac tggtggtcta tcaatttatg
      661 tttggaaaga aacaggccaa tccgtgtccg atgtgtactc tcgttattga cagtttgaat
      721 ggtgtcgctc atcacattgc tgaaaatgtg gacctcgtga tcgtcgctgc cgcagatcca
      781 gtcgcattgc ggaagcatgc acgacgacaa ggttgggacc ggttgcgtct acttagcact
      841 gcagaaggca gtacatttaa atatgacctg ggtggagagg accgcgaagg caatcaggac
      901 tcgacactta cggttatcac tcgcgatgct gatggaacgc ttcgacattt ctacacgggc
      961 catcctcgcg tcgcgccgga aataatggaa cgaggacttg atctgctgag tcccgtttat
     1021 aacgtcttag atctgacgcc ccaaggaaga ggaagctggt atgcgtcgtt gaactattca
     1081 ccaaacgtgc tggcagcgca ccaacattaa ggtttggagt gcgccggcct ggcggcgctt
     1141 ttccaaaatg cgttggtaat ttttcgagac ttaaagcgcc gccaggccgg cgcactccaa
     1201 aaagggggaa gctaatgttt gagaatacga aagcgttttc agggttttca gtgaatgact
     1261 tgcaaaaggc aaagcagttt tatggaaaaa cccttggact caaagttaac gagcgagaag
     1321 aggggctgga acttgaagtc ggcgggggga acaaagtctt tatctatcct aaaccgaacc
     1381 atacaccagc aacgttcaca atcttaaact tcccggtgga cgacatcgat aaagctgtcg
     1441 atcaattagg taagctaggc gtgaagtttg aacattacga ttcggaacca atcaaaacgg
     1501 atgcgaaggg catctcgcgt ggacctcatc cggacattgc gtggtttaaa gatcccgcag
     1561 gaaacattct gtcagttctg aagccataag aactttagcg aaaagttacg tcccggcgag
     1621 ttgattgcct ttaaactcaa tcgtctcgcc gggcttcatc tctcggaacc taacaatttg
     1681 ttcggagcaa agattaatta agggccagag gtcagggatc gggggtcaga ggtcaggggc
     1741 cagaaaagca atggagaatt acgatttgat atttgatagt ttctattagt ttctatccga
     1801 tctgcacctc gcgcgttcag caaatagcaa ttcttaaata tcaactcata attgtctaat
     1861 aaatcatcct gacccctaac ccctgccccc tttttgagtc tgcacctcgc gctcagcaaa
     1921 tagcaattct caattatcaa ctcataatta tccaatgatt tcttcctgac ccctgacccc
     1981 aaatccctga cccctggcgc cttcccattg gacagtttca cgccgaatcg gcaataatac
     2041 gccctttcgt tgaggctggt tgccgtcttc agggtctcgg agcgtcgctc tggccaggca
     2101 taaggagcct cacaacgcag ggctggcagt taaatctcgc cattattaga aagaatcgga
     2161 aaggaatacc tttaaagtgg aactatcgct gatcttatta gtcagcctgc tggttgtttg
     2221 ttcggcagtg ctaatacgga agaaggcaat gattgccaga cgtgtaacgt caatcgcgcc
     2281 gtcatttcgt tcgttcctcg ccggcgcggg cgccatcaca ttcgggctac tcttcgctac
     2341 gcccacattt gcccaatccc atcagggtgg cgaggccagt ttggaactac ctgatctttc
     2401 acaggtgacg ttcctcaatg gcataaccgg tcataatttg ttgctcttcg ggatcgtaat
     2461 cagcgttttg ggtctggggt ttggtctggc gatttatatg caattgcaaa aaatgccagt
     2521 ccacagggcg atgaaagaaa tctccgaact gatctacgaa acctgcaaga cctatttgat
     2581 cacccagggc aagtttatcc tgattcttga ggcgttcatc gctgtggtca tcatcctcta
     2641 tttcggagtg ctctctggaa tggagatacc acgagtcatt atcattctcg ccttcagtct
     2701 ggtaggtatc gcgggaagtt acggtgtcgc ctggtttggc attcgtgtga acacgttcgc
     2761 aaactctcgc accgcctttg caagtctcga aggcaagccg ttcccgcttt atgcaattcc
     2821 tctaaaagca ggtatgagca tcggaatgat gctgatcagc gtcgagcttc tgatcatgct
     2881 ctgcatcctg ctgttcattc ctggtagtta cgctggtcca tgcttcatcg gttttgccat
     2941 tggcgagtcg ttaggcgctg ctgcccttcg tatcgctggc ggcatcttca cgaagattgc
     3001 cgacattggt tctgacctga tgaagattgt cttcaagatt aaggaagacg atgcgcgaaa
     3061 cccgggcgtg atcgctgact gtactggcga caatgcgggc gactccgtcg gaccaagcgc
     3121 agacggtttc gaaacctacg gcgtcaccgg cgttgcttta ataacattta ttctactcgg
     3181 tgtctcaaat ccgcgcgtcc aggtgcaact cctggtctgg atttttgtca tgcgcgtcat
     3241 gatgattgtc gccagcgcgc tttcgtactt tgtaaacgac gcaatcgcca aagctcgcta
     3301 caaaaatgtc gataagatga attttgaagc gcctctgacc tcgctggtct ggataacatc
     3361 aatcgtgtcc gtggttatta cgtttattgt ttccaagctg acgattccgg ccctcagcgg
     3421 caacgaaaac ctttggtgga aactagccgt catcatttct tgtggaacac tggccggagc
     3481 gattattccg gagctggtaa aagtgttcac gtccaccgaa tcaaggcatg ttcaggaagt
     3541 tgtagtctct tcgaaagagg gcggaccatc cttgaacatt ctgtcgggtt tggtggcggg
     3601 taacttctca gcttactggc tgggaataag cattgtcgga ttaatggcca tcgcttattt
     3661 cttcgcgact atgggagacg catcgggttt ctcggttggt tctttgatgg tcgatccagt
     3721 cgccgctccg gtatttggtt tcggactcgt tgctttcggc ttcctcggta tgggaccggt
     3781 aacaatcgcc gttgactcgt atggaccagt taccgataac gcccaatcag tctacgaact
     3841 gtcgctaatc gagcagattc cgaacgtcga agaagaagtg aagaaagact tcaacatcaa
     3901 cgtaaacttt ggtagagcaa aacaccttct t
//
LOCUS       DASSFP010000136         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1669975, whole genome shotgun sequence.
ACCESSION   DASSFP010000136 DASSFP010000000
VERSION     DASSFP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669975"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1554)
                     /gene="secY"
                     /locus_tag="VFH91_02915"
     CDS             complement(136..1554)
                     /gene="secY"
                     /locus_tag="VFH91_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET6861962.1"
                     /translation="MEKFLAAVRNMFNVPDLRRRIFFTLGLLAVYRLGAHVGAPGINK
                     ARLDEVWRDVAGTLLGVLDLFSGGNFRTISVFALGVTPYITASIILQLMTVVSPQLKK
                     LQEEGEMGRQKINQWTRYLTVLLAGIQTSFVAHWLQVNGVGAPTWGFLLTTVLTLTTG
                     TMFVMWLGEQITERGVGNGISLLIFAGIVIGLPRGVQQVYERVSRGDTMEMIGVFALV
                     AALIAIIAFIVFVEAARRKIPVSYAKRHVGRELAGRQQTTMPLKINMGGVIPVIFASS
                     VLSMPQSLFAALPPDPTRPDTWWAKIWTFFQTFHASDPYYELIFLSLIVFFTFFYVSI
                     VFNVEEVADNLRKHGGFMPGIRPGRATAEYLNAILTRLTTVGAIYLALVAFVPQFMLS
                     GFKVARLPLIGNTLDAFVSTTPGLSWIPTGMNYQFYFGGTSLLILVGVAMDTVAQIEA
                     QLVMRNYEGFLGSGGRLRGRRA"
     gene            complement(1582..2031)
                     /gene="rplO"
                     /locus_tag="VFH91_02920"
     CDS             complement(1582..2031)
                     /gene="rplO"
                     /locus_tag="VFH91_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET6861963.1"
                     /translation="MSLNSLRPTKGSTHKKKRVGRGPGSGLGKTAGRGEKGQKSRSGY
                     SRKIGFEGGQMPLHRRLPKRGFTNIFKKQWLEVSLAALDQHFGENEEITPEVLHLKGL
                     IKKAKHDVVVLGNGEVSKALRVSAHRFTKSAKEKIEKAGGSAIQIEG"
     gene            complement(2064..2276)
                     /gene="rpmD"
                     /locus_tag="VFH91_02925"
     CDS             complement(2064..2276)
                     /gene="rpmD"
                     /locus_tag="VFH91_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET6861964.1"
                     /translation="MPRKAKTETAENKGSIKIQYYRSFIAAPKKHKTMVRSLGLNKLN
                     QIVERPDTESMRGAVAKIPHLLRIVE"
     gene            complement(2317..2823)
                     /gene="rpsE"
                     /locus_tag="VFH91_02930"
     CDS             complement(2317..2823)
                     /gene="rpsE"
                     /locus_tag="VFH91_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET6861965.1"
                     /translation="MRQPKQRIPAQGLDLKDQVISINRVTKVVKGGKNLSFAALVVVG
                     DEGGHVGFGTGKAREVPLAIKKGIESAKKNLIRVPLINNTLPHQLIGEYGAGRVLVKP
                     ASEGTGVIAGGAVRAVMQAVGVRDVRTKVLGSTNPHNVVRATFDALLRMKDPIELARL
                     RGKQVEEM"
     gene            complement(2931..3281)
                     /gene="rplR"
                     /locus_tag="VFH91_02935"
                     /pseudo
     CDS             complement(2931..3281)
                     /gene="rplR"
                     /locus_tag="VFH91_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            complement(3341..3901)
                     /gene="rplF"
                     /locus_tag="VFH91_02940"
     CDS             complement(3341..3901)
                     /gene="rplF"
                     /locus_tag="VFH91_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET6861966.1"
                     /translation="MSRIGKKVIVLPKGVNVTVREQELEIKGPKGTLKTPIPKGINFK
                     VEGEELRAERESHDLAALHGLARALANNAIVGVTEGFSKQMDVVGVGYKADVQGKKIV
                     FSLGYSHPIEFPLPPEVEAKAERLTTKGSIQQYQTTLTLTSIDKQKLGQVAAEMHKLR
                     KPDAYKGKGVRYADVPLKLKPGKTGK"
     gene            complement(3996..4388)
                     /gene="rpsH"
                     /locus_tag="VFH91_02945"
     CDS             complement(3996..4388)
                     /gene="rpsH"
                     /locus_tag="VFH91_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET6861967.1"
                     /translation="MTDPIADMLTRIRNAIAAKHSRVDIPASKLKLEIARIMKEEGYI
                     NNFVIKGEGLKRTIRIFLRYDARGTSSITHLQRVSTPGRRVYVGSSGIPKVLGGYGIN
                     IVSTSRGLMSGKAARRENIGGELLAQIY"
     gene            complement(4468..4653)
                     /locus_tag="VFH91_02950"
     CDS             complement(4468..4653)
                     /locus_tag="VFH91_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET6861968.1"
                     /translation="MAKKSLIAKASRRPKFSVRAYTRCKRCGRPRGYLRKFNLCRICF
                     RELALQGQIPGVVKSSW"
     gene            complement(4702..5244)
                     /gene="rplE"
                     /locus_tag="VFH91_02955"
     CDS             complement(4702..5244)
                     /gene="rplE"
                     /locus_tag="VFH91_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET6861969.1"
                     /translation="MAARLKERYQKDVAPQIAKEFSIRNPMAIPRVEKIVVNMGMGEA
                     IANAKILDTAADELRAITGQKPVVTKAKKSIASFKLRQGMPIGVVVTLRGDRMYEFLD
                     RLVSVALPRVRDFRGVSAKAFDGRGNYTIGIREQLIFPEIDFNKVDKLRGMNISIVTT
                     ARDDEQARALLKALGMPFRT"
BASE COUNT         1243 a         1469 c         1219 g         1329 t
ORIGIN      
        1 caagcgcgcc tgcgtgcctt ttccggcacc aggcgccccc attagtacaa taatcttgga
       61 catacccgac taagttttaa tttgaatcaa ggatgtgccc gattcggcag gtcaccagat
      121 tagaaatcga aaacgtcagg cccgtcgacc tcgcaatcgc cctccgctgc ctagaaaacc
      181 ttcgtaattg cgcatcacca attgagcctc gatctgggca acggtatcca tcgcgacgcc
      241 cacgagaatc agcagtgatg ttccgccaaa ataaaactga taattcattc ccgttgggat
      301 ccaggaaaga ccaggagttg tactcacaaa agcatcaagc gtgttgccga tcagaggtaa
      361 acgtgccacc ttgaaaccgc ttaacatgaa ctgaggaaca aaagccacga gtgccagata
      421 gatcgcgccg acggtcgtca acctcgtcaa aattgcgttg aggtattcag ccgtagcacg
      481 tcccggtcgg atacccggca tgaagccacc gtgcttgcgc aaattgtcgg ctacttcttc
      541 tacgttgaaa acaattgaaa cgtagaagaa ggtaaaaaag acaatcaaac tcaaaaagat
      601 caattcgtag tacggatcac ttgcgtgaaa ggtctggaag aaggtccaga tcttggccca
      661 ccaggtgtct ggtctcgtag gatccggggg caaagcagca aagagacttt gcggcatcga
      721 caaaacagac gaggcaaaga ttacaggaat gacgccaccc atattgatct tcagtggcat
      781 agtcgtctgc tgtcgtccag ccagttctcg ccctacatgc cgttttgcgt agctaacggg
      841 aatcttgcgt cgggcggctt caacaaaaac gatgaaggcg atgattgcga tgagagcggc
      901 gaccaacgcg aacacaccga tcatttccat cgtgtcgccc cggctgactc gctcatagac
      961 ttgttggaca ccacgcggta aaccgatgac gataccagcg aagatcagta gcgaaattcc
     1021 attgccgacg cctcgttctg tgatctgctc gcccaaccac atcacgaaca tcgtgccggt
     1081 tgtcagcgtc aaaacggtcg tcaaaaggaa tccccaggtg ggcgctccga caccatttac
     1141 ctgtaaccag tgcgcgacga aagaggtctg aataccagcg agcaagacgg tcagataccg
     1201 ggtccattga ttgatcttct ggcgacccat ctcgccttct tcttgaagct ttttaagctg
     1261 aggcgaaact acggtcatca actgcagaat gatggacgcg gtgatgtagg gtgtaacacc
     1321 gagtgcaaaa actgagatcg ttctgaagtt accaccagag aacaagtcca aaacaccgag
     1381 aagtgttccg gccacatcgc gccacacttc atcaaggcga gccttgttga tccccggagc
     1441 acctacgtga gctcccaggc ggtaaacagc caatagaccg agagtgaaga aaatgcgccg
     1501 tctaagatcc ggcacattaa acatattgcg tacggctgct agaaacttct ccataagatc
     1561 cttctgggaa acgctactga attatccctc aatctgaatc gcggagccac cggccttctc
     1621 gatcttttct ttagcgctct tagtgaagcg gtgcgctgac acacgcagag ccttcgacac
     1681 ctctccgtta ccgagcacga ccacatcatg tttcgctttc ttgatcagac ctttcaggtg
     1741 caacacctca ggcgtgatct cttcattttc gccgaagtgc tgatcgagcg ctgcaaggct
     1801 gacttcaagc cattgcttct tgaagatatt agtaaagcct cgctttggaa gacgacggtg
     1861 cagaggcatc tggccacctt caaagccaat cttgcgagag tagcctgaac gtgacttttg
     1921 acctttttca cctcggccgg ccgtttttcc caatcccgaa cctgggccac gaccaactct
     1981 tttcttctta tgcgttgagc ccttcgtagg tcgcagactg ttaagactaa ttggcatcat
     2041 caactccgta tgtaagcgta aaactattcc acaatcctca gcaggtgcgg gatcttagcg
     2101 acggcgccgc gcatcgactc tgtgtcggga cgctcaacga tctggttcaa cttattcaaa
     2161 cctaagctgc gaaccattgt tttatgcttc ttaggcgcag caataaagct tcgatagtac
     2221 tgaatcttaa tgctgccttt gttttctgca gtttctgttt tcgccttacg tggcatagca
     2281 aatcccttcc cgactgtggc cgctcacggc caaactttac atctcttcga cctgttttcc
     2341 tcgcaaacgg gccagctcaa tcggatcctt cattcgcaac aacgcgtcga aggtagcacg
     2401 caccacgtta tgaggattag ttgagccaag gaccttcgta cgaacgtctc tcactcctac
     2461 tgcctgcata actgcacgaa ccgcgccccc ggcaataacc ccggttcctt ctgaggccgg
     2521 cttgaccaga acgcgtccgg cgccgtactc accgatcagc tggtgcggca gggtgttatt
     2581 aatcaacggc accctgatca aatttttctt cgcggattca atgccctttt taattgctaa
     2641 aggcacttca cgagctttgc cggtaccgaa accaacatgg ccaccttcgt cgccaactac
     2701 gacaagagca gcaaacgaga ggtttttacc tccctttacg accttagtaa ctcggtttat
     2761 ggaaattacc tgatctttaa gatcgagtcc ctgcgcggga atgcgttgct tgggttgtct
     2821 catagctatt ctttactttt cttacgagcc ttcttttcag ttgcttgctc aggtgcttcg
     2881 ctggcctttg attcggcagc ttcggcttcg gcgactactt cgttcttgtt caatccggca
     2941 gcccgggcag catcagtgag cgccttgatt ctgccgtgat agagataacc accgcggtcg
     3001 aaaacaactt gttcgattcc tttggacaat gcgcgctcag ctatcgcttg accgatccgg
     3061 cgtgctgcat ccaggttacc gccagtgcta cctcgcaagt ctttttcagt ggtcgaagca
     3121 gataccagag ttgttccttc acgatcatca attacctgag cgtagatgtg attcagactc
     3181 cggtaaatcg ccagtcgtgg tctgccagaa cttcccttca cttttcttcg aatgcgagag
     3241 tgaatcgcgt ttcgcacaac tgctctattc gtgtttgcca tctttcctca cttcaacttc
     3301 caatcacttc gaaatgtaaa gcgatcggtc ttggcccaaa ctacttacca gtcttacccg
     3361 gcttcaactt aagcggaacg tcggcgtacc gcacaccttt tcctttatag gcatcaggct
     3421 ttcgcagctt gtgcatctcg gcagccacct ggcccaactt ctgcttatcg atgctggtca
     3481 aggtcaacgt ggtctgatac tgctgaatgc ttcccttcgt cgttaaacgt tctgcctttg
     3541 cctcgacctc aggcggcaag ggaaactcga taggatggga gtatccaagg gagaagacaa
     3601 tcttctttcc ctggacgtca gctttgtagc caacgcctac cacatccatc tgcttactaa
     3661 aaccttcagt aacaccgacg atggcgttat tcgcgagcgc gcgtgccagg ccatgaagcg
     3721 cggcgaggtc atgcgactct cgttcagcac gcaactcttc gccttcaacc ttgaagttta
     3781 tgccctttgg gatcggcgtt ttcagcgtac ccttcggacc ttttatttca agctcctgct
     3841 cacgaacggt gacattgaca cccttcggta aaacgatgac tttctttcct atacgcgaca
     3901 tattctagtt cctgttttcc gtctccattt tccgattgcc atctggttgc acggggcgat
     3961 cagcaatcaa tcgttcctct ttggaaactg gaccatcagt agatctgcgc taacaactct
     4021 ccgccgatat tctcgcgacg ggctgcttta ccgctcatta agcctctgga cgttgagacg
     4081 atgtttattc cgtagccacc aagaactttg ggaataccac tcgaaccgac gtatacgcgc
     4141 ctgccgggcg tcgaaactct ctgcagatgc gtgattgaag aggtgccacg ggcgtcataa
     4201 cgaaggaaga tgcggatcgt acgtttcagg ccctctccct taatgacgaa gttgttgata
     4261 tagccctctt ctttcatgat gcgggcaatc tcgagtttga gctttgaagc cgggatatca
     4321 acacgcgagt gcttcgctgc tatagcgttg cgaattcgcg tcaacatatc ggcgattgga
     4381 tcagtcatga atattaactc cgttagcttg ccaattatcc ggtgggctga tgtatcagat
     4441 cgctgccaac cgtcaccgca atttctttta ccaactcgac ttaacaacgc cagggatctg
     4501 gccctgcaac gccaactcac gaaaacagat gcggcacaga ttgaactttc gcaagtaacc
     4561 tcgcggccgg ccgcagcgtt tgcagcgagt gtaggcgcgc accgaaaact tcggcctgcg
     4621 tgacgctttt gctatcaatg atttttttgc cataagtcct ttaagtctca agcatggttg
     4681 cagttcttat tgcagcaacc gttaagttct gaagggcatg ccgagggcct ttaacagcgc
     4741 tcgcgcctgt tcatcatctc ttgcggtagt aacaatcgag atgttcatac cgcgaagttt
     4801 gtcgacctta ttgaagtcaa tttctggaaa aatcaattgc tcacgaattc cgatagtgta
     4861 gttgccacga ccatcaaaag ccttcgcaga aacgccgcga aaatccctaa ctcgtggcaa
     4921 cgcgaccgag accagtcgat cgagaaactc gtacatccgg tctccgcgta aagtgaccac
     4981 gacgccgatc ggcatccctt gcctcaactt gaaggaggcg attgatttct tcgctttggt
     5041 gaccaccggt ttttgtccgg tgattgctcg caactcatct gcggcagtgt cgagaatctt
     5101 tgcgttcgcg atggcttcgc ccatccccat gttgacaact attttctcga cgcgtggaat
     5161 cgccatggga ttgcgaatac taaactcctt ggcaatctgc ggagccacgt ctttttgata
     5221 tcgctctttt aaccttgcag ccatttcaaa tcctcgccag
//
LOCUS       DASSFP010000137         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1075809, whole genome shotgun sequence.
ACCESSION   DASSFP010000137 DASSFP010000000
VERSION     DASSFP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075809"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFH91_02960"
     CDS             complement(<1..1081)
                     /locus_tag="VFH91_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6861970.1"
                     /translation="MVATEMKTNMDLMETVLLNPGQKGLRAAEFEQKLAQRIVGQDPA
                     VRCMSDLYQVFLAGMNSTNRPVGTLLFLGPTGAGKTRVVEAAAEVLFGNPNAMIKIDC
                     AEFQHSHEISKLIGSPPGYLGHRETHALLTQTNLDRYHTDETKLSFVLFDEIEKASDS
                     VWQLLLGILDKATLTLGDNSRTDFSRTIVIMTSNLGAREMSELTSAAIGFACAKDRKQ
                     SSDPGMDQKIYRTAVEAARHNFTPEFMNRIDRMIVFRSLTEQNLRRILDLELQAVQDR
                     IMTSARTKFLFGCSDSAKDRMLNEGIDLRYGARHLKRAIERLLVYPLSNLVATEQVAF
                     GEMISVDVDHQNGELIFLKSPEEPLI"
     gene            1546..1911
                     /locus_tag="VFH91_02965"
     CDS             1546..1911
                     /locus_tag="VFH91_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6861971.1"
                     /translation="MSERANVAIVDDDQSIREAIKSLLDSYGLYAEEFSSAEAFLDSG
                     CLKAFDCLILDVRMPGLGGLELQRRLAAMNSRIPIVFITAHYSDGERTKAIEAGAVAF
                     LNKPFTEEALLSAIGFSSE"
     gene            1982..2974
                     /locus_tag="VFH91_02970"
     CDS             1982..2974
                     /locus_tag="VFH91_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6861972.1"
                     /translation="MLQTVKTSVYTGWPSSGFNQEAWRKAVRRRPEAVVRPASVSDIA
                     LLYPEAVVASSDALAWQDIRMVQLRHGLSEIVVPPSDNHCLVLNLSAPLDISAEFAKR
                     NYRGKVRAGKVAIIPAGTSWSCQTHGSLVRNMLIMYLRPLFVRKIAEEFDGPFKDLAL
                     TPRFGVQGKHIRHIGMSLLGELNEANAVGRLYADSLAVSLTLQLIQHYSSSKDVRIDR
                     GGMAPHKLRKAVGWIDHHVAGEEEGRVALRAVAKDVGLSYFHFSRAFKQSMGMTPTNY
                     IAERRIERAKELMKETDLAISEIALRLGFSSQSHFTTSFRRLAGLTPRSFRKAM"
     gene            2984..>3403
                     /locus_tag="VFH91_02975"
     CDS             2984..>3403
                     /locus_tag="VFH91_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6861973.1"
                     /translation="MSIRSSLAELLADPGSSGPAILSTSPLIEVSYQALADQVDRLSG
                     QLISAGLKPGNCVAIVLPNSLEFLVIFLALTRARLVALPLNPAYKREELRFFMEESEA
                     QAVVTQGDNAVLRKTIAGIGLPVWPTRVDSRGGVELLE"
BASE COUNT          824 a          848 c          903 g          828 t
ORIGIN      
        1 tgatcagtgg ttcttcagga ctctttagaa aaataagctc accattttgg tggtcaacat
       61 caaccgagat catttccccg aacgcaactt gctctgttgc tacgagattc gagagtggat
      121 aaacaaggag tcgttcgata gcccgtttca agtgtcgcgc gccatatcgg aggtcgatcc
      181 cttcattcaa catcctgtcc ttagcgctat cggagcagcc aaaaagaaac ttggttcgag
      241 ctgacgtcat aattcgatcc tgtactgctt gtagttcgag atcgagaatc cggcggaggt
      301 tttgctccgt caaggaccga aacacaatca tcctgtctat ccggttcatg aactctggag
      361 tgaagttatg tctggcagcc tctaccgccg ttcggtagat cttctggtcc ataccgggat
      421 cgctcgattg cttccggtct ttcgcgcaag cgaagccaat cgcagccgac gtcagttccg
      481 acatctcacg ggctccaagg tttgaagtca tgatgacgat agtgcgcgaa aagtccgtac
      541 gtgaattatc ccctaacgtc agggtagcct tgtcgagaat accaagcagc agttgccaca
      601 cgctgtcaga ggctttctca atctcgtcaa acaagacaaa cgaaagtttg gtttcgtccg
      661 tgtggtaacg atcaagattt gtttgcgtca gcagagcatg cgtttcacgg tgaccaagat
      721 agcccggagg cgaaccaatc aacttcgaga tctcatgaga atgttgaaac tcagcgcagt
      781 cgattttgat cattgcatta ggattgccaa ataacacttc tgcagcggct tcgacgaccc
      841 gggttttgcc tgcaccggtg gggccaagga acagcagcgt cccaaccgga cgattggttg
      901 aattcatccc ggcaaggaac acctggtaaa gatcgctcat gcaacgtact gccgggtcct
      961 gacccacgat gcgctgcgct agtttctgct caaactcagc agctcgcaga cccttttgcc
     1021 ctggattgag gaggactgtc tccatcaaat ccatgtttgt tttcatttca gtggcaacca
     1081 tgactgtcct ccgctttcat catctagttt tcggtcgtgc ggaagattat tgggtttgct
     1141 gtcgcatatc tattggaacg tgaggaagtg agataggaag tttaggtcag cacattggga
     1201 gttgacgtaa gaatattgac ctaactgcac aatctccgta tgtaattcac caggatcctg
     1261 attagagccg gagaacgctg gcagactctt ttggtgggga ttggctggca tggcagaaaa
     1321 agtaccctga agtctgcctg tttggaatcc cctaagcgtt tactccaata cggtttctct
     1381 aaatcccaat acaattacct gacgttccaa tagttatccg attacataac taatattctt
     1441 caaccgtcgc aatgacggta aggaatgagc cggggctgaa gcgacaacgc agaactgtgg
     1501 ggggacagtc agcctcggtt aaggaatatg cgagggaaca aattggtgag tgagagagca
     1561 aatgtggcaa tcgttgatga tgatcaatct atccgagaag caatcaagtc gcttctcgat
     1621 tcttacggac tctatgctga ggagttctca tcagccgagg ccttcttgga ttcgggctgc
     1681 ctcaaagcat ttgactgttt gatactggac gtcaggatgc ctggattggg cggactcgaa
     1741 ctgcaacgcc ggctggccgc aatgaacagt cgaatcccaa ttgtgttcat aaccgcgcac
     1801 tacagtgacg gagagcgaac taaggccatc gaagccggcg cggtcgcttt cctgaataag
     1861 ccattcacag aggaagcatt gctcagcgcc atcggtttct cgtcagagtg aaatgaggat
     1921 gttaagtaat tgcttcccca tgatccgatt aaatttagtc tgaagaatag agaggaacgt
     1981 gatgctacaa acagtcaaaa cttcggttta cacaggttgg cccagctcag gcttcaatca
     2041 agaggcgtgg cgaaaggcgg tccgccgaag gccagaagcg gttgtacgtc ccgcttcggt
     2101 cagtgatatc gcactgctct acccggaggc agtcgttgcg tccagtgatg ccctcgcttg
     2161 gcaggacatc cgcatggtgc aattacggca cggtttgagc gagatcgttg ttcccccgtc
     2221 ggacaatcac tgtctcgttt tgaatctgag cgcacctctc gacatcagcg ccgagtttgc
     2281 gaaacgtaat taccgaggca aggtgcgtgc aggtaaagtt gcgattattc cagcaggtac
     2341 gtcatggtcg tgccaaacac acggatcgct cgtacgcaac atgttgatta tgtatctccg
     2401 cccacttttt gtgcgaaaga tcgccgagga attcgatggt ccattcaagg accttgcgct
     2461 tactccgcgt tttggggttc aaggcaaaca cattcgacat atagggatgt ctctgttggg
     2521 cgagttgaac gaagcgaacg ctgtgggtcg actctacgcc gattccctgg cggtgagtct
     2581 caccctgcag ttgatccaac actacagttc atcaaaagat gtcaggatcg accgcggtgg
     2641 tatggcgccg cacaagctaa gaaaagctgt cggttggata gaccatcacg tagccggcga
     2701 agaggaggga agggtggccc ttcgtgccgt agcaaaagac gtaggtctga gttatttcca
     2761 cttttctcga gcctttaagc agtcgatggg aatgactccg actaactaca tcgcggagcg
     2821 acgtattgag cgcgcaaaag aactaatgaa agaaacagac ctggcgatct cggagattgc
     2881 tttgcgattg ggattctcga gccagagtca ctttacaact tcctttcgtc gccttgcggg
     2941 attgacaccg agatctttca gaaaggcgat gtaggaacgc cagatgtcaa taagatccag
     3001 ccttgcagag cttctcgcag atcccggatc ttccggcccg gcgattctct cgacctctcc
     3061 tttaatagag gtcagctatc aagcgctggc ggatcaggtt gacaggttgt ccggccaact
     3121 gatcagtgcc ggactaaaac ctggcaactg cgtcgcgatt gttttgccaa acagcctgga
     3181 gtttcttgtc atctttctcg cactgactcg cgcaaggttg gtcgcgttac cgctcaaccc
     3241 agcctacaag cgcgaagaac tgcgcttttt catggaagag tcggaggcgc aagcagtcgt
     3301 cacccagggc gacaacgctg tgctgaggaa gaccatagct ggcattggtt tgccggtgtg
     3361 gccaacgcga gtcgactcgc gcggcggcgt ggagttgctg gaa
//
LOCUS       DASSFP010000138         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1671808, whole genome shotgun sequence.
ACCESSION   DASSFP010000138 DASSFP010000000
VERSION     DASSFP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671808"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VFH91_02980"
     CDS             complement(<1..1312)
                     /locus_tag="VFH91_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6861974.1"
                     /translation="MEKSHNSASSNRTLLMFIAFVGIFLYGLLAALPGSVLPTLERNR
                     FLPNDSAIGTFLLINAVGAVLAYLISGPLIDRIGKKFALSFGTLLVIVSMFGFGLIVT
                     SVHATGALVYIFIASFILGLGANAIVAAGHALVADVAANWRNSALNLLDICFGLGLAS
                     LPLVVQTLQRRGGLGLIFWSLGLLSLLVFLLILTSRMPQSAHSESFEIGNAAQLFKNP
                     SFLLLAFALFMYVGAEVSVGKWVVTFMQRDPAILASQGADAATLRLLSSTATESVSKF
                     FEADSVGYAIATYSYTTLTLFAMALLIGRLVSSILLGVFRFNSFVLMTLGSLLTAASL
                     LVALNASSAVTVRAGLIAAGFGMGPIFPTSVGLASVMMPKLAGTAMSLVMGVGFAGLL
                     VIPPAVGYVSSAVGGPTGNVRSGLTVVIAASIIMFVLHIVLMLRE"
     gene            complement(1367..2611)
                     /locus_tag="VFH91_02985"
     CDS             complement(1367..2611)
                     /locus_tag="VFH91_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6861975.1"
                     /translation="MAANRLGVGFIGSGFVAKFHLQAWRGVRDADVLGIYSPNRSHAE
                     ETAALARNLRVGNARAFDSIEEMVAAPEIDCIWLCGPNHQRVENMQAICDAIASGKGK
                     LTGIACEKPLARNAGEARKMVELVEKSGLLHGYLEDQMFAPSVTRGKEIMWTRAAAIT
                     GRPYLARAAEEHSGPHSPWFWRGELQGGGVLNDMMCHSIEVARFMLTAPGAPRSSIRP
                     VKVSAQIASLKWSRPEYVANLKKNMGPEVDYEQTPTEDFARATINYIDKDHNPLIVEV
                     TTSWSYVGPGLRLSLEVLGPEYSLAMNSLDSGLKLFLSREVKGNVGEDLVEKQNAEQG
                     LMPVLGNEAAEYGYEAQNRYFVQCFIDGKQPEESFHDGLEITELLMTAYMSAEQDQTI
                     NFKPGGLDSYVPLVAQGRWKGR"
     gene            2814..>3489
                     /locus_tag="VFH91_02990"
     CDS             2814..>3489
                     /locus_tag="VFH91_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861976.1"
                     /translation="MSSVRAQEKKLGVFQGRNDVGSPARAGNTVYDAEKQEYVVEGAG
                     SNMWANRDEFQFVWKQLKGDFILTSTAAFIGKGVEQHRKIGWIVRSSMDPDSAQASVV
                     LHGDGLTSLQFRRTKNGTTEELRSKVQGPDVLQLERKGNTYKMSVARSGDALSSEEVS
                     LDLGDDVYVGLFVCSHNKNVAEKAVFKNVRITVPAPDNFTPYRDYIGSNLEVMDVTTG
                     IRKVVYS"
BASE COUNT          936 a          918 c          840 g          795 t
ORIGIN      
        1 gttcccgcaa catcaataca atgtgcaaca cgaacattat gattgatgcg gcgataacaa
       61 cggtcagacc cgaccgaaca ttgccggttg gaccaccaac agcgctcgaa acataaccga
      121 cagccggcgg tattacgagc aaacctgcaa agcccacgcc cattacgagg ctcattgcgg
      181 tacccgcaag tttaggcatc atgacagagg ccaaacccac tgaggttgga aatattgggc
      241 ccataccgaa accagctgca atgaggccag ccctaacggt taccgctgaa ctcgcattga
      301 gcgcaaccag tagtgacgct gcggtcagca atgagccaag agtcatcaag acaaaactat
      361 tgaagcgaaa aaccccaaga agaatactgg aaacaagacg accaataagc agcgccattg
      421 caaacagggt tagggttgtg tacgagtagg ttgcaatcgc gtatccaacg gagtcagcct
      481 cgaaaaattt gctgaccgac tcagtcgcag ttgaactcaa taatcgtagc gttgccgcat
      541 cagccccctg gctcgcaaga atagccgggt cgcgctgcat gaatgtcacg acccatttgc
      601 ctaccgacac ctcggccccc acatacatga agagcgcgaa tgcgagcagc agaaatgaag
      661 ggttcttaaa cagctgcgcc gcattaccga tttcgaatga ttccgaatgc gccgactgtg
      721 gcattcgtga tgtgaggatt aagaggaata caagcagaga caacaagcca agtgaccaga
      781 agatcaatcc caacccgccg cgtctttgca gtgtctggac caccagaggt agcgaagcaa
      841 gcccgagtcc aaagcagata tcgagtagat tcaaggccga attgcgccaa tttgccgcca
      901 cgtccgccac gagtgcgtga ccggccgcca caatggcgtt agctccgaga ccgagtatga
      961 aggatgctat gaagatatag accagagcgc ccgtcgcgtg aacgctagtg acgatcaatc
     1021 caaagccaaa catcgacacg atcaccaaca gtgtgccaaa ggatagagcg aatttctttc
     1081 cgattcgatc tattagtgga ccactgatca ggtaagcgag cacggctcct actgcattaa
     1141 tcagcaagaa tgttccgatg gcgctatcgt tcgggaggaa cctgtttcgt tcgagcgtcg
     1201 gtaataccga tcccggaagc gccgccaaca atccataaag gaagattcca acaaaggcaa
     1261 taaacatcag gagagtgcga ttgctggagg cagagttgtg tgatttttcc aattaacgat
     1321 cctcgctttg ggcaggagaa ttaagaacga aatcagctcg acgttgtcag cgtcctttcc
     1381 aacgaccttg cgcaaccaat ggcacatagc tgtccaggcc acctggtttg aaattaatag
     1441 tttggtcctg ttcagcactc atgtacgcag tcatcaacag ctctgtgatt tcgaggccat
     1501 catggaaact ctcttcgggt tgtttcccgt caataaaaca ttgaacgaag taacgatttt
     1561 gcgcttcgta tccgtattcg gccgcttcat ttccgagtac cggcatcagc ccttgttcag
     1621 cgttctgttt ttcaaccaga tcctcgccaa catttccttt gacttcacga ctgagaaaga
     1681 gttttagacc actatcaagc gaattcattg ctaacgaata ttccggccca agtacttcca
     1741 aactcagacg caaacctggt ccaacataac tccatgacgt agttacttcc acgatcagcg
     1801 gattgtggtc tttgtctatg taattaattg tcgcacgcgc aaagtcctcg gttggtgttt
     1861 gttcgtaatc aacttccgga cccatgttct ttttcaaatt agcgacatat tcaggccgtg
     1921 accacttaag actcgcaatc tgagccgaca ctttaaccgg ccggatactc gaacgcggcg
     1981 cacctggtgc ggtcagcatg aagcgagcaa cctcaatact gtgacacatc atgtcgttga
     2041 gaactccgcc gccctgcagt tcaccgcgcc agaaccacgg cgaatgtggt ccactgtgtt
     2101 cttccgccgc gcgtgcgaga tagggccgac cagttattgc ggctgccctg gtccacataa
     2161 tctctttgcc gcgcgtaacg ctcggagcaa acatttgatc ttcaagatat ccatgcagta
     2221 atcctgactt ctcgaccaac tccaccatct tgcgagcctc tcccgcattt cgagccagag
     2281 gcttttcaca ggcaattccg gtcagtttcc ctttgcctga tgcaattgcg tcacaaatgg
     2341 cctgcatgtt ttcaacgcgt tgatggtttg gaccacaaag ccagatgcaa tcaatctccg
     2401 gcgccgcaac catctcctcg atcgagtcga aagcacgcgc atttccaacc cgaagatttc
     2461 gagcgagcgc ggctgtctcc tcggcatgag accggttcgg gctgtagatg cccagtacat
     2521 cggcgtctcg cacaccgcgc caggcttgta agtgaaactt tgcgacgaaa ccactaccga
     2581 taaagccgac tcccaatctg ttagctgcca tgtgtcgaat tctccggtta aataattgct
     2641 cgttaaaaac agaccttgtt ggttacaatc cgccgtaccg tcaatttaca gaccagctta
     2701 ttcgcttcag ctagctgcgt ctaacttaat tcaacattaa gaatccggtg agccaaatgc
     2761 tattacaaat gagaactgta aaagctcttc ctgttttaat tcttcttata acattgagca
     2821 gcgtacgagc gcaggaaaaa aaactcggag tgttccaggg caggaatgac gtcggcagtc
     2881 cggcccgtgc cggtaatacc gtgtacgacg ctgaaaaaca ggagtacgtg gttgaaggcg
     2941 caggctcaaa tatgtgggca aatcgcgacg agtttcagtt tgtttggaaa caacttaagg
     3001 gagattttat tctcaccagc actgccgctt ttatcggcaa aggtgtcgag cagcacagaa
     3061 aaattgggtg gatcgttagg tcgagcatgg atccagattc tgcccaggca agtgtggtct
     3121 tgcacggcga tggtttgacc tcacttcaat ttcgcaggac caagaatggg accaccgagg
     3181 agttgcggtc taaagttcaa ggtccggacg tactgcaact cgagaggaaa ggaaatacct
     3241 acaaaatgtc agtggcaagg tctggcgatg cgctaagttc tgaagaagta tcgctcgacc
     3301 ttggcgacga tgtttacgtt ggactctttg tgtgctccca caacaaaaac gttgctgaaa
     3361 aagctgtgtt caaaaacgta cggattaccg tgccggctcc ggataatttc acgccttatc
     3421 gtgactatat cggaagcaat cttgaagtaa tggacgttac cactgggatc agaaaggtgg
     3481 tctattccg
//
LOCUS       DASSFP010000139         4157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_626912, whole genome shotgun sequence.
ACCESSION   DASSFP010000139 DASSFP010000000
VERSION     DASSFP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626912"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFH91_02995"
     CDS             complement(<1..612)
                     /locus_tag="VFH91_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6861977.1"
                     /translation="MHTDSQLLAQLLDAGVDGVFAFDREMRVIAWNKAMTRITGISRE
                     EIVGKNSLVTSRVIGDLVSEPHLQKALAGESVVSEKSVPSLSPAIYRLHYSPLRNNEG
                     SVTGGIAVLRNITEPTQTQDDAHDAYRRLKFHVESSPLAVIEWDSDFRVSRWSESAVR
                     LFGWTAEEVIGKHVNEWQFVFADDVDAVELVTNRQRQGFEVHGL"
     gene            complement(835..2094)
                     /locus_tag="VFH91_03000"
     CDS             complement(835..2094)
                     /locus_tag="VFH91_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6861978.1"
                     /translation="MTVKTSKEFLIVLSGFLLTLATTSIAFGQDWTQWRGPKRDGSVT
                     GAGSPKIWPDALHRVWRVEVGEGYSSPVVSAGRAFIHGRRDPEEVVLAIDLADGKILW
                     EKKYQANFKKNQYAVEMAKGPNATPLILGNRLFTLGVTGIVTAWDTSNGQQLWSRDFS
                     KTIDTSKLFCGTAASPLSVNGKVVIQVGSDLHGGEIMALDPATGNTSWEWRGLGPGYA
                     SPIVVPIDNKEQIVSMTEGSIVGVDPGTGKELWSVLFPDEWHENISTPLWTGSQLIVS
                     GPRQGTHAYSISNTAGKWQVKETWKNPDVAMYMSSPVFGDELIYAHSSKRKGQFVALD
                     VKTGTVRWATEGREGEHASLLLTPQNVVFLTNGADLIVAKRGGTSFSVEKRYEVADGS
                     TFAMPVLLGPDVLVRDATGLIRLAPGK"
     gene            2322..2900
                     /locus_tag="VFH91_03005"
     CDS             2322..2900
                     /locus_tag="VFH91_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET6861979.1"
                     /translation="MLTLIVAFLLTFFTAANIYSYGNTSSEVKADAAIVLGAAVWGNQ
                     VSPVFRERINHAINLYHQGRIRKLIFTGGQGNRNEPTEAAAARNYALQSGVPMNDVLI
                     EQQSHTTYENMVYAKQLADQYGLKKILIVSDPLHMKRAITMARDVGLDAYPSPTPTTK
                     YRSLRIQTSELARETFYYLGYCTENLIGRPLR"
     gene            2897..3838
                     /locus_tag="VFH91_03010"
     CDS             2897..3838
                     /locus_tag="VFH91_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861980.1"
                     /translation="MKTLSTSLVLLLLCTLIMFTTECARPKIDAKATPAALHVSPTEP
                     DPSPSSKLEQHLTDLKKRLPSNDFSIVVEPPFVVIGDEPNSVVKERSEETVRWAVDKL
                     KKDYFSKDPDEILDVWLFKDAVSYEKHAQLLWGEKPGTPYGYYSRAHKALIMNISTGG
                     GTLVHELVHPFIEANFPACPSWLNEGLGSLYEKCGEEDGHIHGYVNWRLSGLQRSIKA
                     GGLASFKELMSMDAQTFYADKRGDNYAQSRYLCYYLQQKGLLFRFYKRFHANQQQDPT
                     GYKTLQTVLQENDLTRFQKRWEQYVLGLSEGFEVRVE"
     gene            complement(3851..>4157)
                     /locus_tag="VFH91_03015"
     CDS             complement(3851..>4157)
                     /locus_tag="VFH91_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861981.1"
                     /translation="LLLLATAVFSYSCARTKGAPSVAAAFTKDALVTHQTKVSFETDI
                     QPILKAKCQPCHFPGGRMYDKLPFDRAETIQKLGTKLFTRIRDEHDQKLIKQFLSQS"
BASE COUNT         1103 a         1039 c         1011 g         1004 t
ORIGIN      
        1 caggccgtgc acttcaaatc cctgacgttg gcgattggtg acaagctcga cagcatcaac
       61 gtcgtcggcg aagacaaact gccactcgtt cacgtgttta ccgataactt cttcagcagt
      121 ccagccaaac agtcgaacgg ccgactcgga ccagcgcgac actcgaaaat cgctgtccca
      181 ttcaattacc gccaggggtg agctttcgac gtgaaatttc aggcggcgat aagcatcatg
      241 agcatcgtcc tgcgtctgag tgggctcggt gatattacga agaacggcaa ttccaccagt
      301 aacagaccct tcattatttc ttaagggcga gtaatgcaaa cggtaaattg cgggcgacaa
      361 cgaggggaca gacttttccg acacaacact ttcgcctgct aacgcttttt gaagatgtgg
      421 ctccgagact aagtctccaa ttactcgaga ggtcacaaga gaatttttgc cgactatttc
      481 ctctcgagaa atcccggtga tgcgcgtcat tgctttgttc caggcgatca cacgcatctc
      541 gcgatcgaac gcgaagacgc cgtcgactcc agcgtcgaga agttgagcca gtaattggga
      601 atccgtatgc aagcgtttaa gtggggcgcg cgcctaagat tgtatcggaa aagaggagct
      661 tagcggattg cgaaatgaag aaatctggca aacatatgtt gcggtgccgg cctcgaccaa
      721 aacgtctata agtgtgaaag cgaacgatag gatagttcga aattgaccaa tcggcagcgt
      781 ttttagcccg taagggactc aacaagactg gggaagacat tgccaggaac agctctactt
      841 acctggtgca agtcgtatca gtccggtggc atcccgaacc agaacgtcgg gccccaacaa
      901 aacgggcatc gcgaaagttg agccatcagc cacctcataa cgcttttcca ccgaaaacga
      961 tgttccgcca cgttttgcga caatcaaatc tgctccgtta gtcaggaaca ctacattttg
     1021 aggagtcaat aacaaagaag catgttcacc ctctcgacct tcagtggccc acctgaccgt
     1081 tccggttttc acatccaacg caacaaactg tcccttgcgc tttgacgaat gcgcataaat
     1141 cagttcgtca ccgaacacgg gagaactcat gtacattgcc acgtctggat tcttccacgt
     1201 ctctttgacc tgccatttgc cggcggtatt tgaaattgag tatgcgtgag ttccttgccg
     1261 agggcccgaa acaatgagtt gagagccagt ccacaaagga gttgaaatgt tctcgtgcca
     1321 ttcatccgga aagagcacgg accacaattc cttacctgtt cccggatcga ctccgacgat
     1381 ggaaccttct gtcattgaaa caatctgttc tttgttatcg attgggacca caatcggaga
     1441 agcgtagcct ggtccaagac ctcgccactc ccatgacgta ttacctgtcg ccggatctag
     1501 agccatgatc tctccgccgt gtaaatcgct cccgacctga atcacgactt ttccgttgac
     1561 tgagagtgga gaggcagcag tgccgcagaa taatttcgag gtgtcgatcg tctttgagaa
     1621 atcccgggac cacaactgct gaccattgga cgtatcccat gctgtaacaa tccctgtcac
     1681 tcccagggta aacaatcggt tgccaaggat tagtggagtg gcatttggac ctttagccat
     1741 ttcgacggcg tactgattct ttttgaagtt tgcctgatac ttcttttccc acagtatctt
     1801 tccatcggcg aggtcaatcg caaggacaac ttcctccggg tcacgtctac cgtgtatgaa
     1861 agcccgacca gccgacacaa caggtgacga gtaaccttcg ccaacttcca ctcgccacac
     1921 acggtgaagt gcatcaggcc agatctttgg actgccagct cctgttacag atccatcgcg
     1981 ctttggcccg cgccattgag tccagtcttg gccgaatgct atcgaagtcg ttgccaatgt
     2041 caggagaaat ccggacaata cgattagaaa ctctttcgat gtctttacag tcatagttga
     2101 tctctttctc gcgacgcttg gatggctaga ataagaagca gcagaaacca aaagctatcc
     2161 ggggatgcgg gagattctgg cagagctcag gcaaaccaac aagtaaaaag tacacgtaca
     2221 ataaactgaa tttgccgtga cttattttgt acgcactttc atccgtttag acgttaatcg
     2281 acatccaaat caacatgcgc cgacggctcc ttaaaatcct ggtgctaact ctcatagtag
     2341 cgttcctctt gacgtttttt acggctgcga acatctattc ctacggcaat acatcgagtg
     2401 aggtaaaggc tgatgcagca attgtactgg gcgcagcagt ttggggtaat caggtgtcac
     2461 cggtattccg ggaaagaatt aatcatgcca tcaatctcta ccaccaggga cgaatccgaa
     2521 aactgatctt tactggcgga caaggtaatc gcaacgaacc aacggaggct gcggcggcac
     2581 ggaattatgc actacaaagc ggagttccga tgaatgatgt tctgattgaa cagcagtcgc
     2641 acactaccta tgaaaatatg gtctatgcca aacaactggc agaccaatat ggtctaaaga
     2701 aaatcctaat cgtaagcgat ccattgcata tgaagagagc cataacgatg gctcgcgacg
     2761 ttggtttgga tgcataccct tcaccaactc ccacgacgaa atatagaagt ttaaggattc
     2821 aaacttcaga acttgctcgc gagactttct actatctcgg atactgcacc gagaatctga
     2881 tcggtcgtcc gttgcgatga agacgctatc aacatcttta gtgctgttgt tgctttgtac
     2941 tctcatcatg ttcacgactg agtgtgcgcg gccaaagatt gatgcgaagg ctactccggc
     3001 cgccctacac gtatcgccga cggaacccga tccttctccc tcgtcgaaac tcgaacaaca
     3061 tctaaccgat ctaaaaaagc ggttaccgtc gaacgacttc tcaatcgtag tcgagcctcc
     3121 gtttgtggta atcggagacg agcccaactc ggtggtgaag gaacgatcag aagagacggt
     3181 tcgttgggca gtagataagt tgaagaagga ttatttcagc aaagaccccg atgagatact
     3241 cgacgtctgg ttgttcaaag atgccgtttc gtatgaaaaa cacgctcaac tgttgtgggg
     3301 agaaaagccg ggaacacctt atggctatta ttcgagagct cataaagcgt tgatcatgaa
     3361 catctccacg ggcggtggga cgcttgtgca cgagttggtc catccattta ttgaggcaaa
     3421 ctttccagcc tgcccatcat ggttgaatga aggtctcggc tcgttgtacg aaaagtgcgg
     3481 cgaggaagac ggacacattc acggttacgt caattggcga ttatcgggat tgcaacgatc
     3541 gatcaaagcc ggaggattgg cttcattcaa agaattgatg tcgatggatg cgcagacttt
     3601 ctatgccgat aaaaggggag acaattacgc ccagtcgcgt tacctctgct actaccttca
     3661 gcaaaaagga cttctcttta ggttctataa gcgatttcac gccaaccaac agcaggatcc
     3721 aaccggatat aaaacactac aaactgtgct tcaggagaac gaccttactc gctttcagaa
     3781 aagatgggag caatacgtgc ttggcttaag cgaaggtttc gaggtgcgag tcgagtaggt
     3841 agttgcgcta ttacgactgg gaaagaaact gcttgatcag cttctgatcg tgttcgtcgc
     3901 gaattcgtgt gaagagtttt gtgccaagtt tttggattgt ctctgcacga tcaaatggaa
     3961 gtttgtcgta catccgacca cctggaaagt gacacggctg gcatttggct ttaagaatcg
     4021 gctgaatgtc agtctcgaaa ctgacctttg tttgatgagt gaccaatgca tctttcgtaa
     4081 aagcggctgc tacagatggt gctcccttcg tacgagcgca cgagtacgag aaaactgctg
     4141 tggccagtag caatagc
//
LOCUS       DASSFP010000140         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_481337, whole genome shotgun sequence.
ACCESSION   DASSFP010000140 DASSFP010000000
VERSION     DASSFP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481337"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1084)
                     /locus_tag="VFH91_03020"
     CDS             complement(278..1084)
                     /locus_tag="VFH91_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6861982.1"
                     /translation="MFERVEESPILEPESSNADTAVCDQCFGTGTKLDPVKGAILCPC
                     KTQGRSQKLLRAARIPARYANCSLDNFRVTRDSSQERAFLYAGRLVLDFPAVDKGLLF
                     MGPAGVGKTHLAVAIIKGLLAKGFPCLFCEFGSLLKEIQESYNPISKSSELKVLAPVY
                     QAEVLVLDELGATMPTDWVRDTMYQIINKRYNDRKLTIFTTNYQDEIRTDLKSQSDAG
                     DRPIGRREASERIREMARLEDRIGTRLRSRLYEMCTEVKIEGDDYRRKKV"
     gene            1255..1701
                     /locus_tag="VFH91_03025"
     CDS             1255..1701
                     /locus_tag="VFH91_03025"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HET6861983.1"
                     /translation="MNSLLAKLGLASIKAERHPIRVFLLDDDQRRHQWFTTRFKGDLL
                     DIADNVVQAQEMLSAKSYDAIFLDHDLHPEHYHSDSTDDERTGYAIAMWLSANPDKQP
                     ASTILVHTRNADGAMRMVEELRRAGRSAEYVPFPILADRIKRYWQR"
     gene            complement(1914..2183)
                     /locus_tag="VFH91_03030"
     CDS             complement(1914..2183)
                     /locus_tag="VFH91_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6861984.1"
                     /translation="MSMKLYVGNLSFETSSSELQTLFAQTGTVESASVIEDRETGRSR
                     GFGFIEMSTKEEGVAAIEKFNGHEVGGRALKVNEAKQRENRPAYN"
     gene            complement(2288..>2467)
                     /gene="groEL"
                     /locus_tag="VFH91_03035"
     CDS             complement(2288..>2467)
                     /gene="groEL"
                     /locus_tag="VFH91_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6861985.1"
                     /translation="VTEKFEDLVAAGVIDPAKVTRCALQNAASIAGLMLTTEALISEL
                     QDDDKPRAMPGGMDM"
BASE COUNT          598 a          654 c          569 g          646 t
ORIGIN      
        1 cccccagctg gagttgcagg ctcgttaaga tcccgcctcg acttccagta gattaagttc
       61 gatttttatg gtttgtcatt gcgagtaggt tggactttaa ggacccccaa ctcccgttgg
      121 gggaattttg ctagttggcc gacggttgtt tggtaggcag gacttgaact atctcccgac
      181 taccttgggg gaattcgctg attacagttc aagactcggt taaaggtaag taagccaacg
      241 ggagttttgg gaggttaatg cgcgggagcg ttacaagcta aactttttta cgccggtagt
      301 cgtcaccttc aatttttact tcggtacaca tttcgtaaag acggctacgc aatctggtcc
      361 caatgcgatc ctcaagtctg gccatttcac ggatccgctc cgaagcttcc ctacgaccta
      421 ttggccgatc accagcatcc gactgactct tcaggtcagt tcgaatttca tcctgataat
      481 tagtcgtgaa gattgtcagt ttcctatcgt tgtacctttt attaatgatc tggtacatcg
      541 tgtcgcgcac ccagtcagtc ggcatcgtgg cgccaagctc atccagcacc aacacttctg
      601 cctgataaac cggtgcgagt actttcaatt cagacgactt cgagatcgga ttatacgact
      661 cctggatctc tttcaatagt gacccgaact cacaaaacag gcacggaaat cctttggcaa
      721 gcaagccctt gatgatggca acggcaagat gcgtttttcc cactccagcg ggtcccataa
      781 acagcaaccc tttatccact gccggaaaat caagcacaag tcgaccagca taaagaaatg
      841 ctctttcctg ggagctatca cgcgtgactc tgaaattatc caaagaacag ttcgcatagc
      901 gcgctggtat acgagccgcc cgcaataact tttgcgaacg cccctgtgtt ttgcaaggac
      961 aaagaatggc gcccttcacc ggatcaagct ttgttcccgt gccaaagcac tgatcacaaa
     1021 cagcggtgtc cgcgttgctc gactcgggtt ctaagatcgg tgattcttcc acgcgctcaa
     1081 acatatacgt ctactctaac aagctttgcg tatggattat aacacccgca atttgaaaat
     1141 tgagcaacgc ttgtttttta acgacattcc agccgcggtc ttagtcagga aaagcggtat
     1201 gttatattcg gcattctcct caattcgatt ttagattagc cacgtatgca actgttgaac
     1261 tctcttttag ccaagcttgg gctagcttcc atcaaagccg aacgccatcc aatccgtgta
     1321 tttctacttg acgatgacca gcgacgacat cagtggttta cgactcgctt caagggagac
     1381 ctccttgata tcgctgacaa tgtcgttcag gcccaggaga tgttgagtgc gaaatcgtac
     1441 gatgcgattt ttctcgatca cgatctacat cctgaacact accattccga ctcgaccgat
     1501 gatgagagaa ctggttacgc gatagcgatg tggctaagtg caaacccgga caagcagcct
     1561 gcttcgacaa ttcttgtgca tacccgaaac gctgacggag cgatgcgaat ggttgaggag
     1621 ttgcgacgcg ccggccgctc cgccgagtat gttcccttcc ccatcctcgc cgatcggata
     1681 aagcgctact ggcaacgata agcgaagagg atcatccaca gattgcgctg attacgccga
     1741 tttttaaaac gctgacgagt tagaaaccac catgtgagct caaaatagcg tgagggcaaa
     1801 cgagatgtat ggactctatc aggagtgctg tgttatagcg tgtgggccat acgaaagaaa
     1861 aagatctcgg gctaattaaa gtccgagatc cgttcagatt tttcgaggtg ctcttaattg
     1921 taagcaggac gattttcacg ttgcttggct tcgttgactt tcaaagcccg tccgcccact
     1981 tcatggccgt tgaacttctc gatagccgca acgccttctt cctttgtgga catctcgata
     2041 aagccaaagc cacgcgatcg acccgtctca cggtcctcga tcaccgacgc actttcaacc
     2101 gtaccggtct gcgcgaacag cgtttgtagt tcactactcg acgtctcaaa cgagagatta
     2161 ccaacatata gtttcataga cattttttct tacctttccc catctggggt tccctcagaa
     2221 ggacttctcc ttcgaattca acggaggcca catggtaacc atgttagcct cccaatttgc
     2281 cacggaatta catgtccatg ccgccgggca ttgcccgtgg cttgtcatcg tcctgtagct
     2341 cagaaataag ggcttcagtt gtgagcatta gccctgcgat tgatgcggcg ttttgtagtg
     2401 cgcaacgcgt caccttggcg ggatcaatta cacccgctgc cacgaggtct tcaaactttt
     2461 cggtcac
//
LOCUS       DASSFP010000141         7547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_481794, whole genome shotgun sequence.
ACCESSION   DASSFP010000141 DASSFP010000000
VERSION     DASSFP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481794"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..514)
                     /locus_tag="VFH91_03040"
     CDS             complement(296..514)
                     /locus_tag="VFH91_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861986.1"
                     /translation="MCGIIKAFGRLGGVIALILLLVALLKQLIAIVGFLLMAVKIAIV
                     VIFVAVLVLILLSILRDRSRRKRDFSEV"
     gene            complement(551..1177)
                     /locus_tag="VFH91_03045"
     CDS             complement(551..1177)
                     /locus_tag="VFH91_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861987.1"
                     /translation="MSHQSQSSASQILRRKSSPFVATLLSFVPGLGAAYNGQTSKAIV
                     HFAIFASFFQMAVITQGWQFFVLGVVGTWLFAAVDACRTAQLMRAGLSPDAEDDVIAR
                     RLYGNPIAWGTTLIIIGTLFLLHTLLRVNLPIKELLPVALVALGAYMLFDYLRRGKSS
                     DRVVRFDSRRPPPSVVGTRPVDTAAFRTGETTQLSRRDVTPLPFERRS"
     gene            complement(1334..2134)
                     /locus_tag="VFH91_03050"
     CDS             complement(1334..2134)
                     /locus_tag="VFH91_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861988.1"
                     /translation="MLCVDFESQLTDYLEAVIGPETNRLMAEHAMRCPICHETLLAVK
                     STLDACRVATVPAPSKDLEARILQKTVPETAMSCEDFEEFLTDYLDGFLPANLYHRWE
                     RHAALCDRCTELPGEVVRSIGACYTYISEEKPVPAGLNERILKATLGTDTANEIKAPW
                     SSRAASRLRLWLDPIVSPQLATVATMLLVAILVLTNTVSADGSIGGIYNASLELAERT
                     TGNAAVKQISDDLKLEQSAPPAPQQKSTDQQRDQNKSGANQHDGQSNR"
     gene            complement(2217..2819)
                     /locus_tag="VFH91_03055"
     CDS             complement(2217..2819)
                     /locus_tag="VFH91_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6861989.1"
                     /translation="MGAVKALAVSEGAELVRRCRAGDGAAWEEIVQNYSRRIYNLAYR
                     FTSRSDTAEDLTQEVFVRVYRSLDQYDPKQGDLQNWLMRLARNLIIDDYRKRQRTPHD
                     NHADDLADHSYHLHSRSNTVQQEMERRELGVQVQAGIDKLSPDLRTCVILRDIEELSY
                     QEIVDLLKIPEGTVKSRINRGRIELAKILRRMKVVAMGEA"
     gene            3103..4218
                     /locus_tag="VFH91_03060"
     CDS             3103..4218
                     /locus_tag="VFH91_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6861990.1"
                     /translation="MAKTRKYIEFGALCLLAVGILWWFGRTLNWTEVRTAVKNANVYM
                     LVGAALIISFAYVIRAFRWGAFLRPLGASHFSHLFAATTIGFGAVFLAGRAGEVVRPV
                     VLPMRDPSVRPSASFVTILVERIYDLSAVVLMFAINLVWFRPPAGLQADFNRVRFVGI
                     ALLTIAILGLIVLAWFRKNSSRVVEAFQSLFAQWTFIPQRFAKLVIALVAQLARALSV
                     LVNVTELIETVFWTALLWVGIALANLLVIRAFGVHFGFSETVFVLGWSLVASLVPTPG
                     GAAGAFHAATAAGLLFLGVEKEIAAAISILMHLIDFGPALIFGLVYVIKGDLNFSRLR
                     ALTSQEAVEDVVENEVLIPGEGHKDRQSLDNTAGEAF"
     gene            4244..4723
                     /gene="nrdR"
                     /locus_tag="VFH91_03065"
     CDS             4244..4723
                     /gene="nrdR"
                     /locus_tag="VFH91_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET6861991.1"
                     /translation="MKCPFCAYLEDKVVDSRESREGDAIRRRRECLRCERRFTSYERI
                     DEIPYMVVKKDGRRENFDRNKVMAGLLRACEKRPVASTKLDSIVNAIEKYVQESPERE
                     RPTSKIGEMIMRRLKELDKVAYVRFASVYLEFEDVSEFMTELKHLVRTRATGTAGRK"
     gene            4758..5231
                     /locus_tag="VFH91_03070"
     CDS             4758..5231
                     /locus_tag="VFH91_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6861992.1"
                     /translation="MIKPIRATGLERLELQRLRDRVGRLYAALQEATEAENPIASGAW
                     APPVDLSETPEAIVLRVELPGCSADQIMIGLCNTKLRIWGDKKRRPARRRIISYLCSE
                     RSYGKFNRVVHLRWTISVRDAEAELTNGILNIRLPKIEDRRGGEVMVPVKETSPE"
     gene            5275..>7547
                     /gene="lon"
                     /locus_tag="VFH91_03075"
     CDS             5275..>7547
                     /gene="lon"
                     /locus_tag="VFH91_03075"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6861993.1"
                     /translation="MAIPKETEFTEAIDTTASDQQLQIPSDLPVLPLRDIVIYPFMIV
                     PLFVSREKSIRAVDEALGENRMILLASQKDLDKEEPTADDLYSIGTAAVIMRMLKLPD
                     GRIRILVQGLARAQISSVDTTGEYLRARVNVIQEILPPERSLEVEALVRNVRASMEKA
                     INLGKNISPEVMAIIANLDDAGRLADLSASNLELKVEDAQSVLDIADTTARLRRVNDL
                     LNKEIEVLTVQQEINTQARADIDRSQREFYLRQQMKAIQSELGEGNELAEEIAQFREK
                     IEAAKMPKAAEDESLRQLKKLERMHPDAAETATLRNWMEIMTDLPWTRSSVDNLELQK
                     AEKVLNEDHYGLEKVKDRILEALAVRKLKEKPKGPILCLVGPPGVGKTSLGRSIARAL
                     DRKFVRLSLGGVHDEAEIRGHRRTYVGAMPGRIIQAVQQAGTNNPLIMLDEIDKVGAD
                     FRGDPSSALLEVLDPEQNNSFRDNYLGVTFDLSNVIFMTTANVLDTIQPALRDRMEVI
                     RLAGYTEEEKREIAVRHLLPKQMDENGITSKDLNISRTALIAIIQQYTQEAGLRQLER
                     EIGRICRKVARKIAEGHKGTIRVSLKNLHEFLGAPKIIPDEVLKKDQIGIATGLAWTA
                     VGGDVLFIEALRMKGKGGLVLTGQLGDVMRESAQAAYSYAKSRAKELDIAEEDFNNYD
                     LHIHIPEGAIPKDGPSAGITLATAMVSALSSRPIRRDVAMTGEITLRGNVLPIGGVKE
                     KVLAARRARVSTIILPQ"
BASE COUNT         1908 a         1835 c         1932 g         1872 t
ORIGIN      
        1 acatcgaggc agcttcagca accgaaaagc acaaactcgc caaatagcga tttgcgcaaa
       61 cctggttgat aagagacttt taactaagtt cctgcttgaa gatttgcagc ggttggattt
      121 ggaaacctca aggttctccg aggagaaatc cgtccggccg gttctgcaaa agacggctga
      181 ccctatcaca gtgccaacga acgttgcaac tttgctagtg aattataaga aagttggaac
      241 taaaaaaggc tcttcccgca agaagagccc ttttccaaaa ggttatacgg tcgtcttaga
      301 cctcgctaaa gtcccgtttt ctgcgtgatc gatcccggag tatggagagc aggattagaa
      361 ccaggacggc gacaaatatc accactatcg ctattttcac cgccattaat agaaagccaa
      421 cgatagcgat aagctgtttc agcagagcga cgagcaacag gatgagtgcg atgacgccac
      481 cgagtcttcc aaacgcttta attattccac acatagaaaa acctctgttc caagacttgc
      541 agatacaatc tcacgatctt cgctcaaacg gaagaggcgt tacgtctctg cgcgaaagtt
      601 gtgtagtttc gccagttcga aaggccgccg tatcaacggg acgggttccg acgactgacg
      661 gtggcgggcg acgagagtca aagcgaacaa cacgatctga tgatttgccg cgtcgtaagt
      721 aatcaaaaag catgtacgcg cccagagcca ctaatgcgac aggcaaaagc tctttgattg
      781 gaagattgac tcgcaggagg gtgtgcaaaa gaaagagcgt gccaatgata atcagagtcg
      841 tgccccacgc gatcggattg ccatagagcc gccttgcaat cacatcatct tcagcatcgg
      901 gcgataatcc agctcgcatc agttgagcag ttcggcaggc atccaccgcc gcaaacagcc
      961 aggttcccac cacgcccagc acaaagaact gccaaccttg cgttatcaca gccatttgaa
     1021 agaagcttgc gaagatcgcg aaatgaacta tcgctttgga tgtctgaccg ttgtaggcgg
     1081 caccgaggcc aggcacaaac gagagtagag tagcgacaaa cggcgagctt tttcgtctga
     1141 gtatttgcga tgccgatgac tgagactggt gactcaagag ctcaactcca gtcacaatgc
     1201 aatcctggca aaacggaaat cctcgcacac gatgatcaca ggcggggcac aaccatcgcg
     1261 cacactggtt gcactgcacg acggcaggat tcttcgcgtg ataactgcag tacatcatcg
     1321 tgtaacgtct tcctcagcga ttactctggc cgtcatgttg gtttgcgcca gacttattct
     1381 gatccctttg ttgatccgta gatttttgct ggggagcagg cggtgcgctt tgttccaatt
     1441 tcaggtcgtc cgaaatctgc ttaaccgcgg cgtttcccgt tgttcgttcc gcgagttcga
     1501 gactcgcgtt gtatatcccg ccaatagatc cgtcggcaga aactgtattc gtcaaaacaa
     1561 gaatcgcaac tagaagcatt gtcgcaactg ttgcaagttg tggtgaaaca atcgggtcaa
     1621 gccagagtct caatcttgaa gcagcacgag aggaccacgg cgcctttatt tcattagctg
     1681 tatcggtgcc aagcgttgct ttaagtattc gttcattcaa tcctgcagga actggcttct
     1741 cttcgctaat gtaggtgtag caggctccaa ttgatcggac cacttcgcca ggcaattccg
     1801 tacagcgatc acacagagct gcatgccgtt cccaccgatg gtagagattt gcagggagaa
     1861 atccatcgag gtaatcggtc aagaactctt cgaagtcctc gcaactcatg gctgtttcag
     1921 gcacagtctt ttgcaggata cgagcttcga gatctttcga cggtgccggc acagtagcaa
     1981 ctcggcaagc gtcaagggtg cttttaaccg ctaataatgt ctcgtgacat atcgggcatc
     2041 tcatggcatg ttctgccata agacgatttg tttctggtcc gatcacggcc tcgaggtaat
     2101 cggtcagttg agattcaaaa tcaacacaca gcatttgttg tcgctttcct gcccccactt
     2161 gatccactga ctcttgacgt tgcctgcccg cgctgaaaag aggtcagtgt ccgtttttat
     2221 gcctcaccca ttgcaactac tttcattcgt cgtaagatct tcgccaactc aattcgtccc
     2281 cgattaatcc gggatttgac cgttccttcc ggtatcttaa gaaggtcaac gatctcctgg
     2341 tagctaagct cctcaatatc tctcaagatt acgcacgtcc tgagatctgg agatagcttg
     2401 tcgatgcccg cttgtacttg cacccctaac tcacgccgtt ccatttcttg ctgcacagtg
     2461 tttgatcggc tgtgcaggtg atagctgtga tctgctaaat cgtcggcatg attatcgtgt
     2521 ggcgttcttt gacgtttacg gtagtcgtca atgatcaagt tgcgcgccaa acgcatcagc
     2581 cagttttgga gatcaccctg cttcggatcg tactgatcca gcgaacggta aactctgaca
     2641 aatacctctt gagttaagtc ttcggccgtg tcggagcgag aggtaaatcg gtacgcgagg
     2701 ttgtaaatac ggcgagagta gttctggacg atctcctccc aagccgcgcc atcgcccgca
     2761 cgacacctgc gaaccagctc agctccttcg cttactgcca aagcttttac agcccccact
     2821 ttattttctc cgggtacctg tactctgaaa atccagcaag gcggcaaacc cttgtcatga
     2881 gcggattacg acaggtccga aagtaaagtt ccgcgcaaac tatgcatagt cgcaaacata
     2941 ctcaacgtca ttccatctga caaggtattg gcaatcacca ttcttgctct tctttagagt
     3001 cctgtagtag cctttgccac cgacccaaca tgttgtggtt tggtaattga gaagtcaagc
     3061 aagtcccctc ttaagtctcg gcccagtcga agtttattta aagtggctaa aacacgaaaa
     3121 tatattgaat ttggagccct ttgcctcctg gcggtgggaa ttctctggtg gttcggccgt
     3181 acgctgaact ggactgaggt acgaactgcg gtcaaaaacg ctaatgttta catgctggtc
     3241 ggggcagcgc tcataatttc ctttgcctat gttatccggg catttcggtg gggagcattt
     3301 ctgcgaccgc tgggcgcctc acacttcagt catctttttg ccgccacaac aataggtttt
     3361 ggagctgttt ttctcgcagg acgggcaggt gaagttgtcc ggccggttgt attgcccatg
     3421 cgtgacccga gcgtacgccc gagtgcctcg ttcgtaacaa tactggtaga acgaatttac
     3481 gatttgtcag ccgttgtctt gatgtttgcg ataaatcttg tttggtttag gccacccgca
     3541 ggcttacagg ctgacttcaa tcgagttcga ttcgttggca tcgcattatt gacgatcgcc
     3601 attctgggtc tgattgtcct tgcctggttt cgaaaaaact cgagcagggt tgtcgaagcc
     3661 ttccagtcac tgtttgccca atggaccttt attccgcagc gctttgcgaa gttggtgatc
     3721 gcactagtgg cgcaacttgc gcgagccttg agcgtgcttg taaacgtgac agaactcatt
     3781 gagacagtgt tctggacagc cttgttatgg gtgggaatag cactggcgaa tcttctggta
     3841 attcgcgcgt ttggggtcca cttcgggttt tctgagactg tgtttgtgct gggctggtct
     3901 ctggttgctt cacttgtacc aacaccgggt ggagctgccg gcgcgtttca cgcagcaact
     3961 gctgccggac tattgtttct tggagtcgaa aaagagattg ccgcagccat ctcgattctg
     4021 atgcatttga tcgactttgg gcctgcactc atttttggat tggtttatgt aataaaaggg
     4081 gatttgaatt tttcacgttt gcgagctctg acttcacagg aagcagtcga agatgtcgtc
     4141 gaaaacgaag tactcattcc aggtgaaggt cataaggacc ggcaatcttt ggataatact
     4201 gctggtgagg ctttttaagg acccgattga gaggttcaac tgaatgaagt gtccattttg
     4261 tgcatatctc gaagataagg ttgtcgattc acgtgagtca cgtgaaggcg atgcaatacg
     4321 acgacgaaga gaatgtctca gatgtgagcg tcgttttact tcatacgaac gcatcgacga
     4381 aattccttat atggtggtaa agaaggatgg ccggcgtgaa aacttcgacc gcaataaggt
     4441 tatggctgga ttgttaagag cttgtgagaa acgtccggta gcgtcaacca aactggatag
     4501 catcgttaac gctatcgaaa aatatgtcca ggaatcgccc gaacgagagc gcccgacgag
     4561 taagattggt gagatgataa tgcggcgctt gaaagaactc gataaagttg cttatgttcg
     4621 gtttgcttct gtctatctgg aatttgagga tgtctcagaa tttatgacag agctcaaaca
     4681 tttagtcaga actcgcgcaa caggtacagc gggcagaaaa tgatcggacc gttcgcttcg
     4741 tttttgagag gtttgcgatg atcaaaccca ttcgggcgac aggattagag cgccttgaac
     4801 tacaacggct gagagatcgt gtcggccgcc tttacgcagc tttacaggaa gctactgaag
     4861 ctgaaaatcc aattgcctcg ggagcgtggg cgccaccagt cgatctcagt gaaactccgg
     4921 aagcaattgt gcttcgagtc gaattacccg ggtgtagcgc tgatcagatt atgattggcc
     4981 tatgcaatac gaagctcaga atctggggtg acaagaaaag gcgtccggcc cggcgcagga
     5041 tcatttctta tctttgctca gagcgaagct acggcaaatt taatcgcgtt gtccatttac
     5101 gttggactat tagtgttcgc gatgcggaag cggaacttac aaacggtatt ttgaacattc
     5161 gccttcccaa gattgaagac cgaaggggag gggaagtcat ggttccagta aaagaaacga
     5221 gccccgagta accctgattc aactagtctc attgtccaag gtggaggtcc gattatggca
     5281 ataccaaaag aaacagaatt tactgaagcg attgacacaa cagcctcgga ccagcagtta
     5341 caaatacctt ccgacttgcc ggtgctgcct ctccgagaca tcgttatcta tccgtttatg
     5401 atcgtgcccc tgtttgtgtc tcgcgagaaa tcgattcgcg cggttgacga ggcgctgggc
     5461 gagaaccgaa tgatcctcct tgcgtcgcaa aaagatctag acaaagagga acccacggct
     5521 gatgacctct attcaattgg aacagcggcc gtcatcatgc ggatgctgaa gctcccagat
     5581 gggcgaataa ggattctggt ccagggtctc gcccgcgcac aaatttcttc ggtcgacacc
     5641 acgggggaat atctgcgagc gcgtgtgaac gttattcagg agattttacc gccggaacgt
     5701 tcactcgaag tcgaggcttt ggtgcgcaac gttcgagcct cgatggagaa ggcgatcaac
     5761 ctcggaaaaa atatttctcc agaagtgatg gcgattatcg ctaacctcga cgatgccggt
     5821 cgccttgcag atttgtcggc atccaacctt gaattaaaag ttgaagatgc acagtcggtt
     5881 ctggatattg ccgacaccac cgcaagactg cgacgcgtta atgaccttct aaacaaagag
     5941 attgaggttt taactgtcca acaggaaatc aatacgcaag cgcgagccga tatcgatcgc
     6001 tcgcagcgcg agttttacct gcggcaacaa atgaaggcga tccaaagtga acttggtgaa
     6061 ggaaacgagc ttgctgagga gattgctcag tttagagaga agattgaagc ggcaaaaatg
     6121 ccgaaagccg ccgaagatga atcgcttcgt cagttgaaaa aacttgagcg gatgcatcct
     6181 gatgctgccg aaaccgcaac cctccgaaat tggatggaga tcatgacaga tcttccatgg
     6241 actcgatctt cagtcgataa cctcgagctg cagaaggctg aaaaagtctt aaatgaagat
     6301 cattacgggc tggagaaggt taaggatcga attcttgaag cgcttgccgt tcgtaaactc
     6361 aaagaaaaac ccaaaggtcc aatcctttgc cttgtaggac ctccgggtgt tgggaaaact
     6421 tctttaggtc gctctattgc cagagctttg gatcgaaagt ttgtacggct ctccctcggt
     6481 ggagtacatg acgaggcaga aatccgcggt caccgtcgca cgtacgtggg cgccatgcct
     6541 ggacgaataa tacaagcggt tcaacaagct ggaaccaata atcctttgat catgctcgat
     6601 gagatcgaca aggtcggcgc agattttcgt ggcgacccgt catcagcatt actcgaggtt
     6661 ctcgatcccg agcagaacaa cagttttcga gacaactacc tgggcgtcac ttttgatctc
     6721 tcgaacgtga tctttatgac gactgcaaat gtgttggaca ccattcaacc ggcgttgaga
     6781 gatcgaatgg aagtcatccg gctcgctggc tacacggaag aagagaaaag agagatcgcc
     6841 gttcgccatc tcttaccaaa acagatggac gagaacggta ttacttcgaa ggatctaaac
     6901 atctcacgaa cagcgctaat cgcgattatt cagcagtaca cccaggaagc aggtcttaga
     6961 cagcttgaac gtgaaatcgg ccgaatttgc cgcaaggttg ctcgtaagat cgctgaagga
     7021 cacaaaggaa caattcgcgt ttccttgaaa aatctccacg agtttctcgg cgctcccaaa
     7081 atcattcccg acgaggttct gaagaaggat cagatcggaa tagctaccgg tttagcatgg
     7141 acagctgttg gtggtgatgt gcttttcatt gaggctctcc gaatgaaagg caagggcggt
     7201 ttagtgctca ccggtcaact gggcgacgtc atgcgcgagt cggctcaagc agcctattct
     7261 tacgcaaagt ctcgcgctaa agaactcgat atcgccgaag aagatttcaa caactacgat
     7321 ttgcatatac acattcctga aggtgcaatt cccaaggatg gtccatctgc gggtatcacg
     7381 ctggcgactg cgatggtctc agcattgtct tcgcgtccga ttcgtcgaga cgttgcgatg
     7441 actggtgaaa taaccctgcg cggaaatgtt cttccgatcg gtggtgtcaa ggagaaggtg
     7501 ttggcagcgc ggcgagcccg ggtgtcgacg attattctgc cgcagca
//
LOCUS       DASSFP010000142         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_185419, whole genome shotgun sequence.
ACCESSION   DASSFP010000142 DASSFP010000000
VERSION     DASSFP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185419"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFH91_03080"
     CDS             complement(<1..702)
                     /locus_tag="VFH91_03080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6861994.1"
                     /translation="MNDASELTNSAALPKTSAQPAALAGTAETVGGKVGHYRWVICGL
                     LFFATTVNYVDRQVLGILSKDLQQAIGWTEVDYGNIVAAFNAAYAFGLVMAGRLMDRI
                     GTRKGYIAAIIWWSLAAMAHALARTPLGFGVARAALGVGEAGNFPAAIKTVAEWFPKK
                     ERAFATGIFNAGSNVGAIVAPLMVPWIAGHWGWQWAFIMTGAIGLIWLAFWIPLYGRP
                     EEHPRVSKAELAHIQS"
     gene            complement(732..1508)
                     /locus_tag="VFH91_03085"
     CDS             complement(732..1508)
                     /locus_tag="VFH91_03085"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET6861995.1"
                     /translation="MSYSQLELNGKVAVVIGGTSGIGLAIARGMAEAGADVVPSSRRL
                     EQTEAAAREIEERGRRSLRIPSDVADRGSLQNLLDECVKAFGKVDILVNSAGRTKRAP
                     TLDFSEEDWDSIIDTNLTGTLRAAQIFGRHMLEREYGSIINIASLSTFVALYEVAAYC
                     ASKAAVASLTKSLAIEWGQRGVRINAIAPGVFRTPLNENLLDETERGREFLVRTPMRR
                     FGNVEELAGAAIFLASDAASFVNGEVLTVDGGFLASGVNQ"
     gene            1876..2814
                     /locus_tag="VFH91_03090"
     CDS             1876..2814
                     /locus_tag="VFH91_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6861996.1"
                     /translation="MSATTRQIIGRRASANNLWLSHDGQSDSRCLESIQPQILPYEIL
                     PSEEEVGRAMFEEIESAAQNKDGDLVIILLGGRGAQALHRLLGRLAASNEKDWLLRRL
                     RVFTQDALAPLRMDNGLSFVRDFERLLGENFFRKIKSFRPIRTDTENLEDELKNYIFE
                     LESVGGIDIFFLGLGPEANAASHLAYIKPGSGATVADVAGIIPISQSILEHHITKFKA
                     GGANVHESDEAECRSAQKILTLGPAAILGAKRIVQSIVDARTAPAKRESYRELLSASI
                     SEDAITRAKQLDDNPGLWLRLHPNVRSLILPDVLEN"
     gene            2828..>4147
                     /locus_tag="VFH91_03095"
     CDS             2828..>4147
                     /locus_tag="VFH91_03095"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HET6861997.1"
                     /translation="MSSIEIPQAYLSEYAIIVDDADNVAVVKKATTAGLEITFPDGST
                     LEIRNSVPPGHRFATRDIPEGEFVLQFGQPIGTSLGIKRGEQITHGNMSNDVPIVRDL
                     SAGLETPPPEYFSISERRTFTGFRRSDGRVGTRNYVLIVPTSMCASHEASQISTIAEF
                     TLYSREKFPNVDGVVAIPHNKGCGCQDGSTIDVMLRTLSNYADHPNVGAVILIDLGCE
                     KTNLAQVERYLLKREKAFDKPVAKIGIQDVGGTQAAIQHGLKEVERLLPEVNQTVREE
                     CSVSDLVLGVKCGGSDGFSGISANPSLGRAADMLVKSGGTVLITEVPEFCGAEHLLAN
                     RAKDVETGRAVYRMVDWYKEYASKFGAVLNNNPSPGNIAGGLLNITIKSLGAVAKAGT
                     TRVEGVVEYAETPKNRGLNLMQGPGYDQESTPGLVAAGATVVVFTTGR"
BASE COUNT         1030 a         1061 c         1089 g          967 t
ORIGIN      
        1 gctctgaatg tgggccaact ccgctttcga cacgcgcggg tgttcttcag ggcgaccata
       61 caacgggatc cagaaggcga gccagatcaa tccaattgcc ccggtcatga taaaggccca
      121 ctgccagccc caatgaccgg cgatccacgg aaccatcaaa ggcgcgacaa tcgctccgac
      181 attcgatccc gcattgaaga tgccggtcgc aaaggcccgt tcttttttgg gaaaccactc
      241 ggcgacagtt ttaattgcag ccgggaaatt tccagcctca ccaactccaa gtgcagcgcg
      301 cgccacgccg aagccaaggg gcgtccgagc caacgcatgg gccatggctg ccaaactcca
      361 ccagatgatc gcagcgatgt agcctttccg agttccaatg cggtccatca atctgccggc
      421 catgaccaga ccaaacgcgt aggcggcgtt gaaggcggcg acgatgttgc cgtaatcgac
      481 ctctgtccat ccgatcgcct gttgcaagtc cttcgataaa atcccgagaa cctgacgatc
      541 gacataattc acggtggttg cgaagaagag cagtccacag atcacccaac gatagtgacc
      601 taccttgcct cctacggtct cggctgtacc agctaacgca gcaggttgcg cactcgtctt
      661 gggtagagct gcactgttgg tgagctcgct ggcatcgttc atttggttgt tctccgcaga
      721 aggattggaa gttactgatt tacgccactc gcgagaaagc ctccgtcaac agttagcacc
      781 tcgccattga cgaaacttgc agcatctgac gctaaaaaga ttgcggcgcc tgctaactct
      841 tcaacatttc cgaaacggcg catcggggtt cgaaccagga attcccgacc ccgctcggtc
      901 tcgtcgagca agttttcatt gagaggcgtg cgaaaaactc ctggcgcaat tgcatttatg
      961 cgcacaccgc gttgccccca ttcgattgcc aacgacttgg tcaatgacgc tacggcggct
     1021 ttgctggcgc agtaggccgc gacctcataa agagccacaa atgttgagag agatgcgatg
     1081 ttgatgatgc tgccgtactc gcgctccaac atgtgtcgtc caaaaatctg ggccgcccgt
     1141 aacgttccgg tcagatttgt atcgatgatg ctgtcccagt cttcctcaga gaagtccagg
     1201 gttggtgcgc gcttggttcg acctgcagag tttaccagaa tatcgacctt gccgaatgcc
     1261 ttaacacact cgtctagaag attttgtagc gatccgcggt cggccacatc ggacggaatt
     1321 ctgagcgatc ggcggccgcg ttcttctatt tctctggccg ctgcctctgt ctgctcgagt
     1381 cggcgagagg agggaacaac gtcagcgccc gcctcagcca ttccacgtgc aatggccagg
     1441 ccgatgccag aagttccacc tattacaacc gcaacttttc cattaagttc gagttgagaa
     1501 tagctcatat acttttttaa aatcctgtaa ctttagttgg agaatggacg ttttttgggc
     1561 agctatcata accgatgctg catttccgtg cttgagcttt tgctggcaga aactgaagcc
     1621 ggtcctgtcg ccagggaaag agtaaaggtt agaccacttt atcgcagcct ggaaatgtct
     1681 aacatcaagg agacggtaac gtcaagttcc gataaaggct tgtttccgcc tataaatagc
     1741 catgtttgct ggcgctgaaa gctggtagcc aggttgcttg tcttgtttag ccacgcatgt
     1801 taaagtcagg cttcgaactt cagattggtc aaaccagtca gcgtctccag tgagtaactt
     1861 cgtagtgaga ccatcgtgtc agcaactaca agacaaataa tcggtcgacg agcttcagcg
     1921 aacaatctat ggctcagtca cgacggccaa tcggactctc gctgtcttga gtcaatccaa
     1981 ccacagatcc ttccttacga gatattgcct tctgaagaag aagtcggccg agctatgttc
     2041 gaagaaattg agtcggccgc gcaaaacaag gatggggatc tcgttattat cctgttgggt
     2101 ggacgcggcg ctcaggcgct tcatcgattg ctgggtcgcc tggctgccag caatgaaaaa
     2161 gactggttgt tgcgtcgact ccgagtcttc acgcaggatg ctcttgcgcc attgagaatg
     2221 gacaacgggt tgagtttcgt acgtgatttc gagcgtttgt tgggcgagaa tttcttccgg
     2281 aagatcaaaa gcttccgtcc gattcgcact gacactgaaa atctggaaga tgagttaaag
     2341 aattatatct tcgagttgga atctgtcgga ggaattgaca tcttctttct gggacttggt
     2401 ccggaagcca atgcggcatc gcatctggca tatatcaaac caggttcagg agcaacggtg
     2461 gccgacgtcg ctggcatcat tccgatatcg cagagtatcc ttgaacacca catcacgaag
     2521 tttaaggccg gaggtgctaa cgtccacgag agtgatgaag ctgaatgccg gtcggcccaa
     2581 aagattttga cgcttgggcc agcagcaatt ttgggcgcca aacgaattgt ccaatcgatt
     2641 gttgatgcaa gaacagctcc ggcaaagcga gaaagttata gagaactctt gagtgcatcc
     2701 atttctgaag atgcgattac aagagctaag cagttggacg ataatccggg actctggtta
     2761 cgcttacacc ctaacgtcag atcgttgatc ctaccggacg ttctggaaaa ctagttgaaa
     2821 aacaaatatg tcgagtattg aaatacctca agcatatctc agcgaatacg cgatcatagt
     2881 ggacgacgcc gataacgtgg cagtcgtgaa aaaagcgacc acggccggac ttgaaataac
     2941 atttccggat ggctcgacgc ttgaaattcg caattcagtt ccaccgggcc atcggtttgc
     3001 caccagagac atacccgaag gcgaattcgt tctgcagttt ggtcaaccca taggtacatc
     3061 gctaggtatc aagcgcggtg agcagataac acatggaaac atgtctaacg atgtgcccat
     3121 cgtacgtgac ctgtcagcag ggcttgagac tccaccaccg gaatattttt ccatctctga
     3181 acgacgaact ttcacgggct ttcgtcgcag tgatggccgt gttggcaccc gcaattacgt
     3241 cttaattgtt ccgactagca tgtgtgcgag tcacgaggca tcgcaaattt ccacgattgc
     3301 tgagttcact ttatacagcc gggaaaagtt tcccaacgtc gacggagtag tcgcaattcc
     3361 gcataacaag ggatgcggat gccaggatgg ctcgacgatc gatgtgatgc tgcgcacgct
     3421 ttcgaattac gcggatcatc caaacgtggg tgcggtaatc ttgatcgatc tcggttgtga
     3481 aaagactaat ctggcccagg tcgaacgtta ccttctcaaa cgagaaaaag ccttcgataa
     3541 gccggtagca aaaatcggaa ttcaggatgt tggtgggact caagccgcta ttcaacacgg
     3601 tctgaaagaa gttgaacgcc tgttgcctga agtgaatcaa acagtgcgcg aagagtgttc
     3661 agtgagtgat ctcgtgcttg gggtgaagtg cggcggttct gacggattct ctggcatatc
     3721 agctaatcca tcattaggcc gagctgccga catgctggtg aagtctggag gcacggtcct
     3781 gatcacggaa gtaccagaat tttgcggcgc agagcatttg cttgcgaatc gggctaaaga
     3841 tgtcgagaca ggacgcgccg tctaccggat ggttgattgg tacaaagaat atgcctccaa
     3901 gttcggagca gttctaaata acaacccaag ccccggcaat atcgcaggtg gtctgctaaa
     3961 catcactatt aagtctcttg gcgctgtagc aaaagccggc accactcgtg tcgaaggcgt
     4021 agttgaatat gcagaaacgc cgaagaatcg cggtctgaat ttgatgcagg gaccgggtta
     4081 tgatcaagaa tcgacgcccg gactggtagc tgcgggagca actgtcgtgg tcttcaccac
     4141 cggtcga
//
LOCUS       DASSFP010000143         4915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_773299, whole genome shotgun sequence.
ACCESSION   DASSFP010000143 DASSFP010000000
VERSION     DASSFP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773299"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /gene="bshC"
                     /locus_tag="VFH91_03100"
     CDS             complement(<1..1559)
                     /gene="bshC"
                     /locus_tag="VFH91_03100"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HET6861998.1"
                     /translation="MSTAEAACYSTPEESGLRVETFSFEIIPHQSRLFLDYLKDPVKL
                     RTFYPSAIRFHHELQERVPEVLGAHRVDRDKVCDALQAMNSRWHAGSETLKNIKLLRD
                     SDCVAVVSGQQAGLFTGPLYTIYKALSAVKLAGCLRQRGTKAVPVFWIAAEDHDFEEV
                     AKVEVIGRDCQLKQVEVSTALHREGQPVGGVLLNDSIDEVVNQLFELLPNSEFAADMK
                     DLVKNSWRPGRGYVESFAMMMTTLLDQYGLIFLDPLDSELKKLAAPLYSKAAEKASEI
                     ASAVEIRSRELEAAGYHAQVLATANSFPLFLHDHEGRRHSVSRLDNGSYKAKDLDEEY
                     SAAALAELALQSPERFSPNVTLRAVVQDYLLPTIAYCGGAAEIAYFAQTAEVYRVLER
                     PATPILPRSSLTMIERHTGRVLERYELRLPDFFDGIDSVIKCVVEKHLSAGTAKGFDA
                     AEQNISNELDRLRAELQTLDPTLAGALDTGRKKIQYQLDGLRTRFIKAQMTRDEAAHR
                     QLQRAFDQLYP"
     gene            complement(1707..2630)
                     /locus_tag="VFH91_03105"
     CDS             complement(1707..2630)
                     /locus_tag="VFH91_03105"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET6861999.1"
                     /translation="MSDKQKILDSLDGILLPTTTPFTSNGEIDFKALQQNLAHWLGTG
                     ISGIVLMGSTGERVHLDEREYLSVVEVARHQVPSDSAFVVGAGQQSIRGTVAEIKRAA
                     AIGADSVLVITPSFYRSAITQEVLVSYYQAVADDSPVPVLLYSMPALTGIKIDPETIA
                     TLSAHQNIIGVKDSSNDVAGFRATVERCPSEFAVMTGNGTVLFDMLQAGATGGILAVG
                     CVVPDVCLEIFHSFRHAAIDEARALQSHLTPLASAVTTKYGIGGLKTAMDLKGYCGGS
                     VRAPLQSPGEEAKLEISSLLNAVESVIRSVP"
     gene            complement(2829..4139)
                     /locus_tag="VFH91_03110"
     CDS             complement(2829..4139)
                     /locus_tag="VFH91_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862000.1"
                     /translation="MKELQLNAGTQKHIFQPDGKVAVVLSNGSRTIKGSWRTEAQGTE
                     AKENLLRYNIDGASQTPVAVRYRFNEFNQLTAQIPAAANGGVDSETCAFLGQILIDDA
                     HDLVYSIITPDGVASANKITVYGNLHFTEDSNNLSIEMSDGSHAEIKGEKGLTGTAML
                     KAEKNLVQEFKADDLLRFIAFTRNDLNTSAVRVPKKAQIEFVGKWDVNPDSGQLVFHS
                     KITGDLTKPDVAIAFAGSVKAVSAGFAYFADKDGTKLAFNIKGQHRWNSANAKWELSL
                     GYSERKFQATFEGQIDKTTKDGQGFVLAGKMSLEHEAGKSTKIDLKVEGSYQFNKTNK
                     LTFVVNVQTSDGKLTYDLMVEGKFAFKGGKLSFQLRLNDKGTGGMKATIELAFKGNQD
                     SLIKTLALVLDITPDKVKLTFEFELRMKFVDGVLVKEPPKKLVA"
     gene            complement(4172..>4915)
                     /locus_tag="VFH91_03115"
     CDS             complement(4172..>4915)
                     /locus_tag="VFH91_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862001.1"
                     /translation="QFLASLVPFDESLLPRSRTQVTTLSLDVNTGLSHDFRQGKRAGL
                     GIFNLSMQSSMSRSFEAFGADYKFNKLRAIVSADILFGLSSPKDVFVRYNRVMSTSTH
                     GTPLFELARLGGPLTVRGLEEGEIIGRKLSADQFEFGVNALVLWHLFTRKRVATMLQR
                     DCLDESTSSLPFDVSNAYLKVFYDLGRVHDPDSFLEPVSETARGYGLALEMRQLGGKN
                     INLSIGYAYSPDSALHRSGTIYTGVSYTF"
BASE COUNT         1202 a         1283 c         1179 g         1251 t
ORIGIN      
        1 tttggatata actgatcgaa agcccgctga agctgcctgt gcgccgcctc atctcttgtc
       61 atctgggcct taatgaatct ggttctcaat ccgtccagct gatattgaat ttttttgcgg
      121 ccggtgtcga gtgcgcccgc caaagtagga tcaagcgttt gcaactcggc ccgcaaccga
      181 tccagctcgt tgctaatgtt ctgttccgca gcatcaaaac cctttgctgt gcccgcgctt
      241 aaatgtttct cgacgacaca cttgatcacg ctatcaatgc cgtcaaaaaa atctggaagt
      301 ctaagttcat atcgctcaag gacgcggccc gtatgtcgtt cgatcatcgt caaactagag
      361 cgcggtagaa ttggcgtcgc aggtcgttcc agtacccggt agacttcggc ggtttgtgcg
      421 aagtaggcaa tctcggccgc gccaccgcaa taagcaatcg tcggaagaag ataatcctgg
      481 accacggctc gcaaagttac gtttgggctg aatcgctccg gactctgcaa tgctaactcg
      541 gccaacgccg ccgccgagta ctcttcatcc aaatccttcg ctttgtaact accgttgtcg
      601 agacgtgaaa ctgaatggcg cctgccctca tggtcgtgta gaaaaagtgg aaatgaattg
      661 gcagttgcca gtacttgtgc gtgataaccg gcagcctcaa gttcgcgact gcgaatttca
      721 accgcactgg caatttcaga agctttctca gcggccttcg aataaagcgg cgcggccaac
      781 ttcttcaact ctgaatcaag tggatcgagg aagatcaagc catattgatc aaggagcgtc
      841 gtcatcatca ttgcgaagct ctcgacgtaa ccacgtccgg gccgccagga attcttcacg
      901 agatctttca tgtctgctgc gaactcggaa tttggcagca actcaaacaa ttgattcact
      961 acttcatcga ttgaatcatt gagaagcaca ccgccgacag gttgcccttc ccgatgcaat
     1021 gctgttgaaa cctcaacctg tttcagctga cagtcgcggc ctatcacttc aacctttgcg
     1081 acttcctcaa aatcatgatc ctccgcagca atccagaaca cgggaacagc tttggtgcct
     1141 ctttgtctaa gacatccggc aagcttcacc gccgagagcg ctttgtaaat ggtgtaaagc
     1201 ggaccggtaa aaagacctgc ctgctgacca gaaacgacgg ccacgcaatc tgaatcgcgt
     1261 aacaacttga tattctttaa agtctcactt ccagcgtgcc aacgactgtt cattgcttgc
     1321 agggcgtcgc agactttgtc gcgatcaacc cgatgagcac cgaggacttc gggcactcgt
     1381 tcctgtaact cgtggtgaaa gcggatggct gaaggataga aggttcgcag ttttacggga
     1441 tctttgaggt aatccaaaaa caaccgggat tgatgcggaa ttatttcaaa tgaaaaggtc
     1501 tcgacgcgca gaccggattc ttcaggggtg ctataacaag cggcttcagc tgtactcaag
     1561 ttctttggct cctgcgcgac attctacgca ggataacgag atttctgcta gcgcggttga
     1621 gttagagccg agaaacacca gtcaaaggca gggcacagag taggagttaa tatggtccct
     1681 ggtgcgctga gtggttggag acctggttat gggacggacc tgatgacgga ttcgacggcg
     1741 ttgaggagtg agctaatttc gagctttgct tcttcgcctg gagattgcaa cggagctcgc
     1801 accgaacctc cgcagtaacc cttaaggtcc atagcagtct tcagaccacc aataccgtac
     1861 ttcgtggtta cagctgacgc caatggagtc aagtgtgact gcagggctct tgcctcatca
     1921 atcgcggcat gcctaaagct atggaaaatc tccagacaaa cgtcaggcac aacacacccg
     1981 actgcgagaa tcccaccggt cgcgccagcc tgtaacatat cgaacagaac ggttccgttg
     2041 cctgtcatca cagcaaactc tgaagggcac cgttcgacag tggccctgaa accagcaaca
     2101 tcgttagaac tatctttaac tccgatgatg ttttgatgag cactaagtgt ggcaatcgtt
     2161 tcagggtcga tctttattcc ggtaagagcc ggcatgctgt aaagcagtac tggaacgggt
     2221 gagtcatccg ctactgcctg gtaatagctg actaatactt cctgagtgat ggcggagcgg
     2281 taaaacgaag gtgttattac aagaacggag tctgctccga tggcagcggc tcttttgatc
     2341 tctgcgacag ttcctcgaat gctctgttgg ccggcaccga cgacaaaagc gctatccgat
     2401 ggtacctggt gacgggctac ctccacgaca cttaagtatt cgcgttcatc gagatgtacg
     2461 cgctcgcctg tcgatcccat gagaactatc ccgctgattc cggttcccaa ccagtgggca
     2521 agattttgtt gaagggcttt gaagtctatt tcgccgttag aggtgaaagg agttgtcgtt
     2581 ggaagaagga ttccgtcgag actgtccaaa attttctgtt tgtcgctcaa gtcgacgatt
     2641 atattgacgc tccataaaaa ggatcaactg cctgccccgc ctctctctcc gcgtcactct
     2701 gcggttctct gcgtctctct gcgttgctta acacctaact catagactca caataggaac
     2761 agtgaaagca acgcagagag acgcagagga ccgcagagtg acgcagagat agaggatgac
     2821 tgaccgtctt aagctactaa cttctttggt ggctccttca ctaaaacgcc gtcgacgaat
     2881 ttcatccgca gctcgaactc gaaagttagc ttaactttgt caggagtgat atcgagcaca
     2941 agcgccagcg tttttatcaa actatcctgg tttcctttga acgcgagctc gatagtcgcc
     3001 ttcatgccgc cagttccttt gtcattcaga cgcaattgaa acgatagctt gccaccttta
     3061 aatgcgaact taccttcaac catcaaatcg taagttagct tcccatcgct cgtctgcaca
     3121 ttcactacaa aggtgagctt attagtcttg ttgaattggt aactgccttc aactttcaga
     3181 tcgatttttg tggattttcc ggcttcatgt tccagagaca ttttaccggc gagcacaaag
     3241 ccttgaccgt ctttcgtcgt cttgtctatt tggccttcga acgtcgcctg aaactttctc
     3301 tcggaatagc ccagcgacaa ctcccatttc gcattcgctg aattccagcg atgttgccct
     3361 ttaatgttaa aggctaactt cgtcccatcc ttgtcggcga aatacgcgaa gccagcagac
     3421 actgctttga ctgaaccggc gaaagcgatc gccacatcag gtttggtcaa gtcgcctgta
     3481 atcttgctgt ggaagaccaa ttgaccgcta tcaggattca cgtcccactt gccgacgaat
     3541 tcaatttggg cttttttggg aacgcgaact gccgaggtgt ttagatcgtt gcgggtaaag
     3601 gcgatgaatc gcagcaagtc gtccgccttg aattcctgga ccaaattctt ctcggccttc
     3661 agcattgcgg ttcctgtcaa ccctttttca cccttgattt ctgcgtgact gccatcactc
     3721 atctcgatcg agagattgtt gctgtcttcc gtaaagtgca aatttccata aacggtaatc
     3781 ttgttggctg aggcaacgcc atccggtgta atgatggaat agaccagatc gtgtgcatca
     3841 tcgatcagga tttggcccag gaaagcgcac gtttctgaat cgacaccgcc gttagctgcc
     3901 gccggaatct gggccgtcaa ctgattgaac tcgttgaacc gatatcgaac tgcaactgga
     3961 gtctgggacg ctccatctat gttatatcga agcaggtttt cttttgcctc ggtcccctgt
     4021 gcctcagtgc gccaacttcc ctttatggtc ctgcttccgt tggacaaaac aacggcaact
     4081 tttccgtccg gctgaaaaat atgcttctga gttcctgcgt tgagttgtag ttccttcatt
     4141 tcaggctcct tctcattcgt gagttcaata atcagaaggt gtatgaaaca ccagtgtaga
     4201 ttgttccgga cctatgaagc gcggagtcag gagagtacgc atacccgatg gatagattga
     4261 tgttcttgcc acccaactgc ctcatttcca gagcaagacc gtaacctctc gcggtttctg
     4321 aaactggctc taaaaacgag tccggatcat gcacgcggcc gaggtcataa aagactttca
     4381 aatatgcgtt gctgacatca aatggaagac tggacgttga ttcgtccaga caatcgcgct
     4441 gaagcattgt cgcgacgcgt ttacgagtga ataaatgcca gagcaccagc gcgttgactc
     4501 caaactcgaa ctgatcagct gaaagcttac gtccaataat ctcgccttcc tccaaacctc
     4561 gaaccgttag tgggccacct aatcgcgcta attcgaataa cggagttccg tgagtactag
     4621 tactcattac ccggttgtac ctgacgaaaa catctttcgg cgaagagagc ccaaataaaa
     4681 tgtcggcgct cacaatggct cgcaacttat tgaatttgta gtcagcccca aatgcttcga
     4741 acgaacgcga catactgctc tgcattgaaa gattaaatat acctaagcca gctctcttgc
     4801 cttgtctgaa atcgtgactg agaccggtat ttacgtccaa tgagagtgtt gtaacttgcg
     4861 ttcgcgagcg gggcagtaga ctttcatcga atgggacaag cgaggccaga aactg
//
LOCUS       DASSFP010000144         6411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_482497, whole genome shotgun sequence.
ACCESSION   DASSFP010000144 DASSFP010000000
VERSION     DASSFP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482497"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFH91_03120"
     CDS             complement(<1..725)
                     /locus_tag="VFH91_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HET6862002.1"
                     /translation="MRFWIDLANSPHVPFFRALTDRLLSMGHEVEITARDFAETIPLA
                     EAAGMLPKAIGRHGGRELSGKAGTLLTRAVALSRWGKAKKFDIALSHNSYSQILAARL
                     MRLRSVTLMDYEYQPANHLAFRFASRVIVPESFPSERLQRYGAGKHKVRHYHGTKEDV
                     YLAAFQTDQSFSAQLCKLGISPDNVLVLMRPPAHEALYHRFHNTLFDEALSKLLAIEN
                     VKVVLLPRTETQRATYQTRAHQNL"
     gene            complement(761..1774)
                     /locus_tag="VFH91_03125"
     CDS             complement(761..1774)
                     /locus_tag="VFH91_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6862003.1"
                     /translation="MIQVGVIGYGYWGPNLVRNFMAAPGSSVVAVADVREERLQPLRK
                     LYPSLKTSNDAGSVINDTTVDAVIIATPVSSHFELAMAALKAGKHVLVEKPLATTSKQ
                     ARQLIDFAAARNLVLLVDHTFVYTDAVRKMRELITTGALGEIYYYDAVRVNLGLFQHD
                     INVIWDLAIHDLSIMDFVLPDKPVAVSATGISHVPGQPENVAYITLFFANRQIAHVHV
                     NWLTPVKVRHTLIGGSEKMILYDDLEPSEKIKVYDKGITVTPRPEDVYKLLVSYRSGD
                     MYAPRLDNTEALQNEALHFIDCIENGTKPETDGHAGLRMVTMIEAAEKSLSGRGRLIE
                     LAL"
     gene            complement(1781..2257)
                     /locus_tag="VFH91_03130"
     CDS             complement(1781..2257)
                     /locus_tag="VFH91_03130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6862004.1"
                     /translation="MPIAADVRLGKDVRIFQPELVNLYGCSIGSETKIGAFVEIQKNA
                     KVGARCKISSHSFICEGVTVEDEVFIGHGVMFTNDLYPRAVNEDGTPQTESDWTVLTT
                     TVKKRASLGSNATVIAGVTIGANALVGAGSVVTQDVPDFAIVAGVPARVIGDVRER"
     gene            complement(2261..3457)
                     /locus_tag="VFH91_03135"
     CDS             complement(2261..3457)
                     /locus_tag="VFH91_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HET6862005.1"
                     /translation="MNDLSTISSGDLDAAYSQLLLWCRERDFAGHDPFDALNSKVFQL
                     SPLASSRNARLVFTQILKRSPSDFRNLARVPPQRNAKGIALFALAHLADYRWKKTIES
                     ERAARSLLADLQEMKLTNYTGAAWGYNFDWQSRNFFAPRGTATIVPTAFAGRAFIEAG
                     RALGDENYFKEARSICDFIVADLPRSVETKAEVCFSYAPETSTQIFNASLLAAEVLAG
                     VGAVTKEKELIELAERATRFVINHQKSDGSWVYGMDSAQSWIDNFHTAYVLFSLDRVI
                     KNSREMDEFRPALTRGYQFWKESFFLADGWPKYYHHHPYPVDVHAGAAAIVTFLELAG
                     LDSSATVSAEKIAEWLITNMRDEQGFFYYQRRRFYTVRKPYMRWTQAWMLYALARLRE
                     SRAIQS"
     gene            complement(3475..5403)
                     /gene="asnB"
                     /locus_tag="VFH91_03140"
     CDS             complement(3475..5403)
                     /gene="asnB"
                     /locus_tag="VFH91_03140"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6862006.1"
                     /translation="MCGITGWANLDSHVPPSPGGRDLLHSMCERMLHRGPDSEGLFVT
                     RGAALGMRRLAIIDLLTGEQPVFNEDRSVAVVLNGEIYNYREVRANLEERGHNFRSAS
                     DTEVLPHLYEEYGDEMVHELNGMFAFALWDSARQRLLIARDRFGEKPLYWGVFDNTLL
                     FASEPKVLLAHPSVKPSLNFQALRQYLSFDYVPAPLSIYEGVHKLPAAHRLVLQNGKV
                     TVEPYWKLSYKTNAKVPTEQEAAEHLRELLADSVRMRLVSDVPLGVLLSGGVDSSTVA
                     ALAVAASTDAVKTFSISFAESSFDESAYARGVAKFLGTDHHEERLSANLAANLVGEIG
                     SWMDEPFSDPSLVPTYLLSRFTRKHVTVALGGDGGDEIFAGYPMYAGHRWAEIYKRVP
                     QFLRAGMVEPLIRLLPVKTKNLSLDYKALKFVNGIKYDTVARHHVWFGSFTPEEQLQL
                     LSADALAITESDIYRQARELVEDCDSSDLVTRMQSLDTRLYLAEDILTKVDRASMAVS
                     LEVRAPFLDPRVAEFAASLPPHYKLRGQKTKYILKKSVDQLLPRFVTRRSKKGFGVPV
                     AEWLKVKLRPLARDLLSPERVRRAGVFNSAYVTKLLDEHERGVANHRKPLWTLLMFEL
                     WHESFVETPRRIETSVGS"
     gene            complement(5418..>6411)
                     /locus_tag="VFH91_03145"
     CDS             complement(5418..>6411)
                     /locus_tag="VFH91_03145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6862007.1"
                     /translation="LPSQYESPAVDSAPEISVFLPVFNEEPNLRPLHARLDQALKALN
                     RSAEVVYVDDGSRDGSLKILRELAQQDPRVRVVALRRNYGQTAAMAAGIDAARGKVLI
                     PMDADLQNDPADIARLLAKLDEGYDVVSGWRKNRKDKMVSRKIPSMLANRLISWIGGV
                     PLHDYGCSLKAYRRESLQEVRLYGEMHRFIPIYASWDGARVTEIPVEHHARTMGKSKY
                     GIERTLKVVFDLMTIKFMASYQTKPIYVFGSFGMLAFAISFLAGFYAVFLKIFHRADF
                     VQTPLPILTIVMFAVGIQFVLMGLLAEMLVRTYHESQAKPIYAVRERLGFPKVQ"
BASE COUNT         1657 a         1707 c         1549 g         1498 t
ORIGIN      
        1 agattctgat gtgctcgggt ttgataagtt gcccgctggg tctcggttct cggtaacaac
       61 actaccttta cgttctcaat tgccagcagt ttgcttaacg cttcatcgaa aagagtgttg
      121 tgaaaacgat gataaagcgc ctcatgagcg gggggtcgca taaggacgag cacattatcc
      181 gggctaatcc caagtttgca caactgcgca ctgaatgatt ggtccgtttg aaaggccgcg
      241 aggtatacgt cttccttggt gccatgatag tgacggacct tgtgcttccc tgctccgtaa
      301 cgctgcaacc tttcggaggg aaagctctcg ggaacgatca ctcgtgaagc aaaacgaaat
      361 gccagatgat tcgcgggctg atattcataa tccatcagcg tcacggaacg cagtctcatt
      421 aatcgagctg ccagaatttg agaataagaa ttgtgactta aggcgatatc gaattttttt
      481 gcctttcccc agcgcgatag ggccactgct cgagtcaaca aagtcccggc ttttccggac
      541 agttcacggc caccatgacg gccgatcgcc tttggcaaca tgcccgcagc ttccgcaaga
      601 ggaattgtct cagcaaagtc acgtgcagta atttccactt catggcccat tgagaggaga
      661 cgatctgtca gcgctctaaa gaaaggaacg tgtggagagt tagcgaggtc gatccaaaaa
      721 cgcattgcaa ctggcgctca gattttctta accaacggga ctacaatgcc aattcaatca
      781 aacggccgcg gccacttaga gacttttcag cagcctcgat catggtcacc atccgcaaac
      841 cagcatggcc atccgtttct ggtttggtgc cattctcaat acaatcaatg aagtgaagcg
      901 cctcgttctg aagagcttcg gtgttatcaa gtcgcggagc atacatatca ccggaccgat
      961 agctgacaag taacttgtaa acatcctcgg gccgtggcgt tactgtaata cctttatcgt
     1021 agactttgat cttctcgctg ggctcaagat cgtcatacag aatcatcttc tcgctaccac
     1081 cgatcagagt gtgcctgacc tttaccggag taagccagtt aacatgcacg tgcgcaatct
     1141 gcctgttcgc aaagaacaac gtgatgtacg caacgttctc aggttgaccg ggcacgtgac
     1201 tgattcccgt tgctgaaacg gcaaccggtt tgtcaggaag cacaaagtcc atgatcgaaa
     1261 ggtcatgtat tgcgagatcc cagatgacgt tgatatcgtg ctgaaagaga ccaagattca
     1321 cacgtaccgc atcgtagtag tagatctctc ccaacgcacc ggtagtaatc aactcgcgca
     1381 tcttccttac ggcgtcggta tacacaaagg tatggtccac taaaagcacc agattccgcg
     1441 cggcagcgaa atcgatcaat tggcgcgcct gcttcgaagt ggtggcgaga ggcttttcga
     1501 cgagaacatg tttgccggct ttcaaggcgg ccatcgccaa ctcaaagtgc gatgagacag
     1561 gcgtggcgat aattacagcg tcgacggtag tatcgttaat aacgctgcca gcatcgtttg
     1621 aagttttgag agagggatag agcttgcgta acggttggag tcgctcttcg cgaacgtctg
     1681 cgacagcgac gaccgatgag ccgggcgccg ccataaagtt ccgtaccaga tttggccccc
     1741 aatatccgta cccgatgaca cctacttgaa tcatttcaat ctatcgttca cgcacgtcgc
     1801 caatcacacg cgctggaaca ccggcaacta tcgcaaagtc aggaacgtct tgcgtcacaa
     1861 ccgaaccagc accgactaat gcattcgctc cgattgtgac tcctgcgatt actgtagcat
     1921 tacttccgag actggcccgt ttttttactg tcgtcgttaa cacggtccaa tcactttcag
     1981 tctggggagt tccgtcttca ttcactgcgc gaggatacag atcgttggta aacatcactc
     2041 cgtgtccgat gaaaacttca tcctcgactg ttacgccttc gcaaataaaa ctgtgcgatg
     2101 aaattttgca gcgagcgccc accttcgcat tcttctgaat ctcaacgaac gcgccgattt
     2161 ttgtttctga cccaatcgaa catccataga gattgacgag ctctggttga aagatccgca
     2221 catctttacc aagtcgaaca tcggctgcta ttggcatgat ttaactctga attgctcgac
     2281 tctctcgcag gcgggccaaa gcgtacaaca tccaggcctg agtccaacgc atatacggct
     2341 tgcgaacggt atagaagcgt cgtcgctgat aataaaagaa accctgttca tcacgcatat
     2401 ttgttatcag ccactcagca atcttttcag cactcacagt tgcactcgag tcaagaccag
     2461 ccaactccag gaaagtaaca atggcagctg caccggcgtg tacgtcaaca ggatacgggt
     2521 gatgatggta gtatttcggc catccgtccg caagaaagaa gctctctttc caaaactgat
     2581 acccgcgagt taatgcgggt cgaaactcat ccatctctct actgttcttg atgacacggt
     2641 caagggaaaa gagcacgtag gcggtgtgaa agttgtcgat ccacgactgt gctgaatcca
     2701 taccatagac ccatgagcca tctgattttt gatgattgat tacaaagcgc gttgcccgtt
     2761 cagcaagctc gatcaactct ttttctttgg ttactgcccc aacccccgcc aacacttcag
     2821 cggccagtaa gctggcatta aaaatttgcg tcgatgtttc cggcgcataa ctaaaacaga
     2881 cttccgcctt cgtttctacc gagcgaggta aatcggcaac gatgaagtcg caaatactcc
     2941 gggcttcttt aaagtaattc tcatcgccaa gtgcacgacc agcttcaata aaggctctac
     3001 cggcgaaagc tgtcggcaca atcgtcgccg tacctcgagg cgcgaaaaag tttcgactct
     3061 gccagtcgaa gttgtagccc caggccgcgc cggtgtaatt cgtcagcttc atctcctgca
     3121 agtctgccag gagtgatcga gctgcacgtt cagactcaat tgttttcttc caccggtaat
     3181 cggccaggtg ggccaacgca aacagggcaa tcccttttgc gtttcgctga ggcgggactc
     3241 gagcgagatt gcgaaaatca gacggagatc tttttagtat ttgagtgaaa accaggcgag
     3301 cattgcgaga agacgcaagc ggcgacagct gaaagacctt gctgttcagc gcgtcgaaag
     3361 gatcatgtcc ggcaaaatct cgttcgcgac accacaggag tagctgcgag tacgctgcat
     3421 caagatcgcc acttgagatt gttgaaagat cattcacgcg aaaatcgttt cacgttagct
     3481 tccgacggag gtctcaatcc ttctgggtgt ttcgacaaaa ctctcatgcc atagttcaaa
     3541 cattaataac gtccaaagag gcttacgatg atttgccacg ccgcgctcgt gctcatcaag
     3601 cagtttggtg acatatgcag aattgaagac tccggcacgt ctgacacgct ctggcgacag
     3661 aagatctcga gccagaggtc gcagttttac cttcaaccat tcagccaccg gcactccaaa
     3721 acctttctta cttcgcctgg tcacgaaacg aggtagcagc tggtccactg acttcttcag
     3781 gatgtacttc gtcttctggc cacgtagttt gtaatgcggt ggtagcgacg ccgcaaattc
     3841 tgctacgcga ggatccagaa atggcgctct aacttcaaga gagacggcca tgctggcccg
     3901 atcgactttt gtaaggatgt cctcggccag atatagccgc gtatcaagac tctgcatcct
     3961 cgtaacgagg tcagaactat cacaatcctc gaccagctcc cgggcctgcc ggtaaatatc
     4021 cgactcggta atcgctaaag catcagcaga aagtagttgc agttgttctt ccggagtaaa
     4081 cgatccgaac caaacatggt gtcgtgcgac agtgtcatac ttaataccgt ttacaaactt
     4141 aagggccttg taatcgagcg acagattttt ggtcttaacc ggtaataacc taattagcgg
     4201 ttcaaccatt ccagcacgca gaaactgagg aactctctta tagatctcgg cccagcgatg
     4261 gcccgcgtac attggatatc cggcaaagat ctcatcgcca ccgtctccgc cgagtgcgac
     4321 tgtcacatgt ttgcgcgtga atcgcgaaag caaataagtc ggaaccaggg aaggatcgct
     4381 gaatggctcg tccatccatg aaccgatttc gccgaccaga tttgcagcaa gatttgcact
     4441 caaacgctct tcgtgatggt ccgtacccag aaactttgcg acgcctcgag cgtatgccga
     4501 ctcgtcgaac gacgactcag caaatgaaat cgagaatgtt ttgacagcgt cagtcgaggc
     4561 agcgacagcc agagcggcca cggtggaaga gtcgacgcca ccagagagta agacaccaag
     4621 aggcacgtcg gatacaagcc gcatgcgaac ggaatcagct aacagctcac gtaggtgttc
     4681 ggcagcttct tgttcagtag gtacttttgc attggtcttg tagctgagtt tccaatacgg
     4741 ttcgacagta acttttccat tttgcagaac gagacggtgt gcagcaggaa gcttgtgtac
     4801 accctcgtag attgaaagcg gcgccggcac ataatcaaat gacaggtact gtcgcagggc
     4861 ttgaaagtta agtgatggtt ttacggatgg atgcgcgagc agaactttcg gttcagaagc
     4921 gaagagtaag gtgttatcaa acacacccca gtacaaaggc ttctccccaa atcgatcgcg
     4981 agcgattaac aatcgctgac gcgcactgtc ccagagcgcg aacgcaaaca ttccgttgag
     5041 ttcatggacc atttcgtcgc cgtactcttc gtagagatgg ggcaggactt cagtgtcgga
     5101 cgcgctgcga aagttatgtc cacgctcttc aagattcgct cgtacttcac ggtagttgta
     5161 gatttcgccg ttaaggacca ccgccacact tctatcttcg ttgaaaaccg gctgttcacc
     5221 agtcagcaga tcgataatcg cgagccgtcg cattccgagc gccgcaccgc gcgtgacaaa
     5281 gagaccttct gagtctggtc cacgatgcag cattcgctcg cacatcgaat gcaacaagtc
     5341 tctgcctccg ggagatggcg gcacatgcga atcgaggttg gcccaaccgg taattccaca
     5401 cataaggctt aaaatcttta ctgcacttta ggaaacccga gtctctcgcg caccgcataa
     5461 atcggcttgg cttgagactc atgataagta cggaccagca tctcagcgag caagcccatc
     5521 aagacaaact ggatgccgac tgcaaacatg acgatcgtca agatcggcaa gggagtttgt
     5581 acaaaatcag cgcgatggaa aattttcaag aagaccgcat agaagccggc cagaaaagag
     5641 atcgcaaatg ccagcattcc aaacgagcca aacacgtaaa tcggttttgt ttgatatgac
     5701 gccatgaatt tgatcgtcat caagtcgaaa acgactttca acgtacgctc gattccatac
     5761 ttcgacttgc ccatggtgcg agcgtgatgc tcgaccggga tctccgtcac tctcgcgccg
     5821 tcccatgaag cgtaaatagg aataaagcga tgcatctcgc catacaaacg aacctcctga
     5881 agcgactcgc ggcgataagc tttcagcgag catccgtaat catgaagagg tactccgcca
     5941 atccatgaga tcaatcgatt agcaagcatc gacgggatct tgcgcgacac cattttgtct
     6001 ttacgatttt tgcgccaacc tgaaaccacg tcataacctt cgtcgagctt tgccaacagt
     6061 ctcgcaatgt cagccggatc gttttgcaag tccgcatcca tcggaattag gaccttacct
     6121 cgcgctgcat cgatacctgc tgccattgcg gctgtttgac cataattcct tctgagagcg
     6181 acaacgcgta cgcgcggatc ttgctgtgca agttcacgca agatcttcaa actcccatcc
     6241 ctcgatccgt catcgacata aacaacctct gctgaacgat tgagagcctt tagcgcctgg
     6301 tccaacctgg catgcaatgg gcgaagatta ggttcctcat tgaagacagg aagaaaaacg
     6361 gagatctccg gagccgagtc aaccgccggc gactcgtatt gcgatggcaa a
//
LOCUS       DASSFP010000145         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1224304, whole genome shotgun sequence.
ACCESSION   DASSFP010000145 DASSFP010000000
VERSION     DASSFP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224304"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1821)
                     /locus_tag="VFH91_03150"
     CDS             complement(208..1821)
                     /locus_tag="VFH91_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HET6862008.1"
                     /translation="MSYQKVALRLAVIVATLAFSSPAVIPQDQQDPTNKPRNVKPELK
                     KAYKDWLDKDVTYIITDEERKAFKKLATDDERERFIEEFWRRRDPDPDTDENEFKEEY
                     YERIAYANEHFSSGIPGWKTDRGRIWIMYGKPDERETHPMGGSYERPSYEGGGSTTTY
                     PFEIWFYRYLAGVGSGVEIEFVDPTGSGEYRIARNPNEKDAMLMVPGAGLTLSEQLGL
                     SDKADRIAGVGGIGNPQYQREQDSPFSRLQLLADLSRAPQVKFNDLATAVNTGVVEEN
                     PLNFELRVDFFRQSDERVITAFTVQAENRDLVFQDSGGLQQARMNIFGKITSVAGRRI
                     QIFEDPVITTATPEELTEAKDRKSAYQKAVPLAPGTYKVDVIVRDVSSGATGIKNLGF
                     TVPKYDQEKLSTSTMILAAKLEGLNDQPAVGQFVIGNAKVIPNVSGQYHRGQPVGVYL
                     QVYNAGIDQTTLRPSVDVEYALIKDGKEVGKQPEDWRGMSDSGQRLTLAKLIDTSKLP
                     NGEYEVAIRIKDRVTGQSLSPSQKFTVIQ"
     gene            complement(1993..>2439)
                     /locus_tag="VFH91_03155"
     CDS             complement(1993..>2439)
                     /locus_tag="VFH91_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET6862009.1"
                     /translation="SPGLYPHERAYVTPFIALSSISFLLGAAFAYYVIFPPAAKYLLG
                     LGSDFRLLLKADDYFDFIIIVMLGMGVVFQMPAITYVLARIGLVTAGFMVRIWKTALI
                     VILVAAAVLSPTNDIPNMLLFAAPMLVLYVVSIFVAWIFNKPRTVS"
BASE COUNT          613 a          597 c          604 g          625 t
ORIGIN      
        1 ctcacaaacg cgacaactgt gattgagatc agaagacctc gaagcatgga gagaaacatt
       61 ttggtgtcct ttcagacgct taggtcgcat taacgcaatt ggccagggga gagaaggcca
      121 ggcgagttgg aggttaaatc aaaatacgac gctccctgtt aagaaagcgt cgtatagtca
      181 agcaacagag agattgagtg atcgtcttta ctgaatgaca gtaaacttct gcgatggtga
      241 aagtgactga cctgttacgc ggtccttaat tcgaatggcg acctcatact caccgttcgg
      301 cagcttgctt gtgtcgatca attttgcaag cgttagtctt tgaccggagt cactcatacc
      361 gcgccagtct tccggttgtt tcccaacttc cttgccatct ttaatcagcg catactcgac
      421 atcgactgat ggacgtaacg tggtttggtc gataccggcg ttataaacct gcaggtaaac
      481 gccgactggc tgaccccggt gatattgacc ggaaacgttg ggaattacct tcgcattacc
      541 aattacaaac tggcccacgg ctggttgatc attcaaacct tccaatttgg cggccaggat
      601 cattgttgaa gttgagagtt tttcctgatc gtatttagga actgtgaagc caagattctt
      661 tatccctgtg gcgcctgagc ttacgtccct aacgatgacg tccactttgt aagtgccagg
      721 ggcgagtggg acggccttct gataagcaga ttttctatct ttcgcttcag tgagttcttc
      781 cggagttgcg gtagtgatga ccggatcctc aaagatctga atgcgacggc ctgccactga
      841 agtgatcttg ccaaagatat tcatccttgc ctgttggaga ccaccactat cctgaaatac
      901 aaggtcgcga ttttctgcct gaaccgtgaa cgcagtgatt actctctcgt ctgattggcg
      961 gaagaaatca actcgcaact cgaagttaag tgggttctct tcaaccacac cggtgttgac
     1021 ggctgttgcc aggtcattga attttacctg cggcgctcgg ctcaaatcag cgagcaattg
     1081 cagtcgagag aatggactat cctgttcacg ttggtactga ggattgccga tgccgcccac
     1141 gcccgcgatg cggtcggctt tatctgagag tcctaattgt tctgacaatg taaggccggc
     1201 gcctgggacc atcagcatcg catctttttc atttggattt cgagcgattc gatattcgcc
     1261 gctgcccgtt gggtcaacga attcgatttc cacgcccgaa cctacgcctg cgaggtaacg
     1321 atagaaccag atctcgaacg gataggttgt ggtcgagccg cctccttcgt aactgggtct
     1381 ttcgtaagag ccgcccatcg gatgagtctc acgctcatcg ggcttgccgt acatgatcca
     1441 gatgcgacct cggtcagttt tccaaccggg aataccagaa ctgaagtgct cattcgcgta
     1501 ggctattcgc tcgtaatact cttccttaaa ctcattttca tcggtgtccg gatctggatc
     1561 gcggcgacgc caaaactctt cgatgaatcg ctccctctcg tcatcggtcg ccagtttttt
     1621 gaatgcctta cgctcttcgt ccgtaataat gtatgtaacg tctttgtcta accaatcttt
     1681 gtaagctttc tttagctcag gtttgacgtt gcggggtttg ttcgttggat cttgctggtc
     1741 ttgcggtatt actgcaggac tcgaaaaagc gagggtggcc acgatcacag ccagccgaag
     1801 agcgactttc tggtaagaca ttccaaaact cctaataatg cttttgactt taattcgagc
     1861 agctacagat tgattccgga taccggtcga agaccgagga ttctaagctt gtgccaagaa
     1921 cacagtcaag atgccttgga agaagccttt agttgcccga aatgactcaa aaacatgatg
     1981 ttaatggccc tgttagctta cggttcgggg cttgttgaag atccaggcca cgaatattga
     2041 aaccacatac aaaaccagca ttggggccgc aaacaacagc atattgggga tatcgttggt
     2101 tggggacaaa actgccgcag ccaccaggat aacaattaag gcagttttcc aaatgcggac
     2161 cataaaacca gcggtcacca aaccgatgcg agccagaaca taagtaattg ccggcatctg
     2221 aaagacgacc cccattccta acattacaat gatgatgaag tcgaagtagt cgtcggcttt
     2281 cagcagcaat cgaaagtctg aacccaaccc cagcaagtat ttggctgctg gaggaaagat
     2341 gacgtaataa gcaaaagcgg cacccagtaa aaaggaaatt gaggacaacg cgatgaaggg
     2401 tgttacatat gcccgttcat gcgggtaaag gcccggcga
//
LOCUS       DASSFP010000146         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_774429, whole genome shotgun sequence.
ACCESSION   DASSFP010000146 DASSFP010000000
VERSION     DASSFP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774429"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1280)
                     /locus_tag="VFH91_03160"
     CDS             complement(165..1280)
                     /locus_tag="VFH91_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6862010.1"
                     /translation="METDIYMSEYQFNRRDFLRGLSVTTAGLLIRPDLLAQTGSRKLG
                     IALLGLGGYATGQLGPALRQTKLCELVAVVTGHPEKGEKWAADYNLKRENIYSYESFD
                     RIADNKSVDIVYVVTPPALHPEFVIRAARAGKHVITEKPMATSVADCDRMIGACRKAK
                     VQLGVGYRMHYDLFRKELMRLAKTQEFGPFMKMSGEFSFVMNQHQWRVEKKLAGGGAM
                     MDLGIYVVQSASMAANAWPISVTAKEDPKQRPDFFNEVEETIRWTMNFPNGATCDAVT
                     SFNRSGNHFRAEGAKGWFELTKNAFNYNGAVGQTSRGALNFDWINQQAAQMDDFADCV
                     MTGRKTPVPGEMGKAHMAIITAVYEATRTGKTVKVNI"
     gene            complement(1283..2308)
                     /locus_tag="VFH91_03165"
     CDS             complement(1283..2308)
                     /locus_tag="VFH91_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6862011.1"
                     /translation="MFRIGILGGGGISETHARAAQAVGGIEIVAFAGRNVESLRRLAA
                     KFGGVPYTNLVEFFRHKPMDAVIIGSPSALHSEQGIAAAERGLHVLVEKPIDVTVERA
                     DALIAACERADVKLAVCYQDRFAPDSVRLKQFIDRNGLGKILLVTSHVKWYRPPEYYS
                     TSGWRGKPEFDGGGALMNQGVHTIDLLLWLLGDVKSVFALSRTSLHDIESEDTLVATI
                     EFANGAVGTLEAATSAFPGYERRVEITGSEGTIIFKHDRIVAADLRSASAFPGGLSDQ
                     QNDTNRSANSPIVSDVSGHKSIIQDFLHAIRSNTVPRCDGREGRRSVALVQAIYKSAE
                     LGKPLNL"
     gene            complement(2321..>2616)
                     /locus_tag="VFH91_03170"
     CDS             complement(2321..>2616)
                     /locus_tag="VFH91_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6862012.1"
                     /translation="WETYVKISYPFLHADRIRTPTLFLGGERDFNVPVQGSQQMYQAL
                     RSLGIDTQLIIYPNENHGIQRPSYQRDRMERYLAWYDKYVKKLTAPSSTSTAK"
BASE COUNT          615 a          713 c          636 g          652 t
ORIGIN      
        1 ttgcatcact cgcgaggaac ccaagctccg ctcggatatt cgactcgaga attgcatccg
       61 aagacttgag ccgttgagct agagtcagtg ggccattggc aaataaaagg aggaagatta
      121 gtgctaatga ttttgttccc ttacgtgttg gcatatttga tgattcagat gttcacctta
      181 actgttttgc cggtgcgcgt agcctcgtaa actgcggtga tgatcgccat gtgggcctta
      241 cccatctcac ccggcaccgg cgtctttctt ccggtcatta cacaatcggc aaaatcatcc
      301 atctgggcag cctgctgatt aatccaatca aagttcaagg ctccacggct ggtttgacca
      361 actgcgccgt tgtagttgaa cgcatttttc gtcaattcga accaaccctt ggcaccttcc
      421 gcacgaaaat gatttcccga acggttgaag ctggttactg cgtcgcacgt cgcgccgttc
      481 ggaaaattca tggtccagcg aatagtttct tcgacttcgt tgaaaaaatc cggccgttgt
      541 ttcggatctt ctttggcagt aactgaaatc ggccacgcat tcgctgccat cgacgcactc
      601 tgtaccacat atatgccaag gtccatcatt gctccaccac cggcgagctt cttttccacg
      661 cgccactgat gttgattcat tacaaaacta aactcgcccg acattttcat gaaaggacca
      721 aactcttgag tcttcgccaa tcgcataagc tcttttcgga acagatcgta gtgcatgcga
      781 taccccactc ccagttgaac tttggctttt cggcaagctc cgatcatgcg atcgcagtct
      841 gcgaccgaag tggccatcgg tttttccgtg atgacatgct taccagcgcg tgcagcgcga
      901 atcacaaact ccgggtggag tgccggcggt gtaactacgt acacgatatc gacactctta
      961 ttatcggcaa ttcggtcgaa tgactcgtac gagtagatat tttctcgttt tagattgtag
     1021 tcggccgccc acttttcacc tttctcagga tggccggtaa ctacagcaac tagttcgcag
     1081 agttttgttt gacgtaatgc tggtccaagt tgtccggttg cataaccacc tagcccgaga
     1141 agtgcgatac caagtttacg gctgccagtt tgggccaaaa ggtccggacg aataagcaaa
     1201 cccgcggtcg taacggaaag cccgcgcagg aagtcgcggc gattaaattg atactcgctc
     1261 atgtaaatgt ccgtttccat ttctaaagat ttagtggctt gccaagttct gcagatttgt
     1321 agatagcctg gaccaacgca acactgcgcc ggccttctcg accgtcgcag cgcggaaccg
     1381 tatttgacct gatggcgtga agaaagtcct ggattatgct cttgtgaccg ctgacgtctg
     1441 aaacaatagg tgagttggcg ctgcggtttg tgtcgttttg ctgatccgac agaccacccg
     1501 ggaacgcaga agcagacctc aagtccgcag cgacaatccg atcatgtttg aagatgattg
     1561 tgccttctga gccagttatt tccacgcggc gctcatagcc gggaaatgct gaagtggcgg
     1621 cctccaaagt tccgaccgcc ccattcgcga attctattgt tgctaccagc gtatcctcag
     1681 attcgatgtc gtgaagactc gtacgcgaca atgcgaaaac actcttcaca tctccgagca
     1741 accacaaaag aaggtcgatg gtgtggacgc cctggttcat caaagcgccg ccaccatcaa
     1801 actcgggctt tcctctccag cctgacgtcg agtaatactc aggcggtcga taccatttaa
     1861 cgtgcgaggt cacaagcaag attttgccga gaccgttgcg atcgataaat tgcttcagcc
     1921 gtacactatc cggcgcaaat cgatcctggt aacagaccgc cagctttaca tctgcacgtt
     1981 cacacgcggc gattaaagca tcagctcgct caacggttac atcgattggc ttttcaacaa
     2041 gtacgtgtaa tccgcgttcc gcagcggcaa ttccttgctc ggagtgcaaa gccgaagggc
     2101 tgccgatgat aaccgcatcc atcggcttgt gcctgaagaa ctccaccaga tttgtgtagg
     2161 gaactccgcc aaacttagct gccagtcgac gcaggctttc gacgttgcga ccggcgaacg
     2221 caacaatctc aattccgccg actgcctgcg ccgctcgtgc gtgagtttcg ctaatacctc
     2281 ccccaccaag aatgccaatg cggaacatgc gtcagctttt ctattttgcg gtggaagtgc
     2341 tcgacggagc agtcagtttt ttaacgtact tgtcgtacca ggccagatat ctctccattc
     2401 gatcacgctg ataactgggc cgttgaatcc catggttctc attcggataa atgatgagtt
     2461 gcgtgtcgat acccaaactg cgcagggcct gatacatctg ctgactacct tgcaccggaa
     2521 cattgaagtc gcgctcaccg ccaagaaaca gggttggagt gcgaatgcga tcagcgtgta
     2581 ggaacgggta cgaaatcttg acgtaagtct cccagg
//
LOCUS       DASSFP010000147         4668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_186931, whole genome shotgun sequence.
ACCESSION   DASSFP010000147 DASSFP010000000
VERSION     DASSFP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186931"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFH91_03175"
     CDS             complement(<1..462)
                     /locus_tag="VFH91_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862013.1"
                     /translation="MNSNTKLKTQPLDSAKAETKPLSIDLLGQTFPVEFSSLVKVDVY
                     GLSHRGLVRPNNEDHFVVVRGGRELEAIMGNLPADVVSRRFDEVFYGMAVADGLGGEV
                     CGEIASRRALESLLSMVLHTPDWMLRLGKPEINEVMWRMADRFIRVHAALLH"
     gene            complement(481..1188)
                     /gene="aqpZ"
                     /locus_tag="VFH91_03180"
     CDS             complement(481..1188)
                     /gene="aqpZ"
                     /locus_tag="VFH91_03180"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HET6862014.1"
                     /translation="MSLAKKVTAEFLGTFWLVLGGCGSAVLAAAFPNVGIGLLGVALA
                     FGLTVVTMAYAVGHISGGHFNPAVTAGLCVAKRIPASLVIPYIVAQVIGAVVAAAVLY
                     LIASGHVGFDVSAGFAANGYGDHSPGKYTLVSAMVSELVMTFMFLIVILGSTDSRAPA
                     GMAPLAIGLSLTIIHLVSIPVTNTSVNPARSTGQALFVGGWALVQLWLFWLAPLVGGS
                     LAGLVYNWLGSNDDVKT"
     gene            1684..2373
                     /locus_tag="VFH91_03185"
     CDS             1684..2373
                     /locus_tag="VFH91_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862015.1"
                     /translation="MSNMKLRRTRIFVSGLILVLASGTLIPLSAQTKKRRAPVYHTVY
                     ANQVMRVRLNDELDSEKARVGDSFTATLVDPVYSRNGVLLAPQGSTVRGHVTNVVRAQ
                     KDGKPATLDVSFVALRLPNGIGRTISGSLTDLESSTGNSNNEGTVTAHKTSKRNVKFI
                     GGGAAGGAVIGAIAGGGKGLAIGAGAGALAGLIGGKLKKGHEVKVKSGTEFGVVLNRN
                     FSLPAYKPTAG"
     gene            2460..2723
                     /locus_tag="VFH91_03190"
     CDS             2460..2723
                     /locus_tag="VFH91_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET6862016.1"
                     /translation="MFSFLWWLIVGLIVGALARLIMPGRDAMGTLATMLLGIAGSILG
                     GLVSWAIWGTDNGRFQPAGFILSLIGAILLLGLYRMAKSRSAV"
     gene            2752..3654
                     /locus_tag="VFH91_03195"
     CDS             2752..3654
                     /locus_tag="VFH91_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET6862017.1"
                     /translation="MPELPDIVVYIEALEKRILGETLERVQVGSPFLLRTAVPPLATA
                     ENREVVSLRRLGKRICIGLEGDLWLVLHLMIAGRLHWFPKGTRAAKPRSQASLQFSSG
                     ILILTEAGSQKRASLHLIQGEAGLRQLDPGGLEVLDATLDQFSNRLLARNHTLKRALT
                     DPRIFSGIGNAYSDEILFEAQLSPILQTQKMTPEEIKTLYDATRKSLNEWVERLRAES
                     RDKFPAKVTAFRADMATHGRFGKPCPRCGGKIQRIRYAANETNYCPTCQTGGKLLADR
                     ALSRLLKADWPKTQEEFESLTAAS"
     gene            complement(3657..4130)
                     /gene="ybaK"
                     /locus_tag="VFH91_03200"
     CDS             complement(3657..4130)
                     /gene="ybaK"
                     /locus_tag="VFH91_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HET6862018.1"
                     /translation="MHKTNAARLLDQLKIQYDLREYDVDPDDLRAETVAAKIGLPAEQ
                     VFKTLLARGDRAGLCFAVIPANAELDLKALAASSGDRKIHLVPMKELLTLTGYIRGGV
                     TALAGKKDYPVYIDETIELFDLISISAGIRGVQILLAPADYVKATKGRVVAIAGD"
     gene            complement(4176..4493)
                     /locus_tag="VFH91_03205"
     CDS             complement(4176..4493)
                     /locus_tag="VFH91_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862019.1"
                     /translation="MSDEKLLALEGDDFSVFNQKERLVIELADAMVAAPANISDDLYA
                     RLQSEFSEEQLLELSAQLAFENYRARLNRVFNVPSDELYHSGSSNSKHLKTETPLARS
                     SAS"
BASE COUNT         1227 a         1233 c         1168 g         1040 t
ORIGIN      
        1 atgaagcaaa gcagcgtgaa cacgtatgaa tcgatcagcc atgcgccaca tcacttcatt
       61 tatttctggt ttaccaagtc gcaacatcca gtcaggcgta tgcaagacca tactcagcaa
      121 cgactcgagt gctcgcctgc tggcaatctc accacagact tcgcctccca gaccgtcagc
      181 cactgccatg ccgtagaaga cttcgtcaaa gcgacgagag acaacgtcgg caggcaggtt
      241 gcccataata gcttccaact ctcgtccacc tcggacaact acgaaatgat cttcattatt
      301 gggacgaacc agaccacgat gagatagacc atagacatca actttcacta gcgaagaaaa
      361 ctccaccgga aaagtttggc ccaggagatc aatgctcagc ggcttagttt ccgctttcgc
      421 actgtcgagc ggctgggttt tcagtttcgt attgctgttc actttcaact cccttgaagg
      481 ctacgttttc acgtcatcat tggagcccaa ccaattgtag accagtccag ccagtgagcc
      541 accaaccagc ggcgctagcc agaatagcca cagttggacc agggcccatc caccaacgaa
      601 taacgcctgt ccggtactgc gagccggatt aaccgaagtg ttggtaactg gaatactgac
      661 gaggtgaatt atggttaacg acaatccaat cgcgaggggg gccattcctg cgggcgctcg
      721 tgagtctgtc gagccaagaa tgacaatcaa gaacataaat gtcatgacca actcactaac
      781 catcgcactc acaagcgtgt atttaccggg agaatgatct ccgtagccgt tggcggcaaa
      841 tcctgccgac acatcaaacc caacgtgacc gctcgcgatg aggtacaaaa cggcagcagc
      901 cactactgct ccgattacct gcgcgactat gtacgggatg acgagcgagg ctgggatgcg
      961 tttagcgacg cataagccag cagtgacggc tggattgaag tgtccacctg aaatgtgacc
     1021 gacggcataa gccattgtta caacggttag accaaaagcg agggcgacgc ctaatagacc
     1081 aattcctaca ttcggaaaag cagcagccaa caccgcactg ccacaaccac ccagaacgag
     1141 ccaaaatgtt cctagaaact cagcggtaac ttttttcgct agactcattg gtttgccttt
     1201 cgagaacgga acgacgataa ggcaatgccg gtgaccgttc taactgctgt cgagtggtag
     1261 aacaaaccgg tacgagaccg gacgaattcg gcaaggggcg agatttttgg aggggtgcag
     1321 tttaccctca aatccgaccg attgaaatag caaaaatgcg aaattctctg cactcctctg
     1381 tggggactcc gcgtgtctcg gcggtttagt aacatccgag cctaagcgac gcagagggac
     1441 gctgagatct cgcagagagc cgcagcgatt actcaagtcc tcctgcttag caagatccaa
     1501 aaaagtcgat ccgtgtctag cgaggtgttt ggaaagtttg tcacgggaag gcggtatgct
     1561 gcccaaatgt ctgaaaagtt attccactaa acgctgactt tgggtacact attcgcaccg
     1621 caagacagat tacccacaga aaaggactcg acccgtcggt cgaatccaaa cgaggagaag
     1681 cagatgagca acatgaaact tcgtcgaacc cggatctttg tttccggact aatcctggtg
     1741 cttgcaagcg ggacactcat ccccttgtca gcccagacca agaagagacg agctccggtc
     1801 tatcacacag tctatgcaaa ccaggtcatg cgagtcagac tcaatgacga gttggattcc
     1861 gagaaagctc gcgttggcga tagcttcact gcaaccctcg tcgatccagt ttattctcgt
     1921 aacggcgtgt tactggctcc ccagggcagt acagtgcgag gccacgtaac aaacgtggtg
     1981 cgcgctcaga aggacggaaa gcctgcgaca ctcgacgttt catttgtcgc gctccgattg
     2041 cccaatggta tcggccgaac aatcagcggc tctctgaccg acctggagtc atccactgga
     2101 aacagtaaca atgaaggtac cgtcactgcg cacaagacca gcaagcgcaa tgtcaagttt
     2161 atcggtggcg gagcagcggg aggcgcagtc attggtgcga tcgcgggtgg tggaaaaggg
     2221 ttggcaatcg gtgccggtgc cggagcgtta gccggactga ttggtggtaa acttaagaag
     2281 ggtcacgaag taaaagttaa gtccggtacc gagttcggag ttgttctgaa ccgaaacttt
     2341 tctttacctg cttacaaacc gaccgcaggc taaaaagtgg cctttacgaa agtgtgcgcg
     2401 ccggcgttca gctggctcac atacttgatc acgaaacgat tcgctaagga gtaaacatta
     2461 tgttcagctt cctatggtgg ttgattgtag gcttgatcgt tggagcatta gcgcgattga
     2521 tcatgccggg tagagatgcg atgggaaccc tcgccacaat gctactgggt attgctggat
     2581 caattctcgg cggccttgtc agctgggcca tctggggcac cgataacggc cgatttcaac
     2641 cagccggatt cattctgtca ctgatcggtg caatccttct tctggggctg taccgaatgg
     2701 cgaagagccg gagtgcagtt tagcagttga agtaatagta atttcaaagc ggtgcctgaa
     2761 ctgcccgaca ttgtcgttta catcgaggct ctcgaaaaac gaattctcgg tgaaacactt
     2821 gaacgggtgc aggtcggatc accctttctg ttgcgtacgg ctgtaccacc tttagcaacc
     2881 gcagaaaata gagaggtggt gtcgctacgc agattaggaa agcgaatttg tatcgggctc
     2941 gaaggcgatc tgtggttggt tctacacctc atgatcgccg gacgcctcca ctggtttcca
     3001 aaaggaacca gagcggcgaa gccacgtagt caagcctctc tacagttctc gagcggaata
     3061 cttatactca cagaagccgg tagccagaaa agagcttcgc tccacttgat ccagggagag
     3121 gccggtttaa gacaattgga tccgggcggc ctggaagttc tcgacgcaac cctggaccag
     3181 ttctccaatc ggttactcgc ccgtaatcac actctcaaac gcgcacttac cgatccgaga
     3241 atcttcagtg gtatcggaaa cgcctactct gacgagatac tcttcgaagc tcagctttcg
     3301 cccattctcc aaactcaaaa aatgactcct gaggaaatca aaacactcta cgatgcgaca
     3361 agaaagagtc tgaatgagtg ggtagagcga ctgcgagctg agagccgcga caagtttccg
     3421 gcaaaggtca ctgcgtttcg agcagacatg gccacacatg gaagatttgg caagccttgc
     3481 cctcgctgcg gaggcaagat tcagcgaatt cgctatgcag cgaatgaaac taactattgt
     3541 ccgacttgcc aaacaggcgg gaagttatta gcggatcgtg cgctttcgcg attgctgaaa
     3601 gcagattggc cgaaaaccca ggaagaattt gaaagcctca ctgcggcctc ttagtgttaa
     3661 tcgcccgcga tagcaacaac cctgcccttc gtcgctttca cataatcagc cggcgccagg
     3721 agaatttgca ccccacgaat gcccgcagaa atcgaaatga gatcaaacaa ttcaattgtc
     3781 tcgtcgatgt agactggata atcctttttc cccgcgagcg cagtaacgcc gccgcgaata
     3841 taacccgtca acgttaacaa ttctttcatt ggaaccagat gaatcttgcg atcgcccgaa
     3901 ctcgcagcca aagcttttaa atcgagttca gcgttcgccg gaataacggc aaagcagagt
     3961 cccgcgcgat cgccgcgagc gagtagagtt ttaaaaactt gttccgccgg taagcctatc
     4021 ttcgcagcaa cagtctctgc tcttaaatcg tccggatcaa catcgtattc gcggaggtcg
     4081 tactggattt taagctggtc caaaagtcga gcagcatttg ttttatgcat taacgctgaa
     4141 attcactttg gccgctgatc gtcccaatca ccacatcacg aagctgagga tctggccaaa
     4201 ggagtctccg tcttcaggtg tttactattt gaacttccag aatggtagag ctcgtcgctt
     4261 gggacgttga acactctatt cagtcgagcc cgatagttct caaaagcaag ctgcgcactt
     4321 agttcgagta attgttcttc tgaaaattca ctctgcaaac gagcatacag atcatccgag
     4381 atgtttgcgg gcgccgccac cattgcgtca gcaagttcaa tgactaatcg ttccttctga
     4441 ttgaagaccg aaaaatcgtc gccctcaagc gcaagtaact tttcatcact caacccagct
     4501 cttctgccaa cggcagaatt gatatcaact cagaatgggc aacctatttt gtgagcagta
     4561 cgtaccttgc caagttgaat gtagcgaagc gaaactcgtc gcttcttgct gagacctgct
     4621 tccatcccga tcgtgcccca aacgattccg ggcacgcgca tttgcaat
//
LOCUS       DASSFP010000148         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_930341, whole genome shotgun sequence.
ACCESSION   DASSFP010000148 DASSFP010000000
VERSION     DASSFP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930341"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VFH91_03210"
     CDS             complement(<1..1279)
                     /locus_tag="VFH91_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET6862020.1"
                     /translation="MKNLPRTLRQILCVSLVLYSGTAFGQEVLRWGADPSGGAPYVFT
                     DPSAPDNYIGYEKEMVDALAAAMGRKAEFVPSDWETLVSALQRHSFDIIVNGLEPTGD
                     RAQQILFSKPYYIFQLQLTVRQDDNRIKSLADCKNRTVATLGNTAASRLLEQEHIPFR
                     TYQDPVGAYRDLELKRIDAVLMDVPAEAFYAHGNAKLKPAGPPFTKGLYVIGLRKGDD
                     ALKARVDGAIDKIIVDGTLERILKKWNLWNDAQVEFQAPIRAGTESQPAQFDMTNSSF
                     NWREAMLRLTRAALVTVLIALGSMVIAVVLGLPLALGQWKGPVWLRALCTGYVEFFRG
                     TPVLVQLLFLYFGLPVIGIAMPGWLTALVGLGLNYAAYESQVYRASLEAIPKGQWEAA
                     YLLGMTPLLAFRRIIFPQAFRFALPPMTNDFVSL"
     gene            1504..2463
                     /locus_tag="VFH91_03215"
     CDS             1504..2463
                     /locus_tag="VFH91_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET6862021.1"
                     /translation="MSSTIPVKHNSHRKAPFKEMVWIPGGTFTMGSDHYYPEESPPHS
                     VTVDGFWMDPFTVTNAQFSRFVEETNYVTLAERVPNIADYPGAKPELLVPASVVFRRP
                     GFKVDLRNPYEWWTYIPGANWRRPLGPDSSLKGLAKHPVVHVAYEDVEAYAKWIGKEL
                     PTEAEWEFAARGGLEGADFVWGDEFEPDGAVLANTWQGDFPNENLLKDGYEWTAPVGS
                     FPSNGYGLYEMAGNVWEWTTDWYQQHKDIPQSCCASFNPRGGEKEKSVDPRTPNVVIP
                     RKVMKGGSYLCAPNYCQRYRPAARMAQPIDTSTCHLGFRLVVR"
     gene            2586..3005
                     /locus_tag="VFH91_03220"
     CDS             2586..3005
                     /locus_tag="VFH91_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HET6862022.1"
                     /translation="MLIEFIDEAIKGSLQSPADVGIEQTLGNFVLWIKLFVEMIGALL
                     IAVGAGLAMSRLIKVFLKPTLGGYEGTRLILSRFLSLALEFQLAADILATTVAPSWTQ
                     IGKLGAIAVIRTGLNYFLAGEMKEEEDAIDGTERVTT"
     gene            3029..4066
                     /locus_tag="VFH91_03225"
     CDS             3029..4066
                     /locus_tag="VFH91_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6862023.1"
                     /translation="MHQDVTQSLTDNARTQRICLVILTVIVVGFALYFMRPVLLPLLF
                     ALFLYLCLTPLVNLQHERLRFPYTLSLASTALIGTLVLLLVIVIAASSVAQMAADLNT
                     YQSQLNLLVERISSSIPSEKLGGYEAKNYLQMSEGTISGFISGAVFEFSSLVSNSVFV
                     VILTVFILVGRSQVRSEGLIADLESQVRRYISGTITLSLLTGSLVALTLLLLGVRYAI
                     VFGLFAFLLNFIPLIGPVAATLLPLPVVLLSPELSITAKVLAVVIPAGIEFFMGQIVS
                     PRIIGRSLALHPVTVLLFLLFFQMIWGVGGAFMSTPIAAVTKILFEHFKATQPIAELL
                     AGNLNAILDRE"
     gene            complement(4243..>4536)
                     /locus_tag="VFH91_03230"
     CDS             complement(4243..>4536)
                     /locus_tag="VFH91_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET6862024.1"
                     /translation="EGPAFTTAGAHFNPDSKQHGTKNPMGPHAGDMNNFMVKPDGTAN
                     STVTDPRVNLGADNHSVYSNGGTSIVIHAKADDMKTDPSGNSGDRIACGVISK"
BASE COUNT         1075 a         1126 c         1185 g         1150 t
ORIGIN      
        1 acaaagaaac gaagtcgttt gtcattggtg gtaaggcgaa tcgaaacgcc tgcggaaaga
       61 tgatgcgacg aaaagccagc agtggtgtca tgcccaaaag ataggcagct tcccactgac
      121 ccttcgggat agcttcgagt gaggctcgat agacctgcga ttcgtaggcg gcataattta
      181 gtccgagtcc gaccaacgcc gtaagccatc cgggcatggc gatgccaata actggaaggc
      241 cgaagtataa aaataaaagt tggaccagga ccggagtgcc gcggaagaac tcgacataac
      301 cagtgcacaa agcgcgcagc caaaccggcc ctttccactg gccaagcgca agcggcaaac
      361 ctaagacgac ggcaataacc atcgacccca gtgcaatcaa aacggtaacc agcgcagcac
      421 gtgtgagtcg cagcatagcc tcgcgccaat tgaacgaact attcgtcata tcaaattgag
      481 ctggttgact ctcagtgccg gcgcggatcg gagcttgaaa ttcgacctgg gcatcgttcc
      541 agagattcca cttcttgaga attctttcga gcgttccatc aacgatgatc ttgtcgatcg
      601 ccccgtcgac ccgggctttc agagcgtcat cgcccttccg cagtccgatg acgtaaagtc
      661 cttttgtgaa aggtggacca gcaggtttta acttagcgtt gccatgagcg tagaaggctt
      721 cggccggaac atccatgagc acggcatcga tacgtttcaa ttcgagatcc cggtaggcgc
      781 ccactggatc ctggtaagtg cgaaacggaa tatgctcttg ctcgagcaag cgtgaagccg
      841 ccgtattacc aagcgttgcc actgtcctgt tcttgcaatc ggcgagagat ttaatgcggt
      901 tatcgtcttg tctaaccgtc aattggagtt gaaagatata gtaaggctta ctgaacagta
      961 tctgctgcgc gcggtccccg gtaggttcta atccattgac gatgatatcg aaggagtgtc
     1021 gctgaagagc agagacgaga gtctcccaat cgcttgggac aaactctgct ttgcgaccca
     1081 tcgccgcggc gagtgcgtcg accatctctt tttcgtaacc aatgtagttg tccggggcgg
     1141 aaggatcagt aaagacgtaa ggtgcgccac cggatggatc agcgccccaa cgcagaactt
     1201 cctgtccaaa agcagttcct gagtacaaaa ccagagagac gcacaggatc tggcgcaagg
     1261 ttcgaggaag atttttcaac aatggtgcct ctcataatta ctgccggctg tttcgattga
     1321 gcagaacgga taatagtgat ctctgtcggg actgacaata acgcggccgg ggttagactt
     1381 gggggttgaa ctagtagttc tgacagttat cagaatggcc tgttttgagt acaattcccg
     1441 caaaacgtat cctgcagcga accgattttc aaaaaagcat ctctctgacc caaggtgttg
     1501 ctaatgagca gtacaatccc tgtcaaacac aacagccacc gaaaagctcc atttaaagag
     1561 atggtttgga tccctggtgg aaccttcacg atggggtccg atcactatta ccctgaagaa
     1621 tcgcctcctc attctgttac ggtagatggg ttctggatgg atccgttcac tgtgacgaac
     1681 gctcagttct cacgtttcgt ggaagagaca aactatgtga cgctggcgga acgcgtccca
     1741 aacatcgctg actacccggg agcaaaacca gaattgctgg tgcctgcctc agtagttttc
     1801 agaaggcctg gtttcaaagt agatctcaga aatccatatg aatggtggac ttacattcca
     1861 ggagccaact ggcgcaggcc tcttggtcca gacagctcgt taaaaggttt ggcaaagcat
     1921 cctgtggtcc acgtcgctta tgaagatgtt gaagcctacg ccaagtggat aggaaaagag
     1981 ttgccaactg aagccgaatg ggagtttgcg gctcgaggtg gtttggaagg tgctgatttt
     2041 gtctggggag atgaatttga gccagatgga gcagtgctgg caaacacctg gcaaggagat
     2101 ttccccaacg agaacctgct gaaagacggt tatgagtgga ccgcgcctgt cggatcgttt
     2161 ccatcgaatg gctacggttt atatgagatg gctggcaacg tgtgggagtg gaccacagac
     2221 tggtaccagc aacataagga cattccgcag agttgttgcg catcatttaa cccgcgtgga
     2281 ggtgaaaagg agaagagtgt tgatcctcgc acccctaacg tggtgattcc cagaaaggta
     2341 atgaagggtg gttcatatct ttgcgcgcct aattattgtc agcgatatcg accagcagcg
     2401 cgcatggccc aaccgataga tacgtcaacc tgtcatttgg gattcaggtt ggtggtcaga
     2461 taggaggtca ggggcaggga ctggggatcg gtctttgatc ttgacgttct cgagtgtttg
     2521 tgttttctct ccaacccaag gaccaagacc aagcccaaag gccgacgtag gccacggagt
     2581 ctctgatgct gatcgaattc attgacgaag ctataaaggg ctcactccaa tcgccggcgg
     2641 acgtcggcat tgagcaaacc ctcggaaact ttgttttgtg gattaagctg ttcgtagaga
     2701 tgattggcgc attattgatt gcggttggag ccggtctggc tatgtccaga ctcatcaaag
     2761 tatttcttaa accaactctc ggaggctacg aaggtactcg tttaatcctc tctcgatttt
     2821 tgtcgctggc cctggaattt caactcgctg ccgatatttt agctacaaca gtggccccaa
     2881 gctggaccca aattggtaaa ctaggcgcga ttgccgtaat acggaccggt cttaattatt
     2941 ttctcgctgg ggaaatgaag gaagaagaag acgcaatcga cggtaccgaa cgggtcacaa
     3001 cttaaattct cgcctgggcc aacaaagcat gcatcaagac gtgacgcagt ccttaacgga
     3061 taatgcacga actcaacgaa tctgcctggt cattttgaca gtaatagttg tcggctttgc
     3121 cctctacttt atgaggcctg tgttgctgcc attactgttc gcgcttttcc tttacctctg
     3181 tttgacgcca ctcgtgaacc ttcaacacga gagattgcgg tttccatata ctctttccct
     3241 ggccagcaca gctttgatcg ggaccctcgt tttgttgctc gtgatcgtga ttgcggcttc
     3301 ttcagtggcc cagatggccg cggatctaaa tacctaccaa agccagttga acctgttggt
     3361 ggagagaatt tccagctcta ttccgtcaga gaagctcggt ggctacgaag ccaaaaatta
     3421 tcttcagatg tccgaaggga ctatcagcgg cttcatctcg ggagccgtct tcgagttttc
     3481 gagtttggtc tccaacagtg tgttcgtggt gattctcact gtcttcatac ttgtaggacg
     3541 ttctcaggtc cgcagcgaag ggttgatcgc cgaccttgag agccaggtgc gtcgttacat
     3601 tagcgggacg attacacttt cgttgctgac gggttcactg gttgctctga ccctgcttct
     3661 gctgggcgtg cgatacgcta tcgtgtttgg tctgtttgct tttctgttga attttattcc
     3721 attgattggc cctgtcgccg ctactctgtt gccgctgcct gttgtcctgt tgagtcctga
     3781 gctttcgatt acagccaaag tactcgccgt tgttattcct gctggcatcg aattcttcat
     3841 gggtcaaata gttagcccaa ggatcattgg cagatcgttg gctcttcacc cggtcacagt
     3901 gctgctcttt ttgctcttct ttcagatgat ctggggagtt ggtggcgcat tcatgtctac
     3961 accaatcgca gcagtcacta agattctttt tgagcacttc aaagccacac aaccaatcgc
     4021 cgaacttctc gccggcaacc tgaatgcaat tctggatcgc gagtaattca gagcactcct
     4081 ccggagtgct ctgaattccg gattttatag gcctcacatt gctgtcgaac aggtagttag
     4141 tggtgactct ttttcttccg aacacaacaa acgtgttacc catgtctccg gactataccg
     4201 catggaccac tgacaacgga ccactgacca ctgacaactg gcttacttac taatgacacc
     4261 acaagcgatc cggtctccgg agttgccgga cgggtcggtc ttcatatcgt ctgccttagc
     4321 gtgaatgacg atggatgttc cgccattgct gtaaacggaa tgattatcgg cgcccaggtt
     4381 gacccgcgga tcagtcaccg ttgagtttgc tgttccgtct ggcttaacca tgaagttatt
     4441 catgtcgcca gcatgtggtc ccatcgggtt cttggtcccg tgctgttttg aatcgggatt
     4501 gaaatgcgcg cccgcggttg taaacgctgg accttc
//
LOCUS       DASSFP010000149         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1674145, whole genome shotgun sequence.
ACCESSION   DASSFP010000149 DASSFP010000000
VERSION     DASSFP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674145"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /gene="alr"
                     /locus_tag="VFH91_03235"
     CDS             complement(<1..651)
                     /gene="alr"
                     /locus_tag="VFH91_03235"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET6862025.1"
                     /translation="MASDSLNTRPAPGSRTTWAEINLDNLSHNYRVVQQRVGPEVKVM
                     AMLKADAYGHGAVRCAQSLSAAGVQWLGVALPEEALELRDAGIRVPILCVGGFWSGQE
                     QLCLNNNITPVVYRLDMIESLNRAAAAANVVADVHIKIDTGMGRLGIRWDHLNDFVLQ
                     LSRFKNVRIDGLMTHLAAADDASCQPLTRDQIGRFEESVSLFRQHGYQPTHFHLANS"
     gene            complement(791..2167)
                     /gene="dnaB"
                     /locus_tag="VFH91_03240"
     CDS             complement(791..2167)
                     /gene="dnaB"
                     /locus_tag="VFH91_03240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET6862026.1"
                     /translation="MASADPTRDQQFERALPNSPEAERAILGAILLDNGLVSQAIEQL
                     RPEDFYIPAHRRIFVAMITLFERGAEINPILIGEELRKENSLESVGGFSSINNLTYGL
                     PHSTNIIHYAQVVRGKSMLRQLIQVSNKIIQEAVEEEDEPALILDHAEHAIFQLADER
                     IRQGFTHVKPIAEQLLEKVQEMEGRAAVLTGLTTGFSDLDKMTSGLQRSDLIILAARP
                     SMGKTSFALMLAENAALHSGAVVGVFSLEMSKESLVMRMLCSQGNIDAQRFRNGFLSR
                     LEWAQIAKSLGVLADAKIYLDDTPGLSVLEMRAKARRLAAEQKKLDLIIVDYLQLMSG
                     SSKRAESRQQEVSQISRELKALAKEMNVPLVALSQLSRAPEARSDHRPQLADLRESGA
                     LEQDADVVAFIFREEQYKTPEERDAMPEDQKNIAEIIIAKQRNGPTGTVDLRFTPSSM
                     RFDNLYRE"
     gene            complement(2293..2835)
                     /gene="rplI"
                     /locus_tag="VFH91_03245"
     CDS             complement(2293..2835)
                     /gene="rplI"
                     /locus_tag="VFH91_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET6862027.1"
                     /translation="MAHTKVLLREDVDDLGARGEIVRVRAGYARNYLLPRNLAVQATA
                     GNVKGIEAERAALLKKEAKERSTAEGQSKQIGSLVLEFKRKAGEQGALYGSVTSMDLA
                     EALKERGYEIDRHRIHLREPLKRVGDYSVPIRLHREVTIDLQVKVVPEGEVIVGHLTP
                     EQEAELAKQNAADDSANPSA"
     gene            complement(2850..3059)
                     /gene="rpsR"
                     /locus_tag="VFH91_03250"
     CDS             complement(2850..3059)
                     /gene="rpsR"
                     /locus_tag="VFH91_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET6862028.1"
                     /translation="MHRRRVCRFCIDKTDLIDFKDVRLLQNYLPERGKILPRRISGSC
                     ATHQRMLAEAIKRARNIALLPYTTD"
     gene            complement(3184..3600)
                     /gene="rpsF"
                     /locus_tag="VFH91_03255"
     CDS             complement(3184..3600)
                     /gene="rpsF"
                     /locus_tag="VFH91_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET6862029.1"
                     /translation="MDTNRQYEVVFIVDPTADDEVGRLTDSLKQVVTDQGGTVTKAET
                     MGKRQLAYEIDHKKEGTFVLLEIEGSGREIAELERRMRVNDRVLRYLTVRVDEDRRRA
                     DKLKDRRARKAAKRPVPGSSGRRGSAMEFSDAGGGE"
     gene            complement(3628..>4101)
                     /gene="pth"
                     /locus_tag="VFH91_03260"
     CDS             complement(3628..>4101)
                     /gene="pth"
                     /locus_tag="VFH91_03260"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET6862030.1"
                     /translation="ASDAGVTVGRRECSSLVGRGELEGSVVKLVKPQTFMNLSGQAVS
                     CLLAKEQERPLDHLLVISDDLALPVGRIRIRERGTAGGHNGLKSIISSLGTNEFMRLR
                     IGIQPEHPISDTRRFVLDSFAKSERALVEESIALSAKAVRTIIGEGVLKAMSEFN"
BASE COUNT          962 a         1132 c          976 g         1031 t
ORIGIN      
        1 agagtttgcc agatgaaagt gggtcggttg gtaaccgtgt tggcgaaaca gactgacaga
       61 ttcttcaaag cgtccaatct gatcacgagt taagggttga cagctagcgt cgtcagcggc
      121 cgccaagtgc gtcatcaaac catcgatgcg gacgttctta aaacggctta actgcaaaac
      181 aaaatcgttc aaatgatccc aacgaatgcc aagccgcccc atccctgtgt cgattttaat
      241 atgaacgtca gcaactacat tggctgcagc ggcagcgcga tttaaagact cgatcatgtc
      301 gagccggtaa acaacaggag tgatgttgtt attcaggcaa agttgttcct ggccgctcca
      361 gaatccgccg acacataaga ttgggacccg gatccccgcg tcgcgtagtt ccaatgcttc
      421 ttcgggtaac gcaactccta accattgaac acctgcagcg gaaagactct gggcgcagcg
      481 aacagctccg tgaccatagg catcggcctt caacattgcc atcactttta cttctggacc
      541 aacgcgttgt tggaccacgc ggtaattgtg cgagaggttg tcgagattga tttctgccca
      601 ggttgtgcgt gaacctggag ccggtcgtgt gttaagtgag tcagatgcca tgtcttgcca
      661 gtcttctcca gcaaaagatt atccgcagat tacgccgatt acacagattt ttcagctcaa
      721 atggtgattt ctaattcgtc agcgttttga gaatcggcgt aatcagcgca atctgtggct
      781 gatcctcaaa ctattctcga tacagattat cgaagcgcat gctggagggt gtgaagcgca
      841 aatctactgt tccggtgggg ccattgcgct gcttggcaat aattatttcc gcaatattct
      901 tctgatcttc cggcatcgca tctcgctctt ccggagtctt gtactgctct tcgcgaaaga
      961 tgaacgcaac aacgtctgca tcctgctcga gcgctcctga ttcacgcaga tcagctaact
     1021 gaggacgatg atccgaacgc gcctcgggtg ctcgtgacaa ctgagataac gcaaccagag
     1081 ggacattcat ctccttagcc aacgccttca gctctcgcga gatttgcgat acttcctgtt
     1141 gacgtgattc ggctctcttt gacgaaccac tcatgagctg caagtagtcg actattatca
     1201 agtcgagctt cttttgttcg gcggcgagac ggcgagcctt ggcacgcatc tcgagcacac
     1261 tcaaaccggg cgtatcgtcg aggtaaatct tagcatcagc cagcacacct aacgacttag
     1321 cgatttgcgc ccactccaaa cgggacagaa atccattccg aaagcgctga gcatcgatgt
     1381 ttccctgtga gcacaacatg cgcatgacga gtgattcttt cgacatttcc agcgagaaca
     1441 ccccaaccac cgcgccactg tgaagagccg cattttcagc taacatcaac gcaaaactgg
     1501 tcttacccat cgacggtcgc gcggccagaa ttatcaaatc tgagcgctgc aggcctgaag
     1561 tcatcttgtc gagatcggag aagccggttg tgaggccggt caatacagcc gcgcgacctt
     1621 ccatctcctg gaccttttcc agcagctgtt cggcaatcgg tttgacgtga gtaaagccct
     1681 ggcgaattcg ttcatccgca agttggaaga tcgcgtgctc cgcatgatcc aggatcaacg
     1741 cgggctcgtc ctcttcctcc actgcttcct gaataatctt gttcgagact tgtatgagct
     1801 gtcgcagcat cgacttgccg cgtacaactt gagcgtaatg aatgatgttc gtggagtgtg
     1861 gaagaccgta tgtcagattg ttgatggaag agaatccgcc aacagactcg agagagtttt
     1921 cttttcttaa ctcctcaccg attaggattg gattgatttc ggcgccacgc tcgaagagag
     1981 taatcattgc gacgaagatg cgccgatgag ccggaatgta aaaatcttcc ggtcgcaatt
     2041 gctctatggc ctggctgacg agtccgttgt cgagaagaat agcgccgagt atagctctct
     2101 cagcttccgg gctattgggg agggcacgtt cgaactgttg gtcgcgagtg ggatcagcgc
     2161 tggccatttg aatagagggg ttgtcgctaa cgtgatgatg ttacttagaa aaggcgttgc
     2221 aggctcattg cccacaacgc ccagatttta cacgaaaaga cggcgcctca ttcatgacag
     2281 ctatgtttta agttacgccg aagggttagc gctatcgtcg gctgcatttt gtttcgccag
     2341 ctctgcttcc tgctctgggg tgagatggcc gacgataact tcaccttccg gcacaacctt
     2401 cacttgcaga tcgatagtga cttcgcgatg cagtctgatg ggaactgaat aatcgccaac
     2461 acgcttcaat ggttcgcgca agtgaatgcg atgacgatca atttcgtagc cacgctcttt
     2521 gagtgcttcg gccaaatcca ttgaggtcac tgaaccataa agtgcgcctt gttctcccgc
     2581 ttttcgtttg aattcaagca ccagcgaacc aatctgtttg gactggcctt cagcggtgga
     2641 tcgttctttg gcctcttttt tgagcagagc agcgcgttca gcttcaatac ccttcacgtt
     2701 accggccgtg gcttgaacgg cgaggttacg gggtaacagg taatttcgag cataccccgc
     2761 ccgtactctg acgatctcgc cacgtgctcc cagatcatca acatcctctc gtaggagtac
     2821 tttggtgtgt gccatttaca gttcctctct taatccgttg tgtatggaag gagcgcgata
     2881 tttcgagcgc gcttgatcgc ttccgccaac attctctggt gagtcgcaca gctccctgaa
     2941 attctgcgag gcaaaatctt gccacgttcc ggcagatagt tctgcaacaa cctcacatct
     3001 ttaaaatcga tgagatcagt cttgtcgatg caaaatcgac aaactctgcg acgatgcatt
     3061 cggcggccac cgccgcgacc catcgaccgt ctatcctgat cgtcgtcact gtcataaccg
     3121 ccgccacgcg ggcgatcttc atagttgctc gacataatta tcctcttact tattgatcaa
     3181 aaattattcg ccgccaccag cgtcggaaaa ttccatcgct gatccacgcc tgcccgatga
     3241 accgggcacg ggacgcttgg cagctttccg tgcacgcctg tctttcaact tatcggcccg
     3301 gcgtctgtcc tcatcaactc gcacggtcaa ataacgaagc acccgatcat tgacgcgcat
     3361 acgtcgctcg agctcggcaa tttcgcgtcc ggatccttcg atctccaaca acacgaatgt
     3421 gccttccttc ttatggtcaa tctcatacgc aagctggcgc ttgcccatcg tttcagcctt
     3481 cgtgacagtg ccaccctggt ctgtaactac ttgttttaag ctgtccgtca ggcggccaac
     3541 ttcatcatcg gccgttggat cgacgatgaa aaccacttcg tactgtctat ttgtatccaa
     3601 agcaacctcc agttatggac gaggaattca attgaactca ctcatcgctt tcaaaacacc
     3661 ttcaccaatt attgttcgca cagccttcgc actaagagca attgattctt ccactaacgc
     3721 tctctcgctt ttagcaaatg agtcaagcac aaaccgtctg gtatcggata tcgggtgttc
     3781 aggctgaatt ccgatacgca atctcatgaa ctcgtttgtt cccaaagaac taattatcga
     3841 cttcaaaccg ttgtgaccac ctgcggtgcc acgctcgcga attcgaattc tcccaaccgg
     3901 caaagccaga tcatcagaaa tcacaagcaa gtggtccaat ggtcgttcct gttctttcgc
     3961 gagcaaacag gagaccgctt gtccactaag attcatgaag gtctgcggct tcacaagttt
     4021 gaccacgcta ccttccagct cgccacgacc gacaagcgaa ctacactcgc gacgtccaac
     4081 ggtcacccct gcatccgagg c
//
LOCUS       DASSFP010000150         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_335426, whole genome shotgun sequence.
ACCESSION   DASSFP010000150 DASSFP010000000
VERSION     DASSFP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335426"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..663
                     /locus_tag="VFH91_03265"
     CDS             178..663
                     /locus_tag="VFH91_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HET6862031.1"
                     /translation="MRLSLRSFSTAIITIAALMVLTAMVVVSQGPQGQGGPPPGGEFG
                     FGPGPRGGFPFLRGLNLTDDQKAQIGKITESLEASTKDLRDQMRALHANETNPLTTPF
                     DEASVRAAAEARAKIEVELQVAHAKAMSQIGAILTTDQKTQLAARRPHRPEGPPPSPS
                     N"
     gene            962..>3220
                     /locus_tag="VFH91_03270"
     CDS             962..>3220
                     /locus_tag="VFH91_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HET6862032.1"
                     /translation="MKTIISIAAFSILLLHSIFQQFDYSQTKAEAERVYAAGSYARSN
                     ELYARIDKTKLSLQDRRWVEFRLADTSWRSQSATQTSDSTLIDTAQKQLDELIRVNDK
                     PEDRDRVWAEAHESLGDLFWTRQNQMNWGAAWPHYQQALDWWAGQRSIDEARTRYLKI
                     VFRAAEPPKVAEYYYYGYYGNYIPITTLEDALKISIGESDKSHLHFLIAMSLRYTGGD
                     WESRQRVPDEFEEALKAGKQSPWYDDALYSYAEWMNTTGAIVQVKEGEWQQRPDFVKA
                     LELYRRLVAEFSKGESRYYDNAQQQINNITLPNLSVAVANVFLPQSEIQFVISARNVP
                     SVTLSLFRVDLSPEPMLAGDNPDASLSLWLQRISLGDKRPVKSWTEKIDSTAYKLTSN
                     QIRITEKLPIGSYVLLANGSGQTARELILVSDASLVLMSGSKQASIYFANAITGAPIA
                     NANVVLWDGYQVNNRWTWKKLVQTTDQDGAAHFGLTPETAYHVLLAIADSDARQAFAM
                     SSSYYGSGLADWRIYAFTDRPAYRPKETMQFKFIARRYSQGQYTTPANQTIEYEINDP
                     KGTKVSDGKATLNSFGSSWGSLELTEQLPLGEYNIQFWEAGRVRSIGSAHLFRLEEYK
                     LPEFKVAVKTPQENGKRKAFRLGEQVEVNIQADYYFGGPVSNASVDVVVYQKPYFQYW
                     PRTREYPWYYDDLDVQRRGYYGNGQVIKREAIKTDATGKAVLSFETPRENYNQDFEYR
                     IEARVTDSSRREI"
BASE COUNT          839 a          795 c          828 g          758 t
ORIGIN      
        1 agtttcagct tcagccgtaa aagaacggag aaaggcgaag cgcgtttcgt cagttttacc
       61 agcgcgggtg gaactggtta gcgcttgccg tatgggttct ttgttttttc gactcggaat
      121 agcgagcgcg gctgaagctg aaagtgatta tggttgcagg aattgaagga gaaaaagatg
      181 agacttagtt tgagaagttt ttctaccgca ataattacca tagcagcact gatggtgctg
      241 actgcgatgg tcgtcgtctc tcagggtcca cagggacagg gcgggccgcc gccgggtggt
      301 gagttcggtt ttggccctgg accgcgtggc ggattcccat tcttacgtgg cctaaatttg
      361 actgacgatc aaaaagcaca gatcggcaag atcactgagt cgttagaagc cagcaccaaa
      421 gacctccgtg atcagatgcg agcattgcat gctaatgaaa caaacccatt gaccactccc
      481 tttgacgaag cctctgtacg agctgctgct gaagcacgag ccaaaatcga ggttgagttg
      541 caggtagcac acgcaaaggc aatgtctcag attggagcaa tactgaccac tgatcaaaag
      601 actcagttgg cggctcggcg tcctcataga cccgaaggac cgccgccttc accatccaac
      661 taattttgct caagcttgag cactccccgg atggcaagac tgggtaggcg atgaggaccc
      721 tgaatcaaat accggacctc ttcagggtct tcgagggagt tcgcctgccc atattgccgt
      781 ataagaccat ctgccctatt ttttccctgc aagccaccgc ctctctgatc gttcatatcc
      841 cgggcgatca actccgctat aatcgggctg tcacctcgtc ggactgttct gtcgccgaac
      901 ttctcggttc ccgagttaaa tagcaatcta atgtttagac ctcaattcag gcaatctcat
      961 catgaaaacc atcatctcaa tcgccgcgtt ttccatcctc ttgcttcatt cgatctttca
     1021 gcaattcgat tacagccaaa ctaaagcaga ggccgaacga gtttacgccg ccgggtcgta
     1081 cgcgcggtcg aatgagttgt acgccagaat cgacaaaaca aaattgagcc tgcaagaccg
     1141 caggtgggtt gagtttcgac ttgccgacac ctcgtggcga tctcaatcag caacgcaaac
     1201 ttccgactca acactcatcg atacagctca gaagcagctg gacgagctca tacgagtcaa
     1261 cgacaaaccg gaggatcgag accgagtttg ggccgaagcg catgagtctc ttggcgatct
     1321 cttttggacc agacagaacc agatgaactg gggcgctgcg tggccacatt accaacaagc
     1381 acttgactgg tgggccggcc aacgatcgat cgatgaagca cgcactcgtt acttgaaaat
     1441 cgtttttaga gcggccgagc ctccaaaagt tgcagagtat tactactacg gctactacgg
     1501 aaattatatt ccgatcacca ctctggaaga tgccctcaaa atcagtatcg gagaatccga
     1561 caagtcacat ctgcactttc ttattgcgat gtcactgcga tatacgggcg gagactggga
     1621 gagccgtcaa cgagtccctg atgaatttga agaggctcta aaggcaggca aacaatcacc
     1681 ctggtacgat gatgctctct actcgtatgc agagtggatg aatacaactg gagctattgt
     1741 tcaggttaag gagggggagt ggcagcaacg gcccgatttt gttaaggcac ttgaacttta
     1801 tcggcgactg gtagctgagt ttagtaaggg cgaaagtcgt tactacgata acgcgcaaca
     1861 gcaaataaac aatattaccc ttccaaactt atcggtcgcg gttgccaatg ttttccttcc
     1921 tcaatcggag attcagttcg tcatcagtgc tcgcaatgtt ccctcggtga ctctctcact
     1981 attcagagtc gacctaagtc ctgaaccaat gttagcgggt gacaatcctg atgcctcact
     2041 gagcctgtgg ctccagagaa tctcgcttgg tgacaagcgt ccggtaaagt catggacgga
     2101 aaaaattgac tccacggcct acaaactaac tagcaatcaa atccgaatca ccgagaaatt
     2161 gccgattggt tcctatgtcc tgcttgctaa tggctcgggt cagacggcgc gcgaattgat
     2221 tcttgtcagc gacgcttcat tggttttgat gtcagggtcg aagcaagcgt caatctattt
     2281 tgcgaatgcc atcactggag cacccattgc taacgcaaat gtcgttcttt gggatgggta
     2341 ccaggtgaat aaccggtgga cgtggaagaa gttagtccaa acgactgacc aggatggcgc
     2401 ggctcatttt ggattgacgc cggagactgc gtaccacgtt ctattggcga tcgcggatag
     2461 tgatgcacgt caggcgtttg cgatgagttc atcgtattac ggttcgggcc tggctgattg
     2521 gcgcatctat gcgtttaccg atcgacctgc gtaccgacca aaagagacaa tgcaattcaa
     2581 atttattgca cgccgttata gccagggcca gtacacaacc ccagccaatc aaaccattga
     2641 atatgagatc aacgatccta aagggacgaa agtctcggac ggcaaagcta ctttgaactc
     2701 attcggaagt tcatggggtt ctctggaact tacggagcag ttgccgttgg gagaatacaa
     2761 cattcaattt tgggaagcgg gacgcgtaag atcgattggc agtgcgcatc tattcaggtt
     2821 ggaggagtac aagcttccgg aattcaaggt tgcggtgaaa actccacagg aaaacggaaa
     2881 acgcaaagcc ttccgtttag gtgagcaggt cgaggtaaac attcaagccg attactattt
     2941 cggcggacca gtcagcaacg ccagtgtcga cgtcgttgtt tatcagaagc catattttca
     3001 gtactggcca cgaacacgcg aatacccgtg gtactacgac gatctcgatg ttcagcgtcg
     3061 cggatactat ggaaacggtc aggtgataaa acgtgaggcg atcaagaccg atgctaccgg
     3121 gaaggcggtc ttgagcttcg agaccccgcg tgaaaattac aaccaggatt ttgagtatcg
     3181 aatagaagcg cgcgttactg attcatctcg acgcgagatt
//
LOCUS       DASSFP010000151         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_484344, whole genome shotgun sequence.
ACCESSION   DASSFP010000151 DASSFP010000000
VERSION     DASSFP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484344"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..662
                     /locus_tag="VFH91_03275"
     CDS             243..662
                     /locus_tag="VFH91_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6862033.1"
                     /translation="MKSYRKELWFDVPTRRAFINITSDVQACLRDSKIHEGLALVNAM
                     HITASVFINDDESGLHQDYENFLEKLAPHEPTSHYRHNLTGEDNGDAHIKRQLMGREV
                     VVAVTDARLDFGPWEQIFYGEFDGGRKKRVLVKIIGD"
     gene            863..1912
                     /locus_tag="VFH91_03280"
     CDS             863..1912
                     /locus_tag="VFH91_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862034.1"
                     /translation="MEQPAVTIDALLEPLLGASDEHVDEFLPRLIAEHAEPVIKGVVR
                     YKLRLNSSQASQRAEADDIYQEVLVQFLSQVQQFRQKPHQHPITDVRGMAAVIAHRTC
                     SRWMRKQFPERHALKNRLHYLLTRQRGLSVWQDEDGKLVAGFAAWQGKTKQTITSQAE
                     SLSVNKELLGQVRMLKSGKQQELSAALATIFNKVNGPTDFDELVSFVASLIGIRDQPV
                     ESLADDDDSASFVPTSEQPDQAWQVEKRMFLQRLWEELQQLPTNQRAALLLNLRDASG
                     HSCITLFPATGVATLKQLAEVLQMPLEDFAKLWNELPVEDARIAELLGLTRQQVINAR
                     KSGRERLARRLKGFM"
     gene            2018..>2530
                     /locus_tag="VFH91_03285"
     CDS             2018..>2530
                     /locus_tag="VFH91_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862035.1"
                     /translation="MLEHLNKQQIQNYVSQQLELAELLTVSEHLEECEACRHQVQAEM
                     NSDVPFLAMHSTIAEEAPIAPNHLTAEQTADYVDKSLPSDEQQIASDHVSTCELCQLA
                     VEDLRAFRNEIAPSLDIEYYPVTTTAPGPSLWQRFVTQLPVGIRSPAQAFGAALVILV
                     LGITGWLIWRA"
BASE COUNT          681 a          611 c          646 g          592 t
ORIGIN      
        1 ctccgttcga agttctaaga aggagcgact aggactttag gcttcaagat tctaccgctc
       61 cttcttagaa cttcgaacgg agtgggctgt ctctggggag ttgtcggcta taaacatatc
      121 accccacggg gtgaaacccg acgctgacac gaatcggtac ttacggtgtc aacgggttac
      181 gacagtgtta ggattttaaa aaagtttcag aaaacaatga ataacgtgaa ggtggtggag
      241 aaatgaagag ttatcgaaaa gagctttggt ttgacgtgcc aacgcgacga gcatttatca
      301 atatcacatc ggacgtccaa gcttgcctga gagacagtaa aatccatgaa gggctcgcat
      361 tagtcaatgc aatgcatatt accgcttcag ttttcattaa tgatgatgaa agcggtctac
      421 atcaagacta cgagaatttt ctcgagaagc tggcgccgca cgagccaacg agccattaca
      481 ggcacaactt aaccggcgag gataatgggg atgctcatat caaacgacaa ttaatgggcc
      541 gtgaagtagt tgtagccgtt accgatgctc gattggattt cgggccgtgg gaacaaatct
      601 tttatggaga gtttgatggt gggcgaaaga agcgagtgct cgtaaagatt attggcgact
      661 aagatccttc tccttctgtt actagcctgt agatcttccg gttcacccgt caggaaaacg
      721 gtcgcaatga gctagactac cctcgacatc gcgaagcgac ttatcgacta cgggtctttc
      781 ctcattgaat ccctaacggt ttcaatgtta tgagaacctt gcgaacaatc ctggcccagg
      841 tcggatcgta gtccaatact gaatggaaca gccggcggtc acaattgatg cactgctcga
      901 gccgttgctc ggcgcaagcg atgaacacgt cgacgagttt ctgcctcgac tcattgccga
      961 acatgccgaa ccggtaatca aaggcgtcgt tagatacaaa ttgcgcctca actcgtcgca
     1021 ggcaagtcaa cgcgcagaag ccgatgacat ttatcaggag gtgttggtcc agttccttag
     1081 ccaggtgcag caattccgcc aaaaaccaca tcaacatcca atcaccgacg tccggggaat
     1141 ggcagcggtc attgcccatc gcacctgctc gcgctggatg agaaagcagt ttccggagcg
     1201 acacgcttta aaaaaccgtc tccactatct cttaactcgc cagcgcgggt tatcagtctg
     1261 gcaggatgaa gacggaaaat tagtggccgg atttgccgca tggcaaggca agacgaaaca
     1321 gacgattact tctcaagcag aaagtctttc cgtcaacaag gagctgttgg gacaggttcg
     1381 aatgctcaag agtggtaagc aacaggagtt gagcgcagcg ctcgccacca ttttcaataa
     1441 agttaatggt ccaacagact ttgatgagct ggtaagtttc gtcgcctcgt taatcggcat
     1501 tcgggatcag cccgtcgagt cacttgccga tgatgacgac tccgcttcgt ttgttcctac
     1561 ttcagagcaa ccggatcaag cctggcaagt agaaaaacgg atgttcctgc aaagactatg
     1621 ggaagagttg caacagctac caacgaatca gcgtgctgcc ttgctactaa atcttaggga
     1681 cgcgagcggg catagttgca tcacgctttt tccagccact ggagtcgcaa ctttaaaaca
     1741 acttgccgag gtgcttcaga tgcctctgga agattttgca aagttgtgga acgaactgcc
     1801 cgtcgaggat gcgcgaattg ccgaattgct cggattgacg cgccaacagg taataaatgc
     1861 gaggaaatcg ggccgcgaac ggttggcgcg acgactgaaa ggatttatgt gagtcgctat
     1921 agtaataaag gacaattttc gacaacttat cagtttggcg accctttgcg gtatcgctca
     1981 agcacagcca acacccgcgg ctgcaaggat ttaagagttg ttagaacatc ttaacaaaca
     2041 acaaattcag aattacgttt cgcagcagct ggagcttgcg gaactgctga ccgtgtctga
     2101 acatctggag gaatgcgaag cgtgcaggca tcaagttcag gctgagatga atagcgatgt
     2161 tcccttcctc gcaatgcact cgacaatcgc ggaagaggcg cccattgctc caaatcacct
     2221 tacagcggaa cagacagccg attatgttga caagagcctg ccttcagacg aacaacagat
     2281 cgcttctgat catgtctcaa cgtgtgagtt gtgccagttg gctgttgagg atcttcgcgc
     2341 attcagaaac gaaatcgccc cttcactcga tattgagtac tatcccgtca caacaactgc
     2401 gcctggtcca agtctctggc aacgattcgt aacacagtta ccggttggga taaggtctcc
     2461 agcacaagct ttcggcgcag cactcgtgat tctcgtttta ggaataactg gttggctaat
     2521 ctggagggct
//
LOCUS       DASSFP010000152         4904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_775995, whole genome shotgun sequence.
ACCESSION   DASSFP010000152 DASSFP010000000
VERSION     DASSFP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775995"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VFH91_03290"
     CDS             complement(<1..1167)
                     /locus_tag="VFH91_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862036.1"
                     /translation="MINWLRLLLMTFYAPWRGMREVRDRAALAPTAFVALLTQLAYAA
                     VTRKFAGNLSLHSGGAIFGELFQAAITVVLIAVVLVPILTLVANTFDRRGSFRVVITQ
                     EYAPVATSMFYVLMAANLISILIAVLVHYSGVQANQVAIMIQQADQTRALFPIPDTPQ
                     FAPVAQQLAEQLRDPGALSESLFFMPKFILFSIGMIFAVRDSFRISALRAVGVIFVSC
                     FIALFVAPIAVQLFSGVLGSPFLIILLFFLLRGYFSDIVGTQRARAAFKQNLEAATLN
                     PADASAHYNLGLIHQNRGELDAARERFERSVQIDPEEIDAYYQLGRIARQQKRFSDAV
                     QNFEQVVARDPAHSQYEVWREVAATYISANQFDDARNALEQFLEHRPSDPEGLYL"
     gene            complement(1187..1903)
                     /gene="lepB"
                     /locus_tag="VFH91_03295"
     CDS             complement(1187..1903)
                     /gene="lepB"
                     /locus_tag="VFH91_03295"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6862037.1"
                     /translation="MLGIRKRQPPPFGGIHIDDDKDIQIHRPEEVSRNDLWSEARLLL
                     RDLIFALMIAALVVVFIVQPVKVEGTSMLPRLHDGERIFVNKLIYYDEYRWAPKLDRG
                     DIVVFWFPDDPSKSYIKRVIGLPGDTVEVHEGIVRVNGQDLEEGYLDPSLNLSHRSQA
                     PIYVRPNYYFVMGDNRDNSSDSRIWGLVPKKYIYGKALFRYWPLTAASVIHHENIPTT
                     GPPPGFRPDFQDVPANENPQ"
     gene            2073..2510
                     /locus_tag="VFH91_03300"
     CDS             2073..2510
                     /locus_tag="VFH91_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6862038.1"
                     /translation="MVIDVQTTSDGQLTTDKLLDSLCARIYHPDLHLQPRSIRAPAEK
                     GHLTVIKLDIINRVADKTGVPKMKAEQAVEALFQSMKEALTRGERIELRGFGVFVVKP
                     RKRGVGRNPRTGEEVAIPSGKTIRFKPGKELQAHEDGDSEDSI"
     gene            2592..3590
                     /locus_tag="VFH91_03305"
     CDS             2592..3590
                     /locus_tag="VFH91_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6862039.1"
                     /translation="MAETLRTAFSLYPQPGLKPTARDWRLHSSLLFLTAVTTTFAGLM
                     LVSPQLPTPEPALTNPLDYLLYLPLSYYYSVVELLRYSFSHPYLLGQAGAFAASLLAI
                     LMSHEMGHYLACRYYGVDATLPYFIPAPPLFLAGTFGAFIKIRSPIPSRRALFDIGLA
                     GPLAGFIVAVPVAVIGILTIGPPVTQGSGIVFNDPLLFRFVARMFGVHLDPHSTISPY
                     YMAAWIGLLVTSLNLMPVGQLDGGHGTFAVFGEQVHRIVGKVAFVCTGILAILGFWWY
                     SSPSGFLYTILLGIMLRVRHPQPAVMEPLGSKRVLIAILTLIVFVLCFAPFPIAID"
     gene            complement(3595..4560)
                     /locus_tag="VFH91_03310"
     CDS             complement(3595..4560)
                     /locus_tag="VFH91_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862040.1"
                     /translation="MADYKDRIDEWQQATLRKARELNEKFGLSDIVGEGARAAENAAR
                     KGVETVASGAERLRAEAEKLSSDEELGDAARKAANETMRGVKKASETFRDAAGDAGKK
                     AGEVFEGARTYYDRASQFYGTGAKFSRASAAATIGMLKARDWIKENPGKAAVVSVSLV
                     LGIRAGASFPGLDAVLLGSHPHWLTHSALPVYGLRKASEKFDSYLKEQEKLIATGALS
                     EAEQKRAEFERQIVKYVGAPLLGAFSCAAGAAMWAQIFQPGAITGAPISWLLGGNPFL
                     DGVWLFGNGVICFHQGYKFFMIALANQDEVARVVREIKGLLPAGA"
     gene            complement(4563..>4904)
                     /locus_tag="VFH91_03315"
     CDS             complement(4563..>4904)
                     /locus_tag="VFH91_03315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6862041.1"
                     /translation="GIDLAARIGGEEFAVLLVETGLEGGLEVAERLRTAINTLEIPTA
                     SGITASFGVAECPSSGHTAGAILKAADIALYEAKKTGRNRVVSIEPAQPNSGTAGDVH
                     PARDRRVASRG"
BASE COUNT         1159 a         1320 c         1231 g         1194 t
ORIGIN      
        1 cagatataga ccttccggat ctgaaggtcg atgttccagg aattgctcga gagcattacg
       61 agcgtcatca aactggtttg ctgagatata agttgccgcc acttcgcgcc aaacttcgta
      121 ctgcgaatgg gccggatctc gcgctacgac ctgctcgaag ttttgcacgg catctgaaaa
      181 tctcttctgt tgtcgtgcta ttcgccctag ctgatagtag gcgtcgatct cttccggatc
      241 gatctgcacg gagcgttcaa agcgctcacg cgcggcgtcg agttcgccgc ggttttgatg
      301 gataagtccc agattgtaat gggctgatgc atccgctgga ttcagcgtag cggcctcaag
      361 attttgtttg aacgcagctc gcgctcgctg tgtgccaacg atgtcgctga aataaccgcg
      421 cagcagaaag aagagaagta ttatcaaaaa aggtgatcca aggacacccg agaacaattg
      481 caccgcgatt ggcgcgacga agagagctat aaaacacgat acaaagatta cgccgacagc
      541 ccgaagagct gagatacgaa aactatcccg aacggcgaag atcattccaa tcgagaagag
      601 gataaactta ggcatgaaga agagactctc ggaaagagcc cccggatctc gcagctgctc
      661 cgccagttgt tgtgcaacgg gtgcgaattg aggtgtgtcc ggaatcggga acagtgcacg
      721 tgtttgatcc gcttgctgga tcattatcgc gacctgattc gcttgaacgc ctgagtagtg
      781 aactaagact gctatcaaaa tcgaaatgag gttcgcagcc atcaaaacgt aaaacatcga
      841 ggttgcaaca ggagcgtact cctgcgtaat gacaacgcga aagctgccac ggcgatcaaa
      901 ggtatttgcc acgagggtca gaatggggac cagaacgact gcaatgagaa ctactgtaat
      961 tgcggcttga aagagttcac caaaaatagc gccgcctgaa tgcaacgaca agtttcccgc
     1021 aaacttacgt gtcaccgctg cgtaagcaag ttgagtcagt aaggcgacaa aagctgttgg
     1081 agctagcgct gcccgatctc gaacctcacg cattccacgc cagggtgcgt agaacgtcat
     1141 caataaaagg cgcagccagt taatcatact cgaatgggaa atttaactat tggggatttt
     1201 cgtttgccgg cacatcctga aaatcaggac gaaaacctgg gggtggacca gtagtcggaa
     1261 tattttcatg gtgaatcacg ctggccgcgg tcaggggcca gtatctgaac agcgctttcc
     1321 cgtaaatata tttttttggc acgagtcccc agatccgcga gtcactcgag ttgtcgcgat
     1381 tatcacccat tacgaaatag tagttcggcc tcacgtagat gggagcctgg cttcgatgcg
     1441 acaaattcag actcggatcg agatagccct cttccagatc ctgtccgttg acgcgcacga
     1501 tgccctcatg aacttcgact gtatcgcccg gaagcccaat cactcgcttg atgtaacttt
     1561 tcgacgggtc atctggaaac cagaagacga cgatgtctcc gcgatcgagt tttggagccc
     1621 accggtactc atcgtaatag atcagtttat taacaaagat gcgttcgccg tcgtgcaatc
     1681 ggggaagcat tgacgtaccc tcgaccttga ccggctgtac aataaacaca accacaaggg
     1741 ctgcaatcat aagtgcgaaa ataaggtcgc gaagcaaaag tctcgcttct gaccataaat
     1801 cgttgcgaga cacttcttcc ggacgatgaa tctgaatatc tttgtcgtcg tcgatgtgga
     1861 ttccgccaaa gggcggtggc tggcgcttgc gaattcctaa catagggatt gcattatatt
     1921 tcaaagttgg tcagtagtca gttgtccgtg gtccgtggtt agttgtccgt agttagttgt
     1981 cggttgttgc aggtaattca ggtatcacga atcttaataa cttttgatac tcgtgaagct
     2041 taacaccaca cggccaggtg gtgcctacat tgatggtgat tgacgtgcaa acaactagcg
     2101 acggacaact gaccacggac aagctccttg acagcctttg cgcccgtatc tatcatcctg
     2161 acctgcattt gcagcccaga agcatccgtg ccccagcgga gaaaggccat ctaaccgtga
     2221 tcaagttaga cattatcaat cgcgtcgctg acaaaacagg cgtaccgaaa atgaaagccg
     2281 aacaggcggt ggaggccctt ttccaatcga tgaaagaggc tctcaccaga ggcgagagaa
     2341 tcgaattgcg aggttttgga gtgtttgtcg tcaaaccacg caagcgcggt gtcggacgaa
     2401 atccacggac tggcgaggaa gtcgcgatac cgtccggtaa gactatccgg tttaaacctg
     2461 gcaaagaatt gcaggctcac gaagacggcg actcagaaga ttcgatttag cgctttcgca
     2521 gaactacgat attcaaaggc tagagatgga aggcatccac gctctagcct ttaatcttct
     2581 aaccaggcag catggcggaa actctcagaa cagcttttag tctttatccc caaccaggac
     2641 tgaaaccaac cgctcgggat tggcgattac acagttccct tctgttcctg acagcagtga
     2701 ccacaacgtt cgctggtttg atgttggtat cgccacagct gccgactcct gagcctgctc
     2761 tgaccaaccc cctcgactac ctgctttatc tacctcttag ttattactat tcagtagttg
     2821 aattgcttcg atattcgttt tcgcaccctt acctattagg tcaagctggg gcttttgcgg
     2881 ctagccttct ggctatcctg atgtcgcatg aaatgggcca ctacctggcc tgccgctatt
     2941 atggtgtcga tgcgacgctt ccttatttta ttccggcacc gccgctcttc ctggcaggaa
     3001 cgtttggcgc ttttattaag attcggtcgc ctataccttc gcgtcgagcg ctttttgata
     3061 tcgggctcgc cggtccgctt gccgggttta tcgtggcggt tcccgtggcc gtgatcggta
     3121 tactaaccat tggtccacct gtcacccagg ggtccggaat cgtcttcaac gatccgctcc
     3181 tgtttcggtt tgtcgctcga atgtttggag tacacctgga tccgcactcg actatcagcc
     3241 cttattacat ggccgcctgg attgggttgc tggtgacgag cctaaacctt atgccggtgg
     3301 gccaactcga tggtggtcac ggaacctttg ccgttttcgg cgagcaggta catcgaatcg
     3361 tgggcaaggt tgccttcgtt tgtaccggaa tcctggcgat tctaggattc tggtggtact
     3421 caagcccgag cggttttctt tatacaatcc tgttgggaat tatgttgaga gtccgacatc
     3481 cgcaaccagc cgtgatggag ccgttgggat cgaagcgagt tctgatagca attcttacgc
     3541 taatcgtatt cgtactctgt ttcgcgccat ttccaatcgc gattgactaa gggactaagc
     3601 gccagccgga agtaagcctt taatctctct aaccacgcgc gccacttcgt cctgattagc
     3661 gagggcaatc atgaagaact tatatccttg atggaaacag atcacaccat taccgaacag
     3721 ccaaacacca tccagaaacg ggttaccacc taagagccag ctgattgggg ctccggtgat
     3781 cgcgccaggt tgaaaaattt gcgcccacat tgcagctcca gcagcacagc tgaaagcgcc
     3841 gagcaacggt gcgccaacgt acttcacaat ttgccgctca aactctgctc gtttctgttc
     3901 ggcttccgaa agcgcacctg tcgcgattaa cttttcctgc tccttcaagt agctatcaaa
     3961 cttttcactt gcctttcgca atccataaac cggaagagcg gagtgcgtca gccagtgcgg
     4021 gtgcgagcct aagagcactg cgtctaaacc ggggaaagac gcgccagccc tgatacctaa
     4081 aaccagtgac accgaaacaa cggcagcttt gcctggattt tccttgatcc agtcgcgagc
     4141 tttcaacata ccaatggtgg cagccgccga cgctcgtgaa aattttgctc cggtaccgta
     4201 aaactgcgaa gcacgatcat agtaggttct ggccccttcg aagacctcgc ctgccttctt
     4261 gccagcgtcg cctgccgcat cacgaaaggt ttcactcgct ttctttacac cgcgcatcgt
     4321 ctcatttgcg gcttttctgg ctgcgtcacc aagttcctcg tcgctggaca atttttcggc
     4381 ttcggcgcgt agtcgttcag ccccgctcgc cacagtctcg acccctttac gggccgcgtt
     4441 ttcggccgct cgcgcacctt cgccaacgat atcagagagt ccaaacttct catttagctc
     4501 acgcgctttt cgcagcgtgg cttgctgcca ttcgtcgatt ctgtctttat aatccgccat
     4561 tcttaacctc tggacgctac ccgtcgatct cttgcaggat gtacgtcgcc cgcggtcccg
     4621 gagttcggtt gggctggctc aatggaaact acccggtttc taccggtctt ttttgcctca
     4681 taaagagcaa tatctgcggc cttaagaata gcaccagcgg tgtgcccgga cgagggacac
     4741 tcagccacgc caaagcttgc tgtaattccg ctggcagtcg gtatttcgag tgtgttgatg
     4801 gcagttcgca gacgttccgc gacctcaaga cccccttcca gacctgtttc aaccaataac
     4861 acggcgaact cctcgcctcc aatacgggcc gcgagatcga tgcc
//
LOCUS       DASSFP010000153         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1675030, whole genome shotgun sequence.
ACCESSION   DASSFP010000153 DASSFP010000000
VERSION     DASSFP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675030"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VFH91_03320"
     CDS             complement(<1..998)
                     /locus_tag="VFH91_03320"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET6862042.1"
                     /translation="MKLLIANGYVVDPSQQINGGKNVLIDEGRITAILSTHDPIPEDA
                     RVFDATGLIVAPGFIDLHVHLREPGQEYKETIASGSASAVAGGWTSICAMPNTDPIND
                     NAAVTRFIIDQGRAANLANVFPIGAATKGSAGKELAEMGEMKNAGIVAVSDDGHSIPS
                     AGMMRRVMEYANGHDLTVVDHCQDHSLSAGGVMHEGRWSLILGMRGMPAAAEEADVLR
                     DCVLAKLTGASVHVAHVSTRGAIEAVRRAKREGLRVTCEVTPHHWTLTDAAVAEYDTN
                     TKMSPPLRSQEHVEAVWEGMADGTIDTIATDHAPHHSDEKALEFDQAPFGIVGLETA"
     gene            complement(1023..2003)
                     /locus_tag="VFH91_03325"
     CDS             complement(1023..2003)
                     /locus_tag="VFH91_03325"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET6862043.1"
                     /translation="MQTGFNQRHLLGIRELNAAEITDLLDTAESFREISKREIKKVPA
                     LRGRTVINLFFEPSTRTRTSFEIAAKRLSADAVNVSVSTSSVSKGETLLDTARNLEAM
                     SPDCIVIRHSMAGAPQRLARMCNASIVNAGDGSHEHPTQALLDALTIREHKGKIEGLK
                     VAILGDILHSRVARSNIYLLTKLGATVSVSGPATLAPKEFGELVDSGLKIEKRFEDAI
                     EGADVVMVLRIQRERQDAAFLPSMREYAVHYGLQSRHLERAASDAIVMHPGPMNRGVE
                     ISSEIADGAKSLILDQVTNGVAVRMAVLYLLAGGSRADSTDVEDTPVKIS"
     gene            complement(2092..2655)
                     /gene="pyrR"
                     /locus_tag="VFH91_03330"
     CDS             complement(2092..2655)
                     /gene="pyrR"
                     /locus_tag="VFH91_03330"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET6862044.1"
                     /translation="MDFVEKARIMDGPRINRALARLSSEVVEENHGAGDLFLVGIRRR
                     GVPLAERMADKIADLEGSRPPVGVLDITLYRDDLSTVGAKPVVNRTDLPGDIENRNIV
                     LIDDVLYTGRTIRAALDQLIDFGRPKRVQLAVLIDRGKEHRELPIQADYIGKLVPTKK
                     SEIIKVMLQEFDEEEGVVIVERPSETG"
     gene            2786..3916
                     /locus_tag="VFH91_03335"
     CDS             2786..3916
                     /locus_tag="VFH91_03335"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET6862045.1"
                     /translation="MVYRSFLRPLLFSLPPETAHELALNSLVFIGSPAISKFVARHYQ
                     SNSFGELKRFGLSFPNPVGLAAGFDKDGIALQALCSLGFGFIEAGTLTFHAQPGNPRP
                     RLFRLPEDKALINRAGFNNDGAAAFVERIKRQRRNCILGVSIGKSKIAPLENAVEDYL
                     ASFEIVFPVADYVAINVSSPNTPQLRELQEKEQLSTLVTALKRRSNELEKESNRPAPL
                     PLLVKLAPDIADETLASIVDTLKTLKIDGIIATNTTISRSNLRASPAEIESCGAGGLS
                     GAPLRSPSTRMIAQLFRLTNGQVPIIGVGGIFNAEDAWEKICAGASLLQIYTGFIYEG
                     PGIAKQINDGLLKIMKREQVNHLDQAIGCRAEQLTSKQKGDQ"
     gene            3913..4650
                     /gene="pyrF"
                     /locus_tag="VFH91_03340"
     CDS             3913..4650
                     /gene="pyrF"
                     /locus_tag="VFH91_03340"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET6862046.1"
                     /translation="MSTRNKLIVALDVSPDDALKLVRELTGVVSTFKVGSQLFTQMGP
                     KIVEEILSTGSEVFLDLKFHDIPHQVAGAASSATGLGVSMFTIHASGGSEMMRRAVES
                     VKETAAQKQVKAPAVIAVSVLTSMDAAALADVGFSGTPEKNVLGLVKLAVASAVDGVV
                     ASPREAEAIRSAFPGQSLKIVTPGIRLEESETRDSEFKADDQKRIATPSMALAAGADF
                     LVVGRPITGAPKPAEAAQAIIAEMEEQ"
     gene            4725..>5449
                     /locus_tag="VFH91_03345"
     CDS             4725..>5449
                     /locus_tag="VFH91_03345"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET6862047.1"
                     /translation="MISNYYATVNLRLKQPVTCTLLILLFAIIIPVKAQENVGASSLP
                     LFSAPYKAGERLTYNVSYSNFPSVGHVEIRIAGRGTYFGRDAYQVKAHVQTTGVVSVA
                     LYSINNDYTTYIEAATGLPFRAEQVVRDEIRSTDSSQDLNQTPEAPEKRQQALSGTYD
                     LLSVFYRVRALPLTEGASYSFHVRGQSSDYQAEVKVNGHQTVRTNAGSFDTWVAQVRV
                     EGQSALKSFKVYFSDDERHVPVLL"
BASE COUNT         1336 a         1442 c         1347 g         1324 t
ORIGIN      
        1 gctgtttcca aacccacgat gccaaatgga gcctgatcaa attcgagtgc tttttcgtct
       61 gaatgatgtg gtgcgtggtc ggtcgcgata gtgtcgatag tgccatcagc cattccttcc
      121 caaactgctt ccacgtgctc ctggcttcga agcggaggac tcatcttcgt gtttgtatcg
      181 tactcagcaa ccgccgcgtc ggttagggtc caatggtgag gtgtgacttc acatgtcact
      241 ctcaatcctt ctcgtttcgc gcgccggaca gcttcaattg ctccccgggt cgatacgtgg
      301 gcaacatgaa cactcgctcc ggtgagtttc gcgagcacac aatctcgtag aacgtcagcc
      361 tcctctgccg cagcgggcat tccgcgcatg ccgagaatca aagaccaccg tccttcatgc
      421 ataactccac cggcacttaa cgaatgatcc tggcaatggt ccacgaccgt taagtcgtgc
      481 ccgtttgcgt attccatgac acgtcgcatc attcctgctg atgggattga gtgcccatcg
      541 tcagaaacag caacgattcc agcatttttc atctcgccca tttctgcgag ttctttgccg
      601 gcacttcctt tcgtagcagc tccgattgga aaaacgttcg cgagatttgc agctcgtccc
      661 tgatcgatta tgaatcgagt gaccgcagca ttgtcattta tgggatcggt gtttggcatg
      721 gcacagatac tggtccagcc gcctgcaaca gccgacgccg atccgctcgc gattgtctcc
      781 ttatattcct gacctggctc gcgcaagtgg acatgaagat caataaatcc cggcgccacg
      841 atcaatcctg tggcgtcaaa tacccgtgcg tcctcgggta tcgggtcatg ggtgctcagg
      901 attgccgtta ttcgaccttc gtcgattaag acattcttgc cgccatttat ctgctgagat
      961 ggatcaacaa cataaccatt ggcaatcagc agtttcattt ctttcgtcgc cttcctctcg
     1021 cgctaggata ttttgactgg ggtgtcctca acgtcagtac tatctgcccg actaccaccc
     1081 gccagcagat agagaaccgc cattcgtact gcaactccat tagtaacctg atcgaggatg
     1141 agtgatttgg ccccatctgc aatttcagaa gagatctcca cacctcggtt catcggacca
     1201 gggtgcataa cgatcgcgtc acttgcagct ctttcaagat gccgcgactg taaaccgtag
     1261 tgaactgcgt actctcgcat cgaaggaaga aaagcagcat cttgccgctc gcgctggatt
     1321 cggagaacca tcaccacgtc tgcaccttcg atggcgtcct caaacctctt ttcgattttt
     1381 agtccactgt ccacaagttc tccaaactct tttggtgcga gcgtagcggg tccggaaacg
     1441 ctgaccgttg ctccgagttt tgtaaggaga tagatattgg aacgagcaac tcgggaatgc
     1501 agtatgtcgc ccaaaattgc aacctttaat ccttcaattt tccctttgtg ttcacggatt
     1561 gttaaagcat ccaggagtgc ctgggttgga tgttcgtgtg agccatcgcc tgcgttcaca
     1621 atggacgcat tacacatgcg agcgaggcgt tgtggtgctc cagccatcga atgtctaatg
     1681 actatacaat caggagacat cgcttccaga tttcgtgcgg tatcgagcag ggtttcgcct
     1741 ttggaaacgc tggaggttga tacgctgaca ttaaccgcat cggccgaaag gcgctttgcc
     1801 gctatttcga aagaagttct ggtccgagtc gaaggttcaa agaatagatt aatgacagtt
     1861 cgaccgcgca aagccggtac tttcttgatc tcacgtttag agatttcccg aaagctctcc
     1921 gcagtgtcca aaagatcggt tatttcagcg gcattgagct cgcgtattcc aagcagatga
     1981 cgttggttga atccagtttg catcgtcggt ttcttacctg gggaccaaat ttgatcggtt
     2041 gtccaaagaa attaggtcat aaaaaaagcg gcctgcaggc cgcttcgttt ttcaacccgt
     2101 ctcagacggt cgctcgacga taacgactcc ttcttcctcg tcgaactcct gcaacattac
     2161 tttgatgatc tcgcttttct tagtaggaac cagtttcccg atgtaatcag cctgaatggg
     2221 caactcccga tgttctttgc cgcgatcaat aaggacggca agctgaaccc gtttggggcg
     2281 cccaaaatcg atgagctggt cgagggctgc tctaatcgtc cgaccggtgt agagcacatc
     2341 gtcaattaaa acgatgttgc gattttcgat gtcgcccggc aggtcagtac gatttacgac
     2401 tggtttcgca cctactgtcg aaaggtcatc acgataaagc gttatgtcga gtacgccaac
     2461 aggcggccga gagccttcaa ggtcagcaat cttatcggcc atccgctctg ctagcggaac
     2521 accgcgacga cggataccga ccagaaacag atccccggcg ccatgatttt cctcgactac
     2581 ctctgaagac aaacgggcga gggcacgatt aatacgtggc ccatccatga tgcgtgcttt
     2641 ctcgacgaaa tccatcggag cggatactag caagaagaat ttcgcgtgac aagtaacgcc
     2701 atagcgtgtc ttctcctaac ttgtcacaga atctgagtgt ctgttagtct gcaaacgttc
     2761 gcccactttt tgtaagcgcg actcaatggt ctatcgctca ttcttgcgac cgctactctt
     2821 tagccttcca ccggaaacgg cccacgaatt ggcattaaat tctttggttt tcatcggaag
     2881 tcccgcaatc agtaaatttg tagcccggca ctaccagtca aactctttcg gagaacttaa
     2941 gaggttcggg ctcagctttc cgaatcccgt cggactggca gccggattcg ataaagatgg
     3001 aattgcgttg caggcacttt gctccctggg cttcgggttt attgaggctg gaaccctcac
     3061 gtttcatgcg caaccgggaa atcctcgacc ccggttgttt cgcctgcctg aagacaaagc
     3121 gttgatcaac cgtgcgggct tcaataatga tggcgcagcg gcgtttgtcg agcgcatcaa
     3181 acgacaacgt cgcaactgta tacttggtgt cagcatcggc aagagcaaga ttgccccgct
     3241 cgaaaatgcc gttgaagact accttgccag cttcgagatc gttttcccgg tcgcagatta
     3301 cgtcgcgatc aacgtcagtt cacctaatac acctcaactg cgcgaattgc aggagaagga
     3361 acagctttca actcttgtaa ctgccttaaa acgccgaagc aacgaattag aaaaagaatc
     3421 gaacagacca gcaccgcttc cactactcgt aaagctcgct cctgacatag ccgacgaaac
     3481 tctcgcttca atagttgaca ccctgaaaac gctgaaaatt gacggcatca ttgctaccaa
     3541 taccaccatc agtcgttcga atctacgcgc atcgccggcg gaaatagagt cgtgtggcgc
     3601 aggaggattg agcggtgctc ccttacgatc gccgtccacg cgaatgatcg ctcaactctt
     3661 tagactaaca aacgggcaag tgccaataat cggggtaggc ggaatcttca atgccgaaga
     3721 tgcctgggaa aaaatttgcg cgggcgcaag cttgttacaa atctataccg gtttcatcta
     3781 tgagggtcct ggaatagcca aacagattaa cgatgggctg ctaaagatta tgaagcgtga
     3841 acaggtcaac catttggacc aggcaattgg gtgtcgggca gagcaactta cttcaaaaca
     3901 aaaaggagac cagtgagcac aagaaacaaa ttgatagtcg cattagacgt atcgccggac
     3961 gatgccctca aactagtgag ggaattgact ggagtggtat ccactttcaa agtagggtca
     4021 caactcttta ctcagatggg accgaagatc gtggaagaaa tactttcgac aggctctgaa
     4081 gttttccttg accttaaatt ccacgacata cctcatcaag tcgcaggggc agcctcatca
     4141 gctacgggcc tcggcgtttc aatgttcacc atacacgcaa gcggcggcag tgaaatgatg
     4201 cgtcgagccg ttgaatcggt aaaagaaacg gcggcgcaaa aacaagtaaa ggctccggca
     4261 gtgatagctg tcagcgtatt gaccagtatg gacgcggcgg cactcgccga cgtcggcttc
     4321 tcaggaactc cggaaaaaaa cgttttgggt ttagtaaaat tagctgtagc ctctgcggtc
     4381 gatggagtgg tagcttcacc tcgagaggcc gaagccatcc gctcggcatt ccctggtcag
     4441 tccttaaaaa tagtgactcc agggatcaga ctcgaagagt ctgaaactcg agatagcgag
     4501 ttcaaagccg atgatcaaaa gcgaatagca actcccagta tggctctcgc agccggcgcc
     4561 gacttcctgg tcgtaggcag accgattacc ggcgctccca aacctgccga ggccgcccag
     4621 gcgatcatcg cagagatgga agaacaataa cggcgaacga aaggaactcg tgcaaggggt
     4681 tgtgcgtgta gatttagtgt agtccagcgc tatttgccct actaatgatt tctaactatt
     4741 atgcgacggt gaatctcagg ttgaagcaac cggtcacttg cactctacta atcctccttt
     4801 tcgcgatcat cattccagtt aaggctcagg agaatgtggg cgccagttcc ctgcctctat
     4861 tttcggctcc ctataaggca ggcgagcggc tgacctacaa cgtctcgtat tcgaactttc
     4921 catctgtcgg ccatgttgag attcgaatcg ccgggcgggg tacatacttt ggacgggatg
     4981 cttaccaggt caaagctcac gttcaaacta caggtgtagt cagcgtcgcc ctttattcga
     5041 ttaacaacga ttacacgact tatattgagg cagctacagg actccctttc cgtgcagaac
     5101 aggtcgtccg cgatgaaatc agatcgaccg actcgtcaca ggacctaaat cagacgcccg
     5161 aagcacccga aaaaagacag caagcgttaa gcggcaccta tgacctgctc tccgtcttct
     5221 accgtgtcag agcgctgcct ttaacagagg gagcttcgta cagttttcat gtacgcggac
     5281 aatcgagtga ttaccaagct gaagtaaaag tgaatgggca ccagaccgtt cgcacgaatg
     5341 ctgggtcgtt tgacacctgg gttgcacagg taagggttga aggccagtca gcgttgaaga
     5401 gttttaaagt ctattttagc gacgacgaac gtcatgtgcc cgtgttact
//
LOCUS       DASSFP010000154         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_335848, whole genome shotgun sequence.
ACCESSION   DASSFP010000154 DASSFP010000000
VERSION     DASSFP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335848"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFH91_03350"
     CDS             <1..691
                     /locus_tag="VFH91_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6862048.1"
                     /translation="PSFSPAAMPQIFHHSTNALSKITIYGAVFIFVAALWVAVELNRS
                     SWNTGQWVERQQPVQFSHKHHVGDDGVDCRYCHTSVETSASAGMPSTNTCMNCHKQLW
                     ADSPYLEPVRASFRTGKPIEWIRVHDLPDFAYFNHSIHVNKGVGCSTCHGRVDEMPII
                     YQASTLQMEWCLECHRAPERFVRPKEHIFDMAWRPENKGEDQLNEGKTWKAQFHIQGK
                     EVLTSCSTCHR"
     gene            707..>2349
                     /locus_tag="VFH91_03355"
     CDS             707..>2349
                     /locus_tag="VFH91_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HET6862049.1"
                     /translation="MSDSDRHINFQLMRDHILATQEGKSGNAGKKYWRSLEELADSPA
                     FSEFVAREYPQHAEEWHDPIERRTFIKLMGASLALAGLSGCAFQPPEKIVPYVKQPEE
                     EVPGKSLFYATAAVLFGAATPVLVRSNEGRPTKIEGNPDHPNNRPGELNAEDPSKDKR
                     GSSATDIFTQASILSLYDPDRSQTISFREDIRTWTAFVGEMRGKLDEQRAKQGAGLRF
                     LTESIISPTLGDEMKTLLASLPQAKWHQYEPANRDNAREGAIMALGQPLNTMYRFDLA
                     DRILSLDNDFLSCFPGSLRYARDFMDRRRVLEKSSEMNRLYVVETTPSNTGAKSDHQW
                     VVKPSEFQAAVNTIAAALSVGTSGGPTANFAWVAPLVKDLQQHKGTSIVIAGDQQPPY
                     IHALAHAMNSALGNVGKTVLYSDPIDVNWVNHRESLQDLVKDLDAGKVDLLVIIGGNP
                     VYTTPVDLKLDFARLEKAKLRVHLSEYKDETSEVCHWHVPQAHYLESWGDARSYDGTV
                     SIVQPLIEPLYDGKTPLELLAVFSEQYDRKPYDIVKQYW"
BASE COUNT          582 a          597 c          610 g          560 t
ORIGIN      
        1 gccctcgttt agtcctgcgg cgatgccaca gatctttcat cacagcacca acgcgctgtc
       61 caaaatcacg atctatggcg cggtatttat ttttgtcgcg gcgctatggg tcgcagtcga
      121 actaaaccgc tcttcctgga ataccggcca atgggttgag cgtcagcagc ccgttcaatt
      181 tagccataaa catcacgtag gagatgacgg cgtcgattgc cgttattgtc atacctctgt
      241 tgagacttca gcctcagctg ggatgccctc taccaatacc tgcatgaact gtcacaagca
      301 gttgtgggcc gatagtccat acctcgagcc ggttcgcgca agttttcgca caggtaagcc
      361 gattgagtgg attcgcgttc acgatctgcc tgactttgct tacttcaatc acagtatcca
      421 cgtcaataaa ggtgttggct gctcgacttg tcatggacgc gtcgatgaga tgccaatcat
      481 ctatcaggcc tcgactctgc aaatggagtg gtgtcttgag tgtcaccggg cgcccgagcg
      541 cttcgtcaga cccaaagagc acatttttga tatggcctgg cgccccgaaa ataaaggcga
      601 agaccaacta aacgaaggca agacgtggaa agcgcagttt catattcaag gcaaagaagt
      661 gttgactagt tgttcgactt gccatcgata gatagcgaat gagcgaatga gcgacagtga
      721 tcgtcacata aattttcagc tgatgcggga ccatattctt gcgacgcaag aaggaaagag
      781 tggtaacgcg ggcaaaaagt attggcgaag tttagaggaa cttgctgata gtcccgcctt
      841 ttctgagttt gttgcgcgcg agtaccctca acacgctgaa gagtggcatg acccgattga
      901 gcgacgcacc tttatcaaat taatgggtgc ctcgcttgct ttggctggat taagtggttg
      961 cgctttccag cctccagaaa agattgttcc ttacgtaaaa caaccggaag aagaagttcc
     1021 tggtaagtct ctcttctatg ctactgctgc ggtgctgttc ggagcggcta cgcccgttct
     1081 ggttcgcagc aacgaaggta gaccgaccaa gatcgaaggc aatccggatc acccaaacaa
     1141 tcgacccggc gaactcaatg cagaagatcc gtctaaagat aaacgcggct caagcgctac
     1201 agatattttc acgcaagcct caatcttaag tctctacgat ccggaccgat cgcaaaccat
     1261 tagttttcgc gaggacattc gcacttggac cgcgtttgtc ggagagatga ggggtaagct
     1321 cgacgaacag cgggcaaagc aaggtgcggg tctgcggttt ttgacggagt ccattatctc
     1381 gccaaccctt ggcgatgaaa tgaagacgct gctggcgtct ctaccgcagg ctaagtggca
     1441 tcaatacgag cccgccaatc gcgacaatgc ccgtgaaggc gcaatcatgg cgctgggcca
     1501 gccgcttaac acgatgtatc gattcgatct tgctgatcgg atcttgtcgc ttgataacga
     1561 cttcctgtca tgtttccccg gttcgctgcg ctacgcgcga gatttcatgg atcgccggcg
     1621 agttctcgaa aagagctcag agatgaatcg tctctatgtt gtggagacga ctccaagtaa
     1681 cacgggagca aagtcggatc atcaatgggt agtaaagcca agtgaatttc aagctgccgt
     1741 caacacgata gcagcggcat tgtcggtcgg taccagtggt ggtcccactg caaatttcgc
     1801 ctgggtcgcc ccattagtaa aggatcttca acaacacaaa ggtacaagca tagttattgc
     1861 cggcgaccag cagccaccgt acattcatgc gctcgcacat gcgatgaata gcgcgttagg
     1921 aaatgttgga aagacggttc tctactccga tcctatcgac gtcaactggg taaaccatcg
     1981 cgagtcgtta caagatctgg ttaaggacct cgatgctgga aaggttgatc tgctggtgat
     2041 tattggcggt aaccccgttt acaccacgcc ggttgatttg aagttggatt tcgcacgact
     2101 cgagaaggcg aaacttcgag ttcaccttag cgagtacaag gatgaaactt ccgaagtttg
     2161 ccactggcac gttccgcagg ctcactatct cgagtcctgg ggtgatgccc gttcttacga
     2221 tggcacagtc tcgattgtgc agcctttgat cgagccgctt tacgacggca aaaccccgct
     2281 tgaactgctc gcagtttttt ctgagcaata cgatcgaaaa ccgtatgaca tcgtcaagca
     2341 atactggca
//
LOCUS       DASSFP010000155         5276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_336101, whole genome shotgun sequence.
ACCESSION   DASSFP010000155 DASSFP010000000
VERSION     DASSFP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336101"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VFH91_03360"
     CDS             complement(<1..1487)
                     /locus_tag="VFH91_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6862050.1"
                     /translation="MKPLLLLIFLLGTVSSAGAQQVGLITNTDGRKTVSLDGQWQTII
                     DPYESGYYDYRYQPSSNGFFKDAKPKTKSDLIEYDFDKSQTLKVPGDWNTQADHLIFY
                     EGTIWYKKSFDYQRKPNTRIFVYFGAANYLTDVYLNGEKLGQHEGGFTPFNFEITNLV
                     RDTGNFLIVKVDNKRRRDAVPTLMTDWWNYGGLTRQVKLLETPATFVQDYFIQLKKGS
                     REQITGWVKVNGDKPNQKVTIRIPEAAIDKSFTTDANGLAMINLTAALKLWSPENPKL
                     YDVTIASETDRVQDQIGFRSIETRGSQILLNGSPIFLRGVCIHEEAPLRGGRAYSRED
                     AQILLGWAKELGANFVRLAHYPHNEFMVREADKMGIMVWSEIPVYWTILWENPATLEN
                     ARNQLTEMITRDRNRAAVIIWSMANETPLSDARLSFLKNLVEHARSLDSGRLISAAME
                     RHYTDEHTQMIDDPLGEYLDVLGCNEYVGWYDGLPDKADRLEWKT"
     gene            1897..2730
                     /locus_tag="VFH91_03365"
     CDS             1897..2730
                     /locus_tag="VFH91_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6862051.1"
                     /translation="MSGSDSFNERKLDGKSLRRSESHYRAVAEATSDGIITINSQSTI
                     LLVNPAAEKIFGYTTRELLGRQLTMLMPEYHRHLHRQGIARYLETGNRHLEWGAVQLP
                     GLHKSGEEIALEISFLEFVKDGQRFFTGIVRRVAERNHTREVIRDDHELLRAIVNQTS
                     VGISVVDPGGRFTFANEKYCQIVGRTSDELLKIKMADITHKDDLRENHSLFERAIANG
                     EGFEIEERYVRPDGTCVWVHNCVTVLHDANGEQWGVMAVTLDLTKSIESSSTDPALHS
                     T"
     gene            2888..3208
                     /locus_tag="VFH91_03370"
     CDS             2888..3208
                     /locus_tag="VFH91_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862052.1"
                     /translation="MFTRHVIMELKPNAIKDLANTVESKILPLLAKQKGFRDEITFVA
                     PDRSEAIAISFWDTKEEAEAYNVTGYPEVLKILAGMVEGTPRVGTAEVLTSTFQKLAA
                     AKTA"
     gene            3323..3781
                     /locus_tag="VFH91_03375"
     CDS             3323..3781
                     /locus_tag="VFH91_03375"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6862053.1"
                     /translation="MKKLTGMLMLTFVVVFVSAIATAQTKIKLTDEQALIALDKQWTA
                     AELRLNADDQKTISRIVAEDYTGTTPFGQVENKAQYIAALKATPDTDVADEYVVHFFD
                     GGKLAIMSHRGTVNGKPTYRSTHVWVKRGSNWELLQNHVSNIGEPTIATK"
     gene            complement(3928..4566)
                     /locus_tag="VFH91_03380"
     CDS             complement(3928..4566)
                     /locus_tag="VFH91_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6862054.1"
                     /translation="MGMKKPVKKKIRRSEGGGPGGLRLTIADLVILSLLAERPMHGYE
                     VNATLEDRKIRDWAAVSRPQIYYSLDKLTELGFIRVTVEGSPSAGPERRVLDTTESGR
                     KRLADALEDTHWTSTHVHQPFLTWLALSWQARPRAFQKQLKQRHRFLEQKLAEEQSTL
                     QDVLKEVGHPYHEAVWMLKLATAQIELELKWLEGIERESPKRAPAQRTRKHP"
     gene            4730..5119
                     /locus_tag="VFH91_03385"
     CDS             4730..5119
                     /locus_tag="VFH91_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6862055.1"
                     /translation="MINKSTPVLHVKSVEPSVKFWTERFGFKKTIEVPEGDHLGFVAL
                     ENETVEVMYQTYSGMQSDPENPLAKAADQGPSFLFMEVGDISSVANALNGAEIVQPIH
                     ETFYGSKEIVVKEPGGHFVIFAQMPGH"
BASE COUNT         1321 a         1304 c         1358 g         1293 t
ORIGIN      
        1 ttcgtcttcc attccaaccg atcagctttg tcaggcagac catcgtacca accgacatat
       61 tcgttacatc ccagcacgtc aaggtattct ccaagcggat catctatcat ctgggtatgc
      121 tcgtcagtgt agtgacgttc cattgccgct gatattaatc gcccggaatc aagactcctg
      181 gcgtgctcaa ccagattttt aaggaacgac aagcgtgcgt cactcagggg ggtctcattg
      241 gccatggacc aaataataac cgcagctctg ttcctgtcgc gcgtaatcat ctcagtcagt
      301 tgattccgag cattttccag tgttgccggg ttttcccaaa gtatggtcca gtaaaccggt
      361 atctctgacc acaccataat ccccatcttg tcggcctccc gaaccatgaa ttcgttgtgg
      421 ggataatgtg cgaggcggac gaaatttgcc cccaattcct tggcccatcc caggagtatt
      481 tgcgcatcct cgcggctgta ggcacggccc ccccgaagcg gtgcttcttc atgtatgcac
      541 acgccgcgga ggaagatggg gctgccattc agcagaatct gggaacctcg cgtttcaatg
      601 ctacgaaaac caatctggtc ctgcacacga tcagtttccg atgcaatcgt gacatcgtag
      661 agctttgggt tctcgggaga ccacagcttc aatgccgcgg tgagattgat catggccaat
      721 ccgttcgcgt cagttgtaaa ggatttatca atggcggctt ctgggattcg aatagtcact
      781 ttttggttgg gcttgtctcc gttaactttc acccagccgg tgatctgttc gcgcgagcct
      841 tttttaagtt gaatgaagta gtcctgtaca aaagtagcag gcgtctccag aagcttcacc
      901 tggcgagtga gtcctccata gttccaccaa tctgtcatca atgttggcac agcatcgcgc
      961 cgccgtttgt tgtcaacttt gacaatgagg aaattacccg tgtcacgaac tagattggtt
     1021 atttcgaaat tgaaaggagt gaagccgcct tcatgttggc cgagcttttc accattgagg
     1081 tagacgtcgg ttaagtagtt agcggcgcca aaatatacaa agatacgagt gttcggcttt
     1141 cgctgataat caaaggattt cttgtaccag attgtgccct cgtagaagat cagatgatcc
     1201 gcttgcgtat tccagtcgcc gggcacctta agtgtctgtg acttgtcgaa gtcgtattca
     1261 ataagatcac ttttcgtttt aggcttcgcg tctttgaaga aaccattgga gctcggttga
     1321 tagcgataat cgtaatagcc ggattcgtaa gggtcgataa tcgtttgcca ttgaccatca
     1381 aggctaactg ttttgcgacc atccgtgttt gtgattaagc caacttgttg cgcgcctgct
     1441 gaagagacag tgccgagcag aaagatgaga agaagtaatg gtttcatttg tgaactcgta
     1501 actacatatg catcgtgctc ttcaaccagc tggctgaaga gcacgaatgg tcgctatgat
     1561 tgtcggttag gtaagtcaga agtctggttt tcactcgtca ggttcttctt gagagtaaag
     1621 gaaacctgag gaatctatgt caagacggtt aagttctagt acgaataatt gcggcgtgcg
     1681 aggcataatt tgtcagaaca cctggaccgt gtcaataaga aaatcgagtt tctaaggtgt
     1741 taaggtgagc gcggtccatc ctcgagtgga tttttctaag cccattcgat tcattgacga
     1801 gtatcgaccc aatccataga atcctcatct gtacaaataa tattgttcgt tcccgtcaag
     1861 tagttgattt cttgatctaa gctgcccaca tttcccatgt ctggttctga cagtttcaac
     1921 gagcgtaagc tcgatggtaa atcgttgcgc cgcagcgaaa gccactatcg agctgttgcc
     1981 gaagccacta gcgatggcat catcaccatc aattcgcaga gcactattct gctcgtcaat
     2041 ccagcagcgg aaaaaatttt cggctacaca actagagagc tgctcggccg ccagctcaca
     2101 atgctgatgc ccgaatatca ccggcacctt caccgccaag gcatagcgcg ctacctcgaa
     2161 acgggaaacc gacatcttga atggggagca gtgcagctgc ctggtctgca taagagtgga
     2221 gaagaaattg ctctcgaaat atctttcctg gagttcgtca aggacggcca acgtttcttc
     2281 accggcatcg tccgaagagt cgcagagcgt aatcacacca gggaagtcat tcgtgatgat
     2341 catgagttgt tgcgggcgat agttaaccag acgagcgtcg gtatttcggt tgtggatcca
     2401 ggaggtcgtt tcacctttgc gaacgaaaaa tattgtcaga ttgttgggcg caccagtgac
     2461 gaactgctca agatcaaaat ggcagacatt acacacaagg acgatctacg tgaaaaccat
     2521 tcattgttcg agcgcgcaat tgctaatggt gaaggcttcg agatcgaaga gcgttacgtg
     2581 cggccggatg gtacatgcgt ctgggtacac aactgcgtga cagttctgca cgatgctaat
     2641 ggcgagcaat ggggtgtaat ggcagtaacg ttagacctta caaagagcat agagagctct
     2701 tcaacagatc cagcgcttca tagcacttag ccggaggaca ctactgaatg tcacgcgggt
     2761 taggcaactg ggtaggcgcg tgataaggta cacagcgaac cgagttccaa cttactctct
     2821 tctcagctcc tctggaatca cccagatcac ctgaagtcaa cagccctaac aatctaagga
     2881 gctactaatg tttacacgac atgtgataat ggaactgaag cctaacgcca tcaaggattt
     2941 agccaatact gttgagagca aaatcttgcc acttctcgcc aaacaaaagg gatttcgcga
     3001 tgagatcacc tttgttgcgc ccgatcgctc ggaagctatt gctatcagct tctgggacac
     3061 gaaagaggag gcggaggcct acaatgtcac tggatatcca gaagtgctga agatcctcgc
     3121 cggaatggtt gaaggaacgc ccagagtggg aacggctgag gttctgactt ccacatttca
     3181 gaagctcgcc gctgccaaaa ccgcgtaaaa gcggtcacca tggccccgca aatttgcgca
     3241 ccaactgaaa cagcagaagg tgcgcaaaaa tcaaaaccct cgactctgtc gagcctcacc
     3301 cacaacgaaa ggtcttgcag taatgaaaaa attgacaggt atgttaatgc tcacgtttgt
     3361 tgtagtcttt gtatcagcaa tcgctaccgc acaaacgaag attaagctca cggatgaaca
     3421 ggcgttgatt gctttagaca aacagtggac ggccgctgag ttacggctta atgccgacga
     3481 tcagaagact atcagtcgca tagtagctga agactacaca ggtaccacgc cattcggtca
     3541 agtcgaaaac aaggcgcagt acatcgcggc actcaaggcc actccggaca ctgatgtggc
     3601 cgacgagtac gtggtccact tcttcgacgg gggcaagcta gcgatcatgt cgcaccgcgg
     3661 gacggtcaac gggaagccga cctaccgcag cacgcacgtg tgggtgaaac gcggaagtaa
     3721 ttgggagctc ctccaaaacc acgtcagcaa catcggagaa cccaccatcg ctaccaagta
     3781 ggtaacgcgt cgcaggcgtt agctggttta gtaatccgac aaacgggcag taagtgatca
     3841 ttcacttgct gcccgtaatt atttgatttt gttgtggcgg ggactgaaag tgaaatgatt
     3901 gggatgtcta cgagttgcct attggagtta agggtgtttg cgtgttctct gcgccggtgc
     3961 tcgcttcgga gattctcgtt cgatcccctc aagccacttt agttccaatt ctatctgggc
     4021 agtggcgagt ttgagcatcc agacggcctc gtgatatggg tggcccacct ccttgagtac
     4081 atcctggagg gttgactgtt cctcagcgag cttttgctcg agaaatctgt ggcgctgctt
     4141 tagctgtttt tgaaatgcac gcggccgcgc ctgccatgag agtgccagcc aggtaagaaa
     4201 cggctggtga acatgagtgc tggtccagtg tgtgtcctca agcgcgtcgg ccagccgctt
     4261 gcgaccggat tcagttgtgt ccagtacgcg gcgctccggc ccagctgagg gtgaaccttc
     4321 caccgtgacc cgaatgaatc caagctcagt cagtttgtcg agcgaataat agatctgggg
     4381 gcgcgacacg gcggcccaat cgcgtatttt tcgatcctcc agtgtcgcat tcacttcata
     4441 gccatgcatt ggacgctcgg ccagcagact gagaatcacg aggtccgcga tcgtcaggcg
     4501 caagccgccg ggccctccgc cctcgctcct ccttattttc ttcttgacag gtttcttcat
     4561 gcccattact atacactagt ctaatttaga ctagataaga aaaaaaacct aaacgcgaga
     4621 taaagccatg aaaaaggaaa ggactatgag ggttgcagcg gcggtcgggt tggcgttggt
     4681 gatttggacg gcgtttaatg cggcccgcgc aaaccagagg agagcaacga tgatcaataa
     4741 gtcaacaccc gtgctacatg taaagagcgt tgaacccagc gtgaaatttt ggaccgaacg
     4801 cttcggcttc aagaagacaa tagaagttcc cgaaggcgat catctgggct ttgtcgcgtt
     4861 ggaaaatgag accgtcgagg tgatgtatca aacttattcc gggatgcagt ctgaccctga
     4921 gaacccgctc gccaaagcag cggaccaggg tccgagtttt ctgttcatgg aagtgggtga
     4981 tattagctca gtcgccaatg cccttaatgg tgccgagatc gttcaaccga ttcatgagac
     5041 gttctatgga tcgaaggaaa tcgttgtgaa agagcccggc ggccattttg tgatcttcgc
     5101 gcagatgcca ggccattaaa gagtgcagag ttacgcagcc cagaaggtag tgttttcgct
     5161 cttaactccg ttaggagttg catgtttata gcccgagtca ctcactacct cgtgccgttc
     5221 gaaggggcgg aacgaaccta actgatcact atgcacttct atttgacatt aagcgc
//
LOCUS       DASSFP010000156         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1675485, whole genome shotgun sequence.
ACCESSION   DASSFP010000156 DASSFP010000000
VERSION     DASSFP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675485"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..565
                     /locus_tag="VFH91_03390"
     CDS             107..565
                     /locus_tag="VFH91_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET6862056.1"
                     /translation="MRFRVIWPGKTRDARLRSLVEDYVERLGHFVRVEIVELKEAGRA
                     DKPGIDKEAGRISDALRPGGVTVLLDVAGVEWTSQQLAEQVKTWEVRGIKEVAFVIGG
                     PHGLSQELVARADRRWSLSRLTLTHEMARVLMFEQLYRAYTIINGLPYQK"
     gene            614..982
                     /locus_tag="VFH91_03395"
     CDS             614..982
                     /locus_tag="VFH91_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET6862057.1"
                     /translation="MDKRRTKVFRDKLLDRREGLVGQVQAAELYSRERDAEATQDPAD
                     MAANAYTKELLVSMSDNDRQLLNLIDEALERIETTGYGKCVHCEEALPEKRLEAVPWA
                     RLCVRCQDLQERGLLSDDDD"
     gene            1052..1795
                     /locus_tag="VFH91_03400"
     CDS             1052..1795
                     /locus_tag="VFH91_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862058.1"
                     /translation="MFLTKTSSLVDALFSVAYPQDCRICEREVERRHLGVVCNECWKS
                     TRLFTEFDTICSKCGVLLPFPKQLESKELSCRTCESQLFDAARACGLYEKALRESVLE
                     LKRRPNIARHLRLLLQAVAHQPPFHKSSRIVPVPLHPLRERTRGFNQAVVIARTISSA
                     IGVPVDDHSLVRTHYSEKHRAGLDAQGRSDTVSNAFAVTHQRSIKDENILLVDDVLTT
                     GATANSCATALFEAGASTVCILTIARSAR"
     gene            2473..2664
                     /gene="tatA"
                     /locus_tag="VFH91_03405"
     CDS             2473..2664
                     /gene="tatA"
                     /locus_tag="VFH91_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6862059.1"
                     /translation="MTENMLAIGGLGTPELVVIAIVIFLLFGATRLPQLAKALGQSKR
                     AFKEGLDEGEKESRKNLED"
     gene            complement(2692..>3210)
                     /locus_tag="VFH91_03410"
     CDS             complement(2692..>3210)
                     /locus_tag="VFH91_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862060.1"
                     /translation="DDNQYDLVIGEASLVARERVRDMFKEMVRVAAPGGRVALTLTTA
                     SSFGEFFSIFWEALHNCEFIDLESKVERLIMGLPTVTDVEKMAENEGLDDVESWTRIE
                     EFGYESGDQFLKSPLVSEFLMKDWLASVPEALREKVSNEISRLINEERHEAEFTLSVK
                     ATLMVGRKALTH"
BASE COUNT          799 a          763 c          855 g          793 t
ORIGIN      
        1 ggtaacaaga ctgagtgcgc tcgaattctc ggcattagtc gcaaaaatct ctacgagaaa
       61 attgcgaagt acaaactcgg ttagattcgg ttaggcgatc tttttgatgc gttttcgggt
      121 tatttggcct ggcaaaacac gcgacgcaag actccgctca ttagttgagg attacgtcga
      181 aaggcttggc cattttgttc gagtcgagat cgttgaattg aaagaggccg gcagagcaga
      241 caagccaggc atcgataagg aagcgggccg catctcagac gctcttcgtc caggcggcgt
      301 tacggttctt ttggacgtgg ccggcgtaga gtggacgtct cagcaactgg cggaacaggt
      361 caaaacctgg gaagtccgag ggatcaagga ggtggcgttt gttatcggcg ggccacatgg
      421 cttgtcgcag gaattggtcg ccagggctga tcgacggtgg tcgctctccc gtttgacctt
      481 aactcatgaa atggcgcgcg tgctgatgtt tgagcagtta tatcgagcgt acacaattat
      541 caacgggttg ccgtatcaga aataacgaac tacccctaaa caattcaagt agggtggagg
      601 acgaaaacaa ataatggata agaggcgaac gaaagttttc cgcgacaaat tattggaccg
      661 gcgcgaaggc ctggtgggac aagttcaggc cgccgagtta tacagtagag agcgcgatgc
      721 cgaagctacg caggatccag ctgacatggc tgcaaacgct tatacaaagg aactgctggt
      781 gtcgatgtct gataacgaca ggcagttact gaatctaata gacgaggctc tcgagcgaat
      841 cgagacgact ggatacggaa agtgcgttca ttgcgaagag gctctacccg aaaaacgact
      901 tgaggctgtg ccgtgggctc gcctttgtgt tcgttgccag gatctacaag aacgtggatt
      961 gctttcggat gacgacgatt agccaatagc aactatttga tctagatttg tggcctcttc
     1021 agatttttct ggggaggtct ttttattttc catgttcctc accaaaacat catcattagt
     1081 tgatgcgttg ttcagcgttg cctatccgca agattgccgt atttgcgagc gagaggttga
     1141 gagacgacat cttggagtgg tttgcaatga gtgttggaaa tcgacccggc tctttaccga
     1201 gttcgatacc atctgctcga aatgcggagt actactacca ttcccaaagc agctggaatc
     1261 gaaagaactg agctgtcgga cttgcgaatc tcagttgttc gacgcggcgc gggcctgcgg
     1321 gctttatgag aaagctttac gagagtcggt gttggagttg aagcgacggc caaatattgc
     1381 ccggcacctt cgattgttac tgcaggcagt cgcgcaccaa ccgccattcc ataagagtag
     1441 tcgcatcgtg ccggtaccgt tgcatcctct gcgcgagcgc acgcggggct ttaatcaggc
     1501 ggtagtgatc gctcgcacaa tatcttcagc cattggagtg ccggtcgatg atcactctct
     1561 ggtaagaaca cactattcag aaaaacacag ggcgggtctc gatgcccaag ggcggagcga
     1621 cactgtcagc aatgcttttg cggtcactca tcaacggtca ataaaggacg aaaacattct
     1681 actggttgat gacgtgttga cgacgggcgc cacggcaaac tcttgcgcga cagcattatt
     1741 tgaagcgggc gccagcactg tttgtattct gaccatagct cgctcagccc gttgatttat
     1801 ctgggtggac aagcatcaac ctgaccggtt tcgggcatct gaagcaggac aggcgaacta
     1861 aattttgaca ccctattcca cgctcgcgta atatcagacc ttgtatgaac ggcagagtcc
     1921 tagttctaaa cacttccttt gaacccatca acgtgtgcac tgaaaggcgc gcggtcgtga
     1981 tgattttcaa aggggtggcc cgggtggaag agcacaatgg gcatatgctt cattcccaaa
     2041 aactgtcaat tcatgcacct tcagtaattc gtcttacaga atacattcat ataccttttg
     2101 aacgccgttc tctgtcccga aagaacatac ttttgaggga tcactcgacc tgtcagtact
     2161 gcgggaaaca gtatccgcct tcagagttga cgttggatca cgttcagcct cgctcgcgtg
     2221 gaggtgagtc ggcgtgggat aatctagttg cttgttgcaa acgctgcaat cataagaagg
     2281 gcaataggac ccctgaagag tcggggatgc atctgcttcg gaggcctcgc ggtttctctt
     2341 tacacgtaaa tcgtcagata atgcgttacc tggggcgcgc agatgaaacc tggaggaagt
     2401 atttgtttta cgagtcagat catgggaact agatagtccc tccggttaaa gcctccaaga
     2461 aagaggaaaa caatgactga gaacatgttg gcgattggag ggctcggaac tcctgagctc
     2521 gttgtgatcg caatcgtaat ttttctgctg ttcggggcca cacgcttacc gcaattagcc
     2581 aaggctctcg gacaaagcaa gcgtgcattt aaagaaggtc tggatgaggg cgagaaggag
     2641 tcgcggaaaa atttggagga ctaagttttc cgcccccttg atagagacaa tctagtgagt
     2701 gagggcttta cggcccacca tcagcgtagc ctttaccgat aaagtaaact cagcctcgtg
     2761 tcgttcctca tttatcaggc gcgagatttc atttgagact ttttctctca acgcttctgg
     2821 gacggacgcc agccagtctt tcattaaaaa ctcggagact agcggagact tgagaaactg
     2881 atctcctgat tcgtatccga attcttcaat tcgtgtccac gactccacgt cgtcgagccc
     2941 ttcattctcc gccattttct cgacatcggt aactgttggt aagcccataa tcaaacgttc
     3001 tactttcgat tcaagatcga tgaactcaca gttgtgtagt gcttcccaga agatagaaaa
     3061 gaactcgcca aagcttgaag ctgttgtaag ggttaaagcc actcgtccgc ctggtgccgc
     3121 gacgcgcacc atctccttaa acatgtcgcg cactcgctct cgtgcgacaa gtgatgcctc
     3181 tcctataacg agatcgtatt ggttgtcgtc
//
LOCUS       DASSFP010000157         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_776906, whole genome shotgun sequence.
ACCESSION   DASSFP010000157 DASSFP010000000
VERSION     DASSFP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776906"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFH91_03415"
     CDS             <1..819
                     /locus_tag="VFH91_03415"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET6862061.1"
                     /translation="KIKSGELKAELAATEFIKPAIAENSPPPTATPRPTPAPTPNAAP
                     VAELNSDLPFAAGEQLNYQIYLAGSNAPMGTATFQVRGISKYFDRTGLLLAVKAETTG
                     AAARVFVASDQIESYVDPKVLLPYRTVLNLVEGKRRRNQTLTINQDAGTATTDKGTRL
                     EIPVGTHDYVSFFYAVRTFRLNPPQQNAISILVENKPKTLIITSSKRETIQIGDQKIV
                     AIPVTLTTDDPQSDKYQFRMWLSDDKRRLPLRITAATQLGAIRADLAILPIASH"
     gene            871..1845
                     /locus_tag="VFH91_03420"
     CDS             871..1845
                     /locus_tag="VFH91_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET6862062.1"
                     /translation="MAQQVRILENHLAEIEIYFQLIYTASRRPGSTLKIVDEGRPNML
                     NSFVIVLREGFESFLLVAVILSYLRKANQKWLASSVYVAIAAALAASLGLAYLLKTGV
                     DDAVLERYLGATVGGYVSQFFANEALREAVLGVIAIVMVGSLVIHMWRTGPKVQQRMR
                     ERLSAMSSRSSRVAAVAGVFLFTFLMITREGMETALLLMQVRDSQLVQGAVLGLVAAG
                     TFAWGWARYGHLINVRRFFQVTGIFLLLFMIQVAIYSFHEFAEAGLLPNSEVLHTATE
                     KFSPDGVYGKWFSPLMIGICGLWLFGAWLVDRAKETSVSTLPKLEGTS"
     gene            complement(1842..>2751)
                     /locus_tag="VFH91_03425"
     CDS             complement(1842..>2751)
                     /locus_tag="VFH91_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6862063.1"
                     /translation="LTIMPLFHMNAVSVTTMSALYAGGSTVVSPKFSASRFWKIISDY
                     QITSFGSVATMLSMLLSTYPDGVPDGLTTDQLRFAMCGSAPVPAEVIKRFEETFGCLV
                     IEGYGLSESTCRSTFNPPDLNRRQGSCGLPIGNEMRVVDEDDNEVKDGTLGEIVLRGE
                     NILKGYYKNPTATAEAFRNGWFHTGDIGYRDSDGFYYIVDRKSDMIIRGGENIYPREI
                     DEVLYQHPAVASAAAVGMPDDLYGEEVTAFVVVKDATDVTAEELITFCKERLADYKCP
                     KTIRLVKEIPKGPTGKLLKRELAKMI"
BASE COUNT          667 a          722 c          645 g          717 t
ORIGIN      
        1 aaaatcaaat caggtgagct aaaagctgaa cttgcggcca ctgaattcat caaaccggca
       61 atcgcagaga actcgccacc accaactgct actccacgac ctactccggc tccaactccc
      121 aacgcagctc cggttgcaga actaaatagc gatcttccct tcgccgcggg cgagcaattg
      181 aactatcaga tttatctcgc tggcagcaat gcaccgatgg gcactgcaac ctttcaggtt
      241 cgaggtattt cgaagtactt cgatcgcact ggattgttgc tagcagtaaa agcagaaacg
      301 actggagcag cagctcgagt cttcgtcgcc agtgatcaga tcgagtctta tgtagatcca
      361 aaagttctac tgccctatcg aacagtcctt aacctcgtag aaggtaaacg acgacgaaac
      421 caaaccctca cgattaatca agacgctggc accgctacta cggataaagg cacgcgtctt
      481 gaaattccgg ttggaacaca cgactacgtt tcattctttt atgctgttcg gacgtttcga
      541 ttgaatccgc ctcaacagaa cgctatttct attttggttg aaaacaagcc aaagactctc
      601 atcatcactt cgtccaaacg agagacaatc cagattggcg atcagaaaat tgtcgccatt
      661 cctgtcactc ttacaaccga tgatcctcaa agtgataagt atcaattcag gatgtggctg
      721 agtgatgata agcggcggct cccgttgcga atcacggccg caactcagtt gggtgcaatc
      781 cgcgctgacc tggcaatcct gcccatagcg agtcattaac ggtcattcga agtagaattc
      841 tgacccatct tccctgaaac tgcgttggtt ttggctcaac aggtaagaat ccttgagaac
      901 catctggctg aaattgaaat ctattttcaa ctaatctata ctgccagccg taggccgggg
      961 agtactctca agatcgtcga tgaaggtcgt ccgaatatgc tgaactcatt cgtaattgtc
     1021 ctgcgtgagg gcttcgaatc ctttttgctc gtcgccgtga ttctctctta ccttcgcaaa
     1081 gctaatcaaa aatggctcgc atcttcagtt tatgtcgcta tcgctgctgc tcttgccgct
     1141 agcctgggac tagcttatct cctgaaaacc ggagtcgacg atgccgttct cgagcgatat
     1201 ctaggtgcga cggtcggagg ttatgtcagt caattctttg caaacgaggc tctccgcgaa
     1261 gccgttcttg gggtaatcgc tattgttatg gtcggttccc ttgtaattca tatgtggcgg
     1321 accggtccga aagtacaaca acgaatgcga gaacgcctct ccgcgatgtc ttcgcgcagc
     1381 tcacgtgtcg ctgccgttgc cggtgtcttt cttttcacgt tcctgatgat tacacgcgag
     1441 ggaatggaaa cagcgctgct gttgatgcaa gtacgtgatt cgcagttagt tcagggagct
     1501 gtgttaggtc tggtcgcggc gggcaccttc gcctggggat gggccagata cggtcatttg
     1561 atcaatgtcc gccggttttt ccaggtgact ggaatctttc ttttgctctt tatgattcag
     1621 gttgcaatct actcattcca cgagtttgct gaagcgggac ttctgccaaa ctctgaggtt
     1681 ttgcatacag ctactgaaaa attctcgcca gacggtgtct acggtaaatg gttctcacca
     1741 ttaatgatcg gcatctgcgg tttgtggttg tttggggcgt ggctagtcga tagggcgaaa
     1801 gagacaagcg tttcgacttt gcccaaactg gaaggcacaa gctagatcat cttcgccagt
     1861 tcacgtttta gtagttttcc ggttggtcct tttggaatct ctttaaccag tcgaattgtc
     1921 ttcggacatt tgtaatccgc taagcgttcc ttgcagaatg tgatcaactc ctcggccgta
     1981 acgtcagtcg cgtctttcac aactacaaag gctgtaactt cttctccgta caagtcgtcc
     2041 ggcattccta ctgccgccgc cgatgccacc gccggatgct ggtataagac ctcatctatt
     2101 tccctcggat aaatattctc gccgccccga atgatcatgt ctgactttcg atcgactatg
     2161 taataaaacc catccgaatc tcgataacca atgtcacccg tatgaaacca tccattgcga
     2221 aaagcttcag ctgtcgcagt tggattcttg taataacctt tcaggatatt ctcgccacgt
     2281 aaaacgatct cgcccaaagt cccgtccttc acctcattgt cgtcttcatc taccacgcgc
     2341 atttcatttc cgatgggtaa tccgcaagat ccttgccggc gattaaggtc gggcggattg
     2401 aatgtcgagc ggcaggtcga ctctgagagg ccgtatcctt caatcaccaa acatccgaaa
     2461 gtttcttcga aacgcttaat gacttcggca ggcaccgggg ccgagccaca catggcaaac
     2521 cgcagctgat ctgtggttag accatctgga acgccgtcag gatatgtgct caagagcatc
     2581 gatagcatcg tcgccaccga cccaaaagat gtgatctggt agtcggaaat aatcttccag
     2641 aaccgagaag cggaaaactt cggactcacg acggttgagc cgccagcgta gagcgcagac
     2701 atcgttgtca ccgagactgc gttcatatga aagagcggca tgatcgtcaa c
//
LOCUS       DASSFP010000158         5545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2122283, whole genome shotgun sequence.
ACCESSION   DASSFP010000158 DASSFP010000000
VERSION     DASSFP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2122283"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFH91_03430"
     CDS             <1..966
                     /locus_tag="VFH91_03430"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6862064.1"
                     /translation="AATDAEIARMKETLESGLKRGAVAVGLGIQYTPLASHWEILEMF
                     RVAKRYDASVHVHMRNAGVLEPASSVQALEEVIAAATITGASLHVVHISSTGGANTLK
                     LLQMIDEARARNLDVTTECYPYLAGMTDIKSAIFNEGWRDVFGIDYKDLQWAATGERL
                     TEETFERYRKTGGMVAMFSMTEEMVSGAVKSPLTMIASDGILENGKGHPRTAGTYSRV
                     LGKYVREQGALTLMEAITKMTLMPAQRLEKRVPSMKNKGRIKVGADADFAIFDPKTVN
                     DRSTYQEPAQYSEGIKFVIVNGEMVVKDGTLQSGVYPGRAIRAPF"
     gene            complement(1000..1887)
                     /locus_tag="VFH91_03435"
     CDS             complement(1000..1887)
                     /locus_tag="VFH91_03435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6862065.1"
                     /translation="MERKLASDYPQELLDLFHEYQHGDINRRTFLDRAGKFAVGGLTV
                     ATIFESLTPNYAWAQQVPPDDKRIKVGREVVESPAGNGSIKGYLARPSKNGKLPVVLV
                     IHENRGLNPYIEDVARRLAVANFIAFAPDGLTSVGGYPGDDEKGAAAFRTVDGKKMTE
                     DFVAAAKWLKARPDSTGKLGAVGFCFGGGIVNQLAVRLGADLNAGVAFYGRQAGADDV
                     PKISAPLLLHYAGNDQGVNAGIAAYEAALKANNKVYTVYMYDGKQHGFHNDTTPRYDE
                     AAAKLAWTRTLDFFNKYLR"
     gene            2009..3691
                     /locus_tag="VFH91_03440"
     CDS             2009..3691
                     /locus_tag="VFH91_03440"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HET6862066.1"
                     /translation="MDRKSDIRTSQALSWSVLTRCFIMPFINLTRLALLTICVLPIGL
                     AVTGMAQTEPNADAEKIRLSIDVSRPGAKIDRNIFGQFAEHLGHGVYEGIWVGPDSKI
                     PNTRGIRKDVVAALRALKVPNVRWPGGCFADEYHWRKGIGPQRVVTLNPNWGGVVEPN
                     TFGTHEFMDFLDQIGAEAYLSVNVGSGTPQEAAEWLEYLTTTQPTTLGKERAANGHPA
                     PYKIAYLGIGNESWDCGGNMTPDYYLSQLKIYSRFVRNFNPAQREEQQMLKIAVGPGG
                     REPRFAEWTEAVMKAWQNHQWSWDVNGLSLHNYTVVRWDNKLASVGFGETEYSQVLKS
                     TLDMEGLIAKHSAIMDKYDPQKKIALVVDEWGGWYAPLPGTDPGFLVQQNSLRDAVLA
                     ALNLNIFARHADRVRMSNIAQMINVLQAMILTDKEKMVLTPTYYVYKMYVPFQNATFL
                     PVAFNAGTYMHGDISLPRVDAIAAKDATGKLWFAITNLDPNQSIEIEATLAGSSGKSA
                     VGETLTAPKVDSVNTFEAPNTVVPRRVSTSVQGEKLTLKLEPKSVTVISVEQ"
     gene            3869..4765
                     /locus_tag="VFH91_03445"
     CDS             3869..4765
                     /locus_tag="VFH91_03445"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET6862067.1"
                     /translation="MTEPMDLGGDFTLAGTSMTVKRMGYGAMQLAGPEVFGPPRDVDG
                     AIAVLREAVAAGVNHIDTSDYYGPYVTNQIIKRALHPYPDDLVIVTKVGARRGDDKSW
                     IHALSRQELIDAVHDNLRNLALDKLDVVNLRVGGAMGPAEGSIEEPLTVLAELKHQGL
                     IRHLGLSNVSSKQLAESQTITDIVCIQNMYNVAHRHDDDFVDDLAKQGIAYVPFFPLG
                     GFTPLQSSRLEAAAASMDATPMQVALAWLLQHSANILLIPGTSSVEHLRENLKAATLR
                     LPPEIITDLDSIGRESSQGQHG"
     gene            complement(4900..5109)
                     /locus_tag="VFH91_03450"
     CDS             complement(4900..5109)
                     /locus_tag="VFH91_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6862068.1"
                     /translation="MLTISRDELRKKIVEGHQFLLVETLPEVAYHHGHLPGALNLPPD
                     KVKALAPTLLPDKNAEIVVYCASPT"
BASE COUNT         1351 a         1397 c         1474 g         1323 t
ORIGIN      
        1 gcggcaacag atgctgagat cgctcgcatg aaagaaacac tcgaatcagg tctcaaacgc
       61 ggggctgtag ctgtggggtt agggattcaa tacacgccac tggcatcaca ttgggagatt
      121 ttggagatgt ttcgtgttgc taagcgttac gacgcatcgg ttcacgtaca catgcgaaat
      181 gctggagtct tggaaccagc cagtagcgtt caagcgcttg aagaggtcat tgctgcggcc
      241 accattaccg gagcttcact tcatgttgtt cacataagca gtactggcgg tgcaaacacc
      301 ttgaagcttc ttcagatgat tgacgaggcc agggcgcgaa atctcgatgt tacaaccgag
      361 tgctatccct acctcgccgg aatgactgat atcaagtcgg cgatcttcaa tgaaggatgg
      421 cgggatgtgt ttggtattga ctacaaagat ctacagtggg cagcgaccgg agaacgcctt
      481 actgaagaga cctttgagcg ctatcgcaaa acgggtggga tggttgcgat gttttctatg
      541 acagaggaga tggtctccgg tgctgttaag agtcctctaa ccatgattgc aagtgacggt
      601 atcctggaaa acggtaaggg ccacccacga accgccggca cgtattccag ggttctaggt
      661 aaatacgtac gagagcaggg tgctctgaca ttaatggagg caatcactaa gatgactctt
      721 atgccggcgc aacgcttaga gaagcgcgta ccctcgatga aaaataaggg tcggattaaa
      781 gttggagctg acgcggattt cgcaatcttt gatcctaaaa cggttaacga tcgctcaacc
      841 tatcaagaac ctgcgcagta ttccgagggt ataaagtttg tcatcgtcaa tggcgagatg
      901 gtcgttaagg acggtactct tcagtcaggt gtttatccag gtcgtgcaat tcgagcccca
      961 ttttgaaccg gagctcgagc tggtttgtct aatgaggcct taccgcagat atttgttgaa
     1021 gaagtcgaga gtgcgggtcc aggccagttt ggcggcagcc tcgtcataac gcggagtcgt
     1081 atcgttatga aagccgtgct gtttgccatc gtacatgtat accgtataca ccttgttgtt
     1141 ggccttgagc gctgcttcgt aggccgcgat tccggcgttg acgccctggt cattgcctgc
     1201 gtaatggagc aatagcggcg cagaaatttt gggtacatcg tctgcgccgg cctgacggcc
     1261 ataaaatgca acgccggcat tcagatcagc acccaagcgc accgccaatt gattcactat
     1321 cccgccgccg aaacagaacc ctaccgcgcc cagtttgcct gtcgaatcgg gacgcgcttt
     1381 gagccactta gccgcggcga cgaaatcttc ggtcatcttc tttccatcga ctgtccgaaa
     1441 cgccgcggca cctttttcat cgtcgccggg ataacctccg accgaagtca atccatcggg
     1501 cgcaaaggcg atgaaattcg cgacagccag tcgacgcgct acgtcctcta tataaggatt
     1561 gagtccgcgg ttttcgtgaa tgacgagaac gacgggcagt tttccatttt ttgacggacg
     1621 cgccagatat cctttgatcg aaccgttccc agcgggtgat tccacgactt cgcggcccac
     1681 tttgatccgc ttgtcatcag gtggtacctg ctgtgcccag gcgtaattgg gtgtcaagct
     1741 ttcgaaaatt gtcgcgacgg ttagaccgcc gaccgcaaac ttgccggcgc gatcaaggaa
     1801 agtgcgacgg ttgatgtcgc cgtgttggta ttcgtggaaa aggtctaaca actcctgcgg
     1861 ataatcggat gctaatttac gttccatgtt ttcagcgctc ataacaatca cgtgggttgt
     1921 gtagtgtaaa cttcagacac cactcatttt gctacttggt cttgccagag tgtgttcgaa
     1981 cgtatcacat atttttgaag gagaacaaat ggaccgtaaa tcagatatcc ggaccagcca
     2041 agctttgtca tggtcggtac tcactcgttg tttcatcatg ccattcataa atctcacacg
     2101 actcgctttg ctaactattt gtgtacttcc tatcggtctt gctgtgactg ggatggccca
     2161 aacagaacct aatgctgacg ccgaaaagat tagattgtca attgatgtat ccaggcccgg
     2221 cgcgaagatc gaccgcaata tctttggcca gttcgcggaa catctcgggc acggcgtcta
     2281 cgagggcatc tgggttggtc cagattccaa aatccccaac acacgcggca tacgcaagga
     2341 cgttgtggct gcactcaggg cgctgaaagt cccaaacgtt cgctggccgg gcggctgttt
     2401 cgctgacgaa tatcactggc gtaaaggcat tggtccacag cgagtggtga cgctaaatcc
     2461 gaactgggga ggagtggttg agccgaacac tttcggaacc cacgagttca tggatttcct
     2521 ggaccagatt ggcgccgaag cttacctctc agtcaatgtc ggctccggta ctccgcaaga
     2581 agcagccgaa tggctcgagt acttgaccac cacgcagccg accacgctcg gtaaggagcg
     2641 cgccgctaac ggtcatcctg ccccatataa gattgcctac cttggcatcg gtaacgaaag
     2701 ctgggattgt ggtggcaaca tgacacccga ctactatcta agccagctca agatttacag
     2761 ccgcttcgtc aggaacttca atccggctca gcgagaagag cagcagatgc tgaagatcgc
     2821 tgttggtcca ggcggtcgtg aaccacggtt cgctgaatgg acggaagcgg tcatgaaagc
     2881 ctggcagaat catcagtgga gttgggatgt taatggactc tcgttgcaca actacacagt
     2941 cgtacggtgg gacaataagt tagcctccgt gggattcggt gagactgagt acagtcaggt
     3001 tctcaagtcc accctggaca tggaaggtct gatagctaaa cattcagcca tcatggacaa
     3061 gtacgatccg caaaagaaga tcgctcttgt cgttgacgaa tggggcggat ggtacgcgcc
     3121 gttacccggt accgatccag ggttcctggt ccaacagaat agtttgcgcg acgcggttct
     3181 ggctgcgctg aacctgaaca tctttgcgcg ccatgccgac cgtgtacgca tgtccaatat
     3241 cgcccagatg atcaatgtgc tgcaggctat gatcttgact gacaaagaaa agatggttct
     3301 gaccccaacc tactacgttt acaaaatgta tgtgccgttc cagaatgcga cctttttgcc
     3361 tgtcgcattc aatgccggca cctatatgca tggggacatc agcttgccgc gcgtggatgc
     3421 catcgccgca aaagatgcga ccggcaagct gtggttcgca ataaccaatc ttgatcccaa
     3481 tcaatcaatc gagatcgagg caacacttgc cggcagcagc ggtaagtctg cagtcggaga
     3541 aacgttaacc gcacctaagg tagacagcgt caacaccttt gaggcgccga acacagttgt
     3601 gcccaggcga gtttcgacaa gcgttcaagg cgaaaagcta actttaaaac tagaacctaa
     3661 gtctgtgacc gtgatctcag ttgagcaatg atgtgtacag gtcaaatgtt gtgtttttgt
     3721 ttgcgggtcg taggtcgctc attctgcgtt tataattctg atttactgat tcgaaatcac
     3781 tctcaagctc tggccaggtg aaggtcttcc acaaccggca cgatcgacaa gactgattaa
     3841 cgaatgcgac acacttagga gacacacaat gaccgaacca atggatcttg gcggcgactt
     3901 cacgctagcg ggcacttcga tgacagtgaa acgtatgggc tatggcgcaa tgcaacttgc
     3961 cggtccggag gtttttggac cacctcgtga tgttgacggc gcgattgctg tgctgcgaga
     4021 agcggttgcg gccggcgtca atcacatcga taccagcgat tattacggtc cttatgtgac
     4081 caatcagatc attaaacgag cacttcatcc ctatccagat gacctggtga tcgtaacaaa
     4141 ggtgggagct cgtcggggtg acgacaagtc ctggatccac gcgctttcac gtcaggagct
     4201 gatcgatgcc gtgcatgaca atcttcgaaa tctcgcgtta gacaagctcg acgtcgtcaa
     4261 tcttcgggtt gggggagcta tgggaccggc ggaaggttct attgaagaac cactgacagt
     4321 gctggcggaa ctcaaacatc aaggattgat tcgtcatctc ggtctgagta acgtcagttc
     4381 caaacagttg gctgagagtc agacgattac ggacatagtt tgcattcaga acatgtacaa
     4441 cgtagcacac cgtcacgatg atgactttgt ggatgacctc gcaaagcagg gaatagctta
     4501 tgtgccgttc tttccactgg gaggattcac tccgttacag tcgtcaagac tcgaggcagc
     4561 agcagcgtca atggacgcca ccccgatgca ggttgcgctc gcgtggcttc ttcagcactc
     4621 ggccaacatc ctgctgattc ccggcacatc ttcggtcgaa catcttcgtg agaatctcaa
     4681 ggctgcaacg ctacgacttc ctccagaaat aattactgac cttgattcaa tcggtagaga
     4741 gtcaagtcag gggcaacatg gttaagtagg gaactaatgt ttatgttcgc tttcgagcgg
     4801 cagcccagcc tcgatccagt cagacttgcc ctcggcgtaa tctcgaacgt tcgtgtagcc
     4861 cagcgccgcc aattcacggg cggcgttctc cgaagcatgt caagtagggc tggcgcagta
     4921 gactacgatc tccgcgttct tgtctggcaa gagtgttggt gccagggcct tcaccttatc
     4981 aggcggcaga ttcaaagcac cgggaagatg tccgtgatga taggccacct caggaagcgt
     5041 ttccaccagc aggaactggt gtccctcgac gatcttcttc ctcaattcgt ccctcgaaat
     5101 tgttagcact acaaacttct ccttttcccg aaggtttcaa taccacatta aagatgctcc
     5161 gtcctgttac cattgcacga taacaataaa caggaataag gttctggcaa gtataagaga
     5221 ccgagacccg aatacggaac cgcgagcgaa gcaccctcct tgatgtccga tatgctttag
     5281 cttgtcggtc ctttgaactt gagtgtgaat cgtgaccgac aagctaaagc ttatcggaca
     5341 cgaaggattg ggtcgcttcg ctcgccgttc ggtaatcggg tctcggtctc atatacttgc
     5401 cacaacgtta gtcctgttta ttgttatcgt gcaatggtaa caggacggag catctttaat
     5461 gtggtattga aaccttcggg aaaaggagaa gtttgtagtg ctaacaattt cgagggacga
     5521 attgaggaag aagatcgtcg aggga
//
LOCUS       DASSFP010000159         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_190504, whole genome shotgun sequence.
ACCESSION   DASSFP010000159 DASSFP010000000
VERSION     DASSFP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190504"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VFH91_03455"
     CDS             complement(<1..1101)
                     /locus_tag="VFH91_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862069.1"
                     /translation="MDSILIVDDERGIRDTLRAVMEDEGFVADAVATGEECLKALGKR
                     AYGCILLDVWLPGIDGLETLKQLRANGSDAAVVIISGHGNIETAVRATKLGAFDFIEK
                     PLSIEKTVLTVRNALRQQRLELINADMAAALAREYTMVGDSIAMRALRKQISVVAPTD
                     GRILIYGESGTGKELVARAIHLESRRGAAPFIEVNSAAIPEELIESELFGHVKGAFTG
                     ATAARMGKFELADGATIFLDEVGDMSQKVQAKVLRVLEEQRFEPVGSGNTINVDVRVI
                     AATNKRLDIEIEQGNFRADLFYRLNVIPFELPPLRERLEDVPQLLEYFNQLFSRAYGK
                     KPKQFMPEALEDLQSYSWPGNVREFRNTIERVV"
     gene            complement(1129..3393)
                     /locus_tag="VFH91_03460"
     CDS             complement(1129..3393)
                     /locus_tag="VFH91_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862070.1"
                     /translation="MLNSTPLTMTEGTENLSPEKLQRTRRRRRLLLWLLGATVAVLLS
                     IIVSQQLWLWTVVRPDTTSDALALYALSTLNFVAFVVFSFIFVRNLLKLRRERIERQL
                     GSKVKTRLLVYFISISFLPVTAMAVFSYLFLNRSLEKWFYPFPEIVQEASEVKRATLA
                     TQNRSLHDVASLLVMVLNQQDHDQRQNTIDQLISTGQLVGAEILQPDGRVTLKRQANL
                     PADDSEKLETLFAEARKTRESTGDTLADGKGFDVFAVPLNQSETLVIAPEKRAESDLN
                     ELISGSERGYQDLVGKQRQVRLLGVSTLGLMTLILLFVSSWVAIYLARGIATPIKALA
                     EASKEVARGNLSHRVTTVADDELALLAESFNQMTSQLEENRSSIEASAAELREKNLAL
                     RERRNYIETVLQSLSTGVVSVDENDCVTTLNPAASRMLGLTAKTDGNPKLSSLIGSDN
                     WIVADRLLRRARRAGHAAEQTQLETGSSNGSIPVAITATALRDASGPRRTVVLVLEDL
                     SELLSAQRAAAWSEVARRMAHEIKNPLTPIQLSAERIAKSYGRSNGGNGSNGKIDEPR
                     DEKRVANVIAECTETITREVAGLKAMVDEFSRFARLPPARREPASLNEVVAQAVGLYE
                     ERLDAVKIVMNLEQDLPVAMLDGEQLKRVFVNLIDNALDALTDANEKKEIVISTRHDT
                     DRSVLRVDVADTGLGIEEGDFRRLFQPYFSRRDSGTGLGLAIVQRIILEHGGRIRAER
                     NRPRGARFVIELPT"
BASE COUNT          869 a         1046 c          921 g          931 t
ORIGIN      
        1 caccacccgc tcaatcgtgt ttctaaactc acgcacattt cccggccacg aatacgattg
       61 aagatcctca agcgcctccg gcatgaactg ctttggcttt ttcccgtacg ccctggaaaa
      121 gagctgattg aaatattcca aaagctgcgg aacatcttct aacctttccc gcaacggcgg
      181 gagctcaaat ggaatcacgt tcagccggta gaacaaatcc gcacggaagt ttccctgctc
      241 gatttcaatg tccaatctct tgttagtcgc agcgataacc cgcacatcta cgttgattgt
      301 gttgccactt ccgactggct caaagcgttg ctcctccaat acgcgaagaa ccttcgcctg
      361 aactttctga ctcatgtcgc caacttcatc gagaaagatc gttgcaccat cagcaagttc
      421 gaactttccc attcgtgccg cagtcgcgcc tgtaaaagcc cctttcacgt gtccaaacag
      481 ttccgattcg atgagttctt cggggatcgc cgcagaattg acttcgatga acggcgctgc
      541 gccacgtctc gattcaagat gaattgcgcg cgccacgagc tcttttccag ttcctgactc
      601 tccataaatc aatatgcgac catcagtagg cgcgaccacc gagatctgtt ttcttaaggc
      661 gcgcatcgca atcgaatcgc ccaccatcgt gtactcgcgc gcgagggccg cagccatgtc
      721 agcattgata agttcaagtc gttgttggcg gagtgcattg cgaacggtga gtacagtttt
      781 ttcaattgac agtggcttct cgataaagtc gaaagcgccc aacttggtgg ctcgtactgc
      841 cgtttcaata ttgccatggc ccgaaatgat taccaccgcg gcatctgatc cattcgctcg
      901 caactgctta agtgtttcca gaccatcgat gcccggaagc cacacatcca gcagaatgca
      961 accataagca cgcttgccca gggctttgag acactcttct ccagtcgcta ccgcgtctgc
     1021 tacaaaacct tcatcttcca tgacggcgcg tagcgtgtcg cggatgccac gttcgtcgtc
     1081 gactatgagt attgaatcca ttaagtgtcg tagtaaccgg tctgtttctt aggtcggaag
     1141 ttcaatgacg aatctggcgc cgcgtggacg attgcgctcc gctcgaattc tgccaccgtg
     1201 ctccaggatg attctctgca caattgcgag acctaatccc gtgccgctat cacgtctgga
     1261 gaagtatggc tgaaacaatc gtcgaaaatc accttcctca attcccagtc ctgtatcggc
     1321 gacatcgact ctcaaaacac tcctgtcagt atcatgacga gtcgaaatta ctatttcctt
     1381 cttttcgttt gcatcggtca gcgcatcgag cgcgttgtcg atcaggttta caaaaacgcg
     1441 tttgagttgc tctccgtcga gcatcgccac cggcaaatcc tgctcgagat tcatcacgat
     1501 ctttacggcg tcaagtcgct cctcataaag accgactgcc tgggcaacta cttcattcag
     1561 gcttgcgggt tccctgcgag ccggaggcag gcgagcgaat cgggagaact catcaaccat
     1621 cgctttcaag cccgcgactt cgcgcgtaat tgtctccgta cattccgcaa ttacatttgc
     1681 gactcgcttt tcgtcgcgtg gctcgtcaat ctttccgttt gagccattgc caccgttgct
     1741 gcgaccgtaa ctcttagcta tccgttcggc ggacaactga atcggcgtaa gcgggttctt
     1801 gatctcgtgg gccattcttc tggcaacttc actccacgcc gctgctcttt gagcggagag
     1861 taattccgaa aggtcttcga gaaccaacac aacagttcgt cttggaccac tagcgtcgcg
     1921 caaagcggtc gcggtgatgg caacgggtat tgaaccattg ctcgagccgg tttcgagttg
     1981 ggtctgctcg gcggcgtgtc ctgcgcgacg ggcccggcga agcagcctgt cggcgacaat
     2041 ccagttgtcg ctacctatta agctacttag cttcgggttg ccgtcagtct ttgcagtcaa
     2101 tccgagcatt cgggaagcgg cgggatttaa agtggtcacg caatcgttct cgtccactga
     2161 cacgacgcca gtcgatagag actgcagaac agtctctatg taattacgac gttctcgaag
     2221 tgcaaggttc ttctcgcgta actctgccgc gcttgcttca atactactgc ggttctcctc
     2281 taactggctc gtcatctgat tgaatgactc ggccaataaa gctagctcat cgtcagctac
     2341 cgtcgtgact cgatgtgaaa gattgccccg cgctacttcc ttcgaagctt cagcgagagc
     2401 cttgatagga gttgctattc cccgcgccag gtagatcgct acccaactcg atacgaacaa
     2461 cagaatcaat gtcatcaagc ctagcgtcga gacaccgagc aatcgaacct gtctctgttt
     2521 tccaacgaga tcctgatagc ctctctctga gccggaaatg agttcgttca aatcactctc
     2581 ggcccgtttc tctggcgcaa ttacaagcgt ctcagattga ttcaaaggca cggcaaaaac
     2641 gtcgaaccct tttccgtctg caagagtatc tcccgttgat tcacgcgttt ttcgcgcctc
     2701 tgcaaacaat gtctcgagct tttcggaatc gtcagccggc aagttcgcct gtcttttaag
     2761 agttacgcgg ccatccggct gtagaatttc cgctcccacc agttggcccg ttgagatcaa
     2821 ctgatcgatt gtgttttgac gttgatcatg gtcctgttga ttcaacacca ttacgagtaa
     2881 cgacgcgaca tcgtgcaagg agcgattttg agtagccagg gtggctcgtt tcacttcgct
     2941 ggcttcctgg acaatttccg gaaagggata gaaccatttt tcaagcgagc gattgaggaa
     3001 caagtacgag aataccgcca tcgcggtaac aggaaggaag ctaatggaga tgaaatagac
     3061 caggagtctg gtttttacct ttgagccgag ttgtcgctcg atacgctcgc gccgcaactt
     3121 gagtagattg cgaacaaaga taaacgagaa gactacgaac gcgacgaagt tgagggttga
     3181 aagagcgtaa agtgcgagcg catcagacgt tgtgtcgggt cgcacgacag tccacagcca
     3241 aagctgttgg gagacaataa tcgacagcaa tacggcgact gtggcgccca aaagccacaa
     3301 gagaaggcgg cggcgtctac gagtgcgctg cagtttttcc ggggatagat tttcggtgcc
     3361 ttcagtcatg gtaagtggtg tcgagtttaa catagcgacc acttctcagc tgtgagccac
     3421 caccggtaca ggtggagtaa tctttggagc gaacctaaaa ctcaccgaac aaaccaatac
     3481 agtcagcttc tgcgccctct gcgtctctct gcgatacctc tgcgtttctc tgcgatcctt
     3541 cgactcagcc aaaatcggtc tcccgttcag cgcatcgcag agagacgcag aggtctcgca
     3601 gagggacgca gagaagagat tttccagaat tatgcagtta gcggggctga ttggtgtata
     3661 cctcgagcat taagctagaa tgccccggcc caaattattc agatgacctt cagcaaaatc
     3721 ttcattaacg acgtaggtcg actgcggagt ggctggcgcg cttcgat
//
LOCUS       DASSFP010000160         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_778218, whole genome shotgun sequence.
ACCESSION   DASSFP010000160 DASSFP010000000
VERSION     DASSFP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778218"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFH91_03465"
     CDS             complement(<1..465)
                     /locus_tag="VFH91_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862071.1"
                     /translation="MRIELVPPDPDTEPDYWVPQDQALAMMLRPGSMECGSKRSALER
                     FDYGPGDLALCHRHEGEWFGLMNVPHLQLGISDAALLASSDAAYGEVELRPSRKFADP
                     RLSALVAAVDAEMVAGFPSGRLFLDSVEQAIAVALVNGHAVRHRPVQIYRGGL"
     gene            1173..1556
                     /locus_tag="VFH91_03470"
     CDS             1173..1556
                     /locus_tag="VFH91_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6862072.1"
                     /translation="MLKVISSRTGNVVALRIQGRIVRGETDRLRTAVVSQPDASVIVL
                     DLARVHTVDAGGLGLMLELREYAESRGVEFKLRNVTNLVRRIIELTRLDSVLDVTYAS
                     ESAARSSFVRFGSQMQSSCCGYART"
     gene            1686..2168
                     /locus_tag="VFH91_03475"
     CDS             1686..2168
                     /locus_tag="VFH91_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6862073.1"
                     /translation="MDTDFANIAGMYKTNTDIVGKTISEVSDERWFDKPGDDSNHLMW
                     VVGHLVWSRWNVLKLLGQEWDIPAARLFERGATVANQDEYPTVEAMKTAWAGVSGKLA
                     ATLADASDETLSKSAPKGPPTFDGKLSGVVAFLAFHETYHVGQVSYLKKWLGYGQAVG
                     "
     gene            2245..2586
                     /locus_tag="VFH91_03480"
     CDS             2245..2586
                     /locus_tag="VFH91_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6862074.1"
                     /translation="MSEFVFLYRGGEAGRSPERYQQTLQKWMAWFREMAAKGHVKNQG
                     HPLERAGSIVKGSAKSVIDGPFVEAKDVIGGYSLIEAKDLEEAIELSKGCPILDAEGA
                     VEVRPVMQLNI"
     gene            2583..>2884
                     /locus_tag="VFH91_03485"
     CDS             2583..>2884
                     /locus_tag="VFH91_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET6862075.1"
                     /translation="MTEIDGLGDHLFRHEAGRMIATLTRIFGVHNLALAEDVVQDAFC
                     RALEVWKFRGVPENPSAWLMVTAKNRALDVLRRERTARTFAPELTRLLESEWTLAPT"
BASE COUNT          723 a          699 c          800 g          662 t
ORIGIN      
        1 tagtccgcct cggtatatct gcacaggacg gtgccttact gcatgaccat tgaccagcgc
       61 tacggcgatc gcctgttcga cagaatccag aaacagccgc ccgctcggaa agccagcaac
      121 catctccgca tcaacggccg caaccagcgc actcaggcgt gggtccgcaa acttgcgtga
      181 agggcgcagt tctacctcac cgtaagctgc gtcagagctt gccagcagtg ccgcgtcgga
      241 aatgccgagc tgcagatgcg gcacgttcat gagtccaaac cactcaccct catgacgatg
      301 gcacagtgct aagtcgcccg gcccatagtc gaacctttcc agcgcggaac gcttcgagcc
      361 acattccatc gaaccaggcc gcaacatcat tgctaaggct tgatcttgag gtacccaata
      421 atccggctct gtgtcagggt cgggcggaac gagttcaatt ctcattctct tactccatgc
      481 atcgtgtcgt aaacagtatc acgcgtcccc gaggcgattc aattagactt tggtggagct
      541 agatcgcaac gagcttgagt ggaggcctcc aacactaatt taggtttgtg gtgtttgaca
      601 cgatttgcga caaggcgatc gtcaactaac agatggcgga ggcagattca tattccgtcg
      661 agtagcgact ggttatgctt cttcggtaga agtccagggt gaatcgactg ctggcgtgtc
      721 gcattagttc ctggaccacc ttcacattct caccattcgc cgtcgagcac tacctcgaca
      781 cggcaggggg ctggggttca aatccccacg cgcctaccaa tagaaacagt aagttacgat
      841 cataggactc ttcggagtcc tttttttatg acgcgagtta tgacgtaatc tttggtgctg
      901 ggcgattcaa cccagaacgg tttccatcct tggaaaaata gtctgaaact tcttgttcat
      961 cactttggat ttcgtcgtgc agcaaaaagg ttcaggcttt cagacattgt acatactgag
     1021 gacaaaggtc tggaaataat tgtccggccg aagatgctcc aaccgtctta atgatggaat
     1081 cattctggtg atcgaaagtt taaggattgt cgaattgaat gattcccgtt cgactaactt
     1141 gtgaaacaat ctggtagtaa aaggtggaac ttatgttgaa agtcattagt agcagaacgg
     1201 gaaacgtcgt ggccctccgt attcaaggtc gaattgtacg tggtgagact gacaggctac
     1261 gaacagctgt tgtttcacaa cctgatgcga gtgtgatcgt actggacctt gcacgagttc
     1321 acaccgtcga tgcgggtggc ttgggtctaa tgctggaact gcgtgaatat gctgagtcga
     1381 gaggtgtcga gttcaagctg agaaacgtta cgaacctcgt acgaagaatc atcgaactca
     1441 cgaggctcga ttccgttctc gatgttacat atgcatcaga gagcgcagct aggtcgtcat
     1501 tcgtacggtt cggttcgcaa atgcaaagta gttgctgcgg gtacgcgaga acatgagccg
     1561 agactgtagg gacgtccctc cctcggtact ctttcacggg gaccaacatt acacccaaag
     1621 gtcgggctaa atacaaaggg gcggcacgga ggagggccac gcctaaaata gaactcggag
     1681 gagtcatgga tactgatttc gcaaacatcg cgggcatgta caaaacgaac acagacattg
     1741 tcggcaaaac tatctcggaa gtttcggatg aacgttggtt cgataagcct ggcgacgatt
     1801 caaatcactt gatgtgggtg gtcgggcatt tggtatggag tcgatggaac gttctcaagc
     1861 tactcggtca agaatgggac attcctgccg cccgactatt cgaaagaggc gcgacggttg
     1921 ccaatcaaga tgaataccca actgtcgaag cgatgaagac agcctgggcc ggggtttcag
     1981 gaaaactcgc tgcgacgctg gctgatgcat cagatgagac actttcaaag tcagcaccca
     2041 agggaccacc gaccttcgat ggcaaattaa gtggtgtcgt cgcattcctt gcctttcacg
     2101 aaacctatca tgtaggccag gtaagttatc tcaaaaagtg gcttggctac gggcaggccg
     2161 ttgggtagaa agaaatgtgt ctgctgaggc gcgggtcaca cgtcttagag ttgcagtacg
     2221 aactaattgc aacggaggat tgagatgagc gaatttgttt ttctttatcg tggtggtgag
     2281 gcaggcagat caccggagcg gtatcaacag acattgcaaa agtggatggc ctggttcagg
     2341 gaaatggctg caaaggggca cgtcaagaat caagggcatc cgctcgaacg cgcaggcagt
     2401 attgtgaaag gttctgcgaa gtcagtcatt gatgggccgt ttgtcgaagc taaggatgtt
     2461 atcggcggat actcattgat cgaagcgaag gatcttgaag aggcgatcga gctatccaaa
     2521 ggatgcccga ttctcgacgc ggaaggggcg gtagaagtac gtccagtaat gcaactcaac
     2581 atatgaccga aattgatggg ctaggcgatc atctgtttcg ccatgaagct ggccgcatga
     2641 tcgcaacgct cacgcgaatc ttcggtgttc acaatttggc gttggcagaa gacgtcgtgc
     2701 aagacgcctt ctgccgcgca cttgaagtgt ggaagtttcg cggcgtgcct gagaacccat
     2761 ctgcgtggtt gatggtcacg gccaagaacc gtgcactcga tgtactgcgg cgcgagcgaa
     2821 cagcgcggac atttgcgccg gaactaactc gcttgctgga aagcgagtgg acactcgctc
     2881 caac
//
LOCUS       DASSFP010000161         6503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1677311, whole genome shotgun sequence.
ACCESSION   DASSFP010000161 DASSFP010000000
VERSION     DASSFP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677311"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFH91_03490"
     CDS             <1..387
                     /locus_tag="VFH91_03490"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET6862076.1"
                     /translation="DTDNNPKTVADLNWLPQAGKTAPDPSYPGAHAVISAAGAEVLIS
                     FFGRDHFKFSVTSEVLPGVERSFTNFSVAAEEATLSRVFAGQHFSFDLTSGQRLGREV
                     ADFVVENFLRPGRGDTAQKGRNQRKM"
     gene            594..3290
                     /locus_tag="VFH91_03495"
     CDS             594..3290
                     /locus_tag="VFH91_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6862077.1"
                     /translation="MDTLINNLTAIVGRDAVLSEPDQLLVYECDGLPHHKYRPRAVVF
                     PNSTEQTSAVMRELARAGVPFTPRGAGTGLSGGALALNSGVVIEMARMRKILRIDPDN
                     RIAVVQPGVVNLHVSRAVAPYGMYYVPDPSSQPTCTIGGNIAESAGGIHCLKYGTTTD
                     HVLGCKVVLSGGEVVELGGFGVEDYGYDLLGTFIGSEGTFGIATEATLRLVQIPPAIR
                     TLLAEFGEVNDASHAVSAIIAAGVMPAALEMMDKEIIRAVEASVFAAGLPPDAGAALL
                     IELDGIEAGLDLEADRVINICLEFGARSCRLARDENERKKLWAARKGAFGAIGRISPD
                     SMIQDAVVPRSRLPEVLGAAYEIAARNKLRIANVFHAGDGNLHPLICFDSRFPEEVLR
                     VKEAGRELMQVCVNAGGTITGEHGVGLDKRELLPLVFSDADMEAMLSVRAAFDPLGVC
                     NPGKVIPMLRGCGEGKAIVTTELAAEVKLDVLVSAAPKRSIQPRTGENIAHQLASLVG
                     VDHVRLDSSQLVVSPSSEGEIRDIISLASREKFVVVPCGGGTWLPNVKQEHVFLNTQR
                     LNRVIEHEPADLVATAESGVRLLALNETLAENGQWLPLDPPDDDTATLGGVVACGIGG
                     SQQFGYGKPRGSVIGMKVALANGELIKAGGRVVKNVAGYDLCKLFTGSFGSLGVITEL
                     TFKLRPRPAVEQTIVAVGELSRLYYAARSIIDNRLFPVAVELISPALASELRSMPEGE
                     NSVLLVRFAGNHGAVKFQEEKALSLIESNCSSVDVVVDDAELWRQIAMLPFNQKNEWR
                     ANVLPASLPVFLESLSDLIGNDFDNWKWQMNVGEGRVRVFDELQRDQQQVDQLTVLTN
                     SLDGHLVMAHSQFQGKKSSQQLAERIKTKLDPNGVFPQPSFR"
     gene            3324..5066
                     /gene="ggt"
                     /locus_tag="VFH91_03500"
     CDS             3324..5066
                     /gene="ggt"
                     /locus_tag="VFH91_03500"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET6862078.1"
                     /translation="MKTKSSIALTVVLSILLTISWVVEFQSPTAFAGGRQPVRARNGM
                     VASTNEVASRVGVDIMKRGGNAVDAAIAVAFALAVTHPAAGNLGGGGFMMIRLKNGTT
                     TAIDYREMAPASAHRNVFLNKNGDLIEGEGGSLVGYRAAGVPGTVRGMELALKKYGSG
                     KLNWAQLIEPARRLAVEGFVVTHELANSLYGNRKYLSQYAETRRIYLKNGQFYQESER
                     FQQPELGATFTRLQKLGPDEFYQGETAKLIVADMKRNNGLMTMDDLRGYVAKERTPLR
                     GTYRGYEIISMPPPSSGGAVLIEMLNILEGFEIDKLDANSSDRYHLMAEAMRRAFADR
                     AEYMGDSDFVKVPVSGLTDKAYAAQLRSSISPERASNSKDVRAGRPTGYESDETTHFT
                     VVDAQGNTVANTYTLNNSYGSAAVAKGTGIILNDEMDDFAAKPGTPNMYGLIQGERNA
                     VAPGKRPLSAMTPTIVLRKDGSLWFTIGSPGGPTIINTVLCVISNVIDYDMNIQEAID
                     APRIHHQWLPDELVGEPFGLSGDTQRALTARGHTLAKLRYLGDAEGIMIEEKTGMRLG
                     ATDPRRSDGLAVGY"
     gene            5116..6417
                     /locus_tag="VFH91_03505"
     CDS             5116..6417
                     /locus_tag="VFH91_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6862079.1"
                     /translation="MSLESFANQEQKLLACIHCGLCLEACPTYVVTGNENDGPRGRLY
                     LMRSVAEGRLPNTSTAFSEHIDRCLGCRACEQVCPAGVEYGVLLEASRQELLAAQPKA
                     DLQNRLLRFVLRHVWLSPARLRIMFAGARLFRDLRLTSLLLKTGMARLISRQAEFGLT
                     LLDSSRSSRPEPADTRLQNGKPSEVMSLFRGCVGEGLFGRVNRATRRVLEANSVSVNS
                     PENQVCCGALHAHAGDLEGARILARQNIKAFAGDDVIITNAGGCGAMLVSYAHLLSED
                     EDYRDTAQEFSERVKDVSQELQRRQIRTGAPATDERVTYDTSCHLLYGQHAGESSQKM
                     VQQIPGLDFVALQGTERCCGGAGIYNLMQPEMSSRVLREKLDCIKDTGAELLATGNPG
                     CQMQIAAGACLEGKKLKVCHPIELLDESYARAGFYDEENGG"
BASE COUNT         1569 a         1558 c         1834 g         1542 t
ORIGIN      
        1 gatacggaca acaacccgaa aacagtggct gatctgaatt ggcttcccca ggctggaaag
       61 actgcgcctg acccatcata tccaggtgct catgccgtca tcagtgctgc aggggcggag
      121 gtattgattt ccttcttcgg cagggaccat ttcaagttca gtgtgacctc tgaggttctc
      181 ccaggcgtag aacgctcgtt cacgaacttc tctgtcgcag ccgaagaagc gacgttgagc
      241 cgtgtcttcg cgggacagca tttcagcttt gaccttacca gcggtcaacg tctcggtcgt
      301 gaagtagctg actttgttgt cgagaatttc ttaaggcccg gtcgcggaga cacagcgcag
      361 aagggccgca accaaagaaa gatgtgactg gaacaggtca gtaccgattc cggagatgtc
      421 cgatatgctt cagcttgtcg gtttgactcg ttcctctgaa cttgagtgtg catcatgacc
      481 gacaagctga agcatatcgg acatctccgg aatcggtact gacctgctcg agacccaaag
      541 gaaagcacga aaaaccttgt aatggagtaa actatagccc aagaaactcc agcgtggaca
      601 cattaatcaa caacctaacc gctatcgtgg gacgggacgc tgttctgtcc gagcctgatc
      661 aacttctcgt ctacgaatgt gatggattac cgcaccataa gtatcgacct cgcgcagtcg
      721 tgttccccaa ctcaaccgaa caaacctcag ctgtaatgcg tgagctggct cgagcaggcg
      781 tcccgttcac ccccagagga gctggtaccg gattatcagg aggagcactc gctctcaata
      841 gcggcgtcgt gattgaaatg gcccgcatga gaaagattct gcggatcgat ccagacaatc
      901 gaattgcagt agtgcaaccg ggtgttgtga accttcacgt atcgcgggcc gtcgctcctt
      961 acggtatgta ctacgtaccg gatccttcca gtcaacctac gtgtacgatt ggaggcaata
     1021 tcgcagagag cgcgggtggc atccactgtt tgaaatacgg cactaccact gatcacgtac
     1081 ttggctgcaa agtggttctc agtggaggcg aagttgtcga acttggtgga tttggagttg
     1141 aagactacgg ttacgatctg ctcggcactt tcattggctc agaaggaact tttggcatcg
     1201 caactgaggc aaccttgcgc ctggtccaaa tcccaccagc cattcgaaca ttgttggcag
     1261 agttcggtga agtcaacgat gcgagtcatg cagtctcagc aatcattgcg gcgggtgtaa
     1321 tgccggctgc acttgagatg atggacaaag agataatccg cgccgtcgaa gcaagcgtgt
     1381 ttgctgctgg tttaccgcca gatgctggtg ctgcccttct gatcgaacta gatggaatcg
     1441 aagccggttt agatctcgag gcagatcgag ttattaacat ctgtctcgag tttggagcgc
     1501 gaagttgccg cctggctcgc gatgaaaatg aaaggaagaa gctctgggct gctcggaagg
     1561 gtgctttcgg tgctattggt cgcatttccc cagattcgat gatccaggat gcagttgttc
     1621 ctcgttctcg tctcccggag gttcttggcg cagcgtatga gattgcggcg agaaacaaat
     1681 tgcgaatcgc caatgttttc catgccggcg acgggaatct acatccactc atctgtttcg
     1741 actcgaggtt tccggaagaa gtgctccgcg tgaaagaggc cggccgagaa ctaatgcagg
     1801 tgtgcgtgaa cgcgggcggc acgattacgg gagaacatgg agtcggtctc gataaacgcg
     1861 agttactgcc gctcgtgttt tccgacgctg acatggaagc gatgttgagt gtccgtgcgg
     1921 cgtttgatcc actcggcgtc tgcaacccag ggaaagtgat ccctatgctg cgaggttgcg
     1981 gagaaggtaa agccatcgtc acaactgaac ttgcagctga agtcaaactg gatgtacttg
     2041 tctccgccgc tcccaaaaga tcgatccaac ctcgcaccgg tgaaaatata gctcatcagc
     2101 tggcatcact ggttggtgtc gatcacgtca gactcgattc gtctcagctt gtcgtttcgc
     2161 cttcctcgga gggtgagatt agagacatta ttagcttagc ttcacgcgaa aagttcgtgg
     2221 tggttccttg tgggggcggc acatggctgc caaacgtgaa acaagagcac gtctttttaa
     2281 atacccaacg actcaataga gtaatcgagc acgaacctgc ggatcttgta gcaactgcgg
     2341 aatcaggtgt gcgtctgctg gcattgaacg aaacactggc tgaaaacggt caatggttgc
     2401 cactcgatcc acccgatgac gacacggcaa cgcttggagg cgtcgtcgcc tgtggaatcg
     2461 gaggatcgca gcagtttggt tatggaaagc cgcgcggatc cgtgattggg atgaaagtgg
     2521 ctctcgcgaa tggtgagtta atcaaagcag gtggacgagt cgtaaagaat gtcgctggct
     2581 acgatctctg caaactcttt acaggaagct tcgggagcct tggcgtcatc acggagttga
     2641 ctttcaagtt gagaccgcgg ccggccgtcg aacagaccat tgtcgcagtt ggcgaacttt
     2701 ctcgtctcta ttacgccgct agatcgatca tcgacaatcg tttgtttcca gtggctgttg
     2761 aattgatctc gcccgcgctt gcgagtgaat tgcggtcgat gccagagggc gaaaattcgg
     2821 tcttactagt ccgctttgcc ggcaaccacg gggccgtaaa atttcaggag gaaaaagctc
     2881 tatcattaat tgagtccaat tgctcctctg tcgatgtagt cgttgacgat gctgagctgt
     2941 ggcggcagat cgcgatgttg cccttcaatc aaaagaacga gtggcgggca aacgttctac
     3001 cggcatcgtt accggtgttt ctcgagtcgt tgagcgacct gattgggaac gattttgaca
     3061 attggaagtg gcagatgaac gtgggagaag gtcgtgtccg agtgtttgat gaattgcaaa
     3121 gagaccaaca gcaagtggac cagttaaccg tactgaccaa ttctctagac ggtcatttag
     3181 taatggccca ttcacaattt cagggcaaga aatcttcgca gcaacttgcg gagcggataa
     3241 aaacaaaatt ggatccaaat ggcgttttcc cacaaccctc atttcgatga tggaaggatc
     3301 gaaaagtcac catgaagaca aagatgaaaa cgaaatcgtc gatcgcctta accgtcgttc
     3361 tttccatcct actcacgatc tcgtgggtag ttgagtttca gagtccaact gcttttgcag
     3421 gtggccgcca acccgtacgt gcgcgaaacg gaatggtggc gtcgacaaat gaagtcgcct
     3481 ctcgcgtcgg agtggacatc atgaagcgcg gtggcaacgc tgtggatgcg gcaatagcag
     3541 tcgccttcgc tctagcggtt acgcatccag ctgccggcaa tctcggaggc ggcggattca
     3601 tgatgatccg acttaagaat gggaccacaa cagcgatcga ttatcgcgag atggctccgg
     3661 cgtcagcgca tcgaaatgtt ttcctgaata agaatggaga cttgattgaa ggagaaggtg
     3721 gttcgttagt tggatatcga gcggcgggcg ttccgggaac tgttagagga atggaactgg
     3781 cccttaaaaa gtacggctcc ggaaaattga actgggctca actgattgaa cccgctcggc
     3841 gacttgctgt tgaagggttt gttgttactc acgagctggc caattccttg tacggaaatc
     3901 gtaagtatct ctcccagtac gccgagacca ggcgcatcta cttaaagaat ggacagttct
     3961 atcaggagag cgaacgcttt cagcaaccag agttaggtgc gacattcaca cgcttacaaa
     4021 agctcgggcc tgatgagttt taccagggtg agactgcaaa actcatagtt gctgacatga
     4081 aacgcaacaa tggcttgatg acgatggatg acctgcgtgg ctatgtcgca aaggaaagga
     4141 ctcctttgcg tggtacgtat cgtgggtacg aaataatttc gatgccacca ccgtcttcgg
     4201 gcggcgctgt attgatcgag atgctcaaca tccttgaagg ctttgagatt gataagctcg
     4261 acgccaactc ttccgatcga tatcacttga tggcagaggc aatgcgacgc gctttcgcgg
     4321 accgtgctga gtacatgggc gacagcgatt ttgtcaaagt tcccgtgtca gggctgacgg
     4381 acaaagcgta cgcagctcaa ctgcgatcca gcataagtcc tgagcgagct tcgaacagca
     4441 aagatgttcg cgccgggcgg ccgacggggt acgagtcgga tgagacgacg cactttacag
     4501 tcgtggatgc gcagggcaat accgttgcaa atacttacac cttgaacaac tcctacggtt
     4561 ccgcggcagt agctaaaggg acgggaataa ttctgaatga tgagatggat gattttgctg
     4621 caaagcctgg tacgccaaat atgtacgggt taatccaggg cgagcggaat gcagtcgcgc
     4681 ccggcaagcg ccctttgtcg gcaatgactc cgaccatcgt cttacgaaaa gatggaagtc
     4741 tctggtttac gattggtagt cccggtggcc caacaataat caatacagtg ctttgtgtaa
     4801 tctcgaatgt gatcgactat gacatgaata ttcaggaggc cattgacgct cctcgtattc
     4861 atcatcagtg gcttccagat gaactggtgg gtgagccatt cgggttatct ggcgatacgc
     4921 aacgagcgtt gacggctcgg ggacatacat tggcaaagct tcgatatttg ggcgacgccg
     4981 aaggaatcat gattgaggag aagacaggga tgcgtttagg ggctacggat ccgcggcgaa
     5041 gtgatgggtt ggcggtgggg tattgaaaaa ggagatagga cctatttgac ctataggacc
     5101 tatcggggaa tttgattgag cctcgagagt ttcgcaaatc aggaacaaaa actccttgct
     5161 tgcattcact gcgggctctg cctcgaggcg tgcccaacct acgtcgtcac cggcaacgag
     5221 aatgatggac cacgcggacg cttgtacttg atgcggtcag tcgctgaggg tcgacttccc
     5281 aatacttcaa ccgcctttag cgaacacatc gatcgttgcc ttggctgtcg tgcctgtgaa
     5341 caggtctgtc cggccggcgt cgaatatgga gtgctattag aagctagtcg acaggaattg
     5401 cttgcggctc aaccgaaagc cgatcttcag aaccggttat tgcgttttgt cctcaggcat
     5461 gtctggttat caccagcacg tctgcggata atgttcgccg gtgcgcgtct attccgggat
     5521 cttcggctaa caagcttact tctgaaaaca gggatggcgc gactcatatc acgccaggct
     5581 gaattcggat taactttgct ggatagttcg cgctcgagtc gtccggaacc ggctgacact
     5641 cgcttgcaaa atggtaagcc gagcgaagtt atgtcgctct ttcgaggttg cgtaggcgag
     5701 ggactttttg gtcgggtgaa ccgcgcaacc aggcgcgtac ttgaggccaa ttctgtttcg
     5761 gtaaactctc ctgagaacca ggtttgttgc ggagctttgc atgcacacgc gggtgatctt
     5821 gaaggagcgc gaatactagc acgtcaaaat atcaaagcgt tcgctggcga cgacgtgatc
     5881 atcaccaatg caggcggttg cggtgcgatg ttggtttcct acgcccactt gttgtcggaa
     5941 gatgaagatt atcgagatac ggcccaggag tttagtgaaa gagttaaaga cgttagccag
     6001 gaactgcaac gacgacagat tagaaccgga gcgccggcaa ccgatgagcg agttacttac
     6061 gacacttcat gtcacttgct ctatggacaa cacgcgggtg agtcatcgca gaagatggtc
     6121 cagcagattc caggtctcga tttcgtcgct cttcagggca ccgaacgttg ctgtggcggc
     6181 gctgggattt ataacttgat gcaaccagaa atgtcctctc gtgttttgag agaaaagttg
     6241 gattgcataa aagatactgg cgctgaactt ctggcgaccg gcaatcctgg ttgtcagatg
     6301 caaattgcgg cgggagcttg tttggaggga aagaagttaa aggtatgtca cccaattgaa
     6361 ctcctcgacg aatcctacgc ccgcgccggc ttttatgatg aggagaatgg tgggtgagtt
     6421 aaacttttcg tcatggcaag ctaatagcac aggtcagtac cgattccgga gatgtccgat
     6481 atgcttcagc ttgtcggttt gac
//
LOCUS       DASSFP010000162         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_35806, whole genome shotgun sequence.
ACCESSION   DASSFP010000162 DASSFP010000000
VERSION     DASSFP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35806"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFH91_03510"
     CDS             complement(<1..219)
                     /locus_tag="VFH91_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862080.1"
                     /translation="MTERSIFIRGGESRFMIYCKNSNTTHVSGWMVQILSTTSYKGFG
                     NTTHVSGWMVQILSTTSYKGFGNTTHVSG"
     gene            complement(238..1161)
                     /locus_tag="VFH91_03515"
     CDS             complement(238..1161)
                     /locus_tag="VFH91_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET6862081.1"
                     /translation="MKLLDRSSLKKLRKDEVRELGRIYRRTASDLAIARAESRDPRLV
                     NYLNSLVIRAHGRIYRADAQGGKRIRNYFARELPRTFRRTWRYTAFSFAIFASFAVFS
                     FVATRYDPEFSELVGVNPAFRESNIETRTPWWEDLNDKNQIGAAFIMQNNIRVTIYTF
                     AFGAMLGLGTVYYLGFNGANMGAVLALTYRAGFGNDLLTFMVGHGVIELSCIFIAGGA
                     GLLIGSAMLMPGDLSRADALKARGAEAVRLMLGVAVFLVIAGTIEGFVSPAPIDPKIK
                     YGIAILTGIALYSYLLLAGRDSTPVEETLMK"
     gene            1275..2192
                     /gene="ftcD"
                     /locus_tag="VFH91_03520"
     CDS             1275..2192
                     /gene="ftcD"
                     /locus_tag="VFH91_03520"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET6862082.1"
                     /translation="MTGLIECVPNFSEGRDAAVVEMIRSAILRVPTSCVLDTHIDADH
                     NRSVITFVAHADHVVEAAFSAVKQAAELIDLRKHIGEHPRLGATDVLPFVPIKNATME
                     DCVRLAHEAGKRIAAELAIPVYFYERAALREDRVNLEDVRRGAFELLRDQITTVTSRI
                     PDEGAARIHESAGAIIIGARPFLIAFNCVLASDDLAAAKQIAKAIRARSGGLPFLKAL
                     GFKLASRNRVQVSMNLLNYRVTGMREAYEAVRREAEKVGIEVESAEIVGLVPQDALNE
                     SEEYFQKLDESSRLKILENQLARCGPFEA"
     gene            complement(2458..4155)
                     /locus_tag="VFH91_03525"
     CDS             complement(2458..4155)
                     /locus_tag="VFH91_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862083.1"
                     /translation="MPRSPNERLVEQLSLLKSDFSVAAARSIGTLLGQLRQLTFTDPE
                     TLVLYHETLLFLRAYPHDGPTVRAVEAQLRTFDQRIKFLREHDADLSPIMHPEISGIF
                     GDSVSDTFSFDIVRWLVRKYPEQIQVDWDWFEDENRLADAWPRFMPLLEEDSFVEANI
                     PYREWLRQARNGTSEVEWLLSRFDKLPLTDKEKAEIYNAQQLYVRWTPGHRSTRTGLR
                     STNNQPIFFQRSPLIQRRDVILRHELSKPVSTSRLLSLTEGERALDLARDASTVRYRE
                     LYGFTHGDPKRVFDVRLDRGLCLHVIGLPAERRLPLRTYHAAMIYRNAVPIGYFEGLS
                     LCEKMESGFNLYYTFREGETAWIYAQTLNVMRQIAGVTTFSLDPYQIGYENEEGIESG
                     AFWFYRKLGFRSTDPAVRKLTAKEEKKLSTKKNYRSSAATLRELARAPMIFELDANRQ
                     EDWDRFQVRNIGMRVQKRMAQAFNGDASLMRKKTLNTVAGALNVESSQLNKSQQQAFS
                     AFALVLSLVRDLREWEEHEKRQLISIIRAKAGVDETRYLRETQKHQRLRRELIRLGS"
     gene            complement(4177..>4851)
                     /locus_tag="VFH91_03530"
     CDS             complement(4177..>4851)
                     /locus_tag="VFH91_03530"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET6862084.1"
                     /translation="TPPQVWMVHSGGFYVVERRKVPDLVSRTLHWFRWEAATTWLSGL
                     ALLILIFYLGGGALVDVDVRDLSVGVAVLIGIGVLVGGWIVYDVMMLSPLGGNERVFA
                     VIAYILIVALCYALTHIFSGRAAYIHVGAVFGTIMAANVWQRILPAQRKMIEALKNGQ
                     RPDEKLASQAKLRSKQNTFMAVPVVLIMISNHYPGVTYGERNNWLVLSGLILVGWISA
                     KFIRRA"
BASE COUNT         1240 a         1229 c         1223 g         1159 t
ORIGIN      
        1 cccactcacg tgtgtggtat ttccaaaacc tttgtagcta gttgtagaaa ggatctgaac
       61 catccaccca ctcacgtggg tggtatttcc aaaacccttg tagctagttg tagaaaggat
      121 ctgaaccatc cacccactca cgtgggtggt atttgagttt ttacagtaaa tcatgaatct
      181 cgattcacca ccgcgaatga aaatggaacg ttcagtcaac ttgagccact ctgaattcta
      241 tttcataaga gtctcttcaa cgggtgtgga atctcttccg gctagtaaca aatacgaata
      301 gagtgctatt ccggttagga ttgcaatgcc gtacttaatc tttggatcaa tgggagctgg
      361 tgacacaaag ccttcgatag ttccagcgat cacaaggaat actgcgacac ccaacatcaa
      421 acgtacagct tcagcgcctc tcgccttgag tgcgtctgct cgcgacagat cgccaggcat
      481 cagcatggca cttccaatca agagcccggc accgcccgca atgaaaatac acgacaactc
      541 gatcacgcca tgaccaacca tgaacgtcag caggtcgttt ccgaaaccgg cgcgatatgt
      601 tagagccagc actgctccca tattggctcc attaaaaccg aggtaataaa cggtgccgag
      661 ccctaacatg gcaccgaacg caaacgtgta gatcgtgacc cggatgttgt tctgcatgat
      721 gaacgctgcg ccgatctgat tcttatcgtt aaggtcttcc caccagggcg tacgagtctc
      781 gatgttgctt tctcgaaacg cagggttaac gccaactaac tcagaaaatt ccgggtcata
      841 acgtgtcgcg acaaagctga agaccgcaaa cgaggcgaag atagcgaatg agaatgccgt
      901 gtaacgccag gtgcgacgaa acgttcgtgg taactctcga gcgaaataat tacgtattcg
      961 cttgccaccc tgtgcatctg ctcgatagat tcgtccgtga gcacgaataa ccaaactgtt
     1021 gagatagttg actaatcggg gatctcgcga ttccgctcgt gcgatagcaa ggtcagaagc
     1081 cgtgcgacga taaatgcggc ccagttctcg aacctcatct ttgcggagct ttttgagaga
     1141 cgagcgatca agaagtttca ttagctcttc tagtcgctgc catgcatttt tccgttggtt
     1201 tatgaagcga tcggccggca cataaagcaa gatacgtcga aataaaagaa agtggaagaa
     1261 aaaagataag cagtatgact gggctgatcg agtgcgttcc gaattttagt gagggccgag
     1321 acgcagcggt cgtcgagatg attcgtagtg ctattctgcg tgtccctacc agctgtgtgc
     1381 tcgatactca cattgatgca gatcacaacc gttccgttat tacatttgtt gcacacgcgg
     1441 accacgttgt agaggccgcc ttcagtgctg tgaagcaggc tgcggaactc atcgacttgc
     1501 ggaaacatat tggcgaacac cctcggctcg gagcgactga tgtattgccg tttgtaccga
     1561 ttaagaatgc aacaatggaa gattgtgtaa ggctggcgca cgaagccggc aaacgaattg
     1621 cagccgagct cgcgattcca gtttattttt acgagcgagc ggccctgcgc gaagatcgcg
     1681 tgaatcttga agatgtgcgg cggggcgctt ttgaattact gcgagatcag ataacgacgg
     1741 tgacctcaag gataccggat gagggtgccg cgcgcattca cgaaagtgcc ggggccatca
     1801 taattggagc acgcccattt ctaattgcgt ttaactgtgt gttggcgagt gacgatcttg
     1861 cggctgcaaa acagatagca aaggcaatca gagcacgaag cggaggcttg cctttcttaa
     1921 aagcgcttgg ctttaagtta gcttcgcgaa accgcgttca ggtatcaatg aacctgttga
     1981 actatcgagt tacaggaatg cgagaggcgt acgaagctgt gcgacgtgag gcagagaagg
     2041 tgggaataga agttgagagc gccgaaattg taggacttgt accccaggat gcactgaatg
     2101 agtcggagga gtattttcaa aaacttgacg agtcttcgcg gttgaaaatt ttggaaaacc
     2161 aactcgcacg ctgtggaccg tttgaggcgt agcccgccga cgaatagtgg ctcccgcact
     2221 ccgtcaggag tgcaatgttt atagcccgcc gacaaatagt ggctcccgca ccccgtcagg
     2281 ggtgcaatgt ctattaacca cgagacgaga gcttggttat gctgcggcta aacatggcac
     2341 tcctaacgga gtgcgaggct ttgtccttac cgtggtctat aaacatcgca ctcctacgga
     2401 gtgcaaagct ttgttcttcg tggtctataa acataactct ccattaggag tgggttttta
     2461 cgatccaagc cttattaact cgcgacggag tctttgatgc ttctgcgtct ctcgcagata
     2521 tctcgtttca tcaactcccg ctttggctcg aattattgaa atcaactgcc gcttctcatg
     2581 ttcttcccat tcccgcaagt cgcgaacgag cgaaagtaca agcgcaaagg cagagaatgc
     2641 ctgttgctgc gacttgttta gttgactgct ctcaacgtta agggcgcctg caaccgtgtt
     2701 aagcgtcttc tttctcatca acgatgcgtc cccgttaaaa gcctgggcca tccgtttctg
     2761 tacacgcatc ccgatatttc tcacctggaa tctgtcccaa tcttcttgcc tgttcgcatc
     2821 aagctcgaag atcattggag cacgggccag ctcgcgcaga gttgctgcgg atgatcgata
     2881 gttctttttt gtactgagct tcttctcttc tttcgccgtc aattttctca cggcaggatc
     2941 tgtggatcga aaaccgagtt tacgatagaa ccagaatgcg cccgattcaa tcccttcctc
     3001 gttttcgtac ccgatctgat aaggatcgag tgagaaagtt gttacgcccg caatctggcg
     3061 catcacgttg agcgtctgcg cgtagatcca tgcagtttcg ccttcccgaa aggtgtagta
     3121 cagattgaag ccactctcca ttttttcgca aagtgaaagt ccttcgaagt acccgatcgg
     3181 cacagcattc cgataaatca tcgccgcatg ataggtccgc agcggcaaac gccgttcggc
     3241 aggcaatcca ataacatgaa ggcaaaggcc ccgatcgagc cttacgtcaa acacacgttt
     3301 tggatctcca tgggtaaaac cgtaaagctc tcgataacgc acagtagacg catctctcgc
     3361 tagatcaagc gcgcgctcac cttccgtcaa actgagcagc ctgctggtag agaccggctt
     3421 actgagctca tgacgaagga ttacgtcacg acgttgaatc agtggactac gctgaaagaa
     3481 gatcggttga ttgttagtgg aacggaggcc ggttctagtg gatcgatgac cgggtgtcca
     3541 ccgaacatat aactgctgag cgttgtagat ctcagccttc tctttgtcag ttagcggcaa
     3601 cttgtcgaag cgagataaca accattcaac ttcggatgtg ccatttcgtg cctgccggag
     3661 ccactcacga taagggatat tagcctcgac aaaggagtcc tcttccaata gaggcatgaa
     3721 tctgggccag gcatcagcaa ggcgattctc atcctcgaac cagtcccagt ccacttgtat
     3781 ttgttcagga tatttcctca ccaaccaccg aactatatca aagctaaacg tgtcgctgac
     3841 ggaatcgccg aagatgccgg agatctccgg gtgcattatt ggcgataggt cagcgtcgtg
     3901 ttcccttagg aattttattc gttggtcgaa agtgcgaagc tgggcttcaa ccgctctgac
     3961 agttggacca tcatgggggt aggcgcgcag aaaaaggagt gtctcgtgat agaggaccag
     4021 cgtttctgga tcggtaaacg ttaactgccg cagctggccc aggagagttc cgatagaacg
     4081 tgcggcagcg accgagaagt cggatttaag taagcttaat tgctcgacga gacgttcgtt
     4141 aggtgagcgc ggcacgaatg ttggtatgta gatctattag gctcggcgga tgaactttgc
     4201 ggagatccat cctacaagta ttaatcccga aagaaccagc cagttatttc tctcgccgta
     4261 agtaacacct ggataatggt tactaatcat gatcaacacc acaggaactg ccatgaaagt
     4321 gttttgtttc gagcgaagtt tcgcctgaga cgcgagcttt tcatctggac gctgcccatt
     4381 tttcagtgct tcgatcatct tgcgttgcgc cggcaggatt ctttgccaaa cattcgcggc
     4441 cataatggtc ccaaatactg ctccgacatg aatgtatgca gcgcgcccac taaagatgtg
     4501 cgtcaacgcg taacaaagtg caacgatcag aatataggcg atgacggcaa acactcgctc
     4561 attgccgcca agcggcgaca acatcattac gtcgtaaacg atccacccgc cgaccaggac
     4621 acctataccg atcaagacgg cgactccaac gctcaggtca cgaacgtcca catcaacaag
     4681 tgcaccaccg ccgagataga aaatcagaat gagtagcgct aagccgctga gccaggttgt
     4741 agctgcttcc caacggaacc agtgcaacgt gcgagaaacg agatcgggaa ctttgcgtcg
     4801 ttcaaccaca tagaacccac cgctatggac catccaaact tgcggcggtg t
//
LOCUS       DASSFP010000163         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1817400, whole genome shotgun sequence.
ACCESSION   DASSFP010000163 DASSFP010000000
VERSION     DASSFP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817400"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFH91_03535"
     CDS             <1..352
                     /locus_tag="VFH91_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HET6862085.1"
                     /translation="LLIEGILSVIAGVIAFAIPGITALFLLGLIAGWAIITGVIEIVA
                     AVQLRKEIQGEWLLALGGITSILFGVLLLLNPAAGALAVLWLIGIYAILFGIILVLLG
                     LKLRGMLHVSATTV"
     gene            complement(419..1045)
                     /locus_tag="VFH91_03540"
     CDS             complement(419..1045)
                     /locus_tag="VFH91_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6862086.1"
                     /translation="MAHKILTIDDHEVVREGVKGLFSSGLAEFGEARNGPEAMSLIRE
                     QDWDIAILDISLAGRSGLDVLKEIKQTRPKLPVLMLSMHAEDQYAMRAFKAGASGYIS
                     KASSRDELRQAVLKVINGGRYVSPSMAERMVLDLNVRERAGHEHLSDRELEVLCLIAS
                     GKTVGEIAKGLSLSDKTISTYRRRILDKMQLNTNAELTYYAIRNGLVE"
     gene            complement(1054..>4119)
                     /locus_tag="VFH91_03545"
     CDS             complement(1054..>4119)
                     /locus_tag="VFH91_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6862087.1"
                     /translation="SIKYILLSATSVRDEQGKFLMSRSTLYDITERKRAEELVRKSEE
                     KFAKAFRSSPIGVSVTRMSDGMYVEVNDTLAKSFGYSREELFGKTTLDLGIWVDLKDR
                     KRLVQALEQNGSVRNLELKFRTKQGDVVISEYSAEVIELGGEQCVLAVISDITERRHV
                     EEALLTNEGLLRQFVKHTPAAIAMFDNEMRYLQVSDRFLKDYHLEGKDILGKFHYEIF
                     PHIPERWREVHQRILEGSIERCDEDPYIERDGTLGWLQWESLPWRKAGGEIGGLILFT
                     QVITERKRAEKALKASEERFAKIFNLSPYRMGIIRRADGVILDVNDCWVRETGFTREE
                     TVNRPVVELDELLDQKTRDSIRSNLLEAQSLKSFEGSIRTKTGEKRFTLSSAALVDFD
                     GESCYLWAANDITDRKRVEEEKRHLIRDLNERVKELTALHQTARILQDDTKSVLGLLE
                     DIVSFLPPAWQYSEVTAARICFGDLEVKSHNFKETQWSQLVKFTAGGQKGEIEVVYLE
                     ERPQADIGPFLTEERSLLDSAAEMMSSALNRRYAQEALKESEEVFRTLAETVAAGIYI
                     YRKSKFVYVNPVAERLTGYSRKELLGMSLLDLIHPEFHDSVRERISTRPNGGMAQSHF
                     EDKILTKSGEERWMDVTAATTIFRGEPAVIVTTFDITARKRAEEDLLQSEERYRTLFE
                     TAPNAVEVFDSSLKLVMTNERGVKLYGFEQADDLIGKNVFDFVSPEDRKRVEGLITEM
                     QKSGKPCVFESKGVRRDSTHFDLETRATVIPNSHSQNPSLLTVKTDITERKRAETALK
                     ISEEQLRALSAKVQSTREEEGARIAREIHDELGAALTGLKWELEEIDSQLVDSSTASS
                     FDDLRKRIGTMTGLIEATISTVRRISSELRPGLLDDLGLVAAIEWQGQQFQTRTSIKF
                     HFDSNLETIEISRDGATAVFRIFQEVLTNVLRHSHASNIFVRLFARKRRLVLDVKDDG
                     RGITEREKKNTRSLGLLGMKERALLVGGNVEIQGARGKGTQVVVRVPLN"
BASE COUNT          953 a         1110 c          927 g         1129 t
ORIGIN      
        1 cttattgatt gaaggcatcc tgagcgtaat cgctggagtg attgccttcg caatacctgg
       61 catcactgca ttgttcttgc tcggattaat tgccggttgg gccataatca cgggtgttat
      121 tgagattgtc gctgcggtcc aactgcgtaa agagattcaa ggggaatggt tactggcgct
      181 gggtggcatt acctcgatac tttttggagt tctacttttg ctgaacccgg cggccggagc
      241 gctggccgta ttatggttga tcgggatcta cgcaatactc tttggaataa tcctggtatt
      301 gctcggattg aagctacgag gaatgttgca cgtttctgcc accacggttt gatcttttgt
      361 gctccgttga atgccgcgtt gcaagatggg ggcctacgga cggggaccct acaagaattt
      421 attcgacaag accgttgcgt attgcgtagt aggttagttc cgcattggtg ttgagttgca
      481 tcttatcgag aatgcgacgg cggtaagtac tgattgtctt atcgctgaga gataaacctt
      541 tggctatctc cccaacggtt tttcccgagg caatcaaaca caaaacctcc aattcgcgat
      601 cggaaagatg ttcgtgaccg gcgcgctcgc ggacatttag atcaagcacc attctttctg
      661 ccatcgaagg actgacataa cggcccccat taattacctt taagaccgct tgccgtaact
      721 catctcgtga actcgcttta ctgatataac ctgaagcccc tgccttgaag gctcgcatcg
      781 catattggtc ctcggcgtgc atgctcaaca tcaatacagg tagttttgga cgcgtctgtt
      841 tgatctcttt aagcacatca agtccactgc gccccgccaa cgaaatatcg agaatggcaa
      901 tatcccaatc ctgttcacgg atcaggctca tcgcttccgg accatttctg gcctcaccaa
      961 attctgccag acccgacgaa aacagacctt tcacaccttc gcgaactact tcgtgatcgt
     1021 cgatggttaa gattttgtgc gccattccac tccttaattc aatggtacac gcaccacaac
     1081 ttgagtgcct ttgccacgag caccttgtat ctcaacatta ccgccaacca gaagagcccg
     1141 ctctttcatg cccagcaaac caagcgaccg agtattcttt ttttcgcgtt cggttatacc
     1201 ccgtccatcg tctttgacat cgaggaccaa ccgtcgtttg cgagcgaaca gtcttacgaa
     1261 gatattgctg gcatgtgagt ggcgcaagac gttcgtcagc acttcctgaa aaatgcgaaa
     1321 tactgcggtc gctccatccc gactaatttc gatcgtttcg agattggaat caaaatgaaa
     1381 tttaatgctg gtccgtgttt gaaactgctg gccctgccat tcgatggctg ctaccagtcc
     1441 taaatcatcc aacagaccag gccgtagctc tgacgagatg cgacgtacag tcgaaatggt
     1501 cgcttcaata agaccggtca tcgttcctat ccgcttacgc agatcgtcga aacttgaggc
     1561 ggtactacta tccacaagct gactatcgat ctcttccaac tcccacttca gacccgttaa
     1621 ggctgcacca agttcatcat gaatttcgcg tgctatgcga gccccttcct cttcgcgcgt
     1681 cgactgcact tttgcggata atgcgcggag ctgttcttcc gaaatcttca aggcagtctc
     1741 agcgcgcttg cgctcagtaa tgtcagtctt cactgtcagc aaggacgggt tctgagagtg
     1801 tgagtttgga atcacagtcg ctcgcgtttc caggtcgaag tgggtgctgt cacgtcgtac
     1861 acctttggat tcgaacacgc aaggctttcc agacttctgc atttccgtga tcagtccctc
     1921 gactctcttt cggtcttcgg gactgacaaa atcaaaaacg ttcttgccga tcaaatcatc
     1981 ggcctgttcg aaaccataaa gcttgacgcc tctctcattt gtcatcacca gtttcaaact
     2041 tgaatcgaag acttcgacgg cattcggcgc tgtctcgaac agtgtgcgat atcgttcctc
     2101 actctgtagt aaatcttctt cggcgcgctt tcgtgcggtg atgtcgaagg tcgtcacgat
     2161 aactgctggt tcgcctctaa aaatagtagt ggcggccgta acgtccatcc atctttcttc
     2221 gccactcttc gttagaatct tgtcctcaaa atgcgattga gccattccac catttggtcg
     2281 tgtcgaaatt ctttcccgaa ctgaatcgtg aaactcggga tggatcaaat ccagcaaact
     2341 catgccgagc agttctttcc ttgagtatcc agttaacctt tcagcgaccg gattgacata
     2401 gacaaacttc gactttcggt aaatgtagat tcccgccgct accgtctctg ccagagtacg
     2461 aaatacttct tcactttcct taagagcttc ctgcgcataa cgccgattca gcgctgaact
     2521 catcatctct gccgccgaat cgagcagact tcgctcttcg gttagaaatg gtccaatatc
     2581 agcctgaggc cgctcttcaa ggtacacgac ttcgatttca cccttttgcc caccggctgt
     2641 aaatttgacg agctgtgacc actgagtttc tttgaagttg tgcgacttga cctcaagatc
     2701 tccaaagcaa atcctggccg ccgtcacctc tgaatattgc caggcaggag gcagaaacga
     2761 gacgatatct tcgagcagac cgagaacgct ttttgtatca tcctggagaa tccgggcagt
     2821 ttgatgcaga gctgtaagct ctttgacacg ctcgttcaga tcgcgaatta gatggcgctt
     2881 ttcttcttca actcgtttgc gatcggtgat gtcgtttgcg gcccacaggt aacacgactc
     2941 tccatcgaaa tcgaccaggg ctgcagatga caaagtaaac cgcttttcac ccgttttagt
     3001 tcgaattgag ccctcgaaac tcttcagcga ctgcgcttcc aacaaattag agcgaatgct
     3061 atcgcgtgtc ttctgatcga gaagctcgtc caattcaacg accggccgat taactgtttc
     3121 ttctcgagtg aatcctgttt cgcgaaccca acagtcgttg acgtcaagaa taactccgtc
     3181 ggcgcgccgg atgatgccca tgcgatatgg gctcaagttg aagattttgg caaaccgctc
     3241 ttccgatgct ttaagagctt tctcagctct cttacgttct gtgatgactt gagtgaaaag
     3301 aataagaccg ccaatttcgc ctccggcctt ccgccacgga agactttccc attgcaacca
     3361 accaagggtt ccatctcgct caatgtacgg gtcctcatca cagcgttcga tggagccttc
     3421 taatattctt tggtgaactt cgcgccatct ttcagggata tgcggaaaga tctcatagtg
     3481 gaacttgcca agaatatcct tgccttcaag gtgataatcc ttcaggaaac ggtcgctaac
     3541 ttgcagatac cgcatctcat tgtcgaacat tgcgatcgcc gcaggcgtgt gcttgacgaa
     3601 ctgtcgtaac aacccctcat ttgtcaaaag cgcctcttca acatgacgac gctcggtaat
     3661 gtctgagatt actgcgagaa cacattgctc accgccaagt tcaatcactt ccgcagagta
     3721 ttctgaaata accacatcgc cttgttttgt tctgaatttc agctcaaggt ttcgaactga
     3781 accattttgt tcaagcgctt gaaccagccg cttgcgatct tttaggtcca cccagatccc
     3841 taagtcgaga gtcgttttgc caaatagctc ttcgcgggaa tagccaaaag atttggccag
     3901 cgtgtcgttg acttcgacat acattccgtc agacattcgc gtaacactga cgcctattgg
     3961 gctcgagcgg aatgcctttg caaatttctc ttccgatttg cgtactaact cttcggcccg
     4021 ttttcgctcc gttatgtcgt acagcgtgct gcgactcatc aggaatttcc cctgctcgtc
     4081 gcgaacggaa gtggcgctca acagaatgta tttgatcga
//
LOCUS       DASSFP010000164         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1229159, whole genome shotgun sequence.
ACCESSION   DASSFP010000164 DASSFP010000000
VERSION     DASSFP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229159"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..3520)
                     /locus_tag="VFH91_03550"
     CDS             complement(113..3520)
                     /locus_tag="VFH91_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET6862088.1"
                     /translation="MRLSSIVKGNYICSCCSPTFFTPAKCATIPGQKDRQKDLRLRVA
                     TRLAMATVAILLFVSVGHGQGFGNIVGTVKDPSGAVIPNASITVTETGKNFSRTARTD
                     SSGYYVIGSLRPSDYNLTVDAQGFHIYEQKGVTLLADQTLTANVTMMVGAVSESVSIT
                     TAGELVDTSTSTLKQVIEQRSISELPLNGRNAAELSLLVAGTVSYPINPANPTGTFAG
                     GALQGTTKTFPGAVAIVTNGSRQNQVSYQLDGGNNVDEYTNVNQPFPMPDALQEFSVQ
                     TSNYSAQYGQNAGGVVNVITKSGTNQYHGNAFEFVRNSVLNARPWHAPSKDQLKRNQF
                     GGTFGGPILHDKTFFFGAYQQTIQRNLLAGQSANVPSAAARASASDPAVVQLLKHIPL
                     GVPTGNTNLNSATFFRPDQQDFREFTLRVDHSFSTKDQITFRYFYDHFTRNAVFDPDN
                     LLIYSDGSTIKSQNFLGHWSHVFKPSVINDFRFSYAPENATRGPADNAIDVTDLGVKL
                     PFQAPQKAIQQVRINGVFNFGDNPQASFIRNNLTWADDVSWVAGKHDLRFGGIIERSQ
                     VDLLNGFFQPAEFSFSSLPNFLAGKLTDYSGNLAFRQGAGEFKNNRNTFIGLYIQDNF
                     RFTRRLMFNLGLRWEPALPWREIKGRQEQFRLPDLIARRRSTQFPNAPPGVFFPGDEG
                     VPEDGVRPNYTSFAPRFGFAYDVFGDSKTSIRGGFGIFYDTRIPGIINNRFADLTPFS
                     PQFVLNTGVVNPGTFADPLCTLPATQTLQNCTNQSANYPFPFTYPPDKNIQFGLGTFV
                     LSWDPVNKYQPPTVYNWNLTIERQLPENVLVRAAYVGSHSSHLTETLNLNPRPVGTTS
                     STPTRLNAIAGSPLFSTVQQDLQDINASYNSMQLSLEKRMSRGFMILANYTWSKSLDD
                     LPPRAGVTGFDTSSALPWDDPNRHRFDYGPSEFDHTQRFVASWIWQLPDLKDKNSVLK
                     AFFGDWQLSGLVQAQTGRPMSVTQGADISGTGIGQDRGSFTGVDPYLSGPCAGVASCK
                     LWLNPAAFKLATDPSVKNTFGNVGKGSLRFPGYYAWDMGISKVFALTERYKIQLRGEF
                     FNLFNQVNFLSDEGTVNNFSTVSNGNFGRLRTAMDPRVGQLALKFIF"
     gene            3903..4172
                     /locus_tag="VFH91_03555"
     CDS             3903..4172
                     /locus_tag="VFH91_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862089.1"
                     /translation="MVRPSRRGLVVEEVVLRVQEMIRQDAYLRHSESANPRLRSAETF
                     CSASRIYSSDLFLARRLLEVNASGLGATNPTGQDMVVLAEEVTEM"
BASE COUNT         1048 a          999 c         1110 g         1065 t
ORIGIN      
        1 attagttcac cgcagagacg cagcgacgct gagataccgc agagatcgtt cttaacgtac
       61 ggggcgccgt ccgtggaggc tccccgatat taaacttcct ttagcccaca aactagaaga
      121 taaactttag tgcgagttgg cctactcttg ggtccatggc tgtgcgcaaa cggccaaagt
      181 tcccgttgct aaccgtggag aaattgttaa ctgtgccttc atcgcttagg aagttcactt
      241 gattgaagag attgaagaac tcgccgcgaa gctggatttt gtatcgctca gtaagagcaa
      301 atacctttga gattcccatg tcccaggcgt aataaccagg aaaacgcaaa ctgccctttc
      361 ctacatttcc aaacgtattc ttaacgctcg gatccgtcgc cagtttgaaa gccgcagggt
      421 tgagccacaa cttacaagaa gctacgccgg cgcaaggtcc agacagatag ggatctactc
      481 cggtgaagga tccgcggtcc tgacctattc ccgtgccgga tatgtcagca ccttgcgtga
      541 cgctcatcgg acgaccagtc tgggcctgca cgagtccact taattgccaa tcaccaaaaa
      601 aggctttcag cacagaattc ttgtctttta gatctggtaa ctgccagatc caggaagcaa
      661 caaagcgttg ggtgtggtca aactcagacg gcccatagtc gaagcggtgc ctgtttggat
      721 catcccaggg taatgcagat gaagtatcga aacctgtgac gccggcgcga ggtggaagat
      781 cgtccagact cttggaccag gtatagtttg ccaggatcat aaaaccgcgc gacattcgct
      841 tctcgagaga gagctgcatt gaattgtagc tcgcattaat gtcctgaagg tcctgttgca
      901 cagtactgaa tagtggactt ccagcaatcg cattcaatcg tgttggagta gacgaagtag
      961 tacccacggg tcgtggattt aagttgagag tttcggtgag atgactgctg tgagaaccga
     1021 cataagcagc tcgtacgagc acattctctg gcaactggcg ttcaatcgtc aggttccagt
     1081 tgtaaacggt tggaggctgg tacttgttga ccggatccca acttaagaca aaggtcccaa
     1141 ggccaaactg tatgttcttg tctggtggat aagtaaaagg aaatgggtag ttcgcagatt
     1201 gatttgtgca gttctgcaat gtctgtgtgg ccggaagagt gcaaagagga tccgcgaagg
     1261 tacctggatt tacaacgcct gtgttgagca caaactgcgg gctgaaaggc gttaagtctg
     1321 caaatcgatt attgatgatt ccgggaattc gagtgtcgta gaagatgccg aaacctccac
     1381 gaatgctcgt tttgctatca ccaaaaacgt cataggcaaa tccaaagcgc ggcgcgaagc
     1441 tagtatagtt tggccgaact ccgtcttcgg gaactccttc atcacccgga aagaaaactc
     1501 ctggaggtgc gttgggaaac tgtgtggaac gccggcgggc aatcaaatca ggaagtcgaa
     1561 actgctcctg acgtcccttt atctcacgcc agggaagagc aggttcccaa cgtagcccga
     1621 gattgaacat taggcggcga gtaaatcgga agttatcctg aatgtagaga ccgataaaag
     1681 tattgcggtt gttcttgaac tcaccagcac cctgccggaa ggccagattg ccgctgtagt
     1741 cagttagttt tccggctaga aaatttggga gcgaggaaaa gctaaactcc gccggctgaa
     1801 aaaatccgtt gagcaggtct acctggctgc gttcgatgat gccaccaaag cgtagatcat
     1861 gctttcctgc cacccagctg acgtcatcag cccacgtcaa attgtttcga ataaagctgg
     1921 cttgtggatt atctccaaaa ttgaagaccc catttattcg aacctgttga atcgctttct
     1981 ggggtgcctg aaacggaagc ttgacgccga ggtcagtcac atcaatcgcg ttatcggctg
     2041 gtccacgagt agcgttctcg ggagcgtaac tgaaacggaa atcgttgatg acggatggct
     2101 tgaatacgtg actccagtgt cccaggaagt tttgggattt gattgttgac ccatctgagt
     2161 agatcaacaa attgtccgga tcaaagactg cgtttcttgt aaagtgatcg tagaagtaac
     2221 gaaaagttat ttggtctttt gtcgagaatg agtggtccac gcgtaaagtg aattctctga
     2281 agtcctgttg atcgggacga aagaatgtcg cactgttgag gtttgtgttt cctgtgggga
     2341 cgccgagcgg tatatgtttc agaagttgaa cgacggccgg atctgatgct gaggctctgg
     2401 cggccgcgct tggcacattt gctgattggc cagccaacag attgcgctga atagtttgtt
     2461 gataggcgcc gaagaagaat gtcttgtcat gcagaattgg accaccaaac gtcccgccaa
     2521 actgattgcg tttgagttga tctttgctcg gcgcgtgcca tggtctggca ttcaaaaccg
     2581 agttacgcac aaactcaaag gcattgccgt ggtattggtt agtacctgac ttcgtaatga
     2641 cattcacgac accgccggca ttctgaccat actgcgcgct atagttgctc gtttgtacac
     2701 tgaactcctg tagtgcgtcc ggcattggaa aaggttgatt gacgttagtg tattcgtcga
     2761 cattgtttcc accatcaagt tggtagctga cctgattttg acgggaccca tttgtcacga
     2821 tggcaaccgc gccgggaaat gtttttgtcg taccctgaag tgcgccacca gcgaaggtgc
     2881 ccgtgggatt tgccggattg atcggataac taacggtccc tgctacaagc aagcttaact
     2941 ctgccgcgtt cctgccattt aggggcaact cagagatgct gcgttgctca atcacctgct
     3001 ttagtgttga ggttgaagtg tcgactaact ctcccgcagt cgtaatggat actgactcgg
     3061 agacagctcc caccatcatg gtaacgtttg ctgtaagagt ttgatcggcg agtagagtca
     3121 cacctttctg ctcgtagatg tgaaagccct gagcatcaac ggtcagattg taatctgatg
     3181 gccgaagtga gccgatcacg tagtaaccgc tcgagtcggt tctggccgta cgagagaaat
     3241 tcttgccagt ctcagttacc gttatgcttg cgtttgggat tacagcgcct gaaggatctt
     3301 taacagttcc cactatgttt ccgaagccct ggccgtggcc caccgaaaca aagaggagaa
     3361 tagcgactgt tgccatcgcg agccgggtgg cgactcgcaa tctaagatcc ttctgacgat
     3421 ccttctgacc tgggattgtg gcgcattttg ctggggtgaa aaaagtgggc gaacaacaag
     3481 agcagatgta gttgccttta acgatgctac tcaatctcat gatcttctcc tcccgctggg
     3541 cgagtctggc ggttgttgaa agtgccgagg tctaacccgg cgaatgaaag ttattccacc
     3601 tggcaacggg cgataggctc tccttttaca agaccgactg cgccttaaaa cctcatcgat
     3661 tgcgcgacca tttaacttta tacaagacag attggtatga ccattatggc tatttaaggg
     3721 ccttgtatca cgctcgattc tcgccgtcaa gagttttgtt cttaaaataa acaaattggc
     3781 gtatctactt gcaagctcag tgaaacttga gagttgacat cttctgcaaa agggtggtac
     3841 acgtccacag tggcgatctg ttcacctaaa tttgtcgtgt caatttaagc cgtatttgga
     3901 aaatggtaag accatctcga cgaggtctag ttgttgaaga ggtcgtactc cgggtgcagg
     3961 agatgatccg ccaggacgcc tacctacgtc actccgaatc cgccaaccct cgattgcgct
     4021 ccgctgagac tttttgcagc gcttccagga tttactcttc cgatcttttt ctcgcgagga
     4081 gactcctgga ggttaatgcg tcaggcctcg gtgcaacaaa tccaaccggg caggacatgg
     4141 tggttttggc agaagaagtt acggagatgt aggcggcgct ggaacacctc ttacttgccc
     4201 agagagcaaa ttgaatcaga aa
//
LOCUS       DASSFP010000165         5980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1372720, whole genome shotgun sequence.
ACCESSION   DASSFP010000165 DASSFP010000000
VERSION     DASSFP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372720"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /gene="nuoL"
                     /locus_tag="VFH91_03560"
     CDS             complement(<1..1800)
                     /gene="nuoL"
                     /locus_tag="VFH91_03560"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET6862090.1"
                     /translation="MYKYIWLVPLLPLIGAAINGLFGRKFRFSEKLIGGIAVGSVALS
                     FLISLTAVFSYGFSSQPEWPNPYITSKDGFSFNWIPGGAVSINQGAAAKQAIRAQHEL
                     EQKQATEHAAAETPETPTAALLNIEWSYQLDPLSSIFMLIVTGVGLCIFIFATGYMHG
                     EAGFYRFFAYMGLFMFAMLVLVMGSNFLMMFVGWEGVGLCSYLLIGYYFDRQEAANAS
                     RKAFITNRVGDFGFALAIFGVIATFGSAQYVDVFNQAKAYPIELIGHWGILSWIALGL
                     FIGACGKSAQLPLHVWLPDAMAGPTPVSALIHAATMVTAGLYMVTRTNVIFQHSQTML
                     MVVALVGAATAIFAATIGITQNDIKKVLAYSTVSQLGFMFMACGVGAFAIGIFHVMTH
                     AFFKALMFLGAGSVIHGMHHEQDMRRMGGLKKYMPYTYWTFFAGWLAICGIIPFSGFW
                     SKDEILWKAASTTFIPLGWLVWLVGTIAATCTAFYMTRLMAMTFWGKEKFLGHVAGGE
                     ADEAHASAYDKGLTQHDAFKPSAGDRHRHQPGEHVGPTSPDHGVHLPHESPKAMWVPL
                     VVLAVLATIGGFVGVGPAFRFVTGTEHPGGRLNI"
     gene            complement(1836..2243)
                     /gene="nuoK"
                     /locus_tag="VFH91_03565"
     CDS             complement(1836..2243)
                     /gene="nuoK"
                     /locus_tag="VFH91_03565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET6862091.1"
                     /translation="MSSFLLSACLLLIQESSGGRLAQSWDAVKHPDLSKFLVIGALLF
                     IIGVAGVLTRRNIIVIFMSIELILNAANLNFIAFSRYLEETGNANAIAGQIFVVFIIV
                     VAAAEAAIGLGIVIALYRNKETIWVDEIDLLKW"
     gene            complement(2245..2931)
                     /locus_tag="VFH91_03570"
     CDS             complement(2245..2931)
                     /locus_tag="VFH91_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET6862092.1"
                     /translation="MTLSGWEAVFFWVFGLAAIFAGLLTVMARNAVHSALFLISSLLS
                     VAALFILLGAEFIAGVQILVYVGGVMVLFLFVIMLVNVGAEEEGKDEIFNKPRQVATS
                     LVFCLLLVGSLLWAVSNGSKVLMAKTLNPDGTPIREWVQDPTLRASATGTSVITTATQ
                     VLGGALYRYGSLPFEIASVLLLVAIIGSVMLARTLKQEASVDDLAPEVLQEEILNEPE
                     LANVEQLTIE"
     gene            complement(3049..3579)
                     /locus_tag="VFH91_03575"
     CDS             complement(3049..3579)
                     /locus_tag="VFH91_03575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET6862093.1"
                     /translation="MEVKTPKPTLLQKLKRFLFMDLIKGMSLTLKYNLGAITDKDSVA
                     GKGIYTEQYPKVRPDVAPRFHGAPRLNMDPETHDTLCIACNLCAIACPEDCIDVIPMD
                     VEIIVAGKPRRKKVLDEFIFDTSRCMFCALCQEACPTSCLELTQDFELATYSRAGFVW
                     NREMLEQGRETVKYTS"
     gene            complement(3624..4931)
                     /gene="nuoH"
                     /locus_tag="VFH91_03580"
     CDS             complement(3624..4931)
                     /gene="nuoH"
                     /locus_tag="VFH91_03580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET6862094.1"
                     /translation="MLLFGPINVLNDLLLRFFSADTINYFVWPLIQIGLVVTLVAAWV
                     AYATYLERKISAFMQARLGPMRVGPWGLLQPIADGIKLLAKEDFIPEKADRWIFFFAP
                     YIAVASAFIVFSVVPFGPDWAVIADVNIGLLLVLAVSSVGVLALILAGWSSNSKYALL
                     GGLRSSAQMVSYEVAMGLSLIGALMFARTLSLSGMVYAQGSDSIWYIVYQPAGFLIFL
                     ISGIAENNRAPFDLPEAESELVAGFHTEYSGMRWSLFFMAEYAAMVVVAAVAATVYLG
                     GWYFPFVDRLEQNGYHNLYVFVSLLVFLIKASIILYVYFWLRWTLPRFRYDQLMDIGW
                     KWLIPSALINIVLTALAVFLVQALDGWRGMKTIEAVSQGLNLTWAGKIIMFATGLLSV
                     FITGFLLSRINWRSRDFNLKTQRRNIRLVNVAKGKPAVEAVQQ"
     gene            complement(4939..>5980)
                     /locus_tag="VFH91_03585"
     CDS             complement(4939..>5980)
                     /locus_tag="VFH91_03585"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET6862095.1"
                     /translation="GVLRVILKLDGETVIDLDCDIGYLHRGMEKIAENRMYTMIAPYW
                     DRLDYIAAVSNGLVWVETVEQMMGLVVPPRAHYLRTILTELNRIASHLLWLATHALDI
                     GAVTVLLWALREREEILKIFERQFGARLTCHAWRVGGMINDAYDDFHKDVRQFVNNFP
                     KRLDEYETVLSENRIWLSRTVGIGVISAADAVAIGLSGPALRGSGVAWDIRKSRPYAA
                     YGDMDFDIPVGENGDTYDRYLVRLEEMRQSRRIIDQALENLPDGPVNAKLPKVVKPPV
                     GDYYHSIEGPKGEIGCYLVSDGSAQPWRVRVRPPSFVNLQALRQMCLGHLVADVVAII
                     GTLDIVLGEIDR"
BASE COUNT         1651 a         1615 c         1408 g         1306 t
ORIGIN      
        1 gatgtttagt cttccacctg gatgctctgt gccagtaaca aaccgaaagg ctggtccaac
       61 accgacgaat ccgccaatgg ttgcaagtac cgcaagaacc acgagcggaa cccacattgc
      121 cttaggcgac tcgtgcggaa gatgtacccc atgatcgggc gaagtgggtc caacatgctc
      181 tccgggttgg tgacgatgtc gatcaccagc ggatggctta aaggcatcat gctgagtcaa
      241 acccttgtcg taggctgaag catgagcttc gtcagcttca cctccagcaa catgtcctaa
      301 aaatttctct ttcccccaga acgtcatcgc catcaagcga gtcatgtaaa acgcagtgca
      361 agttgcggcg atagttccga ccagccaaac cagccagccg agcggtataa aggttgtaga
      421 agccgctttc caaagtatct cgtctttgga ccagaaaccg ctgaacggaa tgattccgca
      481 gattgccagc cagcctgcaa aaaacgtcca gtaggtgtag ggcatgtact tcttaagccc
      541 gcccatccgt ctcatgtcct gctcgtggtg cattccgtga atgacactgc cagctccgag
      601 gaacatcaga gctttgaaga acgcatgtgt catgacatgg aatatgccga ttgcaaaagc
      661 accaacgcca cacgccataa acataaaacc gagctgtgaa accgtcgagt aggccagtac
      721 cttcttgatg tcgttttgtg tgattccaat ggtcgccgca aaaattgccg tcgcagcgcc
      781 tacaagcgct acaaccatta gcatcgtttg cgaatgctga aagatgacat tggtccttgt
      841 aaccatgtac aagcccgcag tcaccatcgt tgcagcgtga atcaaagcag agaccggagt
      901 tggaccagcc atcgcgtccg gcaaccaaac atgtagcgga agctgagcag acttaccgca
      961 agcgccaatg aaaaggccca gcgctatcca cgacaggatt ccccaatgac caattaactc
     1021 aatcggatat gctttggcct gattaaagac gtcgacatat tgtgcggagc caaatgttgc
     1081 gatcaccccg aagatggcca gcgcgaagcc aaagtctccg acccgatttg tgatgaatgc
     1141 tttgcgcgaa gcgttagccg cttcctgtcg atcgaagtag taaccgatta acaaatacga
     1201 gcagagaccg accccttccc aaccaacaaa catcatcagg aagttggagc ccattacgag
     1261 caccagcatc gcgaacatga acaggcccat gtaagcgaag aacctgtaaa aaccagcctc
     1321 gccatgcatg taaccagttg cgaagataaa aatgcacaat ccgacaccgg tgacgatgag
     1381 catgaagatc gacgacagcg gatcgagttg atagctccat tcgatattca gcaacgcggc
     1441 agtcggagtt tccggcgtct cagccgcggc atgctcagtc gcctgttttt gttcgagttc
     1501 atgttgagcc cgaatcgctt gtttggctgc ggcgccctga ttaattgaaa ctgctccacc
     1561 cggtatccag ttaaagctga acccgtcttt gctggtgatg tatgggttgg gccattcagg
     1621 ttgactgctg aaaccgtaag agaagacggc ggtgagacta attaaaaacg acaaggcaac
     1681 agagccaacc gcaattccgc ctatgagctt ttccgaaaac ctgaacttac gtccaaacag
     1741 gccgttgatc gcggcgccaa ttagaggcag aagtggcact aaccagatgt atttatacat
     1801 tgggtcttag tactctgagt ttccctggtc cattctcacc atttcagtaa atctatttcg
     1861 tcaacccaga tagtttcctt gttgcgatag agcgcgatga caatacctaa tccgatagcc
     1921 gcctcggcag cggcgacaac gattatgaag acaacaaaaa tttgtcccgc gatagcgttc
     1981 gcgttccctg tttcttcaag gtagcgcgag aatgctatga agttcaggtt ggcagcgttg
     2041 aggatcagct cgatggacat gaagatgacg atgatgttcc ttcgagttaa gacgcctgcg
     2101 acgccgatga tgaacaacag ggcaccgata accaaaaact ttgacaggtc aggatgctta
     2161 acggcgtccc atgactgggc gagacgtccg cctgatgatt cctgaatcaa caacaaacat
     2221 gcagaaagaa ggaagctcga catactattc aatcgtcaat tgttcaacgt ttgcgagttc
     2281 tggttcattg agaatttcct cctgcaaaac ctcaggtgcg aggtcgtcca cagaagcctc
     2341 ttgtttcagc gttcgggcca gcattaccga tccgataatc gcgactagaa gtaagacgct
     2401 cgcaatctca aacggcagcg atccgtaacg atagagagcc cctcccagta cttgagtcgc
     2461 tgtagtgatc acgctcgttc ctgtcgccga agcacggagt gtcggatcct ggacccactc
     2521 tcttattgga gtgccgtctg gatttagtgt ctttgccatc aaaactttgg aaccgttgct
     2581 cacggcccac agcagcgagc caactaacag cagacagaat acgaggctgg tagccacctg
     2641 cctgggcttg ttgaaaatct catcttttcc ttcttcttcg gccccaacat ttaccaacat
     2701 gataacgaac aggaatagga ccatcacccc gcctacatag accaggatct gaacgccggc
     2761 aataaattca gcgcccagaa ggatgaatag cgcggcaaca gaaagtagcg aggagatcaa
     2821 aaagagagcg ctatggaccg cattacgcgc cataacggta agcaagccgg caaagatcgc
     2881 cgcaagccca aacacccaaa agaatacggc ttcccaacca gagagagtca tataattgcg
     2941 aacgttctaa ctgtttattt caacgcgtgc cggcgtaaag atggtgctcc gccgacaact
     3001 acaaacctga tggatgcact gtaaaaacta aacccttttg gtagaacttc agctggtgta
     3061 tttgaccgtt tcgcgtccct gctccaacat ctcccgattc cagacaaagc ccgcgcgaga
     3121 gtaagttgct aactcgaaat cctgggtgag ttccaggcac gaagttggac aggcttcctg
     3181 acacagtgca cagaacatgc agcgcgacgt atcgaaaata aactcatcaa gaactttctt
     3241 gcgacgtggt ttcccggcga cgataatctc aacgtccatc ggaattacgt ctatacagtc
     3301 ctcgggacag gcgatagcgc aaaggttgca cgcaatacag agggtatcgt gtgtctccgg
     3361 gtccatattg agacgtggcg cgccatgaaa gcgcggtgca acgtctggtc taacttttgg
     3421 atattgttca gtataaattc ccttgccggc gacagagtct ttgtcggtga tagctccgag
     3481 gttgtacttc agagtaaggc tcatgccttt gatgaggtcc atgaatagaa agcgtttaag
     3541 cttctgtagc aatgttggtt ttggagtttt gacttccata ttaggcaccc cgatttgtat
     3601 aggacctata ggacgtattg gacctattgc tgaactgcct cgactgctgg tttccccttt
     3661 gccacattaa ccaacctgat attgcgtcgt tgcgtcttca aattaaagtc acgtgaacgc
     3721 caatttattc tcgacaataa aaaacccgtt atgaacacgc tgagcagccc agttgcaaac
     3781 atgatgatct tgcccgccca ggtaagattc agaccctggc taacagcctc aatcgttttc
     3841 attcctcgcc atccgtcgag cgcctgaacc agaaacactg cgagggcggt caagacaatg
     3901 ttaatcaggg ctgacgggat caaccatttc cacccgatgt ccatcaactg atcgtaacgg
     3961 aagcgaggca acgtccatcg caaccagaaa tagacgtaaa ggatgatcga agccttaatt
     4021 agaaagacca gtagacttac aaagacgtag agattgtgat atccgttctg ttcgagacga
     4081 tcgacaaacg gaaaatacca tccccccagg tacacagtag cagcaaccgc cgcaacaacc
     4141 accatcgccg cgtactctgc catgaaaaag agcgaccagc gcatcccgga gtactcagtg
     4201 tggaacccgg caaccaactc cgattcagct tcaggaagat caaagggcgc tcgattgttt
     4261 tcggcaatac cactgattaa gaagattaga aaacccgccg gctgataaac gatgtaccag
     4321 atggaatcgg aaccttgagc atagaccatg cctgagagcg acaacgttcg cgcaaacatc
     4381 aacgctccga tcaatgagag ccccattgca acttcgtacg acaccatctg ggcactcgaa
     4441 cgcaatccac ccagcaacgc atactttgaa ttcgaagacc agcccgccag gatcaacgct
     4501 aaaacaccga cactggagac agccagtacc agcagcaggc caatattaac gtcagcaatt
     4561 acggcccaat caggaccaaa aggcaccacc gagaaaacga taaaagcaga cgcgacggcg
     4621 atgtaaggcg cgaagaaaaa gatccaccgg tcggcttttt ccggaatgaa gtcctctttt
     4681 gccagcagct tgattccatc tgctatcggt tgcagcaagc cccatggtcc aaccctcatt
     4741 ggacccaaac gggcctgcat gaacgcagaa atctttcgct caagataggt tgcgtaggcg
     4801 acccaggccg caacgagagt gaccaccaac ccaatttgta tcagcggcca gacaaaatag
     4861 ttgatggtat ctgcactgaa gaacctcagc agcagatcgt tgaggacatt tattgggccg
     4921 aagagtaaca tcgcactcct atcgatcaat ttcccccaaa acaatatcca gtgttccgat
     4981 gatggccacg acgtcggcaa cgagatgtcc gaggcacatc tgtcttaacg cctgcagatt
     5041 cacaaatgaa ggtggacgta cccgcacgcg ccacggttgg gcacttccat cagataccag
     5101 gtaacaacca atctctcctt ttggtccctc gatggagtgg taatagtcac caactggagg
     5161 cttcacgacc tttggcagct tggcgttgac tggaccatcc ggcagatttt ccaatgcctg
     5221 gtcgataatg cgacgcgact gccgcatctc ttcaagtcgc accaggtaac gatcgtaagt
     5281 atcgccgttc tcacccaccg gaatatcgaa gtccatatcc ccatatgctg cgtaaggacg
     5341 cgatttccga atatcccagg caactccaga ccctcgcaaa gctgggccag aaagcccgat
     5401 cgccacggcg tctgcggcgg aaatgacacc aattccaact gttcgactta accagattcg
     5461 attttcagac agaactgttt cgtactcatc gagacgtttg ggaaagttat tcacaaactg
     5521 gcgaacgtct ttatgaaagt cgtcgtaagc gtcgttaatc attcctccga cgcgccaggc
     5581 gtgacaggta agtcgagcgc caaattgtcg ttcgaaaatc ttcagaatct cttcgcgttc
     5641 acgcaacgcc caaagcagaa ctgttactgc tcctatatcc agcgcgtgag ttgcgagcca
     5701 gaggagatgt gaagcaatcc gattcagctc agtaagaatc gtccgcaggt aatgagcgcg
     5761 aggcggtaca accagaccca tcatctgttc gactgtctca acccacacca atccatttga
     5821 aactgcggca atgtaatcaa gacgatccca atatggtgcg atcatcgtgt acatgcgatt
     5881 ttctgcaatc ttctccattc cgcgatgcag ataaccgatg tcacaatcga gatctattac
     5941 tgtttcacca tcaagtttta agatcactcg cagcacacca
//
LOCUS       DASSFP010000166         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_489038, whole genome shotgun sequence.
ACCESSION   DASSFP010000166 DASSFP010000000
VERSION     DASSFP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489038"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFH91_03590"
     CDS             <1..555
                     /locus_tag="VFH91_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET6862096.1"
                     /translation="RGEGFCVLNDVAVAIRVLRQESIIHRALVIDCDVHQGNGTATIF
                     SDDHLTFTFSMHGANNYPLFKANSNLDIELADGISDREYLSTLRSNLPHVLGFKPDLV
                     FYLAGADPYVNDKLGRLALSIEGLRERDSLVLRECYQHEIPVVTVMSGGYGKDINDTI
                     EIHCNTIRIVKEIFEGQATSRRFA"
     gene            556..1323
                     /locus_tag="VFH91_03595"
     CDS             556..1323
                     /locus_tag="VFH91_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET6862097.1"
                     /translation="MNKRLMRKLLKWFFLIVAGIVIGTIFYEALLFVRVFRLRGNNPS
                     STSLIDTRAQQAEAKGQQPRREQQWLPLDRISINLQRAVLAGEDTNFVYHHGFDYQAI
                     QKAWDQAQREQAKEAKKEGEDDDWLPNIPEFKRGASTITQQLAKNLYLSSERSFVRKG
                     QEAIVTLMLERCLTKRRILEIYLNVIEWGEGIYGAEAAAQHYFNKSASALSPTEAAFL
                     SAMIPNPRTVFNPQINPRRVARRQRIILRGMPYVKTP"
     gene            complement(1377..2552)
                     /locus_tag="VFH91_03600"
     CDS             complement(1377..2552)
                     /locus_tag="VFH91_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET6862098.1"
                     /translation="MKIPRTLWTAIAFASLLIVVPRVNGAAFLGKVIEVNEGDVITVS
                     NQNRPVRVRLLGVDAPELTQPFGDVAKQHLSDLVMDKIVLVQSAGYTKNYALIARVLV
                     GDRDICAQMIRDGAAWFDPKSPVLTPADGEIYTQSQIAARNERRGLWQVDNPVAPWDF
                     VKASKAQVSSASAPATPLSAPRKSANSGLTTEGLRWANIAASRPPSDNSKDAGENYAP
                     EVNGQWRTLRPAGEKFSVLVPVDGKSISLPVPSGDRLINFHIYMGRQNLSVYQVMWVT
                     GPSMGESDADAINGTMQGFLRGMASSFEARNGAGSFQCETPEPRTVSMKGYTGQEFNF
                     QGCTLPGVARVFTKLVGDDRRMYVFGAFYKEKDANIGKFLKSFEFVATENTTARNKN"
     gene            complement(2590..>2960)
                     /locus_tag="VFH91_03605"
     CDS             complement(2590..>2960)
                     /locus_tag="VFH91_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862099.1"
                     /translation="SMFKEMLQTVIERTEGGLGALIMGTDGIAVEKVLAEAGLDANLD
                     VAAAEFTSLIRNAQRAAHDTGLGGLKELVASLEGATLIMRMLSRDYFVVLALEAKGNL
                     GRARYELRKAELKLAKEFAV"
BASE COUNT          748 a          794 c          669 g          749 t
ORIGIN      
        1 cgcggtgaag gattttgtgt tttaaatgat gtcgcggtcg caatcagagt gctgcgtcag
       61 gaatcaatta tccatcgcgc cttagtcatt gattgtgacg ttcaccaggg caacggcact
      121 gccacaattt tttccgacga ccacctcacg tttaccttct cgatgcatgg cgccaataac
      181 tatccgctct tcaaagcaaa ctcgaacctt gatatagagc tcgccgatgg catttcagat
      241 cgcgagtacc tgagtaccct tcgcagcaat cttccccacg ttctcggttt caaacctgac
      301 ctcgtctttt atttagccgg cgccgatcct tatgtaaacg acaaacttgg ccgattggca
      361 ttgtccattg aaggattacg cgagcgtgac tcgttggtct tgcgcgagtg ctaccagcat
      421 gagatacctg tagtcactgt tatgtcgggc ggttacggaa aagacattaa tgacacgatc
      481 gagattcact gcaacactat tcgaatagtt aaagagatct ttgaaggtca agcgactagt
      541 cgaagattcg cgtgattgaa taaacgccta atgaggaaac ttcttaaatg gttttttcta
      601 atcgtcgccg gcattgtgat cggcacgatc ttctatgagg ccttactgtt tgttcgcgtt
      661 tttagactgc gcggaaataa tccgtcatca acctcattga ttgacactcg ggcccaacaa
      721 gccgaagcca aaggacagca accgcgccga gaacaacagt ggcttccatt ggacagaata
      781 tcaatcaatc tccaacgtgc tgtactcgcg ggtgaagata caaacttcgt ctaccaccac
      841 ggatttgatt atcaggcaat acaaaaagcc tgggaccagg ctcaaagaga gcaagcgaaa
      901 gaagcaaaga aagaaggaga ggatgatgac tggctgccca acattccaga gttcaaacgg
      961 ggcgcttcta cgatcactca acagcttgct aaaaatctgt atctttcgtc agagcgatcg
     1021 ttcgttcgaa aaggacaaga ggcaatagtg accctcatgt tggaacgatg tcttactaag
     1081 cggcgcatac ttgagatcta tttgaacgtc atcgaatggg gcgaggggat ctacggtgca
     1141 gaggcagccg cgcagcatta ttttaataag tctgcttcag ccctgtcgcc aactgaagct
     1201 gcgtttcttt cagcgatgat tcccaatcca cgcactgtct tcaatcctca aataaaccca
     1261 cgccgagtcg ctcgaagaca acgcattatt ttgcgcggca tgccgtacgt aaaaacgcca
     1321 taaaaaagcc accttcgtca ggtggctttt ttaaatatcc caatgatcag gttcaattag
     1381 ttcttgtttc tggccgtcgt attctcagta gctacaaatt cgaaggactt cagaaacttt
     1441 ccaatgttgg catccttttc tttgtagaag gctccaaaca catacatccg acggtcatca
     1501 ccgaccagct tagtaaaaac tcttgcgact ccaggaagag tgcagccctg aaaattaaat
     1561 tcttgtcccg tgtagccttt catcgaaaca gttctcggct caggagtctc gcattgaaag
     1621 gagcctgcac catttcgcgc ttcaaagctg cttgccattc ctcggaggaa tccctgcatc
     1681 gttccgttga tagcatcggc gtcagactct cccatgcttg gaccagttac ccacatcacc
     1741 tgatagactg agaggttctg gcggcccata tagatgtgaa agttaattag ccgatcgcca
     1801 gacgggactg gcaggcttat cgatttaccg tccaccggaa ccaggactga aaacttctca
     1861 ccagccggtc gaagtgtgcg ccactgtcca ttcacttctg gcgcataatt ttccccggca
     1921 tcttttgaat tgtctgaagg cggcctgctc gcggcaatgt tggcccaccg caatccttca
     1981 gtagtcaaac ctgaattagc actctttctt ggagctgaca aaggtgtcgc cggtgcagat
     2041 gcagaagaaa cttgcgcttt gctggctttc acaaagtccc agggcgccac aggattatca
     2101 acttgccata atccccttct ctcgtttcgc gcagcaattt gcgactgtgt gtagatctcg
     2161 ccatccgctg gagtcagaac aggactctta ggatcaaacc aggcagcacc atcccgaatc
     2221 atctgcgcac aaatatcgcg gtctcccacc aggacccgcg caatcaatgc ataattcttc
     2281 gtatagccgg cagattggac caaaacaatc ttgtccatta ccagatctga caaatgttgt
     2341 tttgcgacgt cgccgaaggg ttgcgtcaac tcaggagcgt ctacacccaa cagccggaca
     2401 cgaaccggcc ggttttgatt tgacaccgtt atgacgtcac cttcgttgac ttcgatcact
     2461 tttcctaaga acgccgcacc gtttacccgg ggcacgacta taagaaggct ggcgaatgcg
     2521 atagcggtcc aaagagtccg tggtatcttc atttcttcat tgctttccca atttggtatt
     2581 ccggtccagt tagaccgcaa attccttcgc aagcttcaac tcggccttgc ggagttcgta
     2641 tctggcgcga ccgagattgc ccttagcttc aagcgccagg acaacaaagt agtcgcggct
     2701 taacatcctc atgatcagcg tcgctccttc gagtgaagct accagctcct tcaatccacc
     2761 cagaccagta tcatgggccg ctcgttgagc atttctgatt agagaagtaa attccgctgc
     2821 tgctacatca aggttggcgt cgaggcctgc ctcggccaac actttttcaa cagcaattcc
     2881 gtctgtaccc ataatgagcg caccgagacc tccctcggtg cgctcaataa cggtttgcaa
     2941 catttccttg aacatcgatt
//
LOCUS       DASSFP010000167         5374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2125557, whole genome shotgun sequence.
ACCESSION   DASSFP010000167 DASSFP010000000
VERSION     DASSFP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125557"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFH91_03610"
     CDS             <1..279
                     /locus_tag="VFH91_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862100.1"
                     /translation="DKTYPSMEQANEFLKVVNDPATGKFYVHCAGGRHRTGMMGAVYR
                     FNHDRWSFDQAYAEMKQYDFYTSNGHDKQLDFIKDYWQQFQTKQASKS"
     gene            complement(552..1004)
                     /locus_tag="VFH91_03615"
     CDS             complement(552..1004)
                     /locus_tag="VFH91_03615"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET6862101.1"
                     /translation="MTERYLEDFSVGQVFRAGPLRVNSDRIKSFASEFDPQPFHLDEN
                     AASETMFGGLAASGWHTAAMTMRMLVDSDLKPAGGIIGVGFEEFRWPAPVRPDDELRI
                     EIEVLDIKTSRSRPTQGFIKLRTTTLNQDNTAVQIAVGQLLVPRRRQQ"
     gene            1123..1557
                     /locus_tag="VFH91_03620"
     CDS             1123..1557
                     /locus_tag="VFH91_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6862102.1"
                     /translation="MKNKKLLRIRPTIRLTLVLVLTVISSSTAALAVRQYKVATLPER
                     TLRKWAKITVMPVFPTESKNRKSQGVAVAEVQIDELGNVSKVEVLEAPDPPIAKALIV
                     AIKQWKFEPGKDDSDGKPIRMQGKLTFYFVIEGQTAQVKNPN"
     gene            complement(1612..2202)
                     /gene="def"
                     /locus_tag="VFH91_03625"
     CDS             complement(1612..2202)
                     /gene="def"
                     /locus_tag="VFH91_03625"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6862103.1"
                     /translation="MKLKIVQAGDPVLRVASRPLTVEEIKSASVQQLIELMRETMRDA
                     PGVGLAAPQIGVGIQLAVIEDREEYQDPASADQLSERHRSPVDFHVIINPTIKISGDS
                     SVEFFEGCLSVAGFAGLVRRGLRVTVNCLNERAEPVTIEAEGWYARILQHEIDHLKGT
                     LYIDRMESRSFTTVENMSRVWNGLTIEQVRTFLNLS"
     gene            complement(2312..4003)
                     /locus_tag="VFH91_03630"
     CDS             complement(2312..4003)
                     /locus_tag="VFH91_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET6862104.1"
                     /translation="MTGNWIPKSVLCLKAGYTRRDLIADLIAGVTVGLVALPLAMAFG
                     IASGVTPQAGIYTAIVGGFLISALGGSRLQIGGPTGAFVVIVGGIIAAHGTSGLLMVT
                     LMAGVLLIVMGVTGLGTAVKYIPRPVTIGFTNGIAVLIASTQIGDFLGLSMGAVPSAF
                     LLRMSFLAANLGSIHWPALAIAFSSLTLILLVPRLTKRVPGSIVALVLATLCVIAFGL
                     PVETIGSKFGGIPTGLPHVSLPHLRSDLILPLLPAAVTVAMLAAIESLLSAVVADGMS
                     GGDRHNSNVELCAQGIANVAVPFVGGIPVTGAIARTATNIRSGARTPISGIIHALTLL
                     LILLIAAPLARFIPLGTLAAILFVVAYNMSEWREVRTILKLSNADRTVWLMTCALTVL
                     ADLTVAVEIGMLLAALLYIYRVSQTTTVAPVTEEYIEDGRPHVLQDKLLPPYVLVLRI
                     HGPFLFGATEKLSEATIEIDSLPPVVILRLRNMTAIDATGLHELEKLNDRLRATGRML
                     LLCGARKQPEQLLRQSTFLRHLGEENMLPHVEAALSRAREVYEKQAGQFATLVSR"
     gene            complement(4060..4506)
                     /locus_tag="VFH91_03635"
     CDS             complement(4060..4506)
                     /locus_tag="VFH91_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET6862105.1"
                     /translation="MTSSRTTHSKKWARGLYVRESPLEGFGCFATIRFPKASPIAEYV
                     GEKITHSEAMRRLGGSGGKCISELDSDCYIDGSVGGNYTQFINHSCEPNADARVADGF
                     MIIVAIRDILPGEEVTVDYLNSFAEDLAVCQCGTPSCRQKIKRRAA"
     gene            complement(4539..4910)
                     /locus_tag="VFH91_03640"
     CDS             complement(4539..4910)
                     /locus_tag="VFH91_03640"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6862106.1"
                     /translation="MRQQLSDFKAEFFKALAHPLRISILDALRERELTVNEISEHFGV
                     EPANASQQLAVLRNRNIVVTRKEGANVYYSVSDKSIFKLLDAAKEIFNNHLVGVRSML
                     EEIHEDSPAKPARRSRAQRAR"
     gene            complement(5132..>5374)
                     /locus_tag="VFH91_03645"
     CDS             complement(5132..>5374)
                     /locus_tag="VFH91_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6862107.1"
                     /translation="VGTRLVTEVCKWAWKRGYRAVTLTTFRDVPWNMPFYSRLGFEEI
                     PKRELTPGLRAVLCSEARRGLDPSRRVAMRWWVPFR"
BASE COUNT         1375 a         1401 c         1356 g         1242 t
ORIGIN      
        1 gataagactt atcccagcat ggaacaagcc aacgagtttc taaaggttgt taatgatcca
       61 gcaaccggta agttctatgt tcactgcgct gggggtcgtc atcgcacagg catgatggga
      121 gcggtgtacc gattcaacca cgatcggtgg agttttgatc aggcctacgc tgagatgaag
      181 caatacgatt tctacaccag caatggtcat gacaagcagc tagactttat caaagattac
      241 tggcagcaat tccaaactaa gcaagcaagc aaatcctagc gtgagcgcat cggccaataa
      301 ataatacggt gtcctttaac agggcggtct caatggatgg ccccattcta tcttcacccg
      361 gcaactctct ccaggaagat cccgattaca acttttcacg gatgggtgac gctcgtcagc
      421 cccttggggc ttaagcagcc gcacgcttca aagcaggggt tagcatggcg ttgctcctgg
      481 atgagggttg ggtttctcaa aggggcaatc tcgagatact ccgccctcat cctcagcaag
      541 ttttgaatca atcattgttg acggcggcga ggaacaagta attggccgac ggcgatttga
      601 acggcagtgt tgtcctggtt cagagtcgtt gtccgcagct tgataaatcc ttgagtggga
      661 cgtgatcgcg aggttttgat gtcaaggact tcgatttcga ttcgcaactc gtcgtccggt
      721 cggacgggtg cgggccagcg aaattcttca aagccaaccc cgatgattcc accggcgggt
      781 ttgaggtcgc tatcgaccaa catgcgcatt gtcattgctg cagtatgcca accgctggca
      841 gcgagaccac caaacatggt ttcacttgca gcattctcat cgagatgaaa cggctgcgga
      901 tcgaactccg acgcaaagct ctttatgcga tcgctattca ctcgcaatgg tccagcgcga
      961 aaaacttgac caacgctaaa gtcttccagg tatcgttccg tcatttttaa ccctcaccgc
     1021 gaaaatattt gtgaaacaaa tgctaccaaa agaatcgatt gacatttaat atttcgcgaa
     1081 tttaaattac gcacgctcaa actggagaga aatcagaaga tcatgaaaaa caagaagttg
     1141 ttgagaatta gacctactat caggctcact ttggtgctgg tccttacagt gatcagctca
     1201 tcaaccgccg cgctggctgt gcgtcaatat aaggttgcga cgctgccaga gcgaacgcta
     1261 cgaaagtggg cgaagattac agtaatgcca gtcttcccga cagaatcgaa aaaccggaag
     1321 tcacaaggag ttgcagttgc ggaagtgcag atcgatgaac tcggaaacgt gagcaaggtt
     1381 gaagtcctgg aagcacccga tcctcctatc gctaaggctt taatcgtggc catcaagcaa
     1441 tggaagttcg agcccgggaa agatgacagt gacggtaagc ctattcggat gcaagggaag
     1501 ttaacctttt acttcgttat tgagggtcaa acagctcaag ttaaaaatcc aaattaatca
     1561 gattgtaaaa gtgaatggga tgaatcctaa caagacaatt tgagtcttca attagctcag
     1621 gttcaggaaa gtgcgaacct gctcgatcgt caggccattc cagacccgac tcatattctc
     1681 aacggtggta aacgaacgac tctccatccg atctatatac aaagtcccct tgaggtggtc
     1741 tatctcgtgc tgcagtatcc gagcatacca accttcggct tcaatggtaa ctggttcagc
     1801 acgttcgtta aggcagttga ctgtcacgcg aagcccacga cggaccaatc ccgcaaaacc
     1861 ggccacactt agacaaccct caaagaactc aactgatgaa tcgccggaaa ttttgatcgt
     1921 cggattgatg atcacgtgaa agtccactgg agatctgtga cgttccgaga gttggtcagc
     1981 ggaagccgga tcctgatatt cctcacgatc ctcaatgacg gcgagttgta tccccacgcc
     2041 gatttgagga gccgcaaggc caacccctgg tgcatcccgc atcgtctcgc gcatcagttc
     2101 gattagctgt tgcaccgaag cacttttgat ttcttccacc gtgagcgggc gactggcgac
     2161 tctcaagact ggatcgcctg cttgaacaat ctttagtttc atttaaggaa tgatgacatg
     2221 cttcagtgag aatgataaag cggagagcct ggatattgcc gctggccttg tcaagatttc
     2281 cccgtgagtc gtcgagatga taagccatgt tttaacgtga aacgagagtg gcgaactggc
     2341 ctgcctgttt ctcatagact tcgcgggcgc gagataacgc cgcttcaaca tgaggaagca
     2401 tgttctcctc gccgagatgc cgcaggaatg tcgactgcct caacaattgt tccggctgct
     2461 tgcgggcacc acacaacagg agcattcgac ctgtcgctcg caggcgatcg ttcagcttct
     2521 ccagctcatg caaacccgta gcgtcgatgg ctgtcatatt tctaagacgc aagatcacta
     2581 ccggtgggag cgagtctatc tcgattgttg cctcggacag cttctctgtt gccccaaata
     2641 agaaaggtcc atgtatccga aggacaagaa cgtagggagg caagagcttg tcttgtagaa
     2701 cgtgagggcg gccatcctca atgtactcct ccgtcacagg agccaccgtc gttgtctgcg
     2761 agacgcggta aatgtacagg agtgcagcca atagcattcc aatctcaact gcaacagtaa
     2821 ggtcggccag cacagtgagg gcacaggtca tcaaccatac ggttctatcc gcattcgaga
     2881 gcttgagtat tgtcctaact tcgcgccact cgctcatgtt gtacgccacc acgaaaagga
     2941 tggctgcaag cgttcccaat ggaataaaac gagccagcgg tgcagcgatc agcaggataa
     3001 gaagcaaagt tagtgcgtgg atgattccag aaataggagt gcgagcgccg gatctgatat
     3061 tggttgcggt gcgagcgatg gccccggtca ccggaatacc tcctacgaaa gggactgcga
     3121 cattggcaat gccctgggca cataactcca cgtttgagtt gtgtctgtca ccaccgctca
     3181 tgccatcggc aaccacagca gataataggc tttcaatcgc tgctaacatg gctaccgtga
     3241 ccgctgccgg cagcagaggc aatatcaaat cgcttcgcag atgtggtaag gaaacgtgag
     3301 gaaggccggt agggatacca ccaaacttac taccgatggt ttcgacgggc agtccaaacg
     3361 caattacaca gagggtggcc aggaccagcg caacgatgga accgggtacg cgcttcgtca
     3421 accttgggac cagtagaatc agagttaagg aactgaaggc aattgccagt gcgggccagt
     3481 gaatgctgcc gaggttagcg gcgaggaagc tcattcgcaa caagaacgcg ctcggtaccg
     3541 ctcccattga caggcccaga aagtcgccaa tctgtgtgga ggcaatcagg acggctatgc
     3601 cgtttgtgaa gccaattgtc accgggcgcg ggatgtattt cactgcagta cccagtcccg
     3661 ttactcccat gacaataagg agcaccccgg ccattaatgt gaccatcaac aaacccgaag
     3721 ttccatgggc cgcgataatc ccgccaacga tcacgacgaa agcgccggtt ggaccaccaa
     3781 tctgaagacg tgagcctccc aaagcagaaa tcagaaagcc gcctacgatt gccgtgtaaa
     3841 tgcccgcttg cggtgtgacg ccagaagcaa ttccaaaggc catcgctaag gggagtgcaa
     3901 cgaggccgac agtcacacct gcaatcaagt ctgctataag atcccgacgc gtgtagcccg
     3961 ccttcaggca caaaaccgat ttggggatcc agtttccagt caacaaagtc attgttcttg
     4021 ttagtgaggg agaaaggtgt gccgatcctc ctcaagacct tatgcggctc tacgtttgat
     4081 cttttgtcga catgagggag tgccgcattg acagaccgcc aggtcctctg caaaagagtt
     4141 caagtagtcg accgtaacct cttctcctgg cagtatgtct cgaatggcaa cgatgatcat
     4201 aaagccgtca gcaaccctgg cgtcggcatt aggttcacag gaatgattga taaattgcgt
     4261 atagttccct ccgacactcc cgtcgatata acagtcagaa tccaattcgc taatgcactt
     4321 tccacccgat cctcccaggc gtcgcatggc ctcgctgtga gtaattttct caccgacgta
     4381 ttctgctatt ggactggcct tcggaaagcg aatggttgcg aaacaaccaa agccctcgag
     4441 cggtgattct ctaacgtaca aaccccgcgc ccacttcttg ctatgcgttg tgcgggaact
     4501 cgtcatcctt ttggaaggtg tatgaagcgt aagtctgtct aacgggcccg ttgagcccgt
     4561 gatcggcggg ccggcttagc cggcgaatct tcgtggatct cttcaagcat gctgcggact
     4621 ccaacaaggt ggttgttgaa gatctcttta gccgcatcaa gcaacttgaa tatgctcttg
     4681 tcgctcacag agtagtagac gttcgcgcct tctttgcggg tgacgacgat gtttctgttc
     4741 cgtagcaccg caagttgttg cgaagcgttg gcgggctcaa caccaaaatg ttcactgatt
     4801 tcgttgaccg ttagttcacg ctcccgcaaa gcatcaagaa tagatattct caacggatga
     4861 gccagagctt tgaagaactc cgctttgaag tcgctcagtt gttgtcgcat tcaactgttt
     4921 ccgcatttct tccgattcat aagtaaaata gctgtctgtt ttacggcaga cctgcgcatc
     4981 tgcagattct tgcatatgct aaggtacacg attgtacttg tcaacttatt atgtgaggca
     5041 aggtgccagt ccagtgctga ttaggatacc cacaccacga ttttgatcgg tggtccaatt
     5101 accgtttttc tttaccagtg gtacccacct ctcaacgaaa aggaacccac catctcatag
     5161 caactcttcg cgacggatcg agtccgcggc gggcttcgct acagagaaca gccctcaacc
     5221 ctggggtgag ttccctcttg ggaatttcct caaaacccag gcgtgaatag aatggcatat
     5281 tccagggcac gtcgcgaaaa gtagtcagcg tcactgctcg atatccacgc ttccacgccc
     5341 acttgcagac ctcagtcacg agtctcgtac ccac
//
LOCUS       DASSFP010000168         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1373385, whole genome shotgun sequence.
ACCESSION   DASSFP010000168 DASSFP010000000
VERSION     DASSFP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373385"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="VFH91_03650"
     CDS             complement(<1..1679)
                     /locus_tag="VFH91_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET6862108.1"
                     /translation="MPAQPNVRQNTKRGLAYCLLAFFIFPITPCESHAQGTGNQNNSV
                     STERHKYSIDLKLDFDSLSYSGSERVQWFNRGDKPSSLVYFHLYPNLRAGEPVNTSNA
                     PSDLDEPRLDIVEVKTGNRETALPFSLEDQGATLRVNLREPVAPDESTEIFLKFKGTV
                     PEIDPDETSLTTHVVKQVSAALRSERELRRARDVNFRCRGLMLLASAYPVLAVHDGDE
                     WRRRVEPSVGDFVFNEISDYEVTITSTPGVEVFTSAAEKSADAEKSTQTFVASTLRDF
                     AILAGKGLRSEQVDVEGIRVRSIYLSEHERVGKRALTTAAAAVRVFTSVFGPLPFKTI
                     SVAEAPLVAGLGSTEFSGLTIIASAFFVDFDSPAMRNLPEIIREQRPSVEESLEWTVA
                     HLVAHQWWGAAVGNDPAKEPVLDEALSCWSSLVYYKSTYGEERAAAVLEDQVKGVYRL
                     YRTFGGDDMDADRPARDYRNTFQYAAIVTAKGALMFVELQRSLGDEKIYAALRNYYKA
                     NLFEIAQLDDLRSALVAEAPIEQRRLIARTFTRWLTSRRGDEDISPPDQDLA"
     gene            complement(1693..>2946)
                     /locus_tag="VFH91_03655"
     CDS             complement(1693..>2946)
                     /locus_tag="VFH91_03655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6862109.1"
                     /translation="GQASNEVLETATTLGRLLPLKDHRPAIECLQQAGDRLGHTAPEI
                     EQALVRIGPDAYISSFGLDQAAKYTLVSSVLLNWRSASAYAAGLREFAALPDSFQNKP
                     QLSKAAESFLRDMLAYKTSGIRINTLVAVHSEYAVPDILRALAVYKPADMGEILRSQL
                     KESDPIIRATAADLLGELPASEINTQALATALPVALKDQLNDAMLSTLDALGIQKNEA
                     ANSVIKSALESPDYLVRQRAASLLKANKVGDFDSKIGTVQTRNSDNDYRRAIARIGQS
                     VHAVVTTSKGSFTIELLPNDAPLNVDNFLTLAQKGYFRNITFHRVVPNFVIQGGDPRG
                     DGNGGPGYQIRCEINEVEYDRGAVGMALSGKDTGGSQWFVTHSPQPHLDGGYTVFGRV
                     ITGMNVVDNISRGDVIQSIRISTGR"
BASE COUNT          705 a          737 c          773 g          731 t
ORIGIN      
        1 gccaaatcct ggtcaggtgg cgatatatcc tcatcgcctc tgcgagaagt tagccagcgg
       61 gtgaatgtac gtgcaatcag ccgcctttgt tcgattggag cctcggcgac aagtgcactt
      121 ctcaaatcgt cgagctgagc aatttcgaag agattagctt tgtaatagtt tcgcaaggcg
      181 gcgtaaatct tttcgtcgcc cagggaacgt tggagctcga caaacatcaa agcgcccttg
      241 gccgtgacta tcgctgcgta ttgaaatgtg ttccgatagt cgcgtgcggg acgatcggca
      301 tccatatcgt ctccgccgaa cgtgcgatag agccggtaaa ctcctttgac ttgatcctca
      361 agaactgcag cagctctttc ctcgccataa gtgcttttgt agtagacgag tgacgaccaa
      421 catgacagag cttcgtctaa tacaggttcc ttcgcaggat cattcccaac cgcagcaccc
      481 caccattgat gtgcgacgag gtgagcaact gtccactcca ggctttcctc gaccgacggc
      541 cgttgttcgc gaatgatctc gggtaagttg cgcatggccg gtgagtcaaa gtcgacaaaa
      601 aaagcgctgg cgataatcgt tagacctgag aactcagtgc ttccgagtcc tgcgaccaga
      661 ggagcttcag cgacgctgat ggttttgaaa ggaagcgggc cgaacactga tgtaaaaact
      721 cgcactgccg cagctgcggt cgtaagggca cgttttccaa cccgctcgtg ttcagacaaa
      781 tagattgacc tgactctgat gccctcaaca tcgacttgtt cactgcggag tcccttaccg
      841 gccaggattg cgaaatcgcg aagcgttgaa gcaacaaacg tttgagtaga cttttccgcg
      901 tcggcgcttt tctcggcggc tgaagtaaag acttcaactc ccggtgtact tgtaatggtg
      961 acctcgtaat ccgaaatttc attgaacacg aaatcaccaa ccgaaggttc tacgcgtcgc
     1021 cgccattcgt ctccgtcatg tacagccaga accggatacg cggaggcgag tagcataagg
     1081 ccgcggcacc gaaaattgac gtcccgagcg cgtctcagct cacgttcact ccgcaaggct
     1141 gcacttactt gcttaaccac atgcgttgtt agactagttt cgtcgggatc gatctcggga
     1201 acagtgccct taaatttaag gaagatttca gtactttcgt ctggcgctac aggttcgcgc
     1261 aggttcactc gcaacgttgc gccctggtcc tcaagggaaa aaggaagagc tgtctcgcga
     1321 ttaccggttt taacctcaac tatgtccagt cgcggttcat ccagatcgga aggtgcattt
     1381 gaggtattaa cgggctcccc cgcacgtaag ttgggataaa gatggaagta gaccaatgac
     1441 gaaggcttat ctccgcgatt gaaccattga acacgttctg atccactgta ggaaagactg
     1501 tcaaaatcga gttttaaatc gatcgagtat ttatgacgtt cggtggaaac tgaattgttt
     1561 tgattaccag ttccctgtgc gtgggattcg cagggagtaa ttggaaatat gaagaacgcg
     1621 agcaggcaat aagccagtcc tcttttagtg ttttgtctga cgtttggttg tgcgggcatc
     1681 aataaactgt ccttaacgtc cagtactaat tctgatcgac tggattacgt caccgcgtga
     1741 aatgttatcg acgacgttca tacctgttat gactcgacca aaaacggtat atccaccgtc
     1801 tagatgcggt tgtggtgagt gggtaacaaa ccactgtgac ccgccagtat cctttcctga
     1861 gagcgccatg cctaccgcac cacggtcgta ttcgacctcg ttgatttcgc agcgaatctg
     1921 atagccaggt ccgccattgc cgtcaccacg agggtctcct ccctgtataa caaagttagg
     1981 aacaacccga tggaaagtaa tgtttcgaaa ataacccttt tgcgcgagcg tcagaaaatt
     2041 atcaacattg agaggggcat cattgggaag cagttcaatc gtaaacgaac ctttgcttgt
     2101 agtcactacc gcgtggacac tttggccgat tcgagcaatt gcgcgtctgt agtcgttgtc
     2161 ggaattgcgg gtctgaaccg taccaatctt gctatcgaag tcaccaactt tgttggcctt
     2221 gagtagggaa gctgcgcgct gtcgcaccag atagtcggga gattccagtg ccgatttgat
     2281 aacggaattt gcggcctcgt ttttttgaat tccaagggca tcgagcgtag atagcattgc
     2341 gtcatttagc tgatctttca gagccaccgg taaagcagtt gccaatgcct gagtgttgat
     2401 ttccgaggct ggtaattctc caagcagatc tgccgcagtg gcgcgaataa tcggatcact
     2461 ttctttgagt tgactgcgaa gtatctcacc catgtcggcg ggcttgtaga cggcgagagc
     2521 tctgaggata tcgggaacag catattcgga atggaccgca acaagagtgt tgattcgtat
     2581 cccagaggtc ttataggcga gcatgtcgcg caggaatgat tcagccgcct tcgacaactg
     2641 tggtttgttc tgaaaggaat cgggtaatgc cgcgaattct cttaagcccg ctgcataggc
     2701 tgaggctgag cgccaattta gcaagacgga cgataccagg gtgtacttag cggcctggtc
     2761 tagtccgaac gagctgatat aagcgtctgg cccgattcgg acgagagcct gctcaatttc
     2821 aggcgccgta tgtccaagtc gatccccggc ctgttgaaga cactcgattg ccggccgatg
     2881 atctttgaga ggaagtaagc ggccaagtgt tgtcgcggtc tccagtactt cgttcgaagc
     2941 ctgacc
//
LOCUS       DASSFP010000169         4043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_490053, whole genome shotgun sequence.
ACCESSION   DASSFP010000169 DASSFP010000000
VERSION     DASSFP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490053"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFH91_03660"
     CDS             complement(<1..974)
                     /locus_tag="VFH91_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862110.1"
                     /translation="MRSIYPFSFILPLLLVIMIPANSQTNARMQKSNLITIRLNSGWQ
                     FREASKDSWYPATVPGSVHTDLMKNQLIGDSFYRDNEQKQQWIGKTDWEYRSTFRVSQ
                     EVLNHENLELVFDGLDTYADVFLNDVPVLKADNMFRTWRVNCANRLKPGDNTLRIYFR
                     SPINEVLPLMAKMSYQLPASNDQGEKTSPHTRKAPYQFGWDWGPRLVTSGIWKSISIE
                     AWDKARISDLHIAQNLVSKESAGITANVTIEASGDAEAHLIIEDVTNKIIRAKKEVKL
                     SQGTNKFPLEFEILRPALWWPNGLGPHPLYNLKARLLIGRKLLDERST"
     gene            1247..2188
                     /locus_tag="VFH91_03665"
     CDS             1247..2188
                     /locus_tag="VFH91_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6862111.1"
                     /translation="MLKLHRLSFCAILLLAIFGEVYSQQSVKPLVERLGYPANTKLLI
                     VHADDLGMAHSINDATVKAFATGLVNSGSIMIPCSWVPEIAEYARKNPQADLGLHLTL
                     TSEWKQYRWGPLSDKTRVPSLVDSNGYLFPTESEASARAKVEEVEFEIRAQIEQAKKL
                     GIIATHLDSHMGTLYQSKELFETFLKVARDNKLPVRMSKEWFSRMPFLPSLLKPEDVI
                     LDHIITIEPDITADGWAKFYADALKQLQPGVTEFVIHIAHDDEEMQGVSFEHPNWGAA
                     WRQRDFDFFTSDAFRKLLDDNQIKLITWRDLQKLISK"
     gene            2217..>4043
                     /locus_tag="VFH91_03670"
     CDS             2217..>4043
                     /locus_tag="VFH91_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HET6862112.1"
                     /translation="MTPQKPVRFAVLLGLVLLSSTLVAQQTPSDSAMTYQHNLMPVPA
                     SVQFGSGKLTINGGFRVAMNQSDPRVQAALERFLKRFQGRTGLTFAASVSNDVAGSAL
                     VVQFQGPGKSIPSLDEDESYKLEVTADKAQLSAPTAVGAIRGLETVLQLLSSDRDGYF
                     LPVVTVQDQPRFRWRGLMIDIGRHYEPPDVLKRNLDAMAAVKLNVFHWHLTEDQGFRI
                     ESKKFPKLHEMGSDGLFYTQEQARDIIEYARQRGIRVVPEFDIPGHSTSWLVGHPELG
                     SASGPYSIERRAGIFEPALDPTREEVYKFLDTFLGEMAALFPDEYLHIGGDENEGKQW
                     DRNPKIQAFMKEKGIKNNHALQAYFNQRLLKILEKHKKKMIGWDEILQPEIPTSAVIH
                     SWRGTTALAEAVRKGYSGILSNGYYIDLSYPTSQHYLVDPIPADTTLTSEEQKRILGG
                     EATMWGEYVGPETIDSRIWPRTAAIAERLWSPQNTRDVDDMYRRLDVISMQLEELGLT
                     HERNVDVLLRRLAGQKEIGSLKLLVSLVEPVKEYRRGQQRPSTMLSPLTGFVDAARPD
                     SEPARKVARMVDALLSDAPKFQPYASDLKSRFSQWSDAEAELN"
BASE COUNT         1011 a         1015 c         1019 g          998 t
ORIGIN      
        1 ttcgtcgaac gctcgtctaa aagtttcctt ccaattaaca aacgcgcttt aaggttatac
       61 aacggatgtg ggcccaatcc gttgggccac caaagcgccg gacgcaagat ttcaaactcg
      121 agtggaaact tgttcgttcc ctgacttagc ttaacttcct tctttgctct tattatcttg
      181 ttggtaacat cttcaataat caggtgcgcc tcagcatctc ctgaagcttc aatcgtgaca
      241 ttcgcagtaa tgccggcact ttctttactt acaagattct gcgcaatgtg gagatccgaa
      301 attctcgctt tgtcccaggc ttcgattgaa attgatttcc agattccact ggtcaccagt
      361 cgaggacccc agtcccaccc aaactgatac ggagccttgc gcgtgtgtgg tgacgtcttt
      421 tcgccctgat cgttggacgc ggggagctgg taggacatct tggccattaa cggcaagact
      481 tcgttaatcg gtgagcgaaa gtagatccga agagtgttat cgccgggttt cagccgattc
      541 gcgcaattca ctctccaggt ccgaaacata ttgtcggctt tgagaactgg tacatcgttc
      601 agaaagacgt cggcgtaagt atccagtccg tcgaacacta actcgagatt ttcatgattg
      661 agtacttcct gagacacccg gaaggtcgag cgatattccc agtctgtttt accgatccat
      721 tgctgctttt gctcgttgtc gcgataaaat gaatcgccaa tgagttgatt cttcatcaag
      781 tcagtatgga cagatcctgg tactgttgcg ggataccaac tgtctttgga cgcttcccga
      841 aattgccaac cactattgag tcgaattgta attaaattcg atttctgcat gcgcgcgttc
      901 gtctgagagt tcgcgggaat catgattacc agcagaagtg gcagtatgaa tgaaaagggg
      961 tagattgatc tcaagcttga cctcgcacat ttttgaaagc gtctggtgtt tcggcaaacg
     1021 taatacttga ttttttcttt actcgcctga taaaaggagc gcgtctctcg aacgcacttt
     1081 aaatgctggt gggcggatga tagtcatttc attacggtac atgtcaacta tcttgggcta
     1141 gtcacgacca acaaggtgct gctaatgttt cagcagtcac cggcatcttt cttgcgcata
     1201 ttaagcactc gcgcacctgg aatacgctca agctttgagg attctgatgc tcaaactaca
     1261 tcgactctcg ttctgcgcaa tactgctgct cgcgattttt ggtgaggttt acagtcagca
     1321 gtccgtaaaa cctctcgtcg agcgattggg ttatcccgcg aacacaaaat tactaattgt
     1381 tcacgccgac gacctgggaa tggcgcactc aatcaatgac gcgacggtaa aagcgtttgc
     1441 gacaggactt gttaattcgg gcagcatcat gatcccgtgc tcctgggtcc cagagatcgc
     1501 tgaatacgct cgtaaaaatc cgcaggcgga tctcggcctt caccttacgc tgaccagtga
     1561 atggaaacag tatcgctggg gaccgttgtc agacaaaact cgagttccgt ctctggttga
     1621 tagcaacggt tatctttttc cgaccgaaag tgaggcttcc gctcgtgcaa aggtcgaaga
     1681 agtggagttt gaaatcagag cccagatcga acaagcgaaa aagcttggga taatcgcaac
     1741 gcacctcgat tcccacatgg gaactcttta tcaaagcaaa gaactatttg aaacgttttt
     1801 gaaggttgcg cgagataata aacttcctgt caggatgtcg aaagaatggt ttagcagaat
     1861 gccgttcctg ccgtcacttc tgaaaccgga agatgtaatt ctggaccaca ttatcaccat
     1921 cgaaccagat ataaccgcag atgggtgggc caagttctat gcggatgcat tgaaacaact
     1981 acaacccgga gttactgagt ttgtgatcca tatcgcgcat gatgatgaag agatgcaagg
     2041 agtctcgttt gagcatccga actggggcgc tgcgtggcgc cagagagatt tcgacttttt
     2101 cacgagcgac gcgtttcgca aattactcga cgacaatcag atcaaattga tcacctggcg
     2161 tgacctccag aaactgatca gcaaataaac ctcggtcgct tacttacttt caacaaatga
     2221 cacctcaaaa acctgttcgc ttcgccgttc tgctgggcct ggtacttctg tcttccaccc
     2281 ttgttgctca gcaaacacct tccgattcgg caatgactta ccaacacaac ttgatgcccg
     2341 tcccggcgtc cgttcaattc ggatccggaa agctcaccat taatggtggt tttcgggtgg
     2401 ccatgaacca gtccgacccg cgagtgcagg ctgcgctgga aagatttctg aaacggtttc
     2461 agggacgtac cgggctcacc tttgcggcaa gcgtgtcaaa cgatgttgcc ggatcagccc
     2521 tggtggtcca attccaggga cccggaaaat ccattccgtc cctcgatgaa gatgagagct
     2581 acaaactcga agtcactgct gacaaggctc agcttagcgc tcccacagcg gtcggtgcga
     2641 tccgcggcct tgagactgtg ctgcagctcc tctcttcgga ccgcgatggt tactttttgc
     2701 ccgtcgtcac cgttcaggat cagcctcggt ttcgttggcg tggtctcatg atcgacatag
     2761 gtcggcacta cgaaccgccc gacgtcctga aacgaaatct cgatgcgatg gctgccgtca
     2821 aacttaacgt ttttcattgg cacctgaccg aagaccaggg ttttcggatc gagagcaaaa
     2881 agtttccgaa gctacatgag atgggctccg acggactgtt ttacacacag gagcaggcaa
     2941 gggacatcat cgagtatgcg agacaaagag gcatccgcgt cgttcctgag tttgatattc
     3001 cgggccacag cacgagttgg ctggtcggtc atccagaact cggcagcgcg tctggtccat
     3061 actcaattga gcgtcgcgca ggtattttcg aaccagccct ggatccgaca cgtgaagagg
     3121 tctacaagtt tctcgacacc ttccttggtg aaatggccgc gctgtttcct gacgagtact
     3181 tgcacattgg tggcgacgaa aacgagggca agcagtggga tcgcaaccct aagattcagg
     3241 ccttcatgaa agagaaaggc atcaagaata atcacgcact ccaggcatac ttcaatcagc
     3301 gtctcctgaa aattctcgag aaacataaga aaaaaatgat cggctgggac gaaatactac
     3361 agccggagat tccaaccagc gccgtcatcc attcatggcg cgggacaaca gcactcgctg
     3421 aagccgttcg caagggctat agcggaattc tttctaacgg ttactacatc gatctcagtt
     3481 atccgacgtc gcaacactat ctggtcgatc cgattcctgc cgataccacg ttaactagtg
     3541 aagagcaaaa acggattctt ggcggcgaag cgacaatgtg gggcgagtat gttggtccag
     3601 aaacgatcga ctcacgaatt tggcctcgaa cagcggcaat agcggaacgg ctttggtcac
     3661 cgcagaacac aagagacgtc gacgatatgt accggcggtt ggatgtaatc agcatgcaac
     3721 tggaagagct gggattgacg catgaaagaa atgtcgacgt actgctgaga cgtttagctg
     3781 gacaaaagga aatcggcagc ttgaagctat tagtttctct ggttgagccg gtgaaagagt
     3841 atcggcgtgg tcagcagcgc ccgtcgacga tgctcagtcc gttgactggt ttcgtggatg
     3901 ctgcgcgacc agacagcgag ccggcacgaa aggtcgcgcg aatggttgac gctttactct
     3961 ctgatgcgcc gaagtttcag ccttacgcga gtgacttgaa gtcaaggttt tcgcaatgga
     4021 gcgacgccga agctgagcta aac
//
LOCUS       DASSFP010000170         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1530408, whole genome shotgun sequence.
ACCESSION   DASSFP010000170 DASSFP010000000
VERSION     DASSFP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530408"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFH91_03675"
     CDS             <1..612
                     /locus_tag="VFH91_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6862113.1"
                     /translation="FFDMYAVSTVLFRSTSWRKQLFFPVRGRFFYQHPLGLLVNLIMG
                     WWSMYPPFFSTGDHPIAEKRPFDKYSFRVLTELARVGAGNVIGFHPEGTRNKNDEPYS
                     FLRAQPGVGKLILDARPQVIPVFIAGLCNSLPRQIARNWNREELIRIHFGQQLDLAEY
                     LEQPDRLRTHKLIADHIMSKIAELGEQDRGLKASTKHDELHER"
     gene            622..1572
                     /locus_tag="VFH91_03680"
     CDS             622..1572
                     /locus_tag="VFH91_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6862114.1"
                     /translation="MPLPLVIVNPASASGTTNRIWPQLASDLRSGFGPFNVEFTDAPG
                     AAAAIAMRAAQKGTRLIIACGGDGTISEVANGILESGKDAELGILPSGTGGDFRRTLE
                     IAADSRSAARVLRDGKSRRIDVGRVTFTNNSGAEVIRYFLGVASCGMSTKVIEQVKSG
                     GPDWLPGSTSQWLSGRISFGASLMQTAMRSEPTRVVAQIDDEHERNLSISNLCIANAR
                     YFGGGMKIAPDAKLTDGKFDIISVGDLSALKIVTSAPRLYVGSHLSMPEVSHTLARRI
                     IIRSPMGGPQVDLEIDGELLGHLPATFQVVPRALMVRCNV"
     gene            1594..1953
                     /locus_tag="VFH91_03685"
     CDS             1594..1953
                     /locus_tag="VFH91_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862115.1"
                     /translation="MTGKEQKEQTVASLPVFLRRLLVPLGISFSLMGVALLIGISGYH
                     LIAGFNWADSLLEASMILAGMGPVNPLPTVSAKLFASAYALFSGVIFIGVIGIILGPF
                     VHRVLHKFHLEGGKSQK"
     gene            2073..2423
                     /locus_tag="VFH91_03690"
     CDS             2073..2423
                     /locus_tag="VFH91_03690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6862116.1"
                     /translation="MDERRSKPRLSVSLDAVWDGVEERHSARVTDLSEGGCYLDTVGE
                     VMTGEIVAFRVLLPDDDWLYLEGEVKHHRHGMGFGVQFVELNEEQTDKLLWLLRIAKE
                     AGPDTPISADLVED"
     gene            2613..3737
                     /locus_tag="VFH91_03695"
     CDS             2613..3737
                     /locus_tag="VFH91_03695"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET6862117.1"
                     /translation="MAIQGTLKTMNLTDLLQFLAAGRKTGTLKFDCGKITKQVYFKNG
                     LIVGSKSNDPKEYLGQTLLHYGKVTEEQLQAARETQRTTAGRLGEVLVQLGFLTEEDV
                     LAILKTKTLDAIYDLFMWTDGQFEFYDDEKLPDELILVEVEPTNVIMEGIYRIDELAR
                     YKTLVPNDRSILELAAGWTSSLKLGKEYRQIFFFIEKRMSVAEICYHMHASAFHVYGQ
                     LFDLIGKGVARVAGELPEVAQPPAAPIEDLPESTTEMLWAAERKVDKDPEAALSIIQR
                     VLQDEPNNAEAQKLLGAAEEKFIKNIFDTTKLSPQSIPKLLISPSNLTNTKMDPQEGF
                     VLSRINGSWDVQSILSICPFRDADCLRMIKDLIDRGIIGL"
     gene            3810..4226
                     /locus_tag="VFH91_03700"
     CDS             3810..4226
                     /locus_tag="VFH91_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET6862118.1"
                     /translation="MSENNKAILRRWFEEVWNRGQADVIDELLARDAVIHGLEDANGQ
                     PVESYAAYETFHRQFRGAFPNIAVSIDDMVAEGDKVAARCSVAAKHTGGTLGFEATHT
                     DVEFDGMTIVVIRDGKITEAWNNFDFMRMNKQLGLM"
BASE COUNT         1090 a         1016 c         1144 g         1078 t
ORIGIN      
        1 ttctttgaca tgtatgcggt ttctacggtc ctctttcgct cgacaagttg gcgtaaacaa
       61 ctcttttttc cagtgagggg ccgcttcttt tatcagcatc ctctgggtct gctcgtcaac
      121 ctgatcatgg gctggtggtc catgtaccct ccattctttt ccaccggcga tcaccctatt
      181 gctgagaaac gaccgttcga taaatactcg tttcgcgtct tgactgaact ggcgagagta
      241 ggcgccggca atgtgattgg cttccatcct gaaggaacgc gcaacaagaa cgatgagccg
      301 tactcgttcc ttcgagctca acctggagtc ggaaagctta tacttgatgc gcggcctcag
      361 gtgattccgg tctttattgc cgggttgtgt aacagtctac ctcgtcagat tgcgcgaaat
      421 tggaaccgtg aggaattgat tcgcattcat tttggacagc agctagacct tgcagagtat
      481 ctggaacaac ctgatcgttt acgcactcac aagttgattg ctgaccacat aatgtcgaag
      541 attgcagagc tgggtgaaca ggatcgcggc ctaaaagctt cgactaagca cgatgaactt
      601 cacgaacggt agaattgaaa tatgccatta cctttggtca tagtgaatcc ggcgtcagcc
      661 agcggtacaa cgaaccgaat atggccccag cttgcaagtg acctgcgaag tggttttggc
      721 ccattcaacg tagagttcac tgacgcgcca ggtgccgcag cagcgatagc catgcgtgcc
      781 gcgcaaaagg gaactcgctt aattattgcc tgcggcggtg atgggaccat ctctgaagtt
      841 gcgaatggca tcctcgaatc cggcaaagat gcggaactgg ggattctacc aagcggtacc
      901 ggcggagatt ttagacggac gcttgagatt gccgctgact ccagatctgc ggctcgcgta
      961 ctcagagatg gtaagagtcg gcggatcgac gttggccggg tgacatttac taataactcg
     1021 ggagctgaag tcatccgata tttcctcggt gtggcgtcgt gcggaatgag tacgaaggtt
     1081 attgagcagg tgaagagcgg cggacccgac tggcttcccg gtagcacttc ccaatggctc
     1141 agtggtcgta tctcatttgg agcatcgctt atgcaaacag ctatgcgttc agaaccaacc
     1201 cgggtcgtcg cacaaattga tgatgaacat gaaaggaatc tctcgatctc aaatctctgt
     1261 atcgccaatg ctcgatattt tggcggtggc atgaagatcg cgcccgatgc aaaactgacc
     1321 gatggcaagt ttgacataat aagcgttggt gatttaagcg cgttgaagat agtgacgagt
     1381 gcgccgcgcc tttacgtcgg ttcgcatctt tcaatgccgg aagttagtca cacgctggca
     1441 cgacgcatca tcatccgcag tcccatgggt ggaccacagg tggacctgga gatcgatgga
     1501 gagttgttgg gccacctgcc ggctaccttt caggtcgtgc cccgagcatt aatggtccgt
     1561 tgcaacgttt gagtaacgaa agtaaggaga attttgaccg gtaaagagca aaaggagcaa
     1621 acagttgcgt cgctgccagt gtttttacgg cgtcttctcg tcccacttgg tattagtttt
     1681 agcttgatgg gagtggcact ccttatcgga atttcggggt atcatctcat tgcaggtttt
     1741 aactgggccg attcactcct ggaagcgtcg atgattctcg caggaatggg acctgtcaat
     1801 ccgttaccga cggtgtcagc taagttattc gcttcagcct atgctttatt cagcggagta
     1861 atctttatcg gagttattgg tataatcctt gggccctttg ttcatcgcgt ccttcacaag
     1921 ttccacttag agggcgggaa gtctcagaag taaatcgcca gcggtgaatc ccctcccaaa
     1981 attcgttaca tgacgaaaat tgtctctttt tcgtcaggag taatttttct ttgcctgctt
     2041 ggacaaaaac cctatgatga gtgtgaggcc ccatggatga acgacgatct aaaccccgat
     2101 taagtgttag tctggacgca gtctgggatg gcgttgaaga acggcattcg gcgcgtgtga
     2161 cagacttatc tgaaggcggt tgttacctgg acaccgtagg cgaggtgatg accggagaaa
     2221 ttgtggcgtt ccgggtctta cttcccgacg acgactggct ctatttggaa ggtgaggtta
     2281 agcatcatcg acacgggatg gggtttggcg tccagtttgt tgagctcaac gaagagcaaa
     2341 ccgacaaatt gttgtggctt ttgagaattg ctaaagaagc tggaccagat actcctattt
     2401 cagcagatct ggtggaagat taagtgcgga gaaggtaacc ccggcacagt tatcgcaggc
     2461 tactccttcc gtattagacc aacaaccgaa ttgaaaatat tacaaacgct tgagaaagag
     2521 attactctcg tgcataggat cttgtcttct gcccagggga aacctcaact cctaatacga
     2581 ctgactgtga ggaacgatag ttcggtaaag taatggcgat tcagggcaca ttgaaaacaa
     2641 tgaacctcac cgatctgctc cagtttctgg cggcgggacg gaagacgggg acccttaagt
     2701 tcgattgcgg caagatcaca aagcaggtct acttcaaaaa tggtctgatt gtagggtcca
     2761 agtcgaacga ccctaaagag tatctgggcc aaactctcct gcattacgga aaggtaactg
     2821 aggagcaact gcaagccgct cgtgagaccc aaagaacgac cgcaggtaga ctcggtgaag
     2881 ttctggtaca gctcgggttt ctcactgaag aagatgtatt agcgatcctc aagactaaga
     2941 cactcgacgc tatctatgac ctgttcatgt ggaccgacgg gcagttcgag ttttacgacg
     3001 acgaaaaatt gccagacgaa ctcatcctcg tcgaagtgga acctaccaat gtgatcatgg
     3061 aaggcatcta ccgaatagat gaactcgctc gttataaaac tctagttcct aacgacagga
     3121 gcattctcga actggcagct ggctggactt catctctaaa gctcggaaag gagtatcgtc
     3181 aaatattctt cttcatcgag aagcgaatgt cagtagcgga gatttgttat cacatgcatg
     3241 catccgcatt tcacgtttat ggccagcttt tcgatttgat aggtaagggc gttgcgcgag
     3301 ttgccgggga attgccggaa gtggcccagc ctccggctgc tccaatcgaa gatcttccgg
     3361 agagcactac tgaaatgctt tgggctgcag agcgtaaggt cgacaaagat ccggaagcag
     3421 ccctgtcgat aatacaaaga gtcctgcaag atgaacccaa caatgctgaa gcgcaaaagt
     3481 tactcggcgc cgctgaggaa aagtttatta agaacatctt cgatacgacg aaattgtctc
     3541 cgcaatcgat acccaaactt ttgatttcac cttcgaatct tactaatacc aaaatggatc
     3601 ctcaggaagg atttgtgttg tctcggatca acggcagctg ggacgtccag tcgattctct
     3661 cgatttgtcc tttccgcgat gcggattgtc tacgaatgat caaagatctc attgaccgcg
     3721 gaatcatcgg gttatagagt ccatcccgaa agtccgacca ggggttgtta acgctgatag
     3781 cttcgacaac ttttctcacg gaggtcaata tgtcagaaaa caataaggcc attttgagac
     3841 gctggtttga ggaagtgtgg aacagaggcc aggccgacgt catagacgag ttgctcgcca
     3901 gagacgccgt tatccacggg cttgaggatg cgaatggtca gccagttgaa agttacgccg
     3961 cgtacgaaac ttttcaccgg cagtttcgcg gagcgtttcc gaatatcgct gtgtcgatcg
     4021 acgatatggt cgcagaagga gacaaggtgg cggcgcgctg cagcgttgca gccaaacata
     4081 ccggtggaac tctcggcttt gaggccacgc ataccgacgt cgaattcgat ggcatgacca
     4141 tcgttgtcat cagagacggt aaaatcacag aggcctggaa caactttgat tttatgagaa
     4201 tgaataaaca gctaggcttg atgtaggcag gagaaactaa gtgcgaataa cgctattgct
     4261 gttttgctgt ctcctgtcat tactttttga tgctcaacag cctcaacgcg gaacgctctg
     4321 cactgaaa
//
LOCUS       DASSFP010000171         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1680558, whole genome shotgun sequence.
ACCESSION   DASSFP010000171 DASSFP010000000
VERSION     DASSFP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680558"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFH91_03705"
     CDS             <1..757
                     /locus_tag="VFH91_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862119.1"
                     /translation="NAVWWILGGIAAFAIIGIGLLVMIIAIASMSGDTNNRNVSNINS
                     RTDNRNINVTANANVSENVNTSSLPAEFSDDFSQPKWGVGDSKFGRIWYADDEYHMSS
                     KERTYIVMYAPSDEYNTENATVKITARSVNGSVPSSGFGLMVHCVQSKAKQLEDYALL
                     LYPGETPQYEVIMHKDGNQSSLVSKTSSSAIRPGSNPNQLEVRIKGTEIAFYVNGQYL
                     TRITDTANYRRGRVGLYTSDVTDIAFDDLEIKR"
     gene            773..2080
                     /locus_tag="VFH91_03710"
     CDS             773..2080
                     /locus_tag="VFH91_03710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HET6862120.1"
                     /translation="MQVNLKVIAGPYMGRSFSFNQHDSFLIGRTPEAHLCLASDRYFS
                     RHHCLLEINPPHSFLRDLGSTNGTFVNGQRVHETSLRHGDRVQGGETILLVEVQASTS
                     DDLDETTRDALPPTRPVMVQVECLNCGRRDYAQASDENERLNFICEECRIELKNAPQG
                     VPGFDTIRILGRGGMGCVVLARQHQTGRDVAIKTLLPEFAVSDKALRRFMREMDVAAA
                     LKHKNIVEFIDRGTHNGVVYLVTEFVDGPDASRLAEVSGGRLSYPDAISVISQSLDAL
                     AYAHNQGYIHRDFKDQNILVAGKSPNLTAKLTDFGLAKSFTHSGMSGVTMAGEMAGTL
                     AYMPPEQLRNFRDVKPQSDIYAVGMTAYSLLTGTLALDLAKKTSINDTIRAIFEQPAV
                     PLRERANEIPQMVCDIIDRALAKDPTQRWQSATAMRNALLHAL"
     gene            complement(2088..3296)
                     /locus_tag="VFH91_03715"
     CDS             complement(2088..3296)
                     /locus_tag="VFH91_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HET6862121.1"
                     /translation="MKLSISLLLILITTLPFAGRDTDPDRWLRDAPGYEQALKLQQES
                     NEPLIVYFYTDWCPYCRSLDNQYLPSQPVRDYLRTVVKVRINPEHGRAERELAVRYGV
                     TGYPSYFVIQKASAAPRQISPFRRGAPNLTTAEFAVAMQSAVSMPARKVGTPNLVRDA
                     SSRAVVNATKQTTPSKILVAPAVVASLPSLDSILDRYVTVRGGRDAQSKLNSRVARGR
                     VDVVGVSFGGKLETYSKAPNKSLMIMQAEPIGLFKRGFDGRAGWDVSVKAGSQPIVGF
                     ELASLAVDSDFYRDLKLKQLYQSMKVVSRVHEGFRELFKVEATPRAGLPEYLYFDQET
                     GLLTRRETQRKTSQGMSLTEFYYSDWRDVDGVKIPFKTTQTIGKTTYVFSLEDVRHNV
                     PVDESMFNRP"
BASE COUNT          903 a          898 c          923 g          876 t
ORIGIN      
        1 aaatgcagtc tggtggattc tcggcgggat tgccgccttc gcgataatcg gcatcggctt
       61 gctcgtaatg atcatcgcaa ttgcgagtat gagcggtgac accaacaacc gaaacgtttc
      121 caacatcaac tctcgcactg ataacagaaa tatcaacgta accgcgaatg ccaatgtctc
      181 ggagaacgtc aatacaagta gtttgccggc cgagtttagt gacgatttta gccagccgaa
      241 gtggggtgtt ggcgattcaa agtttggacg aatctggtat gccgacgacg agtatcacat
      301 gagttcgaag gagcggactt acatcgttat gtacgctccc tcggacgagt acaacacaga
      361 aaatgcgacg gtcaaaatta cggctcgcag tgtcaacggg tcagtacctt catccggttt
      421 tggcttgatg gttcattgtg tccagtcaaa agcaaaacaa cttgaagact atgctttgct
      481 gctttatccg ggcgagacac ctcaatacga agtaataatg cacaaggatg gaaatcaatc
      541 ctccctggtc tccaaaacca gctcgagtgc aattcgccct gggtcgaatc ccaatcagtt
      601 ggaagttagg attaaaggga cagaaatcgc attctatgtg aatggacagt atttaacccg
      661 gatcactgac acggcaaact atcgccgcgg gcgagtcgga ctttacacca gtgacgtcac
      721 tgacattgca ttcgacgatc tggagatcaa acggtaatca aagtttcatg agatgcaggt
      781 caacctcaaa gtaatcgctg gtccatacat gggccgcagt ttttccttca atcaacacga
      841 cagtttcctc atcggtagaa ctcccgaagc tcatctttgc ctggccagtg atcgatactt
      901 ctctcgtcat cattgcctgc tcgagatcaa tccaccccac tcgtttctgc gcgatctggg
      961 ttcaaccaac ggcacgtttg taaacggtca gcgagtacat gaaacgtcac tgcggcatgg
     1021 cgaccgcgtc cagggtggtg aaactatctt gctggtggaa gttcaggctt caacatctga
     1081 cgatctcgat gaaactacgc gcgacgcatt gccgcccacc cgaccggtga tggtccaggt
     1141 tgaatgtctg aactgtggtc gacgagatta tgctcaagca tcagatgaaa acgaacggtt
     1201 gaatttcatt tgcgaagagt gccggatcga gttgaagaat gcgccacaag gtgtgccggg
     1261 tttcgatacc atcaggatcc tgggccgagg cggaatgggt tgcgttgtgt tggcgcgtca
     1321 acaccaaaca ggaagagacg tcgcaatcaa aactcttttg ccggagtttg cagtcagcga
     1381 taaagcattg cgtcgattca tgcgcgagat ggatgttgcg gcagctctta agcacaaaaa
     1441 cattgtcgag tttattgatc gtggtactca caatggcgtg gtgtacctgg taacggagtt
     1501 cgtggatggg ccagacgcct cccggctggc ggaagtcagc ggcggacgcc tttcttatcc
     1561 ggacgcgatt tcggtcatct cgcaatcgct ggacgcattg gcctacgcgc ataaccaggg
     1621 ctatattcac cgcgacttca aggatcaaaa catcctcgtc gccggaaaat caccgaatct
     1681 tacggctaag ctaactgact ttggactggc taagagtttt actcactctg gaatgagcgg
     1741 agtaaccatg gccggcgaga tggccggaac gttagcctat atgccgcccg aacagcttcg
     1801 caattttaga gacgtcaaac cgcaatccga tatctatgct gttggtatga ctgcttatag
     1861 tctgttaacg ggcacgcttg cactcgacct tgcaaagaag acaagtatca acgacaccat
     1921 tcgcgcgatc ttcgaacaac cagcagtgcc tttgcgagaa cgggcaaacg aaattcccca
     1981 aatggtctgc gatattatcg atcgtgctct cgcaaaggat ccgacccaac gttggcaatc
     2041 cgctacggcg atgcgtaatg cactgctcca cgctctctaa gccattttta cgggcgattg
     2101 aacatcgact cgtcaacagg aacgttgtgt ctcacatcct cgaggctaaa tacgtatgta
     2161 gttttgccaa ttgtttgtgt ggtcttgaag ggaatcttca ctccgtcgac gtcccgccaa
     2221 tcgctgtagt agaactctgt aagtgacata ccctgtgagg ttttccgctg tgtttcacgg
     2281 cgcgtcaaaa gaccggtctc ttgatcaaaa tataagtact cagggagtcc cgctcgtggt
     2341 gtagcttcga ctttgaagag ttcgcgaaaa ccttcgtgca cccggctcac gactttcatc
     2401 gactgataca gctgtttgag ttttagatct ctgtagaagt cagaatcgac cgcaagtgat
     2461 gctagttcga aaccgacgat tggttgtgag cccgctttca ccgaaacgtc ccagccagct
     2521 cgaccgtcga atcctcgttt gaagagtcca atcggttctg cctgcataat catcagagat
     2581 ttgttgggcg cctttgaata agtctccagc tttcctccaa agctcactcc gactacatca
     2641 actctgccac gtgctacccg gctgtttagt ttcgattgag catctcgtcc acctcgcacg
     2701 gtaacgtaac gatcgagaat tgaatcgagc gaaggcaagg aagcgacaac agcaggagca
     2761 actaatattt ttgaaggcgt cgtttgcttt gtcgcattaa ctacagcgcg cgaactagca
     2821 tcacgaacaa ggttcggagt tccaactttc cgggcgggca tagaaacagc gctctgcata
     2881 gcgacggcaa actcggctgt agttaagttg ggagcgccac gtctgaacgg cgatatttgt
     2941 ctgggagctg ctgatgcttt ctgaattacg aagtaagaag gatagccagt cacgccgtag
     3001 cgaactgcga gttccctctc agcccgtcca tgctcgggat tgatgcgtac cttaacaact
     3061 gttcgtaaat agtctcggac tggttgcgat ggcaggtact gattgtcgag ggagcggcag
     3121 taaggacacc agtcagtgta gaaatagaca atgagaggtt cgtttgattc ctgctgaagt
     3181 ttgagcgcct gttcgtatcc gggtgcgtcc cgcaaccaac ggtcaggatc ggtgtcgcgg
     3241 ccggcgaacg gcaaagtggt aatcagaatg agtaaaagag agattgagag tttcattcga
     3301 aaaggcctta ttccgggttc aagtgagtga ggcaggtcgg gcaacaacta agatacctat
     3361 aaggccctcc ggctatcaag acttttccgc aacagacgtg ggtatccttt tcgaacgccg
     3421 aataccccta acggcagtta ggggatcctt aaagtcccac ctaacgagaa tcgtgcaatt
     3481 attgggggtt gcgctttgta ggttggactt tatggatccc ccaactcccg ttgggggaat
     3541 tctaagagct atcgcggact tcttctttgt aggttggact ttaacgatcc cccaactccc
//
LOCUS       DASSFP010000172         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_39474, whole genome shotgun sequence.
ACCESSION   DASSFP010000172 DASSFP010000000
VERSION     DASSFP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39474"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFH91_03720"
     CDS             complement(<1..355)
                     /locus_tag="VFH91_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6862122.1"
                     /translation="MKYLLLIYQDEQSWSGLTEQERRDIYAEYGKFTEEIKASGHYVG
                     GSQLHPASTATSVRVRNGKALTTDGPFSETKEQLGGYYLVEAKNLDEAIGLASRIPSA
                     RAGTIEVRPLVEGTAS"
     gene            complement(443..958)
                     /locus_tag="VFH91_03725"
     CDS             complement(443..958)
                     /locus_tag="VFH91_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6862123.1"
                     /translation="MAIDDKNQPLLSETSCVQYVRVASADDAAEITRVINAAFRQAEG
                     FFVDDDRITLGGVGGLLDTGTFIVIECDQQLIACVYLESRDDRAYLGLLSVDPGFQRL
                     GLGSVLMNASEDHCRREGLNSIDINVVNLREELPAFYRKRGYVETGTSPFPMDVKTKI
                     PCYFIEMSKPL"
     gene            1272..1865
                     /locus_tag="VFH91_03730"
     CDS             1272..1865
                     /locus_tag="VFH91_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6862124.1"
                     /translation="MRSKCCLILGLLLLSAASLNAQVVDPMTGKWLGESRSLFVLRSD
                     SFVTDKDSIRITKLLDQTPAADPVTVTLQKNFSQVSTWIAKAADMVPADKYSFKATPD
                     VRTFGQLIAHIADSANFFCARAAGNKVEYSDAIEKGTTDKATLVPKLKASLDTCNATY
                     KNPTKVEELITNLGHVNLHYGNIITYMRLMGMVPPSS"
     gene            complement(2014..2655)
                     /locus_tag="VFH91_03735"
     CDS             complement(2014..2655)
                     /locus_tag="VFH91_03735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6862125.1"
                     /translation="MGLILYFLRHGQTELSRDDTFCGSGLDPELTPEGLQMAQAFANA
                     YRTTEWRAIYSSSLRRAITTAQPLCEAIGATLNQSPRLNEIGYGKWEGMTKAKVEQQY
                     HDDYITWLADPAWHAPTDGELAVVIARRGMQLIEEIKEQYDSGNVLVVSHKATIRIIL
                     CALLGIDVGRFRYRLVCPVGSVSIVEFTKEGPLLRAIADRSHFSEELKTLPGT"
     gene            2961..>3455
                     /locus_tag="VFH91_03740"
     CDS             2961..>3455
                     /locus_tag="VFH91_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6862126.1"
                     /translation="MTSIRLYCPQLLGLVLFLTVLSFAQTQVANAATVPTGFTDSVVA
                     SGLSSPTAMAFAPDGRIFVCQQGGALRVIKNGALLATPFLTVTVNSSGERGLLGVAFD
                     PNFASNQLVYVYYTATTPTIHNRLSTFVASGDVALAGSETVILELNDLSGATNHNGGA
                     IHFGP"
BASE COUNT          842 a          918 c          820 g          875 t
ORIGIN      
        1 cagatgccgt tccttcaacc aacggacgca cctcaatcgt tccggcgcga gcggaaggaa
       61 ttctggatgc gagaccaatg gcctcatcga gattctttgc ctcgactaga tagtagccgc
      121 ccaactgctc cttcgtctca gaaaagggac catccgtagt tagggcttta ccatttcgaa
      181 ctcttacact agtcgcggtc gatgccggat gaagctgtga acctccgacg taatggccac
      241 tcgccttgat ctcttcagta aacttgccat attccgcgta aatgtctcgc ctttcctgtt
      301 ccgtcagacc ggaccatgat tgctcgtcct ggtaaatcaa taataggtac ttcatagttg
      361 cctcccttaa aaatttgact ttcacttgta cgacgggcga gatcctgaaa atcgacaatc
      421 gcgatgctaa ttttttggca gctcaaagtg gttttgacat ctcgatgaag taacacggta
      481 tcttcgtctt tacgtccatt ggaaatgggg atgtcccagt ctcaacgtaa cctcgcttac
      541 ggtaaaaagc cggcagctct tcgcgcaggt ttaccacgtt aatgtcgatg gaattgagcc
      601 cttccctacg acaatggtcc tccgaagcat tcattagcac tgagccaagt ccaagtcgct
      661 gaaacccggg atcaaccgac agcaatccga gataagcgcg gtcgtctcgc gactcgaggt
      721 agacgcaggc aatgagctgc tgatcacact caataacaat aaaggttccc gtgtcgagaa
      781 ggcctccgac accgcccagg gtgatgcggt catcatccac aaagaaccct tcggcctgac
      841 gaaacgctgc gttaatcacg cgggtaatct cagccgcatc gtccgcgctc gccactcgaa
      901 catactgaac acacgaagtc tcgctcaata aaggttggtt tttgtcgtcg attgccatag
      961 cgcctgggag atcagaatac ctactctcgg cctgataaca caaagcactg agcgtgttta
     1021 agcccagggc aattgttaaa agttgtgtcg tcaaaaccct ggagcaaccg ctctaatcga
     1081 ttcgttcgga tttaatgacc tttcgcatca ctgaaaggac cattcacggc aattccattg
     1141 cctgaacccg gtgtagataa gcatattgca gtggcacgac cgcgcgatat ttatggcttc
     1201 tacgatcggt tttccgaggt cggttccaag actctaatcc agcagatttc aaaagcagag
     1261 gagatatgga aatgagatca aagtgttgtt tgatactggg attgctattg ttgtcagcgg
     1321 cttcccttaa cgcacaggtt gtcgatccaa tgacgggaaa atggcttggt gagagtagat
     1381 ctttatttgt gttgaggtct gattcattcg ttaccgacaa agactctatc agaatcacaa
     1441 aactattgga ccagacgccg gcggctgacc cggtcacggt tactcttcag aagaatttca
     1501 gtcaagtgag tacctggatc gctaaagcgg ccgatatggt tcccgctgac aagtacagtt
     1561 tcaaggcgac tccagacgta cgcacttttg gccaactcat cgcgcatatt gccgattcgg
     1621 cgaacttctt ttgcgcgcga gccgctggaa acaaagttga gtattcggac gctatcgaga
     1681 agggtactac tgacaaagca acgctagttc ccaaactcaa ggcatctctc gacacctgca
     1741 atgcgaccta caaaaatccg acgaaagttg aagagctaat tacaaacctt ggtcatgtga
     1801 atttgcatta cggcaacatc attacctaca tgcgcctgat gggtatggtc ccaccgtcaa
     1861 gttaagtttg gagtgtggcc ggcgccgctt tgttatatcg gctctcgggc tttcgaattt
     1921 gaatttcggt tttggagtgc gacaaccctg gcgttagcct tgttactcga acgcgccggt
     1981 ataacaaagc gacgccgggc ctcgcactcc aaattaggtt ccgggaagag ttttgagctc
     2041 ttcgctgaaa tgtgaacgat cagcaattgc gcgtagcaga ggtccctcct tagtaaactc
     2101 aactatactc actgaaccta cgggacaaac aagtcgataa cgaaagcggc cgacatcgat
     2161 gcccagcagc gcacacaaga tgatcctgat tgttgctttg tgcgaaacga cgaggacgtt
     2221 cccgctgtcg tactgttcct ttatctcctc aatcagctgc atacctcgcc gagctatgac
     2281 cactgccagt tcgccatcgg tgggagcgtg ccacgccggg tctgcgagcc aggtaatata
     2341 atcgtcgtga tactgctgct ccactttggc tttcgtcata ccctcccatt ttccgtagcc
     2401 aatctcattc aaccgcgggc tttgatttaa cgttgcaccg atagcttcac acagcggttg
     2461 agcagtagta attgctcgac gcaaactgct cgaatagatg gctcgccatt cagttgtgcg
     2521 atatgcattg gcaaacgcct gggccatctg tagaccttca ggtgttaact ctggatccag
     2581 acccgagccg cagaacgtgt cgtctcgact aagctcggtc tgaccatgac gtaagaaata
     2641 gagaattagt cccatcacaa actccgcgta atatatttaa cgcctcaact atcgcccgct
     2701 gttgtagcgg tccgcaaatc tcacgcgcct atttatgatg ctcagcagta tgtaattcag
     2761 atcgagttta cacgcacctg tacagggttc actaaaacga attggtagcg acacttactt
     2821 ttgaaccttg tacgcaaaac tttcgtcaca ttcggatggc aagtttcaag ccttccgcaa
     2881 gcagacaccc cgtcattcaa tcgcgacgcg gaatcgctaa cctcgaccac ttttaaccga
     2941 attcttctgg agggttctct atgacgtcaa ttaggttgta ctgtcctcaa ttgctgggcc
     3001 tcgtgttgtt tttgacggtc ctctcgtttg cgcaaactca agtcgctaac gccgcaaccg
     3061 tgccaacagg cttcactgat tcagtggtgg ccagcggctt aagtagtcct actgctatgg
     3121 catttgcacc cgacggtcgc atctttgttt gccagcaagg cggcgcgctg cgagtgataa
     3181 agaatggcgc gctgttagct actccgtttc tcaccgttac cgttaactcg tctggagagc
     3241 gaggactgct cggagtggct ttcgatccca atttcgcctc gaatcagttg gtctatgtct
     3301 attacaccgc tacaacaccg acgatccaca accgccttag cacgtttgtc gcctcgggcg
     3361 atgtagcgtt ggctggtagt gagacggtga ttcttgaatt gaacgatcta agtggcgcga
     3421 caaatcataa tggcggcgcc attcactttg gacca
//
LOCUS       DASSFP010000173         9008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_782007, whole genome shotgun sequence.
ACCESSION   DASSFP010000173 DASSFP010000000
VERSION     DASSFP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9008
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782007"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFH91_03745"
     CDS             <1..249
                     /locus_tag="VFH91_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862127.1"
                     /translation="AIAAGTSQYGMQTFDQSLFHLLQSRHITLEEALHNASNPDEFKM
                     RVAGIFSGDQSITNLTTPQTMNSPSPPPPREESIFVKH"
     gene            complement(255..1790)
                     /locus_tag="VFH91_03750"
     CDS             complement(255..1790)
                     /locus_tag="VFH91_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862128.1"
                     /translation="MFQRLTTQSACEQALIKIRLLLHEHVEWFFSAEQERTQLLRRGE
                     IDLSISRQRLVLSCWTERGIRSWRILEWDWTGERLLLKASRRMGAERPLIELIPRASA
                     SAVAATIRAARQIRCDQLAQLATNLLPHTRIERCALSPGIRRGQPGRYARILLRRKME
                     RIGVTGCVVPSSAAAVDAFLSAAMLWFKRTSDRIKPPYVQQLWLLVPPEMVRAAIYRV
                     ALLRQSLQDLIRVFKVDAGLTSLELVCKLERSQLWKKRLARFPPVPPAKISDQIASIV
                     SLAPEAIDVVHARYGETLRYFGLPFARVRTLLGTEAIWFGLAANSRRRLEQSSITEWE
                     NMIRDLHEHRSAEAVDRRNALYLNAAEAWLESLLRKDITRLDPGLIIAPLHAQFRTAR
                     GGRLGVRPIDLLALRLDGRLVVIELKVSEDREHVLQGADYWRRVEAHRRRGHIAAAKL
                     FGDLPIIDEPPLVYLVAPTLRFHPSFTTLARLIAHDIEIYRFDINENWRSGVQVMRRL
                     RVN"
     gene            1990..3171
                     /locus_tag="VFH91_03755"
     CDS             1990..3171
                     /locus_tag="VFH91_03755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET6862129.1"
                     /translation="MGNIYLAGSVRTPIGKFGGALASLSAADLGVAVAQETLRRTGIE
                     AGQIDQGIWGCARQAGGGPNVGRQITFRAGVPDTVPAFTVNHACGSGLKAIILAAQEI
                     LLGRANIILAGGTESMSRVPYFAEGARWGMRMGNSPLVDGMYRDGFNDPLSGLVMGET
                     AENLATQHSISRSEQDEYALRSQQRAQTAIESGRFSNEIVSLEVNGPKGKNTTFAKDE
                     HPRAGSAIQDLSKLPPVFSKDGTVTAGNSSGITDGAAALLVMDEESLKQNGVKAQARL
                     VDYEIAGVAPEIMGIGPVPAIRRLLQRQKLALKDVDLIELNEAFAAQVIACDRELSFD
                     SERLNVNGGAIALGHPIGCTGVRITTTLLHELEKRDTRLGIATLCISGGMGIALLLER
                     V"
     gene            3240..3899
                     /locus_tag="VFH91_03760"
     CDS             3240..3899
                     /locus_tag="VFH91_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862130.1"
                     /translation="MDRPSTDTPPVVEVVSNTPPFPTREPERYRAVRTIIQTEPGGKT
                     TTLKTNIFRDGPSRREELETANAPTLVYLERSGQRFLLLPDEKIYSEVDSQFALPNSF
                     DDNATESSPDRVLHTEPVQTAYQKLSDEVLDGKTVTRYKVIVNNASSGSVSNSVTLVW
                     IDEKLGMPVKSEMSAANGVQTIMRLSEISLEVNPDDFEIPTSYRKVAVQEIVGHTKRS
                     E"
     gene            3915..5045
                     /locus_tag="VFH91_03765"
     CDS             3915..5045
                     /locus_tag="VFH91_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862131.1"
                     /translation="MEANQSQPEGHPNTTPSKIGLNPYFSHNQRSLEQKGFILVTTKF
                     LALAVIVLTIAGNQLTGHAQPRTRGTAASPSVGLLSDLPESDAVARIKVKRLLNEVMP
                     QMLANSPARLAEANAAIERFKTRTGLDPRMFDELALGVRYTYPTEGITKLKTVALASG
                     SFNAAGMIAAGRIAANGKYREEKYNGKTIYIFTLDENIKVLGLFDARIGELAAVPLDS
                     TTLALGDPTTVRDAIDVNRGRKRANAEINALASHDPDAVIGFGSNVTAQLIQNLNIGN
                     APIANDLSNLRQVYGSVGATARDVELFIGAKAVNADAAKSLGDTLEGLKQLGGFFVGR
                     LSGAKGVLAKSALANLKIVTQANELQIRTAVAQADVGPLMGN"
     gene            complement(5094..7109)
                     /locus_tag="VFH91_03770"
     CDS             complement(5094..7109)
                     /locus_tag="VFH91_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6862132.1"
                     /translation="MLKQLAKLERTRKFVIFGFAALMAISLIIFYAPGRSASNLDPAK
                     NTEVVAKVGSAKVTVADLAQTRENYRQMFGGRISLAQLGGNKRFLEGLISKHVIAQEA
                     ARLGLGASDAELAAKIRKEFSDASGQFIGFDRYKESITARYGDIEKFENEMRDQIAQE
                     KLRAFVSASVNVSDDEVQEEYKKRNTTFNVSYVVVSADKLAEKIQPSDDELRAYYESH
                     KTDYRYLEPQKKIRYVFINTEKAGSKLQISDADLKTEYDKLQPQFKEAGVKVQQILLK
                     VARKDLDPQVEQKAKDIIAKLRGPDGKASEQAFAEAARGQSEDPATAKNGGFLAQPVK
                     RNPNIKAEGLYERTLDMLPGDVSDIPIRFKGNWYILRRGDSVPKTFEEAKPELLVSLR
                     NRRGFQTAFELATKAEARLKETKDPQKVAQEFAAQANMSPAEMVRETPYIKPQDDVPG
                     IGSNQQFEQAIEALNNANDVGNATGVKDGFAIPMLVEKKDPRIPEFDEVKSKIVETIK
                     KQKATEQVEQKAKDLVASVSSADGLKAAGEKEGLTADAQESYKLGSSLGSAGSSTALD
                     DAIYGLKSGEVSKTPVKVDNNWVVVGVTKRTEADLAEFAKQRDSLRQSMLSERQGQVF
                     EDYVAGVQERMKREGKIKIYEDVLATLEEDEEPAPPMIPGGLNFPTK"
     gene            7325..8416
                     /locus_tag="VFH91_03775"
     CDS             7325..8416
                     /locus_tag="VFH91_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET6862133.1"
                     /translation="MDAISGSTWLWVGFSVFIVCMLSLDLGLFNRRAHAIRYREAWIW
                     SAVWVTLALIFAGLVLHYQGSQRGLEFLTGYLIELSLSVDNLFVFLLIFSYFKVPSKF
                     QHRVLFWGVLGALFMRLTMIFVGAALLNRFHWIIYIFGGFLVYTGIKMFKQEEMDIHP
                     DQNPIVRFVTRLFPISRSYDESKFFTTVNGKRTGTLLLLVLIVVEVTDLVFAVDSIPA
                     IFAITTNTFIVYTSNVFAILGLRSMYFLLAGVVEKFHYLRTGLAIVLTFIGCKMLLVA
                     AGVHIPILFSLGFVALVLLGSVVASLIFAKEQDTTFHVDLPPDFDLPLDEDSLDAEGD
                     SLASEESDAENESNVSEGTTSEKNEKETV"
BASE COUNT         2201 a         2310 c         2204 g         2293 t
ORIGIN      
        1 gctattgccg ccggaacatc gcaatacgga atgcagacct tcgatcagtc gctgttccat
       61 ttactccaat cgcgacacat cactctcgaa gaggctctgc acaacgcgtc gaatcccgat
      121 gagttcaaga tgcgagttgc gggtattttc tcaggcgacc aatcgattac aaacttgacg
      181 acgcctcaaa ctatgaatag tccgtcacca ccaccgccca gggaagaatc gatcttcgtt
      241 aagcactaag caatctagtt aactcggagt ctcctcatta cctgtacgcc cgagcgccaa
      301 ttttcattaa tatcaaagcg gtagatctca atatcgtggg cgatgagccg tgcgagcgtt
      361 gtaaacgacg ggtgaaatct caaagtggga gccactaagt aaacaagtgg tggctcatca
      421 atgatgggca aatccccaaa cagtttcgcc gctgcaatgt gccctctgcg tcgatgagcc
      481 tcgactcgtc gccagtaatc tgcaccctgc aagacatgct cgcgatcctc cgaaactttt
      541 aactcgatca caaccaatcg accatcgagt ctcagtgcca acaagtcgat tggacgaact
      601 ccaagcctgc ccccacgagc tgttctaaac tgagcgtgca acggcgcaat gatcaaccct
      661 ggatcaagcc gggtgatgtc ttttcgcagc aatgattcga gccatgcttc agctgcattg
      721 agatataacg cgttgcgacg gtccacggct tcagcagatc gatgctcatg taagtctcgg
      781 atcatgtttt cccattctgt aatcgagctt tgttcaagtc tgcgtcgact gttggctgcg
      841 agcccaaacc agatcgcctc ggttccaagc aaagttctga ctctggcgaa aggcagtccg
      901 aagtaacgca gcgtctcgcc atagcgagca tggaccacat cgatagcttc aggcgcgagc
      961 gacacgatcg acgcgatctg atcagagatt ttagcgggag gcaccggtgg aaatcgcgca
     1021 agtctcttct tccacaattg cgatctttcg agcttacaaa ccaactcgag cgaggtgaga
     1081 ccggcatcta ccttgaaaac tcgaatcaga tcctgaagac tctggcgtaa taacgcgacg
     1141 cggtagattg cggcccgaac catttcaggc ggcacaagaa gccagagttg ttgtacgtac
     1201 ggaggcttga ttcgatccga tgtgcgtttg aaccagagca tcgctgccga caagaaggca
     1261 tcgacagcgg cggcgcttga ggggacgacg caaccggtaa ctcctattct ttccattttt
     1321 cggcgcagga gaattcgcgc gtagcgacct ggttgtccgc gtcttatacc tggacttaat
     1381 gcgcaacgct caattcgggt atgtgggagc agattagttg ctagctgggc caattgatcg
     1441 caacgtatct gtcgcgccgc tcgaatagtt gcggccaccg cgcttgctga cgcccttggg
     1501 atcaactcta tcaacggacg ttcggcaccc atacgtcgcg atgccttcaa caataaccgc
     1561 tcgccggtcc agtcccattc gagtatgcgc caggaacgaa tacctcgttc ggtccagcat
     1621 gagagtacta atcgctgccg cgagattgag agatcaattt ccccgcgcct gagcagttga
     1681 gtgcgttctt gttcggctga gaaaaaccac tcaacgtgct cgtgcaacag caggcgaatt
     1741 ttaataagag cctgttcaca tgcagattga gttgtaagtc gttgaaacat gacgtgagga
     1801 acatgttttg gtgaacttcg tggttacttc taccgtgtgc tacatttttt ggcaaggaaa
     1861 ttgtgggtgg tcagtggtca gtgatcagtt tgtttcattg ttttgagtgg ggaccagaca
     1921 caagcgatta agggactacg gacacggact acggacacgg actacggacg gcgaactacg
     1981 gaaaaaagta tgggaaacat ctatttagca ggctcggtaa ggacaccaat cggaaaattt
     2041 ggcggcgcac tcgcctcatt gtccgctgcc gaccttggcg tcgctgttgc tcaggaaact
     2101 ctgcgccgca ccggtatcga ggccggccag atcgatcaag gaatatgggg ctgcgcacgc
     2161 caggccggtg gtggtccgaa cgttggaaga caaattactt ttcgcgccgg cgttcccgac
     2221 actgttcccg cgttcacagt taatcatgcg tgcggatctg gtcttaaggc gatcattctc
     2281 gcagcccagg aaatcctgtt aggtcgcgcc aacattattc ttgcgggcgg cactgaaagc
     2341 atgtcgcgcg ttccctattt cgcggaagga gcccgttggg gcatgagaat gggtaactct
     2401 cctctggttg atgggatgta ccgcgacggt ttcaatgatc cactatcagg actggtgatg
     2461 ggcgagactg ctgagaacct tgcgacgcaa cactcgatct cgcggtcaga acaagatgag
     2521 tacgcactac ggtcccaaca gcgcgctcaa acggccatag agtcggggcg ttttagcaac
     2581 gagatagttt cgctcgaggt aaatggtcca aaaggaaaaa atacgacatt cgcaaaagac
     2641 gaacacccga gagcaggctc cgccattcaa gatctctcaa agcttccgcc ggttttttcg
     2701 aaggatggca cagtaaccgc cggcaattct tccggtatta cggacggagc agccgcactt
     2761 ctggtcatgg atgaagaatc gctcaaacaa aacggagtga aagctcaggc gcgtctggtt
     2821 gattatgaaa ttgccggagt tgctcctgag atcatgggta taggtccggt ccccgctatc
     2881 cgacgcttgc tgcaacgaca aaagcttgcg ctgaaagacg tcgatctcat cgaactcaac
     2941 gaagcattcg cagcccaggt gatcgcgtgc gatcgtgaac taagctttga cagcgaacga
     3001 ttgaatgtta acggaggggc catagcgctg ggccatccga tcggatgcac cggcgtccgc
     3061 ataactacca cgttgcttca tgaacttgaa aagagggata cgcgactcgg aattgcgaca
     3121 ttgtgtatta gcggtgggat ggggatcgcg ttgctactgg agcgagtata gtgctgaact
     3181 gcaagcgctc ttcagtgctc ttattgcttc ttatctccag tattacgtcc tgtcgtcaag
     3241 tggatcgtcc ttcaaccgac accccgccag ttgtagaagt tgtatcaaac actcctccgt
     3301 ttcctacccg ggaaccagaa cgttaccggg ccgttcgaac cattattcag actgagcccg
     3361 gcggtaaaac gactactctc aaaacgaaca ttttcaggga tggtccatca cggcgtgagg
     3421 agttagaaac cgccaatgcg cctacactgg tgtatctcga aaggtctggt cagcgatttc
     3481 tattgctacc cgatgaaaag atttattcgg aggttgattc gcaatttgca ctaccaaact
     3541 cattcgacga taacgcaacc gaaagttctc cggatcgtgt actccacact gagccggtcc
     3601 agaccgcata tcaaaagttg tcagatgagg tgttggacgg aaaaaccgtc acgaggtaca
     3661 aagttattgt taacaatgcg agttccggat ccgtcagcaa tagtgtgacc ctggtttgga
     3721 ttgacgaaaa attggggatg cctgtgaaat ctgagatgtc ggcagctaac ggagttcaaa
     3781 cgataatgcg gctctcggaa atatctctgg aagttaatcc agacgacttt gaaataccaa
     3841 cttcctatcg gaaagtcgct gtgcaggaaa tagttggcca tacaaagcgc agcgagtgac
     3901 ggcttctcgt taaaatggaa gccaaccaga gtcaacccga agggcatccg aacacaactc
     3961 cgagtaaaat aggcttgaat ccctatttct cgcataacca gcgatcactt gaacagaaag
     4021 gctttattct ggtgacaacg aagtttctcg ctctagccgt tatcgttttg acaatcgccg
     4081 gcaaccagct gacgggtcac gctcaacctc gcacgcgagg aaccgcggcc agcccatcag
     4141 tagggctact ttcagatttg ccggaatctg atgcggtggc tcgcataaag gtaaagcggt
     4201 tgctcaatga agtgatgccg cagatgttgg cgaatagtcc cgctcgcctg gcagaagcca
     4261 atgcggcaat tgaaagattt aaaactcgta ctggcctcga tccccgaatg tttgatgagt
     4321 tagcactcgg cgttcggtat acatatccga ctgaaggtat tacgaaatta aaaaccgtgg
     4381 cgctcgcgag tggcagcttt aacgctgccg gtatgattgc agccgggcga attgccgcca
     4441 acgggaaata tcgggaggag aagtataacg gcaagactat ttacattttt acgctcgacg
     4501 aaaacatcaa ggtccttggt ctattcgacg cccgaatcgg agaactagca gcggtaccac
     4561 tcgactcgac cactctcgca cttggtgatc caacaaccgt tcgcgatgca atcgacgtca
     4621 atcgcggtcg caagcgcgca aatgccgaaa ttaatgcttt ggccagtcac gatcccgacg
     4681 ccgtcatcgg tttcggcagc aacgtaactg cgcagctgat ccaaaacctg aacattggta
     4741 atgctccgat tgcaaacgat ctctcaaatc tcagacaagt gtatggatct gttggcgcaa
     4801 ccgctagaga tgtcgagctc tttattgggg caaaggcggt aaatgctgac gccgcaaaaa
     4861 gtcttggcga cacacttgaa ggcctgaagc agttaggtgg tttttttgtt ggaaggctat
     4921 cgggagcgaa aggcgtcctt gccaagagcg ccctggcaaa cttgaaaatc gtgacgcaag
     4981 ctaacgaact tcagatccga actgcagtgg cccaggcaga cgttggacca ctgatgggaa
     5041 attgaaagag ggaataggtc ctataagtca tataagacct attcttcaaa actctacttc
     5101 gttgggaagt ttagaccacc gggaatcatc ggaggtgctg gctcttcgtc ctcttccaga
     5161 gtggcaagta catcctcata aatcttaatc ttaccttcgc gcttcatacg ttcctgcact
     5221 ccggcaacat aatcttcgaa aacctgaccc tgccgctcac taagcatcga ttgccttaat
     5281 gaatcgcgct gttttgcaaa ttctgccagg tcggcctcgg ttctcttggt tacaccgaca
     5341 acaacccagt tgttatcaac cttgaccggc gtcttgctaa cttctccact cttcagaccg
     5401 tagatcgcat cgtcaagagc agtgctggaa ccggccgaac ccaaagacga acccaacttg
     5461 tatgattcct gagcatcagc ggtgagtcct tctttttcac ccgcggcttt caacccatca
     5521 gcactgctga cggaggcaac cagatcctta gccttctgct cgacctgctc agtcgctttc
     5581 tgctttttaa ttgtttcgac gatcttgctc tttacttcat cgaattcggg gatgcgtgga
     5641 tcctttttct caaccagcat tgggatggca aaaccatcct taacacccgt ggcgttaccg
     5701 acatcattgg cgttgttcag cgcttcaatg gcttgttcga actgctggtt actaccaatc
     5761 cccggcacgt catcctgagg tttgatgtag ggagtttcac gcaccatctc tgcgggcgac
     5821 atgttcgcct gggccgcgaa ctcctgggcc accttctgcg gatctttcgt ttcctttaaa
     5881 cgagcctcag ctttagtggc gagctcaaag gccgtctgga agccacgacg gtttcgcaaa
     5941 gataccaaca attccggctt ggcctcttca aacgttttcg gaaccgaatc gccgcgtcgc
     6001 aagatatacc agtttccttt aaatcgaatc gggatatcgg acacatcacc gggaagcata
     6061 tcgagggtcc gctcatacaa cccctcagct ttgatgttag gattacgttt cacgggttga
     6121 gccaaaaacc caccattctt cgccgtcgcc ggatcttctg actgacctcg tgctgcttcg
     6181 gcaaacgctt gctcagaggc ctttccatct ggtccacgaa gttttgcgat gatgtccttt
     6241 gccttttgtt caacctgtgg atccaggtct ttacgcgcaa ctttcaacag aatctgttga
     6301 actttaactc cagcttcctt gaattgcggc tgaagtttgt cgtactcggt cttaaggtcg
     6361 gcgtccgaaa tctgaagctt tgaccctgct ttttcagtat tgataaagac gtaacgaatc
     6421 ttcttctgag gctctaaata acgataatcc gtcttgtgac tttcataata cgcgcgcagt
     6481 tcatcgtccg aaggttgaat cttttctgcc agcttatcag ccgatacgac tacgtaactt
     6541 acattaaaag tagtgttgcg ctttttatat tcctcctgga cctcatcatc agaaacattg
     6601 acgctggcac tcacaaaggc tcgcagtttt tcctgagcaa tctgatctct catttcattc
     6661 tcgaacttct caatgtctcc gtagcgagcg gtaatcgact ctttatacct gtcaaaacca
     6721 ataaactgac ccgacgcgtc cgaaaattct tttcgaattt tcgcggcgag ttctgcatca
     6781 ctcgcaccca gaccaagtcg tgcggcttct tgagcgatca cgtgtttact tatcagacct
     6841 tcgagaaagc gtttgttgcc accaagctgc gcaagactta tgcggccgcc aaacatctgg
     6901 cggtagtttt ctctagtctg agccaggtcc gctacagtga ctttcgccga gccgacttta
     6961 gcgaccacct cggtattttt agcgggatcc aaattactgg ctgaacgtcc cggagcgtag
     7021 aagatgatca agctgatagc catgagggcg gcaaagccga agatgacaaa cttgcgggta
     7081 cgttcgagct tagctagttg tttaagcatt tttcttacct ttcaagagga acggcggatt
     7141 gtaagggaga cgcctaaacg cctgcaactc cagacaatga tcgttcgaca accgagatgt
     7201 ggcagttgtt gatttgatcg agctttcacc ttcaatactt aaccaattat taattatcaa
     7261 ttcacaatta ataattagag ggtatttgat cggattgaaa ctgaatgcta ttttaaaact
     7321 ctgaatggac gcaatcagcg gctcaacgtg gctctgggta ggcttcagtg tattcattgt
     7381 ttgcatgctc agcctcgatc ttggcctttt taatcgacgc gcccacgcta ttagataccg
     7441 ggaagcctgg atctggagtg cggtttgggt aacattggcc ctgatctttg cgggcctcgt
     7501 tctgcattac cagggttctc aacgtggact ggagttcctt accggttatc taatcgagtt
     7561 atcgctctcc gtcgataatc tttttgtctt tctcctcata ttctcttact tcaaggttcc
     7621 ttcgaagttc caacaccggg tgcttttctg gggcgttctc ggcgccttgt tcatgcgctt
     7681 aacgatgatc tttgtcggcg ccgccttact caaccgattt cattggatca tctacatctt
     7741 tggcgggttc ctggtgtata ccggaattaa gatgttcaaa caggaagaga tggacatcca
     7801 cccggatcaa aatccgattg tccgtttcgt gacgcggttg tttccaatta gtcgctcgta
     7861 tgacgaaagt aaatttttca ctacagtcaa tggcaaacga accggaactc tattgttact
     7921 ggtattgatc gttgtcgaag ttactgacct cgtatttgcc gtagactcaa tcccggccat
     7981 ctttgcgatc acaaccaaca cctttatcgt ttacacctca aacgttttcg ccattctcgg
     8041 tttgaggtct atgtatttct tgttggctgg cgtggttgag aagttccact atctgcgaac
     8101 gggccttgcc atcgttctaa cgtttatagg gtgcaagatg ttgctggttg cggccggcgt
     8161 acacattcca atcttattct cgttgggctt cgtcgccctg gtgttgctgg gttctgtcgt
     8221 tgcctctcta attttcgcga aagagcagga cacgaccttt catgtcgatc tgccgccaga
     8281 tttcgaccta ccgttggacg aagactcatt agatgctgag ggtgactctc tcgcaagcga
     8341 ggaatcggat gctgagaatg agtctaacgt gtcggaggga acaacttcag aaaagaacga
     8401 gaaggaaact gtttagactc ttcacgcggc gggtcgaact cttccaaaga caccatttga
     8461 acctcacaaa attcatttac attagtccaa tatcgaaccg ccgcaaagaa acctcaaaac
     8521 caaaataacg agagcgttat aatcggtgcg accctctcct gataccgtgg cgtcatgcgg
     8581 aattctaaga cgggcgctgg atattgttaa atagttctca aaaacggttg aattgtcctc
     8641 gttcgtagtg atcagcgata aaagtattaa acgtgcttga acgacactgt ttttgtatgg
     8701 taaattcttg agtcggtagt tgttaatgcc gaacccttgg caagcacaac ccacctaccg
     8761 gtcaccgttt tagaatttca ctccttttaa tgtgcgaaac aagcctttca atttgtcgct
     8821 gagacaccaa attagaacgt ctcgaaagct ttcgcccctc tgacgtaagt cgttgcaata
     8881 atggccacta gaaccagtcg attcgcattt gattggtgga taattcagaa gagatttctc
     8941 tatctggttg ccgccatcat gctgctttgc ggagttgccg gcggcgctgc tctgtatgtt
     9001 tggaagta
//
LOCUS       DASSFP010000174         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_39748, whole genome shotgun sequence.
ACCESSION   DASSFP010000174 DASSFP010000000
VERSION     DASSFP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39748"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..1392)
                     /gene="corA"
                     /locus_tag="VFH91_03780"
     CDS             complement(382..1392)
                     /gene="corA"
                     /locus_tag="VFH91_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET6862134.1"
                     /translation="MEILVYRPGTTQVQEEFTKEQLPELLKDKSAVIWVDMEAPTADD
                     ERILLDVFKFHPLTVEDCRENRHYPKVEEFPDYVYFIVHGVRADTSPEAFNTIELDGF
                     LGPNYVITYHHDFFRSIKNVKQLLRTSPVACQRGAPFLLHQILDQVVDFYSPVLDSFD
                     EKIDLLEENIFALKTPNNQILAEIMDLKRGVLRLRRISTKQMDILHRMSRGEFHLIPA
                     DMLPFYRDIYDHLVRVTDLAESYRDLIGGSLDAYLSVVSNRLNEIMKVLTIFSAVMLP
                     LTFIAGVYGMNFENMPELHSRYGYYAVWGLMVLVAVAMLSFFWRRGWIGRGAKEDQAG
                     EG"
     gene            1522..3117
                     /gene="serA"
                     /locus_tag="VFH91_03785"
     CDS             1522..3117
                     /gene="serA"
                     /locus_tag="VFH91_03785"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET6862135.1"
                     /translation="MTTKIFVADDVSESGLEPLRAASFTVEKRTGLKGEELARALQDC
                     EGLIVRSETKVGPDLLEAAPSLRVIGRAGVGVDNIDVASATIRGVVVMNAPDGNTITT
                     AEHTIALLISLARSIPQASSSLKSGKWERKKFIGVELQGKTLGIVGLGRIGRVVASRA
                     RAMGMFIVAYDPFIAPEQARDLEIELAPLEEVYKRADFLTVHTPLTAETRGLINRQVL
                     SKMKQGARVINCARGGLVDEEALYEAIKSGHIAGAAIDVFTQEPPSPDNPLLELDQVI
                     VTPHLGASTTEAQEGVAVTVAEQMRDFLLTGALRGAVNVPVVGLKELKLMQPYIELAD
                     RLGRFQAQLVDQAVGEVKIEFAGEIVDIDAAPVTRAFLAGLLRDVSARVNVVNAFLIA
                     DERGIKVTTTYVRTSGDKTPAIHTEIFTEHSSQSLAGTLFGSGGDRREGRITEIDGFH
                     LEATPQGHMLVMRNRDVPGVIGRVGTILGQDGVNISRFHLGRQERGGEAIAVIEVDVP
                     VTAKTLDALRSSESVLSARPIEL"
BASE COUNT          881 a          833 c          837 g          798 t
ORIGIN      
        1 tttttgttag gcgctttggg tgcgttgagc gggtttctgc ttagctcgct ggtgaattac
       61 aactatggag atgccgaggt tgcaatgttg ttttggtggt taatgggcgc tgctatggtt
      121 gtggcaggag aaaaaagaga agaagcgtag ggactccttt tcaatttctc atttttcatt
      181 tcgcattttt catttctcat ttctcattat ttttcatttc tcatttttca ttacattttg
      241 ggccacttgg aaccaatagc aattctcaaa tatcaaatca taattctcaa atatcctggc
      301 ctccgaaaaa tgaaaaatga caaatggaaa atgaccaatg acaaataaag accaagctgc
      361 cgcacccaat cccccaatac cttatccttc cccagcctga tcttccttag ccccgcgacc
      421 gatccatccg cggcgccaga agaaagacag catcgccact gccaccagaa ccatcagtcc
      481 ccaaacagca tagtatccgt aacgtgaatg caactcgggc atgttttcga agttcatccc
      541 gtaaaccccc gcgataaacg tcaacggaag catcaccgcc gaaaagatcg tcagcacctt
      601 catgatttcg ttaagtcgat tggaaacaac cgacaaatac gcatccaaag aaccgccaat
      661 caaatcccgg taactctctg ccagatccgt tacacgaacc aggtgatcat agatatcgcg
      721 ataaaacgga agcatgtcag cgggaatcag atgaaactcg ccgcgactca tccgatgcaa
      781 aatgtccatc tgttttgtcg agattcgcct cagacgcaac acgccccgct tcagatccat
      841 aatctcggca aggatctgat tgtttggtgt cttcaaggca aagatgtttt cttcgagcaa
      901 gtcaattttc tcatcaaaac tatccaacac cggcgagtaa aagtcgacta cctggtccag
      961 gatctgatgc aacaggaaag gtgcgccacg ttggcacgct accggactcg tgcgcaacaa
     1021 ttgtttgacg tttttaatac tccggaaaaa gtcgtggtga taagtaatta cgtagtttgg
     1081 gccaagaaaa ccatcgagct cgatggtatt gaacgcttcc ggactcgtat ccgctcttac
     1141 accgtgaaca atgaagtaaa catagtcagg aaactcctca accttgggat agtggcggtt
     1201 ctcacgacag tcctcgaccg tcagcggatg aaatttaaag acatccagta ggattcgctc
     1261 gtcatctgcg gtaggtgctt ccatatcaac ccagataaca gcagacttgt ctttgagcaa
     1321 ttccggtaac tgttcttttg taaactcttc ctgaacctgt gtcgttcccg gccggtaaac
     1381 gagaatctcc atatatggct cctaaggtca actttgtcgg aacgtcaact cgacgcaaat
     1441 tcggcagact gttaccatac cgagctttta ctggcaagac tctttcaaat caagaaaagg
     1501 ttccgaagga ctcaagtata aatgacgacc aagatatttg tggctgatga tgtcagcgag
     1561 agcggtcttg aaccactccg ggcggcatct tttacagttg aaaaaagaac cgggttaaaa
     1621 ggcgaagaac tcgcccgcgc cctccaggac tgcgaaggtc tgattgtaag aagcgaaacc
     1681 aaagttggac cggacttact cgaagcggcg ccaagtctgc gcgtgatcgg gcgcgcaggt
     1741 gttggcgtgg acaatattga cgttgcttcc gcaaccattc gcggagtggt cgtgatgaac
     1801 gcgccagacg gcaacacgat cacgacggcg gagcatacca ttgcgttatt gatttcgctc
     1861 gcgagatcga ttccccaggc ttcgagcagt ttgaagtccg gaaaatggga gagaaagaag
     1921 tttattggcg tcgaactcca gggaaagacc ctcggcatcg ttggtctcgg aaggattgga
     1981 cgcgtcgttg catctcgcgc tcgagcaatg ggaatgttca tcgtagccta cgatccattc
     2041 atagctcctg aacaggctcg tgacctcgag atcgaattgg cgccgctgga agaggtatat
     2101 aagcgcgccg actttctgac ggtacacaca ccactgacag cagaaacgcg tggtctaatc
     2161 aatcgccagg tgctctcaaa aatgaaacaa ggagcgcggg taatcaactg cgcccgcggt
     2221 ggacttgttg atgaagaagc cttgtacgag gcgataaaga gtggtcatat cgcaggagcg
     2281 gcgattgacg tcttcactca ggaaccaccg tcgcctgaca atcctttgct ggagttggac
     2341 caggttatcg tgactccaca cttaggagct tctactactg aagctcagga gggagtagcg
     2401 gtaacagtcg ctgagcaaat gcgcgacttt ctgcttacag gcgcattgcg aggcgccgtg
     2461 aatgtgccgg tggtgggttt gaaggaactc aaactcatgc agccttacat cgagttggcc
     2521 gaccgtttgg gaagattcca ggcacaattg gtggaccagg cagtgggaga ggtcaagatc
     2581 gagtttgccg gcgagatcgt cgatatcgat gcggcgccgg ttacaagagc ttttctcgcc
     2641 gggttgttgc gtgacgtaag tgcgcgagta aatgtagtga acgcattcct gatcgctgac
     2701 gaacgaggta ttaaggtcac gacgacttat gtgcggacca gcggagataa aacgcctgcc
     2761 attcacactg agatctttac cgaacactcg agccagagtc tcgccggaac tttatttggt
     2821 tctggaggcg atcggcgcga gggtcgaatc accgagattg atggttttca tctcgaggca
     2881 acgccacagg gtcacatgct cgtgatgagg aatcgtgatg tgccgggtgt gataggtcga
     2941 gtcggaacga tcctgggtca ggatggcgta aacatcagcc gttttcatct cggccgacag
     3001 gaacgcggcg gagaagccat cgcagtgata gaggtcgatg ttcccgtgac tgcgaagact
     3061 ctcgatgcgt tacgatcgtc ggaaagtgtt ctttcggccc gaccaatcga gctctgaatc
     3121 ggaatcattg gagaattgtc atctgatatt tgagaatttc tatttcgatc caacgagcac
     3181 cccgtaaaca attaacaatt ctcaaatatc aaatgataat tctccaataa ctcctaaccc
     3241 caaccccaac tccaacccct gacccttgat ccccaatgat ttacagctcg attggtcggg
     3301 ccgaaaggac actttccgac gatcgcagcg catcgagagt cttcgcagt
//
LOCUS       DASSFP010000175         5453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1680903, whole genome shotgun sequence.
ACCESSION   DASSFP010000175 DASSFP010000000
VERSION     DASSFP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680903"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..1683
                     /locus_tag="VFH91_03790"
     CDS             472..1683
                     /locus_tag="VFH91_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET6862136.1"
                     /translation="MPTERLYYSDSHLIEFEAKVIDVTERVSGWAAVVLDRTAFYPTG
                     GGQPSDTGTLNLQRVFECIDDGEQGILHVVQGTTPARGTLVKGRVDWARRLDHIQQHT
                     GQHILSQAFVKLFNAPTKSFRVLDQSSEIDLELTNPTTETIERAVELANNVIWEDRTI
                     TIRNVTSDEATDLPLRKEPARTGDLRLIEIEGFDLSPCGGTHAFRTGEVGMIAVRSWE
                     RAKGLTRIEFVAGTRALADYRRANASARAVAALFSTSRDDAPQLAASMVEDNKELQKR
                     VRVLEDIAAGVEAENLLSESQPEGTRIVARVFDKRDAESLKKLAHALIGHPNTIALLG
                     SLDRETARLVFARSADAGGDMSELMRQACEMIDGRGGGKPDMAQGGGKKLDKLVEALA
                     AAQNRLKQLDE"
     gene            complement(1695..2690)
                     /locus_tag="VFH91_03795"
     CDS             complement(1695..2690)
                     /locus_tag="VFH91_03795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6862137.1"
                     /translation="MDQGTELTEFLYRHSLAEPHEQIIWTKLAGGVSSDIWRVDLPGR
                     TICVKRALAQLKVAADWRAPITRNKSEWDWIQFVNRHFPGLVPQPIAHDAELQMLAMQ
                     YLPPSNYPVWKNLLLQGVVDREFATITGQTLAKIHAESTNTPETRELFRTDETFHSLR
                     LEPYLLATAERHPALRETITATADRTARTHIALVHGDVSPKNILVGARGPIFLDAECA
                     WFGDPAFDLAFCLNHFLLKCLARPDLTQDLIIGFAAFFESYLREVTWEERDQLESRCA
                     RLLPMLFLARVDGKSPVEYIDQDSQKDLVRETAYFFINQPSDNLLTIAMHWQGVT"
     gene            complement(2701..3990)
                     /gene="eno"
                     /locus_tag="VFH91_03800"
     CDS             complement(2701..3990)
                     /gene="eno"
                     /locus_tag="VFH91_03800"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET6862138.1"
                     /translation="MTSTVIGSVRGLQVLDSRGRPTVEAEVELASGIRARASVPSGAS
                     TGSAEAHELRDHIPERYEGRGVLNAVANIGTSLAEVLRGRDALDQRQIDELMVQLDGT
                     PNLQRLGANAVLAVSLATCRAAAEAQQIPLYRWIAELVGNKDVSLPLPMVNILSGGLH
                     ARRGMDVQDFLLLPRADSIDEAVHLAVRVRAAADEVAKEYDVTTLLADEGGLSPGFEN
                     GRQALGMMLASFERAGLRPGHDAFIALDLAASSLIDEHGQYHFSREGRTYSSAELINL
                     VEVWAAEFPIISVEDPLGEEDWSAWRTLTTRLGRKLQIVGDDLFATNLSRLRRGISEG
                     AANSVLIKLNQNGTLTGTLDVIREARSNRYATIVSARSGETEDSFMVDLAVGTSAGQI
                     KIGSMRGSERLSKYNQLLRIEDQGVPFGGSHQVPAPQ"
     gene            complement(4031..5353)
                     /locus_tag="VFH91_03805"
     CDS             complement(4031..5353)
                     /locus_tag="VFH91_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862139.1"
                     /translation="MYRKMAIRFFALLACLLSVGYAAAQNCEIKTRPAVVDKSFESIF
                     SQDGPGSGLQSTSGPAWTGADSTYSIVLPNGDSAFFFSDGYIAESPPVEGDGKVIVAA
                     NGMRMRQANCFPPVCTPATSLFRSRNSIVVLSKRLDRMKTFTGPPDKEGYSTSLFTPE
                     KAAETKHYFWMGDSVVVKTENPSVDKLWVFLLEFDPALAYYGTSIAQLSLPDLKIESI
                     QKLRSTQQSTVAWGSALLLERSNDGPALYIYGIQNKQSINGKVPYLARVNPDTGVNSV
                     ADASNWKVWSGSDWVSGLNNAAQLIGDKKDPKNSGDQISDEFSVKKIRTAKGSVYVLV
                     GMDTRLPFGKWKDITLYSACRPQGPFSAKQVVYSTPETDSRKLPGMAPDQQLAGPLVV
                     YNPHLHPQFNNRDGLLISYDTNITKNEDLLYADSYRPRFIRVPIEGLR"
BASE COUNT         1355 a         1429 c         1368 g         1301 t
ORIGIN      
        1 taattcgact tcgatggata cctcaaatag atgtgaagtc ggtctggaca ttgaaaggtt
       61 gtacttgatc gaacgcaatg ccgaattctg gccaaaaacg gcttgcgcac agaagagagc
      121 cactagaact actagtggac catagaccag ccggggtgaa gaaactctca ttcacaaatc
      181 tccatcaaca aggttgcgaa gcgggaagat ggtctaatta tgtttagtga aacagaaact
      241 gtcaaaatta atgcaattct atccaccgat ttcgccgatg acgcagcttt gaacttttag
      301 ttgggatgtt actcggcttg gtcatctcac ttgtcaaagc ggcgtaatcg tcgaaatctg
      361 ggagaggata ttactcggct tgctcacctc aatcgtctag tctgcgaaat cggcgaaaac
      421 tgtggatgac tcgacttgca tatcatggcc tcgctcgttt attactgaga catgccaacc
      481 gaacgtcttt actattcgga ctcccacctg atcgagtttg aggcaaaggt gatcgacgtg
      541 acggaacgag ttagcggttg ggccgccgtt gttctcgatc gcaccgcgtt ttatcccacc
      601 ggcggcggtc aacccagcga tactggaacc ctaaacttgc agcgtgtttt cgaatgtatc
      661 gatgacggcg aacaaggcat cctgcatgtt gttcagggca cgacgccagc gagaggcact
      721 ctggtaaaag ggcgtgtcga ctgggcccgt cgcttagacc acatccaaca acacaccggg
      781 caacatattt tgtcgcaagc tttcgtaaaa ctctttaacg ctccaacgaa gagttttcgt
      841 gttctggacc agtcatccga aattgatctg gagcttacca acccaactac cgagacaatc
      901 gaacgcgcgg tcgaactggc taataacgtc atctgggaag atcgcaccat cacgattcgc
      961 aatgttactt ctgacgaggc gaccgactta ccgttacgta aagaaccagc gcgcaccgga
     1021 gacctccgct taattgagat cgaaggattt gatctaagtc cgtgcggcgg aacacatgcc
     1081 tttcgcaccg gagaggttgg aatgatagcc gttcggtcct gggaacgcgc aaaagggttg
     1141 accaggattg aatttgtggc aggtacgcga gcacttgcag actatcgcag agcaaacgcc
     1201 agcgcacggg ccgtcgccgc tttgtttagt acaagtcgag acgacgctcc tcaacttgcc
     1261 gcgtcgatgg ttgaagacaa taaagagtta cagaaacgtg tacgagtgct tgaagacatt
     1321 gctgccggag tggaagccga aaacttatta agcgagagtc aaccagaggg tacgcgaata
     1381 gtagcccgag tctttgataa acgtgatgcc gagtcgttga agaaactggc gcacgcgttg
     1441 attggtcatc caaatacaat tgctttgctc ggctcgttgg atcgcgagac tgccaggctc
     1501 gtctttgcga gatcagcaga tgccggcggc gatatgagcg aattgatgcg tcaagcctgc
     1561 gagatgattg acggccgcgg cggtgggaaa ccagatatgg cccagggcgg cggcaaaaag
     1621 ctcgataaat tggttgaggc tcttgctgcc gcgcaaaata gacttaaaca gttggatgaa
     1681 taagtgccga gcattcacgt aacaccttgc cagtgcatcg caattgttag gagattgtct
     1741 gagggttgat tgataaagaa gtacgcggtc tctcgaacaa gatctttctg actatcctgg
     1801 tcgatatatt ccactggcga ttttccatcc accctcgcca gaaacaacat tggcaacaat
     1861 cgagcgcacc tcgactccag ttgatcgcgc tcttcccagg tcacctcacg caggtacgat
     1921 tcaaaaaatg ctgcgaaacc gataataagg tcctgagtaa gatcgggcct tgcaagacat
     1981 ttcaggagaa agtgattcaa acaaaatgct aagtcgaatg ccggatcacc aaaccaggca
     2041 cactctgcgt cgagaaaaat tggaccacgt gcgccgacca gaatgttttt tgggctcacg
     2101 tcaccatgaa ccagcgcaat atgagttcgt gcagtccgat cagcagttgc tgttattgtc
     2161 tctcgtaacg cgggatgtcg ctcggcagtc gcaaggagat aaggttcaag tcgaagcgaa
     2221 tgaaacgtct cgtctgtccg aaacaattct ctggtttcag gagtatttgt gctctccgca
     2281 tggatcttgg cgagtgtctg tccagtgatg gtcgcaaact cgcggtccac tacaccctga
     2341 agtaatagat tcttccaaac cggatagttt gatggcggca gatattgcat cgccaacatt
     2401 tgcaattcag catcgtgggc aatcggctgg gggaccaatc caggaaagtg ccggtttacg
     2461 aactgaatcc aatcccactc gctcttgttt cttgtgatgg gcgctcgcca atcagctgcc
     2521 actttcaact gtgccagcgc gcgttttacg cagatcgttc tgcctggaag atcgactcgc
     2581 caaatgtccg acgagacacc gcccgcaagc ttggtccaga taatctgttc gtgcggctcc
     2641 gccagtgagt gacggtaaag aaactcggtt agttcagtgc cttggtccat cggggacaat
     2701 tcattgcggg gcaggcacct ggtggctgcc gccgaagggt acaccctggt cttcaatgcg
     2761 cagtagttga ttgtatttgc tcagacgttc tgagcctcgc atcgaaccga ttttgatttg
     2821 accggcagaa gttccgacgg caaggtccac catgaaactg tcttccgtct cgccggatct
     2881 ggctgaaact atggttgcat agcgattact ccgcgcttca cggatcacat ccagagttcc
     2941 cgtcaaggtt ccattttgat ttagcttaat cagaacgctg ttggcagcgc cttcgctaat
     3001 accccttcgc agacgactta aatttgtcgc gaataggtca tcaccaacaa tttgaagttt
     3061 acgtcctaat cgagttgtca aagttcgcca cgccgaccag tcttcctcac ctagaggatc
     3121 ttcaactgag atgatgggaa attccgcggc ccacacctct accaggttta tcaactcagc
     3181 tgacgagtat gttcgaccct cgcgtgaaaa atgatattgc ccatgttcgt caataagact
     3241 tgaagcggcc agatcaaggg caatgaatgc gtcgtggccc ggtcgcagcc ccgctcgctc
     3301 aaacgacgcc aacatcatac ccagtgcctg tcttccgttc tcaaatccag gacttaaacc
     3361 accctcatca gcaagtaacg ttgtcacgtc gtactctttt gcgacttcat cagctgctgc
     3421 acgaacgcga acggctaaat gaactgcttc atcgatcgag tccgcacgag ggagtaagag
     3481 aaaatcctgc acatccatcc cacgccgggc gtgcaagcct ccactcaata tgttgaccat
     3541 cggcagcggc agcgagacgt ctttgtttcc aaccagttca gcaatccagc ggtacaacgg
     3601 aatctgctgg gcttcagctg ccgctcgaca cgtagccagg gaaaccgcca acacagcatt
     3661 cgcacctaag cgttgcaggt tcggagtacc atcaagctgc accatcaatt cgtcgatctg
     3721 ccgttggtcc agcgcatctc ttcctcgcag gacttcagcg aggctcgtcc cgatgttcgc
     3781 aaccgcattt aaaacacccc gcccttcata tctctcggga atatgatcgc ggagttcgtg
     3841 agcttcagca gatccagtgg atgcccctga cggaacagaa gcacgcgccc gtattccact
     3901 cgcaagctct acttcagctt cgactgtggg tcttccccga gaatcgagaa cctgtaagcc
     3961 ccgaactgat ccgatgaccg tgctcgtcaa atttatacct acatcaaata ctcggctgcg
     4021 gtttgctcaa ttatcgcaga ccttcaatcg ggactcgaat aaacctgggc cgataactgt
     4081 ccgcgtaaag caagtcctca ttcttagtga tattcgtgtc gtacgaaatc aatagaccat
     4141 cacgattgtt gaactgcggg tgcaagtgtg gattgtagac caccagtggg ccagctaact
     4201 gctgatctgg ggccatgcct ggcagcttgc gagaatcagt ttcaggagtg gagtaaacga
     4261 cctgttttgc cgaaaatgga ccctgcggac gacacgccga atagagtgtg atgtccttcc
     4321 atttgccaaa tggaagcctc gtgtccatcc ccaccagaac gtaaacgctt cctttagctg
     4381 ttcgaatctt ttttacgctg aactcgtcgc ttatttgatc gccagagttt ttagggtctt
     4441 ttttgtcccc gataagttgc gccgcattgt tcaggcccga aacccagtca ctgccactcc
     4501 agactttcca gttcgatgca tcggcgacac tatttacgcc cgtgtctgga ttgacgcgag
     4561 caagataagg aaccttgcca ttgatactct gcttattttg aattccatag atgtaaaggg
     4621 ctgggccatc attggatctc tcgagcaaca atgccgatcc ccacgcaaca gttgactgtt
     4681 gcgtgctgcg taacttttgg atcgattcga ttttcagatc aggcagcgat aactgcgcta
     4741 ttgaagtgcc gtaataggca agagctgggt cgaactcgag caggaaaacc catagtttat
     4801 caacgctcgg gttttcggtt ttgaccacaa ccgagtcacc catccaaaag tagtgttttg
     4861 tctcggcggc tttctccgga gtaaatagcg aggtcgaata accctccttg tcgggtggac
     4921 ccgtgaaagt cttcatgcga tccagtcgtt tgctcagaac aacgatgctg tttcgcgatc
     4981 ggaacagcga tgttgcagga gtacagactg gtggaaaaca gttggcctga cgcattcgca
     5041 taccattcgc ggcaacgatg accttaccgt cgccttccac tggtggtgac tcagcgatat
     5101 atccatctga aaaaaagaaa gcggaatctc cgttgggcag gactattgaa taggtactat
     5161 cagcgccggt ccaggctgga ccactggttg actgtaaacc gctgcccggt ccatcttgag
     5221 agaagatcga ctcaaaactt ttgtcgacaa cggcgggccg agtcttgatc tcgcaattct
     5281 gggcagccgc ataccccacg ctcagcaaac acgccagcag cgcaaaaaac cgaatggcca
     5341 ttttcctata cattattttt aaaaggtgag ttcaggtgtg cggttgtcgt tcggatccgc
     5401 ccaacgactg ttggggacga taggagtcca atccggtcgt agcggatcac gat
//
LOCUS       DASSFP010000176         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1233530, whole genome shotgun sequence.
ACCESSION   DASSFP010000176 DASSFP010000000
VERSION     DASSFP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233530"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFH91_03810"
     CDS             <1..227
                     /locus_tag="VFH91_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862140.1"
                     /translation="VESSILDTFVQQQSEFISRMNATANLDLDKIVITSPVASVVTYS
                     LIDAYRIIVVHEELHFQQAQRVTSESGFPR"
     gene            357..740
                     /locus_tag="VFH91_03815"
     CDS             357..740
                     /locus_tag="VFH91_03815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6862141.1"
                     /translation="MQIEQINTPNAPQPAGHYSQGIKYNGLVFVAGQLSIDPKTGNKR
                     LGPIEEQTEQALNNVHAILRAAGSDWNRVLKVTVYVSDINDWEAVNRVYSRILGDNRP
                     ARAVIPTGALHYGFLIEIDAVAAAD"
     gene            1056..2876
                     /locus_tag="VFH91_03820"
     CDS             1056..2876
                     /locus_tag="VFH91_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6862142.1"
                     /translation="MSILHKSPNKTIEATVREHQQPADGGKLIVLTAPLTEAIDHAGY
                     FIQMAMASLPIWMEGVLNRKYPKWREVEYNDDGSARYMPAGVRLVEKSLLREYSEDDI
                     VACFPADLDKFIGPRTRVVAVSTHNPLGVTFAAGVYTSIFGSSRMPINSHYSREMFAQ
                     LKASPYRENFKVIVGGSGGWQIVQTDTYEELSVDCVVEGRSESVDTLKLFRSAIDGED
                     LPRRIEVKHPLDREEILFPDTRTTFGVVEMTTGCGRRCKFCVPDLNPQIDLPKDKIMD
                     AVRANVRNGNKQISLATEDMFIWGQVHTDTPFYFPNREALVDLYSDIVNTPGVEQHVL
                     SHCTIAPAVVDPLLIKQLSDLLLPKSPIHFPLLSSHPEKKALAPLIGLETGSVRMAKQ
                     IMSSKGVPFSIEDWPSVVLEGLRVLNENNWFPAMTLIVGNPGETDEDVMETLDLIYEI
                     EKRGLFAFMIPSIFTPLHDTRMANSEGVTQTRQLTPLQWQLMMKCWKMNLRPGQYSWW
                     GPMAWRVGSIGMWLYKLRQLNGPHFTWPLLMFSGAFSEKNLQRMGKIYRGKPFKAKTR
                     AELLASLKPKDWQFLRADNGDMPAAYTTSIPVRAMAAEHV"
     gene            2956..>3216
                     /locus_tag="VFH91_03825"
     CDS             2956..>3216
                     /locus_tag="VFH91_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862143.1"
                     /translation="MHTYLLRLLVAAVTFTVGLAVSAIPFLHTSKVRDFNYERRHDCS
                     KRYFEPSVGVRQDASEPLRVTYVGPGGSIQNGDVPTARLLVQN"
BASE COUNT          827 a          739 c          843 g          807 t
ORIGIN      
        1 cggttgaatc ctcgatactg gataccttcg ttcagcagca atcagaattc atctcaagga
       61 tgaatgcgac tgctaatctc gatttagaca agattgtcat aacttcacca gttgccagtg
      121 tggtcacgta cagcttgatc gatgcgtatc gaataattgt tgttcacgaa gaactgcatt
      181 ttcaacaggc tcaacgagtg acaagtgaga gtggtttccc aaggtaggga ggaacggagt
      241 agcctcgtat ttggctttgc acaaaacgcg aaaacccact cacggacagt gggcggattc
      301 gcggcggtga cccaggtgcg attgacggag ggtcaagctc gcactatcgt ctgaccatgc
      361 aaatcgaaca gatcaacacc ccaaacgctc cgcagccagc aggccattat tcacagggaa
      421 taaagtacaa cggacttgtg tttgttgcgg gtcaactgtc aattgatcca aaaacgggca
      481 acaaaaggtt gggcccaata gaagagcaaa ctgagcaggc tctaaacaac gtacacgcca
      541 tacttcgggc cgccggaagc gactggaatc gggtcctaaa ggtgactgtc tacgtctcgg
      601 atatcaacga ctgggaggct gtaaatcgcg tttattcacg aatcctcggc gataaccgac
      661 cggctcgagc tgttattcca accggggccc tacattacgg atttctaatc gagatcgatg
      721 ctgttgctgc agctgactaa aacccgagcg gcgatgccct taatccatga gagatgtttc
      781 tggtccgacc ggaaaccggg agtgttctaa gctgcaggcg tcttgtcagg tacgctaaaa
      841 tgggggtgtt caagaaggta cgccgccaca gattaggaca ccacccggaa acttaaggct
      901 tgtaaagaac agttcttttg attagtgtag ctgcccgaag ttattcatag agaagttcat
      961 cttgtttatt caaggcgcaa atttcgaccg tacaatagat ctgaatgcgc ggccttcgtt
     1021 tttattgaag cccttaaacc aattggaaaa actgaatgag catcctccac aaaagcccca
     1081 ataaaacaat tgaagcaact gtacgtgagc atcaacagcc ggctgatggc ggaaagttaa
     1141 tagtgttaac tgcaccgtta actgaagcga tagaccatgc tggctacttc attcagatgg
     1201 ctatggcgtc gttaccgatc tggatggaag gcgttttgaa ccgaaagtat ccgaagtggc
     1261 gcgaggttga atacaacgat gacggctccg cgcgatatat gcctgctgga gttcggctcg
     1321 ttgagaaatc acttctgcga gaatatagcg aagacgatat tgtcgcttgt tttccggccg
     1381 atctggacaa attcattggg ccgagaactc gagttgtagc cgtatcgact cacaatccgc
     1441 tcggcgttac ttttgccgcc ggagtttata catcaatttt tggttcttcg cggatgccga
     1501 tcaactcgca ttactcgcga gagatgttcg cccaactaaa ggcgtctccc taccgagaga
     1561 actttaaagt gattgtcggc ggttcgggtg ggtggcagat cgttcaaacc gatacttacg
     1621 aagagttgag cgtcgattgt gtggttgaag gtcgaagtga gtcggtggac actttgaaat
     1681 tgtttcgcag cgcgattgat ggtgaggacc tacctcgtcg gattgaagtt aagcatccgc
     1741 tcgatcgtga agaaatactt ttccctgata cgcgtacaac gtttggtgtg gtcgagatga
     1801 caacgggttg tgggcgtcgc tgtaaattct gtgtgccgga cttaaatcct caaatcgatc
     1861 tgccaaaaga caagatcatg gatgccgtcc gagcaaatgt gcgtaacggg aacaagcaga
     1921 tctcgcttgc tacggaagac atgttcattt ggggacaggt tcacacggac acaccgttct
     1981 attttcctaa ccgtgaggcc ttggtagatc tgtattcaga tatcgttaat acccctggag
     2041 tcgaacagca cgtattgagt cattgcacga ttgctcctgc ggttgtggat ccactcttga
     2101 tcaagcagct atccgatctg ttgctgccga agagtccaat acatttccca ttactcagta
     2161 gtcatcctga aaagaaggcc ctggccccat tgattgggct cgaaacaggc tcagtacgga
     2221 tggcgaagca gataatgtcg agtaagggtg taccgttttc aattgaggat tggccaagcg
     2281 ttgtgttgga aggattgcgt gtcctaaatg aaaacaactg gtttcctgcg atgactttga
     2341 tcgttggtaa tcccggcgag accgatgaag acgtgatgga aactttggat ttgatctatg
     2401 aaattgaaaa acgagggctg tttgctttca tgattccctc gattttcacg cccttgcatg
     2461 acacgcgaat ggcgaattca gaaggagtta cgcaaactcg acaactaaca ccactgcaat
     2521 ggcagttgat gatgaaatgc tggaaaatga atctccgtcc gggtcagtac agttggtggg
     2581 gtccgatggc ctggcgggta ggttcgattg gcatgtggct ttacaaacta cgccaactta
     2641 acggaccgca ctttacttgg ccccttttga tgttttccgg agctttctct gaaaagaatc
     2701 tccagcgaat ggggaagatt tatcgtggaa agccatttaa agctaagact cgcgccgaat
     2761 tactggcttc gttaaagcca aaggattggc agtttttgcg cgctgataat ggcgatatgc
     2821 ctgcagccta cacaacttcg atccccgtgc gggcgatggc cgcagaacac gtgtagtcgt
     2881 cggacaaccg cgcggcgggc tgttgcttct aaccgaaaaa ccggttgaca acttgcccag
     2941 gaaaggtgtt ttggtatgca tacctacctg cttcgacttt tggtagccgc ggttacattc
     3001 acagttggtt tagcagtgag cgcgattccg ttcctgcata cttctaaagt cagagacttc
     3061 aattacgaac gtagacatga ctgttccaaa cgatactttg agccttcagt gggagttcgg
     3121 caggatgcga gcgaaccact tcgggtaacg tacgtggggc cgggtggtag tattcaaaac
     3181 ggagacgttc ccacggctcg cttactcgtt cagaat
//
LOCUS       DASSFP010000177         7940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1681674, whole genome shotgun sequence.
ACCESSION   DASSFP010000177 DASSFP010000000
VERSION     DASSFP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681674"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..901)
                     /locus_tag="VFH91_03830"
     CDS             complement(110..901)
                     /locus_tag="VFH91_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6862144.1"
                     /translation="MELGLKNRVALVAAASQGIGYACALDLAREGARVFLCSRNAERA
                     SEAARKIHEETGAAVAGIAADITDDAAAEAFVTLAREQAGSIDVLVTNGGGPPAGVFD
                     DVDVRTLREAFELTVVSAIRMAKLVLPTMKQQKHGRIINITSMAVKQPIDGLLLSNTM
                     RAGLTGWSKTLSNEVAQDNITVNNVAPGYTLTEQQHEHAITRARKLGKSKEEMLGVWA
                     AQAPINRLANPEEIAAAVTFLASDRASYITGITLQVDGGWSKGLL"
     gene            complement(914..1474)
                     /locus_tag="VFH91_03835"
     CDS             complement(914..1474)
                     /locus_tag="VFH91_03835"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862145.1"
                     /translation="MRLKLGIMGSAAESGRPDVGTALTEAAANLGRAIASHEIILLTG
                     ATTGIVYLVGKTAHDAGAFHIGISPASNQSEHEAYFKLPVDACDSIIYTGFGLKGRNV
                     VLVRSCDVVIFIAGAMGSLNEFTIAHDEGKIIGCLTGSGGVADEADYLLSKFARRQKA
                     QVFQNSDPEKLIGQCLEALKDISNPL"
     gene            complement(1464..2840)
                     /locus_tag="VFH91_03840"
     CDS             complement(1464..2840)
                     /locus_tag="VFH91_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6862146.1"
                     /translation="MLTASKKKPAAAYKSIGYVELLKNNRSFRQLWLGQVVSQMGDWF
                     DTIALYTIILQLTGSGRDIGLLLVARFLPSFLFGPLSGVLADRFSRRSIMIVSDLLRA
                     VVVLGFLFVKSADQLWIIYVLTVFQLGLSTFFEPAKTAAIPSIVTDRELVTANAISSV
                     TWSVMLTLGAAIGGLITGWFGTSVAFILDALTYLLSAALIASIKVPKRKKRERQKLSL
                     GRLLGVSETLEGVSYVRSRPRVLALLLVKPAWGLGGGILTLLPVFGERIFPVGKSAAT
                     GIGVLFAARGIGTAVGPIVARRIAGEGDNQMESSIGIAFIIGGIFYIAFGNALGFVFA
                     LIVLGIAHCGGSILWVFSTILLQRSVADNFRGRVFAAELALLTLTMAASNYATGEFLD
                     RFLYSPRTVTTAIGIFFLLPGIAWFLTRPWWDTAKPAKRSTKAGIDQNNIPVVHQSIE
                     RELKEDAT"
     gene            3724..5739
                     /locus_tag="VFH91_03845"
     CDS             3724..5739
                     /locus_tag="VFH91_03845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6862147.1"
                     /translation="MMELRPPPDSHKREAFSLIKGFASDWWNANQLLISWTMLFGALI
                     TAMAYGVVYSAFYSYILLTPLVIIAAYLIYRSGVSRLQTKNREVELLSHLHLATAEAL
                     ATAIDAKDQTSHCHVRRVQHYAAGMGEIFGLSSQEIAALKSGALLHDIGKLAVPPHIL
                     NKPGRLTPAEFEKMKIHTVVGAQILSRVDFPYPVIPIVRHHHEQWDGAGYPDQLKGDE
                     IPITARIISVVDCFDSVREDRPFRRGMTMEEAVALLKRGAGVHFDPKVVDEFLKHLPR
                     FESEINELGLNTKEDHNQESEPIELNYVDLPQTRESGAFMAYDQIKKAHREVYSLYEI
                     ARTFGTSLSVEHTLDILVDKIGHIVPFDSCVVYFYEEEKGYATVRHVAGKNAKKLVGR
                     TIAPGEGVSGIALVNRTAVNHLHPSLDFRDINPEAGVKYRSMAALPLFKDEAVIGVVS
                     IYSTELDRYSDDHMRLLETVTLLASDALWNAMQHAQAESNALTDPLTGLPNARYLALR
                     FEEEAARARRSDRLFQVMMLDLDDFKSVNDSFGHKVGDKMLKEVANIVHSQLREYDFL
                     ARCGGDEFVALVQEIAGSQVQELTLRIESAVSKFNLNVGRNRFARVGISIGTATFNTD
                     GETLDQLIAAADREMYRVKSLHRGSQTGPAEAQTAAEARINRMSASG"
     gene            5919..>7940
                     /locus_tag="VFH91_03850"
     CDS             5919..>7940
                     /locus_tag="VFH91_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6862148.1"
                     /translation="MAVPAENPSATLLIVDDDPSSRDRLRDIFEHASYRVVTANEAAS
                     ALRVLKGVRCDLVALDLEMPGVDGLALCKLLRSQPTTGKLPIIALSESDDEARKVQAF
                     SAGADDYITKPSTPGELFSRVGLHVRMSQREWALIGTNRELRFLSDIGRGLLRALEPE
                     QLVRRVAGATYEETDASLCAAYVTVGRDKHAACVFDREGSADDPSLLNRVVLHDWLRS
                     TESANSVLLTDKSRFFLNDEKHEAEFVAPLRFAGRAKGALIVAFDHRENCSDAERRLI
                     DAAAQQTSLAAHICSLYQAAQETSFNLARQVERRTAETETQRRFIEAIIDSLPLSLYA
                     IDRQYRVVAWNRNRELGELGIPRGTALGQNVFNVLTRQPREVLEEEFSRVFESGEIER
                     IEQQTPSPKGVRYWLISKIPMWADTSGKVTHVITVGEEVTDRVEANRAVARAEKLAAV
                     GRLAAGVVHEINNPLATISACAESLESRMNEGAFDGSPALEDLRDYLGLIRSEAFRCK
                     SITMGLLDFSRTRASDHVTVNLGDVIGSAVRLLSHQKRNTAVEFSIDITPDLRLVSGD
                     AGQLQQAVIALATNALDAMTLSGTLSISARNEDARVVVEVGDTGIGIAPENLPKIFEP
                     FFTTKEVGHGTGLGLAVCYGILTEHGGTLDVQSTVGVGTTFTITLPAINL"
BASE COUNT         1958 a         2076 c         1991 g         1915 t
ORIGIN      
        1 gttcccaggc ggtattatct aaagcgtttg cgggaagatc ttcgccagcg tacagagctg
       61 taactgaatt cagacgattt tctgatgaca tgcttgcgaa cgcttagttt taaagcagac
      121 ccttgctcca tccgccatca acctgaagcg ttattccagt gatgtaggaa gctcggtccg
      181 aagccagaaa tgtgactgcg gccgcaatct cttcgggatt tgcgagtcga tttatcggag
      241 cctgcgctgc ccacacgccg agcatttcct ctttcgattt tcccagtttg cgcgctcgcg
      301 tgatcgcatg ttcgtgctgt tgttccgtca atgtataacc gggcgctacg ttgttaacgg
      361 tgatgttgtc ctgggcgact tcattagaaa gagttttgga ccacccagtg aggcctgctc
      421 gcatcgtgtt tgacaataag agtccgtcga tcggttgttt aacggccatt gaggtgatgt
      481 taataattct gccgtgcttt tgctgcttca tggttggcag cacaagtttg gccatacgta
      541 tggccgaaac gactgtcagc tcgaatgctt cacgaagtgt tcggacatcc acgtcatcaa
      601 aaacgcccgc aggtggtcca cctccattgg ttaccaacac gtcaatagaa ccggcttgtt
      661 ctcttgcaag agtcacaaag gcttcggctg cggcatcgtc agttatatca gcggcgatcc
      721 cggcgaccgc cgcgccagtt tcttcgtgaa ttttacgagc tgcctcgctc gctcgttcgg
      781 cattgcgcga gcaaaggaaa acgcgagcgc cctcgcgtgc cagatcgaga gcacatgcat
      841 aaccaatacc ctggctcgct gcagcaacca gcgccacgcg attctttaat ccaagttcca
      901 taaaaactct ctcttaaagt gggttactaa tatctttcaa tgcctcaagg cattgaccaa
      961 tcaatttctc gggatctgaa ttctgaaaaa cttgcgcctt ttgcctcctg gcgaatttac
     1021 tcaagagata gtctgcctca tctgcgactc caccactacc cgtcaagcat ccgataattt
     1081 tcccttcgtc atgggcaatt gtaaattcgt tgagtgagcc catcgcaccg gcaatgaaaa
     1141 tgacgacatc gcaggaacgc acgagcacca catttcgtcc tttcaatcca aatcctgtgt
     1201 aaattatcga gtcgcacgcg tcaaccggca gtttaaaata agcctcatgc tcgctctgat
     1261 tgctcgctgg cgagattccg atatgaaacg cgcccgcgtc gtgcgcagtc ttgccgacca
     1321 ggtagacaat tcctgtagtg gctccggtca gaagaatgat ctcatgtgag gcgattgctc
     1381 gcccgagatt tgccgcggct tcagtcaacg ctgttcctac atctggtcta ccgctttctg
     1441 ccgccgaccc cataatcccg agtttaagtc gcatcctctt tcagttctct ctcaatggat
     1501 tgatgaacaa cgggaatatt attctggtct attccagctt tagtcgaccg ttttgccggc
     1561 tttgcggtgt cccaccacgg tcttgtcaga aaccatgcaa tccctggcag taaaaagaaa
     1621 attccaatcg ccgtcgtcac tgtgcgaggt gaatacagaa atcgatcaag gaactcacct
     1681 gtagcgtagt ttgatgctgc catagtcagc gtcagtaacg ccaattcggc cgcgaaaact
     1741 cgcccgcgaa aattgtctgc gacagatcgt tgcaagagaa ttgtggaaaa aacccagaga
     1801 atactgccgc cacagtgtgc gataccgagt acgattaaag caaaaacaaa tccgagagcg
     1861 ttgccgaaag cgatataaaa gatcccgcca attataaagg ctataccgat cgacgattcc
     1921 atttggttat cgccttcacc agctattcgc cgggctacta ttggaccaac tgcggtccca
     1981 attcccctcg cagcaaataa aactccaata ccagtggcag cgctttttcc aaccggaaag
     2041 attcgttctc caaacaccgg gagcagcgtc agaattccgc ctcccaatcc ccaggccggt
     2101 ttcacaagca atagagccag aactctcggc cggctcctta catacgaaac cccttcaagc
     2161 gtttcactaa cccccagcag ccgcccgagg gataatttct gtcgttctcg cttctttctt
     2221 tttggaactt taatacttgc gataagtgct gcagacagta aataagtcaa cgcgtcgagg
     2281 ataaacgcaa cgctcgtccc gaaccatcca gttatcagtc ctccgatcgc ggctcccaaa
     2341 gttagcatca cggaccaggt aaccgacgat atcgcattcg cagttacaag ctcccgatcg
     2401 gtcacaatcg aaggtattgc agcggtctta gcgggttcaa agaaagtcga tagtcccagt
     2461 tgaaaaacag tcaacacata aataatccag agctgatccg cgcttttaac aaaaagaaaa
     2521 ccgaggacta cgacggcgcg cagcagatca gagacgatca ttatggagcg ccggctgaag
     2581 cggtctgcca ggacgccaga tagcggacca aacaagaaac ttggaaggaa ccgggcgacg
     2641 agaagtaacc ctatgtcacg tcctgaaccg gtcagttgca gaattatcgt gtacaacgca
     2701 atggtatcga accaatcgcc catctgactg accacctgac cgagccaaag ctgtctgaag
     2761 ctgcgattgt tcttgagcag ttcgacatag ccgatggatt tgtaggcagc ggcgggtttt
     2821 ttcttactag cagttaacat taaatttgag acgcaaggtt caactgaaca ctttcaagta
     2881 acaaagtgtc ggtcacagtt tctctcatgg cttgcaatta atcgcaacct cgctgaaacc
     2941 gttcacaaga ttctgttgaa tatcgagcac taaaacctct gtggtgatga tttttcttag
     3001 gactttgctc accaaaggta cagtgcagca ctatcgcaat ggacacggag tgagtatttt
     3061 cgcagtagcg gctgccgtca cacatcacag cgctggattc gttgccgacc cacgtaccca
     3121 aacagcgtca aaactcggat ttcagtcagt ttcttgttcg tgttatttcg cgtgatgcgc
     3181 gtggatcgcc tgtttacagt ccgagaaaag cgatccacgc gcatcccacg aaataacact
     3241 aacgaagtcc caaagcaaaa ttagattctt catacagctc accgggagtg gtaaggactg
     3301 cgtgagtaag ccaacaccgg gccttaaatc gtattgaccg aaaattcaat ctgacctgtc
     3361 aaaatgctca cgttctgaaa attggcggga tttatcttgg aaagaataat ggttctacaa
     3421 agcgttctgg gctcaacacg tctccgcgga cttcggcacg caagttgaaa aggggaagag
     3481 tgcggctgtg aagccaaaag cttcgcgggc tcgccgcacg ggtccgaggc cgcacagttc
     3541 tctgataaat aatctcggtc ggcgttttga tcgagctaga tatctcaccc agcacggcag
     3601 tttcgggcag tttccgaaac tgccacttgc ccaactttgc cggagacgaa actgagttgc
     3661 tcccagtttg cagtctccgg catgtctgtg gtgcagataa ccacgtttgt tccactccca
     3721 attatgatgg agttacgtcc accacccgac agtcacaaac gagaagcctt ttcattgatc
     3781 aaagggttcg ctagtgactg gtggaatgca aatcaacttc tgatctcatg gaccatgttg
     3841 ttcggtgctc ttatcaccgc catggcctac ggggttgtct atagtgcttt ctacagttat
     3901 atcctgctca ctcccctcgt tatcattgca gcatatctaa tctatcgaag cggcgtttct
     3961 cgtctccaaa ccaaaaatcg tgaggttgaa cttctcagtc acttgcacct ggccaccgcc
     4021 gaagcgctcg caacggcaat agacgcgaag gatcaaacca gccattgtca cgtccgtcgc
     4081 gtgcaacact atgccgcggg aatgggtgag atatttggcc tatcctccca ggaaattgcc
     4141 gcactgaaat ctggtgctct cttacatgac atcggaaagc ttgctgtccc acctcatatc
     4201 ttgaacaaac cgggacgctt gacgcccgct gagttcgaga aaatgaaaat tcacacggtt
     4261 gtaggagctc aaattcttag ccgagtcgat tttccctatc ctgttattcc catcgtccgt
     4321 catcaccacg agcagtggga cggtgccgga tatcctgatc aattgaaagg tgatgagatc
     4381 ccgattacag ctcgcatcat ctcggttgtt gattgctttg actcggttcg tgaggaccga
     4441 ccatttcgac gcggaatgac aatggaggaa gctgttgcgc tccttaaacg tggggctggc
     4501 gtgcactttg atccaaaagt ggttgatgag tttttaaaac atttgcctcg cttcgaatcc
     4561 gaaatcaacg agcttggctt gaacactaaa gaagatcaca atcaagagtc agaaccaatt
     4621 gaactcaact acgttgatct tccgcaaacc agagagagcg gcgccttcat ggcttatgac
     4681 cagatcaaga aggcccatcg cgaagtctac tcgctttacg aaatagccag gacttttgga
     4741 acttcgctga gtgtcgaaca tacattggac attctggtcg acaagattgg acatatcgtt
     4801 ccatttgaca gctgtgttgt ttatttctat gaggaagaaa aaggttatgc gactgtgcgt
     4861 cacgtcgccg gcaagaatgc gaagaagctt gtcggtcgaa cgatagcccc gggagaaggt
     4921 gtcagtggta tcgccctggt taatcgtact gcggtgaatc accttcaccc aagtttggat
     4981 ttcagagaca tcaatcccga agccggtgtg aaataccgat cgatggccgc gttgccactg
     5041 tttaaagatg aagcggtaat cggtgtcgtt tcaatctatt ccacggagct ggatcgttat
     5101 agcgacgatc atatgcggtt gctcgaaaca gtgacgctgc tggcctcgga cgccctctgg
     5161 aatgccatgc aacacgccca ggccgaatcg aatgcgttga ccgatcccct taccggcttg
     5221 cctaatgcga gatatctcgc gctccgcttt gaagaggaag ccgcacgtgc tcgtcgcagt
     5281 gatcgcttat tccaggtcat gatgttggac cttgatgatt ttaagagcgt caacgatagc
     5341 ttcggacaca aggtcggcga caagatgtta aaagaagtgg cgaatatcgt tcacagtcag
     5401 ttgagagaat acgactttct tgcgcgctgt ggtggcgacg agttcgtcgc actggtccag
     5461 gagattgcgg gctcacaggt tcaagaactc accttaagaa tcgaatccgc tgtatcgaag
     5521 tttaacctca acgttggcag gaatcgtttt gcaagagttg ggataagcat tggtacagcg
     5581 actttcaata ctgacggcga gacgcttgac cagctgatag ccgccgctga tagagagatg
     5641 tacagggtaa aatcgctgca tagaggttct caaactggtc cagctgaagc tcaaacagcc
     5701 gccgaagctc gcattaatcg gatgtcagcg tccggctgat acccggtcag gccctgccaa
     5761 agcgccggcg cgtcggctca ctccaaagta aattcttgac atcctgcttg gtcatcgctt
     5821 tcaatcgaat ttagacacgt ttcccgccgt gtcatttctt taggactttg ctcgccttcg
     5881 gggtcacccg atgaatcgcg acttctacga ctgaaagcat ggcagttccc gccgaaaatc
     5941 caagcgccac cctgctaatt gtcgatgatg atccctccag ccgagaccgg ctccgggaca
     6001 tcttcgaaca cgcctcttat cgggttgtca cggcaaatga agcagcctcg gcgttgcgcg
     6061 ttctgaaggg cgttcgttgc gatctagtgg cactcgatct tgagatgccc ggtgtggatg
     6121 gcctggccct ttgtaaattg ctccgctcgc aacccaccac cgggaaattg cccatcatcg
     6181 ctctctccga aagtgatgac gaagcccgca aagttcaggc gtttagtgcc ggcgctgatg
     6241 attacattac taagccatcg actcctggtg aattgttctc ccgggtcggc cttcatgtgc
     6301 gcatgtcaca acgcgagtgg gcactgattg ggaccaatcg cgaactgaga tttttaagtg
     6361 acatcgggcg agggttgctg cgcgcgcttg aacccgaaca actggtccgc cgtgtggcgg
     6421 gagcgaccta cgaagaaact gatgcgtcct tgtgcgcagc gtacgttacg gtgggacgcg
     6481 acaagcacgc agcctgtgta ttcgatcgcg aaggcagcgc tgacgatcca tctctattga
     6541 accgcgtggt cctgcacgat tggctacgct caaccgagtc tgccaactct gtgttgttga
     6601 ctgataagtc gcgtttcttc ttaaacgatg agaaacatga ggcggaattt gttgccccgc
     6661 tgcgatttgc cggaagagca aaaggcgcgt taatcgttgc gttcgatcat cgagagaatt
     6721 gcagcgacgc tgaaagacgt ctgatcgatg cggctgctca acaaacgtcg ttggcagctc
     6781 atatttgctc gctctaccag gcagctcagg aaacgtcttt taatcttgcc aggcaagttg
     6841 aaaggcgaac cgcagagact gaaacgcaaa gaagattcat cgaggcgatc atcgacagcc
     6901 tgccactctc gttatatgcc atcgatcgac agtacagagt cgtagcctgg aaccgcaatc
     6961 gcgagttggg agaattgggt attccccgcg gaacggccct gggccaaaac gttttcaatg
     7021 tgttaactcg ccagcctcgt gaagtattgg aggaagagtt ttcgagagtg ttcgaaagtg
     7081 gcgagattga acgcattgaa caacaaactc cttcgcctaa aggagtcagg tactggctca
     7141 tcagcaaaat accgatgtgg gccgatacga gtgggaaggt gactcacgtt attactgtag
     7201 gtgaggaggt aacggaccga gttgaggcca accgggccgt cgcacgcgct gagaagctgg
     7261 cagctgtcgg cagacttgca gctggtgtgg tccatgaaat taacaacccg ctggctacaa
     7321 tttcggcctg tgcagagtcg cttgaatcgc gaatgaacga aggcgccttc gacggctcac
     7381 cggctttgga agacctgcgc gattatctgg ggctgattcg aagcgaggct tttagatgta
     7441 aatcgatcac gatgggcctg ctcgacttca gtaggactag agcgtcggac cacgttactg
     7501 taaacctggg ggatgttata ggttcggctg tacgcttatt gtcgcaccag aaacgcaata
     7561 ccgcggtgga gttttcgata gacataaccc ccgatttgcg actcgttagc ggagatgccg
     7621 gacagcttca acaagccgtt atcgcactgg ccacgaacgc cctcgatgca atgacactca
     7681 gcggtacatt gagtatttca gcacgtaacg aagacgctcg ggttgtggtc gaagttggcg
     7741 atacgggcat cgggattgca cccgaaaacc ttcccaagat tttcgaacca ttcttcacca
     7801 cgaaagaggt tggccatgga accggtctcg gcctggcggt atgttatggt atcttaactg
     7861 agcacggggg cacgctcgac gttcagtcga cggttggagt tggtactacg ttcacgatca
     7921 cccttccagc aattaatcta
//
LOCUS       DASSFP010000178         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1376132, whole genome shotgun sequence.
ACCESSION   DASSFP010000178 DASSFP010000000
VERSION     DASSFP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376132"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..378)
                     /locus_tag="VFH91_03855"
     CDS             complement(13..378)
                     /locus_tag="VFH91_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6862149.1"
                     /translation="MSNRILCTEDDLDTREMLLVLLENSGYDVVCANDAQQAYSLITT
                     EQFDLLLVDNWMPGGSGLELVRAVRSAGQSTPILFYSGAAAAADKQEALDAGAQGYLT
                     KPIGIADLVSEVERLIGGK"
     gene            complement(729..1283)
                     /locus_tag="VFH91_03860"
     CDS             complement(729..1283)
                     /locus_tag="VFH91_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6862150.1"
                     /translation="MSANAPQAATTTWNIDPVHSIAEFKVRHMMITNVKGQFPAMRGT
                     ITLDENDVTNSRVESSIQADSINTREADRDTHLKSADFLHVEKFPSLTFASTHITRAG
                     DGELEVEGDLTIRGVTRKVVFDVEGPTPPAKDPWGNIRIGLSATTKINRKDFGLIWNA
                     ALETGGILVGDEVTITLDVEAVKG"
     gene            complement(1410..2624)
                     /locus_tag="VFH91_03865"
     CDS             complement(1410..2624)
                     /locus_tag="VFH91_03865"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6862151.1"
                     /translation="MMACDTRPVAKWLLSGARSASESDQALAETCERLRACGVPLWRT
                     DIYVRTLHPDVVGRRFRWQLDKEVLISAATYDVSETTDSRKSLIERVCAEGKLLRRRL
                     ADANFEKEFPLLLELLEEGATDLLATPLIFTDGDVHAVLWTTRQPGGFTDEQIAGIER
                     IVEPLARVAEVRALRRTATNLLDTYVGKRTGQRILAGQIRRGHSEVINAAIWLSDMRG
                     FTSLSDQLSPHVMIDLLNRYFDCQIPVILSNGGEVLNFMGDGLLAMFPTETAEDSAQI
                     CAQALAAAIETRAKVLSMPKLPAMEHLPPVRFGLALHLGEVLYGNIGGGNRLDFTCIG
                     PAVNLAARLEKVAAELDRTIVTSEIFARHCEPHLSPLGQFTVRGLSTPQTVFGLLDED
                     QTGRLAAESKSN"
     gene            complement(2672..>3123)
                     /locus_tag="VFH91_03870"
     CDS             complement(2672..>3123)
                     /locus_tag="VFH91_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862152.1"
                     /translation="EWAKYFWLAVVPLFLVISVESFVVRPHLFSGAIYNPFWWLGLVI
                     VIGSIITLISSLITGREMGAFLGSSFFIVGLLVTGAAALFPVMLHSTLAPENSLTAYN
                     AAANRTSLLLASIWWPMGLALATVYFVFVSKRYMGKVSVQRDTQGFY"
BASE COUNT          784 a          840 c          753 g          746 t
ORIGIN      
        1 tccgttgaac gcttattttc caccgataag cctttcgact tcgctgacca aatccgcgat
       61 cccaataggt tttgtcagat aaccttgcgc accagcatcc agcgcctctt gcttgtcggc
      121 ggccgcagct gcgccggagt agaacaggat gggagtagat tggcccgctg aacggaccgc
      181 gcgtaccaat tcgaggccgc taccgcccgg catccagtta tctacgagaa gtaggtcaaa
      241 ctgttcagta gtgattagag aatatgcttg ttgggcatcg ttggcgcaaa cgacgtcgta
      301 gccactattc tcaagcagga cgagcagcat ctctctcgtg tcgaggtcat cctcggtgca
      361 aagaatacga ttagacatgg gggtccttat gaaaggcaaa cattagtgtc gaccgttctt
      421 ttgaataggg aggttcaaag tatggattgc gggtgactat tctatcacgg cgaaacgcaa
      481 ttcagatgag tattagtcga cgatcctcga ccatctcatg gtcgaaaaag atcgtgttgg
      541 aagtcctcaa gtggccttgg agtagttccc ttctggcagt attgctcaac gtaataacac
      601 cttgccgagg tcgattgtga caaataatcg atcagcgtct cggcgtctct gcggtaaacg
      661 agtcgggctg aattttagct gcagagacgc ggagaacgca gagtctattg atcacaaata
      721 ttcgagactc aacccttcac cgcctcgacg tccagagtga ttgtcacctc atcaccaacc
      781 aaaatgcctc ctgtctcaag ggcagcattc catataagtc cgaaatcctt gcgatttatt
      841 ttagtggtcg ccgaaagtcc tatccgaatg ttgccccagg gatccttcgc aggtggggtg
      901 ggtccttcga cgtcgaacac aaccttccgt gtaactccac gaatggtcaa atcaccttca
      961 acctcgagtt cgccatcgcc tgcgcgcgtg atgtgagtcg aggcgaacgt taggctcgga
     1021 aatttttcca cgtggaggaa gtctgcgctc tttagatgtg tgtcgcgatc agcttcgcga
     1081 gtgttgatcg aatccgcctg gatcgaggat tcgactcgtg aattagtgac gtcgttttcg
     1141 tccaatgtga tcgtgcctct catagcggga aattgtccct tcacattagt aatcatcata
     1201 tgtcgaactt tgaattcggc tattgagtga accggatcga tgttccacgt tgtagttgcg
     1261 gcttggggtg cattcgcgct catacttctc tccttttgct attctgcaat gttgatgacg
     1321 agttggttaa caaactgtct cttaacaatt gatctgctaa ccgtctgtgc agggcggttg
     1381 acaaacgatt cgcacactca aacttgtgcc taattactct ttgactccgc ggctaaacgc
     1441 cccgtctgat cctcatcgag aagaccgaaa acggtctgtg gcgtgcttag accccgcaca
     1501 gtgaattgtc ccaggggcga aaggtgcggc tcgcaatgcc tggcaaagat ctcggaagtc
     1561 acaatcgttc tatcaagttc cgctgctacc ttttccaacc gggcagccag atttacggct
     1621 ggtccaatgc acgtaaagtc caacctgttc ccgccaccaa tgttgccata gagaacttcg
     1681 cccaaatgca aagctaaacc gaagcgcacc ggtggaagat gttccatagc aggcaacttc
     1741 ggcattgaca gaaccttggc tctggtttca attgcagcag ccagagcttg cgcgcaaatt
     1801 tgtgcactgt cttccgctgt ctcggtaggg aacatcgcca ggagaccatc acccatgaaa
     1861 ttcaaaactt ctccgccatt gctcaagata accggtatct gacaatcaaa atatctattg
     1921 agaaggtcga tcattacgtg cggtgacagt tgatctgaaa gggacgtgaa gccgcgcata
     1981 tcggagagcc atatggccgc gttaataact tcagagtgtc cccgtcggat ctgcccggca
     2041 agaatgcgtt ggcccgttct tttcccaaca taagtgtcaa gcaggttcgt cgccgttctt
     2101 cgcaaagcgc gcacttcggc aactcttgcc agtggttcga caatgcgctc tatgccagcg
     2161 atttgctcat cggtgaatcc tcccggctgt cgagtcgtcc acaaaaccgc atgaacgtca
     2221 ccatctgtaa agatcaaagg tgtagccagg agatccgttg caccttcttc gagaagttcc
     2281 agtagcaaag ggaactcttt ctcaaaatta gcatctgcga gtctcctgcg cagtaatttt
     2341 ccctccgcgc atactctctc aatcaagctc tttcgcgagt ccgtcgtctc cgaaacatcg
     2401 tacgttgcgg ccgatatcag gacctcttta tccagctgcc aacgaaacct tcggccaaca
     2461 acgtcgggat gcaaggtccg cacgtaaatg tctgttcgcc acagcgggac tccacaagca
     2521 cgaagacgtt cgcacgtttc ggcgagtgcc tgatctgact ctgaagccga gcgagcgccg
     2581 ctcagaagcc atttagccac tggccgcgta tcacaagcca tcatgaataa tcgtccctca
     2641 aacgcctgcc aagttgagtt taatgcaaca actaatagaa tccctgggta tcccgttgaa
     2701 cgctcacctt tcccatataa cgttttgaaa cgaatacaaa atagaccgtc gccagcgcta
     2761 accccatggg ccaccaaatg gaagcgagaa gcagagaggt gcggttggcg gctgcgttat
     2821 aagcggtgag tgaattctcc ggagcgaggg tagaatgaag catcactgga aacagcgccg
     2881 cggctcccgt aaccaacaat cctacgatga aaaaacttga gccaaggaat gctcccatct
     2941 cccggccggt tattaggctg gatatcaaag taatgattga gccaatcaca atcaccaatc
     3001 ctaaccacca gaacggattg taaatcgcac cgctgaacaa atgtggtcta accacaaaac
     3061 tctcaactga tatcacaaga aagaggggca caaccgccag ccaaaaatac ttagcccact
     3121 ccg
//
LOCUS       DASSFP010000179         6778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_637921, whole genome shotgun sequence.
ACCESSION   DASSFP010000179 DASSFP010000000
VERSION     DASSFP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637921"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFH91_03875"
     CDS             complement(<1..255)
                     /locus_tag="VFH91_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6862153.1"
                     /translation="MVVKSANKSNKSLVNTQELGRSVKRKREQLSLSLRDVADVTGVS
                     ASTLSRIENGTGKPDADNIARLTSWLDVPMERILAGRNPET"
     gene            complement(408..2117)
                     /locus_tag="VFH91_03880"
     CDS             complement(408..2117)
                     /locus_tag="VFH91_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6862154.1"
                     /translation="MKRISAQLALLFLILSLLVPAFVFAQRNQSQSGSTASGTTGSGI
                     PGRRSTRNAGTAATAVVEHDFDEALKVIQDQYVDGKKLNYNDVFKSSIIGMLRSLDPH
                     SNYYDREEFDELKTDQRSEYFGIGASIQNYFRDDKADTFITATFDGSPAHRAGLKYGD
                     RIVEVNGENMSGKSSAEVRDKIRGPKGSTVRLTLERATDKKIEKVDIVRDVVPQPSIP
                     DSYMLKPGVGYIDMTRGFNYTTTDELLDSLDRLHAKGMTSLVLDLRNNPGGFLDQAIH
                     VAEVFLKNGQLILTQKGRNGYRDNSYESRNNSPDTTPLVILINENTASASEIVAGAMQ
                     DHDRALIVGQTSFGKGLVQSIIPLEYGAGLTLTSAKYFTPSGRLIQRDYSNGGFYDYY
                     KHSGANDKSVVKPNGPEKKTDTGRAVYGGGGISPDETVDARTVNLAQRRLLSPTFSFV
                     RELINGRVAGFESLRQQGGIDFRRELQSDEFKITDALYKSFRAFASNDPAYKVSDAMI
                     DRNRSFVELELRFNLVTAAYGRIMGDRVFITTEDPQVVKAVDVLPKARDLAMSSTSSR
                     AEK"
     gene            2440..2871
                     /locus_tag="VFH91_03885"
     CDS             2440..2871
                     /locus_tag="VFH91_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862155.1"
                     /translation="MSSPYHKEEPGTTESVDDLMIRDDDPEANSEAQNRTASSVRDIA
                     SRIPESLSAIGRDISRTIEKAISAKDDYVVAVKISPEAQDKLEQLVQAGVFRSRAEAA
                     GFLIDEGIRVQSALFERVRQKLGEIERLRAELRGMIADRPV"
     gene            2981..4471
                     /locus_tag="VFH91_03890"
     CDS             2981..4471
                     /locus_tag="VFH91_03890"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET6862156.1"
                     /translation="MAPTIQLPPTPIEEFTLANGLRVVLSQDNVIPVVSIAVYYDVGS
                     RNEKEGRTGFAHLFEHMMFQGSENVPKAAHFQFIFNAGGTMNGTTSTERTNYFETLPA
                     NYLPLAFWLESDRMRSLKVTQENLDNQRNAVQEEKRLRYDNQPYVNAFLRMNELIFTN
                     QANAHSTIGSMEDLDAATIEDIKEFFRIYYAPNNAVLSVVGDFDIAQAHDLIEKYFGN
                     IPPQQTPPPVDVSELSHVAIKNETYHDPLAPAPAFVLGWKIPARRTKDFYALSLAGSL
                     LFDGDSSRLYQKFVKGDESVVSIEGGVDERRGPSAFYIFALPKPGEEVGDIQKKIFAE
                     IKVLAKEPPSAEEMEKLRNSLSNDTVRGRQSTMYRAQRLAEFTLYDSKPSLIDTEVDQ
                     YLSITAQDISDAVSRYVDVENRVVLDIIPAAAEEHEAEHAASPQPPGEPHQPSAPPPQ
                     IPEVPALEPESPRHTDVAQIKTNTPTEQPKDPADVPQQSGSGPMLS"
     gene            4475..6169
                     /locus_tag="VFH91_03895"
     CDS             4475..6169
                     /locus_tag="VFH91_03895"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6862157.1"
                     /translation="MSQQENFRTQPPPPLAPRPIVIPTPHETVLSNGLTVVVVEDKRL
                     PLVSYRLALAVGGAYDPPSLPGLTDLLAGLLPEGTESKTSRQIADAVARMGASLSAGA
                     NSDYTIVAASALAHFSDNVLELMADVILNPSFPENEVDLARQNTKESLRQQRAQPSFL
                     ASEMVARVMFGNHPYSVVAPTPESIDRSTRDEFVSFHRAKFVPNNAVFIVVGDVKFEK
                     ILARLEALFSGWNRGDKLAPNFPAPPVRSSRKAYLIDRPGSAQSNIVIANSGIVRTSP
                     DYFPMLLMHTVLGATASSRLFMNLREEKGYTYGAYSNLDARRSAGTFRATAEVRSVVT
                     GDSLKEFFFELERIRNDKVSEKEIADAKSYLTGVFPVRLETQEGLIDQLVQIKMLSLA
                     KDYLETYRDNVQAVTIDQIQRVAQAYVKPDQAALVVVGDGATVLDQIKPYCADIETYN
                     TAGKLKEVGAATPADITGSWAINVDTPLGQSIPATLTIERNDGILSGRISSEMGNANL
                     GEINLEDNSFQAQTSIEMDGHAVAAEIQARFEGETLVGTLKLRHSPPLSFHGEKTS"
     gene            complement(6314..>6778)
                     /locus_tag="VFH91_03900"
     CDS             complement(6314..>6778)
                     /locus_tag="VFH91_03900"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HET6862158.1"
                     /translation="APSRLELIHIDFVSDRRGWIVGDGGTILNTVDGGLTWTKQTVNT
                     TNTLYHVDFRDDKNGWVVGERGTLLRTTDGGQTWTESATNVSVTLLSVQFLNDDDGWA
                     IGRNGTILRTEDQGRTWLPQQSSTKQNLYSLNFNKKIGWVVGGDGTLLRFER"
BASE COUNT         1689 a         1733 c         1691 g         1665 t
ORIGIN      
        1 tgtctcagga ttgcgaccag caagtatccg ttccattggc acgtcaagcc atgaagtcag
       61 tctggcgatg ttatccgcgt cgggcttacc agtcccgttc tcgattcgtg aaagcgttga
      121 tgcggaaaca ccggtgacat ccgcaacatc tcgcaaactt aaactgagct gttcacgctt
      181 tcgtttcacg gagcgcccca gctcctgcgt gttcaccagt gacttattcg acttgtttgc
      241 agatttcacg accacaaccc taacacactc ctggttttag acgcaagact ttgtcttatc
      301 cctgaaacag caactatttc aaggcgatta agtagaaagg tgagtaaatg gtcgacattt
      361 actcacccta cagacaacgg cgtattgttt attccgggtt ttgcttacta cttctcagct
      421 cgagatgaag tactgctcat cgccaggtca cgggccttgg gcaacacatc tacggccttt
      481 acaacctggg gatcctcagt cgttatgaaa accctgtcgc ccataattct gccatacgct
      541 gccgtgacga gattgaatcg cagttcgagt tctacaaacg atctattgcg atcaatcatg
      601 gcgtcagaga ccttgtaagc aggatcgttc gacgcaaatg cgcgaaacga cttgtatagg
      661 gcgtcagtga ttttgaactc gtcggattgc agttcacgtc ggaagtcgat gccaccttgt
      721 tggcgaagcg actcaaaacc ggcgacacga ccattaatca attcccgaac aaaggagaag
      781 gttggactca acaacctgcg ttgtgccagg tttacagttc gcgcgtcgac ggtctcatct
      841 ggcgaaatgc cacctccgcc ataaactgca cgaccggtat cggtcttctt ttctggtcca
      901 tttggcttca cgactgattt atcattagcg ccgctgtgtt tgtaatagtc gtaaaaacca
      961 ccattgctgt aatcccgttg aatcaaacga cccgaaggcg tgaagtattt ggcagatgtc
     1021 agtgtcagac cggcaccgta ttccaacgga ataatgcttt ggaccaaccc tttaccaaaa
     1081 cttgtttgac caacgatcaa tgcacggtcg tgatcctgca ttgcaccggc aacgatttcc
     1141 gaagccgacg ctgtgttctc gttaatgaga ataacaaggg gagtcgtgtc tggagagttg
     1201 tttctcgact cataactatt gtcgcgatag ccgttccttc ccttttgcgt caggatcaac
     1261 tgtccattct taaggaagac ttcagcaacg tgtatcgcct gatcaaggaa accgcccggg
     1321 ttgtttctga ggtccagaac cagcgacgtc attccctttg cgtgaaggcg atcaagcgag
     1381 tcaagcaact cgtcggtggt ggtgtagtta aaacctctgg tcatgtcgat gtagccaaca
     1441 ccgggtttca acatgtagga gtcaggaatc gatggttgtg gaacgacgtc acgaactatg
     1501 tcaacttttt cgatcttttt gtccgttgca cgttccagtg tcaggcgaac agttgaaccc
     1561 tttgggccgc gaattttgtc gcgcacctcg gcagagctct tcccactcat attttcgccg
     1621 tttacttcga cgattcgatc gccgtatttg agaccagcac gatgagccgg tgatccgtca
     1681 aaggtggcag taataaaggt gtcagctttg tcgtcgcgga agtaattctg gatggaggcg
     1741 ccgataccga agtattcaga gcgctggtct gttttcagtt cgtcaaattc ttcgcggtca
     1801 taatagttgg aatggggatc cagagaccgc agcatcccga ttattgacga cttaaagacg
     1861 tcgttgtaat tcagtttctt gccgtcaacg tattgatcct gaatgacctt gagagcctcg
     1921 tcgaagtcgt gctcaacgac agccgttgcg gcagtgccag cgtttctggt acttcttcgc
     1981 ccaggaatac ctgagccggt ggttccgctg gcggtcgagc ctgactgtga ctgattcctt
     2041 tgggcgaata cgaatgcggg tacaagtaaa gagagaatta agaagaggag cgctaactga
     2101 gcggagattc ttttcactgc taaacctcgg tgttcttcag atatggccgg gtcggcgagc
     2161 atctactcgc gcctgcaata taacggatgc aaactggacg ggcaaggttg tgcctgatgg
     2221 ccattggact ttggtctttg gtctttgaat ttccaaggca ttgaacctgg agcgctgatt
     2281 gtgaagttgc ccggctctgc aaagaccaaa gcacaaatcc ccagaggcaa agacgttgct
     2341 tgccgtttcg tgtgcccctc cgtagaatga acctaccccc actgtaaatc gtaagacgaa
     2401 cgaaagaatt cgttagccat aagaaggagt ctttacgaaa tgagtagtcc gtatcacaaa
     2461 gaagaacccg gaacgaccga gagtgtcgac gatcttatga ttcgtgacga tgaccccgaa
     2521 gctaattctg aagcccagaa tcgcactgcc tcgtcagtca gggatatcgc atctcgaatt
     2581 ccagagtcgt tgagcgcgat cggacgagat ataagtcgta ctattgagaa agccatctcc
     2641 gcaaaggatg attacgtcgt ggccgttaaa atctctcccg aagcacagga caagcttgaa
     2701 caacttgtac aagccggagt ctttaggagt cgcgctgagg ctgcaggatt tttaattgat
     2761 gaaggtatcc gagtgcagtc ggccctgttc gaacgcgtac gccagaaact tggtgagatc
     2821 gagcgtttac gcgcagaact gcgtggcatg attgctgaca gaccggttta gcggaagtca
     2881 tttcgcccat aactcaggac tcgcaaaagc ggtgctttac tttcctctcg aggggtaaag
     2941 cacgcctttg ttttgacgca aattatataa gacacaactc gtggctccta caattcaact
     3001 accgccaact ccaatcgagg aattcacact cgccaacggc ttaagagtcg tcttgagtca
     3061 agataacgtc attccagtcg tttcgatcgc agtttactac gacgttggct cacggaatga
     3121 aaaggaaggt cgcaccggct ttgcgcatct tttcgagcac atgatgtttc aaggctcgga
     3181 gaatgtgccg aaagctgcgc acttccaatt tatcttcaac gccggcggga ccatgaatgg
     3241 aaccacgtcc accgaacgaa cgaactactt cgaaactctg ccggcaaact atttgcccct
     3301 ggcgttctgg ctcgagagcg atcgaatgag atcgcttaag gttactcaag agaacctcga
     3361 caatcagcgt aatgccgttc aggaagaaaa gcgtttgcgt tacgacaatc agccttacgt
     3421 caacgcgttt ttaagaatga atgaattgat ctttacgaat caggcgaatg ctcattcgac
     3481 aattggttca atggaagatc ttgatgcagc tacgatcgag gacatcaaag agtttttccg
     3541 gatttactac gctcccaata acgccgttct gtcggtagtt ggggattttg atatcgccca
     3601 ggctcacgac cttattgaaa agtattttgg caatattcct cctcaacaaa ctccgccgcc
     3661 ggttgatgtt tctgaattaa gtcacgtcgc cataaagaac gagacttatc acgatccgct
     3721 cgcaccggct cctgcgtttg tcctgggttg gaaaattccc gcgcgtcgga cgaaagattt
     3781 ctatgctcta tcgctggcgg gcagtctact gttcgatggc gatagttcgc ggctttatca
     3841 aaaattcgtt aagggcgacg aatcggttgt atcgatcgaa ggtggagtcg acgagcggcg
     3901 cgggccatca gccttttata tttttgcgct cccgaagccg ggcgaagaag tgggtgatat
     3961 tcagaagaag atctttgcgg agattaaagt cctggcaaag gaaccgccgt cggctgagga
     4021 gatggagaaa ctgcgcaact ctctctcgaa cgacacggtg cgcggacgtc agtcgacaat
     4081 gtatcgcgcg cagcgactgg ccgagtttac gttgtatgac tcaaaaccaa gtctcatcga
     4141 taccgaagta gaccaatacc tgagcattac agctcaggat atcagcgatg ctgtttcacg
     4201 gtatgtcgac gtcgaaaatc gagtggtgct tgatatcatt cctgcggctg ccgaggaaca
     4261 cgaggccgag catgcggcct ctccacaacc accgggcgaa ccgcatcaac catctgcgcc
     4321 accgccacag atcccagaag tgccggcatt agaaccagaa tcacctcgtc atactgatgt
     4381 ggcccagata aaaaccaata cccctacaga gcagccaaag gatcctgcag atgttccgca
     4441 acagtcgggt tccggaccaa tgctttcata gaacatgagt caacaggaaa actttcgtac
     4501 ccaaccgcct ccgccattag caccgcggcc aatagtgatt ccgacgcctc acgagacggt
     4561 cctctcaaat ggattgaccg ttgttgtcgt cgaagataaa cgactgcccc tcgtgagtta
     4621 tcgtctcgct ctggctgtgg gcggtgccta tgatccacca tcgcttcctg gccttactga
     4681 tttacttgcc ggactgttac cggaaggaac tgaatcaaaa acgagtaggc agatagctga
     4741 tgcggtcgcg cggatgggcg ccagcctgtc agccggcgca aattccgact acacgatcgt
     4801 tgcagcatcg gccctcgcgc attttagcga taacgtgctc gagctgatgg ctgacgttat
     4861 cctcaacccc tcttttccgg aaaacgaagt tgacctcgcg cgacaaaaca cgaaggaaag
     4921 tttgagacaa caacgagctc aaccgtcctt tctcgcaagt gagatggtgg ctcgcgtgat
     4981 gtttggcaat catccctatt cagttgtcgc tcctacacct gaatcaatcg atcgctctac
     5041 aagggatgag ttcgtcagtt ttcatcgcgc caagtttgta cctaataatg cggtcttcat
     5101 cgtcgtcggc gatgtcaagt tcgagaagat tctggctcga ttggaggcac tcttttctgg
     5161 ttggaatcga ggagataaat tagctccgaa ttttccggct cctccggttc gcagcagccg
     5221 aaaggcttac cttatcgatc gacccggttc ggctcagtcg aacatcgtga tcgcgaacag
     5281 cggcatcgtt cgaacaagtc cggattactt tccgatgttg ttgatgcaca cggttctggg
     5341 tgcgaccgct tcgtcccgtc tattcatgaa cctgcgagag gagaaaggtt atacatacgg
     5401 cgcatactcg aacctggatg ccaggcgatc ggcgggcacc ttcagagcaa cggcggaagt
     5461 gcgttcagtt gtgactggcg actctttgaa agagttcttt ttcgagttgg agcgtatccg
     5521 taacgataaa gtttcagaga aagagatagc tgatgcgaag tcttatttga ccggggtctt
     5581 tcctgtcaga cttgaaactc aagagggttt gattgatcaa ttggtacaga taaagatgct
     5641 cagcttagcg aaagattatc tcgaaaccta cagagataac gtacaagccg taacgatcga
     5701 ccagatccag cgggttgctc aagcatatgt aaaacccgat caggcagctt tagtagttgt
     5761 tggtgacgga gccacagtac tggatcaaat caagccttat tgtgctgata ttgaaaccta
     5821 caacaccgcc ggaaaattga aagaagttgg ggcagctacg ccagcggaca ttacaggctc
     5881 atgggcaatc aacgttgaca ctccgctggg acagagcata cccgccacat taactatcga
     5941 gcgcaacgat ggaattctga gcggaaggat ttcgtccgaa atgggaaatg ccaaccttgg
     6001 cgaaatcaac ctggaagaca attcgtttca agcccagacg tcgatcgaga tggatggaca
     6061 cgcagttgcc gccgaaatac aggcgcgttt cgagggcgag accctggtcg gaacattaaa
     6121 attgcgccat tcgccgccac tctcatttca cggcgagaag acctcctaag cacaaaaccg
     6181 cccggtgtgg ccgacacacc gggcgaaatc acaggaggaa ccgagtgtta tggtcgtcgc
     6241 tatgcttgcg acaatccgag taacctcaag ctgtaagatg ttattcagct aaacctagtt
     6301 gccacgttgg ctcttaacgc tcgaaacgca acaacgtccc atccccgcct acaacccaac
     6361 cgatcttctt gttgaagttg agcgagtaaa ggttttgctt cgttgatgat tgctgaggca
     6421 gccaggtgcg accttgatct tcagttcgca atatggtgcc attccgacca atcgcccaac
     6481 catcgtcatc attgagaaac tgaacgctta acagcgtcac gctgacgttt gtagctgatt
     6541 ccgtccacgt ttgtccgcca tctgttgttc gcaataacgt cccgcgctct cctacgaccc
     6601 agccgttttt gtcatctctg aaatccacgt gataaagagt gtttgtggta ttgactgttt
     6661 gctttgtcca ggtcagacca ccatcgacgg tattcagtat tgtgccgccg tcgccgacga
     6721 tccatcctcg cctgtcgctt acaaaatcaa tatggatcaa ctccaaacga cttggagc
//
LOCUS       DASSFP010000180         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1532707, whole genome shotgun sequence.
ACCESSION   DASSFP010000180 DASSFP010000000
VERSION     DASSFP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532707"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFH91_03905"
     CDS             complement(<1..479)
                     /locus_tag="VFH91_03905"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET6862159.1"
                     /translation="MPKISDIEETPNPNAVKFILREPISNGVARSYSSAAQVENDPLA
                     RSLFDVGHVVSVFYMDRMITVEKDEEGDWDELLPVLAVPIREAATVAPSAQAAAAAAV
                     GGPLAAVMSDDPRLIRINEILDEKVRPALMGDGGYLEVLGLQDHTLSIRYQGACGSCP
                     "
     gene            796..1983
                     /gene="bshA"
                     /locus_tag="VFH91_03910"
     CDS             796..1983
                     /gene="bshA"
                     /locus_tag="VFH91_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HET6862160.1"
                     /translation="MFIARIDERSVNLGKWAKSLNIGITVYPTYGGSGIVGSELGKEL
                     AERGHTVHFIASSLPTRLTQLNDRVRFHEVEMMSYPLFEHQPYTLALATKMATVAEEE
                     NLDLLHVHYAIPHSISAILARESLKPRRRLPVVTTLHGTDITLVGADRSYLPITRYGI
                     VQSDGVTAISNYLKEATREIFHVDDITVIPNFVCQTDYRRQPVESLRESLSRSGEPLL
                     AHVSNFRPVKRPVDCVEILFRVLQKGIKTRLVMVGDGSERTNVEHRARCLGVYEHCSF
                     VGKQPRIVDYLSVSDVLLLPSEQESFGLAALEAMACEVPVIASRVGGIPEVITDGETG
                     YLSEVGNVDKMAEDAARLLTDQKLRVEMGQRARESAISRYRTDVVIPQYIKFYEDVIS
                     KSG"
     gene            2149..>3246
                     /gene="selB"
                     /locus_tag="VFH91_03915"
     CDS             2149..>3246
                     /gene="selB"
                     /locus_tag="VFH91_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HET6862161.1"
                     /translation="MQHSIIVGTAGHIDHGKSALVRALTGTDPDRLPEEKKRGITIDL
                     GFADLILDDLRIGFVDVPGHERFVKNMLAGAHGIDLVALVIAADEGVMPQTREHFDIC
                     SLLGVQKGIVVITKRDLVDDDMIELVKDEASELVAGSFLENAPIIAVSSKTGLGLDQL
                     TSAVKDVGRQISPRSADFVTRLPIDRAFTIKGFGAVVTGTLISGEIKEGDELELHPTD
                     KKVRVRGAQVHGRTVARAQAGQRTAVNLAGVDVTQLERGMVLSAAGQLRTTQIVDARV
                     DVLKDAPRALKSRARVRVHIGAAEVLARVKILNESTEIAAGKSSLVQLRLESPIIALH
                     GDRFILRSYSPSHTIAGGVVLEPMATKHRAKD"
BASE COUNT          794 a          817 c          845 g          790 t
ORIGIN      
        1 ggacaactgc cgcatgctcc ctggtaacga atactcaagg tgtgatcctg caggccgaga
       61 acttccagat agccgccatc gcccatcaat gctggtcgca ccttctcatc taatatttcg
      121 ttgattctaa tcaagcgcgg atcgtcactc attacggcgg ccaacggtcc gcccactgcc
      181 gcagctgcag cggcttgtgc ggatggcgcc acggtcgcgg cttcgcggat tggaactgct
      241 aagaccggta acaactcgtc ccagtctccc tcttcatcct tttcaaccgt gatcattcgg
      301 tccatgtaaa agaccgaaac cacatgtccc acgtcaaata atgacctcgc cagcggatca
      361 ttttctacct gggccgcgga actataggaa cgcgccacgc cattactgat gggttcacga
      421 aggataaact taacggcatt aggatttggt gtctcttcaa tatcgctgat ctttggcatt
      481 gctaaactct ttctgagtaa ttcaatccga gaaaattaac gattcattat agccgctttc
      541 ggagaacaaa gcagttcacg gggcagttgc caactggagc tctcattacc ccctaaaata
      601 ttcttatgat tagactccga gatgcttgca agatgtcttg ctaaaaagga gggttcaggc
      661 ttatactccc gccctcactt gccccccttc acaaggtgag tcaagttgag atagaggcag
      721 gcgatagcga gggtcgcctg cctcagcttt tttcggcggg atttgggctt tcaacctcat
      781 cctagtattc ttcgcttgtt catcgcacga attgatgaac gcagcgtgaa tctcggaaag
      841 tgggccaaat ccttgaacat aggaataact gtctatccga cctacggtgg tagtggaatt
      901 gttggttcag aattaggaaa agagctcgct gagcgcgggc acaccgtcca cttcatcgcc
      961 tcgtcccttc ctacaaggct aactcaatta aacgaccggg tacgttttca cgaagttgaa
     1021 atgatgtcgt acccgctatt tgaacatcag ccttacacgc tcgcccttgc aaccaagatg
     1081 gcgaccgtgg cggaggaaga gaatctcgat ctgctacatg ttcattacgc tatcccacat
     1141 tccataagcg cgatcctcgc ccgagagtct ctcaaacctc gccgacgttt gccggttgtt
     1201 acaactctcc atggaaccga catcacactc gttggggcag acaggtcata tttgccgata
     1261 actcgttatg gaatcgtgca gtcggacggc gttaccgcga tttcaaacta tcttaaagaa
     1321 gcaacgcgcg agatttttca tgttgacgac attacggtta ttccaaattt tgtctgtcaa
     1381 acggattaca ggcggcagcc ggtggagagt ttgcgcgagt cgctgtcgag gagtggagaa
     1441 cccttattag cgcacgtgtc gaactttaga ccagtcaagc ggccagtgga ttgtgtcgag
     1501 atactcttcc gagttttaca gaagggaatc aagacccgtt tggtgatggt aggcgatggc
     1561 agcgaacgga cgaacgtcga gcatcgcgcg cgatgcctcg gtgtctacga acactgttca
     1621 tttgttggaa agcaaccaag gattgtcgac tatctatcgg taagtgatgt cttgctgtta
     1681 ccgtctgagc aggaatcttt tggtctggca gctctcgaag cgatggcttg tgaggtgcca
     1741 gtaattgcgt ccagagtggg aggcattcct gaagttatca ccgatggcga gactggttat
     1801 cttagcgagg tcgggaacgt cgataagatg gcagaagatg cagcgcgact tctgacggat
     1861 caaaagttga gagttgagat gggccagcgc gcacgcgagt cagccatctc tcgttatcgg
     1921 accgacgtgg ttattccgca gtacataaag ttttatgagg atgttatctc taagagtggc
     1981 tgaagagttt tgattacttt gtactttgtg ctttgtactt tgtgcttcgc gctttgtggt
     2041 tcgtctgtgg gagccaattt taactcgtcg atagaagagc aaagtacaaa gtacaaaaca
     2101 caatacgcaa agcagaagca caactcttag ctgtcgtagg ttcaaacttt gcaacattcg
     2161 attatcgtag gcaccgcagg tcatatcgat catggaaaga gtgcgttagt ccgagctctc
     2221 accggcacgg atcccgatcg tcttccagaa gaaaagaagc gcggcattac cattgatctg
     2281 ggcttcgctg atctcattct tgacgatctg cgcatcgggt tcgtggatgt gcccgggcat
     2341 gaacgcttcg ttaaaaacat gcttgcagga gcgcacggca tcgaccttgt tgctctcgtg
     2401 attgctgctg acgaaggagt gatgccacaa actcgcgagc attttgatat ctgcagcctg
     2461 cttggcgtac aaaagggaat tgttgtcatc accaaacgag acctcgttga cgacgatatg
     2521 atcgagctcg tcaaggatga agcaagcgag ttggtagctg gatcttttct tgaaaacgct
     2581 ccgataatcg cagtcagctc taaaactggc cttggtctgg atcaactcac cagcgcagtc
     2641 aaggatgtcg gtcgacaaat cagcccgcga tccgcagatt ttgtcacacg tttgccgatc
     2701 gaccgtgcgt tcacgatcaa aggcttcgga gctgtcgtta caggcacgct tatatcgggt
     2761 gagatcaaag agggcgatga actggaactt catccaactg acaaaaaggt acgagtcagg
     2821 ggagcgcaag tgcatgggag aactgttgct cgtgcgcagg caggtcaacg aactgccgtt
     2881 aatctcgccg gcgtcgacgt cactcagctg gaacgaggga tggtcctctc agcagctggc
     2941 cagcttcgaa caacccaaat cgtggacgcc agagtcgacg tgttaaagga tgcgccgcga
     3001 gctcttaaat cgcgcgcacg tgtgcgagtg cacatcggcg ctgcggaagt actcgcccgc
     3061 gtcaaaatac tcaacgagtc aactgaaatc gcggccggga aaagtagcct ggtccagttg
     3121 aggcttgaat cgccaatcat tgctcttcac ggagatcgat tcatactccg cagttactca
     3181 ccgtcgcaca caatcgcggg aggcgtcgtg cttgaaccga tggccactaa gcatcgagcg
     3241 aaagat
//
LOCUS       DASSFP010000181         5922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_344906, whole genome shotgun sequence.
ACCESSION   DASSFP010000181 DASSFP010000000
VERSION     DASSFP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344906"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFH91_03920"
     CDS             complement(<1..1181)
                     /locus_tag="VFH91_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862162.1"
                     /translation="MPYELFLALRYLRSRRKRKLARATSIAAILGIALGVGALIIAFA
                     LSNGFRDEMREKILQGTAHISIIPTEFSQQLTKDSVNSLQLTQIDNVSSIAPTTYDGA
                     LVQGQAHSAYAVLRGVEKDHESLIPQRWIQSGFSAPILDNPSDGVPNGVIGNELASRL
                     GVKVGETIQVIPANDFTLSGTRPIRKIRVAGIFRSGLFEYDSTWIYVSLDTANVLSNN
                     SHSGTVISVQVKDIDNVSQTADKIRRLLGNSFTVIDWQQANQQLFSALALERRMGLFI
                     IGLIIAIASLNITSMLILVVVERRRDIAILNALGASRNGVMLLFVIEGAIVGVIGAVA
                     GLALGLAGCVLGNYFKIVSLPADVYSISSVPLNVHLSEAFLSALVAIVLSVVATIYPA
                     RA"
     gene            complement(1190..2800)
                     /locus_tag="VFH91_03925"
     CDS             complement(1190..2800)
                     /locus_tag="VFH91_03925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6862163.1"
                     /translation="MIQTYLTTSTPETVQPDEAATKSFWRLWPWWLVPSLIALALVLY
                     FVDPFIGDWDGIDYTIHSVAGYPSSMALGRNLFIFTNHTLFVIARKLLGLQPEHAYLL
                     FKYAVVAQVPFAVIACWILARSVSKSVYTATTAALLVVFSPVFVLYAGQVMTDVPSVL
                     LLTVAIIIHLKGVTESRVWLVIAGAAILGLGVNLRETVGFYLPWLLLSPFALGWKLRR
                     REILFVGLSCLVFLILAFGWFGYWMLSDPQYRSLWHGWRESMREESSRHPVSITNLLP
                     FIVYLFITAPLVFLTLPFGPWLEWRSRRLTPMLLLWLVAFVADLLLFFNYSTTVNWRY
                     FLTGLPGLAPLGAHWLLRLMAIPLRTLRLAFVVCVFFIVSLATTFGLFFRPVSYEFIE
                     RRAMSKEYKHRLAQLPQDAVMISGAQTIAVTYWASIGAGRWETIGTGGGWPGDKLFSI
                     IDGYLSQGRRVFIDADPRWWLVCGWQRDEIPVVVSLERHFRFRRVSETIFELRPLTDT
                     EANDNPDLKRLLPENRPQDGKKCTPGLT"
     gene            complement(2766..3608)
                     /locus_tag="VFH91_03930"
     CDS             complement(2766..3608)
                     /locus_tag="VFH91_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862164.1"
                     /translation="MEKRRILVVDNNDELRALLEQVLGRLGHDVVVTGERENALARED
                     LDQFDLVISDLSEAEVNGQPIGDLQRKHLVTPVTPPQSGIIKAFKMGAANYLRLPYNR
                     EELREIVEKTLSYKLRYVDDPELLPHVHEKIEFELPSDLALLNGVLEYLQERVSKLGL
                     IKPERSNLFIALDEAFVNAVKHGNKNDPTKLLRITAELSPREASFTVEDEGEGFDIRE
                     IPDPCDPANLFRTSGRGVLLIYNIMDEVEYNAQGNRVKMIKRPELPVEAQLVDPNVPD
                     DKHA"
     gene            3811..4728
                     /locus_tag="VFH91_03935"
     CDS             3811..4728
                     /locus_tag="VFH91_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6862165.1"
                     /translation="MAKQGKIKTNLEYLAARSVLAVFGLLPASVAMASGRMLGRLAYF
                     FATNLRKTGLRNLELAFPEKSLEERRAILKGCFLNLGRVLGVFSQLSTRSADSLRDLI
                     APEGLNHLENAKAEGKGLILFTAHLGAWELTSFGLSLIGHPISFLVRRIDNPEVEKLV
                     DDVRMRFGNQTLDKLAAARSMVKILRSGQVLGLLLDLNTLDDEGIFVDFFGVPASTNF
                     ITAKLALRTGSAIIPVFAPWDDRRKKFLLQVQPRVSTELSGDEDTDVRRLTEKLSLII
                     ENEIRNYPDQWLWIHRRWKTRPPGEASIY"
     gene            4754..5512
                     /locus_tag="VFH91_03940"
     CDS             4754..5512
                     /locus_tag="VFH91_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6862166.1"
                     /translation="MEISAKEKLPDHRSIDERVKKAYEFADLSDYSFKDRMMIRAADL
                     AFYFLITFIGWSSRIEVEGWSNHEAASSNQGLPIYTFWHDRIFMMTYFWRNRKIVVMT
                     SRSFDGEYIARFIQRFGYGAARGSSTRGGVGAIVEMTRLMRAGCATAFTIDGPKGPRH
                     LAKMGAVLLAKKSGHPIVPMNVSVQKFWQAPSWDFFQIPKPFTTARVFLAAPIYVPPD
                     ADDTVLKAKRDELQSVLDDLHRKGEEWRQSKETL"
     gene            5627..>5922
                     /locus_tag="VFH91_03945"
     CDS             5627..>5922
                     /locus_tag="VFH91_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862167.1"
                     /translation="MKSILSFALCLVFVATSQAQTQLKPPTESAMDRFLRYVKIDTQS
                     QEDQTTVPSTRKQLNLANLLAKELTALGTENVRVSEFGIVYATVPGNLPDNSKVP"
BASE COUNT         1588 a         1481 c         1490 g         1363 t
ORIGIN      
        1 gctcgagcgg gatagatagt ggcaactacg ctcagcacta tagctacaag ggccgataaa
       61 aatgcctcac tcaaatggac gtttagcggg acactgctga ttgagtagac gtccgccggc
      121 aagctaacga tcttgaaata atttcccagg acacaaccag caagtccaag tgcgagtcct
      181 gctactgcgc caatcacccc aacgatcgcg ccttcaatca caaataaaag catcacacca
      241 ttacgactcg cgccaagtgc attaaggatc gcaatgtctc gtcgtcgttc gacaaccacc
      301 agtattaaca tgctcgttat gttgagggat gcaattgcaa taattaaacc aattatgaaa
      361 aggcccattc ttcgctcaag cgcgagtgca gagaacagct gctggtttgc ttgttgccaa
      421 tcgataaccg tgaaagagtt gccaagcagt ctccgaatct tatcggctgt ttgactcacg
      481 ttgtcgatgt ctttgacttg cacgctgatg acagttccag aatgactatt attcgagagt
      541 acgttggcgg tatccagcga gacatagatc caggttgagt cgtactcaaa tagtcctgat
      601 cgaaagatcc cggcaacgcg aatcttcctg atcggtcgag ttcccgaaag agtgaagtcg
      661 ttggctggaa tgacttgaat agtctcgcca accttcacac ccaatctcga ggccaactca
      721 tttccgatca ctccgtttgg tacgccgtcg ctggggttgt ctaaaatggg agccgagaaa
      781 ccggactgga tccaacgttg gggaataaga gattcatggt ctttctcaac acctcgaagt
      841 acagcgtacg cagagtgggc ctgaccctgg accagcgccc catcataagt cgttggagca
      901 atgctcgaca cgttgtctat ttgtgtcagt tgcagggagt tgactgaatc tttagtgagt
      961 tgttgagaaa actctgtcgg gatgattgag atgtgtgcag ttccctgcag gattttctct
     1021 cgcatctcat cgcgaaaacc attcgagagt gcgaaagcta tgatcaatgc acccacgcct
     1081 aacgcaatgc ccagaatcgc tgcgatcgaa gtggccctgg caagctttcg ttttcgtctt
     1141 gatcgcaagt agcgcagcgc gagaaataat tcgtaaggca ttctatttat caagttaggc
     1201 ctggcgtaca ctttttgcca tcttgaggcc tattctcagg taataacctc ttcaaatcag
     1261 gattgtcgtt tgcttcggtg tcagtcagcg gtcgtaactc gaagatcgtt tcagaaaccc
     1321 ggcgaaacct aaaatggcgc tcgagcgata ctacaacagg gatctcatcg cgctgccagc
     1381 cgcaaaccaa ccaccatctt ggatcggcgt cgatgaaaac acggcgaccc tgactcaagt
     1441 aaccgtcgat gatcgaaaat agcttgtcgc cgggccaacc tccaccagta ccaattgtct
     1501 cccaacgacc ggcgccaatc gaagcccaat acgtcacggc tattgtttgg gctcctgaaa
     1561 tcatcacggc atcttgcgga agctgagcca gacgatgctt gtattcctta ctcatggccc
     1621 gacgttcgat aaattcgtag cttactggcc ggaagaaaag tccgaacgtt gttgccaggc
     1681 tcacaatgaa aaacacgcaa acaacgaaag cgagccttaa ggtgcgaagc ggtatcgcca
     1741 ttagccggag caaccaatgg gcaccaagcg gcgcaagtcc gggtagaccc gtcaaaaagt
     1801 atctccagtt gaccgtggtg ctgtaattga aaaacaaaag cagatctgcc acgaacgcga
     1861 ccaaccaaag caggagcatt ggcgtcaatc gacgcgaacg ccattcaagc catggaccaa
     1921 aaggcagtgt aagaaaaacg agtggcgcag taataaacag gtagacaatg aatggcaaca
     1981 gattggtgat ggatacagga tggcgactgg actcttcacg catcgactcg cgccatccat
     2041 gccataacga acggtattgg ggatccgaca gcatccaata cccaaaccaa ccgaacgcca
     2101 aaatcaagaa cactaagcac gaaagaccaa cgaacaagat ttcccgacgc ctcagcttcc
     2161 aaccaagcgc aaaaggcgac aataaaagcc acggaagata gaaaccgact gtctctcgca
     2221 agttgacacc aagtcccaat attgccgcac cagctattac tagccagacc cggctttcag
     2281 tgactccctt gagatgaata atgatcgcaa cagtgagcaa gagaacagag ggaacatccg
     2341 tcattacctg tccggcgtaa agtacgaaga ccggagaaaa gactacaagc aacgcagctg
     2401 tagtagcggt atacacagac ttgctgactg accgagcgag aatccaacac gcaattactg
     2461 cgaacggaac ctgggccacc actgcgtatt tgaagagcag gtatgcatgc tcgggctgta
     2521 ggccgaggag ctttcgtgca atcacaaaca gagtatggtt ggtaaagata aaaaggttgc
     2581 gaccgagtgc catcgatgag ggataaccgg cgactgagtg gattgtgtag tcaataccgt
     2641 cccaatcgcc gatgaaggga tcgacaaaat agagcacaag ggcaagcgct atcagggatg
     2701 gcacgagcca ccagggccat aaacgccaga aggatttagt ggcagcttcg tccggttgga
     2761 ctgtttcagg cgtgcttgtc gtcaggtacg tttggatcaa cgagttgagc ttcgaccggt
     2821 agttctggac gcttaatcat tttcacgcgg ttaccctgcg cattgtactc aacctcgtcc
     2881 atgatgttgt agataaggag aacgcctcga cccgaggttc tgaagaggtt tgccggatcg
     2941 cacggatctg ggatctcacg aatgtcaaag ccttcacctt catcctctac ggtaaagctt
     3001 gcttcgcgcg gcgagagctc ggctgtgatt cgaagcaatt ttgttggatc gttcttatta
     3061 ccgtgtttga cagcattcac gaatgcttcg tcgagtgcaa taaagagatt cgatctttca
     3121 ggtttaatga gtccgagttt tgacactcgc tcctgaagat actcaagaac gccattcaat
     3181 agcgcaaggt cgctaggcaa ctcgaattcg atcttttcgt gaacatgggg cagcaactcg
     3241 ggatcgtcca catatcgcag cttgtacgaa agagtttttt cgactatttc ccgtaattct
     3301 tcacggttgt agggcagcct gaggtaattg gctgcaccca tcttaaaagc cttaataatt
     3361 ccggactgtg gcggcgtaac gggagtgacc agatgtttac gctgcagatc accaatcggc
     3421 tgtccattaa cttcggcttc ggaaaggtca ctgataacga ggtcgaactg gtcgaggtct
     3481 tcgcgcgcga gggcattctc ccgttccccg gtaacgacaa cgtcgtggcc aagcctgccg
     3541 agaacttgtt ccaggagagc acgtaactca tcgttgttat cgacgacgag aatccggcgt
     3601 ttttccatcc aactccaatc caatcgaggt gttgaggaga gtcacagccg cacacgaagg
     3661 gcgcaataca aagcactgta cgaaaatctt tcgcgcctcg ttcgcgaaat atatcagaaa
     3721 cctgcacaac ccaacagacg ggccgggtta gatggcagca gtcgctcgcc gggggcatag
     3781 cttgttaaac tacgccaccc atcctgctca atggctaaac aagggaagat aaaaacaaat
     3841 ctagagtatc ttgcggcccg ttcggtgctc gctgtatttg gcttgctccc cgcatcagtc
     3901 gcaatggcat caggcagaat gctgggcaga ctggcttatt tcttcgcaac aaatttgcgc
     3961 aaaaccggcc tccggaatct ggaattggca tttccggaga aaagcctgga agaacgccga
     4021 gcaattttaa aaggatgctt tttgaatctg gggcgagtcc tgggagtgtt tagccaactc
     4081 tcgacccggt cggcggattc tcttcgtgat ctgattgctc ctgagggttt gaaccatctg
     4141 gaaaatgcaa aagctgaggg caaagggctg attctcttca cggcacattt gggagcctgg
     4201 gaactaacga gctttggact ttcgcttatt ggacatccaa ttagtttcct ggtgaggcga
     4261 attgacaatc ccgaagtgga aaaactcgtg gatgatgttc ggatgagatt tggaaaccag
     4321 acattggaca aactcgccgc ggctagatcg atggttaaga tcttgcgctc cggacaggtg
     4381 cttggactct tattagactt aaacactctc gacgacgaag gaatttttgt cgactttttc
     4441 ggagttccag cttccaccaa tttcatcacg gcaaagcttg cgctgcgaac cggatctgca
     4501 atcatccctg tctttgctcc ctgggacgac cggcgcaaga agtttttatt gcaagtacaa
     4561 cccagggtct ccaccgaact tagcggtgat gaggacaccg atgtccgccg gttaacggaa
     4621 aaactttcgc tgatcatcga aaatgagata aggaattatc cagaccagtg gctctggatt
     4681 cacagaagat ggaaaactcg acctcctgga gaagcaagca tctactgatg gttcgatccc
     4741 ttctctaacg tgtatggaaa tctccgcgaa agaaaaactg cccgaccacc gttctattga
     4801 tgagcgggtc aagaaagcct acgaattcgc ggatctgtcc gattactcct tcaaggatcg
     4861 aatgatgatt cgcgccgccg atctggcctt ttatttcttg atcaccttta tcgggtggtc
     4921 gtcacgaatt gaagttgaag gctggagcaa tcacgaggca gcgagtagca accagggtct
     4981 gccaatctac acattctggc atgatcgaat tttcatgatg acgtactttt ggcgaaatcg
     5041 aaagatcgtt gtaatgactt ctcgcagttt tgatggtgag tatatcgcga gattcatcca
     5101 acgatttggc tatggcgccg caagaggctc aagcactcgt ggaggtgttg gagcgattgt
     5161 ggaaatgact agattgatgc gagcgggctg cgctacagcg tttacgatcg atggtccaaa
     5221 gggaccgagg catctcgcga agatgggcgc cgtgctactg gcaaagaaat ccggccatcc
     5281 gatcgtgccg atgaatgtgt cggtgcaaaa attctggcag gctccaagtt gggacttctt
     5341 tcaaattccg aagccattta caactgcgcg agtgtttctg gcagctccca tttatgttcc
     5401 gcccgatgcg gacgatacag tattgaaagc gaaacgcgat gaacttcaaa gcgtactcga
     5461 cgatttgcac cgcaaaggtg aagaatggcg acagtctaaa gagactctgt aaatagtcgg
     5521 gtggtcacag aggtcattat ctccctacaa cttcagcggg attttgcgca tcgacttaac
     5581 gttatacccg ctctcctgct caaaaccctg tcgaaaggct tttcggatga agtcaatatt
     5641 gtcatttgcc ctgtgtcttg tgttcgtcgc tacaagccag gcacaaactc aacttaaacc
     5701 accaacagaa tcagcaatgg atcggtttct gcggtacgtt aagatcgata cgcagtcaca
     5761 ggaagatcag acgaccgtcc cgagcaccag aaagcagctc aacctcgcga atctacttgc
     5821 gaaagaactg acagcgctgg gaactgaaaa cgtgcgcgtc agtgagtttg gaattgtgta
     5881 cgcaacggtg ccgggcaatc ttccagacaa ttctaaagtc cc
//
LOCUS       DASSFP010000182         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_197486, whole genome shotgun sequence.
ACCESSION   DASSFP010000182 DASSFP010000000
VERSION     DASSFP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197486"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="VFH91_03950"
     CDS             complement(<1..2038)
                     /locus_tag="VFH91_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6862168.1"
                     /translation="MSQNKRELLPEQAAAAYEIKSHVSVTAGPGSGKTTVLVERYLHI
                     LRQHKLSIDQIVAITFTNRAANEMRERLRSELNAILRSAEPNERRHWLSYKRTLDGAV
                     ITTIHGFCARLLKEFPIEARVDPQFLLLDEHRAAMLLDSVVEEVLSEFITKGHVEISR
                     LTLGIGRSRLAAALAQLYREVRGQGIPLEKLALKTGEVHAKEADLHLSLKELEAAMAA
                     FLAVRRTTEKSKANQAEVSSAWPRLREFLKSIPEHDTLADYCQTIDGFRTLRPGLRAP
                     VVEQVKVLDELVWEKELGGRIPQICLDLFAGTYALELVSVLTRIHERLNEEKYKLSAL
                     DFDDLETRTIQLLELPEVIARVSERYKFFLVDEFQDTNALQQTLLERLALSRRQKPAN
                     LFIVGDRKQSVYGFRGADVDVFREMTSILTAAGGEEIPLRLNFRSQPPLIHFFNLLFA
                     RLFRVPDDSSREEVKNLGYVEHEDSEPKRETRDAGPLVELLVSTNPPAEEDQEADTDS
                     RVLDASQVARRINSLMQSTPSLKYSDFALLFRAMTNVQIYESTFRRANIPYQTVLGRG
                     FYDREEISDLIQLLRFLDNKTDELALAAVLRSPLCGLSDNALLALRCAPKTENGSTDP
                     LRSVAITRSLFTALRQHQSISFISIEEHELLDRAANLIQQLIARRHHYTIEQLLR"
     gene            complement(2041..>2910)
                     /locus_tag="VFH91_03955"
     CDS             complement(2041..>2910)
                     /locus_tag="VFH91_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6862169.1"
                     /translation="ALDLQAIDAGKLLHDILRRFFEGYRRTPLYQLDQAVLRKELAEI
                     ADKVFDEHERVVPPLNRQIWKIDREIRKILLDQVLLYEIGLQQEASANSVLPAYFEVA
                     FGNPRFSSRDPGSTSDPLELIRDTFVGTETIKINGQIDRVDVAADNTFIAYDYKLSTG
                     QSKDDIRSGRSLQIPIYLEALEKLVLPGYSNAGGGYYIIRGSSDRRNRGIHRKSKIQY
                     SQINPRVNALLSDEEWEQVRREAIEKIWQFLDGMRSGRFLVNPSERIKTCRFCDFTAV
                     CRYDRYRIDKKKN"
BASE COUNT          701 a          738 c          724 g          747 t
ORIGIN      
        1 atcgaagcaa ctgttcgatc gtgtaatgat ggcgcctggc gatcaattgt tgaataagat
       61 tcgctgctcg atcgagtagt tcatgttcct caatactgat gaaggaaatt gactgatgct
      121 gtcgcaaggc cgtgaacaga cttcgcgtta ttgctacgct acgaagggga tcggtcgagc
      181 cattttctgt cttcggcgca caacgaagtg cgagcagtgc gttgtctgat agaccacaaa
      241 gaggcgaccg gagtacggcg gccagagcca actcatcagt tttattatcc agaaaccgta
      301 gcagctggat caggtcgctg atctcctctc ggtcataaaa gcccctcccc agcaccgttt
      361 gatacggaat gttcgcgcgc cggaaggttg actcatagat ctgcacgtta gtcattgcgc
      421 gaaacaaaag tgcgaagtcg ctgtacttca ggcttggagt tgactgcatc aacgaattga
      481 tgcgacgtgc gacttgtgac gcatcaagta ctcgagaatc cgtatcagct tcctgatctt
      541 cttcagcagg tggatttgta ctcaccaaca actcaacgag cggaccagcg tcacgagttt
      601 cgcgttttgg ctcgctgtcc tcatgctcga catatccaag attcttaact tcttcccgcg
      661 acgaatcatc gggaacacga aatagtctgg cgaagagcag attgaaaaag tgaatgagtg
      721 gcggttgact gcgaaagttg agtcgtagag gtatctcctc accacctgcg gcggtcagta
      781 tcgatgtcat ctctctgaag acatctacat ctgcaccgcg aaaaccgtag accgactgtt
      841 tgcgatcgcc gacaataaac aggttggccg gcttctgtct tctcgatagc gccagtcttt
      901 cgagcagcgt ttgttgtaaa gcattcgtat cctgaaattc gtctaccagg aagaacttat
      961 aacgttccga tactcgcgca atcacttcag gcaactccag cagttggata gtgcgagttt
     1021 caagatcatc aaaatccaga gccgaaagct tgtacttctc ttcgttcagg cgttcgtgaa
     1081 tacgggtcag gacactcacc agttcaagcg cgtaagtgcc ggcgaagaga tcgagacaaa
     1141 tttgtggaat gcggcctccc aactcttttt cccagacaag ctcgtccagc accttcacct
     1201 gctcaacaac gggcgctctt aggccgggcc gtagagttcg aaatccatcg attgtctggc
     1261 aatagtcggc aagggtgtca tgttcgggaa tgctcttaag aaactctcgt aacctgggcc
     1321 atgcgctact gacttcggct tgattcgcct tgctcttttc ggtggttcta cggaccgcta
     1381 gaaatgcggc catcgctgcc tcaagttctt taagtgaaag gtgtaggtca gcctcttttg
     1441 cgtgaacttc tcctgtcttt agcgctagtt tctcgagcgg aatgccctga ccgcgtactt
     1501 ctcgataaag ctgggccaaa gcggcggcca ggcggctgcg gccaattccg agcgtaagtc
     1561 gagagatctc tacatgaccc ttagtgatga attctgagag tacttcctct acgactgaat
     1621 cgagcagcat cgcagcccga tgttcatcga gcagtagaaa ctgtggatca actcgcgctt
     1681 caatcggaaa ttcctttagc aagcgcgcac aaaacccgtg aatagttgtt atgaccgctc
     1741 cgtcaagcgt gcgcttgtaa ctgagccagt gccgccgttc gtttggttct gcacttctga
     1801 gaattgcgtt gagctcggag cgtaaacgct cacgcatttc gtttgcggcc cggtttgtga
     1861 aagtgatcgc aacaatctga tcgattgata gtttgtgttg ccgcagaatg tggagataac
     1921 gctcaaccag tacagtcgtt tttcccgaac ctggaccagc cgtaactgat acatggcttt
     1981 taatttcgta cgcggctgct gcctgttccg gcaaaagctc acgcttattt tgcgacatgc
     2041 tcaattcttc ttcttgtcga ttcgataccg atcgtagcga cagaccgcag tgaaatcaca
     2101 aaatcgacac gtcttaatac gctcagatgg atttaccagg aaccgtccac ttcgcattcc
     2161 atccaggaac tgccagattt tttcgattgc ttcgcgacgg acctgttccc attcttcatc
     2221 gctgagtaaa gcgttcaccc ggggattgat ctgcgaatac tggatcttac tcttacgatg
     2281 gataccgcga ttgcgacggt cgctcgaacc tctaatgatg tagtaaccgc caccagcatt
     2341 tgaatatccc ggcaaaacca atttctcgag cgcttcaagg tagatgggaa tctgtagact
     2401 tcttccagaa cgaatgtcgt ctttactctg acccgtggag agtttgtagt cgtaggcaat
     2461 gaaagtgtta tcagcggcga catcaacgcg atcgatctgc ccgttgattt ttatcgtttc
     2521 ggtgccgacg aaagtatcgc ggatcagctc gagggggtcg gaagttgatc ctggatccct
     2581 ggacgaaaag cgcggattgc caaaagcgac ttcaaaatat gcgggtaata cactgttcgc
     2641 cgacgcttct tgctgaagac caatttcgta aagcaggacc tggtccagca aaatcttccg
     2701 tatctcgcga tcaatcttcc agatttgtcg attcaaaggc ggaacaactc gctcatgttc
     2761 atcaaaaacc ttgtccgcta tttcagcgag ttctttgcga agaacggctt gatcgagttg
     2821 atacaagggg gtacgacgat agccttcaaa gaaacgacgc aatatatcgt gaaggagttt
     2881 gccggcgtca atcgcttgca ggtcgagtgc
//
LOCUS       DASSFP010000183         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1235821, whole genome shotgun sequence.
ACCESSION   DASSFP010000183 DASSFP010000000
VERSION     DASSFP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235821"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFH91_03960"
     CDS             <1..725
                     /locus_tag="VFH91_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862170.1"
                     /translation="IAFSPLFPLALFALFTPPGKEWRDFNNYSFVYSVIYNVLMLRTL
                     IFFGSAWIFMNLFRGELVDRSLHYYFLSAVRREVLVVGKYLSGLITAVVLFTGTTVVS
                     LLLLYFPRFYSQSVRYFTEGQGFKQLLTYAGITILACVGYGAFFLVVGLFVRNPIIPA
                     ILLYGWEWLNFLLPPLLKKISVIHYLNSLAPVPVSEGPFAVVAEPTPAWIAVPSMMLV
                     TLIVLAIAGRRIRRMEVRYGSD"
     gene            complement(870..1487)
                     /locus_tag="VFH91_03965"
     CDS             complement(870..1487)
                     /locus_tag="VFH91_03965"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6862171.1"
                     /translation="MVKLIKPTKRKLSREFYTRSNVLLVARELLGKLLVVPHESGKRV
                     SGMIVEVEAYRGPEDRASHAYGGRRTRRTETMYQTGGVAYVYFVYGMYNQFNVVTNVQ
                     DIPHAILVRALEPVEGLELMRSRRHLHPDRNLTNGPGKLCIAMGIDRQLDEADLLGDR
                     VWIEDYLTVSRGRILKGPRVGIDYAEEWVNAPWRFWIKGNQYVSK"
     gene            complement(1480..>2163)
                     /locus_tag="VFH91_03970"
     CDS             complement(1480..>2163)
                     /locus_tag="VFH91_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6862172.1"
                     /translation="FPLLIWLGLDARVANGTSTVALWPGLFGGLYGYRKELENSSTIL
                     IRLGGTSIVAGAVGAWLLIRTPSPVFARLVPFLILFATILFMSQSAINQRFKFGKLDE
                     QPHAGWWIGAMVFQFFSAMYGGYFGAGNGILMLAALGLLGLNELNRANGIKNFLGICI
                     NSIAVLMFSLNGLVVWQDSLVMACGALAGGYFGARMAVRVGQRWVRRGVVIIGLVIFI
                     VMVWNLRHG"
BASE COUNT          520 a          568 c          519 g          556 t
ORIGIN      
        1 tgattgcctt ctcgccgctc tttccgctcg cgctgttcgc gctgttcacg ccgccaggaa
       61 aagagtggcg agatttcaat aattacagct tcgtctactc ggtcatttac aacgttctga
      121 tgctgcgcac gctgatcttc ttcggctcgg cgtggatctt catgaacctg tttcgcggtg
      181 agctcgtcga ccgcagtttg cactattact ttttgagtgc agtccgacgt gaagtgttag
      241 tggttgggaa gtacttgtca ggtctgatta ctgcggtcgt gctgtttacc ggtacgacgg
      301 tcgtgtcgct gctacttctt tattttcccc ggttctactc tcaaagcgtc cggtacttca
      361 ctgagggaca gggcttcaag caactcctga catatgcagg tattaccatt cttgcttgcg
      421 tcggttatgg agcgtttttt ctagtcgttg gtctattcgt tcgtaatccg attattccgg
      481 cgatattact ttacggctgg gaatggctga atttcctgct gccgccatta ttgaagaaga
      541 tcagtgtcat ccattatttg aattcattgg cgccggttcc ggtttcggaa ggaccattcg
      601 cagtcgtggc agaacctacg cctgcgtgga tagctgtgcc aagcatgatg ttggtcacgc
      661 tgatcgttct tgcgattgca ggtcgtagga ttaggcgaat ggaagtcagg tacgggagtg
      721 actaaagcag ggggcagggg ccagttcaag gaaagtctat tggagaatta tgaattgata
      781 tttgataatt cctattttga tctgggcctc gcgtaaacaa atcagcaatt ctcaaatatc
      841 aattcataat tatccaatac tgcccctcgt cacttactca catactgatt acccttgatc
      901 caaaatctcc agggggcgtt cacccactcc tcggcgtaat caattcccac tcgaggtcct
      961 ttcaggatcc gacctctcga aacggttaga taatcttcaa tccaaactct gtcacctaac
     1021 agatcggcct cgtccagctg acgatcgatg cccatcgcga tacaaagttt tcctgggccg
     1081 ttagtcaaat tgcgatcggg atgaagatgt cgacgcgacc tcattaactc gaggccttct
     1141 accggttcca gcgctcgcac cagaattgca tgcggaatgt cctgtacatt agtgaccaca
     1201 ttgaactggt tgtacattcc atagacaaag tatacgtagg caacgccacc agtctgatac
     1261 atcgtttcgg tcctgcgagt gcggcggccg ccgtaagcat gtgaggcccg gtcttctggc
     1321 cctcgatagg cctcaacctc aacgatcatg ccggaaacac gctttcccga ttcgtgaggt
     1381 acgacgagaa gttttcccaa taattcgcgt gcgacaagca aaacatttga gcgggtgtaa
     1441 aattctctcg ataacttgcg ctttgttggc ttgatgagct taaccatgtc tcaagttcca
     1501 aaccatgaca ataaagatca caagtccaat gattaccaca cctcgccgga cccatcgttg
     1561 gcccacgcgc accgccattc gcgctccgaa atagccgccg gccaacgccc cgcacgccat
     1621 cacaagagaa tcttgccaaa ctaccaggcc attaagtgaa aacatcaaca cggcgatgct
     1681 attaatgcag attcccagaa agttcttgat gccatttgcg cgatttagtt cattcaggcc
     1741 taagagtccg agggccgcaa gcatcaggat gccattgccg gctccaaaat atcctccata
     1801 catcgcagag aaaaactgga acaccattgc gccgatccac caacccgcat ggggttgctc
     1861 atcaagcttt ccaaatttga atcgttgatt gatcgcgctc tgcgacataa agagtattgt
     1921 tgcaaagaga atgaggaagg gaactagtct ggcgaagacc ggggaaggag tgcgaattaa
     1981 cagccatgct ccaacagccc cggcaacgat gctcgtgccg ccaagccgga tcagaattgt
     2041 cgaactgttc tccagctctt tacggtatcc gtagagacca ccaaatagac cgggccagag
     2101 agctacggtg ctggtaccgt ttgcgactct ggcatccaga cctaaccaga ttagtagggg
     2161 aaa
//
LOCUS       DASSFP010000184        11294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1378884, whole genome shotgun sequence.
ACCESSION   DASSFP010000184 DASSFP010000000
VERSION     DASSFP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378884"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..958)
                     /locus_tag="VFH91_03975"
     CDS             complement(302..958)
                     /locus_tag="VFH91_03975"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET6862173.1"
                     /translation="MRRLLFLSLAAIVAVSCLVFSSSAENVSLRGDYVEVRTASVFAG
                     ACHYNGEVTTTGRDAMMAWSVTSGKWQGVDLTGVRAVAVVSSDANLAESDAARQSEIL
                     IDNSVSRTQALAFLHALEEKYAAALGKVVAVRYTPVSFDHQDKSFNVVAGEATINVQA
                     MPNDLCCKMPNLVWYKPLVGLENRKVGYTLNANYSGKSVGDPWGRSGENSAFYGTFSL
                     "
     gene            complement(991..1227)
                     /locus_tag="VFH91_03980"
     CDS             complement(991..1227)
                     /locus_tag="VFH91_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862174.1"
                     /translation="MANEANSSPVQSPYCGSLRSKKFFMRDQLATSADDYIDSANHVW
                     CCETQEVIGPDNNRVEPDKCGPGRSCYSSAIKSV"
     gene            complement(1294..2295)
                     /locus_tag="VFH91_03985"
     CDS             complement(1294..2295)
                     /locus_tag="VFH91_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6862175.1"
                     /translation="MKAFEWTNPKDINEAVKLLSTTAPTDIDEAPRPIAGGQDLLTTM
                     KDYTSRPARLVNLKNLRGMNRLSLDSKGLTIGALITLNELEEHRGIRKVMPGLAEAAH
                     SIATPQIRNLGTVGGNLCQRPRCWYFRLEEVVCLKKGGSECYAATGENKYNAIIGGGP
                     SYIVHPSDLAPMLLALGARVTVVGAEGKRVIPLDKFFTLPSEGNIRRENVLKNDDIIT
                     EVFVPASPLTEKSTYLKFKERESLDFAMASVAAAVQFDSGKKVKDVRLVLGGVAPIPW
                     RVPAAEKFLIGKTLTPDVLGEAGKIALSEAKPLEKNAYKVPLTQTLVRRALTKTAGI"
     gene            complement(2402..4612)
                     /locus_tag="VFH91_03990"
     CDS             complement(2402..4612)
                     /locus_tag="VFH91_03990"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6862176.1"
                     /translation="MQQAPAKKKKKIKVPRVINGIETMVEIEVDEDSGPGWGPNDKHT
                     LLNHRMTRVDGPLKTTGAAKYTYDQRLPGMLYARILRSPHAHARIKSLNTDAASKIPG
                     VKAIIPAPLTEVRFAGAPVAAVAATTPDVAADALRAIKVEYEVLPHVVHATDAIKPDA
                     PKVVDEPNESNLQQKNKNGDPAKAEAAFATADAIVEAEYISPRIHHACLETHGMVVDY
                     RGGDTATIYASTQGTFTIPEDAAKELGLPQSAVTAIVQHMGGGFGSKFGIGTEGMFAC
                     RLSKQAKAPVKLMFTRYDEFVMAGNRSASWQKLKAGVKNDGTIVALQARQYRLGGIGQ
                     GSQAGQPYIYRMGDTYREVYALHTNEDSSVAMRAPGHPQSSFAVESLVDELAYKIKMD
                     PVAFRKKNLRDVVYHRQLDRGAREIGWDKRNPVAGASAGPVKRGMGCAVGTWGGGGNN
                     QCKVEVTISRDGSVLVAVGTQDLGTGTRTFTRAIVAEEFGLQVNDVREEIGSSKLGGA
                     NSSGGSTTAASLSPSVKDGAIKARLLFSEKVAPLLDNAKPEEITFSGGQVSAKGKSLA
                     WRQACAALPAAGITGRGEWRQDLQASGIHGASFAEVEVDVETGHIRPIKLVHVQDGGL
                     PLNRLTLESQINGGMIQSLGMALWEGRVMDAQLGLQLNPGFGDYKLPGALEMPELVPI
                     IDDEDKREAVIGIAEGCIIPALGAVVNAVFNACGVRVRELPITPDKILMGQMNT"
     gene            complement(4638..5282)
                     /locus_tag="VFH91_03995"
     CDS             complement(4638..5282)
                     /locus_tag="VFH91_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6862177.1"
                     /translation="MAKDEDDKDKELAHDPSRRKFLKGVGIASAGAALVDKLSLEIEA
                     QEKIAPATTLSGTVSLTLDINGQKRSVQVEPRTTLLSTIRDYLEPAITGPKLVCDVGT
                     CGACTVLLDGKPAYSCLVLAVDATNKKITTVEGLGTPEKPNAVQAAFVEHDALMCGFC
                     TPGFVTTISAYLKKNPNPSLAEVREACKGNFCRCGTYPRVFEAALAAAKSGKTV"
     gene            5561..6796
                     /locus_tag="VFH91_04000"
     CDS             5561..6796
                     /locus_tag="VFH91_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET6862178.1"
                     /translation="MFTSRQSAAILLGLLVQFLAVTSQAQTTVDSRKIDWSTRVVEST
                     MKQYPGPKELGGWSYAKSLFLYGQYLVYRRTHDPRYLHYIKDWVDSHVDAQGVVTTTN
                     AQGVVSEVKFDNLDSMLPGNLLLILYQETGDQRYKIAADRIRKRFDSYPRTGDRGFWH
                     ATGKSREWQLWGDGVFMSMPFLIRYGKLFGDSKYADDEASSQLIIYGSHLNDPKTGLM
                     FHAYDESGQSVWADRETKHSAEIWCRAMGWFGMTLVETLDLLPRNHPSRPKLLAQLRR
                     LVAAWAKYQDPKSGLWFQVTNKPDDPNNWVETSSSSMFVYVIRQALANGYIPKAYRRI
                     EQTGYSGLLTKISLDENGDTRLVDICEGTNVGDLSYYLARKRLVNDFHGLGAFLIMNE
                     SFANPSFNNVKRSMWQPGH"
     gene            6904..7653
                     /locus_tag="VFH91_04005"
     CDS             6904..7653
                     /locus_tag="VFH91_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET6862179.1"
                     /translation="MTNSKTPKFTIVEPSRPGQTNEQVVARIYELIKELRPGDRLPPE
                     REFAKQLGISRPSLRAGLGSLISMGVLKSRQGAGTFLVDGPPALNSEPLRLLADLHGF
                     SYEHMFETRSILEVGATGLAAERATGDHLATMAEEITEMFVALNDPQQFLVHDMAFHR
                     AVAAASGNPTLATLVEMVSGILYERRRTTIERAHDFNEALEMHRKIYKAIRSRQPEAA
                     REAMREHIVRAQRAFALEEKQQRGRAEKDRA"
     gene            7842..>11294
                     /locus_tag="VFH91_04010"
     CDS             7842..>11294
                     /locus_tag="VFH91_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET6862180.1"
                     /translation="MSRQLSCRPVLLLLLITIMGVCSVSAQTITGSISGVITDSTGGV
                     IPGATVTLTGEKLGEKRTATTDEEGRYNFAALQPGRFVLKIERQGFQAYEQRDVILSA
                     NEKLALPDVKLQPGQVSETVSVTSEGAIVERESSDLTARLTADQLNLISTKGRDVTSL
                     LRLLPGTSNNDDIEGAGDGFGTDLPNVSGQRGRSTVPTIDGLFAGEPSGSNKLSFTIN
                     QDAVAEVKVLRNNYGAEYGNNGGAIINIVSKGGGKEYAGTAYYFLRNESLNAAPFFNN
                     KAGLPRPLYRHLYPGGNVGGPLPLPRFGEGGRFWLKDKAFFFFSYEKPHQITPNDPRF
                     VTVPTALERVGDFSQSIDSRNQKVFVRDPRNPGTGCSASDTSGCFKDPSRATASNLSG
                     LNIIPLSRLNSSGVALLNYFPLPNTFGGVGGAAFNYVFQSPTDVPKRSKVIRFDVTPT
                     TKDTIYWKYQWWTSDNLGTGTSGWPGNDNNRWGINSHYLYKDDGWSASWVRVVSASVV
                     NEFNFGMRHDSEGFIPGDGEIERLQRSALNYTAPQLFPQNNHLGTIPRATNWTGVRGP
                     TNGVANINWLDRWGEVGNDYIQPSFADNLSITHGDHALKFGVYYERLKNGEAPGGQWS
                     GVFNFRGDENTYTIDLGNTGFAYANAIVGNFRNYQETTARPFTNLRLTQFQWYAQDQW
                     KISRRFTLNYGVRFGWHSPFQQIDGQGSNFDPRLFDPNKVVALYENACAVSFTPPATC
                     PTNQRRAKDPTTGQLFLLVGPNAGLVGAIVPKTGNPNNGLALGNDPNTPPGYRITRSI
                     DVEPRLGMAWDMWGDGKTVLRLQGGIYHSPRVGGGTTGGNLVNNQPANRTFSIDYGNI
                     DILAALTGTAVTRPSSLNAVEVNSHTPTIYNLSIGIQRDIGFKTVLEASYVGSLARHL
                     GQRININDIPDGAKLGTNNISPVTGSRLADDFLRPYRGYGDITMVTWGGTSNYHSLQV
                     QANRRYTRGFQAGLAYTYSKSFDYANDDSSDVFFGRPFKDFNYGPSDFDQTHILTVNY
                     IYDVPSLSHKFDNGFVRALFDNWQISGTTSYASGKPKNLAVISSTGIVYSSTAATISK
                     LSPCPLGSNETSVAGETHVCTPITDFTGGGNNARPFVVCDPMKGDFGVDPTGTPLAFN
                     TACIV"
BASE COUNT         2722 a         2946 c         2809 g         2817 t
ORIGIN      
        1 taaaatggtt aggcgcagta tgaggcgctg aaccccaacc taaaggttgg ggagaatgag
       61 aggccgagtt tttacattcc gtcttcaacc atgaatagac gttatctagc tagaagtggc
      121 agcaccacga cgctaaaagt cggtggtgtc aaaaggtgtt agtgaacgag gggctaggtg
      181 gaaattgaag gaccacgggg ctgaagccgg gggtgttttg aaattgaaat caaacttaca
      241 gagttacgcc cagacattcg acgttgaatg tctgggcgtt gctactttga cactattaac
      301 tttacagcga gaacgttccg tagaaagcgc tgttctcacc tgagcgaccc cacggatcgc
      361 cgacgctctt accggaatag ttcgcgttca aggtatatcc aacttttctg ttctcaagcc
      421 cgaccagagg cttgtaccag acaagattcg gcatcttgca gcagagatcg tttggcatcg
      481 cctgaacgtt gattgttgct tcaccagcca ccacattgaa agacttatct tgatgatcga
      541 agcttaccgg tgtgtaccgc acggcgacga ctttacccaa tgccgccgcg tacttctcct
      601 cgagagcatg aaggaaagcc agagcctgcg ttcgtgatac gctgttatca atcaagatct
      661 cagattgtct ggcagcatca ctctcggcga ggttcgcgtc cgaactcaca accgctacag
      721 ctcgtacgcc cgtcagatca acgccttgcc attttccgga ggttacgctc caggccatca
      781 tagcgtcgcg accagtcgtg gtgacctcgc cgttgtagtg gcacgccccg gcgaagacgc
      841 tcgccgttcg aacctccacg taatcgcctc gtagactgac attctcagcg ctcgaactaa
      901 ataccaggca actgactgcc actattgcag ctaaacttaa aaacaataac cttctcatct
      961 tcgatacctc cgataacttt gcgcccttga ttatacgctc ttaattgcag aactgtagca
     1021 cgagcgtccg ggaccacact tatccggttc tacccgatta ttgtcggggc cgataacttc
     1081 ctgtgtttcg caacaccaaa cgtggttagc tgagtcgatg tagtcgtcag cactggtcgc
     1141 cagttggtct cgcatgaaaa actttttcga tctcaacgat ccacagtacg gcgattgaac
     1201 gggactcgag ttagcttcgt ttgccataag actctccaac gacatgggtt aaatggttga
     1261 tgcctttaaa ttcgtcgacg tttactctgg cgcctaaatt cctgctgttt tcgtcagcgc
     1321 tcgtctcacc agtgtttgcg taagtgggac cttgtacgca ttcttctcga gtggtttagc
     1381 ttcggataac gcaatcttac ctgcttcccc taaaacatca ggagtcaatg tttttccaat
     1441 caaaaacttc tctgccgcag gaacgcgcca ggggataggc gcgacacccc cgagcacaag
     1501 cctcacgtct ttaactttct ttcctgaatc aaattggacg gcagccgcga ctgaggccat
     1561 ggcgaaatcc aacgactcac gctctttaaa tttcagatag gtagactttt cagttagggg
     1621 cgaagccggc acaaacactt cggtgatgat atcgtcgttc ttcaacacgt tttcccgtct
     1681 gatgtttcct tcagagggga gagtgaagaa cttatcgagc gggattacgc gcttaccttc
     1741 cgcgccgact acggtgactc tggcgcctaa ggccaataac atcggagcca gatcggaagg
     1801 gtgcacgatg taggatgggc caccaccaat gattgcgttg tacttgttct ctccagttgc
     1861 tgcgtagcat tcggagccgc cttttttcaa acaaactact tcttcaagtc ggaaatacca
     1921 gcaacgtgga cgttggcata agtttccgcc gaccgttccg agattgcgaa tttgtggcgt
     1981 tgctattgaa tgagccgctt ccgcaagtcc gggcatcacc tttcgaatcc ctctgtgctc
     2041 ctctagttca tttagcgtta tcaaagcgcc aatcgtcagg ccctttgaat caagtgacag
     2101 ccgattcatg ccgcgaagat ttttcagatt gactaaacgt gccggtcgag aagtgtagtc
     2161 cttcatcgtt gtgagaaggt cctgaccgcc ggcgattggc cggggagctt catcgatgtc
     2221 agtcggggct gttgtcgaca ggagtttgac ggcctcattg atgtctttag ggttggtcca
     2281 ctcgaaggct ttcatctgtt ttcctctttg gagtgcggtg acctgtcacc gcttttgtta
     2341 tctgctaact cgaatccccg gcgcggacat taagcgccgc cgggccggcg cactccaaaa
     2401 gttatgtatt catctggccc attagaattt tgtcgggtgt tatgggtaac tcgcgcacgc
     2461 ggacgccaca ggcgttgaat actgcgttaa ccaccgctcc cagagctgga ataatgcaac
     2521 cttcggcaat gcctattacg gcttcgcgtt tatcctcgtc gtcgatgatc ggaacgagct
     2581 cgggcatttc aagggcgccc ggcaacttgt agtctccaaa accgggattc agttgcagac
     2641 caagctgagc atccatgact cggccttccc aaagtgccat acccagcgac tgaatcattc
     2701 caccattgat ctggctctcg agagtgagac ggttgagggg aagtccaccg tcctggacgt
     2761 ggaccagttt gatcggccga atatgtcccg tctcaacatc aacttccacc tcggcaaaag
     2821 aagcaccatg aattccgctg gcctgcagat cctgtcgcca ctcaccgcgt ccggtaattc
     2881 ctgccgcagg caatgctgca catgcttgtc tccacgccaa actcttaccc ttagcgctta
     2941 cctgaccgcc cgagaaagta atttcttcag gcttggcatt atcgagtagc ggcgccactt
     3001 tttctgagaa caatagcctc gctttgatgg ccccgtcttt aacggaagga gataaggaag
     3061 cagcggtagt tgagccacca gaagaattgg ccccacccag tttggaactg ccgatctctt
     3121 ctctgacatc attcacttgc agtccaaact cctcggcaac aatcgcccgg gtgaacgttc
     3181 gcgttcccgt cccgagatcc tgagtgccca cagcgaccag aactgagccg tcgcgcgaaa
     3241 tcgtgacttc gaccttacat tgattatttc caccgccgcc ccatgtgcca actgcgcaac
     3301 ccatcccgcg ctttactgga ccagcactag cgcctgccac tggatttcgt ttgtcccaac
     3361 caatttctct cgcgccgcga tcaagttgac ggtgataaac gacgtcgcgc aggttcttct
     3421 tccgaaacgc gactggatcc atcttgatct tgtacgccag ttcgtcaacc agcgactcga
     3481 ctgcaaaaga cgattgcgga tgacccggag cacgcattgc gacggaggag tcttcgttgg
     3541 tgtgaagcgc ataaacttca cgataggtgt cgcccatccg gtagatgtaa ggctgaccag
     3601 cttgtgagcc ttgtccaata ccgccaagtc gatactggcg cgcttgaaga gcgacgatgg
     3661 ttccgtcgtt ctttacacca gcttttagtt tttgccacga cgccgaccga ttgcctgcca
     3721 tcacaaactc gtcataacga gtaaacatca atttcaccgg agctttggcc tgttttgaca
     3781 atcggcaggc aaacattcct tcagtgccaa tgccgaactt cgatccaaat ccgccgccca
     3841 tgtgttggac gattgcggtt acagcgctct ggggcagtcc gagctccttt gcggcgtcct
     3901 cgggaatcgt aaatgttccc tgagtagacg catagatggt tgccgtatct ccgccgcgat
     3961 aatcgaccac catcccgtgt gtttcgagac aggcatgatg gattcgaggc gagatgtatt
     4021 cagcttcaac tatggcatcg gcggttgcga aagcggcttc tgctttagct ggatcgccgt
     4081 tcttattttt ctgctgaagg ttgctctcgt taggttcatc aacaactttc ggtgcatcag
     4141 gtttgatggc gtcggttgca tggacgacat gaggcagcac ttcgtattca actttaattg
     4201 cacgcaaagc atcggcggca acatcaggcg ttgttgcggc aactgcggca actggtgctc
     4261 cggcaaatct aacttcagtt aagggggcag ggataattgc ttttactcca ggtattttcg
     4321 acgcagcatc agtattcaaa ctcttgatcc gagcatgtgc gtgcggcgat ctcaggattc
     4381 gcgcgtacag cattccgggc aaccgctgat cgtaagtgta tttcgccgcg ccagtcgttt
     4441 tcagtggtcc atcaactcga gtcatccgat ggttgagcaa agtgtgtttg tcgtttggtc
     4501 cccaacccgg accgctgtct tcatcgactt cgatttcgac catcgtctcg atgccgttga
     4561 tcactctggg aactttgatt ttcttctttt tcttcgctgg agcttgttgc atggtccctc
     4621 cggagaacgc aattctctta aacagtttta ccactctttg cagccgctaa tgccgcttca
     4681 aaaactcgag gataagtgcc acaccggcag aagtttccct tgcaggcttc ccgaacttcc
     4741 gcaagactgg ggttaggatt tttctttaaa tatgcggaga ttgtcgtaac gaagcctggg
     4801 gtgcaaaaac cacacataag agcgtcgtgt tctacgaatg ccgcttgcac tgcattcggc
     4861 ttctcaggtg ttccgaggcc ctcaaccgta gttattttct tattagtggc atcgacagcg
     4921 agaacaaggc acgaatatgc aggcttccca tcaagtagca cggtacaagc cccgcaggtt
     4981 cctacatcgc agaccagttt aggaccggta atagcaggct cgagataatc tcgtatggta
     5041 ctcaagagtg ttgttcgcgg ttcaacctga acgctcctct tctgaccatt gatgtcgaga
     5101 gtgagactga cagtcccgct cagggtggtg gctggcgcga tcttttcctg agcttcgatt
     5161 tcaagagata acttgtctac cagagcagct ccggcacttg cgatgccgac tccttttaga
     5221 aacttgcgtc gacttggatc atgagccagc tctttatctt tatcgtcctc atccttggcc
     5281 atcttctact ctcctcgtac gctctcagtt ctgtcgtcag ttccttgaca ccatcacaat
     5341 cggtttcgta tgttggccga ccgaacttga aatggtaaga ccattttcgt ttcgtggtcg
     5401 ccgatctttg gaatcaaatt gtttgctgtc gacataaatt aacacaagcg attgtttacg
     5461 taataacaaa ttcttcaatc cagcgttatc cactaatacg ctacaccagg attactttcg
     5521 cgcgttcttc gacttcactt aagacacgga cgtatcacga atgtttactt cgagacaaag
     5581 cgccgcaata ttgttgggtt tactcgtcca gtttttagcg gtcacaagtc aagctcaaac
     5641 aacggttgac tctcgcaaga tcgattggtc tacgcgagtg gttgagtcca cgatgaagca
     5701 atacccggga cctaaggaac tgggcggatg gagttatgct aagtcgctgt tcctgtatgg
     5761 ccagtacctc gtatacaggc gaacccacga cccacgctat ctgcactaca tcaaagactg
     5821 ggttgattcg cacgtggatg cacaaggcgt cgtcacgact accaacgcgc aaggagtcgt
     5881 gtcagaagta aagttcgata atttagacag catgctgccg ggaaatctcc tgctcattct
     5941 ttatcaggaa acaggagacc agcggtataa aattgcggcc gatcgaataa gaaaacggtt
     6001 cgactcttat cctcgcaccg gggatagagg cttctggcat gcaaccggaa agagccgcga
     6061 atggcagctc tggggagacg gggttttcat gtccatgccc ttccttattc ggtatggaaa
     6121 gctctttgga gacagcaaat acgctgatga cgaggcgagc agtcaactga taatttatgg
     6181 ttctcactta aacgatccta agaccggact aatgtttcac gcctatgacg agagcggtca
     6241 atcggtttgg gccgatcgag agactaagca ttcggctgag atttggtgtc gagccatggg
     6301 gtggttcggc atgacattgg ttgagacttt ggatctgctg ccgcgaaatc atccttcccg
     6361 accgaaactc ctcgctcaac tgcgacgcct ggttgcggcc tgggctaaat accaggatcc
     6421 aaaaagcgga ttatggtttc aagtgacgaa caaaccagat gacccaaata attgggtgga
     6481 gacatcgagc tcaagcatgt ttgtctacgt gattcggcag gcgctcgcta acggttacat
     6541 tccgaaggcg tatcgtcgaa tagagcaaac gggttactca ggactcctca cgaagatttc
     6601 gctcgacgaa aatggtgata cgcgtctggt cgatatctgc gaaggcacaa acgtgggtga
     6661 cctttcctat tacctcgcgc gcaaacgatt ggtaaacgat tttcacggcc ttggagcgtt
     6721 tctaattatg aatgagagct ttgccaatcc ttcattcaat aatgtgaagc ggtccatgtg
     6781 gcagcccggc cattgagttc aaaccttatc tagccgcctc tgtttcttct aaaattttct
     6841 ctgaaaagtc ttgacggctt agaaaattgg tattataagt cagacgctgg ccagtaaatt
     6901 gttatgacca attctaaaac tcctaagttt actatcgtcg agcccagcag gcctgggcag
     6961 acaaatgagc aggtcgttgc ccgaatttac gagttgataa aggagctgag acccggtgat
     7021 cgcttgcctc ctgagcgcga attcgcaaaa cagctgggaa taagccgccc atcattgcga
     7081 gcaggattgg gatctttgat ctcaatggga gtactcaagt cgcgacaggg cgccgggact
     7141 tttctggttg atgggccgcc agcgcttaac agcgagcccc tcaggttgct ggccgatctg
     7201 cacggcttca gttacgagca catgtttgag acgcgctcga ttctcgaagt aggagcaacc
     7261 ggccttgctg ctgaacgcgc gactggtgat cacctcgcga caatggcaga agagatcaca
     7321 gagatgtttg tagccctaaa tgatccacaa caattcctgg ttcacgacat ggctttccat
     7381 cgagccgtcg ctgcagcatc aggaaatccg acgctcgcga cgttggttga gatggtttcc
     7441 ggcattctct acgagcgcag gcggacaacc atcgagcggg ctcatgattt taatgaagcg
     7501 ctcgagatgc atcgaaaaat ctacaaggca attcgctcgc ggcaaccaga agctgcacga
     7561 gaagcaatgc gagaacacat cgtccgggcg caacgggcat ttgctctcga agagaagcag
     7621 cagagagggc gagctgaaaa agaccgagct taattttttc caaatgttta cactgggcga
     7681 atcacctcaa tacgtaaccg ataggtcgat caaataaagc aattttcctc ttaagttttt
     7741 aagcgcgcag tttgttcaaa ttgtccgaac aaaagacaaa ttctggttga gccaagtgaa
     7801 ggtcaacctt cgaacctctg aacaatttaa ggaggagtcc catgtctcgt cagttatcat
     7861 gccgacccgt tctgctactg ctgctcatta caataatggg tgtctgttcc gtaagcgctc
     7921 aaactataac cggatctatc tctggtgtta ttactgattc cactggaggc gttattcccg
     7981 gcgccaccgt tacgctcaca ggggaaaagc ttggtgaaaa aagaactgca actactgacg
     8041 aggaaggtcg atacaacttc gcggcattgc agcccggcag gttcgttctg aagattgagc
     8101 gacaggggtt ccaagcctac gaacaacgag atgtgatcct aagcgcgaat gaaaaactcg
     8161 cgttgcccga tgtcaagctt cagcccggcc aggtatcgga aaccgtgagt gtcaccagcg
     8221 aaggagcgat cgtcgagcgt gagagtagtg atttaactgc acgattgacc gccgatcagc
     8281 tcaacttgat atcgacgaaa gggcgcgatg tcacctcact cttacggcta ctccctggta
     8341 cgtcaaacaa cgatgacatc gaaggtgctg gtgatggctt cggaactgac ttgcctaacg
     8401 tgtcggggca gcgcggccga tctacggttc caacgatcga cggattattt gcaggtgagc
     8461 caagtggctc aaacaaactc agcttcacga taaaccagga cgcagttgcg gaagtcaaag
     8521 tgctccggaa caactacggc gctgaatacg gcaacaacgg tggtgcgata ataaatatcg
     8581 tttcgaaggg cggaggaaag gaatacgcag gtacggccta ttatttcctg cgcaacgaat
     8641 cgcttaatgc tgctccattc ttcaataata aagctggtct accgagacca ctttatcgac
     8701 acctctatcc cggcggcaat gttggcggcc cgctgccgtt accacgattt ggcgaaggcg
     8761 gaaggttttg gttgaaggat aaagctttct tcttcttctc ttacgagaaa ccacaccaga
     8821 ttacgcctaa cgatcctcgt tttgttactg tgccaacagc attagagcga gttggcgact
     8881 tctcgcagtc gatcgactcc cgcaaccaaa aagtatttgt tcgcgatccc aggaacccgg
     8941 gtacgggctg tagtgcaagt gacacgagcg gctgtttcaa agacccgagt cgggcaacgg
     9001 catcaaatct cagtgggctc aacattattc ctctgtcccg attaaattcg agcggcgtgg
     9061 ctttgctcaa ttattttccg cttccgaata cgtttggtgg cgtaggagga gcggcattca
     9121 actatgtgtt tcaaagtccg accgacgttc cgaaacgcag caaggtaatt cgtttcgatg
     9181 tcacacctac taccaaggac accatctatt ggaaatatca gtggtggacg tctgacaatc
     9241 ttggcacagg gacgtctgga tggcccggaa atgacaataa ccgctggggc atcaattcgc
     9301 attacctgta caaagatgat ggctggtcag cgagctgggt tcgagtcgtc agtgccagcg
     9361 tagtaaacga gttcaatttc ggcatgcgtc acgactcgga aggtttcatt ccaggtgatg
     9421 gcgagattga acgtctgcaa aggagtgctc tgaattacac agctcctcaa ctgtttcccc
     9481 agaacaatca cctgggaacg attccaagag ccacaaattg gaccggtgta cgtgggccca
     9541 cgaacggcgt tgcaaatatc aactggttgg atcgttgggg tgaggttggc aatgattaca
     9601 ttcaaccctc attcgccgat aatctttcta taacgcatgg agaccatgca ttaaagtttg
     9661 gcgtgtacta cgagcgcctg aaaaacggtg aagcgccagg gggccagtgg tcaggtgtat
     9721 tcaattttcg tggcgacgaa aacacttaca ccatcgatct tgggaacact ggattcgcct
     9781 atgcaaacgc tatcgtcggc aatttccgaa actatcaaga aacaacggct cggccattta
     9841 caaaccttcg gctgacgcag ttccagtggt acgcgcaaga tcaatggaag atcagccgca
     9901 ggttcactct taactacggc gtgcgctttg gctggcactc tccattccaa cagatcgacg
     9961 gtcaaggttc caactttgat ccaagacttt ttgatccgaa taaagtcgta gctctttacg
    10021 agaatgcttg cgcggtttcg tttactccac ctgctacctg cccaactaac cagcgccgag
    10081 ctaaagatcc aacgacggga cagctatttc tgctggtggg tccaaatgcc ggtttggtcg
    10141 gggcaattgt tccaaaaacg ggcaacccga ataatggtct cgcgctcggt aatgatccga
    10201 atactcctcc gggataccga atcaccaggt ccatcgacgt tgaaccacga ttgggaatgg
    10261 cttgggatat gtggggcgac ggtaaaacgg ttctacggct gcagggaggt atctaccact
    10321 cgccgcgagt cggtggtggt actacaggtg gcaatctggt caataaccaa ccagctaatc
    10381 gcacctttag tatcgactac ggcaacattg atattctcgc tgctttgaca ggaaccgctg
    10441 tcacacgacc ctcttccctt aatgcagttg aagtcaactc acacacacca acgatctaca
    10501 acctctcgat tggtattcag cgcgatatcg gtttcaagac tgttttggag gcgagttacg
    10561 ttggatcgtt ggctcgacac ctgggccaga gaatcaacat caatgacatt ccggacggcg
    10621 ctaaacttgg aaccaacaac attagcccag taacaggaag tcgactcgct gacgatttct
    10681 tgagaccgta tcggggttat ggcgatatca ctatggttac gtggggtggc acatcaaact
    10741 accactcact gcaggtccaa gcgaaccgac ggtacacgcg aggctttcaa gctggtctcg
    10801 cctacaccta ttcgaaatcc tttgactacg cgaacgatga ctcatcagat gtcttcttcg
    10861 gtcgcccgtt taaggacttc aattatggac cgtctgactt tgaccagact catatcctta
    10921 cggtcaatta catttacgac gttcccagcc tgagtcataa gttcgacaac ggcttcgtac
    10981 gagcactttt cgacaattgg cagatttcgg gaactacttc ttacgccagc ggcaagccaa
    11041 agaacctggc ggtcatcagt tctacaggta ttgtttatag cagtactgct gcgacaatta
    11101 gtaagctgtc accttgtccc ctcggttcca atgaaacatc tgtggctggg gaaacacacg
    11161 tatgcacgcc gatcacagat tttactggtg gtggaaataa cgctcgacca tttgttgtct
    11221 gtgatccaat gaagggcgat tttggagttg accctactgg aacaccgcta gcattcaaca
    11281 cagcttgcat tgtg
//
LOCUS       DASSFP010000185         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1684415, whole genome shotgun sequence.
ACCESSION   DASSFP010000185 DASSFP010000000
VERSION     DASSFP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684415"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..616)
                     /locus_tag="VFH91_04015"
     CDS             complement(86..616)
                     /locus_tag="VFH91_04015"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET6862181.1"
                     /translation="MKALIVSICLMSIVLVGCSHLKSLHQTNASSEPSPEANASPALQ
                     PSGRGTPPEAKAMMLKAVEHYNAVGRTKALADFTAMKSPFGDRDLYVVCIGSNHKITA
                     NGGFAQYVGVSSDVWKDADGKSLGQAIWNAAWEPGEGTVQYKWINPVSHVTEKKIGFF
                     KKVGEDVCGVGAYQPE"
     gene            complement(667..816)
                     /locus_tag="VFH91_04020"
     CDS             complement(667..816)
                     /locus_tag="VFH91_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862182.1"
                     /translation="MKRNGMVGYPIIAMGIAFLTIGFNGRKAFIAIGLAFMLIGIVML
                     RRRVR"
     gene            complement(832..1647)
                     /locus_tag="VFH91_04025"
     CDS             complement(832..1647)
                     /locus_tag="VFH91_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862183.1"
                     /translation="MSTPLPSSSPSPAVETFSLVKQFGGFVAVDHIDLQVRRGSFFGF
                     LGPNGAGKSTTIKMLTGLLPPTGGKLRVLGLDITEQPMAVKRSIGVVPEDLNLFERLT
                     GAEMLSFTGRMYGLSKSDIAQRSKELLELMELQEEPRKLIIEYSHGMKKKLSLACALI
                     HRPEILFLDEPFEGVDAIASRTLKDLLSRLTARGLTVFLTSHVLEIVERLCSDIAIIA
                     QGKLLASGALDELRKGIRLEGDGAGPVSLEEYFIHVVGGTRSTGEADVLQWLT"
     gene            1802..3322
                     /locus_tag="VFH91_04030"
     CDS             1802..3322
                     /locus_tag="VFH91_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862184.1"
                     /translation="MKRFLERHRDRITGTLSGFDRMLFRGTLRSITHCKGLEIFLHSQ
                     YVLMKDFGAYVIRLSEGLVAHAKQVASEAGRPYQYLPSAKESKEELALQIAKRDRIKQ
                     GLICVFACVEPCQSFDLRKDRASGHLKLVTKERKCQHLYFYYLDREFGLMHVRLQSWL
                     PFSIQVCINGREWLARQMDRAGISYQQRDNCFSYIADPQRAQQLMDRLTARNFNPWLD
                     KLARKVNHWMDPQSGLDIRSYYWSGRQMEYATDVMFRSSDSLAELYPLLLRHAMFQFG
                     SEEVMRFLQRHTNKRFAGEVSSDLQRRVEGIRVKHRVEENSIKMYDKQGSVLRIETTI
                     NNPRRFKVRRKGWRRGKPVMKWLPLRKGIADLRRRAELSQGANARYLDALAVVGLQTP
                     SYQLLDGVSAPVKAKRQYRALRPISSADASLFQVILHGKFALQGFRNRDLRQALGQPA
                     VKNISARISRLLALLRAHKLIFKVCKTNYYRITRKGHQVMATALKFRQPDLALLAA"
     gene            complement(3430..>3878)
                     /locus_tag="VFH91_04035"
     CDS             complement(3430..>3878)
                     /locus_tag="VFH91_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862185.1"
                     /translation="IFFVPDMTSAVSELWRLVRPGGKLALTTWGPNFFEPASTVFWNS
                     IREVRPDLYKGFNPWDRICDPASLNDLLRSGGAELVETHAESGTHEVASPEAWWAAVL
                     GSGYRGTVDQLDETDREHVCKVNLNYIRESGINSVEANVVYGVARK"
BASE COUNT          957 a         1000 c          965 g          956 t
ORIGIN      
        1 gatcatttca gatttaagat tgcagatttc agattagaac acttcgaacc atcaatctga
       61 aatctgcaat cttaaatctg aaatactatt cgggttgata cgctcccacc ccacaaacat
      121 cttcaccaac ctttttaaag aacccgatct ttttctcagt cacatgcgag acggggttaa
      181 tccacttgta ctggaccgtt ccctcacccg gctcccatgc cgcattccag atggcctgac
      241 cgagtgattt accatcggca tccttccaaa cgtcggatga gactccgaca tactgagcga
      301 atccgccatt cgcggtgatc ttgtggtttg acccgataca aactacatac aggtcgcggt
      361 cgccaaaagg tgacttcata gcagtgaaat cagcaagcgc tttggttctg ccaaccgcgt
      421 tgtaatgctc gaccgctttc aacatcattg ctttcgcttc tggaggcgtg ccacgtccac
      481 tcggttgtag agccggtgat gcgtttgctt caggactggg ctccgacgag gcgttggttt
      541 ggtgaagaga tttgaggtga ctacagccaa ccaggacaat ggacatcaga caaattgaaa
      601 ctattagtgc tttcatagat gcctccttgg tcctcagatc ctcaaattta aataaagact
      661 acaatttcat ctcaccctgc gacggagcat tacgatacca atcaacatga aagctagtcc
      721 gatagcaatg aaagctttgc gtccattgaa gccaatcgtc aggaacgcga tgcccattgc
      781 aatgattggg tagccaacca ttccgtttct tttcatttat ccctccgctg atcaggtcaa
      841 ccattgcagc acgtctgctt cacccgtact gcgcgtgccg ccgacgacgt gaataaaata
      901 ctcctcaagt gacactggcc ctgcaccgtc gccctcgaga cgaattcctt tgcgcagctc
      961 gtcaagtgca ccagatgcca agagttttcc ctgtgcaatt atggctatgt cactacacaa
     1021 gcgctcgact atctcgagga cgtgtgacgt caaaaacacg gtcaatccac gagcagtcag
     1081 ccgagataac aggtctttca gagttctcga agcaatcgcg tcaactcctt cgaaaggttc
     1141 atcgagaaac agaatctctg ggcggtgaat caatgcacaa gccagtgaca gtttcttttt
     1201 cataccgtgt gagtattcga tgatcaactt cctgggctct tcctgcaatt ccatcaactc
     1261 gagtagctct ttcgagcgct gggcgatatc actcttgctg agtccataca ttcgtccggt
     1321 gaacgagagc atctcggcac ccgtcagacg ctcgaacaaa tttaggtctt cagggactac
     1381 accgatgctt ctcttcactg ccatcggttg ttcagttatg tccagaccaa gcacgcgaag
     1441 cttgccgcca gttggcggca acaagcccgt cagcattttt atcgtcgtcg acttgccagc
     1501 tccattggga cccaggaaac cgaaaaaact tccacgacgc acttggaggt cgatatgatc
     1561 gactgcgaca aacccaccaa actgctttac aagagaaaat gtctcaactg ccggagatgg
     1621 cgaggacgac ggaagcggag ttgacatgca cgcctttgta cgcggtgagt gagcgttagg
     1681 tcaagcagag tatccacaga ttacacagcg cggcttaggc cgcaaccaaa ataggaacgc
     1741 ttcatgaccg ccttctggca tgatctgttt gaaccaacaa acggacggcc ggaggcgatt
     1801 catgaagcgt tttctggagc ggcaccgcga ccgcataacc ggaactcttt cgggctttga
     1861 tcgaatgctg tttcgcggca ccctgcgttc gataactcat tgtaaggggt tggagatctt
     1921 tctgcatagt cagtacgtgt tgatgaaaga ctttggcgct tacgttattc gtttgtcgga
     1981 gggcttggtt gcgcatgcga aacaagtggc gagcgaggcc ggacggcctt atcagtactt
     2041 gccctccgcg aaagaatcga aagaggaatt ggcgctgcag attgcgaaac gcgaccgtat
     2101 taaacaagga ctgatctgtg tgtttgcctg tgtcgaacct tgtcaatcgt ttgatctgcg
     2161 aaaagaccga gctagcggac atttgaaact ggttacgaag gaacgtaaat gccagcatct
     2221 gtacttttat tatttggacc gcgagtttgg cctgatgcac gtgcgcttgc agagctggct
     2281 gccgttcagc attcaggttt gtatcaatgg acgcgaatgg ttggcgcggc aaatggatcg
     2341 tgctggcatt agttaccagc aacgagataa ctgtttttcg tatatcgctg acccgcagcg
     2401 cgcacaacag ttgatggatc gtctgacagc tcgcaacttc aatccctggc tggataagtt
     2461 ggcgcggaaa gtgaaccact ggatggatcc ccagagcgga ctggatatca gaagttatta
     2521 ctggagtggc cggcaaatgg aatacgctac cgatgtgatg tttcgcagca gcgacagtct
     2581 ggcggaactt tatccgttgc tgctgcgtca tgccatgttt cagtttggta gcgaggaagt
     2641 aatgcgcttt ctgcagcgcc acactaacaa gcggtttgcg ggcgaggtca gcagtgacct
     2701 gcaacggcgg gtggaaggca tccgcgtgaa acatcgggtt gaagagaact cgatcaaaat
     2761 gtacgacaag caaggcagcg tgctgaggat tgagacgacc attaacaacc ccagacgttt
     2821 caaagtacgg agaaaaggct ggcgccgggg aaagccagtg atgaaatggc tgccgctgcg
     2881 caaaggtatt gccgatctgc ggcggcgtgc tgagttgagt cagggcgcta acgctcgcta
     2941 tttggatgct ttggccgtcg tcggtttgca gactccttct taccaactgt tggacggagt
     3001 cagcgcccca gtcaaagcta aacggcagta tcgtgccttg cgcccgatta gttctgcgga
     3061 cgcgagcttg tttcaagtca ttctgcatgg aaaatttgcc ttacaaggct ttcgtaatcg
     3121 tgatctgcgt caagccctgg ggcaaccagc tgtgaaaaat atttctgcgc gcatcagtcg
     3181 cttgttagca ctcttacgcg cacacaaact gatctttaag gtctgtaaaa ctaattacta
     3241 ccgcattact cgcaaaggcc atcaagtaat ggcgaccgct ttgaagtttc gacaacctga
     3301 tcttgctctt ttagcagctt agtctcttgc taaaaaaaca agaagttgtc ggtttgttac
     3361 acagattaca ccgattttaa gcacgcatgc tgaagaacca ctcgaagaaa tctgtgtaat
     3421 ctgtggatac tatttccgcg ccacgccgta cactacattc gcttcaaccg aattaattcc
     3481 cgactcgcga atgtagttca ggtttacttt gcacacatgt tcgcgatcgg tttcgtcgag
     3541 ttggtctacg gtgccgcgat aacccgaacc aagtacggca gcccaccacg cttcaggtga
     3601 tgccacttcg tgcgttccgc tctcggcatg tgtttccacc aactctgctc ctccactacg
     3661 aagcagatcg tttaacgagg ccggatcgca gattcgatcc catggattga agcccttgta
     3721 gaggtcgggt ctgacttcgc gaattgaatt ccagaacacg gtgcttgctg gctcgaagaa
     3781 gtttggtccc cacgtcgtga gtgcaagctt gcctccaggt cgaaccagtc tccagagttc
     3841 actgactgca gacgtcatat cgggaacaaa gaaaatac
//
LOCUS       DASSFP010000186         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_346082, whole genome shotgun sequence.
ACCESSION   DASSFP010000186 DASSFP010000000
VERSION     DASSFP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346082"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFH91_04040"
     CDS             <1..1146
                     /locus_tag="VFH91_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862186.1"
                     /translation="ANRATIDIASNDFAPDNRFYFTLRREVPARALIIESATRGGSDS
                     VHLQSALTTNDDLPFSFTVKTTGSVDPGSIAENSLLILNDSGPLPNAVADSIVRFVKA
                     GGQLIVSTGPHTDATAFNRSLESIAPAELKEAVQTKPGESTSITSVKFDHPIFEVFQQ
                     SGRLASAQVIGYYRSEPHNNITVLARFEDGSPALLESLPDKGRVLMFTTSLGPSWNDL
                     PLTPIYLPFIHQMVRYAGSRDEDAWYGLGQTFTVRKQVEGIPAVDSPGGNRLSETKLT
                     ANGDVLITANEPGFYRLRYASRPDFAAVDTDGMEGDFRKLDFGQFVAAVTGGAGNAEG
                     ANVNSTATSEETEGRQRVWWSLLLIALLLLVTESIVARRTKVAKMVG"
     gene            1265..>3585
                     /locus_tag="VFH91_04045"
     CDS             1265..>3585
                     /locus_tag="VFH91_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HET6862187.1"
                     /translation="MNPEHKTLQSLLQKTRSRRKLLLTLRGFAIVLAVLAVVLLLTGW
                     TAHRYRYNSNALVALRTGAFLSVLATIYFALLRPLLKRISDFRLARLIEEHSPGTEDR
                     LVTAVEYGKEDPGRQISPAILNRLYRDADDSSATVELGNVIRRSRLVFYGASALASLL
                     IFGAVLKWGPKEISQGVTQLVSPTTLAASTNAMSIKVRPGTAKVPKGSDQDVIASLVN
                     FDSETVTLFSRPLGSKNDFQGQPMEPAKARSDYRFLIFYIQDSMEYFIESNNVRSDVF
                     KLDVVDLPFVKQLDLTLNFPSFTHLPPKVLEDGGDIAAIKGTAAMVTAKLSGRVKGAR
                     LVFADGRKIEMKPEGADFRGEVSVVADSTYYIELVSVDGEAYRGSNEYDITLLTDQPP
                     TISFDKPGRDKKATNLEEVFTQARAEDDFGVVSMDLHFSINGGEEKKVNLQELTRESA
                     QSLTGAYTFFLEDYKLKPGDFISYYAKARDANNEATSDIYFIEVKPFEMQYHQSQQQG
                     GQGGGGDQDQNALSRRQKDLIAATHRLIRESDKYTEQERKDSYEVVASGQEKLRTDTL
                     EFLDRMGRRLGGDLDSQKQVAEMAEHLRQAAKEMEGAPPPLRKETGKDALPPEQRALQ
                     RLLAADAIFREVQVAFGNQSGGGEGGAEQQQQELAGLFELELDKMKNQYETVQRAQQQ
                     QAQQQKSEAERRLEELARRQQQALEEQRRRMGQPANGSSGGSQRQQQQLIEETRKAAR
                     ELERLSREKRDTRMQELSRQLNQTADEMQKAQAS"
BASE COUNT          958 a          881 c          955 g          791 t
ORIGIN      
        1 gccaatcgag ccacgatcga cattgcctct aatgattttg ctcccgacaa tcgattctat
       61 ttcacgttgc ggcgagaagt accagccagg gcgttgatta tagagagtgc aactcgcggt
      121 ggcagtgaca gtgttcacct gcagagtgca ctgacgacga atgatgatct accttttagc
      181 tttacagtaa agacgactgg atctgttgat ccgggctcta tcgcagaaaa ctccttgctc
      241 atcctgaacg attccggtcc acttccgaat gcggtcgccg acagtatcgt tcgatttgtt
      301 aaagcaggcg gtcaattaat tgtctctact ggaccacata ccgacgctac cgctttcaat
      361 agatctcttg agtcgattgc ccccgcggaa ttgaaagaag cggtacaaac caaacccggc
      421 gagagtacgt ccatcaccag cgtcaaattc gaccatccaa ttttcgaggt attccagcaa
      481 agtggccgcc tggcttcagc acaagttatc ggctattacc ggtccgaacc tcataacaac
      541 atcacagtgc tggcgagatt tgaagacggc agtcctgcac tactcgagtc actgcctgac
      601 aaaggtaggg tgctgatgtt tacaacatct cttggtccat catggaacga tctgcctctg
      661 acaccaatct atcttccgtt tattcatcag atggtcagat atgccggatc gcgtgatgaa
      721 gatgcctggt acggactggg ccaaactttc accgttcgca agcaggttga agggatacca
      781 gccgtcgatt caccgggagg aaaccggctt agtgagacta agcttacagc gaatggagac
      841 gtgctgatca cggctaatga gcctgggttc tatcgccttc gctatgcgag tcgaccggac
      901 tttgcggcgg tggatacgga tggaatggaa ggagatttcc gcaagttgga ttttggacag
      961 ttcgtggcgg cagtaactgg cggcgctgga aacgctgaag gagctaacgt caacagcaca
     1021 gctaccagtg aagaaacaga aggccgccag cgggtttggt ggtcgttgtt attgatagca
     1081 cttttgttgc ttgtgactga atcaattgtt gcgcgaagaa cgaaagtcgc aaagatggtt
     1141 ggctgaaacg gtctgtgtag cagtatgctg ttactccggc cgcgagtcgt ctccccacaa
     1201 aatgaaagta gactgacctt cgtatgtgag gtcgttttca attttttgga cagggccaga
     1261 aatgatgaac cctgaacaca agacgctaca aagcctgctg cagaagactc gctctcggcg
     1321 aaagttattg ttgactctcc gaggattcgc aatagtactc gccgtgttag ctgtagtgtt
     1381 gttgttaacc ggatggaccg ctcaccgcta ccgctataac agtaacgcac tcgtcgccct
     1441 ccgcaccggg gccttcctga gtgtcctggc aacaatttac tttgcactgc tgcgaccact
     1501 gttaaaaagg atcagcgact ttcgactagc gcgtctaatt gaagaacact cccccggcac
     1561 agaagacaga ctggttaccg cagttgaata tgggaaagaa gacccagggc gccagatctc
     1621 acctgcaatc ctgaatcgac tctatcgcga tgccgacgac tcatcagcaa cggtggagtt
     1681 gggcaatgta atccggcgct cacgtctggt tttctatgga gcgtcggcac ttgcgagcct
     1741 gctcattttt ggcgctgtgc tgaagtgggg tccaaaagag atttcgcaag gcgtaacgca
     1801 actggttagt ccaactacgc tggctgcctc gacaaatgcg atgagcatca aggttcgacc
     1861 aggtactgcg aaagtcccca aaggttcgga tcaagatgta atcgcctcgc ttgtaaactt
     1921 tgactcggaa acagtcacgc tcttttccag accacttggg tccaagaatg actttcaggg
     1981 ccagccaatg gaacccgcca aagcccggag cgactatcgg ttcttgattt tttacattca
     2041 ggactcgatg gaatatttca ttgagtcaaa taatgttcgt tctgatgttt tcaaactgga
     2101 tgtcgtcgac ttgcccttcg tcaaacagct cgatctgact ctcaactttc cctcgttcac
     2161 acacttgcct ccaaaggtac ttgaagatgg tggggacatt gcggcaatca aaggtacggc
     2221 tgcgatggtg acggcaaaac tctcgggaag agttaaaggg gctcgacttg tctttgcgga
     2281 cggccgaaag atcgaaatga aacctgaagg cgcagacttc agaggagaag tctccgttgt
     2341 tgctgattca acttattaca tcgaacttgt aagcgtcgat ggtgaagcct accgcggttc
     2401 taatgaatac gacattacgc tgctcacgga ccaaccaccc acgataagtt tcgacaaacc
     2461 aggacgggat aagaaagcca ctaaccttga agaagtcttc acgcaagcgc gcgctgaaga
     2521 tgactttggc gtcgtttcga tggacctgca tttttcgatc aacggcggtg aggaaaagaa
     2581 agttaatctt caggagctaa ctcgcgagtc ggcgcagtcg ctgacaggtg catacacttt
     2641 ctttctcgag gattacaaac ttaagccagg agattttatc tcttattacg ccaaagcccg
     2701 tgatgcgaat aacgaggcca ccagcgatat ttacttcatt gaggtgaagc cctttgagat
     2761 gcaatatcac cagtcacaac agcaaggtgg ccagggcggc ggtggtgatc aggaccagaa
     2821 tgctctgtcg cgccggcaaa aagatctaat cgcggcgact catcggctga tcagggaaag
     2881 cgataagtac accgaacaag agcggaaaga cagctacgag gtagtcgcat ccggccagga
     2941 gaaactacgg acagatacgc ttgaatttct tgatcggatg ggacgccggc tgggtggaga
     3001 tctcgatagc cagaagcaag tcgccgagat ggcagaacat cttcgtcaag cagctaaaga
     3061 gatggaaggt gcacctccgc cattacgaaa ggagactgga aaagacgctc taccacctga
     3121 acagagagct ttacagagac tactggctgc cgatgcaatt ttccgggagg tccaggtagc
     3181 gttcggtaat caaagtggcg gtggagaagg aggcgcagaa cagcagcagc aggaactcgc
     3241 cggtctattc gagcttgaac tcgacaagat gaagaaccaa tacgaaacgg ttcagcgtgc
     3301 gcaacaacaa caagcacaac aacagaagag cgaagcggaa cgaaggctcg aagaattggc
     3361 gcggcggcag caacaggcgc tggaagagca gcgacgaaga atgggccaac cggcaaatgg
     3421 atcgagcggt ggcagtcaac gtcaacaaca gcaattgatt gaagagactc gtaaagcagc
     3481 tcgagagttg gaacgcctgt cgcgcgaaaa acgtgatacc cggatgcagg agttaagccg
     3541 gcaactgaat caaactgccg atgaaatgca aaaggctcag gcctc
//
LOCUS       DASSFP010000187         6284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1821434, whole genome shotgun sequence.
ACCESSION   DASSFP010000187 DASSFP010000000
VERSION     DASSFP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821434"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFH91_04050"
     CDS             <1..673
                     /locus_tag="VFH91_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6862188.1"
                     /translation="QGAEEADGIDSLTRVVRDQIGHGADWIKVYADYRWGTQGAAPTF
                     SLEELKLAVETAKSANVPVAAHATTTEGMRRATLAGVETIEHGDGGTPEVFQLMKERG
                     VALCPTLAVAGANAERKKAVFQAALNSGVTIASGSDVGVFAHGDNVRELEAMVNFGMS
                     ALAALKSATSVDARVLHMAEKIGSVKSGLLADLIAVEGDPTQDISTLRKVRLVMKGGT
                     IYKTP"
     gene            complement(690..1064)
                     /locus_tag="VFH91_04055"
     CDS             complement(690..1064)
                     /locus_tag="VFH91_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6862189.1"
                     /translation="MSATILIAEDYDDNRELLRLLLSAANYKVREARNGQECLNLARQ
                     NPPDLIMVDLSMPELDGWEVFRELKADSSTADIPCVAVTARTDLDRGRALQVGFSAFV
                     SKPFKTEELLATVARLVASQTV"
     gene            1531..2931
                     /locus_tag="VFH91_04060"
     CDS             1531..2931
                     /locus_tag="VFH91_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862190.1"
                     /translation="MVNGQLENSLRHEIETYLESRLSAMKQDISALQSLLNESLSGLV
                     ERQSDVPMEGSLSTAISEHLRAAHERGIELAASESSRAKASSDMAIVKAGITEINEQG
                     NQSEILKALINRAASFAPRVAFFVIKGDRASGWRGRGFEGTVGDNSIQEISVSLSSDT
                     VLGSAAKSLTSWSGGPGSHSEDHLVLNRLGEEPSQRIVAIPLIVRGRAVAVLYADSAG
                     LDSESINLEALETLVKVTGMAVELLSARSAPKRASEPAAAKEPAPSYSPTSDYQESTP
                     AASSFDFSSLKEAEPAQSSYEELPPAEMSVVEEAPAAGYDIAPVSESFTETPSWRDES
                     PASSSFEPEESKPIAPPPSQTKRRYGHETELPVEVSSDEERRLHNDALRFARLLISEI
                     KLYNEQKVVEGRSEHDLYDRLREYIDRSREMYDKRVKPEVASRYDYFHGELVNTLAEG
                     DVSKLGSNYPGATVNA"
     gene            3049..5487
                     /locus_tag="VFH91_04065"
     CDS             3049..5487
                     /locus_tag="VFH91_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6862191.1"
                     /translation="MRPGLRTGVTFHRVVIESLSFSKFVQSGDDSPRLKIVGKHRLVI
                     VVLGVSVLAVASLSFLLSASCLTRPQTLGEQKALEQMRSMTRNDVLPSEDAVLRIENQ
                     FKGTKAAALARLLRARVKLNAKDFAGAADLLQSKTINEQTALGDYALYLRGTALEDGN
                     RQVEARATYQQLLQSFPTSLRARDAALRAANLLSKSGESAAIPLLLRDFNQADEPAAL
                     LATAKAYEQTSDQTRALATYRRLYFFAPSSTESAEAATAIPRLGSTTSPATVDEAITR
                     AGLLYDARRFVDAINAYGEAFARFPATVTTENQLRRGIAASNARKVPEAVAALSSIPT
                     SAGEARAEGLFYLAQTYARARQWEQARSTVEELRRSFPSSNFTPRAFVSVGQIAEDAK
                     NPTDASYFFKTAVNSFSNSIEVAQAQFEIAWAAHESRNFADSSKMLTEHLAFYADRNT
                     DNRGRAGYWAARDSERAGKLAEARALYEAMQARYDANWYGYLAKQRLDVISRSAPAKN
                     FPDDSIVARAVANLKTVTVAEETAGPEEEARILKADQLSNVGLDDWAIKELDKAGEKH
                     QDSPKINLAEARIYRSDDDNVRALNTLRRSFPDYSQMKPEEMTREEWDVFYPLAYWDI
                     IVQESRNRNLDPYQVAGLIRQETIFSARARSSANAYGLMQVLVPTGKLTARKYGMGRE
                     VTADSILEPRLNIQLGTAYLRDQIDKFGRIEYVAAAYNAGPGRAVQWKASLPFEIDEW
                     AEAVPIKETKGYVQGVVRNRLQYLRLYDEKGNFRPEVGTRAVTASPVPGASPSIEANS
                     TIRKRRISGGPREE"
     gene            5531..>6284
                     /locus_tag="VFH91_04070"
     CDS             5531..>6284
                     /locus_tag="VFH91_04070"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862192.1"
                     /translation="MTYRTYALLLIPLFCSFALALPQTVALTIQSEPNAAVWIDEIRR
                     GTTNGEGNLGVKKLSPGRHSVRVRASGFKETTVPLLPGRRSLSIKLVPTIDRAELLFQ
                     QAETARETSTNQASREKAADLYREALALRNAYPAAHLGLARVLMDLSQFDKAHDEIAA
                     ARRTRPAYAEASAVEGRIYREAGFNDDAISSFRRAIREGNGFQPEAHVGLARVYEEKG
                     QFNEAIAEYKRAIDQLSESEPVIYQLLGAAYEK"
BASE COUNT         1598 a         1621 c         1638 g         1427 t
ORIGIN      
        1 gcagggcgcc gaagaggccg atggtatcga ctccttaact cgtgtcgtcc gcgaccagat
       61 cggacacggc gccgattgga tcaaagtcta cgccgattac cgctggggaa ctcaaggcgc
      121 cgcgcctact ttttcactgg aggagttgaa actcgcagtc gaaactgcaa agagtgcgaa
      181 cgttcctgtt gctgctcacg cgacaaccac tgagggaatg agacgcgcca ctctggccgg
      241 cgtggagaca attgaacatg gtgatggagg tacgccggaa gtctttcaac taatgaagga
      301 acgtggtgta gcgctctgtc cgacgctggc cgtagccgga gcaaacgctg aacgtaagaa
      361 ggcagttttc caggcggcgc ttaactcagg cgtaacaatc gccagcggaa gtgacgtcgg
      421 agtatttgcg catggcgaca atgtccgtga acttgaagcg atggtcaact ttggaatgtc
      481 ggcgctggcg gctctgaaga gtgcgacttc agtggacgct cgcgtcttac atatggctga
      541 gaaaattgga tcagtaaagt cgggactcct ggcagacttg attgcagtgg aaggagatcc
      601 aacacaagac atttcgacgc tgcgcaaagt gagattagtt atgaagggtg gaacaatcta
      661 taagacgccg tgacggtggg aacacgttgt tagaccgttt ggcttgccac cagccttgcc
      721 accgtcgcga gcaattcttc agtcttgaaa ggtttagaaa cgaaagcact gaagcctact
      781 tgcagcgctc gaccgcgatc gagatcggta cgagctgtga cggcaacgca cggtatgtca
      841 gctgttgagg agtcggcctt cagctctcga aacacttccc acccatcgag ttcaggcatc
      901 gacagatcaa ccataataag atctggaggg ttttgtcggg ccaagttgag acactcctgt
      961 ccattacgag cttcacgaac cttgtaattt gccgcggaca agagcaagcg caacagctcg
     1021 cggttatcgt cgtaatcttc tgcaattagg atcgtggcag acatctctgt tacctgagtg
     1081 agaaccgacc cacagtgttt taaaaagcgc cctaagagta tcagaccttt attattaagc
     1141 aagatctgtg caacgagcag acggaagggt gagaaaaaca tagccattta tgctctatta
     1201 aattcaaact tcgagagcat ttctgcaatt tgtggggctt ttggagcctt actattccaa
     1261 aaacagctgc cagaatggcc agtcgcacga ctcaaaatcg atgggagccg gcaggaagag
     1321 tccgatttga agccacattc gagcgtttgg attagatact ttcgtagaca aaatgcaaaa
     1381 acaaattgac tagcgtaata gcagccgttc ataatcgctc tgagccctgc ccccatgcga
     1441 ggctctcggt aacccttggt tcagatccct accttagaag accttgaggt tcgtcaaaaa
     1501 aacaacacca cccacacgga ggaacgaact gtggtcaacg gacaattaga gaacagttta
     1561 cgccacgaga tcgagacgta cctcgaaagt cgtctgagcg cgatgaaaca ggacattagc
     1621 gcgctccaga gcctgctcaa tgaatccctt tccggcttgg tcgaacgaca gagcgatgtg
     1681 cccatggaag gtagtttaag caccgcgata tccgaacact tacgtgcggc tcacgaacgt
     1741 gggattgagc tcgccgcctc ggaatcttcc agagctaaag cctcgagtga tatggctatc
     1801 gtgaaagctg gaatcaccga gatcaacgaa caaggcaacc agtccgagat tctcaaagcc
     1861 ctcattaatc gcgcggcatc gtttgcgccc cgcgttgctt ttttcgtcat taaaggtgac
     1921 cgggcatctg gctggcgtgg tcgcggcttt gaaggaacgg ttggagacaa ttccatccag
     1981 gagatttcgg tctcgctctc ttctgacacg gtgttgggaa gtgcagccaa atcgctgacc
     2041 agctggagcg gtggtcccgg atcgcattca gaagatcatc tggtgctcaa tcggttaggt
     2101 gaagaaccct ctcaaagaat agttgcgatt ccgctgatcg ttcgtgggcg agcggtagca
     2161 gttttgtatg ctgactcagc aggtttagat tccgaatcaa tcaatcttga agctttggag
     2221 accctcgtaa aagtaaccgg aatggctgtg gaactacttt ccgcgagaag tgctccgaaa
     2281 cgcgcgagtg agccagcagc ggcaaaagag ccggctcctt cttacagccc taccagcgat
     2341 tatcaggagt caacaccggc agcttcaagt ttcgacttct cctctctcaa agaagctgaa
     2401 ccggcacaga gttcctatga ggagctacca ccggccgaga tgtccgttgt tgaagaagcg
     2461 ccggctgctg gttatgacat agctccagta tctgagtcgt ttactgaaac tccttcgtgg
     2521 agagacgaaa gtcctgcgtc atcgtctttt gaacccgaag aatcaaaacc aattgctccg
     2581 cctccaagcc aaacgaaacg gcgttatggt cacgaaacgg aactaccggt tgaggtaagc
     2641 agtgatgaag agcgtcgact acacaacgat gccttacgtt tcgcaagact gctgatctcc
     2701 gagatcaaac tttacaacga acaaaaagtc gtcgaaggtc gctctgaaca cgatctttat
     2761 gatcgcctgc gggagtacat cgatcgttca agagaaatgt acgacaagcg agttaaaccg
     2821 gaagttgctt cacgctacga ttactttcac ggtgagctgg taaacacgct tgcagaaggt
     2881 gacgtctcga agctcggatc gaactatccc ggcgcaacag ttaacgctta acttggagtg
     2941 cgctggcccg gccgcgcttt gttatataac tattcgcttt caggcgattc ccgatcttag
     3001 tttccaagca accgtaatca tttaacgacc cactaagaca ccagtccgtt gcgccccggc
     3061 cttcggaccg gagtgacttt tcatcgcgtt gttatagaat cactctcgtt ctcaaagttt
     3121 gttcaaagcg gtgacgactc gccgcgctta aaaattgtgg gtaaacatcg attagttatt
     3181 gtcgttctcg gtgtcagtgt acttgctgtc gcttcacttt ccttcctgct tagcgcgagc
     3241 tgtcttactc ggccgcaaac tctcggagag caaaaagcgc tcgaacagat gcgttcaatg
     3301 acgcgcaacg acgtccttcc gtcggaagac gccgtgctgc gtatcgagaa ccaattcaaa
     3361 ggaaccaagg cggcagctct cgctcgcctt ttgcgtgctc gcgttaagtt gaatgctaag
     3421 gatttcgcgg gtgctgccga tttactgcaa tcaaaaacca ttaacgagca aactgcgctc
     3481 ggcgactacg cgctttatct acgtggcact gcgctggaag atggaaaccg gcaagttgaa
     3541 gcacgggcca catatcagca acttctacaa agctttccca cttcacttcg tgcacgtgac
     3601 gctgccttgc gcgctgcaaa tctcctttcc aaaagcggtg agtcggcagc cattccgctg
     3661 ttgctaagag actttaatca agctgacgaa cccgcagcgt tgttagccac tgctaaggcg
     3721 tacgaacaaa cttcagacca aactcgagcg ctcgcaacat atcggcgatt gtatttcttt
     3781 gcaccttcga gcacggagag tgctgaagcc gcaacagcta tccctcgact cggatcaact
     3841 acgtcgccag ccactgttga tgaagccatt acacgggctg gtcttttgta cgatgcgagg
     3901 cgtttcgtcg acgcaattaa cgcgtacggc gaggcctttg ccaggttccc cgcgacggtc
     3961 actacagaaa accaacttcg gcgcggtatc gctgcctcga atgcccgaaa agttcccgag
     4021 gcagtcgcag ctctttcgag cattcccact tctgcaggtg aagcgagagc agaaggcctc
     4081 ttttatctgg cccaaactta tgcacgagca aggcaatggg aacaggcgcg ctcaacagtt
     4141 gaagaattac gacgctcatt tccatccagc aattttactc cccgagcctt tgtatcagtt
     4201 ggacagatag ctgaagacgc taagaatcct accgacgctt cttatttttt caagactgcg
     4261 gtgaatagtt tttccaattc gatcgaggtt gctcaggcac agtttgagat agcgtgggcc
     4321 gctcatgagt ccaggaactt cgctgactcg tcaaagatgc tgacagagca tctcgctttt
     4381 tatgcggata gaaacactga caatcgagga cgtgctgggt attgggcggc ccgtgattca
     4441 gaacgtgccg gtaagctcgc tgaagcccga gcgttgtatg aagcaatgca agctcgttac
     4501 gacgcgaatt ggtatggcta ccttgccaag caacgtctcg acgtaatcag ccgctcagcg
     4561 ccggcaaaga attttccaga cgattcgatt gttgctcgag ctgtcgcgaa cctcaagacc
     4621 gttaccgtag ctgaagaaac agcgggacct gaagaagaag ctcgcatcct gaaagcggat
     4681 cagctgtcca acgtcggact cgatgactgg gcaataaaag agttggataa agcgggcgag
     4741 aaacatcagg atagtccaaa gatcaacctg gctgaagcgc gcatctatcg ttccgacgat
     4801 gataatgttc gcgctctaaa cactctgcga cgaagttttc ctgactattc ccagatgaag
     4861 cccgaagaga tgacgcgcga agagtgggat gttttctacc ctcttgcgta ctgggacatc
     4921 attgtgcagg agtctcgcaa tcgtaatctc gatccttatc aagtcgctgg tttgatccgg
     4981 caggaaacga tcttcagtgc gcgggcacgt tcatcggcaa atgcgtatgg cctaatgcaa
     5041 gtgctagttc caacaggaaa gctcaccgct cgaaagtacg gaatgggtcg agaggtaacc
     5101 gccgactcga tcctagaacc acgattgaac atacagttgg gtaccgcata cttgcgggat
     5161 caaattgata agttcggaag aatcgaatac gtggcagccg catacaatgc aggtccgggc
     5221 cgagctgttc aatggaaagc ttcgctgcct tttgaaatcg atgagtgggc tgaggcagtg
     5281 cccattaaag agacgaaggg ttacgtccag ggcgtggtga gaaatcggct tcagtatttg
     5341 cgtctctatg atgaaaaagg aaactttcga cctgaggtcg gaacgcgagc tgtgacagct
     5401 tcgccggtgc ctggagcttc tccttcaatt gaggcgaact cgaccatcag gaagcgaagg
     5461 atttctggcg gaccccggga agaataatag gacctatatg acctataaga cctataggaa
     5521 taggacctat atgacctata ggacctatgc tctattgtta atccccttat tctgcagttt
     5581 tgccctggcc cttccccaaa ctgttgcttt aaccattcag tcagaaccga acgctgcggt
     5641 gtggattgac gagatccggc gtggcactac caacggcgag ggaaatctag gagttaagaa
     5701 actctctccc ggacgtcatt cggttcgagt cagagccagc ggttttaaag agaccacagt
     5761 tccgttgctt cccggacgtc gttctctttc catcaaactt gttccgacta tcgatcgcgc
     5821 cgagcttcta tttcaacagg ccgaaacagc ccgcgagacc tcgaccaacc aggcttcacg
     5881 cgaaaaagcc gccgatctct atcgcgaagc cctggcttta cgaaacgctt atcccgctgc
     5941 gcatctagga ctcgcgagag ttcttatgga cctcagccag tttgacaaag ctcatgatga
     6001 aattgccgcc gctcgacgca ctcgacctgc ctacgctgag gcatctgccg ttgagggtcg
     6061 catttatcgc gaagctggct ttaacgacga cgccatcagc tcgtttcgtc gcgctatcag
     6121 agaaggaaac ggtttccaac cagaagctca tgttggcctg gcgcgcgtgt atgaggagaa
     6181 gggacaattt aacgaggcca tcgccgaata taaaagggcc atagaccaac tgtctgagag
     6241 cgaacccgta atctatcagc tgctcggagc tgcatacgag aaga
//
LOCUS       DASSFP010000188         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_198484, whole genome shotgun sequence.
ACCESSION   DASSFP010000188 DASSFP010000000
VERSION     DASSFP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198484"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..558
                     /locus_tag="VFH91_04075"
     CDS             220..558
                     /locus_tag="VFH91_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET6862193.1"
                     /translation="MNRILTLTLLVFCVFTTGVMAQNSSTATKAGTKQSTKRGPVFRA
                     TKDQINQAQSLLKSRGFYAGEQIGKLDDATREGLRKYQQAEGLKVTGTLNKLTLEKMN
                     IQLTEKQKQM"
     gene            complement(616..3315)
                     /gene="alaS"
                     /locus_tag="VFH91_04080"
     CDS             complement(616..3315)
                     /gene="alaS"
                     /locus_tag="VFH91_04080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET6862194.1"
                     /translation="MTGDEIREAFLRYFEEHGHTRVRSSPLLPANDPTLLFTNAGMNQ
                     FKDVFLGTEKREYVRAASSQKCIRAGGKHNDLDEVGKTARHHTFFEMLGNFSFGDYFK
                     EDAIKLAWDLLVNKLKLDPARLWFTVYGGDEEVSPDEDAERLWQQVGAPAERVLRFGR
                     KDNFWQMGETGPCGPCSEVHYYMGPSPSDPENSAAYVNGPGDTIMEIWNLVFMQFDRS
                     EVEPGKYKLDALPAPSVDTGAGLERLAVVLQGVKSNYDTDLIRPIIEFTASLADRNYE
                     PETQEGFAMRVIADHARATAFAMADGILPGNEGRNYVLRKIMRRAIYQGHHTLNFEDL
                     FFNKVTNFVVDKMKDNYPELETQRDFIQKMVRLEEERFGSTLTVGLNKLDELFATTKG
                     SMPEFKALARLYDTFGTPRDLIRVGLEERGFTIDEVDFNERFDAALQELQQQGTSERV
                     EAKSKANPIYADLVKRLGRTQFRGYETTQLPEVKIVALLKGGQEVAAVEEDDEGEVVL
                     DQTPFYAESGGQVGDTGQLTTSDASAIVLDTYSPAQGLVVHKIRVERGSLSAGQKVSA
                     QVDVETRDATRRNHTATHLMHAALREVLGTHVKQAGSVVAPTYLRFDFSHYQPLTNDE
                     KEEIERLVNYHILRNEPVQTDEMEIEEAMRSGAMALFGEKYGEKVRVLSIKGVEAVFS
                     KELCGGTHVRATGDIGLFKITSDESIASGVRRIRAVTGVDAYERFRESEILVDELSSG
                     LRTSRGDLPATIHRLQDELKKARRESEELRLRIASGAVGSSSNGDDSRDIAGVKVLAR
                     EASGLDAAGMRQLSDTLLDRIKSGVVVLGRSSDGKVSLIVRTSSDLTGKVPAGQVIKE
                     LAPIVGGRGGGKADMAEGGGSQPEKLTQALEESFGVVERLLNK"
BASE COUNT          814 a          960 c          767 g          881 t
ORIGIN      
        1 agtgccgctc tgcccccttc ccaactgcat gatctggaaa gtacagacat ggcccgctct
       61 gcccccttcc aactgcatga ttctggaagt gacaattcac catttcgtaa aacaccctga
      121 ctattgacat cgatagtatg attaggcggt tttcgaaccg acctcacaag ttaatctatt
      181 aacccgcgcg ctccagcgcg cctcacaaaa ggaaaaagta tgaatcgaat actcacactc
      241 actctacttg tgttctgtgt cttcaccact ggagtcatgg ctcaaaattc aagcactgcg
      301 actaaagcag gaaccaaaca atcgaccaaa cgcggacccg tcttccgggc gaccaaggac
      361 cagatcaacc aggcccaatc gctacttaaa tcacgcggtt tctatgcagg tgaacagatc
      421 ggaaagttag acgacgcaac gcgcgaaggg ctgaggaagt atcaacaagc ggaaggtcta
      481 aaagttactg gaacgcttaa caaacttacc ctcgagaaga tgaacatcca actcactgag
      541 aagcagaaac aaatgtagct tccctttagg agtgcgtcgg ccctcggctg ttagccgtta
      601 agcagacgca ctcacttatt tatttagaag ccgttcaaca acgccaaagc tttcttctaa
      661 tgcttgcgtt agtttttccg gctggctacc gcctccttcg gccatgtcgg cttttccacc
      721 gccacgtcct ccgacgatgg gcgctagctc tttgataacc tgcccggcag ggacttttcc
      781 ggtgagatcc gacgatgttc taacaatcag cgaaacctta ccatcactac tccggcccag
      841 gactacaact cctgacttta tccgatccaa caacgtatcg gagagttgtc gcatgcccgc
      901 ggcatcgagg ccgcttgctt cacgtgccag cacttttact cctgcgatgt cgcgtgaatc
      961 atcgccatta gaagatgaac caactgcgcc actggcaatt cgcaaacgaa gctcttcgga
     1021 ttctcgtcgc gctttcttta actcatcttg caatcgatga attgttgccg gcagatctcc
     1081 gcgtgacgtt ctcaacccac tgcttagttc atccactagt atctctgact cgcgaaaacg
     1141 ctcgtaggca tccactccgg ttactgccct gatacgtcga actcctgaag caattgactc
     1201 gtcactcgta atcttgaaca gaccgatatc tcccgtcgcg cgtacgtgag ttcccccaca
     1261 caattcttta gaaaagactg cttcaactcc ctttattgat agaactcgaa ccttctcgcc
     1321 gtatttttct ccaaacaggg ccattgcccc tgagcgcata gcctcttcga tttccatttc
     1381 atcagtctgc accggttcgt tgcgcaggat gtgatagttg accagtcgct cgatctcttc
     1441 cttttcgtcg ttggtcaatg gttgatagtg cgaaaagtcg aatcgcagat aagttggagc
     1501 caccaccgag ccggcttgtt ttacgtgcgt gccgagcact tctcgcaacg cagcatgcat
     1561 caggtgagtc gccgtgtgat tacgtcgagt ggcatcgcgt gtctctacgt ctacttgagc
     1621 agaaaccttc tggccggccg aaagagatcc acgttcaacc cttattttgt gaactaccag
     1681 gccctgcgcc ggagaataag tgtctaacac gattgcggaa gcatccgagg tcgttagttg
     1741 tcccgtatcg ccaacttgtc cgccgctctc agcatagaaa ggcgtctgat caagaacgac
     1801 ttccccttca tcgtcttcct cgactgctgc gacttcctgt cctcctttta gtagcgcaac
     1861 gatcttcacc tcgggtaatt gcgtcgtttc ataacctcga aactgagttc ttcccaggcg
     1921 ttttacaaga tccgcgtaga taggattggc tttgctcttc gcctcaacgc gttcagatgt
     1981 gccctgctgc tgaagctcct gcagtgcggc gtcaaaccgc tcgttgaaat cgacctcatc
     2041 gattgtgaac ccgcgttctt ccaaaccaac tcgaattaaa tctctcggag ttccgaacgt
     2101 atcgtataga cgagcaagcg ccttgaactc gggcattgac cctttagtcg tagcaaacaa
     2161 ttcatccagc ttgttcaaac cgacggtgag tgtggatcca aaacgttctt cttcgaggcg
     2221 aaccatcttc tgaatgaaat cgcgctgagt ctcgagctcc ggataattat ccttcatctt
     2281 atccacgacg aagttggtta ccttgttgaa gaacagatcc tcgaagttga gagtatggtg
     2341 accctggtag attgcacggc gcataatctt ccgaaggaca taattccgtc cttcattacc
     2401 tggaagaatc ccgtcagcca tcgcgaatgc agtagcgcga gcatgatccg cgatcacgcg
     2461 cattgcaaat ccttcctgag tttcaggttc gtagttccga tcggcgagac tcgcggtgaa
     2521 ctcgataatt ggacggatca gatcggtatc gtaatttgat ttaacacctt gtagaaccac
     2581 tgcaaggcgc tcgaggcctg caccagtatc aacagagggc gcgggtagtg cgtcaagctt
     2641 atattttccc ggctcaactt cggatcgatc aaactgcatg aagaccagat tccagatctc
     2701 catgatcgta tcgccaggtc cgttgacgta agctgcagaa ttttcgggat cgctgggact
     2761 tgggcccatg taatagtgaa cttcactgca tggaccacat ggaccggtct cgcccatttg
     2821 ccagaagtta tcttttcgtc caaacctgag aactcgttca gctggagctc cgacttgctg
     2881 ccataagcgt tccgcatcct cgtccggcga aacctcttca tcaccgccat agactgtgaa
     2941 ccacaagcga gctggatcta atttgagttt gttgaccaac aagtcccagg ccaacttgat
     3001 tgcatcttcc ttgaaataat ccccgaagga aaaattcccc agcatttcaa agaaagtgtg
     3061 atggcgcgca gtctttccca cctcatcgag gtcattgtgt ttgccacccg cccggatgca
     3121 tttttgcgat gaagcagcgc gcacgtactc ccgcttttcc gttcccagaa agacatcttt
     3181 aaattggttc attcctgcgt tggtgaacaa taaggtcgga tcattcgcag gcaatagtgg
     3241 tgaagatcgg actctggtat gtccgtgttc ttcaaagtat ctaagaaatg cttctctaat
     3301 ctcgtcgcca gtcatacttt tgctttcaag ctcctcaaaa ccagtttagc acctttggag
     3361 tgcgccggcc tggcggcgct ttgttacatc agcttctcag ttacagagtt cgtgagatgt
     3421 gg
//
LOCUS       DASSFP010000189         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_198758, whole genome shotgun sequence.
ACCESSION   DASSFP010000189 DASSFP010000000
VERSION     DASSFP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198758"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFH91_04085"
     CDS             <1..1159
                     /locus_tag="VFH91_04085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6862195.1"
                     /translation="QASDDLRDAVLTRVVDSGPASSSLVGRLMFRAGWPQKSGIEYYI
                     QQHPVIMKEIEVRFRQAPEAIFHLEGEAMASALPWVSKQMRSLWSLHDLPSTVSEAST
                     RIACDADAREPTAAEIREARFAKNAERLFAEHARLVLCIADYDEVRLRNWGCRAVDYL
                     PLSIPDDDGVLTSRRWLPGGKLRLLHLGSVGHLPSYRSLEFMLDQVWPSLSSEAHERI
                     ILNVVGAADPGNQRTKRILSKAAPYSNVIFHGFVPDVRPYYEDSDLQIVASTDASGLR
                     TRTIESFAYGLPVLSSSIGARGIAKLEAGRDLIVADEPAEWINAITKILNRTEDLQTL
                     AVRARKFYDENNSRQAVAESLAHSLERHLDVQLNRLPDGHVDMRQPTSRTS"
     gene            1169..2167
                     /locus_tag="VFH91_04090"
     CDS             1169..2167
                     /locus_tag="VFH91_04090"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6862196.1"
                     /translation="MESSRPSSLDRNGSGVDLSLLVCTYNRAGDLRELLETAVAQETN
                     GEFSYEVVVVDNNSTDETRSVVESFIAAGHKNVRYFFEKKQGKANALNTGLRELKGWA
                     YVIADDDFVLPEDWVKNLYRGFRDNPEVSFVSGKVLPLFQGEPPSWLTAKHWSAIAMA
                     DYGDEQFVTDKENQICLLACAFRLADVESVGGYDKQLGPSKTHSGGTEDLDLLKRLWK
                     SGRKGLYLPNAAFYHKATKDRLTKDYHRRWHADHGRSYAIMRIEETEQAKFRLFDVPA
                     YMFREAARDTLRWVGCMIRGRTDDAFWHETQLRFFAGFVAKRRADSRGNRNAMQSQ"
     gene            2177..3586
                     /locus_tag="VFH91_04095"
     CDS             2177..3586
                     /locus_tag="VFH91_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862197.1"
                     /translation="MNANPESLNTTPASQRPRKGSLAGYVMLSGLQWLRGSRSLKLFR
                     QISREPFVSTEEVESSQLRRLSDLLSHAEQNVRYYRDLLKSLKITSRDIRSFSDFSQL
                     PVLTKEIVRERWRDLVREDVPLESLSKHYSGGSTGVPLTFYRDKEYMDASEAGTFRNF
                     AQAGWQPGEMIAFFWGSNERIQQMSRTQFELRQRLRRMYQFDPFHSGTDQLREWLDRW
                     RSLDATIALGYSSTVARFAQFIESSGIKVEPLRGVFTTAEKLYGPQREVIERVFGCRA
                     FDCYGSSEVQNIAAECPAGSMHINADYVYLESEENPGVADAAPFLVTSLKNYAMPFIR
                     YRNEDCGSLLEGTCGCGNNFPLMRLNVARVSDNFVFPDGKVVHGEFFTHLMYGSTGID
                     TFQFHQTSPDHITLWIVPGEGDAESRRRTIDSAIKQIKGLSGAPVNVEIREVPSIPLS
                     NAGKHRFTRSDVGDSATLS"
     gene            3591..>3885
                     /locus_tag="VFH91_04100"
     CDS             3591..>3885
                     /locus_tag="VFH91_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862198.1"
                     /translation="MCGICGIHTNSFVTPDDERVVRDMMSLLDHRGPDGDGIWSSKTG
                     AVLGHRRLAIIDLLGGHQPLFNETRDVGVVLNGEIYNYRELRQELTSFGHTFVT"
BASE COUNT          941 a          876 c         1066 g         1002 t
ORIGIN      
        1 ccaagcaagt gatgatctaa gagatgcggt tttgacccgg gtggtcgatt ccggacctgc
       61 atcgtcatcg ttagttggcc ggcttatgtt tcgagctggc tggcctcaga agagtggtat
      121 cgaatactac atccaacaac atcctgtgat aatgaaggaa attgaggtca gattccggca
      181 ggcccccgag gccatttttc atttagaggg cgaggcgatg gcgagtgcct taccatgggt
      241 ttcgaagcag atgcgaagcc tctggagctt gcatgatctt ccttcaactg tttcggaggc
      301 gtcgaccagg attgcctgtg atgctgacgc tcgcgagcca actgctgctg agattcgaga
      361 agctcgattt gcaaaaaatg cggaacgcct ctttgccgag cacgcacgtc tcgtcttgtg
      421 cattgcggat tacgatgagg ttcgtctccg caactgggga tgtagagcgg ttgattattt
      481 gccgctgagc attcccgatg acgatggggt cttgacttca agaaggtggc tgcccggagg
      541 caagttaaga ctgttacatc tgggcagcgt gggccattta ccaagttacc gctcacttga
      601 gttcatgttg gaccaggtgt ggcccagtct atcgtcggaa gctcacgagc gaattatcct
      661 gaatgtcgtt ggagcggcag atcccggaaa tcaacgtacg aaaagaattc tttctaaagc
      721 ggcgccttat tcaaatgtga tcttccatgg ttttgtaccg gatgttcggc cttactatga
      781 ggacagcgat cttcagatcg tggcatcaac cgatgcgagt gggctccgaa ctcgtactat
      841 cgagtcattt gcctatggac ttccggtgtt aagtagttct attggcgcgc gtgggatagc
      901 gaaactagaa gcgggccgag atttgattgt ggctgatgag ccggcggaat ggataaatgc
      961 aattacgaaa atcctcaacc ggaccgaaga ccttcaaact ctcgcggtaa gggctcgcaa
     1021 gttttatgac gaaaataaca gccggcaagc ggtggctgaa tcgttggcac attctttaga
     1081 acgacatctt gacgttcaac taaatcggtt gcctgatggt catgttgata tgaggcagcc
     1141 aacatcaaga acgagttagt cgagcaatat ggaaagttct cgtccatcgt cattggatcg
     1201 taacgggtca ggagtcgacc tttcactgtt ggtgtgtacc tataacagag ccggcgatct
     1261 tcgcgagttg cttgaaaccg ctgttgctca ggagaccaac ggcgaattta gctatgaagt
     1321 tgtcgtggtg gacaataact cgactgacga aacacgtagt gtcgttgaaa gctttatagc
     1381 agcgggacac aagaacgttc gctacttttt tgagaaaaaa caaggcaagg caaacgccct
     1441 aaacacgggt ctgcgtgaac tgaaaggttg ggcctacgta attgctgatg acgatttcgt
     1501 attacctgag gattgggtaa agaatcttta tcgggggttt cgagacaatc cggaagtctc
     1561 ttttgttagt ggcaaggttt taccgttatt tcagggggaa ccaccttcgt ggttgaccgc
     1621 caaacattgg tctgccatag ccatggccga ttacggtgat gaacagtttg ttaccgacaa
     1681 agaaaaccag atatgtttgt tggcatgtgc ttttagatta gctgacgttg agtcggttgg
     1741 aggttatgac aaacagttag gcccaagtaa aacgcactcc ggcggcacgg aagatttaga
     1801 cctgctgaag agactctgga aatcgggccg caaaggtctc tatctaccaa atgcagcgtt
     1861 ttaccataag gcaaccaagg atcggctaac caaggattat catcgacgct ggcatgctga
     1921 ccatggccgt tcctacgcaa tcatgcgtat cgaggagact gagcaggcaa agtttcgtct
     1981 ttttgatgtg ccggcttata tgtttcgcga agcagctcgg gacacgctgc ggtgggtagg
     2041 ttgtatgatt cgaggtcgaa ctgacgacgc attctggcac gaaacgcagt tgaggttttt
     2101 cgccggattc gtcgccaaga ggcgtgctga ttcacgtgga aacagaaacg caatgcagtc
     2161 gcagtagtat ccgataatga atgcgaaccc cgagagcttg aataccacgc cggccagtca
     2221 acggccgcgt aagggtagcc ttgcgggtta cgtgatgttg tcgggcctgc aatggctccg
     2281 tggttcacga agtcttaaac tgtttcgcca gatctcgcgc gagccttttg tctcaacaga
     2341 ggaagttgag agtagtcagt tgcgacggct ctccgatttg ttgtcccacg ccgaacaaaa
     2401 cgtccgttac tatcgcgacc tcttaaagtc tctcaaaata actagtcgcg acattcgtag
     2461 cttttctgat ttcagccagc ttccagtgct gactaaggag atcgtacgcg agcgatggcg
     2521 agatctcgtc agggaagatg tgcctttgga aagtctatcg aaacattaca gcggcggttc
     2581 taccggagta ccactgacct tttatcgcga caaagaatat atggacgcgt ctgaagctgg
     2641 tacgttccgg aactttgcgc aggccggctg gcaacccggc gagatgattg ctttcttctg
     2701 gggcagcaat gaacgcattc aacagatgtc gcggacccaa ttcgaacttc gacagcgact
     2761 acgaagaatg taccagtttg atcctttcca ttctggtact gatcagctgc gcgaatggtt
     2821 agaccgttgg cgttccttgg atgccaccat tgcgctcgga tactcgtcga ctgtagctcg
     2881 atttgcacaa ttcattgagt cgagcggtat caaggttgag ccactgaggg gagttttcac
     2941 cacggctgaa aaactctatg gaccgcaacg cgaagtaatc gaacgcgttt ttggttgcag
     3001 ggcgttcgat tgttacggaa gcagtgaagt gcaaaatatc gccgccgagt gtccagccgg
     3061 cagtatgcac attaatgcgg attacgttta cctggagtcc gaggaaaatc ccggtgtcgc
     3121 ggacgctgca ccattcctgg ttacttcgct caagaattac gcgatgccgt ttatacgtta
     3181 tcgcaacgaa gattgtggaa gcctgcttga aggaacgtgt ggatgtggga ataactttcc
     3241 gttaatgcga ctgaacgttg ctcgggttag tgataatttt gtttttccgg atggcaaagt
     3301 ggtgcatggc gagtttttta ctcaccttat gtatggcagt actggaatcg atacgtttca
     3361 gtttcaccaa acgagtccgg atcacattac gctttggatc gttcccgggg aaggtgatgc
     3421 ggaatcgcgc cgtcgaacaa tcgatagcgc catcaaacag atcaaaggat taagcggagc
     3481 gcccgtgaac gttgaaattc gtgaagttcc gtcgatcccg ctttcaaatg ccggtaaaca
     3541 tcgatttact cgttcagacg ttggtgacag tgcaacctta agttgattct atgtgcggaa
     3601 tttgcggaat tcatactaat tcatttgtca ctccggacga cgaaagagtc gtgcgtgaca
     3661 tgatgtcatt gttggaccac cgtggtcctg atggtgacgg aatctggtct agcaagaccg
     3721 gggcggtgtt gggtcaccgt cgtctggcaa ttatcgactt attaggtggt caccaacctc
     3781 tgttcaatga aactcgtgat gttggtgttg ttctaaatgg cgagatttac aactatcgag
     3841 agttgcgaca ggagctaact tcctttggtc atacgtttgt aactg
//
LOCUS       DASSFP010000190         5727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_198822, whole genome shotgun sequence.
ACCESSION   DASSFP010000190 DASSFP010000000
VERSION     DASSFP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198822"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1514
                     /locus_tag="VFH91_04105"
     CDS             141..1514
                     /locus_tag="VFH91_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET6862199.1"
                     /translation="MEFKENVLGLIGQTPLVKLNRLARGVNAQIFAKMESLNPGYSVK
                     DRIGVAMIEAAERDGLLKPGGTVIEATSGNTGIGLALAAAVKGYKCIFVMTDKASVEK
                     SRYLKALGADVVITPVSAKPGTPDHYVSTARRIASETPNSFYPDQYSNPSNPDAHYRT
                     TGPELWEQTGGKITHFVAGIGTGGTISGTGKFLKEKNPAIRIIGADPYGSIFKTFKET
                     GKLVEATPYLVEGIGQEIVPPNVHMKYIDEVVNVTDSESFEMSRMLGRTEGIFCGGST
                     GTNLGAALRVAKDLDEEAIVVFIVCDTGEHYLSKHHSDEWLKEKRLLEPQRMTAGLIS
                     GTKKPQAPKTLIYVNPKDTVAEALEKMDELGLTQVPVLDEGRSVGSLRENRVLAKVVR
                     NRDMLTLPVSEVMELSFPILDVDANSSEVTKRLQTSPAVLVEEYGRIIGIITRHDVLD
                     LRMKSSV"
     gene            1525..1986
                     /gene="smpB"
                     /locus_tag="VFH91_04110"
     CDS             1525..1986
                     /gene="smpB"
                     /locus_tag="VFH91_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET6862200.1"
                     /translation="MTKEEKVLAANRAAFHNYHISDKYEAGVALTGTEVKSAMEGRVQ
                     LKEAYVGVKDGEAWLFNAHISPYSHGNRENHEPLRTRKLLLHKREISRLDEASARQGM
                     TLVPTQLYLKNGRIKVEVGVARGKKLYDKRETELKRTVERETRAQLKERNR"
     gene            2030..3529
                     /locus_tag="VFH91_04115"
     CDS             2030..3529
                     /locus_tag="VFH91_04115"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET6862201.1"
                     /translation="MQVESTQRPYEDLDVQALAVAVFKGEKTDDGFLKKLDDLSAGLV
                     KSAIDSEEFAGKEGETAYFHLVGNNKLKAKRLMLVGVGEKADYKAGQLSQMTGTAVRA
                     LRSKNSKSIAVIPRPANADGEMAAAAVEGAYISLFDPDKYRTVEKEQKTVERLVVVFD
                     GGADDKLQHGVEAGKIIGESINFTRDLANEPGAYMTPTDMAERAREVANEFGLNIDVL
                     DEARMEQEGMGSLLSVTRGSEEPAKLIILKYTPAGFDESNKDLLAFVGKGVTFDSGGI
                     SLKPGENMELMKYDMTGGATVIGVMRAIAQLKPPIPILGVAPCTENLPSGKATKPGDV
                     VRAMTGKTIEIINTDAEGRLILADAIAYARKLGATKVIDMATLTGAVSIALGDVNAAV
                     LGTDQELIDEIIAAGQEVGEKFWQLPLDKEYTKQIKSDIADIKNVGGRKAGTITAAAF
                     LKEFADGLSWAHLDIAGTAWGDDAKPYRSKGPTGIAVRTLLRIVERASK"
     gene            3913..4728
                     /locus_tag="VFH91_04120"
     CDS             3913..4728
                     /locus_tag="VFH91_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET6862202.1"
                     /translation="MIDLYKILGVKANASQAEIKSAYRKLARKSHPDLNPDSQAAREF
                     SLLSKAYHTLSDPQERAYYDDQWKAQKNRSYSILSSNNPHAKRARNLAVQAKWDRLVD
                     EVLERDRRENRERQRAVFTTVSLFLSTFFLAMIRPQLWQVFDYLGRAIVLTLFVIGVW
                     HLATRLREYLAHYTYQPKPILGSIIHQDELPEQPFTRFSAYTFLLVGYALSLGIGLYI
                     GWHTQDFFNDLTLLFRHRVAVVQSTLLIPDLIVYPPIAVLIVDTVHGLASRFD"
     gene            4958..5224
                     /locus_tag="VFH91_04125"
     CDS             4958..5224
                     /locus_tag="VFH91_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862203.1"
                     /translation="MGNTMSQRQSLASKLDALSETELQEVLDYVSMIESMRRSMGATT
                     GNEEELLTTLADAHENRRARQAFEWESVRRKAERRAAVRASSHF"
     gene            5225..>5727
                     /locus_tag="VFH91_04130"
     CDS             5225..>5727
                     /locus_tag="VFH91_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6862204.1"
                     /translation="MPKRSLISFAVATAVIILTSVVTGQQSPSNLARTQLELERQQQR
                     LHSSDPEERRDAVMRLGSMRTASASRLVLPLLSDTVPLVRAVAARAILSLGPDESASA
                     LLPLLNDKDEFVRREAAYALGWTHNRNASQPLGNLLLTDKEDGVRSAAAVALGEIKDE
                     SAVVVLAS"
BASE COUNT         1497 a         1342 c         1534 g         1354 t
ORIGIN      
        1 ggtcactgga atctcgatgg acatcgagta gcaggcgacc ttttggcgca aaaactatgc
       61 accgggggct ggctgaaata gcagccgaga cgcgtttctg ttatagatag tgctacttct
      121 ttgtgctccg gagagtgtgg atggaattta aagagaatgt cctcggacta attgggcaaa
      181 cgccactggt caagctcaat cgtcttgctc gaggagtaaa cgcccagatt tttgccaaga
      241 tggaaagtct aaatccggga tattcggtaa aagaccgcat cggcgtggcg atgattgaag
      301 ctgcagagcg agatggctta cttaaacccg gcggcacagt cattgaagct acttccggta
      361 acacaggaat cggcctggcg ttggcggccg ctgtaaaagg ttataagtgc atattcgtga
      421 tgactgataa ggcttcagta gagaagtcgc gctatttgaa agccctcggc gccgatgtcg
      481 ttattacacc agtttcagct aaacccggga cgcccgatca ttacgtttca accgcacgac
      541 gaatcgcttc tgagactccc aattctttct accctgatca gtattccaac ccatccaatc
      601 ctgacgcgca ttacagaact accggaccgg aactttggga acaaaccgga ggaaagatca
      661 cccattttgt tgccggcatt ggtacgggag gaactatttc gggaacggga aaatttttga
      721 aagaaaagaa ccctgcaatc agaatcatcg gcgccgatcc ctacggttca atcttcaaga
      781 cgttcaaaga gacaggcaag ttggtcgaag caactcctta ccttgtagag ggcatcggtc
      841 aggaaatagt tccgcccaat gtccatatga aatatatcga tgaagttgtg aacgtcactg
      901 acagcgaatc gttcgaaatg tcgcgtatgc tgggtcgtac ggagggcatt ttctgcggcg
      961 ggtcaacagg gaccaatctc ggtgccgcac tccgcgttgc gaaggatctc gatgaagaag
     1021 caatcgtggt gtttatcgtt tgcgataccg gtgaacatta tcttagtaag catcactcag
     1081 acgaatggct gaaggaaaag agacttcttg aaccgcagcg gatgaccgcc ggtttgatca
     1141 gtggaacaaa gaagccccag gcgccgaaga cgcttattta tgttaatccg aaagatactg
     1201 ttgccgaggc actcgaaaag atggacgaac tcggtctaac gcaggttccg gttctcgatg
     1261 aaggccgatc ggttggatct ctgagagaaa atagagtgct ggcaaaggtg gttcgcaacc
     1321 gcgacatgtt gacgttacct gttagcgagg tgatggagtt gagtttcccc attttggatg
     1381 tggatgccaa ttcgagtgag gtaaccaaaa ggcttcaaac tagtccggca gtgcttgttg
     1441 aagagtacgg tcgcatcatt ggaatcatca cccgtcacga tgtgttggat ttacgcatga
     1501 agtcatccgt ttgatacctg cagcatgacc aaagaagaaa aagtgttggc tgcaaatcga
     1561 gctgcctttc ataactacca tatatccgat aagtacgaag ccggagttgc acttactggt
     1621 acagaagtaa aaagcgcgat ggaaggtcgc gttcagctaa aagaagcgta tgttggagta
     1681 aaggatgggg aagcctggct gtttaatgct cacatctctc cttactctca cggcaatcgc
     1741 gaaaaccatg agcctctgcg aacgcgcaag ctactcttac ataaacgaga aatatcccga
     1801 ctcgacgagg cgtcagctcg tcaaggaatg accctggtcc cgactcaact ctacttgaag
     1861 aatggaagaa ttaaggtaga agttggagta gctcgtggca agaaactgta cgacaagaga
     1921 gagacggagt tgaagcgtac agtagaacgt gaaacgcgag ctcagcttaa agaacgtaat
     1981 cgataacaga tttacaattc atatcttaat cgcaggagac tttagtcaca tgcaagttga
     2041 atcaacgcag cgtccttacg aagaccttga tgtgcaggct ttggctgtgg ctgtatttaa
     2101 aggtgagaaa acggacgatg gcttcttaaa aaaactggat gacctttctg ccggattggt
     2161 gaagtcggcg atcgactctg aggaatttgc gggcaaagaa ggcgagaccg cttattttca
     2221 tttagtcggt aacaataagt taaaagcaaa acgcttgatg ttggttggcg taggagaaaa
     2281 agccgactac aaagcagggc agttgtcaca gatgactgga accgcagtgc gggcgctgcg
     2341 aagtaaaaat tcaaaatcca ttgctgtaat tcccaggccc gccaatgcag atggagagat
     2401 ggccgcagcg gcagtcgaag gcgcttacat ttctctattt gatcccgata agtatcgaac
     2461 agttgagaag gaacaaaaga cagtcgaacg gctcgtcgtt gtttttgacg gaggagcgga
     2521 cgacaaactc caacacggtg tcgaagcggg aaagatcatc ggagaatcaa tcaactttac
     2581 gcgtgacctt gccaacgagc ctggcgcata tatgacgccg accgatatgg cggaacgtgc
     2641 tcgagaagtc gcaaatgaat tcgggctgaa tattgatgtt ctggatgaag cgcgaatgga
     2701 acaggaggga atgggatctc ttctcagcgt gacccgcggt tcagaggaac cggcaaaatt
     2761 gatcatattg aagtacacgc ccgccggttt tgatgagagt aacaaagatc tactcgcgtt
     2821 tgtcggcaaa ggtgtgacct tcgattccgg tggtatttcg ctgaagcctg gcgaaaacat
     2881 ggaattgatg aagtacgaca tgaccggtgg cgccacagtt attggagtga tgcgtgccat
     2941 agctcagcta aaacctccga ttcccattct cggcgtggct ccgtgtactg aaaatcttcc
     3001 ttcgggcaaa gccacgaagc cgggtgatgt ggtcagagcg atgactggca aaacaatcga
     3061 aatcattaac acggacgcgg aaggtcgcct gattcttgct gacgcgatcg cctacgcaag
     3121 gaagcttggt gcgacaaaag ttatcgacat ggcaactttg acgggagccg tatcgattgc
     3181 tttgggcgat gtgaatgcag ctgtcctcgg tactgaccag gaattgattg atgagattat
     3241 tgctgccggt caggaggtcg gcgaaaagtt ctggcagcta cccctcgata aggaatacac
     3301 gaagcaaata aagtctgaca ttgctgatat caagaatgtt ggcggccgca aagccggtac
     3361 gatcacggca gcagcgtttt tgaaggaatt cgcggacggt ctatcatggg cgcatctgga
     3421 tattgcagga acggcctggg gtgacgatgc aaagccttac cggtccaaag gaccaactgg
     3481 cattgccgta aggaccctat tgcgaattgt cgaacgcgcc agcaagtgag tttcaggcgc
     3541 gcggtgtagt taatggtaga cagccagtgt cttctaatgt tgacactttg gggttgaggg
     3601 ccggaccgaa atccaaattt gctgggtttt tcggcgtaaa cgggtgatgg catgaggctt
     3661 gtaggggttg taggcaatca gccaaaggga gcgagtccct tatccagtaa cgtcctcgcc
     3721 ccctcgattg ccgctcgagg ttttaccggc cgcccctttg gcacctccac tcaaaaactc
     3781 ccaccataaa aagtctgaaa attgctggcg ctgcatcgtt ctgtgagtgt tataattttt
     3841 ttggccagct tggtgatctc ctcaggcagc cctggcctac atcgctggct ctaaaggatt
     3901 agcggaagca cagtgatcga cctttacaaa atactgggcg taaaagccaa cgcttcgcag
     3961 gccgaaatta aatcggctta tcgtaaactt gcgcgaaaaa gccacccgga ccttaatcct
     4021 gactctcaag cggcacgtga gttttcactt ctatccaagg cttatcacac cctcagcgat
     4081 ccccaggagc gagcctatta cgacgatcag tggaaagctc agaaaaatcg cagctactcg
     4141 atattaagtt ccaataaccc gcacgccaag cgagcacgca accttgccgt tcaggcaaaa
     4201 tgggaccgtc tggttgatga ggtacttgaa cgtgatcgcc gcgagaatcg cgaacgccaa
     4261 cgagctgtgt tcacgaccgt ctcgttgttt ctgtcgacgt tttttctggc catgattagg
     4321 cctcaactct ggcaggtctt cgattacctg ggccgggcga ttgtattgac gctttttgta
     4381 attggtgttt ggcatctggc tacccgactt cgcgagtatc tggcccatta cacttatcag
     4441 cctaaaccga ttctgggctc gattattcac caagacgagc tgcctgaaca gccgtttaca
     4501 agattttcag cgtacacatt cttgttggtt ggctacgctt taagcctcgg tataggtctt
     4561 tacatcggct ggcatacgca ggacttcttc aacgatttga cgcttttatt cagacatcgg
     4621 gtcgctgttg tacagtcgac tttattgatt ccggacctta tcgtctatcc tcccatagct
     4681 gttctaattg tagatacagt acacggactc gcctcgcggt ttgattagcg tccaagacct
     4741 cgcgttttct atggtcgcca aatcttgcct gagccgattt ttgtcgtgta tagtgctggc
     4801 ttcccagccg ttccaagagt gatagctgtc aacagtttaa gagacaactc tcgaactgac
     4861 gcgcccctac tctaaaagcg aacggccaaa cctcagcaat atggattcct tgaaaaccct
     4921 ggtttggcgg ttagttttgc tggaaaggag cactgacatg gggaacacga tgagtcagcg
     4981 tcaatccctg gcctcgaagc tcgatgcatt gtcggagact gagttacaag aggttctgga
     5041 ctatgtgtcc atgattgagt ccatgcgacg ttcaatgggc gcaactacgg gtaatgagga
     5101 agaacttttg actacactgg cggatgccca cgaaaacaga cgcgcgcgac aggcttttga
     5161 gtgggagtca gtccggcgaa aggctgagag acgagcggct gttagagcca gcagtcattt
     5221 ctgaatgcca aagcgatcac ttatctcatt tgcagtcgca acagcagtca taatattgac
     5281 gagtgttgtc accggacagc aaagtccgtc caatcttgcc agaactcagc tggaacttga
     5341 acggcagcaa cagcgacttc attcttccga tccagaggaa cgaagagacg cagtgatgcg
     5401 gctcggttcg atgcgtacag caagcgcttc aagactcgtt ttgcccttgc tttctgatac
     5461 cgtgccactt gtcagggccg tagcagccag agcaatcttg tcgttagggc cagacgagag
     5521 tgcttccgct ttactaccac tactaaatga caaagacgag tttgttcgcc gtgaggcggc
     5581 ttacgcgtta ggctggactc acaatcggaa cgcatcgcaa cctctcggaa accttttgct
     5641 gacagataag gaggacggtg tgcgcagcgc agcggcggtc gctctgggag agataaagga
     5701 cgaaagcgca gttgtggtgc tggcatc
//
LOCUS       DASSFP010000191         5476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1379835, whole genome shotgun sequence.
ACCESSION   DASSFP010000191 DASSFP010000000
VERSION     DASSFP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379835"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFH91_04135"
     CDS             <1..964
                     /locus_tag="VFH91_04135"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6862205.1"
                     /translation="YYRIFFVGIVLSLSGSVLMAQSAKTDQDRSVEAEQKTQVRLKLK
                     DGTYLNVDDAWESPQGIWYRQGSLSYLASKDRVKSIDRVGPSTNDSSDDNTATVPQPV
                     IAEVSDTEKPAWIYLKGGAKVEADSASESAAGVWYKRGPLSLFIERARLDHIERETAA
                     VAAGPKERGWSTGSQSLDRIIKQNGSRYGVDPYLVFLVMEQESHFNTRAVSPKGARGL
                     MQLMPGTSARFGVRRPHDAAQNVSGGTRYLKELLNRFNNRVDLVLASYNAGEGAVIKF
                     GHRVPPYRETRNYVKKISFRYNRGKDKAKPATQATSKNNSTGEQ"
     gene            964..1689
                     /locus_tag="VFH91_04140"
     CDS             964..1689
                     /locus_tag="VFH91_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862206.1"
                     /translation="MVKTSIRVRNYLCVFSIVFLVSSFYGSSVLAQGGNGPLNNGAVV
                     KLVKAGFKEKTIISIISSRPANFELETDQLIELKRNGVSENIILAMLSQSVGFATNDD
                     DWSSNDSFFKGKRPGLDPNSQSTNIFGSGASSGSSNKSRGREEGNENEGTVTGSATVR
                     IIRPPSEAGGSEPKLEKTPTLDNEAVIRLVEAGFSEGTIIKRIEGSPADFDLSPSKLD
                     DLHKRRVTDAIITAMTLAMNEKG"
     gene            2119..4158
                     /locus_tag="VFH91_04145"
     CDS             2119..4158
                     /locus_tag="VFH91_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6862207.1"
                     /translation="MRPIKHAERGLTFLAGRTFNLIQSVNKFRPNPSFTPKWSEKPLL
                     KSWQKSKPTLGWPRTTDSLCPNCVIEAREAILSGKKDVSVLINEKVGEIKAQIIERDG
                     EIWMIKDCPQHGHFEDMMAIDSKFLTHIEKMFPGRDIDAHNDEKLHNHGSSTVKYGRG
                     AVLTVDLTNRCNMMCDPCFMDANQVGFVHELSMDEIKEILDNAITIKPRRQMSVQFSG
                     GEPSISPHFLEAIKYSRKVGYNSVQAATNGIEFAKSKEFCREAAEAGLRYVYLQFDGI
                     GNDANSHRQVGNLFDVKMRAINNLHEAGVEIVLVTTLVNGINNDQVGSIIRFALDNPK
                     KIAFLSFQPVSFTGRDEEITEHRRLQQRYTLSHLAHDVKNQVGITEPTRDWFPLSLMG
                     AFADFADLVHGPEAEWGQVSCGCHPNCGVGTAVMIDKENKEMRPVPEFLNIPGLVKDM
                     QKITDAARGKWMSNVMMGLTLLKHYNPYKSPSQFTLYELFKKFDKSFGLTGKDYGRVG
                     GDRSKNDIEKRRSDRWNFLFIAGMWFQDLFNYDFRRTEMCIIPYGTQEGEISFCAYNT
                     GIGWRNIIENMHQNATVAKWYQEHGRHEIFAGGKDVQLADKAHGMILNEVDLARPNKP
                     QMEGPKTAHEEAVMMRKMYQEMVLNKQLDVKQADKPVQIKGLGRKKEEEVEAVLV"
     gene            4318..4521
                     /gene="rpsU"
                     /locus_tag="VFH91_04150"
     CDS             4318..4521
                     /gene="rpsU"
                     /locus_tag="VFH91_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET6862208.1"
                     /translation="MAIVTVNQGESIESALRRFKRKVMTEEIIKDAKKHAFFIPPGQK
                     AKLKSALARKRNKKKGRMRVPRD"
     gene            4668..5438
                     /gene="surE"
                     /locus_tag="VFH91_04155"
     CDS             4668..5438
                     /gene="surE"
                     /locus_tag="VFH91_04155"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET6862209.1"
                     /translation="MTRILVTNDDGIHSDGLIQLEQALKELGDVYVVAPASEMSGASH
                     SLTLARPLRIRQLDSRHWSIDGTPTDCVTLALNRILSENERPDICVSGINHGGNLGDD
                     ATYSGTVAGALEATILGVPGIALSLVARENFDFTEAAAFAVVAVRKVLEGHLPEGTLL
                     NINIPPGEIKGVKVARQGIKNARPVITEHIDPRGKPYFWIGEEYFNTNSVEGTDYHAV
                     QAGYISVTPLRSDMTDHQALTALETWNYLKDSELLQKS"
BASE COUNT         1483 a         1294 c         1433 g         1266 t
ORIGIN      
        1 ctattaccgg atcttttttg taggaattgt attgagttta agtggctcgg ttttgatggc
       61 gcagtctgca aaaaccgatc aggacaggtc tgttgaagct gagcaaaaaa cacaggtacg
      121 attgaagctc aaagacggca cgtacttaaa cgtcgatgac gcctgggaat cgccgcaagg
      181 tatttggtac agacagggaa gtttgagcta tctcgcttct aaggatcgtg ttaaatcgat
      241 cgatcgtgtt ggcccgagca caaacgacag ctctgatgac aatacggcaa ccgttccgca
      301 gcccgtgatc gctgaagtaa gcgatacgga gaagcctgca tggatctatc ttaagggcgg
      361 cgcaaaagtc gaagccgaca gcgcgtctga atccgccgct ggagtttggt ataaacgtgg
      421 accgctctct ctcttcatcg agcgtgctcg tcttgatcac atcgagcgtg agactgcggc
      481 ggtcgctgct ggaccaaaag aacgcggatg gagtaccggt agtcaatcac tcgaccggat
      541 tattaagcaa aacggtagca ggtatggagt tgatccgtat ctggttttcc tcgttatgga
      601 gcaagagtca catttcaata ctcgcgctgt ttctccgaaa ggcgcgcgcg ggctcatgca
      661 actgatgccc ggaacgagcg ccaggtttgg tgtgcgacgc cctcatgacg cggctcaaaa
      721 tgtttctgga ggcactcgct acctcaagga gttactgaat cgtttcaaca atcgagtaga
      781 tctcgtattg gccagttaca acgctgggga aggtgcggtg attaagtttg gtcatcgcgt
      841 accaccatat cgcgagactc gaaactacgt gaaaaagatc agctttcgat acaaccgcgg
      901 aaaagataaa gcgaaacctg ccacccaggc gacgtcgaaa aataattcca caggagagca
      961 gtgatggtta aaacgtcgat tagagtgcgc aattatttat gtgtgttctc gatagttttt
     1021 ctcgtcagtt ctttttatgg atcgagtgtt ttggctcaag gtggcaatgg tccactcaac
     1081 aatggagccg tagtaaaact ggtaaaggct ggttttaaag agaagacgat tatctcaatc
     1141 atcagtagcc ggcctgccaa ctttgaactc gaaacggatc agctgattga actgaaacgc
     1201 aatggtgtta gtgagaacat cattctcgcg atgttatccc agagtgtcgg tttcgctacg
     1261 aatgacgacg actggagcag taacgactca ttcttcaagg gaaaacgacc tggtttggat
     1321 cctaattccc aaagcaccaa tatttttgga tcgggagcga gttcgggaag ttcgaacaag
     1381 agtcgcggtc gagaagaagg taatgaaaac gaaggaaccg tcacgggttc agccaccgtt
     1441 agaataataa ggcctccgtc agaggcgggc ggctcggaac caaagctcga gaaaacacca
     1501 acgcttgata atgaggccgt tattcgactt gtggaagccg ggttctctga aggcaccata
     1561 attaagcgga ttgaaggctc tccggctgat tttgatctct caccatcaaa attagatgac
     1621 cttcataagc gtcgtgtaac ggatgccatc attactgcta tgactctcgc aatgaacgaa
     1681 aaaggttagt cgacctattt aatcttaagt tcaaatcaaa acttcagcac cagacattaa
     1741 aaaaggtgga ccctgtcccc gctcaactcc caggctgtga ctctgatcgt ttagttggac
     1801 aactcaatct gagttcagag tttggtcgag ttgagcgggg acagggtcta accttatttg
     1861 agagagaaaa ttggccctca gggtctggcc aaaatgttac agaggaccct cttaacctag
     1921 tccccgtatc attgagacac atctcggcac gggcctcaaa cgcgcatcga tcttcctaaa
     1981 tattgaaata gttgaagttg agcggtttgc ttcccaacgg cacacccgtt gccatcatga
     2041 aagtcgcttt ggcgagcagt gatctgcctg gcattaataa taaagacgca attgtagaag
     2101 attcgagacg gaggaaagat gcgaccaatc aagcacgcgg aacgaggatt gaccttttta
     2161 gcgggaagga ctttcaatct aattcaatcg gtaaacaaat tcaggccgaa cccctctttt
     2221 acaccaaagt ggtctgaaaa accccttttg aaatcctggc agaagagtaa gccaacgctt
     2281 ggctggccgc gcaccacaga ttctctttgc ccgaactgcg taatcgaagc gagagaagcg
     2341 atcttgtcgg gtaagaagga cgtcagtgtc cttatcaacg agaaggttgg agaaatcaag
     2401 gcacagatta ttgagcgaga cggtgagatc tggatgatca aagactgtcc gcagcacggt
     2461 cacttcgaag atatgatggc gatcgattca aagttcctga cgcacatcga gaagatgttt
     2521 ccaggtcgtg acatcgacgc tcacaacgac gaaaaacttc ataaccatgg atccagtacg
     2581 gtcaagtatg gccgcggcgc agtgctcaca gtagacctta ccaaccgttg caacatgatg
     2641 tgtgacccat gcttcatgga tgcaaaccag gtaggtttcg ttcacgagct cagcatggac
     2701 gagatcaaag aaattcttga caacgcgatc accatcaagc cgagacgtca gatgtcggtc
     2761 cagttttcag gtggtgaacc gtccatctcc ccgcacttcc ttgaagcaat caagtattcg
     2821 cgtaaggttg gttacaacag cgttcaggct gccacgaacg gaatcgaatt tgccaagagc
     2881 aaagagtttt gccgtgaggc cgctgaagct ggattgcgtt atgtctacct gcagtttgat
     2941 ggcattggta acgacgctaa cagccatcgc caggtaggca acctgttcga cgtgaagatg
     3001 cgcgccatca acaacctgca tgaagccggt gttgagattg tgttagtgac cacgctcgtc
     3061 aacggaatca acaacgatca ggtgggctca atcatccgct tcgctctgga taatcctaag
     3121 aagattgcgt ttctgtcgtt ccagcctgtt tcgttcactg gtcgtgacga agagatcacc
     3181 gagcatcgcc gtttacagca acgctacact ctttcacact tggcccatga cgttaagaat
     3241 caggttggca tcacagagcc aacgcgcgac tggttcccgc tttctctgat gggagctttc
     3301 gccgattttg ccgatctcgt acacgggcca gaagctgagt ggggacaggt ttcgtgcggt
     3361 tgccatccca actgcggcgt tggtactgcg gtgatgatcg ataaagaaaa taaggaaatg
     3421 agaccggtcc cagagtttct taacattccg ggtctggtca aggacatgca gaagatcacc
     3481 gatgcggcgc gcggcaagtg gatgtcgaac gtcatgatgg gacttacgct gttgaagcac
     3541 tacaatccgt ataagtcgcc ttcacagttt acgctttacg agctcttcaa gaagttcgac
     3601 aagtcgtttg gtttaaccgg caaggattac ggtagggtag gtggcgaccg cagcaagaac
     3661 gatatcgaaa agagacgctc cgatcgctgg aatttcctat ttatcgccgg aatgtggttc
     3721 caggatctgt tcaactacga tttcaggcgc actgaaatgt gcatcattcc ctacggaacg
     3781 caggaaggtg agatctcgtt ctgcgcgtac aacaccggca tcggttggcg caatattatt
     3841 gagaacatgc accagaacgc gactgtggcg aaatggtatc aggaacatgg acggcacgag
     3901 atatttgctg gtggcaaaga tgtgcaactc gcagataagg cgcatggcat gattttaaat
     3961 gaggttgacc tggctcgacc gaataagccg cagatggaag gtcccaagac tgcgcacgaa
     4021 gaagccgtca tgatgcggaa gatgtatcag gagatggttc tgaacaagca gctcgacgtg
     4081 aaacaagccg acaaacccgt tcaaatcaag gggttgggtc gtaagaaaga ggaagaggtc
     4141 gaagccgtcc tcgtttaggg tcaagccacc atcctcgagc cccgcgagca gccacctgag
     4201 cgcggggttc gattgtgtaa atcgactgat tgtcgctgcg aacttgaccc aatgcattga
     4261 tggcgtgtta aatatctgtc ccgaaagcga actgacgtaa aggaaagcac agaaagaatg
     4321 gcaatagtta ctgttaatca aggtgagtcg attgaaagcg cattaaggcg tttcaaacgc
     4381 aaggtaatga ccgaagaaat tattaaggac gccaagaagc acgcattctt tattccgccg
     4441 ggccagaagg cgaagttgaa atccgcgctt gcacgtaaac ggaataagaa aaaaggccga
     4501 atgcgtgtcc cgcgtgacta gttagtgctc ccctgttaag cggcgctggc cgcactctta
     4561 tctcaccagc cgtctttaac tgaaggccga taagtggacg ctcctgagtg agcggaccat
     4621 cgaaagtgga gtgtgccaac catcactccg tgagatgata gacgcagatg acacgaattc
     4681 tggttacaaa cgacgacggt attcactctg acggcctgat tcaactggaa caggccttga
     4741 aagagttagg cgatgtatat gtagttgcgc ccgcctcgga aatgagtggt gcgtctcata
     4801 gtctcacact ggcccgaccc ttgaggataa gacagttgga ctcccgtcat tggtcaatcg
     4861 atggtactcc gactgattgt gtgaccctcg ctttaaatcg aatcctttcc gagaacgaac
     4921 gtccggatat ttgcgtgtct ggaattaatc acggcggtaa cctgggcgat gacgcaacgt
     4981 attcgggtac cgtagccggt gcactggaag cgactatcct tggcgttccc ggaattgctc
     5041 tcagtctggt ggcacgggag aacttcgact ttaccgaagc cgcagccttt gcagttgtgg
     5101 cagttcgtaa agttctcgaa gggcatttgc cagaaggcac tctcctgaac atcaatattc
     5161 cccccggtga aatcaaaggt gttaaagtgg cgcggcaggg aatcaaaaat gcccgacccg
     5221 ttatcaccga acatattgac ccgcgaggta aaccgtactt ctggatcggc gaagagtatt
     5281 tcaataccaa ttcggtcgaa ggaaccgact atcatgcggt ccaggccggt tatatctctg
     5341 ttacaccatt gcgcagtgac atgaccgatc accaggcgct tacagccctt gagacctgga
     5401 actatctaaa agattcagaa ctattgcaaa agagttaatc actgaattgc acacatcgca
     5461 gcccttaaac cgacaa
//
LOCUS       DASSFP010000192         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1083400, whole genome shotgun sequence.
ACCESSION   DASSFP010000192 DASSFP010000000
VERSION     DASSFP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083400"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..826)
                     /locus_tag="VFH91_04160"
     CDS             complement(41..826)
                     /locus_tag="VFH91_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862210.1"
                     /translation="MKPSPLLRKFFFFSLSVSSVVALALTGGTIKNSAADFTGREILS
                     VSRIAHGGADYTGLQNVTVNAGGFVNAAAFGAIGANPLGAAAEVKLKITDYQDKNMRR
                     RLDIAPTATLPGPTYLVFTGSTGGGMIFGNEFRVSEAAASRHWGMMGFDTLNRAIDGQ
                     LVTARQKDEGNDYVVEVKFNPQDTVRYWINMQTFLIDKITTRYNSQVLIEENRSDYRK
                     ADCMMLPFHVVTRLQGQRFADLQIDNYDLKTRVPEARFTISAQ"
     gene            complement(906..4037)
                     /locus_tag="VFH91_04165"
     CDS             complement(906..4037)
                     /locus_tag="VFH91_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6862211.1"
                     /translation="MAQITLAAEIKGVRQPEVVYPGPSIDVGRNPNTCKLVYDQKEWP
                     SVSRQHAQFLLHEGRWFISDAGSTYGTYVNGQQIKTPTELHVGSHVQFGQNGPVVVVT
                     ALATGSSPLTAGDTIIDSEQTIGQFSQSNLQPTRPPKPDTTGPASVELSGTGTADLRL
                     IKLNTSTPARFGREPELEGPINAAAAMVSRRHAEIRWQDGQYVIVDLGSFNGTLLNGH
                     RIAKPEALRHNDRIELGPGGPLIRFLAPNSSPIPSESSAPVAQAFHQPANSGKLQTMV
                     LGSGSPLQITGGAEAGVFVQKSFANNQRLVVGRGEECDIRLDGLLISNRHAAVALTAS
                     GLVLEDLNSTNGTYVNGRRINGRAPIQTQDVIQIGPFVLRADPQRGVAIFDTRAKTRI
                     DVFQITKDVSNRSGRGKVRLLDEVSLSIQPNEFVGLLGPSGAGKSTLMDSLNGMRPAS
                     SGQVLVNNLDLYHHLESIKQSIGYVPQDDIIHRELTVYRTLYYVARLRLSRDISVHEI
                     NQIVNEVMDVTGLSERRDVTIAQLSGGQRKRVSIAVELITKPSVIFLDEPTSGLDPAT
                     EEKVMKLFRQIAESGRTVILTTHAMENVKLFDKIVVLMRGKLVFYGAPEEALAHVGAS
                     SFKELYDKLEAPIYERLKQSNGNVSREQISEEVANEWKRRFTTTEQYKRNVANPLTEV
                     SSKPRQAAPAKRRTTVGDSVRQWGTLSRRYMGVLTRDKFNLLILFGQAPIIAFLTYIV
                     VGANSPRDFPFFVLALVALWFGTSLAAREIIRERAVYNRERMVNLRLLPYVGSKVFVL
                     TLIVSLQCLILFTTLKLLDLTNLMSLPGRLGGIPQLIIMILTGMVGIGLGLFVSAVVK
                     TSEMATSLVPLILIPQILFCGLVGVPVGMGRVIGVVMPATWSFDEMKRLSGLDVLRGK
                     DEKAQASVNNDGRGLYKKIERENDQNIADAQTKIRNYKSDAEKSLKDFEKDMDQYQKD
                     LMARGSTTKPTAPTLSEVPTIQKASQVPEDLSTFVDFLHPWGSHLVDSMALLTMLFVL
                     LSATGIALRMQDI"
BASE COUNT         1014 a         1063 c         1096 g         1056 t
ORIGIN      
        1 aatcaggcta gccatcgcag gacccaaaat tttcccgtca ctattgagcg ctgattgtga
       61 agcgtgcttc gggaactcgg gttttcagat cgtagttgtc aatctgaaga tccgcgaagc
      121 gttggccctg tagtctagta acgacatgga acggcagcat catgcagtct gctttgcgat
      181 aatcgctccg gttctcctcg attagtacct gactgttgta tcgcgtcgtg atcttatcaa
      241 tcaggaaagt ttgcatgttg atccaatatc gaaccgtgtc ctgaggattg aacttgacct
      301 cgactacata atcgttacct tcatcctttt gacgagcagt aacgagttga ccgtcgatcg
      361 cccggtttag ggtgtcaaat cccatcattc cccaatgacg cgaagcagca gcctcgctaa
      421 ctctaaattc gttgccgaaa atcatgccgc cgccggtcga accggtgaac acgagataag
      481 ttggtccggg tagtgtcgcc gtcggcgcga tgtctagacg ccggcgcatg ttcttgtcct
      541 ggtaatcagt gatctttagt tttacttcgg ctgcggctcc aagcggattt gctcctatgg
      601 cgccaaaagc ggcggcgttg acaaaaccac cggcattcac cgtgacgttt tgtagacccg
      661 tgtagtcagc gccaccgtgg gcaattctgg aaacgctgag aatttcgcgt ccagtaaaat
      721 ctgcggccga gtttttgatg gttccacccg tcagagccaa agcaaccacc gacgaaacac
      781 ttaaagagaa gaagaaaaac ttcctgagta agggacttgg tttcatgttt gagcctccaa
      841 aagttttcaa actaaagact gtgattggaa attaagcgcc ggccttgacc gcgcatgcac
      901 acctgctaaa tgtcttgcat gcgtaaggcg attcccgtcg cagagagtaa gacgaacaac
      961 atcgttaaga gcgccatcga gtcgaccaga tggcttcccc atggatgaag aaagtcgaca
     1021 aatgtgctca ggtcttctgg tacctgagat gccttctgaa ttgttggaac ttcagaaagg
     1081 gtaggagctg tgggtttagt ggtggaacca cgtgccatca gatccttctg atactggtcc
     1141 atgtcttttt cgaaatcctt taaacttttt tccgcgtcac tcttgtagtt gcgaatcttt
     1201 gtttgggcgt cggcgatatt ctggtcgttc tcacgctcaa tctttttgta cagtccgcgt
     1261 ccatcgttgt tgactgaggc ttgggccttc tcgtccttac cgcgcaacac gtcgaggcca
     1321 gacaagcgct tcatctcgtc gaacgaccag gtggcaggca tcacgacacc aatcactcga
     1381 cccatcccga ccggaacgcc tactaatccg caaaagagga tctgtggtat caggatcaac
     1441 gggacaaggc ttgtcgccat ctcagacgtc ttaaccacag ccgaaacaaa tagtccgaga
     1501 ccaataccca ccattccggt caggatcatg attattaact gtggtatacc gccaagcctt
     1561 cccggcaaac tcatcaggtt cgtgaggtcc aggagcttca aggtcgtaaa cagaatcaaa
     1621 cactggaggc tgactataag tgtcagtaca aagactttcg aacctacgta agggagtaga
     1681 cgcagattca ccatgcgttc tcggttatat acagcacgct cgcgaatgat ctcccgcgca
     1741 gccaacgatg taccaaacca aagagctacc agtgcaagta cgaagaaagg gaagtcgcgt
     1801 ggcgaatttg ctccgacaac tatgtaggtc aaaaatgcga tgattggagc ctgaccaaag
     1861 agaatcaaaa ggttgaactt gtcgcgcgtt aacactccca tgtagcgccg ggaaagtgtg
     1921 ccccactggc gcaccgaatc gccaacggtg gttctccgtt tggccggtgc ggcttgccga
     1981 ggtttacttg aaacttcagt taaaggattc gcgacgtttc gtttgtactg ttcagtcgtg
     2041 gtgaagcgac gcttccattc atttgcaact tcctcggaga tctgctcgcg tgaaacgttg
     2101 ccgttagact gtttcaaccg ttcataaatc ggcgcctcga gtttgtcgta cagctctttg
     2161 aagcttgaag cacccacgtg ggccagcgcc tcttcaggag ccccgtagaa aacaagcttg
     2221 ccgcgcatca acacgacaat cttgtcaaac aacttcacgt tctccatggc gtgagtcgta
     2281 aggatgactg ttcgaccgga ttcggcgatc tggcgaaaca gtttcatcac cttttcttct
     2341 gttgccggat cgagacctga cgtgggttca tccagaaaga taacagaagg ctttgtgatg
     2401 agttctactg cgatcgaaac acgcttgcgt tgaccacctg aaagctgagc gatggttaca
     2461 tctcgtcttt ccgacagtcc ggtaacatcc attacttcgt tgacgatctg attgatctcg
     2521 tgaactgaaa tatcacgtga caatcgcaat cgtgcgacgt agtaaagcgt tcgatagaca
     2581 gttagttctc gatggatgat gtcgtcctgg ggaacgtatc caatcgattg cttgattgat
     2641 tcgagatgat gataaaggtc gaggttattg acgagtacct gaccgctcga cgctggacgc
     2701 attccattta acgagtccat cagcgtcgat ttgccggcgc cggagggtcc gagaagtccg
     2761 acgaattcgt ttggctgtat gctcagactt acctcgtcca acaaccgcac ttttccgcga
     2821 cccgagcgat ttgatacgtc ctttgtaatc tgaaaaacgt cgatgcgcgt tttagctcgc
     2881 gtgtcgaaga ttgcgacacc acgctgcgga tcagcccgca gtacaaatgg tccaatctga
     2941 atgacgtcct gagtttgaat cggagcgcga ccattaatgc gacgcccatt aacataggtg
     3001 ccatttgtgg agttgagatc ttccaacact aagcctgacg cggttaaggc gaccgcagca
     3061 tgtcgattag aaatcagtag tccgtctaat cgaatgtcgc actcttcgcc gcgcccgact
     3121 acgagtcgct ggttgttcgc gaatgacttt tggacgaaga ctcctgcttc agctccgccg
     3181 gtaatttgta gtgggctgcc agagcccaac accattgttt gcagttttcc ggaattcgca
     3241 ggttgatgga atgcctgtgc gacaggagct gaactctcag agggaatcgg cgaagagtta
     3301 ggagccagga aacggatcag aggaccgccg gggccaagtt caatccgatc gttgtgtcga
     3361 agtgcctcgg gcttggcgat tcggtgaccg ttcagcaatg tgccattaaa gctacccaga
     3421 tcgacgatga catactggcc atcctgccaa cggatctcag catgtcggcg cgacaccatc
     3481 gcggcagctg cgttgatcgg tccttcgagc tcgggctctc gtccaaaccg tgcgggagtg
     3541 gaagtattta gtttgatgag tcgaagatcg gctgtgccgg ttcctgacag ctcaacactc
     3601 gcaggtccgg tggtatcggg cttcggcgga cgcgttggtt ggagattgga ctgagagaat
     3661 tggccgatag tttgttctga atcgatgatt gtgtcgccgg ccgtgagagg acttgagcca
     3721 gttgccaggg ccgtgacaac tacgacagga ccgttttgtc caaactggac atgcgaaccg
     3781 acgtggagtt ctgttggcgt cttaatctgt tgtccattga cgtaagtacc gtaagtggaa
     3841 ccggcatcag agatgaacca tcgaccttca tgaagaagaa actgagcgtg ttgcctcgaa
     3901 actgagggcc actccttttg atcgtagaca agtttgcaag tgttgggatt acgcccaaca
     3961 tctatggacg gacctggata aacaacttca ggttgtctga ctccctttat ttccgctgct
     4021 agggtaattt gagccacgtt ttaagacgtt cgcaaactgg ggtagcgtcc ggtgtctgat
     4081 gagctttaaa agtcgctcgc gaagaagaca gtttttaact catgctgagc cgcttcgcaa
     4141 tgtttctttt cggaaatgag ataaaagaaa actcaggtcc ggtgcggcaa tgacctttct
     4201 cacttaggaa acgcgcaatt tcaggtcat
//
LOCUS       DASSFP010000193        10913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1535481, whole genome shotgun sequence.
ACCESSION   DASSFP010000193 DASSFP010000000
VERSION     DASSFP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535481"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1371
                     /locus_tag="VFH91_04170"
     CDS             352..1371
                     /locus_tag="VFH91_04170"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET6862212.1"
                     /translation="MAKRALITGITGQDGSYLAEYLLGLGYEVHGLVRRVALEAPEHR
                     FGRISRILDRVTLHPASLESYPSIFHVFSRNEFDECYHLAAQSFVAEGFADGFSTMNM
                     NINGTHYVLAALRELRPGCRFYFAGSSEMFGNADEVPQKEGTAFQPRSPYGISKVAGF
                     HLTRNYREAYGLFTASGILFNHESPRRGFEFVTRKTTSAVAQIKLGLASELRIGNLEA
                     KRDWGHAIDYVRAMHLMLQQPEPDDFVIATGQIRSVRELCDLAFSEVGLDYRDYVKVD
                     ERYYRPAEVDVLVGDATKARQVLGWSPTYTFEDMIKEMVHEDLTSLQQRSEKPANERI
                     SLSLT"
     gene            1619..4429
                     /locus_tag="VFH91_04175"
     CDS             1619..4429
                     /locus_tag="VFH91_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HET6862213.1"
                     /translation="MHYRPINRRLTRQIVSLAVTLFLTICTLPSSPSSAISLNPSKPS
                     PQGQGRIQEPNVPVIAPGSAYRQTNLISDSPGLAPVLDPLLVNPWGVTVLGTSPFWVS
                     NNGTSTTQLIVGDVGGAPVRLNAAPQTINIPGGLPTGLVGNSTTDFAITPPGGGTPAA
                     SVFIFSSITGNIIGWNGAQGATAKNAVSMPGHVWTGLAIGSNGGGNRLYAADFANNHI
                     DVFNQTFAATTVSGGFVDLTIPAGFAPYNIQNLGGSLYVTYAKVGVDGRAEDGVGKGF
                     VRKFNTDGVRDLTFGINNGELNAPWGLVIAPASFGIFGSALLVGNFGEGNPSIHAYNP
                     SSGAFLGTLQDESGNGIVIDELWGLIFGNGGNGGDPNTLYFNAGIGEEEHGLFGSLKP
                     TASTATSLIQLATDDSAISENSGHIDITVTRAGNVSGTATVNFATFDESQVGHASQKS
                     DYEIAVGKLTFNAGETSKTFRILLVDDKFVEGNETVDIVLSNPTGTGVGIGSPNRASL
                     TIVDDDVAPPVTNPVDDATFFVRQQYLDFLNREPDPAGLAFWVNEITSCGGNAQCVAV
                     KRVNVSAAFFLATEFQQTGYLAYLTHRVAFGPSAFGSPVPVLYGNFQRDTQALQKDFV
                     FGAPNGDAQLEANKVAFFNEFVTRPEFVSKYPSTLTDAQYVDALLATANLTPAEVRLF
                     VVNLTNAQEVPPTVPTNTNGSARPASFGTARFQFNQAQTAMTFTSTINNIDVTTSQTA
                     DTNDNLIAAHIHAGPTVVPGVNGPVVWGFFGNPFNDNNPNDQVFSPFSSGVGGTFSGK
                     WDAPEGNGTTLATQLPNLRAGHAYINFHTNQFGGGEIRGNFPAQNAFRDALVAGLGNA
                     SETRVTVLRKIAESAEMQSRELNAAFVALEYFGYLRRDPDTAGFNFWFNKLSAFNGNF
                     VDAEMVKAFLTSSEYRQRFGPA"
     gene            4669..5973
                     /locus_tag="VFH91_04180"
     CDS             4669..5973
                     /locus_tag="VFH91_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862214.1"
                     /translation="MSVALRVEQVSKQYRIYERPGDRLKESLTRGRLRRHKEFWALQN
                     VSFEIEAGTTVGIIGQNGCGKSTLLQIIAGTLAPTHGDVWHEGRIAALLELGAGFDPE
                     FTGVENAYMNASLMGFSRRETEAIFPRIERFAEIGQFIDQPVKTYSSGMYVRLAFAIA
                     ASVEPDILIIDEALAVGDAIFQHRCLQRIKELRDRGTTVLFVSHDTAAVRALCDRAIL
                     LSSGQMVGDGKPADVLNDYQRIVMERQDAYEASSTTNSFYTASGEPIAPLHYTYRHGD
                     GSAEIISAELCDASGRRTEIVGTGDNIYVQVTVRFSHDVDDPVIGFLIRNRHGIHAYG
                     TNTREQDLKVGKAGEGRTLSIRFAFNCWLGVDDYSVSFAVHSFEGHAYDWVDGTLFFK
                     VTSSRVTEGVANLNAVAQVVHDGNRLGSSIHQAEPSLTAPGA"
     gene            5976..6755
                     /locus_tag="VFH91_04185"
     CDS             5976..6755
                     /locus_tag="VFH91_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862215.1"
                     /translation="MVDQEVEEILKQIRDSVRAEEGRTKAFSVQTNNGASDLSGPPSS
                     DQVTHALQSYPNLSTLNRAWDQLPPVMSNRRGAIASAELWLKQILKRGSRWFTWEQVN
                     FNSAVHHTFREVIETLATYEQQLSRLNKQLSDTSDRLQKQQRRFDEFFEPYKGELRQE
                     LNAIRTKVAALEGELDVWQHKRQDEGKELKARVNESLIEIRAATERILDEQRVCFKQM
                     TLETGETSARDERERRALDQRITKLEGARESSSKATGGSDS"
     gene            6752..8080
                     /locus_tag="VFH91_04190"
     CDS             6752..8080
                     /locus_tag="VFH91_04190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6862216.1"
                     /translation="MSSGPEESWYKRSGIDDLVEFYRTKFVSDEVLDSRYFKALDRFD
                     MRFARTMWVFDNVRSGSTVLDLGCGAGMLALLKKKDVTLAGIDLSSECALASKRNGYD
                     FTFTGELTNLPFEDHSFDYVVSLDVFGHIEFSQKDQVIREIKRVLRPDGVTLHGIEST
                     NRSLRPAMTEMSAEELHRFIAVDGHVGLEEEQEHAERFQRFFSNVAFEPRYALCLTSE
                     EFLKQADHYGLPFDKDLLDYLRGLSFKERRAFDIAMGYVFGRISELNIKLPKAGLYVL
                     VKASDSELGPFYNEHRDRSSLLPLAVQDLPLSLDRRSDVVFDDGWYEPNLLPPVARWM
                     AQNARLRFKVESLSSVSFDLTTHIPDLQAKPIELELLVNGRQLSAISLYRYGWLRWQI
                     QIPQELGTAGDFEIELRSNRTWQPRPSGKAEDGRDDRELSIAVCNLEITP"
     gene            8141..9193
                     /locus_tag="VFH91_04195"
     CDS             8141..9193
                     /locus_tag="VFH91_04195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6862217.1"
                     /translation="MIRNILICTTQVPFTTGGAELHVEGLRGALVEAGYHAEVVSLPF
                     KWYPPAEIMRSALAWRLLDLSESNGKKIDLVIGMKFPAYLARHDSKVLWVMHQYRSAY
                     NLWGTAFDDLSTYPEGTRIRQWIHRCDNQFIGAAKKVFANSKTVARRLRDFNNIDSEP
                     LYHPPPLSGKLNGGDQGDYIFYPSRLEPQKRQELLIEALRHTTTDARIVFAGGSANLQ
                     HYADIAKKAGVSDRVCLLGFVDEAKLIDLYSNALAVSYLPFDEDYGYVSLEGMLAAKP
                     LIVTKDGGGAAEFVEDQITGFVVDPEPQALAEAIDQIYTDRNQAGRLGERGREKFVSQ
                     NISWPNVVEKIISAAT"
     gene            9201..10913
                     /locus_tag="VFH91_04200"
     CDS             9201..10913
                     /locus_tag="VFH91_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6862218.1"
                     /translation="MLRRISFIHAPDRFLAETQQYGAMFMPVWAYTLAAYIEEPEKYD
                     LQLVDMRFDRVEDVGEADLFVFSGINQDYETIIATREKLHWRFPKATFIVGGPITWSL
                     NEAQEIAKLGMFDKIFIGDGEEAFPKFLHQFSYRDTLPKIIKTTNRFDVTRARGFYRP
                     FLNETFQRYYGAVLEVSRGCPFLCEFCDIRVLPDNNRAHNFSVEHIVSELDAIASLGI
                     KQVLCAADNFIGDPRWAEELLDAIISWQRKTNSPVALYTWLTINLARHPTLLRKFREA
                     NFDMLFIGVESFSNNSLLETAKVQNSTVDMVDAIQEIQSYGLPVVAGLIFGFDSDTKN
                     AFDETIEGMSNAGLISGDPSLLTALPGTPLFRRMKLAGRLRNNKNSLGGYKYCTNIRY
                     LMPAEEIIEGYRTFVSRFCEGPYQYQRLSSFLNNLDRGNYIPLRSTGYGSLGKYLTMV
                     FKSPNATRMLLQRLWQIMSRPAVAWYVFRGLLLVIRKSRRHAKLFGIFQFWLFNWTNA
                     MLKYEGLSAKDFDIESVPKDFDRSLILPENYTDAAHEQIPQAKINAQQRITMTQLRRL
                     TVPQ"
BASE COUNT         2743 a         2742 c         2772 g         2656 t
ORIGIN      
        1 gggcagacag gatgagagcg cacatgaacc agaaagcttg cttcacgaag atcctccgaa
       61 ataaagacgc agatacgagc ggattagatg ccctcgggga gcgggagcag cgccgtactc
      121 aggtgaatta ttagtgccga cgtcggcgct taaagaacgg gcattttaag cacaacccgc
      181 tcagaccgca agcatcgaga aatttaggta gtgtcacctt cgtcaacccg tgacactgtt
      241 tcacaacgca acagattctg cacaacaatt tgttccacat gcgagcgcat atactttatg
      301 atgcagtcta tctcttaatc gaaattacgc accgaatgtc gaggaaacgt tgtggcaaag
      361 cgcgctctaa ttacaggtat taccggccag gacggaagtt acctggccga atatcttttg
      421 ggactgggtt atgaggtcca tggactagtc cgccgagttg cgctcgaggc tccagaacat
      481 cgcttcggac gaatctcacg cattctcgat cgcgtaactc ttcatcctgc cagtttggag
      541 agctatccca gcatttttca cgtgttcagt cggaatgagt tcgacgaatg ctatcacctc
      601 gcggctcaaa gtttcgtcgc agagggtttt gctgatggct tttcgacaat gaacatgaac
      661 atcaacggaa ctcattatgt actcgctgca ttgcgtgaat tgcgaccagg ctgccgcttc
      721 tactttgcag gttccagtga gatgttcggc aacgctgatg aggtgccaca gaaggaaggc
      781 accgccttcc aacctcgcag cccttatgga ataagcaagg tcgctggatt tcacctaact
      841 cgaaactatc gcgaagctta tggtctattt accgcgagcg gaattctctt taaccacgag
      901 agtccacgcc gagggtttga gtttgtgaca cgaaagacaa ccagtgcggt ggctcaaatc
      961 aagctgggac tcgcgtcgga actaagaatt ggaaatttgg aagcaaaacg agactgggga
     1021 catgcaatag attacgtgcg agcaatgcat ctgatgctcc aacaacccga gccggatgat
     1081 ttcgtcatag ctacgggtca aattcgtagc gtgcgcgagt tatgtgatct ggcgttcagc
     1141 gaggttggcc ttgattatcg agactacgta aaagtggatg agcgatatta tcgacctgct
     1201 gaagtggacg tgcttgttgg cgatgcaacg aaagcgaggc aggtgctcgg gtggagtccc
     1261 acctatacgt tcgaagacat gatcaaggaa atggttcacg aagacctaac aagtcttcaa
     1321 cagagatccg aaaaaccggc caacgaacgc atttctttgt ccttaacctg accagtactt
     1381 atcggacggt ggtcccacaa atttcttgac agtaaataac gagggactat agagtttcgc
     1441 gcgtcctctc agttcaaacg actcagattt caagtagcgg tcaaagccgc ttctgaatga
     1501 cgacacaaac tccccaatcg tcacccgtcc cgattagtgc ggagtgtctt ttcctgccca
     1561 ccgtgccggg accagcagca agatcaaatg aagtgtagag cttactagga ggatcccaat
     1621 gcactatcgg cccattaacc gacggctaac gcgtcagatc gtaagtttag ccgtcacctt
     1681 gttcctaaca atctgtacct tgccatcgag cccaagttca gcaatctcgc ttaacccatc
     1741 caaaccctcg cctcaagggc agggtcggat tcaagaaccc aacgtcccgg taatcgctcc
     1801 gggctcggct tatcgacaaa caaatctcat ttcagactcg ccgggattgg ccccggtact
     1861 cgacccttta cttgtaaatc catggggtgt gaccgtcctt ggtaccagtc ctttctgggt
     1921 ttccaataat gggaccagca caacgcagct aattgttggg gacgtcggtg gagcacctgt
     1981 caggttgaat gccgctccgc aaaccataaa cattcctggc ggtcttccga caggacttgt
     2041 cggcaactcc acgacagatt ttgcgataac acctccaggt gggggaacac ctgcagcatc
     2101 ggtgttcatc ttcagctcaa tcaccggcaa catcatcgga tggaatggag ctcaaggtgc
     2161 caccgcgaag aacgctgtaa gcatgcccgg tcatgtttgg acaggtctcg cgattggttc
     2221 aaacgggggt ggtaatcgtc tttacgctgc cgattttgcc aataaccaca ttgatgtctt
     2281 caatcaaact ttcgccgcca cgacagtaag tggcggtttt gtggatctca ccattccggc
     2341 aggatttgct ccttacaata tccagaacct gggcggttca ctttatgtga cttatgccaa
     2401 ggtcggcgtg gatgggaggg cagaagatgg tgttggcaaa ggttttgttc gcaaattcaa
     2461 caccgatggt gtccgcgatc tgacttttgg catcaacaat ggagaactga atgcgccatg
     2521 gggacttgtg atcgcgcctg cgagcttcgg aatttttggt agtgcactgc tagtcggcaa
     2581 ctttggtgag ggcaatccaa gtattcacgc ctacaacccc tcctccggcg cttttcttgg
     2641 gactcttcag gatgaatccg ggaatgggat cgtaatcgac gaactctggg gattgatttt
     2701 tggtaatggc ggaaacggag gcgatccaaa tacactctac ttcaacgctg gtatcggaga
     2761 agaagaacat ggacttttcg gctcgctgaa accaaccgcg tcaaccgcca cgtcactcat
     2821 tcaactggcg accgacgact ctgccatatc agaaaatagt ggccatatcg acatcacagt
     2881 cactcgcgct ggtaacgttt ccggtacagc aacggtcaac ttcgcaacct tcgatgagtc
     2941 tcaggtcggt cacgccagtc agaagagcga ttatgagata gctgtcggaa agctaacctt
     3001 caatgccggc gagaccagca agacattccg cattctcctg gttgatgaca agtttgtaga
     3061 gggcaacgaa accgtcgata ttgttctaag taatccgact ggaacgggcg taggaattgg
     3121 tagtccgaat cgggcgtcgc tcacgattgt agatgacgac gtagcgcctc ctgtcactaa
     3181 cccagtcgac gacgctactt tcttcgttcg ccaacaatac ctcgactttc tgaatcgcga
     3241 acctgatccc gccggactcg cgttctgggt aaacgaaata acctcgtgcg gtggcaatgc
     3301 ccagtgcgtc gcggtaaaac gagttaacgt cagtgctgca ttcttcctgg caacagaatt
     3361 ccagcaaact ggttatctcg cgtacctgac tcatcgtgtg gcctttggtc caagtgcttt
     3421 cggatcgcct gtgccagtcc tttacggcaa tttccaacgt gacacgcagg cgctccagaa
     3481 agactttgta tttggagcac caaacggaga cgctcaactg gaagcaaata aggtagcgtt
     3541 cttcaatgag ttcgttacgc gaccggagtt cgtttccaag tatccgagca cgttgacaga
     3601 cgcgcaatac gttgacgcct tgcttgcaac tgcgaatttg acacctgccg aagtgcggct
     3661 gttcgtagtg aatttgacga acgcgcagga ggtaccacct actgtgccga caaacactaa
     3721 tggttcggca aggccagcgt ccttcggcac agctcgtttc caatttaacc aagcgcaaac
     3781 ggcgatgacg tttacgtcga ccattaacaa tatcgacgta acgacatccc aaacggcgga
     3841 tacgaacgac aatctcatcg ccgcccacat tcatgccggt ccaaccgttg tgccaggtgt
     3901 caacggaccc gtcgtctggg gattttttgg caatcccttc aacgacaaca atccgaacga
     3961 ccaggtattc tcaccatttt cgtccggcgt tggtggcact ttcagtggca agtgggacgc
     4021 acctgaaggt aatggcacaa cgttggcaac acagttacct aacctcagag ccggtcacgc
     4081 atacatcaac ttccatacga accagttcgg gggcggtgaa atccgtggca acttcccggc
     4141 gcaaaatgca tttcgcgatg cacttgtcgc cggccttggt aatgcatcag aaacgcgtgt
     4201 aacggttctt cgtaaaattg ctgaatcagc agagatgcaa agtcgtgaac taaacgctgc
     4261 gttcgtagcc ctggaatact ttggttactt acgacgcgat cctgacacgg ctggcttcaa
     4321 cttctggttt aataagctga gtgctttcaa cggcaacttc gtggatgcag agatggtcaa
     4381 agcattcctg acgtcttcgg agtacaggca gcgattcggc ccagcctaaa gacaattgag
     4441 tcttgtaaac tccatcaccc cggccactct caacggtggc cgggttcttt tctgcggggg
     4501 tcggagtcag aaaagaacaa ttgagaatta tcaattgata attctcaatt attaactacc
     4561 gaacctcgac cgctcgattg ctatctgccg acctggaccc caactcatga cccctggccg
     4621 ctggcccctt tctggctact tcgaacatct gttaaatttg cacaccaaat gagcgtcgca
     4681 ctccgcgtcg aacaggtatc taaacagtac cgaatctatg aacgcccggg agaccgtttg
     4741 aaagagtctc ttacacgggg acgtctgaga cgtcataaag agttctgggc attgcagaac
     4801 gttagcttcg aaatcgaagc gggaacgaca gtcggaatta tcggacagaa tggttgtgga
     4861 aagtctacgc tgctgcaaat catagcgggc acgcttgcgc ctactcatgg agacgtgtgg
     4921 cacgaaggtc ggatagcggc attacttgaa ctcggtgccg gatttgatcc cgaattcacc
     4981 ggggtcgaga acgcctacat gaatgcatcg ctgatgggct tttcacgtcg cgaaacggaa
     5041 gctatctttc cgcgaatcga acgcttcgct gagattggac aattcataga ccagccagtt
     5101 aagacttatt cgagtggtat gtatgtccgt cttgcattcg caattgcggc gagtgttgag
     5161 ccggacattt tgatcatcga tgaagctctt gcagttggtg acgcgatctt tcaacatcga
     5221 tgcctgcaaa gaatcaaaga gctgcgcgat cgcgggacca ccgtactctt cgtctcccat
     5281 gacactgcag cagtcagggc tctctgcgat cgagcaattc tgctaagctc gggacaaatg
     5341 gtcggtgacg gaaagccagc cgacgtcctt aatgactacc agcgcattgt gatggaacgc
     5401 caggatgcgt acgaagcttc cagtacgaca aactcgtttt atacagcttc gggtgagcca
     5461 atagctcctt tgcattacac ctaccgtcac ggtgatggaa gtgcagaaat tatttcagca
     5521 gaactttgcg acgcgtccgg tcgtcgcacg gaaatcgtgg ggacgggaga caatatttat
     5581 gttcaggtta ccgttcggtt ctctcacgac gtcgatgatc cagtaatcgg attccttatt
     5641 cgtaatcgac acggaattca cgcgtacggc accaatacac gagaacagga cttgaaggtc
     5701 ggcaaggctg gcgagggcag aacgctcagc attcggttcg cattcaactg ctggctcgga
     5761 gttgacgact actccgtgtc tttcgccgtc cacagtttcg agggtcacgc ttacgactgg
     5821 gttgacggaa cgcttttctt caaggttacg agctcaaggg ttacggaagg tgttgccaat
     5881 ctaaacgcgg ttgcccaggt tgttcatgac ggaaaccgtt tgggctcttc aattcatcaa
     5941 gccgagccaa gtctgactgc accgggagct tagttatggt tgatcaggaa gtcgaagaga
     6001 ttctgaaaca gataagagac agcgtccgcg ctgaagaggg gcgaaccaaa gcattctcgg
     6061 ttcagaccaa caatggcgcc tctgatctaa gcggtcctcc ttcgagcgac caagtaacgc
     6121 acgctcttca aagttaccca aacctttcca cgctgaatcg tgcctgggat caactgcctc
     6181 ctgtgatgag caatcggcga ggagcaatcg cttctgccga attgtggcta aagcaaattt
     6241 tgaaacgagg ctcgcgctgg tttacctggg aacaagtaaa ttttaattcc gcagtccacc
     6301 atacatttcg agaggttatt gaaacgctcg ctacttacga gcaacaactt tctcgcctca
     6361 acaagcagtt gagcgacacg agcgaccgac tgcaaaaaca acaacgtcgg tttgatgaat
     6421 tttttgagcc ctataaaggc gaactcaggc aagaactgaa tgcgatccga actaaagttg
     6481 ctgccctcga aggtgaattg gatgtttggc aacacaaaag acaagatgaa ggcaaggaac
     6541 ttaaggcgcg cgtaaatgag tcgttgatcg agatacgagc agctacagaa cgaatcctcg
     6601 atgaacaacg agtctgcttc aagcaaatga ctctcgaaac cggtgagact tcagcgcgtg
     6661 acgaacgcga acgacgagct ttggatcaac gaattacgaa gttagaaggt gctcgcgagt
     6721 cttcgtcaaa agcaacaggt gggagcgact catgagttcc ggccccgaag aaagctggta
     6781 caaacgatct ggcatagacg acctggtcga attttatcgg actaagtttg tttccgatga
     6841 ggttctcgac tcccgctact tcaaggccct cgatcgtttt gacatgagat ttgcacgtac
     6901 gatgtgggtc ttcgacaacg tacggtcggg ctctaccgtc cttgatctcg gttgcggagc
     6961 cgggatgctt gcccttttga agaagaaaga cgtaacgctt gcgggaatcg atctatcgag
     7021 cgaatgtgca ctagcctcaa aacgcaatgg ctacgacttc acgtttacgg gtgaattaac
     7081 caacctaccg tttgaggatc acagcttcga ctacgttgtt agtttggacg tgttcgggca
     7141 catcgagttc tcgcagaaag atcaagtcat cagagagatt aagcgcgtgc tgcggcccga
     7201 cggcgtgact ttgcacggga tcgaaagcac caatcgcagc cttcggccag cgatgactga
     7261 aatgagcgcg gaagagctac atcgttttat tgcggttgac gggcatgtcg gtctggaaga
     7321 agagcaggag catgccgaaa gattccaacg attctttagc aatgtggcat tcgagccacg
     7381 ctatgcctta tgtctgacaa gtgaagagtt cctgaagcaa gcagaccatt atggattgcc
     7441 atttgacaag gacctgctcg actacctgcg cggcttatcg tttaaggaac gacgggcttt
     7501 cgacatcgcc atgggttacg tctttggtcg gatctctgag ctcaatatca agttgccgaa
     7561 ggcgggactc tacgtcctgg ttaaagcgtc agacagtgag cttggtccgt tttacaacga
     7621 acatcgagat cgcagttccc tgttaccttt agcggttcag gacctacctc taagcctcga
     7681 tcgacgcagt gatgtggtat ttgacgacgg atggtacgag ccaaatttgt tgcctccggt
     7741 tgcacgctgg atggcccaaa acgccaggct tcgatttaag gtcgaatcac tcagcagtgt
     7801 ttcatttgat ctgacaactc acattcccga tcttcaggca aagccaatcg agcttgagct
     7861 gctcgtcaac ggacgacaac tctctgctat ctcgctttat cgttacggct ggttgcgttg
     7921 gcaaatccaa atccctcagg aactcggtac tgctggtgat ttcgaaatcg agttgagaag
     7981 taatcgtacc tggcagccaa gaccctctgg caaagctgaa gacggacggg acgatcgaga
     8041 gttatccatc gcagtttgca acctggaaat cactccttga gcgagacact taccatccgg
     8101 gtttagtctt tcagcgaact aacaaaattt cgtcgacgat atgatccgta acattctgat
     8161 ttgcaccacc caagttccat ttacgactgg aggcgccgaa ctccacgttg aaggcttgcg
     8221 cggtgcgctt gttgaggccg gatatcacgc tgaagtggtt tcacttccat tcaagtggta
     8281 tccgccggct gagattatgc gtagcgctct tgcctggcga cttctcgatc tgagtgaatc
     8341 gaatggcaag aagatcgatc tggtgatcgg catgaagttt ccagcctacc tggcccggca
     8401 tgactcaaaa gtgctatggg tcatgcatca ataccgctcc gcgtacaatc tttggggaac
     8461 agcttttgac gacctttcaa cctatcccga aggaactcgg atcaggcaat ggattcatcg
     8521 gtgtgacaat caatttatcg gcgccgcaaa aaaagtgttt gccaattcga agacggtcgc
     8581 acggcgctta cgcgacttca acaacatcga cagtgaaccg ctgtaccatc cgcctccttt
     8641 gtctggaaag ttgaacggag gcgatcaggg tgattatatt ttttatccga gccgtctcga
     8701 gccccagaag cgacaggaac tcttgatcga agccttgcgt cacacgacca cggacgcgag
     8761 gattgtattc gctggaggtt ctgcaaatct tcaacattac gccgatatag caaagaaagc
     8821 cggcgttagt gatagagtct gtttgctcgg gtttgtagat gaagcgaaac tgatcgattt
     8881 gtactcaaac gctttggccg tgtcctatct gccatttgat gaagattatg gctatgtatc
     8941 gcttgaagga atgcttgctg cgaaaccgtt aatagtcaca aaagatggtg gtggtgctgc
     9001 ggaatttgtg gaagaccaaa taacgggatt tgtggtcgat cctgaacctc aggcgctggc
     9061 tgaagcaatt gatcaaatct acaccgatcg taaccaggca gggcgtttag gcgaacgcgg
     9121 tcgagagaaa tttgtgagtc aaaacatctc atggccgaac gtggtggaaa agataatttc
     9181 tgctgccact tagtgtgatt atgctgcgac gcatttcttt cattcatgcg ccggatcgat
     9241 ttcttgccga gactcaacag tacggcgcga tgtttatgcc cgtctgggcc tacaccctcg
     9301 ccgcctacat cgaggagccg gaaaaatatg atttgcagct tgtcgatatg cgcttcgatc
     9361 gagttgagga tgtgggtgaa gctgatctat ttgtcttcag tggtatcaac caggattacg
     9421 agacaatcat tgcgacgcgt gaaaagctac attggcgttt tccgaaagcc acttttatcg
     9481 tcggtggtcc aattacctgg tcgttgaacg aagcgcaaga gatcgcgaag ctcggtatgt
     9541 tcgataagat ctttatcggc gatggcgaag aagcttttcc aaagttcctg catcaattct
     9601 cctaccgcga cacgttaccg aagatcatca agacaactaa ccgtttcgat gtgacccggg
     9661 cacgaggttt ctatcgaccc ttcctgaatg aaacttttca gcgctactac ggagcagtac
     9721 tggaggtctc gcggggctgt ccatttcttt gtgagttttg cgacattcgc gtcttgcccg
     9781 ataacaatcg agcacacaac ttttctgtcg aacatatcgt ttcggaactt gatgcgatcg
     9841 cctcgcttgg tattaaacag gtgctctgtg cggccgataa tttcatcggg gatcctcgat
     9901 gggccgaaga attactcgat gcaataatct cctggcagcg taaaactaat tcacctgtgg
     9961 cgctgtacac gtggctaacg atcaatctag ccagacatcc aactcttttg cgaaagttcc
    10021 gcgaagcgaa cttcgacatg ttgttcattg gagttgaaag ctttagcaac aattctcttc
    10081 tggaaacagc gaaagtacag aacagcactg tcgacatggt agatgcgatt caggaaattc
    10141 agtcgtacgg cttgccggtt gttgccggac tgatcttcgg gttcgactct gacactaaga
    10201 atgctttcga cgaaacaatt gaaggcatgt ccaacgccgg tttgatctcc ggtgatcctt
    10261 cattgctgac cgcgctacct ggtactcctc tctttcggcg catgaaactc gctggcaggc
    10321 ttcgcaataa caagaacagt ttgggtggct ataagtattg cacgaacatt cgttacctga
    10381 tgccggctga agagatcatc gagggctacc ggacgtttgt gagtcgcttt tgcgaaggtc
    10441 cttatcaata tcaacgattg agcagttttc taaataacct cgatcgcggt aattacattc
    10501 ccctgagatc aactgggtac ggcagccttg gcaaatatct tacgatggtc ttcaagagtc
    10561 cgaatgcaac tcgcatgttg ctgcaacgcc tttggcagat tatgtcgaga cctgcagtcg
    10621 cctggtacgt ttttcgtggt ctgttactgg taattcgcaa gtcacggcgc cacgcaaaac
    10681 tattcggcat ttttcagttt tggttgttca actggaccaa cgcgatgttg aagtacgaag
    10741 gactttcagc aaaggacttt gatatcgagt cagtacctaa ggattttgat cgttctctga
    10801 ttcttcccga aaactatacg gatgccgcgc acgaacaaat cccgcaagct aagatcaatg
    10861 cgcagcaaag aatcacgatg actcaacttc gtcgacttac agttccacaa taa
//
LOCUS       DASSFP010000194         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_347171, whole genome shotgun sequence.
ACCESSION   DASSFP010000194 DASSFP010000000
VERSION     DASSFP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347171"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFH91_04205"
     CDS             complement(<1..272)
                     /locus_tag="VFH91_04205"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6862219.1"
                     /translation="MIRKYLLGIVLMTLAGTLAAVPAFAQVGELRGHVYMQQADGTKA
                     PLADAQIDVFRTDVNAKYNTKTNKKGEFVFAGLPFVGTYTIAASHPT"
     gene            complement(387..1109)
                     /gene="queC"
                     /locus_tag="VFH91_04210"
     CDS             complement(387..1109)
                     /gene="queC"
                     /locus_tag="VFH91_04210"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HET6862220.1"
                     /translation="MAIPALTAEEASSLAVCLVSGGMDSCVTVALAANENDELAFLHA
                     SYGQRTADKERQSFEALADYFRVDQRLVISLEHLARIGGSSLTDSTIPIAEADLNSKM
                     IPSSYVPFRNAHLLAAATSWAEVIGASRIYIGAVAEDSSGYPDCRPEFYSAFQTVINV
                     GTKPETRIEIKTPVIQMKKSEIVRAGIDLGAPLNLTWSCYRESDKACGECDSCALRLR
                     AFREAQVVDPIPYLLDTKRLAY"
     gene            complement(1133..1684)
                     /locus_tag="VFH91_04215"
     CDS             complement(1133..1684)
                     /locus_tag="VFH91_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HET6862221.1"
                     /translation="MRCVWCDSEYTFTGGSQMSIEEVMDHIRRFDCKLVEVTGGEPLA
                     QKEAFSLITRLCDESFEVLVETGNYVSTEAVDQRAKIILDVKCPASGEEPRNDWSNLP
                     RLRKDRDEVKFVVANVDDWNYATKIIKDYDLENRALSILISPVWDQCNLQELANLVAS
                     SGLNVRMQLQLHKYIWGPEVKGV"
     gene            2071..3000
                     /locus_tag="VFH91_04220"
     CDS             2071..3000
                     /locus_tag="VFH91_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6862222.1"
                     /translation="MTDRAEDIQRLVKLGATRLLSRAEDNRSVKLDNLCLRITSAVEK
                     YLLRDDPSCSSHEIEQFIDALQADDLCLIIACEQSDEKAWTDLVERFTATVRSAARSA
                     SSNEDTAEDLVQSIWAELYGLRTKEDGKRASKLAYYSGRGSLAGWLRAVVAQLAVDSF
                     RKQSRLVQAEEDAELDRLANEVSFGEGQIALTTATQNPEETISNKLTNAQLDDALSRS
                     VKDLGPDDRLLVKLYYFDNLKLREAGAAMGVHEATASRRLTKIHNELRRRVSDILIQE
                     KGWTKAETERSFAEAAQHLDSDLGILLRNGSVS"
     gene            3082..>3401
                     /locus_tag="VFH91_04225"
     CDS             3082..>3401
                     /locus_tag="VFH91_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6862223.1"
                     /translation="MKEEINKEMDVLLRRLSRRDGTTGPNGENSSASNHLDVDELNAY
                     SENALPEKTRLRYTEHLAECAQCRRIVAQLTQSAAVDLPKGEKPKASWLASILGKFLS
                     PFTWR"
BASE COUNT          880 a          835 c          873 g          813 t
ORIGIN      
        1 gttggatggc tcgcagctat cgtgtaggta ccaacaaagg gaaggcccgc gaagacaaac
       61 tcgcccttct tatttgtctt cgtattgtat ttggcattga cgtcagttcg aaaaacatcg
      121 atctgagcgt cagcaagggg agcctttgta ccgtcggcct gctgcatata aacatgtcct
      181 cgcaactcgc cgacctgagc gaaggctgga acagctgcaa gagttccagc gagtgtcatg
      241 agaactattc caagaagata ttttcgaatc atttactgat tcctccaata atggtgccgg
      301 cgatcaaacg ccgagataga ggcttaggat gcgctgacat ccctttccgg ttgttaataa
      361 gtttgccact gagaagcgct ctaaacttaa taagcaaggc gcttagtgtc aagcagatag
      421 gggataggat cgacgacttg ggcttcgcga aacgccctta acctgagagc acaggaatca
      481 cactcgccac acgccttgtc tgattcgcga taacaagacc acgtcaggtt taaaggagca
      541 ccgagatcaa tgcctgcgcg cacgatctcc gatttcttca tttggatcac gggagttttg
      601 atttcgatcc gggtttcagg tttagtacct acgtttatta cagtttgaaa cgccgagtaa
      661 aattctgggc gacaatcagg atagccagac gagtcttcag ccaccgctcc gatataaatt
      721 cgactcgccc caattacttc tgcccaactc gtagcagcgg ccagcagatg agcgtttcga
      781 aaaggcacat aacttgaggg aatcattttt gagtttaaat ctgcctcggc aatggggatt
      841 gttgaatcag tgagagaaga gccgccgatt ctcgccagat gctcgagcga tatcacaaga
      901 cgctgatcga cccggaagta gtcagccaaa gcttcaaacg attgcctttc cttgtccgca
      961 gtgcgttgac cgtaagatgc atggagaaag gcaagttcat cgttttcgtt tgccgcgagc
     1021 gcaactgtga cgcacgaatc cattccgcca gacacgaggc agacggcgag actcgaagcc
     1081 tcttcagcag tcaatgctgg tattgccatt gttaattctt cagtgccacc catcaaacac
     1141 ccttaacctc aggtccccaa atgtatttat gaagctgcag ttgcatgcga acgttcaatc
     1201 cgcttgaggc tactaagttt gccaattctt gcaagttaca ttgatcccaa acgggggata
     1261 ttagaatcga taaggcgcga ttttccaggt catagtcctt aatgatcttt gttgcatagt
     1321 tccaatcgtc aacatttgca accacaaatt tcacttcgtc gcgatccttt cggagtcgtg
     1381 gcaaattgga ccagtcgttg cggggttcct cgcccgaggc cggacactta acatccagaa
     1441 taattttcgc gcgctggtcc accgcttccg tcgacacgta attacctgtt tcgaccagta
     1501 cttcaaagct ttcgtcgcac aatcgggtga tcagagagaa cgcctctttc tgggccagcg
     1561 gctcgccgcc ggtgacttca acgagcttgc aatcaaatct gcgaatgtgg tccattacct
     1621 cttcgatcga catctggctg ccgccggtaa acgtgtattc gctgtcgcac caaacgcaac
     1681 gcataggaca gccagtgaga cgcacgaaag agcatggccg tcccgcatgt gtcgactcgc
     1741 cctggatcga gcggaaaatt tcagtaacgc ggagcatagt cgttggaaaa aggtcgggtc
     1801 gaatctagct caggtggtgt aggctggtca aaagtaccta tccttgcagt ttcagcatca
     1861 cctaaacact catcgcggca tcaaaagtct gggctctggt aatgattcct cggacgcgtc
     1921 cattgtttgg acaactcttt cagtcgagat ttgagaattc ctactggaac ctgcggaatt
     1981 agatgtttat gagctcagcc agtgtccacg gcaaatggcc attagagccg aagatcgctc
     2041 gtgataggat tgaatgagtt ggtatcccct gtgacagaca gagctgaaga tattcagaga
     2101 ctggttaaac ttggcgcgac cagattgctt tcgcgcgccg aggacaaccg gtctgtaaaa
     2161 ctggacaatc tttgtctccg gataacctcg gccgttgaaa aatatcttct tcgcgacgat
     2221 ccgtcatgtt cctcgcacga aatcgaacag tttattgatg ctcttcaagc tgatgatttg
     2281 tgcctcatca ttgcttgtga gcaatccgac gaaaaagcat ggaccgatct ggttgagcgt
     2341 tttaccgcaa cggtgcggtc agccgcccgt tcagcaagtt caaacgaaga cacggccgaa
     2401 gaccttgttc aatcgatctg ggccgagctc tacggactgc gaacgaagga agatggcaaa
     2461 cgagctagta aattggcgta ctactcagga cggggctcgt tggctggttg gctgcgagcg
     2521 gtagttgccc aactcgccgt tgattccttc cgaaaacaat cgcggttggt ccaagccgaa
     2581 gaagatgcag aattggatcg tcttgcgaat gaagtgtcgt tcggagaagg gcagatcgca
     2641 cttacaacag caactcagaa ccccgaagaa acaataagta acaaactgac taacgctcag
     2701 cttgacgatg ccctgagcag atctgtaaag gaccttggcc cggacgatcg cttgctcgtc
     2761 aaactctatt attttgacaa tttgaagttg agagaagccg gggccgcaat gggagttcac
     2821 gaggctactg caagtagacg attaaccaag attcacaatg aactgcgccg gcgcgtgagt
     2881 gacattctga tccaggagaa aggctggacg aaagcggaaa cagagcgatc ttttgcagag
     2941 gcggcacagc atctcgatag tgatctgggc atacttttgc gcaatggaag tgtttcataa
     3001 cagcgccgca actgaagtag gacttttgag tgcaaggttt tactggtaag ttcgttcttc
     3061 agatggcgat ttactgtggt aatgaaagaa gaaattaaca aagagatgga cgtgctgctt
     3121 cgcaggttaa gccggcgaga cggtacaacc gggccgaacg gtgaaaacag ttcagcgtcg
     3181 aatcatttgg acgtcgacga gttaaatgcc tactcggaaa acgcactacc cgagaaaacc
     3241 cggctgcgtt atacagagca cctggccgaa tgcgcacagt gtcggcggat tgttgctcaa
     3301 ctaactcaat cggctgcggt tgacctgccg aaaggtgaga agcctaaggc atcatggctg
     3361 gcgagcattc ttggaaagtt tttgtcgcca tttacctggc g
//
LOCUS       DASSFP010000195         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1822933, whole genome shotgun sequence.
ACCESSION   DASSFP010000195 DASSFP010000000
VERSION     DASSFP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822933"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFH91_04230"
     CDS             complement(<1..245)
                     /locus_tag="VFH91_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862224.1"
                     /translation="MKTNTTKNLASAAMLVVLSPVLALALLVSVKTAGIQGNTVLLLL
                     LTLTGAVLSGINGFGRRTARAIPPSTVKVNPVDGPSE"
     gene            complement(605..2137)
                     /locus_tag="VFH91_04235"
     CDS             complement(605..2137)
                     /locus_tag="VFH91_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6862225.1"
                     /translation="MSDSSLLSAGAEIAEPGSQQLPISSIIRNVCEAANGGPDMLYEA
                     LIKMSLMVEATAPTYGLSIWAMSVDGKPSLRWAEGLQEEELAEGKKAVIDALASNGRP
                     IPANIGDVAICLLLALPTPLRDGAVIYGRCVRPLNETQAKELNALGDVAHMAHSHQLS
                     STPPLNSSKAQTERVAINALPGMVFSSRAMSEVARNVERIKDSDSTVLVTGESGTGKE
                     LIARAIHRISRRSENEFVPFNCSAVPAELIESMLFGHRKGTFTGASSDQMGLIRAAEN
                     GTLFLDEIGDLPLALQPKLLRFLQEGEIHILGDRAPRKVNVRVIAATHKDLEQLVKEK
                     LFREDLYYRIATLTVKVPPLRDRPEDISTLISHFITLYSRKNERNVSGITREAIQLLE
                     AYDWPGNVRELAAEMERLVLYADDDGYIRPENISSRIRPEDLKGDVQSGPESLDDLLE
                     SYERRVITETLKRHDCNVAKTSEALGLGSRQTLYKKLKRLAIDVGDFLQEEEEPGMQF
                     RA"
     gene            2603..>3645
                     /locus_tag="VFH91_04240"
     CDS             2603..>3645
                     /locus_tag="VFH91_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862226.1"
                     /translation="MTIHFGTSGWRAVIADEFTFAAVRRVTEAICLQFSSDQAAQPLI
                     VAHDTRFLGEDFAEQCAQIIEQYGFNALRCVGPTPTPAVSHAIRSNKAVCGINFTASH
                     NPPEYNGMKLSTADGAPALPAVTQKLESRLATVDQNTNQANKAGKTEDHDPRQAYLAD
                     LKDKIRFDVIAKANARYAYDPMWGTGRGYLDGVLREHGLTVTTIHDWRDVLFGGRAPE
                     PEESHLHELRQVVNQQKCVLGLATDGDSDRFGVIDSNGRFITPNQLIAILFDYLAETR
                     NWTGGVARSVATSHLVDRVAKLRGRPVYETPVGFKYIGELINEDKIILGGEESAGLSI
                     KGHYPEKDGVLAC"
BASE COUNT          852 a          943 c          921 g          929 t
ORIGIN      
        1 ctctcagacg gaccatcaac tgggttgact tttactgttg acggcggaat agccctggcc
       61 gtgcggcggc cgaagccgtt tattccgctt agcacagcac cagtaagagt cagcaacaac
      121 aacagcactg tgttgccctg aatccccgct gtctttacgc tgaccaacag ggccagtgcg
      181 agaaccggtg acaggactac gagcattgct gctgatgcca gattttttgt ggtgtttgtc
      241 ttcatagctt gtggtctgct cgaccaaatt tgaagcgatg gcttggtgcc tcgcgacgta
      301 catcccatta caagggacgt gccactgagt taatgccggg aaaatgaggt tttgcttgga
      361 tcttgctcga ggttttgtag cccgcggttg acaactctct gaccagagct gtggataacc
      421 gatggaccag gagtgcgaag tgggccagtt tgttaaggtt tgctggatgt cgcccacagt
      481 tgacaagttc aagtcacttc gcagcctccg ggttgacggc tggcgaacgt aatcgagaac
      541 caaaataaaa aagcatccga gcaaagcccg aatgccaggt aactcactta caggtggaag
      601 cgggtcaggc gcggaactgc atgcctggct cctcttcttc ctgaagaaaa tctcctacgt
      661 caatagccag ccgctttaac ttcttataga gcgtctgtcg cgaaccgagg cccagtgcct
      721 cactcgtctt cgctacatta cagtcatggc gcttgagcgt ctcggtaatt acccggcgct
      781 cataactctc aagtagatcg tccaggctct ctggtccaga ttgaacgtca ccttttagat
      841 cttcaggacg aatgcgtgaa ctgatgtttt cgggccgaat ataaccgtcg tcatcagcgt
      901 acaagactaa acgttccatc tcggccgcaa gctcgcgaac attcccgggc caatcatagg
      961 cttccagcag ctgtatcgct tcccgggtga ttccggagac attccgctcg ttctttctcg
     1021 aatacagcgt gatgaaatgt gatatcagcg tggaaatatc ttcgggccga tctcttaacg
     1081 gcggtacttt taccgtgagc gtcgcgattc tgtaatagag atcttcacga aagagctttt
     1141 cctttaccag ttgctcgaga tctttgtgag tggcggcaat cactcttaca ttgactttcc
     1201 tcggcgccct gtcgccaaga atgtgaatct cgccctcctg taaaaatctt aacagctttg
     1261 gttggagcgc taagggtagg tcgccgatct catcgagaaa cagcgttcca ttttctgccg
     1321 ctcgtatcag tcccatttga tctgatgaag ctccggtgaa agttcctttg cggtgaccaa
     1381 acaacatact ctcgatcaat tccgcgggaa cagccgagca attgaatggg acaaactcgt
     1441 tttccgatcg gcggctaatt ctatgaattg ccctggcgat caactccttg ccagtgcctg
     1501 actcgccggt tacgagcaca gtcgaatcag aatctttaat gcgctctaca tttctcgcga
     1561 cttcagacat agcccgactg ctgaagacca tgcccggcaa agcattgatg gcaacccttt
     1621 cggtttgtgc ttttgaactg ttgagaggtg gcgttgagct taactggtga gagtgggcca
     1681 tgtgcgccac atcacctaat gcgttcaact cttttgcttg agtttcatta agcggacgaa
     1741 cgcacctgcc gtaaataact gcgccatctc taagcggagt aggcaaggcg agtaacagac
     1801 agattgctac gtcaccaata tttgcgggaa ttggtctacc gttactcgca agcgcatcga
     1861 tgactgcttt tttcccttca gctaattctt cttcttgcaa tccttcggcc caccgcagac
     1921 tcggtttgcc gtcgacggac atcgcccaga tcgacaaacc ataagttggc gcagtggcct
     1981 caaccataag gctcattttg atgagagcct catagagcat gtccggcccg ccattggcgg
     2041 cctcgcatac attccggatg attgaactga taggtagttg ttgggaacca ggctcggcga
     2101 tttccgcgcc cgcactaagc agcgaagagt cagacatctt cacctatcct tcgggttctt
     2161 ttaaagcatt gatccaatga gtgagatgtt ggatcggatt gagtcgggac aagcagtacc
     2221 gatccgggtt ggcgactaat ctaactgcct ttcacccgca ccgccttgag tctaagggtc
     2281 gtccctttct actgtcaaca tgttttaacg cagtcgattt gagagcatca tttccgttcg
     2341 ccctgcgttc gccattttgc cgtcttaagc gctcttagtt caagtaaatg ttcaacttac
     2401 gtagttaaag cattgaggag aaatatcttt acacgcttaa gcacgggtgc tacgctcccg
     2461 ccacctcgca cctaagttat tgatttgaca ataggttggg aaacttggaa ccacattttc
     2521 ttggggctca gtttcgagga tatcgattat cttgtgggga aacccacgat agcaggttaa
     2581 gcagcctctt aattattaag gtatgactat tcactttgga acatctggct ggcgagcggt
     2641 aatcgcggac gaattcacat tcgccgccgt tcggcgtgtt accgaagcga tctgtctgca
     2701 atttagttct gaccaggctg cgcaaccgct aatcgtcgct catgacaccc gattcctggg
     2761 tgaagatttc gccgaacagt gcgcgcaaat tattgaacag tatgggttta atgcactccg
     2821 ctgcgttggg ccaaccccaa ctcctgcagt ttcccatgcc attagatcaa ataaggcagt
     2881 gtgcggtatc aacttcactg cgtcgcataa tcctccggaa tataacggga tgaagctctc
     2941 gaccgctgat ggagctccgg cgctacctgc cgtaacccag aaactggagt cgaggctggc
     3001 caccgtcgat cagaacacta atcaggcaaa taaggcagga aaaaccgaag atcacgatcc
     3061 gcggcaagct tatctcgctg atctaaagga caaaattcgt tttgacgtta tcgccaaggc
     3121 caacgccagg tacgcttacg accctatgtg gggcaccggt cgcggatatt tggatggagt
     3181 tctaagagaa cacggattaa ctgtcaccac aattcatgat tggcgggatg ttctcttcgg
     3241 tggcagagca cctgagccgg aagagagtca tctgcacgag ctccggcagg tcgtcaatca
     3301 gcaaaaatgt gttttgggac tggcaacgga tggcgattcg gaccgtttcg gcgtaatcga
     3361 ttcgaatggc cggttcatta cacccaatca gctaatcgcg attctcttcg attacctcgc
     3421 tgagactcga aactggactg gcggtgttgc tcgctctgtg gccacgtctc atctggttga
     3481 tcgagttgca aaattgcgag gccggccggt ttacgaaacg ccggtcggct ttaaatacat
     3541 tggagagctg atcaatgaag acaaaatcat tctcggtggc gaagagtcgg cagggttgtc
     3601 gatcaagggt cactacccgg agaaagatgg cgtccttgcg tgttt
//
LOCUS       DASSFP010000196         4767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1536653, whole genome shotgun sequence.
ACCESSION   DASSFP010000196 DASSFP010000000
VERSION     DASSFP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536653"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..1138
                     /locus_tag="VFH91_04245"
     CDS             362..1138
                     /locus_tag="VFH91_04245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6862227.1"
                     /translation="MIRNGLFGLIVGALLFIPLERFLPLRRNQRILRRGWTTDVAHFL
                     FTRSLADVATFIIVGSLIVLLHWLVSPTFQQVVASQSRVAQLTEAVMIANVGGYFGHR
                     LSHQVPFLWKFHKIHHSIDEMDWLAAARVHPLDQIVTKALTIVPLYVMGFSKATFGAY
                     LVLAVFWAVLIHANVKLNVGPLRWVITTPQFHHWHHSNEPAAYNKNFAGEIPLLDLLF
                     GTFYLPDTRMPASYGLSEVVPNGYLKQLLYPFRRQNLPTS"
     gene            complement(1139..1948)
                     /locus_tag="VFH91_04250"
     CDS             complement(1139..1948)
                     /locus_tag="VFH91_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET6862228.1"
                     /translation="MFLKNIFIYPIKSMKGASVSEATVEERGLRYDRRWMLIDSNNRF
                     ISQREQPRLALMSVEVTTNGLLVKSPADGQVAVPFHPVDPQSERVNIWDHISDSVTVS
                     HELDRWFSNVLGTSCRLVQMAPGDKRPVPAAYAVNDESVSFADEFPFMLLSEGSVEEL
                     NRRLDVPVQVNRFRPNFVVSGALPFAEDEWKRISIGSTSFHGVKSCARCVMTTVDQEQ
                     GIKAGPEPLKTLAGFREYDGRVLFGRNLIAAEVGGIVRVGDPIVATTDKGA"
     gene            2242..3285
                     /locus_tag="VFH91_04255"
     CDS             2242..3285
                     /locus_tag="VFH91_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HET6862229.1"
                     /translation="MKDTLEQIAAQLAAEVEHEQIVTWSNPQVNYRGIIAIHSTKLGP
                     AVGGTRFFNYPSDQLAVTDAIRLSRTMSYKSALAGLALGGGTAVIIGDAQNSDREEIF
                     REHGRFVNSLRGRFITAEDVGTRPSDMVIVRKETQFVGGLPDQSGDPSPVTARGVFRS
                     IQACAEHRWGTRELGNKTIAIQGCGSTGYHLGASLYEAGAKLVVSDLDSSKSQRFAEE
                     FHATVTTPTEIFDVRAEIFAPCALGGVINDETINRLKCEIVAGSANNPLLDDRHGDDL
                     HSRGIIYAPDCIANSGGIINGCRELLSWSREKASERVDGLYQVMLEILKASVKENAPP
                     FRIAYAKARQLLN"
     gene            complement(3286..3939)
                     /locus_tag="VFH91_04260"
     CDS             complement(3286..3939)
                     /locus_tag="VFH91_04260"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6862230.1"
                     /translation="MVAKIIGILSGFIIATISALGYWGIVLLMAIESACIPLPSEIIM
                     PFSGYLVSKGELNLWGVGIAGAVGCVVGSLVAYWVGMYGGRPFIEKYGRYILLSRHDL
                     DLADRWFAKYGEAIVFVSRLLPAIRTFIAFPAGVARMNLPRFIIYTFAGSLPWCLGLA
                     YVGQKLGEQWDKDETLKNWFHRFDFVIGIFGVIAVSWWIWRHIKHSRPTHVSKQEAD"
     gene            complement(3964..4584)
                     /locus_tag="VFH91_04265"
     CDS             complement(3964..4584)
                     /locus_tag="VFH91_04265"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET6862231.1"
                     /translation="MVARLREHYGIRDPRVLAAMRQIPRHVFVPEALQGRAYGDHALP
                     ISANQTISQPFIVARMTELLELGDDSKVLEIGAGSGYQTAVLSKVAAQVYAIERIGDL
                     AREAQSRIRQLGIYNATVKCFDGTLGWAAHAPYDGILVAAGGPAIPEPLVNQLKAGGR
                     LVVPVGNSRESQRLIRVIKTEKSFKQEDHGACAFVPLIGQHGWSDR"
BASE COUNT         1223 a         1252 c         1148 g         1144 t
ORIGIN      
        1 agtaccaatc ccgagatacc gaagctcgcc agccccatag cgctttgtct taagaaggtg
       61 cgtcgttttg agttaatcat tctggagact tccctttcgc cagtctatct gaggtcctga
      121 ttgtggtcgg cgagaaaaaa agtagtttca tcgcccgtca caatgaacaa cactatgaca
      181 atggctgttg tgacaaagat gtgacgaagc ggcccaaatg aatgatgaag tgaaaccgcc
      241 aacttcccga tctaaagttg tcgaattcgg gaaatcggtg aaaaaccccg aatagtctga
      301 gggacgagcc tgcaatgctg gatgaaactt tttgcagcta cgtgtcgtct taagaaacac
      361 gatgattaga aacggcttat ttggactgat tgtcggcgcg cttttattta ttccgctgga
      421 acgatttctc cccttacgac gcaatcaaag aatactacgt agaggatgga ccactgacgt
      481 ggcccacttc cttttcacgc gcagcctcgc agacgtcgct acgttcataa tcgtgggttc
      541 cttaattgtt ttgctgcact ggctggtgag cccgaccttc cagcaagtcg tagcctcaca
      601 atcaagagtt gcccaactta ctgaagccgt catgatcgct aacgtaggtg gatattttgg
      661 ccaccgcctt tcgcaccagg ttccatttct ctggaagttc cacaaaattc accacagcat
      721 tgacgaaatg gactggctag cagcagctcg agttcatccg ctcgatcaaa ttgtaactaa
      781 agctctcact attgtgccgc tttacgtgat gggattcagt aaagccacct ttggggccta
      841 cttagtgctg gcagtgttct gggccgtgct gattcacgcg aatgtcaaac tgaatgttgg
      901 cccacttcgc tgggtcataa ccactccaca atttcaccat tggcaccaca gcaacgaacc
      961 ggccgcttac aacaagaact tcgctggcga gatcccccta ctcgatctgt tgttcggcac
     1021 tttctatctg ccagatacga gaatgcctgc gtcgtacgga ctttcagaag tggtgcctaa
     1081 tggctatttg aagcaactcc tttacccgtt tcgcagacag aatttgccaa cgtcttgatc
     1141 aggctccctt gtctgtggtg gcgacaatcg ggtcaccgac acgcactatt ccgccaacct
     1201 cagccgcaat caaatttcgt ccgaaaagaa ccctcccgtc atactcccta aatccggcga
     1261 gagtcttcaa cggttcagga ccagccttga ttccctgctc ctggtccacg gtggtcatca
     1321 cgcatcgagc gcaggatttc acaccatgaa atgaagttga tccaatagaa attctcttcc
     1381 actcgtcttc tgcgaaaggt agcgcgccgc taacaacgaa gttaggtctg aaacgattaa
     1441 cttgtaccgg aacgtccagt cgacggttca gctcttcgac tgatccttcg ctgagcagca
     1501 tgaaaggaaa ctcatcggca aaactaaccg actcatcgtt gacagcataa gctgcgggta
     1561 cgggccgctt atcgccaggc gccatctgaa ccaggcggca tgacgttccg aggacgttgc
     1621 taaaccaacg gtccaactca tggctcaccg ttaccgaatc gctaatgtga tcccaaatgt
     1681 tgacccgttc cgactgaggg tctacagggt gaaaggggac agctacttgt ccatctgccg
     1741 gcgactttac cagcaatcca tttgttgtca cctcaacgga catgagggca agccgcggtt
     1801 gctcacgttg cgagatgaaa cggttgttcg aatcaataag catccaccgg cgatcatatc
     1861 gaagccctct ctcttcaact gttgcttccg atacggacgc acctttcatg gatttgattg
     1921 ggtagatgaa tatgtttttg aggaacacaa cgggattcct ttgcagtgcg ccgactcggc
     1981 gccggtttag tattgattgt aatctgggag taacaaagac ggcgccggac gccgcactcc
     2041 cccaaaatgg cttcgtatct gcatcgatag tataaaccaa tcgattagtt catcggcgat
     2101 ccatttaagc attcatcaga tccatgaaac cgtacgccgg tcttcgtcct tggatttctt
     2161 acctttattg gaacctaaac caacatcaac gacgtttcac aacttgagga ttttcatatg
     2221 caacacaggg atgaaaaccg aatgaaagac acactcgaac agatagcggc acaactggca
     2281 gcggaggttg agcacgagca aatagtaacc tggtccaatc cgcaagtgaa ttatcgtggc
     2341 atcatcgcta tccacagtac caaactcgga ccagcagtag gcggtacccg gttcttcaac
     2401 tatccgtctg atcagcttgc cgtaacggat gccattcgcc tatcgcgcac gatgtcttac
     2461 aaaagcgcgc tggccggtct agcccttggt ggcggcacgg cggtcattat tggtgacgca
     2521 caaaattcag atcgtgagga gatttttcga gagcacggac gctttgtaaa ttccttgcgc
     2581 ggacgattca tcaccgccga agacgtggga acacgtccat ccgacatggt tatcgtccga
     2641 aaggagactc aatttgtcgg tggattgccc gaccaatcgg gtgatccatc accagtcacc
     2701 gcccgtggag tattccgatc tattcaagcg tgcgccgaac atcgctgggg aacccgagag
     2761 ctgggtaata aaacgatcgc gatccaggga tgcggttcga cgggttatca tctcggcgca
     2821 tctctgtacg aagccggcgc caaactagtc gtcagcgatc tggactcaag caagtcgcaa
     2881 cgatttgccg aagagtttca tgcaacagtg acgactccga ccgagatatt cgacgtacga
     2941 gcagagattt tcgctccctg tgcactcgga ggggtaataa acgatgaaac aatcaacagg
     3001 ctcaagtgtg aaatcgttgc gggctcggct aataacccgt tgcttgacga tcgccacggc
     3061 gacgatctgc attcaagagg aattatttat gcacctgatt gcatcgcaaa ctccggtgga
     3121 atcataaatg gttgtagaga gttactgagt tggagtcggg aaaaagccag tgagagagtg
     3181 gatggcctct accaggtgat gttggagatt ctaaaagcgt ccgtcaagga aaacgcaccg
     3241 ccttttcgaa tcgcttatgc gaaagccaga cagttattaa attaactagt ccgcttcctg
     3301 tttgctgacg tgagtaggtc gcgaatgttt gatgtgtcgc caaatccacc aggaaacagc
     3361 gataactccg aatattccga tcacaaagtc gaatcgatga aaccagtttt tcagcgtttc
     3421 gtccttgtcc cactgttcac ctaatttctg tcccacatac gcgaggccaa gacaccatgg
     3481 taaagaaccc gcgaacgtat agatgatgaa tcggggtagg ttcatgcgag caacgccggc
     3541 aggaaatgcg ataaacgttc taattgccgg taacagtcga ctgacaaaaa cgattgcctc
     3601 accatatttc gcaaaccatc gatcggccaa atcaaggtca tgccgtgaca gcaggatgta
     3661 acggccgtac ttctcaataa agggccgtcc accatacatt cccacccagt aagcgacaag
     3721 agaacccacg acgcatccga cagctccggc gatccctacg ccccaaagat tgagttcacc
     3781 tttggacacc agatacccgg aaaacggcat tatgatttct gagggtagcg ggatacacgc
     3841 ggattcaatt gccatcagca ggacgatgcc ccagtatccc agagctgaga ttgtggctat
     3901 gatgaaaccg gatagtattc caattatctt tgcgaccatg ttttagagaa ccaagtattg
     3961 aagttatcga tcggaccagc catgttggcc gatcagtgga acgaaggcac aagcgccatg
     4021 gtcttcctgc ttgaaagatt tttcagtctt gatgactcgt attagcctct gagactctcg
     4081 ggaatttcct accgggacca cgagtcgtcc acctgcctta agttggttta ccagaggttc
     4141 aggaattgcg ggaccaccag cagcaaccaa aattccatca taaggagcat gcgcggccca
     4201 ccccagcgtt ccatcaaagc acttaacggt ggcattatag attcctaatt gacgaattct
     4261 gctctgagcc tctcgcgcca gatcaccaat tctttctatc gcataaacct gggcggcgac
     4321 ttttgaaagt acagccgttt gataacctga acctgctcca atctccagca ccttactgtc
     4381 gtcacccaat tcaagcaact cggtcatcct ggctacaata aaaggttgag agattgtttg
     4441 attggcgctg atgggcaatg catggtcgcc ataggctctg ccctgtagag cctccggcac
     4501 aaagacgtgg cgcggaatct gccgcatcgc cgccagaact cgcggatcgc gtatcccata
     4561 gtgctcacgt aaccgcgcaa ccatgcggtc tctcgggatt gaatattcct gaaacgatga
     4621 agacggttgt cggtttaagg gctgcgatgt gtgcaattca gtgattaact cttttgcaat
     4681 agttctgaat cttttagata gttccaggtc tcaagggctg taagcgcctg gtgatcggtc
     4741 atgtcactgc gcaatggtgt aacagag
//
LOCUS       DASSFP010000197         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_200532, whole genome shotgun sequence.
ACCESSION   DASSFP010000197 DASSFP010000000
VERSION     DASSFP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200532"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..515)
                     /locus_tag="VFH91_04270"
     CDS             complement(126..515)
                     /locus_tag="VFH91_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6862232.1"
                     /translation="MSKPKCRILCVDDHRDTSEMLQLLLAEENYEVHTAATMKEASAL
                     SLEIDFDLFVLDKRLPDGTGIELCEILHELKPDVPCIFYTGDAYEIHRHEAMEAGAAA
                     YVAKPDIEELINSVHKLLSQSECSAAA"
     gene            complement(972..1793)
                     /locus_tag="VFH91_04275"
     CDS             complement(972..1793)
                     /locus_tag="VFH91_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862233.1"
                     /translation="MKEKNIKVISFPSPVPFKADYQRVKSSYSIKEIKQLFGLSEKTI
                     KRWTEQGIIQAAATSTDAELTYDFQALTQFRRVRELRAQGHSIRQIEAELQGQLSLFR
                     TEPGRLTRLLTPFEEALLLHERGDVSATEHYLEAISEGDNIAEAYCNLGIINLEQGQL
                     TKALDNFTLSLKNDPRHVEAHYNLANLYYDAGNLNLAKLHYEAAAQIEPNFSLVYFNL
                     SLVYHKLGDLAAATATLEKYSALEPDDEDIEVLTQILKAMQKSLSLQQKQESSKK"
     gene            complement(1824..>2763)
                     /locus_tag="VFH91_04280"
     CDS             complement(1824..>2763)
                     /locus_tag="VFH91_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET6862234.1"
                     /translation="QARHSAGESPRFALRRRPFMRSIWKGHIRFLLVAIPVRVYNAIE
                     TGEKIQFNQLHREDFGPIGYDKRCKKCGEVVTNDQITKGYQYEPDRYAIVEPDDVAKI
                     KIKTTKAVDIVGFVDRSEIPTTFYDAPYFAGPDGAVSEKPYALLREVMKQTGKVAIGK
                     VVLRDREDLVAVFPHEDGLVLQKLHYPHELRKMEDVPDLEGAKNLTAQKLNKNELKLA
                     ATLVQEMVTTLDEIDTEDNYHTALRKLIDSKIKGNEVVEFEVEELPRLDIMSALKKSL
                     QQSNRKPMVKAPSVAAKKKPEITLVKPKVAKKRKAS"
BASE COUNT          613 a          676 c          674 g          800 t
ORIGIN      
        1 ctttggcctt cggcctttga tcttcgtgct ccccaactgt ggattgactt attgctagtg
       61 attgggaacc agcgtcaaag accaaaggcc gaaggccaaa gatcaaagat caaagctcaa
      121 acccgttatg ctgctgccga acactcgctc tggctcagca gcttatggac cgagttgatt
      181 agttcctcta tgtcgggttt agcaacgtat gctgcagctc cggcttccat cgcctcgtga
      241 cggtgaattt catacgcatc ccctgtgtaa aagatgcaag gaacgtcagg ctttagttca
      301 tgtagtatct cgcacaactc aatgccggtc ccatcaggca accgcttatc aagtacaaat
      361 agatcgaagt caatttccag ggagagagca gatgcttcct tcatggtcgc tgcggtgtga
      421 acttcgtaat tctcctcggc cagcaacaat tgcagcattt cagacgtatc gcggtgatcg
      481 tccacacaaa ggatcctgca ctttggttta gacattcagg ggcacctcct tgttcgggtt
      541 aaaattattc agcaatcaag ggtgattgaa gcattgcggt tgacttggat tataaacgtg
      601 gccggacttc tgcgctatct cggagactga tctccccaag gcaaaagtca tctcgccacg
      661 gcaagaatct attgatcaat tgtcatttga tatttatcaa ttattagttg gtgcaacgcg
      721 atcccgttgc tctggggctt tgcgcatggc ctacgtgaaa attggaatcc gcacaccaaa
      781 ggatgaaggc ccaggtccaa accaattaat aactgataat tatcaattta caattgatca
      841 atagtgggtt tctggaacag tggctcgatt ggcgatttgc ttaagaagta cgaggtattc
      901 aggatcgttc acctttcttg cgatccccaa tacttgcata agtggtgggc agccctcctg
      961 cccaccgctg tttacttttt ggagctctct tgtttctgct gaagacttaa agacttctgc
     1021 atcgccttta gaatctgcgt taacacttcg atgtcttcgt catccggctc gagtgcgctg
     1081 tacttctcga gcgttgccgt tgccgccgcc agatccccga gcttgtggta gaccagcgac
     1141 aagttgaagt acacaagaga gaagtttggt tcaatctgcg ctgctgcttc gtaatgtagc
     1201 tttgccagat ttagattgcc cgcatcgtag tacagattgg ccaggttgta gtgagcctca
     1261 acgtgtcgag gatcattctt caaggagaga gtaaagttgt cgagcgcttt tgtgagttgt
     1321 ccctgttcga gattgataat gcccagattg caataagcct ctgcgatgtt gtcaccttca
     1381 cttattgctt ccaaataatg ttcggtagct gaaacatcac cacgctcatg caaaagaagc
     1441 gcttcttcga aaggcgtaag taggcgagta agtctgccgg gctcggtccg aaacaggctc
     1501 aattgcccct gtagttccgc ttcaatttgc cggatgctgt gaccttgcgc tcgcaactcg
     1561 cgaacgcggc gaaactgagt cagcgcctga aagtcgtaag tgagttccgc gtcggttgat
     1621 gttgctgccg cttgaatgat gccctgttca gtccaacgct ttatagtctt ctcactgaga
     1681 ccaaacagct gtttgatctc cttgattgag taagaagatt tgacgcgttg atagtccgcc
     1741 ttgaacggga caggtgaggg aaacgaaatt accttgatgt tcttttcttt catctaactc
     1801 gcgaaatcgc cagttacctc ttattacgag gcttttcgct tcttggccac cttgggctta
     1861 accagagtga tctctggctt ctttttggca gcaaccgatg gtgctttgac cattggttta
     1921 cggttcgatt gctgcaaact ctttttaagc gcgctcataa tatcaagtct aggcagttct
     1981 tcaacttcaa attcaacgac ttcattgcct ttgatcttcg agtcgatcag ttttcgcagc
     2041 gccgtatgat agttgtcttc agtgtcgatc tcgtcaagag tcgtcaccat ctcctgtacc
     2101 aacgtggcgg cgagtttcag ttcgttcttg tttagctttt gtgcggtcag gttctttgcg
     2161 ccttcgagat cgggcacgtc ttccatcttg cgcagttcgt gggggtaatg aagtttctgc
     2221 agtacgagtc catcttcgtg tggaaaaacg gctaccaggt cctctcgatc tctcagcacg
     2281 actttgccga tagcgacttt tccggtctgt ttcattactt cgcgcagcag ggcgtacggt
     2341 ttttccgaga cggcgccatc cggtcccgcg aagtaaggtg cgtcataaaa tgttgtaggg
     2401 atctctgagc gatcgacaaa tcctacgata tctacagctt tggtggtttt gattttgatt
     2461 tttgcgacgt cgtctggttc aacgatggca taacggtccg gctcatactg ataacccttc
     2521 gtaatctggt cgttggtcac gacctcgccg cattttttgc agcgtttgtc atatccgatt
     2581 gggccaaagt cttcgcggtg aagctggttg aactgaattt tttcgccggt ttctattgcg
     2641 ttatagacgc gaacggggat agcaacgagt agaaaacgga tgtgaccttt ccagatggac
     2701 ctcataaacg ggcgtctcct caatgcgaaa cgcgggctct cacccgcgct gtgtctcgcc
     2761 tgt
//
LOCUS       DASSFP010000198         4195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1687314, whole genome shotgun sequence.
ACCESSION   DASSFP010000198 DASSFP010000000
VERSION     DASSFP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687314"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..977)
                     /locus_tag="VFH91_04285"
     CDS             complement(129..977)
                     /locus_tag="VFH91_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6862235.1"
                     /translation="MTGPQTPSNRFLINPEYSVLYRSAAQLRWETNASSAYSFLAVID
                     GLLELSLDGVSLKLIKGQSRVLEPNISVTVTGKQVELLQLTISPALALQHAVTMKLIS
                     AKSMIAFSENVIEGDTRLNRILGDLTSELEAESPGKEIIVRALVEQLVVQLLRNYSIA
                     RLSDELELSRVGLVDRRIRRSVELMYTQLDQDLSLKDVAAASYLSPFHFARLFKKITG
                     STPHNYLAGIRSARAQQLLAESDLSITEIAARVGYLSASHFTKAFRQATGTTPRAFRK
                     ALINQN"
     gene            complement(1011..1805)
                     /locus_tag="VFH91_04290"
     CDS             complement(1011..1805)
                     /locus_tag="VFH91_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET6862236.1"
                     /translation="MPDLSANNSPLIIGHRGASAFAPENTMAAFNLAIESGADGIEFD
                     VRLTRDEIPVVIHDETLLRTGGVSQRVSDLSLEDLKQIDVGGWFGAKNRNCNFTGERI
                     PTLENLLRWAESNNARLYLEMKSDASRRQNLAGAVTELLKHFDSERVVIESFDLEALK
                     IIKVMAPHQKTAALFEPSLATPPLIRARRLIELAREVEADEIALHYRLVNKRIVKLAL
                     ENGLAVVVWTVDEPKWLDTARSLKVSAVITNNPKLLVVQRNSSVVG"
     gene            complement(1824..2681)
                     /locus_tag="VFH91_04295"
     CDS             complement(1824..2681)
                     /locus_tag="VFH91_04295"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET6862237.1"
                     /translation="MSFEFNKRLVSSVMAIALCVAPAMGQKAIAGRSSMPFEPAEELF
                     YDAEFSKSVLRNLDVAEFKFRTTRTPINSATSESNNLYSLTFSAEVSSKGFFTKLFNL
                     RFRERVESVVEPISFTARKTTILDEQGKRVRQSETIFDPAKGQLFWTQKDPTDPSREP
                     RNVTTQFSGQLQDVLSAIYFIRTQPLSVGKSFEMFIGDSGHVYRVPVQVVERKRMKTI
                     FGKVHVLRVDPDIFGPDSLIEQEKGQLSLWITDDNRRIPVGVKIKSDYGTFDIKLKRH
                     VNNLEAARK"
     gene            complement(2678..3232)
                     /locus_tag="VFH91_04300"
     CDS             complement(2678..3232)
                     /locus_tag="VFH91_04300"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET6862238.1"
                     /translation="MRRGGTFIQRWRVPLGFVVAIVFLFFARPTPTALLIGACVSLPG
                     LAIRAWASGHIRKNTRLAVSGPYAFTRNPLYFGSFLVGLGFTIASGQWVLALMFAALF
                     LGIYLPVMRVESSTMAELFGQEYENYREAVPLFFPRLTPFRQGDAGKVKFDGSLYLRY
                     REYRAALGLIAAWGLLILKTYLIR"
     gene            complement(3253..>4195)
                     /locus_tag="VFH91_04305"
     CDS             complement(3253..>4195)
                     /locus_tag="VFH91_04305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6862239.1"
                     /translation="EILRDNPLLDRLIEVDTKALRRGLMSGETLRAPRQQLRRLRASA
                     FDVALDFQGLLKSASIARLSGARRVFGYTREGLREPASALLLSKKVHVPKQTHVIYKA
                     LALVESALNLQVPKSPDKLSFPIQINKEHQIEAQQVIGSSANYALLNPGGGWPTKLWA
                     PEKFGALADALWKTLGLQSVVTYGPGDLELARAVQHASSSPAVVLANVSLKGFYELAT
                     KAKVYVGGDTGPTHIAVAAGTPIVGLFGPTEWWRNGSPRPQDICVERNDIDCRVDCHR
                     RSCSNWICMDISVERVLDAVAQRINAAEAPQALTLAG"
BASE COUNT         1051 a         1088 c         1048 g         1008 t
ORIGIN      
        1 gggtgaggtg gggaagcgac ggatcttgct cattattggc gagatattgc tacgacacga
       61 tcgggtccgc ccgctgttcg ataccaccga cttgaaggcg gcggtgtctg gattgcggag
      121 cctcaaaact agttctgatt gattagagcc tttctaaacg cgcgaggggt agttccggtg
      181 gcttgacgga aggccttggt aaagtgactt gcacttaaat aaccaactcg tgctgcaatc
      241 tcagttatcg aaagatcact ttcagccagc agctgttgag cccgcgccga tcggatgccc
      301 gccagatagt tgtgaggcgt gctgccagtt atcttcttga acaatcgcgc aaaatgaaaa
      361 ggcgagaggt acgaggctgc cgcaacgtcc ttcaatgata gatcctgatc taattgcgta
      421 tacatcaact caactgaccg gcgtattcgt cgatcaacca gcccaacccg cgacagttcg
      481 agctcatctg atagccgcgc gattgaataa ttccgaagca gctgaaccac cagctgctcc
      541 accaaagcgc ggacaatgat ttctttaccc ggactctctg cctccaactc ggaagttaga
      601 tcgccgagaa tccggttcag tctcgtatcg ccctcaatga cgttctccga gaaggcgatc
      661 atcgatttag ctgaaatcaa cttcatcgtg accgcatgct gcagcgcgag tgcaggcgag
      721 atcgttaact gcagaagttc aacctgcttt ccggtcacgg taacacttat gttcggctca
      781 agaactcttg actgaccctt tatgagttta agagaaaccc catcaagcga aagctccagt
      841 aggccgtcaa tgacagccaa aaaagaatag gcagagcttg catttgtttc ccacctcagc
      901 tgcgctgctg atcgatagag gaccgagtat tccggattga tcaggaatcg atttgaagga
      961 gtctgcggac cagtcatcga acccactgta gtcgaatcgg aatgttagct tcaaccaacg
     1021 actgagctgt tccgctggac caccagcagt tttggattgt tagttattac tgctgacact
     1081 ttgagcgagc gagcagtgtc tagccatttc ggttcatcga ctgtccagac gacaactgca
     1141 agtccattct caagtgcaag tttaacgatg cgtttattga ccagtcggta atgaagagct
     1201 atttcgtcag cttctacttc gcgtgccaac tcaatcaatc gtctcgctct aattaacggt
     1261 ggtgtcgcca aagatggctc aaataaagca gctgttttct ggtggggagc cataaccttg
     1321 atgatcttaa gtgcctcaag atcaaacgac tcaattacga ccctttccga atcgaagtgc
     1381 tttagcaact cggtaacagc ccccgccagg ttctgtctgc gactcgcatc cgatttcatc
     1441 tcgagatata gacgagcgtt gttcgattca gcccagcgaa gaagattttc cagggtcgga
     1501 attctttcac ccgtgaaatt acagtttctg tttttggctc cgaaccaacc tccgacatcg
     1561 atctgtttaa ggtcctcgag cgacaaatca gaaacgcgtt gagaaacgcc gccagttcta
     1621 agaagagtct cgtcatggat aacaacggga atttcatcgc gagtcaggcg gacgtcgaac
     1681 tcaataccgt cggctccgga ttcaattgcc agattgaacg ccgccatggt gttctcaggc
     1741 gcgaacgctg aagcaccacg atggccaata atcaggggtg aattatttgc ggatagatca
     1801 ggcacgctgc atcaacgggc gggttattta cgtgcagctt caagattgtt tacgtgtcgc
     1861 ttcaacttga tgtcgaaggt tccgtaatca cttttaattt tgacgcccac aggtatgcga
     1921 cgattgtcgt cagttatcca gagtgataac tgaccttttt cctgctcaat caaactgtca
     1981 ggtccgaaaa tatccgggtc cactctcaaa acgtggactt tgccgaatat tgtcttcatc
     2041 cgtttgcgtt cgacaacctg gaccggtaca cgataaacgt gaccgctgtc gcctatgaac
     2101 atttcaaaag actttccgac cgaaagaggt tgtgtccgaa taaagtagat agccgagaga
     2161 acatcctgca gttgtcctga aaactgcgtc gtaacatttc gtggttctcg tgaaggatcg
     2221 gttgggtcct tttgcgtcca gaagagttgg cccttcgccg gatcgaaaat cgtctcgctc
     2281 tgacgcacgc gtttgccctg ctcgtcgaga atggtggttt ttcttgccgt aaaacttatc
     2341 ggttcgacaa ccgactcgac tcgttcacgg aaccttaagt taaatagctt tgtaaagaat
     2401 cccttgctgg aaacctcagc gctgaatgtg agactgtaaa ggttgtttga ttctgacgta
     2461 gcgctattaa ttggtgttcg agttgttcgg aacttgaact cggccacatc aagattccgt
     2521 aaaacggact tcgagaactc agcgtcgtag aaaagctcct cagctggttc aaacggcatc
     2581 gagcttcgac ccgcaatagc tttctggccc atcgctggcg cgacgcagag ggcgattgcc
     2641 attaccgatg acactaaccg tttgttaaat tcaaaactca tcgaatcaaa tacgtcttta
     2701 aaattaagag gccccaggct gcaatcaggc ccagagcggc gcgatattcc cgatagcgaa
     2761 gatacaggct cccgtcgaac ttcacttttc cggcgtcccc ttgtcggaat ggtgtgagac
     2821 gtggaaagaa caacggtacg gcttctctat aattctcgta ctcctgaccg aaaagctcgg
     2881 ccatagtgga ggattcgacg cgcatcacgg gcaaatagat gccaagaaaa agcgccgcga
     2941 acattaacgc caggacccat tgacctgacg caatagtgaa tcccagacct acgagaaagc
     3001 tgccgaaata gaggggattt ctggtaaagg cgtaaggtcc ggagacggcc aatcgcgtat
     3061 tttttctgat gtgaccagat gcccatgctc ttattgccag acccggcaag gacacacaag
     3121 caccaatcaa aagggccgtt ggcgtaggtc tcgcaaaaaa cagaaacacg atggcaacta
     3181 cgaagcctaa cggaactcgc caacgttgaa tgaacgtacc tccgcgtcgc atgcccaaat
     3241 aagtccttcc gcttaacctg cgagtgttag tgcttgagga gcttcagcag cgttgatcct
     3301 ttgggcgacc gcgtccaata ctcgctcaac acttatgtcc atacagatcc agttcgaaca
     3361 cgaacggcga tggcagtcga ctcgacaatc gatgtcattc cgctcaacgc agatatcttg
     3421 cggcctggga cttccattcc tccaccattc cgtcggacca aagagtccaa caattggggt
     3481 gccggctgca accgcgatat gagtcggccc ggtatcacct ccaacataga cctttgcctt
     3541 agtggccagc tcgtagaagc ctttcaaaga aacattggcc aggacgactg cgggtgaact
     3601 cgaggcgtgc tgcactgctc gggctaactc taaatctcct ggtccgtaag tcacgactga
     3661 ctgaaggccg agagttttcc acagggcatc agcgagggct ccgaattttt cgggcgccca
     3721 aagtttcgtc ggccatcctc ctccggggtt aagaagtgca tagttcgccg agcttccgat
     3781 gacttgctgt gcttcgatct ggtgttcctt attgatctgg atagggaagg aaagcttgtc
     3841 cggagacttt ggaacctgga ggttcagagc gctctcaacc agggcaagag ccttataaat
     3901 cacgtgggtt tgtttaggga catggacttt tttcgaaagg agtaaagcgc tggcaggttc
     3961 gcgcaggcct tcgcgggtat atccaaaaac acgacgcgcg ccagaaaggc gcgcgattga
     4021 agctgatttt aataatccct ggaaatctaa tgcgacatcg aaggctgagg cacgtaaccg
     4081 acgtaactgc tggcgcggtg cccgcagcgt ctccccagac atcagcccgc gcctcaacgc
     4141 cttcgtgtcg acttcgatga ggcgatccag aagcgggttg tctctcaaaa tttca
//
LOCUS       DASSFP010000199         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2131876, whole genome shotgun sequence.
ACCESSION   DASSFP010000199 DASSFP010000000
VERSION     DASSFP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131876"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..331
                     /locus_tag="VFH91_04310"
     CDS             29..331
                     /locus_tag="VFH91_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET6862240.1"
                     /translation="MVKVALWVQLDAKPGKEKDVEDFLMSGLALAENEPDTTAWFALR
                     LGPSTFGIFDAFPSDAGRSAHLSGPIAAALMSKASELLAKPPTIVNVDVLAAKLPG"
     gene            812..1393
                     /locus_tag="VFH91_04315"
     CDS             812..1393
                     /locus_tag="VFH91_04315"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HET6862241.1"
                     /translation="MNAITQMVMQQIAGGASQQIAQRFGISESTANTAIQLAVPLILS
                     AMARNAAQPQGAENLHQALNSDHDGSIFNDLMGHLSNPQTANGAGILGHVFAGQQANV
                     ESNLAQAAGIDQSSAGGLMEMVAPLVMGAVGQAQQQNGLDASQLSNMLTVQQQQAEAT
                     APDVMGMLGSMLDQNKDGSAMDDLWRVAGNFFK"
     gene            1497..2003
                     /locus_tag="VFH91_04320"
     CDS             1497..2003
                     /locus_tag="VFH91_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6862242.1"
                     /translation="MKTKAVGIAVMLTLIAALMGCNRGPNDETLTQQVKAKISADPAL
                     ATQPVNVSVKDGVVTLAGNVNTATNKAKAEDLAKGVDGVKSVSNSLSVRPPIVNATPP
                     PVSPDTQLKTSVMAGLTKYGITTVSAEVVNGEVTLSGNIPRAKLQDAMKAANESHPKK
                     VVNKMTIK"
     gene            2017..2385
                     /locus_tag="VFH91_04325"
     CDS             2017..2385
                     /locus_tag="VFH91_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET6862243.1"
                     /translation="MELTEKYKPVIDIASQNGVNPQVAEQDGVLYITATTNNGSVKQQ
                     MWDKYGEIDPDYRSGDLVLNVEVEGGGYEEYTVQSGDSLSKIGSHWGKNWKEIWDLNR
                     DVIGANYNLIHPGQKLRIPK"
     gene            complement(2428..>3463)
                     /locus_tag="VFH91_04330"
     CDS             complement(2428..>3463)
                     /locus_tag="VFH91_04330"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862244.1"
                     /translation="AGADILTRVIDSIANNKPADAIAVVDDIVMRGHDLRSFCRDLLA
                     HFRDLLVTKVSGNEELLEAAVCEPVELKRQADLFSESDLVRFFHSLAETETKLRTATQ
                     PRYQVEIGIVKLMELRGVESISEILQRLATLEGSSSGKPGGGSSSSGNGSPAPRKGGA
                     GQINSGSRAASSSFSAAAVAPAFEMKTEVTVQSTAAIDEPATTDDGITTGSEMTVMDR
                     IKAALENQRKMLLVTVLEAAHAASIEDGELFVEFTPESRHFRDTLTKAENIKILREVC
                     REVTGQEAGVRIAVRDLQEQSSGPATREDDERIEKKKLRESAESNPIVQEMMRTFRGE
                     IVDVRRIDGK"
BASE COUNT          892 a          866 c          886 g          819 t
ORIGIN      
        1 actgccattc taatatttgg aggaactcat ggttaaagtt gctctctggg tgcagctcga
       61 tgcgaagcct ggaaaagaaa aggatgtgga agattttttg atgtcaggac tagcgttggc
      121 cgagaacgag cccgacacga cagcctggtt tgcattgcgg cttggtccat ccacttttgg
      181 gattttcgat gcttttccca gtgacgcggg acgtagcgca cacctttcgg gaccaatagc
      241 tgctgcccta atgtccaagg cgtctgagct gctcgcaaaa ccgccaacca ttgtcaatgt
      301 tgacgtactt gctgcaaaac ttcctggtta atgtttgacg cctgagatag tcgtatcggc
      361 gtaacaataa ttccacatcc ttacaaccgg ttgtaactcg ccggatgcct gtcgttggtg
      421 tagtcttttg ctcacgcaac caagatgtaa acaagaaatg cagcggcttt gatctgctgg
      481 caattctatt cttcgcactt cccccaaaga tgttttcatg cctgaagatc gagtaagcgt
      541 gaaccgagtt tgggatttga agtaaaaatc gatcccgaac ctactcgaaa agcaatccaa
      601 gagcctcgcg cgaactgagc ctcaagtcgt gcgcccaccg acttgtaact tgtttaagac
      661 cacgcgggtt ggcaggagcg ctcacaacat cgcatctatc ctgaaagacg tgtcgccatt
      721 gtccgaaggg gcaactaccg tgcgactgac gatgaagagt tccgcgaaat gttcttaacg
      781 tcgctaccct aattctagaa ggagtttcat catgaatgcc ataacccaga tggtgatgca
      841 acagatcgca ggcggtgcat cccaacaaat tgcgcagcga ttcggaatca gtgaatcgac
      901 agcgaataca gctatccagc ttgctgttcc cttaatactc agcgccatgg cccgcaacgc
      961 cgctcaacca caaggcgcag aaaatttgca ccaggcactc aactccgatc atgacggcag
     1021 tatttttaat gatttgatgg gccatctcag taatccccaa acggcaaacg gagctggaat
     1081 tttgggccac gtatttgcag gtcagcaggc gaacgtggaa agcaatctgg cgcaggcggc
     1141 gggaatcgat cagagctcag cgggaggctt gatggaaatg gtagccccac ttgttatggg
     1201 agcagtgggc caggcccagc agcaaaatgg acttgacgct tcgcaactct cgaacatgtt
     1261 gacagttcag cagcaacagg ccgaggccac ggcgcctgat gttatgggca tgctgggttc
     1321 aatgttggac cagaataagg atggtagcgc tatggatgat ctctggcggg tcgccggcaa
     1381 tttctttaaa tgagtctgag taataaagca ctcgtcgccg ttcggtctgc agtatgcagc
     1441 ccaggccgat tcgtcaatgt gtaatcaaac tatcaactca taggaggaac accacagtga
     1501 aaacaaaagc agtcggcatc gcagtgatgc ttacccttat cgccgcgctt atgggatgta
     1561 atcgcggtcc taatgatgaa acgctgacac aacaagtgaa ggcaaagatt agtgccgacc
     1621 cggcattagc cacacaaccg gtcaacgtct ctgtaaaaga cggagtagtt accctcgccg
     1681 gtaacgtaaa taccgcgact aataaagcca aggctgaaga tttagcaaag ggtgtcgacg
     1741 gcgttaagtc ggtaagtaac tctttgtcgg ttagaccacc aattgtgaat gccactccgc
     1801 ctccggtatc tccagacacg cagctcaaaa caagcgtcat ggcagggtta actaaatatg
     1861 gaatcaccac cgtgtctgcg gaagtggtta acggggaagt gacgctttca ggcaatattc
     1921 cgcgtgcaaa actacaggac gcaatgaaag cggcgaatga atctcatccc aaaaaagtcg
     1981 tcaataagat gactattaag tgaaggagtt gtaatcatgg aacttaccga gaagtacaag
     2041 cctgtaattg atattgctag ccagaacggc gtcaatccac aggtagccga gcaagatggt
     2101 gtcctctata tcacggctac taccaacaac ggttcagtga aacagcagat gtgggacaag
     2161 tacggggaga tagatcctga ctaccgatca ggtgatctgg ttttgaatgt tgaggtagaa
     2221 ggcgggggtt acgaagaata tacggttcag tctggagatt ctttgagtaa gatcgggagc
     2281 cactggggaa agaactggaa agagatatgg gacttaaacc gggacgtgat cggagctaat
     2341 tataatttga ttcacccggg acaaaagctg cgcataccta aataaagttg tccgaccaag
     2401 aaggggcgcg aggatatccc gcccctttca tttgccatcg atgcggcgca cgtcaactat
     2461 ttcgccgcga aaggtgcgca tcatttcctg gactatcgga ttgctctccg cagactctcg
     2521 aagcttcttt ttctcgatcc gttcgtcatc ttcccgagtt gccgggccag atgattgttc
     2581 ctgcagatct ctgacggcga ttcgaactcc ggcctcctga ccggttacct cacgacaaac
     2641 ttcccgcagt atttttatgt tctctgcctt agtgagcgta tcgcgaaaat gtcgagactc
     2701 gggagtaaac tcaacaaaga gttctccgtc ttcgatcgag gcggcgtgag cagcttcgag
     2761 cactgtaacc aacagcatct tccgttgatt ttcgagcgcc gctttgattc ggtccatgac
     2821 cgtcatctcg gagccagtcg taattccatc atcggttgtg gccggctcgt ctatggcagc
     2881 ggtcgactga accgtgacct ctgttttcat ttcaaaggct ggagcgacgg ctgccgcgct
     2941 aaatgaagat gatgccgcac ggctgccaga attgatctga cctgcgcctc ctttgcgagg
     3001 agccggcgat ccatttcccg agctggacga accgccccca ggcttccctg atgaacttcc
     3061 ctcaagagtt gccaggcgct gcagtatctc tgagatggat tcaactccac ggagttccat
     3121 caatttgacg ataccgatct caacctgata tcgcggctga gttgcagttc gaagttttgt
     3181 ttcagtttcc gccagtgaat gaaagaaacg aacaaggtcc gattccgaaa aaagatccgc
     3241 ttgccgtttg agttcgacag gttcacaaac agctgcctct aacagttctt cattgcccga
     3301 tactttggtt acgagcagat ctcggaagtg ggccaaaagg tctcgacaaa aacttcttaa
     3361 gtcgtgacct ctcatcacga tgtcatctac gaccgctatg gcgtctgcgg gtttattgtt
     3421 cgcaatgctg tcgataacgc gagtcaaaat gtcggcgccg gct
//
LOCUS       DASSFP010000200         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2132160, whole genome shotgun sequence.
ACCESSION   DASSFP010000200 DASSFP010000000
VERSION     DASSFP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132160"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VFH91_04335"
     CDS             <1..1602
                     /locus_tag="VFH91_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862245.1"
                     /translation="VKWVEGDLFQNDFGKFLPSVGFAWDPFKSGKTSIRGNYRLASDR
                     MATFLFGSFIFQNAPGNTFAAQNTSFGANGGLYRNVGPIIAALAPPSSPAALQQPAAF
                     NTTSVNVIDPDLQFPQIHEWSLSFQREIRNNVVEVNYIGKHGVHLLGGYNANQVNIFA
                     SIPGTGEANFLDAFNKVRASSSYNSPTINLLMSGNAANNGGTARFRSLNTTAISQGSV
                     AAAALVVSQRTCAADDVTAGICTNAQRGQRLNDLYGFPFLLQPFPQFTGGVNVFDSND
                     YSNYQGLQLIFRRRINDGLGFQFGYTYSLSKDNRSWDPALSGVTTGSVQSASSTPFDL
                     RDRSLNYAWSDFDRRHVFQGTYTYELPFGKGKQFASGANSVVQHIIGGWQTSGTVILM
                     SGRPFTVYSGLNTLSNVVQSTADCNGCSRDSGHLVLETGRNFWFDSATRSQFSATAPG
                     SIGNTGRNFFIAPKYFQWDASLLKKFRISERVSFDLRVDARNVLNKPSFDNPTAVLTS
                     TIFGRINDSVTNNARRIQWSGKLSF"
     gene            1632..>2534
                     /locus_tag="VFH91_04340"
     CDS             1632..>2534
                     /locus_tag="VFH91_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6862246.1"
                     /translation="MKLGCRLLLLVWLLAAPTVIFRSDSHSQAVSPSLDLVVTNARVI
                     DGSGNPWFRADVGVRDGRIVRIGRITPSEARQTIDAKGQILAPGFIDVHTHVESIYSL
                     PAAENFVRMGVTTLVTGNCGTSATNIGEFLGRTKSTPIAVNLATLIAHGSVRRQVVGL
                     DDRAPSADELKRMEDMVEQGMKDGAVGLSTGLIYVPGTYAKTDEIVALAKVAARYGGV
                     YATHMRNEGDKVADAIRESIAIGEQAGLPVEISHFKISNKKLWGQSSMTLGLVRDARA
                     RGLQVTVDQYAYTASSTSLESRLPS"
BASE COUNT          598 a          657 c          656 g          623 t
ORIGIN      
        1 gtgaagtggg ttgaaggcga cctcttccaa aatgactttg gaaaatttct accgtcagtt
       61 ggatttgcct gggacccatt taagagtggt aagacgtcta ttcgcgggaa ctatcgtctt
      121 gcttcagacc gaatggcgac cttcttgttc ggctcgttca ttttccaaaa tgctcctggc
      181 aacacctttg cagcgcagaa tacctcgttt ggcgccaacg gaggactgta ccgaaatgtt
      241 ggccctatca tagccgcttt ggcgccgcca tcttctccgg cggcattgca acagccggcc
      301 gcgttcaata ccacatcggt taatgtcatc gatccagatc tgcagtttcc gcagattcac
      361 gaatggtcat tgagttttca gcgtgagatc cggaacaacg tcgtggaagt caactacatt
      421 ggcaaacacg gtgttcactt gcttggcgga tacaacgcga accaggtaaa tatatttgcc
      481 tcgattccgg gaacaggtga ggcaaacttc ctggacgctt ttaataaggt gcgcgccagc
      541 agttcgtaca acagtccaac aatcaatctg ctcatgtcgg gtaatgctgc caataatggc
      601 ggcactgctc gattccgctc gcttaataca accgccattt cgcaaggcag tgtcgctgca
      661 gccgctttgg tggtatcgca gagaacctgc gctgctgatg atgtcaccgc cggtatctgt
      721 accaacgctc aacgtggtca acgactcaat gatctgtacg ggttcccgtt cctgctgcag
      781 ccgttcccgc aattcacggg tggtgtgaat gtttttgaca gcaacgacta ttcaaactat
      841 caaggtctgc agttgatctt caggcgccgc atcaacgacg gcttaggatt tcagtttgga
      901 tacacttact cgctctcgaa ggataatcgc tcatgggatc ctgcgctgag tggcgtaacc
      961 accgggtctg tgcagtctgc gtcgagcact ccgtttgacc tccgtgatcg cagtttgaat
     1021 tacgcctggt ccgactttga tcgaaggcac gtattccagg gtacctatac ttacgagtta
     1081 ccgtttggca agggaaagca atttgccagc ggcgccaact cagtagtgca gcacatcatc
     1141 ggcggctggc agacttccgg aacggtcatt ctcatgtccg gacgaccatt caccgtctat
     1201 tcaggtctga acactttgag caacgtggtc cagtcaactg cggactgtaa cggctgctcg
     1261 cgagacagtg gacacctggt gctggagaca ggcagaaact tctggttcga ttcggcaact
     1321 cgttctcagt tttcggcaac agcgccaggc tcaatcggaa atacgggacg caatttcttc
     1381 atcgcgccaa aatatttcca atgggatgct tcattgttga agaagttcag aatttcagaa
     1441 cgagtttcgt tcgacctgcg agtagatgca cgaaatgtgc tgaacaaacc atcgtttgat
     1501 aatcctacgg ccgtgcttac ttcgaccatt tttggccgca tcaatgacag cgtcacaaac
     1561 aatgcccgca ggattcagtg gtcaggaaag ttgagcttct agagtttttt aacagggagc
     1621 agactcgcta aatgaaactc ggttgtcgac tattgctgtt ggtgtggctg ctcgccgcac
     1681 caacagttat ttttcgttca gattcacata gccaggccgt tagtccatcg ctggacctgg
     1741 tcgttactaa tgctcgggta attgatggga gcggtaatcc gtggtttcga gcggacgtgg
     1801 gcgtgcgcga cggtcgcatt gttcgaatcg gcaggattac gccctcggaa gctcgccaga
     1861 caattgatgc gaaaggacaa atcctcgcac ccggattcat cgacgttcac actcacgtag
     1921 aatccatcta cagtttgccc gccgcagaaa atttcgtgcg catgggcgtc accacgttag
     1981 tcactggaaa ctgcgggact tccgcgacga atattggtga gttcctcgga agaacaaagt
     2041 caaccccaat cgccgtcaac cttgcaacgc tcattgcaca tggatcggtt cgtcgacaag
     2101 ttgtcgggct cgatgatcgt gcgcctagcg ctgatgagct caaacgcatg gaagacatgg
     2161 tggaacaggg gatgaaagac ggtgcagtcg gtttgtccac cggattgatt tacgttccag
     2221 gcacttacgc taaaactgat gaaatcgtag ctcttgccaa agtcgccgca aggtatggtg
     2281 gcgtttacgc gactcacatg cgaaatgaag gcgataaagt cgctgacgca attcgcgaaa
     2341 gtatcgccat cggagagcaa gctggactgc cggtcgaaat ctcgcacttc aagatcagta
     2401 ataaaaagtt gtggggccaa agctcaatga ccttgggcct cgttcgcgat gcccgcgcgc
     2461 gagggttgca agttacagtt gatcaatacg cttacaccgc aagctcaact tctctcgaat
     2521 cacgtctgcc ctcg
//
LOCUS       DASSFP010000201         6369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1687815, whole genome shotgun sequence.
ACCESSION   DASSFP010000201 DASSFP010000000
VERSION     DASSFP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687815"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFH91_04345"
     CDS             <1..253
                     /locus_tag="VFH91_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862247.1"
                     /translation="VAVTSDGGATWTLGSGLSGFRSVVAYSDGAFVAIGPSGSDISHD
                     DGKTWSPIPGPGFDTFSFVKGSNLGWGSGAQGNIGKLSL"
     gene            complement(516..1274)
                     /locus_tag="VFH91_04350"
     CDS             complement(516..1274)
                     /locus_tag="VFH91_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET6862248.1"
                     /translation="MSRSIKVTLLVLAALTIAGFLFSTSRQTVASPPSNCQNLPSETQ
                     LKGFLKAAQTAGGTVGGLFEGTRMWGAVVNRDGEICTYTTSTDDPTQVWPGSQAIAKA
                     KAYTGNAFSLDTLALSTARLYTFTQPGHSLWSLGQSNLFDPKALAAPSGGSGGKNQIA
                     GGLIFFGGGVPLYNSQGKIIGALGVSGDTSCADHEIAKRVRNLAGLNPPGGALVDDIT
                     YSSADGASPFTHPLCPNTFRNGVFIGNEQNASGY"
     gene            complement(1466..1723)
                     /locus_tag="VFH91_04355"
     CDS             complement(1466..1723)
                     /locus_tag="VFH91_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862249.1"
                     /translation="MGWDGDDLAGSMEDAAERQQSLIDERLTSSAKRREQRAKNSELE
                     SLRMSKSRIMGQLENAVNPVHRTMLERALTAIEEKLVVNLK"
     gene            2189..6097
                     /locus_tag="VFH91_04360"
     CDS             2189..6097
                     /locus_tag="VFH91_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6862250.1"
                     /translation="MARLLSKIFFRFPHDRRLDSGRRFPATMLLRRLSLVSISILVLC
                     AAAYAQSGNATVRGTVKDPQGNVVAGANVKLTNTEKNFTRTQSTNQEGAYTFNAIPPG
                     TYRLEVEAAGFKTAAASGVAALVDTSTVRDLQLEVGAVSETVDVTSGAETAINTTDAS
                     IGNSFERKRIVELPLNANNVVGLLSLQPGVTRSGYVNGGRSDQSNITLDGVDVNEQQD
                     GLDVVTDEAFASVLRVTRDSLQEFRVITTNPNAEQGRSSGAQVSLVTRSGSNQWHGSL
                     FETHRNTVTTANDFFNNAAGVERPQLLRNIFGGSVGGPIKKDRAFFFFTYEGFREATA
                     TSVVREVPLPTLGQGIVRYRTTDVFSDPGCPAGTPSGVDCLTPDEINAAYTAFNGSTP
                     GINPAGLAVLADAARRYPVNDSTVGDGLNTSGFRFNARTPTELNTYIARFDVNLTDRQ
                     TLFLRGNYQNDTVTRAVYANSDCSVPPDNIQCFPDTPPLTTWNHPKGLAIGHTWTASN
                     SIVNRFNYGLTRASFTQGGDSNENRVNFRFIYSPNSFARSLSRTTPVHNFVDDVSWIR
                     GNHTIGFGGNVRLITNNRNSLAASFDDAVINPSFYNVSGRVITDPFSNFESRNDLRDA
                     LSSVIGRYSQYSANLVYGADGSLQPVGTPTERAFATQEYEFYGQDSWRIRPNFTLNYG
                     LRWSTSTPVYEKNGLQVVPNINLTDYFNQRVASSNAGSSFNDPITFILGGKGNNAPGY
                     YKQDWNNFAPSLSFAWSPDFGKNWLGRAFGREGKSVIRGGFRMTYDRIGSQLAVNFDL
                     NNLAGFTSARNINANTFDVIPGELGPLFNGFNPQVRNLPFPGSTGPIPTSLNFPLTVP
                     SDEDQRIETSLDAGITTPYNTSFNLSYARELGRGLTFEASYVGRFARKILATRDIMQL
                     NNIVDPQSGMSFYDAMNQLVDARYANAPINSLAPIPFFENMFPFAADWWGDSSLTATQ
                     ASYAWIAPGAVGGADINDYTFVQLLWDDSPNCTTCPFGGGPAKFNNMFYQPQFGALSA
                     FSTIARSNYHALQLSLRERLRDDVTFDFNYTYGHSTDNASGLQSSTSYGTAFIVNSLY
                     PDRNYASSDFDATHIINANWVVGLPVGRGKKFLNNSSGFVDTLLGGWQLTGIFRYNSG
                     LPFQTPFDCCFWATNWNVQSNGVRVRPVQATTNKSGTPNAFADPVAAFRSFRNARPGE
                     VGDRNVLRGPSYISLDSGLYKTFKLPWEGHTLQFRWEVFNVTNTQRFDPLTISDLSLG
                     TDPFLGGNPNSDFGRYTSTQTPLNETKAGRVMQFALRYSF"
BASE COUNT         1534 a         1673 c         1512 g         1650 t
ORIGIN      
        1 tgttgctgtt accagcgacg gtggagcaac ctggacactc ggatcaggac tctcgggatt
       61 tcgttctgtt gttgcctaca gcgacggcgc gtttgttgcg attggtccaa gtggctcaga
      121 catatcacat gatgatggca agacatggag tcccattcct ggaccaggct ttgacacctt
      181 cagttttgtt aaaggcagta accttggttg ggggtcaggt gcgcaaggta atattggtaa
      241 gttaagtttg tgaggaacta ttttatttta aaagcagaga tcagtatccg tctcgggata
      301 gcctcaggtg agtcccatta atggtcaatt atgatttgat atttgagaat tgttaactgt
      361 gcgcgccatt ccacatcgct gcgggcgcct acaacattgg tcataattaa taattctcaa
      421 atatcaaatc ataattgacc cttaatgggg actcactcac aacgttaccg cgttacgata
      481 ctgacctctc ccatcggtct aacttctaac tgctcttaat atcccgaagc gttttgctcg
      541 ttgccgatga atactccatt tctgaaggta ttgggacaaa gtggatgagt aaacggcgaa
      601 gcaccatccg ccgaagagta cgtgatgtcg tctaccaggg cgcctcccgg cggattcaat
      661 ccagccaggt tacgaactct ctttgctatc tcatgatcag cacatgaggt gtcaccgcta
      721 actccgagtg caccaatgat ctttccctga gagttgtata atggcactcc gccaccaaaa
      781 aagattaacc cccctgcaat ctgattctta ccacctgatc caccactcgg agctgcgagc
      841 gcttttggat caaaaagatt tgattggccc aggctccaaa gagagtgacc tggctgcgtg
      901 aaggtataga gtctcgcagt tgataaggcc agagtatcaa gactaaacgc gttgccagtg
      961 taggccttag ctttggcaat cgcctgactg ccgggccaca cctgtgttgg atcatcggtc
     1021 gaagttgtat aggtacagat ttcaccgtcc cgattcacga ctgctcccca catccttgtt
     1081 ccttcgaata gaccaccaac ggtacctcct gcagtttggg ccgccttaag aaaacccttc
     1141 agttgagttt cacttggaag attctggcag ttcgaaggtg gactcgcgac tgtctgtcgt
     1201 gaggtcgaga acagaaaacc ggcaatcgtc aatgcagcga gtaccagcaa agtaacctta
     1261 atcgatctac tcatcggact ctcctttcaa tttggggaca attcaatcga ggcgaatggg
     1321 tcgaaagaca gttttgccct gatctggacc atgtcaatac ggagcccaga cacctgcttg
     1381 actcacaata attaggcggt ggaaaaatac ggcggatgtt ggttcaagtc aatagattgg
     1441 caacgttaag gaggacgatt actcgtcact taagattaac cacgagcttt tcctcgattg
     1501 ccgttaatgc tcgttccaac atcgtgcggt gaaccggatt tacggcattt tcaagttggc
     1561 ccataatgcg tgacttcgac atcctcaatg attccaactc tgagttttta gctcgttgtt
     1621 cacgcctctt agccgacgac gttagtcgtt cgtcgataag actctgctgc cgttcggctg
     1681 catcttccat gcttccagcg aggtcatctc catcccagcc cataatagaa ctccttctcg
     1741 gactacgatc gacatctagt cgtcttaacc agaactccta aaaatgccca ttttcgtcca
     1801 cagatctaaa tatacgtagc aagttacaaa atctcgcagt tcagaaacag caagcccact
     1861 caggtttttg gacaagcttt tcggataaat ggagttaagt aactctactt tgcgccaata
     1921 catgcattaa atcaacttga atcaacttgg tacaaaaagt gcgtcgttct aaaagcgtca
     1981 gtgtctcccc cacacgctcg ggagaagcgg gtgcttattc acgttcttga agctcttgca
     2041 agaatcacac gccaaccggg ccaatttctc cgcttacgtt ccctgaaatc aagcggaacg
     2101 aagtgaacgt caccccgatc aaaccagaaa attaaacttc gaattgccct gtccactcat
     2161 tgaatgacag gtcaaaggga ggcttgtaat ggcgagatta ctttcgaaaa tcttcttcag
     2221 atttcctcat gaccgccggc ttgactctgg acggcggttc ccagcaacca tgcttcttag
     2281 acgtctgtct ctagtatcaa tttctattct ggtcctttgt gctgcggcat atgcgcagtc
     2341 aggaaacgct acagtccgcg gaactgtcaa agatccgcaa ggtaatgtag ttgcgggtgc
     2401 gaacgtaaag ttgactaaca cggagaaaaa ttttactcgc actcaatcaa caaatcagga
     2461 aggcgcctac acctttaatg caattccacc gggcacctat agactcgagg tcgaagccgc
     2521 tggttttaaa actgcggcgg catcaggagt tgcagccctc gtcgacacat ccacagtccg
     2581 cgacctgcaa cttgaagttg gcgccgtttc ggaaacggta gatgtgacct caggtgcgga
     2641 aactgccatc aataccactg atgcgagtat tggcaactct ttcgaaagaa aacgaatagt
     2701 cgaattaccc cttaatgcca ataacgttgt tggtctgtta tcgctccagc cgggcgttac
     2761 caggagtggt tatgtgaacg gtggtcgatc ggaccagtca aacatcactc tcgacggtgt
     2821 agacgttaac gaacagcagg acggactcga tgtcgtaact gacgaagcgt tcgcaagtgt
     2881 tctgagagtg actcgcgatt cgcttcagga gtttcgcgta atcacgacaa acccgaatgc
     2941 tgagcaggga cggtcatccg gcgctcaggt ttcactcgta acgcgatcag gatcaaatca
     3001 atggcacgga tccttgttcg aaacacatag aaacaccgtt actactgcga acgacttttt
     3061 caataacgcc gcaggcgtcg agcgaccgca attactaaga aatattttcg gcggttccgt
     3121 tggtggtcca atcaagaagg atcgcgcctt cttcttcttc acttacgaag gatttcgcga
     3181 agcaactgct acgtcagtag ttcgtgaagt gcctctgccg acgctgggcc agggtatagt
     3241 tcgttatcga actacagacg tcttcagcga tcccggatgt cccgctggta cgccttccgg
     3301 tgtcgactgt ctaacgcccg atgagatcaa cgctgcttat actgcgttca acggttccac
     3361 cccgggcatt aacccagctg gcctcgctgt ccttgctgac gccgcaagac gttacccggt
     3421 gaacgattca accgtaggtg acggcctaaa cacgagcggc ttccgtttca acgctcggac
     3481 ccctactgag ctcaacacct acatcgcccg attcgacgtc aacttgacgg atcgtcagac
     3541 cctgttcctg agaggcaact accagaacga tacggttacc cgagccgtct acgctaattc
     3601 agactgcagc gtcccccccg acaacatcca gtgttttccg gacactcctc cattaacaac
     3661 ctggaaccat ccaaagggac tcgcgattgg acacacatgg actgcatcaa attccattgt
     3721 taaccgtttc aactacggtt tgacgcgagc ctcgtttacc cagggtggtg attccaatga
     3781 gaatcgcgtc aacttccgct ttatctattc accgaattcg ttcgctcgta gtttgagtag
     3841 aacgacgcca gtgcataact ttgttgacga cgtttcctgg atacgtggca atcacacgat
     3901 cggttttggt ggaaacgttc ggttgattac aaacaaccgc aattcgcttg cagcttcatt
     3961 tgacgatgcg gtcatcaatc cctcgtttta caacgtatcg gggagggtga tcacggatcc
     4021 cttctcaaac ttcgagagtc ggaacgacct tcgggatgcg ctctcatcag tgattggccg
     4081 ttactcgcag tatagtgcga acctcgttta cggtgctgac ggctcgctgc aacccgtagg
     4141 aacacctacc gagcgtgctt tcgcgaccca ggagtacgaa ttctatggac aggattcgtg
     4201 gcgcatcaga ccaaacttca cattgaatta tggacttcgc tggtccactt caacgccggt
     4261 ctatgaaaag aatggcctgc aggtcgtgcc caacataaac ctcaccgatt acttcaacca
     4321 acgcgttgcc agctcaaacg ccggctcttc attcaacgat ccaatcacct tcatcctggg
     4381 cggcaaaggt aataacgctc cgggctatta caaacaagac tggaacaatt tcgctccatc
     4441 actttccttt gcctggtcac cggactttgg caagaactgg cttggaagag cgtttggtcg
     4501 tgaaggcaag agcgtcatac gtggtggttt ccgcatgacg tacgatcgaa ttggcagtca
     4561 actcgccgtt aatttcgacc ttaacaacct ggccggtttt acttccgccc gtaacatcaa
     4621 cgcgaacact ttcgatgtga tccccggcga actcggtcca ttgttcaacg gattcaatcc
     4681 tcaggttcgc aatcttccct tccctggctc aactggacca attccaactt ctctcaattt
     4741 cccgttaacc gttccttcgg atgaagatca acgaattgag acatccttag atgcgggtat
     4801 caccacgcca tacaacacca gcttcaactt gtcgtatgcg cgtgaactcg gaagagggtt
     4861 aacgttcgag gccagctatg ttggacgttt tgctcgaaaa atattggcca cacgcgatat
     4921 tatgcagctc aacaacattg ttgacccgca gtcagggatg agcttttacg acgcgatgaa
     4981 tcaactcgtt gatgctcgtt atgcaaatgc gcccatcaac tcgcttgcgc ctatcccgtt
     5041 cttcgagaac atgttcccgt ttgcagctga ttggtggggt gacagctcac tgacagcaac
     5101 ccaggccagt tatgcctgga tcgctccggg agctgtcggc ggtgccgaca ttaatgacta
     5161 cacattcgtg cagttgctct gggacgatag tccaaactgc acaacttgcc cgtttggtgg
     5221 tggtcctgcg aagttcaaca acatgttcta tcaacctcaa ttcggtgcgt tgtcagcctt
     5281 ctcgactata gcgcgttcga attaccacgc tttgcaactg agcttgcgtg aacggttgcg
     5341 agatgatgtc acgttcgact tcaactacac ctacggacat tcaaccgata acgcctctgg
     5401 actacaaagc tcgaccagtt acggaactgc tttcatcgtt aactctctgt atccggatcg
     5461 caactacgcc agctcagatt ttgatgctac ccatatcatc aacgcgaact gggtggtcgg
     5521 attacctgtc ggacgcggaa agaagtttct caacaactcg agtgggtttg tagacacttt
     5581 gctgggagga tggcagctga ccggtatttt ccgctacaac agtggtcttc cgttccagac
     5641 tccatttgat tgctgcttct gggccacgaa ctggaacgtg caatcgaacg gcgtgagggt
     5701 ccgaccggta caagcgacga cgaataaatc tggaacacca aacgcctttg cggacccggt
     5761 tgcagcattt cgcagcttcc gtaacgcgag acctggcgag gttggtgatc gtaatgtact
     5821 acgtggtcca agctacatct cgttggactc gggcctctac aagacgttca aacttccctg
     5881 ggaaggtcat actctgcaat ttcgctggga agtcttcaat gtgaccaata ctcagcggtt
     5941 cgatccgttg acgatttcag atctgagcct gggaacggat ccatttcttg gaggaaatcc
     6001 gaactccgat tttggacgtt acacctcgac tcaaactccc ttgaacgaaa ctaaagccgg
     6061 acgagtaatg cagttcgcgt tgcggtactc gttctaagct aatttaagag cggtgatctt
     6121 cgggtcggag tcaaaatggc tccgaccttt ttttgtccct aagatgttgt ccctggtctg
     6181 tgctttgtgc tttgtgcttt gtgctttgag ctttgagctt tgtgctttgg tgctttggtg
     6241 ctttgtactt tgtctttggt gctttgtact atttccgggt aattgaggcc tggtctttga
     6301 tgtcgggtgg tagccgaggt ttttggagtg cggcggcccg gcgccgcttt gttaacacag
     6361 cttctcgac
//
LOCUS       DASSFP010000202         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2132686, whole genome shotgun sequence.
ACCESSION   DASSFP010000202 DASSFP010000000
VERSION     DASSFP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132686"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="VFH91_04365"
     CDS             complement(<1..1577)
                     /locus_tag="VFH91_04365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6862251.1"
                     /translation="MTRLARVLLLFSMVLAFLAAPIMAQTTGSISGEVKDEKQAVVTN
                     ATVIVRNVKTNDTRTAQTDTEGRYRFTGMPVGDYEVTVEATGFSKYVQSGISLLLGQP
                     ATVDVELRPGGVTEVVNIIENASILNTTNTEVGTRFDNRRLAELPIATNRNVYNIALS
                     APGVSQLQSNQSQFTNGINYSSNGGRLRSNNFLLDGQDNNDFGVGGAVVQLNNPDLIQ
                     EVRLVTNQFSAEYGRNSSSVFNAITKSGSNEFHGSGFWFHNDNHLNACSNLNKNGGFC
                     NKNATDAAHQIAPFRIENQAGLTFGGPLYLPHFGEGGPTYSSGKDKTFFFFALQRWWD
                     RQLGQGVTIQGVPTDAGRQILQTPAGSRAQVQALLAFLPPAQAPNGQTRPFTINGQTF
                     SVPLGSLTGSSSFVFNDWQQSIRLDHRLNDNHNITGRYIYQDQDTVGAGQSTPSGYES
                     ANVSRNQGVNIALNSILSSRMVNELRLSYLRAASATVAADLRAEAIPSIQITDLGMTG
                     LNAGATRTAIGLAANLP"
     gene            1872..2837
                     /locus_tag="VFH91_04370"
     CDS             1872..2837
                     /locus_tag="VFH91_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET6862252.1"
                     /translation="MSTSTKKHSIVFLERNTFSVEFRKPAFDHDWVEFNETRSDEIVG
                     RLRNATIVICNKLPLRRETLSQLPGLQLIAVAATGVDNVDLEYCRSHQIAVINTRGYA
                     VHSLPEHALMLMLALRRNLIAYRNDINRGAWNQAKQFCLLDHPINDLKDSTIGVVGFG
                     RLGQSMAQLATAVGMKVLVAERKEATGTRAERTPFVEVLRQSNIVSLHCPLTAETENL
                     IGSDELNLMRRDSILINTARGALVDEHALLQALRTNQIAGAGIDVLRQEPPRDTNPLL
                     EARLPNLIITPHNAWASQQAMQNLADQLVDNLEAFVRGEPRNLVF"
     gene            complement(2913..>3253)
                     /locus_tag="VFH91_04375"
     CDS             complement(2913..>3253)
                     /locus_tag="VFH91_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862253.1"
                     /translation="TGHGFQRVQQFATSKTVTNTDFREEKFRVFMSENEAAIIEGAAQ
                     SPEPTPAASTEEISYHAVEKDGVVTAIWEMQGRKHLRTIDPRSRQGQDILRLYTTEHH
                     DEIAGEAAAS"
BASE COUNT          802 a          793 c          838 g          820 t
ORIGIN      
        1 tgaggcaggt ttgccgcgag gcctatagct gtacgagttg cgccggcatt caatccagtc
       61 ataccgaggt ctgtaatctg aatggacgga atcgcctctg cacgcaagtc agcagcaacg
      121 gtagcactgg cagctcgcaa gtacgagagt ctcagctcgt tgaccatcct ggagctgagg
      181 atgctattga gagcgatgtt cacgccttga ttacgcgaaa cgttcgctga ttcatagccg
      241 gaaggtgtcg actgacctgc tccgaccgta tcctgatcct gatagatgta gcggccggta
      301 atattgtgat tgtcgttcaa tcgatggtcc aaccgaatcg actgttgcca atcgttaaag
      361 acgaaggagc ttgaaccggt gagtgaaccc aaaggaactg agaaggtctg tccgttaatc
      421 gtaaatggcc tggtctgacc attcggcgcc tgcgctggag gcagaaaagc aagtagtgcc
      481 tgaacctgag cacgactgcc cgccggagtt tgaaggatct gacgtccagc atcagtggga
      541 acaccctgaa tggtgacacc ctgaccaagt tgtcgatccc accatcgttg cagcgcaaag
      601 aagaaaaacg tcttgtcctt gccgcttgaa tacgttgggc caccttcacc gaaatgcggc
      661 aggtaaagtg gaccaccaaa agttagtccg gcctgatttt caatacggaa cggagcaatt
      721 tggtgagcgg catcggtagc gttcttattg caaaagccac cattcttgtt caggttgcta
      781 catgcattca aatggttgtc gttatggaac cagaaacctg aaccatgaaa ctcgtttgag
      841 ccactcttgg taatggcgtt gaatacagaa ctactgttgc gaccgtactc ggcgctgaac
      901 tggttagtca ccagtcgcac ttcctggatg agatccggat tgttcaactg gaccacggcg
      961 ccgccaacac cgaagtcgtt gttatcctga ccgtccagca gaaaattatt ggagcgcaga
     1021 cgtccgccgt tcgatgagta gttaatacca ttcgtgaact gtgattgatt cgattgaagt
     1081 tgacttacac cgggagccga cagtgcgatg ttataaacat ttcgattcgt agcgattggg
     1141 agttctgcca gtcgtcgatt gtcgaaacgc gtgccaacct ctgtgttagt agtgtttaaa
     1201 atcgaagcgt tctcaataat gtttactacc tcggtaactc ctcccggtct cagctcaaca
     1261 tccacggtcg ctggttgccc aagtaataag ctgattcccg attgcacata ttttgagaag
     1321 cccgttgcct cgacggtgac ttcataatcg ccaaccggca taccggtaaa gcgataccgt
     1381 ccttccgtat cggtctgagc ggtgcgcgta tcattggttt ttacgtttcg cacgattacc
     1441 gtggcgttgg tgacaactgc ttgcttctca tcttttactt cgccagagat tgagccagtc
     1501 gtctgtgcca tgattggagc tgccagaaac gcgagcacca tagagaatag gagtaagact
     1561 cttgcaagcc tggtcataag ttatctcctt gtacacaggg cgttggtgaa aacgctcagg
     1621 ggtggtttgg atccttagag atgaatcccg ctgttcttgt ttgggcaatg cccgcttaga
     1681 tctaggcaag caacattcca actaattgta taaagcgaac aaaagaacta aacaaagcag
     1741 gaacaaggct tattagatcg gactgcagct atttgaatac aaattctgaa tattggatgc
     1801 ggtattgttc aaatctgtga attgatccat tctcgtggag agaacaccaa cctcttaaag
     1861 acggcgaaac tatgtcgacc agtactaaaa aacattcgat tgtcttcctt gaacgaaaca
     1921 ccttcagtgt tgagtttcgc aagccagcct tcgatcacga ctgggttgaa ttcaacgaga
     1981 cccgctcaga cgaaattgtc ggaaggctgc gaaatgccac aatcgtgatc tgcaacaagc
     2041 ttcctctgcg aagagaaact ctgtcgcaac taccagggct gcagctcatc gcagttgccg
     2101 caactggtgt cgataatgtg gatctcgaat attgccggtc gcaccagatt gccgtgatca
     2161 atactcgcgg atacgcagtc cactctcttc ctgaacacgc cttgatgctg atgctggcac
     2221 tgcgtcgcaa cttaattgct tatcgtaatg acattaatcg cggagcatgg aatcaggcca
     2281 aacagttctg cttgttggac cacccgatca atgatttgaa ggactcgaca attggtgtcg
     2341 taggttttgg aaggttgggc cagtcaatgg cccaactggc gacggcggtt ggcatgaagg
     2401 tgctggttgc agaacgtaag gaagcgaccg gcacgagggc cgaacgcact ccctttgttg
     2461 aggtgttaag acagagcaac atcgtatcgt tgcattgtcc gcttactgct gagacggaaa
     2521 atctaatagg gagtgacgag ctaaatctga tgaggaggga ttcgattctc atcaacaccg
     2581 cgcgcggcgc gttggtagat gagcacgcgt tacttcaggc actacgtaca aaccagatcg
     2641 ccggagcggg cattgatgtt ttaagacaag agccaccgcg agacactaat cctctattgg
     2701 aagcgcggct accaaatctc ataataacgc cacacaatgc ctgggcgagc caacaggcaa
     2761 tgcaaaacct ggcggatcaa ctggtcgata acctcgaggc gtttgtacgt ggtgagccgc
     2821 gtaatctggt tttttgagac tcagatattg cggctaagtc gggcgtcgtt tcttttgcgg
     2881 cttcggccta ctgagtcaac caccgttgtc gtttaggagg cggcggcttc tccagcgatt
     2941 tcatcgtgat gttcggtggt gtaaaggcga agaatatctt gtccttgacg tgagcgagga
     3001 tcgattgttc gtaggtgctt acgtccttgc atttcccaaa ttgcagtcac cactccatct
     3061 ttctcaaccg cgtgataaga aatctcctca gtgctagctg ccggcgtcgg ctccggggac
     3121 tgggccgcgc cctcaattat tgctgcttca ttctcagaca taaaaactct gaatttctcc
     3181 tctcgaaaat ctgtgtttgt tactgttttg gatgttgcga attgttgcac acgctgaaaa
     3241 ccgtgtccag ttg
//
LOCUS       DASSFP010000203         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1383514, whole genome shotgun sequence.
ACCESSION   DASSFP010000203 DASSFP010000000
VERSION     DASSFP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383514"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VFH91_04380"
     CDS             <1..1524
                     /locus_tag="VFH91_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862254.1"
                     /translation="AVRLFGWTAEEVIGKHVNEWQFVFADDVDAVELVTNRQRQGFEV
                     HGLLRNRNYTKDGAVLFCEWYNSVLHDEEGNLISVLSLVLDVTAKKLAEEERSALLLR
                     ERDARKQAEEADRLKDEFLATLSHELRTPLTSILGWASMIRSGEVEGATANRAIETIE
                     RNARSQARLIDDLLDVSRIITGNLRLDLHPLHIGPIVEAALDALRPTASAKGIELVTD
                     IVPEDCLVRGDTNRLRQIIWNLLSNALKFTPRGGSVKIILERGQTSAQLTVSDTGEGI
                     SAEFLPFVFDRFRQAEGSISRKQGGLGLGLAVVRHLAELHGGNVSAASEGVGKGSTFT
                     VDLPLAAERRDPARAEERRLENERRKRLRQGLTRLDGIHVLLVEDDDDSRNLLTMMLK
                     RQGAEVTAAISAAQAFRVLTQPTNSRDSVRGLPLKRPDIVISDIGMPEEDGYELLEKL
                     RRLSESEGGLIPAIAVTGYATRKDRERALEAGYQVHMAKPIEQTELLLAIATLVRKK"
     gene            1613..>3322
                     /locus_tag="VFH91_04385"
     CDS             1613..>3322
                     /locus_tag="VFH91_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HET6862255.1"
                     /translation="MRQKIFLIPVILLLVLPVFAQQNDPSVLTVDSLFSYRTRSLGPV
                     RWQQDGSGYLALEPSATKKEAVDLVRYDINSGERSIKVSAEKLVPPNSTEPLTIEEFT
                     LTPDEQKVLIFTNSARVWRSNTRGDYWVLDFATNALRKLGGPEAKPSSLMFAKFSPDN
                     QRVGYVRDNNVYVENLVGQPNIVQLTSDGSRYIVNGTFDWVYEEELFCRDGFRWSPDG
                     KQIAYWQLNSEGVKEMTLINNTAALYPVITTFPYPKAGETNSAARVGVVDTAGGATRW
                     FQLPGDPRNNYLPRMEWASNSDEVVIQQLNRLQNTDVVMLGDPRTGQVRPVLTEKEDT
                     WVDIAWGDIDWDKTGLARGDVEWIDGGKRFLWASERDGWRHLYSVSRDGRDIKVVTPG
                     AYDVISVEKVDPQNGWIYFMASPSNATQRYLFRVRIDGTGKEERLTPAADAGTHSYMI
                     SPRGDVAIHTYSSFNRVPVTDVVRLPQHSIVRTLIDNKDTQDRLAKLKQTPTEFFKVG
                     VTDGAQLDGWMIKPPNFDPQKKYPVLFYVYGEPWGQTVLDSWGGRNRLWHGMLAQMGY
                     IVLS"
BASE COUNT          840 a          786 c          897 g          799 t
ORIGIN      
        1 gccgttcgac tgtttggctg gactgctgaa gaagttatcg gtaaacacgt gaacgagtgg
       61 cagtttgtct tcgccgacga cgttgatgct gtcgagcttg tcaccaatcg ccaacgtcag
      121 ggatttgaag tgcacggcct gctccgaaat cgaaactaca caaaagatgg tgcagtgctt
      181 ttctgcgaat ggtacaactc agtattgcat gacgaagaag gaaacctcat ttctgtcttg
      241 tcgttggtct tagatgtaac cgcgaagaaa ctggccgaag aagaacgttc ggccttactg
      301 ttgcgagagc gcgacgctcg caagcaagct gaggaagcag atcgactaaa agacgagttt
      361 ctggccacgt tgtcgcatga actgcgaaca cccctaacct cgattctcgg ctgggcgtcg
      421 atgattcgaa gtggcgaggt ggagggtgcg accgcaaatc gcgccattga gacaatcgag
      481 cgtaacgcca gatcgcaagc tcgcctgatt gacgacctgt tggacgtttc ccgaattatc
      541 actggaaatc tccgcctcga cctccatccc ttgcatattg gtccaattgt ggaagccgcg
      601 cttgatgcct tgcggccaac cgcttctgca aaaggaatcg agttggtgac ggatattgtt
      661 cccgaagatt gtctcgttcg cggggatact aaccgtctgc gccagatcat ttggaacctt
      721 ctctcgaacg cacttaagtt cacgccgcga ggtggctcgg taaagatcat actggaacga
      781 ggtcaaacat cagcacaact gacggtgagt gatacgggcg aaggaatatc agctgaattc
      841 ctgccctttg ttttcgatcg ctttcgtcag gccgaaggat caatttccag aaagcaaggt
      901 ggactcggac tgggtttagc tgttgtacgt cacctggccg aattgcacgg tggaaatgta
      961 agtgctgcga gtgaaggagt cggaaaaggg tcaaccttta cagtggattt gcctttagct
     1021 gcggagcggc gagatccagc gcgtgccgag gagcgccgtc ttgagaatga gcgtcgcaaa
     1081 cgactgagac aaggtctcac ccgactcgat ggtatacacg ttttgttggt agaggacgat
     1141 gacgactcgc gtaatctact gacgatgatg ttaaaacgtc agggggccga agttacagcg
     1201 gcaatatctg ccgcccaggc gttcagggtt ttgacgcaac cgacaaatag tcgagattca
     1261 gttaggggac ttcctctcaa gcgtccggat atagtaatca gcgatattgg aatgcctgaa
     1321 gaggatggtt acgaattgct cgagaagttg cgtagacttt ctgagagtga aggcggacta
     1381 attccggcaa tcgctgtaac tggatatgca acgcgcaaag atcgcgagcg agcgttagaa
     1441 gccggatatc aggttcatat ggcgaagcct attgaacaaa cagagctact gttggcgatc
     1501 gccaccctgg tacggaaaaa gtaagtcctt gaaatgttat ttaagtagta ctgcttttgt
     1561 gttgatgttt tatgtctatg cacagaaaga ctttaatcag gtacatcgaa taatgagaca
     1621 aaaaatattt ctgataccgg tcatccttct tttggttttg cctgtatttg cacaacagaa
     1681 cgatccgtcc gtactgacgg tcgattcatt attcagttat agaactcgtt cacttgggcc
     1741 tgtacgctgg caacaagacg gcagtggata tctggctctc gaaccgtcgg ctacaaagaa
     1801 ggaggcggtc gatctcgttc gatatgacat taatagcggc gagcggtcga tcaaagtatc
     1861 ggcggagaaa cttgtgccgc ctaattccac tgagccactg acgattgagg agttcaccct
     1921 tacaccagac gagcagaagg tattgatatt tacaaattcc gctcgagttt ggcgtagtaa
     1981 tactcgcggt gactattggg tcctggattt cgcaacaaac gcgctccgca aacttggtgg
     2041 accggaagca aaaccttcct ccttgatgtt tgccaaattc tctcccgata accagcgtgt
     2101 cggttatgtg cgagacaaca acgtctatgt cgaaaacctt gtgggccagc ccaacatagt
     2161 gcaactcaca agtgatggtt ctcgctacat cgtcaatggg acttttgatt gggtatacga
     2221 agaggagctc ttttgccgtg acgggtttcg ctggagtccg gatgggaaac agattgccta
     2281 ctggcagctc aactccgaag gcgtcaaaga gatgacgctg attaacaaca cggcagcact
     2341 ctatcccgtc attaccacct ttccatatcc aaaagctggc gagacaaatt ctgcggctcg
     2401 cgtcggagta gtcgatacgg ctggcggtgc aactcgatgg tttcagcttc ccggtgatcc
     2461 ccgcaacaac tacctacccc ggatggaatg ggcctccaac tctgatgaag tagtaatcca
     2521 gcagttaaat cgcctgcaaa acactgatgt ggtaatgctt ggtgacccac gcacaggtca
     2581 agtgcgtccg gtgctaacgg aaaaagaaga cacctgggtt gatattgcgt ggggcgacat
     2641 cgattgggat aaaacgggcc ttgcgcgtgg agatgttgaa tggatcgacg gtggtaagcg
     2701 gtttttatgg gccagcgaac gagacggctg gcgccatctc tattcagtgt cccgggacgg
     2761 acgcgatata aaggttgtta ctcccggagc gtatgacgtc atcagtgttg aaaaggtcga
     2821 tcctcaaaat ggttggatat acttcatggc ctctccatcc aatgcgacgc agagatatct
     2881 gtttcgggtg cggatcgacg gaaccggaaa agaagaacgt ctgactccgg cagctgacgc
     2941 cggtacgcat tcgtatatga tttcccctcg aggcgacgtc gcaatacata cttactccag
     3001 cttcaaccga gttcccgtaa cagatgtcgt tcgtctgcca cagcattcga ttgttcgtac
     3061 tctgatagat aacaaagaca cacaggatcg tcttgccaaa ctcaaacaga cgccgacaga
     3121 gttcttcaaa gtcggtgtca ccgatggagc gcaattggac ggctggatga ttaagcctcc
     3181 gaactttgat ccccagaaaa aatatccggt ccttttttat gtttatggcg aaccatgggg
     3241 acaaaccgtg ctcgatagct ggggtggtcg aaaccgtttg tggcatggaa tgctggcgca
     3301 aatgggttac atcgttctga gc
//
LOCUS       DASSFP010000204         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_204596, whole genome shotgun sequence.
ACCESSION   DASSFP010000204 DASSFP010000000
VERSION     DASSFP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204596"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VFH91_04390"
     CDS             complement(<1..1006)
                     /locus_tag="VFH91_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6862256.1"
                     /translation="MKTQSIALATLLCVVLNCLSAVSIAMSQTPAPFNGPKKEIYHSG
                     WIDLNKNGRMDVYENPKADIEKRVSDLLSQMTLEEKTCQTATLYGYGRVLKDELPTPD
                     WKNQVWKDGIANIDEHLNGWGPGSKSVYATDMEKHVWAMNETQRFFIEDTRLGIPTDF
                     TNEGLRGVAAPIATSFPNQLGLGHTWDPELVRMVGRITGTEARALGYTNIYAPTLDLE
                     RDQRWGRTEDVYGEDPYLASRLGVEMVKGLQENYAVASTVKHFAVYSVAKGAREGQAR
                     TDPQVTPREVENILLPPFEAAFKAGVLGVMSSYNDYDSIPVTGSDYWLTKRLRNGFGF
                     K"
     gene            complement(1037..2041)
                     /locus_tag="VFH91_04395"
     CDS             complement(1037..2041)
                     /locus_tag="VFH91_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6862257.1"
                     /translation="MSKQLLLLTILLLSFSSTKARTLDVYFIDVEGGAATLIVMPDGE
                     SLLVDSGFPLQRDAIRIIHVVRDVAKLKQIDHYITTHWHRDHVGGIPMVAKVIKVKNF
                     YDHGLPTAPAPDITPGYIELYRQASRNNSVALKAGDALSLRDTSVQIRINAANGVVIG
                     EPANSPQIKSCGTDFKPIPEDRTDNANSLGFLMTYGQFKFYNGGDLTWNVENKLVCPE
                     NLVGAVDVFQVNHHGVDNSNNPALVRALNPRVAIIGNGPRKGGDARTFATLKEVPEVA
                     IYQLHRNVRTNANAPAQYVANDDELCHGEFIKLSVAPGGKSYTVTVPAKKISRQYQVR
                     "
     gene            complement(2181..>2439)
                     /locus_tag="VFH91_04400"
     CDS             complement(2181..>2439)
                     /locus_tag="VFH91_04400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET6862258.1"
                     /translation="ESFIGGPLALVQDGDMIELDVESRKLEMRVPDEELNERRSRWKP
                     RPPQYQRGFGALYAAHVTQANKGCDFDFLEAGPVTPEPEIH"
BASE COUNT          573 a          619 c          592 g          655 t
ORIGIN      
        1 ctttgaagcc aaacccgttt cttaacctct tagttaacca gtaatcgctt ccggtaaccg
       61 gaatactgtc gtagtcgtta taagagctca tcacacccag tacgccagcc ttaaatgctg
      121 cttcaaacgg aggcagtaag atgttttcta cttcgcgagg agtaacttga ggatcggtgc
      181 gcgcctgacc ttcgcgagca cctttggcaa cgctgtagac cgcgaagtgt tttacggtcg
      241 aagcaactgc gtagttttcc tgtaaacctt tgaccatttc gacgccgagc cgcgacgcca
      301 gataaggatc ctcgccataa acatcttctg ttcgtcccca acgctggtcc cgttcaaggt
      361 ccaaggtggg cgcatagata ttcgtgtacc cgagagcacg cgcttctgtt ccggttattc
      421 tgcccaccat gcgcacaagc tccgggtccc atgtgtggcc caggccaagc tgattcggaa
      481 aactcgtggc gattggagcc gcaactccac gtaatccttc gttagtaaaa tcggtgggaa
      541 tgcccaatct tgtgtcttca ataaagaagc gctgcgtctc gttcatggcc cagacatgtt
      601 tctccatgtc ggtcgcgtag acgcttttcg agcctggtcc ccagccattg agatgttcgt
      661 cgatgtttgc aataccatct ttccaaacct ggtttttcca gtcgggagtc ggcaattcat
      721 cctttaatac acggccatag ccgtagagag tcgcggtttg acaagtcttc tcctcgagcg
      781 tcatttgcga cagtagatct gaaacgcgtt tctcaatatc ggctttagga ttctcgtaga
      841 catccatgcg accattcttg ttcagatcta tccagccaga gtgataaatc tccttctttg
      901 gtccattgaa tggcgcgggt gtttggctca tcgctattga gacggctgaa agacagttga
      961 gtaccacaca caacaaggtt gcgagcgcaa ttgactgagt tttcatgttt ttatctacgt
     1021 tgaaagcgac tagtttttag cggacttggt actgtcgact gatcttctta gccggaacag
     1081 tcactgtgta gctcttgcct cccggtgcaa cggaaagttt gatgaactct ccatgacaca
     1141 actcgtcgtc gttcgccaca tattgcgcag gggcattcgc gtttgtcctg acgttgcgat
     1201 gaagttgata aatggcaact tcaggaactt ctttcagagt tgcaaatgtg cgggcgtcgc
     1261 cgcccttgcg tggaccattg ccaatgattg cgaccctggg attcaaagcc cgcacaagag
     1321 ctggattgtt actgttgtcc acaccgtgat gatttacctg gaagacatca acggctccga
     1381 cgaggttttc ggggcacacg agtttattct cgacattcca ggtcaagtcg ccgccgttgt
     1441 agaacttgaa ctgaccataa gtcattagaa aaccaaggct gttggcatta tcggttcgat
     1501 cttctggaat aggcttgaaa tccgtaccgc aactcttgat ctggggcgag ttagctggct
     1561 ctccgatcac aactccattt gcagcattga ttcgtatctg aaccgacgtg tcacgcaaac
     1621 tgagtgcatc cccggctttc agcgctaccg aattgtttct tgaggcttga cgatagagct
     1681 cgatgtatcc cggggtaata tccggagcgg gcgccgttgg gagaccatga tcgtaaaagt
     1741 tcttaacctt aattactttg gcaaccatgg gaattcctcc gacatgatcg cgatgccagt
     1801 gcgtagtgat gtagtgatcg atctgcttga gcttcgcaac atcgcgaacc acatgaataa
     1861 ttcggattgc atctcgttgt agtggaaatc ctgagtcgac caacagcgac tcgccgtctg
     1921 gcataacgat tagagttgcg gcgccgcctt cgacatcgat gaaatagaca tccagggtcc
     1981 tcgccttagt ggaagaaaag gagagaagaa ggattgtcag aaggagaagc tgctttgaca
     2041 tagttgctcc attaaaccaa gtatttttca ttggccattt ctcatttctc attttccatt
     2101 tgggagcatt tttggtttct cctggttcgc aaatgaaaaa tgagaaatga aaaatgaaca
     2161 atgagaaata gcaggttttc ctaatgaatc tctggctcag gggtgacggg tccggcttcg
     2221 agaaagtcaa agtcgcaacc tttattcgcc tgcgtcacat gcgcggcata cagtgcgcca
     2281 aatcctctct gatactgagg tggtcggggt ttccatcgcg atcgtcgttc attcaactcc
     2341 tcatcgggaa cacgcatctc gagcttgcgc gactcaacat ccaactcgat catatcgcca
     2401 tcttgaacca gcgctaatgg tcctccgata aacgactcg
//
LOCUS       DASSFP010000205         6426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_49493, whole genome shotgun sequence.
ACCESSION   DASSFP010000205 DASSFP010000000
VERSION     DASSFP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49493"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1015)
                     /locus_tag="VFH91_04405"
     CDS             complement(218..1015)
                     /locus_tag="VFH91_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6862259.1"
                     /translation="MLCEETRQSLSFYVDNVLTLPARVAIDEHLDRCPVCRSELSDLR
                     SGRRSLSLLSRPVPPPDLTVNIVDALIIESAARRQAPPPSLSYRVAHFLQPRLMPYTI
                     GSFVSVIMFFLMFSALRPHFVALREAALQRNGVILLRSPEIVYDLNQPVTPEVFAASR
                     APFAEQSPSLNPGGALAALTRAYAHPHRNYYEDSDDMIVVADVFSNGAASLADVVQPP
                     RNKRMLSDFESALRQDAAFVPASLDGRPDTMRVVFAVQKVDVDDQNF"
     gene            complement(1349..2005)
                     /locus_tag="VFH91_04410"
     CDS             complement(1349..2005)
                     /locus_tag="VFH91_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6862260.1"
                     /translation="MSYGEPIVLRGQFELEQVAPATSVDVRPKIEDQFLERLKRGDVA
                     AFETLVSERSGEIYGLLYRLTENVDEARDLTQETFLRAFQSISHFRGDSDIRTWIYRI
                     AINQARNRWRWWRRRKRDLTVSLDSNETKSHELLVANLKATTVDPERDALSRERERLL
                     RKALSTLKQVYREAVILRDIEGFAYEEIAIALDISVGTVKSRLARGRQELKRKLEGSL
                     "
     gene            2292..3095
                     /locus_tag="VFH91_04415"
     CDS             2292..3095
                     /locus_tag="VFH91_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862261.1"
                     /translation="MANYNKKRARELQHDKFRDTTMTLVDRVLNRLEGQGRMILYVLG
                     GIIVVGVLVLLLMRWNARKNDEAQQALGRAIDISTATVSAAAPQTGAEPAFSNDRDRA
                     QQAIQEFEKVAAKYGDPYKSEARYFSATNQLIVDRQKGMAELAQLGNSSVTEVAALSK
                     FALAQAKESDGKYDEAAQLYQDLVKLNNPTVTAETANLRLAKVYQKQGKKKEATDLLF
                     NIVDASRKAKGPDQSPIPASSAAREAASELQRMDPERYAQLPPELPTLG"
     gene            3133..4281
                     /locus_tag="VFH91_04420"
     CDS             3133..4281
                     /locus_tag="VFH91_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET6862262.1"
                     /translation="MDNLTHSLVGLAVAKAGLEKLSPGATTVCVLAANAPDIDVAVLL
                     FADRWTFLREHRGITHAIVGTICLAVLLPLLLYGIDYAISRFRNTTPTTKLKGLFIAS
                     LIASVTHPIMDWTNNYGMRFLLPWNERWFYGDFVFIVDPFLWLVLGTSVFLVTSRTRF
                     QRITWITIAGILTALLLLAPRSGDLQHPVFVRAIWFVFLISGIVLFVFKVGERWKERV
                     VFGGIAIALCYWIGLFLIHQHAVNRSRARADEIARSNSETVINLAAMPVLASPFNWDC
                     VFDTDKATYRFQVSLVTDDRAPGVIRYAKPTGELATITEKLAKNERSARIFLGFARFP
                     VVRLEDPDCTTQTIVQLADLRYTEPGRSRGTFALDLPVDCQTELVGQK"
     gene            4278..5684
                     /locus_tag="VFH91_04425"
     CDS             4278..5684
                     /locus_tag="VFH91_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862263.1"
                     /translation="MKETDQLESLTALADAKKDRKSNPLASVRVSPGPYFAVASVLTF
                     LSVLLLRSNHDSWALLLIGVAWLVVPALAFMDRIVFDGGSIRRQGPISAVMGLIYGYR
                     KHLNVSDFELLETHAVRTLRRGGSVRYRYRTQIMGKGKEFVIASGGHGYRKLVRELFP
                     LIHEDKLDNRTRDLRDYLNDPNVLSNKAKLSSLASEDVLDLTTADRSLRMKQQRQPVV
                     VEPHDIERAHLLRRLGNELRVSGRLKEAAEAFRRSLKVTPRASWLLYDLARLLRSQAS
                     AQGDARLLSRARAALRLARIRAKDDVVLLPLIGESFLECGDTRQARRVLQDTVDLDYG
                     NYKARLGLADLSLREGKLAHVIHHYHEAARVASETALARYARREADYYTRLNDDEDYL
                     VTELRRISWLQNVTRVRKLAGRVTSVAILVALTGGFLDPLAGSIGWSLASSALILWLL
                     SVVVTRVLFSRSKPDPVA"
     gene            complement(5744..>6426)
                     /locus_tag="VFH91_04430"
     CDS             complement(5744..>6426)
                     /locus_tag="VFH91_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862264.1"
                     /translation="AFLKPFDGGGWKNVSKVNSLDELWAEYDKTGTLCMTLQESIEFD
                     QFVRCYCVGQEEVMIMAYDPRKPYLSGEQYVHDPNYLAPQLAERVKKDVRTLCTALGY
                     DINTVEFAIKDGVPYAIDFMNPAPDAELTSVGEFYHNWIVEAVAKLALKRLSESEGPV
                     RYRWDSLLNPEPVRAYAVASASDQQSRTVVPATPGTTTKATTQTAVVSKKPVQAKKTT
                     VRRKKPTA"
BASE COUNT         1580 a         1627 c         1628 g         1591 t
ORIGIN      
        1 ggtgcgtcaa gcgagacagt gagaagcgtg agttgaattc tctggcgatc ctaaaacaga
       61 gtcagaatcc aggcggcgca actggaaagt aatcgacaac ggatttatcg ttccgaactc
      121 atttcggtat tggtctatgt atttaaagat agattcgggg acagttggga attatccaaa
      181 gctgggtgag atggcttgag atgtagggac gagtacctta aaaattctga tcgtcgacgt
      241 ccactttttg aaccgcaaaa actacgcgca tcgtgtctgg tcgaccgtct aaagaagctg
      301 gaacaaaagc cgcatcctga cgcagagctg actcgaaatc acttaacatt cttttgttcc
      361 gaggcggttg taccacgtca gccaatgacg ccgcaccgtt actgaagaca tcggcaacaa
      421 caatcatatc gtcagagtct tcgtaatagt tccggtgcgg atgagcataa gcccttgtca
      481 atgctgcaag tgctccaccg ggatttaagc taggcgactg ttcagcaaaa ggggccctgc
      541 tcgccgcaaa gacttccgga gtgactggtt gattaaggtc gtaaacgatc tcagggctac
      601 gaagcagaat cactccgttt cgctgaagtg cagcctctcg aagcgcgaca aaatgaggtc
      661 tcagcgccga gaacatcaga aagaacatga taaccgacac gaaggaaccg atcgtgtaag
      721 gcattagccg cggctgaagg aaatgagcga cccgataact caatgatggc ggtggtgcct
      781 gacgtctcgc ggcggactca ataattaaag cgtcaacaat atttactgtt aggtctgggg
      841 gcgggactgg tcttgagagg aggctgaggc tacgacgtcc tgaacgcagg tcagacagct
      901 ctgagcggca aaccgggcag cgatccagat gttcgtctat cgcaacgcgc gccggcagtg
      961 ttaacacatt atcgacgtag aaggaaagcg attgtctggt ttcttcgcat aacataggta
     1021 atgcacccac ttgtcgacct tcagttgctc cattgcgcaa ctaaaaatcg tttgactttg
     1081 gatagctcac caaccccggc tgctcccttt ttgttaagaa gcaactccga cagatttggg
     1141 caaccccttc aggccgaatg cgagcgatgc ttcaaaacgg gtgttgctgg ccacgttttc
     1201 aaattcactc tcatcacgcc atttcggaag ccttccgccc gccgtttcct cacttcgacc
     1261 catttggcac cgagtttcgt gagccggggt tggaaagcta tccaaactgt tgccgattga
     1321 ctcttcatcg tcgaagagaa tcggtttatt acaaagatcc ttctaacttt cgcttcaatt
     1381 cctggcgtcc acgcgccagc cgggacttca ctgtccccac gctaatatcc aatgctattg
     1441 cgatctcttc ataagcaaag ccttcgatat cacgaaggat cacggcttct ctatagactt
     1501 gtttcaatgt actcagagcc ttacgaagca gtcgctcgcg ctcacgagac agagcgtctc
     1561 tctcaggatc gactgtcgtg gctttcaagt tcgcaaccag cagttcgtga ctcttggtct
     1621 catttgaatc cagcgagact gtgaggtcac gttttcgccg ccgccaccag cgccaccgat
     1681 tccgggcttg attaatcgca atgcgataaa tccaggttcg aatatcggag tcgcctcgga
     1741 agtggctgat actctggaac gctctcagaa aagtctcttg agttaggtcg cgcgcctcat
     1801 cgacgttctc ggtcagccgg tatagcaaac cgtagatttc acccgaccgt tcggatacaa
     1861 gcgtttcaaa ggctgcgaca tcaccgcgtt taagtctttc aaggaactga tcctcgattt
     1921 ttggccgtac atcaaccgag gtcgccggcg caacctgctc taattcaaat tgcccacgga
     1981 ggactattgg ctctccgtag ctcacgtttc accccctggg gcgggaatct gagcagctat
     2041 caatcgccca tttattgcca ggcgtctcat tagacaccag taaaagtcag gttgttccag
     2101 aaaaattttc atccagtgtc ttggatgcat ccagttccta tcgaagcagc cactgacgca
     2161 tcgcaaccct ccaacagcag gtcatttgag ctgcttgaca agttttcgta cgcgccatta
     2221 gagtgacatt ttcgtttatc atacacccaa aagaacgtag ttcactatgt ggacttgcga
     2281 gaggaaaaga agtggctaac tacaacaaga agcgagcccg ggaacttcag cacgacaaat
     2341 tccgagacac gacgatgacc ctggtcgacc gggtccttaa tcgactcgag ggccagggcc
     2401 gaatgattct atatgttttg ggaggaatca ttgtcgtcgg cgtgctggtt ttgcttctca
     2461 tgagatggaa tgctcgcaag aacgacgagg ctcagcaagc tttgggcaga gcaatcgata
     2521 tttcgaccgc aaccgtttca gcggccgcgc ctcaaaccgg cgcagagccg gctttcagta
     2581 atgaccgaga tcgggcgcaa caggccatcc aggagtttga gaaggttgcc gctaaatatg
     2641 gcgaccctta caagtctgaa gcgcgttact ttagcgccac taaccaattg atcgtcgaca
     2701 ggcaaaaagg tatggccgag cttgctcagc tgggcaacag cagcgtaact gaggtagctg
     2761 cgctctcgaa gtttgcgctt gctcaggcaa aggaaagcga cgggaaatat gacgaggcag
     2821 cccaactcta tcaagatctc gttaagctca ataatccgac cgttaccgcc gaaacagcaa
     2881 acctaagatt ggcaaaggtc tatcaaaagc agggtaaaaa gaaagaagca acagatttgc
     2941 ttttcaatat tgtcgatgct tcgcgcaaag caaaaggacc agaccaatcc ccaattcctg
     3001 cttcgtcagc agctcgtgaa gctgccagtg agctacagag aatggatccc gaacggtacg
     3061 ctcaacttcc ccccgaactc ccaacactcg gctagaaaca gaccgaaact aatccttaag
     3121 ctagtctcgt aaatggataa ccttacccat tcactggtcg gactagcggt tgccaaggca
     3181 ggcctggaaa aattatcgcc cggtgcgacc acggtctgtg tactcgctgc caatgctccc
     3241 gatatcgatg tggcggtcct attgtttgcg gatcgctgga cttttctgag agagcaccgc
     3301 ggtattactc acgcgatagt cgggacaatc tgtctagctg tgctgttacc actattactc
     3361 tacggcattg actacgctat ctctcgcttt cgcaacacga caccaactac caaattgaaa
     3421 ggtttgttta ttgcgtcact gatcgctagt gtcactcatc cgataatgga ttggaccaac
     3481 aactacggca tgcgatttct gcttccctgg aacgagcgat ggttctatgg ggattttgta
     3541 ttcatcgtcg atccatttct gtggttggtg ctaggtacca gtgttttcct ggttacttcc
     3601 aggacgcgtt tccagagaat tacgtggata acaattgccg gaatcttgac agcactgttg
     3661 ctacttgctc ctcgatcagg tgaccttcaa catccagtat ttgtaagagc aatttggttt
     3721 gtattcttaa tttctggaat tgtgctattt gttttcaaag tgggtgaacg ttggaaggag
     3781 agggtggtat ttggtggaat cgcaatagca ctctgctact ggattggact cttcttgatc
     3841 caccagcacg ctgtcaatcg gagccgtgct cgcgcggatg aaatcgccag atcgaactcc
     3901 gaaactgtca taaatctcgc cgctatgccg gttctagcga gcccttttaa ctgggactgt
     3961 gtcttcgata ccgataaagc aacctacaga tttcaggtta gtttagtaac tgatgatcgg
     4021 gcccctggtg tgatcagata tgcgaagcca actggtgagc tggcgacgat taccgagaag
     4081 cttgcaaaaa atgagcgtag cgcacgcatc ttcctcggtt tcgcgagatt tcccgtggta
     4141 cggcttgagg accccgattg cacaactcag actatcgttc agctggctga cttacgttac
     4201 actgaacctg gcagaagcag aggaaccttc gcactggatc tgccggttga ttgtcagacg
     4261 gaattggttg gacagaaatg aaggagaccg atcaactcga gagcctaacg gccttggcag
     4321 acgccaaaaa ggatcgcaaa agcaatcctt tggcaagcgt gcgggtgtct cctggaccat
     4381 atttcgctgt cgcaagcgtt ctgacttttc tgtcagttct gttgctccgg tcaaatcatg
     4441 acagttgggc actgctctta atcggcgtcg cctggttggt tgttcccgct ctcgctttca
     4501 tggatcgaat tgtctttgat ggtggttcca tccgccgaca aggaccgata tctgctgtaa
     4561 tgggattgat ctacggctac cggaaacacc tcaatgtctc agacttcgag cttcttgaaa
     4621 cacatgcagt gagaaccctg cgtcgtggcg gtagcgtacg ctaccgttat cgcactcaga
     4681 taatgggaaa aggcaaagag ttcgttattg cgtcaggcgg gcacggatat cgaaaactgg
     4741 tacgcgaact ttttccgctg attcacgagg acaagttaga caaccgaacg cgcgatctta
     4801 gggattacct gaacgatccg aatgtgttgt cgaataaggc taagctgtcg agcctcgcat
     4861 ccgaagatgt tctcgattta actacagccg acagaagcct acgaatgaaa caacaacggc
     4921 agccagttgt ggtggaacct catgatattg agcgagcgca tctgcttaga agattaggaa
     4981 atgagctcag agtctcgggt cgtctgaaag aagcggccga agcatttcgt cgttcgctta
     5041 aggtcacgcc tcgagcgtca tggttacttt atgacttagc ccgtttgttg cgatctcagg
     5101 caagcgcaca aggcgatgcc cggcttcttt cgcgtgcaag ggcagcattg cgactcgcaa
     5161 gaattagagc gaaagacgac gtcgtgctct tacctcttat aggcgagagc tttctggaat
     5221 gcggtgatac tcggcaggct cgtcgcgtac tgcaggacac agttgatctc gattacggaa
     5281 attataaagc tcggctcgga ctggctgact tatcactcag ggaaggaaag ttggcccacg
     5341 taattcatca ctatcacgag gcggctcgcg tagcctctga gaccgcgttg gccagatacg
     5401 cacgccggga agccgactac tatacgcggt taaatgacga cgaggattac cttgtcactg
     5461 agttacgtag aatcagttgg ttacaaaatg tcacccgtgt tagaaagctc gccggtcgcg
     5521 tcaccagcgt agcaatattg gtggcgctga ctggaggttt tctggatcct cttgcgggaa
     5581 gtatcggttg gtcactcgcg tcgtcggcat tgatcttatg gttgctgtcg gtagtggtaa
     5641 ctcgagtcct tttctcgcga tccaaaccag atcccgtcgc ctgaaatgaa aaagggcagt
     5701 gcgtttaaag caccaccctt tttagaaatt agatgtttct tagttaagca gttggctttt
     5761 tgcgacgtac ggttgtcttt tttgcctgta caggcttttt gctaaccacg gctgtttgag
     5821 ttgttgcctt ggttgtagtc cctggcgttg ccggcacaac tgtcctcgat tgctgatcag
     5881 acgctgacgc gactgcgtag gcgcgaactg gctctggatt caacagtgag tcccaacggt
     5941 atctaacggg tccctctgat tcactgagtc gtttgagcgc aagctttgca actgcttcga
     6001 caatccagtt gtgataaaac tcacccaccg aagtcagttc ggcatccggg gccggattca
     6061 taaagtcaat cgcgtaaggc acgccgtcct tgattgcaaa ctcaactgta ttgatgtcgt
     6121 aacccagggc ggtacacaaa gtgcgaacat ccttcttcac tctctctgct aattgcggcg
     6181 cgaggtaatt gggatcgtga acgtactgtt caccggataa gtatggctta cgaggatcgt
     6241 aggccatgat cattacttcc tcctggccga cacagtaaca gcgaacgaat tgatcaaact
     6301 ctatcgactc ctgcagcgtc atgcagagcg tcccggtctt gtcatactcg gcccataact
     6361 catcaagtga atttaccttc gagacgtttt tccaaccgcc gccatcaaaa ggtttgagaa
     6421 acgcgg
//
LOCUS       DASSFP010000206         5746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1825968, whole genome shotgun sequence.
ACCESSION   DASSFP010000206 DASSFP010000000
VERSION     DASSFP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825968"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(577..1116)
                     /locus_tag="VFH91_04435"
     CDS             complement(577..1116)
                     /locus_tag="VFH91_04435"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6862265.1"
                     /translation="MAGTNTTQTGTALSRDEKTNPRTQKITTFLWFDNNAEEAANYYV
                     SIFKNSKILNTSHYGDAGPGPKGTVMTIGFVLDGQEFTALNGGPLFKFNESVSQVIHC
                     KTQDEVDYFWEKLSEGGQKVECGWLKDKYGLSWQVVPDVLFELITDKDREKANRVTKA
                     MLQMKKLDIAGLQKAAAGN"
     gene            1316..2389
                     /locus_tag="VFH91_04440"
     CDS             1316..2389
                     /locus_tag="VFH91_04440"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET6862266.1"
                     /translation="MQLPINPPVLPMLAKRVSELPQTGSWFFEPKWDGFRALVFRDGD
                     EVLIQSRDTKPLNRYFPELIEPLKKQLPTRCVLDGEVVLAKNGALDFDALQLRIHPAA
                     SRVKMLSEQIPASIVFFDLLAEKNRDLTATPFEARRALMEKLLSSAAPPIHITPITAS
                     LDVATDWFRRFEGAGLDGVVAKPTSGNYEANKRVMLKVKHERDCDCVVAGFRWHKKGE
                     RSAIGSLLLGLFDKSGELHHVGVCASFTDKKRSELVEFLEPYRKNALKDHPWKNWASE
                     ELASDRRIPGAQSRWSQGKDMAWEPVRPELVVEVAYDHMQGSRFRHTAQFRRWRVDKS
                     PAECTYAQLEVVPPQEISEIFGR"
     gene            complement(2604..3011)
                     /locus_tag="VFH91_04445"
     CDS             complement(2604..3011)
                     /locus_tag="VFH91_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862267.1"
                     /translation="MPEVNRCLMVVKPKQPFLDWLNSLDTEDDMNLTLEELQNDSTAY
                     LVPEYGTPEDQAEIISWCHNYVFEEELMAWYTFEEEWPQARDLNAFLEWFEVEFHSVV
                     MDLDLEVPLQHVEYDVDVEDNDESEVDPSSNGH"
     gene            3095..4420
                     /locus_tag="VFH91_04450"
     CDS             3095..4420
                     /locus_tag="VFH91_04450"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HET6862268.1"
                     /translation="MKNEAEAEVISIDGREVRVTHPDKPYFSVATKLSKLDLVRYYLS
                     VAPGALNGIRDRPIVLKRFVDGAEGQAFYQKRAPANIPPWLRTVTLSFPSGRKAEEMV
                     VDDPAGLAWIVNLGCIELHPHPVLSGDLVHPNELRVDLDPGPGVGWEDVRTVALEVRS
                     LLEELGLRGWPKTSGSRGMHVNVRIQQRWTFVEVRRAALALSRAIERRLPDRATSKWW
                     KEERHGVFLDYNQNAKDRTTCSAYSVRPLPDARVSTPLDWSEVADCNPADFTVLTVPQ
                     RFAEIGDPHTEMDSHAGSLEPLLELAERDEAAGLGDAPWPPHFRKMEGEAARVAPSRA
                     KSTAKPARVKMPLIVIANSPDKNAALAGLEKWKRKHKKVAKLIGVEDILVDTMRGRSS
                     TWTRIRVNLRNVPEELRPSQEKPDPDDDPTREWREWRAANKKPSKKKDA"
     gene            4771..>5746
                     /locus_tag="VFH91_04455"
     CDS             4771..>5746
                     /locus_tag="VFH91_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6862269.1"
                     /translation="MKFAKLVLKNILRNKRRTLLTISSLVVSLFLIITLATILTEFER
                     GSDESSPLRLISRHAVSLAFNMPMAHLQRIKTVPGVKEAMPFNWFGGIYKDEKNFFAN
                     FAVDASKLRDIMAELKMSDAEWQAFINDRQGAIVGQKLVKLYGFTPGQRVTLKSPIYN
                     KEVEFIIRGVCTGGDEKTLFFHQDYLNELLPEWAKDKASTFSIRANTADDVPRIAQAV
                     DSIFANTDAPTKTESESEFAKSFETMMGGVKQFLYGIMAAITFSVLLVMGNTMAMTVR
                     ERTKEVGTLKAIGFQRGTITALFLAESLIVACIGAAIGIGGASLIFRSI"
BASE COUNT         1456 a         1427 c         1437 g         1426 t
ORIGIN      
        1 tttatctttt gccactatta cttgtcccac caatcagatt ttagcgtgat gcctagaaag
       61 gcgaggcatc ataaatctat gatgcctaga ttgtcaaagt attgagattg agctagtgtc
      121 ctgtctctaa agttcgtaga aagaaacgaa acagtttggc gaacgaggtt gttggtaaag
      181 ggcagggccg cgacgcaaac ttaactaata ataaccgcca ggttggatgt gttgattggc
      241 aggctttcac tccggaggag tgacatgctt atagacttgg tcctacttct ctctcctcca
      301 ctccgttcga agttctcaga aggagcgact aggaatgtac gcttcaagaa ctctaccgct
      361 cctcttagaa cttcgaacgg agtgggacgt cctggggagt tgtcggctat aaacatgtca
      421 ccccgcgggg gtgaaaccct atgctattcc atttgctaac aaatcggctc ttgcattttc
      481 cattttgacc aagataacca ctcttaacac aacgagcgct catgaaatga aaaatgacca
      541 atgacaaata atccggcgcc tgccatcatt gcccgctcag ttaccggcgg ctgctttctg
      601 cagacctgcg atatcaagct ttttcatctg cagcatcgct ttggtaaccc tgtttgcttt
      661 ctcacggtcc ttatcagtga ttaattcgaa caggacatcc ggaaccactt gccacgatag
      721 accgtacttg tcttttaacc agccgcactc aactttttgg ccaccctcgg aaagcttttc
      781 ccaaaaataa tcaacctcat cctgcgtctt gcagtgaatc acctgcgaga cggattcatt
      841 gaatttgaat aacggccctc cgttcaaagc ggtgaactcc tgcccgtcaa gcacaaaccc
      901 gatcgtcatt accgtgcctt tgggacctgg accagcgtcg ccgtagtggg aagtgttcaa
      961 aattttcgaa ttcttgaaaa tcgaaacata gtagttcgct gcttcttcag cattattgtc
     1021 gaaccaaaga aaagtcgtaa tcttttgggt tcgcggattc gtcttttcat ctcgggaaag
     1081 tgctgtacct gtttgagtcg tattcgttcc tgccatacct tctccttctg ttgctttcta
     1141 attaatttct tgttgtcgaa ctccagacgt cgtctcggtt cggggagcag tcaacagtta
     1201 atcgcctacg gagggccaga atcgacaatg ttgccaaaag ttttttcgga aagtttgtca
     1261 caacgaagtc gattgccgct tcgcccaaca gggattcgag ttatatgatc cggcgatgca
     1321 actgccaatc aatccaccag tcctaccaat gctcgccaaa cgtgttagcg aattgccaca
     1381 aacaggatcc tggttctttg aaccaaagtg ggatggcttt agagctctcg tgtttcggga
     1441 cggcgacgaa gtcctgattc agagtcgcga cacaaagccc ttgaatcgat atttccccga
     1501 actgattgaa ccgttaaaaa agcaactgcc tacacgatgt gtacttgatg gcgaagtagt
     1561 actcgcaaag aatggcgctc tggatttcga cgctttgcaa cttcgtatcc atccggcggc
     1621 ctcgcgcgtg aagatgcttt ctgaacagat cccggcttcg atcgtgttct tcgatctact
     1681 tgcagaaaaa aaccgtgacc taacagctac cccctttgag gcacgacgag cactgatgga
     1741 aaagcttttg tcatcggcgg cgccacccat tcacataacg ccaattaccg ctagcctgga
     1801 cgttgcaact gactggtttc ggcgatttga gggagcagga ctcgacggtg tagtcgccaa
     1861 gcccacgagt ggtaactacg aagccaataa gagagtgatg ctcaaggtaa agcatgaacg
     1921 agattgtgat tgtgtcgtcg cgggttttcg ctggcataag aagggagagc gaagcgcaat
     1981 tggttccttg ctattagggc tgtttgataa atcaggagaa ctgcaccatg tcggtgtgtg
     2041 tgcaagtttt acggataaga aaagaagtga gcttgttgaa ttcttagaac cctacaggaa
     2101 aaatgcgctc aaagatcatc cgtggaagaa ttgggcgagc gaggaactcg cgagtgatcg
     2161 tcgaattccg ggagcccaaa gtcgttggag ccagggaaag gacatggctt gggaaccagt
     2221 gagacccgaa ttagtagtgg aagttgcgta tgatcacatg cagggatcga ggttccgcca
     2281 caccgcgcaa tttcgcagat ggcgagtgga taagagtccc gcggagtgta cgtatgcgca
     2341 actggaagta gtaccgccac aggaaatttc ggagatattt gggaggtagc cagggggttt
     2401 cggaagagga gaattgggca gttatttcat ttttcatttc tcatttctca tttttcatgc
     2461 gctcatttgg tccgtcgcga aatgacttct tgccaatgct cgaattgttc acggctcgtc
     2521 ttcagaaacc tgacgactcc cgcctcgaaa aatgaaaaat agaaaatggt caagacctag
     2581 accattccgt caacccaatc cctttagtgc ccattcgaac tcgggtctac ttcagattcg
     2641 tcgttatcct caacatcaac atcgtattcc acatgctgca gaggaacctc caaatcaaga
     2701 tccatgacaa cactgtgaaa ttcaacctca aaccactcca aaaacgcatt cagatctctc
     2761 gcctgaggcc attcctcctc aaacgtgtac caggccatta gctcctcttc gaagacataa
     2821 ttatggcacc aggaaataat ctcagcttga tcctcaggag ttccgtactc tggcaccagg
     2881 tatgcagtcg agtcgttctg taattcctcg agcgttaaat tcatatcgtc ttcagtatca
     2941 agcgaattca accagtcaag aaaaggctgc ttgggcttaa ccaccatcag gcatctgttt
     3001 acttcaggca tgtttcagag aataccttac ttggacattg agggaaacac attgggactg
     3061 tttggctaca ccatgaacat gctagcctgt gcgcgtgaag aacgaagcag aagccgaagt
     3121 catttcgatc gatggccgcg aagtacgggt tacgcatcct gacaaacctt atttctctgt
     3181 tgccaccaag ctctcgaaac ttgatctcgt tcggtattac ctgtcggtag cacctggagc
     3241 cctcaacgga atacgcgatc gcccaatcgt tctcaagcgc ttcgtcgatg gagctgaggg
     3301 ccaggctttc taccagaaac gcgcaccggc gaacatacct ccctggctca gaacggtcac
     3361 actgtctttt ccgtccgggc gaaaagcaga ggaaatggtg gttgacgatc ctgccggcct
     3421 ggcttggatc gtgaatctgg gctgtattga gttacatcca catccggtcc tgtcaggaga
     3481 tctggttcac ccaaatgaat tacgagtcga tctcgatcct ggcccaggcg tcgggtggga
     3541 agacgtgcga acagtggcac tggaggtgag gtcgcttctt gaagaactcg gccttcgcgg
     3601 atggcccaag acgagtggct ctcgaggaat gcacgtcaac gttcgtattc agcaacgctg
     3661 gacctttgtc gaggtacgtc gtgcagcgtt ggccctatcg cgcgcaattg aacgtcgtct
     3721 gccagaccgt gccacttcaa agtggtggaa agaggaacga cacggtgttt ttctcgatta
     3781 caaccagaat gccaaggacc ggacaacctg ttcggcctac tccgttcgcc ctcttcccga
     3841 cgctcgtgtc tcaactcctc tcgattggag tgaggttgct gactgcaacc ctgcagattt
     3901 cactgtactg acggtgcctc agcgcttcgc ggaaattggg gatccacaca cggagatgga
     3961 tagtcatgcg ggatctctgg agccgctgtt ggaattggca gagcgtgatg aggcagcggg
     4021 tctgggcgac gcaccgtggc ctcctcactt tcgcaagatg gaaggcgaag cggctcgggt
     4081 ggcgccatca cgtgcaaagt cgactgccaa gcctgctcgt gtgaaaatgc cactaatcgt
     4141 gatagctaac tctcctgaca agaatgctgc tcttgcaggt ctggaaaagt ggaagcggaa
     4201 gcacaagaaa gtcgcgaagc tgatcggcgt tgaggatatt ctggtcgaca ctatgcgtgg
     4261 ccgatcatcc acgtggacac gaattcgagt gaaccttcga aatgttccag aggaactgcg
     4321 accgtcccag gagaaacccg atccggatga tgatcccact cgggagtggc gagaatggcg
     4381 agcggcgaat aagaaaccgt ctaagaaaaa ggacgcctag tttgggtcgc caaagcgaaa
     4441 acctaaaaca atctaaataa ctaatcgaac gaaacaatag attttatgac tcaggctgtc
     4501 tatttgagct gtgctcattc gacttacata cagaagagcc accgaatggg cgcttctgag
     4561 ctgaactggg ccttgagtga gttagagact ttgctcaaag tattgtaaat tgagctaccg
     4621 aatttattcg tttccaaggc cgtaaccaat atccccgccg ggagttatac aatgcggtgg
     4681 atttctgtga accctttccg ccgaggttgc gtactcgctc tagagtactt tctgctctgg
     4741 cgatagcttt taaaaatcag gtgacatctt atgaagtttg cgaagcttgt cctgaagaac
     4801 atcctgcgga acaaacgtcg aaccttactc acaatttcga gtttggttgt ttcgctgttt
     4861 ttgatcatca ccctggccac gatcctgacg gagtttgagc gaggcagcga cgaatccagt
     4921 ccattgcgac tgatatcacg tcatgccgta tcgctggctt ttaatatgcc gatggcccat
     4981 ctccagcgaa ttaaaaccgt acctggggtt aaggaggcaa tgccgttcaa ttggttcggc
     5041 ggaatctaca aagacgagaa gaacttcttc gcgaacttcg cggtcgacgc gagtaagctg
     5101 agagatataa tggccgaact taaaatgtcc gatgcagaat ggcaagcctt catcaatgac
     5161 agacaaggtg cgattgtggg ccaaaagcta gttaagctct atggctttac acccggtcag
     5221 cgcgttactt tgaaaagccc aatctacaat aaagaggtcg agttcataat tcgcggcgtt
     5281 tgcactggcg gtgacgagaa aactctcttc ttccaccagg actacctcaa cgagctgctg
     5341 cctgaatggg ccaaggacaa agccagtact tttagtattc gcgcaaatac tgccgacgac
     5401 gtacctcgta ttgctcaggc ggtagattcg atttttgcca acaccgacgc cccaacaaaa
     5461 acggagagcg agagcgaatt cgccaagagc tttgaaacaa tgatgggcgg cgtcaaacag
     5521 tttctctacg gcattatggc tgcaatcaca ttttcggtgc tgcttgtcat gggcaacacg
     5581 atggctatga cagttcgcga gcggaccaag gaagtcggca ctctgaaagc gattgggttt
     5641 caacgaggaa cgatcacagc gctttttctc gccgagtcgc tgattgttgc gtgtattgga
     5701 gccgcgatcg gtatcggagg cgcatcgttg atctttcgct ctatcg
//
LOCUS       DASSFP010000207         8016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_50770, whole genome shotgun sequence.
ACCESSION   DASSFP010000207 DASSFP010000000
VERSION     DASSFP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50770"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFH91_04460"
     CDS             <1..207
                     /locus_tag="VFH91_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET6862270.1"
                     /translation="DVRIGADSLVAAGSLVSPGTIIPPRSLVMGFPARVKRELTDDEV
                     RGLTVFWKNYVDYTREYLEEERRG"
     gene            409..1800
                     /gene="hisS"
                     /locus_tag="VFH91_04465"
     CDS             409..1800
                     /gene="hisS"
                     /locus_tag="VFH91_04465"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET6862271.1"
                     /translation="MPSAKTQPARGMRDFLPDEVRKREFVISVIKEVYEKYGFEPLET
                     PAVENIETLTGKYGEEGNQLIFKILKRGVHESTGEADLALRYDLTVPLARVVAEYRDK
                     LPKFFKRYQIQPVWRADRPARGRFREFFQCDVDVLGSRSVTVESEICAAANDVLVRLG
                     FNDFCIRVNHRKILTGILGVSGVPLEKHDLALVALDKLDKVGREGVQKEFESRAIDPV
                     SGERLLDFFAELNELDQAAEIAAGEDPERKQQALNQAVIGRLVEFVGNNEVGALGVSE
                     LQSILDYGEASAIGPRLKIDPSLARGLSYYTGAIMEINVKDLPGSLGGGGRYDNLVGM
                     FLGQDVPACGFSLGLERILVVMSERGMFPPEVGTTPADIMVTIWNEESVDDSIRLARE
                     LRTIGLRVDLYPEADKLGKQFKYASSRGVPLVALLGDDERAKGEVAIKDMRTGEQRTY
                     KRAELDTAMIRAV"
     gene            1823..2422
                     /locus_tag="VFH91_04470"
     CDS             1823..2422
                     /locus_tag="VFH91_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HET6862272.1"
                     /translation="MASDDVVKDYLSDSIAMFRSYKKMVDNAIIQVNDDELFATIDEE
                     SNSIAIVMKHLAGNMLSRWTDFLTTDGEKPTRNRDMEFVLESKNKGELLDYWERGWRC
                     VFDALEPLQLEDFEKTVTIRGEEHTIVKAINRQLSHYAYHIGQIVFLAKHFRSSEWKS
                     LSIPKNKSAAFNQYLQQGKGAGQDRMDAAMTFAKDTDSQ"
     gene            2459..2827
                     /locus_tag="VFH91_04475"
     CDS             2459..2827
                     /locus_tag="VFH91_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6862273.1"
                     /translation="MDTKLHHVNVTVPKSLETDAKHFYGVVMGLTEVPKPAESRGRGG
                     AWYQLGPMQLHLSIEDGLGAECISKRHVCYTVPDLAVAEHKFREAGVEILPDDIPTPG
                     WTRFYVRDPGGNRLEIAQDN"
     gene            2861..4669
                     /gene="aspS"
                     /locus_tag="VFH91_04480"
     CDS             2861..4669
                     /gene="aspS"
                     /locus_tag="VFH91_04480"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET6862274.1"
                     /translation="MLDNLGDLERTHSCGQLRASDIGKQVLLMGWVAKRRDFGDLTFV
                     DLRDRDGITQVVFNAESAAEAHTKAKDLRSEYVIALKGEVVPREENTRNPKLLTGDVE
                     VHARELFVLNDARTPPFQLDASPEGLASEDLRLKFRYLDLRRPQLQSNLRLRHRVVTE
                     IRRYMSAQGFTEIETPILIKSTPEGARDYIVPSRIQPGKFFALPQSPQLFKQLCMIGG
                     LDKYFQIAHCFRDEDLRADRQPEFTQLDVELSFATLTQVYALIEGLFASVFKLIDVEL
                     PTPFPRLSFEEAIRRFGSDKPDLRIKGMELQDISGVLTSTEFPPFASSLAAGGQIKAI
                     VVPGGANLSRKVLDDLQEFVRRYGASALAWIKLGDETSSSLLKALGPDLVARIAQTTG
                     AASNDAVLIVSGKAKVVAASLGALRNEVAKREQLIPQNVYAPLWVTEFPMFEYHEEDG
                     RYYSMHHPFTSPVDEDLNLLERAVEGGETELLAKIRTKAYDPVINGVEMASGSIRIHR
                     RDVQRLVFKALGMTTEEARERFGFFLDALEYGTPPHGGIAPGIDRLMMVLAGTDNMRD
                     VIAFPKTASAADLMIDAPGEVDSRQLDELHLKVVQD"
     gene            4724..5014
                     /locus_tag="VFH91_04485"
     CDS             4724..5014
                     /locus_tag="VFH91_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862275.1"
                     /translation="MTHKGSVRVLLFIGSNLLLLSYKACEEGDAHLSYSCVPVSDASP
                     PFLGFGEWISVGIFLFVVASGVLVSAVMILTQHYRCSVYESIGLQLETHRKM"
     gene            5060..5482
                     /locus_tag="VFH91_04490"
     CDS             5060..5482
                     /locus_tag="VFH91_04490"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET6862276.1"
                     /translation="MVALQLRREHFLALVDHARETAPAECCGLLGGTETAKAESIYKL
                     RNVTTNPETGYEAAPEDLFAAQRQMRDRGEELLAIYHSHPRASNPSPSETDVRLAYYP
                     DATYLIVGLGGAEPVVKAFRISERERSWHPVEYVITGE"
     gene            5498..6184
                     /locus_tag="VFH91_04495"
     CDS             5498..6184
                     /locus_tag="VFH91_04495"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET6862277.1"
                     /translation="MEGNKSKYDTNPLDPDVERTTSEAWGDGASAGQTQPVQGITQKV
                     GGSQEDSSRKNVYSEAPTRRYDSPPLETPYPSVFVPPTYSPPVKQQFTGSPYQPPRQS
                     SSPAHSVSGFGIPERWATMLAYTPSYIGLVVSLLELFLVPRNEVKVRFHASQAIAIHI
                     GILIVQTVFAAIHAITGSSIGGTLFGVLSFVFLIISMIKVWRGDSHRIEPVAEAAQWF
                     NQHIEPRKQG"
     gene            6406..7806
                     /locus_tag="VFH91_04500"
     CDS             6406..7806
                     /locus_tag="VFH91_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6862278.1"
                     /translation="MIKQSRFIAWLAVVAIFSMPLSAVAQTPIKYHSNKYSVQDDIKL
                     GRQAAQEAEQQFPLLNDETVRSYVARVGDRLVRAIPNQFQHPEFRYYFKVVNASDINA
                     FALPGGPMYVNRGMIQAARNEGEMAGVMAHEISHVALRHGTAQATKGQKYSVLGGIAG
                     IAGTILGGPGLGQLAQAPFAVYLLKFSREYETEADVLGSQIMANAGYDPRDLANMFRT
                     IEQQGGGGGGFLSDHPSPSDRYARINREADMLRVSNAGNYSRDFASIQERLRGYPRAQ
                     SMAEIARSGQRYPAGQTTSNYPENAPTGRVDYPSSRYQSYSIFNGGVQVGVPSNWRQI
                     GDGSSVWFVPEGGYGQYNGQPVFTHGVNFGVAQTSNGDLQRATDEFIRNLTQGNSGLR
                     LNSNYQRTTLSGRTALAATLRNLNEATGRTESVRLVTTQLRNGQLFYAIAVSPQGERN
                     FDGAFQNVFSSLRIND"
BASE COUNT         1986 a         1888 c         2181 g         1961 t
ORIGIN      
        1 gatgtgcgaa ttggggctga ctcgttagtt gcagcaggtt cactcgttag cccgggcact
       61 atcatcccgc cccgctcact cgtcatgggt tttccggcac gagtaaaacg tgaactaact
      121 gatgatgaag tgcggggtct gacagttttt tggaagaatt acgttgacta cacgcgagag
      181 tatcttgagg aggagcgacg ggggtaagaa atgaaggatg atcctttctt ctccgacttg
      241 gattagcagt aagattcatc cgtttggagg cgcgcgcatt ttcacttcag accgcatagc
      301 agttacgatc ggtctcaggt tccatcacat ctctaatgga gatacgtccg gttcgaaccg
      361 tggtcttaat caatttgtga tctacggcgg ttttttctgg ttcaagtaat gccttctgcg
      421 aagacacaac ctgcgcgcgg tatgcgcgat ttcttaccgg acgaagtccg taaacgcgag
      481 ttcgtcatca gtgtgattaa agaggtatac gagaaatatg gctttgagcc actcgaaacc
      541 ccggccgtag agaacattga gacactcaca ggcaagtatg gtgaggaagg caatcagctg
      601 atcttcaaaa tactgaaacg cggagtgcac gagagcacgg gagaagccga tctcgctttg
      661 cggtacgact tgacggttcc cctggcgaga gttgtagcgg agtatcgcga taaactacca
      721 aagttcttca agcgatatca gattcaacca gtgtggcgcg ccgatcgacc tgcccgcgga
      781 cgatttcgcg agttctttca gtgtgatgtt gatgtacttg gctcgcgatc cgtaactgtg
      841 gagtcagaga tatgtgctgc tgccaatgat gttttggtaa gacttggatt caatgacttt
      901 tgtatccgag taaatcacag aaagattttg accggaattc tgggtgtaag cggtgttcct
      961 cttgagaaac acgatctcgc actcgtggcg ctcgacaagc tggataaggt ggggcgcgaa
     1021 ggagtgcaga aagagtttga atcaagagca atcgaccccg ttagcggcga acgcttgctc
     1081 gacttttttg ccgaacttaa cgaattggac caagctgctg aaattgcagc cggggaagat
     1141 ccggagcgta aacagcaagc gctaaatcag gcagtgatcg gccgcttagt cgaattcgtc
     1201 ggtaataatg aagtgggtgc tctcggtgtt agcgaactgc aatcgattct tgattatgga
     1261 gaagcttccg ctattggtcc acgattgaaa attgatccca gtttggctcg aggtctttcc
     1321 tactacacgg gcgcaataat ggagatcaat gttaaggacc tgccgggaag tctcggcggc
     1381 ggcggccgct acgataacct tgttgggatg tttttgggcc aggatgttcc ggcatgcggt
     1441 ttctccctgg ggttggaaag aattctcgtc gttatgtcag agcgaggtat gtttccccct
     1501 gaggtaggaa ctacacctgc ggacattatg gtcacaatct ggaatgagga gtcagttgac
     1561 gactcgatcc ggctggcgcg agagttgcgc acaatcggcc tccgagtcga tctctacccc
     1621 gaagccgata agcttggtaa acaattcaaa tatgcgtcat cacgcggcgt gcctttagta
     1681 gccttgctcg gagatgacga gcgagcaaag ggagaagtcg cgatcaagga tatgagaacc
     1741 ggagaacaac ggacgtacaa gcgagccgaa ctggacacag ccatgatacg agcggtctaa
     1801 tgttcggaaa ggaggtgcga ttatggcgtc tgatgatgta gtgaaggatt atttgagcga
     1861 ttcaatcgcg atgtttaggt cttacaagaa gatggtcgac aatgccatca tccaggtaaa
     1921 tgatgatgaa ctctttgcaa ctatcgatga ggagtcaaat agtattgcca tcgtaatgaa
     1981 gcacttggcc ggcaacatgt tgtctcgctg gaccgacttt ctcactacag acggcgaaaa
     2041 gccaactcga aatcgagata tggagtttgt actcgagtct aaaaacaagg gcgagctact
     2101 ggattactgg gagcgtggct ggcgctgcgt ctttgatgcg ctggaaccct tgcaactcga
     2161 ggactttgaa aagacggtaa caattcgagg agaagagcac acgatagtta aagcaatcaa
     2221 ccgtcagctg tcgcattacg cataccatat cggtcagatt gtttttctcg caaagcattt
     2281 ccgttccagc gagtggaagt cgttgagcat accgaagaac aaatcggcag ctttcaatca
     2341 gtatctacag caaggcaaag gggcgggcca ggatcgaatg gacgccgcta tgacatttgc
     2401 aaaagatacc gatagccaat gatgagacct gagatcgtct tcagagatta gtctagttat
     2461 ggacaccaaa cttcatcacg tgaatgttac cgtacccaaa tcgttggaaa cggatgcaaa
     2521 gcacttctac ggcgtagtca tggggttaac agaggttccg aagcctgcgg agtcacgtgg
     2581 acgcggcgga gcgtggtatc aacttggccc aatgcagctc catctttcaa ttgaagatgg
     2641 attgggcgct gaatgtatca gcaagcgtca tgtgtgctat acggtccctg acctggcagt
     2701 ggcggagcat aagttcagag aagctggagt agaaattttg ccagacgata tcccgacgcc
     2761 tggttggacc aggttctatg tccgcgatcc aggtggtaat cggctggaga ttgctcagga
     2821 taattgatca ttaaccggta cgaatggaaa cacatatttg atgttggata atcttggcga
     2881 cctcgagcgc acgcattcat gtggacagtt gcgtgccagt gatatcggga aacaagtctt
     2941 attgatggga tgggtagcga agcgacgaga cttcggcgac ctgacctttg tagatctgcg
     3001 cgaccgcgac ggaattactc aggttgtttt taatgctgag tctgctgccg aggctcatac
     3061 aaaagcgaaa gacttaagga gcgagtacgt gatcgctctt aaaggcgaag tggttccacg
     3121 tgaagagaac actcgcaatc caaaactatt aacaggcgat gttgaagttc acgcgcgcga
     3181 actatttgtc ttaaacgacg ctcgtactcc gccatttcaa ctcgatgcct ctcctgaagg
     3241 tcttgcaagc gaagaccttc gtttgaagtt tcggtacctt gacctgcgcc ggccccagct
     3301 acagtcgaat cttcgcctca gacatcgggt cgtaactgaa attcgacgtt acatgagtgc
     3361 gcagggcttc acggaaattg agactccgat cttgatcaag tcgacacccg aaggtgctcg
     3421 cgactacatc gttccctcga gaattcagcc cggaaagttt tttgcgcttc cgcagtcgcc
     3481 acagctattc aagcaactgt gtatgatcgg aggcttagac aagtattttc agatcgcgca
     3541 ttgctttcgc gacgaagatt tgagggcgga ccgtcagcct gagtttacgc aactcgacgt
     3601 agagctgagt tttgcaacac tcactcaggt ctacgcatta atcgaaggat tatttgccag
     3661 cgtcttcaag ttaatcgacg tagagttgcc gacgccattt ccacgtctat cttttgaaga
     3721 ggcaattaga cgctttggtt cggacaaacc ggacctaagg attaagggaa tggagttgca
     3781 ggatattagt ggagtgttga cgagtaccga gttccctccg tttgcgtcgt ctcttgccgc
     3841 cggaggtcag attaaagcta ttgttgttcc gggtggtgca aacctttctc gaaaagtcct
     3901 ggacgacttg caggaatttg tgcgccggta tggcgccagt gctttggcat ggatcaagtt
     3961 gggcgacgaa acttcgtcgt cgctcttgaa agcgcttggg ccagatctcg tggcacgtat
     4021 cgcgcagaca actggtgcag cctccaacga tgcggtgttg attgtttccg gcaaggcgaa
     4081 agtcgttgca gcaagtttag gagcactgcg gaatgaggtt gctaagcgcg agcagttgat
     4141 tccacagaac gtctacgcgc ctttgtgggt taccgagttt ccaatgttcg agtaccacga
     4201 agaagatgga cgctactatt cgatgcacca tccatttacc tcacctgtgg atgaggatct
     4261 caatcttctc gaacgagcag tcgagggcgg ggaaacggag ttacttgcga agatacgtac
     4321 gaaggcttac gacccggtca ttaatggtgt tgagatggct agcggatcaa ttcgtattca
     4381 tcgtcgtgac gttcagcgtc tggttttcaa agcgctcggc atgactactg aagaggcgcg
     4441 cgagcggttt ggcttctttc tcgacgcgct cgaatatgga actcctccac atggtggcat
     4501 cgctcctggt atcgaccgat tgatgatggt gttggctggt actgacaata tgcgagacgt
     4561 gattgcgttc ccgaagaccg cgtcagctgc cgacctcatg atcgacgcac cgggtgaagt
     4621 ggattcgcga caactcgacg agctgcattt gaaagtagtt caagattaac taatcccctt
     4681 attatttttg gaaccgaaac ggagtggtgc tgtctaaact tacatgacgc acaagggctc
     4741 cgttcgagtt ctgctcttca ttggctcaaa tctgttactt ctttcttaca aggcttgtga
     4801 agagggcgac gctcacctgt cttattcatg cgtccctgtt agcgatgcct ctccaccgtt
     4861 tctcggcttt ggtgaatgga tcagcgtggg tatcttcctg tttgttgtgg catcaggcgt
     4921 tctagtgagt gccgtgatga tattaacgca gcactatcgc tgctcggtct acgagtcgat
     4981 aggactgcag cttgaaaccc accgaaaaat gtaaaattaa ggtctcaaat tccatatcaa
     5041 tggcgtaaac taatgacgca tggtggccct tcaactccgt cgcgaacatt ttctcgctct
     5101 ggtggaccat gcgcgcgaga ctgcgccagc tgagtgttgt ggactcctgg gtggaactga
     5161 gacggctaag gctgaaagta tctacaaact gcgaaacgtc accacgaacc ccgaaacagg
     5221 ttatgaagca gcacctgaag acttgtttgc tgcccagcgg caaatgagag atcgaggcga
     5281 agagctgttg gccatttacc attctcatcc gcgcgcttcg aatccgagcc cgtcggaaac
     5341 tgacgtgcgg ctcgcctact atcctgatgc aacatatttg attgttggct taggaggggc
     5401 tgaacctgtg gtcaaggcct ttcgtatttc tgagcgtgag cgaagttggc atccggttga
     5461 gtatgtgatc actggcgagt aagattggtg atgaatgatg gaaggtaata agagcaaata
     5521 cgacacaaac ccgctcgatc ccgacgttga acgtacgacg tctgaagctt ggggagacgg
     5581 ggcgagtgcc ggtcagacac agccagtgca gggaattacc cagaaggttg gcggatctca
     5641 ggaggacagt tcaaggaaga acgtttactc cgaggctcct acccgacgct acgatagccc
     5701 gcctctcgag acaccctacc cgtcagtatt tgtgccaccg acatattcgc cacctgttaa
     5761 acagcaattc accggaagtc catatcaacc tccaaggcaa agcagttcac ctgcacattc
     5821 agtatccggt tttggaatac ccgagcggtg ggcgacgatg ctggcgtata cgccgtccta
     5881 cataggtctg gttgtgtcct tgttggagtt gtttttagtt ccgcgaaatg aagtaaaagt
     5941 tcgctttcat gcttcgcaag caatcgcaat tcatattggc atcctgatcg tccaaacggt
     6001 atttgctgca attcatgcaa ttacaggcag ctcaatcgga gggactcttt tcggagtgct
     6061 ctcctttgtt ttcctgataa tctcaatgat caaggtgtgg cgtggtgact cccaccggat
     6121 tgagccagtt gcggaagcgg ctcaatggtt taaccaacat atcgagcccc gaaagcaggg
     6181 ttaatcccgc actggcatct tcgttgcatc tgtgaggccg aagggttaat tcgtaccctt
     6241 taaagcgagc gcgcgacgca aaacaggtct gataaactca ctttgtgttg cgatttggtg
     6301 cagctgcatg ccaaaagggt tacggactcg ttggcaaact cgctccctgg gacatcatca
     6361 aaataggaac gtcagaaact atcctggtaa ttgctggagg tcacgatgat caaacaaagc
     6421 aggtttattg catggctcgc agtggtggct attttctcaa tgccactttc agctgtggcg
     6481 cagaccccaa tcaaatatca cagcaacaag tatagtgttc aggacgatat aaagcttggg
     6541 cgtcaagccg cccaggaggc cgaacagcag tttccattat tgaatgacga gacagttcga
     6601 agttacgtgg cacgagttgg tgaccgtctc gttcgcgcaa tacccaatca gtttcaacat
     6661 ccggaattcc gatactactt taaagttgta aatgcgagtg acataaacgc gttcgccctg
     6721 cccggtggcc caatgtacgt caaccgcgga atgattcaag cggcgcggaa tgaaggcgag
     6781 atggccggcg tcatggccca cgagataagc cacgttgctc tccggcacgg tactgctcaa
     6841 gcaactaaag ggcagaaata cagcgtactg ggcgggattg ccggcatagc gggaacgatt
     6901 ctgggtggcc cgggattggg acagttggcc caggctcctt ttgcagttta tctactcaaa
     6961 tttagccgcg agtacgaaac tgaagccgac gtgcttggct cacaaatcat ggccaacgcc
     7021 ggatacgatc cgcgcgatct ggccaatatg tttcgaacca tcgaacagca gggcggtggt
     7081 ggcggaggct tcttgagcga ccaccccagt ccaagcgatc gttatgctcg aatcaatcga
     7141 gaagccgata tgctgcgagt aagtaatgct ggaaattatt cacgagattt cgcgtcgatc
     7201 caggaacgac tgcgtggata cccacgcgct caatcaatgg ccgaaattgc tcgtagtggg
     7261 cagcgttatc cggccggcca aacaacaagc aattaccctg agaatgcgcc gacaggccga
     7321 gttgattacc cctccagtcg ttatcagagc tactcaattt tcaatggtgg agtacaggtt
     7381 ggtgttccca gcaactggag acagattggt gatggcagtt cagtttggtt cgtgcccgaa
     7441 ggcggttacg gacagtacaa cggtcagccg gtattcactc atggcgttaa tttcggtgtt
     7501 gcccagacca gcaacgggga ccttcagcgc gcaacggatg aattcatcag gaatctgacg
     7561 cagggaaact cggggttgag attgaacagc aattaccagc gtacaacgct atctggaaga
     7621 actgcgctgg ctgcaacgtt gagaaattta aacgaggcta ctggacgaac cgaaagtgtt
     7681 cgtctggtga ctactcagtt gcgcaatggt cagctgtttt acgcaatagc tgtttcgcca
     7741 cagggtgagc gcaactttga tggcgcattc caaaatgtgt tcagctcgct gcggatcaat
     7801 gattgagaag tgttcgtagt ccgtagtccg ttgtcgggag tcattaatct gacggacaac
     7861 gaactgacaa ctgacaactg acaacggact acttcctctt tgcttcctgc cagagagctt
     7921 ccaaatcgtc aatcgaagtt tcatcaaatg attgtccacg ttcgtgcagt gtcttctcga
     7981 tgtagccgaa ccgtttgcga aatttccggt tagtca
//
LOCUS       DASSFP010000208         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1090101, whole genome shotgun sequence.
ACCESSION   DASSFP010000208 DASSFP010000000
VERSION     DASSFP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090101"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFH91_04505"
     CDS             complement(<1..935)
                     /locus_tag="VFH91_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HET6862279.1"
                     /translation="MRVLIADKFEQSGRDGLKALGCDVTYEPDLKDDLLVQAINSKEP
                     DVLVVRGTRVTEPMFEAGPIKLVVRAGAGYNTIDVAAASRRGIYVSNCPGKNAIAVAE
                     LAFALILAVDRRIADNVISLRRDEWNKKGFSKARGLFGRTLGLIGLGQIGLEMIPRAR
                     ALGMPVVAWSRSLNPDRATSLGIEFKASPLEVAKVSDILSVHVALNSETRNLINKEIF
                     DALQPGSYFINTARGEVVDQQALVEAMKTRGIRAGVDVYASEPTSSTGEFSDPIAHEE
                     NLYGTHHIGASTDQAQEAIAAETVRIVREFKETGQV"
     gene            complement(944..2170)
                     /locus_tag="VFH91_04510"
     CDS             complement(944..2170)
                     /locus_tag="VFH91_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HET6862280.1"
                     /translation="MAVIRPFRALRPPVERAKTVASVPYDVVNTEEAKALANGNELSF
                     LHVSRPEIDLPPGTDLYSDQVYRKAVENFQKLIEACPLQQEVEPSLYLYRLVMGEHQQ
                     VGIVGCCSIDEYDDNTIRKHERTRRDKEDDRTRHMVMLRAQTGPVFLTYRRRSEIDAQ
                     AAEILATEPLFNFTADDGVRHTIWRIADTGKLVSSFEAVPLLYIADGHHRAASASRAR
                     AELKEQSFGHTGNEDYNYFLTVLFPDSQLQILAYNRVVRDLNGLSKADFLAEVRKQFN
                     ISENVTPAPANRNQWSMYLEGKWYGLTLRPDTTLPAGTVAQLDVSVLQDRLLDPVLGI
                     KDIRTDKRIDFVGGLRGTKELERLVNEGKAAVAFSLYPTTVDELLMVSDAGEIMPPKS
                     TWFEPKLRDGLLIHLI"
     gene            2450..3154
                     /locus_tag="VFH91_04515"
     CDS             2450..3154
                     /locus_tag="VFH91_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6862281.1"
                     /translation="MNEDDAQKIRELELKVRAKSAEFDREFRARGFDPAQLENTAITG
                     SLSHIYEEREVLLTELEALKMATAKETREWTERERISDQFRRAFEGEAWHGPSVLALL
                     EGVTAEQAAAHPVAGAHSIWELVLHIEAWERACKRRLQGDRARLETLEDWPVVSDHRE
                     SAWEQTKQKLIETHRELVAAIGSLDESRLDKPIIDDPTTPYSSTYVTLHGGVQHDLYH
                     AGQIAILKKALEGVAS"
     gene            3177..3503
                     /locus_tag="VFH91_04520"
     CDS             3177..3503
                     /locus_tag="VFH91_04520"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET6862282.1"
                     /translation="MSELASRQCVPCRGGVPPLETEAINKLVSQLDQWEVVNDHHLRK
                     AYKFSDFREAQTFVNAVSDLSEEQGHHPDICFGWGKAEITIWTHKIDGLTESDFILAA
                     KIDRLD"
BASE COUNT          884 a          868 c          956 g          874 t
ORIGIN      
        1 acctgaccag tttctttgaa ttcacgtacg atccgcacgg tttcggccgc aattgcttcc
       61 tgggcctgat cggttgatgc accaatatga tgtgtgccgt aaagattttc ctcgtgagcg
      121 atcgggtcag aaaattcacc ggtgctcgag gttggttcag aggcgtatac gtcgacaccg
      181 gcacgtatgc cacgagtctt catcgcctca accagggctt gctgatcgac gacttcgccc
      241 cgcgccgtgt taatgaagta actaccgggc tgtagagcgt cgaagatctc cttattgatg
      301 aggtttcggg tttcagaatt cagggccacg tgtacgctga gtatgtcaga aacttttgcc
      361 acttcgagag gcgatgcctt gaactcgatt cctaacgacg ttgcacggtc cgggttaagg
      421 ctccggctcc acgcaactac aggcatcccc agcgcgcgag ctcgcggtat catttcgagt
      481 ccaatctgtc ctagtccgat taggcccaaa gttctgccaa agagtccgcg tgctttggaa
      541 aagcctttct tgttccactc atcgcgtctt aggctgataa cattgtccgc gatacgacga
      601 tccacagcga gtatcagcgc gaatgcgagc tctgcgactg cgattgcatt tttgccggga
      661 cagttcgaaa cgtagatgcc gcggcgagaa gctgcagcaa cgtcgatagt gttgtagccg
      721 gcgccggccc tgacgacgag cttaattgga ccagcctcaa acattggttc agtcacgcgc
      781 gttcctcgta ccacgagtac gtcaggctct ttgctattta tagcctggac cagtaaatcg
      841 tccttcaggt ctggttcgta cgtgacatcg cagccaagag ctttcaaccc atcgcgaccg
      901 gactgttcaa acttgtcggc aataagaaca cgcataaggc ttctcaaatt aaatgtatta
      961 gcagaccatc tctgagcttc ggttcaaacc aggtcgactt ggggggcatt atttcacctg
     1021 catccgaaac catcagcaat tcatcaactg tcgtgggata gagtgaaaat gccacagccg
     1081 ctttaccttc attaaccagc cgctccaact cttttgtgcc acgaaggccg ccgacgaaat
     1141 cgattcgctt gtcggttcgg atatccttta ttcccaaaac tggatcaagc agtcgatctt
     1201 gcaagacgct aacatccaat tgagcaaccg ttccggcggg cagtgtagta tcgggtcgaa
     1261 gagttagacc ataccatttc ccctcgagat acatggacca ctggttgcga ttagcaggag
     1321 cgggagtcac gttttcagag atgttgaatt gtttccttac ttcagcaagg aaatcagctt
     1381 tcgatagtcc attgagatct cgaacgacgc ggttgtaagc gagaatctgc aattgggagt
     1441 cgggaaacaa taccgttaga aagtagttgt agtcttcgtt gcctgtatga ccgaagctct
     1501 gttcctttag ttcggcgcgc gcgcgactgg cgctggcggc gcgatggtgg ccatcggcga
     1561 tgtaaagtaa tggcacagct tcaaagctgc tgacgagttt gccggtatcg gcaatgcgcc
     1621 agattgtatg tcgcactccg tcgtccgcgg tgaagttaaa gagaggttcg gtagcgagaa
     1681 tttctgctgc ctgtgcatcg atctcagatc gacgcctgta ggtcaaaaaa actggaccag
     1741 tttgagcgcg aagcataacc atgtgccgcg tgcgatcgtc ttccttatca cgtctggttc
     1801 gctcgtgttt tcggattgtg ttgtcgtcgt attcatcgat agagcaacaa ccgacaatcc
     1861 caacctgttg atgttcaccc ataacgagtc gataaagata caggcttggc tcgacctctt
     1921 gttgcagagg gcacgcttca attagtttct gaaaattttc tacggctttg cgataaacct
     1981 gatcggagta caaatcggtg cctggcggca aatcgatctc agggcgcgag acgtggagaa
     2041 agctcagttc gttaccattc gcgagggctt ttgcctcttc agtatttacg acatcatagg
     2101 gaacactggc cactgttttg gcgcgctcga ccggcgggcg caaagctcgg aaaggacgaa
     2161 taaccgccac ggaacctcct cttgaacaaa cgcacaatca ggaccgcgta gcttgcaagt
     2221 tctgatcaat taggtgctgg tgaaaagtgt aaaggcttat gcgggtaaga ctactgatag
     2281 tacctgcagt tcgcaagtat tgggcgcctt accgatgcca ctatcacgga accagcggcg
     2341 aagcgactgc atgccatcct ccatacttct gtggttgcgt atagtcgctt cggtcctggt
     2401 tccctactat tgcacatggg ggtgctaaaa cggttagaat cgtgctcaaa tgaatgaaga
     2461 cgatgcgcag aagattcgtg agttggagct caaagtgcgc gcaaaatccg ccgagtttga
     2521 tcgagagttt cgggctcgag gttttgatcc ggcgcaactt gaaaacacgg cgataaccgg
     2581 ctctctcagt cacatttacg aggaaagaga agtgttatta accgagttgg aggctttaaa
     2641 aatggcaaca gcaaaggaaa caagggaatg gacagaacgg gaaagaatta gcgatcaatt
     2701 tcgacgagcg ttcgaaggcg aggcctggca tggaccatca gtgctggctc tgctcgaagg
     2761 cgtgacggct gaacaagcgg ctgcgcatcc ggtcgcaggc gcacattcaa tctgggaatt
     2821 ggtgcttcat atcgaagcgt gggagcgtgc ttgcaagcga cgcttacaag gtgatcgggc
     2881 gcgattggaa actctggagg attggcccgt ggtgtctgac catcgcgagt cagcatggga
     2941 acagaccaag cagaaattaa ttgaaacgca ccgggaatta gtcgcagcga tcggatctct
     3001 ggacgaatcg agactcgaca agccaatcat cgatgatccg acgacgcctt attcgagcac
     3061 ctacgtaaca cttcatggtg gtgttcagca cgacttatat cacgccggcc agattgcaat
     3121 ccttaagaag gcgcttgaag gggttgcatc gtagaagtag ttctggagta aacattatga
     3181 gcgaattggc tagtcgacaa tgtgtgccgt gtcgcggtgg agtgccgccg ttggaaactg
     3241 aggcaatcaa taaattggtt tctcagttag accagtggga agtggtaaac gatcatcatt
     3301 tgaggaaagc ctataagttt tctgactttc gcgaagccca gacttttgta aatgcagttt
     3361 cggatctgtc agaagagcaa ggacaccatc cggacatttg cttcggctgg ggcaaggcag
     3421 agataactat ctggacgcac aagattgatg gtctaactga gagtgacttt atactggcgg
     3481 caaagattga taggttggac tgagcatttt ccatagatca ttttccattt ctcatttttc
     3541 atctgcttac aaacaagcgc ccgacaaaaa tgaaaagtga ag
//
LOCUS       DASSFP010000209         7128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1090285, whole genome shotgun sequence.
ACCESSION   DASSFP010000209 DASSFP010000000
VERSION     DASSFP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090285"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFH91_04525"
     CDS             complement(<1..755)
                     /locus_tag="VFH91_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6862283.1"
                     /translation="MKRQIRIIALIAALTFSVSNGLAQTPQPLDRTKIPPPGPAPMLH
                     VPTWTKSQLANGALMIVSERHNLPLVSFTITFVGGSNQFEPVAKRGLANMTSSMLTEG
                     TTTKSGDQLSDALQLLGTNVNANIGGEEGSIGFVSTTGNFEPTLAILTDMMLNSTFPA
                     EALERLRGRTLVGLTQAKDQPTVVGAQVFAKILYGDAHPYGQRATEASIKAISRDDIV
                     AFEKAYFQPGRAIITVVGDISPIKAKSVVEKTLA"
     gene            complement(755..2188)
                     /locus_tag="VFH91_04530"
     CDS             complement(755..2188)
                     /locus_tag="VFH91_04530"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6862284.1"
                     /translation="MITERRIALVVALFLSLCCSFQTSLAQSKAGASNAIKIPYETYK
                     LPNGLNVILSVDRTTPTVAVNIWYHVGSKNEMAGRTGFAHLFEHVMFTGSGHVPYGTH
                     DKLTEGVGGNNNGSTTNDRTNYYENIPSNYLESALWMESDRMGFLLDTLDIAKLNAQR
                     DIVKNERRQSYDNQPYGRVGEIFAAAMYPAGHPYSWPVIGSMTDLSAASEEDVKAFFR
                     LYYAPNNATIAIVGDFDPAQAKAWVTKYFADLPQGKQVQRPSVPLGKLQASKRLVYED
                     SVQVPRLYIQWPSVGVKSDDDYALSVMGSILSGSRTARLTKALVYDTQMATQVGAFQR
                     SNEDVGEFQLVVIPRPDHSLTEIEDAADQIIKKFISEGPTAEELQKAKSGLELGFVRG
                     LESNLGKANQLLDGAVFHDNPGYFSTDYQKTLAVTAADVKRVAAQYLGGNRIVLSVVP
                     KGKKDQASKATESEIVSVLLNTLRGER"
     gene            2457..3425
                     /locus_tag="VFH91_04535"
     CDS             2457..3425
                     /locus_tag="VFH91_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET6862285.1"
                     /translation="MQAIQVSQVGGPEALQLAELPVPKPKPNEAIVQLKASGVNFIDV
                     YFREGRYPTPLPFVDGQEGAGVVTDIGSEVTNVKVGDRVSYTGVPGSYAQYAAVPSVR
                     LVHIPDDVSFDLAAAVMLQGMTAHYLLNSTYKLQSGEVALIHAAAGGVGLLLVQLAKR
                     AGARVIATAGSPDKTELAREAGADECINYNEQDFEVETRRLTDGQGVHVVYDGVGKAT
                     FDKDLNVLRPRGYLVLFGGSSGAVPPFDLIKLSQKGSLYVTRPTLANYIATREDLEWR
                     SKEILDLVGSGELKVRIHHSYPLADAAQAHRDLEGRKTTGKLLLKI"
     gene            3714..4037
                     /locus_tag="VFH91_04540"
     CDS             3714..4037
                     /locus_tag="VFH91_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET6862286.1"
                     /translation="MNIEHWRKEIDEIDDEMLRLLNMRARLAIKVGALKKQAQLPLCD
                     PVRERSVLLRLSQLNQGPLDERAIRKVFRRIIRESRRLEVTIDNTDGLANAAAEAHQS
                     VRGIL"
     gene            4034..4642
                     /locus_tag="VFH91_04545"
     CDS             4034..4642
                     /locus_tag="VFH91_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HET6862287.1"
                     /translation="MNSEKKRIAIQGERGAFSEEAAIALLGEDIELVPRQTFASLYSS
                     FEDQVADYLIAPIENTIAGIVEASVQRLRASSLVIIDEVSIPIAQNLIGLPGAKFSDI
                     KVVQSHPVALAQCALFFARHPSLKRLEADDTAGSVAQIIQRGDQTYAAIAGRLAAEVY
                     GASILRENIQDIAENYTHFVLLAQAAQRAATAQTKNNKENVS"
     gene            4639..5655
                     /gene="aroF"
                     /locus_tag="VFH91_04550"
     CDS             4639..5655
                     /gene="aroF"
                     /locus_tag="VFH91_04550"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6862288.1"
                     /translation="MIVNMSNSATPEQINHVIERIKECGFQAHVIQGEERTIIGAVGS
                     GERRSELEALRAAPGVAEVIQIAHPFKLVSRQLRQTPTVVDVRGRKIGNGEVVVIAGP
                     CSVESQEQLLETAHAVKAAGASMLRGGAYKPRTSPYDFQGLGVEALRFLDQARKETGL
                     PIVTEVMSESDVEVVAEYADMLQVGARNMQNFSLLRKLATAPKPILLKRGPSATVKEW
                     LLAAEYLLAGGNGNVVLCERGIKTFETATRNTLDLAAVALVKELSHLPVLADPSHGTG
                     LRSLVTPMSKAAVAAGADGLIIEVHPCPERALSDGPQSLDLTSFASLMKELQKEPLRD
                     VKAA"
     gene            complement(5707..>7128)
                     /locus_tag="VFH91_04555"
     CDS             complement(5707..>7128)
                     /locus_tag="VFH91_04555"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HET6862289.1"
                     /translation="RLLQQSTARVKHALWVSGLLLCVAVPMLTSVASAIQLPVWRATP
                     KQVSIGELADPHRIDQTGAGITQLLPEMSSRLVLNRTLGLSVLSVFLLISAYQTLKLV
                     KAWWLTRKIKTFGFKANLSTNVEAILDRCRQSLPARFERPVDVLFSESVSVPITLGLI
                     HPKIILPAELLNEASDELLTSAIGHEFVHVARRDYFLNLVYEVLYVPISFHPAAALMR
                     RRVKQTRELRCDELVASEILKPEVYARSLVALAGSAPSTGRLSPITTVGIADADILET
                     RIMSLLKKSEPNHRWKRIPLLVSVILLVVPCIAAASLAMKFDVAPELSPVAQDPVKQS
                     KERTDKEQAERMRVTFDEREMKARMESDPQFREEVMRKREVEVEMRAIKQAALVKLAR
                     INMDQAIQIAGSQYPGKVLQCSLDAEKWEEPGKLAADGIVFYRVVVASDDPEAGLTHV
                     WVNAVDGSIIKTEREIPRKKRAQ"
BASE COUNT         1744 a         1868 c         1823 g         1693 t
ORIGIN      
        1 gccagggtct tttcgacaac gctttttgct ttgatcggac taatgtcacc aactacggtg
       61 ataatggcac gacccggctg aaaatacgcc ttctcaaagg caacaatatc gtcgcgcgaa
      121 attgctttga tagaggcctc ggtagctcgt tgtccgtaag gatgtgcgtc accataaaga
      181 atttttgcga aaacctgcgc gcccaccaca gttggctggt ccttagcctg cgttagacca
      241 acgagcgtcc gacctctcag gcgctcgaga gcctctgcag gaaacgtgga gttcagcatc
      301 atatcagtca ggatcgcgag agttggttcg aaattaccgg tggttgagac aaatccaatc
      361 gagccttctt caccaccaat gttggcattg acgtttgtgc caagcagctg caacgcgtct
      421 gacaactgat ctcccgactt ggtggttgta ccttcagtca acatggaact ggtcatattt
      481 gccaatcccc gctttgcgac gggctcaaac tgatttgaac cgcccacgaa agtaatggtg
      541 aaagacacca gcggaagatt gtgtctttca gagacaatca ttaaggcacc gttggccagc
      601 tgtgacttgg tccaggtcgg cacgtgcaac atcggcgccg gtcccggagg aggaatcttg
      661 gttcggtcga gaggctgtgg cgtctgcgcc aggccgttcg agacactaaa agtcaacgcc
      721 gcgattagag cgataattcg tatttgtcgt ttcattatct ctctccccga agagtgttaa
      781 ggagcacgct gactatctca ctctccgttg ctttggaagc ctgatccttt ttgccctttg
      841 gcacaacgct caggacgatg cgatttccgc cgaggtattg agcagctact ctctttacat
      901 cagcagccgt aacggccagc gtcttctggt agtccgtact gaaataaccc ggattatcat
      961 ggaacactgc cccatccagc agctggtttg ctttcccaag atttgattcc aatccgcgaa
     1021 cgaagccgag ctcaagtcca cttttggctt tctgaagctc ttcggcggtt ggtccttcgc
     1081 tgatgaactt tttaattatc tggtccgccg cgtcttcaat ttccgtgagc gaatgatcgg
     1141 ggcgcggaat tacaaccagc tgaaattctc cgacgtcttc attcgaccgt tgaaaggctc
     1201 cgacctgcgt tgccatctgc gtgtcgtaga ctagcgcctt tgtcaggcgt gcagttcgcg
     1261 aacccgaaag tatcgaaccc atcaccgaaa gagcgtagtc atcatcactc ttcacaccaa
     1321 cgctgggcca ctgaatgtat aggcgaggca cttgtacgct atcttcgtaa accagacgct
     1381 tactggcctg aagttttccg agcggtactg aaggtctttg cacctgtttg ccctgcggca
     1441 ggtcagcgaa atatttcgtg acccacgctt tcgcctgagc cggatcgaag tcgccgacaa
     1501 tcgcaatggt tgcgttgttc ggagcgtaat agagtctgaa aaacgctttg acatcttcct
     1561 ctgaagcagc gctcaggtcg gtcatcgaac caatgaccgg ccacgaatag ggatgaccag
     1621 ccggatacat cgcggctgca aatatttcgc ccacgcgacc gtaaggctga ttatcatagc
     1681 tctggcgtct ttcattcttg acgatgtcac gttgagcatt gagtttcgca atgtcgagcg
     1741 tgtccagcaa gaagcccatg cgatcagact ccatccagag tgctgactcg agatagttcg
     1801 aagggatgtt ctcgtagtaa ttggtccggt cgtttgtcgt tgatccgtta ttattgccac
     1861 ccacaccttc ggtcaactta tcgtgggtgc cgtaaggcac gtgacccgaa ccggtgaaca
     1921 ttacatgttc aaacaggtgc gcaaagccgg ttcttccagc catctcattt tttgacccaa
     1981 cgtgatacca gatgtttacc gccacggttg gcgtagtgcg atcaaccgag agtatgacat
     2041 ttaaaccgtt aggcagtttg taagtctcgt aaggtatttt gatggcgttg gaagcaccgg
     2101 ctttgctttg ggctaatgac gtttgaaacg aacagcaaag acttaagaac agtgcgacaa
     2161 caagcgcaat tcttcgttca gtaatcacaa cgactccttt cgcgtggctg cgtgattttc
     2221 caggctggct aggcgtgcgg ccgtatccta tgaaggtgca agaatagtgt caactcattt
     2281 gaatacaaac gaatctagcg ctcagattcc tggagttcga tccaattcat tggaacggta
     2341 tgaaggcgac ctcagtccgg cgcccattcg aatcaaggat tcgctaacat ccgctgttgt
     2401 atcggctggc gcgcagcaga gttcactatt ccgacagcag gaattactat agggccatgc
     2461 aagcaataca agtctctcag gttggcggtc ccgaagcgct acagcttgcg gaattgcccg
     2521 tcccaaaacc aaaacccaac gaagcaattg tccaacttaa agcgtcaggt gtgaacttca
     2581 tcgacgtgta cttccgagag ggaagatatc ccactcccct tccgtttgtg gacggacagg
     2641 aaggggctgg cgtcgtcacc gatatcggct ccgaagtgac caacgtcaag gttggcgatc
     2701 gcgtttcata tacgggagtc cccggaagct atgcacaata tgccgctgta ccttcggtcc
     2761 gcctggttca catacctgat gatgtttcgt tcgatctcgc cgcagccgtc atgctccagg
     2821 ggatgaccgc acattacctt cttaacagca cttacaaatt gcaatcaggc gaggttgcct
     2881 taattcatgc tgccgccgga ggtgtcggtc tactcctcgt gcaactggcg aaaagagctg
     2941 gagcccgcgt gattgcaacg gccggaagtc cagacaagac agagctcgcg cgtgaggcgg
     3001 gtgccgacga atgcatcaac tataacgagc aagacttcga agttgaaacc agaaggttga
     3061 ccgatgggca aggagtgcac gttgtctacg acggcgttgg taaggccact ttcgacaaag
     3121 acctcaacgt tctgaggccg cgagggtatt tagtcttgtt tgggggctcg agcggcgctg
     3181 tcccgccgtt tgaccttatt aagctttccc aaaagggctc tttgtacgtg actcgaccga
     3241 ctctggcgaa ttacattgct acgcgagaag atttggagtg gcggtcgaaa gaaattctgg
     3301 atctggtagg gagtggagag ctaaaagtgc ggattcatca tagttatccg ctcgccgacg
     3361 ccgcccaggc ccaccgcgat ctcgaaggaa gaaagaccac cggcaaactc ctattgaaga
     3421 tctaattttg gtacccacgc caaaaatttc ttgacaccgg tttttgacag tgcctatagt
     3481 agccgtcggt catgaatacc actcgcacat ttttctactt cgcttttacc taccgctact
     3541 tcttttttaa ggagagcgga cggcgaagtt cgaaattgat gtgctgatga cccaataaac
     3601 taagcacaaa ttttcaaaca aaaccggccg cgaaccagct aattcgcggc cgttttgttt
     3661 gtttgtctct ttgaccaggc gggcacggaa attcaacccg ggagtcgcat taaatgaaca
     3721 tagaacattg gcgaaaagag atagacgaga ttgatgacga gatgttgcgc ttattgaaca
     3781 tgcgggcgcg tttagcaatc aaggttgggg ctctaaagaa acaagcccag ctgccattgt
     3841 gcgatcccgt gcgggagcgc tcggtactac ttcgccttag tcaactcaat caaggaccac
     3901 tcgatgagcg cgccattagg aaggtattcc gccgtattat ccgcgagtcg cgccggctgg
     3961 aagtgaccat cgacaacacc gacggcctcg ccaatgcggc agccgaagcc catcaaagtg
     4021 tgcgaggtat tctgtgaact ccgaaaagaa aagaatcgca attcagggag aacggggcgc
     4081 tttcagcgaa gaggctgcaa ttgctctact gggcgaggac atcgaactag tgccccgtca
     4141 aacatttgcc tcgctctaca gcagttttga ggaccaggtc gctgactact taatcgcacc
     4201 gattgaaaat acgattgccg gaatcgtcga ggcttccgtc caaaggttga gagccagctc
     4261 actcgtaatc atcgacgagg tgtcaatccc tattgcccaa aacctgatcg gattgcctgg
     4321 cgccaaattc tccgacatca aggtggtcca gtcacatcca gtagcactgg cccaatgcgc
     4381 attgtttttc gcccgccatc cgtcgctaaa aagattggaa gctgacgata ccgccggcag
     4441 tgttgcgcaa ataatccaac gtggcgatca aacgtatgcc gccatcgcgg gcagacttgc
     4501 cgccgaagtc tatggcgcgt ccatccttcg cgaaaacatt caagacatcg cagaaaacta
     4561 tacccatttc gtgttgctcg cccaggctgc ccaacgcgcg gcaactgctc aaactaagaa
     4621 caataaggaa aacgtatcat gatcgtaaat atgtcaaact cagctacacc cgaacaaatc
     4681 aatcacgtta ttgagcgaat aaaagagtgt ggctttcagg cccacgtaat tcagggcgaa
     4741 gaacggacca tcatcggcgc tgttggcagc ggcgagcgtc gttctgagct cgaagcattg
     4801 cgagccgcgc cgggtgttgc tgaggtcatt caaatcgcac atccattcaa actcgtcagc
     4861 cgtcaactca gacaaacccc cacagttgtc gacgttcgag gccgcaagat cggcaatggc
     4921 gaagtggttg taatcgcagg accatgttcg gtcgagtctc aggagcagct tctggaaacg
     4981 gctcatgcgg tgaaggccgc cggagcaagc atgttgcgag gtggcgctta taaacctcga
     5041 acttctcctt acgactttca aggactcgga gtagaggccc tgcgttttct ggaccaggca
     5101 agaaaggaaa ctggtcttcc gatagtcacc gaggtgatga gcgagtcaga cgtagaagtc
     5161 gtagccgagt atgctgacat gctgcaagta ggggcgcgca atatgcaaaa cttttcgtta
     5221 ctgcgaaagt tggcgactgc tccaaaaccg attttgctta agcgcggtcc atctgcaaca
     5281 gttaaagaat ggttacttgc cgccgaatat ctgttggctg gtggtaatgg caacgtggtt
     5341 ttatgcgaac gtggcatcaa gacctttgaa acagccactc gtaacacgct cgatttagcg
     5401 gcggtggcat tggtcaaaga gctgtcacat ctgcccgtgc tggcagatcc atcgcacggc
     5461 acaggcttgc gcagtttagt gactccaatg tctaaggcag cagtggcggc aggtgcagac
     5521 ggattgatca ttgaagttca tccgtgtccc gagcgagcac tatcggatgg cccacagtcg
     5581 ctggatttga cgagctttgc gtcattgatg aaagaactgc aaaaagagcc attgagggat
     5641 gtcaaagcgg cttaaagaga gcgttaggac cgataagacc taattggcct tattggtcct
     5701 attgccttat tgagctctct tcttccgagg tatttccctc tcggtcttaa taatgcttcc
     5761 atcgaccgca ttcacccaaa cgtgcgtcaa gccagcctca ggatcgtcgc ttgccaccac
     5821 tacgcggtaa aacacgatcc catcggcagc taacttccca ggctcttccc atttctcagc
     5881 atcgagtgaa cattgcaaaa ccttgccagg atactggctg ccggcaattt gaattgcctg
     5941 gtccatgtta atccgcgcta atttcacgag tgcggcttgt ttgattgcac gcatttcaac
     6001 ttcgacttcc cgctttcgca tcacttcctc gcgaaactgg ggatcactct ccatgcgggc
     6061 tttcatttcg cgctcgtcga acgtaacacg catgcgctcc gcctgttcct tatctgtgcg
     6121 ctcttttgat tgtttgactg gatcttgagc taccgggctt agctcaggcg cgacgtcaaa
     6181 cttcatagca agtgacgcgg ctgcaataca tggcacaaca agcagtatta ccgagactaa
     6241 caagggtatc cgtttccagc gatgatttgg ctcagacttt ttcagtagtg acatgattct
     6301 agtctccaga atatcggcat cggcgatgcc tacagttgta atcggggata aacgacctgt
     6361 ggacggcgcg gatcctgcca atgcgacgag tgaacgggcg tagacttccg gttttaagat
     6421 ttcgctcgca accagctcat cgcagcgcaa ctctctagtc tgtttgacgc gacgtctcat
     6481 caaagctgcg gcgggatgaa atgagatcgg cacgtacaga acctcataaa ccagattcag
     6541 aaagtaatcc cgccgtgcaa catgcacgaa ctcatgtcca atcgcggaag tgagaagctc
     6601 gtcagaagct tcgtttaata attccgcggg caagataatc ttcgggtgaa tcaaacccaa
     6661 cgttatcggc actgaaaccg actcggaaaa cagaacatcg actggacgtt cgaaccgggc
     6721 cggcagcgac tgcctgcagc gatccaggat cgcttcaacg ttagtgctta aatttgcttt
     6781 aaagccaaaa gttttgattt ttctggtaag ccaccatgcc tttaccagct tgagagtttg
     6841 atacgcagag atcagaagaa agaccgatag aactgataac cccagggttc tattcaagac
     6901 taaccttgaa ctcatctcgg gcaagagctg agtgatacct gcgcctgtct gatcgatgcg
     6961 atgtggatca gcgagctcgc caattgacac ttgcttcggt gtcgctcgcc aaactggcag
     7021 ctggatggca gaagctaccg acgtcaacat tggaacggca acgcagagaa gcaagcccga
     7081 aacccacaac gcgtgtttta cgcgggccgt tgactgttgc aggagcct
//
LOCUS       DASSFP010000210         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_353620, whole genome shotgun sequence.
ACCESSION   DASSFP010000210 DASSFP010000000
VERSION     DASSFP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353620"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFH91_04560"
     CDS             <1..634
                     /locus_tag="VFH91_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET6862290.1"
                     /translation="ARLCYALNIDTWEVIRAAATKPFGFMAFYPGPGIGGHCIPLDPH
                     YLSWKARQHGFDSRFISLAEEVNSRMPEHVVQLVADGLNDDTKAMKGSKVLLLGVAYK
                     KDIDDVRESPALSIIDRLRAKGADVRYHDPFVSEIRFHDAHTDSAGEPLRSIALTDEA
                     LNSADCVIIVTDHSEIDYKRVCSLAPLIVDTRNALNGELRQQSSARIIRL"
     gene            662..1768
                     /gene="wecB"
                     /locus_tag="VFH91_04565"
     CDS             662..1768
                     /gene="wecB"
                     /locus_tag="VFH91_04565"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET6862291.1"
                     /translation="MLKCINVVGARPNFMKVAPIVAAMKQRASRFNSLVLHTGQHYDA
                     MMSDAFFVDLELPQPEIHLGVGSGTHAAQTAAIMERFEPVVLEEKPDWVLVVGDVNST
                     IACALVCVKLGIKVAHVEAGLRSRDRTMPEEINRLLTDQIADLLLTPSPDADVNLKNE
                     GIPAERIRFVGNVMIDSLFKNLERARKSNIKAELGLSGKDYSVLTLHRPANVDVREPF
                     ERILDALQNISAQLPVIFPAHPRTRKTIADLGLESRLASLPNVRIIDPLGYLDFLALY
                     STARLVLTDSGGIQEETTSLGIPCLTLRENTERPITIEYGTNVLVGTDTTLIISEANK
                     ALNGPSRRVNHSIPLWDGHAAERILDVLEEVSPT"
     gene            1777..>2790
                     /locus_tag="VFH91_04570"
     CDS             1777..>2790
                     /locus_tag="VFH91_04570"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HET6862292.1"
                     /translation="MSFVRKLKRAIRGDISPTTATLEILRRTDVAIKARNERNSLARE
                     SKQPAPLCRPYSELSNSKLLEHFQTRQVPRSLPGLKLVPGADRRETNGVEGLIQYASQ
                     IVNEHCWDVLGFGTRSFADGIDWNRDLLSGYEWPLTYHRTLTFLRNDGSDARVVWELN
                     RLGHLVPLAGAYAITRDQRFLREFVTQLDSWREQNPYGYGVNWACAMEVALRAINLLA
                     AFEIFRSAPELTSVDLANILNLLQLHGRYIRRNLEYSYIATSNHYMTDVVGLVWLGVL
                     LPEFAEAVRWRDFGLREMLREIDKQILPDGADFEASTGYHRLILELLLYTFILCREND
                     VEIP"
BASE COUNT          660 a          717 c          726 g          687 t
ORIGIN      
        1 ggccaggctt tgttacgctc tcaatatcga cacgtgggaa gtgatcaggg ctgccgcgac
       61 taagcctttc ggattcatgg ccttttatcc tggaccaggc attggtggtc actgcattcc
      121 actcgatcca cactatttgt cctggaaggc tcgccagcac ggattcgact cgcggttcat
      181 aagccttgct gaggaagtta actcacgcat gccggaacat gtggtccaac tcgttgccga
      241 tggtcttaat gatgacacta aagcgatgaa aggctcaaaa gtgttactcc tgggcgtcgc
      301 ttacaagaaa gacatcgacg acgtgcgcga gagtccggca ttgtccatta tcgatcggct
      361 gagagccaaa ggcgcagatg tgcgatatca cgacccattc gtgtctgaga tacgttttca
      421 cgatgcgcac accgactcag ccggcgagcc acttaggtcg atagcactga cagacgaggc
      481 tctcaattcg gcggattgcg tgatcattgt caccgaccac agcgaaatcg attacaaacg
      541 agtctgttcg ttggctccgt taattgttga tactcgaaat gctctaaatg gagagctgcg
      601 gcagcagagt tcagcaagaa taatcagatt gtagtctggg agcctttcag acccgccaac
      661 catgctcaag tgtatcaacg tcgtaggcgc tcggcccaac ttcatgaaag tcgcgccgat
      721 cgtggccgcc atgaaacagc gagcgtcgag attcaactct ctcgtgctcc atacaggtca
      781 acactacgac gcgatgatgt cagacgcatt ttttgtagac ttggagcttc cccaaccgga
      841 gattcatctc ggggttgggt caggcactca cgcggcgcaa acggccgcca taatggaacg
      901 gttcgaaccg gttgtgctcg aagaaaagcc cgactgggtg ctggtcgttg gcgacgtaaa
      961 ctcgacgatc gcatgcgctc ttgtttgtgt gaagctgggc attaaggtcg cacacgtgga
     1021 agccggttta cggagtcgcg atcggaccat gcctgaagag atcaatcgtt tgctcacgga
     1081 ccagatcgcg gatttgctgc tgactccctc tcccgatgcc gacgttaact tgaaaaatga
     1141 agggattccg gcagagcgta ttcgttttgt cggtaacgtc atgatcgatt ctctcttcaa
     1201 gaatcttgag agagctcgaa agtcaaacat aaaagccgag ctcggtttgt cgggcaaaga
     1261 ttattcagta ttaacacttc atcgccccgc gaacgttgat gttcgagagc cctttgaacg
     1321 cattctcgac gctctccaga atatcagcgc gcagttgcct gtgatctttc cggcgcaccc
     1381 tagaactcgt aaaacgattg cagatttggg cctggagtcg cggctcgctt ctcttcctaa
     1441 cgtgcgcatt atcgatccgc ttggctatct cgacttcctg gccctgtaca gcacggcgcg
     1501 actcgtgctg acggactctg gcggcatcca ggaggagaca acctcgttgg gtattccctg
     1561 tctaaccttg cgagaaaaca ctgagcgccc aataacgatt gaatacggca caaacgttct
     1621 cgttggcact gataccacgt tgatcatcag tgaggcgaac aaggcgttga atggaccatc
     1681 caggcgggtc aatcacagta ttccgctctg ggatggacac gctgccgaaa gaattctcga
     1741 tgtgcttgaa gaagtttccc ccacctaagc cttttcatga gtttcgttag aaaattgaag
     1801 cgagcaatac gaggggacat cagtccgacg actgctactc tcgaaatcct tcgtcgtacc
     1861 gacgttgcta taaaggcccg taacgaacga aactctttag cgcgggaatc gaaacaacca
     1921 gcaccacttt gccggccata ttctgagtta agtaattcaa agcttcttga gcattttcaa
     1981 acacgtcaag tccctcgatc attaccagga cttaagctcg tacccggcgc cgatcgacgt
     2041 gaaactaacg gtgtcgaggg cctgatccaa tacgcttcgc agatcgtaaa cgaacactgc
     2101 tgggatgtgc ttgggtttgg caccagatcc tttgctgatg gcattgactg gaatcgcgat
     2161 ctactttccg gatacgagtg gccgttaact taccatcgca ctctcacgtt cttgcgcaat
     2221 gatggttccg acgcgcgtgt tgtgtgggag cttaatcgcc tgggccacct ggtcccactg
     2281 gccggtgcat atgcgatcac cagagaccaa cgctttctga gagagttcgt cacgcagttg
     2341 gatagctgga gagaacagaa tccgtacggt tacggagtta actgggcttg tgcgatggaa
     2401 gttgcgttgc gtgcaatcaa cttgttggct gcgtttgaga tctttcgttc agcacctgaa
     2461 ctaacgtccg tcgatttggc aaacattctg aacctgcttc agttgcacgg ccggtacatc
     2521 cggcgaaatc ttgagtattc gtacattgca acaagtaatc actacatgac tgatgtagta
     2581 ggtctggtgt ggttgggagt tttacttcct gagtttgccg aagcagtacg gtggcgcgat
     2641 ttcggattac gcgaaatgct gcgtgaaata gacaaacaga tcttgcctga cggggcggac
     2701 tttgaagcct caaccggtta ccatcgatta attctcgagt tgctcctcta tacttttatc
     2761 ctgtgtagag aaaatgacgt tgagatcccc
//
LOCUS       DASSFP010000211        10424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_207834, whole genome shotgun sequence.
ACCESSION   DASSFP010000211 DASSFP010000000
VERSION     DASSFP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207834"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFH91_04575"
     CDS             complement(<1..279)
                     /locus_tag="VFH91_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862293.1"
                     /translation="MRDLRRLLKYLRPHWAIFTLASISMVMVGILDTAIGGLIVPIFE
                     QALARDGGHRTSTIFGLQRLIPEAPFGALKMISVLLIVFTLGKGVAEYL"
     gene            522..1253
                     /locus_tag="VFH91_04580"
     CDS             522..1253
                     /locus_tag="VFH91_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6862294.1"
                     /translation="MAQQAQLNSKYAGKRLLIADDDTDMRLLLAEYFRRLGFQVEEKE
                     SGLAALESAMANRFDCFIFDVSMPGMSGLELLKRVRDRGVQTPTLFLTAHDALADKVA
                     GFEAGADDYLAKPFSPRELEYRVEALLRRGGVVTTDTTDEERIEVGDLIIDKRRHEVL
                     REGARIDLTPLEFQILELLASEPGRAWSRNALLDRVWSTDYEGYQRNIDPHINRLRKK
                     LETDPKNPRYVLTVRGVGYKLNEAP"
     gene            1600..3171
                     /locus_tag="VFH91_04585"
     CDS             1600..3171
                     /locus_tag="VFH91_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6862295.1"
                     /translation="MSLTLSHRKLALVAVICLLCSAFVFAQSDVERKTVAITYPLDET
                     VTVKFRGTTLLPRLKGEAKVKRAGRRGTRVELKIDDLPRASELGGIYTTYVLWAISPD
                     GQVDNLGEIKRSGSSFVDSKLDVTTPLQTFALILTAEPHFLMKVPSRMVVMENLPPTR
                     PNGSQIATADVRYIGNSSDYFRDARVPGIADADYKQTPVSLLGARQAVNLAKYAGASQ
                     DAPDELRIAEEQLQSAEKKWRLNQPVVEIDMEARSATSAGAHAEEVAYARKAARLQRD
                     EIRRRDEAVRSAEKTADESQREVSQLRAALEKEQRARELAERDAGTANDQLREKRVEI
                     AQLRDELQTLRSESESAKLKLARLEGEKQAETDRVEAERRAQERRAAEANLKQTLAKY
                     GTLKETASGFRLVLPENIWAGARNSTLASAASAKLEPLAALLASNPDYQILIEAYTDS
                     KGDEVSLQQLTQERARVLSDRFQTAGVDPAKIQANGMGASNPVAPNTTVAGRTKNRRT
                     EITFTAALARSTATN"
     gene            3266..5335
                     /locus_tag="VFH91_04590"
     CDS             3266..5335
                     /locus_tag="VFH91_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862296.1"
                     /translation="MFPLDPLEILQVVGYSVGALLPLWLAVQLVSHRKKLSSLERLLA
                     LLAVTMGGWHTSNLLITLHGLFDLGLNRWTTLLRIADTVAVVSITFAYSFLLHVHLHL
                     WANAHDRPLNRSERIRVYLCYLPTVFLVIAIPRLWSGEYELMLTKLGMFVIPFSFWIA
                     YVLGLIAITELMIARDTSNPSERKIMKTLAASFVGVGVFIFAALGFGLGEGTVVGPYF
                     RTLANLGSLLPSALLAYYIYRYRYLELIIEESLIVVTFATVVLTVYVFGIRTIGDWLT
                     QRYGVRPGLVEALLILALALFAAPLRGWLARRFHRLFEREAVLYKQIVSRIGSYRGHY
                     KDISELLLFVEERATDALGIRQLKIFVDGEIGPVSTNGNGLAPDSWVGLVLERSRQSG
                     WLPVEEDDLLSEKGFTLAYPMRREEKVAGLLAVDAGPSGMTYDARSIIEILADQVAIA
                     IDDWRLLDENLRLERELAERERLAALGRMAATVAHEVKNPLSAIKSIAQVMREDEKLS
                     TDYARDLTLIVGETDRLNQSVTQLLSFARRETPSEQPISADELFRSLVDLFRVNAREQ
                     GTVLSYELKVDTQLTGKSVSALRDALSNLLLNALQATSRGGQVTLSALTVDDHLVINV
                     QDSGSGIPESLRDRIWEPFFTTRQRGTGLGLAIVRKRLQEVGGTAVLLPLQDSAGASF
                     QLRLPLT"
     gene            5511..6152
                     /locus_tag="VFH91_04595"
     CDS             5511..6152
                     /locus_tag="VFH91_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862297.1"
                     /translation="MNPRRFLKPVTLAAFLVLIAISGVNVCAQTTTAQPDNKDSEVNL
                     DTQMYLIVGTNQQVEDPRLPSSLDSVIKQLRVTLPFKNYRLAATLINRVQNESRFELS
                     WIGGPLANSTANMPSTPSFSQFKVRNVKLVRNSEGQQMVQMNGFNFGARIPIQTGAAV
                     ASNAVAPVFNYENTGLQTDISMREGEPVIVGTLNVGPSGDAIILVVSAKRTQR"
     gene            6396..7832
                     /locus_tag="VFH91_04600"
     CDS             6396..7832
                     /locus_tag="VFH91_04600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862298.1"
                     /translation="MIVDDEEASRYGMRRALTTFGYNISEAGSAEAARALLKQQEADL
                     LLLDVNLPGMSGIEFLKEIKAANGNGPLVIIVTAHGSERMAVEAVKAGAHDYLSKPFE
                     LDDLRLVVKNAAETVQLRRENFSLRRRIEVERNQRGSLIGNSEAMQRVRAMIDKVSET
                     DATVLVRGESGTGKELVAREIHERNSARRNASFVAVNCAALPSELIESELFGHEKGAF
                     TGAAARREGKFEQADKGTLFLDEIGDMSSNVQAKLLRALEERRIERLGGNESIPVDVR
                     IVSATHRPLEQEITNGNFRADLFYRLRVVTVDIAPLRDRREDIPLLAETFVRLAADRY
                     ELPQRALGQGTLKRLVEYNWPGNVRELRNTIDRAVIMAEGDEIRVSDLPDEITAGMRS
                     SIPSESDTSGENDGVRVPFTADFREDRREFERRYITRCLEYTQGNVTRAAEILDMHRQ
                     SLQHKLRQLGLGRRYVSVAGAPPENSDS"
     gene            7934..8587
                     /locus_tag="VFH91_04605"
     CDS             7934..8587
                     /locus_tag="VFH91_04605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET6862299.1"
                     /translation="MKARLDAIIQREQADYLDNLLTNNDPLLAEMEKFAAEHRVPIAD
                     REVARFLEITARTTGARKALEIGMAIGYSVVHLARGMGERGLVVTIEPNEEMISAANG
                     YLDRANLMDQVKIERGKALDVMPGIEDTFDLLYIDAVKEEYKQYLDLGIPMLRPGGVI
                     IVDNLLWGGKVAVENAANNNDASTVALREFNSYFVNHPRLLAEVLPIGDGLGYAAKQ"
     gene            8865..>10424
                     /locus_tag="VFH91_04610"
     CDS             8865..>10424
                     /locus_tag="VFH91_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6862300.1"
                     /translation="MISCADTSRNETFFGKTVPPERNILRYVTGDEPESLDPAVSTGQ
                     PEARIYMALYDGLVEYDGKTLAPRPALAETWEVNDDSSELTFHLRHDGRWSNGDPIDA
                     NDFVYSFRRAVSKELASRNAYLAYYIKNAQAYNSAMVFVRDQQTGKFLLAKDFSDGDS
                     NEPLSVKPITQGANEYSAKAQKSSPAGDSAFHQEMHSPVRLSLPGDEKKRNDRLNKDP
                     KLKSAVAGKELVPVRAEDIGVEAVDNYTLRISLVQPAPFFTGLLAHQVFRLVNRKTVE
                     QYGNRWTEVGHIVTCGPFKVKTWKPYDTLVLERDPMYWDAATVKLDEIHFYPMSDNPS
                     IMNLYKVGEVDAVLNHTVPNAWLETVRAKKDYMDASEAAIDFLQINVTRPPMNDVRVR
                     KAFNFAIDKEAWAKWRRIVKPLTAFTPEGIFQNYPQPKGDQFNPERGRQLLAEAGYPV
                     TKNSDGSFSCPKFPVDKVEYIYNTQESNKAMAEWMQAQWKQNLGITISLRNMEWKTFL
                     ETRSKLDYSGFS"
BASE COUNT         2552 a         2549 c         2825 g         2498 t
ORIGIN      
        1 gaggtattcg gcaacgccct tgcccagggt gaagacgatc agcaaaaccg agatcatctt
       61 taacgcgccg aagggtgctt ctggaatcag tcgttgtaat ccaaatatcg ttgaagttcg
      121 gtgaccaccg tcacgtgcca atgcctgctc aaagatcgga acaatcaagc cgccaatcgc
      181 ggtgtcaaga attccgacca tgaccatcga gatcgaggcg agcgtgaaaa ttgcccaatg
      241 aggtcttaaa tattttagca ggcgacgaag atcacgcatc tctggttgcg aagggctctg
      301 ttcagttgct ataaggaagg gtagtagtcg gcccaaaatc taaggagaaa atatacttac
      361 caccacttaa ttgcgtcaag gctgacacat ttccccttcc tcctgtttcg catgatagta
      421 tgcactggac tttccgggtg actgttttgt ggtgtgagtt ataatttttt cgaaagatcg
      481 ggtattagct tttagatccc tttccccggc aggatcacga tttggcacag caggcacaac
      541 taaacagtaa gtacgcgggc aaacgccttc tcattgcgga cgatgatacg gatatgcgtc
      601 tgcttctggc ggagtatttt cgccgtctcg gctttcaggt cgaagagaag gagagtggat
      661 tggcagccct ggaatcggcg atggccaacc gattcgactg ctttatcttc gacgtctcca
      721 tgccgggcat gtctgggctg gagttactaa agcgcgtgcg cgatcgtggc gtgcagactc
      781 caacactctt tttaacggct cacgacgccc ttgccgacaa ggtggcaggc ttcgaagccg
      841 gggccgatga ttatctcgcc aagcctttca gtccccgaga gcttgagtac agagttgaag
      901 ctttgctgcg gcgaggtgga gttgtcacaa ccgacaccac tgacgaggaa cgcatcgagg
      961 ttggggactt aatcatagat aagcgccgtc atgaggttct gcgggaaggc gcaagaatcg
     1021 atctaacccc acttgaattt cagattcttg agttacttgc ttccgagccc ggtcgagcat
     1081 ggtcgagaaa tgcccttcta gatcgagttt ggagtactga ctacgaaggt tatcagcgta
     1141 atattgatcc tcatatcaat cgactcagga agaaacttga gactgatccc aagaatcctc
     1201 gttatgtact gactgtccga ggagtcgggt acaaattaaa cgaggcgccc tgactattaa
     1261 atcgacttga cgagtcgaag acgtttttaa gatgctttgg tggcttagct caggattaat
     1321 cgcgctatgg tttgtgctgg cgtttttggt ccataaaaga ggctgggtcc acatgttgtt
     1381 gctcgctggt ttctccctcc tggttattca gatcgctgcc taccgtaaaa ccaaatacca
     1441 acgctccctg tctggtaagt tgtaacgtcc acttgacctg cttaaatcct tcggcaacaa
     1501 tgccttcgtc tgttgcatca aggcattaag cgttgttctg aaaagcctcc ccaagctgtt
     1561 gtaataatga aattgaggaa tatcaggagt aggtttgtta tgagtttaac gctgtctcat
     1621 cgaaaactcg ctctggtagc agtcatctgt ctactgtgtt cggctttcgt attcgcccaa
     1681 agtgatgtcg aaagaaagac cgtcgcgatc acataccctc tcgatgaaac cgtgactgta
     1741 aagtttcgag gcacgactct gcttccacgc ctaaaaggcg aggcaaaggt caaacgtgcc
     1801 ggtcggcgtg gcacgagggt cgaactgaag atcgacgacc ttcccagggc ttctgaactt
     1861 ggcggcattt acaccacata tgtcctgtgg gccatttcgc ctgatggaca ggtcgataac
     1921 ttaggcgaga tcaaacgcag tgggagttct tttgtagact cgaaattgga tgtgacaacg
     1981 ccgctgcaaa cctttgcatt gattttgacc gcggaacctc attttttgat gaaggtaccc
     2041 agccgcatgg tggttatgga aaacttacca ccgaccaggc ctaacggaag tcagatcgca
     2101 acggctgacg taaggtacat cggaaattcg agtgattact tccgtgatgc gcgtgttccc
     2161 ggaattgcag acgccgatta caaacagacg ccggtgtcgt tgttgggcgc tcgacaagca
     2221 gttaacctgg cgaaatacgc gggtgcttct caggatgcac cagatgaact gcgaattgcc
     2281 gaagaacaac tacagtcagc cgagaagaag tggcgtctga atcaacccgt cgtggaaatt
     2341 gatatggaag cccgttccgc tacttcagct ggtgctcatg ccgaagaagt ggcctacgct
     2401 cgaaaagcag cacgactgca acgtgacgag ataaggcgaa gagatgaagc ggtacgctcc
     2461 gccgaaaaaa ctgctgacga atctcagcgc gaggtgtcgc agctaagagc agcgcttgag
     2521 aaggagcaac gagcgcgtga actagcggag cgagatgcag gtacggccaa tgatcaactt
     2581 cgcgaaaaac gagttgagat tgcccaactg cgtgatgaat tgcagacact gagatcagaa
     2641 agcgaatcgg caaaacttaa actggctcgt ctggaaggtg agaaacaagc ggaaacagat
     2701 cgcgttgaag ctgaacgacg cgcacaggaa cgccgtgcgg ctgaagcaaa tctgaagcaa
     2761 accctcgcta agtacggaac actgaaggag actgcttccg gctttcgatt ggtgttgccg
     2821 gaaaatattt gggctggtgc tcgaaactca actctagctt ccgccgcgtc cgcaaagctg
     2881 gaacctcttg cagccctgtt ggcgagcaat ccagactacc agatcctgat cgaggcttat
     2941 actgacagca aaggagacga agtctcgttg caacagctaa cccaggaacg cgctcgcgtt
     3001 ttgtctgacc gttttcagac ggcgggagtg gatccggcaa agattcaagc taacggtatg
     3061 ggtgcctcga atccagtcgc accgaataca accgttgccg gaaggaccaa aaatcgtcgg
     3121 actgaaatca cttttactgc ggcgctcgcc cgaagcactg caacaaactg aattgtaggg
     3181 gcgtcactcc gtggacgccc gttgccgaaa cggagcctca ggccggagac aaggggcgcc
     3241 cacggagtga cgcccctacc gttcgatgtt cccacttgat ccactcgaaa tcctccaagt
     3301 tgttggttac agcgtcggag ctttgttgcc tctgtggctc gctgttcaat tagtctcaca
     3361 ccggaaaaaa ctttcctcgc tcgagcgcct gttggcactg ctcgccgtaa cgatgggcgg
     3421 ctggcacact agcaatctgc tcattactct tcatggtctt tttgatcttg gcctcaatcg
     3481 ctggaccact cttttaagaa tcgctgatac ggtagctgtt gtaagtatca cgttcgctta
     3541 ctcgtttttg ctccacgttc atctccatct ttgggccaat gctcatgatc gtcccctgaa
     3601 tcgcagtgaa cgaattcgcg tctacctttg ttacctgccc accgtctttc tggtgatagc
     3661 cattccgcgt ctatggtcgg gtgagtacga gctcatgctc accaagctgg gtatgtttgt
     3721 cattccgttc tcgttttgga ttgcgtacgt acttgggctc attgcaataa ccgagctgat
     3781 gatcgctcgc gatacgtcga atcccagcga acgcaagata atgaagactc tggccgcatc
     3841 gtttgtcggt gtcggcgtct ttattttcgc ggcccttggg tttggtcttg gcgaaggaac
     3901 agtagttggt ccttacttca gaacgctcgc gaacctcgga tctttactac cttcggcact
     3961 cctcgcttat tacatatatc gctatcgtta tttggaactg atcatcgaag aaagtctgat
     4021 tgtggtgacg tttgccacag tggtgctgac ggtctacgta ttcggcatcc gtacgatcgg
     4081 cgattggttg actcagcggt acggtgtcag accaggtctg gtcgaggcgc tgctaattct
     4141 ggccttagct ctctttgctg caccgttgcg tggttggttg gccagacgtt ttcatcgact
     4201 gttcgagcgc gaagcggtcc tctacaagca gatcgtgtcg aggatcggtt catatcgtgg
     4261 ccattacaag gatatctcag agcttttact gttcgtggaa gaacgagcaa ctgacgcgct
     4321 cggcatacgt cagctcaaaa tattcgttga cggtgaaatt ggaccagttt cgactaacgg
     4381 aaacggtctg gcgccagatt cctgggtggg cctggtgctg gaaagatctc gacagagtgg
     4441 atggctaccg gtcgaagagg acgacctctt atctgagaag gggttcacac ttgcgtatcc
     4501 gatgcgacgc gaagagaagg tcgcaggact cctggctgtc gacgcggggc caagcggaat
     4561 gacttacgat gcgcgttcca tcatcgagat acttgctgac caggtagcga ttgcaatcga
     4621 cgactggcgc ctcctggacg aaaacctgag attggaacgc gaacttgctg aacgcgagcg
     4681 tctcgcggcc ctgggtcgta tggctgcaac ggttgctcac gaagttaaga acccgttgtc
     4741 ggcaatcaag tccattgctc aggttatgcg tgaagacgag aagctgagta ctgattatgc
     4801 tcgcgatctg actttgatag ttggagaaac cgatcgcctg aaccaaagtg tcactcaact
     4861 actgagcttt gctcgaaggg aaacaccgtc tgagcaacca atcagtgccg acgaactttt
     4921 ccgttcgctc gttgatctct ttcgcgttaa tgcacgcgaa cagggaacag tgctcagtta
     4981 tgaactaaag gttgataccc aactgacagg aaagtcagta tcggctctgc gcgatgcact
     5041 ctcgaacctt ctattaaatg cgctccaggc tacgtcccgc ggtggacaag tcacactttc
     5101 agcgttaact gtcgacgatc atcttgttat taacgttcaa gattcggggt caggaattcc
     5161 ggagagtctg cgtgatcgca tctgggaacc cttcttcacg actcgtcaac gtggcaccgg
     5221 tcttggtctg gctatcgtga ggaaacggtt acaggaagta ggcggcactg ccgttcttct
     5281 gccgctccaa gatagtgcag gcgcctcttt tcagctgcgt ctgccgctta cttgatcgac
     5341 ttcggtcgac gtcctcctgg gttgactgaa agtcggcaac ccgtaatcgg caagatccgg
     5401 gaactttgtt gccagctaag cgtgtgtgct gccagttaga ttttgcgact ccacacagaa
     5461 tggtgttggt ccaatccgca tccgtaagtt ttgaaggaga gctaagacag atgaacccca
     5521 gaagatttct aaagcccgtc actcttgcag cgtttcttgt tttaattgct attagtggcg
     5581 tcaacgtctg cgcccaaaca actactgccc agccggataa caaagacagc gaagtcaatc
     5641 tcgatacaca gatgtatttg attgtcggaa ccaatcaaca agtcgaggat ccacgtttac
     5701 cgtcctcact cgattcagtg atcaagcagt tgagagttac tttgccgttt aagaactatc
     5761 gacttgccgc gacgcttata aaccgggtgc aaaatgaaag tagatttgag ctgagctgga
     5821 tcggggggcc gctggctaat tcgacggcta atatgcccag cactccgagc tttagccagt
     5881 ttaaagttcg gaacgtaaaa ctcgtgcgca acagtgaagg ccagcagatg gtgcaaatga
     5941 atggattcaa ctttggtgcg aggataccga ttcagactgg cgcggctgtg gcgtcaaatg
     6001 cggttgctcc ggtttttaat tacgagaaca cggggttgca gaccgacatc agcatgagag
     6061 agggtgagcc agttattgtt ggtacgctga atgttggacc atcgggtgac gcgattattt
     6121 tggtggtatc ggcgaagcgg acgcagagat agagctgttg agtaggagtt tggattaaca
     6181 aagcgccgcc gggccggcgc actccaaaag ggcgggcaaa ttgctcgtcc ttttttgtta
     6241 tgcggagaag gagggcgggt tcgtaggtga gatttggatt aacaaagcgc cgccgggccg
     6301 gcgcactcca aaaagtgtgg cacgagacgc gctaggggat ttgtggttca aggtcttaaa
     6361 cttacccgga agtgagaaaa gatactttgc gattaatgat tgttgatgac gaagaggcct
     6421 cgcgctacgg gatgcgccgc gccctcacga cctttggtta caacatctca gaagctggtt
     6481 ccgccgaagc cgcccgcgcg ctcctcaaac aacaggaagc cgacctgctg ttgcttgatg
     6541 tgaacctgcc gggaatgagt ggcatcgagt ttctgaaaga gatcaaagcc gcaaacggca
     6601 atggtccact cgtcatcatt gttacggccc atggttcaga gcgcatggct gtcgaagcgg
     6661 tcaaagcggg cgcccacgat tacctgtcga aacctttcga actcgatgac ctgcgattgg
     6721 tggtaaagaa tgctgccgaa actgtccaac ttcgacgcga aaacttttcg ctgcgccgtc
     6781 ggatcgaagt tgagcggaat caaagaggtt cgctcattgg aaattccgag gcgatgcagc
     6841 gtgtccgagc gatgatcgac aaggtgtcgg aaacagacgc gaccgtgctc gttcgcggag
     6901 aatcgggtac cggcaaggaa cttgtggctc gtgagattca cgagcgaaat agtgcgcgcc
     6961 gcaacgcttc atttgtggcc gtgaattgtg ctgcgctgcc ttcggagttg atcgaatcgg
     7021 agctctttgg tcacgaaaaa ggtgccttta ccggcgcagc agcacgacgg gaagggaagt
     7081 ttgaacaggc tgataaagga actctttttc tggacgaaat cggcgatatg agctcaaacg
     7141 tccaggcgaa gctcctccgt gcgttagagg aacggcgcat cgaaaggctt ggcggcaatg
     7201 agtccatccc ggttgatgtt cgcatcgtga gtgcgactca tcgtccatta gagcaagaga
     7261 taacgaacgg caactttaga gcagatctgt tttaccgctt gcgagtcgtt acagttgata
     7321 tcgccccgtt gcgtgatagg cgcgaagaca tccccttgct tgctgaaaca ttcgtgcgtc
     7381 tggcagcgga ccgctacgag ttaccccaac gagcccttgg ccagggaacg ctaaaacggc
     7441 tcgttgaata caattggcca ggaaatgtgc gagagttaag aaatacgatc gaccgtgctg
     7501 ttattatggc tgaaggcgac gagatcagag ttagcgattt gcctgacgag attaccgctg
     7561 gaatgcgtag ttcgatacct tccgaaagtg atacctcagg tgaaaacgat ggagttcgag
     7621 ttccatttac tgccgacttc cgcgaggatc gacgtgagtt tgaacgacga tatatcacac
     7681 gatgtcttga gtatacgcag ggaaacgtga ctcgagcagc cgagattctc gacatgcatc
     7741 ggcagtcact tcagcataag ctacgtcagc tcggactcgg ccgccggtat gtttcggtcg
     7801 cgggcgcacc gccagaaaat tccgacagtt gacgttgggt ggcactggcg gccgccccca
     7861 atttagtctt aggaagttat gatcatgagt gggcggccca gcagtgccgc ccaatcactt
     7921 ttccggagga acaatgaaag caaggttgga cgccattatt caacgcgaac aagcggatta
     7981 cctcgataat ctactaacaa acaacgaccc gttactggct gagatggaga agtttgctgc
     8041 tgagcatcgt gtcccgatcg cggatcgcga ggttgcaaga tttctcgaga tcaccgctcg
     8101 tacaactgga gcccgcaaag cactcgaaat tggaatggcg ataggatact cagtggtcca
     8161 tcttgctcgg ggaatgggag aacgtgggtt ggttgtcacg atcgagccaa atgaggagat
     8221 gatcagcgcc gccaatggtt atctggatcg agcaaacttg atggatcagg tgaagattga
     8281 acggggcaaa gcgctcgacg ttatgccggg catcgaagac acattcgatc ttctgtatat
     8341 cgatgctgtg aaagaagagt acaagcaata tctggatctt ggaatcccga tgttgagacc
     8401 cgggggagtg atcatcgtcg acaatctttt atggggtggt aaagttgcag tcgaaaatgc
     8461 cgccaataat aatgacgcct caacggtcgc actgcgagag ttcaattctt actttgtgaa
     8521 tcatcctcga ctcctggctg aagttctgcc aattggtgat ggattgggat acgcggctaa
     8581 gcagtgatgg aagttgggcg gggcctgatt ctgcaaagca cggaacaatt tgttgcgctg
     8641 cgctttcctt gcgtgctaat tttctcgcgt ctttcgatgt tttcgtggag actctctcca
     8701 aaaattgcct cacaaggcga gttctcaaac tcgctgcttt aacgcttcgg tgatcaaacc
     8761 gaacgatcgg cccagatccc cgggcattga gtggagtgac cttgatgagc atcaattgta
     8821 agttcctggt gacaatttgc gtgtttgtgg caacggcgtg cacaatgatt tcgtgcgcag
     8881 atacctcacg taatgaaacc ttttttggca agacggtccc tccggaacgc aacatcctgc
     8941 gttacgttac tggtgacgaa ccggagtctc tcgatcccgc tgttagtacc ggtcaaccag
     9001 aagctcgtat ttacatggca ctctacgacg gactggttga atacgatggc aagacgctcg
     9061 ctcccaggcc tgctctagca gagacgtggg aagtgaatga cgactcgtct gagcttactt
     9121 tccacttacg ccacgacggc cgctggtcca acggcgatcc tattgacgca aacgattttg
     9181 tttacagttt caggcgtgct gtgtccaaag aactagcttc tcgcaacgcc tatcttgctt
     9241 actacattaa gaacgcccag gcttataact cggcaatggt gtttgttcgt gatcagcaaa
     9301 cagggaagtt tcttttggca aaggactttt ctgacggcga ttccaacgaa ccacttagcg
     9361 tgaaaccgat tacgcaggga gcaaacgaat attcagcaaa ggcacaaaaa tccagtcctg
     9421 caggggattc tgcatttcat caggagatgc attctccggt gcgcctttcg ttaccgggtg
     9481 acgaaaagaa gcgcaatgat cggctaaata aggatccgaa attgaaaagc gcagtggcgg
     9541 gaaaagagtt ggttcctgtc agagcggaag acattggtgt cgaggccgtc gataactaca
     9601 ctctcagaat ctctttggtc caacccgctc ctttctttac cggcctcttg gcccatcagg
     9661 tttttcgcct ggttaacaga aaaactgtcg agcaatacgg caatcgttgg acagaggtgg
     9721 gacacatcgt cacgtgtggt ccattcaagg taaagacctg gaaaccttac gacaccctgg
     9781 tgcttgaacg agacccgatg tattgggacg cagccacggt taagcttgac gagattcact
     9841 tttatccgat gtcagacaat ccttcgatca tgaaccttta caaagtcggc gaagtggatg
     9901 ctgtactgaa tcacacggtg ccgaatgcgt ggctcgagac ggtgcgcgcc aaaaaggact
     9961 acatggacgc ctcagaagcc gcaatcgatt ttcttcagat caatgtaacc aggcctccga
    10021 tgaacgacgt gagagtgcga aaggctttca actttgcgat cgacaaggaa gcctgggcca
    10081 agtggcgcag gatcgtcaaa ccattgaccg catttactcc cgaaggcatt tttcagaact
    10141 atccgcagcc gaaaggtgat cagtttaatc ctgaacgggg gcggcaatta ctcgctgagg
    10201 caggatatcc cgtcacgaaa aattcggatg gaagtttctc ttgtccgaag tttccagtcg
    10261 ataaggttga gtacatctac aacactcagg aatcaaacaa ggcgatggcc gaatggatgc
    10321 aagcccagtg gaagcaaaac cttggcataa ccatcagcct gcgcaatatg gagtggaaaa
    10381 catttttgga gacgagatcc aaacttgatt atagtggctt ttct
//
LOCUS       DASSFP010000212         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1092335, whole genome shotgun sequence.
ACCESSION   DASSFP010000212 DASSFP010000000
VERSION     DASSFP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092335"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..593)
                     /locus_tag="VFH91_04615"
     CDS             complement(426..593)
                     /locus_tag="VFH91_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862301.1"
                     /translation="MKQLFVPARLLQVRESTFIFEIAIHDRGPSRLPVRDIETGPAIR
                     QGRLGLTYFSD"
     gene            605..2011
                     /locus_tag="VFH91_04620"
     CDS             605..2011
                     /locus_tag="VFH91_04620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862302.1"
                     /translation="MYSERVLLIHNQSDTKVFPILTDILDPHFKVDRATYSSTTRHND
                     VGEFVRHLDKTIKQNNPDLVVLIASDGSLSHRAHVLKSINETAGEIPVMLALEPCQSD
                     EVIELLQLGASDFIVSPFTAQEVLTRAWRLLRIFEPQAKSVSNGARPGFNKIIGESAA
                     FLVQLRRIPIIAKCEANVLIGGETGTGKELYARAIHYSSPRAGKPFMPVNCGAIPIEL
                     VENELFGHQRGAFTSASTLQPGLIEEANGGTLFLDEIDCLPLSAQVKLLRFLQEKEYR
                     PLGSSKVRRADVRIIAASNLNLEEAVGNGKVRQDLFYRLNIISLLLPPLRDRREDIGL
                     LAGHFLKKYSAEFDKDVEALSDEALNVLMVHNWPGNVRELEHVIERAIVMCEGSTLQV
                     GDIILSSTDTQAPQQSLQEAKAQEIARFERNYIQGLLSACKGNITRAAQVARKNRRAF
                     WQLIQKYEIDVAKFKTAR"
BASE COUNT          587 a          536 c          550 g          522 t
ORIGIN      
        1 ctccactagc atccgcctgt tacaaatagt taagacgagt taaacacctc gtctcctacg
       61 ttcaaaggac cccgcccaga agtccccgga cgcagtgctt gttccagtta cggccatgcg
      121 tcatcaactt atttaccagg cttcaaaacg cacgttcgcg tccaattagc cgttcagcga
      181 cgcatctgaa ccgccaaatt tcagacgcat tttcaagccg ccgacagaag atagcggttc
      241 catataaatg gagcgtgaac gggcggaggg aatatttaca aatgaaaaac caatgctcgt
      301 ctgtggcgtt ttctaaacac ctgccctgct cgatacttcg caatcgagct agcatctgcc
      361 ctgttgattc agccttcctc tgtaatcaca ggagatcgtt ctcaatagga gtcgaagtct
      421 cacgcttaat ctgagaagta agtaagtccc agtcgaccct gccggatcgc cggcccagtc
      481 tcgatgtcac gcaccggcaa gcgagatgga ccacgatcgt ggatcgctat ctcgaaaata
      541 aaggtgcttt cccgaacttg aagaagcctc gctggcacaa acagttgttt caagggaaag
      601 agtcatgtat tccgaacgag tcctcctcat acacaatcag tcggacacca aagtgttccc
      661 aattctcacg gatattctgg acccccattt caaagttgat cgagcgacct attcgagtac
      721 gaccagacat aatgatgttg gagaatttgt caggcatctc gataagacaa ttaaacaaaa
      781 caatccggat cttgttgtgc ttatcgcttc ggatggttcg ttgtcgcatc gagcgcacgt
      841 gttgaaatcg atcaacgaaa cagcgggcga gataccagta atgcttgccc tcgagccatg
      901 ccagtcggac gaagttatcg aacttctgca attaggtgca tccgacttta tcgtgtcgcc
      961 gttcacggcc caggaggttc tcacgcgagc ctggagattg ctgagaatat ttgagccgca
     1021 agcgaagtct gtttcaaacg gggctcggcc cggattcaat aagataattg gagagagtgc
     1081 ggcgtttttg gtccaacttc gacgcattcc aattattgca aaatgcgaag ccaacgtgtt
     1141 gattggtggt gaaacaggaa ccggcaaaga actctatgct cgcgcaattc actacagtag
     1201 tccgagggcc ggaaaacctt tcatgccggt caactgtggc gcaattccga tcgagctggt
     1261 tgagaatgag ttgtttggac accagcgagg cgcatttaca agcgcctcga cgcttcagcc
     1321 tggtttaatt gaggaggcaa atggtggcac actttttctc gacgagattg actgtttacc
     1381 cctttcagct caagtgaagt tgctgagatt cctgcaggaa aaagaatatc gaccgcttgg
     1441 atcgtccaaa gttcgacgcg cagatgttcg aatcattgca gccagtaact taaacctgga
     1501 ggaagcagtc gggaacggca aagtgcgcca ggatctattt taccgtttga atattatttc
     1561 acttttgttg ccgccacttc gggatcgccg cgaggatatt ggattgcttg ccggccactt
     1621 tttgaaaaag tattcagccg aatttgataa agatgttgag gccttatcag atgaagctct
     1681 gaatgtgttg atggtccaca attggcccgg gaatgtaaga gaacttgagc acgtaatcga
     1741 gcgtgctatt gtgatgtgcg agggatcaac gcttcaggtt ggcgatatca ttttgtcgtc
     1801 aaccgatact caggcgccac agcagtcgtt gcaagaagcg aaggcacagg agatcgcccg
     1861 gtttgagaga aactatattc aaggtctttt gtcggcatgc aagggaaaca tcacgcgagc
     1921 agctcaagtc gcgcgtaaga acaggcgggc gttctggcaa ctgatccaga aatacgagat
     1981 cgatgtcgcc aaattcaaga ccgctcgcta agcgcagcag agccgcaacc aaagaaagat
     2041 gtggctggaa cagtaccgat tccggagatg tccgatatgc ttcatcagct tgtcggtcat
     2101 gatgcacact caagttcaga ggaacgaatc aacggatgac cgacaagctg aagcatatcg
     2161 gacattccgc tagaccctgg gaaattgaat tctaa
//
LOCUS       DASSFP010000213         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_355686, whole genome shotgun sequence.
ACCESSION   DASSFP010000213 DASSFP010000000
VERSION     DASSFP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355686"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFH91_04625"
     CDS             <1..227
                     /locus_tag="VFH91_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862303.1"
                     /translation="PASYANVDGSLLDKLQQRLQQIIERGGEAFLTTTVLHGRRALRV
                     NINSFLTEQRHVDDLLELLQRCGREAIRES"
     gene            246..2141
                     /locus_tag="VFH91_04630"
     CDS             246..2141
                     /locus_tag="VFH91_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6862304.1"
                     /translation="MISRCFLVLLVLVFAGSAAYAQAQTGPKSLPAIRSQVQFDSISI
                     TYDPNTPYALPHILFVIDRKNGNKIYYVNKKRYSFHKDFVNGTYLSLERGKEFFENNY
                     LKENRRFILGTVAYQTPIKRWSFEFWEGDLIPPDLIQLTYEIINKTFFTPVAFKPNSL
                     RQDEVSSSLPAVKRILQSDISKEQEYQALNMAKGLGRIHIISKLDDHVEIGFNEILVL
                     DEVPIQLPPVAGIITAQPSTPLSHINLLAKGWGIPNAYIKNAKELFKQYDGWWVSFET
                     LRDKYTIKRADLNQLKEYQRRLSERLDVMKPRSDLSETRLLNLSQQRSNLSIAFGGKS
                     ANLGEVMNAKLPGIVVPGGFAIPFYYYDAFIKRNNLDDAIYALLNDQKFVHDPAYRRE
                     QLVKLRDQIQRAEFDPDLRKDVLDKVARDYRGKGLFVRSSSNSEDLPNFSGAGLYTTV
                     PNVRGDDELINAIKTVWASLWNFEAYEARERANVDHSKIYMAVLIQEGINSESSGVMI
                     STDPFDTENKGAIYISAKRGLGIKVVEGQRIAEQIIFRPRTNAVQVLTRSAEDSLLTF
                     DDKGGVKEIPITGDRVVLTDEVIRRLVRAAVEIKRVFASRDQDIEWAYMKGQIYIVQS
                     RPFIPGS"
     gene            2512..3621
                     /gene="hisZ"
                     /locus_tag="VFH91_04635"
     CDS             2512..3621
                     /gene="hisZ"
                     /locus_tag="VFH91_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET6862305.1"
                     /translation="MTVSKIPSGMRYYIGPEARLRRWIEDETMAVFQGWSYEEIITPS
                     VDYYSLFEQGMGQSESRTAFRFTDNDGHLLSLRPDVTSSIARVAATMLARAPRPLRFC
                     YASPVFRQQLQSRAEWRRENTQLGCELIGCNGNTSDLEILLVAAEILARVRLTDHSII
                     TLSHAGIFDGLFSESGLDQDARMEIQGLLNSMDQTGLKSFFDRNQISKTVAESILLLL
                     HLSGKEEVLVHARESITNSGSVAALDSLQKIWTVIQSLGLENRFSLDLADVSGLQYYT
                     GMIFKIYIAGAGYRVGRGGRYDGLTSKFGAAEPAVGFVLSLDALTEVLSRHQLNISTD
                     STQGSVVQREDLKSTFAEALTRRARQDQIRIEVPE"
     gene            3618..4253
                     /gene="hisG"
                     /locus_tag="VFH91_04640"
     CDS             3618..4253
                     /gene="hisG"
                     /locus_tag="VFH91_04640"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET6862306.1"
                     /translation="MITVAIAKGRMQDDAIALLAKAGVHLHSAALASRRLAVEDSSGD
                     YRFIFVKPMDVPVYVEHGIADCGIVGRDVILETQADVLIPLDLGIARCQLVVAAIDKD
                     VLTRSLGMLRIATKYTHLAASHFGALGIPVEIISLSGSVELAPVLGLADAIVDLAETG
                     RTLKENGLNVVDVISESTGRFVVNRASYQLKAETMSKFTDSIERALEMNSK"
BASE COUNT         1101 a         1034 c         1128 g         1105 t
ORIGIN      
        1 ttcctgcctc atacgcaaat gtggacgggt cgctgctgga taagttgcag caacggttgc
       61 aacagatcat cgagcgaggc ggtgaagcgt ttctgacgac gacagttttg cacggcagac
      121 gtgcgttgcg agttaatata aacagctttc taaccgagca gcgtcatgtg gacgatttgt
      181 tagaattact tcagcgttgt ggtcgtgaag caatcaggga gtcatgaaat tcttcaagta
      241 tgcaaatgat tagtcgatgc tttctggtgc tgttggtgct ggtcttcgcg ggcagtgctg
      301 cttatgctca ggctcagact gggccgaaat ctttaccggc tattcgctct caggttcaat
      361 tcgattccat cagtatcact tacgatccga acactcccta cgctttgcct cacatcctgt
      421 tcgttatcga tcggaaaaat ggaaataaga tctactacgt caataagaag cgctacagct
      481 ttcacaaaga ttttgtaaac ggtacatatc tatctctgga gcgcggaaaa gagttttttg
      541 aaaacaacta tctaaaagag aatcgtcgtt ttattcttgg gacggttgcg tatcaaacgc
      601 caataaagcg ctggtccttc gagttttggg aaggcgatct tattccgccg gatcttattc
      661 agctgacata cgaaatcatc aacaagacct ttttcacccc ggtcgctttc aagccaaact
      721 ctttacggca ggacgaggta tcgagttcgc tgccagccgt gaaaagaata ttgcaaagtg
      781 acatttcgaa agaacaggaa tatcaagcgc taaacatggc taagggtttg ggaagaattc
      841 atattatttc gaaactcgat gatcatgtcg aaattgggtt caacgaaatc ctggttcttg
      901 atgaagtacc gattcagttg ccgccggttg ccggcatcat cactgctcag ccttcgactc
      961 cgttgtcaca cattaatttg ttggcgaaag gctggggcat tcccaatgca tacatcaaga
     1021 atgccaagga gctttttaag cagtacgacg gttggtgggt ctcatttgaa actcttagag
     1081 acaagtacac gatcaaaagg gccgacttaa atcaattaaa agaatatcaa cggaggcttt
     1141 ccgagcggct ggatgttatg aagccgcggt cggacctgag cgaaactcgt cttctgaatc
     1201 tctctcaaca acgctcgaac ttaagcattg catttggcgg caagtcggca aacctcggtg
     1261 aagttatgaa tgcgaaattg ccggggatcg tcgtgccggg tggttttgcc atcccgttct
     1321 attactacga cgcgttcatc aagagaaata atctcgacga tgccatctat gcccttctaa
     1381 atgatcagaa gttcgttcac gatcctgcgt accgccgcga acaattggtc aaactacggg
     1441 accaaattca acgtgccgaa tttgatccgg atctgcgcaa agatgtgctg gataaagtcg
     1501 cgcgagacta ccggggcaag ggattatttg ttcgcagctc ctcgaactcc gaagatttac
     1561 cgaattttag tggtgcaggg ctatacacga ctgttccaaa cgttcgcggt gacgatgagc
     1621 tcatcaacgc aatcaagaca gtttgggctt cgttatggaa cttcgaagca tacgaagcgc
     1681 gcgaacgggc caacgttgat cattcgaaga tctacatggc cgtcctgatt caggaaggca
     1741 tcaactctga gagttctgga gtgatgatca gcactgatcc atttgacact gaaaataaag
     1801 gcgccattta catcagtgcg aaacgtggcc tgggaattaa agtggtcgag ggccagagaa
     1861 ttgccgagca gatcatcttt cggcccagga caaacgcggt ccaggtgcta acccgttcag
     1921 ctgaggacag tctgttgacg ttcgatgaca agggtggcgt caaagagata ccgataaccg
     1981 gcgaccgcgt cgttttaacc gatgaagtca tacgccgctt agtgagggca gccgtggaga
     2041 tcaagcgagt atttgcaagt cgggatcaag acatagaatg ggcatacatg aaaggacaga
     2101 tctatattgt tcaatcaagg ccctttattc ccggtagcta gttaaacact cgatctcagc
     2161 gtctctctgc gagatctctg cgtctctctg cttttctata tacccggtac tgagttcaac
     2221 gcagagggac gcagagatct cgcagagaga cgcggagata acttcaaagc tttcgactcg
     2281 atttgacaac cttctgcgcc gtcggctata accgcccacg taatgttcgc aagcaatcga
     2341 catgggcacc attcctactg gcatcaccac cactgtggtg gcgggccccg cgattgtgtt
     2401 tgccgcgtcg gaaatgaata gataaagacg cgaaaacgat ttcgaacccg ctgccaaccc
     2461 aaaaggtcgg cggcgggttt ttgcttttag cgtcaataac ttacagacga tatgacagtc
     2521 tccaaaatac cttccgggat gcggtattac atcggtccag aggcccgact ccggcgctgg
     2581 atcgaggatg aaacaatggc agtctttcaa ggctggagtt acgaagagat catcacaccg
     2641 tcagtcgact actactcact ttttgagcaa gggatgggcc agagcgagtc tcggacggcg
     2701 ttccggttta ccgacaacga tggccatctt ctgagtcttc gtcccgatgt gacgtcttcg
     2761 attgcccgcg tcgcggcaac tatgctggct cgagcgccac gaccacttcg cttttgttat
     2821 gcatctccgg tttttcgtca acagcttcag tcgcgtgcag agtggcgtcg tgaaaataca
     2881 caattaggtt gcgagctaat tggttgtaat ggcaatacct cagatctgga gatactgctc
     2941 gtagcagccg agatactcgc tcgggtacga ctcaccgatc actcgatcat tacccttagt
     3001 cacgctggta tctttgatgg actctttagt gaatcgggcc tggaccagga cgctcgcatg
     3061 gaaattcagg gtctcttgaa ttcaatggac cagactggcc taaagagctt ttttgaccgg
     3121 aaccaaatct caaaaacagt ggccgaatcg attctgttat tgctacacct ttcagggaaa
     3181 gaggaagtac ttgtccatgc tcgtgagtca attactaact ccggatcagt ggctgcgtta
     3241 gattcactgc aaaaaatctg gactgtcatc cagtcgctgg gtctggaaaa caggttcagt
     3301 ttagatctcg cggacgtttc gggtctccag tactacaccg gaatgatatt caagatttac
     3361 atagcaggtg cgggctaccg cgttggacgg ggaggccgtt acgacggtct gacctcgaag
     3421 tttggcgcgg ccgagcctgc agtcggtttt gtgctgagtc tggacgcgtt gaccgaagtt
     3481 ctcagtcgtc atcagctaaa catctcgacc gactcgacgc agggcagcgt agtgcagcgt
     3541 gaagatctca agtctacttt cgctgaagct ttaactcgca gggcgaggca ggaccaaatt
     3601 cgaattgagg taccggagtg attaccgtcg ccattgcaaa agggagaatg caagacgatg
     3661 cgattgcgct gctggcaaag gctggagtgc atttgcattc cgccgcgctg gcgtcacgac
     3721 gtttggccgt cgaagattct tcgggtgact acagatttat tttcgtcaaa ccaatggatg
     3781 tgcctgtcta tgtcgaacac ggaatcgctg actgtgggat cgttggtcga gacgtcattc
     3841 tcgaaactca agctgatgtt ttgattcctc tggacctggg tatcgcgcgt tgccagcttg
     3901 tggttgcggc aatcgacaag gatgttttga cgcgctcgtt gggaatgctg cgaattgcga
     3961 caaagtatac gcatttagca gcttcgcact tcggtgctct gggtatacca gttgagatta
     4021 tttcgctctc aggctcggtc gaacttgcac ctgtccttgg tctagccgac gccattgtgg
     4081 accttgcaga aacaggcagg actttaaaag agaatggact gaacgtagtg gatgttattt
     4141 ccgaatctac gggtcgcttc gtagtgaatc gggccagtta ccaattgaag gcggaaacga
     4201 tgtctaagtt tactgattcg atcgaacgag cactggaaat gaatagcaaa tgatccgaat
     4261 tatcaaaaca aaggagcagt tgcagtcttt tatcgcacaa ctccggcagc gtgcgaccgg
     4321 tgcggatccc gacctcatta cttcggtaag tcaagtcatt tcaaatgt
//
LOCUS       DASSFP010000214         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1389178, whole genome shotgun sequence.
ACCESSION   DASSFP010000214 DASSFP010000000
VERSION     DASSFP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389178"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFH91_04645"
     CDS             complement(<1..749)
                     /locus_tag="VFH91_04645"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET6862307.1"
                     /translation="MTQQNQVNTQAETKSVDPLAIHYRAIAVDMHADTVQRVLDEHVD
                     LQKQLVDGHFDAVRARSGGLDAQFFSIWVEPQLFGGGGPRCVKRADDQISAIRTLAEK
                     HPETWQFATSAADIRSAAKQGKIAALLGLEGGYAIDERLENVERYYKMGVRYMSPAWS
                     VSTSWAGSSGDDVGSSRGLNDFGKSVVREMNRLGMMVDVSHVSDKTFWDIVHTSTKPV
                     IATHSACRAIADVPRNLTDEMIQALAKTGGVV"
     gene            1078..2151
                     /locus_tag="VFH91_04650"
     CDS             1078..2151
                     /locus_tag="VFH91_04650"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET6862308.1"
                     /translation="MFSKEGKAKDIRTPKICDVVWHNGEFIKWSDARVHVMSHVLHYG
                     SSIFEGIRCYSTEKGPAIFRLREHMQRLLNSAKIYRMEHEWTLDQLCDGAVELVRRSQ
                     MDQCYIRPILFRSLDEENPAFGVNPFPNPLACYIGAWDWGKYLGDEAIEQGVDVCVST
                     WNRLTPNSMPAMAKSGANYMNSQLIKMEALLNGFAEGIALDDRGYVSEGSGENIFLVA
                     GDVVLTPPLSSSILPGITRDSVIQICQQLGITVKERTIQRAALYVADELFFSGTAAEI
                     TPIRSVDRITVGKGGRGEITEKIQKTFFEITKAKRPAPGDWLTYVDRKRQSDDNDAQS
                     GNIESSGSHDGEPALTLQALSSD"
BASE COUNT          554 a          585 c          585 g          570 t
ORIGIN      
        1 accacgccgc cggtctttgc cagcgcctga atcatttcgt ccgtcagatt tcgtggcaca
       61 tcggctattg ctctgcacgc agaatgagtg gcgattacag gctttgttga ggtgtgcacg
      121 atgtcccaaa aagtcttgtc cgacacatgc gaaacatcga ccatcatgcc cagtcgattc
      181 atttcacgaa cgacactctt tccgaagtcg ttcaaaccac ggctggatcc aacatcgtca
      241 cctgacgaac ccgcccaact tgtgctgaca ctccatgcgg gtgacatata gcgaacgccc
      301 attttgtaat agcgctccac attctcaagg cgctcgtcga tcgcataacc accctccaaa
      361 ccaagcaagg ctgcgatctt accctgcttt gcggcgcttc gaatgtctgc ggcggaagtt
      421 gcgaactgcc aggtttcggg gtgtttttca gctaaggttc tgatcgcaga gatttgatca
      481 tccgctcgct taacgcaccg cggccctcca cccccaaata gctgaggttc aacccaaata
      541 gagaagaact gtgcgtcaag tccacctgaa cgggcccgta ccgcatcgaa atgaccatcg
      601 accagttgct tttgcagatc aacgtgttcg tcgagtacac gctgcaccgt gtcggcgtgc
      661 atgtcgacgg caatagctcg ataatggatg gcgagcggat cgacagattt ggtctccgct
      721 tgagtattaa cttgattctg ttgtgtcatc gttttgaatt ggcacgcgtt cagtgtcagg
      781 aggatcacga ggaccccaaa cgtagcaaac gcgcggtcat ttacggattg taggaaggtc
      841 tcaatgattc gcaatctctt ttggtttagt aaggccgatt ttgatgttac gtaaccatac
      901 ttgcgcgagg caagcccgtc ttgccaatgg aactctaact actgtattgt gccgagtctg
      961 actaatccag cattaagcta cgaaaggccg atatcctcgg attgtttcac gtttttctgc
     1021 aaagctgtca ccgggccctc ctcctgaaca gccaattcct gaaggagtaa gaaccatatg
     1081 ttctcgaaag aaggtaaagc taaggatatt agaacgccaa agatctgcga cgtcgtttgg
     1141 cacaatggtg agtttattaa atggagcgac gcgcgcgttc atgtgatgtc tcacgtgctc
     1201 cactatggat caagcatttt tgagggcatc aggtgttact cgacagaaaa agggcccgcg
     1261 atctttcggc ttcgcgagca catgcaacgg cttttaaatt ccgcaaaaat ctatcgtatg
     1321 gaacacgagt ggactttaga ccaactgtgc gatggtgctg ttgagttagt ccgccgaagc
     1381 cagatggatc aatgctacat ccggccaatc ctgttccgca gtcttgacga agaaaatcca
     1441 gcctttggag taaatccttt tccgaatcct ttagcgtgct atatcggcgc ctgggactgg
     1501 ggaaaatatc tcggcgacga agcgattgaa caaggtgtcg atgtttgcgt ctcgacctgg
     1561 aacagactga ctcctaactc gatgccggcg atggcgaaga gcggcgcgaa ctacatgaac
     1621 tcgcagctga tcaaaatgga ggcactcctc aacgggtttg ctgaaggcat cgcactcgac
     1681 gatcgcggtt atgtttctga aggttcggga gagaatatct tcctcgttgc gggcgatgtc
     1741 gttttgaccc cgccattgtc atcttcaatt cttcccggta tcactcgaga tagcgtgatt
     1801 cagatctgtc agcagctcgg aattacggtc aaagagagga caattcaacg cgcagctttg
     1861 tatgtcgcgg acgagctctt cttttcaggg actgcggcag aaatcacgcc aatccgttca
     1921 gttgaccgga ttacagtcgg caagggtggc aggggcgaaa ttacggaaaa aatccagaag
     1981 actttcttcg agattaccaa agctaaacgt ccagctcccg gagactggtt gacctatgtc
     2041 gaccgcaagc gtcaatcgga cgacaacgac gcgcaatcgg gaaacattga gagttctgga
     2101 tctcacgacg gggaaccagc gcttactctt caggcgcttt cgagcgacta gtccgccata
     2161 gaggcaaaat cagatcccgc accgaacgct aagttttggt gcgggatttt ttttcggagc
     2221 agctttgcct caaaagtcga tttaggtgct aaggagtttc gtgtttagtt cgtgtgactt
     2281 cgtggatcgt tctt
//
LOCUS       DASSFP010000215         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_649733, whole genome shotgun sequence.
ACCESSION   DASSFP010000215 DASSFP010000000
VERSION     DASSFP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649733"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1887
                     /locus_tag="VFH91_04655"
     CDS             241..1887
                     /locus_tag="VFH91_04655"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET6862309.1"
                     /translation="MKVTIGQINTTNGDFEGNTASIIRAIEQGKKDGSELVVLPEACV
                     QGYTSLDWFLDRDVQRCALQTLDKIVEATQGITAIVGTVRPSFLKTGRRLYNSAAVIR
                     NQELLGFSDKTLLPEYDVFDDPRYFQPANERHLFQVDDSNLGVAVCEDFWNDKTFWKE
                     RLYANDPADELIDLGAQVLVSINASPFNKGKMGQRCAMVSHRSKAAKMPIVFVNLVGA
                     NDGVIFDGASIVTNANGEIILQAPAFQEFIGTVDLDTKSIPQSCLPGDDIETIHQALV
                     LGIRDYAGKNGFQQVVLGLSGGIDSAVVAALACEALGPENVLSVMMPSPFSSRSSIDD
                     SVQLGRELGMRVIERPISKAFEVLLAEMDLQAPSEKHKSLAAENLQSRLRGNILMTIS
                     NAEGRLLLSTGNKSELALGYCTLYGDTNGGLAVIGDVLKTEVYELAKRLNIPKVIIDK
                     RPSAELAPNQFDDQSLPPYDQLDKILRLYFERKATPQEIIDTGFESQFVLDILNRVES
                     PANEFKRRQLPPTLIISRNAIGIGRRRPVTHKYRRERLNC"
     gene            1947..2942
                     /locus_tag="VFH91_04660"
     CDS             1947..2942
                     /locus_tag="VFH91_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862310.1"
                     /translation="MNCSRKKAAAARHTPSRILFCLFVVTCSFVVVQAQTSSNDNSPE
                     MNALSIVIRASNPTTKLAAAEDFLSRYPKSKNRIKLAQMVSEEIRKVPNPSAALTLLD
                     RAATIFTGADETRFFTTIRLDALLDSGQTDDGFRLATQVLKDTPDDVSTWIKLTFAGA
                     EEAKRRNLKYADLSLEYGTKAIRLIEMNQPPAGVDEQEWTNLRSELGALYQATALLYL
                     AQENTSEASSRVARAIATSPGDPANYALLGRLLQTDYERNEQDSNSRSSPIQHDPVDQ
                     IIEAYARAAGLAMGRIEYQKLLQQVIPDLTRYYKIRHNDSTQGLQQLIDRYRRVP"
BASE COUNT          856 a          797 c          798 g          761 t
ORIGIN      
        1 cgtctcactt tgtcagatct taaattctaa tgagtgcttg attcgtttag gttcagcggg
       61 ggcggcgctg gatctcacaa tggaccttga atctaattgc cgctctgccc ccatcccaac
      121 tgcatgattc tggaaatgtt ctgattgcga agttagaaaa atgaccaatg acaaatgatt
      181 tcgggttgcg atcccataag gaaaaaggca cgatccagcc gaccaaaacc ccattaatct
      241 atgaaagtca caatcggaca aatcaatacc accaacggag actttgaggg caacactgcc
      301 agcatcatcc gcgcgataga acagggaaaa aaggacggct cggaacttgt cgtgctcccg
      361 gaagcctgcg tgcagggtta cacgtcgctc gactggtttc tcgatcgcga tgtgcaacgt
      421 tgcgctctac aaacacttga caagattgtc gaggccactc aagggatcac cgctattgtc
      481 ggcaccgttc gtccctcgtt cttgaagacg ggtcgacgtc tttataattc cgctgcagtg
      541 attcgcaacc aggagctact tggcttctca gataaaacat tgttaccgga atacgacgtc
      601 ttcgatgatc cgcgttactt ccagcctgca aacgagagac atctattcca ggtcgacgac
      661 agcaatcttg gagttgcagt ttgtgaagac ttttggaatg acaaaacctt ctggaaggaa
      721 cgcctttacg caaatgaccc ggctgatgaa ttgatcgacc ttggcgcaca agtacttgtt
      781 tcaataaacg cgtcgccctt caataaaggc aagatgggcc aacgttgtgc catggtttca
      841 caccgttcga aggcagcaaa gatgccaata gtgtttgtga acctggtggg agcaaatgac
      901 ggcgtgattt ttgatggcgc cagcatcgtc actaatgcca acggcgaaat aattttgcag
      961 gcaccggcat ttcaagagtt catcggaacc gtcgatctcg acacgaagtc gataccccag
     1021 agttgtttac cgggcgacga tatcgaaacc atccaccagg ctctcgtact gggtattcgg
     1081 gattacgccg gcaagaatgg attccagcaa gtcgtacttg gcttgtcagg aggaattgat
     1141 tccgcggttg tcgcggcttt agcctgcgaa gctcttggtc cagagaatgt cctgagcgtg
     1201 atgatgcctt cgcctttttc gtctcgttcg agtatcgacg attcggttca actcggacgt
     1261 gaactcggca tgcgggtaat tgagcgaccg atatcaaaag cgttcgaggt gttgttagca
     1321 gagatggatc tgcaagctcc ttctgaaaaa cacaaatcac tggcagctga aaatctccaa
     1381 tcccgcttgc gtggaaatat tctgatgacc atctcgaacg ctgagggccg tttgttattg
     1441 tcaaccggga acaagtcgga actggcactt ggttattgca cgctttatgg tgacacgaat
     1501 ggtgggctcg ctgtaattgg agacgtacta aagacagaag tttatgagct cgccaaacgg
     1561 ctaaatattc ctaaagtaat tattgataag cgaccatctg cagaactcgc gccaaatcaa
     1621 tttgacgacc aatcactgcc accatacgac cagctggaca agatcctgcg gttatacttc
     1681 gagcgtaaag ctacaccgca ggagatcatc gataccggat tcgagtcgca gtttgtgctc
     1741 gatattttga atcgcgttga atcaccggcg aacgagttca agcgccggca gctgcctcct
     1801 actttaatca tctcgagaaa tgcaatcggc atcgggcgcc gtcgcccggt aacgcataag
     1861 taccggcgcg aacgtttgaa ttgttaactt tggagtgcgc cggcccggcg gcgctttgtt
     1921 aatcgcgcaa cttccgaggt gttcctttga actgcagtcg taaaaaagcg gcggcagccc
     1981 gccacactcc aagtaggatc ctcttttgcc tatttgttgt tacctgctcg tttgtcgtgg
     2041 tccaggcaca aaccagctcg aatgataact ccccggagat gaatgcgctg tcgatcgtaa
     2101 ttcgggcatc gaatcccact acaaagcttg cagcggccga agatttcctt tccaggtatc
     2161 caaaaagcaa aaacaggatc aagcttgctc agatggtctc agaagagatc cgcaaggttc
     2221 ctaacccttc tgctgctttg acgctgctgg atcgggctgc gactattttt acgggtgcag
     2281 atgaaacacg atttttcacg acaatcaggc tggatgcttt actcgactcc ggacaaactg
     2341 atgatgggtt caggctcgca actcaagtgt tgaaagatac acccgatgat gtatcgacct
     2401 ggatcaagct gacgtttgca ggagctgaag aagcgaaacg aagaaatcta aagtacgcag
     2461 atctttcgct ggaatacgga acgaaagcta ttcgcttaat tgagatgaat cagccaccag
     2521 ctggagttga tgagcaggaa tggaccaacc ttagatcaga actaggcgct ttgtatcaag
     2581 ctacagcgct tctgtacctc gcacaggaga acacttccga agcaagctca agagtagcaa
     2641 gagcaatcgc cacaagccct ggtgaccctg ccaactacgc cctgcttggg cgactactgc
     2701 aaacagacta cgaacgcaac gagcaagact ccaattccag gtcgagcccg attcagcacg
     2761 atcctgtaga ccaaataatc gaagcctatg ctcgcgccgc gggtctggct atgggtcgga
     2821 ttgaatatca gaagttatta caacaagtaa ttcctgacct cacgcgatac tacaagattc
     2881 gccataatga ttcgacgcag ggattacaac aactcataga tagatatcga cgagtccctt
     2941 gagatacgag gtgcagctta agggaattta agataactct gcctgcgaca gttgacactc
     3001 cgtttttgct ggaccccatt tgaacatttg acgtggagca tggcatcctg aatgggaatg
     3061 gacgcgacca aaataaagac aaaactactg aggcatgtgg gtgaggcgat cagagatttc
     3121 aacatgatcc atgacggcga tcgggtgatg gtctgcatct ctggcggcaa agacagccac
     3181 acgctgctcg acttgttatt agaccttcag aa
//
LOCUS       DASSFP010000216         5723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_496961, whole genome shotgun sequence.
ACCESSION   DASSFP010000216 DASSFP010000000
VERSION     DASSFP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496961"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..704
                     /locus_tag="VFH91_04665"
     CDS             153..704
                     /locus_tag="VFH91_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6862311.1"
                     /translation="MSRENIQFRHDIDSSSESFSNKHKKLRALKKVSGKLKRILTGRD
                     HDGASKSNSPRILVVDDEESICFSMSEYFSLQGYKVDTANEIDEAEKLIESTPYRVVI
                     QDLRLTTARSPEGLEIIKFIRDVTPQTRVIVLTAYGSVEMEEEARRCGADAFLRKPKP
                     LSQVAQVVQGLLESPPKPQVRPA"
     gene            complement(715..3933)
                     /locus_tag="VFH91_04670"
     CDS             complement(715..3933)
                     /locus_tag="VFH91_04670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HET6862312.1"
                     /translation="MDPYIELHARSAFSFLEGASVPEELIATALAFEMPAMALLDRNG
                     VYGAPRFHLSAKRNGIKGHIGAEISVKDPRFKDSTSSVPLLVCTREGYQNLCRLITLM
                     KLRVPKHSKTGECVVTADELAAHHKGLICLTGGFDGPLAATLHNNPAAGSHQAQKTID
                     WLTTLFGRDHVYAEIQRHFNREEEARNQIVIELARRNHLPLLATNGVSHATEVRREVA
                     DVFTCIRNHVQLETAGRLLAENSERYLKPGPTMQSLFADLPEAISNTSQLSSRLEFTL
                     EDLGYEFPKYPVPGGETMMLFLRHRTEEGARLRYTGSNGAPRYAQARNQLERELTLIE
                     KLGLEGYFLIVWDIVQFCRREGILVQGRGSAANSAVCYSLGITAVDPIGMGLLFERFL
                     SEERGEWPDIDLDLPSGDQREKAIQYVYERYGKLGAAMTANVITYRGKSAAREVGKAL
                     DFDEDTLSRLAGLVHTWEWKDPKDTTERQFREAGLDLRNPRIKKFFQLYQLVQDLPRH
                     LGQHSGGMVICQGQLDAVVPLEPAAMPGRVVVQWDKEDCADLGLIKVDLLGLGMMAVL
                     EDSIQLIRDSYNEEVDLAHLPPDDRAVYAALQKADTIGMFQVESRAQMSCLPRLRPEK
                     FYDIVVQVAIIRPGPIVGNMVHPYLKRRQGREAVTYAHPLLEPVLKRTLGVPLFQEQL
                     LKMAMICAGFSGGEAEELRRAFGFKRSEARMKEIEVKLRRGLLHNGIAGKTQDEIVQS
                     ISSFALYGFPESHAASFALIAYASAYLKCNYLAAFSAAILNNQPMGFYQPFTLIKDAQ
                     RHGLKVLPIDITCSNWLCTIEKELSPNDSSPAKLSLRLGLKYVKGLSQQSGMAIEKER
                     VIKPFASIDDLRNRVPELRKDELRKLSAVGALNFIQSHSPKLTLHRRDALWQVEKAVR
                     ASGELYETLAEQDKHSPLFPMTLPERIDADFRGTGLTIGKHPVAYHRNELNKLGALRA
                     IDIAKLRNGTSIRVAGWVIVRQRPGTAKGFVFLTLEDETGVSNIIVTPQIFDQNRSVL
                     VDHPFLLIDGTLQNQDNVVSVKARSVQPLAFKVAAAASHDFH"
     gene            complement(3949..5328)
                     /locus_tag="VFH91_04675"
     CDS             complement(3949..5328)
                     /locus_tag="VFH91_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862313.1"
                     /translation="MFACIYSPDVNEELTDFAYAFSPVVEHTKQNTVTLDVAGCELMF
                     GSPYGLAAEVAARAKRPRSEGGLGSRVNVAIAANPDTAIHVALHLPGISFVADGEELN
                     SLQEFLIESLEYSLVNIEAKQAEQILETLKLWGVQTFGAFAALPVTGVSERLGQEGVH
                     LQALAAGKTKRHLKSKQPAPVFSNAIELDHPITELEPLSFIYARLLNQLCATLNSYAL
                     ATNELLVVMTLEDKTVHQRKLSLPHPLLDHKVFLKLLLLDTEMHPPPAGVVGLTISCE
                     PVKPRVIQNGLFIPLAPAPDKLELTLARLAKLVGETNLGSPSLLDTYRPDAFIMKRFV
                     LPTKLDSRRRRKATNNRQISPTDQQRRVGFRMFRPPLRAVVQSERGWPAQISAWGKQR
                     SVYGKVVRLAGPWRTTGDWWRTDRWARDEWDVALEPATKNQTSETQSLYRIYRELNSG
                     VWFVEGNYD"
     gene            complement(5409..>5723)
                     /locus_tag="VFH91_04680"
     CDS             complement(5409..>5723)
                     /locus_tag="VFH91_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862314.1"
                     /translation="WWYRFRRTVENKPTSVIVISQEACTRSCALLTLELKATAEWSTA
                     GHIQNNVYEFSSTLKTEPSVRVTHANLLRGNEIKIERRRPIIPGCNESRFQVRSVASA
                     EY"
BASE COUNT         1505 a         1366 c         1342 g         1510 t
ORIGIN      
        1 ctttacgttt ctcagaaaac ggtgacaaca cttctgtgtt cgtcgctgat tctgcagcgc
       61 agcttcgata gcaaacaagc tgtaatcgag gttattgcgg cgtaattaag ttctaagtaa
      121 caggcgtgtt ttccggtgca accttgataa ttatgagtcg cgaaaatatc cagtttcgac
      181 atgatatcga ttcatcatcg gagtcttttt ctaacaagca caagaaactc cgagcgttaa
      241 aaaaagtatc gggcaagttg aaacggatct tgaccggcag agatcatgat ggagcttcga
      301 aaagtaacag tcctcggatt ctcgtggttg atgatgaaga atccatctgt ttctccatga
      361 gcgagtattt ctctctgcaa ggttacaagg ttgatacagc aaacgagatt gatgaagcag
      421 agaagttaat cgagtcaact ccgtatagag ttgtcatcca ggatctgcgc ctcacgactg
      481 ccagaagtcc ggaaggatta gaaatcatca agttcattcg agatgtcacg cctcaaacga
      541 gagttatcgt gttgacagcg tatgggtcgg ttgaaatgga agaagaagcg cgacgttgtg
      601 gagcggacgc ttttttgaga aaaccgaagc cgctttctca ggtagctcag gttgttcagg
      661 gcctgcttga atcacctcca aaaccgcaag taagacctgc ctgatgtaag ccaatcagtg
      721 gaagtcgtgt gaagctgcag ccgccacctt aaacgctaag ggctgtacgc tacgagcttt
      781 tactgacacc acgttatctt gattctgcag agtcccatct attagcaaaa aaggatggtc
      841 tacaagcaca cttctgtttt gatcaaaaat ctgcggagtg acaatgatgt tgcttactcc
      901 tgtttcatct tccagcgtga gaaaaacaaa tcctttagca gttccgggcc gctgtctgac
      961 aattacccaa cccgccacac gtattgaggt gccattccgt aacttggcaa tatcaattgc
     1021 acgtaaggct ccgagtttgt ttagttcatt acgatgataa gcgactggat gctttccgat
     1081 tgttaagcct gttccgcgaa agtcagcatc tattctttca ggcaatgtca tgggaaataa
     1141 gggcgagtgt ttgtcttgtt cagccagcgt ttcgtacaac tcgccggacg cgcgaacagc
     1201 tttttcgacc tgccataagg catcacgcct atgaagagtc agtttgggcg aatgcgattg
     1261 aataaagttt aaagcaccta ccgcactgag ttttctgagt tcatctttgc gtagttccgg
     1321 cactcgattc cgcaaatcat caatacttgc gaaaggttta ataacacgtt ccttctctat
     1381 agccattcct gactgttgac taagaccttt gacatatttc agacctaaac gcagtgataa
     1441 tttagcggga ctggaatcgt taggggagag ctctttttca atagtacaaa gccagttaga
     1501 acaggtgatg tcgatcggta aaactttgag tccgtgtcgt tgcgcgtcct taatcaaggt
     1561 aaaaggttga tagaacccca tgggctggtt attcagaatc gccgcagaga atgccgcaag
     1621 ataattacat ttgagatagg cgctggcata agcaatcaaa gcaaaacttg cagcatgcga
     1681 ttcaggaaaa ccatacagag caaatgaact gatggactgc actatttcgt cctgagtttt
     1741 tcctgcgatg ccattatgta aaagccctcg acgtaactta acttcaatct ctttcatgcg
     1801 ggcttcagaa cgtttaaagc caaacgcacg tcgcaactct tcggcctcgc ctccggaaaa
     1861 tccagcgcag atcatggcca tttttagaag ctgctcctga aaaaggggta ctcccaaagt
     1921 tctcttcaat actggttcga gcaatggatg ggcataagtg actgcctctc tgccctgtct
     1981 tcttttcaaa taaggatgga ccatattacc gacgattggt cccggacgaa ttatggcgac
     2041 ctggacaaca atgtcataaa acttctcagg ccggagtcgt ggcaaacagg acatttgagc
     2101 acgactttcc acttgaaaca ttccaatggt gtcagctttt tgcagtgccg cgtaaaccgc
     2161 ccggtcatca ggtggcaaat gggccaggtc cacctcttca ttgtaagaat ctcgaatcag
     2221 ctggatggaa tcttcaagga cggccatcat gcccaggccc agcaaatcga ctttgattaa
     2281 tccaagatca gcacaatctt ccttatccca ttgaactact actcgacctg gcattgcggc
     2341 tggttcaagt ggaaccactg cgtccagttg gccctgacag atcaccatgc caccggagtg
     2401 ttgtccgagg tgtcgcggta aatcctggac cagttgatag agctgaaaaa acttctttat
     2461 acggggatta cgcagatcta aaccggcttc gcggaactgg cgttcggtag tgtctttggg
     2521 gtctttccac tcccaggtgt gcaccagccc ggcaagacgt gaaagagtgt cctcgtcaaa
     2581 atcgagtgct ttaccaactt cccgagcagc agatttgcct cgataggtga tgacgtttgc
     2641 agtcatggcg gcgccaagtt ttccgtagcg ctcatagacg tactgaattg ctttttctct
     2701 ttgatctccg cttggtaaat cgagatctat gtcgggccat tcgcctcgct cttcagaaag
     2761 aaatcgttca aacagcaggc ccattccaat ggggtcaaca gcggtaatgc cgagcgaata
     2821 gcaaactgcg ctatttgctg cggagccacg accctgaaca aggattccct ctctccggca
     2881 aaactgaaca atatcccaaa ctattaaaaa gtatccttcg agccccagct tctcgattaa
     2941 cgtcaattct cgttcgagct gattacgcgc ctgagcgtag cggggtgcgc cattcgagcc
     3001 tgtataacgc agtcgagcac cctcttcagt gcgatgtctg agaaacaaca tcatggtttc
     3061 accaccggga accggatatt ttggaaattc atatccaaga tcctcgagag tgaattccag
     3121 tcgtgatgac agttgagaag tatttgaaat agcttccggc agatctgcaa aaaggctttg
     3181 catggttggt ccaggtttta aatatctttc tgaattctca gcaagtagac gaccggcagt
     3241 ttccagctgt acgtgatttc ttatgcaggt aaaaacgtcc gctacttcac gtctcacttc
     3301 ggtagcgtga gaaactccat tggtggccaa taagggcagg tgattacggc gcgcaagctc
     3361 aattactatt tgattccgag cttcctcctc tcgattgaaa tgacgttgaa tctccgcata
     3421 aacatggtcg cgtccaaaaa gtgttgtcag ccagtcgata gttttctgag cctgatgcga
     3481 gcccgcagcg ggattgttgt gcaaggttgc ggctagtggt ccatcaaaac ctccagttaa
     3541 gcaaattagt cctttgtggt gcgccgcgag ttcgtcagcg gtgaccacac actcgccggt
     3601 ttttgagtgt tttggcacgc gtagtttcat caaggtgatc aaacgacaga ggttttgata
     3661 accctcgcgc gtgcacacta ataatgggac tgaagaagtt gagtccttga aacgcggatc
     3721 tttgacactt atttccgcgc cgatatgacc tttgattccg tttcttttag cggacaggtg
     3781 aaaacgcggt gctccataaa cgccgttgcg gtccaacaac gccatcgccg gcatctcaaa
     3841 cgcgagggca gttgcgatca gctcttcagg aacggaagct ccttctaaaa aactgaaagc
     3901 tgaacgggca tgtaattcaa tgtatgggtc cacaattctt agccagcctt agtcgtagtt
     3961 cccttcaaca aaccagacgc cgctatttaa ttctcgataa atacggtaaa gactttgtgt
     4021 ttctgaagtc tgattcttag tagcaggttc aagtgcaaca tcccactcat cgcgggccca
     4081 gcgatcagtt cgccaccagt cacctgtcgt tcgccacgga ccagccaatc gcaccacctt
     4141 tccataaacg cttcgttgtt taccccaggc gcttatttga gccggccaac cacgctcaga
     4201 ctgaactaca gcccgcaaag gtggtctaaa cattcggaaa ccaacacgtc gttgctgatc
     4261 tgttggagat atttgccggt tatttgtcgc tttgcgacgc cgtcgagaat ctagcttcgt
     4321 cggtaaaaca aatcttttca tgatgaaggc gtcgggacga tatgtatcca gcaatgatgg
     4381 agagccaagg ttggtttcgc ccaccaattt tgccagtcgt gccagcgtga gttctaactt
     4441 atccggggcc ggtgcaagcg gaataaacaa tccgttttgt attacacgtg gtttcacagg
     4501 ctcacatgaa attgtcagcc ccacaactcc ggccggaggc ggatgcattt cagtatcaag
     4561 taacagcagt tttaaaaaca ctttgtggtc caaaagtgga tggggtaagg acaatttgcg
     4621 ctggtgaaca gttttatctt cgagtgtcat aacgactaat agctcgtttg ttgcgagggc
     4681 ataactgttc aatgtggcgc agagttgatt taacaagcgc gcataaataa atgacagggg
     4741 ctcgagctca gtaattgggt gatcaagttc aatggcatta ctgaaaacgg gggctggttg
     4801 ttttgatttc aggtgacgtt tagtttttcc ggcagcgagg gcttgaagat gaacgccttc
     4861 ctggcccaaa cgttcggaaa caccagtaac gggaagtgca gcgaaagctc caaacgtttg
     4921 cacaccccag agttttaaag tctcgaggat ctgttcggct tgtttagctt caatattgac
     4981 cagcgaatac tcaagagatt caatgagaaa ctcctgaagg ctgttgagtt cttcgccatc
     5041 tgcaacaaaa cttattcctg gtaaatgaag tgcgacatgt atggcggtgt cagggtttgc
     5101 agcaattgca acgttaactc gcgatccgag acctccttca gagcgaggtc tttttgctcg
     5161 tgcggccact tcagcggcaa gtccataggg tgaaccaaac attagttcgc aaccagcaac
     5221 atcaagagtt actgtgtttt gttttgtatg ctctacgaca ggcgagaaag catatgcaaa
     5281 atcggtcaac tcttcgttaa cgtcaggact gtaaatacaa gcgaacatga tcaatgacca
     5341 ataatttttt acggaccatt aatgaaaacc ctatataaga cctattggac ttataggacc
     5401 tattcttctc aatactcagc cgacgctact gacctcacct ggaaacgact ttcattgcat
     5461 ccaggaatta tcggcctgcg acgctcaatc tttatctcat tccctcttaa caggtttgca
     5521 tgcgtcactc gtacagaagg ctcagtcttc aatgtagaag agaactcata cacattgttt
     5581 tgaatgtgtc cggcagttga ccattccgca gttgccttta attcgagtgt aagcaacgcg
     5641 catgaacgag tgcaggcctc ttgagaaata acaattacgg aagttggctt gttctcgacc
     5701 gttcttctaa aacgatacca cca
//
LOCUS       DASSFP010000217         8182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_357403, whole genome shotgun sequence.
ACCESSION   DASSFP010000217 DASSFP010000000
VERSION     DASSFP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357403"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VFH91_04685"
     CDS             complement(<1..1551)
                     /locus_tag="VFH91_04685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6862315.1"
                     /translation="MKLAYFSPLGPQRSGISDYSEELLPHLAEGANITLFVDGFYPTN
                     RELTSKFEICDYKRDRSYLRHLDQFDAVIYHFGNDHRYHSGILDAMKYRSGIVVFHDY
                     ALQEFFLGLARDRNDLRLYLDEVEFAYGQLARDAAAESLARGTTPAMLAQPIEFPLNQ
                     RIARAAEGIIVHSEWSRSRFAALAQGVPAARISMPIKLPASAKASDSTTVRIATFGLI
                     TPGKGIEQTLRALSALKGTHQFKYSLVGETNAFFDVRELVRRYGMEDYVEVTGHISLD
                     EFKQRINQTDIALNIRERTVGETSASLCRLLSAGVCSIVAEAAWYAELPNDCVVKIPL
                     DGSADKLLLAYLSRLIEDAPLRNRIGENARHYAAEEFNAKHRAVDYLAFISKIIEGRS
                     RRDLIANITHELELMGINSGDNTLMTTVARDVAELFPLPTINDPALNGTAPSNGNHSA
                     SHLAQRTGRMARPTGIDYKTAAIDYSAQLDAERNHYLATKPFYNLANKPDKHHGDGMD
                     AETHRHFCD"
     gene            complement(1575..2117)
                     /locus_tag="VFH91_04690"
     CDS             complement(1575..2117)
                     /locus_tag="VFH91_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862316.1"
                     /translation="MKCRFVLLTAAVVVWSLFLACKSKEVGTPVNAPAASPTISFQTA
                     RALPDNGFKAEVSLVEPLPKLRTGQKETISIKVKNASDVTWWPIGGETTERKDNKYYI
                     AAGNRWLDKDGKLTTEMEGHNAIPKPLKPGEETDMTLLVTAPKTPGEYIFNLDLVQEG
                     VAWFEEKGSTTTKVKVTIVK"
     gene            2427..5078
                     /locus_tag="VFH91_04695"
     CDS             2427..5078
                     /locus_tag="VFH91_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862317.1"
                     /translation="MTTSANRTVQNSARTGSRALALVLSSVVVREFLVVLVFCLFTSL
                     VTWPYVTRLRDVVVDPGDPYLVAWILWWDYHQTFTDPLNLFQANIFYPLKYTLAFSEH
                     SYGIAMLFFPMFALGFRPLTVHAVALFFGFALSGYGAFRLGRTLTGSSAVGWVSGIIF
                     AFVPFRFNMMSQVPYVFSAWMPLVFEALILFVRERSIKRAVWLGVAFFLTGITTISWF
                     TFTLVPFAIYAALLLTRYRLWRDREFWQRGVGALAIAAFALVPFMLPYLRVSKLYGFK
                     RSIEEIKYYSAMPIHWLAVENRNRLWRGMGESIPDAWKFKLFPGLLPILFSIAALVLN
                     RDRDKQVSATESDKSSLRHWLPRLDLFIVFLIAVSLLAIGFDRSQAFFGLFKRLTSER
                     VLTLLTVVVVVRLCIAYPVQLRPLHRNLIESLRSPRRCDAFWLGSLLTVVGFCFSLGW
                     NFFFFRICYDILPIFRSMRFVARGSLLAYLGLALLAGLGVRELAARIEQRKLLRTSTV
                     YVIACALLLIELNTAPLRIERGAVFPDAVTLRLKQTQMRGGIVHLPVGGLFNYRYMLR
                     AADHEKPLIVGTSGFNSPVENRLEELTANGPISEEVMTLMESVPTSYLVIANQSIVPE
                     RVDDYKQFLVGQLNAGRLRFINRFDGHDDLYAVVKNEPQAQSEAPLMLDVTLRDWAAV
                     IKDDQVRLLGAPMPWSLKLFRLHIATTGAIPRYKEFMADEEALLGGVIVGMPGEAQHF
                     EESFQRLLDNWTKRDPFLAKLDKLKEAEYINRLIENAHLSVATEEREMMTNRLVKGEI
                     NRAAVLSQIIDDPRFIEAEKSKSLIVLHYFAYLHRNPDDPPDNNLYGLEFWIGDLERN
                     HDPAKISDAFKNASEHLQYEKPRAQSK"
     gene            complement(5086..6021)
                     /locus_tag="VFH91_04700"
     CDS             complement(5086..6021)
                     /locus_tag="VFH91_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6862318.1"
                     /translation="MYKNQRIAVVIPAHNEARHIANVVNTVPTFVDQIVVVDDSSSDE
                     TQSILSSLSIDRLSILRLEKNQGVGAATMSGYAKALEGGCDIVAKMDGDGQMAPEHLS
                     LLLDALVDQGYDYAKGNRFLAGESLQTMPRHRLFGNIILTFVNKLASGYWHIFDPQNG
                     YTAIKCETLRKLDLTRIHQRYFFENDMLIALNFHNARVRDVAIPARYRDERSGLNPFK
                     VSLTFPLLFIPRFWHRIYQKYVLRDFSPIALFLFMGLILTAWGVAFGAYHWILSILTE
                     HVATTGTVMLSVLPLILGFQLILQAIVLDIQESPR"
     gene            complement(6149..>8182)
                     /locus_tag="VFH91_04705"
     CDS             complement(6149..>8182)
                     /locus_tag="VFH91_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862319.1"
                     /translation="ISEEDKNRWLLVIVGLPLLAAYALRFPFPDSSFDVWGLRLFHGE
                     RALRGYLYWPGEFFPTAAPFNPTPDMITSIFRHALGYRLGTIVNLLVLIWTATFIDKL
                     LRPYVSGDKLRSVGVLLVVFAEQILFEINNYMPDLLALPLLLEATRLTLNAREKEFEP
                     RVALRVALLLGMAVGLKLSNGAVAVPIVIVWIWRALRFRTLNAKQLTLLTFAAAALFF
                     SPFLSFMIWVYRATGSPIYPLYNKVFRSPLYPPFIGWDDRWGGYGVWEILTWPVIMFF
                     QPVRASELPVYSGRLSLAFIVSVFCILFRRHLDQRTRLTTFIFVLGTLLWSVTMGYIR
                     YGLYLEVLSGILLIGVCALLLSQRVVTRWRTVLASTIAILMVAQMALATVYLLRTEWS
                     LRPTVFTSWPDYKRETQHLFWDRSIRALVEPDDVNALDNVDVWIVSGIKMAGLLPFLN
                     DKAPCVSVRFLGLLLAPANRAALAKTLEHYRDEQMWSMVLQSDYNDALFALHGAGLKV
                     VEIKEFNIPFFAKEDTVPTYFFRVVQEKTEIAQPPRTKLEDSSNVDTGAVTLTAMNPP
                     VVIGHDTDLKLYVNLMNSSQATLPSLTSSDGQKRLRLIAYWTKDGAQVGKSLDTSLPY
                     DFLPGESAILPLPIRTPTAAAEYTLSLKFVSEAESTIARTGNKTVDFRVQVVQ"
BASE COUNT         2005 a         1998 c         2076 g         2103 t
ORIGIN      
        1 atcgcagaag tgccggtggg tttcggcatc cattccgtcg ccgtgatgtt tgtccggctt
       61 gttagcgaga ttgtaaaacg gctttgtcgc caggtaatga ttgcgttcag catcaagttg
      121 agccgagtaa tcaatggccg cagtcttgta atcaattcca gttggccggg ccatgcgtcc
      181 cgttcgttga gcgaggtgcg acgctgaatg gtttccattt gaaggggcag ttccgttcag
      241 tgctgggtcg ttgatcgtgg gcaggggaaa caactccgcg acatccctgg caacggtagt
      301 catcaatgtg ttatcgccag agttgatacc catcaactcg agctcgtgcg tgatattggc
      361 gatcagatcg cgtcttgatc ggccttcaat gattttgctg ataaacgcca ggtaatctac
      421 ggcacggtgc tttgcgttga actcctcggc agcatagtgt ctggcgttct cgccgattcg
      481 atttcgtaac ggcgcgtctt caattaagcg cgagaggtaa gcgagtagaa gtttatctgc
      541 gctgccgtcg aggggaatct tcactacgca atcgtttggc agttcggcat accacgcggc
      601 ttcagcgaca atgctgcaaa cacctgccga taataagcga cacaaacttg cactggtctc
      661 tccaaccgtg cgttcgcgaa tgttcaaggc aatgtctgtt tggttgatcc tctgtttgaa
      721 ctcatcaagt gaaatgtggc cggtgacttc tacgtaatct tccattccat aacgtcgcac
      781 gagctcacgc acgtcaaaaa atgcattcgt ttcgccgaca agagagtatt tgaattgatg
      841 ggttcctttg agtgcactga gtgcgcgcag ggtttgctcg attcctttgc cgggtgtaat
      901 aagaccaaac gtagcaatcc taactgtggt ggaatcagat gcttttgcag aagccggtaa
      961 cttaatgggc attgagatgc gagccgcggg aacaccttgt gcgagagctg cgaatcttga
     1021 acgactccac tcagagtgga cgataatacc ttcagcagct cgcgcgattc tctggttcag
     1081 cgggaactca ataggctgag ccagcatcgc aggagtagtt cctcgtgcaa gactttccgc
     1141 agctgcgtcg cgagcgagtt gtccatacgc aaactcaacc tcatcgagat acaaacgcaa
     1201 atcgttccga tcgcgtgcta acccgagaaa aaattcctgc aaggcatagt cgtgaaagac
     1261 gacgatgccg ctgcgatatt tcatggcatc cagaatgccc gagtgatatc gatgatcgtt
     1321 tccaaaatgg tagatgacgg catcgaactg atccaagtgt cgcagatagg atctgtcgcg
     1381 cttgtaatcg cagatttcga acttcgaggt aagctcgcga ttggttggat agaagccatc
     1441 aacgaagaga gtgatgttgg caccttcggc caggtgagga agaagctctt cgctgtagtc
     1501 gctaatacct gaccgctgag gccctagagg actgaaatac gctagtttca ccgttttgtc
     1561 cgaccactaa ctattcattt aacgatcgtc acctttacct tcgtggtggt agagcccttt
     1621 tcctcaaacc acgccacgcc ttcctgaacc aggtccaggt tgaatatgta ctcgcctggt
     1681 gtttttggcg cagtaaccaa cagtgtcatg tctgtctctt cgccgggctt caaaggtttc
     1741 ggaattgcat tgtgaccctc catctcagtg gtcaactttc cgtccttatc gagccagcga
     1801 ttaccagcgg cgatgtaata cttattgtct tttcgttcag ttgtttcgcc gccgattggc
     1861 caccacgtta cgtcgcttgc gtttttgact ttgattgaaa ttgtctcttt ctgaccagtt
     1921 cgaagtttgg gcagtggctc gaccaaactt acttcggcct tgaaaccatt gtcgggcaac
     1981 gctcttgccg tttggaaaga aatagttgga cttgccgcag gcgcattaac aggagttcca
     2041 acttctttag atttacaagc tagaaaaaga ctccaaacaa ccactgccgc ggtcaacagc
     2101 acaaatctac acttcatatg aaaggttcct ctcgaaaagg tcgacagtca aacatgcgca
     2161 cgttctgaac cgtaatttgt ttctagaact gccgctctga ttactacctg gtcgattatg
     2221 ctattctccc gcagcgacag ctgctttctt aacactcgtt gccaacctct cgatctttag
     2281 atagaccatt atctagtcgg tcggatgatc gactgctgcg tccagttgag ggacatcctg
     2341 aatgatcgac ttgtcgttcc aattattagt agcgtagaac aacgatacca attgcgattg
     2401 gtcaaactaa cattgagcaa ccgggaatga caacttcggc gaatcgcaca gtacaaaaca
     2461 gtgccaggac agggtcacgc gccttagcgc ttgtgttgag ctcagtggtc gttcgagagt
     2521 ttttagtcgt cttagtattt tgccttttca cttcgctagt cacatggccg tacgtaacac
     2581 gattgcgtga tgtagttgta gaccccggcg acccttacct ggtggcatgg attctgtggt
     2641 gggattatca ccagacgttt acagatcctc ttaatctgtt ccaggcaaat atcttctatc
     2701 ctctgaaata cacgctggca ttcagtgaac acagctacgg gattgcaatg ctgtttttcc
     2761 cgatgttcgc gcttggattt cgtccactca ccgtacacgc tgttgcattg ttttttggtt
     2821 ttgcgctgag tgggtatgga gcttttcgac tcggccgaac gttgactgga tcgtccgcgg
     2881 tgggttgggt aagcggaata atattcgctt ttgttccctt tcgtttcaac atgatgtcgc
     2941 aggtgcccta cgtgttttca gcgtggatgc cgcttgtctt tgaagcttta attctttttg
     3001 tccgcgagcg cagtatcaag cgtgcagtct ggctgggcgt tgcgtttttc ctgacaggta
     3061 tcaccacaat ttcgtggttc actttcactc tcgttccgtt cgccatttac gcggctcttt
     3121 tattgacacg ctaccggctc tggcgtgacc gtgaattctg gcaacgtgga gttggcgcgc
     3181 tcgcaattgc agctttcgcc ctcgttccat tcatgctgcc gtacttgcga gtttctaagc
     3241 tgtacggttt taagaggagc atcgaagaga tcaagtatta ctctgcgatg ccaatacatt
     3301 ggttagcggt agagaaccga aacagattat ggcgaggcat gggtgaaagt attcctgatg
     3361 cctggaagtt caagttgttc ccgggactgc tgcccattct tttctcaatt gcggctttgg
     3421 tcctgaatcg tgatcgagac aagcaagttt cagcgactga gtccgacaaa tcctcactgc
     3481 ggcattggct cccacggttg gaccttttca tagtatttct gatcgcggtt tcgttgttgg
     3541 cgattgggtt tgatcgctca caggctttct tcggattgtt taagagactg acttcggaga
     3601 gagttctgac tttgttgacg gtagttgtag tggttcgatt gtgcattgcg tatcccgtgc
     3661 agttgagacc attgcaccgg aaccttattg agtcgctgcg ttcgcctcgg cgctgtgacg
     3721 ccttttggtt aggttctctg ctgacggtcg ttggattctg tttctcgttg ggttggaact
     3781 ttttcttttt tcgcatctgt tatgacatcc tgccaatctt cagaagcatg cgctttgtcg
     3841 cacgtggttc cttgctggct tatctgggtt tggccctctt agccggactc ggtgtccgtg
     3901 aattggcggc aagaatcgag cagcggaaat tattacgaac gtcaacggtt tacgtaatcg
     3961 cgtgcgcttt gcttttgata gaactcaata ccgcaccact gcggatagaa cgcggtgcgg
     4021 tctttccaga tgcagttact ctgcgtttga agcagacgca gatgagagga ggcatagttc
     4081 accttccggt tggtggactt ttcaactacc ggtacatgct gcgtgccgct gatcatgaga
     4141 agccactgat tgttggcacc tcagggttca actctccggt agaaaacagg ctggaagaac
     4201 tcaccgccaa tggaccaatt tccgaagagg tgatgacttt gatggagtcg gttccaactt
     4261 cgtacctcgt cattgctaat caatccatcg ttccagagag ggttgacgac tacaaacagt
     4321 ttcttgtcgg tcaactcaat gccggacgcc ttcgcttcat caatcgcttt gatggtcatg
     4381 acgatttgta tgcggtcgtg aaaaatgaac ctcaggcgca aagcgaagct cccttgatgc
     4441 tggatgttac gttgcgggat tgggccgcgg tcatcaagga tgatcaggta aggcttttgg
     4501 gcgccccaat gccctggagt ctgaaacttt ttcgcttaca catagctacg accggcgcga
     4561 tacctcgtta taaagagttt atggctgacg aggaagcgtt attgggaggc gtaatcgttg
     4621 gcatgccggg agaggcccag cacttcgagg aaagtttcca gcgtctgttg gacaattgga
     4681 ccaaacgcga tcctttcctt gcgaagctcg acaagcttaa agaagcggaa tatataaatc
     4741 gacttatcga gaacgcacac ctcagtgttg ctacggaaga acgcgaaatg atgacgaatc
     4801 gtcttgtaaa aggagagatc aatcgcgcgg ccgtactttc gcagatcatc gatgatcccc
     4861 ggtttatcga ggccgagaag tcaaagtcgt tgatcgtact tcactatttt gcgtacttac
     4921 atcgcaatcc agatgatccg ccggataaca atctatatgg actggaattt tggataggcg
     4981 acctggaacg taaccacgat ccggcaaaga tttccgacgc gttcaaaaac gcatctgaac
     5041 accttcagta tgagaagcca cgcgcgcagt cgaaatagaa cagggttatc tgggtgattc
     5101 ctgaatatcc aacacgatcg cttggagaat cagttgaaaa cccagaatta acggcagaac
     5161 ggaaagcatc accgtaccgg ttgtcgcgac gtgttctgtc agaatactca ggatccagtg
     5221 atatgccccg aatgcgactc cccacgcagt cagtatcagg cccataaaca gaaacagcgc
     5281 tattggtgag aagtcgcgca aaacatattt ctggtagatg cggtgccaga atcgtggaat
     5341 aaagagaagc ggaaatgtca ggctcacctt aaatgggttc aacccggaac gctcgtcgcg
     5401 gtaacgtgca ggaatcgcca catcccgaac tcgcgcgttg tgaaagttga gcgcgataag
     5461 catatcgttt tcaaaaaagt agcgttggtg aattcgagtg agatcgagct ttcgcagagt
     5521 ttcgcacttg atggcggtgt acccattttg cggatcgaag atgtgccaat atccagacgc
     5581 gagtttgttg acaaaggtga gaatgatatt gccgaagaga cgatgtcgag gcatcgtttg
     5641 caacgactca ccagccaaaa accgatttcc cttggcgtaa tcgtaaccct ggtccaccag
     5701 cgcatcgagc agcagcgaca ggtgttcggg cgccatttgt ccgtcgccgt ccatttttgc
     5761 aacgatatcg cagccacctt ccaacgcctt tgcatatccc gacatagttg cggcaccgac
     5821 accctggttt ttctccaatc gtaaaattga caggcgatcg atgctcaggc tcgacagtat
     5881 cgattgagtt tcgtcggagc tggagtcgtc taccacaacg atttggtcca caaatgtggg
     5941 caccgtgttc acgacgttgg cgatatgacg cgcctcgtta tgagccggga tgacaacggc
     6001 gatgcgttgg ttcttataca tgagtaactt ttgccaaaaa gcgacttctt atattgcttt
     6061 tctggcttcg tcgcaaatca aaggaccagc cgagcgtgac ttgagggtgc ccacggaggg
     6121 acactcctac acattaagac ccttgatatc actgtacgac ttgaacccta aagtcgacgg
     6181 tcttatttcc tgttcgtgca atagttgact ccgcctcaga gacgaacttc aaactgagag
     6241 tgtattccgc tgctgccgta ggagtgcgaa ttggtaaagg cagaattgcg gattcgcccg
     6301 gaagaaagtc ataaggaaga gaagtatcaa gactcttgcc tacctgtgca ccgtctttgg
     6361 tccaataggc gataagtcgg agccgttttt gaccatcgga agatgttaat gagggtagtg
     6421 tcgcctgact cgaattcatc agatttacat agagttttaa atcagtgtcg tgccctataa
     6481 cgaccggggg attcatagca gtcagcgtta ccgcaccggt gtccacattg gacgagtcct
     6541 caagtttggt tctggggggc tgtgctatct ccgttttttc ctgaacaact cgaaagaaat
     6601 atgtcggtac ggtgtcctct ttggcgaaaa acggaatatt gaactctttg atttcaacta
     6661 ctttcaagcc ggctccatga agagcaaaca acgcatcgtt atagtcagat tggagaacca
     6721 tggaccacat ctgctcatct cgataatgct cgagtgtctt cgccaaagcc gcacgattag
     6781 caggcgcaag taacaatcct aaaaacctaa cgctaacgca cggcgccttg tcgttgagaa
     6841 atggaagtag ccccgccatc ttgattccgc tcacgatcca tacgtcgaca ttatccaatg
     6901 cgttgacatc gtcaggctca actagtgctc gtatcgaccg atcccagaac aagtgctgtg
     6961 tttcacgttt gtaatctggc cagctcgtaa agactgttgg tcttaagctc cattcggtgc
     7021 gcaacaaata aacagtagct aacgccattt gggccaccat caaaattgcg atagttgacg
     7081 ccagcacagt tcgccacctg gttacgactc gctgagaaag aagtaaggcg cagacgccga
     7141 tcagcaggat tcccgacagc acttcaagat aaagaccgta tcgaatgtat cccatcgtca
     7201 cgctccaaag gagtgtgccg agtacaaaga taaacgtggt caatcttgtg cgctgatcca
     7261 ggtgacgtct gaataaaata caaaagacac taacaatgaa tgccagcgac agtctgccgg
     7321 aataaactgg aagctctgag gcccggacag gttggaaaaa cattattacg ggccatgtca
     7381 gaatctccca cactccataa ccaccccagc gatcatccca gccgatgaag ggtggataga
     7441 gtggagaacg aaacacttta ttgtagagcg gatagatcgg actaccagtg gcgcgataga
     7501 cccagatcat aaacgagaga aatggcgaaa agaaaagtgc ggccgcggcg aaagtaagta
     7561 aggtcaactg ctttgcattg agcgttctga atcgtagcgc gcgccagatc caaacgatca
     7621 cgatgggcac agcaaccgca ccgtttgaaa gttttagtcc gactgccatt cccaacaaca
     7681 aggccacacg caatgccaca cgcggctcaa actccttttc gcgagcgttt aatgttaagc
     7741 gagtcgcctc gagaagaagt ggtaacgcca acagatcagg catgtagttg ttgatttcga
     7801 aaagtatctg ttcagcgaaa actacgagta agacaccaac tgacctcagc ttgtcgccac
     7861 ttacatatgg acgtagaagt ttgtcgataa acgtagccgt ccagatgaga acgagcaagt
     7921 taacaattgt gccaagccgg tagccgagcg cgtgacggaa aatgctggta atcatgtcag
     7981 gcgttggatt gaagggcgcg gcagtaggaa agaattcgcc cggccagtaa agatagccgc
     8041 gcaaagctcg ctcaccgtgg aaaagtcgta ggccccagac atcgaaggac gaatccggga
     8101 agggaaatcg caacgcataa gctgcgagca gaggcaaccc aacaataacc aacaaccacc
     8161 ggttcttatc ttcttccgaa at
//
LOCUS       DASSFP010000218         4287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1543163, whole genome shotgun sequence.
ACCESSION   DASSFP010000218 DASSFP010000000
VERSION     DASSFP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543163"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            660..1913
                     /locus_tag="VFH91_04710"
     CDS             660..1913
                     /locus_tag="VFH91_04710"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6862320.1"
                     /translation="MASKMAPDLLNQLTNEFRGDSLNNIASAIGISPAKTQTALGGLV
                     PVILSSLAGKASTTDGAHEVIDVIRRNNLASVSLADVSRPEGVTSLVSTGRSLVDLAL
                     GGRVNSVTNWIATNTSVSNSSITSLASLCAPVVLGFLGRRLGSTGLNASSLSSLLGSP
                     SSYLQNAPAGLTSALSLGGASTATRRIADDIATAPAYATQPKRSPWGWLLPLLLGAAA
                     LGLLAYLFLRRQEPVTVATPQPAARTETAPAPAAPAPVVPTSSGANLGALIDTKLPNG
                     VNLHIPSNGIENKLLAFIGDSTKAVDQTTWFSFDRLEFETDSATLKPSSREQLSNVAA
                     ILQAYPQVDLKIGGYTDNTGDPARNFKLSQDRATSTLNELVGLGVAKSRLAAEGYGQQ
                     FPVADNATPEGRQRNRRIDIRVTKK"
     gene            2163..2366
                     /locus_tag="VFH91_04715"
     CDS             2163..2366
                     /locus_tag="VFH91_04715"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET6862321.1"
                     /translation="MSVAKVTEIIASSPKGFDQAIQLGIERANKTLENVKGAWVKGQK
                     LVVENGKVTEYRVNLRVTFVIKD"
     gene            2415..3311
                     /locus_tag="VFH91_04720"
     CDS             2415..3311
                     /locus_tag="VFH91_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HET6862322.1"
                     /translation="MRLLSLFVYFLVLIAVIGNPVVSSAQERKMGGVGITIFSDQNFR
                     GKSSTFREDVPDLEPLGLNDKISSLRVGPGEQWEVCEHSNYQGRCVVVSGLEPDLRRN
                     SWNDLISSLRRVRGGRPPVPPSGGDYIVLFDQTNYRGNPTNYNGPYPGLSGRAQSVTI
                     GRGVWELCEGQNYSGRCVTLDRSVPDLRDYNLRNRVYSVRPVGSGAPQPPPSTGDWYV
                     VLFDQANYRGTPRNYNGAVSDLGRDGRRAQSVTIGSGVWQLCENSNFRGRCITFETSA
                     PSFPYFLKNHVSSLRPITRQPR"
     gene            complement(3555..3722)
                     /locus_tag="VFH91_04725"
     CDS             complement(3555..3722)
                     /locus_tag="VFH91_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862323.1"
                     /translation="MFTILAHESTVATGLATVLSRVVAQVDTDSGWVSLEINGRRYLL
                     GAASETLQGLW"
     gene            3756..>4287
                     /locus_tag="VFH91_04730"
     CDS             3756..>4287
                     /locus_tag="VFH91_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862324.1"
                     /translation="MLKLLKLEIEKLFRAKSFYLSFAVLSAVVALMLWGFYTYAARKT
                     GGQAVEQFKYTYESKQYFNGLTFALYSVLFSFTLLLPIFVSMSAGSQIAGEARSGTLR
                     MICTRPISRASLILSKFLVTALHAALLLAFFIVLNLVVGLIFVGWGNLELYPGPLNLV
                     DAPGKIMRDEALMRFAY"
BASE COUNT         1011 a         1144 c         1123 g         1009 t
ORIGIN      
        1 gggtgacgcc acaacggcga gtgcggcgag tgcaaagatt cgtttcatgg gattcgctcc
       61 tttcagctag gttgcaaact gatgttgcga aggggggaag ttctatgcgg gagttctatc
      121 taaagttcgc tggtaagtca acgatttgag ctggtccttt tcgagcttaa aaacttctcc
      181 tatcgttgtt ctcagactaa agcggatcgg ttagccttgc gtccggggat cctacggagt
      241 acgattccta caccgccttt ataaataccc gaagtggttc aatcccgact tgttttgtgt
      301 ggttcagaca ctcccccaaa tcaatcgccg gtagctctgt agactacagc taacatcgct
      361 aacggtcgcg ggctcggcgg tcacgatgca acacatcgcg ggagtttttt ttatgagctc
      421 acatcggctc accgctcgtt gccatctgct aacagtagtt tctgccctct ttccatctgc
      481 tccggtcgat acccgatcct ctgctccaat gaaagcagat gccccctcag actcaattat
      541 gcccccggat acatcaaaga aaacttcgca atgcaaggta actgctaact aacgttccgg
      601 cgtgttgacg tcttacgaca agacacttcg gcaagctcat tggaaggagg attatcccaa
      661 tggcaagcaa gatggctcca gatcttttga atcaacttac aaatgaattt cggggcgatt
      721 ccctgaataa tatcgcctcg gcaatcggca taagcccggc taagacacaa actgcccttg
      781 gcggcctggt accggtcatc cttagctcac tagccggaaa ggcttcaact accgacggcg
      841 cacatgaagt catcgatgtc atccgccgaa acaatctagc ttcggtgagt cttgctgacg
      901 tatctcgccc tgaaggagtt accagcctgg ttagcaccgg acggtcactc gttgatttgg
      961 ctctcggagg cagagtgaac tcagtgacga actggatcgc aacgaacacc agcgtcagca
     1021 actcgtcgat tacttcgttg gccagtctct gcgcgcccgt agtacttggg ttccttggcc
     1081 gacgccttgg cagcactggc ttgaatgctt cgagcctgtc cagtttgttg ggtagtccca
     1141 gctcgtattt gcagaacgcg cccgcaggac tgaccagtgc tctctcactc ggcggtgcgt
     1201 ctactgcaac gcggcggatt gcggatgaca ttgctactgc accggcgtac gccactcagc
     1261 cgaagcgttc accctggggg tggctactac cgcttcttct cggggccgct gcgttgggac
     1321 tactggctta tttattcttg cgcaggcagg aaccagttac cgttgctact ccccaacccg
     1381 cggcgcgcac ggaaacagcc ccggcgcccg ctgcacccgc gccagttgtg ccaactagtt
     1441 ccggtgcgaa tctgggcgcg ctgatcgaca ccaagctgcc aaacggagta aacctccaca
     1501 ttccctcaaa tggcatcgag aacaagttgt tggctttcat cggcgactcg accaaagcag
     1561 tggaccagac cacgtggttc agtttcgacc gcctggaatt tgagactgac tcggcaacgc
     1621 tcaaaccttc atcacgtgaa cagctctcaa acgtagccgc gatcctccaa gcctaccctc
     1681 aggtggacct gaagattggt ggctataccg acaacactgg cgatccggcc cgcaacttca
     1741 aactctcgca agaccgggcg acaagcaccc ttaatgaact cgtaggtctc ggcgtcgcaa
     1801 agtcgcggct cgcggctgaa ggttatggac agcagtttcc cgtggcggac aacgcgactc
     1861 cagagggaag gcagcgcaat cggaggattg atattcgtgt gactaagaag taactccgtt
     1921 ttgacaaggt cagcgaagat gaagacgcgg cagttaagga actcgcttca gtgcttggtc
     1981 tgactgcaaa ggcatctggc gcaagttaat gtcactggtg tttagcgacc gttgatggtc
     2041 catccgcgat ctatacgaca aggcgctggc gggcacctga agccttgtag atcatcagcg
     2101 ttgatacggc ccgattggca ggcggttcct ggtgacaacg acaacaaaga aaggagaaaa
     2161 atatgtcagt agccaaagta accgagatta ttgcttcatc gccgaaaggc tttgatcaag
     2221 ctattcaact tggcatcgaa cgagccaaca aaacgctgga gaacgtaaag ggggcctggg
     2281 ttaaaggtca gaagctagtt gttgaaaacg gcaaggtcac cgagtaccga gtcaatttga
     2341 gggttacctt cgtgattaaa gactgatttc aacgtgccac tgcgcagcag caaagaagga
     2401 ggaaaaaaac gaaaatgaga ttgctcagct tgtttgtcta tttcctggtt ttaatcgcag
     2461 ttatcggcaa cccagtggta agttccgctc aggaacgaaa aatgggcgga gtgggaatca
     2521 cgatcttcag cgaccaaaat tttcgtggca agagttcgac gtttcgtgaa gacgttcccg
     2581 atctcgaacc cctcggcctc aatgacaaga tctcaagctt gagggtagga ccaggagagc
     2641 aatgggaagt gtgcgagcac tccaactatc agggtcgatg cgtcgtagtt tccgggttgg
     2701 aacctgattt aagaaggaac tcctggaacg atcttatttc ttcattaaga agagtgaggg
     2761 gtggacgtcc gcctgtgcct ccgtccgggg gcgactacat agttctcttc gatcagacta
     2821 actatcgtgg taatcccacc aactataatg gaccatatcc gggtctttcg ggacgcgctc
     2881 aaagtgtgac tatcggaagg ggtgtgtggg aactctgcga agggcagaac tacagcgggc
     2941 gctgtgtcac gctggatcga agcgtgccgg atctccgaga ttataacctg cgtaatcgtg
     3001 tctattccgt gcgaccggtc ggatcaggag caccgcaacc gcctccatca accggcgatt
     3061 ggtatgtcgt gctcttcgac caggccaact atcgtggtac ccccagaaac tacaatgggg
     3121 cggtatcgga tctaggaaga gatggccggc gcgcgcagag cgtgactatc gggagtgggg
     3181 tatggcagct gtgtgaaaac agcaacttta gaggacgctg catcaccttt gagacgagcg
     3241 cgcccagttt cccctatttc ttgaaaaatc atgtttcttc actccggccg atcacccggc
     3301 agccacgcta agcttaaagg ggattgtgac aaggaaagat tttttcaacg ctgaaggagg
     3361 aaatgctggt tggcccaaca accaacagac tctgaagcac aatgttcctc cagtctaact
     3421 ggagaaagag aagaagcaga atcgagaact agaaagctct cccacacctt gtccttcgta
     3481 gtgtcctagc ttggctactc tgtgcaacct ttgtgtcctc tgtgcctctg tggagcaaag
     3541 tcccaagaaa accatcacca cagaccctga agagtctcag aggcagcacc gagaaggtat
     3601 cgtcggccgt ttatctcgag actcacccaa cccgaatcgg tatcgacctg tgccaccacg
     3661 cgactgagta ctgtggcaag tcccgttgcc accgtactct cgtgcgctaa tatggtgaac
     3721 aacagcttag ccgagcacgt taaacgttgg tggatatgct caaactactc aaacttgaaa
     3781 tcgagaaact gtttcgcgcg aaaagctttt acctctcatt cgctgttctg agtgcggttg
     3841 ttgcactgat gctttggggg ttctacacct atgccgcccg aaagacaggt ggtcaggccg
     3901 ttgagcagtt caaatacact tacgaatcga agcaatactt caacggacta actttcgcac
     3961 tctactcggt ccttttctct tttactttgt tactgcccat tttcgtgtcc atgagcgccg
     4021 gttcacagat cgctggtgaa gcgcgctcgg gcacgctgcg aatgatctgt acgcgtccga
     4081 tctctcgcgc ctccctgatc ctttcgaagt ttctcgtgac tgcacttcac gcggcccttc
     4141 tactggcgtt cttcatcgtt ttgaatttgg tggttggcct gatctttgtt ggctggggaa
     4201 acctggagct ctatcctggt ccgcttaatc tcgtcgatgc gcccgggaaa attatgcgcg
     4261 atgaagcgct tatgcgattt gcctacg
//
LOCUS       DASSFP010000219         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1996095, whole genome shotgun sequence.
ACCESSION   DASSFP010000219 DASSFP010000000
VERSION     DASSFP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996095"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VFH91_04735"
     CDS             <1..1314
                     /locus_tag="VFH91_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862325.1"
                     /translation="VMNKAVFILLALVVVPGAFSQSSSSNKQVSTSKPTTKSSPAANQ
                     EATPNPAEEKKAEPEVPVDPIADLRHKIESATDVEKLRLQLQLADQLATAGRQPEALS
                     ELVQAASTDIFDPTSFYNLGNSFARLGATDAAISAYRKAIDQRKGNYSKAYNNMGVIL
                     LRSGRWDEARQAFLSALKLESFHYAEASYNLGRLYAAQGQNDLATREWRRALSVDPGH
                     SAAAEALTRNSNAERIVVEPKPANEPKPKFTGPVKPQPANNVAAGSRSTRNLTIDSIS
                     FDYLQRARNASERGNNSDAIDNYHRLINRQGGYFAPANLEMSYALITLKRYDEALTNL
                     LQVVNNDGTRYPISYYHLARVYETKGDLKQAEQAFTEAAKAYGAENNQFLLDLSRVRE
                     KQGNYKGALEAMEQYVTQVEAKGLKYEWSDERLSTLRQKVATVPK"
     gene            1416..1898
                     /locus_tag="VFH91_04740"
     CDS             1416..1898
                     /locus_tag="VFH91_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6862326.1"
                     /translation="MTSRIPLALSVIVLFASIAFSQEATPSPSPAPKPAMTRAQSQRI
                     IISTEKKLWEGWKTKDLKVFKSNLAADSLMVGENGVVTKADSLKEMESMSCDVKSYAL
                     SDIKVTFLNNDAALMTYKGTQDATCGGQQVPAAVWASSIYVKRGGRWFAMSHQETPAK
                     "
     gene            complement(1998..3554)
                     /locus_tag="VFH91_04745"
     CDS             complement(1998..3554)
                     /locus_tag="VFH91_04745"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET6862327.1"
                     /translation="MSLPFLEENDQTIARREHLDALRQLVGNVYPNKFERSEIVAEGR
                     EDTISAIAEKFRAFEPKPTEGRPSTEEIELANEQLNSFSVRVAGRIATPPRTMGKAAF
                     VHLSDGVSRLQIYLRKADFVALSNDAGGGEVDGWAVFQLLDHGDFIGVEGYLFVTKTG
                     ELSVHVQKLQFLSKALLPLPDKMHGINDPEIRQRQRYADLIAGSLKLEKDEALTPREV
                     FDRRANAIKEMRRILEDHGYVEVETPMLTPLATGAAARPFKTHHNTLDIDLYARIAPE
                     LYLKRLLVGGFEKVFELNRNFRNEGVSARHNPEFTMLEFYWAYADVNQMMDFCEDLIR
                     AVVFTTTGHTTLKFGEDSLDFAEIFRRVGMKESLSSLVPAESIRNENVVELFEQHVEH
                     GLVQPTFVTDFPKPISPLSKASPANPAVAERFELYIAGMEIANGFSELNDPVEQFDRF
                     RDQVKQRERGDEEAMLMDEDYLRALSYGMPPAAGIGIGIDRLVMLLTNKQSIRDVILF
                     PHLRPEKKEN"
     gene            3705..4187
                     /gene="rfaE2"
                     /locus_tag="VFH91_04750"
     CDS             3705..4187
                     /gene="rfaE2"
                     /locus_tag="VFH91_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET6862328.1"
                     /translation="MNRKILTIEQLVNERERLRSAGARVVFTNGVFDLLHLGHVRYLA
                     SARALGSTLIVAINSDSMVRELKGPGRPVTNQAERAEILSALRAVDYVTVFDDLSPRS
                     LIARLLPDVLVKGGDYKLNEIHGREEVEAAGGKVVALPFVEGASTTAMIEKVKRTPSG
                     "
BASE COUNT         1149 a         1082 c         1061 g         1049 t
ORIGIN      
        1 gtaatgaaca aagcagtatt cattcttctt gctcttgttg ttgttcctgg cgcgttctcg
       61 caatcaagtt cctcaaataa acaggtttcc acgagtaagc cgactacaaa gtcttctccc
      121 gcggcaaacc aggaagcgac acccaatcca gcggaagaga aaaaagctga gcccgaagtg
      181 ccggttgatc ctatcgctga tctccgtcac aagattgaat cagccacgga tgtggaaaaa
      241 ctccgtctgc aactgcagct cgcagaccaa cttgcgacag ccggacgaca gccagaagca
      301 ctgtcggagt tggtccaggc tgcatctacc gacatctttg atccgacaag tttttataac
      361 ctggggaact catttgctcg acttggtgcg accgatgccg caatcagcgc atatcgtaaa
      421 gcgatagatc aacgtaaagg caattattcg aaagcctata acaatatggg cgtgattttg
      481 cttcgttccg gacgttggga cgaagcgcgc caggcattcc tgtccgcttt gaagctcgag
      541 agttttcatt atgccgaggc cagctacaat ctgggtcgtc tttatgctgc tcagggccag
      601 aacgatctcg caacgaggga atggcgacga gccttgtctg ttgacccggg acattcagca
      661 gccgcagaag ccctgacacg aaattccaac gcggagagaa ttgttgtcga gcccaaacca
      721 gcaaatgagc ctaagccaaa atttactggt ccagtcaaac cccaaccggc caataacgtg
      781 gcagccggct ctcgatcgac cagaaacctc acgattgatt cgatcagctt cgattattta
      841 caacgcgcgc gaaacgcctc ggagcgagga aataattcag acgctattga caattatcat
      901 cgcctgatta acaggcaagg tggctacttc gcgcccgcaa atcttgaaat gagctatgcg
      961 ttgattactc taaagcgcta tgacgaagcc ctcacaaatc tcctccaggt tgtaaacaat
     1021 gatgggacac gttatcccat cagttactac cacctggccc gagtctatga gactaaagga
     1081 gatctcaaac aagctgagca agcattcact gaagcagcaa aggcatacgg cgcagaaaac
     1141 aatcagttcc tcctcgactt gagtagagtc cgcgagaaac aggggaatta taagggcgcg
     1201 ctcgaagcta tggagcagta cgtcacgcag gttgaagcga aaggattgaa atacgaatgg
     1261 tcggatgagc gactttcaac attgcggcag aaagttgcca ccgtgccgaa ataacctctc
     1321 caggtccaat gcggtcattg acagacaacc gcggcggtag taaaaacacc gcgttttatc
     1381 tccaatacaa caacccaaca aaggagttga agtcaatgac atcaagaatc cccctggctc
     1441 taagcgtaat tgtcttgttt gcctcaattg cgttctctca agaagctact cccagtcctt
     1501 cgccagctcc aaaacctgcg atgacccggg cccaaagcca aaggataatt atctcaaccg
     1561 agaagaaatt gtgggaaggt tggaagacga aagatcttaa agtcttcaag tcaaacctcg
     1621 ctgctgacag tttaatggtg ggcgagaacg gagtcgtcac caaggccgat tcactcaaag
     1681 aaatggaatc aatgagttgt gacgttaagt cgtacgctct gagcgatatc aaggttacgt
     1741 tcctgaacaa tgacgcagcg ttgatgactt acaaagggac gcaagatgca acttgcggag
     1801 gtcagcaggt tcctgcggcg gtgtgggcca gtagtattta tgtgaagcgc ggcggaagat
     1861 ggttcgcaat gtcgcaccag gaaacgcccg ccaagtagaa gtaaggtccg atcattgatc
     1921 aattatcatt tggtgcgacg tttttccgaa aagaacccat aaaaaattga gaattgagaa
     1981 ttggtaatta tcaattctca attttctttt ttctctggac gcaggtgagg gaagaggatc
     2041 acatcacgaa tcgactgttt attcgtaagt agcattacta agcgatcgat gccgatgcca
     2101 atgcctgcag ccggaggcat gccataggaa agtgctctga gatagtcctc atccatcaac
     2161 atggcctctt catcgccacg ctctcgttgt ttcacctggt cacgaaaacg atcaaattgt
     2221 tccaccggat cgttcaattc agaaaacccg tttgcgattt ccattccagc aatataaagc
     2281 tcgaatcgct cggctacggc cggatttgcc ggagaggcct ttgaaagagg tgagatcggt
     2341 tttgggaaat ccgtaacaaa ggttggctgg accaatccat gttctacatg ctgttcaaaa
     2401 agttcaacga cgttctcgtt tctaatactc tcagcgggca ccagtgacga aaggctttcc
     2461 ttcattccca ctctgcgaaa aatctcagca aaatcaagtg agtcttctcc aaatttcaac
     2521 gtcgtgtggc ccgtggtggt gaaaacgacc gctctgatca gatcctcaca gaagtccatc
     2581 atctggttta cgtcagcata ggcccagtaa aattccagca tggtgaattc gggattgtga
     2641 cgagccgaga caccttcgtt ccggaaattt cgattgagtt cgaaaacctt ctcaaaaccg
     2701 ccgaccagta atcgctttag atataactct ggagcaatac gcgcatagag atctatgtcg
     2761 agcgtattgt ggtgagtctt gaacgggcgt gctgctgcac ctgtcgccaa cggagttaac
     2821 atcggagtct ctacttcaac atacccgtga tcttcgagta tccggcgcat ctctttaatc
     2881 gcgttggccc gtcgatcaaa aacctctcgc ggcgttaaag cctcatcttt ttccagtttg
     2941 agactgccgg cgatcagatc cgcgtaccgt tggcgctgcc gtatttcggg atcgttaata
     3001 ccatgcatct tgtcgggcag cggtaacagc gctttcgaga ggaactgcag tttctggacg
     3061 tggaccgaaa gttctcctgt ctttgtaacg aagagataac cttcaactcc tataaaatct
     3121 ccatgatcca gtagctgaaa aactgcccag ccatcaacct ctccaccgcc agcgtcgtta
     3181 ctcaaagcga cgaaatccgc tttacgcaga tagatttgta atcgtgagac tccgtctgaa
     3241 agatgaacaa aagcggcctt acccatcgtt cggggcggag tcgcgatccg gcccgccaca
     3301 cgaacactga aactgttgag ttgttcgtta gccagttcga tctcttcagt ggatggccgc
     3361 ccctccgtgg gctttggttc gaacgctcga aacttttcgg ctatcgcgct gatggtgtct
     3421 tctcgtcctt ccgctacaat ctcacttcgc tcgaacttgt ttggataaac attgccgaca
     3481 agttggcgca acgcatcgag gtgttctcga cgcgcgatag tttgatcatt ttcttcgagg
     3541 aagggcagcg acaatttgtt tcagtcctcc ggaacaggtc ggcgattata ggtatgcagc
     3601 gacgacagcg caattcaagt tgtctaaact aatctaactc aaaccgtaaa gtcctgatgg
     3661 tcatttcgag tacgcaatgg catcgtgcta gattctacct atccgtgaac agaaaaattt
     3721 tgactattga acagctggtt aatgagcgtg agcgtttgcg ttcggctggt gccagggtag
     3781 ttttcacaaa cggtgttttt gatttattgc acttgggcca cgttcgttac ctcgcctcgg
     3841 cccgcgcttt aggctcgacg ctaattgttg caatcaatag tgatagcatg gttcgagaat
     3901 taaaaggccc tggccgtccg gtcacgaatc aagcagagag ggcggaaatc ctttcggcgc
     3961 tgcgagctgt tgactacgtc acggtttttg atgacctatc gccgcgttcc ttgatcgcaa
     4021 ggttgttgcc ggacgtgctg gtaaaaggtg gcgactataa actcaatgag attcacggac
     4081 gtgaggaagt tgaggcggca ggtggaaagg tcgttgcgct gccattcgtt gagggtgctt
     4141 cgacaacggc gatgattgaa aaggtcaaac gaacgccttc aggttaatgg ttgccactcc
     4201 gcgcaacggg gtgatcaaag cggtcccgat tgcttaaagg ttggattaat cagcacttat
     4261 aattttcgaa agcatgaaca gcagtacaac atcggaaagc atacttcagt caactcttcg
     4321 gcgaatcacc aacactcctg a
//
LOCUS       DASSFP010000220         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_952609, whole genome shotgun sequence.
ACCESSION   DASSFP010000220 DASSFP010000000
VERSION     DASSFP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952609"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFH91_04755"
     CDS             <1..288
                     /locus_tag="VFH91_04755"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6862329.1"
                     /translation="YARRKQWPLEKVTVRLQQNQVHIKDCKECDQNSDGYVHRIERTV
                     SFEGNLAPEQISRLQEISHKCPVHRTLTSPIVIVDKQEEETLNDSPAGDFF"
     gene            complement(339..415)
                     /locus_tag="VFH91_04760"
     tRNA            complement(339..415)
                     /locus_tag="VFH91_04760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(379..381),aa:Pro,seq:ggg)
     gene            complement(568..1050)
                     /locus_tag="VFH91_04765"
     CDS             complement(568..1050)
                     /locus_tag="VFH91_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6862330.1"
                     /translation="MSRPAVAIPEKLFFKIGEVCDLAGVQAHVLRYWESEFPMLAPQK
                     NRAGQRVYRKRDVEMALRIKELLYEDQYTIAGAKKRLTNDLRGGSKLKIVGSEDDSAQ
                     PTLPVEFSTEDEFVPTATFTRDPIAPRTAEERQGLKRLAAELRELLSLLEKENQNRAK
                     "
     gene            1362..2531
                     /locus_tag="VFH91_04770"
     CDS             1362..2531
                     /locus_tag="VFH91_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6862331.1"
                     /translation="MTTSPTALRLAARLEGVGFSDIVKLRNRIMELRAQGAEVFQFEG
                     GEPFMPTPEPIKDAMMRALAENKTRYAASSGIAELRSAISDKLRSQNRIPAQDDDVIV
                     VNGGMQGLFGAFQSVVNPGDEVLLFSPYWTPIKDLVSHCQAKSVLVPTAEARERGFQQ
                     TLNRYVSGNTKAIYYNTPQNPSGVVFSSEEARAVAAFAQERDLIVIADEAYEDLVYDG
                     EHLSIASLDGMFERTITSFTFSKSFAMTGWRLGYAVAKEPWMTGLKKTILYSSNGVST
                     PTQWAGVAALGIEPEMRGRNKAAYRSRRDLLISGLNELGLFCEPPPGAFYAFPDVTRI
                     STDSRQAAEILLDRAGVATVPGIVFGPDGQGHLRFSFSTSIETIEAGLDSLRRHL"
     gene            2545..3612
                     /gene="thiL"
                     /locus_tag="VFH91_04775"
     CDS             2545..3612
                     /gene="thiL"
                     /locus_tag="VFH91_04775"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET6862332.1"
                     /translation="MKETDDKGPSQTISANNSHKRSSRSEFDFINQLRIGTATNPPSS
                     IIGDDAAVIKNSGEKETVITADLLVEDIDFRRTTTPPYLLGHKALAVSLSDIAAMGAR
                     PRWALVSIGIPLDVWDTNFVDKFYDGFFELAGRYDVQLIGGDTSRSPEKVVIDSIVLG
                     ESTTNGSIKRSGANAGDQVFVTGSLGAAAAGLRLIERGAHLADQNLGEAEAQVLDHLL
                     LRHLRPEPRVGWGMILGQERLATAMIDLSDGLSSDLNHLCRESGVGVVVDSTQIPIDT
                     QVRELCGRRALDPLQLSLNGGEDFELLFTVKPEKVSRLPRRVDGVGITYIGEIKPAAE
                     GINLREGSRVWELKPEGWEHF"
BASE COUNT          933 a          899 c          986 g          923 t
ORIGIN      
        1 tacgctcggc gaaagcagtg gcctcttgaa aaggtgaccg tgcgcctgca acaaaatcaa
       61 gtgcatatca aagactgcaa agagtgcgac cagaactctg atggctacgt ccatcgcata
      121 gaacgcactg tcagttttga aggcaattta gctccggagc agataagccg cttacaagag
      181 atttcgcata agtgtccggt ccatagaacc ctgacttcgc ctatcgtcat cgtagacaag
      241 caagaagaag aaacgctcaa cgattctccg gcgggagact ttttctaaac aagaagttaa
      301 aaagggcttt caacctttcg attggaagcc cttaaaaatg gtcgggacga ctggattcga
      361 accagcgacc tcacgcaccc caagcgtgcg cgctaccagg ctgcgccacg tcccgttggt
      421 gtcttagtat cctgccactg aagaaatgtc aaattgtagg ggcgttcctc cgtgggcgcc
      481 ccatttaatt cttcggtgat ttcccataac gacgaagggc gcccacggag ggacgcccca
      541 acaaattaga gaacgattta agccaagtta cttggcccga ttctgatttt ccttctccaa
      601 caaactgaga agttctcgaa gttcggcagc gagacgcttt aaaccctgcc tctcttcagc
      661 agtgcgcgga gcgatcgggt cacgagtaaa agtggcggtt ggaacaaact catcttccgt
      721 tgaaaattcg actggaagag tcggctgagc tgaatcgtct tctgaaccaa caattttgag
      781 tttacttccg ccgcgcaagt catttgtgag tcgcttcttc gctccggcga tcgtgtattg
      841 gtcctcgtaa aggagttcct tgattcggag tgccatctcg acatctcgct tccggtagac
      901 acgttggccg gcacgatttt tctgcggggc caacatcgga aactccgact cccagtaacg
      961 aagaacatga gcctgcactc cggcgagatc acaaacctct ccaatcttga agaagagttt
     1021 ttccggtatg gcaacagcgg gacggctcat atttatatcg ctatcgggga tcaaacggag
     1081 ctggctctct taagctggcg ttacgggagt atggatacct ttggcgagtt tgccagctaa
     1141 cttgtcgacg gcggacatgt tacccgtgct cactttcacg tgtcaatact tatacttagc
     1201 acggttcact tgaaacttcg tcttaaaagt attgtctgaa gtcagacggc gtaaagatat
     1261 gtcccggaag gtcgcaattt cgcctaattg gactgagttt aagtcaaaca gctatacttc
     1321 tatttcccac gaagcaacat tcggaaggat caatccaact agtgacaacc tcgccaaccg
     1381 ctcttcgatt agcagcacgc ctcgaaggtg tcggcttttc ggatatcgtc aagcttcgca
     1441 accgcatcat ggaattgcga gctcagggag cagaagtgtt tcagttcgaa ggcggtgagc
     1501 cattcatgcc gacgcctgag ccaataaaag acgcgatgat gcgtgcgctc gcggagaata
     1561 aaactcgtta tgcagcttcc tcaggcattg ccgaacttcg ctcagcaatc tccgataaac
     1621 tgcgaagtca gaatcggatt ccagctcaag atgacgatgt catcgtcgtt aacggtggaa
     1681 tgcagggttt atttggtgct ttccagtcag tcgtgaatcc aggtgatgag gtactcctct
     1741 tctcacctta ttggactccg attaaagacc tcgtatctca ctgccaggcc aagagtgttc
     1801 tggtgccaac tgctgaagct cgggagaggg gtttccagca aactctgaat cggtatgtca
     1861 gtggaaatac aaaagcgatt tactacaaca ctcctcagaa cccgtcaggt gtagtcttct
     1921 catcagaaga agccagggca gtggccgctt ttgcccagga aagagatcta atcgtgatcg
     1981 ccgacgaggc gtacgaagat ctggtctacg atggcgagca tctctccatt gcgtcgttgg
     2041 atggaatgtt cgaacggacg atcacgtctt tcacattttc caaatcgttt gccatgacag
     2101 gttggcgtct cggatacgcg gtggcgaagg agccgtggat gacgggactg aagaaaacca
     2161 ttctttactc ttcgaatggg gtgtccacac caacgcaatg ggccggggtt gccgcgctag
     2221 gtatcgaacc agagatgcgc ggaaggaata aggcggccta tcggtctcgt agagatctgc
     2281 tgatatcggg cttgaatgaa ttgggactat tctgcgaacc tccgccggga gcgttctatg
     2341 cgtttcctga tgttacgagg atcagcaccg atagccggca agctgctgag attttgttgg
     2401 accgtgcggg tgttgctaca gtaccgggga tagtatttgg cccagatggt caaggtcact
     2461 tgcggttttc tttttcaact tcgatcgaaa ctatagaagc tggtcttgac tcacttcgaa
     2521 gacatctata gataatgtgt agaaatgaag gagacagacg acaagggccc aagccagacg
     2581 atctccgcga ataattctca taagcgctct tctcgaagtg agttcgattt cataaaccaa
     2641 cttcggattg gaactgcgac aaatcctcct agctcgatca tcggcgacga tgctgcagtc
     2701 attaaaaact ccggcgagaa ggaaactgtc attacggcag atctgcttgt cgaggacatc
     2761 gactttcgac gaaccacaac tccaccttat cttttaggac ataaggcgct ggcagtttct
     2821 ttgtcggaca ttgcggcgat gggggcacgt ccgcgttggg cactcgtgtc gattggtatt
     2881 ccactggatg tttgggacac caattttgtg gataagtttt acgatggttt tttcgaacta
     2941 gccggtcgtt atgacgtaca gctgattggt ggagatactt ctcgtagtcc ggagaaagtt
     3001 gtgatcgatt cgattgtgct tggagagtct acgacgaatg gttccatcaa gagatcggga
     3061 gcaaacgccg gagaccaggt attcgtaact ggatctttag gagccgccgc tgcgggattg
     3121 cggctgatcg agcgtggggc acatttggcc gatcaaaacc ttggagaggc tgaggctcag
     3181 gttttggacc atttgctctt gagacacctg cggccggaac ctcgcgtagg atggggcatg
     3241 attctgggcc aggaacgact tgcaaccgca atgatcgatc tcagcgacgg tttatcatct
     3301 gatttaaacc acctctgtcg cgaaagtgga gtgggtgtcg tagtcgactc gacgcaaatt
     3361 ccgatcgata cccaggtaag agagctttgt ggccggcgcg cacttgatcc acttcaactg
     3421 tcgctcaatg gaggtgaaga ttttgagttg ctatttaccg taaagccgga gaaggtaagc
     3481 agactaccgc gtcgcgtgga cggagttggc ataacctaca taggtgaaat aaaaccagcc
     3541 gctgaaggta ttaacctccg ggaaggttca cgcgtctggg agttgaagcc ggaaggctgg
     3601 gaacattttt gattaagggc tttgcgcttt gtactttgtg ctttgtattt tgtactttgg
     3661 ccgtatgccc aacctaagca catcaaaaca aaggacaaag tgctaagcac aaagtacaaa
     3721 ccagcatgtt aaagtttcgg a
//
LOCUS       DASSFP010000221         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2139988, whole genome shotgun sequence.
ACCESSION   DASSFP010000221 DASSFP010000000
VERSION     DASSFP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139988"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFH91_04780"
     CDS             <1..797
                     /locus_tag="VFH91_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862333.1"
                     /translation="PAFGIKVITVFPDNPKLGKDAHQGAVLLFSTETGELLALMNASA
                     ITAQRTAAASAVATSLLAREEASELCIIGAGVQARSHLHALGCVRSIKRARIASKGFA
                     SARRLVEEMKDHCDFELTAVETAREAVQNADIIVTATNSQVPVIQLDWISAGSHINAV
                     GTHSPVSREIDGGTMAAARIFVDRRESAINESGDYILAEQEGLINRDSILGEIGDLIT
                     GKQKGRTERNEITLFKSLGLAIEDVVCADYLLRAAQKQRVGTFVEY"
     gene            complement(813..1544)
                     /locus_tag="VFH91_04785"
     CDS             complement(813..1544)
                     /locus_tag="VFH91_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862334.1"
                     /translation="MLETIGSMSTFAVLSLGLIFGLKHATEVDHVVAVSTIVSQHRNV
                     FRSAVIGGLWGIGHTASLLIIGALVLSLKVAIPERVSSWLEFAVALMIIGLGANALWR
                     ALKDGSHVHRHEHDHDGVAHVHVHFHDQETTHKPNRAHSHAVSGVGLKPVVIGTMHGL
                     AGSGALTLLVLTQIKSAWVGFMYLAIFGVGSIVGMLVMSGLIGLPFALSATRLTGLHH
                     RLQTAAACFSIAFGLWYAYSTAPTL"
     gene            complement(1538..2662)
                     /gene="hypE"
                     /locus_tag="VFH91_04790"
     CDS             complement(1538..2662)
                     /gene="hypE"
                     /locus_tag="VFH91_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET6862335.1"
                     /translation="MSSTFTPSPLFQRVERARRRKHKFTDTHITLAHGSGGRAMHELI
                     EGLFLEYLRNPLLDKLEDQAVLTPESFSNGTSTAGRRLAFTTDSYVVSPIFFPGGDIG
                     KLAINGTVNDLAMSAARPVHLSAGFILEEGFPVEELRRILASLRDAAAEANVAIVTGD
                     TKVVQKGGADKIFINTAGIGVIDTDVELGATRAQPGDKVILSGSLGDHGTTIMIARGD
                     LELETEIESDCAPLHALVADMVQQAESVSRLEGLRCFRDPTRGGLATTLNEIALSAEV
                     CIELSENLIPIREEVKGACEILGLDPLYVANEGKLVAVVSPDIAEALVACMKNNKYGV
                     DACIIGQVKAEPGGIVTMLTGFGGTRIVDMLVGEQLPRIC"
     gene            complement(2766..3887)
                     /gene="hypD"
                     /locus_tag="VFH91_04795"
     CDS             complement(2766..3887)
                     /gene="hypD"
                     /locus_tag="VFH91_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET6862336.1"
                     /translation="MKFIDEYRQSDLAIALAGKIASQTSRSLKLMEVCGGHTHTIFKY
                     GIEDLLPPNIEMIHGPGCPVCVIPLGRVDDAISIALQPDVIFTTFGDAMRVPGSKTSL
                     LDAKALGADVRMVYSPLDALKIARNNRDKQVVFLGLGFETTAPSTAMTILQAAKEDID
                     NFTVFCNHITILPALKAMLDSPDLQLDGFLGPGHVSTVIGTRCYDFVSRDYGKPIVVT
                     GFEPLDVLQSVYLIVKQIEEGRAEVENQYARVVNRDGNSKALEVLFEVFEPRDYFEWR
                     GLGSIPHSGMKLRKTFERFDAEAKFSVPGLRIADPKACQCGEILKGVKKPWECKVFGT
                     ACTPETPIDSCMVSSEGACAAYYNFGRLSKIASRSAAQS"
BASE COUNT         1040 a         1060 c          996 g          960 t
ORIGIN      
        1 ggccggcatt tggaatcaag gtaatcacag tctttcctga caatccaaaa ctaggcaaag
       61 atgcacatca gggcgccgtt ctcctattta gtactgaaac aggtgagttg ctcgcactca
      121 tgaacgcttc ggcaataacg gcccagcgta ctgccgccgc atctgctgtt gccaccagtt
      181 tgctggcgcg agaagaagcg tcggaactgt gcatcattgg tgctggcgtt caggctcgaa
      241 gtcacctgca cgcattaggg tgtgttcgtt cgatcaaacg cgcacgaata gcgagtaaag
      301 gctttgcgag cgctcgacgc ctggttgaag agatgaagga ccattgtgat ttcgaactga
      361 ccgcagttga aacggcaaga gaggctgttc aaaatgctga catcatcgtt actgccacaa
      421 attctcaagt gccggtcatt cagttggact ggatttcggc cggctctcac attaatgcag
      481 tcggaacgca ctcacctgtc tcgcgtgaaa ttgatggtgg gacaatggct gcggccagga
      541 tatttgtgga ccgtcgcgag tcggccatca atgaatccgg cgactacatt ctggctgagc
      601 aagagggcct gataaatcgc gacagtatcc tcggcgagat tggcgatctg atcaccggca
      661 aacaaaaggg tcgtactgaa agaaacgaga taacactatt caaatcacta ggtcttgcaa
      721 ttgaggatgt agtgtgtgcc gattatttgc tgagggcggc gcagaagcaa cgtgtaggaa
      781 cctttgtcga gtattaaccc ggacaccaaa ttctaaagtg ttggggcggt ggagtaggcg
      841 taccacagac caaatgcgat gctgaagcag gcggcggcag tttgtaagcg gtgatgaaga
      901 ccggtcagtc tcgtggcact taaagcgaac ggcagaccaa tcagtccgga catcaccaac
      961 atcccgacaa tcgatccgac cccaaaaatc gcgagataca taaaccctac ccacgcactc
     1021 tttatttgcg tcagcaccag cagagttaac gctcccgatc cggccaatcc atgcatagtt
     1081 ccaatgacta ccggctttaa gcccacacca gaaactgcat gtgagtgagc acgattcggt
     1141 ttatgtgttg tttcctgatc gtggaaatgg acgtgaacgt gcgcgacgcc gtcatgatcg
     1201 tgctcgtgac gatgcacgtg gcttccatct tttaacgcac gccaaagcgc attggcaccc
     1261 aacccgataa tcatcagagc gaccgcgaac tcgagccaac tgctgactcg ttcgggaatt
     1321 gccaccttga gagaaagcac aagtgctccg attattaaga gtgaagcggt atggcctatc
     1381 ccccacaatc cgccaatcac ggcggaacga aaaacattgc gatgctggct gactatcgtc
     1441 gatacggcaa cgacatgatc gacctccgtc gcatgtttca gtccaaaaat tagaccaaga
     1501 ctgagcacgg caaatgtgct cattgagccg attgtttcta gcatatgcgt ggtaactgtt
     1561 cgcccacgag catatcaact attcgcgttc caccaaaacc cgtgagcatg gttactatgc
     1621 caccgggttc agcttttacc tggccaatta tgcaagcatc aactccatac ttattgttct
     1681 tcatgcaagc aaccagagct tcggcgatgt ctggggagac tacggccaca agttttcctt
     1741 cgtttgctac gtacagagga tccagtccca atatttcaca agccccttta acttcctctc
     1801 gaatcgggat caggttttct gagagctcaa tacaaacttc ggcgctcaac gctatttcgt
     1861 tcagtgtcgt cgcaagcccg ccgcgagtag gatcgcggaa acagcgcaaa ccttccaacc
     1921 ttgatacgct ttcagcctgt tggaccatgt cggcaacaag agcgtggagt ggcgcgcaat
     1981 cactttcgat ctcggtttcg agttccagat caccacgagc gatcatgatg gtggtgccgt
     2041 gatcacccag agacccgcta agaattactt tgtcgccagg ttgagcacgc gtcgccccga
     2101 gctcaacatc ggtatcgata acaccaattc ccgctgtgtt gatgaagatc ttatccgcgc
     2161 cgcctttctg aacgacttta gtatcgccag ttacgatggc tacgttcgct tcagctgcgg
     2221 cgtctcgcaa cgatgcgaga atccttctta actcttcaac cggaaaaccc tcttccaaaa
     2281 taaatcccgc tgaaaggtga acaggtcgag cagcgctcat tgcgagatca tttacggttc
     2341 cattgatcgc tagttttccg atgtctccac ccggaaagaa gattggcgat acaacataag
     2401 agtcggtagt gaaggctaag cgtcgtcccg cggtcgacgt tccattcgag aacgactccg
     2461 gggtcaacac agcctggtct tcgagtttgt cgagtaatgg attgcgcagg tactcaagaa
     2521 acaatccttc gatcagttcg tgcattgctc ggccaccact tccatgcgca agggttatgt
     2581 gcgtgtccgt aaacttgtgc ttacgacgcc gcgcacgctc aacgcgctga aataacggtg
     2641 atggagtaaa ggttgatgac actggatgat ctttggtctt tggtcttcgg tcttcggtct
     2701 ttgtggaata ggccagcgaa actcaacgct caaagtacaa agaccaaaga tccaaagcca
     2761 aaggcctacg attgagctgc tgatcgtgac gcaatcttcg ataagcgacc aaaattgtaa
     2821 taggcggcgc acgcaccttc cgacgacacc atgcacgagt caattggcgt ttccggagtg
     2881 caagctgtac cgaaaacctt gcactcccag ggtttcttga cgcccttcag aatctcgcca
     2941 cactggcacg cctttggatc agcaatgcgc aaaccgggca cgctgaattt cgcttcagcg
     3001 tcaaatcgct caaaagtttt gcgcaatttc atcccactgt gcgggatcga gcctaagcca
     3061 cgccactcaa aatagtcacg cggttcgaag acttcaaaga gtacttcaag agctttgctg
     3121 ttcccgtcgc gatttacaac gcgtgcatac tgattctcaa cttcggcacg accttcttca
     3181 atctgtttga cgataagata aaccgactgc aggacgtcca gcggttcgaa accagtcacg
     3241 acaatcggct tgccgtaatc acgcgaaaca aaatcataac aacgagtacc gataacggta
     3301 ctgacgtgac ctgggccaag aaacccatcc agttgaaggt ccggcgaatc aagcattgct
     3361 ttcagggccg ggagaatagt tatgtggttg cagaaaacag taaagttgtc gatatcttct
     3421 ttcgccgcct gaagtatggt catggcggtg gacggtgcag tagtttcaaa ccccagaccc
     3481 agaaagacaa cctgtttgtc tcggttgttt cgggcaatct tcaacgcatc gagaggcgaa
     3541 tagaccattc ggacatctgc tccgagagcc ttcgcatcga gcagactggt tttggatcca
     3601 ggaacgcgca tcgcatcgcc gaaggtcgta aaaatcacat ccggttgcaa cgcgatcgag
     3661 atggcatcgt caacacggcc cagcggtatt acgcagacgg gacagccggg cccatggatc
     3721 atttcgatgt tcggaggcag cagatcctcg atgccgtact tgaaaattgt atgggtgtgg
     3781 ccgccgcaga cctccatcag ctttagtgac ctggaggttt gacttgcgat ttttccagcc
     3841 agcgctatgg ccagatcgct ttgtctatat tcgtcgataa atttcatcgc tccaagtcct
     3901 tccttaagag aagatcattt ctcatttctc atttctcata atttctcatt tctcattttt
     3961 ggatctcgag ggcctaagaa ttaattagta accgaatcag aaatgaaaaa tgggaaatga
     4021 aaaatgacaa atgaaaaatg atcttatttc tctttc
//
LOCUS       DASSFP010000222         7110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1544503, whole genome shotgun sequence.
ACCESSION   DASSFP010000222 DASSFP010000000
VERSION     DASSFP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544503"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFH91_04800"
     CDS             complement(<1..231)
                     /locus_tag="VFH91_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862337.1"
                     /translation="MPPSNILPTPHRPKGDMREMDITTTDSLKDELINRDPEFRELAR
                     EHHKYEERLSELSALSYPSDEEQLEEVTLKKKK"
     gene            complement(308..808)
                     /gene="rimI"
                     /locus_tag="VFH91_04805"
     CDS             complement(308..808)
                     /gene="rimI"
                     /locus_tag="VFH91_04805"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET6862338.1"
                     /translation="MTTAPTKSDTAIEECKIDRMSEHDLLEVVEIEEQSGLSRWGWAA
                     YHAELQSVDRSLLLVIRPSQEAAPAHRVVAYIVARETAGELHINNVAVRPEYRKRGLG
                     ACLLTRVLENGRNLGVSIAYLEVRASNLVARALYEKSGFRAIAQRPNYYTDPPEDAVV
                     MSLSLT"
     gene            complement(809..1516)
                     /gene="tsaB"
                     /locus_tag="VFH91_04810"
     CDS             complement(809..1516)
                     /gene="tsaB"
                     /locus_tag="VFH91_04810"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET6862339.1"
                     /translation="MSLQPLILSIETATTTGSIAVASGSLILSSKKGDGLSSHSNTLL
                     SDIDDMLRHLDRRFEEIDFFAAAIGPGSFTGLRIGIATIKALAMTANRPCAGIPTLEA
                     VARSAGENLCVAATLLAGRGEVFAQLFSVSAEGRVTEIDQPAHLPPREVLKKYQYLEN
                     LCWAGEGAEAHKAEILAFDDEVLKAGKKWRIASDLTNLAENVSALALQRSVTNELTSA
                     ENLHAIYVRPSDAELKK"
     gene            complement(1513..2637)
                     /locus_tag="VFH91_04815"
     CDS             complement(1513..2637)
                     /locus_tag="VFH91_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862340.1"
                     /translation="MIAANQAVAITPADAEAHRARAIVLDHLRLTGDGTKELEIAASL
                     RPRDDSIWLTLGNFRDFLDDSDGALSAYNQAVRFAPFYAHTNWARGNLKLRIGRYDEA
                     FSEMRLAATKNPNLLPTFIDLALGVSRGDLALAEQLVQITDDRSRLAFARTLATRGRG
                     EEALRQIQLLKTPLNRETKQELVRQLIAAKCYREGYELWNSDSTNKSLPVIVDGGFEG
                     SLTVKNTGFGWRFAAGEQKLEMSQDASERQSGTRSLRINFTGHLTPGNVLLSQYVVID
                     PDRTYKINYAFKSKELVSGGPPVITVDDVDSGETLGRSESIPNSTDGWQSASFTFTSR
                     SKSKAVAIVLRRNYCQTSPCPIFGVVWLDSFSIEALPKGP"
     gene            complement(2844..3521)
                     /locus_tag="VFH91_04820"
     CDS             complement(2844..3521)
                     /locus_tag="VFH91_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862341.1"
                     /translation="MSLKRRRGYQGIAALVLFSVAQVGVQVGFAEPTPGSPVVVPQQF
                     IAKLTTKNNQPITVNGLGAATGASIVTGAVIETGADQAATVNLGPLGSLDIAPNTKLV
                     LTYDENGNVKVTVLFGCAILSAKKKTTGEVGTEQGGTAGKTDPAKGGVLEICFPPGAA
                     APTVGPGVAAGAGAGAAGGAAAGGAGGAGGLFGLGTAGTVAILAGAGSAALIPFAFQD
                     NPSGAGL"
     gene            complement(3662..5611)
                     /locus_tag="VFH91_04825"
     CDS             complement(3662..5611)
                     /locus_tag="VFH91_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862342.1"
                     /translation="MKKTTKASLVAGLAAASLLISTPVPRVRGYSTSRWAMLRPLLPQ
                     DIGSGGFQGQDIMGGAAIIFKRPDRVRDLVGGAAMLVVKRQPRPTRQVEVARNTGRRP
                     TVRPGQTETGPTDSDKAEAFKNQGNTYYDVGKFADAIAAYQNALKLTPQDPVLYNNMG
                     AAYFSVNKNNEAAQAFNKSISIKPDDPDAYFNLGIAYSGMDKQQDALNAFKQAVRIKP
                     DWAEAQNALGDTYLALGQFSEAATAYRQTVRLQPNNATAYSNLGYALDRAGNSNESIE
                     ALRNSVKLKADDAVAYNNLGASLYKAGRYQEAIDAFSSAVRLKPDDADALNNLGAAHY
                     VVAQYDQALTNFQKAVAIRNDSPDANYNLGNAFYMTTRYREATTAYRRAIQLRSNYVE
                     ARTNLGSLLIALGEFREAITELNEAIRLKPDNPVSHNNLGYANVKLGETVDRTAAVPY
                     FQRAVESYKEAIRLRPDYIKALNNLGAVYNKLGQYQEAVTTLQRAVQLKPDFPEAQYN
                     LGTAFYNRNQFAEAVNALQQAVRLKADYTDAYNSLGSALYKNQQFDPAIEAYKKALAL
                     KPGSAETNNNVGTVYFRTKRYPDALASFKEAVRIKPDYGEAQFNLALTYVALNDRKNA
                     LDQYNRLKTIDPKLADDFFQRYLKK"
     gene            complement(5608..7023)
                     /locus_tag="VFH91_04830"
     CDS             complement(5608..7023)
                     /locus_tag="VFH91_04830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6862343.1"
                     /translation="MEDVAVVSTFAEAEQSSASRIADLLSKLIFGALLALIVIAALPY
                     GTAQPWWKALFVCGVFSICIVALIEHALNDSAKSKGMFLVVPLICLSIFAFLQTLPLR
                     RATPIEGLTSSPSFTAISADPYQTRFFALQLLALATVLALLYRYAATDSRIRWLVYVT
                     LSVATLSAIFGLVRQTTQHGTGFILPFLEPDQGYGQFINRNHFAYLMEMAFGLGLGIA
                     LAGGVKRDRVLLNVALLLPIWMALVLSNSRGGILAMLTQVILAALILVRGSRLSHVDQ
                     NGLTRIQSSRLFKTVLVVALVFGLVGGTIWVGGDPLVKSFQSASNELNPETQSAYSGA
                     SRNEIWRATLKMFTANPILGVGLGGYWIGITAYHDASGMVTPQEAHNDYLELLSSGGV
                     VGFALGLWFAITAFRKIKVALNTRSGFLRAVCVGAIMGIAGVAVHSLFDFGLHILVNA
                     LVLMVLLMMSTAEIRLESSSN"
BASE COUNT         1698 a         1826 c         1782 g         1804 t
ORIGIN      
        1 cttctttttc ttaagtgtaa cttcttccaa ttgttcctca tcactcggat aggaaagagc
       61 tgataattca ctaagacgtt cttcgtattt atgatgttcg cgtgctagtt ctctgaattc
      121 ggggtcccgg tttatcaact catctttcaa actatccgtc gtcgtgatgt ccatttcgcg
      181 catatctcct ttcggtcgat ggggagttgg caggatgttg gatggaggca agactttaaa
      241 acctccactt gattgattcg cacggaaaaa tggtagccca acaacaacac caaagcaagc
      301 tactaattca ggttaatgac agactcatta ccacggcgtc ctcaggaggg tctgtgtagt
      361 agttaggacg ctgtgctata gctcgaaaac cggatttttc gtagagtgcg cgcgccacga
      421 gattactggc acgaacctcg agataagcta ttgacacccc cagattgcgc ccattttcaa
      481 ggactctggt aagaagacag gcgcccaatc cccttttacg atactccgga cgcaccgcga
      541 cgttgttgat gtgaagttca cccgctgttt ctcgggcaac aatataggcc acaactcgat
      601 gcgctggggc ggcctcctgg gatggccgaa tgaccagcaa aagacttcta tcgactgact
      661 gtaactctgc gtgatatgca gcccatcccc agcgactcag tccggactgc tcctcgatct
      721 cgacgacttc gagtaggtcg tgctcagaca tcctgtcgat cttacactcc tcgatggcag
      781 tgtctgactt tgtaggcgcc gtcgtcatct atttcttcaa ctctgcatcg gaaggtcgca
      841 cataaatcgc atgaaggttt tccgctgatg tgagttcatt ggtaacggat ctttgtaagg
      901 ccagggcgct aacattttca gctagattag taagatccga agcgatgcgc cattttttcc
      961 cggcctttaa tacctcgtcg tcgaatgcaa gaatctcagc tttgtgcgct tcggctcctt
     1021 ccccggccca gcataagttt tccagatact gatacttctt gaggacttcg cgcggcggaa
     1081 gatgcgccgg ctgatcgatt tcagttacgc ggccctcagc tgagactgaa aataattgcg
     1141 caaagacttc accgcgccct gctagaagtg ttgcggcgac gcaaaggttt tctccggctg
     1201 atcgtgccac agcctccagg gttggaattc ctgcgcaagg tcggttcgca gtcattgcca
     1261 gagccttgat ggtggcaata ccaattcgca gaccggtaaa gctccctgga ccaattgcgg
     1321 ccgcaaagaa gtctatctcc tcaaacctgc gatctaagtg tctcagcata tcgtcgatgt
     1381 cgctaagtag cgtgtttgaa tgcgaactta gaccatcgcc ttttttgctg ctgaggatca
     1441 gcgaccctga tgcaaccgcg atgctccccg tcgtcgtcgc tgtctcgata ctgagtatca
     1501 gcggttgtaa actcatggcc ccttcggtaa cgcttcgatt gaaaaactgt ctaaccaaac
     1561 tacaccgaaa ataggacaag gagaagtctg gcaataattt ctacgcaata ctatcgcgac
     1621 agctttcgat tttgaacgag aagtaaatgt gaatgacgca gactgccagc cgtcagtaga
     1681 gttagggatt gattctgacc gaccgagagt ttcaccactg tcgacatcat ccactgtgat
     1741 cacgggtgga ccgcctgata ccagttcctt cgatttgaaa gcgtagttga tcttataggt
     1801 ccgatcagga tctatgacaa cgtattgaga cagtaagacg ttgccaggtg ttaaatgtcc
     1861 ggtgaagttg attctaagac ttcgcgttcc ggattgccgt tccgacgcat cctgagacat
     1921 ttcaagtttt tgctcgccgg cggcaaagcg ccagccgaat ccggtattct ttacagtcaa
     1981 actcccttca aaaccaccgt caactattac aggtaaggat ttgttcgtgg agtcactatt
     2041 ccaaagttca tagccttcgc gataacactt cgcagcgatg agctgacgga ccagttcctg
     2101 tttagtctcg cgattgagcg gtgtcttgag caattgaatt tgcctgagcg cttcttcgcc
     2161 gcggcctctt gtggcgagcg ttctcgcaaa tgcgagccgg cttcgatcat cggtgatctg
     2221 gaccagttgt tctgccaggg ctaaatcacc acgcgatact cctaatgcga gatcgataaa
     2281 ggtcggaagt aaattaggat ttttcgtcgc agccagtctc atttctgaaa aagcttcatc
     2341 gtaacgaccg atacgaagtt ttagattgcc tctcgcccag tttgtgtggg cgtaaaacgg
     2401 cgcaaagcga actgcctggt tgtaggcact caacgcgccg tcagagtcgt ctaaaaaatc
     2461 gcgaaagttg ccaagagtca accagattga gtcgtctcgt ggcctgagac tggcggcaat
     2521 ttccagttct ttcgtcccgt ccccagtcag tcgcaaatgg tcgagcacga ttgcccgagc
     2581 tcgatgagct tcggcgtcag ccggtgtaat ggccacggcc tgatttgccg caatcaatga
     2641 gttggacgat agggcgtatc gtgtaagcaa tctcgaaaag ccgtacttac ctgccgctag
     2701 gatcaagacc acagagatca atatgcccag gagcgaagtg actattcgag gtaatgcaga
     2761 gcgcaagaga tctctttcag cgtaacagaa aagcgtgctc aaccttaggg aagagcacgc
     2821 tccgaattat gggattgaaa accttagagg ccagcgccgc tcgggttgtc ctggaaagca
     2881 aaggggatga gggccgcgct accggctccg gcgagaatcg caactgttcc tgcagtacct
     2941 agcccgaaca atccgccggc gccgcctgca ccaccagccg ctgcaccgcc tgccgcaccc
     3001 gcacccgcac cagccgctac tcctggacca actgtgggag cagctgctcc tggtgggaag
     3061 caaatctcga gaacgccacc tttagctgga tccgttttgc ccgccgtgcc gccctgctcg
     3121 gttccgactt caccagtcgt cttcttctta gcagatagaa tggcgcatcc gaatagaacg
     3181 gtaaccttaa cgttgccatt ctcatcgtag gtcaatacga gcttggtatt gggagcgata
     3241 tcaagcgagc cgagagggcc aaggtttaca gtcgcggctt gatcagcacc tgtttcgatt
     3301 actgcaccgg tgactatgga tgcgccagtt gcggcaccca atccatttac ggtgataggc
     3361 tgattatttt tagtggtgag tttcgcgatg aattgctgtg gaaccaccac cggagagccg
     3421 ggagtaggtt cagcaaatcc cacctgaaca ccaacctgtg ctactgagaa caaaaccaac
     3481 gccgcaatac cctgataacc tctacgtcgt ttcaagctca tctaattttc ccttctcgga
     3541 gttacagatc gaggccgaac accaatttcg cgcgttcagt gtactggtgt tataccgtcg
     3601 ggtcaattgt ttttcctttt aaccaggagc tctaacagtc ttaattgcaa tccggtacaa
     3661 gctatttctt gaggtaacgc tggaaaaaat catcagctaa ttttggatcg atcgtcttga
     3721 ggcgattgta ttgatccagt gcgttcttcc ggtcattgag tgcgacgtaa gtcaaagcga
     3781 gattgaattg cgcttcgccg taatccggtt tgatcctgac cgcctctttg aacgaggcta
     3841 gtgcatcggg ataacgcttg gttcgaaagt acaccgtacc gacattgttg tttgtctcgg
     3901 cgctgccggg tttaagggcg agagctttct tgtaggcctc gatcgcagga tcaaattgct
     3961 gattcttgta aagagctgaa cctaaactat tgtacgcatc cgtatagtca gcttttaatc
     4021 ggactgcctg ctggagagcg tttactgctt cggcgaattg attccggtta tagaaagcgg
     4081 taccaagatt gtattgagcc tccgggaaat ctggtttcag ttgaacggcc cgttgcaggg
     4141 tcgtcactgc ctcttgatac tgtccgagtt tattgtaaac agctccgagg ttgtttagcg
     4201 cctttatgta gtcaggtctc agtctgatag cttctttata cgattctacc gcacgttgaa
     4261 agtacggaac tgctgctgtt cggtcgaccg tttcacctaa cttcacattg gcatacccca
     4321 gattgttatg tgatacagga ttgtcaggct taagtctgat agcttcattt agctccgtaa
     4381 ttgcttctcg gaattcgccg agcgcgatta acaagctgcc aaggttggtt cgtgcttcaa
     4441 cgtaattgct ccggagttgg atcgcacgcc gataagctgt ggtcgcttcc cggtaccttg
     4501 tggtcatgta gaaggcgttg cccagattgt agtttgcatc cggagaatca tttctgatcg
     4561 caacggcctt ttggaaattg gtcaaggcct ggtcgtattg ggcgactaca tagtgcgcag
     4621 cgccaaggtt attcaaggcg tcagcatcat ctggttttag ccttacagca gaactaaacg
     4681 catcaatagc ttcctgataa cgaccggctt tatagagact ggctcctaga ttgttgtacg
     4741 caacggcatc gtccgccttt agtttgactg agttgcgaag ggcttcgatt gattcattcg
     4801 agttccccgc ccggtccagt gcataaccta gattactgta ggccgtcgcg ttgttaggct
     4861 gaagtcgaac tgtctgccgg taagcagtgg ctgcctcaga aaactggccc agtgccaggt
     4921 aggtatcgcc aagcgcattc tgagcctcgg cccaatcagg cttaatccgg acggcttgtt
     4981 tgaaggcatt caaagcgtct tgctgcttgt ccataccact gtaggcgata cccaaattga
     5041 aataagcatc gggatcgtcg ggtttgattg atatagattt attgaaagcc tgggccgctt
     5101 cattgttctt gttcacactg aaataggcgg cgcccatgtt gttgtagaga actggatcct
     5161 gaggagtaag cttcaatgcg ttttgataag cagcaatagc gtcagcaaat ttccctacgt
     5221 cgtagtaagt attaccttga ttcttgaaag cttcggcttt gtcactatct gttggaccag
     5281 tctcagtctg gcccggacga actgtcggac gccttccagt gtttctcgct acttcgactt
     5341 gtcgagttgg ccttggctgt cgttttacga ccagcattgc cgcgccgccg accagatcac
     5401 gaacgcggtc cgggcgtttg aagataatgg ctgcaccgcc catgatgtcc tgtccctgaa
     5461 agcctccgct gccgatatcc tgaggtaaca acgggcggag catggcccat cgtgaagttg
     5521 aatatccccg gacgcgagga acaggcgtcg aaattaagag cgaagcggcg gcgaggcctg
     5581 ctaccagcga cgcttttgtt gtcttcttca atttgatgag ctttccaacc gaatctcagc
     5641 agttgacatc attaacaaca ccattagcac gagggcgttt actaaaatgt gcaaaccgaa
     5701 gtcgaataaa ctatgaaccg cgacgcccgc gatccccatg atcgcgccga cacacactgc
     5761 gcggaggaac ccgcttcgag tgttcaaggc aactttgatt ttccgaaagg cggtaatcgc
     5821 aaaccataat ccgagtgcga agccgacgac accaccgctt gaaagcagct ctaagtaatc
     5881 gttatgtgct tcctggggag tgaccattcc tgaagcatcg tggtaagccg tgataccaat
     5941 ccaataccca cccagtccta cacccagtat tggatttgca gtgaacatct tcagtgtggc
     6001 tcgccagatc tcgtttctac ttgcgccgct ataggcagat tgagtttctg gattcaattc
     6061 gttcgaggca gactgaaagc tttttaccag aggatcgcca ccaacccata tggtaccgcc
     6121 gaccagtccg aatacgaggg caaccaccaa tacggttttg aatagccgcg aactctggat
     6181 ccgggttaat ccgttttggt ccacgtgcga taaccttgaa cctctgacga gaataagagc
     6241 ggccaggatg acctgtgtca gcattgcgag tattcctccc cgtgaattgg agaggaccag
     6301 tgccatccaa atcggcagaa gcaacgcgac attaagcaac acccgatctc tctttactcc
     6361 accggccaac gctatcccga gtcctaaacc gaaggccatc tccatcaaat atgcgaaatg
     6421 gtttctattg atgaactgac catatccctg gtcgggttca agaaatggca atatgaaacc
     6481 agtaccgtgt tgcgttgttt gtcgcacgag tccaaagatt gcactcagcg ttgctactga
     6541 caatgtgaca tagactaacc atctgatccg agaatcagtc gcagcataac ggtatagcaa
     6601 tgctagaacg gtcgcgagag ccagaagttg caacgcaaag aatcgcgttt gatagggatc
     6661 ggcactgata gctgtaaatg aaggggagct ggttaagccc tcgataggtg tggcccgtct
     6721 gagtgggaga gtttgtaaga atgcaaaaat cgacaggcaa atcaatggga cgaccagaaa
     6781 cattccttta gatttcgctg agtcgttcaa ggcatgctcg attaacgcaa caatacagat
     6841 cgagaagact ccgcaaacaa aaagagcttt ccaccacggt tgtgcggtac cgtaaggcaa
     6901 tgctgcgatt acaatgagtg cgagtaatgc gccaaagatt agtttcgaca acaggtcagc
     6961 tatcctgctc gctgacgatt gctccgcttc agcgaacgtt gagacgactg ccacatcctc
     7021 cattatccgg ggatctcctt cggcgctgca tcgataggga taatggcagt ctgtgacgcc
     7081 ggttcatgac cgctgataat ccgtgcggct
//
LOCUS       DASSFP010000223         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2141167, whole genome shotgun sequence.
ACCESSION   DASSFP010000223 DASSFP010000000
VERSION     DASSFP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141167"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFH91_04835"
     CDS             <1..705
                     /locus_tag="VFH91_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HET6862344.1"
                     /translation="ENTDHHLIVGKGAQEFARNMGFKIEDDLNTDHSRKQWLEWKRRT
                     DPSHYLDPKKRAEAGHKAALEMLAEGFLREENLHGTINCDGINSKGEICGVTTTSGLA
                     WKIPGRLGDSPILGAGLYVDGEVGAAGSTGRGEANLYNLCSFVIIEEMRRGAHPKDAG
                     MEVLKRIKNNTVEKRLLNSRGLPNFGINFYILNAKGEFAGVTMYEGSSFAVCNENGPQ
                     TLKADALLQGKAGETD"
     gene            complement(792..1142)
                     /locus_tag="VFH91_04840"
     CDS             complement(792..1142)
                     /locus_tag="VFH91_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862345.1"
                     /translation="MSKPVIGIVLGAILGAIDGLTAWFTPAVRDQLAGIVIGSTIKGI
                     IAGVAIGFFAKKYHSLPIGILFGVIVGAILAFLVALMQGGYYFEIVLPGTMVGLIVGF
                     ATQKYGKTTATANS"
     gene            complement(1200..2225)
                     /locus_tag="VFH91_04845"
     CDS             complement(1200..2225)
                     /locus_tag="VFH91_04845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6862346.1"
                     /translation="MKWLSDQKLNHLRRVVSEPDFSGTKYSIVKELGSGGMGSVYLAH
                     DSELDREVAIKVLRETDLSDDLKRRMLREAQIIARLEHPGIVPVHDVGVLEDGKVYYA
                     MKFVRGSRLDEYAATITTPGDRLRKFKSVCETVAFAHAHDVVHRDLKPQNIMIGSFDE
                     VLVLDWGIAKVLRKTSTPDPEAETLKLPSVESTTSHDTTDGAIIGTRHYMSPEQARGD
                     QVDSRTDVYSLGAVLYFLLTNIAPAASTDHSCFVLPRTVDPAISKAAEAVCLKAMADD
                     RQRRYASAAELSADVGRLLDAEPVSAYRENILEKTGRWLGKNRFLVLLVLAYLIMRIF
                     FIFTARD"
     gene            complement(2222..2977)
                     /locus_tag="VFH91_04850"
     CDS             complement(2222..2977)
                     /locus_tag="VFH91_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6862347.1"
                     /translation="MDPDTDIGGPNHRFPATNESAIVRIRSGDEQTRERAFESILTSY
                     WKPAYKYIRWKWHASNEDAKDLTQSFFAVAFEKNYFSAYDPAKASFRTFIRTCLDGFV
                     SNQQKSDKRLKRGGLSEHLVLDFMSAEAELALQYTAQDLTPEDFFHREWVRSFFTVAV
                     ERLRERLSSSNRHLHLKLFEIYDLNDDEPDERVSYAKLAQQFNLDAATVNNYLAAARR
                     EFRKIVLEKLAEITATEEEFRSEARSLLGLELK"
     gene            3047..3445
                     /locus_tag="VFH91_04855"
     CDS             3047..3445
                     /locus_tag="VFH91_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862348.1"
                     /translation="MKRFHLIFGLSLFVVFLLTGQFMDRFHHHLADMPDGPRMLYRSR
                     HIYILLSGLVHLGIGSYFYYRLQTWLRVMQLLGSALITIASVMFVVAFFYEPGLKNLY
                     GPLTKKAVIITAVGMLLHLLGSREAVKHER"
     gene            3464..3844
                     /locus_tag="VFH91_04860"
     CDS             3464..3844
                     /locus_tag="VFH91_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862349.1"
                     /translation="MKLITRMVMVAVLLSTAVTGHSQNAAADPAFNTFWKTFKAAIAK
                     NDKEAVADLTKLPFLYESKERDRAGFIRIYPSLFTPRIRKCMATAKLVKEQENYDVFC
                     GQLIFYFGKDSDGKYKFLEFGVND"
BASE COUNT         1017 a         1007 c          962 g         1007 t
ORIGIN      
        1 gaaaatacag accaccatct gatcgtcggc aaaggcgcac aggaatttgc gcgcaacatg
       61 ggtttcaaaa tcgaggacga tttgaacact gaccattcga gaaagcagtg gctcgaatgg
      121 aaacgacgca ctgatccatc acattacctc gatcctaaaa aacgcgctga ggcaggtcac
      181 aaagccgctc tcgaaatgtt ggccgagggc tttttgaggg aagaaaatct acacggcacg
      241 atcaactgcg atggaatcaa ttcgaagggc gagatttgtg gtgttaccac aaccagcgga
      301 cttgcctgga agattcccgg caggttgggc gactcgccta ttcttggcgc cggattatat
      361 gtcgatggag aggttggagc cgctggctcg acaggtcgtg gcgaagcaaa cctctataat
      421 ctttgttctt ttgtgatcat cgaagaaatg cggcgaggcg ctcacccgaa agatgctgga
      481 atggaagttc taaagagaat aaagaacaat acagtagaga aacgattgct caatagtcgt
      541 ggcttgccga attttggtat caacttctac atcctgaacg caaaaggtga gtttgccggc
      601 gtcaccatgt atgagggttc ttcatttgca gtttgcaacg agaatggccc gcaaactctg
      661 aaagcggacg ccttgttgca aggcaaagct ggagaaaccg actaacggta tcgttctgaa
      721 gattatgacc tcacagactt gaaaccatca cgcttggcaa aagcgactat gctttaacgc
      781 ccaatcaact tttagctgtt tgcggtagca gttgttttgc cgtacttctg ggttgcaaac
      841 ccaacaatta gaccaaccat cgttcccggc aagactatct cgaagtaata gccgccctgc
      901 attagggcca ccagaaacgc cagaatggct cctacgatca cgccaaacaa aattccaatt
      961 ggcagcgaat ggtatttctt cgcaaagaag ccgatggcga ctccggcgat aatgcccttg
     1021 attgttgaac caatgacgat gccggccaac tgatcgcgga ccgccggcgt gaaccacgca
     1081 gttagaccat caatcgcacc gaggatcgct cctaaaacga ttccaataac tggtttggac
     1141 atactcttct cctaagttta ctagtcttat ctacctatta aacagttcca cgagtttctt
     1201 taatctcgcg ctgtaaaaat gaagaaaatt ctcattatca gataagccaa taccagcagc
     1261 accagaaagc ggttcttacc gagccatcgc cccgtctttt caagaatatt ttcgcgatac
     1321 gccgaaaccg gttcggcatc aagcaggcgg cccacatcgg ctgacaattc agcggcgctg
     1381 gcatagcggc gttggcggtc gtcggccatc gctttcagac aaacagcttc agcggctttg
     1441 ctaatcgcag gatcgaccgt tcgaggcagg acaaaacaag aatgatcggt actcgcagcc
     1501 ggcgcaatat tcgtcaagag aaagtacagt actgctccga gggaatatac gtccgtgcga
     1561 gaatcaacct ggtctccgcg agcttgttct ggcgacatgt agtggcgcgt accgattatg
     1621 gcgccgtcag tggtgtcatg ggacgtagtt gattctactg atggcaactt gagtgtctca
     1681 gcctcaggat cgggtgttga cgttttgcgc aggaccttcg cgatccccca gtcgagcacc
     1741 agcacttcgt cgaaggagcc aatcattatg ttctgtggct tcaaatctcg atggaccacg
     1801 tcgtgagcgt gagcaaaggc aacagtttca cacaccgatt tgaatttgcg caggcgatca
     1861 cccggtgtcg ttatagtcgc ggcatattcg tccaaccgac taccgcgaac aaacttcatg
     1921 gcgtagtaga cttttccgtc ttcaagcacg ccaacgtcgt gtaccggcac gattcccgga
     1981 tgttccaagc gtgcaattat ttgagcctct cgtaacatgc gacgcttcaa gtcgtcactt
     2041 aaatcggttt cgcggagaac tttgatcgct acctcgcggt cgagttcact atcatgggcc
     2101 aaatagaccg agcccattcc tccgctccca agttctttaa cgattgagta tttagtacca
     2161 ctgaaatcag gttcgctgac aactctgcgg aggtgattca atttttggtc ggacagccat
     2221 ttcatttcag ttccagcccc aggagcgaac gcgcctcgct ccgaaactct tcctccgtag
     2281 ctgtgatctc tgcgagtttc tccagcacaa tctttcggaa ttcacgtcgt gcggcagcaa
     2341 ggtaattgtt gaccgttgcg gcatcgaggt tgaactgctg cgctagtttc gcgtaagaca
     2401 cacgttcatc cggctcgtcg tcattcaggt cataaatctc aaacagtttt agatgtagat
     2461 gacggtttga tgatgacagc cgctcgcgga gcctttcgac cgcaactgtg aagaagctgc
     2521 gaacccattc gcggtgaaag aaatcttcgg gagtaaggtc ttgagcagta tactgaagag
     2581 ccagttcagc ctctgcgctc ataaaatcga gaacaaggtg ttcgcttaga ccgcctcgtt
     2641 tgagtcgttt gtcggatttc tgttgatttg aaacaaatcc atccagacag gttctgataa
     2701 aggttcggaa acttgctttg gcgggatcat aagctgagaa ataattcttt tcgaaagcga
     2761 cggcaaaaaa actctgagtc agatctttcg cgtcctcgtt gcttgcatgc cacttccaac
     2821 ggatgtattt gtaagcaggt ttccaatagc tggtcaggat cgactcaaat gcccgctcgc
     2881 gcgtttgctc gtcgccactc ctgatacgta cgatcgccga ctcgtttgtc gccgggaacc
     2941 ggtggttagg accaccaatg tcagtatcag gatccataag actcagacca tttcaatagc
     3001 ctttggcgag atcaagactt tcgctttagt tctggaagaa tctgcgatga aacgatttca
     3061 ccttatcttt ggtttaagtc tattcgtcgt cttcctctta acaggacaat ttatggaccg
     3121 ctttcatcac catcttgccg acatgccaga cgggccacgc atgttatatc gatcccggca
     3181 catctacatt cttctgtcag gtttggtcca tctgggcatc ggctcctatt tttactatcg
     3241 cttacaaacg tggttacgag tcatgcagtt actgggctct gccttgatta caattgcttc
     3301 ggtcatgttc gtggtagcgt ttttctacga gcctggcctg aaaaatctat acggtcctct
     3361 aacgaagaaa gccgtaatca tcaccgcggt gggaatgttg ttacacctgc tcggcagtag
     3421 agaagcagtg aaacacgaac gctaacttca aagggcacat cctatgaaac tgattactcg
     3481 aatggtaatg gtggctgtac ttctgagtac agccgtaacg gggcactcac agaacgcagc
     3541 agctgatccg gctttcaaca ctttctggaa aacattcaaa gccgccatcg ctaagaacga
     3601 caaagaagcc gtggcggacc tcacgaaatt gccttttctc tacgaaagca aagagcgaga
     3661 ccgggcaggt tttattagaa tctatccttc tttatttaca cccaggatcc gcaagtgtat
     3721 ggccacagcc aaacttgtga aggagcagga aaactacgat gtgttctgcg gccagttgat
     3781 cttttacttc gggaaagatt cagacggcaa atataagttt ttagaattcg gagtaaacga
     3841 ctgaactgct cgtcaggatg ttgctacttt tgcttccgct tgccgtttca caatcactag
     3901 agtgatatcg tcggcagcac tcgtgccttg cgcaaattta gtcaacgccg actcaatccg
     3961 atctcttatg cccgctgctg agctgttaac gtt
//
LOCUS       DASSFP010000224         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1997628, whole genome shotgun sequence.
ACCESSION   DASSFP010000224 DASSFP010000000
VERSION     DASSFP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997628"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFH91_04865"
     CDS             complement(<1..618)
                     /locus_tag="VFH91_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862350.1"
                     /translation="MNNRKRVAVSSNTAARLQAAARWIESQLNSSELLIISHSIDAAS
                     NFCLSVASGREALFGTRRLTLNGLAARLAQRSLAQSETVPATALSFNGIVARAIYLLN
                     AQDKLTYFAPVATKPGFPIAVARTLEELRMNDVRPDTLASLPGGGKDLGEIARVVEAE
                     LDDSKIGDRAKIFQAAIASISLLHNSDYVRVPTLLLDVCIRSQLEF"
     gene            complement(695..1906)
                     /locus_tag="VFH91_04870"
     CDS             complement(695..1906)
                     /locus_tag="VFH91_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862351.1"
                     /translation="MIKLRVRSTGTFIILLPLVLTLGCKSVINRQDVRPRVLRDVPAQ
                     NLAYRLDADVSPPSGSKIDDTIDKQLAVQSFFTSKREDDALLRTVTSPDGRRVLALYG
                     TADEPSSAFRIDLFNSDGQFLRNLIPPELSCVFPETVAWSPDGNSITFVAHRRVAPSP
                     SPTPPIVEPDPEAPPAPAPSVAPVFAPVATFNTEQIYICDRDGYNLRPLTSREGLIYF
                     YFAWAPDAHALVALACKEDEWNAREKQYKLPAGRPRLIGLDGQERLLDDQLTEAQPVW
                     SPDASKVATAFESVVTIYDAVTAKPTQARLPLTDALLAASVAYEQKTGNTTSSTPKGG
                     TPGPSPRAEQTSNQSAVPASFNPIVRLEWPSPERLYFQTAFVRLMPNEAINTFQRWHL
                     LSLSAQAAILK"
     gene            complement(2000..2914)
                     /locus_tag="VFH91_04875"
     CDS             complement(2000..2914)
                     /locus_tag="VFH91_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862352.1"
                     /translation="MSIPKALLCLVLTASLVNSMSAQAQQRKIVKSGSHPVRTRASMS
                     PAERALIDEAILVVCSQAKLDPNGSLAIDEMQARPSLPVHSPEALAGAARAQRLLPVA
                     KTLVISSLTQLMKDYGLTKSGTDSSRLQQSTARVQAVIKVKPDMEARDNASVYLNRPR
                     TITFGTLFLAGLRSDEGMVSVLAHELVHIADGPNDNLRVLINAIADKASSLTGLEIHG
                     QRAEELTCDLIGAMAVRTFVGDSPGYESVARRLARSVEHNCVTQDEGDEEHLSPRSTI
                     RALLSLNPQLVRELVNDQEEVSPKRTKN"
     gene            complement(2935..3708)
                     /locus_tag="VFH91_04880"
     CDS             complement(2935..3708)
                     /locus_tag="VFH91_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6862353.1"
                     /translation="MATYQNIKLNVEDRVARISLARPPLNVFNIAMMRELAAAVQEMV
                     NQEIVAIVFEADKETRAFSAGVAVEEHAPETVYQMLDSFHSVFRALEQVAKPSIAVVD
                     GVAVGGGCELVSACDIVIASDRARFGQPEIKLGVFPPVASIFLPLVIGEKRARELILT
                     GDIIDANEAGRLGLCTHVVPSNHLDQKLTEVVSKLRELSSVSLEYARRALDLARPRKL
                     DQTLKELENLYLHELMKSYDAIEGVKAFMEKRKPAWRNR"
     gene            complement(3725..>4276)
                     /locus_tag="VFH91_04885"
     CDS             complement(3725..>4276)
                     /locus_tag="VFH91_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6862354.1"
                     /translation="YLYNEFYKKDRNYSPEDFQKACEAMAGTSLDSFFSKYVRAKDEL
                     DFNASLSTAGLKLETTATGEGGKPIERVFLGADTQEVDGKLIVRRVIAGSPAYDQGLN
                     SGDQIVALNNMRTDKQFFDARIAEKKPGDLINLTIFRADDLSTLLIKLGGRSDISYRI
                     VPLPNVTPAQKEVYRAWLGVGTN"
BASE COUNT         1038 a         1075 c         1097 g         1066 t
ORIGIN      
        1 aaactccagc tgactccgga tgcagacatc caaaagtaat gttggtactc gaacgtaatc
       61 ggagttatga agtaaggaaa tggaagcgat ggccgcctga aagattttag cgcggtcacc
      121 gatctttgaa tcatctaatt ccgcttctac aacgcgggca atctcgccaa ggtccttacc
      181 acctccgggc aaggacgcga gcgtatccgg gcgtacatca ttcatcctca actcttcgag
      241 agttcgtgca accgcaattg gaaagccagg cttagtcgct accggagcaa aataggtgag
      301 cttatcctgc gcattgagaa gatagattgc tctggcaacg attccattga atgacagcgc
      361 cgtggcggga accgtttcgg actgtgccag ggagcgttgt gccagtcgcg ccgcaagacc
      421 attcaaagtc aaccgccgtg ttccaaacag agcctcacga ccgctggcta cggataaaca
      481 aaagttgctg gcagcgtcaa ttgagtgact gatgatcagc agttccgaac tatttaattg
      541 agactcaatc caccgcgcgg cagcttgaag acgtgctgcc gtgtttgagg aaactgctac
      601 tcttttgcga ttgttcattt actacccaag cattatgtaa gggaagccag gagtatacca
      661 acgcagtgtt cttaaaacgg aatgtttagt ggatttattt taggatggcg gcctgcgcac
      721 tcaagctcag aaggtgccag cgctgaaaag tgttgattgc ttcgttgggc atcaggcgga
      781 caaatgcagt ctgaaaataa agtcgttcag gagagggcca ttccaacctg acaattgggt
      841 tgaatgaggc cggaacagcc gattgattcg atgtttgttc agcccttgga ctcggaccag
      901 gagtgccacc ctttggagtc gaactcgtgg tgtttccggt cttttgctcg tacgcgactg
      961 aggcggcgag taaagcgtcg gtcaatggta accttgcctg ggttggcttt gcagtcaccg
     1021 catcataaat tgtgaccaca gattcaaaag cggtcgccac ttttgaagcg tcgggcgacc
     1081 aaaccggctg agcctcagtg agttgatcgt caagcaatcg ctcctgtccg tcgagaccga
     1141 taagccgtgg gcgaccggca ggcaatttat actgtttctc gcgagcattc cattcgtctt
     1201 ctttacaagc taaggctacg agcgcatgcg cgtcgggagc ccatgcaaaa tagaagtaaa
     1261 tcaatccttc ccttgatgtt aatggcctga ggttatagcc atcgcgatcg caaatgtaga
     1321 tctgttccgt attaaacgtc gcgacaggcg cgaatacagg tgctactgaa ggagcaggag
     1381 cgggcggagc ttccggatca ggctcgacga tcggaggagt cgggctagga ctgggagcca
     1441 cgcgacgatg agctacaaag gtaattgaat ttccatcggg cgaccatgca acagtttccg
     1501 gaaacacaca cgagagttct ggcggtatca agtttcgcaa aaactgtcca tcggagttga
     1561 atagatcgat cctaaacgcc gagctaggtt cgtcggcggt accataaagc gccaggaccc
     1621 gacgaccgtc gggcgaagta actgttcgca gcaatgcgtc atcttcacgc ttcgaggtga
     1681 agaaactctg cactgcaagt tgcttatcta tggtgtcgtc gatttttgat ccggagggcg
     1741 gcgaaacatc cgcatccagt ctgtaggcaa ggttttgtgc cggaacatcg cgcagcactc
     1801 gtggtctgac gtcctgtcga ttgatgactg atttacaccc gagcgtgaga actagaggca
     1861 gtaggatgat aaaagttccg gtgctgcgga ctctaagctt aatcattggt cagtggtcat
     1921 ttgatatttg agaattgtta atcgattcga tccggagcaa atactaatta tcaaatctca
     1981 aatgataatt gagcattgtt cagtttttgg tacgctttgg agaaacctcc tcttgatcat
     2041 ttacgagttc ccgcaccaac tgaggattga gtgacaagag agctcgaatc gtgctgcgag
     2101 gtgaaaggtg ttcctcatca ccctcatctt gcgtcacgca attgtgctcg actgaacggg
     2161 cgagcctgcg cgctactgat tcataacctg gagagtctcc gacgaaggta cggacagcca
     2221 ttgctccaat tagatcgcaa gtgagctctt ctgccctttg tccgtgtatc tcaagtccgg
     2281 tcagactcga cgctttatcg gcgatcgcgt ttatcagcac tcttaagttg tcgtttggac
     2341 catcagcaat atgcactaac tcatgagcta acacactaac cattccctcg tctgaacgaa
     2401 gacccgccag aaaaagagta ccaaaagtga tcgtacgtgg acgattaaga taaacggacg
     2461 cgttatcgcg cgcctccata tccggtttga ccttgataac agcctgcacg cgcgccgtcg
     2521 attgttgcag tcgcgagcta tccgtccctg atttagtcag cccgtaatct ttcatgagtt
     2581 gggtgagtga tgaaattaca agagtcttgg cgacgggtaa taatctttgt gcccgggctg
     2641 cacctgcaag agcctcgggg ctatgaactg gaagcgaagg tctcgcctgc atctcgtcga
     2701 tcgcgaggct gccgtttgga tccagtttgg cctggctaca gacaaccagt attgcttcgt
     2761 caatgagcgc tctttccgcc ggcgacatgg acgctctcgt gcgaaccgga tgcgagccgc
     2821 tttttacgat cttccgctgt tgcgcttgag ccgacatcga gttgaccagt gatgcggtca
     2881 ggacaagaca cagaagagct tttggtatcg acataattac aactttctta atcgctaccg
     2941 attacgccag gctggttttc gtttctccat gaaagcttta acgccttcaa tagcgtcgta
     3001 gctcttcatc aattcgtgca agtagagatt ctcgagttct tttaaggttt gatcaagttt
     3061 tcgcggtcgg gctaaatcga gcgcacgcct cgcgtattcc agcgatacgc tggagagctc
     3121 acgtagcttc gagacgacct cggtcaactt ttgatccagg tggttcgagg gaacaacatg
     3181 agtgcaaaga ccgaggcgac ctgcttcatt agcgtcgatg atgtctccag tcaaaataag
     3241 ttcgcgtgcg cgcttttctc cgatgacgag tggcagaaag atggatgcaa ctggcggaaa
     3301 aaccccaagt ttaatttctg gttgtccaaa acgagcgcgg tcacttgcga ttacaatatc
     3361 gcatgctgaa acgagttcgc acccaccacc aaccgccaca ccatcaacca cagcgatact
     3421 tggtttcgcc acctgttcaa gtgcgcgaaa gaccgagtgg aacgaatcga gcatttgata
     3481 aaccgtttct ggagcgtgct cttcgacggc tacgccggcg ctgaaagccc gcgtctcttt
     3541 gtcggcctca aatacgatag ccactatttc ctgattcacc atctcctgaa cggctgcagc
     3601 caactctctc atcattgcga tgttgaatac attgagtggc ggtcgggcca gcgagatgcg
     3661 agcaacgcga tcttcgacgt ttagtttgat gttttgataa gttgccattt gtcgtgtatt
     3721 cgagctagtt tgttcccaca cctaaccatg ctcggtagac ttccttctgc gctggagtta
     3781 cgttgggcaa cggcacgatc cggtaagaaa tgtcggaacg accgccgagc ttaatgagaa
     3841 gcgtgctcaa atcatcagcg cgaaagatgg tcaggtttat caagtcgcct ggttttttct
     3901 cagctatacg agcatcaaaa aactgtttat cagtgcgcat gttgttcagg gctacgattt
     3961 gatctccgga atttagcccc tggtcgtacg ccggcgatcc tgctatgacc cgacgcacaa
     4021 tcaatttgcc atctacttcc tgcgtgtccg caccgaggaa aacacgttcg atgggctttc
     4081 ctccttcgcc cgttgcggtt gtctccaatt taagaccggc ggtcgaaagc gaagcattga
     4141 aatccagttc atcctttgca cgaacatatt tcgagaagaa actatccagg ctcgttcccg
     4201 ccatggcttc acaagccttc tgaaaatctt caggactata attccggtcc ttcttataaa
     4261 actcgttgta gagata
//
LOCUS       DASSFP010000225         5678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2141469, whole genome shotgun sequence.
ACCESSION   DASSFP010000225 DASSFP010000000
VERSION     DASSFP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141469"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..2026)
                     /locus_tag="VFH91_04890"
     CDS             complement(167..2026)
                     /locus_tag="VFH91_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HET6862355.1"
                     /translation="MNELGGIVNFDDAAVDPDELSSLERSLSAYGCDESFSLLTASAG
                     ICFCAYHTTTKSQLTKQPLSFENGSIVVFDGVVQNRNELLRSFKLSVETSDVEIVAAG
                     LARYGNPFIKQLIGSFALAWWNQIDRRLVLARDHIGSKPLYYRIRKQSIYFASEVSVL
                     LASGTCTDLDEEYIAGFLGAGPKTDLTAYQSVKAVKPAHVLEINRKGIESHQFWSLSN
                     VIHLNYSTDEAYEEHFNSVAREAVSNVIERANGPVFSELSGGLDSTAIVCLADEVTTK
                     LGRPAIETLSRVHDDSPSSDEREFIQLVEKQRRKPSLYIREEDFPLLSGANSKFSVNG
                     PNPFYCFSEYHQAVCQILRRKGVRTLLSGKGGDQILGGNPSGVPELADLFVQRRFFAF
                     ARSLRTWSLANRGPSFPLVGQSIRAALQARAPRFIRSANWSPPNWIASSFRKRMYLGP
                     FTESDHSCGLPSDLDQSAGFSSVVATVSAQYRRHWGKIDVMHPWLYRPLVEFMQAIPF
                     NQRVRPGENRSLMRRSLQEVLPARIVHRRGKRSPGEALVRAIRREFSRLQSLFDQPLI
                     AAYGYADADELQSTLVRAREGSDLTSLSIVLAVSLEVWLRTINDIKNGFPEGT"
     gene            complement(2052..2831)
                     /locus_tag="VFH91_04895"
     CDS             complement(2052..2831)
                     /locus_tag="VFH91_04895"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HET6862356.1"
                     /translation="MPVTSPKAQLESDGVVLYSREAERLYRLNSTAAYVWSLLETQPG
                     ALTIQQLHARLRSEANRLNLPDSESLSPLQTDKLIRQLTALGLVTVKKWPDGECRYVP
                     LAVRPKENYAGTAVTDKGWPNVMAERNVALLTRVKIHTVAGVYLLFAAIDLYLKFAGF
                     SALLSRVERYPTNSKCRHSDVVTNIAALDHAQVYYPKKQMCLQRSAALTLLLRRHGHF
                     AQMVIGTQTFPPKAHAWVEIDDQVIGDSPKIKTLYQELLRV"
     gene            complement(2865..>5678)
                     /locus_tag="VFH91_04900"
     CDS             complement(2865..>5678)
                     /locus_tag="VFH91_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HET6862357.1"
                     /translation="THSQSATDGERIDSSKDLVTALINAGNNPDRVNYLLENNSPFIS
                     PNLWQSLMDAAAHAYHNRQPDQGIETYRLACEVALRLHDQNLIGKTFHNLGRSYSGLN
                     QFDKAKEAFLSSRKAFSAADSVHDLLYIFSDLGTVSLITQDYEAARNYSEECIKLWRA
                     LGSPDSTTNELDTYGAAGAFSTLGELGTREGNLWDAFNNLQRSLELFDELERRHPTYG
                     YYVADVCASIGRLYTSTGDHVRGLAFLNRALALSTTLNQSNQEAALLNDIGYLYVEQE
                     DYSQAWAKFHDSLQRYRAGKNRREESRSLLNLGVVQQRQKRFEEALTLFQTSLELAKA
                     TDNFEVQIAAGEGIGAVFAAKRDFQSALQIFNSSLLLALERKERIREAELLWRIAEVR
                     FAMGDFDSSLGHAEAALEIARLMHSPKLTYLALAAIGQTYAAMSRPEVAIETLKQAID
                     TVELMRHKVVGEEIERQLFFQDKVSSYRMLIDLLLKQNNFSEALLFAERAKGRVLLDV
                     FRSVSDPFPSTVERSEVTRRKRNASDFNEAQSSDQEQLNRTIREDQTFRNSLYALHPD
                     LKVRLGRTAEITASDLARFTVQPDTAYLEYVIGKDGIDLFLLRAGPQETLLKSFRIEV
                     RPETLRKQINTLNSRLAIRHPDFESFGRELYRRLVQPAQGELAGIKKLCIIPDDFLWN
                     LPFQALTTNHNKYLLEEYSLSYAPSLSVLIEMKHKVRSNPKAALAAFGNPKLTKEQRV
                     DDQIGPIPETESEVLSISKLFTSSRNKVLIGEAASEQSFNKLAKSFSTFHFATHGLLD
                     NKQPLYSHLLLTRTSDDGANDGRLEAREILNLNLTADLAVLSACETANGKISPGEGII
                     GMSWAFFVAGVRSMVASQWRVNSASTTQLMTDFYSGLRRKTHDPNQSRSQALRTASLK
                     LMRSSRYRHPFYWASFILIGADN"
BASE COUNT         1370 a         1371 c         1466 g         1471 t
ORIGIN      
        1 aggattacat cttcagcttt gccgatctct gaaatctcag gtgattccaa tacgcgtttc
       61 atgggattgt ctcctttctt gttgttctag attggcttgc gcctctctgc cttttccgga
      121 tcagttgatc gcggatggtt agtccagcaa cgttgtggaa acttggtcag gttccctcag
      181 ggaagccgtt cttgatgtcg ttgatcgttc gcagccaaac ctctaaagag actgccagga
      241 cgatcgatag ggatgtgaga tcagatccct ctcttgcacg aacgagagtg gattgaagtt
      301 catcggcatc tgcatagcca taagcggcaa tcagtggttg gtcaaagagc gactgcagtc
      361 gtgaaaactc acgtcgtata gctcgaacaa gtgcttcgcc cgggcttcgt tttcctcgtc
      421 gatgaacaat tcgcgccggc aacacttcct gaagagagcg acgcatcagc gacctgtttt
      481 cgcctggtct gactctttga ttaaagggaa tagcttgcat gaactcgaca agaggtcgat
      541 acaaccaggg atgcatgaca tcgatttttc cccagtgacg ccggtattga gcggagacgg
      601 tagcgacgac tgaagaaaat ccagcggact ggtccaggtc actcggcaat ccacaactgt
      661 ggtcggattc agtgaacggg ccgagataca ttcgctttcg gaaggaactg gcgatccagt
      721 ttggaggact ccagttggcg ctacgaatga atcgaggcgc acgcgcttgc agagcggctc
      781 taatactttg tccgaccagc gggaaagacg gtccacgatt agcaagactc caggttcgta
      841 aagaccgtgc gaatgcaaag aaccggcgtt gtacaaacaa gtcagccagt tctggaacac
      901 cgcttggatt tcctcccaat atttggtcac cgccctttcc cgaaagaagc gtgcgaactc
      961 ctttgcgccg caatatctgg caaacagcct gatggtattc gctgaaacaa tagaaaggat
     1021 tgggaccatt aacgctgaac ttactgttcg ctcctgagag gagcgggaaa tcttcctctc
     1081 gaatatagag gctgggcttt cgacgttgct tctcaactaa ctgaataaac tcccgttcat
     1141 ccgatgaggg cgaatcgtca tggacacgtg acagagtttc aatggccgga cgacccagtt
     1201 ttgtggtgac ttcatctgcc agacaaacaa ttgcggttga atcaagacca ccactgagtt
     1261 cactaaagac tgggccattg gctcgttcta ttacattaga aacagcctca cgcgcgactg
     1321 aattgaaatg ctcctcatag gcttcgtcag tcgagtaatt gagatgaatg acgttggata
     1381 gactccagaa ttgatgactt tcaattcctt tgcggttgat ctcgagaaca tgtgctggct
     1441 ttacagcttt aaccgattgg taagcagtaa gatcggtttt aggtccagcc ccgagaaatc
     1501 cggcgatata ttcttcatcc agatctgtgc aagtcccact cgcgagtaag accgaaactt
     1561 cagatgcaaa gtaaatgctt tgctttctta tgcggtaata gagcggttta gatcctatgt
     1621 ggtctcgcgc caataccaga cgacgatcaa tctggttcca ccatgccaga gcaaaactgc
     1681 cgatgagctg ttttatgaat ggattgccgt aacgtgcgag accagccgca acaatctcga
     1741 cgtccgaagt ttccacggaa agtttgaatg acctcaatag ttcgttccga ttttgaacca
     1801 caccgtcaaa cacgactatg ctgccgttct cgaatgagag tggttgttta gtcaattgcg
     1861 attttgtagt cgtgtggtaa gcgcaaaaac agatgccagc actggccgtc aagagggaga
     1921 aactctcatc gcagccgtag gccgataagg accgttccag actgcttagc tcgtccggat
     1981 ctacagccgc atcgtcaaag ttaactatac cgcccagttc attcatgtga ttaagtcgta
     2041 atgatgcgag ttcagactct cagtaattcc tggtagagag tcttaatctt cggactatct
     2101 ccgatcacct gatcgtcgat ttcaacccag gcgtgtgctt tcgggggaaa tgtttgagtg
     2161 ccgataacca tttgtgcaaa gtgaccgtgt cttctcagca gtaaagtaag cgcagcggac
     2221 cgctgcaagc acatctgttt cttcgggtaa taaacttgag cgtggtccag tgccgcaata
     2281 ttcgtaacaa catccgagtg ccggcatttt gaattcgtcg gatacctctc gacacgggac
     2341 aacaaagcgc tgaagcccgc gaatttcaaa tagagatcaa ttgccgcaaa aagaagatag
     2401 acgccggcaa ctgtgtgtat ttttactctg gtaagcaacg caacgtttcg ctcagccatg
     2461 acgttgggcc aacccttatc cgtaaccgct gtgccagcat agttttcctt tggtctaacc
     2521 gccaatggta cgtaccggca ctcaccgtca ggccactttt taactgtgac cagcccgagc
     2581 gccgtcaatt gccggataag tttgtccgtt tgcagcgggg atagggactc agaatctggt
     2641 aggtttaatc tgttggcttc cgatcgcagc cgggcgtgga gttgctgaat agtcagcgca
     2701 ccgggttgag tctccaggag agaccaaacg taagctgcag tggaattcag cctgtagagt
     2761 ctttcggctt cgcgggaata caagaccact ccgtcgctct cgagttgtgc tttgggtgaa
     2821 gtcaccggca tagagtgtga tccttgagac tgagttcacg ttaactaatt atctgcacca
     2881 atcagaatga aagatgccca gtaaaacggg tgtcgatacc tgctgcttct cattaatttt
     2941 aaagaggctg ttcgcaacgc ttgagatcgc gactgatttg gatcgtgagt cttgcgccgc
     3001 agaccagaat agaagtcggt cattagttgg gttgtactgg ctgagttaac ccgccactgg
     3061 ctggccacca tagatctaac gccggccacg aagaacgccc acgacatacc aattattcct
     3121 tcgccgggac tgatctttcc gttggctgtc tcacacgctg agagcacagc aaggtccgca
     3181 gtgagattta aattgaggat ttcccgagct tctaaacgac cgtcgttggc accatcatcg
     3241 ctggtccgag tcaatagtag gtgcgagtag agtggttgtt tgttgtcaag caggccgtga
     3301 gttgcaaaat gaaacgttga aaaactttta gctaacttgt tgaaggactg ttcactcgct
     3361 gcttctccaa tcaggacttt atttctactc gaagtgaaga gtttggaaat tgagagtact
     3421 tctgactctg tctcggggat aggtccgatc tgatcgtcga cgcgttgttc ttttgtaagt
     3481 tttggattgc cgaatgcggc caaagcagcc ttcgggttcg atctaacttt gtgcttcatc
     3541 tcgatgagaa cactcaatga tggagcatag gacaaggaat attcttcgag cagatatttg
     3601 ttgtgattcg tcgttaaggc ctggaacgga agattccata agaagtcatc cgggatgatg
     3661 cagagctttt tgatacccgc gagctcaccc tgagccggtt gaaccaacct ccgatagagt
     3721 tcacggccga agctttcaaa atcgggatgc ctgatcgcca gtctgctatt aagagtgttg
     3781 atttgcttgc gcagtgtttc tggtcttacc tcaatacgaa acgatttgag gagggtttct
     3841 tgtggtccgg cgcgcaacaa gaaaagatct ataccgtctt tgccgatcac gtattccagg
     3901 tacgcagtgt ctggctgaac tgtaaaccgc gcaagatcgc ttgcggtgat ttcggctgtt
     3961 cgccctaatc gcaccttcag atcgggatgt aatgcgtaca atgaatttcg gaaggtctga
     4021 tcttcacgga tagttcggtt cagttgctcc tggtcgcttg attgtgcttc gttgaagtcg
     4081 gaagcgtttc gtttacgtcg tgtgacttcg ctcctctcga cggttgaggg aaagggatcg
     4141 gacaccgaac gaaagacgtc aagcagaacg cgaccctttg ctcgttccgc aaagagcaac
     4201 gcctcggaga aattgttctg cttaagaagc agatcgatca gcatgcgata acttgaaact
     4261 ttatcctgaa agaagagctg acgctcgatt tcttcgccta caaccttgtg acgcatgagt
     4321 tcgacagtgt ctatcgcctg tttcaatgtc tcaatagcaa cctcagggcg actcatcgcc
     4381 gcgtatgttt ggccgattgc ggcgagtgca aggtaggtta gcttgggaga atgcattaaa
     4441 cgtgcgatct ctaatgctgc ctcagcatgg ccgagagatg aatcgaagtc acccatcgcg
     4501 aaccgaacct cggcaatacg ccaaagaagt tctgcttcgc gtatgcgttc ttttctctcc
     4561 aaagcgagta gcaagctgct attgaatatc tggagcgccg attgaaagtc gcgtttggcg
     4621 gcgaagacgg cgccgattcc ttcgccagct gcgatttgca cctcaaagtt atctgtcgct
     4681 tttgccagtt caaggctcgt ctgaaagagt gtgagcgcct cctcgaacct tttctggcgt
     4741 tgttgcacga ctcccaggtt gagcagcgat ctcgattctt ctctgcggtt cttgcccgca
     4801 cggtatcgtt gcagactgtc atgaaacttg gcccatgcct ggctgtaatc ttcctgctcg
     4861 acatataaat agccgatgtc attgaggagc gcggcttctt ggtttgattg gttaagagtt
     4921 gttgagagcg cgagggcccg gttgaggaaa gcgagccccc gcacatgatc gccagttgag
     4981 gtgtagaggc gaccaatcga tgcacagacg tctgccacgt aatagccgta ggtaggatgt
     5041 cttcgttcaa gctcatcaaa gagctctaag gagcgctgca agttgttaaa ggcgtcccaa
     5101 agattgcctt cgcgggttcc caattctccc agggtactga acgctccagc ggctccataa
     5161 gtatcgagtt cgttggtcgt tgagtcaggc gaacctaagg cccgccagag cttgatgcat
     5221 tcttcggaat agtttcgggc cgcttcgtag tcttgtgtga taagactgac ggttccgaga
     5281 tcactaaaga tgtagagaag atcatgaacg gagtccgcag ctgagaaagc cttcctgctg
     5341 ctcaaaaacg cttcttttgc cttatcgaac tgattgagtc cggaatagga acgcccaaga
     5401 ttgtggaaag tctttcctat cagattttga tcatgaaggc gcaatgccac ttcacacgct
     5461 aaacgatagg tttcgatccc ctgatctggc tgacggttgt gataggcgtg agcggcagca
     5521 tccatcaagc tttgccacag gttcggcgat ataaacggag agttgttttc aagcaagtag
     5581 ttgactcggt ccggattatt gccggcgtta attaaagcgg taacgagatc ctttgaggaa
     5641 tcgatccgct ctccgtctgt cgcactttga gaatgagt
//
LOCUS       DASSFP010000226         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_652214, whole genome shotgun sequence.
ACCESSION   DASSFP010000226 DASSFP010000000
VERSION     DASSFP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652214"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFH91_04905"
     CDS             complement(<1..359)
                     /locus_tag="VFH91_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862358.1"
                     /translation="MITAHHVSVQPRVVREADTLSAEGFEVRVIARQTDPELCRYDRE
                     LMESRSWLLETIELVRNGSSHQNWFKETLNSKLSKALFDVGICKGALAPRAYVRGMNR
                     LADLAKSKPADWFVAHT"
     gene            complement(403..1461)
                     /locus_tag="VFH91_04910"
     CDS             complement(403..1461)
                     /locus_tag="VFH91_04910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6862359.1"
                     /translation="MALDLSTGSVITSPRSTGNTVAVSILVSTFNGERFLKPALESLL
                     GQSFQDFELIVVDDGSTDRTQEILSAIDDHRLIVIRHPTNLGIAASQNDGLSSARGTY
                     IALQDHDDISDKDRLGKQFSFLEMNPGIGLVGSSGRVVDAQLRKLEEWAVPCSDIDLK
                     WDVLFKNPFLHTSLMVRRNEFKGVPYSRDAAFRFAEDFEWLSRFCERNTVANLPETLV
                     SWRRHTTQASSSIEVQKASAARIARRNIARLIGNPEVAAESWQALKKLLLWSPYEPVS
                     ISRHEVHKVSSLLVKLQKHFYRRHGFSAEEVSDHKSRTTKLIGRHFLALAYRRNGQRS
                     IGCRSALLSAGARFILST"
     gene            complement(1436..2197)
                     /locus_tag="VFH91_04915"
     CDS             complement(1436..2197)
                     /locus_tag="VFH91_04915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6862360.1"
                     /translation="MASLSSVVKQHPVGLRQNDLFRWAVSAGWRRVSDHRELNKRLRS
                     LKAACPSLEDVEGTFNTLRDYPEFMTVQKREEIVALLRLLSERRPSLMCEIGTSFGGT
                     LFQLARAADSAAKIISIDCGLSVVRSRVHARMAKKSQRIVCLRSDSTADDTIRRVKAI
                     LGDQKLDFLLIDGDHSYTGVVKDFANYSPLVASGGLIALHDILPDYQTKLGIDTENWT
                     GGVPQFWKELKHLYRTEEFLEDVNQDGYGIGLIHW"
     gene            complement(2201..3481)
                     /locus_tag="VFH91_04920"
     CDS             complement(2201..3481)
                     /locus_tag="VFH91_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862361.1"
                     /translation="MSDLAIRCEGLSKRYRLGQSERYKTLRDVISGAVTGPFKRRVNN
                     NGTRDSIWALEDVSFEVKRGEVVGVIGKNGAGKSTLLKILSRITKPTRGHANINGRVG
                     SLLEVGTGFHPELTGRENIFLNGAILGMRKSEIARKFDQIVEFAEVEKFVDTPVKRYS
                     SGMYVRLAFAVAAHMETEILLVDEVLAVGDAQFQKKCLGKIGNIASEGITVLFVSHSM
                     AAVQSLCSRGLLIDNGKLIVEGECRKVIEAYVSHSVQDCTASIELRTHSGRTKEAQPV
                     FSRVRIMNGNGEPTATLFAGEDLVLEFDLEPPHAISNPTLGIGVDNSLGFRIFSVITY
                     YSNFDWPKLEGPTTVRCTIPRINLLPGRYQLNISVGNYYNQLIDAIYNAASFAVEPSD
                     YFGTGRLPTQDLGVVLADSKWECLSSRRYQVVVS"
     gene            complement(3493..4398)
                     /locus_tag="VFH91_04925"
     CDS             complement(3493..4398)
                     /locus_tag="VFH91_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862362.1"
                     /translation="MIIFPTRVDVRPHSNSQISIHNYHLIAASPQSVRIQPATSWTTV
                     GLCELWQYRELLYFLTWRDIKVRYKQTALGAAWAVVQPLFMMLIFSLSFGKLAKVPSD
                     GFPYPIFTFCALLPWQLFAHALTESSNSLVGNQNLITKVYFPRLVVPISAVLGGLVDF
                     AIAFVILLAMMCYYGIFPGIQILALPAFILFAVLTALAVGLWLSALNVQYRDVRYTIA
                     FLIQFWLFATPVAYPSSLVPEKWRALYGLNPMAGVVEGFRWCLLGTASFPGHVVWASV
                     AVVVLLLVGGLYYFRRMEQEFADVI"
     gene            complement(4431..>6230)
                     /locus_tag="VFH91_04930"
     CDS             complement(4431..>6230)
                     /locus_tag="VFH91_04930"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET6862363.1"
                     /translation="GLKVEPVKENRGFYKETRLIDIAFQTTDPQVAQKVVNAIAETYV
                     FSNLEKNSEANSTTGDFLQKRIAELQQRIRSDEERLVNYAKNNQIISLNPNQNTVVER
                     LAGLNRQLLEAENDRKAAEAAYNAARLPGAANALADSEAKQANELDIKLAELRQKRAQ
                     LLVDATEEAPEVKEIDQQIAEVDRQVKDLRSRRSSTILTNLNTRYQETLQREQSLRKA
                     FDQQRAETLTQNEASINYRIIQQEIETNKTLLENLLQKSKENDVVLAGKANNISVVDY
                     ALVPDAPVGPNRARTVFVAFFLSIGLGVGVALFLEYLDDTVRSTEEVERMLHLPALAV
                     IPSVESTTRRRVLGASVALQKRSGNGAGNGSAELLMNVDGRSPLAEAYRHLRTSVLLS
                     TAGRSPKSLLVTSSLPGEGKTTTAVNTACSLAQTGVSVVIIDADMRRPRLQSIFGIRG
                     QLGLSSILSSDVTEPEILAMVMKDEVTGLSVLTSGPIPPNPAELLGSDQMRRLLGMLE
                     NHFTHVVVDSPPISSFTDGVLISTIVDGVLLVVHGGKSSRHIVRRSKQLLTDVGAKIF
                     GVVLNNINVQSPDYYYYHGYYGQKYYENEKVGA"
BASE COUNT         1553 a         1610 c         1530 g         1537 t
ORIGIN      
        1 tgagtatgtg cgacgaacca gtcggctggt ttgctcttgg caaggtcagc cagccggttc
       61 ataccacgca catacgcgcg tggggccaga gcgcccttac agattccaac atcaaaaagg
      121 gccttcgaaa gctttgaatt gagtgtttcc ttaaaccaat tctggtgaga cgagccgttt
      181 cgaaccagct ctatagtttc aagtagccag gatctcgatt ccatcagttc ccggtcgtac
      241 ctacaaagct cgggatcggt ttggcgcgcg atcacccgca cttcgaatcc ctccgcgctc
      301 agagtgtctg cctcacgaac aacgcgaggt tggacggaaa cgtgatgagc agtaatcaaa
      361 caaactcgca caagactaac tagtcgctac ttcgccgagc tgctaagtcg acaagataaa
      421 tcgtgcaccg gccgaaagca gagctgagcg gcaaccgata cttctttgtc cattcctacg
      481 ataagcgagt gctaaaaaat ggcgacctat gagtttagtt gtcctcgact tgtggtcgct
      541 aacttcttct gctgaaaaac cgtgacgacg atagaaatgc ttctgcagtt ttaccaggag
      601 tgaggaaacc ttgtgaactt cgtgccggct gatcgagact ggttcatagg gcgaccagag
      661 cagaagtttc ttcaacgctt gccatgactc agccgcaacc tcggggttgc caataagacg
      721 cgcgatattt cgccgagcga ttcgagcggc tgaagccttt tgaacctcaa tactcgaaga
      781 tgcttgagtg gtgtgtctcc gccatgacac aagagtttcc ggcaagttgg cgactgtgtt
      841 tctctcacaa aatcgggaca accattcgaa gtcttctgca aaacgaaacg cagcatcacg
      901 cgaataaggc acacctttga actcgtttcg acgcaccatc aacgaggtat gcagaaacgg
      961 gttcttgaat agcacgtccc acttcagatc gatgtccgag caaggaacgg cccactcctc
     1021 cagttttcgc agttgggcat caacaactcg accgcttgac ccgaccaagc cgattcccgg
     1081 attcatttcg agaaaagaga attgttttcc aaggcgatcc ttatccgaga tgtcatcgtg
     1141 atcctgtaaa gcgatatagg tacctcttgc cgaactgaga ccatcgttct gagatgcagc
     1201 aatcccaagg tttgtaggat gtcggatgac gatgagtcga tgatcgtcga tagcactcag
     1261 aatttcttgt gtacggtccg ttgagccatc atcgacaact atcagttcga agtcttggaa
     1321 cgactggccc aaaagactct cgagcgccgg cttcaggaat cgctcgccgt taaacgtgct
     1381 caccagaatt gagacggcta cagtgttgcc cgtggatcgt gggctggtta ttacactacc
     1441 agtggataag tccaatgcca tagccgtcct gatttacgtc ctccaaaaac tcctctgttc
     1501 gatacaagtg cttgagttcc ttccaaaact gcggaacacc tccggtccaa ttttccgtat
     1561 caatacctaa cttcgtctgg taatcgggaa gaatgtcgtg gagtgcgatt agaccgccac
     1621 tggcaacaag aggtgagtaa ttggcaaagt ctttgactac tccggtgtaa gaatgatctc
     1681 catcaatcag caaaaaatcg agtttttggt cgccgagaat cgccttcacc ctacgaatcg
     1741 tgtcgtcagc agttgagtca gatctaagac aaactatccg ctgcgacttc tttgccatcc
     1801 tcgcgtgtac tctcgagcga acgacggata gaccacagtc aatgctgatt atctttgcag
     1861 cgctgtcagc ggctcgggct aactgaaaga gtgttccgcc gaaggaagtg ccgatctcgc
     1921 acatgaggct tggtcttcgt tcggacagca gcctcaacaa agcaacaatc tcttcgcgct
     1981 tttgaacagt catgaactcc ggataatctc gaagagtgtt aaaggttcct tctacatctt
     2041 cgagagacgg gcacgcagct tttagcgaac gaagtctttt gttcagctca cgatgatcgc
     2101 tcaccctccg ccatccggct gaaacggccc accggaagag gtcattctgt cgcagaccga
     2161 cggggtgttg tttaactaca gaggagagac tagccacgga ttacgacacc accacttgat
     2221 accgtcttga agaaaggcat tcccacttgg agtcggctaa gactacgccg agatcttgag
     2281 tcggtagcct tccggtaccg aaataatcac ttggttcgac tgcgaatgat gcggcgttat
     2341 aaatcgcatc gatgagttgg ttgtaataat ttccgacact aatgttgagt tgataccgac
     2401 cgggcaaaag attgatgcgg ggaatggtac atcttacagt cgtaggtcct tcgagcttcg
     2461 gccagtcaaa gtttgagtaa taggtaatta cgctgaaaat ccgaaagccg agcgaattat
     2521 cgacaccgat tcccagtgtc ggattactga tcgcatgcgg aggctcgaga tcgaactcaa
     2581 gtaccagatc ctcgcccgca aacagggttg cggttggttc tccgttgccg ttcatgatac
     2641 gcactcgaga aaagaccggc tgtgcttctt tggtccttcc ggaatgtgtt cggagttcga
     2701 tgctagcggt gcagtcctgc actgagtgac tgacatacgc ttcaattact ttgcgacact
     2761 caccttcgac gattagtttt ccgttatcaa tcaaaagtcc tcttgaacac agcgattgga
     2821 ccgccgccat gctatgactc acgaatagaa ctgtaatacc ttcgctcgcg atgtttccga
     2881 tctttcctaa acacttcttt tgaaattgcg catcgccgac tgcgagaact tcatcgacga
     2941 gtaatatttc agtctccatg tgcgcggcga ccgcgaatgc gagccgcacg tacattccgc
     3001 tggagtatcg tttgaccggt gtgtcgacaa acttttcgac ttcggcaaat tcaacgatct
     3061 ggtcaaactt gcgcgcaatt tcggactttc gcattccgag tattgcacca ttcagaaaaa
     3121 tgttttctcg gccagtcagt tccggatgga acccggtgcc gacctcgagc agcgaaccga
     3181 cacgaccgtt gatgttcgca tggcctcgag ttggcttcgt gattcgcgac aggatcttga
     3241 gaagagtgct ctttcccgct ccgtttttgc cgatgactcc gactacttcg cctcgtttaa
     3301 cttcgaatga gacgtcttcg agggcccaga tcgagtcccg cgttccgtta ttgtttaccc
     3361 ggcgtttgaa cggaccagtc actgcgccgc tgatcacatc gcggagtgtc ttatatctct
     3421 cgctttggcc cagccggtaa cgcttgctta gaccttcaca acggattgcc agatctgaca
     3481 tttatctctc gatcaaatta cgtccgcaaa ttcctgttcc atgcgtctga agtaatacag
     3541 accgcctacc aacaggagta cgacgaccgc gactgaggcc cagactacgt gtccgggaaa
     3601 tgacgctgta ccgagtaaac accaacggaa accttccaca acgccggcca tcgggttaag
     3661 gccataaagc gcacgccatt tttcaggtac aagagaactc ggataagcaa cgggagtggc
     3721 gaacaaccag aactgaatca gaaaagcaat ggtgtagcgt acatcacgat attgaacatt
     3781 gagagcggac aaccatagac caaccgcgag cgcggtcaag actgcaaata agatgaaagc
     3841 cggaagcgca aggatctgaa ttcccgggaa gatcccgtaa tagcacatca tcgcgaggag
     3901 gatgacgaac gcaatcgcga aatcaaccaa accaccgagc accgccgaga tgggtaccac
     3961 caaccgcggg aaatatacct tcgtgatcag attctgattg ccaacgagac tgttactcga
     4021 ttcagtgaga gcgtgtgcaa atagttgcca tggcaacaat gcacagaaag taaagatcgg
     4081 ataaggaaat ccatccgacg gcacttttgc cagtttgccg aacgagagac taaagatcag
     4141 catcatgaaa agaggttgga ctacggccca ggctgcaccg agcgccgtct gtttgtagcg
     4201 tactttgata tcgcgccaag tgaggaaata gagaagttct cgatactgcc agagttcgca
     4261 aagaccgact gtggtccaac tggtagctgg ttgaatacga actgattgag ggctggcggc
     4321 gatcaagtga taattatgaa ttgatatttg agaattacta tgtggtctaa catcaactct
     4381 cgtcggaaaa attatcaatt atcaactgtc aaatgataat tgatacttat tcaggctccg
     4441 accttttcat tctcgtagta cttctggcca tagtagccgt gatagtagta gtagtctggc
     4501 gattgcacgt tgatattatt cagtaccact ccgaaaatct tagctccgac atctgtcagt
     4561 agttgtttcg accgtcgaac aatatggcgt gaactcttgc ctccatgcac caccagcaag
     4621 actccatcga ctatcgtaga aatcaacacg ccatctgtga acgaactaat tggcggggaa
     4681 tcgaccacga catgcgtgaa gtgattttcg agcattccaa gcaaccttcg catctggtcg
     4741 gaaccaagca attcggccgg gtttggagga attggaccag acgtgagcac actcaagccc
     4801 gtcacctcat ccttcatcac catagccagt atttccggct cagttacatc actcgacaga
     4861 attgagctca agccgagttg cccgcgaatt ccgaagattg attgcaaacg aggcctgcgc
     4921 atgtcggcgt caatgatgac gacactcact ccagtctgcg ccaaactaca cgctgtgttt
     4981 actgcagttg ttgtcttccc ttctccaggt aaacttgagg ttaccaggag agattttggt
     5041 gaacgaccgg cagtggaaag aagaacagaa gtacgtaagt gacgataggc ttcggcgagc
     5101 ggtgaacgtc catccacatt cattaacagc tcagcactac cattgccggc gccgttcccg
     5161 ctgcgttttt ggagagcaac tgaggctccg agtacacgac gtcgagttgt gctctcaacc
     5221 gacggaataa ctgccagcgc tggcaggtgc agcattcgct caacctcttc agtagagcgc
     5281 acggtatcgt caagatattc aagaaacaga gcaacgccaa cgcccaggcc aatagaaagg
     5341 aagaatgcga caaataccgt tcgtgctcga tttggtccaa ccggagcatc gggcacaagc
     5401 gcgtaatcga cgactgagat gttgttcgcc ttgccagcga gcactacgtc gttctctttt
     5461 gatttttgta agaggttctc gagcagcgtc ttgttggtct caatctcctg ttgaattatg
     5521 cggtaattga ttgacgcttc gttttgcgtc agcgtttcag ctcgttgctg gtcgaaggct
     5581 ttgcgcaagc tctgctcacg ttgcagtgtc tcctgatacc gcgtgttgag gtttgttagg
     5641 attgtagagc tgcgacgtga acgtaagtcc ttcacctgcc ggtccacttc cgcgatttgc
     5701 tgatctattt ctttgacttc gggggcctcc tcggttgcgt cgaccaggag ttgcgcgcgc
     5761 ttttgtcgca attccgccaa cttaatatcc agttcatttg cctgtttagc ctcactgtcg
     5821 gccaaagcgt tggcggcacc cggaaggcgc gcggcgttgt atgcggcctc ggcagccttg
     5881 cgatcgtttt ctgcttcgag tagctgtcgg tttaatcctg ccagtctctc aacgaccgta
     5941 ttttgattgg gattaagtga gattatctgg ttgttcttcg cataattgac cagacgttct
     6001 tcgtcactgc gaatgcgctg ttgcagttcg gcaattcgct tctgcaggaa atcgccggtg
     6061 gtactgttag cttcggaatt cttctccaaa ttcgagaaaa cgtaagtctc cgcaattgca
     6121 ttaacgacct tctgggcaac ctgaggatca gtagtctgga acgcgatatc gatcaaacgc
     6181 gtctctttat agaacccgcg attctctttc acaggttcaa ctttgagacc
//
LOCUS       DASSFP010000227         4162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_954429, whole genome shotgun sequence.
ACCESSION   DASSFP010000227 DASSFP010000000
VERSION     DASSFP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954429"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFH91_04935"
     CDS             complement(<1..554)
                     /locus_tag="VFH91_04935"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6862364.1"
                     /translation="MKICRIKHSSFPEPRFGVIEGDDVLVLPSDFTFLNSQNRKFESK
                     LKLDDVSLVAPVTPSKIVCVGRNYREHAAELGNKMPDEPLLFLKPPSSLIASRDRIEL
                     PAASQQVEHEGELGVVIGKQARKLSDQDNPLDYIFGYTCVNDVTARDLQRKDVQFTRG
                     KGFDTFCPDGPWVGTDLDAQATQV"
     gene            complement(559..1710)
                     /locus_tag="VFH91_04940"
     CDS             complement(559..1710)
                     /locus_tag="VFH91_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862365.1"
                     /translation="MNHRYAVHITPSRSYLTHDSRKSFLPFLAGICLLAICVAGCGKR
                     RPPLQQVERVQQRTELLSGAQQGNEVILSWPAPQRNAPDDSVQSIRRIDVYRLAEKPG
                     SPLALTEEEFAARSTLIGSVTYDQIKTAGDKITYTDILELAGQPTRLRYAVRYVNASG
                     QRAAFSNFLGIEPAAKIARPPTLRKTDKELTEDAITITWTAPDANIDGSIPVNLLGYN
                     VYRMDDAQTQPNQEPINATLVSGTQYADKSFKFGQTYKYVVRSVSLGTGGANVESLNS
                     NSISITARDTFAPSAPQGVDVAGSPGRLSIFFAANPEPDIAGYNIYRSTDPNLPKSSW
                     TRLSQSLLTRTTYLDERVETGKRYYYYVTAVDTAGNESAPSETDSEVVP"
     gene            complement(1717..3144)
                     /gene="hslU"
                     /locus_tag="VFH91_04945"
     CDS             complement(1717..3144)
                     /gene="hslU"
                     /locus_tag="VFH91_04945"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET6862366.1"
                     /translation="MVIYLAGEVDEEMRLDEMSPRQIVTELDKHVVGQRNAKRAVAVA
                     LRNRVRRQKLAPDLAVDVMPKNIIMIGSTGVGKTEIARRLARMSNSPFLKVEASKFTE
                     VGYVGRDVESMIRDLTEISVEMTRQEKSEEVAEKAELNVEERILDLLLPPPRSISGFY
                     DFDAQDGEEESTEVDSTARDEQFQRTRDKLRTQLRSGKLDNRLVELEVREKNFPVIEL
                     AGPQGVEEMGINMKDMLGNLFQGRTKRRKMRVDEAMEYLMQEEEERLIDMDQVARAAI
                     ERVESAGIIFLDEIDKIAGRESGHGPDVSREGVQRDILPIIEGTTVNTRYGMVRTDHI
                     LFIAAGAFHVSKPSDLIPELQGRFPIRVELESLTIDDFKRILTEPRNALIKQYQAMME
                     TEGVSIEFTLDAIDSIARFAAQVNQQTENIGARRLQTIMEKLLEEISFEGPDLEPKQQ
                     RIDAAYVEGMLSETVKDQDLSRYIL"
     gene            complement(3175..3717)
                     /gene="hslV"
                     /locus_tag="VFH91_04950"
     CDS             complement(3175..3717)
                     /gene="hslV"
                     /locus_tag="VFH91_04950"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET6862367.1"
                     /translation="MPKENKVRSTTVLLVRKDSQIALAGDGQVTMGETVMKASAKKVR
                     RLYNGQILAGFAGATGDAFSLLTRFESKLEQYHGNLERAAIELSKEWRTDKMLRHLEA
                     LLVVADTKSSFLLSGNGDVIAPDEGILAIGSGGSYALAAARALVKHTELSARDIAVEA
                     LRIAGEICIYSNQNIVVEEL"
     gene            3851..>4162
                     /locus_tag="VFH91_04955"
     CDS             3851..>4162
                     /locus_tag="VFH91_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET6862368.1"
                     /translation="MQRRPRIGITMRIEQTTDRFYLGRHYSEAVAAAGGVPLHISLIP
                     DVRYIDAVMEGLDGILLPGSDSDVDPLRYGQDPHPNLGTVQPLKDETDLLVITAVERL
                     NL"
BASE COUNT          966 a         1065 c         1036 g         1095 t
ORIGIN      
        1 attacctgcg tagcttgtgc gtccaaatcg gtacctaccc atggtccatc ggggcaaaaa
       61 gtgtcgaacc ctttgccacg cgtaaactgg acgtccttac gctgcaggtc acgcgcggtc
      121 acatcgttaa cgcaagtgta cccaaagatg taatctaatg gattgtcctg atctgataat
      181 tttctagctt gctttcctat gacaactcca agctctcctt catgttccac ttgttgtgaa
      241 gctgcgggta attcaatgcg atctcgggac gcaattaaag acgacggagg ttttaaaaag
      301 agaagtggct catcaggcat tttattgcct aactcagctg cgtgctctcg atagtttcta
      361 ccgacacaaa caatcttgga tggagtcacg ggtgcaacca gacttacatc gtccagttta
      421 agcttagact caaacttgcg gttttgagaa tttagaaacg tgaaatcaga gggcagcaca
      481 agtacatcat caccctcgat cactccaaat cgaggctccg gaaaactcga atgttttatg
      541 cgacaaatct tcatgtgatt acggaacaac ttcagagtct gtctccgatg gggcactttc
      601 gttaccggcg gtatccaccg ctgtcacgta gtaatagtat ctcttaccgg tctcaacgcg
      661 ttcatccagg tacgttgtac gagtcagcag agactggctg agtctcgtcc aggatgactt
      721 cggcaagttt ggatcagtag atctataaat attgtatccg gcgatgtcag gttccgggtt
      781 cgctgcaaag aaaatagaaa gcctgcctgg ggagcctgct acgtcaactc cctgtggtgc
      841 tgaaggcgca aacgtgtcgc gcgccgtaat cgaaatcgaa ttggagttca aactctcgac
      901 gttagctccc ccggtaccca gcgatactga acggaccaca tacttataag tttgtccaaa
      961 tttaaaggac ttgtctgcgt actgagttcc agaaaccaat gtagcgttaa tcggctcctg
     1021 gttcggctgc gtttgtgcgt cgtccattcg gtaaacgtta tagccgagaa ggtttacggg
     1081 aattgagcca tcgatgtttg catccggtgc agtccacgta attgtgatcg catcttcggt
     1141 cagttcttta tcggtcttac gcaaagttgg tggtcgagca attttcgcgg cgggctcgat
     1201 gccgaggaaa tttgaaaatg ctgcacgttg tccggatgca tttacatagc gaacagcgta
     1261 acgcaagcgt gttggttgtc cggccagctc aagaatgtcg gtataagtaa tcttgtcgcc
     1321 ggcagtcttg atctgatcgt aggttacgga tccaattagt gtggaccgtg cggcaaactc
     1381 ttcctcagtc agggccagag gtgaacccgg tttctcggca aggcggtaaa catcaatgcg
     1441 tctaatactc tgtacgctgt cgtcaggagc atttcgctgg ggagccggcc agctgaggat
     1501 tacctcattt ccttgttgcg cacccgacag caactctgtt cgttgttgga ctctttcgac
     1561 ttgctgtaac ggaggacgtc gcttgccgca acctgcaaca caaatggcca gcaggcatat
     1621 cccggcgaga aaaggtagaa aagactttcg agaatcgtgc gtcaaataag accgtgacgg
     1681 cgtgatgtgt acggcgtagc ggtgattcaa agttttttac aggatataac gactgaggtc
     1741 ctgatcctta acagtttctg agagcatgcc ttcgacataa gcagcatcaa ttctttgctg
     1801 cttgggctct agatctggcc cttcaaaact gatctcctcc aggagcttct ccatgatcgt
     1861 ctgcagccgt ctggcgccga tgttttcagt ttgctgattt acctgggcgg caaatcgggc
     1921 aatcgagtcg atagcgtcaa gtgtaaactc aatcgaaaca ccttcagttt ccatcatggc
     1981 ctggtactgc ttgatcagtg catttcgtgg ttcggtgaga attcgtttga agtcatcaat
     2041 ggtcaacgat tcgagttcaa ctctaatcgg gaatcgaccc tggagttcag gaataaggtc
     2101 ggatggcttg gagacatgaa atgcgccggc ggctatgaaa agaatgtgat cagttcgaac
     2161 cattccgtag cgggtgttaa cagttgttcc ttcgatgatc ggaagaatat cgcgctgcac
     2221 gccttcacgg gagacatctg gtccatgtcc ggattcacgg ccggcaatct tgtcgatctc
     2281 atctaaaaag atgatgccgg cactttcgac gcgttcgatt gctgcacgcg ccacctggtc
     2341 catatcgatg aggcgttctt cttcttcctg catcaaatac tccatcgcct cgtcaacccg
     2401 cattttgcgc cgcttggttc gtccttgaaa cagattgccc aacatgtctt tcatgttgat
     2461 tcccatctct tcgacgcctt gcgggcctgc cagctcgatc acaggaaaat tcttttcacg
     2521 tacctcgagt tctaccaacc ggttatccag ttttccgctg cgtaactggg tgcgaagttt
     2581 atcgcgggtg cgctggaact gctcatcacg tgcagtagaa tcgacttcgg ttgactcttc
     2641 ctctccatcc tgggcgtcaa aatcatagaa gcctgagata gagcggggag gtggtagcag
     2701 caagtcgagt attcgttcct cgacgttcag ttctgctttt tctgccacct cttcactttt
     2761 ttcctggcgt gtcatctcta cagagatttc agtgaggtcc cgaatcatgc tttcgacgtc
     2821 gcggccaaca tacccaacct cagtaaactt tgaagcttcc actttcagaa acggtgagtt
     2881 cgacatccgc gccaatcgac gagcaatttc agtttttccc acaccggttg agccgatcat
     2941 aatgatgttc ttcggcatca cgtcgaccgc cagatcaggc gccagtttct ggcggcgtac
     3001 tcggttgcgt agcgcaaccg cgacagctcg cttggcgttg cgctgcccga cgacatgttt
     3061 gtctagttcg gttacgatct ggcgaggcga catctcgtcg agtcgcattt cttcatcaac
     3121 ttctccagcc aagtaaataa ccattgctat tgccttaaag gaacctctat ttcgttacag
     3181 ttcttcgact acgatgtttt gattggagta aatacaaatt tcaccagcaa tgcggagagc
     3241 ttccactgca atgtcgcggg ccgagagctc agtgtgtttt accagtgccc gggcggcagc
     3301 aagcgcatag gatccgcccg atccgattgc cagaattccc tcgtcagggg cgatgacgtc
     3361 gccattaccc gacaaaagaa atgagctctt agtgtcggcc accacgagca gggcttccag
     3421 atgccggagc atcttgtcgg tacgccattc tttgctgagc tcaatagcgg cccgctccag
     3481 attgccatga tactgctcga gcttactttc aaaacgagtc aataacgaaa aagcgtcgcc
     3541 cgtcgctccg gcgaaaccag cgagtatctg tccgttataa aggcggcgca cctttttagc
     3601 gctggccttc atgacagttt cacccattgt tacctgtcca tcaccggcga gcgcgatttg
     3661 cgagtcttta cgcacaagca ggacagtggt actgcgcact ttgttttctt tcggcatggc
     3721 tatttgccta gcactatagg aactcgtttc aagctaagtc aaggagcctt acgtaagtgg
     3781 caatcacgca acggactgaa agttgggtta tcatacacag tcgaaatttt gataggttaa
     3841 gtggagcaat atgcaaagac gaccacgcat cggtattacg atgcgaatag agcaaacaac
     3901 agatcgcttt tatctgggcc ggcattacag cgaggccgtt gcagccgcag gcggtgtgcc
     3961 gcttcacatc agtctcatcc ctgacgttcg ctacatcgat gcagtgatgg aaggcttaga
     4021 tggcatcttg ttgcccggca gcgattctga tgttgatccg ttgcgctatg ggcaggatcc
     4081 tcatccgaac ttaggtacgg tgcagcccct caaagacgaa acagacctac tggttattac
     4141 tgcggttgaa cgtctgaacc tg
//
LOCUS       DASSFP010000228         8611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1099657, whole genome shotgun sequence.
ACCESSION   DASSFP010000228 DASSFP010000000
VERSION     DASSFP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099657"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="VFH91_04960"
     CDS             <1..1675
                     /locus_tag="VFH91_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET6862369.1"
                     /translation="EVAECYSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHAT
                     CATYAQNMAFNVRPPAMAEWIVNLGEAAEYMFDHNIFQDNLVGVDFCEQMVRETNPSV
                     WEKAKTKQAPHAADHGYRTIADIMTALNPFSGEFYRETLQVSRYTREMFCLMEGRHVH
                     PSTLYPGGVGTVPTVQLFTDYLVRLMRYCEFMKKVVPLHDDLFDFIYEAMPGYEKVGQ
                     RRILLGCWGSFQDPSVCDYTYKNMNEWGNAMYVTPGVIVDGKLVTTDLVDINLGIRIL
                     LGSSYYDDWENSETFVKADPLGNPIDRRHPWNQTTIPRPQKRDFDNNYTWVMSPRWYH
                     EGEPGDKKYLALDTGGGPIARFWATALAGKVDIGYVKATGHSVKIYLPKTAMKPEVEF
                     EWKIPRWSNAIERDRARTYFQAYAAAAALYFTEQAMAELHAGRTKTWSDFKVPDEAIG
                     CGFHEAVRGVLSHHVVIRDGKIANYHPYPPTPWNANPRDIYGTPGPYEDAVQNTPIFE
                     ENGPANFKGIDIMRAVRSFDPCLPCGVHMYLGNGKVLETHHSPMFGMVK"
     gene            1700..2578
                     /locus_tag="VFH91_04965"
     CDS             1700..2578
                     /locus_tag="VFH91_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET6862370.1"
                     /translation="MTSGPSKIEELIHNIEHLRDPEARENAIALVQALMEFHSSALER
                     LMDLIAEKGEPGFSIFDSFATDETVGSLLLLYGLHPLSLETRVTNALDKVRPYLSSHG
                     GSVELVEIVEDNVWVKLEGSCKTCPSSAMTLKLAIEDAIYAAAPDVVAIHAEGVTAAP
                     AETGFVQIGKPNGRAKVKESWRDVSSLVGLSDNSLEFADVDGHSVLFCRLGDALYAYG
                     NNCPGCGNELRDALLTSTTLMCNSCRQRFDVIRAGRGLDQPKLHLSPLPLLLENGRAR
                     IALPPLSMSQVPLTPG"
     gene            2698..3381
                     /locus_tag="VFH91_04970"
     CDS             2698..3381
                     /locus_tag="VFH91_04970"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HET6862371.1"
                     /translation="MSEIQVPNDEVSIDVQQPASGNASDAFNLLRRFVRRRATAERCD
                     MCSAELPVEHSHLIEPPTRQIVCACQACSILFSGSSDQRYRRIPRRIKFLQDFSLTEA
                     QWDSLMIPIGMAFFFRSSLDQRVIALYPSPAGATESLLDLQAWNEIVTGNQTLSEMEL
                     DTEALLVNRVNGANDYYLVPIDECFKLVGLIRTKWRGLSGGQDVWNAIEKFFNELKGR
                     SNLVGKTDA"
     gene            3374..4054
                     /locus_tag="VFH91_04975"
     CDS             3374..4054
                     /locus_tag="VFH91_04975"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HET6862372.1"
                     /translation="MPDLDFKIESAEAVTFAAAPLLAFQLSITNANPGELIQSIMLRC
                     QVQIETTRRKYDPPEQEQLSDLFGEPDRWAQTLRPMLWTHTNTVVPPFHDQSTVALPV
                     PCTFDFNVAATKYFAALNEGEVPLNLMFSGTVFYEAADNSLQAEQIPWDKEARFRLPV
                     SVWKEMINLYYPNSAWLCVRRDVFDRLSRFKTQQGIPTWEQTLDLLLPPGTESKDDII
                     VVEESLPS"
     gene            4051..5142
                     /locus_tag="VFH91_04980"
     CDS             4051..5142
                     /locus_tag="VFH91_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862373.1"
                     /translation="MSATAESKRERSLVERVAAAVLYEGYVLYPYRPSAVKNQQRWNF
                     GVVCPESYSVAQRGTEASLMQTECLLVADSSTRLTVSVKFLQLISREVYASVGPSEGS
                     ESKIDRTNFQPVSSLEHDGHLYNTWQEAVEREVGPISIDTGNFAKATESRAFEFEANE
                     QVEELSTSEDGPVVGLVTRKQSSLSGLVSLVVEPCANGGINGLHKVRVQITNTTTFEN
                     AEQKSRDEALLSSFVSTHTVLTTKGGEFISLLDPPAAVNDMAAECKNVGTYPVMVGAD
                     GEKDCMLSSPIILYDYPQIAAESPGDLFDGTEIDEILTLRILTLTDEEKREIRASDDR
                     ARRILERTEMLPLEQFMKLHGAMKGVNRK"
     gene            5144..5467
                     /locus_tag="VFH91_04985"
     CDS             5144..5467
                     /locus_tag="VFH91_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862374.1"
                     /translation="MNEWEWQLLEEKQPLDQILVNGVSVKCGDRVILRPKGRSDILDL
                     VLVGKIAVIEAIEQDYEGQAHVAVVIEDDPGRDFGVMRQPGHRFFFSTAEVEPLVESR
                     STENL"
     gene            5464..6012
                     /locus_tag="VFH91_04990"
     CDS             5464..6012
                     /locus_tag="VFH91_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HET6862375.1"
                     /translation="MTSRVLVAGIGNIFMGDDAFGCEVIRRLAGFQFPENVRIFDFGI
                     RGFDLAYALMEGYDLTILVDAVPRGEPAGTVYTIEPDLRQLEQPDQANLVVETHGMNP
                     MKVLQLVNSMGGTFKKILLVGCEPESFGPEEGQMGLSPSVAASVDRAVQVINDLISEF
                     LGNPALWEANKNGQEQWVSERV"
     gene            6213..6434
                     /locus_tag="VFH91_04995"
     CDS             6213..6434
                     /locus_tag="VFH91_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862376.1"
                     /translation="MTNNGSESKDILYLVGGFALLVAGAGLIMAHPEIRKQVRAGLDR
                     VIPGLPGNLGSSLSTVVPDVQRYLKIRSM"
     gene            6439..7002
                     /locus_tag="VFH91_05000"
     CDS             6439..7002
                     /locus_tag="VFH91_05000"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HET6862377.1"
                     /translation="MKNEGSLVKGVAAGMAGGLVASAVMNQFQSALSKLMSGDERSHG
                     AQSQQPGSPEHGIGKALQERGSDDPDDNAAVRAANAVEEFVFNERLTKTEKETAGTVA
                     HYAMGAASGAIYGACAELAPGVSIGEGVPFGAAVWLIADEAIVPAIGLSKSAKNYPLS
                     THAYALASHLVYGLTTELVRKAVRKAL"
     gene            7009..7584
                     /locus_tag="VFH91_05005"
     CDS             7009..7584
                     /locus_tag="VFH91_05005"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6862378.1"
                     /translation="MATELVSALAQYRILPEESTFTVQAFAEGLFSAFGHDPVIAVRS
                     FNGNAEFMPETFEKAALHLSVDASSLVVANEVKEKDRLEIENTMRREVLEVQTYPEIK
                     FDSNNVALNRLGPGRYRARLIGDLSLHGVTQKNVWITAEVTLSAEMLRAKGEYQLRQT
                     DFKVKPVSVAGGTLKIKNELKCAFDICAKPA"
     gene            7587..7964
                     /gene="hypA"
                     /locus_tag="VFH91_05010"
     CDS             7587..7964
                     /gene="hypA"
                     /locus_tag="VFH91_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET6862379.1"
                     /translation="MHELSIAMSMVEMATEECQRLGAERVTALHLKVGLLSGVVKEAL
                     IFSYEVVCAGTPLQGSKLVIEDVPVKVYCPRCNSKKDLDSIQLFACPACGELTPEVLE
                     GRELQVVAMEIEERQEVAEAVMA"
     gene            7961..>8611
                     /gene="hypB"
                     /locus_tag="VFH91_05015"
     CDS             7961..>8611
                     /gene="hypB"
                     /locus_tag="VFH91_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET6862380.1"
                     /translation="MSTPRLVEVRQNVLKQNDLLARSLRKRFADAGVSVLSLVSTPGA
                     GKTALLEHTLSSLRETGLQVAALVGDLATDNDAERLARSQVPVRQITTGTVCHLDASM
                     VEKALGDWDVEKLDILFIENVGNMVCPSSYDLGENLRIVLLSTTEGEDKPLKYPTIFN
                     TSDIAVITKMDLASVVGFDLEAARTNIQRVRPGMKTMEVSVKTGEGIIEFLDLIVSH"
BASE COUNT         2135 a         2001 c         2397 g         2078 t
ORIGIN      
        1 tgaggtggca gagtgctaca gcacttcttc aatctttcgc ggctacagca ttttcatgaa
       61 aggaaaggat ccgcgcgacg ctcacttcat tacaagtcgc atctgcggga tctgtggaga
      121 caatcacgcc acttgtgcca cctacgctca aaacatggcc ttcaatgttc gtccgccggc
      181 gatggcggag tggattgtga acctcggtga agccgccgaa tacatgtttg atcacaacat
      241 cttccaggac aatctggttg gtgtcgactt ctgcgagcag atggttcgcg agaccaaccc
      301 gagtgtctgg gagaaggcca aaaccaagca agcgccccat gccgccgacc atggttatcg
      361 aaccattgcc gacatcatga ctgcgcttaa ccctttcagt ggggagtttt atcgcgagac
      421 tttgcaggtg agtcgctaca cgcgtgagat gttttgtttg atggagggtc gacacgttca
      481 tccatccaca ctgtatccgg gtggtgtggg aacggtacct acggtccagc tctttacaga
      541 ttatctcgtg cgcttgatgc gttactgcga attcatgaag aaggtagttc ctttgcacga
      601 cgatctgttc gattttattt atgaggccat gcccggttac gaaaaggtgg gccaacgtcg
      661 catccttctc gggtgttggg ggtcgttcca ggatccatcg gtctgtgact acacctataa
      721 gaatatgaac gaatggggta atgcgatgta cgtgaccccg ggcgtcattg tcgatggaaa
      781 actggtgacg actgatcttg ttgatataaa cctaggcata cgaattctcc tgggaagttc
      841 ttattacgac gattgggaaa actcggaaac gtttgtcaaa gccgatccac ttggtaatcc
      901 gatcgatagg agacatccct ggaaccaaac aacaataccg cggccgcaaa aacgagactt
      961 cgataacaac tacacctggg ttatgtcgcc tcgttggtat cacgaaggtg agccgggcga
     1021 taagaagtac ctggcgcttg atacaggtgg cggtccaatc gcgcgtttct gggctacggc
     1081 tctcgccgga aaagttgata ttggttatgt caaagctacc ggtcatagcg tgaagattta
     1141 tcttccaaag actgcgatga agcctgaggt ggagtttgag tggaaaatac ccaggtggag
     1201 taatgccatc gagcgcgatc gggcccggac ctattttcag gcttacgccg ccgccgccgc
     1261 actttatttc acggagcagg caatggcgga attacacgct ggtcgcacca aaacatggtc
     1321 agacttcaag gtgcctgatg aagcgattgg ttgcggtttc catgaggccg ttcgtggagt
     1381 gttgtcacac cacgtagtga ttagagatgg caagatcgcg aactatcacc cttatccgcc
     1441 aacaccgtgg aacgctaacc cgcgtgacat ctatggaact cccggtccat acgaggatgc
     1501 ggtgcaaaac actccaatct tcgaagagaa tggaccagcg aatttcaagg gcatagacat
     1561 catgcgggca gttcgcagtt tcgatccctg tttgccctgc ggtgtccata tgtatttggg
     1621 taatggaaag gttttagaga ctcatcattc gccgatgttt gggatggtga agtgatagaa
     1681 gctcttaagg aggtcaagtt tgacgtcagg cccctcaaag attgaagaac tgattcacaa
     1741 cattgagcat ttaagagatc ccgaggctcg cgaaaacgct attgctttgg tccaggcgtt
     1801 aatggaattc catagctcgg cgttggaacg tctcatggac ttgatcgccg aaaagggtga
     1861 acccgggttt tcaatttttg actcttttgc gacagacgaa actgtcggaa gcttgctctt
     1921 gctgtatggc ctgcatccgc tttcattgga gacccgagtt acgaacgctc tagacaaagt
     1981 gcgtccctat cttagttcgc acggtggaag tgtcgagttg gtcgaaatcg tggaagacaa
     2041 cgtctgggtc aaactcgagg gaagctgtaa gacttgtccg tcctccgcga tgactctaaa
     2101 gcttgcgatc gaagacgcta tttatgctgc cgctcctgat gtcgtcgcga tccacgccga
     2161 aggcgttacg gccgcacctg cagaaactgg ctttgtccaa attggaaaac ctaatggaag
     2221 agcaaaagtg aaggagtcct ggcgcgacgt gagcagtctg gtcgggctca gcgacaactc
     2281 gctcgagttt gcggatgtag acggacattc cgtgttgttc tgccgtctgg gagacgctct
     2341 ttacgcatac ggaaataact gcccgggctg tggtaatgaa ttgcgagatg ccttactgac
     2401 gtcgaccact ctgatgtgca acagttgtcg gcaacggttc gacgtgattc gtgccggccg
     2461 tggcctggac cagccgaaac ttcatctcag ccctttgcct ctattacttg agaacggacg
     2521 tgcgcggata gctttgcccc cgttgtcaat gagtcaggtg cctcttacac ctggttaact
     2581 gccagaggtg agcgcacatg gaggacataa aaaacacgat gccggcacat gcgcctaaga
     2641 attagtaggc tgttgccaac gattttcatc gagaacggag tagatcgaac atacaacatg
     2701 tcagaaattc aggtcccgaa tgatgaagtc tcgatcgatg tgcagcagcc ggcctccgga
     2761 aacgcgtccg acgccttcaa cctactacgg cgatttgttc gacggcgggc aaccgcggaa
     2821 cgatgcgaca tgtgcagcgc tgaattgccg gtcgagcact cacatttgat cgaaccgcct
     2881 acaagacaaa tcgtttgtgc ctgtcaggca tgttctatcc tttttagcgg aagctcggac
     2941 cagcgttaca gacgcattcc tcgacgcatc aagttccttc aggacttttc tttaacagag
     3001 gcgcagtggg atagtctgat gattcccatt ggaatggcgt tcttttttcg tagcagcctg
     3061 gaccaaagag tgattgctct gtatccgagc ccggccggcg ccacggaatc gcttcttgat
     3121 ctacaggcct ggaatgagat cgtaactggt aaccaaactc ttagcgagat ggaactggat
     3181 actgaagcat tactcgtcaa ccgggtgaat ggcgccaacg attattacct cgttcccatt
     3241 gatgagtgct tcaagttggt tggtttgata cggacgaagt ggcgaggttt gtccgggggg
     3301 caagacgtct ggaatgcaat tgaaaagttt tttaatgagc tcaaaggaag gtcgaacctg
     3361 gtagggaaaa ccgatgcctg atcttgactt taagattgaa tctgctgaag cggtaacttt
     3421 tgctgccgct ccattactag cttttcaact gtcaattact aacgcgaacc ccggcgaact
     3481 gattcagtcg atcatgttgc gctgccaggt ccagattgaa actacgcggc gaaagtatga
     3541 tccaccggaa caggaacaac tttctgattt gttcggcgag ccggatcgat gggcgcaaac
     3601 tcttcggcct atgctttgga cccacacgaa cacggtagtg cccccatttc acgatcagag
     3661 tacggtcgca ttacctgtgc cgtgtacatt cgatttcaat gtagccgcta ccaaatattt
     3721 cgcagcttta aatgaaggag aggtgcctct gaatcttatg ttcagtggca cggtgttcta
     3781 cgaggcggct gacaactcac tgcaagctga acagattccc tgggacaaag aagctcgctt
     3841 tcgcctgccg gtttcggtgt ggaaagaaat gatcaatctc tattacccga actcagcctg
     3901 gctatgtgtg cggagagatg tcttcgatcg gctttcgcgc ttcaagacgc aacaaggcat
     3961 tccgacctgg gaacaaacgc ttgacttgct tctgccgccc gggaccgaaa gtaaagacga
     4021 tattattgtc gtcgaggaaa gcttaccttc gtgagtgcaa ctgctgaatc aaagagagag
     4081 agatctctgg tagagcgtgt cgctgccgcg gtgttgtacg agggctatgt actctacccg
     4141 tatcgtcctt ctgcggttaa aaaccaacaa cgttggaact ttggcgtggt gtgccccgaa
     4201 tcctacagcg tcgcgcagag aggtaccgaa gcgtctctaa tgcaaacgga atgtctgctg
     4261 gtagcggatt cttctactcg tctaaccgtg agcgtcaaat ttcttcaact gatttcgcga
     4321 gaagtttacg cctccgttgg tccatcagag ggctccgaga gcaaaattga ccgtacaaac
     4381 ttccaaccgg tcagctctct tgagcacgat ggtcatcttt acaatacatg gcaggaagct
     4441 gttgagcgtg aggttggtcc aatctcgatc gatactggca actttgcaaa agcaaccgag
     4501 tcgcgcgcct ttgaatttga agccaacgag caagtagaag agctaagcac ctctgaagac
     4561 ggaccagtcg ttggtctagt gacgcgcaag caaagctcct tgtccggatt ggtaagtctg
     4621 gtggttgagc cctgtgcgaa tggcgggatc aacggccttc ataaggttag agtccaaatc
     4681 actaatacaa caacctttga gaatgcggaa caaaagagcc gtgacgaagc tctgctgagc
     4741 tctttcgtct cgacgcatac cgttcttact acaaagggtg gggaatttat atctctgttg
     4801 gatccgcctg cagcagtgaa tgacatggca gcggaatgca aaaacgtcgg cacctatccg
     4861 gtcatggttg gagcagacgg agaaaaggat tgcatgctct cttcgccgat catcctttac
     4921 gactatccgc agattgcggc ggaaagtcct ggtgatcttt ttgatggcac agagattgat
     4981 gaaatcctta cgttgagaat actaacactg actgatgaag agaaacgtga gattcgggca
     5041 agcgatgatc gcgcacgacg aattctcgaa agaactgaga tgcttccgct ggaacaattc
     5101 atgaaacttc atggagcgat gaaaggcgtc aatcgaaagt agcatgaacg aatgggagtg
     5161 gcagttgctt gaagagaagc agccgctgga tcaaattttg gtcaacggag tatcggtgaa
     5221 gtgtggcgat cgtgtgatcc tgaggccaaa aggtcgaagc gacattttgg acctggtgtt
     5281 agttggcaag attgccgtta tcgaggcaat cgaacaggat tatgaggggc aggcacacgt
     5341 cgcggtcgtt attgaggatg accctggtcg agattttgga gtaatgcggc aaccaggtca
     5401 ccgatttttc ttctcgactg ctgaagtcga accattggtg gagtcaaggt cgacggagaa
     5461 tttatgactt cgcgagttct tgtcgccggc atcggcaata tctttatggg agacgacgcg
     5521 tttggttgtg aggtgattcg caggctcgcc ggatttcagt ttcctgagaa cgtccgaatc
     5581 ttcgactttg gcattcgtgg gttcgatttg gcttacgcac tgatggaagg ctacgacttg
     5641 acgattctcg tagacgcggt accacgcggg gaaccggccg gaacggttta caccatcgag
     5701 ccggaccttc ggcaattgga gcaaccagac caggcaaatc tcgttgtgga gacccatggc
     5761 atgaatccca tgaaggtttt gcagctggtg aactcgatgg gaggcacgtt caaaaagatc
     5821 ttgttggtgg gttgcgagcc ggaatcgttt ggaccagaag aaggacaaat ggggctaagt
     5881 ccgtcagtgg ccgcatctgt ggatcgtgcc gtgcaggtaa ttaatgatct gatttccgaa
     5941 tttcttggaa atccagcact ttgggaggcg aacaaaaatg ggcaggaaca atgggtctca
     6001 gaaagagtgt aagcattttg gacatcgtgt ttggaaaata gtcggaggac tggctctagc
     6061 ggtcgtggcg gcgggcgtcg tggcgactct tccagacatt aaacgttaca tccgcatctc
     6121 gcgaatgtaa gccccgcgcc ttcgtctaac catcggcatt ggtggatgct tacttagaag
     6181 ttcttaaccc ccgaagcaca aggagaagtg tcatgactaa caatggttcg gaatcgaagg
     6241 acatattgta tttggttggc ggttttgcgc tgctggtcgc gggagccgga ttgatcatgg
     6301 cccatcccga gattcgtaag caggtacgcg ctgggctgga cagggttatc cccggtttac
     6361 caggtaattt gggttcgagt ttgtcgacag tggtccccga tgtgcaacgt tatttgaaga
     6421 ttaggtcaat gtgaagaaat gaaaaatgag ggaagtttag ttaaaggggt tgcggcaggt
     6481 atggctggag gtttagttgc ctcagcagta atgaatcagt ttcaaagtgc gcttagtaaa
     6541 cttatgagcg gcgatgaacg gtctcatgga gctcaatcac aacaaccggg ctctccagag
     6601 catggaatcg gcaaagcttt gcaagagcgt ggtagtgatg atccagacga caatgcagcc
     6661 gtgagagcgg ctaatgcggt tgaggaattt gtctttaacg aaagactaac caaaacggaa
     6721 aaagagacgg ccggaacagt cgctcactat gcaatgggtg cagcatccgg cgcgatctat
     6781 ggagcctgcg ccgagttggc gcctggggtt tctataggtg agggtgtccc gttcggcgca
     6841 gcggtttggc tgatcgctga cgaggcgatt gttcccgcca taggattatc caaatcagca
     6901 aaaaattatc cgttgtcaac gcacgcatat gcgctggctt ctcatctggt ctacggcctg
     6961 acaaccgagt tggtacgtaa agctgttcgg aaagcgttat agacaaatat ggcaactgaa
     7021 ctcgtgagcg cacttgcgca gtatcgaatt ctcccggagg agagcacatt tacagtgcag
     7081 gctttcgccg aaggtctgtt ctcggcgttt ggacatgacc cggtaatcgc cgttcgcagt
     7141 ttcaacggca acgccgagtt catgcctgag acgtttgaga aagcggcgct ccatctctcg
     7201 gtcgatgcgt catcgttggt ggtcgcgaat gaggttaaag agaaagatcg actagagatt
     7261 gaaaacacaa tgcggcggga ggtgctcgag gttcaaactt atccggagat taagtttgac
     7321 agcaacaatg tagctctcaa ccgcttagga ccgggtcgct atcgtgcgcg gctgatcgga
     7381 gatctgagtc tacacggcgt tacgcaaaag aatgtttgga taactgccga ggtcaccttg
     7441 tcggcagaaa tgttgcgggc taaaggcgag tatcagcttc gccagacaga tttcaaggtt
     7501 aaacccgttt ccgtagccgg cggcacactg aaaatcaaga atgaattgaa atgcgcattt
     7561 gatatttgcg caaaacccgc gtagcaatgc acgaactttc aattgcaatg agcatggtcg
     7621 aaatggccac ggaggagtgt caacgactcg gagccgagcg cgtgaccgct cttcatttaa
     7681 aggttggact gttgtctgga gtcgttaagg aagcactaat cttctcgtat gaagttgtgt
     7741 gcgccggcac accattgcaa ggttcaaaac ttgtaattga agacgtgccg gtaaaggttt
     7801 attgcccgcg ctgcaattcc aaaaaagacc tcgactcgat acaactgttt gcttgtccag
     7861 cttgcggaga gttgacaccc gaagtccttg agggaagaga actgcaggtc gttgcgatgg
     7921 aaatcgaaga aaggcaagaa gttgccgagg cagtgatggc atgagtacac cccgactcgt
     7981 cgaagtcagg cagaacgtct tgaagcagaa tgatctgctg gcgcgttcac tcagaaagcg
     8041 attcgcggac gccggcgttt cggtactgag tcttgtatcg acgcctggtg caggcaaaac
     8101 tgcgttactt gaacacacac taagcagtct gcgcgaaacg ggtttgcaag tggcggcact
     8161 ggttggcgat ttggcgaccg acaatgacgc cgagcggcta gcccgcagcc aggttcctgt
     8221 tcgccagatt acgactggta cagtttgcca tcttgatgcc agcatggttg aaaaagcgct
     8281 tggagattgg gacgtcgaga aactcgatat tttgtttatt gagaatgtcg gcaacatggt
     8341 ctgtccttca agttatgacc tcggagagaa tctgcggata gttttgctct ctacgaccga
     8401 aggcgaagac aaaccattga agtatccgac gatcttcaat acgtcggaca ttgccgtcat
     8461 aacaaagatg gacctggctt cggtagttgg ctttgacttg gaggcagcga gaacaaacat
     8521 acagcgagtg cggccgggaa tgaaaactat ggaagtgtcg gttaaaaccg gcgaaggcat
     8581 aatcgaattt ctggacctga ttgtttccca t
//
LOCUS       DASSFP010000229         4634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1548414, whole genome shotgun sequence.
ACCESSION   DASSFP010000229 DASSFP010000000
VERSION     DASSFP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548414"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..800
                     /locus_tag="VFH91_05020"
     CDS             189..800
                     /locus_tag="VFH91_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862381.1"
                     /translation="MRKFVLALACILSLLLHSALTKQGVFVVSQVSGAQVVKHGGKIE
                     SKYDGFTHETSMRLRKMKVECSAFRDSFDYKNYCVSIDVTLHCPGVQLNFVRDVSLQV
                     IFDTKDWGLRHPVDQRELSVVADGETFRLGKMQLITQKDEPMRENMVETLETTIPYKV
                     FKKMAGAETVQVQVGKSNVEFRDKNILALRDLDSRVLTSAQAQ"
     gene            complement(794..2023)
                     /locus_tag="VFH91_05025"
     CDS             complement(794..2023)
                     /locus_tag="VFH91_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6862382.1"
                     /translation="MEHIVLEVGLALALIAIAALISARVRFSVVPLLILAGMVVGPHA
                     PKIGPFDFGFISSAPLIEFMGRVGVLFLLFYLGLEFSVSRLIKSGRSIVIGGSIYIAI
                     NLVAGMAYAALLGWPFKEVLVATGITTISSSAIVAKVLFDLKRTANPETEMILGIIMF
                     EDIFLAVYLSLVSGIVLSGATSLSGIAKSAGLALGFILGIIVLGRLATPLLNRIFRIS
                     SNEVFLLVLFACLFLLAGLGETIHVAEAIGALLLGLVLAETEHSERIEHLIVPFRDFF
                     GALFFFGFGLTIDPFALGGAVWPVLGAVVVTLFGNIIAGMLAGRTAGLGAGPSLNVGL
                     TIVSRGEFSIIMANLAKTGGLLPVLQPFAALYVLILAVLGPLLTRESERIFDYALAAV
                     RKLRKPVQAESSEPADY"
     gene            complement(2025..2525)
                     /locus_tag="VFH91_05030"
     CDS             complement(2025..2525)
                     /locus_tag="VFH91_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HET6862383.1"
                     /translation="MATISESNLPGVGRKFQVETLSGDRLTIVIHDDGTRELYHFTRK
                     NLDRAASVLTLSDGEARQIAGIVGGLTYVPKALPSTEIVLEDLVLEWFTIEKGAAATG
                     RTISELQVRTRTGASIVSLIEPNRSTRTNPEAGTLLNEGATLILAGDRLSIERLKRLL
                     VSGKGD"
     gene            complement(3170..4054)
                     /locus_tag="VFH91_05035"
     CDS             complement(3170..4054)
                     /locus_tag="VFH91_05035"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET6862384.1"
                     /translation="MNNNTQVRSVSGIINSMETLEGEGFLVHRPFPTYALSDFDPFLL
                     LDEMGPKDLAPGEAKGAPDHPHRGFETVTYMLEGRMQHKDSRGNAGKLTPGDVQWMTA
                     GSGVVHSEMPELDFAKNGGRLHGFQLWVNLPKRNKMTNPRYQEIPSAKIPVAASEDGL
                     VSVRVIAGESLGKKAVIETHTPIMYLHFTVQPGGRVVQPVPSEYNTFAYVITGEGTFG
                     PELERASRGQMVMFASDGEGISIENKGSLPLDVLVIGGVPLKETVARYGPFVMNTREE
                     ILEAFEDFRSGRMGEIAF"
     gene            4188..4574
                     /locus_tag="VFH91_05040"
     CDS             4188..4574
                     /locus_tag="VFH91_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6862385.1"
                     /translation="MAVKSATTKRARDKVDNGKPHQVCARFHHAVELIGRRWTGAIIS
                     SMLVRPQCFNEFLAAIPGLSDRLLTERLRELEAEGLCSRTVIAGPPVRVSYELTESGR
                     DLEPAIEALWPHQHRTDDRSGPTATN"
BASE COUNT         1201 a         1155 c         1183 g         1095 t
ORIGIN      
        1 aaacggtggg gtcattgcca taaaatgcca ggctgcggcg ctggaaaatg ttttatttga
       61 cacgttcgcc gccgcttcgc tattttgttg ttagctcttt gttggctggc tgcggcgttc
      121 gcagtttctt gctcaaacct ctcacacaat ctcaactcat ccccgaacga aaacacagga
      181 gaactacaat gcgcaaattt gttctcgctt tagcgtgtat cctctcgctg cttctgcact
      241 ctgctttgac gaaacagggg gtttttgtcg tgtcgcaggt gagtggggcg caagttgtca
      301 aacatggtgg caagattgaa tcgaaatatg acggctttac tcacgaaacg tcgatgcgtt
      361 tgcgaaagat gaaagttgag tgttcagcgt tccgagatag cttcgattac aaaaactatt
      421 gtgtgagcat tgatgtgaca cttcactgtc cgggagtgca gttgaacttc gtacgcgacg
      481 tttcgcttca ggtgattttt gatacaaagg attggggctt aagacatccc gtcgatcaga
      541 gagaattgtc tgtggttgcc gacggcgaaa cctttcgtct cgggaagatg caactgataa
      601 ctcaaaaaga tgaacccatg cgagagaaca tggttgagac gctcgaaacc acgattcctt
      661 acaaagtctt taaaaagatg gcgggcgccg aaaccgttca ggttcaggtt ggcaagagca
      721 atgttgagtt tcgcgataag aatatcttag cgttacgtga tctcgacagt cgcgttttga
      781 cttcagcgca agctcaataa tccgcgggtt ccgaagattc tgcctgtacc ggcttccgta
      841 attttcgtac ggcagctaac gcatagtcga aaattctttc cgattccctg gtcagtaacg
      901 gtccaagcac ggcaagtatc aggacatata gcgctgcaaa gggttgcaag accggcagta
      961 ggcctccggt tttcgcgagg ttggccataa tgatcgagaa ttcgccgcgc gaaacaatcg
     1021 tcaggcccac gttaagagat ggaccagctc cgagtccggc agttctgcct gcaagcatcc
     1081 cggcgataat gttgccgaaa agcgtaacga ctacagcgcc taataccggc cagactgcgc
     1141 cgccaagcgc aaacggatcg atcgtcaaac caaaaccaaa gaaaaacagg gcaccaaaga
     1201 aatctcgaaa ggggacaatc aaatgttcga tcctttcgct atgctctgtt tccgccagga
     1261 ctaaaccaag cagaagtgct ccgatagctt ctgcgacgtg aatagtctct cccaatccgg
     1321 cgagcagaaa aagacaagca aataggacca gcagaaaaac ttcattggaa gagatgcgaa
     1381 agatccgatt gagtaaaggc gttgccaatc gtcccagaac aataatccca agaataaaac
     1441 cgagagcaag cccggcagac tttgcgattc ctgataacga cgtggcgccg ctaagaacta
     1501 tgcctgacac cagcgataaa tagacagcaa gaaaaatatc ctcaaacatg atgatgccga
     1561 ggatcatttc agtctccgga ttagcagtcc gtttcagatc aaagagaact ttcgcgacga
     1621 tcgcgctgga ggaaatggtt gtgataccgg tagcgaccag gacctcttta aaaggccagc
     1681 ctagtaacgc tgcataggcc attccggcaa cgagattaat tgcaatatag attgatcctc
     1741 cgatgacgat cgagcggccg gatttaataa gcctgctgac cgaaaattcc aggcctagat
     1801 aaaagagcag gaacaaaact cctacccttc ccataaactc gattagaggc gcactcgaaa
     1861 taaaaccgaa gtcgaatggt ccaatcttcg gcgcatgcgg gccaaccacc attcccgcaa
     1921 ggattagtag cgggacgaca gaaaatcgta cgcgggccga aatgagggca gctatggcga
     1981 taagggcaag cgcgaggcca acttccagga cgatatgttc catactaatc gccctttcct
     2041 gacacgagca gccgcttcaa acgctcgatg cttaaacggt caccagccag gatcaacgtg
     2101 gcgccttcat tcagtagtgt gcctgcttca ggattggtgc gcgtggagcg attcggttcg
     2161 ataagagaaa cgatcgacgc gccggtgcgt gttctgacct gtaactcgct aattgtgcga
     2221 ccggtggcag ctgcgccttt ctcaatcgta aaccattcga gtacgagatc ttccaacacg
     2281 atttccgtcg acggcagggc cttgggtaca taagtcaatc cacccacgat gcctgcgatc
     2341 tgtcgtgctt caccgtcgct gagagtcaat acggaagccg ctcgatcgag attcttgcga
     2401 gtgaagtgat atagctcgcg tgtaccatcg tcatgaatga ctatcgttag gcgatcgccg
     2461 gacaacgtct cgacctgaaa ctttcgaccc acgccgggaa gatttgattc gctaatggta
     2521 gccataccat tctcctttgc ttaaagcgtt gagtgccatt cgcaccaatt caagttcgaa
     2581 tggagagctt ccaggcgagc ttaagtcatt cgcttgagtg actaactcgc aaaccaggac
     2641 ttcaattgtt aggacgtctt aaagagttag tgggggagct cgccctttcg tgggcgaata
     2701 gaaagcggag ctaaaacagg gcagagcagt attaagacac tcaaagatcc ttagatgcga
     2761 atccaggttc gcaacaactg gcggaatttc gacgggtgag ctttggcgct tctcactttt
     2821 gcgaagtgac gtcgtttcca tcgggttagt gtgatgagca ttgggagaag tatttaactc
     2881 cgcccgggcg atggcagccg ttgactcgta agctgaggga aagcttgtga aaggtcttaa
     2941 ttgtaagcct tcgccgattg agaacaagag actcgcaacg accagacaca ggacaagtcg
     3001 gagccaaagt tcaatcttta ctggtggcag tcgagtcatt gtttcttgta ccgctgagct
     3061 tgtggtcgac gaccatccta gcagccgaac tccaatctcg taaaatgaag gattagagtt
     3121 cggcttctga ctacgaagga ggctgagtct gcgaggatct tacggttaat caaaaggcga
     3181 tttctcccat ccggccgctg cgaaagtctt cgaatgcctc aaggatctct tcccgcgtgt
     3241 tcatgacaaa aggtccataa cgcgctacgg tttccttcag tggcacccca ccaattacca
     3301 acacgtcaag tgggagtgaa cctttgtttt cgatcgatat gccttcgcca tcagatgcaa
     3361 acatcaccat ctgacctctg gatgctcgct cgagctctgg tccaaaagtt ccttcgcccg
     3421 ttatcacgta tgcaaaggtg ttgtactctg aaggaacagg ctgaactacg cgcccacctg
     3481 gttgaacagt gaaatgcaag tacatgattg gtgtatgagt ctcaattacg gctttctttc
     3541 caagagactc accagcaatg acgcgaacac taaccagacc atcttcagaa gctgccactg
     3601 gtatctttgc cgaggggatt tcctgatatc tcggattcgt catcttgttt cgtttaggta
     3661 gattcaccca aagttgaaag ccgtgtagcc ggccgccatt ttttgcaaaa tcgagctccg
     3721 gcatttccga atgaaccacc ccggatcccg cagtcatcca ctgtacatca ccgggcgtta
     3781 acttcccggc gttgccacgt gaatctttat gctgcattcg accttcgagc atgtatgtga
     3841 cagtctcgaa acccctgtgt ggatgatcgg gcgcaccttt agcttcacca ggcgccagat
     3901 cttttggccc catctcatcc aggagcagga acggatcgaa gtctgacagg gcgtaggttg
     3961 ggaaaggcct gtggaccaga aagccctcgc cttccagtgt ctccatactg ttaatgatgc
     4021 cggagacgga tctaacttgt gtattgttat tcatctggaa aaactccgta gcaaaaatct
     4081 cctcacgttg cataattaac ctacttttag taagctgagc ctcagaataa gacggtagcc
     4141 tacgtttgtc aagtaggcaa cgaaaagtaa gtttgaggtg aggtgtagtg gcagtaaaga
     4201 gtgcaacaac aaagcgagcg cgtgacaaag ttgataatgg aaagccgcat caggtgtgtg
     4261 cgcggtttca ccatgcggtt gagctaattg gtcgccgttg gaccggagcg atcatcagtt
     4321 cgatgctggt gcggccacag tgcttcaatg agtttctggc tgcgattccc ggtctatcgg
     4381 atcgattatt gaccgaacgt ctacgcgagc tggaggctga gggtctttgt tcgcgaacag
     4441 taatcgccgg cccgccggtt cgagttagtt atgagttgac ggagtcggga cgtgaccttg
     4501 agccggccat tgaagcactg tggccgcacc agcatcgaac tgatgatcgc tccggtccaa
     4561 cggcgaccaa ttagctcaac cgcatggtga aaccgcgcac acacctgatg cggctttcca
     4621 ttatcaactt tgtc
//
LOCUS       DASSFP010000230         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_958529, whole genome shotgun sequence.
ACCESSION   DASSFP010000230 DASSFP010000000
VERSION     DASSFP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958529"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFH91_05045"
     CDS             complement(<1..197)
                     /locus_tag="VFH91_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862386.1"
                     /translation="MGAFRKLSQLLIILAVLFQVALSAPARKWTEAQANNWYRNQPWL
                     VGSNYIPATAINELEMWQADT"
     gene            complement(293..994)
                     /locus_tag="VFH91_05050"
     CDS             complement(293..994)
                     /locus_tag="VFH91_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6862387.1"
                     /translation="MNDKPRILVVDDESQLTRVLRTGLTAHGYDVRTASDGVAGFRQF
                     TDWRPDLIITDLAMPNMDGLELCRKLRSVSQVPVIVLSAKGEEKIKVEALDLGADDFI
                     TKPFGIDELLARVRAALRRSSVQSLTATDEAAFEAGDFQVDLQDHSVKVSGKEIRLTP
                     KEFDLLVYFTKHPGKVLTHRTLLAAVWGGNYVEQNEYLRVFVGNLRKKIERDPAAPKY
                     ILTEPWIGYRFDPGS"
     gene            complement(991..2208)
                     /locus_tag="VFH91_05055"
     CDS             complement(991..2208)
                     /locus_tag="VFH91_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET6862388.1"
                     /translation="MPRKPFDQAWTDYLVAVFSVAALTIVLKMIGAHVNSTTVALVFL
                     LNVLCIATWRGSFPALVTSIIAVLCFNYFFLPPFGTFNIASADNWVALAAFLLTALTA
                     GQLSARAKKRAEEAEAGKSQIEKLYGQLRDAFERASQAEALRQSEKLKSALLDAVTHD
                     LRTPLTSIKASITTLLDDQRGRADEEQAFAIDSDSKLEMMEVIDQETDRLNRFISGLI
                     ELARIEAGQLQLRRRWGAVDEIIANALSRADPITLHHRIELGIERELPVVRVDERAVS
                     EVVYTLVDNAAKYSPTESCIRITAKRSPHDQIRMEVENEGETVPPALRERVFDKFFRA
                     TRDGDISTHKPSGTGMGLAIAKGIVEAHGGRIWIEAGTDGKGTRVVFTLPIGDEDPSE
                     VIAPANVESAREL"
     gene            2462..5521
                     /locus_tag="VFH91_05060"
     CDS             2462..5521
                     /locus_tag="VFH91_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6862389.1"
                     /translation="MGIATGAQVGRYQVRNLIGSGGMGEVYIARDIKLDRTVALKILA
                     GEIASDPNRIHRFVQEARAASALNHPNILTIYEIEQTDDAYYIATEFIDGVTLREHMT
                     RTNLSVSEALDIASQIASALAAAHEVGIVHRDIKPENIMLRPDGLVKILDFGIAKLST
                     TESGTVVDADAETRALVNTQPGLLMGTVRYMSPEQSRGSAVDSRSDIWSLTVVLYEML
                     TGELPFNGPTNADIIAAVIKTPTPSIAVHRIDTPPELQQIISKALEKNPNDRYQTAKD
                     LVVDLRRLQKRIEFEGFEIGQSTNYLSKAPRDKCLTNEQSLPPNNLVGPRSSVIGRAR
                     EIDEICTRLREGTRLVTLTGVGGTGKTTLAQAVAHKMLGEFVDGVFFIKLGAITQPEL
                     VAASIAQPLGVKEADRRILVESLKDYLRPRKVLLVADNFEQVITARLLIAELLESAPE
                     LKFLLTSRELLHLSWEYEYMVPPLALPESSTAVTAAQSNKYDAVILFVERARALKPSF
                     TVTDENASSVLEICKKLDGLPLAIELAAARIKILSPQAILAKLDSRLQFLTGGAHDLP
                     TRQRTMRGAVDWSYELLSDEEKRLFRRLAVFAGGFTINAATSVMVSSSSQDSSIDVID
                     GITSLVDKSLLVTREQADGEVRFRMLGVVREYALELLDVSTEADSTRRAHATYFLSIA
                     EEAEPHLQGWQPAKWLNRLEEEHDNLRAALRWSVVHQVETAGRLAAAIRYFWNFQGYL
                     TEGLRWSEAVLNLGQEIPRAARWKILSMAGNLARFLGNHQTARDMYEEGLAEGRAIND
                     LSQISLSCRGLSGLAMEQEDFGSARAFLDEALAAARQANDQFGVARSLSMMGDLARTR
                     GDDLQARPLYEEALAICRRLGNQYAIGNILNNLAAAEYVLEDYETSSLHFAEALKMAQ
                     ASGGKIAGDKIAISYALDGFGALAVARNKLDVATQLAAAAQRLRESINYNIEPAERRF
                     RDAYVSSAQSKLTETDFSTAYSNGRRLKLGQALSLALLECGGWSPQS"
BASE COUNT         1426 a         1474 c         1402 g         1386 t
ORIGIN      
        1 aaagtatcgg cctgccacat ctcgagttcg ttaatcgcgg tcgcggggat gtagttgctg
       61 ccaaccagcc acggctgatt ccgataccag ttatttgctt gcgcttcggt ccatttcctt
      121 gcgggcgctg acaacgctac ctggaacagg accgccagaa tgattagtaa ctgtgacagt
      181 tttcgaaaag ctcccatcag ttatttcctc tctcacctgc attgttgagt tctgcgcaaa
      241 gctatgaaga gtgcttgaat tagcaaatca atcgggactg acacgaacaa gttcaactgc
      301 ccggatcgaa gcgatagccg atccagggtt cggtcaaaat gtacttcggt gccgccggat
      361 cgcgttcaat cttctttctc aaatttccga caaacacacg aagatattca ttttgttcga
      421 cataatttcc tccccacacc gcagccaata acgtgcggtg agtcagaacc tttccgggat
      481 gcttcgtgaa gtagaccaac aggtcgaact ctttgggcgt caggcggatc tccttaccgc
      541 taactttcac ggaatgatct tgaagatcca cttgaaagtc accagcttca aacgctgctt
      601 cgtccgtagc ggtgagagat tgaacactcg agcgacgtaa agcagctcgc accctcgcga
      661 gcagttcatc gataccaaac ggcttggtta taaaatcatc cgcaccaagg tccagggcct
      721 caaccttgat cttctcttca cctttcgctg ataagactat gacgggaact tgcgagacag
      781 aacgcagttt gcgacagagt tcgaggccat ccatattcgg catcgcgagg tccgtgatta
      841 tcaagtctgg ccgccagtcc gtaaactgtc gaaaaccagc tacaccatcg gaagctgtgc
      901 gcacgtcgta accgtgcgca gtcaaacctg tccgcaatac acgcgtcagt tgtgactcat
      961 catcaactac taaaatacgc ggcttatcgt tcataactcg cgtgcagatt ccacatttgc
     1021 aggtgctatc acttcagacg ggtcttcatc acctatcggc aacgtaaaca ctacgcgcgt
     1081 gccctttcca tccgtgcccg cctcgatcca aatcctgcca ccatgagcct cgacaatccc
     1141 tttcgcgatt gctaatccca taccggtgcc tgacggcttg tgtgttgaaa tatcgccatc
     1201 tctggtggct ctaaaaaact tgtcgaagac tcgctctcgc aaagccggcg gaacagtttc
     1261 accttcattt tcaacttcca ttcgaatctg atcgtgcggt gaccgtttcg cagtgatcct
     1321 tatacaagat tcagttgggg aatactttgc agcgttgtcg actaacgtat aaacgacttc
     1381 gcttacagcc cgttcatcaa ctcttactac cggcaactcc ctttcaatcc caagctcaat
     1441 ccgatgatga agcgtaatcg gatcagcgcg cgacaaagcg ttggcaatga tctcgtcgac
     1501 agccccccat cgtctgcgaa gctgtagttg ccctgcctcg atacgagcca actcgattaa
     1561 gccacttatg aaacggttta gtcgatcagt ttcctggtct ataacctcca tcatttcgag
     1621 ttttgagtca gagtcaatgg cgaatgcctg ttcttcatca gccctgcccc tttggtcatc
     1681 cagcagagta gttatcgatg ctttaatcga ggtgagcggc gttctaagat cgtgtgttac
     1741 tgcatccaac aacgcggact tcagtttttc actttgacgt aaagcttcgg cctggctcgc
     1801 gcgttcgaag gcatcacgca attgaccata gagtttttcg atttgactct tgccggcctc
     1861 agcttcctcg gcacgctttt tcgcccgcgc agaaagttgg cccgcggtaa gcgctgttaa
     1921 gagaaaggct gcgagggcga cccagttatc agcggaggca atattgaacg tgccaaacgg
     1981 gggtaaaaaa aagtagttga aacaaaggac ggcgatgatt gaagttacga gcgcgggaaa
     2041 gctgccccgc catgtagcaa tacataaaac gttgagtaaa aaaacgagag ccaccgtagt
     2101 cgagttgaca tgcgcgccta tcattttcag cacgattgtt aacgcagcga cgctaaacac
     2161 cgcgactaaa tagtcggtcc atgcttgatc gaacggtttt ctgggcattt ttcgtattgt
     2221 atgacacctg accgctcatt ctcactcaga agaggcgtgt ccaaggtttt gattctaacc
     2281 atggaggtgg atccaaatca tagagtttag atttaggacg ctacttcccc gtcactacag
     2341 ttgacacccg gaagaagttg caacaatctc taacgagtca gattcctctt atatcaatgc
     2401 gaagttgcac gccccaacac ttaccacgtc cagttccatc caaaccggtt attaagtaat
     2461 catgggtatt gccacaggag cacaagtcgg acgctaccag gtgcgaaacc tgattggttc
     2521 aggcggaatg ggcgaagtct atattgcgcg cgatatcaaa ttggaccgta ccgtcgcgtt
     2581 gaagatattg gccggagaga ttgcgtctga cccaaatcgg attcatcgct ttgtacaaga
     2641 agcgagagca gcctcagcac tgaatcatcc aaacatactg accatttatg agatcgagca
     2701 gaccgatgat gcttactaca tcgcgaccga gttcatcgac ggagtaactc tacgcgagca
     2761 tatgacccgt acaaacttgt cagtttcgga agctctggat atcgcttcgc aaatcgcctc
     2821 agcactggca gcggcacatg aggtcggaat cgttcatcga gacatcaagc ctgaaaacat
     2881 catgttgcga cctgatggtc tggtaaaaat cctggacttc ggcatcgcaa aactttcaac
     2941 gactgaatcc ggcactgtcg ttgatgcgga tgcggagaca agggcgctcg tgaacactca
     3001 accggggttg ttgatgggga cagttcgcta catgtcgccc gaacagtcga gaggatcagc
     3061 cgtcgattcg cggtcggaca tctggagtct gacagtcgtg ctctacgaaa tgcttaccgg
     3121 tgaacttcca ttcaatggtc caacaaacgc cgatattatt gctgcggtta ttaagactcc
     3181 tactccgtct attgctgttc atcgaataga tacaccaccg gaactgcaac agatcatttc
     3241 aaaagcctta gagaagaatc caaatgatcg ataccaaact gcaaaagacc tggttgtcga
     3301 tctccggcga ttacaaaagc gaatagaatt tgaaggcttt gaaataggtc agtcgaccaa
     3361 ctatttatca aaagcaccac gtgataagtg tctgacaaac gaacagagcc ttcctccaaa
     3421 caacctggtt ggtccacgtt catcggtcat tggtcgcgct cgcgaaattg acgaaatctg
     3481 tacgcggctc agagaaggta ctcgccttgt gactctgacg ggggttggtg gcaccggaaa
     3541 aacaaccctc gcacaggcag tcgcccataa aatgttgggg gagtttgtgg atggcgtctt
     3601 ctttattaag cttggcgcta tcactcaacc tgaacttgta gctgcctcga ttgcgcagcc
     3661 tttgggggta aaagaggcgg accgtcgcat tctggtcgaa agtcttaaag actatttgag
     3721 gcccagaaag gtcttgttag tcgcagacaa tttcgaacaa gttatcactg cacgtttgct
     3781 tatagccgaa ttactggaat cggcacctga actgaagttt cttctcacca gccgcgaact
     3841 actacacttg agttgggaat acgagtacat ggtgccgccg ctcgcgttgc cggagtcatc
     3901 aactgctgtg acggcagcgc agtcaaacaa atacgatgca gtcattttgt ttgttgagag
     3961 ggctcgcgcg ctcaagccga gctttactgt gactgacgaa aacgcttcca gtgtgctcga
     4021 gatttgtaag aagctcgacg gactgccact cgcaatcgaa ctggccgcgg caaggattaa
     4081 gattctttct ccccaggcaa ttcttgcgaa actagatagc cggctacagt ttcttacggg
     4141 cggcgcgcat gaccttccaa ctcgacaacg aacaatgcgc ggtgcagtcg attggagtta
     4201 cgagctcctg agcgatgagg aaaagcgact atttcgcagg ctggcagtat ttgcaggtgg
     4261 gttcaccatc aacgctgcca catcggtgat ggtgtcatcc agctctcaag attcgagcat
     4321 tgacgtaatt gacggaatca cttcattagt tgacaagagt ttgcttgtta caagggaaca
     4381 agcagacggt gaagttcgct ttcgaatgct gggcgttgtt cgtgaatatg cgctcgaact
     4441 cctggatgtc agcactgaag ctgactctac gcgtcgcgct catgcgacat actttctgtc
     4501 tatcgctgaa gaggctgaac cgcacctgca aggatggcaa ccagcaaaat ggctcaacag
     4561 gctcgaggaa gaacacgaca acctgcgagc tgcgctgcgc tggtcggtgg tccatcaagt
     4621 cgagaccgcc ggacgcctcg cagcggcgat acgatacttc tggaattttc agggttatct
     4681 aacagaaggt ctgcggtggt ccgaggcagt gttgaatctt ggacaggaga taccaagagc
     4741 agcacgatgg aagatattga gtatggcggg aaatctcgcg cgcttcttgg ggaatcatca
     4801 aacagcgcgc gatatgtacg aagaaggatt agccgaaggt cgagccataa atgatttatc
     4861 gcagatctct ttatcgtgca gaggtctttc gggattggcg atggaacagg aagatttcgg
     4921 ttctgcgcga gctttcctcg acgaggcttt ggccgcagct cgtcaggcaa acgatcagtt
     4981 tggagttgct cgctcgctca gcatgatggg agatcttgct cgaacgcgag gagatgacct
     5041 ccaggcccgt cctttgtacg aagaggccct ggctatttgc cggagacttg gtaatcaata
     5101 cgcgatcggt aacattttga ataacctcgc cgccgcagaa tacgttctgg aggactacga
     5161 aacctcgtca cttcactttg cagaggcatt aaagatggcc caggcttcag gcggcaaaat
     5221 tgccggcgac aagatcgcga tctcatacgc actcgacggt ttcggagctc tcgcagttgc
     5281 tcgcaataaa ctagacgtgg caactcaact ggcggccgcc gcgcaacggt tacgtgagtc
     5341 cattaactac aacattgagc cagcggagcg ccgcttcagg gatgcatatg tttcatcggc
     5401 ccaatctaaa ctcactgaaa cagatttttc tacggcctac tccaacgggc gacgtctcaa
     5461 gttaggccag gcgctctcgc tcgcactttt ggagtgcgga ggctggtcac ctcaatcgta
     5521 aatctctcgt tatgcggagt tgtcggcgat ttctgacttg ttcagtgcct gttctttata
     5581 tataaagcct cagcaaccag ttgtctcaaa tccaactttg gaaataatca tgagaacctt
     5641 aatggtttta ctcatcctcg cggccacctc agccttaact cttgcggt
//
LOCUS       DASSFP010000231         5102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1100520, whole genome shotgun sequence.
ACCESSION   DASSFP010000231 DASSFP010000000
VERSION     DASSFP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100520"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="larC"
                     /locus_tag="VFH91_05065"
     CDS             <1..348
                     /gene="larC"
                     /locus_tag="VFH91_05065"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HET6862390.1"
                     /translation="DCFFTPVQMKKNRPGVLLSILSDARTRESIMNLLFTETTTLGVR
                     SYEVLRRALQRGVVAVETQYGRIDVKVARLNGRVVNEMPEFEQCREAARSAKVPLKTV
                     EDAVKLALAKNRG"
     gene            366..980
                     /gene="folE"
                     /locus_tag="VFH91_05070"
     CDS             366..980
                     /gene="folE"
                     /locus_tag="VFH91_05070"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET6862391.1"
                     /translation="MATKFPETKDGSREIPAVDLELIAQGVRLILEGIGEDPERVGLQ
                     ETPRRVAEMYSELTSGMREDPRQHVIPLPGDTHDEMVIVKDISIASLCEHHLAPFVGK
                     CHIAYIPKEGRILGISKLARLAETFSRRLQLQERLTTDIANTLFQGLKPLGVMVVIEA
                     EHTCMTLRGVRKADAKTVTSAVLGGFRSDPRTRAEAMALIKGRD"
     gene            992..2731
                     /locus_tag="VFH91_05075"
     CDS             992..2731
                     /locus_tag="VFH91_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6862392.1"
                     /translation="MSLHLKLILVGALTLFFLSGSVVLSQRASSPPSAINEATLKAHI
                     RFLSDDLLEGRGTGARGGELAAKYIASQLESVGIKGAEKNGSFFQPVSLVGVKADPNT
                     TLMVTADATSNPGTVAGANTKLTEIFNFGDDYVAFTGAQKERVTVNTDLVFVGYGISA
                     PEQRWNDYKGQPDDYRGKVLVMLVNDPPATTEEPDLFGGKALTYYGRWTYKYEEAARR
                     GAAGVILLHTDQSAGYPWSVVRTSNGSWRFDIERTAGDQTPFLQFRSWMTDDAAKRLM
                     KLAGQDLDKLRTEAASRDFKPFNLGIKVKLDLRSEVQRVAAPNVVGMLMGSDPALRDE
                     YVVFSAHWDHLGIGAPNKAGDTIYNGALDNASGVACVLAIAETLARTPLAQRPKRSIL
                     FLFPTAEEQGLLGAEWYTAHPVVPIEKTAANVNLDSMNVLGVTSDFIPLGAERSSLKA
                     VVDAVAKERKLKVSPDARPEQGSFYRSDHFPFAKVGVPSISLKEGNEYVGHTKEWGEE
                     QFKAYNTAHYHQPSDELRSSWDFKGMIQEAQIALAIGRKIADLPQKPEFNPGDEFANK
                     RRGSGSSIKTPTQ"
     gene            2758..4872
                     /gene="metG"
                     /locus_tag="VFH91_05080"
     CDS             2758..4872
                     /gene="metG"
                     /locus_tag="VFH91_05080"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET6862393.1"
                     /translation="METFYVTTPIYYANSFPHLGHLYTMIIGDAIARSKRQRGIDTYF
                     LTGTDEHGINIERAAQENNRTPKEQADYVVRYYKKMTAEFGLDHEHGGYDIFMRTTAP
                     FHYEGVSELWRRLAGSKTPKGNDALYKGHYEGWFCAPCAAYKTEDEYAKPKIEGEPPT
                     CLIHETPLDRVSEESYFFRLSDFDEALLSVYERPDFVRPESRKNEVISFVRGGLQDLS
                     VSRPKSSVSWGVPVPGNPDHTMYVWFEALSNYITAIGFGNEEREKAAGFEKYWPALHL
                     VGKDILRFHAVYWPAFLLAAGVELPRGIVAHGMWLDPNGRKMSKTIGNTIELNLLQQH
                     FPVDAVRYFCLREMVFGQDGRFGYESLIDRTNSDLASGLGNLSSRTLTMIARYCDGHI
                     PSGQISEDKLLLAKRSGVDTDETTVAGFIETARDQYLQHFESFAFSKALETAWSVVSR
                     VDKIITDARPWDLAKDENQKQTLNAVLYRAAETLRWLSVLLYPVMPAAANEVWSQLGL
                     SGSPSKLDPSSLKWGELPEGSVLGEVKPIFPRIDKAKTMAEITEQTKPRGEHGTQADA
                     VDVAEVSGATAPVAPPKDATEAEAVGVTSFIEIDDFSKVDLRVGQVLSAERVPKADKL
                     LLLKVDVNEEQPRQILAGIAQYYEPEKLIGRKVVIVANLKPRKLRGFESQGMVVAASF
                     GPEGRPVIATFTEDVPNGARLK"
     gene            4882..>5102
                     /locus_tag="VFH91_05085"
     CDS             4882..>5102
                     /locus_tag="VFH91_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6862394.1"
                     /translation="MFIDSHAHVDGPEFDQDRLQVLERARLAGVTTILNVGTGDPHSD
                     AFDRAVELGESEEAIYTAIGTHPHDARLY"
BASE COUNT         1273 a         1209 c         1410 g         1210 t
ORIGIN      
        1 gattgtttct ttacgccggt gcagatgaag aaaaatcgtc ccggtgtgct gttgtccatt
       61 ctcagcgatg ctcggacccg ggaatcaatc atgaatctgc tttttaccga gacgacaacc
      121 ttaggcgtac gaagctatga agtgttgaga agagccttgc aacgaggcgt cgtggcggtc
      181 gaaacgcagt atggacgaat cgatgttaaa gtagctcgct tgaacggcag ggtcgtcaac
      241 gagatgcctg agtttgaaca gtgccgtgag gcagcccgaa gcgctaaagt cccgctcaaa
      301 acagtagagg acgctgtcaa actggccctg gctaagaatc gcggttgaac ggtttagaaa
      361 aacgtatggc aacaaagttt cctgaaacga aagacggaag tagagaaata ccggcagtgg
      421 atctcgagct tattgctcaa ggggtccgct tgatactcga gggaatcggc gaggatccag
      481 agcgagttgg attgcaggag actcctcgcc gcgttgccga gatgtattcg gagttaacct
      541 ccgggatgcg agaagatcca cgacaacatg taattccact acccggtgat actcacgacg
      601 aaatggtgat tgtcaaggac atcagcattg cgtctctttg cgagcatcac ctggcgccct
      661 ttgtcgggaa gtgtcacatt gcgtacattc caaaagaagg ccgcatcctt ggaatttcga
      721 agttggcccg tctcgccgaa acattctcca gaagactaca gttgcaagaa cggttgacta
      781 ccgacatcgc gaatacgttg tttcagggat tgaaaccgct cggtgtaatg gtggtgatcg
      841 aagctgaaca tacatgcatg acgctgcggg gagttcgtaa ggcagatgct aagacagtta
      901 cgtccgcagt gttaggtggt tttcgttcag acccacgtac acgcgccgaa gccatggcgc
      961 tcataaaggg cagagattga ggattgcagg tatgtctctt catttgaagt tgatattggt
     1021 tggggctttg accctcttct tcctatctgg ctcagtcgta ctatcccagc gggccagctc
     1081 acctccttcc gcgatcaatg aagcaacatt gaaagctcac attcgatttc tatccgacga
     1141 tctgctggaa gggcgtggta cgggcgcgcg gggaggtgaa cttgccgcga aatacattgc
     1201 gtcgcagtta gaatctgttg gaataaaagg tgctgagaag aatggaagct tttttcagcc
     1261 tgtgtcactg gttggcgtaa aagccgatcc caatacaacc ttgatggtga ctgctgacgc
     1321 aacatccaat cctggtactg ttgcgggtgc gaacactaaa ttgaccgaaa tcttcaattt
     1381 tggcgacgac tatgtcgcat tcaccggcgc gcagaaggag cgagttacag taaatactga
     1441 tctggtcttt gttggttacg gtatttcggc gccggagcaa cggtggaatg actataaagg
     1501 ccagccagat gattatcgtg gcaaagtcct cgtgatgctc gtaaatgatc cgcctgcgac
     1561 gacggaagaa ccagatctgt tcggtggaaa ggcactcacg tattacggcc gttggaccta
     1621 caaatatgaa gaagcggcgc gccggggcgc agcaggtgtg attcttcttc atactgatca
     1681 atcagcaggt tatccctgga gtgttgttag gacctcaaat ggctcatggc gttttgacat
     1741 cgagcgcact gctggagatc aaacaccttt ccttcagttt cgttcgtgga tgactgatga
     1801 tgcggccaaa agattaatga agctggctgg ccaggatctg gacaaactgc gcactgaagc
     1861 agccagtcgt gatttcaaac cattcaatct tggcattaag gtaaagttgg atcttagaag
     1921 tgaagtgcaa cgagttgcag cgccaaacgt tgtcggcatg ttgatgggca gtgatccggc
     1981 gttgcgagac gaatatgtcg tgttctcagc gcattgggac catttgggga ttggtgctcc
     2041 gaacaaagca ggagacacca tttacaacgg tgcactcgac aatgcttcgg gcgtcgcctg
     2101 tgttctagcc attgcggaaa ctctggcgcg tacgccgtta gcgcagcggc caaaacgttc
     2161 gattctcttt ctctttccga cagctgaaga gcaaggttta cttggcgcgg agtggtatac
     2221 agcacatccg gttgtgccga ttgagaagac cgctgctaac gtgaatctgg actcgatgaa
     2281 cgtccttggc gtcacgagtg atttcattcc acttggagcc gaacgttcaa gtttgaaggc
     2341 tgttgttgat gccgttgcga aagagcggaa actgaaggtc tcgcccgatg ctcggcctga
     2401 acaaggatct ttctatcgct ccgatcattt cccgtttgcc aaagttggcg ttccgtcgat
     2461 tagtttgaag gaagggaacg aatacgtcgg ccatacgaag gagtggggcg aggaacagtt
     2521 taaggcctat aacactgcgc actatcatca accatcagac gagctgagat cgtcctggga
     2581 cttcaaaggc atgattcaag aggcacaaat cgcactcgcg ataggccgaa agatcgccga
     2641 cttgccacag aaaccagagt ttaatccagg cgatgaattt gccaacaaac gccgaggttc
     2701 gggatccagt attaagactc cgacgcaatg atcctgaatc gtgactactg agcaaacatg
     2761 gaaactttct acgtaaccac accaatttat tatgcaaaca gttttccgca cctgggtcat
     2821 ctctacacga tgataattgg cgatgcgatt gctcgctcaa agagacagcg cggtatcgac
     2881 acctattttc ttaccggcac ggacgaacac ggaatcaaca tcgaacgtgc ggcccaggaa
     2941 aacaatcgaa ctcccaaaga gcaggctgat tacgtcgtcc gttactacaa gaaaatgacg
     3001 gctgagtttg ggttggacca tgaacatggt ggttacgaca tcttcatgcg tactacagca
     3061 ccgtttcatt atgaaggagt cagcgagtta tggcggcgtt tagcaggctc caagactccc
     3121 aagggaaacg acgcgctcta caagggacac tacgaaggtt ggttctgtgc accgtgtgct
     3181 gcatataaga ctgaagacga gtatgcgaag ccgaagattg aaggtgaacc acccacctgc
     3241 ctgatccacg agactccgct cgatcgagtg tctgaagaaa gctacttctt tcgcctttct
     3301 gattttgatg aagctttgct ttcagtttat gaacgcccgg attttgtgag gcccgagtct
     3361 cgcaaaaacg aagtcattag ctttgttcgt ggaggtctgc aggatctttc ggtgagtcgt
     3421 cccaaatcgt cggttagttg gggcgttccg gtcccaggca atccagatca cacgatgtac
     3481 gtttggttcg aggcgctatc caattacatc acagcaattg ggttcggtaa cgaggagaga
     3541 gagaaggcgg ccggttttga aaagtactgg cccgcactgc atctggtggg gaaagacatt
     3601 cttcgctttc acgccgtgta ttggcccgcg ttcttgttag cggctggcgt ggaattgccg
     3661 cgagggattg tcgcgcatgg catgtggctc gatccgaatg ggcgaaagat gtccaaaaca
     3721 atcggaaaca caattgagtt gaacctttta cagcagcact ttccagtcga tgccgttcgt
     3781 tatttctgcc tgcgcgaaat ggtgtttggg caggatgggc gtttcgggta cgagagcctt
     3841 atcgatagga ccaatagcga tctggcgagt gggctcggta atctatcgag tcgaactctc
     3901 acgatgattg cccgctactg cgatggtcac atcccttccg gacagatctc tgaagacaaa
     3961 cttttgctcg ctaaaagaag cggggttgat acagacgaga caaccgtagc aggcttcatc
     4021 gaaactgcgc gcgatcagta cctccagcat ttcgagagct ttgctttcag taaggcgtta
     4081 gaaacagcct ggtcggttgt gtctcgagtc gacaaaatca ttaccgacgc tcgaccctgg
     4141 gatcttgcaa aagacgaaaa tcaaaaacag acgctcaacg cagtgctcta tcgcgccgct
     4201 gaaacgctgc gctggctctc ggtcttgctc tatccggtaa tgcccgcagc ggctaatgaa
     4261 gtgtggagtc agcttggctt gagtggctcg ccgtcaaaac tcgacccgtc cagtttgaaa
     4321 tggggcgaat tgccggaagg ctccgtgtta ggggaagtga aaccaatttt tcctcgcatt
     4381 gataaggcta aaacaatggc ggagattact gaacaaacta agcctcgtgg cgagcacggt
     4441 acccaggcag acgcggttga cgtcgcggag gtctcgggcg ccacagctcc agtagctccc
     4501 ccgaaagatg caaccgaagc agaggcggtt ggagtcacga gcttcattga gatcgatgat
     4561 ttttcgaagg tcgacttgcg ggtgggccag gttttgtcgg cggaacgcgt tcctaaagcc
     4621 gacaaacttc ttttgctcaa ggtggatgtt aacgaggagc agccgcggca aattctggct
     4681 ggcatagctc aatactatga acccgaaaaa ttgataggtc gtaaggttgt cattgtcgcc
     4741 aatctgaagc caaggaagtt gcgagggttc gagtctcagg gaatggtggt tgccgcatca
     4801 tttggaccag agggacgtcc tgtaatagca acttttaccg aggacgtgcc taacggcgcg
     4861 cgtctgaaat gacttcctgt tatgttcatt gactcgcatg cgcacgtcga tggtcctgag
     4921 tttgatcaag atcgactgca agttctcgag cgtgcccgac tggcaggtgt taccacaatt
     4981 ctcaatgtcg gcactggaga tcctcacagt gatgctttcg acagagcagt cgagttaggt
     5041 gaaagtgaag aggccatcta cacagcgata ggaactcatc ctcatgatgc tcgactttac
     5101 ga
//
LOCUS       DASSFP010000232         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_799627, whole genome shotgun sequence.
ACCESSION   DASSFP010000232 DASSFP010000000
VERSION     DASSFP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799627"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFH91_05090"
     CDS             <1..515
                     /locus_tag="VFH91_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6862395.1"
                     /translation="PIALAMMNAGGLRKNEIKAGEIRASDIFELLPFENTLIAVDLTG
                     AQLAKLIPLGVRDAQSGARIHFKWNEQNKAEFISAKLLDSTGRETDIEPDKVYTIVTI
                     DYLYKLNSGSYAILQEAKKVTPLDVTVRDAMMDYVKAESAAGRSFQAKGKDRFVQIGP
                     DPTGPVVPYD"
     gene            508..1431
                     /locus_tag="VFH91_05095"
     CDS             508..1431
                     /locus_tag="VFH91_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HET6862396.1"
                     /translation="MINRRKFLTNSAVFGAVLVATPRRFASEAWVADTIITVLHTNDT
                     HSQIEPLPANDKNAGRGGVARRATLVKRVRQQNPNTLLVDAGDVLQGTPYFNFYKGEV
                     EYKAMSAIGYDVGTLGNHEFDNGVEALATALKFANFDIVSANYNVKGTPLENRVKRYV
                     VKTVAGIKVGIFGLGISPVALITPASFQGITYEDPIVTAKETVKILRENERCSLVIAL
                     SHLGYYPNPKQGSIGDSQLAAQIDGIDFIASGHTHTFMQEPVVQVQPCGEKTLIFQVG
                     KSGIYVGRVDFTFRAGKLVASNGSLMDLREA"
     gene            1445..2212
                     /gene="rpiB"
                     /locus_tag="VFH91_05100"
     CDS             1445..2212
                     /gene="rpiB"
                     /locus_tag="VFH91_05100"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET6862397.1"
                     /translation="MADDTTRDRVRALVKEVLAKTPVEPEADVSMSRFIDTVPQSSAD
                     ARVTKDESAKNVITEDDVRDLSPGSVLRIAENARLTSLAADLVKQNKIELVRRTPRRG
                     SNAIKLVAIGSDHGGFKMKEELASLLRDLGHTVHDFGTNSEEPVDYPDFAHAVARAVA
                     DRTSDVGIIIDGAGVGSAMTANKVPGVRAAACYSVAVAQNSREHNDANVLTLGSKTIT
                     SGEMREIVTAWLQTSISEDRHRKRVAKIEAVERQYQR"
BASE COUNT          580 a          522 c          603 g          538 t
ORIGIN      
        1 aaccgattgc actcgccatg atgaatgcag ggggcctgag gaagaatgaa atcaaagcag
       61 gtgaaattcg agcgagcgac atcttcgagt tactgccatt cgaaaacacc ttgatcgcag
      121 tagatctgac cggagctcaa ctggcgaaac taataccact tggtgttcgc gatgcccaat
      181 ccggagcgcg tattcatttc aaatggaacg agcagaacaa ggcagagttc atcagcgcga
      241 agttactcga ttctacggga agggaaacgg atatcgagcc cgacaaggtt tataccatcg
      301 taactatcga ctacctttac aaactcaaca gcggttccta tgcgattctt caggaggcaa
      361 aaaaagttac gcctttggac gtaaccgttc gtgatgccat gatggactac gtcaaagctg
      421 aatctgctgc gggtcgatct ttccaggcta aaggcaaaga tcgttttgtt cagattggtc
      481 ccgatccaac tggtcctgtg gttccttatg attaaccgac gaaaatttct tactaactca
      541 gctgtatttg gtgcggtgtt ggtcgcgacg cctcgtaggt tcgcctctga ggcatgggtt
      601 gctgatacca taatcaccgt ccttcacacc aatgacaccc actcgcagat tgagcctttg
      661 ccggcaaacg ataagaacgc tggaagaggt ggagttgcgc ggcgagcgac cttagttaag
      721 cgagtcaggc aacaaaatcc aaatactttg ctggttgacg ccggagacgt tttgcaaggc
      781 actccttatt tcaacttcta caaaggtgaa gtggagtaca aggcgatgtc ggcgatcgga
      841 tatgacgttg gcacattggg aaaccatgaa ttcgacaacg gtgtcgaagc ccttgccact
      901 gctctgaagt tcgcaaattt tgatatcgtc tccgccaatt acaacgtcaa gggaacgccg
      961 ctcgagaatc gggtgaaacg ttacgtggtg aagaccgtgg ctggaataaa ggtgggcatt
     1021 tttggtttgg gtatcagtcc ggtggcattg attacccccg catccttcca gggtattacc
     1081 tatgaagatc cgatcgtcac cgcaaaagag acagtgaaga tcttgcgcga gaacgagcgt
     1141 tgctctctgg tgatcgctct ttcgcatctc ggctactatc ccaatccgaa acagggatca
     1201 atcggtgatt cgcaactggc ggctcaaatt gatggtattg atttcatcgc cagtggtcac
     1261 actcacacgt ttatgcagga gcctgtggtc caggtccaac cctgcggtga aaagacatta
     1321 atatttcagg ttggaaagag tgggatttat gtcgggcgcg tcgattttac tttccgcgcc
     1381 ggcaagttgg tcgcgtcgaa tggtagtttg atggatttgc gcgaagctta ggtgagctca
     1441 atctatggct gatgatacaa ctcgcgaccg tgtccgcgcc ctggtgaagg aggttctcgc
     1501 taaaactcct gttgaacctg aagcggacgt ttcaatgagc aggtttattg ataccgttcc
     1561 acaatcgtct gctgatgctc gagtcacaaa ggatgaatcc gctaaaaatg tcatcactga
     1621 agatgacgtg cgggatttgt cgccaggttc ggtcctccgc attgctgaaa acgcgcgtct
     1681 gacttcgctc gccgctgatt tagttaagca aaacaaaata gaactggttc gacgcactcc
     1741 acgccgtggt tcaaatgcca tcaaattggt ggccattgga tctgaccacg gtggcttcaa
     1801 aatgaaagaa gagctggctt cgttactaag agacctgggc cacaccgttc atgattttgg
     1861 cacgaattcc gaagagccgg tcgattatcc ggattttgct cacgctgtag cacgagcggt
     1921 tgccgacaga acttcggatg ttggaatcat catcgatggg gccggggttg gcagcgctat
     1981 gaccgctaac aaggtacctg gggttcgtgc ggcggcgtgt tactcagttg cggtggcaca
     2041 aaattccagg gaacacaatg atgcgaacgt cctcacgttg ggaagtaaaa ctattaccag
     2101 cggggaaatg cgggagatag taactgcttg gctgcagact tcaatcagcg aggatcggca
     2161 ccgcaagaga gttgcgaaga tcgaagcagt ggaaagacag tatcaacggt aagcggaagt
     2221 tctaagttac caatcaaaga gaa
//
LOCUS       DASSFP010000233         6429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2146294, whole genome shotgun sequence.
ACCESSION   DASSFP010000233 DASSFP010000000
VERSION     DASSFP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146294"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFH91_05105"
     CDS             <1..368
                     /locus_tag="VFH91_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862398.1"
                     /translation="KLETVALNGEEIVVKSGEELSDLTIVLSYASASLRGTIKVPASD
                     LVGKLSGQVFLYSDKTLIAYGPIDSRGEFIFQDIPAGNFKLVTTVDVPGSKTLKAEQF
                     VDVVAGTMSEVTVTPLNQN"
     gene            568..1155
                     /locus_tag="VFH91_05110"
     CDS             568..1155
                     /locus_tag="VFH91_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862399.1"
                     /translation="MRRFVPQLFAQIFCISLLLLLAPNSNAQLPIRPSIGRPDIQSRE
                     WELGHLRPEVKAQYDRQQQARVARREDFRKLQIVNNALMQRMFLASKNNTPKLTPKEI
                     SSSLEEIKKVAQRLRTNLPIPRLETDKDLTSEAVTLSPGLLRLDKAVMSFVENPLFSQ
                     PRVYDSEMATKAGKDLNEIVRLSEFLRKLSKVKHD"
     gene            complement(1275..2717)
                     /locus_tag="VFH91_05115"
     CDS             complement(1275..2717)
                     /locus_tag="VFH91_05115"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET6862400.1"
                     /translation="MSQLQNSIRVMKFGGTSVQDADCIRRAAEIIASNRGPVVTIVSA
                     MGGVTNRLIEAAHKSATGDETAGQNVKDAVLQQHLNAIGELVDDDDTRRELANDISGV
                     VSRVADLCRGTSLLRELTPRALDDISSAGERLSARLVAAAVRQLGVDSVALEATNFIV
                     TDAQHGQAEPLMEKTREAVRANLLPVLAAGVSPIVTGFIGATEEGRLTTLGRGGSDYS
                     ATILGAALDAGEVIIWTDVDGVLSADPRLVTQARILNEISYNEAAELAYFGAKVLHPK
                     TLRPVTEAGIPVWIRNSFAPENRGTKITSNGNPSATGVKAVTAFSDVALITLGGRGIV
                     GVPDVLAKVFAATASVRANVLLISQASSQNDICFVLHAADVEPTVAALREKLALELMQ
                     QKVEHISVDKHVAIVAVVGERMRGSLGVSGRTFSSLAAAGVNVIAIAQGSSEYNISFV
                     IEATATKNAVDAVHQEFRLHERSVVHTEAI"
     gene            2979..3521
                     /locus_tag="VFH91_05120"
     CDS             2979..3521
                     /locus_tag="VFH91_05120"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET6862401.1"
                     /translation="MGFKVGQKVVYPNHGIGTIEQIEQKQIGATLLPFYTLRLAANNS
                     LVLVPVSNASEVGLRAPISSGECDMLLKALADDFTSPANDWKDRFKEFSEKMRTGDIF
                     EVADVLKHLTYLSNLKPLSFREQRMLERSRYLVVSELAAVCRQPECNIEPRVDQAIAR
                     ACTKHDRRPLRRAAGAASGH"
     gene            3685..5070
                     /locus_tag="VFH91_05125"
     CDS             3685..5070
                     /locus_tag="VFH91_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6862402.1"
                     /translation="MLCLIAAQADVTSHNTGSILLRLIAVLFLVAANGFFVAAEFALV
                     GVRSSRIETLAATGSRSAKRLLLQLQELNQYLSACQLGVTLASLALGWIGEPAIARIL
                     EEPLSGLSDTFRHGIAFAIAFSIITTLHIVLGEQAPKLMGLARAEKVALAIALPLQVF
                     YRLFSLPIRALDWASARTVRLVGIEATEDQASTYTEAELRRLIDISRESGHLRAEERR
                     LIHRVFEFSDTVVREAMVPRTEMAAISSTCSLEQITKVFEQNRFSRLPVYRDSLDDVI
                     GFIHSKDVMPYLLHPENFKLEDVLQPPMYVVDTARLEHVLRQMQKAKMHFGFVVDEHG
                     GLEGIITLEDLLEEIVGDISDEHDEEVNEQITEITKDNYVLAGNLAVRDLNRRLKLSV
                     PESEAYTTIAGFLMTQAGHVLRTGEVVEYDGLLFKVERVERRRIMRVKLEVLDKVHDT
                     EPIASSVTPRA"
     gene            5183..>6429
                     /gene="eno"
                     /locus_tag="VFH91_05130"
     CDS             5183..>6429
                     /gene="eno"
                     /locus_tag="VFH91_05130"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET6862403.1"
                     /translation="MSWIEQVVAREILDSRGNPTVEAEVVLEDGEIGRAAVPSGASTG
                     EHEAVELRDGDKKRYGGKGVLKAVRNINDLIAPEIEGMDALDQAEIDATLIELDGTKT
                     KSKLGANALLAVSLATARAAAIHQELPVYRYLGGPNARTLPVPMMNIINGGAHADNNV
                     DFQEFMVVPVGAARFSEALRMGTEVFHELKAVLKKKGYATSVGDEGGFAPNLRSNEEA
                     IETILESISKAGYKPGEDCLLALDPAASEFFDGKAYVFKKSDKRKLNSSEMVAFWKSW
                     CDQYPIVSIEDGMAENDWTGWKQLTDEIGESVQLVGDDLFVTNTSFLEKGIELGVANS
                     ILIKVNQIGTLTETLDCIELAQKNRRTAIISHRSGETEDPFIADLAVATNAGQIKTGS
                     LSRTDRIAKYNQLLRIEEDLADAA"
BASE COUNT         1619 a         1636 c         1624 g         1550 t
ORIGIN      
        1 taaaactaga aacggttgcg ctgaacggcg aagagattgt ggttaagtct ggagaggagc
       61 tttccgattt gacgatcgtt ttgtcgtatg cgagtgcgag ccttcgagga acaatcaaag
      121 ttccagcgag tgatcttgtc ggaaaattga gcggacaggt ttttctttat tcagataaga
      181 ctttgatcgc atacggaccg atcgattcac gaggagaatt tatttttcaa gacattcccg
      241 caggtaactt caaactagtc acgacggttg acgtgccggg ctcgaagaca ttgaaagcgg
      301 agcaattcgt tgacgtcgtc gctggaacga tgtccgaagt tacggtaact ccactcaacc
      361 aaaattagag acccgatatt atttagaaaa tcacgaatat cgaatagctc accctccaac
      421 tccatctaat ttattggatt gttatgcaaa tttctgttgc ctgattttcc cttcaaatat
      481 atactgcgcc cgcttcaagc cggttgatcc cttcgttttg tccttcgaaa acttgttaac
      541 acctgattgc ctaagaggag gtgtcacgtg cgacgatttg ttccccagct cttcgcgcaa
      601 atattttgta tctctctact ccttctgctt gcacccaatt caaatgccca actacctatt
      661 aggccaagta tcggccgccc ggatattcaa agtcgagagt gggaactcgg gcacctgcgg
      721 cctgaagtca aagcccagta cgatcgccaa caacaagcca gagtcgctcg tcgggaagac
      781 tttcgtaaac tgcagatagt gaacaatgcc ttgatgcagc gaatgtttct ggcctctaag
      841 aacaatacgc ccaagcttac tcctaaggag atctcctcga gtttagagga gatcaaaaaa
      901 gtagctcaac gattgaggac taatctcccg attccgcgct tagagacgga taaggatctc
      961 acttcagagg ctgtcacctt atcaccagga ctcctgaggc tcgataaagc tgttatgagc
     1021 ttcgtggaaa atcctctgtt ttcgcagcca cgagtctacg attcagaaat ggcaaccaaa
     1081 gcaggtaaag atctcaatga gattgtgcgg ttgagtgagt ttctccgaaa actttcaaag
     1141 gttaagcacg actaatccac ggattatgca aattacccgg attttcagct gaacggctgg
     1201 acataactcg tccgcgtttt gaaaaatcgg tgtaatcagg cgcaatctgt gaatgattct
     1261 ctttgctctc ttgattagat cgcttcagtg tggacgacgg atctttcgtg tagcctgaac
     1321 tcctgatgca ctgcgtcgac tgcgtttttt gttgcagttg cctctatgac aaaggagata
     1381 ttgtattcgg aagagccctg agcaattgcg attacattca ctccagcagc agccaacgaa
     1441 ctaaaggtgc gacctgaaac tccgaggctg ccgcgcattc tctcacccac tacagccacg
     1501 atagcgacat gtttgtcgac actgatgtgt tcgacttttt gttgcatcag ctcaagtgca
     1561 agtttctctc gcagagcagc aacggttggt tcaacatctg cagcatgaag aacaaaacag
     1621 atgtcgtttt gtgacgatgc ctgcgagatt aagagtacat ttgcccgcac gctggcagtc
     1681 gcggcaaaga ctttggctaa cacatcagga acgccgacga tgccacgccc gccaagcgta
     1741 attaaggcta catcactaaa tgctgtgacg gccttgactc cggtcgctga cgggttgccg
     1801 ttggaagtga tttttgtacc gcgattctcg ggtgcaaagc tgtttcgaat ccacaccgga
     1861 ataccggctt ccgtcactgg ccgcagagtc ttaggatgaa gcaccttggc gccgaaatag
     1921 gccaactctg ccgcttcgtt gtaagagatc tcgttaagga ttcgcgcctg agtaacgagg
     1981 cgcggatcag cggaaaggac gccatcaaca tcagtccaaa taatgacctc gccggcatcg
     2041 agtgcagcgc ccaggatcgt tgcagaataa tctgagccgc cacgacccag agttgtaagt
     2101 ctgccttctt cagtcgcacc tataaacccg gtaacaatgg gtgaaactcc ggccgcgagc
     2161 acgggtaata ggtttgcccg gaccgcctca cgagtttttt ccatcaatgg ttcggcctga
     2221 ccgtgttgtg catcggtaac aataaagttc gtagcttcaa gcgcaacact gtcaacgccc
     2281 agctgccgta cggcggcggc gacaaggcgc gctgagagtc gttcaccggc actggaaata
     2341 tcgtcgaggg cacgaggagt caattcccgc agcaaagacg tgccccgaca cagatcggct
     2401 acgcgactaa cgactccgga gatgtcattg gcgagttctc gacgggtgtc gtcatcatcg
     2461 actaattcac caatggcatt caggtgctgt tgtagcaccg cgtccttgac gttctgtcca
     2521 gctgtttcat cacccgtggc tgatttatgg gccgcctcga tcagacgatt ggttactccg
     2581 cccattgctg aaacaatggt cacgaccggg ccacggttcg aggcgatgat ttcagcggca
     2641 cgacgaatac agtcagcgtc ctgcactgac gtaccaccaa acttcataac gcgaatgctg
     2701 ttttgtagtt gggacatttt ctcgaatcag gagtttactg gagtgcaggt tacaatagcg
     2761 aagccggaaa cgaatttatg cgagcataaa ctgatgcgac ctggacgctt ttgctcaatg
     2821 gaagcctgaa ccccctcaaa accttgacgc tgcgcaagac cgcttgctag acttcaatta
     2881 ctttccaaac agggcacgac gctatctgcg tcgctcagat tctcctagat tcgcgcgtgc
     2941 ggcgcagtct gatttttcac aatcgatgga ggtgcatggt gggctttaaa gtcggccaaa
     3001 aagtcgttta cccgaaccat gggataggga ccatcgagca gatagaacag aagcaaatag
     3061 gtgcaacatt actaccgttc tacacgcttc gtcttgctgc caataactcc ctggtcttag
     3121 ttcccgtctc aaatgcctca gaagttggcc tgcgcgcccc aatctcgtcg ggcgagtgtg
     3181 acatgctgtt gaaggcctta gcagatgatt tcacttcccc cgctaacgat tggaaggacc
     3241 gcttcaagga attttcggag aaaatgcgca ccggcgatat tttcgaggtc gctgatgtgc
     3301 tcaaacacct gacctatcta agcaatctca aacccctctc atttcgtgaa cagcggatgc
     3361 tggaacgctc tcgatacctt gttgtttccg aattggctgc agtttgccgc caacccgagt
     3421 gcaacattga gccccgagtt gaccaggcta ttgcccgagc ttgcacaaaa cacgataggc
     3481 gtccccttcg tcgtgcggcc ggcgctgctt caggacatta aagaattttc cgatcgtaac
     3541 tggcttcctg gcttcggttt cgcagcgtac tgatctgcgc accccaggcg gcgcgtgtta
     3601 tcattcgcca gtcttgaaac gaagggatga agcggctggc gatccttact cgcctttctc
     3661 agaatggacg gattaccaag tagcttgttg tgcttgatcg cggctcaggc tgacgtgact
     3721 tcgcacaaca ccggctcaat attactaaga ttaattgccg tccttttttt ggtcgccgct
     3781 aacgggtttt ttgtcgctgc cgaattcgcc ctggtaggag ttcgctcttc tcgtattgaa
     3841 accctggccg cgacgggcag ccgctccgca aaaagactgc tgctgcaact ccaggaactc
     3901 aaccaatacc tctccgcttg tcagttggga gttacactcg cttcgctagc cttaggatgg
     3961 atcggtgaac ccgcgatcgc tcgtatactc gaagagcctc tatcgggtct atcagatacc
     4021 tttcgccatg gaatcgcctt cgctatcgcg ttttcgatca tcaccacatt gcacattgtc
     4081 ctcggtgaac aagcgcctaa gttgatgggt ttggctcgcg cggaaaaagt tgcactcgcg
     4141 attgcgcttc ctcttcaagt tttttaccga ttgtttagct tacccattcg cgcgttggat
     4201 tgggcgagcg caaggaccgt acgcctggtg ggaatcgaag caacagaaga ccaagcttcg
     4261 acttataccg aagccgagtt acggcgattg atcgacattt cgcgtgagag tggacacctg
     4321 cgagctgaag aacggcgact cattcaccgg gtcttcgaat tttctgacac agtggtccgc
     4381 gaagcaatgg ttccgcgaac agagatggcc gccatctcaa gcacctgctc actagaacag
     4441 atcactaaag tatttgaaca gaataggttt tctcggcttc ctgtttaccg cgactcgctc
     4501 gatgacgtaa ttgggttcat ccacagcaaa gacgtcatgc cctatctgct ccacccggaa
     4561 aatttcaaac tggaagacgt tcttcaacca ccaatgtacg tggtggatac agcaaggctg
     4621 gaacatgtgt tgcgacaaat gcagaaggca aaaatgcatt ttggattcgt ggtggacgaa
     4681 catggcggac tggaaggaat catcactctc gaagatctgc ttgaagagat cgttggcgac
     4741 atttctgacg agcatgatga agaggtaaac gagcagatca cagagatcac gaaagacaac
     4801 tacgtcctcg ctggtaatct tgccgttcgc gacctcaatc gacgtctaaa actttcagta
     4861 ccagagtcag aggcttatac aacgattgcc gggtttctca tgacccaggc aggtcacgtc
     4921 ttaagaaccg gcgaggtggt tgagtacgat ggcttgctct tcaaggtcga gcgcgtggaa
     4981 cgacggcgga taatgcgcgt gaagctcgaa gtgttggaca aggtccacga caccgagcca
     5041 atagccagct ccgtcacgcc gcgagcctga tgcaggaaac agctcggttt gcatcctggg
     5101 cctggaaatg aactttcccg ttagtacgtg ttaaagtctc accgcaaact taaccaaact
     5161 ttatttcaca ggagcagaac gcatgagctg gatcgagcaa gttgtcgctc gcgaaatttt
     5221 ggattcccga ggcaatccca ctgtcgaagc cgaagttgtt ttggaggacg gcgagatcgg
     5281 ccgtgcagca gttccatccg gggcgtcgac cggcgagcat gaagccgttg agttacgtga
     5341 tggtgataag aaacgctatg gcggcaaggg cgtcttgaaa gctgtgcgaa acatcaacga
     5401 cctcattgcg cctgagatcg agggaatgga cgcgttggac caagccgaaa ttgatgcgac
     5461 cttgatcgaa ctagatggaa cgaaaacaaa atcgaagctc ggggccaatg ctttactcgc
     5521 agtttcgtta gcaacagcac gagccgcagc tatccatcaa gaactgccag tgtatcgata
     5581 tcttggtgga ccgaacgccc gaactttgcc cgttccaatg atgaacatta tcaacggtgg
     5641 tgctcacgct gataataacg tcgacttcca ggaatttatg gtcgttccgg tgggagcggc
     5701 ccgattcagc gaggctctac gaatgggaac cgaagtcttt catgagttga aagcagtcct
     5761 caaaaagaag ggttacgcaa caagtgtcgg tgatgaaggc ggtttcgctc caaacctgcg
     5821 gtccaacgaa gaagcgattg aaacaattct cgaatcaatc tcgaaagcgg gatataagcc
     5881 tggcgaagat tgtttgctcg ccctggatcc agcagcaagt gagttcttcg acggcaaagc
     5941 ctacgtcttt aagaaaagcg ataagcgaaa actcaacagt tcggaaatgg tcgcattttg
     6001 gaaatcatgg tgtgaccagt atccaatcgt ctcgattgaa gatggcatgg cagaaaacga
     6061 ctggaccggt tggaaacaat taactgatga aattggtgag agcgtgcaac ttgtcggtga
     6121 cgacctgttt gtaacgaata cctcgttttt ggaaaagggg attgagttgg gagtcgccaa
     6181 ctccatcctg attaaagtca atcagattgg cacacttact gaaactctcg actgcatcga
     6241 gctagcgcaa aagaatagac gcacggccat catctctcat cgctctggcg aaactgagga
     6301 tccgtttatt gcggatctcg ccgtcgccac gaatgccggt cagataaaga ctggctcact
     6361 ctcgcgtacc gaccgcattg ctaaatacaa tcaactatta cgcattgaag aggaccttgc
     6421 tgatgctgc
//
LOCUS       DASSFP010000234         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_212980, whole genome shotgun sequence.
ACCESSION   DASSFP010000234 DASSFP010000000
VERSION     DASSFP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212980"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2592
                     /locus_tag="VFH91_05135"
     CDS             <1..2592
                     /locus_tag="VFH91_05135"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HET6862404.1"
                     /translation="EQTLKEARVIELINAYRVRGHLIADIDPLHALPVLYHPELDIET
                     YGLTIWDLDREFITDGLANRESDTLREILDILQRAYCGKVGVEYRHIQSKEEKTWIRE
                     QIRREFVEPKPISVEIKKKLLWKLISAEQFERFLHTKYLGQKRFSLEGCETVIPVLDR
                     LIEGAAEQGVEDITLGMAHRGRLNVLANVVGNLCERIFAAFEGSVHPEFPADEGDVKY
                     HQGATGERVTETGRKVSLTVSPNPSHLEAVDPVVEGMVRAKQDERLGKEQLSRDEVMD
                     LAMPILLHGDAAFAGQGVVMETLNLAQLKGYRTGGTIHIIINNQIGFTTSPEAGRSTI
                     YSTDVARMTQLPIFHINGDDPEAAFRVLTIALDYRQRFNKDVVLDLIGFRRLGHNETD
                     EPSYTQPLMYARVKAHPGVRTIYAKRLVKEGVITEEEVDQLIQERVKRYESALANAKR
                     KVTEEPAQAQPETKEFEGSIIVPTAVSTETIKTIAHKIAVVPEGFNVNPKMVGQLARR
                     AKMGEGSLPIDWAFAEAIAFGSLVLDGTQVRLSGQDSGRGTFSQRHAVLYDTQTGKSW
                     CPLAELRNDSHPDARFEVFDSSLSEEGVLGFEYGYSVVARDALVMWEAQFGDFGNGAQ
                     TIIDQYIAASEDKWKQRSRLVMLLPHGYEGQGPEHSSARLERYLQLCAENNLAVCYPT
                     TPAQYFHLLRRQTQPGMERPLIVMTPKSLLRLPAAASTLNDLSTGGFEPVIDDKEVSD
                     ASSVQKIVFCSGKVYYDLIEARKKSGEHGVAIIRLEQFYPFPLQRIQEILKRYTSAKR
                     LAWAQEEPQNMGGWTFIQERLENLLPACERPQYVGRSASASPATGAYHIHQKEQATLI
                     SEALSLP"
BASE COUNT          735 a          695 c          755 g          608 t
ORIGIN      
        1 gagcaaactt taaaagaagc cagagtcatt gagctgatca acgcatatcg tgttcgtgga
       61 cacctgatcg cagacattga tccactccac gcgctaccag tgctctatca cccggagctg
      121 gacatcgaaa cttatggtct aacaatctgg gatctcgatc gcgagttcat tactgacgga
      181 ttggcgaaca gagaaagcga cacgctgcga gagatactcg atatacttca gcgagcttac
      241 tgcggaaaag tcggggtcga atatcgtcac attcaaagta aagaagagaa aacctggata
      301 cgcgaacaga tcagacgtga gtttgtcgag cccaaaccca tatccgttga aatcaagaag
      361 aagctacttt ggaagttgat ctccgcagaa cagtttgaac gatttcttca cacgaagtat
      421 ctgggacaga agcggttttc actcgaaggc tgcgaaacag taatcccggt actggatcgc
      481 ttaatcgaag gggcggcaga acaaggtgtt gaggacatca cgctcggaat ggcccatcgg
      541 gggcgtttga atgtactggc caacgttgta ggaaatcttt gcgagagaat ttttgccgct
      601 tttgaggggt cggtacatcc tgaatttccc gccgacgaag gtgatgtcaa ataccaccag
      661 ggcgcgacgg gcgagcgggt caccgaaaca ggtcgaaaag ttagtctcac cgtttctccg
      721 aatccgagcc atctggaagc tgtggatccg gttgtcgaag gtatggttcg agctaagcag
      781 gatgagcgtc ttggtaagga gcaattgagt cgcgacgaag tcatggacct cgcaatgccg
      841 atcttactgc acggcgacgc agcttttgcc gggcaaggcg ttgtcatgga gacgctgaac
      901 cttgcgcagt tgaagggtta tagaaccggc ggaacaattc acatcattat taataatcaa
      961 atcgggttca caacttctcc cgaagccggt cgctcaacta tctattcgac ggatgtcgcg
     1021 cggatgacgc agttaccaat cttccacatc aatggtgacg atcccgaagc agcctttaga
     1081 gtattgacga tcgctcttga ttaccggcag cgattcaaca aagacgtggt actcgacctt
     1141 attggattcc ggaggctggg ccacaacgag actgatgaac ccagttacac gcagccgttg
     1201 atgtacgcca gagtaaaagc tcatccgggc gtccggacca tttacgcgaa gcgactagta
     1261 aaggaaggcg taataacgga agaagaggtc gaccaactaa tccaggagcg cgtcaagcgt
     1321 tacgaaagcg cgctcgcgaa cgctaaacgg aaagtcaccg aggaaccagc gcaagcccaa
     1381 cctgaaacta aggaatttga aggctccata atcgttccaa ccgccgtttc tacggaaaca
     1441 attaaaacta tcgctcataa gatcgccgtg gtcccagaag gttttaatgt caatcccaag
     1501 atggtgggcc aattggcacg acgggccaag atgggtgaag gcagcttgcc gatcgattgg
     1561 gcttttgccg aggcaatcgc tttcggttct ctcgttctcg acggcacaca ggtccgcttg
     1621 agtggtcagg actccggccg gggcactttc agccaacgac atgccgtgct ttatgacact
     1681 caaaccggaa aatcatggtg cccattagcg gagctcagaa acgacagtca tcccgatgcg
     1741 cgatttgagg tattcgacag ttcgttgtca gaggaaggcg tgctcggttt tgagtatgga
     1801 tattcagttg tcgccaggga tgcgttggta atgtgggaag cacagtttgg tgattttgga
     1861 aatggcgcgc aaacaatcat cgaccaatac atcgcagctt ctgaagacaa atggaaacag
     1921 cgttcacgtc tggtgatgct gctgccacac ggctatgaag gtcaggggcc agaacattcc
     1981 agtgcgaggt tagagcggta cctgcaactt tgcgcggaaa acaatcttgc cgtttgttat
     2041 ccgacgactc cggcgcagta ctttcatttg ctacgacgac agacccagcc tggcatggag
     2101 cgcccgttaa ttgtcatgac tccgaagagc ctgttgcggt taccggccgc tgcttcaacg
     2161 ttaaacgacc tgagcaccgg aggatttgag cccgtcattg acgataagga agtatcggat
     2221 gcaagttcgg tccagaagat cgtcttctgt agcggcaagg tctattacga tctgatcgaa
     2281 gcgagaaaga agagtggcga gcatggcgtg gcgattattc gactcgaaca gttttatcct
     2341 ttcccgcttc aaagaattca ggagatcctc aaacggtaca cctcagcaaa acgactggcc
     2401 tgggcacaag aagagccaca aaacatggga ggctggacct ttattcagga gagactggaa
     2461 aatctgctac cggcttgtga gcggccacaa tacgtcggcc gttcggcttc agctagcccg
     2521 gctaccggcg cttaccacat tcatcagaag gaacaggcca cactgatctc cgaagccctg
     2581 agccttccat agaataatac ctttacaccg caaagtactt gaccccgcta tcggaatcgg
     2641 tttaggatgc gagtcatgga tcctttgcga gccatttccc ccgccccgga tcagccgccg
     2701 gccttgcatg agcgggcgat ggacaatctt cgctacatcc gtgaaacgat ggagcgagca
     2761 actgccttta ccggaatatc cggatggggt gaa
//
LOCUS       DASSFP010000235         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_213537, whole genome shotgun sequence.
ACCESSION   DASSFP010000235 DASSFP010000000
VERSION     DASSFP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213537"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFH91_05140"
     CDS             <1..197
                     /locus_tag="VFH91_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862405.1"
                     /translation="GILETRRGVGTFVCAHQFQANNRQDHENQLRELVDRFVEEASAM
                     GFTPDDLLDQLQTRRKKETK"
     gene            199..1131
                     /locus_tag="VFH91_05145"
     CDS             199..1131
                     /locus_tag="VFH91_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET6862406.1"
                     /translation="MSSTNSLIQTRNNLPATNAPRVNVVSAVAFLVPAILGVLLTVSY
                     LSPFGAIAGVLLGILFAQAPKIARQWERAVILRLGRYVGLRGPGLFFVIPFVDTVSAW
                     IDQRVITTSFAAEETLTSDTVPVNVDAVLFWMVYDPEKAALEVQNYPQAVSWAAQTAL
                     RDIIGRTSLTDLLRGRERIEDELQQLIDERSNPWGVTVQSVEMRDVIIPAALQDAMSR
                     EAQAAREKQARIILGQAEVEIANSFEIAARSYRDNPTALHLRAMNMLYEGLKEKGALM
                     LVPSSAVESMGMGGLLGAAALRQQTLTGSDEVPE"
     gene            complement(1209..1478)
                     /locus_tag="VFH91_05150"
     CDS             complement(1209..1478)
                     /locus_tag="VFH91_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862407.1"
                     /translation="MEIDDDAKEIVREIGTAINESVENSTKVAEAIERLREAGYEMEL
                     TLRLEIGLRPHAGDDEEEQSKATLDLTDEDIQVLQRMKIRIDPEL"
     gene            1687..2793
                     /locus_tag="VFH91_05155"
     CDS             1687..2793
                     /locus_tag="VFH91_05155"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET6862408.1"
                     /translation="MLGTIGILTGGGDAPGLNAVIRAVVKTATCEHGMRVIGIEDGFE
                     GLLGATQTRLLLPGDVRGLLPRGGTILGTRNRGNFVERTKDGVPMSVEAVYQEAISNM
                     RVLGIDALVVLGGEGTLGIAAEFDQRGIPVCGVPKTIDNDLACTELTFGFVTALDIAT
                     EALDRLHTTAESHDRVMILEVMGRHAGWIALHSGIAGGADVILIPEIPFSREAVAEKI
                     RVRENAGKNFSIIVVAEGAVEIGHQLIYQDRGDARHAPRLGGIGVHLQRDLEALTERE
                     TRCVVLGHLQRGGRPNAFDRMLATNFGSCAVRALSQGEKGVMVALQAGDVVTVPLTEA
                     IKNIKSVPPNGQLVRTARDTGISFGSPDEANYHE"
     gene            2797..>3128
                     /locus_tag="VFH91_05160"
     CDS             2797..>3128
                     /locus_tag="VFH91_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862409.1"
                     /translation="MKKKERKSLSQQGIKDKLQGELDELESELRVHLPKEIKRALEFG
                     DLRENSEYRAALDRQNVVKARIVELRQRISEIASIDPNKVPRDKASYGSTLVLYDAEK
                     DEEVTYRLV"
BASE COUNT          781 a          756 c          849 g          742 t
ORIGIN      
        1 ccggaattct agaaacgcga agaggggtag gaacgtttgt ctgcgctcat cagttccaag
       61 caaacaaccg ccaggatcat gaaaatcaat tgcgggagtt agtcgaccgg ttcgtcgaag
      121 aagcttccgc aatgggtttc accccagacg atttgctcga tcaactgcaa acgaggcgca
      181 agaaggagac taagtaaaat gtctagtacg aacagcctga ttcaaaccag aaacaacctg
      241 ccagcaacta acgccccgcg agttaacgtc gtctccgcag tagcgttttt ggttccggca
      301 attctgggtg ttctattgac ggttagctat ttgagtccat tcggcgcaat cgccggcgtg
      361 ttgctcggaa tcctgtttgc acaggcgcca aagattgctc gccaatggga aagagccgtg
      421 atcctgcgcc tgggacgtta tgttggcctg cgtgggccag gattattctt tgtgatccct
      481 ttcgtcgaca ccgtctcggc ctggatcgat caacgtgtga tcacaacgag ctttgctgcc
      541 gaggaaactc tgacctcgga tacggtacct gttaacgtcg atgccgtgtt gttctggatg
      601 gtctatgatc cagagaaggc cgcattggaa gtacaaaatt atccgcaagc agttagttgg
      661 gccgctcaaa ctgctctgcg cgacatcatc ggaagaactt cgcttacgga tcttctgaga
      721 ggtcgcgaac gaattgaaga cgagttacag cagttgattg atgagcgttc aaacccatgg
      781 ggagtcacgg tacagtctgt cgagatgcga gatgtgatta tccctgccgc tctccaggac
      841 gctatgtcac gcgaggctca ggcagccaga gagaaacagg ctcgtatcat cctgggtcag
      901 gcagaagttg aaatcgcaaa ctctttcgag atagccgccc gttcttaccg ggacaatcct
      961 acggcgctac atctaagagc gatgaatatg ctgtatgagg gtcttaaaga aaaaggcgcg
     1021 ttaatgttgg ttccgagcag tgcagtggag tcgatgggca tgggaggact gctgggtgcg
     1081 gcggcgttac ggcaacaaac attgaccggt agtgacgaag tgcctgaata atcaaaatgt
     1141 ggtcgacgaa gtacaaaacc ctgcgactcg gactagtcgc agggtttttg atttttgctt
     1201 aacgtaaatt atagctcagg gtcgatcctg attttcatcc gctgcaaaac ctgaatgtct
     1261 tcgtctgtga gatcgagagt tgccttggac tgttcttctt catcgtcgcc ggcatgaggc
     1321 cgcaaaccga tctccagcct cagcgtcaat tccatttcgt agcctgcttc gcgcagccgc
     1381 tcgatggcct cggcaacttt ggttgaattc tctaccgatt cgttgatcgc cgtaccgatt
     1441 tctcggacta tctcttttgc gtcgtcgtct atttccaaat cgcgaatgcc ttcaatgtaa
     1501 aatgtttgaa gtagccgcta tgctacgcga ccgatttcag agggtcaacc caaggtctga
     1561 acttatttct atatctcact tttcaatttc acgttgaagc catcaagtta tagactgtct
     1621 tgaaactctt tggaccggtg gacattctcg ggccaacgca gcggatgagt gagatagagc
     1681 actgaaatgc taggaaccat cggaatacta acgggcggcg gcgacgcgcc tggtcttaac
     1741 gccgttatta gagccgttgt taagacggcg acttgcgaac acggtatgcg agtgattggt
     1801 atcgaggatg gttttgaagg actcctcggc gcaactcaga ctcgactctt attgcccggt
     1861 gacgtacgcg gattactccc gaggggaggg actatactgg gcactcgcaa ccgcggaaac
     1921 tttgtcgaga gaactaaaga tggcgttccg atgagtgtcg aggccgtgta tcaggaagcc
     1981 atctctaaca tgagagtcct cggtattgat gcgctggtcg ttctcggcgg cgaaggcact
     2041 ctcggcattg cagcagaatt tgaccaaaga ggaattcctg tctgcggtgt tcccaaaacg
     2101 atcgacaacg acctcgcttg cactgaactt acctttggat ttgtaaccgc actcgacatc
     2161 gcaacggaag cgctcgatag attgcatacg acagccgaat cccacgatcg agtaatgata
     2221 cttgaggtca tgggccggca tgccggttgg attgcattac attcgggaat tgccggcggc
     2281 gccgatgtca tcctgatacc tgaaatcccc ttctctcgcg aggccgttgc tgagaagatt
     2341 agagttcggg aaaatgcggg gaaaaatttt agtatcatcg tggtagctga aggcgccgtc
     2401 gaaattggtc accagttgat ctatcaagat cgcggagatg ctcggcatgc accacgactt
     2461 ggaggaatag gcgtccatct ccaacgagac ttagaagctc tgacagaacg tgagacgcgt
     2521 tgcgtagtcc tgggccactt acaaagagga ggccgcccaa acgctttcga tcgaatgctt
     2581 gctacgaact tcggttcgtg tgccgtaagg gcgctgtcac aaggcgaaaa aggagtgatg
     2641 gtggcattac aggcgggcga tgtagttaca gtaccgctta ccgaagctat taaaaacatc
     2701 aaatcggtcc ccccaaacgg ccaattggtc cggaccgccc gagatacggg aatttctttt
     2761 ggctctccgg acgaagccaa ttatcatgag taagaattga agaagaaaga gagaaagtcg
     2821 ttgtcacaac aaggtatcaa ggacaagctg cagggcgaat tggacgaact ggaatccgag
     2881 ttgcgggttc atctgccgaa ggagattaag cgagcgctgg agttcggcga ccttcgtgaa
     2941 aactcagaat atcgagcagc tctcgacagg cagaacgttg taaaagctcg tattgttgaa
     3001 cttcgacaac gtatcagcga gattgcgtcg atcgatccga ataaagttcc acgcgataaa
     3061 gcgtcttacg gctctacact cgtactttac gacgctgaaa aggacgagga agtaacctac
     3121 cgtctcgt
//
LOCUS       DASSFP010000236         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2147560, whole genome shotgun sequence.
ACCESSION   DASSFP010000236 DASSFP010000000
VERSION     DASSFP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2147560"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            591..1226
                     /locus_tag="VFH91_05165"
     CDS             591..1226
                     /locus_tag="VFH91_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET6862410.1"
                     /translation="MIGIAIAFLFGLATTVYLSLRSPEVKVPDVVGKNRFDAEKILAG
                     ADLNFRVRATRPSNQAQPDTVLFQLPRPGEVVKTGQTVAMDISRAAKEGETPETVKPE
                     EESSENSNSNANTATNLNENKPKKPKNKNANDNLNGNANSNANVNRPVNANRTPNANR
                     PNVDATPDNSNTIPRTNINRVNSNTRTNSNQNENRRPIAPKPSPGGVTPPN"
     gene            1249..1923
                     /gene="rpe"
                     /locus_tag="VFH91_05170"
     CDS             1249..1923
                     /gene="rpe"
                     /locus_tag="VFH91_05170"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET6862411.1"
                     /translation="MSNRTVDIAPSILAANFSCLGDEIRAAERGGAAILHVDVMDGHF
                     VPNITVGLPVVKSLSRFTKLPIDAHLMISEPGIYATQFVEAGARMVSVHVEADPNLHR
                     TLTAIRSAGAHPGVVLNPATPLSAIEEALRFVDYVLVMSVNPGFGGQKFIPQTLYKVK
                     RLRQLIDERQLKVRIEIDGGVDRNNIETVVAAGAEIIVAGSAVFGTGDAENAVKELRN
                     ATVQWV"
     gene            2035..3576
                     /gene="bamD"
                     /locus_tag="VFH91_05175"
     CDS             2035..3576
                     /gene="bamD"
                     /locus_tag="VFH91_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET6862412.1"
                     /translation="MRSRFYALISLCALMIGLSQIVLAQRQGGPAELSDAQRLDVMTS
                     KLEQMRRSLNSALSAMAPADKSKDNKDKKANPDDPAVRLKNLEKDVSSLMSEVNDLRT
                     KNDKAEKFDPTNLDRLEAAVTEIGPRVDSALQQTASAKNAAGSSAASNSKKKKKGFLG
                     LFGGSDDKYAELTGAAVAGRDRVLFEEAAKEVRKNNYDTGRLLFSTIINTYPDSPFLS
                     LAKLAIADSFFLEGTTSALIQAAQAYQDWLTFFPTHPLADDAMLKVAESEMRQMGLPD
                     REITHAKKAEVRLKVLLQQYPKSSLRPVVEDRLREVQENLAMHNLAVARFYQDARFTQ
                     GKGGLKGAQGRLKEIVTNYPCFSYNDEVLFRLGSTYQAEEEPDEAAKYYQQLVQQFPQ
                     SQFNDKARDQLNIIGAPIPEKVTPNECAKKSQLGFVGSLMQQVSGKAEVSFNKDGVLI
                     SKGGKEGTDLIDEALKYNGELPENRIPVAPVQRNTSRTQADPKGKNANNVAPSNSATP
                     AAVKP"
     gene            3699..4193
                     /locus_tag="VFH91_05180"
     CDS             3699..4193
                     /locus_tag="VFH91_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862413.1"
                     /translation="MDSLPPNVREELIKRHKAASVTIVGLVVAILLLSIIAYLARPYF
                     RQQVDSSIDLAVRLVVFVLGAGSIVWRRTKFATMRLQDIGSLQGALGVMKTLERTTIQ
                     VALLSEGVAVSGFLATAMTGNALYSYWSGVIAIILLVYHYPRRASWNNALLKYAGDLN
                     SAPA"
BASE COUNT         1223 a         1116 c         1210 g         1135 t
ORIGIN      
        1 taactaataa taaccggcaa gttggatgta ctgattggca ggctttcact ccggaagagt
       61 gacatggtta tagacttggt cctacttctc ctctcctcca ctccgttcga agttctaaga
      121 aggagcggtg atgatcttga agcgtacagt cctagtcgct ccttcttaga acttcgaacg
      181 gagtggagga gaggaggagt acggccaagt ctataaacat gtcgctccta cggagtgaaa
      241 ccctaccaat ctatcaatca atcaatacat ccattttgcc tgttattatt agttacgttt
      301 gcgcgacgac gaccctgcag tttaccaaca tgttcggcaa actgtttttt tcttaacgaa
      361 ctttagaaac aggacgctat ttagaattgg acttacttag aatcggagat cttagggggg
      421 acggggccta aaaagcgccg ccgggccggc gcactccaaa gttgccactc gctagggtgg
      481 gtgttagact tcgcaacttg gtgttgaccc tgtagttgtt ccccgatgat agacattaag
      541 cattgagcgc agcttccagc atcctttctg tttttagaag aatttcaatc gtgatcggca
      601 tcgcgattgc ttttcttttt ggtctagcca ccaccgtcta cctttcgttg cgaagccctg
      661 aagtgaaggt tccggatgtg gtaggcaaga accgtttcga cgcggaaaag attcttgcag
      721 gtgcggatct gaattttcgt gttcgcgcga cgagacccag taatcaagca cagcctgaca
      781 ctgtattgtt ccaattgccg agacctggtg aagtagttaa gactggacag actgtcgcaa
      841 tggacattag tcgggccgcg aaagagggtg aaactcccga aactgttaag cctgaagaag
      901 agtcaagcga gaacagcaat tcgaacgcta atacagcgac gaacctgaac gaaaacaaac
      961 ctaagaagcc gaagaacaaa aacgctaacg ataacttgaa tggcaacgcg aatagcaacg
     1021 ccaacgtcaa tcgaccagtt aacgccaatc gcacgccgaa tgctaaccgg ccaaatgtgg
     1081 atgcgacgcc agataattcg aatacaattc cccggactaa tattaaccgc gtgaactcta
     1141 atacccgcac caatagtaat cagaatgaga atcgccgccc cattgctccg aaaccgagtc
     1201 cgggtggcgt aacgccgccc aactgagcag agcgttagtc aaagtttgat gtcgaataga
     1261 actgtcgata tagctccttc aatactggct gcaaatttct catgcctggg tgacgagatt
     1321 cgggcagccg aacgaggagg agctgcaatt ctccacgtgg atgtgatgga tggtcatttt
     1381 gtaccaaaca tcactgtcgg attgccggta gtgaagtctc tatcgcgctt tacaaagttg
     1441 ccgatagatg cgcatttaat gatttctgaa cccgggattt atgccaccca gttcgtcgag
     1501 gctggagcac gaatggtttc cgtccacgtc gaagcagatc caaacttgca tcgtaccttg
     1561 acggccatta ggtccgctgg agctcacccg ggagtcgtgc taaacccggc tacacctttg
     1621 agtgccatcg aggaggctct gcggttcgtc gattacgtac tggtgatgtc ggtgaatcca
     1681 ggttttggcg gccagaaatt cattcctcaa actctgtata aagttaaacg actgcgtcag
     1741 ttaattgacg agcgccagtt gaaagtacga attgaaatag acggaggcgt agatcgcaac
     1801 aatattgaaa ccgttgtcgc agccggtgct gagataatcg tcgccggttc ggcggttttc
     1861 gggactggtg atgcggagaa tgccgtcaaa gaattgcgca acgccaccgt acaatgggtc
     1921 tgaaaagctg tttttataat actttgcgtg gttcggcgaa ccgcaaaggt gaaatttgaa
     1981 tccgatagcc gtaactcttt ctcgcccgaa gttaagtttt tggaggtttt ccttatgagg
     2041 tctcgctttt acgcacttat ctccctgtgc gctttgatga ttgggctatc acaaatagtc
     2101 ctagctcaac gccagggcgg gccggctgaa ttatcggatg cgcagcgcct ggacgtcatg
     2161 acctccaaac tggagcagat gagacgttca ttgaatagtg cgctttcagc gatggctcca
     2221 gctgataaga gcaaagacaa caaagacaaa aaggcaaatc ctgacgatcc tgccgtccga
     2281 cttaaaaatc tcgaaaagga cgtttcgtcc ttaatgtccg aggtcaacga ccttcgaacc
     2341 aagaatgaca aggcagaaaa gtttgaccca accaacttag atcgacttga agcagccgtc
     2401 accgaaattg gtccgcgcgt cgattcggcc ttgcagcaaa cggcaagtgc aaaaaatgca
     2461 gccggcagta gcgctgcaag caacagcaaa aagaaaaaga agggttttct tggtctattc
     2521 ggcggcagtg acgacaaata cgctgaatta acaggagcag cggttgccgg tcgcgatcgt
     2581 gtgctgtttg aagaggcagc caaagaagtt cgcaaaaaca actacgacac cggacgactg
     2641 ttgttttcca cgatcattaa tacctatcct gattcaccct ttttatcgct ggcaaaactg
     2701 gcgattgccg actcgttctt tctcgaagga acaaccagtg cgcttatcca ggcagcccag
     2761 gcctatcagg actggctgac gttctttccg actcatccgc tggcagatga tgcaatgttg
     2821 aaggttgctg aatctgaaat gcgtcagatg ggtcttcccg atcgtgagat cactcatgcg
     2881 aagaaggcag aggtacgtct caaggtattg ttgcagcaat atccaaagag ctccttaaga
     2941 cccgtggtcg aagatcgtct acgtgaagta caagagaacc tggcgatgca caatttagcc
     3001 gtcgcgcggt tttatcagga tgccagattc actcaaggga agggtggcct taaaggtgca
     3061 cagggccggc ttaaggagat agtcacgaat tatccgtgct tctcttacaa cgacgaagtc
     3121 ttgtttagac ttggtagcac ctatcaggct gaggaagaac cggacgaggc tgcgaaatac
     3181 taccaacagt tggtccaaca atttccccaa agtcagttca acgacaaggc ccgcgaccag
     3241 ttgaatatca ttggcgctcc gattcctgag aaagttacgc cgaacgaatg tgcgaagaag
     3301 tctcagttgg ggtttgtggg aagcctgatg cagcaagttt cgggtaaagc tgaagtgagc
     3361 tttaacaagg atggcgtcct tatcagtaag ggcggcaaag aaggcaccga tttgattgac
     3421 gaagctttga aatacaacgg agagctcccc gaaaatcgta ttccggtggc tccagtacag
     3481 agaaatactt cacgcactca agccgatcct aaaggcaaga atgcaaacaa tgtggctccg
     3541 tctaactcag ctactccagc ggcagttaag ccctgatcag gagatttcca gttttcgaag
     3601 aggcccgctg ttcaaaaccg gtgggccttt ttgctttcca cttctaatga tgactggtat
     3661 tttgtgagac aattccagtc caaaattgga gcctgttaat ggactcgttg ccgccgaatg
     3721 ttcgagagga acttatcaag aggcacaaag ctgcctcagt aacgatcgtt ggattggtcg
     3781 ttgccatttt gcttctctca atcattgcct acctggcgcg cccctatttt cgtcaacaag
     3841 tagattcctc gattgattta gctgtgagac tggtcgtgtt tgtgcttggc gccggatcga
     3901 ttgtttggcg gaggacgaag tttgcgacga tgcggctgca ggacattggg tcactccagg
     3961 gtgcgctggg agttatgaag actctggagc ggacaacaat tcaagttgca ctgctgtctg
     4021 aaggggttgc agtcagtggt tttttagcga ctgcgatgac gggtaatgcc ttgtacagtt
     4081 attggtcggg agtgattgca attattttgc tggtctacca ttacccgcga agagcaagct
     4141 ggaataacgc ccttctcaaa tacgccggcg atttgaattc cgcccccgcg tgagtaagct
     4201 taaggtccaa cctacaaaaa ggcgcccccc ggacttccgc gaattccacc aacggccgag
     4261 accgtgtaga aactcaaata ccacccacgt gagtgggtgg atggttcaga tcctttctac
     4321 aactagccgc cagaggtttt ggaaatacca cccacgtgag tgggtggatt tgttcaggtc
     4381 ctcgctaacg tggtgagagc gttttgaaat ccagtcaacg gcgggtttag ttaaaaaaag
     4441 atccttaatt ggtggcctct tcgtagaaag gatctgaacc atccacccac tcacgtgggt
     4501 ggtatttcgt taggaacgtt ggcctctttg tagaaaggat ctgatcgaca taggtaggct
     4561 gtcaccagct ttccctgtca caccaccgtg catacgggtc cgtacacggc ggttcggcga
     4621 gtgagactta ggtaacgact aacggcgtca gtccgagcga tgcaaagtat gcgttgggca
     4681 gtgc
//
LOCUS       DASSFP010000237         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2004109, whole genome shotgun sequence.
ACCESSION   DASSFP010000237 DASSFP010000000
VERSION     DASSFP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004109"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFH91_05185"
     CDS             <1..238
                     /locus_tag="VFH91_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6862414.1"
                     /translation="QGSALRIVDQFEQSVASPRTLEEVEKEYITRILDETSWRVEGTN
                     GAAKILGLNPSTLRTRMAKLGVLRRFHVAGGRNH"
     gene            642..2645
                     /locus_tag="VFH91_05190"
     CDS             642..2645
                     /locus_tag="VFH91_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET6862415.1"
                     /translation="MFSLSFRQPRENSRRYFAAARLRNIGSLLICSLLVLGSVLPFCP
                     VSRAEIPTPSRQSSRKQIPLPTPLSHYETFKPGTSGLPLLLDLLKEFEPETQKVLLRF
                     ALASGLQDIAAHPELASADLKKIQSLAEEAGLPKAPPIVKEQAIALIKQTNWAAHRPI
                     LLEFFLHQSQVLEMIPEKWGSIWVPIVHDALLYFLDHLSDDRLLDKLVSLALLPPGSS
                     RGDYLKEFVSKVPSLQKLGQILARNPDLSPDYRKALQDLENGIHTMTGEELVTFITDD
                     VGKPSIDKYQVQFADHILAEASVGAVIRASTIPPGGTVRRQAICKVVKPYVLVYMPED
                     LSIIEGLASYFTVNHDFYQLGSMPLVEVFQELGKSLADEINITDEQKNFIRAREYYKG
                     SKKVVVPEIFPISTKHVTFMEFIAGQKITSAFPGDTQKRAIMARRLSDVMTGDVIFSP
                     RSEAIFHGDPHPGNVYHITGNPKNPYLIGLLDWGLMGTFPRKDRVALMQLILGVQLGD
                     AKRLHNNVGALLDHGLPDSAEKVQKIDALIAKVIIPKPGRGSFDALEELLFGLIEQGY
                     ATKFTLNIFIKSQITIAGELVELDPTLKQDDLLEKQVTALVKKEMPKRLLCTAWFPCW
                     DSHGYQSLLSNGDVMDARRIKKPKESKTPPPKTVAVPKVQKQH"
     gene            2782..3501
                     /locus_tag="VFH91_05195"
     CDS             2782..3501
                     /locus_tag="VFH91_05195"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HET6862416.1"
                     /translation="MQKYCTSALAIPVLVLIIAGQGSSQTSSAATEVWPSVQVSFDPQ
                     PRIGVQLLFEKHNDEDVSRAQWKISAIASYRMKRILNRHRDDIDPENEYNLAVGGGYE
                     YVRTEQTSGNKIEHRVIFQTIPKYVPFWGILVQDRSRAEFRWVDDVYNFRYRNRLTVD
                     REFKLNKVRLTPYVSGELFWDRNHHAWTENQYKFGVQLPYKKLLMLDTYYLRQNFTTC
                     SQDPLSVFGIRLNLYFKRKRK"
     gene            3745..4257
                     /locus_tag="VFH91_05200"
     CDS             3745..4257
                     /locus_tag="VFH91_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6862417.1"
                     /translation="MKTMRIVLGLSLLLLATNAFGQKVTTDSVPNTDWSKYHTYSWGE
                     GTPAQDPITAQRIVAGIDSQLAAKGLKKVDADPDLVVIYHTSTAQQKSLNWSSMGGFG
                     RFGGMGSAQVDTVINGQLKVDIADYKAKQFLWRGTATDTVSDNPQKVAKTLNKALTKM
                     FQKFPPPPGQ"
     gene            4308..>4855
                     /locus_tag="VFH91_05205"
     CDS             4308..>4855
                     /locus_tag="VFH91_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862418.1"
                     /translation="MRITIRKAILVTAGMSLMFAVLLTSARTSAQDDTKLPKSIFIQG
                     EAMGQSTQMGQMFSLNLIIEEFSSADDQKVLVDAFNTKQNEGLVNALSKMKSKGRMAI
                     TGTLGYDVNYIRKFDQPDGSTKIRLVTDRPITFGEAWTDSRSSDYNLSGMEIILSPDK
                     KKNSGLLAPACKFKIDKENQLQL"
BASE COUNT         1273 a         1214 c         1197 g         1171 t
ORIGIN      
        1 gcagggatcg gcgctgcgca ttgtcgatca gttcgaacaa tctgtggcat cacctcggac
       61 tcttgaggag gtcgaaaagg aatacataac ccgcatactc gacgaaactt cctggagagt
      121 agaaggcaca aacggcgctg ccaaaatcct cggccttaac ccaagcacct tgcgtacacg
      181 gatggcgaaa ctgggagtgc tgcgacgatt tcacgtcgcc ggcgggcgca accactaggt
      241 cttagtccag gcttgaattt gatgtcgagc ccgcaatgag ggcagtaaag actatccgca
      301 gattacgccg attacacaaa tttcaacctc aaatggtgcg actaactcgt ccgcgtttag
      361 aaaaaaccgg cgtaatcagc gcaatccgtg gatgattctt ttaggtctct tgtaaatcac
      421 cttcggtagc ggagtgacgt tggataatca ctgcgaggta ctggcagatt tgacagtaac
      481 tttgcctacc ccgtaagtga taccgtcaat ctgtatattg cctggctgtg ttggttcggg
      541 cggttgtaaa cggccgggct tgaggttgtt ctatttttct tacgccatca atgcgtgagt
      601 ggtgataagg aagccttccg gagtaaggca aggaggatct aatgttttcc ttatcgttca
      661 gacaaccgcg agaaaattcg cgtcgatact tcgccgctgc aaggttacgt aatattggct
      721 ccctattgat ttgctcgttg ctggttctcg gatccgtcct gccgttttgt cccgtttcgc
      781 gagcagaaat tccaacgcca agccggcaaa gctcccgaaa acagattcct cttccgacac
      841 cactaagtca ctacgagacg tttaagcccg gcacttcggg gcttccacta ttgctcgact
      901 tattgaagga atttgagccg gaaacacaga aggtactttt gcgttttgcc ctcgcctcag
      961 gacttcagga catcgcagca caccctgaac tcgcatccgc agaccttaag aagatccaga
     1021 gtctggccga agaagctgga ttgcccaagg caccgcccat tgtgaaagag caagccatcg
     1081 ctttgatcaa acaaacgaac tgggctgcgc atcgtcctat attgttggag ttctttttgc
     1141 atcagtctca ggttctggaa atgattcccg aaaagtgggg aagcatctgg gttcctatcg
     1201 tccatgatgc gttgctctat tttttggacc atctttccga tgatcgtctt ctggacaagc
     1261 ttgtcagcct ggccttgctt cctccgggtt cgtcgcgagg agactatctc aaagaattcg
     1321 tttccaaagt cccaagcctg caaaagttgg gtcagatttt ggcccgcaat cctgatctgt
     1381 cgcctgacta tcggaaagcg ctgcaggatt tggaaaacgg cattcacacg atgactgggg
     1441 aggaactcgt cacgttcatt actgatgatg tcggcaagcc atcgatcgac aaatatcagg
     1501 tgcagtttgc tgatcacatt ctcgccgagg cgagcgtggg cgccgtcatt cgcgcctcaa
     1561 caattccgcc cggaggcacc gtgaggcgtc aggcgatctg caaagtcgtt aaaccttacg
     1621 tgctcgtgta tatgccagag gatctttcca ttattgaggg actggcatcc tacttcaccg
     1681 tcaatcacga tttctatcag ctgggatcga tgcctctggt agaagtcttc caggaactcg
     1741 gaaagtcctt agctgacgaa atcaacatta ctgacgaaca gaaaaacttc atccgtgcac
     1801 gcgagtatta caagggtagt aagaaggttg tcgtgccgga gatatttcct atctcaacaa
     1861 agcacgtcac gttcatggag tttattgctg gtcagaagat caccagcgct ttccctggtg
     1921 acacgcaaaa gcgagccatc atggcaaggc gattgagcga cgtcatgact ggcgacgtca
     1981 ttttttcgcc cagatcagaa gccatctttc acggagatcc gcatcccggt aatgtctacc
     2041 acatcactgg taatcctaag aacccgtact tgatcggact cctggattgg ggcttgatgg
     2101 gaacatttcc gcgtaaggac agagttgcac taatgcaatt gatcctcggt gttcagctcg
     2161 gagatgccaa acgtttgcac aacaatgtag gggcgttgct ggaccatgga ttacctgatt
     2221 ctgcggagaa ggtgcagaag attgatgccc tcatcgcaaa agtcatcata cccaagcccg
     2281 gacggggcag ctttgatgcg ctggaagaat tgctatttgg attaattgag cagggctacg
     2341 caaccaaatt cacgctgaat atctttatca aatcgcagat tacgattgct ggagaacttg
     2401 ttgagttgga tccaaccctt aagcaggatg atcttctgga gaagcaagtc acggcgctcg
     2461 tcaagaagga aatgcccaag cgattactct gtacggcctg gtttccctgt tgggactctc
     2521 acggctatca aagcctgctc tccaacggtg acgtaatgga tgctcgtcgc ataaaaaaac
     2581 caaaagagtc aaagactcct cctccgaaaa ctgttgcggt gccgaaagtt cagaaacaac
     2641 actgaatacc tcttggaacg ctgttgccga tgaatctcag gatgactctc tgctcttgtg
     2701 cttggattaa ttgtcgcgtc ggcaaaagga acgtttgatc agttaagaac gaactaatga
     2761 ctgcagactc acggaaatct catgcaaaaa tactgcacat cggctcttgc tatccctgtg
     2821 ctagtgctta tcatcgccgg acaggggtct agtcaaacct cgagtgccgc aaccgaagtg
     2881 tggccttccg ttcaagtatc ttttgatccc caaccgagaa tcggtgtcca actgctcttc
     2941 gaaaaacata acgacgaaga cgtttcacgg gcccaatgga aaattagtgc gatcgccagc
     3001 taccggatga aacggattct aaacagacat agagacgata tagatcctga gaacgaatac
     3061 aatttggcag tgggcggagg atacgaatat gtccggaccg aacagacgag tggtaacaaa
     3121 atagagcatc gagtgatctt ccaaacaatc cctaagtatg ttcctttttg gggaatcctg
     3181 gtacaggatc gcagtcgtgc cgagttccgt tgggtggacg acgtctacaa ctttcgctac
     3241 cgcaatagac tgaccgtgga ccgagaattc aagctcaaca aagtgcgtct aacaccttat
     3301 gtttcaggcg agctcttttg ggatcgtaat caccacgcct ggactgaaaa ccagtacaag
     3361 tttggcgtcc aactgccata caagaaactc ctaatgctgg acacttatta cttgcgtcaa
     3421 aattttacga cgtgcagtca ggacccgttg tccgtcttcg gcattaggct caatctttat
     3481 ttcaagcgga agcgaaagtg atcaaataca ctgtcaaaat cgacactcaa actatagcaa
     3541 gcctctctcg caactaatcc agccattggg cttaccgacc ctcttcaagc gccgtcaaaa
     3601 actgttagaa ctgacagtta ctttgcgaac tctatctgca tagaattcac caacacttga
     3661 atctttgttc tgatgcaact ggttgtgatc agagaaacgt ccccgccgaa agcgcgaggc
     3721 cattaagttc gaaggagaag tactatgaaa acaatgcgca tcgttcttgg cttgtcgctt
     3781 ttgttgctgg ccaccaacgc gttcggacaa aaagttacga ccgattcggt tcccaatact
     3841 gattggtcca aatatcacac ttactcgtgg ggcgagggga caccagccca ggacccgatc
     3901 actgcgcaac ggattgtggc cggcatcgac tctcaacttg ccgcgaaagg tctaaagaag
     3961 gtggatgccg atcctgatct cgtcgtgatc tatcacacgt ccactgccca gcagaaatcg
     4021 ctcaactgga gcagtatggg cggctttgga cgctttggtg gaatgggctc cgctcaggta
     4081 gataccgtta tcaatggtca gttgaaggtg gatatcgccg attacaaagc gaaacagttt
     4141 ctctggcgtg gtactgcaac cgacacggtt agcgacaatc cgcaaaaggt ggccaagacg
     4201 ctcaataagg ctctgaccaa gatgtttcaa aagtttccac ccccgccagg ccaatagtgt
     4261 cgggcacaca tcccccgcca ccgtagcgtt aagtggagga cagtcaaatg cgaatcacaa
     4321 ttaggaaagc gattcttgta acagccggaa tgagtctgat gttcgccgtg ctgttaacaa
     4381 gtgccaggac atcagctcag gatgacacga agttgcccaa gtccatcttc attcagggag
     4441 aggcaatggg ccagtcgacc caaatggggc agatgtttag tttgaatttg ataatcgagg
     4501 agttctcttc agccgatgat caaaaagtcc ttgttgatgc gttcaatacg aaacagaatg
     4561 aaggcctggt caacgctctt tccaagatga agagcaaggg tcgaatggcg atcaccggca
     4621 ctctgggcta cgacgtgaat tacatcagaa agtttgacca acctgacggc agtacgaaga
     4681 ttcgtctggt aacagatcgt ccaattactt ttggcgaagc ctggaccgac agccgatcat
     4741 cggactacaa cctatcaggt atggagatca ttctttcgcc tgataagaaa aagaactccg
     4801 ggcttctcgc gccggcttgc aagttcaaga tcgacaaaga gaatcagctt cagct
//
LOCUS       DASSFP010000238         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2148229, whole genome shotgun sequence.
ACCESSION   DASSFP010000238 DASSFP010000000
VERSION     DASSFP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148229"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1307
                     /locus_tag="VFH91_05210"
     CDS             234..1307
                     /locus_tag="VFH91_05210"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET6862419.1"
                     /translation="MSSHQSANNQIPLAEPLVEVRRGGLTESRHRGHIVAVEPNGRIV
                     KYLGAPENVTFLRSSAKPFQALPLLVSGAAEHFDYNDREVALACASHNGEPIHTELAS
                     SMLKKINLGPEALKCGIHEPYSQAVARELRASGVRPNVLQNNCSGKHAGMLALAKHLG
                     APTENYDSPESPVQIGVARTIASFSGVPIQDLAVAIDGCGVPVFGITVKAMALAFAKL
                     VSPSSEFDETTRLSCHRIVKVMTAYPELIGGTRDRLDTEIMRSSPGRIVSKVGAEGVY
                     TAGILPCEEWPNGLGLALKIEDGDDHRARPTVVIEALRQLGVLRDESLEFVSRYAFFP
                     IKNRRDEVVGEIRASFKLKENSN"
     gene            1311..1853
                     /locus_tag="VFH91_05215"
     CDS             1311..1853
                     /locus_tag="VFH91_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862420.1"
                     /translation="MSQPSPDKANELVQEPPLVRDSNGQVILDSVPDVIEWFLNYDQR
                     VAVIRHRSVEEVFQWKQQQSKASGEEIFMFQKAEDRLAIGIIQALGENGTERELHLWI
                     SQLLNALDIAAKANEATAEAYKLDLKNSESIVSKAELIPFERGRNDFLINCWIETLCT
                     AEARVLGWLYKELYGHPFTP"
     gene            1875..2999
                     /locus_tag="VFH91_05220"
     CDS             1875..2999
                     /locus_tag="VFH91_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6862421.1"
                     /translation="MTPELRALFPITQRAVYLNHAAVSPLPVPTIEAVESQLRDVHKN
                     GSLNFRDWLGVKQSARVLLAELLNARPEQIAFTRNTSDGLSTVANGISWQPGDNIVTF
                     KHEFPSNIYPWLRLTQSLGVELRTCNEHDGRIDLDELTRLVNQHTRVIAISHVQFASG
                     FRVDLERLGKLARKHDALLVVDTIQALGVIPTDVEAELIDVAAGGGHKWLLSPEGTGY
                     LYLSDRARERIEPTLVGWVSVPNPEDHFNWNQGWNDGALAWETGTGPAALIYGLEASL
                     RLIRYQGVGRIQKYLQELTDYLCSQLDLRNYEIVSSRLKNEQSQILCIRHTGGMGAMD
                     LYSRLKQRRIITAPRVDRLRIAPHFYNTFEDIDALISALP"
BASE COUNT          836 a          801 c          785 g          733 t
ORIGIN      
        1 agagacgtgc ggcccatcca gcgaagaaat aacttaaaac agactaaaac taaacagagc
       61 cgaggcagaa ggtttttata cctgctgcct tttttgtttg tgggagaagt gactcttgcc
      121 cccttcccaa ctgcgtcatt ctggtaaccc tctgagatct aaagataatc ggtcgacgtg
      181 ataacatcat cagtcttgaa aagaaatctt ttatttcgac tccactgacc ataatgtcat
      241 ctcatcagtc tgcgaataat cagataccac tcgctgaacc attagtggaa gtccgacgag
      301 gagggctcac agagtctcgt catcggggac atatcgtagc cgttgaaccg aacgggcgaa
      361 ttgttaagta cctcggcgct cctgaaaacg tcaccttcct gcgctcgtcg gcaaaaccat
      421 ttcaggcttt acctctgctg gtttccggcg ctgccgagca ttttgattac aatgaccgcg
      481 aggttgctct agcttgcgcg tcgcacaatg gagagccaat ccatactgag ctcgcctcgt
      541 cgatgttaaa gaaaatcaat cttggacccg aagccctaaa gtgcgggatc cacgagccat
      601 atagtcaggc agtagcgcga gaactccgag ccagtggcgt gcgaccaaac gttctgcaaa
      661 acaactgttc cggcaaacat gcgggcatgc tggcactcgc gaaacacctc ggtgctccga
      721 ctgaaaacta cgacagccca gagagcccag tacaaatcgg cgtggcgcga accatagcga
      781 gcttctcggg agtacccatc caggatctcg ccgtggcaat tgatggatgc ggcgtgccgg
      841 tgtttggaat caccgtcaaa gcgatggcac tcgcgtttgc aaaactggta tcgccttcgt
      901 cagagtttga tgaaacgacc cgactttcat gtcatcggat tgttaaagtt atgacggcct
      961 atccggagct tatcggcggt accagggatc gactggacac agaaatcatg cgctcgtcgc
     1021 ctggtcgaat tgtttccaag gtcggagcag aaggcgttta cactgccggc atcttaccgt
     1081 gcgaggaatg gccaaatggc ctgggcctgg ctttgaagat tgaggacggt gacgaccatc
     1141 gagctcgccc gacggtggtg atcgaagctt tgcgccagct tggcgtccta agagatgaat
     1201 cactagaatt cgtctctcgt tacgcattct tccctattaa gaacagaagg gacgaagtcg
     1261 ttggtgaaat cagggcctcg tttaaactga aagagaacag caactagaga atgtcgcaac
     1321 catctccaga taaagccaac gaactagttc aagagcctcc tctcgttcgt gactccaatg
     1381 gccaggttat attggactcg gtacctgatg tcattgagtg gtttctaaat tacgatcagc
     1441 gcgttgcggt cataagacat cgatccgtgg aagaagtttt ccagtggaaa cagcagcaaa
     1501 gcaaagcatc gggcgaagag atcttcatgt tccaaaaggc tgaagatcga ctggcaatcg
     1561 gaatcatcca ggccttggga gaaaacggca cagaacgcga gcttcatttg tggatcagcc
     1621 agttattgaa tgcactggac atcgcagcca aagctaacga ggccacggcc gaagcttata
     1681 agctggacct taaaaactca gagtcaatcg tttctaaggc tgaactcatc ccctttgaac
     1741 gagggcgtaa cgatttcttg attaactgtt ggattgaaac tctttgcaca gctgaggctc
     1801 gcgtgctggg ttggctgtac aaggaactct acggtcaccc gtttactcct taatccttaa
     1861 gccttcaaat acttatgaca ccggaactgc gcgcactatt tccgattacc cagcgcgctg
     1921 tatatctgaa tcacgccgcc gtatcgcctc taccagttcc aaccatcgaa gctgttgaat
     1981 cccagcttag agatgttcac aagaatggct cgttaaattt cagagactgg ctcggagtta
     2041 agcagagtgc aagagtcttg cttgcagaac tccttaacgc ccgccccgaa caaatagcat
     2101 ttactcgaaa tacatcggat ggtctctcca cagtcgcaaa cggaatcagt tggcagccag
     2161 gtgacaacat cgtcacgttc aagcatgaat ttccttctaa catttatccg tggctgcgat
     2221 taacacaatc gcttggagtc gagctgcgaa cttgtaatga gcacgacggc cggatagatc
     2281 tcgacgaatt gactcgcctc gtaaaccaac acacacgagt aatagcaatt agtcatgtac
     2341 agtttgcgtc cggatttcgg gttgatcttg agcgcctcgg aaaactcgcg cgaaagcacg
     2401 atgcattgtt agttgtagat accatccagg cacttggcgt gattcctacc gatgttgaag
     2461 ccgagttgat cgatgtggct gcgggaggcg gtcacaagtg gctcttgagt cctgaaggca
     2521 ccggctatct ttatctgtca gatcgagctc gcgaaagaat agaaccgacg ttggtagggt
     2581 gggtaagcgt acctaaccct gaagaccact tcaattggaa tcagggctgg aatgatggcg
     2641 ccctggcctg ggaaactggc accggtccag cagccttaat ttacggcttg gaagccagcc
     2701 tccgattgat cagataccaa ggggtaggtc gcatccagaa ataccttcag gagctcaccg
     2761 attacctttg ctcccaactc gacctgcgaa actatgagat cgtgagttca cgattgaaaa
     2821 acgaacagtc gcaaatcctg tgcatccgac atactggagg catgggagcg atggatctct
     2881 attccaggct gaaacagcga agaataatca ccgcaccacg agtggatcga ttgagaattg
     2941 cccctcactt ctacaatacc ttcgaagata tcgatgctct gatatctgcg ctgccctaat
     3001 cacgcacact cttccaagcg atccgagcga ttatcggtcg gttgtactgc cgattcgtac
     3061 gaaaattgta cgcaaggcga agagaaagct agactgcgat ctcgcctgaa tttgcaaaag
     3121 agtcccacaa aatcggtgaa agttaaattt cgatc
//
LOCUS       DASSFP010000239         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_802492, whole genome shotgun sequence.
ACCESSION   DASSFP010000239 DASSFP010000000
VERSION     DASSFP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802492"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VFH91_05225"
     CDS             <1..1401
                     /locus_tag="VFH91_05225"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6862422.1"
                     /translation="LLPTNFAAGAPTQQTAPKTAPTRQTTAAASQAKFPEAKIISQAL
                     PNGLEVVIMEDHSIPLVTIELAVKNGSYTEPPELNGLSHLYEHMFFKQNRAIANAEEY
                     LRAIGQMGIAYNGTTREEVVEYYFTTTSPNLRTAMQFMKDAARYPLFDEREFAREQQV
                     VIGEIDRNESNPFFPLQQEMNNRLFYRYTSRKNPLGNRQTVSGATTAMMRLIQQRYYV
                     PNNSALIVTGDVEPQEVFKMAQEFYGDWARREKDPFVEFPLVEHPQLAKSEGSVITSP
                     VQNVVINIGWHGPSIGKDNPATYAADVFSFILRQPNSRFQRNLVDSGLVTGVDLSYYT
                     QRNVGPIQLLAQTTPDKAHAAIKAIYDEIAHFNDKDYYTDEQLESAKALLEADDLYSR
                     EKTSDYTHTLGFWWASTGLEYFRGYLPRLRATSRADISRYVSTYIQGKPHVGLALMSS
                     DAQAKIKVQPEELIGQ"
     gene            1417..2844
                     /locus_tag="VFH91_05230"
     CDS             1417..2844
                     /locus_tag="VFH91_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6862423.1"
                     /translation="MNEITKSLMSVRNLIITVVAVSLLVLGNGDSTLASGQQGRLTPK
                     PEQQTVQSVVAKQAALVSEFEVNGLKVLLKRREGSLTVAAGLFLRGGAANTNAGNAGI
                     EQLMLNASTEASTGFPREKMRSELSRMGTTIGTGSNYDYSVLALACTRLHFDRSWQIF
                     SDVIMRPSFTTEDVALDRDRMVVSLSDDIDSPDVYLQRLQDNVAYAGHPYLNSNGTPA
                     VIAKLSPDDLRRYHDKMMETSRLLLVIVGDLNVGDIRDLVASTVGKLPRGTYKPDPLP
                     SLSFDRSSVSVTPRDLPTNYVQGLFTAPSLTSPDIYPMRVASSMLRDRVFEEVRVKRN
                     LSYAPDAFLRTQAANVGGLYVTAVDANQSIRLMLNEIARLQHEPVDNDDIQSVVAQYL
                     TTYYLGQETNAAQAAELAQYELIGGGWRNSVDFLEKLTAVTPAEVQRVAQKYMKNIRF
                     VVLGNPRSVDSSIFTGAAAAAGVGR"
BASE COUNT          806 a          761 c          783 g          747 t
ORIGIN      
        1 ctgctgccca cgaattttgc agcaggagca ccaacccaac aaacagctcc aaaaaccgcg
       61 ccgacccggc aaacaactgc tgccgccagt caagcaaagt ttcctgaagc gaagattatt
      121 tcccaggcat taccaaacgg attagaagtg gtaattatgg aagatcattc cattcctttg
      181 gttacgatcg aactggcggt caaaaacggc tcatatacag aaccgcccga gttgaatggc
      241 ctctcgcacc tatatgagca catgttcttc aaacaaaatc gagcaattgc taatgcagag
      301 gaataccttc gagccatcgg ccagatgggt attgcataca acggtacgac gcgggaagaa
      361 gtagttgaat attactttac gaccaccagc ccgaaccttc gcaccgccat gcagtttatg
      421 aaggacgcgg cgcgatatcc gctgttcgac gaacgtgaat ttgcgcgcga gcaacaggtc
      481 gtaattggcg agattgaccg caacgagtca aatccattct tcccgctaca acaggagatg
      541 aacaaccggc tgttctatcg gtatacaagt cgcaaaaatc ctcttggcaa ccggcaaaca
      601 gtttcaggcg cgacgaccgc aatgatgcgg ttgatccagc agcggtacta tgttcctaac
      661 aactcggcgc ttatagtaac cggtgatgta gaacctcagg aagtgttcaa aatggctcaa
      721 gagttctacg gcgactgggc acgtcgtgaa aaagacccgt tcgtcgagtt tccactcgtt
      781 gagcatccgc agctggcaaa aagtgaaggg tccgtcatca cttctccagt gcagaatgtg
      841 gttataaaca tcggctggca cggaccgtct atcggcaaag acaatccagc aacttatgca
      901 gccgatgtat tctcattcat cctgcgacaa cccaactcgc gcttccaaag aaatcttgtg
      961 gactcaggtt tggtcaccgg agtggacttg agttattaca ctcaacgtaa cgttggtcct
     1021 attcagcttt tggcccaaac gacgccagac aaagctcacg ctgctatcaa agcgatctat
     1081 gacgagattg cgcatttcaa tgacaaggac tactacacag acgagcagct ggaaagtgcc
     1141 aaagcgcttt tggaagcgga cgatctgtat tcgcgcgaaa aaaccagcga ctacacccac
     1201 actctcggtt tttggtgggc ttctaccggg ctcgaatact tccgcggcta tctaccacgt
     1261 ttgcgtgcaa cttcacgcgc ggatatcagt cgctatgttt ctacctacat acagggaaag
     1321 ccccatgtcg gactggcgtt gatgtccagt gatgctcaag cgaaaattaa ggttcagccg
     1381 gaagaattga tcggacagta aagagaaatc atgatgatga atgaaataac aaagagcctg
     1441 atgtcagtcc gcaatctgat aatcaccgtt gtcgcggtgt cgttattggt tctgggcaat
     1501 ggcgattcca cactcgcaag cggtcagcaa ggccgcttga ctccaaaacc cgaacagcaa
     1561 accgtccaga gtgtagttgc gaagcaagct gctttggtta gcgagttcga ggtaaacggt
     1621 ctcaaagttc ttttaaaacg acgagaaggc agtttgactg tcgccgcagg attgtttctc
     1681 cggggtgggg ccgcaaacac gaacgctgga aacgcaggta tcgaacagtt aatgctcaat
     1741 gcttcaacgg aagcgtcaac gggtttccct cgcgaaaaga tgcggagtga actttcacgc
     1801 atgggcacca ctatcggtac tggttcgaac tacgattact cagttttggc actcgcctgc
     1861 accaggttgc acttcgatcg atcgtggcag attttttctg atgtgatcat gagaccttca
     1921 ttcacaactg aagatgtagc tctcgatcgc gatcgcatgg tggtgtcgct tagtgatgat
     1981 attgatagtc cggatgttta cctgcagcgt ttacaagaca atgtcgcgta tgcgggtcat
     2041 ccatacctta atagcaatgg gacaccagca gttatagcca agctgtcacc cgacgatctg
     2101 cgtcgctacc acgacaagat gatggaaact tctcgactct tgcttgtgat tgttggtgat
     2161 ttaaatgtag gggatattcg tgatctggtg gcaagcactg tcggaaaatt accgcgtgga
     2221 acttacaagc cggatccgct tccttcgcta agctttgaca ggtcatcggt gagcgtaact
     2281 ccccgcgatc tgccgactaa ttacgtccag ggattattca ctgctccttc gttgacctca
     2341 cctgatatct atccaatgcg cgtcgcaagt tcgatgctgc gcgacagagt ctttgaagag
     2401 gttcgtgtca aacgaaactt gtcgtatgcg ccggacgcgt ttttgcgaac ccaggctgca
     2461 aatgtgggtg gcttatatgt aaccgcggtg gatgcgaatc aatcgattag attgatgctc
     2521 aacgagattg cgaggcttca gcacgagccg gtggacaacg atgacataca atccgtcgtt
     2581 gcgcagtacc tgacgactta ttaccttggt caggaaacga atgctgcaca ggctgccgag
     2641 ttggcgcagt atgagttaat tggtggcggc tggcgcaact cggtcgattt tctcgagaag
     2701 cttacggcag tgacaccggc agaggttcag cgggtagcac agaagtacat gaagaacatt
     2761 cgttttgtgg ttttggggaa tccgcgcagc gtggattcgt cgatcttcac aggcgccgct
     2821 gccgctgcag gggtgggaag atagggccag aggccagggt tgaaggttgg tttaatcatt
     2881 ggagaattat gaattgatat ttgagaattt ctatttgaaa ccgcagcgcg ccgcgtaaaa
     2941 ccaattaata attctcaaat gtcaattcat aattctcaaa tgattaaacc aaccctcaac
     3001 ctgacccgct tttacatggt ctcccttaag tatcgctcgt acagcttttg atcgagtcgc
     3061 tgaagtactt tggcttcagt tgcggccatg ctcggat
//
LOCUS       DASSFP010000240         4478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_506500, whole genome shotgun sequence.
ACCESSION   DASSFP010000240 DASSFP010000000
VERSION     DASSFP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506500"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFH91_05235"
     CDS             <1..732
                     /locus_tag="VFH91_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6862424.1"
                     /translation="GKGRCPIVDTWWQTETGAIMITPLPGATPTKPGSGTLPFPGIDA
                     DVMTRDGKPVPNNQGGYLVIKRPWPSMLRTIWGDDERYQEQYWSQIKGIYFAGDGARR
                     DRDGYFWIMGRIDDVINVSGHRLGTAEVESALVSHSAVAEAAVVARPDDLKGSAIVAF
                     VTLEGSHTASDALSQELRAHVVKEIGALARPDDLRFTDALPKTRSGKIMRRLLREIAT
                     SGSVAGDVTTLEDFSVLEKLRTDEE"
     gene            883..1305
                     /locus_tag="VFH91_05240"
     CDS             883..1305
                     /locus_tag="VFH91_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862425.1"
                     /translation="MPRYLETENRSGPRYLASLEIKAEWDEPSGNHVIAEGTTENVGP
                     EGTLIHLPRQLPDVGSRVRLEVLGEGGERLQVVAEVLRLERNPGHPLAALQLLGETDE
                     WKGLIWEPAAPRVTPPPPPETEGDEEDADESLDDELAH"
     gene            1317..1685
                     /locus_tag="VFH91_05245"
     CDS             1317..1685
                     /locus_tag="VFH91_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6862426.1"
                     /translation="MTSPGEEDRRPQNDRRRYPRYYPFSRIMLAIEDESLNESIGIGE
                     PADISLGGVRVRNLPSCPHVKVGDRLGFLLLDSADALSLRGEVVHHATEDTFGVQFYE
                     LSINDRNAVGDLIERLHTRL"
     gene            1704..3002
                     /locus_tag="VFH91_05250"
     CDS             1704..3002
                     /locus_tag="VFH91_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6862427.1"
                     /translation="MTESENSTKHATKDAAVTAAKAAVEAYGPPTRVILRVIFIIITV
                     LVVLWMIAKLTGVILLLVLSIFFAYLVSPLVEFIGRPRKIGQRTVEMPRSLAIACAYL
                     ILLVAVFLFFSLVLPTIGNQFPEFAEQAKSYWRALGEKAQQLNEYFRLRRMPDPVVEA
                     INNAVAKVVETASSKISEFVSASVGYLAFVPWLILIPLLAFFLLKDVEGFRRSALQML
                     PRGRWRWRGDEFFQDINTTLAAYARAQLTASLFIGIVCSIGFAILGLPAWLVMGLSAG
                     VFEFVPLIGPLTVAILAALLATLHSGVFSAFLVLVFLGVLRIVQDYFIYPRLIGHGIH
                     LHPLAVIFAILCGAELAGVAGILLAIPVVAILTVSYKHWLEHRGSEGLADLLETQPAA
                     TPVKFIHQHDKHPDYDTTPEEMTHARPDLTTGELKMPVKE"
     gene            complement(3012..4118)
                     /gene="carA"
                     /locus_tag="VFH91_05255"
     CDS             complement(3012..4118)
                     /gene="carA"
                     /locus_tag="VFH91_05255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET6862428.1"
                     /translation="MTKQAPAVLALEDGRTFRGRSWSTNGEVCGEIVFNTSMTGYQEV
                     LTDPSYAGQIVCMTYPLIGNYGVNQEDSESDRPWVEGFVVREVSRITSNWRAEESLAD
                     YLQRWNIVAIEGIDTRALVRHIRDKGAMRACLSSVDLNEDSLIAKARQSPPMENRELA
                     SKVTTSARYDVPAEGVERFHVVCFDFGVKRNSLRELAQRGVRTTVVPSSTPAAEVLAM
                     KPDGVFLSNGPGDPASMNSEVEEIKQLVSATVPIFGICFGHQLLGRAFGAKTFKLAFG
                     HRGGNQPVKEVAGKGVEITAHNHGFAVEADSLPSDVEVTHLNLNDRCVEGMRHLNLPI
                     ISVQYHPEAAPGPHDAEHHFTRFIKLMEANKTTV"
     gene            complement(4182..>4478)
                     /locus_tag="VFH91_05260"
     CDS             complement(4182..>4478)
                     /locus_tag="VFH91_05260"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862429.1"
                     /translation="RDPAHSQYEVWREVAATYISANQFDDARNALEQFLEHRPSDPEG
                     LYLMGRAHAGLGHNREATSLMHACIEAVKTAPAYKYRSSKRWLNEAEQFIKGGQ"
BASE COUNT         1048 a         1149 c         1143 g         1138 t
ORIGIN      
        1 ggtaaggggc gctgcccgat agttgataca tggtggcaga ctgaaaccgg cgccatcatg
       61 atcacgccgc ttcccggtgc aacaccgaca aaaccgggct cgggaacttt acccttccca
      121 ggcatagatg cagatgtaat gactcgcgac ggcaagccag tccccaacaa ccagggcggc
      181 tatcttgtta tcaaaagacc gtggccgtcc atgttgagaa caatctgggg agatgacgaa
      241 cgataccagg agcaatactg gtcacagatt aagggtatat actttgcagg tgatggcgca
      301 cgccgcgatc gtgacggtta cttctggatc atgggtcgta ttgacgatgt gataaatgtc
      361 agtggccacc gattaggaac agccgaggtt gagagtgcgc tcgtgtcaca ctctgccgtt
      421 gccgaggctg cggtggttgc tcggcccgat gatttaaaag gttctgctat cgttgctttt
      481 gtgacgcttg aaggttcaca tacagcttcg gatgcgcttt ctcaagagtt gcgcgcccat
      541 gttgttaagg aaattggtgc gttggcccgc ccggacgatc ttagattcac tgatgcgttg
      601 ccgaagactc gctccggaaa aatcatgcgt cggctgttac gcgagattgc gaccagcggc
      661 tcagtggccg gagacgtcac aacccttgaa gacttttcag tgctagagaa gctgaggacg
      721 gatgaggaat aatagcctaa ttgatcattg gtcattgatc gtttcccatt tatcatttct
      781 tactttcttg cgctgcccgg agctaatgca aaatgacaat tggtagatgt taaatgcttt
      841 gctctgttcc caaggcaaag ctcttcttgg aggattgact gaatgccccg ctatctagaa
      901 accgagaatc gttcgggtcc acgttacctc gctagcctcg agataaaggc cgaatgggat
      961 gaaccaagcg gtaaccatgt gatcgccgaa ggcacgactg aaaatgttgg gccggaaggc
     1021 actctgatac atttgcctcg ccaattgcct gatgttggca gtcgagttcg actggaagta
     1081 ctcggagaag gtggtgaacg ccttcaagtc gtcgctgaag tactccgact cgaacgtaac
     1141 cctggccacc cactcgcagc gcttcagctt ttaggtgaga ccgacgaatg gaaaggacta
     1201 atctgggaac ccgcagcacc cagagttacg ccaccacctc ccccagaaac cgagggcgat
     1261 gaggaggacg ctgacgaatc attagacgat gagttagctc attgaggtgc tgacagatga
     1321 ctagtcctgg cgaagaagat agacgccctc aaaatgatcg tagacgatat ccccgctatt
     1381 accccttttc ccggatcatg ctcgctatcg aggatgaaag cctgaacgaa tcaattggca
     1441 ttggtgaacc ggccgatata agtctcggtg gagttcgggt cagaaatctg ccgtcttgtc
     1501 cacacgtgaa agttggcgac cggttgggct ttttgttact tgattccgct gacgcactct
     1561 ctttgcgagg cgaagtggtc catcacgcaa ctgaggatac cttcggtgtt cagttttacg
     1621 aactttcaat caacgatcga aacgccgtcg gcgatcttat tgaacggttg cacactcgac
     1681 tataaaactt taactgtgcg ctgatgaccg agtctgagaa ctccaccaag cacgctacga
     1741 aagatgctgc tgttactgcg gcgaaagccg cagtagaagc gtacgggcca cccacgcggg
     1801 ttattctgcg tgtgattttt atcatcatta ccgtgctggt agttttatgg atgattgcca
     1861 agctaacagg cgtcattctg ctactggtgc tctcaatctt ttttgcatat ctcgtgtcac
     1921 cgctcgttga gttcatcggg cggccaagaa agatcggcca gcggacagtt gaaatgccgc
     1981 gatcgctggc aattgcttgt gcctatctaa ttttactcgt ggcagtgttt ttgttctttt
     2041 cactcgtcct gccaactatc ggcaatcaat ttcctgaatt tgctgaacag gcgaaatcct
     2101 actggcgggc tctcggagag aaggcgcagc agttgaacga atactttcgc ttgcgccgga
     2161 tgcctgaccc agttgtcgaa gcaataaaca atgcggtcgc caaagttgtg gaaacggcaa
     2221 gttcaaagat aagcgagttc gtcagtgcgt cggtgggtta tctagcgttc gtaccttggt
     2281 tgatcctgat acctctcctt gctttctttt tactcaagga cgtcgagggc tttcgtcgtt
     2341 cagcacttca gatgttgccg agaggtcgct ggcgctggcg cggcgatgaa tttttccagg
     2401 acatcaacac taccctggcc gcatatgcac gtgcgcagtt aaccgcttcg ttgtttatcg
     2461 gaatagtttg ctcaatcggt tttgcgattc tcggcttacc ggcctggctg gtcatgggtc
     2521 taagtgccgg agtgttcgaa tttgtcccgc tgattggtcc attgacggtt gcaatattgg
     2581 ccgcgttgct cgcaacactg cactccgggg ttttttctgc ctttcttgta ctggtatttc
     2641 tcggtgtact gcgaatcgta caagattact tcatctatcc aaggcttatt ggccatggca
     2701 tacacttgca tccgctggct gtaatcttcg ccatcttgtg cggagccgaa ctcgcaggtg
     2761 tagccggaat cctgttggcg attccagttg tcgcaattct cactgttagt tacaaacact
     2821 ggttagaaca cagaggaagt gagggactgg cggatctact tgagactcaa cccgcagcca
     2881 caccagttaa gtttattcat cagcacgaca aacatcccga ttacgacacc acccctgaag
     2941 agatgacgca cgctcgaccg gacctcacaa ccggagaact caagatgccg gttaaggaat
     3001 gatcaaatta tctaaacggt agttttatta gcttccatga gtttgatgaa gcgtgtgaag
     3061 tggtgctccg catcatgtgg accaggagcg gcctctgggt ggtattgaac tgagataatc
     3121 ggtaaattca gatgacgcat cccttcgacg caacgatcgt tgagatttag gtgagtaacc
     3181 tcaacgtccg aaggcaaact gtcagcctcg actgcaaacc cgtgattgtg agcagttatc
     3241 tctacgcctt ttccagccac ttccttgaca ggttggtttc ctccgcgatg tccaaacgcc
     3301 agcttaaagg ttttggctcc aaaggcacga cccagaagtt gatgaccaaa acagattcca
     3361 aagataggca ctgttgcgct aaccagctgt ttaatttcct ctacttcaga gttcatcgaa
     3421 gcgggatcgc ctggtccgtt ggagagaaaa accccatcgg gcttcatagc gagcacttcc
     3481 gccgcaggtg ttgacgaagg tactacagtg gtgcgcacac cgcgttgggc caactctcga
     3541 agcgaatttc gtttgacgcc aaaatcaaag cacaccacat ggaaacgttc gacaccttcg
     3601 gctggcacgt cataacgcgc gctggtcgtc accttgctgg ctaactcacg attttccatg
     3661 ggcggcgact gcctggcctt ggcgatcaaa ctgtcttcgt tcaaatcaac cgaagataaa
     3721 caagcacgca ttgccccttt atcacgaatg tgccgtacga gcgctcgcgt atcaattcct
     3781 tcaatcgcga cgatgttcca acgctgtaga tagtcggcca aactctcttc ggctcgccag
     3841 ttggatgtaa tgcggcttac ttctcgcacg acaaaacctt caacccaggg gcgatccgat
     3901 tcgctgtctt cctgattgac accgtaatta ccaattaacg gatacgtcat acacactatc
     3961 tggccggcgt acgaaggatc agtcagcact tcctggtaac cggtcatcga tgtgttgaag
     4021 acgatttcac cacaaacctc accgttggtg gaccaggagc gtcctctgaa cgttcgacca
     4081 tcttcgagag ctaatactgc aggtgcttgc ttagtcattt ttaagttttg gatgtgcgac
     4141 tcgactcaac cgcactggtg ccaccaggga ttccagaaat tctactgtcc gcctttaata
     4201 aactgttcag cttcatttag ccagcgttta ctgcttcgat acttataggc aggagctgtc
     4261 ttcactgcct cgatacaggc gtgcatcaaa cttgtagctt cgcggttgtg gcccaatcca
     4321 gcgtgagctc gtcccatcag atatagacct tccggatctg aaggtcgatg ttccaggaat
     4381 tgctcgagag cattacgagc gtcatcaaac tggtttgctg agatataagt tgccgccact
     4441 tcgcgccaaa cttcgtactg cgaatgggcc ggatctcg
//
LOCUS       DASSFP010000241         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2005276, whole genome shotgun sequence.
ACCESSION   DASSFP010000241 DASSFP010000000
VERSION     DASSFP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005276"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /locus_tag="VFH91_05265"
     CDS             complement(<1..1940)
                     /locus_tag="VFH91_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET6862430.1"
                     /translation="MEEVFGPDGLIAQAHPEYEHRPGQIEMARAVMRAFEENRHLIVE
                     AGTGTGKTLAYLVPAVAAALGNRGRVIVSTGTKNLQEQLMEKDIPFLQSILPKPFSAT
                     YMKGRNNYLCLNRLARAQATPVLEGLEEVDYFEDVCHWARESEIGDRAELANLPESLS
                     FWRHIDARSESCLGQNCPDYEACFITRMRDRAQQADIVVVNHHLFFADLALRNGNYGS
                     VLPDYTAVILDEAHLIEDVASEYFGSQVSNYQIDDVIRDIERLEIEEAEIDREVTKSI
                     ARLATFSENFWMGFKETREEGRYPLVPGTFAKRTSSGQFESTRLGELYLFLEGSITRV
                     ETTLDTIRDKSPDVENMIRRLRQIKFDLQFIVTGSDKKFVYWLERRNRGVFLRASPID
                     VAGLLQDKLFDEVPTVVLTSATLSSGGNFAFIRDRLGLDSADDLIAESTFDYESQAIL
                     YLPPKMPEPRSREWSEAAAAEVINILNATQGRAFVLSTSLAGMQSLFESVWAEIDYPC
                     LVQGSASKSQLLKRFRETPHAVLFATSSFWQGVDVRGEQLSCVIIDKLPFAVPTDPIV
                     AARQRYIEDSGGSSFHEYSVPQAIISLKQGLGRLIRSTSDRGVLAVLDPRLRTKAYGQ
                     TFLRSLPPCRTTSRIDDLAAMF"
     gene            complement(1947..3239)
                     /gene="glyA"
                     /locus_tag="VFH91_05270"
     CDS             complement(1947..3239)
                     /gene="glyA"
                     /locus_tag="VFH91_05270"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET6862431.1"
                     /translation="MNDIRNAPLAEVDPIVAEAIDHEVARQANGLELIASENFVSEAV
                     LEAAGSVFTNKYAEGYPGKRYYGGCEWTDVVEQTAIDRAKQLFGSDHANVQPHSGAQA
                     NMTVYLAAIQYGDQILGMNLSHGGHLTHGHPMNFSGLSYKVADYGVSKDTEQIDYDEL
                     QRIAEEHKPKLLVCGASAYPRTINFERIGEIARSVGARMFADIAHIAGPVAAGLHPSP
                     VPHADYVTTTTHKTLRGPRGGLILCKEEHAKEIDRKLFPGVQGGPLVHIIAAKAVAFG
                     EALREDFKDYQSQVLKNAKALASELQEQGLRLVSGGTDNHLMLVDVWMDGKGTTGKDA
                     EKALEAALITVNKNTIPFDQNKPFVASGLRIGTPAVTTRGMKEAEMQEIGRLIAEVIH
                     APESEEVRKKVRQGVSDLTARFPLYAKRLKASSIGLDR"
     gene            complement(3249..3689)
                     /gene="rpiB"
                     /locus_tag="VFH91_05275"
     CDS             complement(3249..3689)
                     /gene="rpiB"
                     /locus_tag="VFH91_05275"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET6862432.1"
                     /translation="MKIALASDHAGFAQKERLKSLLTEIGVDFEDLGTCSEESVDYPD
                     YARKVAEQVAEGRVAQGLLVCGSGIGMAISANKVNGVRAALAWSEETARLARQHNDAN
                     VLAIGARTTQPGEIPKIVRAWFESDFEGGRHRMRVEKINAIERR"
     gene            3690..>4361
                     /locus_tag="VFH91_05280"
     CDS             3690..>4361
                     /locus_tag="VFH91_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HET6862433.1"
                     /translation="MVDSLREELLRRSRMVHQPNLARKPVPKKDRPSEFALGVQLSEN
                     ARCSSVRDSGYIYSNKSMRWSQFFIPTLRENPADADVISHKLLLRAGLIRQLSAGIYS
                     FLPLGQRVALKVMQILREEMNAIGGQEFYLPALHPAEIWQESGRWAAIGDEMFRLKDR
                     KGADMCLGMTHEEVFTTIARNEINSYKQLPQVWYQIQIKFRDEARPKSGLMRLRTFIM
                     KDAYSF"
BASE COUNT         1033 a         1149 c         1079 g         1100 t
ORIGIN      
        1 ataaacatcg cggctagatc atcaatgcgt gacgtggtgc ggcaaggtgg caagctgcga
       61 aggaaagttt gtccataggc tttagtgcgg agccgcggat ccagaaccgc gagcactcca
      121 cggtcgcttg tacttcgaat caagcgtccc aggccttgtt tcaaagagat tatagcctgc
      181 gggacactgt actcgtgaaa gctcgaacct ccactatctt caatgtaacg ctgtctcgcg
      241 gcaacaattg gatcagttgg cacagcgaac ggaagtttgt cgatgatcac gcatgacaat
      301 tgctctcctc gcacgtcaac accttgccaa aaactcgaag tcgcaaaaag aacggcgtgc
      361 ggagtttctc tgaaccgctt caacaattga ctcttcgaag cactgccttg aaccaggcag
      421 gggtaatcaa tttcagccca cacactctcg aacagcgact gcattcccgc gagactggtt
      481 gaaagtacaa atgcgcggcc ttgagtcgca ttcagaatgt tgatgacctc ggcggctgcg
      541 gcttcagacc attctcttga tcggggctcg ggcatctttg gaggcagata gagaatcgcc
      601 tgactttcgt aatcaaatgt cgactcggca attaagtcat ccgccgagtc gagtccaagt
      661 cgatcgcgga tgaatgcgaa attgcctcca ctggaaagag tggctgaggt cagaactact
      721 gttggtactt catcgaagag cttgtcctgt aaaagaccgg caacatctat cggcgaagca
      781 cgcaagaata cgcctctgtt cctccgttca agccagtaca caaatttctt gtcagatccg
      841 gtaacaatga attggagatc gaacttgatc tgtcttagac gtctgatcat attctcgacg
      901 tcgggactct tgtcgcggat ggtgtcgagc gtagtttcga cgcgagtgat agagccctct
      961 aaaaacaaat acaactcacc aagccgcgtg gactcgaact ggcctgagct ggttcgtttt
     1021 gcgaacgtgc cgggcacgag cggatagcga ccttcttcac gggtttcttt aaagcccatc
     1081 cagaaattct cggaaaaagt agcaagacgc gcgatactct ttgtcacttc gcgatcaatt
     1141 tccgcttctt cgatttcgag tcgttcgatg tcacggatga cgtcatctat ctgataattc
     1201 gaaacctgcg agccgaagta ttcagaagct acgtcttcga tcaggtgtgc ctcgtcaaga
     1261 atgactgcag tgtaatcagg caagactgaa ccatagttgc cgttgcgcaa cgccaggtct
     1321 gcaaaaaaca agtgatggtt cacaaccaca atatcggctt gttgagcgcg atcacgcatg
     1381 cgggtgataa agcacgcttc ataatcggga cagttctggc cgaggcaaga ttctgaacgc
     1441 gcgtcgatat gtcgccaaaa ggatagggac tctggaagat ttgcgagttc agcacggtcg
     1501 ccaatttcgc tttcccgtgc ccagtgacag acgtcttcga agtaatcaac ctcttccagc
     1561 ccttcaagga ctggagtggc ttgtgctcgg gccaggcgat tgagacagag atagttgtta
     1621 cgacctttca tgtatgtggc tgagaagggt ttgggcaaaa tgctctggag gaacggaata
     1681 tccttttcca ttagctgttc ctgcagattc ttagtgccgg tggagacaat cactcgacca
     1741 cggttgccaa gggcggcggc gacagcgggg acaaggtagg ctaacgtttt gccggttccg
     1801 gtcccagctt cgacgatgag gtgtcgattc tcttcgaagg cgcgcataac ggcgcgggcc
     1861 atttcgattt ggccgggacg gtgttcgtat tcgggatgtg cctgagctat caagccatca
     1921 ggcccaaaaa cttcttccat aatccactaa cgatcaagtc cgattgatga cgctttcaat
     1981 ctctttgcgt acagcgggaa tcgagcagtg agatcagaga cgccctgccg caccttcttc
     2041 cgaacttctt cagactcggg agcgtgaatc acctccgcta tcagacgccc aatttcttgc
     2101 atctccgctt ccttcatgcc gcgagttgtt accgcgggcg taccaattcg taggcccgat
     2161 gcgacgaatg gtttgttctg atcaaaaggt atcgtgttct tgttcacggt tatcagcgcc
     2221 gcttcgagag ccttctcggc atccttacct gtagtgcctt ttccatccat ccaaacatca
     2281 accaacatta agtgattgtc tgttccgcct gaaacgagac gcaaaccttg ctcctgtaat
     2341 tcgcttgcaa gggctttggc atttttcagg acctggcttt ggtagtcttt gaaatcttcg
     2401 cgaagcgctt cgccgaatgc gacggctttt gcagcaatga tatggaccaa aggcccgcct
     2461 tgaactcctg gaaacaactt ccgatctatc tccttcgcat gctcttcttt acacagaatt
     2521 aagccaccac gcggtccacg aagcgtctta tgcgttgtcg ttgtcacata gtccgcatga
     2581 ggtaccggcg aaggatggag tccggctgcg actggaccag caatatgcgc gatgtcggca
     2641 aacattctcg ctccaacact ccgtgcaatc tccccaattc gttcaaaatt tatggtacga
     2701 ggataggctg aagctccgca cacaagcagc tttggtttgt gctcttctgc gatcctctgc
     2761 aactcgtcgt agtcgatctg ttcagtatct tttgaaacac cataatcagc gactttgtaa
     2821 ctcaacccgg aaaagttcat cggatggccg tgcgtgagat gcccaccgtg tgacaggttc
     2881 atcccgagaa tctgatcacc gtattgaata gccgccaggt aaacagtcat gtttgcctgc
     2941 gcgccactat gcggttgaac gttagcgtga tctgaaccga agagctgctt tgcgcggtca
     3001 atcgcagtct gctcgacaac gtccgtccat tcgcagcctc cgtagtaacg ctttcccgga
     3061 tacccttcgg cgtatttgtt cgtaaaaact gaacccgcgg cctcgagcac tgcttcgctg
     3121 acaaagtttt ccgaggctat aagttcgagg ccattcgcct ggcgtgctac ctcatggtcg
     3181 atagcttcag ccacgatcgg atcaacctct gctaatggcg cgttgcggat atcattcatg
     3241 aatattcttt atcgcctttc gatggcattg attttttcaa ctcgcattcg atgacggcca
     3301 ccttcaaaat cggattcaaa ccaggcccgc acaatttttg gaatctcgcc gggttgagtg
     3361 gtgcgggctc cgatcgcgag aacgtttgcg tcgttgtgtt gcctggccag cctcgcggtt
     3421 tcttcagacc aagctaaagc cgcgcgaacc ccatttactt tatttgcact gatagccatt
     3481 ccaattcccg aaccacacac cagcagtccc tgagcaactc gaccttcagc tacttgctcg
     3541 gcaacctttc gcgcataatc cggataatct acggactctt ctgagcaagt gcctagatct
     3601 tcgaaatcga cacctatctc ggtaagcaaa gacttgagcc gttctttttg ggcaaagccc
     3661 gcgtgatcac tggccagggc aattttcata tggtcgatag ccttcgtgaa gagttgttaa
     3721 gaagaagccg catggtacat cagcccaatc tcgctcgcaa accggtcccg aaaaaggatc
     3781 gaccttctga gtttgcgttg ggcgtacagc taagcgagaa tgctcgatgt tcttcagtcc
     3841 gcgattcagg ttacatatac tccaacaaaa gtatgcgatg gtcacaattt ttcattccaa
     3901 ctctacgcga gaaccccgct gatgctgatg ttatctcgca caaactgctt ttgagagcag
     3961 gtcttatccg ccagttatcg gcaggcattt actcttttct accgctgggc caacgtgtgg
     4021 ctttgaaggt tatgcagatt ttgcgcgagg aaatgaatgc tattggtgga caggaatttt
     4081 atctacccgc gctccacccg gccgaaatct ggcaagaatc gggtcgttgg gcggcaatcg
     4141 gtgatgagat gttcaggctt aaggatcgta agggcgcaga catgtgtctt ggtatgacgc
     4201 acgaagaagt ctttacaacc atcgctcgga acgaaatcaa ttcatacaag caactgccac
     4261 aggtttggta ccaaattcag atcaagtttc gagacgaagc tcggcctaaa tccggcttga
     4321 tgcgtctgcg aaccttcatc atgaaggacg cttattcctt t
//
LOCUS       DASSFP010000242         6638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1552626, whole genome shotgun sequence.
ACCESSION   DASSFP010000242 DASSFP010000000
VERSION     DASSFP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552626"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..585
                     /locus_tag="VFH91_05285"
     CDS             310..585
                     /locus_tag="VFH91_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862434.1"
                     /translation="MSLTKEDAVSKARKDLAKRLNVEENSIKEKSIDEVDFPDMALGA
                     GTEDEMSGQMLTRGWRIRLDSTGKTFEYRADKNQLRLFNFQGKNYRI"
     gene            645..2048
                     /locus_tag="VFH91_05290"
     CDS             645..2048
                     /locus_tag="VFH91_05290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET6862435.1"
                     /translation="MNRNNFLPHIALALVLILVAAFAGQLRRWQKQWKSQVRIIPAEK
                     KQPTTIDEIALTDAEHLGRPEILVRFKPGVSAETIDRITSQLHDRVEDRIENAPGLDA
                     IDDLDDENAAELVAQYSKMPEVEYAEENYEISLDVVEGPLVPVFPHDPQFTDQWALSN
                     QGQRGGKPGADISAPLAWATTTGSDKVVVAVLDSGVDYRHDDLANNMWVRPAEMAPYH
                     DDDLGTIDDVNGFNAIDKGSDPMDDNGHGTHCAGIIGAEGSNDIGIAGVDWNVRIMPL
                     KFMNAGGFGTTKDAIEAINYVIERKKAGVNVRVISASWGSTQKSRALEDIIRKAYEEG
                     ILFVAAAGNASTNNDRNPHYPSSYNVPNVISVAALDRHDQLASFSNYGPKSVVIAAPG
                     VDILSTWLGNEYEEKSGTSMATPVVAGVAALVLAHEPGLSVDELKKKLLTSVDKLDSL
                     AGKVSSGGRINAAKAVE"
     gene            complement(2236..4062)
                     /locus_tag="VFH91_05295"
     CDS             complement(2236..4062)
                     /locus_tag="VFH91_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862436.1"
                     /translation="MSQFLLSVLLLLSLSGVLYAQTPTSSPRPAQQQTKPDRQPIAFD
                     LNEYGVQFEADPRLIVVMAALEVAGLDTVPTGREPSAFRSKVRKDLANLDPDLRQRLR
                     NFYERNRLAAPATAADQTSRYVSLALALGPPPGLEAPIRSEDLPAGLLEVLDFASLVQ
                     EFYRRSGIDEQLVSYTRAYQAEGDRLRAPTAEMVRSILSYLHTRPITVTNERVEVKNT
                     DNKKSDSKVKRYTLHQKDRRFRVMPDLLAAPGAINFRVIGDDYYAIVPEGTEPGSSEL
                     IRAYLQYVIDALVLRFNKEIADRREQVKLLLDERQKAGAEVSPDVFLSVTRSLVAAAD
                     ARYNEQRRLEILAIDVRSRLQAAKDDATRAAITRTTQAQVAEIKDETVARLADEYEKG
                     AVLSFFFADQLKGVETSGFDIANFFPDMIASFDPAREAKRPAEYAEARQRAVAARAAR
                     AAARKAGADLDNSAANVRDAGLVKELTTVEQTLRNKDYTDAETKLKSMLASYPREPRI
                     FFALGQTASLAAMDATDETIRDERLNRALGHYRLAVQSASPETDKAVLSRAHESMGRI
                     NAFLDNKVEAAKEFDEAIKLGEVTGGAYKEALEGKKKLAQPE"
     gene            complement(4155..4622)
                     /locus_tag="VFH91_05300"
     CDS             complement(4155..4622)
                     /locus_tag="VFH91_05300"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET6862437.1"
                     /translation="MFRSTIRRLLAIDDPPERTALAFSIGVFIAFSPFLGLHTILATL
                     IAFIFRFNKVAIYTGTFINNPFLTLVPIIVASYGVGAFILGRPMHIPQEGVDFLKDPE
                     IFSGSYYRRLFSQSRYIVEPFAVGGMVLSVVCSVIAYPLTLRALRLYGIRRHG"
     gene            complement(4685..5032)
                     /locus_tag="VFH91_05305"
     CDS             complement(4685..5032)
                     /locus_tag="VFH91_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6862438.1"
                     /translation="MAELDVKERQAGDVTILDLSGAVRIGEGSIALRDAIRSLADEGK
                     KKVLLNLRGVNYVDSSGIGELIANYTTISRQGGQLKLLNLTDRIQNLLVITKLLTVFD
                     AYDNEAEALKSFE"
     gene            complement(5068..5415)
                     /locus_tag="VFH91_05310"
     CDS             complement(5068..5415)
                     /locus_tag="VFH91_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6862439.1"
                     /translation="MAELNIEERQAGDVTVLDMSGKITIGEGSVALRTAIRRLLEEGK
                     KRILLNLAGVGYIDSSGIGELVSSYTAIGKENGQLKLLNLTQKLQDLLTITKLLTVFD
                     VYESEAEALNSFK"
     gene            complement(5444..5857)
                     /locus_tag="VFH91_05315"
     CDS             complement(5444..5857)
                     /locus_tag="VFH91_05315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862440.1"
                     /translation="MTVEKTELSLPSRIETVATAAAAVAEFVSRSGVSDDAAFGIDMA
                     VREAVTNAVVHGNRQDENKVVDITLQSSPDAVEISVHDQGPGFNPSDVPDPTAAENIL
                     KTSGRGIFFMRSFMDEVDWLIRPEGGTTVRMLKRR"
     gene            complement(6101..6484)
                     /locus_tag="VFH91_05320"
     CDS             complement(6101..6484)
                     /locus_tag="VFH91_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6862441.1"
                     /translation="MASIISPVILVAEDSHDTRRMLKRAFELKGFRVLEAVNGREALA
                     LTKQYRPSLLIIDLNMPELDGFETIRRVRKLKEGEDLPIVAITAFDVYGMEQAVLDQG
                     CNEYLTKPFDLHELDRRLKGLGFVV"
BASE COUNT         1685 a         1703 c         1635 g         1615 t
ORIGIN      
        1 gggtcaggat tcaggggtcg gagtatttga taattatgaa ttgagatttg agaattttta
       61 tttgatctag ttgcgcccgg cagtctggca aacggcacag atcttcaaac gcgacccgca
      121 tagcaaataa caattctcaa atctcaattc ataattatca aatactccga cccctgaatc
      181 ctggccccta acccctattc ctgataccca ttccttgacc ggaggggccg acagtttcaa
      241 aatgggttcg gaacaaagca ttcaattggc acgttttgag actagtatga cttccaaagg
      301 gattcactga tgtcgttaac caaagaggac gccgtttcta aagctcggaa agacctggca
      361 aaacgtctaa acgttgagga aaactcaatc aaggagaagt cgattgacga ggtcgacttc
      421 ccggacatgg cgttgggcgc tggaacagaa gatgaaatga gtggtcaaat gttaacgcgc
      481 ggatggcgca ttcgtcttga ctcgacaggc aaaacttttg aatatcgagc ggataagaat
      541 cagctcagac tctttaattt ccagggtaag aactacagga tttgagcgtt ttgacgattg
      601 ttactcgaaa gtcggaacgc atttgctaac tgaggtacca ggtaatgaac cgcaataatt
      661 ttctgccaca catcgcactc gcattagttc tcattctagt cgccgcattc gccggtcaac
      721 tgcgccgctg gcagaaacag tggaagtctc aagtgcggat tatccctgct gaaaagaaac
      781 agccgaccac aatcgacgag attgctctca ccgatgccga acatttggga cgacccgaaa
      841 tcctggtgag attcaagcca ggagtcagcg cagaaacaat cgatagaatt acttcccaac
      901 ttcatgatcg cgtagaggat cgaattgaaa acgccccggg ccttgacgct attgatgatc
      961 tcgacgacga gaacgctgct gaactcgtcg cgcaatatag caagatgcct gaggtcgaat
     1021 atgcggaaga gaactatgaa atcagtctcg acgtcgttga gggaccactc gttccggtgt
     1081 tcccgcatga tccacagttt accgatcaat gggccttatc aaaccaggga caacgcggtg
     1141 gaaaaccggg tgctgatata agcgcaccgt tagcatgggc cactaccact ggcagcgata
     1201 aagtggtcgt tgcggttctc gacagtggag tcgattaccg ccacgatgat cttgctaaca
     1261 atatgtgggt ccgaccggct gagatggcgc cataccatga tgatgacctg ggcaccatcg
     1321 acgacgtcaa cggatttaac gcaatcgaca agggctcaga tcctatggac gataatgggc
     1381 acggtactca ctgtgctgga atcatcggcg ccgaaggcag taacgacatc ggaatcgcag
     1441 gcgtggactg gaacgtccga attatgccgc ttaaattcat gaacgccggc ggattcggaa
     1501 ccaccaaaga cgctatcgaa gcgatcaact acgtcatcga acgtaagaag gcgggtgtga
     1561 acgtacgcgt tatcagcgca agttggggct cgacccagaa gtcgcgcgct ctggaagaca
     1621 tcatccgcaa agcttatgaa gagggaatcc tgtttgtagc tgctgccgga aacgctagta
     1681 ccaacaacga tagaaatccg cattatcctt ccagctacaa cgtgccgaat gtaatcagtg
     1741 tagctgcact cgatcgtcac gatcagctcg catcattctc gaactatggt ccaaagagcg
     1801 tcgtgattgc cgctcccgga gtcgacatac ttagcacatg gttaggaaat gagtacgaag
     1861 aaaagtcggg cacttctatg gcaacgcccg tcgttgccgg agtcgcggct ctggtgttgg
     1921 ctcatgaacc aggcttgagc gtcgatgagt tgaagaaaaa gctgttgacg tccgtggata
     1981 agctggactc actggcgggc aaggtcagtt cgggtggccg aatcaacgca gccaaagcag
     2041 tagagtaggg tcaggggtta gaggccagaa ttcagggctc ggatttattt gataattatg
     2101 aattgagatt tgagaattgt tatttgcttt gcgggtcgcg tttgaagatc tgtgccgttt
     2161 gccagaatgc cgggcgcaac tagatcaaat aaaaattctc aaatctcaat tcataattat
     2221 caaataattc gggccctatt ccggttgggc caacttcttc tttccttcga gggcttcctt
     2281 gtaagccccg ccagtgactt ccccgagctt tatcgcctcg tcaaactctt ttgctgcctc
     2341 taccttgtta tcgagaaacg cattgattcg tcccatcgat tcatgagctc tggataaaac
     2401 tgccttgtcc gtttcaggag aagcagactg gactgcaagt cggtaatggc ccaaggctcg
     2461 attcaaacgt tcatcccgaa tcgtttcatc agttgcgtcc atcgccgcca aactcgccgt
     2521 ttgccccagg gcgaagaaga tccttggctc acgaggataa ctggccagca tcgacttcaa
     2581 cttcgtctct gcgtcggtgt agtccttgtt gcgtaacgtt tgttcaaccg tagttaattc
     2641 ttttaccaaa ccggcatctc taacgtttgc tgcagagttg tctaaatcag ctccggcttt
     2701 ccgcgccgca gcccgcgcgg cgcgagcagc tacggcccgt tgacgcgctt cagcatactc
     2761 ggctggacgc tttgcttctc gagctggatc aaacgacgcg atcatgtcag gaaagaaatt
     2821 agcgatatcg aaacctgacg tctcaacccc cttcaattga tcggcaaaga agaacgacag
     2881 cacagctcct ttctcatact catcggcaag tcgcgccact gtctcatcct tgatttccgc
     2941 tacctgagcc tgcgttgtcc tcgtaattgc cgctcgcgtc gcgtcatcct ttgcggcctg
     3001 caatcgactg cggacatcta ttgcgagtat ttcaagtctt cgctgttcgt tgtaccttgc
     3061 gtcagcagcg gccacgagcg aacgagttac actaagaaag acgtccggac taacctcagc
     3121 ccctgccttt tgtcgttcat ccagcaacaa cttaacctgc tctcgccgat cagcaatttc
     3181 cttattgaaa cgaagaacaa gcgcatcgat cacgtattga agataagctc ggatcaattc
     3241 agaagatccc ggttcggtac cttctggaac aatcgcgtaa tagtcatcgc ctataactcg
     3301 gaaattgatg gctcccggag cagcgagcaa atcgggcatc acccgaaacc ggcgatcctt
     3361 ctgatgtagc gtgtatcgct tgaccttact atcgctcttc ttgttatcag tgttctttac
     3421 ttcaacacgc tcgttagtca ctgtgatagg tcgggtatga agatagctaa ggattgatcg
     3481 aaccatctca gccgttggcg ctcttagcct gtcgccctca gcctgataag cccgcgtgta
     3541 actcaccagc tgttcgtcaa tcccggatcg tcgatagaat tcctggacca atgacgcaaa
     3601 gtccaatact tctaaaagtc cggccggcag atcttccgaa cgaattggag cctccagccc
     3661 tggaggcggt cccaaggcga gcgccagtga aacgtaacga gaggtctggt ccgccgcggt
     3721 cgccggtgct gcaagacggt tccgctcata gaagttccgc aaccgctgtc tcaaatccgg
     3781 atcgagattg gccagatcct ttctaacttt tgacctgaag gcggaaggtt ctcggcctgt
     3841 tggaacggta tcgagcccgg ccacttccag tgcggccata accacaatta atctgggatc
     3901 ggcttcaaac tggacaccat attcgtttag atcgaaggca atcggctgcc gatccggctt
     3961 ggtttgttgt tgggcaggac gcggagaact cgtcggagtt tgcgcgtaca gaactcctga
     4021 caatgaaaga agcagtaaaa cactcaataa aaactggctc atatcgatca atgttgaaat
     4081 tgcgaacagg tttagtcgaa gaaaaaatag atgcgctaag cgagtacgtc gatgccaaca
     4141 cggtgaagag acgattagcc gtgtcgtcgg atcccatata aacgcaacgc tcgcaatgtt
     4201 agcggatatg caataactga acaaaccacc gagagaacca ttccgccaac ggcaaacggc
     4261 tcgactatgt aacggctttg tgagaagaga cggcggtagt aagagccgga aaagatctcg
     4321 ggatccttca aaaagtctac tccttcctgg ggaatgtgca tcggacggcc cagaatgaag
     4381 gcgcccactc cgtatgacgc aacgataatt ggcacaagtg ttaggaatgg attgttgatg
     4441 aacgttcctg tgtagatcgc gactttattg aagcgaaaga tgaatgcgat cagcgtggca
     4501 agaatcgtat gaaggccgag gaatggggaa aatgcgataa aaacgcctat tgaaaaggca
     4561 agcgccgtcc gctcaggtgg atcatcaatc gcgagcagac ggcgaatagt gctacgaaac
     4621 atgtgtaaac aggcttactg gtctgtcagc ttcgacgcag actaaacaga cacgttttgc
     4681 ctgtctattc aaaactcttc agggcttcag cctcattatc gtaagcgtca aaaacggtaa
     4741 gcaactttgt tatgaccaat aggttttgga tccgatcagt caggttcaat agttttaact
     4801 gaccgccctg gcgactaata gtggtgtagt tggcaattaa ctcgccaatt ccactcgagt
     4861 cgacatagtt aacgccgcgt agattaagga gaaccttttt ctttccttcg tcggctagcg
     4921 atctaatggc atctcgcaaa gcgatgctac cctcaccgat acggactgca ccacttagat
     4981 ctaaaatggt gacatctccg gcttgtcgct ctttcacatc cagctcagcc atttctcact
     5041 ccgtctgcgt cgacccgagg caggttatta tttaaaactg ttgagggcct ccgcttcact
     5101 ttcataaaca tcaaagaccg taagaagttt ggtaatcgtc agcaagtctt gaagcttctg
     5161 agtgagattc aataacttca actgaccatt ctctttcccg atggcggtgt agctcgagac
     5221 caattctcca ataccgcttg aatcaatgta accgactccc gcgaggttca gcagaattct
     5281 cttcttacct tcttcgagaa gtcttctaat ggcggtacga agtgcaacac ttccctcgcc
     5341 gattgttatc tttccactca tgtcaagaac ggttacatct ccggcttgac gttcctcaat
     5401 atttaactca gccatttcta gaggtctcct ttaggtctgc gattcaccgc cgcttgagca
     5461 tccgcactgt tgtaccacct tccggcctga tcaaccaatc aacttcatcc atgaaactgc
     5521 gcatgaaaaa aattccgcgg ccggaagttt ttaagatgtt ttctgcagct gtcggatccg
     5581 ggacgtcgct gggattgaac cctgggccct ggtcatgcac gctgatttcc acagcgtcag
     5641 gcgagctttg caaggtgata tcaacgacct tattttcgtc ttgacggttg ccatggacga
     5701 ccgcgtttgt aaccgcctcg cgcacggcca tatcgatacc aaacgcagca tcatcgctaa
     5761 cccctgagcg gctcacgaat tcagcaaccg cagcggccgc ggttgcgacc gtttcaatgc
     5821 ggctgggcaa actgagttcg gtcttttcta ccgtcacagc gattaggcat ggcctctcca
     5881 aaaagccact gcgcgaggac cctgggctga tctcgaataa cgtccgaaac ttctcggaag
     5941 gtcgagggtg taacatgctg gctggccgcc tgtcaagaat tggcaccagt agatattgtc
     6001 ccagtcgccg cgtggcgacc ctcgttgggc ggcttttctg acgtgtcaga tgcgaagaat
     6061 aagggctttg gggagaaacg ccattacaga ttcctgcgct tcacaccaca aatcccaacc
     6121 ccttcagccg gcgatctaat tcatgtagat caaatggctt agtaaggtat tcgttgcagc
     6181 cttgatcgag aaccgcttgt tccattccgt aaacgtcgaa ggcggtgatg gcaacaatag
     6241 gaagatcttc gccctctttg agtttccgca cccgacgaat cgtttcgaac ccgtccaatt
     6301 cgggcatgtt caagtctatg attaacaggc ttggtctata ttgcttggtt aaagctaagg
     6361 cctcacgtcc gtttactgcc tcaaggacgc gaaaaccctt tagttcgaag gcccgcttga
     6421 gcatgcgccg ggtgtcatgc gagtcttccg ctacaagtat tacaggagaa attatggatg
     6481 ccacggctat ccttcgccaa tcaggcgcag tactgatgct gagatacgct ggaggctact
     6541 ttcagggagc tatagggtct gttagggtat gctcacatgt ctttcgtgaa attgctaaag
     6601 atatccctaa actatggcga gtgttaaata agtttgtc
//
LOCUS       DASSFP010000243         3415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_507025, whole genome shotgun sequence.
ACCESSION   DASSFP010000243 DASSFP010000000
VERSION     DASSFP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507025"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..2366
                     /locus_tag="VFH91_05325"
     CDS             537..2366
                     /locus_tag="VFH91_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862442.1"
                     /translation="MPKPELRLPVITILCLSIISLVSIFLFGGQKTAAVASGSSEKSS
                     ITVFSSARNDVSQPLRTLSTRTILTDSENISAPSPTPTPKMSPVTRESAAVEQTHHGT
                     RLSARIVESFDGLGFGFEGPQGKANLRNPSDNTLAVGPDHVVQIVNTRMAIFTKKGKR
                     FDSTGKVLMGPIVTRSLFTGFGGQCEARNNGDAVARYDQLAGRWLIVMPIFARGPVRP
                     DQPRVGTSGEPAALSPPGQTNQPGTQTKLSPPAVANPADPNRAAAATAGDGPYSMCYA
                     VSVSSDPLGAYYRYEFLRPFFPDYPRPAVWPDGYYVPTSTSDDIIQKHACVVDRNSML
                     KGLPATEQCFVIDGVNFLNNADIDGFGVPPSGAPNIMMAAGGAQLKGVLEDDKIFFWN
                     FHVDWKTPANTKLDGPFPITVAPYQYLCGGQLTDCVPQPGTDRRLDSQGDKIMARLVY
                     RKFRDHESIVAVHSVNTASGSGGVRWYEFRIGRAREPKLYQQGTYAPDNSYRWMGSPA
                     LDRYGNLGIGYSFGSGNDFPGQRFAARLSNDPLGQLTLNETVLVQGEGVQNVMRWQDY
                     TQTAIDPSDDCTIWYVGDYVKKDTPFYSSRIGAFRLPGCGRAR"
     gene            complement(2369..>3415)
                     /locus_tag="VFH91_05330"
     CDS             complement(2369..>3415)
                     /locus_tag="VFH91_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6862443.1"
                     /translation="VIVKAHHAHPGTSELVGLAVMDAVQSCDLPEGVFSLIYGSGNDV
                     GTALVKHPLIKAGGFTGSRAGGRALFDAASSRPEPIPFYAEMSSVNPVVILPGALSER
                     SDQIAAGLSGSVTLGAGQFCTNPGLIFLADSQESHSFVQKLGTLMSESPEQTMLTEGI
                     SSTYKKSVAKRSGHMAVETLVSTASAQQQEGCGARTALFLTNAKEFMANPELAEEVFG
                     PSTLVIMYSNKQELLAAIDQLEGQLTATVHANDEDMSSSSELVAALEAKVGRLVFNGF
                     PTGVEVCHSMVHGGPYPATSDGRSTSVGTRAIFRFTRQVCYQDFPNGALPDELKNENP
                     LGIWRLVDGELAKK"
BASE COUNT          822 a          920 c          853 g          820 t
ORIGIN      
        1 ccgctcgcgc aagagttcca ccatcacgct gccgatataa ccggcgccac ctgtaacgag
       61 tacagccatg aggacgactc cctaaaaata attgttagtg ggaaatgcaa ctcgacttta
      121 tcatcaggat aagtagctga ctagtcgctt gttcgattca gtttgctttc ctttgcgttc
      181 tctgcgtgat ctccgcgtac tctgcgtctt gtcggagttc cttattagag cgacacagcc
      241 aagacgcaga gaacgcggag atcacgcaga gaaacgccga gaaatgaaac tgaaccagta
      301 cctctgtgcg aggtccatcg aggagttggc ggcagtcgct gcgctcatgg ttgtgtaact
      361 caggcgcggt gtcctatatg cttcagcttg tcggtttgac tcgttcctct gaacttgagt
      421 ctgcatcatg accgacaagc tgaagcatat cggacatccc cggaatatgt tgacgaaggt
      481 ctctattaat cggtagaaga gagtcatctc gtttaaccga ctttcccgag gaaccaatgc
      541 cgaaacctga gctacgcctg cctgttataa ccatcctctg cctttccatc atttccttag
      601 tttctatttt tcttttcggc gggcagaaaa cagcagctgt cgccagtggt tcgagcgaaa
      661 aatcatccat cactgttttc tcgagcgcaa gaaatgacgt ctcgcagcct cttcgcaccc
      721 tatcaacaag aacgattctt actgacagcg aaaacatctc ggcgccttcg ccaactccta
      781 ccccgaaaat gtcacctgtc acacgagagt cagcagccgt agaacaaact catcacggta
      841 ctcgtctgtc agccaggatc gtcgagagct tcgacgggtt gggcttcggt ttcgaaggac
      901 cacagggcaa agccaatctc cgtaatccct ccgataacac tctggcggtc ggacccgatc
      961 acgtggtcca aattgttaac actcgaatgg cgatctttac gaagaaaggc aaacggtttg
     1021 attcaaccgg caaggttctg atgggcccaa ttgtaacgcg atctctgttt acagggtttg
     1081 gcggtcaatg cgaagctcga aataatggtg atgcggttgc tcgatacgac caactggcag
     1141 gtcgttggct catcgtgatg cccatctttg cacgtggtcc ggtgcgaccc gatcagccac
     1201 gtgttggcac atccggagaa ccagcggcac taagcccacc agggcaaact aatcaacccg
     1261 gtacgcaaac aaaactaagt cctccggccg tggcaaaccc cgcagatcca aatcgtgccg
     1321 cagcagcgac agcgggagat ggtccttatt caatgtgtta tgcggtaagc gtcagctccg
     1381 atcctttagg ggcgtactac cgttacgaat ttcttcgtcc gttcttccct gattaccctc
     1441 gaccagccgt ttggcccgac ggctactatg tcccaaccag cacgagtgac gacattattc
     1501 agaaacacgc ctgcgtagtt gatcgcaaca gcatgctcaa aggtttaccg gcgaccgagc
     1561 aatgttttgt gattgatggt gtcaattttt tgaacaacgc ggatatcgac gggttcggag
     1621 ttccaccctc gggcgcgcct aacatcatga tggctgcagg tggagcacag ttgaagggcg
     1681 ttctcgagga cgacaagatc ttcttctgga actttcacgt tgattggaag acgccggcta
     1741 acaccaaact tgacggccca tttcctatca cggtcgcacc ttatcaatat ctttgcggtg
     1801 gccagttgac tgactgtgtg cctcaacccg gtaccgatag aagactcgac tcacagggcg
     1861 ataagattat ggcgcgcctg gtctatcgca agtttagaga tcatgaaagt attgtggctg
     1921 tccactccgt aaacactgct tctggatctg gaggtgttcg ttggtatgag ttcagaatcg
     1981 gtagggctcg cgaaccgaag ttatatcaac aaggcactta cgctccagac aattcgtacc
     2041 gttggatggg aagcccggca ctcgaccgtt acgggaatct aggtattgga tattccttcg
     2101 ggagtggtaa tgatttcccc gggcaacggt ttgcggctcg cttatcgaat gatccactgg
     2161 gccagttgac actgaacgaa accgtgttgg ttcaaggtga aggggtgcag aatgtcatgc
     2221 gctggcaaga ctacacgcaa accgccatcg atccgagcga cgactgcaca atctggtacg
     2281 ttggcgacta cgtcaaaaag gacacgccgt tctacagcag cagaattgga gcgtttcgtt
     2341 taccgggctg cggacgagcg agatagcatc acttctttgc tagttcgccg tctactagtc
     2401 gccagattcc aagcgggttt tcattcttga gttcgtcagg tagtgcgccg ttaggaaagt
     2461 cctggtaaca aacctgacgc gtaaaccgga agatggcgcg cgtgccaacg gatgtcgaac
     2521 gaccgtcaga tgtagccggg taagggccac catgaaccat tgagtgacaa acctcaacgc
     2581 cggttgggaa gccattgaag acaagtcgtc ccactttcgc ttcaagtgct gccaccaatt
     2641 cggagctcga actcatatcc tcgtcattgg catggaccgt cgcggttaat tgcccctcca
     2701 gttggtctat ggctgccagc agctcctgct tattcgaata catgatgact aacgtggatg
     2761 gtccaaacac ctcttcggct aattctggat tagccatgaa ttcttttgcg ttcgtaagaa
     2821 atagagcagt ccgtgcgcca catccttctt gctgttgcgc actggcagta ctcacgagcg
     2881 tttcgactgc catatggcct gaacgcttgg cgacgctttt cttataggta gaacttatgc
     2941 cctcggtgag catcgtttgt tccgggcttt cgctcatcaa agtaccgagc ttttgcacaa
     3001 acgagtgtga ctcttgtgag tcggctaaga atatcaatcc agggttagta cagaactgac
     3061 ctgctcccaa cgtcactgaa ccgcttagac cagccgcaat ttgatccgaa cgttcgctga
     3121 gtgctcctgg aagaatcacc accggattga cactactcat ttctgcataa aatggaatgg
     3181 gctccggtct tgatgatgca gcatcaaaca acgctcgacc acccgctcgc gagccagtaa
     3241 acccgcctgc tttaataagc ggatgtttta ccagcgcggt gcccacgtcg tttcctgagc
     3301 cgtagataag agaaaaaact ccttcaggaa gatcacaact ctgcaccgca tccatcaccg
     3361 ccaaaccaac cagttcagac gttcccggat gagcgtgatg agccttaacg atgac
//
LOCUS       DASSFP010000244         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1839736, whole genome shotgun sequence.
ACCESSION   DASSFP010000244 DASSFP010000000
VERSION     DASSFP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839736"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..936
                     /locus_tag="VFH91_05335"
     CDS             259..936
                     /locus_tag="VFH91_05335"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET6862444.1"
                     /translation="MALRLGDIAPNFTAETTEGPINFHEWIGDGWAVLFSHPKDFTPV
                     CTTELGYVAKSKPDFDKRGVKVVGLSVDPTDSHKRWAEDIKETQGTALNFPVIADPDH
                     KISELYDMIHPEISDVFTVRSVFVIGPDKKVKLMITYPASTGRNFDEILRVIDSLQLT
                     AKYSVATPVNWKDGDDVIIVPSLSDEAAKEKFPGGWRAPKPYLRITSQPNKQTGSTGD
                     TAAKTAS"
     gene            complement(1029..2096)
                     /locus_tag="VFH91_05340"
     CDS             complement(1029..2096)
                     /locus_tag="VFH91_05340"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862445.1"
                     /translation="MSISFKRVVKQFGDTTVVDDLSLDIKDGEFVVLLGPSGCGKTTT
                     LRMLAGLESITSGEIYLGDQLINDVPTQRRDLAMVFQSYALYPHMTIRENIAYPLRVR
                     KLSPDLQKAKVERVAQLLEIETLLERKPRQLSGGEKQRVALARSIVREPRAYLMDEPL
                     SNLDARLRVQMRGELKRLQHQLKTTTVYVTHDQAEAMTLAHRVAVMQKGKLQQFDTPM
                     NIYERPANKFVAEFVGSPSMNFLEGRVDSDSRTFLSGEVKIPLNAAQLRHTGQVVIGI
                     RPEDISITTERSTVESLAAQVYVTELLGNETFVFLMVAGNRLIARTAPDFRADDETPV
                     WLSFAQEKFHFFDSRTGSRIE"
     gene            complement(2172..3932)
                     /locus_tag="VFH91_05345"
     CDS             complement(2172..3932)
                     /locus_tag="VFH91_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HET6862446.1"
                     /translation="MSSCTENSPQMSTNKSVGDADAFMKKAEARLLDLNIKAGRADWV
                     RSTFITEDTENLSAEANDALIAATTELAEQAHSFEGQQLSPDLQRKIKLLKLSLTLPA
                     PKDPAERNELTKLAASMEGEYGKGKYCPDGEKGKCLSLPDLENIMTNSRDPEELKRAW
                     VGWHQIAIPIRKEYVRFVELSNKGAKEMGFKDTGAMWRSKYDMEPDAFSAEMERLWQQ
                     VKPLYDSLYTYTRHKLSEKYGKDVVPEDKPIPAHLLGNMWAQTWGNVYPLLAPANADR
                     GYDLTKILKDKNTDARQMVRYGEGFFTSLGFDSLPQTFWERSLFVKPADREVVCHASA
                     WDIDYEKDVRLKMCININEEDFSVVHHELGHNYYQMAYANQPFLYRDSANDAFHEAIG
                     DTIALSVTPAYLKQLGFIDKVPDQSADIGFLLNRALDKVAFLPFGYLVDQWRWKVFSG
                     EVGPGDYNKAWWELREKYQGVAAPVPRTEQDFDAGAKYHVPANTPYARYFLAAILQFQ
                     FHRALCREAGFNGPLYQCSIYGNKQAGEKLKSMLAMGLSKPWPEALKAMTGEDKMDAT
                     AILDYFAPLKTWLDQQNAAK"
     gene            4148..>4688
                     /locus_tag="VFH91_05350"
     CDS             4148..>4688
                     /locus_tag="VFH91_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6862447.1"
                     /translation="MKKTLQIFTLSVLALTFSNQAVGQTKPQDDFKLTPVANGIFAAT
                     ALPGGLASGNAGFVVGDDGVLIIDTFFTPAAVEELIDAINTQTKQPIKYVVNTHYHLD
                     HTGGNQVFVARGIPILAHDKVIEWQTTRNRKFLPEKDALDKRLDDAQKLLAATPLEQT
                     DKRTQLERQIRRVNAMKSIV"
BASE COUNT         1175 a         1191 c         1143 g         1179 t
ORIGIN      
        1 gtaggttgga ctttaactaa ccaccaactc ccgttggtgg aattctcgga gccacggttg
       61 gttgtcgttg gtaggttgga ctttaacgaa ccaccaaatt caagggaaat ttcgccgttt
      121 cctgcggcgg tttgacttga atggtgctct agctgtaaaa gcgtcttagc ttcctacaag
      181 ttgaatttag atcccttgca atcccggcat ggaagatccc gggatagtca gttttttccg
      241 aaaggaaagg agataccgat ggccctacga ttaggagata tagcgcccaa tttcactgca
      301 gaaaccactg aaggcccaat taactttcat gagtggatag gtgacggctg ggcggttcta
      361 ttttcccacc cgaaagattt caccccggtt tgcactactg aactgggtta tgtcgcgaag
      421 agtaaacctg atttcgataa gagaggcgta aaagtagttg gtttgagtgt cgatcccact
      481 gattctcaca aacggtgggc ggaagatatt aaagagactc agggaacagc attaaacttc
      541 cccgtgatcg cggatccaga tcataagatc tccgaactct atgacatgat tcatccggaa
      601 attagcgacg ttttcacagt tcgctcagta tttgtgattg gcccggataa gaaggtcaaa
      661 ctgatgatta cataccctgc aagtacaggg cgtaacttcg acgagatcct gcgagtgatc
      721 gattcgctac agctcactgc caagtacagt gtcgcgacac cagtaaactg gaaagatggt
      781 gatgatgtaa tcatcgtccc atcactgtct gatgaggctg cgaaggagaa gtttcctggc
      841 ggatggagag ctcccaagcc ttacctgcga atcacttcgc aaccaaacaa gcaaaccgga
      901 tccaccggtg ataccgctgc gaagaccgcg tcttagctac ggttccaagc caacttgcgc
      961 gagcgcaggt caatcgagac ctgcgcttat tttttccaac gctcaaaatt tctataggac
     1021 acaacccctt attcgattcg cgagcctgtt cgcgaatcaa aaaagtgaaa cttctcctgc
     1081 gcaaaactca accatactgg agtttcatca tcagctcgaa aatcaggggc cgtgcgtgcg
     1141 atcagcctgt taccggcaac cattagaaag acaaaagtct cgtttcccaa tagttcggtc
     1201 acgtaaactt gcgcagccaa cgattcaact gtggaccgct cagttgtgat cgagatgtct
     1261 tcgggtcgaa tcccaataac cacctgtcca gtatgacgaa gctgagcagc gttgagcgga
     1321 atcttgacct cacctgacag aaatgtgcga ctgtctgaat cgactctgcc ttcaaggaag
     1381 ttcatgctcg gactacccac aaactccgct acaaatttat tcgccggccg ctcgtaaata
     1441 ttcatcggcg tgtcgaactg ctgtagcttc cccttctgca tcactgctac gcggtgggcc
     1501 agcgtcatcg cttcggcctg atcgtgagta acataaacag tcgtcgtttt cagttgatgt
     1561 tggagtcgct tcagctcgcc tcgcatctgc actcttaaac gggcgtcgag atttgatagc
     1621 ggttcatcca tcaggtaggc gcgcggttcc cgcacaattg aacgagccag cgctactcgc
     1681 tgtttttcac cgcctgataa ctggcgcggc tttcgttcga gcaacgtctc gatttccagc
     1741 agttgtgcga ctcgttcaac cttagctttc tgcaagtcgg gtgatagttt tctaacgcgt
     1801 aagggataag cgatattctc gcgaattgtc atgtgtggat agagcgcata actttgaaag
     1861 accattgcca gatctcgacg ttgagtgggc acgtcgttga tgagttgatc gcccagatag
     1921 atctcacccg atgtaataga ttccagaccg gcaagcattc tgagtgtggt ggttttgccg
     1981 cagcccgaag gaccaagtag tacgacaaac tcgccgtctt taatatcgag cgagaggtcg
     2041 tcgacgacgg tggtatcgcc aaactgtttg acgactcgct tgaacgagat ggacattaat
     2101 cagggtttac actttgtttt tgacgcggcg ctccggctcc gcgtcaaaaa cccgcagatt
     2161 gagaggcgcc actatttcgc ggcgttttgc tggtcaagcc aggtcttcaa gggtgcaaag
     2221 tagtccagaa tcgccgtggc atccatctta tcttcgcccg tcatggcttt aagagcttcg
     2281 ggccatggtt tactcagacc catcgccaac atcgatttca gtttttctcc agcctgtttg
     2341 tttccataaa tcgagcattg gtaaagcgga ccattgaagc cagcttcacg acaaagagct
     2401 cgatgaaact ggaactgcaa gatcgcagcc agaaaataac gcgcataagg agtgttagcc
     2461 ggaacgtgat atttggcgcc cgcatcaaaa tcctgttccg tacgcggaac cggcgcggct
     2521 acgccctgat acttctctcg caattcccac catgctttgt tgtagtcgcc tggtccaacc
     2581 tcgccggaaa agaccttcca gcgccattgg tccacgagat atccaaacgg taagaacgca
     2641 accttatcaa gcgctcgatt caaaaggaac ccgatatcag ctgattggtc tggaacttta
     2701 tcgatgaacc caagctgctt tagataagct ggcgtaacgg aaagagcgat ggtatctccg
     2761 atggcttcat ggaatgcgtc attggcgctg tcgcgataaa gaaaaggctg attggcatag
     2821 gccatctgat agtaattgtg gcccaactcg tgatggacga cgctgaagtc ttcctcgttg
     2881 atgttgatgc acatcttcag ccgcacgtct ttttcgtagt cgatgtccca cgcacttgca
     2941 tgacaaacaa cttcgcgatc ggcaggcttc acgaataacg atcgttccca gaatgtctga
     3001 ggaagtgaat cgaaaccgag agacgtaaag aaaccctcac cataacggac catctgcctg
     3061 gcgtccgtat tcttgtcctt gagaattttt gtcaggtcgt aaccacgatc ggcattcgca
     3121 ggcgcaagca atggataaac attaccccac gtttgggccc acatatttcc aagcaagtgt
     3181 gccggaatag gtttatcttc aggtacaaca tctttgccgt acttctctga tagtttgtgg
     3241 cgcgtgtacg tatagagcga gtcgtagagt ggcttgacct gttgccagag tcgctccatt
     3301 tcagctgaga aagcgtcagg ctccatatca tacttggaac gccacatcgc accggtatcc
     3361 ttgaacccca tttctttagc gcccttgttg ctcaactcga cgaaccggac gtattccttt
     3421 cgaatgggga tggcgatctg atgccacccg acccaggctc gtttcaattc ctctggatca
     3481 cgactgttgg tcatgatgtt ttccagatcc ggcaagctga ggcacttccc tttttcaccg
     3541 tcaggacaat atttgccttt cccgtattcc ccctccatcg acgctgcaag cttggtgagc
     3601 tcatttcgtt cggccggatc ttttggtgct ggcaaagtta gcgataattt cagcagttta
     3661 attttgcgct gcaaatcagg cgacagttgt tgtccttcaa atgagtgggc ctgttcggct
     3721 aattcggttg tggccgcaat caaagcgtcg ttggcttcgg cggaaagatt ctcagtatcc
     3781 tcggtgatga aggtgcttct gacccaatcg gctcgcccgg cttttatatt gagatcgagt
     3841 aaacgagctt cggccttctt catgaacgcg tcagcatcac caacagattt attggtcgac
     3901 atttgcggtg agttttcggt acaggatgac atgagtgaga ttcctataat gagagtgacg
     3961 aaaacaccaa cccagaaccg gctggattga gaactaagtt tcacgttgag aaacctccac
     4021 gtttctggta ttggactgat ttgcggaatg ggctctgtca ctatagcgag gatttctgcc
     4081 tggctcaatt ggggaagaat aggattatag ggcctatagg acttatagga ccgattcact
     4141 ataaaaaatg aaaaaaacac tccagatctt cacactctcc gtcctggcat tgacgttttc
     4201 caaccaggct gtcggtcaaa caaaaccaca ggacgacttc aaactaacgc cggttgcgaa
     4261 cggaatattc gctgcgactg cgttgcccgg tggactggcc tccggaaatg ccggattcgt
     4321 cgtgggcgac gacggtgttc taatcatcga tacatttttt acccccgctg ccgtcgaaga
     4381 actcattgac gcaatcaaca ctcaaacaaa acagccaatc aaatatgtcg tgaataccca
     4441 ttaccattta gaccacaccg gtggtaatca ggtgtttgta gcccgcggca ttccaatcct
     4501 ggcccatgac aaagtgattg agtggcaaac aaccaggaat cgcaagttcc tgccagaaaa
     4561 agacgcatta gacaagcggc tcgacgacgc gcaaaagctg ttggctgcaa ctcccctgga
     4621 acaaacggat aagcggactc aactcgagag acaaattcgt cgtgtaaacg cgatgaagtc
     4681 gatcgtgt
//
LOCUS       DASSFP010000245         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1839769, whole genome shotgun sequence.
ACCESSION   DASSFP010000245 DASSFP010000000
VERSION     DASSFP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839769"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VFH91_05355"
     CDS             complement(<1..1303)
                     /locus_tag="VFH91_05355"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET6862448.1"
                     /translation="MSQRLTIVLTGVAVIGVLVILNTLTYVKSDKSHDFEVTPNRSTY
                     HPGPTGTLALYDLLNESGRQVMRWRETPGKLLKQPGSSVTTFVVIGPTRIEFSKDDVR
                     ALLQWVEQGGRLVLIDRGPDRSLLPRSEHWNVSPVQMDYPTLDVDPSDTKQMTERVVA
                     LQPVQPTLLTRDVQAVIPSRFASRIRIDSVGKPDEEGFDDENGASESSRAPVIHIADQ
                     TGGLLVDYAHGNGTITLLTDPYIVANGGITLRDNLQLAMNTIAPYQGLIAFDEYHQGR
                     GLTENPIAGYFSGTPVLALAAQLAALVIFLIWTRARRFGRPLPLPHTDRRSTLEFVAS
                     MSEIQERAKAYDLAIENIYSRTRRVLARYAGVDYNSPRSVIAARVAARSSIDQRRLET
                     LMQQCENTINGEPTSWRQAIDLVKRLREVEQTLGLGMRSRDM"
     gene            complement(1310..2341)
                     /locus_tag="VFH91_05360"
     CDS             complement(1310..2341)
                     /locus_tag="VFH91_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET6862449.1"
                     /translation="MSFKWCVTAFLIICFAAVAHAIPLSEYQNNLKRAVTALDTLSQV
                     DENETEADFQNRAGETATILKSLIPVTQPVENGNETCTVYNLWFHQELDDYLRTQPDL
                     RPDKLSMILERVKAVEDRVAELQKPRLPGMSKDESKERLSTILSRPEFSHQSSSQTAL
                     NKLLQEFIRWIQSWFPKMKPLEPGRAYWVSRVAQIVVVILATALIAFVVFQLLSQAKR
                     KKATKKKTKRGPLIVLGEELQPEDSATDLLSEAEALARKGELRAAIRKAYIALLVELG
                     DRKILHLAQHKTNRDYLKSVSNLPVLHSYMSGMTESFERHWYGLAQATVDDWQNFRQF
                     YYAARSAKG"
     gene            complement(2365..>2600)
                     /locus_tag="VFH91_05365"
     CDS             complement(2365..>2600)
                     /locus_tag="VFH91_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862450.1"
                     /translation="LGPLSSILLTPVWMLGLSLLYVDERVRHEGYDIELLAAKYLGEV
                     PDSNVASPLGPALAVNVPKPPPPPVHRGILNLG"
BASE COUNT          611 a          667 c          654 g          668 t
ORIGIN      
        1 gcatatctct ggaacgcatg cccagaccaa gagtttgttc cacttcccgc aaacgtttta
       61 ctagatctat ggcctgtctc caactagttg gctcaccatt gattgtgttt tcacactgct
      121 gcatcagggt ttccaatcgt cgttgatcga tactcgaacg agctgccacc ctcgcggcga
      181 ttactgaacg aggactgttg tagtcgacgc cggcatagcg cgcgagcact cggcgagttc
      241 gcgagtagat attctcgatc gccaggtcat aagccttcgc acgttcctga atttctgaca
      301 ttgatgccac aaactcgaga gtagaacggc gatcggtatg gggcaaagga agtggtcttc
      361 caaagcgtct cgccctggtc caaatcagaa aaataaccag agcagcgagt tgggcggcca
      421 aggcgagcac tggcgttccg gaaaaataac ccgcgatagg attttccgtt aggcctcttc
      481 cttgatgata ctcatcaaaa gcgatcaagc cctggtacgg cgcaattgta ttcatagcaa
      541 gttgcaaatt atccctgagc gttataccgc cattagcaac tatgtacgga tcggttaaca
      601 aagtgatcgt gccatttcca tgtgcgtaat caacaagcag acctccggtt tgatcagcta
      661 tatgaataac cggcgcgcga gacgattccg aagctccatt ttcgtcatca aatccctctt
      721 catcaggttt tccaacggaa tcaattctga tacgcgaagc aaaacgtgag gggatcaccg
      781 cctgaacgtc ccttgtgagt aaggtcggct gcacgggctg cagtgctacg actcgttcgg
      841 tcatttgttt ggtgtcagac ggatcgacat ctaaagtcgg ataatccatt tgcaccggac
      901 taacattcca atgctcggat cgcggcagca acgagcgatc tggacctcga tcgatcagaa
      961 ccagccgtcc tccctgttca acccactgca agagcgcacg cacatcatct ttactgaatt
     1021 cgatccgggt tggtccgatg acgacgaagg tagtaacgct cgagccaggc tgcttgagca
     1081 gtttgcccgg agtttcgcgc cagcgcatca cctgccggcc cgactcattg aggagatcgt
     1141 agagcgcgag agtaccggtg ggaccaggat ggtaggttga tcggtttggc gtaacttcga
     1201 agtcatgcga cttgtcgctc tttacgtaag taagcgtatt caggatcacc aacacaccga
     1261 tgacggcaac tccagttagg acgatggtca gacgctgact cattgactct tagcctttag
     1321 cgctccgggc ggcgtaatag aactgtcgga agttttgcca gtcgtcgact gttgcctggg
     1381 ccaatccgta ccagtgtcgt tcgaaactct cggtcatacc agacatgtaa gagtgcagca
     1441 ccggtagatt gctgactgac ttcaaatagt cgcgatttgt tttatgttgg gccaggtgca
     1501 aaatcttcct atcacctaat tccaccagca gagctatgta agccttgcgg attgcggccc
     1561 tcaactcgcc cttacgagca agtgcttcag cttccgataa aaggtcggtg gctgagtctt
     1621 ctggttgcag ttcttctccc aggacgatga gtggaccacg cttcgtcttc ttcttagttg
     1681 ccttcttacg ttttgcctga cttagaagct ggaatacgac aaaagcaatg agcgctgtgg
     1741 ctagtatgac taccactatt tgcgcaaccc gactgaccca ataagcacgt ccgggctcca
     1801 gcggtttcat tttcgggaac cagctttgaa tccaacgtat aaactcttgt aaaagtttgt
     1861 tgagagcggt ttgactactc gattgatgcg aaaattccgg ccggctgagg atcgtggaca
     1921 atctctcttt cgactcgtcc ttagacattc cgggcagcct cggtttctgt agttcggcaa
     1981 cgcgatcttc gactgctttt actcgttcca atatcatcga gagcttgtca ggtctgagat
     2041 ctggttgggt gcgcaggtag tcgtcgagtt cttgatgaaa ccagaggttg tagacggtgc
     2101 aagtctcatt tccattctca actggctggg ttacagggat caggctcttc aaaatcgtcg
     2161 cagtttcgcc ggcgcgattt tggaagtcgg cttcagtctc gttttcatcg acctgactaa
     2221 gcgtatctaa ggctgtaact gcccgtttta gattgttctg gtactcacta agtgggatag
     2281 cgtgagcgac agcagcaaag caaattatga ggaacgctgt gacgcaccac ttaaaactca
     2341 ttcttatttg ctgtttgctc tgcatcaacc caggttcaag attcctcgat ggaccggcgg
     2401 tggtggcggt ttgggtacat ttacggccag agctggtcca agcggcgacg cgacatttga
     2461 atcagggact tctcccaaat atttggctgc taacaactcg atgtcatatc cttcgtgccg
     2521 cactcgctcg tccacatata gtagcgacaa accaagcatc catacgggtg ttaaaaggat
     2581 tgaactcaaa ggtcctagaa
//
LOCUS       DASSFP010000246         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_367574, whole genome shotgun sequence.
ACCESSION   DASSFP010000246 DASSFP010000000
VERSION     DASSFP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367574"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /gene="menC"
                     /locus_tag="VFH91_05370"
     CDS             <1..784
                     /gene="menC"
                     /locus_tag="VFH91_05370"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET6862451.1"
                     /translation="LDVPLWRHLGGTRDEISCGVSIGIQDSPEILLEKIRKEVDAGYQ
                     RIKIKIKPGWDLRIVERVRHEFPEIRLMGDANSAYTLGDVKLFKELDEFDLMMIEQPL
                     AHDDIFDHAKLQRQIETAVCLDESIHSAEDARHALELGSCKIINVKLGRVGGHAEARR
                     VERVARENDIPVWCGGMLESGIGRAHNIAMSTLSGFTLPGDVSASSRYWAQDIIEPAV
                     TVSKRGTISVPEEPGIGFKVDLGRIEAFTVRKETLNSNSPIL"
     gene            781..1950
                     /locus_tag="VFH91_05375"
     CDS             781..1950
                     /locus_tag="VFH91_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET6862452.1"
                     /translation="MTIISDEKIRQIHAALSSRQAEIEKFVQELVELESPSGDETGSR
                     EVVAALVSAAERLNCITSIEKVDVDKFGRHVLIRAFQDEKPGQLLVVGHTDTVHARGS
                     VRRRPWRREAGKIFGPGIFDMKGNCALAIEVFRTLEELKIEPQRGVALILTCDEEVGS
                     MSGWPFIERVARSSETAFAMVLEPPASGGRVKTGRKGTGIFTLKVEGRAAHAGLEPEK
                     GASAILELARQTERIHSINKAGAGITVNVGVVRGGTRSNVVPAEAEAEIDVRFSNERE
                     AEEIEQFLLNLRPIDERTRVFVSGGINRPPLERTPAVVSLFEKARSIAKVVNFDLGDA
                     QVGGASDGNFLAALGIPVLDGLGINGDGAHADHENIVAADIPIRGTLIAGLIASA"
     gene            1975..2742
                     /gene="xth"
                     /locus_tag="VFH91_05380"
     CDS             1975..2742
                     /gene="xth"
                     /locus_tag="VFH91_05380"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET6862453.1"
                     /translation="MLVATWNVNSILARIPNVIRWLDLNKPDVLCMQETKCTDDKFPL
                     EPFTERGYHCQLFGQQSYNGVAIVSRGPCETGYRGYPGDDETAQSRLIATTVNGIRVI
                     NVYVPNGQMVGSEKYAFKLEWMKRLRSFFDSNYDLQTPVLLCGDFNVAPEEIDVYDPR
                     LWKGRILFSDQERAALQYIKDWGFTDSFRLHNSLPGQYSWWDYRAGAFRRNLGLRIDH
                     IWISNSLVERNVKTWIDIEPRKWEKPSDHAPVIAEFS"
     gene            complement(2735..>3032)
                     /locus_tag="VFH91_05385"
     CDS             complement(2735..>3032)
                     /locus_tag="VFH91_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862454.1"
                     /translation="YSRFARYPEAEAALELAVYAEPDSIKARSFLGVTNYLRGNVSGA
                     MEQYHWVLGKDPKIAAEMLKGYQEIADKMNEVQNKKQPVKNGPKKDFAQQKSLS"
BASE COUNT          749 a          706 c          818 g          759 t
ORIGIN      
        1 tctcgacgtt ccattgtggc gccatcttgg tggcactcga gatgaaatct cctgtggagt
       61 ctcgattggg atacaggatt ctcctgaaat cctgctcgag aaaattcgca aggaagtcga
      121 tgccggttat cagcgaataa agatcaagat caaacctggc tgggacctga ggattgtaga
      181 gcgagtgcgt catgagtttc ctgagatccg gttaatgggt gacgcaaact cagcttacac
      241 actgggtgat gtgaagttgt tcaaagagct cgacgaattc gatctgatga tgatcgaaca
      301 accattagct catgacgaca tattcgatca tgcgaaactg caacggcaaa tcgaaacagc
      361 cgtttgcctt gacgaatcga ttcactccgc cgaagatgcg cggcatgcgc ttgaattggg
      421 ctcctgtaaa atcataaatg tgaaactagg gcgtgttggt ggtcacgctg aggctcgcag
      481 agttgagcga gtagcgcgcg aaaatgacat tcctgtatgg tgtggtggaa tgctcgaatc
      541 gggcatcggg cgtgctcata acatcgcaat gtcaacgctt tctggtttta ccttgcccgg
      601 tgacgtctca gcaagctcgc ggtattgggc gcaagacatc atcgaacccg cagtcacggt
      661 cagcaagcga ggaacaattt cggttcccga agaacccggt atcggtttca aggtcgacct
      721 gggacgaatt gaagcgttca cggtacgaaa agaaactcta aactccaatt cgcctatcct
      781 atgactataa tttccgatga gaaaattcgg cagattcacg ctgcgctgag ttcaaggcaa
      841 gctgaaatag aaaagtttgt acaagaactt gtcgaactcg agtctccttc tggtgatgaa
      901 accggcagtc gagaagtcgt tgctgccttg gtgtctgcgg ccgagcgtct caactgcata
      961 acgtcaatcg aaaaagtaga cgttgataag tttggccggc atgtattgat tagggccttt
     1021 caagatgaaa aaccgggcca gctcttagtg gttggacata ccgacactgt tcacgcccgg
     1081 ggctcggttc gccggaggcc gtggcgacgt gaggcaggca agatttttgg tccaggcatc
     1141 tttgacatga aaggtaactg tgctctggcc attgaagtct tccgcaccct ggaagagcta
     1201 aagatcgaac ctcagagggg cgtggcactg atattaacct gcgacgaaga agtgggcagt
     1261 atgtctggtt ggcccttcat tgaaagagtg gcgcgttcga gtgagactgc gtttgctatg
     1321 gtgttggaac ctccagcttc gggcggtcgc gtgaagacag gccgcaaagg cactggaatt
     1381 tttacgctta aggttgaggg aagagcagct cacgccggac tcgagccgga aaaaggagcg
     1441 agtgcgattc tcgagctggc caggcagaca gaacgaattc actcgatcaa taaggcggga
     1501 gccggaatca cggttaatgt tggtgtcgtc cgtggtggaa ctcgatcgaa tgtcgttcct
     1561 gccgaagcag aggcagagat tgatgtgcgc ttttcgaatg aaagagaagc agaggaaatt
     1621 gaacagtttt tacttaacct gcggcccatc gatgaacgaa caagagtctt cgtttcaggg
     1681 gggattaatc gtccgccgtt ggagcgaact ccggctgtag tgtcactctt cgaaaaagct
     1741 cggtctatcg caaaggttgt aaatttcgat ctcggtgacg cacaagttgg tggtgcttcc
     1801 gatggtaatt tcctcgccgc gctgggaatt cctgtgcttg acggtcttgg tatcaatggc
     1861 gatggagctc acgcggatca tgaaaacatc gtagcggccg acattcccat acgagggact
     1921 ttaatagccg gccttattgc ttcagcctga ttggaccatc tcgattcgac acacatgcta
     1981 gttgcaacct ggaacgtaaa ctcaatactc gctcgtatcc ctaacgtcat tcgctggctg
     2041 gatctaaaca agcctgatgt actttgcatg caggagacaa agtgcaccga cgacaagttt
     2101 cctttggagc cttttacgga acgcggctat cactgtcagt tgtttggtca gcagtcgtat
     2161 aacggagtgg ctattgttag tcgtggtcct tgtgagacgg gctatcgagg ttatcccggc
     2221 gatgatgaaa ctgcgcagtc acgattgatc gctaccaccg taaatggaat acgcgtcatc
     2281 aatgtctatg tcccaaatgg acaaatggtg ggttcagaaa agtacgcatt caagctcgag
     2341 tggatgaagc ggctgcgctc cttttttgac tcgaactatg atctccagac gccggtctta
     2401 ttgtgtgggg acttcaacgt cgctcctgaa gagattgacg tttacgatcc acgcttatgg
     2461 aagggtcgaa tccttttcag cgatcaggaa cgggcggcgc ttcaatacat aaaagattgg
     2521 ggctttacag attcgtttcg tctgcataac tcattgcccg gacaatactc atggtgggac
     2581 taccgggccg gagcctttcg tcggaacctt ggactgcgga tcgatcacat ctggataagt
     2641 aattcgttgg ttgaaaggaa cgtcaaaacc tggatcgata ttgaaccgcg taagtgggaa
     2701 aagccttcag accacgcgcc tgtgattgcg gagttcagct aagtgatttc tgttgggcga
     2761 aatccttctt tggcccattc ttaactggct gctttttgtt ttgcacctca ttcatcttgt
     2821 cagcaatctc ttgatagcct ttcagcatct ccgccgcaat ttttggatct ttccctaaga
     2881 cccaatgata ttgctccata gcaccactga cgttgccacg aagatagtta gttactccca
     2941 aaaacgatcg cgccttaatt gaatcgggct cagcataaac tgctaattcg agcgcggctt
     3001 cagcctccgg atatcttgcg aaccgagaat ac
//
LOCUS       DASSFP010000247         6283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1553351, whole genome shotgun sequence.
ACCESSION   DASSFP010000247 DASSFP010000000
VERSION     DASSFP010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553351"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..489)
                     /locus_tag="VFH91_05390"
     CDS             complement(22..489)
                     /locus_tag="VFH91_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862455.1"
                     /translation="MPNARWRRYLRIAIVVGCIAQLSSGYVDAQNKRDKFDPDGAFWI
                     HGQPPNEFSDFSAINLNAKRVRRLPAAGLQLNNGKNFRFKTLSTKPQELAFTTLVIAG
                     VSYSFSGKFLKTGVFATGMLDDETPVLEGTLTKFKAGKKVAEANLKFVYFGGT"
     gene            complement(482..985)
                     /locus_tag="VFH91_05395"
     CDS             complement(482..985)
                     /locus_tag="VFH91_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET6862456.1"
                     /translation="MSNSALPEQRSLILNLNELLERVNRYREKGASVVLANGCFDVLH
                     VGHVRYLTAAKELGDVLVVAINSDEQVRLLKGEGRPVMSAAERAELISSLEPVTFVTV
                     FNEPTVKELLLALEPDFHAKGTDYTEETVPERDVVRSYGGKVAIVGDPKNHSTSKILS
                     QLGGDDA"
     gene            complement(982..2001)
                     /locus_tag="VFH91_05400"
     CDS             complement(982..2001)
                     /locus_tag="VFH91_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HET6862457.1"
                     /translation="MSTETTTRHKLAEVIPQFANCRILIIGDAIADRFVYGNISRVSR
                     EAPVFILRHEHTETVPGGAANCAVNVAALGAKVSLLSVIGNDEPGQQLRSKLAAAGVN
                     TSTLLVSDGWRTTTKERILAGQAHSTKQQVIRIDYEGTQLPDESLRESILESINAEVA
                     NCDAVIISDYNYGVVDDQAIEAIRKTTRANPLPVLVDSRFRLSTFTGFTAATPNEDEL
                     EQLIGEAIQDQDHLEECARQLNKALNAEALLVTRGGQGMMLIERNHEARHIPAVGAAQ
                     PVDVTGAGDTVIATFALAIASKASFLEAASLANYAGGLVVMKRGTASVSARELQESIL
                     SVENE"
     gene            complement(2009..2578)
                     /locus_tag="VFH91_05405"
     CDS             complement(2009..2578)
                     /locus_tag="VFH91_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6862458.1"
                     /translation="MSQPAVFIDRDGTISEEVGYVNHPSRYRVFPYAAAAIELLHTAG
                     WLAIVVTNQAGVARGYFSEELVAKVHQELTRQLESNGSQLDAIYYCAHHPSVGEPPYR
                     QDCDCRKPKPGLIQQASRDFDIDLKSSWMVGDRYSDIEMAHNAGLKAAFVLSGYGRGE
                     WEYQRSAWVHQPDLVCENLLQAVQSIIGE"
     gene            complement(2585..3682)
                     /gene="waaF"
                     /locus_tag="VFH91_05410"
     CDS             complement(2585..3682)
                     /gene="waaF"
                     /locus_tag="VFH91_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET6862459.1"
                     /translation="MSGKQNSDIQRVVVRGTNWVGDCVMTIPALRALRRVLPNAHITL
                     VVRPGVAELFEAANVVDEILRYDRRNALSVFSQVREWRRHKFQLAILFQNAFEAALIP
                     LLAGVPARLGYATEGRQKLLNHPLPVPDWKNDKHEVFYYLYLIAALEQSLYGTSKILE
                     ADPDASIEVPEIKKTSARQFLDTFGLISKKGLVALCPGSINSRAKRWPAERFAALADR
                     LIEDDRTVLLIGSPAEQEVSTLVVSTMKHKPIVLTGKTSLDQITSILSLADLLVTNDT
                     GPAHIAAALNTPAITIFGPTNPLTTRPFSLQAEVLRHPPDCAPCMLRDCPIDHRCMTA
                     ITVEEVFDHAFAILKRRSFNNTVRSLCDSVA"
     gene            complement(3686..4528)
                     /locus_tag="VFH91_05415"
     CDS             complement(3686..4528)
                     /locus_tag="VFH91_05415"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6862460.1"
                     /translation="MKILVTGGSGYLGTHVRRFFSADDFSRRSNLDVLNIYDAQKASD
                     YDVVIHLAAHLDKNPKAADQCFRTNAEGTLNLIRNMQPGSVFIYASTKDVYGPHAGTF
                     SDVPETCSTEYADQSALEWSKLIGEKYVSYYAAKQNVRSCIFRLSAVYARPSSGNDHG
                     FVTHYVESVKHSRPIHIPANVNPVRDILYVDDFSRACQGFIDSSSESGLFNLGGGRGN
                     AASLREIITRIGTLIDLQPLIEEDEKIPIPVPLNYISDLTHVKAKLGWEPQVGIDEGL
                     RNLL"
     gene            complement(4539..5597)
                     /locus_tag="VFH91_05420"
     CDS             complement(4539..5597)
                     /locus_tag="VFH91_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HET6862461.1"
                     /translation="MRSRSSEPFNFRPNRTRAKVGIAAVLVAITVGTIVFHELEGWTI
                     LDSLYVTAQTVTTVGFGDLAPRTRAGRAFATVFMMVGVGIVLYALTSTVQSIVQSELL
                     ATFGKRRYSRKMSRLRNHFIICGAGRVGSHLVRSLKGSEHPFIVIESDPKKVEELMDL
                     GIAVLVRDATLEESLRDAGVEYARGLAACLPDDANNVYVVLTARDLNPKLHIVARAAE
                     EQAEHKLIRAGANRVVAPTIIGGHRMAMALSKPAVEDFLDSITANELDLGFEQLEVGP
                     TSQLIGRKLRETNIRSELNIVVVSIKRIDGEIIFNPSGETLVQNGDMLIAIGSAETLT
                     KLKALAHGESAPSGLVRA"
     gene            5860..>6283
                     /locus_tag="VFH91_05425"
     CDS             5860..>6283
                     /locus_tag="VFH91_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862462.1"
                     /translation="MRRLFSLSVLIPLVFLGAVVLGVLIYYWTVFSARIDTLLQGEVY
                     TKSAGIYAAPKQLRVNELISQDEVVAFLKRAGYVEKDQQADSSRGRYQSGVNTLDIEP
                     SGTSTVDGRQQFQKVRVQFAKTGKSIANLTDIDGRANLQ"
BASE COUNT         1532 a         1608 c         1560 g         1583 t
ORIGIN      
        1 tcgcatcagc gtcttaaatt ctcaagtgcc cccgaagtaa acaaatttca ggttagcctc
       61 tgcaaccttc tttcccgcct tgaatttagt caaggtacct tccaggacgg gcgtttcgtc
      121 gtcgagcatg ccggttgcaa agacaccggt tttcaaaaat ttcccagaaa aactgtaaga
      181 gacgccagca atcaccaaag tcgtaaatgc cagttcctgt ggtttggtgc ttagtgtttt
      241 aaaacgaaag ttctttccat tgttcagctg aaggcctgca gccggaagtc tacgaacccg
      301 cttcgcgttt aaatttatag cgctgaaatc cgagaattcg tttggcggct gaccatggat
      361 ccaaaacgcg ccgtcagggt caaacttgtc cctcttattt tgcgcgtcga cataaccact
      421 gctcaactgc gcgatgcacc caactacgat cgcgattcgt agatatcgtc tccatcgagc
      481 gttaggcatc gtctcctcca agttgagaca aaattttgga tgttgagtga ttttttggat
      541 cgcccacgat ggctaccttg ccgccgtatg atcgtaccac gtctctctca ggtacagttt
      601 cttcagtgta gtcggtccct tttgcgtgaa agtccggctc aagagctagc agtaactctt
      661 tgactgttgg ttcattgaag acggtgacaa atgttacagg ctccagagat gagatcagtt
      721 ccgctctttc agcagcgctc ataacgggtc ggccttcgcc cttcagcagt cgtacctgtt
      781 catccgagtt tattgcaacc accaggacat cgcccaactc tttcgcagca gttagatatc
      841 taacgtgtcc cacatgcaac acatcaaaac aaccgtttgc caggactaca gatgcgccct
      901 tttcgcgata tcggttgaca cgctcgagca actcgttgag attcaggatt aacgaccgtt
      961 gctccggtaa ggcgctgttg ctcattcgtt ttctacactc aaaatagact cttgcagttc
     1021 gcgagcagaa actgaagcgg ttccacgttt cattacaaca aggcccccag cgtaattcgc
     1081 gagtgaagca gcctccaaaa acgatgcctt acttgcaatc gcgagggcaa aagttgctat
     1141 gaccgtatca cccgctccag tgacgtcgac aggctgtgcc gcgcccactg cgggaatgtg
     1201 ccgagcttcg tgattcctct caataagcat catcccttgt ccgccccggg tcactagcag
     1261 cgcttctgca ttcaaagctt tgttaagttg tcgtgcgcac tcttccagat gatcttgatc
     1321 ttgaatagct tcgccaatca gttgttccaa ctcgtcttca ttcggagtcg cggccgtaaa
     1381 acccgtaaaa gtggaaagac gaaatcttga atccaccaga acgggaaggg gattggcacg
     1441 agtcgttttg cgaatggctt cgattgcctg atcatcaacg actccgtagt tatagtcaga
     1501 gatgatgacc gcatcacaat ttgcgacttc ggcgttgatg gattcgagga ttgattctcg
     1561 caggctttcg tctggtaatt gagtgccttc gtaatcgatg cgaatgactt gctgtttcgt
     1621 cgaatgagcc tggccggcaa gaattcgctc cttcgtcgtc gttcgccagc cgtccgagac
     1681 gagcagggta gaggtgttca cacccgcggc agctaacttg cttcgcagtt gttggcccgg
     1741 ttcgtcatta ccgatcacag agagcaacga gaccttagct ccgagtgcgg cgacatttac
     1801 cgcacaattt gcagcgccac cgggaaccgt ttcagtgtgt tcgtgacgca gaatgaaaac
     1861 aggagcctct ctagagaccc gactgatgtt gccataaaca aatctgtccg cgatcgcatc
     1921 gccgataatc aagattcggc aattggcaaa ctgcggaatg acttcggcga gtttgtgtcg
     1981 ggttgtagtc tcggtactca aagtttcttc actctccgat gattgactgt acggcctgca
     2041 atagattctc gcaaactagg tcaggttggt ggacccaggc cgatcgttga tactcccatt
     2101 ctcctcggcc ataaccactc agaacaaacg ccgcttttaa tccggcattg tgggccatct
     2161 caatgtcgct gtaacgatct cccaccatcc agctggattt taagtcaata tcaaaatccc
     2221 gggacgcttg ctggataagg cctggcttcg gtttacgaca gtcgcaatcc tggcggtaag
     2281 gcggctcgcc gacgcttggg tgatgcgcac aataatagat ggcatccagt tgtgagccat
     2341 tactttcaag ttgtcgggtc aactcctgat gaacctttgc gaccaattct tcagagaagt
     2401 acccgcgagc aactccggcc tggttcgtaa ccacaattgc aagccaacct gcggtgtgaa
     2461 gcagttctat agctgcggcc gcatatggga agacgcgata tcttgacgga tggttgacgt
     2521 agccaacctc ttcagagatt gttccatctc gatctataaa gactgcaggt tgagacataa
     2581 tttcttaagc taccgagtcg caaagactcc gaacagtgtt gttgaaactt cttcgtttaa
     2641 gaatagcgaa cgcgtggtca aacacttcct cgactgttat ggcggtcata cagcgatggt
     2701 cgatcgggca atctcgcaac atacaaggag cgcagtcagg cggatgacgt aaaacttcag
     2761 cttgcagcga aaaaggacgc gtcgttaatg ggttagtggg accaaagatc gtgatagcgg
     2821 gagtatttag tgccgcggca atatgagctg gtccagtgtc gtttgtaact aaaagatcag
     2881 ccaggctgag gatcgacgta atctggtcca ggctcgtctt gcctgtaagt acaatcggtt
     2941 tatgtttcat cgtacttacg accagtgtgg agacttcttg ttccgctggc gatccaatta
     3001 atagcacggt gcgatcatct tcgatcagcc gatcggcgag ggctgcgaat ctctctgcgg
     3061 gccaacgttt agcgcggcta tttatggaac cgggacaaag tgcgaccaat ccctttttcg
     3121 agatgagacc aaacgtatca agaaactgcc gggcactggt tttcttaatc tccgggacct
     3181 caatgcttgc gtctggatca gcctcaagga tcttgctcgt cccataaagc gattgctcta
     3241 acgccgctat caggtacaga taataaaaga cttcgtgttt atcgttcttc caatccggaa
     3301 cgggcaatgg atggttgagc agtttttgac gcccctcggt cgcgtagccg agtcttgcgg
     3361 gcacgcccgc cagcaacggg atgagcgccg cttcgaaagc attttgaaac agaatggcga
     3421 gttggaattt atgacgtcgc cattcgcgaa cctgcgaaaa caccgacaaa gcattacggc
     3481 gatcgtagcg aaggatctcg tccaccacgt ttgccgcctc aaataactcg gcgacgcccg
     3541 gcctgaccac aagggtgata tgagcattag gtaagactct tcggagcgct cgcaacgcag
     3601 ggattgtcat gacgcagtct ccaacccagt tggtcccgcg gacgacgaca cgttgaatat
     3661 cggagttctg cttaccgctc attctctata acaagttcct taggccttcg tcgattccta
     3721 cctggggctc ccagcctaac ttcgctttta cgtgagttag atctgagatg tagtttagcg
     3781 gaacaggaat gggtatcttt tcgtcttctt cgattagggg ttgaagatct atcaatgtgc
     3841 cgatgcgtgt gattatttcg cgcaacgaag cggcattacc tcggccaccg cccaggttga
     3901 aaaggccgga ttccgatgat gaatcaataa agccctgaca agctctcgaa aagtcatcaa
     3961 cgtaaagaat gtcgcgcacc ggattcacat ttgcgggaat gtgtatgggc cgactgtgct
     4021 tcaccgactc gacgtaatga gtaacgaagc catggtcatt gcctgaactt ggacgcgcat
     4081 agactgcgga tagcctgaat atgcaggaac gaacattctg ttttgcagcg tagtagctga
     4141 catacttctc gccaatcaat tttgaccact ccaatgccga ctggtcagca tactcagtcg
     4201 agcatgtttc gggaacatcc gaaaaagttc cagcgtgtgg tccataaaca tctttcgttg
     4261 aggcatagat aaaaaccgaa cccggctgca tgttgcgaat gaggttaaga gttccctcgg
     4321 cgtttgttcg gaaacattga tcggcggctt tgggattttt atcgaggtgc gcagcaagat
     4381 ggattacgac atcgtaatcc gaagccttct gcgcatcgta aatgtttaga acatcgaggt
     4441 tggaccgtct ggagaagtcg tcagctgaga aaaagcggcg aacgtgtgtt cccagatagc
     4501 cgctgccgcc ggttacgaga attttcattc aattaatatc acgcacgcac gaggccactt
     4561 ggtgcggact cgccgtgagc taaggctttc aactttgtca gggtttcggc acttccaatc
     4621 gcgatcaaca tatcgccatt ttgcaccagg gtttcaccgc ttgggttgaa tatgatttct
     4681 ccgtcaatac gtttgatgct tacaaccacg atattgagtt ctgagcgaat gttagtttcg
     4741 cgcagtttgc gaccaatcaa ttgtgacgta ggcccgactt caagttgctc aaaaccgaga
     4801 tcgagctcat tcgcagtaat tgagtccaga aaatcttcaa cagcgggctt ggataaagcc
     4861 atggccatac gatgaccgcc aatgatcgtt ggcgccacaa cacgatttgc gccagcacgt
     4921 ataagtttat gctcagcttg ttcctccgcg gctcgcgcaa cgatatggag cttcgggttt
     4981 aagtctcgag cggtcaagac cacgtaaaca ttatttgcgt cgtccggaag acaggccgcc
     5041 aaaccacgag cgtactctac gccggcgtca cgtaggctct cctcgagagt ggcatcacga
     5101 actagtacgg cgatgccaag atccatcaat tcttcgacct tcttaggatc actctcaatc
     5161 acgatgaagg gatgttcaga ccccttcagg ctgcgcacga gatgagaacc gactcgacct
     5221 gcgccgcaga tgataaaatg gtttcgcagt cggctcattt ttcgtgaata gcgccgcttg
     5281 ccaaaagtcg caagcagttc cgattggaca attgattgaa cggtactggt aagagcataa
     5341 agtactatcc cgacgccgac catcatgaag acagtcgcaa atgcccggcc tgcgcgcgtc
     5401 ctgggagcga gatccccaaa acccacagtg gtcacagtct gggcggtcac ataaagactg
     5461 tccagtatag tccacccttc tagctcgtga aaaactatcg ttccgaccgt tattgcaact
     5521 aatacagcgg caattccgac cttcgctcga gttcggttgg gacgaaaatt gaacggttcg
     5581 gaggatcttg aacgcatacc gttaaagtgg catcaaagtg tttgcagcgg ccaaatgacg
     5641 gtagctaagg cccactcgac ctgtcaaatc tgaatattga ccgaaccatg acgctaatat
     5701 ggcgtaatat ctagggagtc cgaggcgtca accatcggcc gactgcttat gcctgtacga
     5761 tatccaccaa ctccgccaac gcaatctggc cgaaacgatc caaagaaatc tcagcttata
     5821 tcctattccg attttggtcc aagttttagc aggcgcctga tgagacgact atttagtttg
     5881 agtgttctga ttcctttagt tttcctgggt gcggttgttc ttggagtttt gatctattac
     5941 tggacggtct tttcagctcg catcgatact ctgttgcagg gagaggtcta cacaaagtct
     6001 gccgggattt atgccgcgcc taaacaactt cgagtcaatg agctgatttc gcaggatgaa
     6061 gttgttgcgt ttcttaagcg cgccggatat gtcgaaaaag atcagcaagc cgactcatcg
     6121 cgtggtcgtt atcagtcagg cgtcaatact ctggatatcg agccgagcgg tacttcaact
     6181 gtcgatggtc gacaacagtt tcagaaggtg agagtgcaat tcgcgaagac tggaaaatcc
     6241 atcgcgaatc ttactgatat agacggtcgc gcaaatctgc agc
//
LOCUS       DASSFP010000248         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_215615, whole genome shotgun sequence.
ACCESSION   DASSFP010000248 DASSFP010000000
VERSION     DASSFP010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215615"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFH91_05430"
     CDS             <1..1107
                     /locus_tag="VFH91_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862463.1"
                     /translation="GIAHDLNNVLSPILMAIDMLQLKAADESTEKWLKVLRSNAERGA
                     DMVRQVLSFARGVEGERVAVQPRHLVKEIIKILRETLPKSIEINHKVPNDLWMISADA
                     TQIHQVLMNLCVNARDAMPEGGSIVIRAENQLVDENYAKVNVEAAPGRFVLISVSDTG
                     PGIPQQIQSRIFEPFFTTKEMTKGTGLGLSTALTIVKSHGGFINVYSELHKGTSFSIH
                     LPALDPREKSELASKLKTDLPLGHGELVLVVDDEESIREITKGTLETFGYRVLTASDG
                     TQAVAIYADHPDDVAVVLTDMMMPFMDGPATIRALQRMNPNVRIIAASGLGASQRAAD
                     ASLAGVEIFLDKPYTAEKLLKALAKIISRESVDT"
     gene            1291..1788
                     /locus_tag="VFH91_05435"
     CDS             1291..1788
                     /locus_tag="VFH91_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6862464.1"
                     /translation="MKILLAIDGSPCSLHAVEEVSRRPWPAGSHIRVLTAYEVPVPAT
                     PEGWLLPAEHFDELDRSIKNQATEIVQNAFRTLRSKLPNTLTVEALVIPGSPRAVILD
                     EAKSWDADLILVGSHGYRAWERFLMGSVSQAVVSHAKCSVEVVRAPRSLLVPDNKKSV
                     QKVGM"
     gene            1792..>2092
                     /locus_tag="VFH91_05440"
     CDS             1792..>2092
                     /locus_tag="VFH91_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6862465.1"
                     /translation="MAKVVILGGGFGGVVAAERLAQTLGNEHQITLVSRSRRFTFYPA
                     LVRLAFGKCDRDDVSFDLRSALLDRRANFIESEVARIDPDTQKVIIAHGEVEGQLP"
BASE COUNT          540 a          500 c          550 g          502 t
ORIGIN      
        1 ggtatcgccc atgatttgaa taatgtcttg tcgccaattc ttatggcgat cgacatgttg
       61 caattaaaag ccgccgatga atcaacggag aaatggctga aagtattacg gtcgaacgca
      121 gagcgtggcg ccgatatggt gcgacaggtc ttgtcgtttg cccgaggggt tgaaggcgag
      181 cgagtggccg tgcaacccag gcatctggta aaagagatca tcaagatact tcgtgagacg
      241 ttaccgaagt ctattgaaat caatcacaaa gtaccaaacg atctctggat gatttctgcc
      301 gatgctactc agattcatca ggtattgatg aatctctgtg tgaatgcacg cgatgcgatg
      361 cccgaaggcg ggtcgatcgt catcagggct gaaaatcaat tagtcgatga gaattacgca
      421 aaggtgaatg tggaagctgc cccgggtcgt ttcgtgttga ttagtgtcag cgacactggt
      481 cccggtatac cacagcagat tcaaagtcga atttttgaac ccttttttac cacgaaagaa
      541 atgactaaag gtaccggcct tggtctatca acggctctta cgattgtaaa gagtcatggc
      601 ggtttcataa acgtttatag tgagctacac aagggaacat cgttctccat tcatctgccg
      661 gcgctggacc cacgagaaaa atcagaactg gcgtcgaagc tgaaaactga tctgccactc
      721 ggtcacggag aactggtgct ggttgttgat gacgaggagt cgattcgaga gatcacaaaa
      781 ggcacactgg aaacgttcgg ctatagagtg ctaactgcga gtgacggaac tcaagcagta
      841 gcaatttacg ccgatcatcc agacgacgtt gcggtggtcc taacggatat gatgatgccc
      901 ttcatggatg gtccggcgac aatccgggcg ttgcaacgaa tgaacccaaa tgttcgcata
      961 atcgcggcga gcggactggg tgcaagtcag cgagcggcgg acgcctcgtt agcaggtgtc
     1021 gagattttcc tggacaaacc ctatacagcg gaaaagttac tcaaggctct ggcgaaaata
     1081 atttcacgcg aatcggtgga tacttaggtc ttgcggagaa tttccttccg gctgcgaaat
     1141 accgcgcaat tcctcacaaa cactccttcc tgcatcgtta gattcttcaa ccacttcgtc
     1201 tccaatcttt tggtacacgg cgtgcaaagt gggagttaaa gccaatccat ttttcaagtc
     1261 tcaattaccg attggcaagc gaggtatgca atgaaaattc ttttagcgat agatggctca
     1321 ccgtgcagtt tgcacgcagt tgaagaggtt tctcgccggc cgtggcctgc gggatcacat
     1381 atcagagtgc tcactgccta cgaagtgcct gtacccgcaa ctccagaagg ctggctgctc
     1441 ccagcggaac actttgacga actggatcgt tccattaaaa atcaagctac tgagatcgtc
     1501 cagaatgcct tccgaaccct tagaagcaaa ctgcctaaca cactcaccgt agaagctctt
     1561 gtgatcccgg gatcaccacg cgctgtaatc ctcgatgaag ctaagagttg ggatgccgat
     1621 ttaattctgg tcggatcaca cggataccgc gcttgggagc ggttcctgat gggatcggtt
     1681 tcacaagcag tcgtctctca cgccaaatgc tcggtcgagg tcgtccgagc gcccaggtca
     1741 cttcttgtac ccgacaacaa aaagtccgtc cagaaagtag gtatgtagac gatggcgaaa
     1801 gtggtgatac ttggtggagg tttcggcgga gtagtcgcgg cagaacgttt ggcgcaaaca
     1861 ctcggcaatg agcaccagat tactcttgtc tcgcgcagcc ggagattcac cttttatcca
     1921 gctctggtaa gacttgcttt tggcaaatgc gaccgagatg acgtctcgtt cgacttgcgc
     1981 agtgctctat tagatcgccg ggccaacttc attgaatcgg aagtggcacg tatcgatcct
     2041 gatacacaaa aggtgataat tgctcatgga gaagttgagg gtcagctacc tt
//
LOCUS       DASSFP010000249         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1399875, whole genome shotgun sequence.
ACCESSION   DASSFP010000249 DASSFP010000000
VERSION     DASSFP010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399875"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFH91_05445"
     CDS             complement(<1..1082)
                     /locus_tag="VFH91_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862466.1"
                     /translation="MSEADQAKFESLRRSGLEALYNIDYDKAQKDFKEITRLYPNHPG
                     GPSLLAAALWIKTLYQTRRLQATLYSSASFYSNGDDKVDPNVITEFRALTREARRLSE
                     VRLKQNPKDVEALIFLANTEGLKAAFEEAVERKHFAALKDGNDSVNHHRDVLKLDPTY
                     IDSQLSIGLYEYVIGSLPLPIKMIAGVSGFRGSKKRGLAMIEQVAKEGRWSKDDAKTV
                     LLLLYTREKRFEDILPITRELSAKYPRNYLYRLETADALVAIAAEKRKQKDTAGADQA
                     EKEAFATFDELLRDKNVRETADKAMDLIHFKYGEALFIAGQNERAAKEFLATTKAERA
                     EPGLITMAHLYAARAYDVAGKRDEALG"
     gene            complement(1185..2351)
                     /locus_tag="VFH91_05450"
     CDS             complement(1185..2351)
                     /locus_tag="VFH91_05450"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6862467.1"
                     /translation="MNVTHLECALCGLKHEARTLHNLCRECGKPLLVRYDLARAKESL
                     KKESLLSRRSDLWRYREVLPVENEENIVSLGEGWTPLLPARNLAAKLGLHDLYIKDES
                     QNPTQSFKARGMTTAVSMAKELGAEKLAVPSAGNAAGALAAYAARAGLQSFIFMPRDT
                     PRANVVECEQTGAHVTLMDGLITDCGAEVGRRKEAEGWFDVSTLKEPYRVEGKKTLGY
                     ELAEQLNWELPDLIIYPTGGGTGLIGMWKAFEEMETMGWIGSQRPRMVTVQASGCAPI
                     VRAFEEGKRFADEFPNAATTASGLRVPKAIGDFLILDALRESGGTAVAVTDDELIAAT
                     KVIGAAEGLFCAPEGAACLPALEKLIDSGAVKKNDRVVLFNTGSGVKYMESFDR"
BASE COUNT          634 a          737 c          616 g          714 t
ORIGIN      
        1 ccgagtgctt catctcgttt gccagccaca tcataagctc tcgcagcata aaggtgagcc
       61 atcgtaatca aaccaggctc ggcacgttca gctttggtgg tcgccagaaa ctcttttgca
      121 gctcgttcgt tctgaccagc aataaaaagt gcctcgccat acttgaagtg aatcaggtcc
      181 atcgccttgt cagccgtctc ccgaacattc ttatcgcgta gtaattcatc aaaagttgcg
      241 aacgcttctt tctcagcctg atctgctcct gcagtatcct tctgctttct tttttcggcc
      301 gcgatcgcca ccagtgcgtc tgcagtttcg agcctgtaca gataatttcg cggatacttc
      361 gcactcagct cacgcgtgat tggcaatatg tcctcaaaac gcttttcgcg cgtatataac
      421 agtaataaaa cagtcttggc gtcatctttg gaccagcgac cttctttcgc cacttgttcg
      481 atcattgcca gcccacgttt tttcgaccct cggaaccccg aaacacccgc gatcatcttg
      541 attggtagcg gcagtgaccc aatcacatac tcatacaaac caatgctcag ctgagaatcg
      601 atgtaagtcg ggtccagctt caagacgtcc cgatgatgat tcactgaatc gttgccatct
      661 ttcagtgcag caaagtgctt acgctcaact gcctcttcga aagcggcttt taggccttcg
      721 gtattggcca gaaagatcaa tgcttcgaca tccttggggt tctgcttaag tctgacttcg
      781 gacagtcggc gagcttcccg agttagagcg cggaactcgg tgatgacatt tggatctacc
      841 ttgtcatcgc cgttgctata gaaggatgct gaagaataaa gggtggcttg cagacgtcgc
      901 gtctggtaaa gcgttttgat ccacagagcc gccgccagta aagatggacc acccgggtga
      961 ttgggataaa ggcgcgtaat ctccttaaaa tctttttgag ccttgtcgta atcaatgttg
     1021 tagagcgctt cgagtccgct gcgacgcagt gactcaaact tagcctgatc tgcttcgctc
     1081 aaccaattcg aagtagtctg ttgtgcctgc aacggggagc ttacaaaaaa taggaaacag
     1141 atcagtccaa ataaatactt cacaacatcc ctccaacagt tatttcacct atcgaacgac
     1201 tccatgtatt tcacaccgga accggtgttg aataacacca cccggtcatt cttctttacc
     1261 gctccggaat cgatcaactt ttccagggcc ggtaaacatg cagccccttc cggtgcacag
     1321 aacaaacctt cagcagcgcc aatcactttc gtggcagcta tcaattcatc atcagtaacc
     1381 gcgacggcag taccgcctga ctcccttaat gcatcaagaa taagaaagtc gccaatcgcc
     1441 ttgggtactc ggagtcctga ggcagttgtt gcagcgtttg gaaactcgtc ggcaaaacgc
     1501 ttcccttcct caaatgctct gacaattggc gcgcaacctg aagcctggac agtaaccatt
     1561 cgcggacgtt gagagccaat ccagcccatg gtctccatct cttcgaatgc tttccacatc
     1621 ccgatcaaac cggtcccgcc gccagtagga taaattatta gatccggcag ttcccagtta
     1681 agttgttctg ctaactcgta accgagagtc tttttgcctt ctacgcgata aggttctttc
     1741 agagttgata cgtcaaacca tccttcagct tctttgcgac gtccgacttc agccccgcag
     1801 tcagtaatga gtccatccat caaagtgacg tgcgcgccag tttgttcaca ttcgacgacg
     1861 tttgcgcggg gagtatcgcg cggcataaag atgaaacttt gtagccctgc tcgtgctgca
     1921 taagccgcaa gcgcgcccgc tgcatttccg gcggacggga cagcaagttt ttctgctccg
     1981 agctctttgg ccatcgacac ggctgtcgtc atgccacgcg ctttgaaact ctgcgtggga
     2041 ttttgtgact cgtctttgat gtagaggtca tgtaagccga gtttcgcggc cagatttcgc
     2101 gccggcagca gtggtgtcca gccttcaccg agagagacta tgttttcctc gttctctacg
     2161 ggtaagactt cccggtagcg ccataggtca ctgcgccggc tgagtaatga ttcctttttc
     2221 aaagactcct tggcccgcgc gaggtcgtac cgcacgagca atggttttcc gcattcgcgg
     2281 caaaggttgt gcaacgttcg tgcctcgtgt ttcaggccgc acagcgcaca ttccagatgc
     2341 gtaacattca tcgattttcc tcagtttatg tgtttgattg cgattttgcc tgcccactat
     2401 gctacagaag tctacaggct tgagtgaaga ttaaaaactg caggtgaaat aaaaggctga
     2461 cactcgtaaa cgataccagt gcgaacgcac attacaggaa tcaacacaaa ggaatttttc
     2521 gttatgtttc gtgtgatttc gtggaatcgc tcttcttcca acccggctta ataagcttcg
     2581 aggtgaataa agtaccttta gcgcctccat atgaacgtcc tacttctctt gaaatcgttt
     2641 aactgctcac aaggctgagc aaacaaagcg atagctgact cttgagcctt tggactcgct
     2701 c
//
LOCUS       DASSFP010000250         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1840027, whole genome shotgun sequence.
ACCESSION   DASSFP010000250 DASSFP010000000
VERSION     DASSFP010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840027"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..500)
                     /locus_tag="VFH91_05455"
     CDS             complement(255..500)
                     /locus_tag="VFH91_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862468.1"
                     /translation="MNGLRVYTNVNGFPDALCVYPTFYSRRADGPYYRWRYELNRDKW
                     LASRIGPNDLASAELVQARWYGVPDALKSSLSEHYLE"
     gene            complement(497..799)
                     /gene="rpoZ"
                     /locus_tag="VFH91_05460"
     CDS             complement(497..799)
                     /gene="rpoZ"
                     /locus_tag="VFH91_05460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET6862469.1"
                     /translation="MKETSEKDWLGVMSRFQLVILASLRSKQLLKGAHPRIAADPRKR
                     RNTSIALEELKRGLVPFKGRSVALAENGETPDTAMISLSLLGQGAEPKLVEAELTR"
     gene            complement(889..1161)
                     /locus_tag="VFH91_05465"
     CDS             complement(889..1161)
                     /locus_tag="VFH91_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862470.1"
                     /translation="MAQEITMNYVRRNKKNVAGSIVVVMVLTGIAVWQFYRFVTFADT
                     SGIPNSQAGLGHLLWAIVMTMCACVIGFFVSSVFLRRDNDEVIHIT"
     gene            complement(1229..1537)
                     /locus_tag="VFH91_05470"
     CDS             complement(1229..1537)
                     /locus_tag="VFH91_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862471.1"
                     /translation="MFGRQVTMKLKPGSGSEFIRINQTLVKTILREQQGFLSETTHIN
                     PEHSEVTANSLWNTKEDEVRYDETAYLEIVKALADVVEGTPMVKTFELAQTYFHKIAA
                     "
     gene            complement(1679..2785)
                     /locus_tag="VFH91_05475"
     CDS             complement(1679..2785)
                     /locus_tag="VFH91_05475"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6862472.1"
                     /translation="MAETNNWEPSAGVLTFARDHAFQKELRRRVEEYFRVSGRRQRDC
                     PEMYLKTVVIMAFFAGCYSLLVFAAQTWWQAVPLALLTGLSMAVIGFNIEHDGGHRSY
                     SDHEWVNKLMAMTLDLIGGSSYVWHWKHAVVHHTYVNITGHDADIDVGIFGRLSPHQE
                     RLGFHRWQHFYLWPLYGLNVIKWHLYDDFRDVILGRIGSTRIRRPGFWDFLGFIGGKA
                     LFFTLALIVPSFFHSFWLVLLFYVLTVSVAGVVVSVVFQLAHCVEGADFPMPRADNYS
                     MENAWAVHQVETTVNFARRSRAESWLLGGLNFQIEHHLFPRICHVNYPALSHLVEQTC
                     REFGITYREHHSFRAGLVSHFRWLRRMGAPVAEA"
     gene            complement(3349..4518)
                     /locus_tag="VFH91_05480"
     CDS             complement(3349..4518)
                     /locus_tag="VFH91_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6862473.1"
                     /translation="MKTERTKRQPDIRTITRLVPPSGNLVQGQSELAINPRLADAASK
                     IIHASQNHYSPAEGAADLREAVAKKIARFNGIELDPKATPLQLLITPGATGGLIAIAH
                     TYLKGSSALVFEPYYPYHRRILNELDGRTELVTLRGENLELDKDELRTRCRELKGRSD
                     YPLRAIIACSPANPTGKVFTAAEFEIIADACKEFDLLCIADEVYEHYVVGDRTHLCIA
                     NLDGMFERTITVNSFSKSWNISGWRLGYVYGPSQLIAPLNNTNNVFYVCSPTPLQRAL
                     GEVLMSDPNYYSELRKLFEGKRKVATEALEQVGFKIYDSNSAFYLWARSPEGYRDAIE
                     LNEILIANGVAGVPGSAFADGDEFDNFMRFCIAREDDVLRGALNKIQETVSIVKS"
     gene            complement(4538..>4892)
                     /locus_tag="VFH91_05485"
     CDS             complement(4538..>4892)
                     /locus_tag="VFH91_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862474.1"
                     /translation="LWQPWDHECHLMGTHNPERGGARRAQTVAIPSGLRQKQSPAFIP
                     GLPKLNPGLKLANTFGVPLQLLAAVWYYSSPLTTWRELKTDLRRIGQNTIEHTHLRFH
                     IIWFGKYYVSSTSIF"
BASE COUNT         1240 a         1257 c         1168 g         1227 t
ORIGIN      
        1 tcactccgct cgagtgacat gtttgtagac ttggacgtac tccttctact ccactccgtt
       61 cgaagttcta agaaggagcg actaggactg tacgcttcaa gatctctacc gctccttctt
      121 agaacttcga acggagtggg actgtctctg ggaagttgtc ggctataaac atgtcaccca
      181 acgaggtgaa atccaatgct cttcccattt ggtaacaaat cggctcttgc tttttccagg
      241 gagaatcttc aagtctactc gaggtaatgt tcactcaaac tcgacttcaa cgcgtccggt
      301 acaccgtacc aacgcgcctg gaccagctca gcggaggcga gatcattggg tccaatccgc
      361 gaggccagcc atttatcgcg gttcagttca tagcgccaac ggtaatacgg tccgtcagct
      421 cgccggctat aaaaggtggg atagacgcat aacgcatcgg ggaaaccgtt cacgttggtg
      481 tacactcgaa gaccgttcat cgtgttagtt cagcttccac tagtttcggt tcggcgcctt
      541 gccctaacaa actaaggctt atcatcgcag tatcgggagt ctctccattt tccgccagag
      601 ctacagatct tcccttgaaa ggtacaaggc cccgtttcaa ttcctccagg gcaatgcttg
      661 tattcctccg ttttcgcgga tcagcggcaa tgcggggatg cgcacctttc aaaagctgtt
      721 tgcttctgag gctcgcgagg ataaccaact gaaatcgcga catcacgccg agccaatctt
      781 tttcgcttgt ttctttcatc tcgttaaata gggcaacatc aaccttgcgg taaccgattt
      841 gaattcgacg ggggctattc cagctctcct gagtccgttt gcggcgtttt acgttatgtg
      901 tatgacctca tcgttgtcac gacggagaaa aactgacgag acaaagaacc cgatgacgca
      961 ggcacacatc gtcataacta tggcccataa gagatggccc aaaccggcct gggagttcgg
     1021 aataccgctt gtatctgcaa atgtaacgaa tcgataaaat tgccaaacag cgataccagt
     1081 caaaaccatt actacgacta tcgaacccgc aacattcttt ttattccgtc tcacataatt
     1141 catcgtaatc tcctgagcca ttcccgttaa ctgctttatc tgccgagcgt gctattccga
     1201 tgtcgtgccg cattaactcg tttgagtctc aagccgctat cttgtgaaag taagtctggg
     1261 caagctcaaa ggtttttacc atcggggttc cttccacaac gtctgccaat gccttgacta
     1321 tttctaagta agcagtttcg tcgtacctta cctcatcctc tttggtattc cacaggctgt
     1381 tggcagttac ctcggaatgt tcgggattga tatgcgttgt ctcgctcaag aaaccttgct
     1441 gctctcgcag gatggtctta acgagcgtct gatttattcg aataaactcc gagccagaac
     1501 ctggcttcag cttcatagtg acttgcctgc caaacatact ttgctcctga ttttcggagt
     1561 gctgttgact tcaggtggtc tggatgattc cagaagagct gagaagggag tgagcgaaac
     1621 tcgattcaca tcaaccttac gctcgcccta tctaagtgtc aatgacagga gtatttaact
     1681 aggcttcggc tacgggggcg cccattcggc gcaaccaacg aaagtgcgag acaaggcctg
     1741 ctcggaacga atggtgttct cgataggtta tgccaaattc gcggcaggtc tgttccacca
     1801 gatgagaaag tgcggggtaa ttgacatggc aaatacgcgg gaaaaggtga tgttcgattt
     1861 gaaaattcaa gccgccaaga agccagcttt ctgctcgact gcgtctggca aaattaactg
     1921 tcgtctccac ctgatggacc gcccaggcat tttccatcga atagttatcg gcgcgaggca
     1981 ttggaaaatc agcgccttcc acgcaatgtg ctagttgaaa gaccacacta acaactacgc
     2041 cagcgacgct cacggtaagc acatagaaaa gtaggaccag ccaaaaggaa tgaaagaacg
     2101 aggggacgat gagggccaaa gtaaagaaca gcgccttgcc gccgataaaa cccagaaagt
     2161 cccagaatcc aggccggcga attcgggtcg aaccaatcct tccgaggatt acgtcacgaa
     2221 aatcatcata gaggtgccat ttaataacat tcagcccgta gagcggccat agataaaaat
     2281 gttgccagcg atgaaaccct aatctctcct ggtgcggact caggcgacca aaaatcccga
     2341 cgtcaatatc agcatcatga cctgtgatgt tcacataggt gtgatggacc accgcatgtt
     2401 tccaatgcca gacgtaagaa ctgccgccga ttaagtccag cgtcatggcc ataagtttat
     2461 taacccactc gtggtctgag taagaacgat gtccaccatc atgttcaatg ttgaaaccta
     2521 taacggccat tgacaaacca gtcaggagtg cgagcggtac agcctgccac catgtttggg
     2581 ccgcaaagac gaggagcgaa taacaaccgg caaaaaacgc catgatgacg accgtcttca
     2641 aatacatttc gggacagtct cgttggcgcc gtccgctaac gcgaaagtac tcttcaactc
     2701 tgcgccttag ttccttttga aaggcgtgat cgcgtgcgaa ggttaagacc cctgcagaag
     2761 gctcccagtt atttgtttct gccatttact tttcaaactc ctaacagctt gttgctctca
     2821 tcgattacta ccttaaccac tgtacagagg acgcgtcccg atttctcctg gaaatttgaa
     2881 atcaggcgag cctttacaaa aacattacac agcgaagcga tttagaggag ggttgtgctt
     2941 atgacgcttg atgatcttgt tggagttcct taaagagcta cacaacgcaa gacgcagaga
     3001 acgcggagat cacgcagaga atcatgcagt tgggaagggg gcaaagcggc caacatgagc
     3061 acactacaca gtcgaacaca gcgtcgcccc cgcgctttag tcttccaaac agagtttcgt
     3121 cattccagtg aagttgtgtt gagggtaaag agcgggggcg acgcttggtt cgagttgggt
     3181 ctagtggctt ttgctggtcg ctttgccccc ttcccaactg catgattctg gtatgtcgca
     3241 gagccccttc ccaactgtgt gattctggct gttgctcagg attgccgatt gcccgcttct
     3301 caaactgcgt gagtctggaa cagtccaggt aaaccagaat gctgaaacct acgacttaac
     3361 tatcgataca gtctcttgaa tcttattaag agcgcctctc aagacatcgt cttcacgagc
     3421 gatgcagaag cgcatgaaat tatcgaattc atcgccatcg gcaaacgcgc ttcccggaac
     3481 tccagcgaca ccattcgcaa tcaagatctc gttcaactct atcgcatcac gatagccttc
     3541 cggacttctg gcccacaaat agaatgctga gtttgagtcg tagatcttaa agccaacctg
     3601 ctccaatgcc tctgtggcaa ccttacgctt accttcaaat agcttcctta actcgctgta
     3661 gtagtttgga tccgacatca gcacttcgcc caatgctcgt tgtagaggtg ttggcgagca
     3721 aacatagaag acattattcg tgttgttcag aggcgctatc aattgactcg gtccatatac
     3781 ataaccaagc cgccaacccg agatgttcca cgacttggaa aatgagttga ccgttatggt
     3841 cctctcaaac atcccatcaa ggttagctat gcataggtgt gtacgatcgc ctactacata
     3901 atgctcataa acctcgtcag cgatacacaa caagtcgaac tctttacaag cgtccgcgat
     3961 tatctcgaac tccgcggcgg tgaagacctt accggttgga ttggccggac tgcaggcaat
     4021 gattgctcgc agcggataat ctgatcgacc tttgagttcg cggcaacgag tacgtaattc
     4081 atccttgtcg agctcgaggt tttcacctcg cagcgttacc aattcagtac ggccatctaa
     4141 ttcgttgaga attcgacggt gataggggta gtacggttca aagacgagcg ctgaacttcc
     4201 cttcaaatat gtgtgcgcga ttgcaattaa accgccggtc gcaccaggtg taattagcag
     4261 ctgcagagga gtagcttttg gatcgagttc gataccattg aatcgcgcaa tctttttcgc
     4321 taccgcctcg cgcaaatcag cagcgccttc ggcaggactg taatgattct gactcgcgtg
     4381 aataattttg ctcgcggcat cagcgagacg aggattgata gctagttctg attgcccctg
     4441 gaccaaattg ccagacggcg ggaccaggcg cgtgattgtt ctgatgtcag gttgcctttt
     4501 cgttcgttca gttttcataa cagaagccgt catagtctca gaaaatggaa gtcgaactca
     4561 catagtattt gccgaaccaa attatatgaa aacgaagatg cgtatgttca attgtgtttt
     4621 gaccaattct gcgcaagtcc gttttcaact ctctccatgt agttagcggt gatgaatagt
     4681 accagactgc cgcaagaagc tgcaggggta caccgaaggt gttggccaat ttcagcccag
     4741 ggttgagctt tggcaaccct gggataaagg cgggtgattg tttttgtcgc aatcctgaag
     4801 ggattgcgac ggtttgcgcg cggcgggcgc cacccctttc agggttgtgc gttcccatca
     4861 aatgacactc gtgatcccag ggttgccaaa gc
//
LOCUS       DASSFP010000251         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1258495, whole genome shotgun sequence.
ACCESSION   DASSFP010000251 DASSFP010000000
VERSION     DASSFP010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258495"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFH91_05490"
     CDS             <1..284
                     /locus_tag="VFH91_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862475.1"
                     /translation="RYNIVSERDLKLAATALETYLKSEYWSVSDHPTKQELLHPTKKL
                     SLNCIELNTTQPQQSSNICSLYLDSSANICRCSTRETIFSPEGDNYEFI"
     gene            271..1032
                     /locus_tag="VFH91_05495"
     CDS             271..1032
                     /locus_tag="VFH91_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862476.1"
                     /translation="MSSFKNYGLNTTNIVTKMLNQSINRLPLIKIKNFILISLLVATL
                     IPVPLQSAAQSPHSGGSPADTFSILLSGPYKAVPEKPIGDCPDLGLFQANICDGSYSK
                     TKIFPVSGLPKEASGQANSGEGPRSGERQAENAIGTFYVQFVGIHAVYDLPGGALTMF
                     FTANNLVPAPDGQGGTYFVGTVDLDIVEATGIFESFVGGHNKMVDILHQLADGTFVEH
                     CFCVISRPQQSAAITPHASQGETGDRRFDREDATV"
     gene            1168..1653
                     /locus_tag="VFH91_05500"
     CDS             1168..1653
                     /locus_tag="VFH91_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862477.1"
                     /translation="MQWKLASRMSMVTIPIMIVCGLLVPASSMGPTVRADDEDTKVCT
                     NHTLRGDYGSASEGVLLNVPGLPPEAQFRGLTMTSFDGKGNLTWVEHTVINGTPLQPG
                     WTPASGTYAVNPDCTGTAVVNTPNSPVPLHLALVVVRQGKEIHAVLDSDAISTVFNKV
                     E"
     gene            1853..1981
                     /locus_tag="VFH91_05505"
     CDS             1853..1981
                     /locus_tag="VFH91_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862478.1"
                     /translation="MKIAPVVKSVFYRIASDIRDLVLNENLQNEQNSRSPLGTGLK"
     gene            2066..2545
                     /locus_tag="VFH91_05510"
     CDS             2066..2545
                     /locus_tag="VFH91_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6862479.1"
                     /translation="MKTIISGTLLFGTVIAVVLITVGMPIWSQQGKWASETAPAAKYM
                     IDLERKWAEAGCTHQLAGDTFADDFQGTAPDGSRYDKAQALQTDSSVTERECHLDDAK
                     VRLFGDNTAIVYGSERAMLKPQGQTESMRCLVWTDTWLKRNEKWQIVAAQDTAVPCK"
     gene            complement(2590..2970)
                     /locus_tag="VFH91_05515"
     CDS             complement(2590..2970)
                     /locus_tag="VFH91_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862480.1"
                     /translation="MSNELAVQIAEIQKRLDALEARRTPGPRGPAGDITAAVENAEKA
                     AKAIVMERLDSYIQRTETIARRVQQQVDSANQNIRNEFQSIVKSAAVKALRDALENEV
                     ALLVLKILVEYQVADIAGDPIKID"
     gene            complement(3090..3539)
                     /locus_tag="VFH91_05520"
     CDS             complement(3090..3539)
                     /locus_tag="VFH91_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862481.1"
                     /translation="MRLRDLIHQTLIKSHGSKYWEKIVPEDIRNEAEKRISNELRKQR
                     ELSPAHFSDPRKKLDYCNVLDYSKLILNNSNWAYFEPFFVRRLDVERHLEAFSDFRNS
                     LMHNRELTEISKRAGELAIVWFETTLPGTAGQEAIEEDSEEPSEDHE"
     gene            complement(3503..>4886)
                     /locus_tag="VFH91_05525"
     CDS             complement(3503..>4886)
                     /locus_tag="VFH91_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HET6862482.1"
                     /translation="GQQRITSLYVTLMGLTARGTDYSRICFDAKDQKFTFRDPDNKRY
                     VSVCDIWGDAALDILDSLEKPFTAAYKRCWKILQTYPISTVIVAVKDLPAVCKIFQRI
                     NQSGKRLDRFDLISSMTFSTDFDLREKFTQDILTFLKENAFGEISPATVTQLMALLKN
                     GACTERAEYSLTAQEIKELWQTVIEGILLAADTLRKNFGVKNRQYLPYDALLTLLAYF
                     FAKYRNRSLTGEQLEWVSKWFWRASFSLHYGSGGPTKMGQDAELFDSLQKGEQPPFKP
                     SMNLTTETLIGTKMTRSGSAIRNAFLCLLAQRGPLHLVNNGPLDLINGSVSDFTSAEK
                     HHIFPQAFLRRSGVAPAEVHDLPNFCFLPAELNKRISDTRPSVYFPELATENKRLSEA
                     LGTHLIPLGEDSGLQDDNYLQFLKSRAEMILQEIERLSGLSVAPLKTKDSMLLSRSRC
                     AFATSYTRHS"
BASE COUNT         1157 a         1265 c         1263 g         1201 t
ORIGIN      
        1 agcgctacaa catcgtttct gaacgtgacc tgaagctcgc agccacggcg ctcgaaacgt
       61 acctcaaatc tgagtattgg tcagtttccg atcaccccac taaacaggag ttactccacc
      121 cgaccaagaa attgtccctc aattgtatag aattgaacac tacgcaacct caacagtcct
      181 ctaacatctg ctcactctat ctggactcgt ccgcaaacat ttgccgctgt tcgacgagag
      241 agaccatttt ctcgcctgaa ggagacaact atgagttcat ttaaaaatta tgggctgaac
      301 accacaaata tcgtgactaa aatgttgaac caatcaatca accgcctgcc tctgatcaaa
      361 atcaaaaact tcatcctcat ttctttgctc gtggcgacgc tgattccagt tccattgcag
      421 tccgctgcgc aatcaccgca ttcaggcggg agccccgccg acactttctc tatcctgcta
      481 tccggtccgt ataaagcagt tccggaaaag cctatagggg attgccccga ccttggattg
      541 ttccaggcaa acatctgtga cggttcctac agcaaaacaa agatctttcc agttagcggg
      601 cttccgaagg aagcgagcgg gcaggccaac tctggcgaag gcccgcgcag tggcgagcgt
      661 caagcggaga acgcgatcgg caccttttat gtacagttcg tcggcattca cgctgtttac
      721 gacctcccgg ggggcgcgct gacgatgttt ttcacggcca acaacctcgt acctgctcca
      781 gatgggcaag gcggaacgta ctttgtggga acagtggatt tagacatagt ggaggcgact
      841 ggaatcttcg agtcttttgt cggtggtcac aacaagatgg tggatatact gcatcagttg
      901 gccgacggca ctttcgttga acactgtttc tgcgtcatca gccgcccaca gcagtctgcg
      961 gcaatcacgc ctcacgcttc tcaaggtgaa acaggggata gacgattcga ccgcgaggac
     1021 gcaacggttt gaggggctgt cgctgaatcc agagcaacaa ggtctgcttc ggatagaggg
     1081 tccgtctcct gtcaggcact agaccattga tgacctgact gaattagatc aaacattttg
     1141 gcggttgagt actaaaagga ggattcgatg caatggaaat tggcttcgag aatgtcgatg
     1201 gtgacgattc ccataatgat tgtttgcggt ttactggtcc cggcttcctc aatgggtccc
     1261 acagtgcggg cagacgacga ggacacaaag gtgtgcacga atcataccct tcgcggcgat
     1321 tacggctcag catccgaagg cgttctcctt aacgtcccag gccttccgcc cgaggcgcaa
     1381 ttccgcggat taaccatgac tagtttcgat ggcaaaggta atctgacgtg ggtagagcat
     1441 acagtcatca acggcacgcc actacagcca ggttggacgc cggcgagcgg gacgtatgcc
     1501 gtcaatcccg actgcactgg aacagctgtg gtcaatactc ccaatagtcc tgttccgctg
     1561 cacctggccc tggtcgtggt tagacagggc aaggaaattc acgccgtgct cgactcagat
     1621 gcgatctcca ccgtattcaa caaagttgag taatcgttgc gataagtagg ttgtgacgat
     1681 ctgttctatc gtttgcacgg ccgtggcgtt cctcagaacg agcacgtccc ggcgcaactc
     1741 ccattacgct cgtcgccgaa atgccggacc cacatcgcca cttgttacgc tcccgaccga
     1801 cggttcccca gtcctgattc gcgcttactg gacaagtccc taggcgcgac cgatgaagat
     1861 cgcgcccgtc gttaagtcag ttttttatcg gatcgccagc gatatccgcg acctggtact
     1921 caacgagaat cttcagaacg agcagaattc caggagtccg ctcggtacag ggttgaagtg
     1981 aacgacaagg ggcattaacc tcgcaattga cagttaggtg tatatacgat gctaattgca
     2041 ctggcatcgc gaagggagga gcgcgatgaa gactatcatt tcggggactc ttctttttgg
     2101 taccgtgatt gcggtcgtgc taattacggt gggtatgcct atctggtcgc aacagggaaa
     2161 atgggcttcg gaaactgctc ctgccgcgaa gtacatgatc gaccttgagc gaaaatgggc
     2221 cgaggcaggt tgcacccacc aactagccgg agacacgttt gccgatgact tccaaggtac
     2281 cgcacccgat ggttcgcgtt atgacaaggc tcaagcactt caaaccgata gctctgtaac
     2341 cgaacgtgag tgtcatttgg acgacgcgaa ggtgcgcctg tttggcgaca acacagcgat
     2401 tgtttatggc agcgaacgag ccatgctcaa gccgcaaggg cagaccgaga gcatgcgttg
     2461 tctcgtttgg acggacactt ggctgaagcg aaatgagaag tggcagatcg ttgccgcaca
     2521 ggatacagct gtgccctgta aatgacgcat tgaaataaaa gcgcgaccaa tgaagatcgc
     2581 gcccgttgtt cagtcgattt tgattggatc gcctgcgatg tccgcaacct ggtactcaac
     2641 gagaatcttc agaacgagca aagccacttc gttctcaaga gcatcgcgca atgccttaac
     2701 agctgcgctc ttgacgatgc tctgaaactc gttacggata ttctggttag cgctgtcgac
     2761 ttgctgttga actcggcgag ctatcgtttc agttcgctgg atgtacgagt cgagccgctc
     2821 cattacgatt gccttggccg ctttctcagc attctccacg gccgccgtaa tgtctccggc
     2881 cgggccacgg ggtcccggag tccttcgtgc ctccaaagcg tctaaacgct tctggatctc
     2941 tgcaatctgt actgctagtt cgttactcat gagtttctcc acacgcatct tcgaatgatg
     3001 cgcgatactt cgacaacgtc tgaacacgga cacgaaggca ggtaccaaga ctcgggaacg
     3061 gactgtcagg ttgccattgg gaaatggcgt tactcatggt cctcggaagg ttcctcagaa
     3121 tcttcttcga tcgcttcctg cccggccgtg ccagggaggg ttgtttcaaa ccagacaatc
     3181 gccaattcgc cagccctctt cgagatttcg gtcagctcgc gattgtgcat taggctgttt
     3241 cggaaatcag aaaacgcttc taagtgtcgc tccacatcaa gtcggcgaac gaagaacggt
     3301 tcaaaatatg cccagttgct gttgttcaga atcagttttg agtagtcgag cacattgcag
     3361 taatccagtt tcttgcgcgg atcagagaaa tgtgcgggac tgagttcacg ttgctttcgc
     3421 aactcattgc taatccgctt ttcggcttcg ttacgaatat cttctggaac tatcttttcc
     3481 caatacttcg acccatggga ttttatgagt gtctggtgta tgaggtcgcg aaggcgcatc
     3541 tcgatcgact taatagcatt gagtctttgg ttttcagggg ggctacagac agcccagaga
     3601 ggcgttcgat ttcctgcaaa atcatttcgg ctcgtgattt tagaaattgt agatagttgt
     3661 catcctgcag accgctatcc tcgccgagtg gaataagatg ggtaccaagg gcctcgctca
     3721 gccgcttatt ttccgttgcc aattcaggga agtacacaga gggccgggtg tcacttatac
     3781 gtttattaag ttcggcgggc aagaaacaaa aattcggcaa atcgtgcact tccgccggcg
     3841 caacgccgga acgacgcaga aatgcttgag gaaagatgtg atgtttttcc gcactagtaa
     3901 aatctgagac gctcccgttt atcaagtcga gtggcccgtt gttgaccagg tgcagtggtc
     3961 cacgctgcgc caaaaggcaa agaaaggcgt tccgtattgc agatccggac cgcgtcattt
     4021 tggttccgat gagtgtttct gtcgttagat tcatcgacgg cttaaacggt ggttgctctc
     4081 ctttttgaag actatcgaag agttcagcgt cctgccccat cttggtcggt ccaccagaac
     4141 cataatgcag tgaaaaagat gcccgccaga accatttgct cacccactca agttgttccc
     4201 ctgtgagcga cctgttccga tacttcgcga agaaataagc aaggagggtg agtagtgcat
     4261 cgtacggtag atattgacgg ttcttgaccc cgaagttctt ccgaagtgtg tcggccgcga
     4321 gcaggatgcc ttcgatgact gtctgccaca attccttaat ctcctgagca gtcaagctgt
     4381 attccgctct ctcagtgcaa gctccgttct tcaacagcgc catgagttga gtcactgtgg
     4441 ctggcgagat ttcaccgaaa gcattttctt tgagaaacgt caagatatcc tgggtaaatt
     4501 tttctcgaag atcgaaatcc gtcgagaaag tcattgagga gattaggtcg aagcggtcca
     4561 gccgtttacc cgactggtta attcgttgga aaattttgca tacggctggt aggtctttga
     4621 ccgcgactat aactgtagag atgggataag tctgaagaat cttccaacag cgcttatacg
     4681 cggccgtgaa aggtttttct aaactgtcca ggatatctag tgctgcgtct ccccagatat
     4741 cgcagacact gacgtatcgc ttgttatcgg gatctcgaaa ggtaaatttc tggtctttgg
     4801 cgtcgaagca aatacggctg tagtcggtcc ctcgcgccgt gagtcccatc aatgttacgt
     4861 acagagaagt gatccgttgt tgccca
//
LOCUS       DASSFP010000252         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_661387, whole genome shotgun sequence.
ACCESSION   DASSFP010000252 DASSFP010000000
VERSION     DASSFP010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661387"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFH91_05530"
     CDS             complement(<1..185)
                     /locus_tag="VFH91_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862483.1"
                     /translation="MTRSVKHIASILLLVVIVGVSVSAQTTTDPQKTRQRTAAGQDDK
                     TKKPVDRLEPNETPRTP"
     gene            complement(212..1600)
                     /locus_tag="VFH91_05535"
     CDS             complement(212..1600)
                     /locus_tag="VFH91_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HET6862484.1"
                     /translation="MRVLRLNNRTVLLIFAEAVLIFGAIIGAVYLRTGFDGAPYELIT
                     RHGYWKAGVAAAFCLAAFYLFDLYDFVVIHDRRELTLRLIQALGLAWIALALAFYAYP
                     GLMLGRGIAVIALPLALGLMVAWRVSIHWLLGHPDFGEKILIVGSGNLAVEVAREVLN
                     RPDAGYRIVGFVGNDAELLGKSLINPRVIGLTEQLGEIVQREGIDRIVVAMGERRGQL
                     PTDKLLQLSLAGHVTIEEGASFYERVTGRVSLAMIRPSWLIFTGRGRQAKGSLILRNL
                     VHRLVALIGAILSLPIVIVTAILIKLDSRGPVFYRQERVGKNGRPFVLTKFRSMRVDA
                     EAGGPVWATKGDDRTTRVGKIIRKIRVDEIPQFWSILRGEMSFVGPRPERPHFVKQLA
                     DEIPYFEQRHLIAPGLTGWAQIKYPYGASIEDARQKLQYDLFYIKNQGLLLDAVIMFE
                     TIKIILFGRGAQ"
     gene            complement(1602..2672)
                     /locus_tag="VFH91_05540"
     CDS             complement(1602..2672)
                     /locus_tag="VFH91_05540"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET6862485.1"
                     /translation="MGRVFDAIKRQSASPNGTGKKSENESHRNHDQKPVALPNAKEVE
                     EQIFSGSSIISIASETVQSSASTAHTADVPDGSALPGGFASRDAGATLDAAGAARVGG
                     TVCYDISSARVEPHLIAVSQPRSAYAEQFRALRTRILQAGERMQMRAFVVTSAGIAEG
                     KTLTALNLAWLLAQTEGIRALVIDSDLRRPCATEYLGIEAPVGLSEVLGGQLSIEDAI
                     VRLDPAGLYLLPGGQPREDVAELLSGPTYARLLTDVRRMFDYIIIDAPPLGIFTDANV
                     LMNRADAALLVVRAGKTRYAAVDRLLEQIPREKLMGVVLNRVEGQPDGTSYYYQQRYY
                     YQQRGPKRVVNDPEDKREVAIG"
     gene            complement(2716..3672)
                     /locus_tag="VFH91_05545"
     CDS             complement(2716..3672)
                     /locus_tag="VFH91_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6862486.1"
                     /translation="MYAEFYGLKELPFALTPDPRFIYFTPSHTEVMANLHYGIESGKG
                     LVVVTGEVGTGKTTILRWMMQRLDRTVLVAYIFNPRLSVPEFYQHVATLLDLQKWETK
                     SELLLELGRALESRHSRGLRTVLIVDEAQGLSPHVLEEIRLLSNYESNTAKQLQIVLT
                     GQPELREVLNNPDLRQLKQRVALRCVIKPLPNVEETDRYITSRLLVAGGERSGIFSPA
                     AIDYIFRCSDGIPRNINNLCDNALLAGYAAGETTISRAIIEEVAETFDMLPRSDRGMQ
                     RGEEREAPASIFSSATRAELWAAGTPTADHETENVTIVPEPS"
     gene            complement(3716..>3919)
                     /locus_tag="VFH91_05550"
     CDS             complement(3716..>3919)
                     /locus_tag="VFH91_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862487.1"
                     /translation="ARHYTGLPVLIAVPELLTPQEARAVPRRRRLMLAAGFAVTLVSI
                     PLLALALKLTHVFELLSQSSGRA"
BASE COUNT          981 a         1094 c          931 g          913 t
ORIGIN      
        1 cttggggtgc gtggtgtttc gttaggctca aggcgatcta ccggtttttt ggttttgtcg
       61 tcttgtcctg ccgctgttcg ttgtcgagtc ttttgcggat cggtcgttgt ctgggcggaa
      121 acagatacgc ccacgatcac aacgagcaaa agaatcgagg caatgtgttt tacacttctg
      181 gtcatcttcg ctacctccaa ggtcgctcga atcactgagc tcctcgtcca aacagaatta
      241 tcttgatggt ttcaaacata attaccgcgt caagaagcaa cccctggttt ttgatgtaaa
      301 agagatcgta ttgcagcttt tgccgtgcat cttcgataga agcgccgtac ggatatttga
      361 tctgcgccca accggtaagt ccgggcgcaa tcaggtgtcg ctgctcgaaa tagggaatct
      421 cgtcagcaag ctgttttacg aaatgtggac gctcaggtcg cggtccaaca aagctcattt
      481 cgccccgcag gatgctccaa aactggggaa tttcgtccac tcgaattttt cgaatgatct
      541 tacccactct ggtcgtacgg tcatcccctt tggtggccca aacaggccca ccagcctcgg
      601 catcaaccct cattgacctg aactttgtaa gaacaaacgg tcgaccattt ttcccaactc
      661 gctcctgtcg gtaaaaaaca ggaccgcgtg aatcaagttt gataagaatg gcagttacga
      721 tgactatcgg cagtgaaagt attgcgccga tgagcgccac cagccgatgg accaaatttc
      781 gaagaattaa cgatcccttt gcctggcggc cccgtcccgt gaagatcaac cacgaaggac
      841 ggatcatagc cagcgagacg cgtccggtga cccgttcata aaacgatgca ccttcttcga
      901 tggtgacgtg cccggccaaa cttagctgca acagtttgtc ggttggcaat tgtcctcgcc
      961 gctctcccat ggcaacaaca atgcggtcga ttccttcccg ctgaacgatc tctcccagct
     1021 gttctgtaag cccgattacc ctgggattta tcaaactctt ccccaggagt tctgcgtcat
     1081 taccaacaaa accgacaatg cgataaccgg catcgggacg attaagtact tcgcgtgcaa
     1141 cttctactgc gagatttccc gagccaacga tcaaaatctt ttctccgaaa tccggatggc
     1201 ctaacaacca atggattgag acacgccagg ccaccatcag ccctaacgcc agcggcaacg
     1261 caatgacagc gataccacga cccaacatca aaccggggta ggcgtaaaaa gcaagggcca
     1321 gcgcaatcca tgctagaccc agagcctgaa tgagtctgag agttaattcc cttcgatcgt
     1381 ggatgaccac aaagtcgtac aaatcgaaaa gataaaatgc ggctaaacag aacgctgccg
     1441 ccacgccggc tttccagtaa ccgtgacgag ttatcaattc gtaaggcgca ccatcaaagc
     1501 cggtgcgtag ataaacagcg ccgatgatcg caccaaagat aagaacagct tcggcgaata
     1561 tcagtagaac tgttcgatta ttgagacgta ggactctcaa tttatccgat tgctacctcc
     1621 cgcttgtcct ctgggtcatt gacaactctt tttggaccac gctgttggta atagtaacgc
     1681 tgctgatagt agtaagaagt gccgtccggc tgtccctcta cgcgatttag aactacaccc
     1741 attaattttt cgcggggaat ctgctcaagc aacctgtcca cggctgcata acgagtctta
     1801 ccggctctca ccacaagcaa tgcggcatca gctcggttca tcaatacgtt tgcgtctgta
     1861 aagattccga gcggtggcgc atcgatgatt atgtaatcga acattcttct tacatcagta
     1921 agcagccgag cataagtcgg accagacaac aattccgcaa catcttctct cggttgaccg
     1981 ccaggcaata aatacaaacc tgccggatcc agacggacta tcgcgtcctc aatgctaagt
     2041 tgtccgccca atacttctga taatccaacc ggcgcttcaa tacccagata ttccgtggcg
     2101 caaggtcgtc tcagatcgct atcgataact aaggcacgaa ttccttcggt ttgggctagt
     2161 aaccaggcca ggttgagagc cgttaacgtt ttaccttcag ctattcccgc actggtgaca
     2221 acaaacgcac gcatttgcat tcgttcaccg gcctgaagaa ttctcgtacg caaagcgcga
     2281 aattgttcag cgtatgccga gcgaggctga ctcaccgcaa tcaggtgagg ctcgacgcgc
     2341 gccgaagaaa tgtcataaca gacagttccc ccaacgcgcg ccgccccggc agcgtccaaa
     2401 gttgccccag catcacgcga agcaaatccg ccggggagtg ccgacccgtc gggtacatcc
     2461 gcagtgtgcg cagtggacgc tgaactctgc accgtttcgc ttgcaatgga gataatcgat
     2521 gaaccactaa aaatctgttc ttcgacttcc tttgcattgg gcaacgcgac cggcttctgg
     2581 tcgtgattac gatgcgattc attctctgat tttttaccag ttccattagg actggcgctt
     2641 tggcgtttga tagcgtcaaa aactcttccc atgtaatcac ctctcaggac ttctgaagtt
     2701 tcagaagact cacaattagc taggctccgg aacgatcgtc acattctcag tttcatggtc
     2761 ggccgtaggt gttcctgccg cccacaactc tgctcgtgta gcagacgaga aaatgctggc
     2821 cggtgcctca cgctcttcgc ctctctgcat tccgcgatcg gaacgaggaa gcatgtcaaa
     2881 ggtttcagca acttcttcaa taatcgctct gctgatagtt gtttcaccgg ctgcgtaacc
     2941 agctagcaaa gcgttgtcgc agagattgtt gatgttgcgt ggaataccat cagaacagcg
     3001 aaagatgtaa tcgatggcgg ccggagagaa aatgccactt cgctctcctc cagcaaccaa
     3061 cagacgcgaa gtaatgtaac gatccgtttc ctcaacattt ggcaacggct ttattacaca
     3121 tctcagcgca actcgttgtt tcaactggcg caagtccgga ttgttcagca cttctctcaa
     3181 ctccggttgg ccggtcagaa cgatctgaag ttgtttcgca gtgttcgact cgtaatttga
     3241 aagaaggcga atctcctcaa gtacgtgcgg cgaaagaccc tgggcttcgt caacgatcag
     3301 aactgtccgc aagccacgag aatgccgcga ctccagtgct cgacccaact cgagcaacaa
     3361 ctcggacttt gtttcccatt tctgcagatc cagcagagtc gcaacatgtt gatagaactc
     3421 gggaacagaa agtcttggat tgaagatata ggcaaccagc accgtccgat cgaggcgctg
     3481 catcatccat cgcaggattg ttgttttgcc cgttcccacc tctcctgtca ccactaccag
     3541 gcccttgcca ctctcgattc cgtagtgcag gttcgccatc acctccgtat gcgacggcgt
     3601 gaagtagatg aatcgcggat ccggtgtaag cgcgaaaggc agttctttga gcccgtaaaa
     3661 ttcagcgtac acgaagttag acctccagtt aggaccaatt ggtccctaaa tcaatctaag
     3721 ctcgtcccga ggactggctc agcaactcaa aaacgtgagt aagcttcagg gcaagtgcaa
     3781 gcaggggaat gctcaccaga gtgacggcaa agcctgccgc cagcatcaac cgtcgtcgcc
     3841 gcggaacagc acgagcttcc tgaggcgtaa gcaactcggg caccgcaatc aacaccggca
     3901 agccagtgta gtgacgcgc
//
LOCUS       DASSFP010000253         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_964253, whole genome shotgun sequence.
ACCESSION   DASSFP010000253 DASSFP010000000
VERSION     DASSFP010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964253"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFH91_05555"
     CDS             <1..809
                     /locus_tag="VFH91_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6862488.1"
                     /translation="QRIVRIRKMTEANLIVCERDGGRASIFLNRPEKRNALSHQMIDE
                     LSDLLRQLADDTNLRVVILTGTGTAFCSGTDIAELTSATSEQAIAISKRGQKLCNQIE
                     NFPVPVVAAINGVAAGGGCELALASHIRFASSAASFSLPEVRLGIIPGYGGTQRLARE
                     VGVSRAVELMLTGRTINAGDAFELGLVNRLVAADDLFASAYELASDIEKLSPLSIRAC
                     LKAVIEGSNMALSDGLDLESALFSSLFETDDYREGTSAFLEKREPTFTGK"
     gene            1092..1574
                     /gene="ribH"
                     /locus_tag="VFH91_05560"
     CDS             1092..1574
                     /gene="ribH"
                     /locus_tag="VFH91_05560"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET6862489.1"
                     /translation="MQPIVHEGRLNAEGFRFAIVASRWNEFISGRLVEGALDALTKLG
                     ARSEQVTVFRVPGAFEIPVLAMRLAESKNFDAIICLGTIIRGQTPHFEYIANEVARGI
                     SQAGLDTGLPVIFGIVTADTVDQAIDRAGVKLGNKGFEAAMTAVELVNLYAVAFEEDR
                     "
     gene            1614..2090
                     /gene="nusB"
                     /locus_tag="VFH91_05565"
     CDS             1614..2090
                     /gene="nusB"
                     /locus_tag="VFH91_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6862490.1"
                     /translation="MGSRRKARECALQMLFAADVAEVTAPLVVQTYWAELGEPDLDET
                     ARDFATRLTTGTLANLELLDERIRSRAENWRISRMAVVDRNILRLAVYEFLFEPTPRT
                     VAINEALEIARRFSTYEATQFINGILDAIKRDLDEQQPQQNVQAEADEPDEESTAE"
     gene            2386..3567
                     /locus_tag="VFH91_05570"
     CDS             2386..3567
                     /locus_tag="VFH91_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6862491.1"
                     /translation="MSQSQTALDQAAHKPLTSLSEDEQMFRSSVREFAEGELRPRVEH
                     MDEAAKLDPAIIQQCFQLGLMGIETPEEFGGAAANFFTAILAVEELSRVDASVGVFVD
                     VQNTLVNNAFIRWGTEEQKQQYLTRLASQSVGAYALSEPGSGSDAFAMQTRAVDKGDH
                     YLLNGRKLWITNGIEADIFVLFANANPEAGYRGITAFIVEKGFPGFSIGKKENKLGIR
                     ASSTVELILEDCKVPRENLLGEAGKGYKISIETLNEGRIGIGAQMIGIATGALESALS
                     YTSERQQFGKPINQFQGVQFQLAEMATELEAARLMVYNAARMKDAGMNFVKEAAMAKL
                     YSSRVAERVSSKAIELYGGYGYVKDYPVEKYWRDSKIGAIYEGTSNMQLQTIAKLIIG
                     K"
     gene            3573..3893
                     /locus_tag="VFH91_05575"
     CDS             3573..3893
                     /locus_tag="VFH91_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862492.1"
                     /translation="MGASRIEIFRQMLANDPSNTSVLFGLAKEFEKAGQYDELVETLR
                     RYLELTDDEGNAYGMLATAYERLGREDEAKAAYERGIETAQRHGHPGMAEEYRAALAG
                     NYEH"
     gene            3883..>4645
                     /locus_tag="VFH91_05580"
     CDS             3883..>4645
                     /locus_tag="VFH91_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6862493.1"
                     /translation="MNTDTKVLKLVRKSSSSDDEDQKETTTTATAPIQLAAMPTAEAR
                     TVESSRTEFTVAPKTLRSERDALIGMYRTMYMSRRLDDKEIQLKGQNRIYFQISGAGH
                     EAILIAAGVALKPGYDWFYPYYRDRALCLQLGMTPLEQLLSAVGAEADPNSHGRQMPS
                     HWGHKKLNIVSQSSPTGTQLLQAVGCAEASYRIELIPELKRVVKDFHDDEVAYVSLGD
                     GTSSEGEFWEALNTASNLKLPVVFLLEDNGYAISVP"
BASE COUNT         1203 a         1128 c         1194 g         1120 t
ORIGIN      
        1 ttcagcgaat cgttcggatc agaaaaatga cagaagcgaa tttgatcgtt tgcgaacgag
       61 acggtggacg agcctcgatc ttcctcaatc gacccgaaaa acggaacgca ctatctcatc
      121 aaatgattga cgaactgtct gatctgctta gacagctcgc cgatgatacc aacctgagag
      181 ttgttattct cactggaacg ggaacggctt tttgttccgg cacggacatc gcagagctaa
      241 cttcagcaac ctcggaacaa gctatcgcaa tatcaaagcg cggacagaag ctatgcaatc
      301 aaattgagaa ctttccggtg ccggtcgtag cggcgattaa tggcgttgca gccggtggcg
      361 gttgtgagtt ggctttggct tctcacattc gtttcgcctc gagtgcagct tcgtttagtt
      421 tgccggaggt tcgactgggc atcattcctg gctacggagg cactcaacga ttagcccggg
      481 aagtcggggt gagtcgcgcg gtcgaattga tgttgactgg caggacaata aatgcgggcg
      541 atgcgtttga gcttggctta gtcaatcgat tagtcgctgc ggacgatctg tttgcatctg
      601 cgtatgaatt ggcaagcgat attgagaagc tttctcctct ctcgattcga gcttgtctga
      661 aggcagtaat tgaaggctcc aacatggcac tctccgacgg cctcgatctt gagagcgctc
      721 tattttcctc gctgtttgag actgacgact atcgtgaagg cacatccgcg ttcctggaga
      781 agcgggagcc aacctttacg ggcaagtaaa tgaaacaaca acacagtaga agaaatcact
      841 gatcaattat gatttgataa ttatgaatta ttatttttcc cgattcccgg gtgctcgcgc
      901 aaaaggccca gagtgaacgt gcgaattagt aattgacaaa tatcaaatga taattcacca
      961 atgatttcat cgtatcctcc aatgcctaaa cctttggcga acagcgcaat cctcaacggc
     1021 aaacttgatg cacctcaaag cattccctat actgaaaaag tattttgatc ctgatcgcgg
     1081 agtcgagttg tttgcagcca atagttcatg aaggtcgttt gaatgcagaa ggctttcgtt
     1141 ttgcgattgt cgccagtcgt tggaatgaat tcatttctgg ccggctggtg gaaggcgcgc
     1201 tggatgcatt aacaaaactt ggcgcgcgat cagagcaggt gaccgtgttt cgtgttcccg
     1261 gcgcatttga gattccagta cttgcgatgc gtctcgcaga gtcaaagaac tttgacgcaa
     1321 tcatttgttt aggtaccatc atccgcggtc agacgcccca cttcgaatac attgcaaatg
     1381 aagtggcgcg tggaatctct caagccggtc tcgataccgg gttgccagta atctttggga
     1441 tagtaactgc tgatactgtc gatcaagcga tcgatcgagc cggcgttaag ctcggtaaca
     1501 aggggtttga agccgcgatg actgcggtgg aactagtaaa tctttacgcg gtagcttttg
     1561 aggaagatcg atagagatcc gcatctctaa attcgatctc tattctgagt attatgggtt
     1621 cgcgacgtaa ggcgcgtgaa tgcgccttgc aaatgctctt tgcggctgat gttgccgaag
     1681 tgactgcgcc tctcgtggtc caaacctatt gggctgaact gggggagccg gatctcgatg
     1741 agaccgcgcg cgatttcgca actcgcctca ctactggcac gttggcaaat ctggagttgc
     1801 tcgacgagcg tatccgttcg cgtgctgaaa attggcgtat ctcgcgcatg gcggtggttg
     1861 accgcaacat cctccgctta gctgtttacg aattcctctt tgaaccgacc ccgcgtacag
     1921 tcgctattaa cgaagctctc gaaatagctc gccgcttctc aacgtacgaa gcgacccaat
     1981 ttatcaacgg tatactcgac gctatcaaac gagacctcga cgaacaacag cctcaacaaa
     2041 acgtccaagc tgaagccgac gaacccgacg aagagtcaac ggctgagtag cggcgcgagt
     2101 ccggttattt tccatttatc atttttcatt ttccattttt catttccgaa cgtagcgtgt
     2161 tcaggcaaag atagtggccg gcggaatctg acggtgggtg gcggaacggc cgaaaaagtg
     2221 ttgtcccatc tcccacaatg tctcttaagg ccaacatcca cccgaaaaat gaaaaatgaa
     2281 aaatgaccaa tgataaatgg aaaatggttg gggcttttgc ctttttactt ttggcttgat
     2341 tatcatgctc tgtccaacta gaacgccaac cccaaacagg tcattatgag tcaatcccaa
     2401 acagctttag atcaagcggc ccacaagcca cttacgtccc tctctgaaga cgaacagatg
     2461 ttccgatcaa gtgttcgcga gtttgccgaa ggagaacttc ggccgcgcgt ggaacacatg
     2521 gacgaagctg caaaacttga cccggccata attcagcagt gcttccaact gggattgatg
     2581 ggaatcgaaa cgcccgaaga gttcggaggg gctgctgcca atttctttac tgccattctc
     2641 gctgttgaag aactctccag agttgacgcc tcagtcggcg ttttcgtcga tgtgcaaaat
     2701 actctggtta acaacgcgtt tattcgctgg ggaaccgaag agcagaaaca gcagtacctg
     2761 accaggttgg cgtctcaaag tgtcggtgct tacgctctga gcgagccagg ttcaggttct
     2821 gacgcgttcg ccatgcaaac gcgtgccgtc gacaagggcg atcactattt gctgaatgga
     2881 cgcaagcttt ggatcacgaa cggaattgaa gccgatatct ttgttttgtt tgctaacgca
     2941 aatccagaag ctgggtatcg aggtatcacg gctttcatcg ttgaaaaagg ctttcccggt
     3001 ttctcaatcg gaaaaaagga gaacaagctc ggcatccgcg cttcatcgac agttgaactg
     3061 attctcgagg actgcaaagt gcccagggaa aacctactcg gcgaagctgg gaaaggctac
     3121 aagatctcca tcgagacttt gaacgaaggt cgcatcggta tcggagcgca aatgattggc
     3181 atcgccaccg gagctctcga aagcgctctc tcgtacacct ctgaacggca acagtttggc
     3241 aaacccatta atcaatttca gggtgttcag tttcaacttg ctgagatggc caccgaactt
     3301 gaagccgcca gactgatggt ctataacgcc gctcgaatga aggacgcggg catgaatttc
     3361 gttaaggaag cggcaatggc taaactttac tcgtctcgcg tcgccgaacg cgtaagttca
     3421 aaagcgatcg agctctatgg cggttatggt tacgtaaaag actatccagt tgagaagtac
     3481 tggcgcgact cgaagattgg tgcaatctat gaagggactt cgaatatgca gcttcagact
     3541 attgccaaac tcataatcgg aaagtgaact aaatgggtgc ctcaagaata gaaatctttc
     3601 gacaaatgct ggctaacgac ccgtcgaaca ccagcgtgct tttcggactc gccaaagaat
     3661 ttgaaaaggc cggacagtat gacgaactcg tagagacatt acgccgctat cttgaactta
     3721 ccgacgacga gggcaacgcc tacggtatgt tggcaactgc ctatgaacgc ctgggtagag
     3781 aggacgaggc aaaagctgcg tacgaacgcg gtattgagac tgcccaacgt catggacacc
     3841 cgggaatggc tgaagagtac cgcgctgcat tggctggcaa ctatgaacac tgacacaaaa
     3901 gtattgaagc tggtacgtaa gtcttcatca tcagatgatg aagatcagaa agagacaact
     3961 acgacagcga ccgctcctat tcaactcgct gcaatgccga ctgctgaggc cagaactgtc
     4021 gaaagctctc gtaccgagtt taccgtcgcc ccaaaaactt tgcggtccga acgcgatgct
     4081 ctcatcggca tgtatcggac gatgtatatg tcgcgccggt tggacgataa agagatacag
     4141 ctgaaggggc agaaccggat ctactttcag atcagcggtg cgggtcatga agcaatactt
     4201 atagcggcag gagttgccct taagcccggt tacgactggt tctaccctta ctatcgtgac
     4261 cgcgctctct gtctgcaact tggaatgacg ccactcgaac aacttctttc tgcagtaggg
     4321 gcggaagcag atccaaactc tcatggacgc cagatgcctt cgcactgggg ccacaagaaa
     4381 ctcaacatag tctcacaatc atcaccaaca ggaactcagt tgctgcaggc tgtaggctgc
     4441 gccgaagcaa gttatcgaat tgaactcatt ccagaattga aacgggtcgt taaggatttt
     4501 catgacgacg aagttgctta tgtatctctc ggagatggaa cgagtagcga aggtgagttt
     4561 tgggaggcgc tgaacaccgc cagcaatttg aaactacccg tcgttttcct tttggaagat
     4621 aacggttacg cgatcagtgt tccgg
//
LOCUS       DASSFP010000254         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1401732, whole genome shotgun sequence.
ACCESSION   DASSFP010000254 DASSFP010000000
VERSION     DASSFP010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401732"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFH91_05585"
     CDS             complement(<1..666)
                     /locus_tag="VFH91_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6862494.1"
                     /translation="MRKTTYLCLFLFLLSASSSVLAQSNYASLSGTVFDPQKRVIPGA
                     SLQLTSESTHASRLITSNEQGVFQFTALLPAEYKLTVQAPGLASLNQSVTLEVGQQMT
                     LDVSLNLSSLNTTVDVNTDIANVLRTTDASVGEVVEPTSVRNLPLNGRMLIDLVLTVP
                     GAHESHGAQAGDMSALYWRPGQRSAVSISGNRPNANYFLLDGATNTDPTFNTLNFSPS
                     PDSV"
     gene            995..>3541
                     /locus_tag="VFH91_05590"
     CDS             995..>3541
                     /locus_tag="VFH91_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET6862495.1"
                     /translation="MTIKPQTRQEDSAKVATAVDPVCGMTVNSTTAAGSYEYKGQTYY
                     FCATNCLEKFRLDPERFLNKPHESMMPQLIGIGPKASGPELTGATYTCPMHPEVRQEK
                     LGSCPKCGMALEPLTVSPPKQKTEYTCPMHPQVVRDAPGSCPICGMALEPRTISLTEE
                     ENPELVDMRRRFWVSAVLTLPLFLIGMSELIPGAHIARLIPMRGLNWIELALATPVVL
                     WGGWPFFERAWQSVVNRSPNMFTLIGLGVSVAYVFSVMATIWPQIFPGTFRDPMGNVP
                     VYFEAAAVITTLVLLGQVLELKARSQTGAAIKALLGLAPKTARRVTADGSERDTPLEQ
                     VEVGHLLRVRPGEKIPVDGVVIEGSSTIDESMVTGEPIPVEKLSGDRVIGATVNGTGS
                     FVMRAERVGAETLLSQIVQMVAEAQRSRAPIQKLADVVAAYFVPTVIAIAVVTFIVWS
                     IWGPEPQLAHGLVNAVAVLIIACPCALGLATPMSIMVATGKAAQFGVLFKNAEAIEVM
                     RKVNTLIVDKTGTLTEGKPKLTSVVTASGIDEKMLLRLAATLERGSEHPLAAAIVAGA
                     EEREIELAKTNLFESITGKGVKGEVDGRQVALGNRAIVDDLGLDPGTLASNAEELRSD
                     GQTVVFVIIDDQVAGLIGVADPIKETTPEAIKRLHEEGIRIVMVTGDSATTANAVAKT
                     LGLDEVVAEVLPNQKVDAVKRFQSEGRIVAMAGDGINDAPALAQADVGVAMGTGTDVA
                     MKSADITLVRGDLRGIVRARVLSRLTMSNIKQNLFFAFVYNSIGVPIAAGVLYPFFGI
                     LLSPMIAAAAMSFSSVSVIGNALRLRRANLSFSRQSSPELLVRSVPKRGSV"
BASE COUNT          849 a          856 c          986 g          850 t
ORIGIN      
        1 cacactatcg ggtgacggac taaaatttag agtattgaac gtcggatctg tgttagttgc
       61 gccgtcgagc agaaaatagt ttgcgttcgg ccgattaccg ctaatgctta ctgccgagcg
      121 ttgaccggga cgccagtaga gcgcactcat atcacctgct tgtgcgccat gactttcatg
      181 cgcaccgggc accgttaaca ccagatcgat cagcatccga ccgttgagtg gcaggttgcg
      241 aacagaggta ggctcaacga cttctcccac actcgcatca gtagttcgca gcacgttagc
      301 tatatccgtg ttgacatcca ccgtagtatt gagagaagag aggttgaggc tcacatcaag
      361 agtcatttgc tggcccactt cgagcgtgac gctctggttc agcgacgcga gtcctggcgc
      421 ttgcacggtc agtttgtatt ctgccggcag cagagctgtg aactgaaaaa ccccctgctc
      481 gttgctggtg atgaggcgtg atgcgtgtgt actttctgag gttagttgca atgaagcgcc
      541 gggaatcact ctcttttgcg gatcgaaaac ggttccgctc aaacttgcgt aattactctg
      601 tgccagaacg ctggaagaag ctgacaggag aaagagaaat aaacacaagt aagtcgtttt
      661 tcgcattaga cctccgcctg cagtcgcgat cgttgcttac tattgaacgg tctcggattt
      721 gatgaacggg accaaattat gctgaatcaa gatcgtggat tcagttctgc aatcagttag
      781 ggatcccagc ccctgaacac caaatcgaga gttgggaaat tgtagagggc caaaactcta
      841 acaggcggca gtgctctggt catgaaccaa aaggttgggc ttgttatcaa gctttagttg
      901 tagagcgatc gcaagtcaag ttaggactct accaacagat cgcgtagcct tgactctgta
      961 gccaagtaca gagtttataa tctccccatc tttgatgacg ataaaaccac agaccaggca
     1021 ggaagatagt gcaaaggtcg ccacagctgt agatcctgtt tgtggcatga ccgtgaattc
     1081 caccacagct gccggctctt atgagtacaa agggcagacc tactattttt gcgctacaaa
     1141 ttgtctggaa aaatttcgac ttgaccccga acggtttctc aataaaccgc acgagtcaat
     1201 gatgccgcag ctaatcggca ttggtccaaa ggcgagtggt ccagaactca ccggcgcgac
     1261 ctacacctgt ccaatgcacc cggaggtccg tcaggagaag ctgggttcgt gccccaagtg
     1321 cggcatggcc ctcgaaccgc ttaccgtcag cccaccaaaa cagaagactg aatacacctg
     1381 tccaatgcat ccgcaagtcg tgcgggatgc gcccggcagt tgtccgatct gcgggatggc
     1441 tttggaaccg cgaacgattt ctttaacgga agaagaaaat cccgaactcg tcgacatgcg
     1501 ccgcagattt tgggtgagcg cggtcttaac ccttccattg tttctaatcg gcatgagtga
     1561 gcttattccg ggcgcgcata tagctcgctt gattcccatg cgcggtctga actggatcga
     1621 actcgcgcta gctacgcctg ttgtgctttg gggtggctgg ccattttttg aacgtgcgtg
     1681 gcagtcagta gtcaatcgca gtcccaacat gtttacgttg attggacttg gtgtgtcagt
     1741 ggcatatgtt ttcagtgtca tggcgacaat ctggccacag atttttccag gtacttttcg
     1801 tgacccaatg ggaaatgttc ccgtctactt tgaagcagcg gcggtaatca caacgctggt
     1861 tctactgggc caggtcttgg agttaaaagc tcggagccag accggtgccg cgatcaaagc
     1921 tttactcggt ttggcgccaa agaccgctcg tcgcgttaca gctgacggca gtgagcggga
     1981 tacacctctt gagcaagttg aggtgggcca tctcttgcgt gttcgacctg gggagaagat
     2041 tccggtcgac ggagtagtta ttgaaggctc aagtacgatc gacgaatcaa tggtaaccgg
     2101 cgaaccgatt ccggttgaga aactatcagg cgatcgtgtc atcggagcga cagttaacgg
     2161 caccggtagt ttcgtgatgc gcgctgaacg ggtcggcgct gaaactctac tttctcaaat
     2221 tgtgcagatg gttgcggaag cacaacgcag ccgtgcgcca attcaaaaac tggcggatgt
     2281 tgttgccgcg tacttcgtac cgacagtgat agcgattgcg gtagttactt ttatagtttg
     2341 gagtatctgg ggtcccgaac cacaactagc gcacgggctg gttaacgctg tcgcagtact
     2401 gatcatcgct tgtccgtgtg cactcggact ggcgacgccg atgtcgatta tggtggcgac
     2461 cggtaaggcg gcccagtttg gagtattgtt caaaaacgct gaagccattg aggtcatgcg
     2521 taaagtgaac accctcatcg tcgataaaac cggcacgcta actgaaggca aaccaaaact
     2581 gaccagtgtc gtcactgctt cgggaattga tgagaagatg cttttgagac tggcagcaac
     2641 actggaacga ggaagcgaac acccgttggc ggcagctatc gtcgcgggtg ctgaggaacg
     2701 cgagatcgaa ctggcgaaga caaacctctt cgagtccatc acgggtaaag gtgtgaaagg
     2761 tgaagttgac ggccgtcaag tggcccttgg taaccgcgca attgttgatg atctcggact
     2821 cgatcccggc actcttgcgt caaacgcaga ggagcttcgt tcggatggac agactgttgt
     2881 gttcgtcatc atcgatgacc aggttgcagg attaattggc gtagctgatc cgattaaaga
     2941 gaccactccg gaagcgataa agcgacttca tgaagaaggt attcgcattg ttatggtgac
     3001 tggtgacagc gctacgactg cgaatgctgt cgcgaaaact ttaggcctcg atgaagtagt
     3061 agcggaggtt ctgccgaatc agaaagttga tgcggtgaaa cgatttcaaa gcgagggccg
     3121 aatcgtcgcc atggccggtg acggaataaa cgatgctccg gcattagctc aggctgatgt
     3181 cggcgttgcc atgggaacgg gaacggatgt ggcaatgaaa agtgccgaca ttacgttggt
     3241 aagaggagac ttgcgcggaa ttgtacgcgc gcgagttctg agccgtttga cgatgagcaa
     3301 tatcaaacaa aatcttttct ttgcgttcgt ttataactct ataggagttc caattgctgc
     3361 gggagttctc tatccgttct tcggaatcct gttaagtcca atgatcgctg cagcggcgat
     3421 gagcttcagt tcggtatcgg tcattgggaa tgcgctgcgt ctccgtcgcg ctaatctatc
     3481 cttctccaga caatcgtcac ctgagttact ggtcaggtca gtaccgaagc gcggtagcgt
     3541 c
//
LOCUS       DASSFP010000255         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_217750, whole genome shotgun sequence.
ACCESSION   DASSFP010000255 DASSFP010000000
VERSION     DASSFP010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217750"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFH91_05595"
     CDS             <1..223
                     /locus_tag="VFH91_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6862496.1"
                     /translation="DKLKKYASEVGLDRALFDEALDEGTFHDQVQMDIIEAGKIGVID
                     TPTFFVNGRRTTGISYAELKLDIEKALQK"
     gene            319..990
                     /locus_tag="VFH91_05600"
     CDS             319..990
                     /locus_tag="VFH91_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862497.1"
                     /translation="MRRLPDIQRKGQRKALFAASSLVLLLATQSLAQSGRRTSKPSQT
                     PTPTPELQQDPAALPDKESGRGLTQKVSLLVAGQFSSKHLLSEDAILSNMISHLSEWK
                     NIKATSLGTLKHSEATKRAKLESEQFVVLVQFDVDEFQSGTIILNSPDLDVRILVFAP
                     QTGQKKFEGKVYYKAVGGPMLKKDNWPNGTPIRITTEAVGIEAAEQIRDWLIVAESRR
                     AAGKN"
     gene            complement(994..2079)
                     /locus_tag="VFH91_05605"
     CDS             complement(994..2079)
                     /locus_tag="VFH91_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6862498.1"
                     /translation="MTAARQITESTYMHWAKTSSQAQFNLATSGVGNLSIHDLDVSLD
                     DLELTGGGYGYGPLMELIGKRYDVATESIVTAAGTSFANHLAMAALIKPGDQILFEHP
                     AYEPMLATARYFGADVKRFSRRFDNNFDVDLDDLRSNLTRDTRLIVLTNLHNPTSVLI
                     DQATLLQIGKMASEIKARVLVDEVYLETLFESQPRTVFHLGKEFVVTSSLTKAFGLSG
                     LRCGWILAEPELATRMWLLNDLFASTPVHVGERLSVLAFQQLERIAAQAKSLLNRNRQ
                     LVFEFLDSSPQLEVVRPDFGTTVFPRLAGRDVEAFCELLREKFQTSVVPGSFFEMPDH
                     FRLGFAGDTESLKAGLERLQSALAEFA"
     gene            complement(2076..>2945)
                     /locus_tag="VFH91_05610"
     CDS             complement(2076..>2945)
                     /locus_tag="VFH91_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6862499.1"
                     /translation="STVQSILMVLKILAIIMLVGCGLLLARSVPGSAPLLDQPPSLGL
                     LTAFGAAMVPVLFSYGGWQTATFVAGEIKEPSKNLPRGLILGVGGVVVLYLAANVVYL
                     RVLGVSGLAASKAPASEVMRLAMGDVGAVAIAAGIAISTLGFLSQSMLTAPRVYFAMA
                     QDGIFFKSVGAINARTKAPVVAIALQGALAIVIALVGKYEQILDYVVSMDFIFFGLTA
                     CCIFIFRSRGTADQRFTRIPAHPVSTIVFILVCWLVVINTIYHNPRNTLIGVAILLAG
                     VPAYMFWARRSKL"
BASE COUNT          836 a          757 c          722 g          630 t
ORIGIN      
        1 tgataaactt aagaagtatg cgtcggaagt aggactcgat agagcattgt tcgacgaggc
       61 ccttgatgag ggaaccttcc acgaccaggt ccagatggat attattgagg caggtaaaat
      121 tggtgttatc gatacgccaa ctttctttgt aaacggaagg cgaacaaccg gcatcagcta
      181 cgctgaactt aagctagaca ttgaaaaggc cctgcaaaaa tagtcgcggc attccgccga
      241 tattgcagtc gcacggcttg cacggtaaac ctatttatcg ctcactgctt ctaaagccca
      301 aaccgctgga gagattctat gagaagactc cccgatattc agcgaaaagg ccaacgaaaa
      361 gcgctctttg ctgcttccag cctcgtcttg ctgctggcaa cgcaatccct ggctcagtca
      421 ggcaggcgca catcaaagcc ctctcaaaca ccaactccga cacccgaact acaacaagat
      481 ccagccgcgt tgcctgacaa ggaaagcgga cgaggcttaa cacaaaaggt ctcgttacta
      541 gttgccggcc aattcagttc gaaacatctg ttaagcgaag acgccattct ctcaaacatg
      601 atcagccatc ttagcgagtg gaaaaacatc aaggcgactt ctctcggtac gctgaagcat
      661 agcgaagcaa ccaagcgtgc aaagttggaa agtgagcagt ttgtagtgct ggtccagttc
      721 gacgttgatg agtttcaaag cggtaccata attctgaact caccagacct cgacgtgcga
      781 attttggtgt ttgcgccaca aaccggtcag aagaaatttg aggggaaggt ttattacaag
      841 gctgtcggtg gtccaatgct aaagaaagac aattggccca atggaacacc gattagaatc
      901 acgaccgagg ctgtgggaat tgaggcagcc gaacagattc gggattggct gatagtcgca
      961 gagtcaagac gagctgcggg taaaaattaa tcctcaggca aactcagcta gcgcggattg
     1021 aagacgctcc aaaccggcct tgagcgactc tgtgtctcct gcaaatccaa gtcggaaatg
     1081 atctggcatt tcaaagaaag atccagggac aactgaagtc tgaaactttt ctcgaagtaa
     1141 ttcacaaaaa gcctcaacat cgcgcccggc aagacgtgga aacacagtcg ttccgaaatc
     1201 agggcgaact acttcaagtt gcggcgagga gtcgagaaac tcaaaaacaa gttgtcggtt
     1261 gcgatttaag agagactttg cctgagcggc aatcctttcg agttgctgaa acgccaggac
     1321 actcagccgc tcacccacat gcactggggt ggaagcaaat aagtcgttaa gcaaccacat
     1381 gcgagtagcc agttcaggct cagccaatat ccaaccgcat ctcaagccac ttagcccaaa
     1441 ggctttggtc aaactgctgg taaccacaaa ttccttgcca agatgaaaca ccgttcgcgg
     1501 ctgagattcg aagagggttt ccaaatagac ctcgtcgacg agtacacgtg ccttgatctc
     1561 acttgccatt ttcccgatct gcaagagtgt cgcttgatcg atcagcacac tggttgggtt
     1621 gtgcaaattc gtgaggacga taagacgagt atcccgtgtc aggttcgatc ggaggtcatc
     1681 cagatcaaca tcaaagttgt tatcaaatcg tctggaaaag cgttttacat ccgctccaaa
     1741 gtaacgcgcc gtggcaagca tcggttcata tgcaggatgt tcaaacagga tttggtcgcc
     1801 tggcttaatc aacgctgcca tggccagatg gttggcgaaa ctggtaccag cggcagtcac
     1861 aatcgactcg gtggctacgt cgtaccgctt gccgatcaat tccatcagtg ggccataacc
     1921 atatccgccg ccggttagtt caagatcatc caacgacacg tcgagatcat gaatacttaa
     1981 attgcctaca ccgctggtgg caagattgaa ttgagcttga gaactggtct tcgcccaatg
     2041 catataagtt gattcagtta tttgacgagc agccgtcaca gcttcgacct cctggcccaa
     2101 aacatgtaag cagggacacc tgccagcaga atggcaacac caatcaaggt attcctggga
     2161 ttatggtaga tggtgttgat aacaaccagc caacacacga gtatgaaaac tatggttgat
     2221 actggatgag ctggaatgcg cgtaaagcgc tgatcagcgg taccgcggct gcgaaagatg
     2281 aaaatgcagc aagcagtaag tccaaagaaa atgaagtcca tcgaaactac gtaatccagg
     2341 atctgctcgt attttccgac cagcgcgatg acaattgcga gtgctccctg taacgcgatc
     2401 gcaaccaccg gagcttttgt acgagcattg attgcgccta cgctcttgaa aaatattcca
     2461 tcctgggcca tcgcaaaata gacccgcggc gcggttagca tgctctggct aaggaatcca
     2521 agggtcgaaa tcgcaatgcc tgcagctatc gcgacggctc caacatctcc catggcgagt
     2581 cgcatgacct ctgacgctgg cgccttcgat gccgccagtc ccgacacacc taagacccgc
     2641 agataaacta cattagcagc cagatataga accacaacac cacccacacc gagaattaag
     2701 ccacgcggta aattcttcga tggctctttg atctcgcctg caacaaaagt cgctgtttgc
     2761 cagccgccgt aagagaaaag cacggggacc attgctgctc caaaagcagt cagcagtcca
     2821 agggaaggtg gttggtccaa tagaggagct gagcctggaa cgcttctagc caacaggagg
     2881 ccgcatccta ccagcattat gatcgcgagg atcttcagca ccatcaaaat actctggacc
     2941 gtgct
//
LOCUS       DASSFP010000256         4621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1259967, whole genome shotgun sequence.
ACCESSION   DASSFP010000256 DASSFP010000000
VERSION     DASSFP010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259967"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1263
                     /locus_tag="VFH91_05615"
     CDS             382..1263
                     /locus_tag="VFH91_05615"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET6862500.1"
                     /translation="MPNPATQMTLESLRSVPLFASLDDSAAIHLRDLLSEQEVPSNTR
                     LFNQGDQGNAMYLIESGRVRISIRDEEKQELTLAELAQGDFFGEMSIIDGRQRSADAN
                     VIEDSRLAILSRDAFLTFVRSNPDVALEMLSALSDRLRRTDQLLRSRVSKNANEEEKA
                     RLTIADRAADLIAEFGGSWKFIGVSIALIIFWIIFNSYILVKGFDPVPYQMLNLVLAV
                     IAGMQAPIIMMSQNRQGEKDRLRADLDYQVNLKNELSLTEVLRRLDVIESERLPRLFE
                     EQNLKLSNLKVNIDVED"
     gene            1266..1964
                     /locus_tag="VFH91_05620"
     CDS             1266..1964
                     /locus_tag="VFH91_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET6862501.1"
                     /translation="MLQAIFFDFNGVIIDDESIQLKAYQQVLREHQVDLTEEWYFDAL
                     GMDDKTFVRAMFERAKQPLSEENFNKVLSEKTELHRKMIEDELPLFPGILNFLKSTAR
                     HFSLGLVSMANPVEIGYVFERAHLKSLFSVVITSEDVTRCKPAPDCYAEGLAKMNTAR
                     QHKRLLPLLAEECLAIEDSPPGIRAAAEVGMKTLGITNTVKESELRDAGADVVTHSLA
                     DWTVDSVKLVFDRH"
     gene            1968..3431
                     /locus_tag="VFH91_05625"
     CDS             1968..3431
                     /locus_tag="VFH91_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6862502.1"
                     /translation="MSFIDILFLAFRNLRQAKLRASLTTMGVIVGVAVIVTMVSFGLG
                     LQRNMLARFKALDLFNEIQVFGQGLSNLAGLDRPTRRDEGERRNARQRADKNPTRILD
                     DAGVKEISAIPGVSYVEPSVNFGVYVRSNGKLLTQSVGGANIPNASTRFQQFAAGHMI
                     SAPSADEAVVTERFIRDYGYATPADAVGKTLELLAPPDEPRDQSKKEEEESTPNFFGI
                     PLDDPGLDESDSSLETKTFRISGVLNTEIKEGAGQGGLRGLMPGAGIYIPLNVAHQWS
                     TQHRGPLSRVAFALARQGGNLGEGQTEGYESAVVRVKDPVQLTEVRKRITELGFGSFS
                     IVDELDQIRTVFLIIDSVLGLLGGISLLVASFGIANTMIMSILERTREIGIMKAIGAE
                     DREIKLIFFFEAAVIGIFGGVLGTLLAWGIDGIANKLAYRFILKPQGASAIDFFSLPP
                     YLTLGAILFALIVSILAALYPAARAARIDPVRALRHD"
     gene            complement(3583..4152)
                     /locus_tag="VFH91_05630"
     CDS             complement(3583..4152)
                     /locus_tag="VFH91_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6862503.1"
                     /translation="MDSRYYPSVEVDIPNLSAELRNLFDSDFEVQAFQVSSTNIFQAR
                     KSNTLRDLTGLSSALTIKITPEAGGTRVEMGMQKWFDKAAVAAVGAIVFAPLLALAAL
                     GAYWQYRLTEDAWKIIEDHIARKAGGYVPPLPGRCGTCGAANVAGSDFCSACGANLKI
                     GRSCQSCGAIQKESTARFCNKCGKPLGVS"
     gene            4263..>4621
                     /locus_tag="VFH91_05635"
     CDS             4263..>4621
                     /locus_tag="VFH91_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862504.1"
                     /translation="MMRSLITCLLLLSFASSLVYSQSAPTSRSIDGFSTQTTIDERQL
                     EEKFRAIPNPASAREHLRQLTMEPHIAGSKEDYATAIYVRDQLKSYGLSAELKEYEVW
                     LNYPKSPSVLELITNRR"
BASE COUNT         1128 a         1104 c         1193 g         1196 t
ORIGIN      
        1 acgatggtct aactatcgtg atggttaccc acgatcagga gttggcaagc agctttgcgc
       61 accggacggt cttcatgaaa gatggaagag tggtctaatt tcgacggacc atgagtcagg
      121 tggtaacacg gtcagtatcg attctcagta cgaacaaagc cttggatgat tatcatttga
      181 taattgatga ttgatatttg agaattttca attttagcgc gctctcggca aaacgaagta
      241 gcaattctca aatgtcaaat atcaattatc aaatgtaatc attctggatt gttcgtaccg
      301 cgaatcggca ctgaccaccg tgtcaaccaa gcagctgaac cttcgacttg ttgatcgctg
      361 tgtacttttt tgggaggaaa tatgcctaac cccgccactc aaatgacgct cgaatctttg
      421 cgctcagtac cgctctttgc ttctctcgat gactctgcgg caatacattt gagagatctt
      481 ctgtccgagc aggaagtgcc gagcaataca cgcctcttta atcagggcga tcagggcaat
      541 gctatgtatc tgatcgaaag tggtcgtgtg cggattagca ttcgagatga agagaagcag
      601 gaattgacgc tggctgagct cgcacaggga gattttttcg gcgaaatgtc gattatcgat
      661 ggccggcaac gttcagctga cgcgaatgtg attgaggatt cccggctcgc tattttgtcg
      721 cgagacgcat tcttaacctt tgtgcgaagt aatccggatg tcgctttgga aatgttgagt
      781 gcgttgtctg accgtctgcg ccggactgat caacttcttc ggtctcgtgt ttcgaagaat
      841 gccaacgaag aagagaaggc tcgcttgacg atcgccgatc gagctgccga tttgatcgct
      901 gaatttggcg gcagctggaa atttatcgga gtctcaatcg cactgattat tttttggatc
      961 attttcaact catacattct ggtgaaaggc ttcgatcccg ttccttatca gatgttgaat
     1021 ctggtcttag cagtaattgc cggcatgcag gccccgatca ttatgatgtc gcaaaaccgg
     1081 cagggagaaa aggatcgact ccgtgcagat cttgattatc aggttaactt gaaaaacgaa
     1141 ttgtcactca cggaagtctt gcgccggttg gatgttattg agagtgaacg cttgccgagg
     1201 ttattcgaag agcaaaacct aaagttgtcc aatctaaaag tgaatataga cgttgaagat
     1261 tgattatgtt gcaggccatc tttttcgact ttaatggagt gatcatcgac gacgagtcga
     1321 ttcaattgaa agcgtatcag caggttttgc gcgagcacca ggtcgacctc accgaggagt
     1381 ggtatttcga tgcgctcggc atggacgaca agaccttcgt gcgtgcgatg ttcgagcggg
     1441 caaagcaacc gctgtcagaa gaaaacttta ataaggtttt gagtgagaaa acagaactgc
     1501 atcgaaaaat gatcgaagat gagcttccgc tctttcccgg aatcctcaac ttcttaaaga
     1561 gcacagcacg ccatttttct ctgggtcttg taagcatggc gaatccagtt gaaattggat
     1621 acgtcttcga acgagcccat ctcaaatcgc tcttctcagt cgtaataaca tcagaggacg
     1681 tcactcgatg taagccagcg cctgactgtt atgcagaagg gctcgcaaag atgaacacgg
     1741 ctcggcagca caaacgactg ttaccactgc tggctgaaga gtgtttagcg atagaagatt
     1801 cgccgccggg gattagagct gctgcagagg ttgggatgaa aacgcttggc attacgaata
     1861 ctgtcaagga atcagaactg cgtgacgccg gcgcagatgt cgtaacacac agcctggctg
     1921 attggaccgt tgattcggtc aagctcgttt ttgataggca ctgacgcatg agttttatcg
     1981 acatcctctt tctcgcgttc cgaaatttgc gacaggccaa actgcgcgca tcgttgacca
     2041 ccatgggggt cattgtgggc gttgctgtca tcgtaaccat ggtctcgttt ggtctgggtt
     2101 tgcagcgcaa tatgctggct cgttttaaag cgctcgacct gttcaacgaa atccaggtct
     2161 ttggccaggg cttgagcaat ctcgctggac tcgatcgacc tacgcgtcgt gatgagggag
     2221 aacgacgcaa tgcacgacaa cgtgccgaca aaaatccgac gcgaattctg gacgacgcgg
     2281 gggtcaaaga aatttctgcg atccccggag tctcttacgt tgaaccaagc gtgaatttcg
     2341 gtgtttacgt ccgatcaaac ggaaaactat taactcaatc tgttggtggc gccaatatcc
     2401 cgaatgcatc gacaagattt caacaattcg cggccggaca tatgatttca gctccgtctg
     2461 ctgatgaagc agtggtgaca gagcgattca tacgcgacta tggttacgca acgccagctg
     2521 acgccgttgg caaaactctt gaactgttgg cgccgcccga tgaaccacgc gatcaaagta
     2581 agaaagaaga agaggaatcg actccgaact tttttggaat ccctttggat gaccccggcc
     2641 tggacgaatc ggattcaagt ctggagacaa agacatttcg gatctcaggt gtcttgaaca
     2701 ctgagatcaa agaaggcgca gggcagggag gcttgcgtgg attgatgcct ggcgccggca
     2761 tttacattcc attgaatgtg gctcaccagt ggtctactca acatcgcgga cctttgagtc
     2821 gcgtcgcttt tgcactggcg cgtcaaggcg gcaatctggg tgaaggacag actgagggtt
     2881 acgagtcggc tgtcgtgcgc gttaaggatc cggtccagct cacagaagtt agaaaacgaa
     2941 ttaccgaact tgggtttggg agtttctcaa tcgtagatga acttgatcag attcgcactg
     3001 tgttcctgat catcgattca gtactcggtc tgcttggagg catctcattg ttggttgcgt
     3061 cctttggaat tgccaatacg atgatcatgt cgattctcga gcgcactcga gagattggca
     3121 tcatgaaagc gatcggtgct gaagatcgag aaataaagct gatcttcttc tttgaagcgg
     3181 ccgtgatcgg aatctttggt ggtgttttgg ggacgcttct ggcttggggt attgatggta
     3241 tcgcgaacaa gctggcttac aggttcatcc tcaaaccgca gggtgcctcg gccatcgatt
     3301 tcttttcatt gccaccatat ttgaccctgg gagcgattct gtttgctcta atagtttcga
     3361 ttctcgcggc gttgtatccg gcagcacgcg ccgctcgcat cgatccggtc cgcgcgttac
     3421 gacatgattg agattggatc cgcggtcagt accgattgtc cgatatgctt tattagcttg
     3481 tcgtgttcgc gtttggggct attcgaaatg aaccttaatg cactaagagc cgacaagcta
     3541 aagcatatcg gacaatcggt accgaccgcg cctccgcccc gattacgaga cgccgagggg
     3601 tttgccgcac ttgttgcaga atcgggcagt tgattccttt tgaattgcgc cgcatgactg
     3661 acaacttcga ccgattttca aattcgcgcc acaagccgag cagaagtcag atccagcaac
     3721 attcgcagcc ccgcaagtcc cacatcgacc cggtaaaggc ggaacatagc cgccagcttt
     3781 gcgtgcgatg tgatcttcga ttatcttcca tgcgtcttca gtgagtctgt attgccagta
     3841 agcgccgagc gcggccagcg caaggagcgg agcaaacacg attgcgccaa cagcagctac
     3901 agcggccttg tcgaaccatt tttgcattcc catttcgact cgagtgccac cagcttctgg
     3961 cgtgatcttg attgtgagtg ccgaagacaa tcctgtcagg tcgcgtaacg tgttcgactt
     4021 gcgcgcttga aagatgtttg ttgacgacac ctgaaacgcc tggacttcaa agtcactgtc
     4081 aaacaaattt cgcaattcag cacttaagtt gggaatatcg acttctacgc ttgggtaata
     4141 tcgactgtcc atctttcgta tcgcagctcc ctttcatgcg gtatccgtca tcattgtacg
     4201 gtattaagct gcgttgtgtt caccgcatgt caattcttaa cttcgccagc ccctttagaa
     4261 aaatgatgcg tagcctaatt acctgtcttc ttttgctgag ttttgcctca agccttgttt
     4321 attcgcaaag cgcacccacc tcacgctcca tcgatggttt ctcgacgcaa accacgattg
     4381 acgaacgaca acttgaagaa aagtttcgtg caattccgaa cccggcctct gctcgcgaac
     4441 atctgcgaca gctgacgatg gagccgcaca tcgctggaag taaagaggac tatgcaacgg
     4501 cgatctacgt tcgggatcag ctgaagagct atggcctttc agcagagttg aaagaatatg
     4561 aggtttggct caactatccc aaaagcccat cggtgctcga gttgatcacc aatcgccggg
     4621 a
//
LOCUS       DASSFP010000257         5440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1555731, whole genome shotgun sequence.
ACCESSION   DASSFP010000257 DASSFP010000000
VERSION     DASSFP010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555731"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VFH91_05640"
     CDS             complement(<1..1293)
                     /locus_tag="VFH91_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HET6862505.1"
                     /translation="MIRIEDIVEKVAANHAQADLDLLRRAYLFSAREHKGQKRASGES
                     YLVHPLEVANILADMKLDEVSVATGLLHDVVEDTLVDLDTIRQYFGEEITHLVDGLTK
                     IAQISNISREEQQAENVRKMLLAMIDDVRVVLVKLADRLHNMRTLQYLNPEKRKRIAE
                     ETMEIFAPIAHRLGMGKLRGELEDLAFRHLHPEDYRELAEQLEKRRPENEAFLKGVTA
                     RIEEKMNEADVPFVTVEGRVKRLYSIWKKLRLQRISLDQVYDLVAARIVTPNDVRYCY
                     GALGVVHNNWKPVPGRIKDWIATPRDNLYQSLHTSVIGSTGQSFEVQIRTEEMHRIAE
                     EGVAAHWKYKEGKRGARDDDQALQALRSLVEWTQEVKDSKDFLDSLKLDLYPKDVYAF
                     TPMGKVIQLPRGATPVDFGYMIHSEVGNTCTGARINGRM"
     gene            complement(1396..2250)
                     /locus_tag="VFH91_05645"
     CDS             complement(1396..2250)
                     /locus_tag="VFH91_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862506.1"
                     /translation="MKLSQTRIAIVLAVLVATSSGCGVINRIRAKNQLNEAARAYREG
                     KFEEAEQHSREAADLDPTNKTAPMFIARTIHAQYRPGVQNPENIAKAQKAIEAYQKIL
                     ENNPKDDEAYKAIAYLYGATKEDDKLRKWIASRAANEATDPEKRAEAYVVLASKDWDC
                     SFKMTELPTNKVTTVDSSNNKATVSYKKPKDQKEFDQAQMCVKQGLAEAENAIKFDPN
                     NESAWSYKTNLLLEATKLAEMDGKADQKAELQKQYNDALKRTTELNAANQKKKEEEAR
                     QSASPPKG"
     gene            complement(2300..2818)
                     /locus_tag="VFH91_05650"
     CDS             complement(2300..2818)
                     /locus_tag="VFH91_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6862507.1"
                     /translation="MSAMDTDAHEKRSTTTAVPYINVTPLIDVLLVLLIIFMVVTPLK
                     PSRFKADIPTQRDPNEDLSKLKPNPLTLVVSVSSDLQIKLNQDAKGSVNDTAVLSQTL
                     AQVFQQRKEQRAYRVGMETRSDLPEDERIEKTVFVKAPRSVKYGDVVKVIDAIKGAGA
                     SPVGLQVDDLPQ"
     gene            complement(2854..3333)
                     /locus_tag="VFH91_05655"
     CDS             complement(2854..3333)
                     /locus_tag="VFH91_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6862508.1"
                     /translation="MPIRKGKVVMGMSGGGGQGGVQSEINVTPMVDIMLVLLIIFMVV
                     TPFLQQGITVNLPKNMNSPDVDQNIIKESSIVISVPNDGEYYLGKKRVQKEELGDKVD
                     SMLKNIKNDQDRIVYIKSGVGVSYGDVVTVINEVRKLGVDKIGLVADKKKKGAPPAA"
     gene            complement(3386..4159)
                     /locus_tag="VFH91_05660"
     CDS             complement(3386..4159)
                     /locus_tag="VFH91_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET6862509.1"
                     /translation="MTILTSILGTKALGLLLLLQEAATSPQASDADHFTLTEMVKNMG
                     GVAIAVVIVLLIMSVYSIAIMVERYLTYSAAKKQSREFAPRVAQALKNDRIEEAINIS
                     DKHRKSHLAMVVSSGLQEFRAHEASSDISGDEIDASKRALQRAIAIKTAEFKRGLSGL
                     ATIGSTAPFVGLFGTVFGIINAFRGMKNAESAGIAAVAGGISEALFTTALGLLVAVPA
                     VWLFNYFTGKVDGFVVEMDNSASELVDYFIKNRTKQLKP"
     gene            complement(4256..5041)
                     /locus_tag="VFH91_05665"
     CDS             complement(4256..5041)
                     /locus_tag="VFH91_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET6862510.1"
                     /translation="MFDNLVESSSHKDDISRKGSFVAATALIYAVLMVAFFVAGIYWY
                     DNHLGQMELELTTLVAPVPVPQQAKPDEPKQEAKPQKVEQNVDVRKELIADVTRTELV
                     PKEVSAKASDIPPVRRGVTTVLGTESSNAQNALPAGPGSGTVTTGPTKVNIADEPPPP
                     APTPTPRTAPISGGVLNGKAISLPKPAYPPIARAAHAAGTVVVQVLIDENGSVVSAKA
                     VSGHPLLQAVAVSAARQARFSPTKLSGQPVKVTGVIQYNFVAQ"
BASE COUNT         1200 a         1428 c         1365 g         1447 t
ORIGIN      
        1 catgcgtcca ttgattctcg cgcccgtaca agtattcccg acttcagagt gaatcatgta
       61 accgaagtca acgggcgtag cccctctcgg cagctgaatc accttcccca tcggagtgaa
      121 ggcataaaca tccttcgggt aaagatcgag cttcaaagaa tcaagaaagt cttttgagtc
      181 ttttacttcc tgtgtccatt caaccagcga tctcaaagcc tgaagggcct ggtcgtcgtc
      241 acgggcgccg cgttttcctt ccttgtattt ccagtgagca gcaacacctt cttccgcgat
      301 gcgatgcatt tcttccgttc gaatctgcac ttcgaacgac tgaccggtcg aaccgatcac
      361 ggacgtgtga agcgactgat agaggttgtc tctgggagtg gcaatccaat ccttaattcg
      421 tcccggaaca ggtttccaat tgttatggac cacgcccagt gcgccataac agtagcgaac
      481 atcgttagga gtcacgattc tagcggcaac cagatcgtag acctggtcca acgagatccg
      541 ctgtagtcga agttttttcc agatcgaata caaacgtttg acgcgacctt cgacagtcac
      601 gaacggcacg tcagcctcgt tcatcttctc ttcgattcga gctgtaaccc cttttaagaa
      661 ggcctcgttc tcgggtcgtc gcttttcaag ttgttcggcg agttcgcgat agtcttccgg
      721 gtgcaggtga cggaacgcaa gatcctcgag ttcgccgcgg agtttaccca ttccgagacg
      781 atgagcaatt ggagcaaaaa tctccatcgt ctcttcggcg atgcgttttc ttttttccgg
      841 gttgagatat tgaagagtcc gcatgttgtg cagacgatca gccaatttga cgagcacgac
      901 tcgcacatcg tcgatcatgg ccaacaacat cttgcggacg ttttctgcct gttgttcttc
      961 ccttgagatg ttgctgatct gggcaatctt ggtgagacca tcaacaaggt gtgtaatctc
     1021 ttcaccaaag tactgtcgaa tggtatcgag atcgacgagg gtatcctcaa ccacgtcatg
     1081 caggaggccg gtagccaccg acacttcgtc cagcttcatg tcagcgagga tattggccac
     1141 ttccagtgga tggacgaggt aagactcacc tgaagcgcgc ttttggcctt tgtgttcgcg
     1201 ggcagaaaaa aggtaggcgc gacgcaaaag atccagatct gcttgagcgt ggttagccgc
     1261 tactttctcg actatgtcct cgatacgaat catcagcgat gccagggctt agtaccggaa
     1321 ttagtataac aaaaaggtga tgtcgcgact cttagagccg gacatcaccc agtgttgaat
     1381 cagacaaact tgagactaac ctttaggagg ggaggctgac tgtcgtgctt cctcttcctt
     1441 ctttttctgg ttagcggcat tgagttcggt ggtgcgtttc agagcatcat tatattgctt
     1501 ctgaagctcg gctttctgat ccgctttgcc gtccatttca gccagcttag tcgcttccag
     1561 aagaaggttt gtcttgtagg accaggctga ttcgttgtta ggatcaaact tgatggcgtt
     1621 ctcagcctcg gctaagccct gttttacgca catctgagct tgatcaaatt ctttctgatc
     1681 ctttggtttc ttgtacgaga cggtggcttt gttgttactt gaatcaacgg ttgtcacttt
     1741 attagtgggc aattccgtca tcttaaacga gcaatcccag tctttactgg cgaggacgac
     1801 gtacgcttcc gctctctttt ctggatcagt ggcttcattt gccgcacgac tcgcaatcca
     1861 ctttctcagt ttgtcgtctt ccttggtggc gccgtaaaga taggcgatag ctttataagc
     1921 ttcgtcatct ttgggattgt tttcgagaat cttctgatag gcttcgattg ctttttgtgc
     1981 cttggcaatg ttctctgggt tctgaacacc agggcgatat tgcgcatgga tcgttctggc
     2041 gataaacatt ggcgccgtct tgttggttgg gtcgaggtct gctgcttctc gcgagtgctg
     2101 ttcagcctcc tcgaatttgc cttcacgata agcgcgcgca gcctcgttca actgattctt
     2161 cgctcgtatt cgatttatta ctccgcaccc ggaactagtg gcaacgagga ccgccaaaac
     2221 gatggcgatc ctggtttgag agagtttcat ttatccatga ctccattaat aaagaccggc
     2281 agtgctggtc tctgtatatt tactgaggaa gatcgtcaac ctgaagacct acggggctcg
     2341 ctccagcgcc cttgatagca tcgattacct taacgacatc tccgtacttc acgcttcgcg
     2401 gcgcctttac gaacacagtt ttctcgattc gctcatcctc cggcaaatca gaacgcgttt
     2461 ccatacctac acgataagca cgttgctctt tgcgttgctg aaagacctgg gccagcgttt
     2521 gcgaaagaac tgccgtgtca ttgaccgacc ctttggcatc ctggttcagt ttgatttgca
     2581 aatcggatga gaccgaaacg accagagtca gtggattagg tttcaactta ctcagatctt
     2641 cgtttggatc tcgctgtgtt ggtatatcgg ctttgaagcg acttggctta aggggcgtaa
     2701 ccaccatgaa gatgattagc agcacaagta atacgtcgat gagcggagtg acattaatat
     2761 acggaacggc cgtagtagtg cttcgcttct catgcgcatc ggtgtccatc gctgacatct
     2821 aaattttctc ctcttgataa aactttgatt tgactaggct gcgggaggtg cgcccttctt
     2881 cttcttatcg gccacgagtc cgattttgtc tacgcccagt ttccggactt cgttgattac
     2941 ggtaactacg tcaccatagc tcacgccgac gccactcttg atatagacaa tgcggtcctg
     3001 atcgttcttg atgtttttca acatcgagtc gactttgtca ccgagttctt ccttttgcac
     3061 tcgcttcttc ccgaggtagt actctccatc attgggaacg gatattacta tcgacgattc
     3121 tttgatgatg ttttgatcga catcgggact gttcatgttc ttcggcaggt tgactgtgat
     3181 gccctgctgc aggaacggcg tcaccaccat aaagatgatg agcaaaacga gcatgatgtc
     3241 gaccatgggt gtgacgttaa tctcggactg tacaccgccc tggccacctc cacctgacat
     3301 tcccataacg actttccctt tccttattgg caattaactc cgcgcggcga ctcagcaccg
     3361 cgcggggtcc accgttttga acacactatg gtttcaactg tttggtgcgg ttcttgatga
     3421 aatagtcgac tagctctgaa gctgagttgt ccatctcgac cacgaaaccg tcaactttgc
     3481 cggtgaagta gttgaagagc cacaccgcag gcaccgcaac caacaaaccg agcgctgtag
     3541 taaagagcgc ttccgagata ccgccggcga ctgccgcgat accagccgat tcggcgttct
     3601 tcataccgcg gaacgcgtta atgatgccga acaccgtgcc gaacagtccc acgaacggag
     3661 cggttgaacc gatggtcgcg aggccggaaa ggccgcgttt gaattcggca gtcttaatcg
     3721 cgattgcgcg ttgcagagcg cgtttcgagg catcgatctc atctcctgag atatcggaag
     3781 atgcttcgtg agcgcgaaac tcctgcaaac cactgctgac caccatggcc aggtgcgact
     3841 tgcgatgctt gtcgctgata ttgatcgctt cttcgatacg atcattcttc aacgcctgag
     3901 ccacgcgtgg tgcaaactca cgcgattgct tcttggccgc cgagtaggtc aagtagcgct
     3961 cgaccatgat cgcgatcgaa tagaccgaca tgataagcag cacgatgacg actgcgattg
     4021 cgacgccacc catgttcttc accatttccg tcagggtgaa gtgatctgcg tccgaggcct
     4081 gcgggctggt ggcagcttcc tgcagcaata gcaagagacc gagggccttt gttcctaaaa
     4141 tgctagttag aattgtcacg gtgaacccct ccaaagagtt ttattagatt gatagcaagc
     4201 agagaaaccg gacggcccac gggaaccgcg gcgaccagac aatacttctc cgcagttatt
     4261 gagccacgaa gttgtactgg atcactccgg taactttaac tggctgtccc gaaagcttag
     4321 ttggggagaa tctcgcttga cgcgcagcgc tcacggccac cgcttgcaag agcggatgac
     4381 ctgagactgc ttttgctgaa accacactgc cattctcatc gatcagcact tgcactacga
     4441 cggtgcctgc cgcatgtgcg gctctggcaa tcggaggata cgctggctta ggtaagctga
     4501 tcgctttacc attcagcacg ccgcccgaaa ttggagcggt cctgggagtc ggcgttggcg
     4561 ctggtggcgg tggttcgtca gctatattga cctttgttgg ccccgtggtg acggtgcccg
     4621 atcctggacc agcaggcagc gcgttctgag cgttgctgct ttccgtacct aatacggtcg
     4681 taactcctcg ccgaactgga ggaatgtcgc tggctttcgc agaaacttct ttcggcacca
     4741 attcggtgcg agtaacgtct gcgattagct ctttacggac gtcaacattt tgctcaacct
     4801 tttgcggctt ggcttcctgc ttaggctcat ctggcttcgc ctgttgcggc accggaacgg
     4861 gggccacgag agttgttagt tccaactcca tctgtccaag gtggttgtcg taccagtaga
     4921 taccagcaac aaagaaagcc accatgagca ctgcgtaaat caatgctgtg gcggcgacaa
     4981 atgagccttt tcgcgatatg tcgtccttgt gcgaactgga ctcaacaaga ttatcgaaca
     5041 tttatatttg tcctccaatt ccggcgccaa atgggatcag gggttgcgaa attgtagtag
     5101 ctaactctca aaggtcgacg tctgggctac tttgagtttc cgtcagtata gaaaggggaa
     5161 aacagcagct cctgcgatat ttgagcacac cgaatagact tgtgaagccg ctgtgcgaga
     5221 aaaactcgcc ggcctgctca acatactacc gccgcgccca acttctaatt caaacgcgca
     5281 gggtcgactt tgagatattt aattggccag caacggtcgc agtctattcc gcatggcgtt
     5341 tcaaagtcaa gcgcaaactc gacttgatct gcttttcaaa cgcaaccgat catctgcaaa
     5401 cgaaaactta cgcgcgtcca gggcgactgg attgtttgtt
//
LOCUS       DASSFP010000258         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2152219, whole genome shotgun sequence.
ACCESSION   DASSFP010000258 DASSFP010000000
VERSION     DASSFP010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152219"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFH91_05670"
     CDS             <1..379
                     /locus_tag="VFH91_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862511.1"
                     /translation="GLSPGQVQARLARYAKGIGTITDLRVVSRGSSHRVTDLELIGTS
                     GTSHVRGGRIRSALGLREQLFVIDREYDESGRITEFVFTGRGWGHGVGMCQVGAYGLA
                     KQGYTFDQILKAYYTGIEITKMY"
     gene            357..1442
                     /gene="miaA"
                     /locus_tag="VFH91_05675"
     CDS             357..1442
                     /gene="miaA"
                     /locus_tag="VFH91_05675"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET6862512.1"
                     /translation="MRLRRCTEAGSFGNDLFTKSSRRRFADTRPHLDSHPLPRDILWF
                     VTADSDNSPVIAIVGPTASGKSTVAIETALLLNGEIINCDSVQVYQQIKIASAKVPIE
                     ERKGVPHHLIDFVSPNINYTAGEWARDAAREIEAIEGRGRIPLLVGGTGFYLRALRQP
                     FFSSPDTNSSLRQRINNIRKRHGPEHVHKLLQRLDPKAAKEFFPRDWPRVQRALEVYL
                     QTGKSLVDQKSVRPDAHESARRLRILALNPPRADLYSRIDARTDEHFKAGLVEEVRDL
                     LARGFPAESNALGAHGYRRVVEYLQGKRDLPSAIAQTKLDIRHYAKRQLTWFRHEPGV
                     EWIYGFGEKKAVLDSIIEKLVTGPTTK"
     gene            1548..1850
                     /gene="hfq"
                     /locus_tag="VFH91_05680"
     CDS             1548..1850
                     /gene="hfq"
                     /locus_tag="VFH91_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET6862513.1"
                     /translation="MSDGKQAPQNIQDAFLNTVRREKTTVIVYLLNGAKLTGRIRSFD
                     KFSVLLETGTQEQLIFKHAISTISQSRRNTGDLHSQPVAEAETRESRSAEASSPEA"
     gene            1898..2587
                     /gene="tmk"
                     /locus_tag="VFH91_05685"
     CDS             1898..2587
                     /gene="tmk"
                     /locus_tag="VFH91_05685"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET6862514.1"
                     /translation="MSGTFITFEGIDGSGKSTQLRLLNNFLRENGSQVVTTREPGGTT
                     LGLRLRAALLDATEEVDPLTELLVFAADRAQHVRRLLRPALAAGSVVISDRYADATVA
                     YQGAGRGFSPELISDIVKLATDGLKPDLTLLFDVSVDESTTRTTRRVSGRGGSGKNQR
                     DRLDKEAVDFHARVREAYLAIAKQEPERVKIIDTSGPVESTHERVKQILVPFLQSRGH
                     LMHLQVELEKA"
     gene            2588..>2886
                     /locus_tag="VFH91_05690"
     CDS             2588..>2886
                     /locus_tag="VFH91_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862515.1"
                     /translation="MFSELVGNDRVKTALKKMLVSERLPGALLFTGEEGIGKKLFALE
                     IARALNCRTPVDQEGCGKCGSCTRIEKINYPSPSEAENWLQIIWTDHPDVGIVVPP"
BASE COUNT          713 a          723 c          755 g          695 t
ORIGIN      
        1 cggcttatcg ccgggacaag tgcaggctcg attggcccgg tatgccaaag gaatcggaac
       61 gattacagat ctgcgagtcg tttcgcgggg cagttctcac agggtgactg atctggaact
      121 aatcgggacg agtggaacgt cacatgttcg cggtggacga atcaggtcgg cgctcggttt
      181 acgagagcaa ctatttgtca tcgatcgtga atatgatgag agtggacgca tcaccgagtt
      241 cgtatttaca ggtcggggct ggggacatgg agttgggatg tgtcaggttg gcgcttatgg
      301 attagcgaag caaggttaca cgtttgatca gatactcaag gcttattaca cggggattga
      361 gattacgaag atgtactgaa gcagggtctt tcggtaatga ccttttcacg aaatcaagtc
      421 ggaggcggtt tgctgatact cgccctcatc tggatagtca tcctctgccg cgtgatattc
      481 tctggttcgt gactgctgat tcagacaact cacctgtaat agccatcgtt ggaccaactg
      541 cttcaggaaa aagcaccgta gcgattgaaa cggctctcct tttgaatggc gagatcatca
      601 attgcgactc tgttcaggtc tatcaacaaa ttaaaattgc gtctgccaaa gtcccaatcg
      661 aagaacgcaa aggcgttccg catcatctga ttgattttgt ctcgcctaac ataaactaca
      721 ccgccggtga atgggcacgt gatgctgccc gagaaattga agccatcgaa ggtcgaggcc
      781 ggattcctct cctggttggc ggcaccggat tctacttacg tgctttacgc cagccatttt
      841 tctcgagccc agacactaat tcgtcgttgc gccaaaggat caataacatt cgcaagaggc
      901 atgggccgga gcacgtacac aaactgctgc aacggcttga tccaaaggca gcaaaagaat
      961 tctttccacg cgactggcct cgcgtacaac gagcccttga ggtctacctt caaaccggca
     1021 aatctctcgt ggaccagaaa tctgtgcggc ccgacgctca cgaaagtgct cgccgtttga
     1081 ggattctagc gctgaatccg ccccgagccg atctctacag ccggatcgat gcgcgaaccg
     1141 acgagcattt caaggccggt cttgttgagg aggttaggga cctgctcgct cgtggctttc
     1201 cggccgagtc gaacgccctg ggcgcgcacg gctaccgtcg tgtggtcgag tatctacagg
     1261 ggaaaaggga cctgcccagc gccattgcgc agactaagtt ggatattcgg cattacgcga
     1321 agcgtcaatt gacatggttt cgccatgaac caggtgtgga atggatttat ggatttggtg
     1381 agaaaaaggc tgtccttgat tcgattattg aaaaacttgt gactgggcca acgaccaaat
     1441 aatcatgttt ctgtaacatc gcaacctatc tcttgtcagt cttttttcac tgttgtaagc
     1501 tgacacttcc gcgcgtaaag tcatttccgg gtagatccag gttggcaatg tcagacggta
     1561 aacaagcacc acaaaatatt caggacgcct ttctcaacac agtccggcgc gaaaagacca
     1621 ctgtgatcgt ttatctgctg aacggcgcta agctaaccgg ccgcatccgc agcttcgaca
     1681 agttttcggt tttactggaa accggtactc aagaacaact tatttttaag catgcaatct
     1741 ctaccatttc gcagtcccga cggaatactg gagacttgca ctcgcagccg gtggcagaag
     1801 ctgaaactcg tgaatcacgt tcagctgaag caagctcgcc tgaagcgtaa cattcgttta
     1861 aactctccga ataaaatcca atcatttaag aggacgtgtg tctggaactt tcattacatt
     1921 tgagggtatt gacggcagtg gcaagtcgac ccaattgcgt ttgcttaaca attttcttcg
     1981 tgaaaatggg tcccaggtgg ttacaacccg agaacctggt gggacaaccc ttggactgcg
     2041 cctccgtgca gctctccttg atgcaacaga ggaagtagat ccgttaactg agctccttgt
     2101 tttcgcagca gatcgggccc aacacgtacg acgcctgctt cgaccagccc tggcagccgg
     2161 aagtgtcgtg atttccgatc gttacgccga tgccaccgtc gcctatcagg gcgctggacg
     2221 tggtttttca cctgaattga tttccgacat cgtcaagctt gccactgacg gattaaagcc
     2281 tgatctaaca ctgctttttg acgtttctgt cgatgagtca actacccgaa ctacgcggcg
     2341 ggtctcgggc cgaggaggca gtggcaagaa tcaacgagac cggctcgata aagaagctgt
     2401 cgattttcat gcccgcgttc gtgaggctta cctggcaatc gcaaaacagg agccagaacg
     2461 agtcaaaatc atagatacgt ctggaccagt tgaatccact cacgaacggg tgaaacagat
     2521 attagtcccg ttccttcaaa gccgaggcca tctaatgcac cttcaggtag aactggaaaa
     2581 agcctagatg ttcagcgaat tggtcggaaa tgatcgcgtg aaaaccgctc ttaagaagat
     2641 gctcgtctcg gagcggctgc ccggagcgct tctcttcacg ggagaagaag gtatcgggaa
     2701 gaaactattt gcgcttgaaa ttgctcgggc actcaattgt cgtaccccag tcgatcaaga
     2761 gggatgcggg aagtgtggtt catgtactcg gattgagaag atcaattacc cgtctccttc
     2821 agaggccgag aattggctcc agatcatatg gaccgaccat cctgacgtcg gaatcgttgt
     2881 gcctcc
//
LOCUS       DASSFP010000259         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1842949, whole genome shotgun sequence.
ACCESSION   DASSFP010000259 DASSFP010000000
VERSION     DASSFP010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842949"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFH91_05695"
     CDS             <1..990
                     /locus_tag="VFH91_05695"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6862516.1"
                     /translation="RCFNCDRLSFCMAFVIVVVTSLISATAATPSQAQQPSAPANAEN
                     KNDQKPAEPADGSVQGAVPADHYDPDDNKTFPDVRTENSAVTIQGHQFIVHMYRPKKD
                     GPATPAIYACGDGGWRGLAPRTAQQLAHIGFAVAGIDSKIYLREFSSPTSPLTIKQLA
                     SDYSVIAASLRDYAQAPAQTPVYVYGWSLGAGFAIAVGTDQTTRSNWAGIVSIGLPKQ
                     NQLVSGLGANHANLKAEVNSLYGFRSQDLIGWIAPLPLVMIQSTSDTASPPKVGQALY
                     AAARDPKLFVLIKASNHRFSGARDEFYTALSNAESWIRSSETGVSTVRFKDTK"
     gene            1101..1982
                     /locus_tag="VFH91_05700"
     CDS             1101..1982
                     /locus_tag="VFH91_05700"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HET6862517.1"
                     /translation="MIRTLPLDPRSKWAWLIGVVFLLFGSATVYAVVWYRHFYHHVTV
                     QELSHEVQHSTFQTTIRGGSPLTLQVYQQSNASNQPLVLFTSGDGGWSPFCADIAAHI
                     AATGRTVVGFNMKDYLVTFASSQKPVTTDELTGDYEAIIDVSVARPGVDHGTKATLAG
                     WSVGAGYSVMMAVEPRLRDRVSQVVAISLPIYNELAWKPTDALIYFTHSTPHEKVFDT
                     RKYLPELGNTPISMLNATNDDTSPYKEAQSLAAAVKGPKQFYTIQATGHHFEGGEDEL
                     YRCLDQALAGEPALTKQ"
     gene            complement(1994..2194)
                     /locus_tag="VFH91_05705"
     CDS             complement(1994..2194)
                     /locus_tag="VFH91_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862518.1"
                     /translation="MMVTIDRALKKFFHILAKGFSGLHWAIGITALPENASSSEERSF
                     VLMWLGLIVFVVAVFAMMLYLL"
     gene            complement(2197..2928)
                     /locus_tag="VFH91_05710"
     CDS             complement(2197..2928)
                     /locus_tag="VFH91_05710"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET6862519.1"
                     /translation="MTITTACGPRSRAQPVNDAATAKAEVAQADQMYAQRADLRQLRQ
                     AIVLLEQATITDPDNYDAAWRLSKFNYYLATHSTDKHERDKAFSDGIVAGKAAVQLQN
                     GKPDGHFWLGANYGGTAQSSILAGLSSVGEIRSEMETVIRQDPGYQNGSAYMVLGLVY
                     LNAPGFAGGDPKKAVEEMEKGLPFGGENAFLRLHLAEAYIKVGRKMDARRQLEAIINM
                     TPDKDYVPEYKESVTAAQKLLQNTT"
     gene            3189..4766
                     /locus_tag="VFH91_05715"
     CDS             3189..4766
                     /locus_tag="VFH91_05715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6862520.1"
                     /translation="MRKYTACFVFSLSLVLVQVVPSYNLLYGRSDRAAATTQKTFSLP
                     RSSPEQQGISSSDLLAFVEAADKEIDAMNSLMIVRHGHVIAEGWWGPYDRDTPHILYS
                     LSKSFTSTAVGLAISEGKLSLDDQVLKFFPEDAPADPSANLRAMRVRDLLRMSSGNQT
                     EALIRAVDSGSGTDSWTKIFLAHPVPFKPGTHFLYNSPASYMLSAIVQKVTGMTVLEY
                     LRPRLFDPLGFKDPRWISSPQGITAGAYGLSARTEELASFGVLYLQKGMWNGKQLIPA
                     AWVEQATSMQTSNGSAPTSDWDQGYGYQFWRSRHHSFRGDGAFGQYCMVIPELDTVVA
                     MTSGVRDMQKVMNLVWDKLLPAMKDGRLPENATTRKQLESRLAGLTVKFPNGLSSSRS
                     ALSISGKWFELPENERGLKAISFDFNSATPMFRVQTAAGETRTPLGFNTWIKSRGSFT
                     NGLDRFLSVPDNPLIAASGAWTSEDTYTLRIVLYETPFYSTTTFKFDGDRVLIDSEHN
                     VAFGPTKLPQLTGHLRT"
BASE COUNT         1242 a         1271 c         1215 g         1246 t
ORIGIN      
        1 cgctgcttca attgcgatcg cctttctttc tgcatggctt tcgtcatcgt cgttgtgacg
       61 agtttgatct cggcgacagc cgcaacgcca tcgcaagcac aacaaccttc cgcgccggcc
      121 aacgccgaga ataagaacga ccagaagccc gccgaaccgg cagacggaag cgttcagggc
      181 gctgttccag ccgatcatta cgatcctgat gacaataaaa cattccctga cgttcgaact
      241 gaaaacagtg ccgttacgat tcaaggtcat caattcatcg ttcacatgta ccgtcccaaa
      301 aaggacgggc ccgcaactcc tgcgatctat gcttgcggag atggaggttg gcgaggtctc
      361 gcgccacgga cggcccaaca gttggcccac atcggctttg ccgttgctgg aatcgattcc
      421 aagatctatt tgagagaatt ctcatcgcca acgtcaccac tcactatcaa acaactggca
      481 agtgattatt ctgttatcgc ggccagtctt cgtgactacg cacaagcgcc ggctcaaacc
      541 ccagtttacg tctacggatg gtcgctcggc gccggctttg caatcgctgt tggcacggat
      601 cagacgactc gctcaaattg ggccggcatc gtctcaattg gtttgccaaa gcagaatcaa
      661 ctggtcagcg gactgggcgc aaatcatgca aatctgaagg cggaagttaa ctctctttac
      721 ggtttccgca gtcaggatct gatcggatgg attgcaccgc tgcctttagt aatgatccag
      781 agtacttctg acacagccag tccgcccaag gtgggacagg ctctttatgc tgcggctcga
      841 gatccgaagt tgtttgtatt aattaaggcg agcaatcacc ggttcagtgg agctcgtgat
      901 gagttctaca cggcgttgag caatgctgag tcatggatac gctcgtcgga aacgggggtg
      961 agtactgtca gatttaaaga taccaagtag tgaaattggc ggagaaatta tcatttaaga
     1021 attatcaatt gagaattgac ttcatggacg acttgtgtgg ccaaaacagt aattatcaaa
     1081 tctcaaataa caattgacca atgattagaa ctttacccct agatcctcga agcaagtggg
     1141 cctggcttat cggagtggtc ttcctgctgt ttggttcggc aactgtttat gcagtcgttt
     1201 ggtatcgcca cttctatcac catgtaacgg tccaggaact atcacatgaa gtgcagcact
     1261 ccacttttca aactacaatt cgtggtggct ctcccttaac tcttcaggtc tatcaacaat
     1321 ccaacgcgtc aaatcaaccg cttgtgcttt ttacgagtgg cgatggtggc tggagtccat
     1381 tttgcgcgga catcgctgct cacattgcag ctacgggacg aacagtcgtt ggatttaaca
     1441 tgaaagacta tctcgtcacg tttgccagtt ctcaaaaacc tgttacaacc gatgagctga
     1501 caggcgatta tgaagcaata attgatgttt ccgtcgcacg accgggtgtg gatcatggaa
     1561 ccaaagcaac attagcagga tggtcggtcg gtgccggtta cagtgtgatg atggccgtcg
     1621 agcctcgttt aagagatcga gtgtcccagg ttgtcgctat tagtctgccg atctacaacg
     1681 agctggcatg gaaaccaact gacgcgttga tctatttcac gcattccacg ccgcacgaga
     1741 aggtatttga cacgcgaaag tatttaccag agttaggtaa tactcccatt tcaatgctca
     1801 atgcgaccaa cgacgatact tctccttaca aggaggctca gtcgctcgca gctgcggtta
     1861 agggaccaaa gcagttctac acgatccagg ctacgggaca tcactttgag gggggagagg
     1921 atgagcttta ccgctgcttg gaccaggcac ttgcaggaga gcccgcattg acgaagcaat
     1981 agatcgaact aactcacaat agataaagca tcatggcgaa gactgccacg acaaaaacaa
     2041 tcagacctag ccacatcagc acaaatgagc gctcctcgct ggaactggca ttctctggaa
     2101 gcgccgtaat tccaattgcc cagtgcaggc cactaaaacc cttcgccaaa atgtgaaaga
     2161 atttcttcag tgctcggtca atagtcacca tcatcatcaa gtggtgttct gcagcagctt
     2221 ctgggccgca gtcaccgact ctttgtattc agggacatag tctttgtcag gcgtcatgtt
     2281 gataattgcc tcaagctgac ggcgcgcgtc catcttacgt ccaactttta tataagcttc
     2341 agcgagatgc aagcgcagaa acgcattctc ccctccaaac ggcaaacctt tttccatctc
     2401 ctccactgct ttcttaggat ctcctccggc aaaccccggc gcatttagat agaccaaacc
     2461 aagcaccatg tacgcactac cattctgata gccaggatcc tgtcgaatca ctgtttccat
     2521 ttcactgcga atctcgccta ctgaagaaag accggccagt atactggact gagcggtgcc
     2581 gccataattt gcgcctaacc agaagtgacc atcaggtttt ccgttctgca gttgaacggc
     2641 agctttacca gccacaatgc cgtcgctaaa agccttgtct cgttcatgct tatcggtgct
     2701 gtgtgtggcc agatagtaat tgaacttaga aagccgccag gctgcatcgt agttgtccgg
     2761 atcggtaatt gtagcctgtt caagaagaac tatcgcttga cgcagttgcc tcaagtccgc
     2821 gcgttgagca tacatctgat ctgcttgcgc gacctcagct ttagcagtcg cagcgtcgtt
     2881 aacgggctgc gctcttgagc gcggtccaca agcagtcgta atggtcatca acgtaaccat
     2941 accaacgaga ataagatttc gattatagag aagtttatta atcgtctttc acctttcacc
     3001 ctttttggtg agttatagat gagagccggt tgtgagtcgt tcgccggttt gccacgaatt
     3061 aaactatttt gctggtttct gtttaaccag actaagagac gatcgtatga cacctgtcta
     3121 tgcgtataat ttatccggtt tgctaaagtt tgaggctcgc gtccaatctc catattgaga
     3181 gaggtatcat gaggaaatat acagcgtgtt ttgtcttctc tttatcattg gtcctggtcc
     3241 aggtcgtccc atcgtacaat ctcctctacg gccgtagcga cagagcggct gcaacgactc
     3301 aaaagacctt ttcgttacct cgtagcagtc ccgaacagca aggtatctca tcctccgacc
     3361 ttcttgcgtt cgtcgaagca gccgacaaag aaatagatgc aatgaacagt ttaatgattg
     3421 tcagacatgg acatgtgata gccgaaggct ggtggggtcc ttacgaccgt gatacaccac
     3481 acatcttgta ttcgttgagc aagagtttta catccactgc cgtcggactt gcgatctctg
     3541 aaggtaaact cagcctcgac gatcaagtac ttaaattctt tccagaagac gctccagctg
     3601 acccttccgc caacttgcgt gcaatgcgag tgcgagattt gctgcgaatg tcctcaggga
     3661 atcagacgga agcgttgatt cgggccgtcg attcggggag tggaactgat tcatggacca
     3721 agatttttct tgcccatccg gtacccttca aacccggcac ccactttctc tataactccc
     3781 ccgcctcgta catgttgtcg gcgatcgtcc aaaaggttac gggcatgacg gtgcttgagt
     3841 atctacgacc aagactcttc gatccgctgg gctttaagga cccccgatgg attagcagtc
     3901 cacagggaat aactgctggt gcttacggct tgtcggcgcg tactgaggag ctagcgagct
     3961 ttggagttct atatctgcaa aaaggaatgt ggaacggcaa gcagttgata ccggcggcat
     4021 gggttgagca ggcgacctca atgcagacgt caaatggttc cgcgccaacc agcgactggg
     4081 atcaggggta tggctaccaa ttctggcgtt cccggcatca ctcttttcgc ggagacggag
     4141 cattcgggca gtactgcatg gtcattcctg aacttgatac cgtggtcgca atgacgagtg
     4201 gcgttcggga catgcaaaaa gttatgaatc ttgtttggga caaacttttg cccgctatga
     4261 aagacgggcg attgcctgag aatgcgacta ctcggaaaca actcgaaagc cgcctggccg
     4321 ggttgacagt taagtttcca aacggattgt cgtcatctcg ttcagcgttg agcatatccg
     4381 gcaagtggtt tgaactcccc gaaaatgaac gtgggctaaa ggcaatctca tttgatttta
     4441 attccgctac acctatgttt agggttcaaa cagcagcggg cgagacacga acaccgctcg
     4501 gttttaatac ctggatcaag agtcgaggtt cgttcacaaa tgggctggat cgatttctta
     4561 gcgttccaga taatccgcta atcgccgcga gtggagcctg gacctccgaa gatacctaca
     4621 cattaaggat tgtcctttat gagacacctt tttactcgac gacaactttc aagttcgatg
     4681 gagatcgagt acttatcgac tccgaacata acgttgcgtt tggcccaaca aaactcccac
     4741 agttaaccgg tcacctccgc acctaatttt ggagtgcgcc ggcccggcgg cgctttgtta
     4801 tatcgggcca tttgttaaac gagactttag caaggcagtt gcatttaaag cgccgccggg
     4861 ccggcgcact ccaaaggtcg ctcggatgat agggagttcc ccaccaaaaa ttgtgtggac
     4921 gtaatcgaga ctctaatacg cgtatataaa caggagatgt tgaacgaagc taaa
//
LOCUS       DASSFP010000260         6846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_69008, whole genome shotgun sequence.
ACCESSION   DASSFP010000260 DASSFP010000000
VERSION     DASSFP010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69008"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFH91_05720"
     CDS             <1..232
                     /locus_tag="VFH91_05720"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HET6862521.1"
                     /translation="ASAATDAQLKAEILTYSRSRGLFAGVSLEGATLRTDDDANKDLY
                     GKSTSNKDIVSGAVATPASATALMSEVKKHAG"
     gene            631..2394
                     /locus_tag="VFH91_05725"
     CDS             631..2394
                     /locus_tag="VFH91_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET6862522.1"
                     /translation="MKRLHVSEQGSEETRFETGMRRLTDTNLLGLLFIDAAGAIRKAD
                     ESFLNTVGYTESDLPETLYELSVPEDHRLVEEAFEKLMAFGACAPFEHELVRKDGTHV
                     PVLFGAALQEEEIVCFIVDLSQTKLATERLNHLAYHDALTDLPNQALFKDRLKQAIAI
                     SRRTEQMQAVLLLNLDRFKTINDSLGYNAGDRVLQSVAKRLTSCLRESDTVSRFGSDE
                     FAILLTQIRPTDAANTARAIKDVLDQAFVFDDGQELFVSSSIGISLYPYDGQDTPTLL
                     KSAGAALDRAKAQGGNNYQFYTAGGTTRALRQLVLESSIRPGLDRGEFIVHYQPQVNT
                     SDFQLVGMEALVRWQHPALGLLYPSEFISLAEDSGLIIPLGDWVLRTACFQNKLWQVA
                     GLKPQVLSVNFSARQFQLPTFIQTVAEILKETNLDPKWLELELTESSIMKDPDQAIEK
                     LHELKLMGIKVAIDDFGTGYSSLNYLKRFPIDTLKIDKSFVSDICKDPHDTAIVRAIV
                     TLGHALDLTVVAEGVETREQLEYLNELECDVVQGFLFSKSLSGDDFEDLLLEQHRVTS
                     SSDYAVDLTDFAAQNIPITTH"
     gene            complement(2460..4013)
                     /gene="zwf"
                     /locus_tag="VFH91_05730"
     CDS             complement(2460..4013)
                     /gene="zwf"
                     /locus_tag="VFH91_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6862523.1"
                     /translation="MSTLLEEPQVEQLGLPTLPKAEPCVIVIFGATGDLTKRKLMPAL
                     FRLMCEASLTRFQILGVGRSAMNDEEFRQMMREGATDKKNQFPDAQWQEFAESLFYVH
                     GEFDDDSTYRNVAGQIEDLSARCGSSNRLFYVATPPSLFGTIVAKLGKAGLAAQDKGW
                     TRLVIEKPFGRDVDTAKKLNAEIGEVFSEDQVYRIDHYLGKDTVQNILVFRFGNTMFE
                     PIWNRNYVDYVEITAAEKLGIGSRAGYYEEAGALRDMVANHLLQLLTLTAMEPPVAFD
                     ADSVREGKVQVLRSIRRLKPEEVAERTVRAQYGPGIIDGEKVKGYREENGVANNSVTE
                     TYVAIVFFISNWRWAGVPFYVRTGKHLSRNLTEIAIHLKRTPQALFARTPDEQIEPNI
                     IALRIQPNEGITVTIGAKQPGFEMVTNTVHMDFCYQSGTTVRSPEAYETLLLDVMTGD
                     ATLFIRRDEVEAQWRLITPIEETWAQQKPLELPTYASGTDGPPQADELLARNGHKWRK
                     LNKTVAGCD"
     gene            complement(4044..5063)
                     /gene="gnd"
                     /locus_tag="VFH91_05735"
     CDS             complement(4044..5063)
                     /gene="gnd"
                     /locus_tag="VFH91_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET6862524.1"
                     /translation="MQIGMIGLGRMGANMVKRLTNGGHQCVVYDRNTTAVEDLAKEGA
                     VGSNSLSEFIEKLQPPRPIWLMIPAAFVDSSINELLALIQKDDILIDGGNSYYIDDIR
                     RANELRAKGIHYVDVGTSGGVWGLERGYCQMIGGENEIVAHLDPIFKTLAPGRDVVSS
                     TPGRSGNSTAEQGYLHCGPSGAGHFVKMIHNGIEYGLMAAYAEGLNVLHHANIGKLDH
                     AKDAETTPLRNPDHYQYDFNLADITELWRRGSVVASWLLDLTAGALAEQPQLEKFSGR
                     VSDSGEGRWTIQAAIEEGVPAPVLSAALYQRFSSRGEGDFGNKVLSALRFQFGGHVEK
                     TTDGK"
     gene            5226..6461
                     /locus_tag="VFH91_05740"
     CDS             5226..6461
                     /locus_tag="VFH91_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6862525.1"
                     /translation="MESTASVSVEIDQSRRWQVLGLLAIAELLAMSLWFSVSAVVPSL
                     RAEWNLSEAAAGWLTVAVQLGFVFGTLLSAILNLADIVRARHLVAITAFLGAFTNASF
                     ALFAHDALMAIALRFLTGVFLAGVYPPGMKVMASWFRRSRGLALGVLVGALTLGKASP
                     YLINAIGSSNWRYNVLVVSTLAAIGGLIVLLFVSDGPYGLPRAQFDWRQVLKIFRNRP
                     VLLANFGYFGHMWELYAMWTWIPVMLRSSMVLSRKDGVWAEVGSFVVIGSGAAGCVLA
                     GLIADRVGRTMVTSWAMIISGSCCLIVGFLYGGDPILLLIVSAIWGASVVADSAQFSS
                     CVTEVGDPQYIGTTLTLQMCIGFLLTTVSIELIPRMVNLVTWRYAFAILAVGPALGVV
                     SMLRLRQLPEAVKIANGRR"
BASE COUNT         1560 a         1759 c         1710 g         1817 t
ORIGIN      
        1 cgcctcggcg gcaaccgatg ctcagttgaa ggcagaaata cttacatact cgcgttcacg
       61 agggttgttt gctggagttt cacttgaagg cgccacattg cgtactgacg atgacgcaaa
      121 taaggatttg tatgggaagt cgacatcaaa caaggatatc gtgagtggtg cagtcgcaac
      181 gcccgcgtcg gctacggcgt tgatgtcaga ggtaaagaaa cacgcaggct gaaaaggccc
      241 gctttcaatt tcaggtgggg cttgagcgaa gcctggctgc gctcaagccc aaaccgttcc
      301 cgccttcgct caacactcca agaactcggt tcttagcttc acgcgcctcc tcgttccgaa
      361 taaaggccca ctggtccgtt cgtttcgtca gttggggacg gcttaagttg cgaaaaatag
      421 aggaatttcg atagcttgca ttaccttttt ctattcttcg ggtttggcgc ccgcgagaaa
      481 aattatttca ttgtaaagag gtctgcttct ctgttagcct aagaacggtt ggtcgcggct
      541 ttcccaacct ttctgccctt cctctcattt tttaactgac agttttactt ccagttcccg
      601 cattctcatc acatgaccta gggatttgcg atgaaacggc tccacgtctc cgaacaaggg
      661 tcggaagaaa ccagattcga aacaggtatg cgacggctca ccgacacaaa cctacttggt
      721 ttgttgttta ttgatgccgc cggtgccata cgtaaggccg atgagagttt tctgaatacg
      781 gtcggctaca ccgaaagcga ccttccggaa acgctctacg aactctcggt tcctgaagat
      841 caccggctcg ttgaagaagc tttcgaaaaa ttgatggcat ttggagcatg cgcgcctttt
      901 gagcacgaac ttgttcgcaa ggacggaaca catgtcccgg tcctatttgg cgcggcactc
      961 caggaagaag agattgtttg tttcatcgtc gatctcagcc aaacaaagct ggcaacggaa
     1021 cgactcaatc atctggctta tcacgacgcg ctcaccgatt tgcccaatca ggcactcttc
     1081 aaagatcgtc taaaacaggc cattgcaatt tcgcgccgca ccgagcagat gcaggcagtt
     1141 ctgttattga accttgatcg tttcaagact attaacgact cgctcggata caacgcgggt
     1201 gatcgagtac tacaatcggt agcaaaacgc ctcacgtcat gtttacgaga gagcgatacg
     1261 gtttcgcgct tcggttcgga tgaatttgca atcctgctaa ctcagatcag acccacagat
     1321 gctgcaaaca ctgcgcgggc aatcaaggac gtattagatc aggcatttgt ctttgatgat
     1381 ggccaggagt tgtttgtatc aagcagcata ggaataagcc tctatcccta cgacggccag
     1441 gacaccccga cactcctcaa gagcgcgggt gcggcactgg atcgagccaa ggctcaaggc
     1501 ggaaacaact accagttcta taccgccggt ggaacgacca gggcgttacg tcaactcgtg
     1561 ctggagagca gtattagacc aggactggat cgaggcgagt tcattgttca ttatcaaccg
     1621 caggtaaaca cttcagactt tcagctggta ggcatggagg ctttagtgcg atggcaacat
     1681 ccggctttag gattgttgta cccttcggag ttcatcagtc tggctgaaga tagcggccta
     1741 ataattcccc tcggtgactg ggttttgcga acagcctgtt ttcaaaacaa attgtggcaa
     1801 gtcgctggtt taaaaccgca ggtcctatca gtcaactttt ccgcgcgaca atttcagttg
     1861 ccgactttca tccagacagt tgctgaaata ttgaaggaga caaaccttga tcccaaatgg
     1921 ctcgagctgg agttaaccga aagttcgatc atgaaagacc cggatcaagc catcgaaaaa
     1981 ctccacgagt taaaattaat gggaatcaaa gtggcaatcg acgactttgg caccggatat
     2041 tcgtcactca actacctcaa acgctttcca atcgacactc tcaagatcga caagtcattt
     2101 gtcagtgaca tctgcaaaga tccgcatgat acggccatcg tgcgagcaat tgttacgttg
     2161 ggccatgcgc tggatttgac ggtggttgct gaaggcgttg aaacacgtga acaactcgag
     2221 tatttgaatg agctcgaatg tgatgtcgtc caggggtttc tattcagcaa gtcgttatcc
     2281 ggcgatgact ttgaggactt gttgcttgag caacatcgcg tgacttcgag ttcagactat
     2341 gcagtcgacc ttaccgactt cgctgcacag aacattccca tcacgactca ctaatccatt
     2401 tcgagcaagc tcgccgcctg ataggtgtga cactcgtttt ttatccgcaa gcgaaagctt
     2461 cagtcgcaac ctgcgacagt tttatttaat ttgcgccatt tgtgtccatt tcgcgcgagt
     2521 aattcatccg cttgaggtgg tccatccgtt ccggaggcat aagttggcaa ctccaacggt
     2581 ttttgttggg cccacgtttc ctcgattgga gtgatcaatc tccactgagc ctcaacctcg
     2641 tcgcgacgaa tgaacaaagt ggcatctccc gtcatgacat ccagcagtag agtttcatag
     2701 gcttccggtg accgaactgt ggttccggat tgataacaaa aatccatatg cacagtgtta
     2761 gtaaccattt cgaagccggg ttgttttgca ccgatcgtga cagtaattcc ctcgttaggc
     2821 tgaatgcgca acgcgataat attcggttcg atctgttcat ccggagttct cgcaaaaaga
     2881 gcctgcggcg tgcgctttag atggatggcg atttcggtga gattccgact tagatgttta
     2941 ccggtccgta cataaaacgg cacacccgcc caacgccagt ttgagataaa gaacacgatt
     3001 gcgacgtatg tttccgttac cgagttgttg gctactccat tttcctctct gtaacctttg
     3061 accttttcgc cgtcgattat tcccgggcca tattgtgcac gcactgttcg ttcggctacc
     3121 tcttctggtt taagccggcg gattgaacga agcacctgga cctttccctc cctgacggaa
     3181 tcggcatcga aggcgactgg tggctccatt gcagtcaagg ttagaagttg aagcaagtga
     3241 ttcgccacca tgtcacgaag ggctccagct tcttcgtagt atccagcacg tgaaccaatg
     3301 ccgagttttt cggctgctgt tatctcgacg tagtcaacat aattacgatt ccaaattggt
     3361 tcaaacatag tgttgccaaa acgaaagacc aaaatgttct gaactgtatc ctttccgaga
     3421 tagtgatcga tccgatacac ctggtcctcg gaaaaaacct cgccgatctc agcattgagc
     3481 tttttggctg tgtctacgtc ccgaccaaat ggtttctcga taacaagtct ggtccaacct
     3541 ttatcttgtg ccgcaagacc agcttttccg agtttcgcta caattgttcc aaacaacgac
     3601 ggtggcgtcg ccacataaaa gagtcgattt gaactgccgc aacgcgcaga cagatcttcg
     3661 atctgccccg cgacgttacg ataggttgaa tcatcgtcga actcaccgtg tacatagaac
     3721 aggctttccg caaattcttg ccactgagcg tccggaaatt gattcttctt atcggtggcg
     3781 ccttcgcgca tcatttgacg aaattcttcg tcattcattg cggatctgcc gacgccgaga
     3841 atctgaaacc ttgtaaggct ggcctcacac atgaggcgaa acaaggccgg catcagtttt
     3901 ctcttcgtaa gatctccggt ggccccaaaa atgacaatta cacacggttc agcctttggg
     3961 agggttggca gtccgagttg ctccacttgt ggttcttcta ataatgttga cacgtgttct
     4021 gccctattcc ttggaagatt cgtttatttg ccgtcggtgg tcttttcgac gtgacccccg
     4081 aattggaacc gcaacgcgga gagtactttg tttccaaagt ctccctcacc cctcgacgag
     4141 aaacgctggt agagtgcggc actaagtacg ggtgcgggaa ctccttcttc gattgccgcc
     4201 tggatggtcc aacgaccttc accggagtct gaaactcgcc cggaaaactt ttccagttga
     4261 ggttgctctg ctaatgcgcc tgctgtcaga tccaacaacc acgaggcgac tacactgccg
     4321 cgacgccaga gctcagtaat atcagccagg ttaaagtcgt actggtaatg atctgggtta
     4381 cgtaaaggag tcgtttcggc gtccttggca tgatccaatt ttcctatgtt ggcatggtgc
     4441 aggacattca ggccctccgc atacgcggcc atcaagccgt actcgattcc gttatggatc
     4501 atttttacaa agtgaccggc tcctgatggt ccacagtgaa ggtatccctg ttccgctgta
     4561 ctgttgccac tcctacccgg tgtcgacgac acaacatcgc ggccaggcgc tagagtttta
     4621 aagatcggat cgaggtgagc gactatctca ttctcgccac cgatcatctg gcaataccct
     4681 cgttccaaac cccagactcc tccgctcgtt cctacatcga cgtaatgaat gcctttcgcg
     4741 cgaagttcat ttgcgcgccg aatgtcgtca atgtaataag aattgccacc gtcaatgaga
     4801 atgtcgtcct tttggattag agcgagaagt tcgttaatgg aagaatcgac aaaagctgca
     4861 ggaatcatca gccagatggg tcttggtggt tgtagttttt ctataaactc agaaagagag
     4921 ttcgatccaa ccgcaccttc ttttgcgaga tcttcgactg cagtggtgtt cctgtcatag
     4981 acgacacact gatggccacc gttagtcaaa cgctttacca tgttggctcc cattcggccg
     5041 agccctatca tgccaatctg cattcctata tctccttatt gttgttgttc gctcattcta
     5101 acccttaact ccgttcgtcg cattcagaga cggggagtcc ccattttatc gagtattgaa
     5161 ccctgtgaga gtgaggacag gctgaccgaa aatacgcatc aatctgctat tctccacatc
     5221 gcgcaatgga atcaaccgct tcagtatcag ttgagataga ccagtccaga cgttggcagg
     5281 ttcttgggct tctcgcgatt gcggagcttc tggcgatgtc actttggttc agtgtttcgg
     5341 cggtcgtccc ctcgttgcgc gctgaatgga acttgagcga agcggccgct ggttggctaa
     5401 ccgttgctgt ccagctcgga tttgttttcg ggacgctctt gagtgcgatc ttgaatctgg
     5461 ctgacatcgt ccgcgcacgt catttggtcg cgatcaccgc atttcttggc gctttcacaa
     5521 atgcgtcgtt cgctttgttt gcacacgatg cgctgatggc catcgcgttg aggttcctca
     5581 caggagtttt tcttgccggt gtgtacccgc ccggaatgaa agttatggcg agttggtttc
     5641 gacgctcgcg aggcctcgcc ctgggtgtgc tcgtgggtgc cttaactctc ggaaaggcgt
     5701 cgccctacct tatcaatgca atcggcagct caaattggcg ctacaacgtc ttagtggttt
     5761 ccacgctcgc ggcgattggt ggcctgattg tcttgttatt cgtcagcgat ggaccatacg
     5821 gtctgccacg cgcccaattc gattggcggc aggtgctgaa aatatttcgc aatcgtccgg
     5881 tgctgctggc gaactttgga tacttcggac acatgtggga gctctacgcg atgtggacgt
     5941 ggattcccgt tatgctgcgt tcgagtatgg tgttaagtcg taaggatggt gtgtgggccg
     6001 aagtcggatc gttcgtggtg ataggctccg gtgcggcggg atgtgtgctt gccggtctga
     6061 tagctgatcg ggttggacgg acgatggtca catcatgggc catgataata agtggaagtt
     6121 gttgcctgat agtcggattt ctgtatggag gggatccgat tctactcctg atcgtttcag
     6181 ctatctgggg agcgagtgtt gtggcagact cagcgcaatt ttcgtcctgc gtcacggaag
     6241 tcggcgatcc gcagtacata ggaacaactc tgacccttca gatgtgtatt gggtttctgt
     6301 taacgactgt ttcgatcgag ctgattccgc ggatggttaa tttggtgacg tggcgttatg
     6361 catttgcgat tctcgcggtc ggaccggccc tcggcgtcgt ttcaatgttg cggcttcggc
     6421 aactacctga ggccgtgaag atcgcaaacg gaagaagatg agtcaggggg gattaggggt
     6481 taggtatcat ttgagaatta tcaattgata tttgagaatt tccatttcga gcccccgtaa
     6541 gcgaattagc aattctcaaa tgtcaaatga taattctcaa atgatatcct aacctctaac
     6601 cctgaccctc tgacccctga ccctctgacc cctgacccaa tcctgaccct gacccaatcc
     6661 tgacccaatc cttgcaaatc gaaaatctat ccgttaggct ctcgcacatg ttactccctc
     6721 tccgcaagaa ttgagtttgc actgaactgg ccccgcgtgc gcgctttttg cagcagccgt
     6781 gggtcttggt tgtgctgctg atagctcctc tgtcagcagt ggcagtgcgc cttcaacttc
     6841 ctcgtt
//
LOCUS       DASSFP010000261         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1403151, whole genome shotgun sequence.
ACCESSION   DASSFP010000261 DASSFP010000000
VERSION     DASSFP010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403151"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VFH91_05745"
     CDS             complement(<1..174)
                     /locus_tag="VFH91_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6862526.1"
                     /translation="MEQSIIHIAFVVRDYDEAIAFFCHKLHFTVVEDSYQPDQDKRWV
                     VVAPPGSKGTSLLL"
     gene            complement(189..3320)
                     /locus_tag="VFH91_05750"
     CDS             complement(189..3320)
                     /locus_tag="VFH91_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862527.1"
                     /translation="MVGHLFAQAPQPSGADDYNAIFKVVKWRSIGPFRGGRSNSACGV
                     IGDPNTYYMGTTGGGLWKTDDMGISWRNVSDNYFKTGSVGAVAVAESDSNVVYVGMGE
                     HAVRGVMTHHGDGVYKSTDAGKTWKRMGLELTQHISRIVIDPKNPNIILVAAQGALYS
                     SSQQRGIYKSIDGGATWKNVLFVNDKTGAAELSMDMNNPRILYAAMWEHGRLPWKVIS
                     GGPGSGLYKSTDSGDSWERLKEGLPEEMGKMSIAVSRSNPDKVYALIESDSDKEAGGL
                     FISVDAGKKWSRITNDHRLVQRAWYYIELFVDPRNENTIYVMSAPALRSTDGGKTWET
                     LSGTHGDFHDLWINPNNPNNFIISNDGGSAVTFNGGKSWSTQSNMPTGQFYRINVDNQ
                     FPYRIYGGQQDNTSVSIASRELGGGGISTASWTFSAGGESAFLAFDPDDPKFVMGDSY
                     QGTIEVLNIKAQASTNVMAAPIQYLGMDAKDIKYRFNWNSPIIWSKHEPNTFYHGAQY
                     LLKTSDMGKTWKEASPDLTRNEKEKQGKGGGPYTNEAVGAENYGTLSYIMESPHEKGV
                     IWTGSDDGLVYLTRDGGANWKNVTPKSLEECLVNAIEVSPHDKATAYIATTRYKFNDH
                     RPGLYKTTDYGNTWEKIDDGLPANAFTRVVREDDVRRDLLFAGTELGLFVSWNAGKNW
                     SPFQLNLPITPITDLRVHKGNLIASTSGRGFWILDDLSLIRKYEKDSPPFAIYQPARA
                     YLVNGSSELDSPDDDFTGASKFRGVNPATGLVIYYQLPELKKTDEITLEIKDSAGKTV
                     RTFSSKADEKAAKWDGAPKPDPVLDKNKGLNRFVWDMRYATIPGVPGVRIEGSFAAHK
                     APPGKYILTLKMPGGQAISSAETEILANPLYPTSSSEYTEYHQTMLSMETELTSMHRM
                     INSLYEKQKQLESLLEKLPAGDKYNAVKKDGEALVKKMKAWDEDMVQRKSKAYDDVEN
                     FPNKFTANYLFLINQTESDIPRVNQPSLDRMKQLNGDWAGLKARGNELLERDLPAFNK
                     RLWELGLGAIWKE"
BASE COUNT          762 a          899 c          770 g          932 t
ORIGIN      
        1 taacaagagg ctcgtgccct tcgatcccgg aggcgctacg acgacccaac gcttatcttg
       61 atccggctga taagaatctt caaccacagt aaagtgcagt ttgtggcaaa agaaagctat
      121 ggcttcgtcg taatcacgga ctacaaaagc gatgtggatt atcgactgtt ccatactgcc
      181 cactgccact actccttcca aattgcgccg aggccgagtt cccagagccg cttgttgaag
      241 gccggaaggt cccgctccaa gagttcgttg cccctcgctt tcaatcccgc ccagtcgccg
      301 tttaattgct tcatgcgatc gagcgacggt tgattcactc gcgggatatc actctcggtc
      361 tggttgatca gaaagagata gttcgcggtg aacttgttcg ggaagttttc gacgtcgtcg
      421 taagcttttg acttgcgctg gaccatgtct tcatcccagg ctttcatctt cttcactaac
      481 gcttcgccat ccttcttcac cgcattgtat ttatcgccgg ccggtaattt ttcgagaagt
      541 gactccagct gtttctgttt ctcgtagagg ctgttaatca tccggtgcat cgacgtcaat
      601 tccgtttcca tgctcaacat cgtctgatga tattcagtgt attcggatga gctggttgga
      661 tagagtggat tcgcgagtat ctcggtttca gccgacgaaa tcgcctgacc accaggcatt
      721 ttcaaagtca gaatatattt cccgggcggt gccttgtgtg ctgcaaaact tccttcgata
      781 cgtactccgg gaactcctgg aatcgttgca taacgcatat cccagacaaa tctgttcagc
      841 cctttattct tgtctagaac tggatctggt ttgggggcac catcccactt cgcagccttt
      901 tcatctgctt ttgaagaaaa agtgcgaaca gtttttccag cagagtcttt gatctcgagc
      961 gtaatctcat cagtcttttt caattcgggc agttgatagt agataactaa cccggttgcg
     1021 ggatttactc ctctgaactt actggctcct gtaaaatcgt catccggtga gtctaattcg
     1081 ctcgagccat ttacgagata tgcccgcgct ggttggtaga tcgcaaacgg aggcgagtct
     1141 ttttcgtact ttcgaatcag actcaaatca tcgagtatcc agaatcccct tccggaagta
     1201 gatgcgatga gatttccctt gtgcactctc agatctgtaa ttggggtgat gggcaagttc
     1261 aattgaaatg gcgaccaatt ctttcccgcg ttccacgaga caaacagacc cagttcggtg
     1321 cccgcaaaca aaagatctcg gcgcacatca tcctcgcgca cgactctggt gaacgcgttg
     1381 gcaggcaagc cgtcgtcgat tttttcccag gtgttaccgt aatcggtagt cttgtacagg
     1441 cctggtctat ggtcgttgaa tttatatcga gtggtggcaa tgtaagcggt tgccttgtca
     1501 tgtggcgaga cctcgatcgc attgaccaaa cattcttcga gacttttcgg tgttacgttt
     1561 ttccaattag cgccaccgtc tcgggttaaa tagaccaacc catcgtcact gccggtccag
     1621 atcacccctt tttcatgagg cgactccatg atgtagctga gtgtgccgta attttctgca
     1681 cccacggctt cgttggtgta tggaccacca cctttgcctt gcttctcttt ttcgttgcgc
     1741 gtcaggtcgg gcgaggcttc tttccatgtt ttgcccatat cactcgtttt cagaagatac
     1801 tgagcaccat gatagaaagt attcggctcg tgtttgctcc agataatcgg tgaattccag
     1861 ttaaatcgat acttgatgtc ttttgcatcc atccccaaat actgaatagg tgcagccatc
     1921 acgttggtgc tggcctgggc cttaatgttg agtacttcga tcgtaccctg ataactgtcg
     1981 cccatgacaa attttggatc gtccggatca aacgccagaa acgcactctc gccaccggca
     2041 gaaaatgtcc agcttgcagt gctaatacct ccgcccccta actctcggct cgcaatcgac
     2101 accgatgtat tgtcttgctg tcctccatag attcgataag gaaactgatt atcgacgtta
     2161 atgcgataga actgtccggt cggcatgttg ctttgggtgc tccaactctt tcctccgtta
     2221 aaagttactg ccgagccacc gtcgttggag attatgaagt tattagggtt attgggatta
     2281 atccaaagat catgaaaatc gccgtgagtg ccagacagtg tttcccaggt cttccctcca
     2341 tcagtcgaac gcagggcggg cgcgctcatc acataaatcg tattctcatt cctgggatca
     2401 acgaacaact cgatgtaata ccacgctctc tgcactagcc gatgatcgtt ggtaatacgg
     2461 ctccacttct tgccggcatc aactgagata aagagaccgc ccgcctcttt atcagaatcg
     2521 ctttcgatca gcgcgtaaac tttgtccgga tttgaacgac ttacggcgat cgacattttg
     2581 cccatctctt ccggcaagcc ttccttcagt cgttcccagc tgtcgccact atcggtggat
     2641 ttataaagac cgctgcctgg gccgccacta atcactttcc atggaaggcg cccgtgctcc
     2701 cacattgcag cgtaaagtat tcgaggattg ttcatgtcca tcgacaattc cgccgcaccg
     2761 gttttatcgt tcacaaacag gacatttttc caggtcgccc cgccatcaat ggatttataa
     2821 ataccccgtt gttgtgaaga gctgtataac gcgccttgag ctgcaaccag gatgatgttt
     2881 ggattcttcg gatcaataac gattcgagaa atatgctggg ttaattccaa tcccatcctc
     2941 ttccaggttt tgccggcgtc cgtcgacttg tacacaccat ctccgtggtg agtcatgaca
     3001 ccgcgcactg catgctcacc catacccaca taaacgacgt tcgagtcgct ttcggcgaca
     3061 gcgacagcac cgactgaacc ggttttgaaa tagttatccg agacattacg ccagctgatc
     3121 cccatatcgt cggttttcca aagaccgccg ccggttgttc ccatgtagta agtgtttgga
     3181 tcgccaatca ctccacatgc cgagtttgag cggccgccac gaaatggtcc aatagatcgc
     3241 catttaacta ctttgaaaat tgcgttgtag tcatccgcac ctgaaggctg tggggcttgc
     3301 gcgaaaagat ggcccaccat caagcagaac acgacaattg aaaacaggag ttttgacatt
     3361 cag
//
LOCUS       DASSFP010000262         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1403531, whole genome shotgun sequence.
ACCESSION   DASSFP010000262 DASSFP010000000
VERSION     DASSFP010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403531"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFH91_05755"
     CDS             <1..470
                     /locus_tag="VFH91_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862528.1"
                     /translation="KDLDDDSNERDPFTDRSFNRFDLSLQYSPSDRDIRHKFNLYAFF
                     ELPAGFELNPRFQARSAQPITPSPRTLNGVDRGRNSVRKDNQFASFDWRLQRPFNFGD
                     RYALIPIVEMFNTFNADNNINPLVTPALFNFDGFLRSGVGDPRQLQLAVRFTF"
     gene            863..3574
                     /locus_tag="VFH91_05760"
     CDS             863..3574
                     /locus_tag="VFH91_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6862529.1"
                     /translation="MLDEKQEQTHFLKSLLEANVDGIIAFDREFRYTAWNRAMERLSG
                     VKREEVLGKNAFEVFPCLKEIGEQKYFQAALDGNSIVAENRPYTVAQTGRSGVFDGYY
                     SPRYGEDGDVVGGVAIIRDITEQKLAERSAQEAHKRLAFHVENTPLAVIEWDQEFKVL
                     RWSPAAERLFGWTSQEVLGKRFSDWRFVVPEDVEAVHAVEQRQATEQEVHGVSRNRNF
                     SKVGNVVHCEWYNSALYDETGKLISVLSLVLDITMATRIEDALRKSEAQYRLLFESNP
                     HAMWVYDLKTLRFLAVNSAAVRHYGYTRPEFLAMTVEDIHPPEDCDELSEHLVVDSLR
                     DQAYQARHRRKDGLVIDVEITSHPLDFGGRRAALVLANDITQRKCAEQALLESEDRYR
                     DLVENSHELMCTHDLDGRIISVNPWAAKILGYKQESLVGMNIRDGLLPEFRNQFDDYI
                     DRVKTEGSARGVMRVRTASGETRLWEYYNTLRTQGVETPIVRGMAHDATERRKALDRE
                     KEARLEAEAANRVKDEFLSTLSHELRTPLTAIMGWSNLLLDGEVAPEKQRQAIETIAR
                     NANSQCQLIDDLLEVSRIITGKLRLDFAPCALQPIIQAATESIRPTAEAKGVRLECSL
                     PQDIDVVHGDCERLQQVVWNLLSNAVKFTPTGGSVHVELKQVNSHAAITVRDSGTGIK
                     RDFLPYVFDRFRQADGSTTRAFGGLGLGLAIVRHLVELHGGNASAESEGENKGSTFVV
                     KLPLVQSMDHALHPLHQPASRLAIASRSAPATGLTGIRVLMVDDEFDARELVVTMLEK
                     DGAQVRAVGSAKEAIEEIGTWKPDVLIADIGMPDEDGYGLIRRVRALSKEQGGQTPAL
                     ALTAYARTEDRVRALSAGYQVHLSKPVDRSELAAVISRLRVGVGRVQ"
     gene            3633..4406
                     /locus_tag="VFH91_05765"
     CDS             3633..4406
                     /locus_tag="VFH91_05765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6862530.1"
                     /translation="MKLVACYLLSIVLLPFYQQASPRHIVDLTYPFDSTTVYWPTAEG
                     FKLETDFEGMTDKGYYYSAYRYSGAEHGGTHLDAPVHFAKGKNSIDQIPLEQLMGPAL
                     VIDVSRQCATNADYLVTVTDFENWEKQNGKIASGSIILLKTGYGKLYPDRKKYLGTDE
                     RGAEAVAKLHFPGLDPAAAKWLAQNRSIKAIGLDTASIDRGQSTLFESHRTLFENNIP
                     AFENVANLDQLPAKGFTVIALPMKIKGGSGGPLRIIATW"
BASE COUNT         1129 a         1179 c         1125 g         1022 t
ORIGIN      
        1 cgaaggatct cgacgacgac tcgaacgagc gcgatccatt cactgatcgc tcgttcaatc
       61 gctttgacct ctcgcttcaa tactctccgt cagatcgaga catcaggcac aagttcaacc
      121 tctatgcatt ctttgaacta cccgcaggct ttgaactgaa ccccagattt caggcacgtt
      181 cagctcaacc gatcacacca agcccacgta ctttgaacgg cgtggatcgc ggccgtaact
      241 cagtgcgaaa ggataaccag tttgcttcgt ttgactggcg tctccagcgt ccgttcaatt
      301 ttggagaccg ctatgcgctg atcccaatag tggagatgtt caatacattc aacgccgaca
      361 acaacatcaa cccactggtt actcccgcac tgttcaactt tgacggcttc ctgcgttcgg
      421 gtgtcggcga cccgcgacaa ctgcagcttg cagtcaggtt cacgttctga gtggctaaca
      481 attgaaccac gcatctggcc tggggtctcc ggatcccagg tctttttttg atcactaatg
      541 tagttgaagg tttgcagctc accaaaaaat tatccgcaga ttacgccgac tacacagatt
      601 ttcaactcaa atggtggtgt ctaactcgtc agacgttttg aaatcggcgt aatcagtgca
      661 atctgtcgat gatcctctct tttgctctcc ccatcggcaa cgacaacagc aattctgcaa
      721 tttccagatc actcttgaca tttcttcagc catgattaga gtcatagcca ctcgtccatc
      781 gcgaaacccg aattttgagc gttgacctct taattttcat cagcaactta cttaccatcg
      841 aaccccagcg agaacgatat aaatgctcga cgaaaaacaa gaacaaactc atttcctgaa
      901 atccctactt gaggcaaacg tcgacggcat catcgccttc gatcgcgagt ttcgctacac
      961 ggcctggaac cgggcaatgg aacgattgtc aggtgtcaaa cgggaagaag tactcggcaa
     1021 gaacgcgttc gaagtttttc cctgtctcaa agagattggc gaacagaaat atttccaggc
     1081 tgctttagac ggcaatagta tcgtcgccga aaatagaccg tacaccgtag cgcagaccgg
     1141 tcgatcagga gtcttcgacg gttattactc ccctcgttat ggtgaggacg gagatgtagt
     1201 cggcggggtt gcgattatcc gcgacatcac cgaacaaaaa ctcgccgagc gatcagctca
     1261 ggaagctcac aagcgactcg cttttcacgt tgagaacact ccgctcgcag ttattgagtg
     1321 ggaccaagag ttcaaagtgc ttcgctggtc accagccgct gagcgtctgt tcggctggac
     1381 ttcacaggaa gtactgggca agcgattcag cgactggcgc ttcgttgttc cggaagacgt
     1441 agaagccgtg cacgctgtcg agcaacgcca ggcaacagaa caggaagtac acggcgtctc
     1501 tcgaaatcgt aatttcagca aagtcggaaa tgtcgttcat tgcgaatggt acaactcggc
     1561 actctatgac gagacgggaa aattgatctc agtcttatct ctcgtgctgg atatcacgat
     1621 ggccacgcgc attgaagacg ctcttcgaaa atcagaagca caatatcgtt tactgttcga
     1681 gtcgaatcca cacgcgatgt gggtttacga tctgaagaca cttcgcttcc tcgctgtcaa
     1741 ctccgccgcg gtgcgccact acggctatac ccgacccgag tttctggcaa tgacagtgga
     1801 ggacattcat cctccggaag actgtgatga gctgagcgaa catctggtcg tggactcgct
     1861 acgtgatcaa gcgtatcaag cccgccaccg caggaaagat ggactcgtaa tcgatgttga
     1921 aatcacttca catccgctcg attttggtgg tcgacgggca gcgcttgttc tggcaaacga
     1981 cataacgcaa agaaagtgcg ccgaacaagc tttacttgaa agtgaggatc gctaccgcga
     2041 tctggttgag aatagccatg aactgatgtg cacacacgat ctcgatggtc gcatcatctc
     2101 agtgaatcca tgggcggcaa agatcctcgg ttataaacaa gaatcgctcg tgggaatgaa
     2161 tatacgcgac ggactattac cggaatttcg caatcaattc gacgactaca ttgaccgggt
     2221 taagaccgag ggctcagcgc gaggtgtgat gcgtgttcgt acggcatccg gcgaaacgcg
     2281 actctgggaa tactacaaca cgcttcgcac gcaaggcgtc gaaacaccca ttgttagagg
     2341 catggcccat gacgctaccg aacgccgaaa ggccctggac cgcgagaaag aggcacgact
     2401 tgaagctgaa gctgccaacc gcgtaaagga tgagttctta tcaacccttt cacacgaact
     2461 gcgcacacct ctcacagcca tcatgggttg gtccaacctt ctgcttgatg gtgaagtagc
     2521 tcctgaaaaa caacgccagg caattgagac gattgcgcgc aacgcaaact cgcaatgcca
     2581 gctcatagat gatctgctcg aggtttcccg gattatcacc ggtaaacttc gtttggattt
     2641 cgcaccctgt gcgcttcagc caatcattca agctgctaca gaaagtatca gacctactgc
     2701 cgaggctaag ggtgtacgac tcgagtgttc gctgccacaa gacatagacg tcgttcacgg
     2761 cgattgtgaa cgactccagc aagttgtctg gaatttactt tccaacgccg tcaaattcac
     2821 gcctacagga ggttctgtcc atgtcgaact caaacaggtc aactcgcatg cagctataac
     2881 tgtgcgagac tccgggaccg gcattaaacg ggacttcctg ccatatgtgt tcgatcgctt
     2941 tcgccaggca gacggctcga ctacaagagc gttcggcgga cttggtcttg gtctggcaat
     3001 tgtgcgacat ctggtcgagc tacatggcgg aaacgcctcc gcggaaagtg aaggcgagaa
     3061 taaaggttca acctttgtag tcaagcttcc tctcgttcag tcgatggacc atgcgctgca
     3121 cccgctacac caacccgcat caagattagc gattgcatca cgcagcgctc cggcaacggg
     3181 tttaaccggc attagagtct tgatggtaga tgatgagttt gatgctcggg agctggtggt
     3241 gacgatgctg gagaaggacg gtgctcaggt aagagccgtg ggttcggcta aagaggcaat
     3301 cgaagagatt ggcacgtgga agcctgatgt gctgatcgcc gatattggaa tgccggatga
     3361 agatggctac ggattgatcc gaagagtgcg ggctttgtcg aaggaacaag gaggtcagac
     3421 acccgcgctg gccctgactg cctatgctag aactgaggat cgggtgcgag cattgtcagc
     3481 cggctatcag gtccatcttt ccaagcctgt ggaccgatct gagctggctg cagtaatttc
     3541 tcgtctgcga gttggagtcg gtagggttca ataactccag gcccggatta ccaaagcctc
     3601 gccgggccgg ctcattccca aaggacaaca acatgaaact tgtcgcgtgc tatcttctct
     3661 caatcgttct attgccgttc taccagcaag cctcgcccag gcatattgtc gatcttactt
     3721 atcctttcga ctcaacaacg gtctattggc ccacagcgga gggtttcaaa ctcgagactg
     3781 atttcgaagg aatgaccgac aaaggctatt actattcagc ctatcgttat tctggcgctg
     3841 agcacggcgg aactcacctg gacgcgccgg tgcatttcgc caaagggaaa aacagtatcg
     3901 accaaatccc cctcgagcaa cttatgggcc cggcactcgt aattgacgtc agccggcaat
     3961 gcgccaccaa cgcagactat ctggtaaccg tgaccgattt cgaaaactgg gaaaagcaga
     4021 acggaaagat cgcgagtggt tcgatcattt tgctcaaaac tggctacggc aaactttatc
     4081 cggaccgaaa aaaatacctg ggtaccgacg agcgcggcgc tgaggcagta gcgaagctcc
     4141 atttccccgg tctcgacccg gcggcagcta aatggttagc tcaaaataga tcgattaagg
     4201 caattggtct tgacaccgcc agcattgatc ggggccaatc aactctgttt gagagtcacc
     4261 gcacgctttt cgaaaacaac attcctgcat ttgagaatgt cgctaacctt gaccagttgc
     4321 cggctaaggg ttttacagtc atagcgctgc cgatgaagat caaaggtggt agcgggggcc
     4381 cactcaggat aattgctact tggtagcgct ttgcactttg tgcattgtgc tcacaaagta
     4441 cgaagtacaa agtac
//
LOCUS       DASSFP010000263         5896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_966773, whole genome shotgun sequence.
ACCESSION   DASSFP010000263 DASSFP010000000
VERSION     DASSFP010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966773"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFH91_05770"
     CDS             <1..537
                     /locus_tag="VFH91_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862531.1"
                     /translation="KFYVRHIFLQHDSAGHGKITFERLNEDVAVEEPLEISSTALSRI
                     RSSWDALKFLDSQKDYQSSQQFPHLGTMKIGMQLGEKKRIAEFNWTHDQNAADLIAEY
                     RKLADQAILVFDISVARENQPLNAPKLMETMESLLKRNGLSDPKQLLPLLQDISTDEH
                     LPLIARNHALRLIKQIKK"
     gene            complement(527..1624)
                     /locus_tag="VFH91_05775"
     CDS             complement(527..1624)
                     /locus_tag="VFH91_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6862532.1"
                     /translation="MVLDHAVYDHNISTEHLNYVKRLFAVVRAAVEQKPFRPAALLRQ
                     ADLQTLAAYVWPRRRRLKDSYALDEERLFAVAPDVQVLSLCRWHEDRHTKPTVILWHG
                     IEGSASAVYMLSFANKAFIAGFNVVRVNIRNCGGTEHLTPTLYHGGLSEDLRSIIEEL
                     IQRDGCKKIFALGFSLGGNMVLKMAGEYGDRAPAELIATCAISPSVDLAASAQLINRK
                     RNWLYHQSFVVKLKRKIHLKHRLYPDLYNLTYVKGIRTLQDFDEAYTSKANGFSDAQD
                     YYTKASAARVAKNIRVPTLILHSQDDPFIPFAPLKAKEFSDNPYILVVGPATGGHVAF
                     VSQKTSADDRFWAENRALEFFRLALEQLATS"
     gene            complement(1636..2970)
                     /locus_tag="VFH91_05780"
     CDS             complement(1636..2970)
                     /locus_tag="VFH91_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET6862533.1"
                     /translation="MKPLRNQRLFHCLLLVLILIVSSVVVTGQPQESSRITKIEFDGL
                     KSLSAEEVLVISTLKVGETFDVAALDSAAQKLADSGLFKNVAYRTRNNKGLVTIAFQL
                     EEISRPISRVIFDNFIWFTDTELIAAVKRDVPSFSGAAPENGDMVDRITRALQTFLHE
                     NKIEASVSYLASQDSPESTVQEHVFSVTGLPMPICTLHFPGATNISEQRLIESSKTLV
                     GGEYSSKFVSLFTAKNLSPLYREAGQLRAAFSPPLPKPEATATCKSGVEVSLPVDEGL
                     IYKLGNVKWSGNTSLNASKLDSLLTIKSGEIANGVRLDKDLAAAQKAYGRQGFLLARI
                     KQHPEFKDETQTVNYVFDIAEGPQFRMGKLILQGFGDNISKQITDQWRLKSGDVFDNG
                     YEEEFSRNEFGELLKPIHMERRSQGKPAPSVTSSMKLNRETLTVDVTLELTN"
     gene            3090..3632
                     /locus_tag="VFH91_05785"
     CDS             3090..3632
                     /locus_tag="VFH91_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862534.1"
                     /translation="MKSKQLTKREVILQEWRRLKTDLVGSNELKGINEALLKVFGPGG
                     AESPASLARLLADHGARLKHPEVLETDTRWREQRLDLLFAHGEIDFNKIASSIESISK
                     MDDLYRQFLAEGDDAGLEQVREFAKELKFELRRSTSTALTAEVGEWLTIWLQTPQMFA
                     EWLDLRIKSPGFQAKFGSEL"
     gene            3702..4319
                     /locus_tag="VFH91_05790"
     CDS             3702..4319
                     /locus_tag="VFH91_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6862535.1"
                     /translation="MRPHSELNDTELLRSMMDGDEEALGALYQRRHAGVFRFAWQMSG
                     SHTVAEDVTQEVFLFLIRDRHVFDPDKGSVNAFLMGVARNYVLRRLRGERTMISLGDD
                     SEDDFSGELADPDLSPLEDLTRTETIENVRKAVLSLPEKYREVVVLCELQELNYKETA
                     EILECAIGTVRSRLHRARAILLEKLRPARAETEEGTALKSAGCFA"
     gene            4316..5098
                     /locus_tag="VFH91_05795"
     CDS             4316..5098
                     /locus_tag="VFH91_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862536.1"
                     /translation="MNCQKFETIVSELARDQMMEADIRAEALAHTFKCSECSDRMRDQ
                     KLLSSGLNALASDMNRLEAPPQLELKLREAFRQSRQVAPFPLSKRSNSRYWLSAVAAL
                     LLVVIGLAVVRARVVGIQKQLAEIPAQSIPAYEVKTPDVTSTRENVIAGNNVKIESPR
                     RRQQQSSSLYAKNTRRHTARNDAVVAANHVTREVATDFMPLGYMNAANFQDGGQLVRV
                     ELPRSALAKFGIPVNIERYNEKVKADVLFGADGLAHAIRFVQ"
     gene            5145..>5896
                     /locus_tag="VFH91_05800"
     CDS             5145..>5896
                     /locus_tag="VFH91_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862537.1"
                     /translation="MKRILAILSTVFGTIACAVVVSLAQQPATQTNIVIKQGRVLQGP
                     EGGPPPPRGDFIFVATDFNFEGKVVKGAPYSAEAVTETIQTLSDGNRIVNKITSQVYR
                     DGEGRTRREQTLKGFGMLGNVGEPLQTIFINDPVAGVTYALDSRTHTAHKSAPFKFEW
                     NSKPPAPNAPPLEEGQRFEFKVVAPGSPAGGPQVMTAPLPPGALPAGENVTLSTAGAV
                     GGATYVFRRKGTDANEVKEDLGEQLIEGVKAQG"
BASE COUNT         1486 a         1349 c         1566 g         1495 t
ORIGIN      
        1 aagttttacg tcagacacat ttttctccaa catgactcgg cgggtcatgg aaagattaca
       61 ttcgaacggt tgaatgaaga tgtcgcagtg gaagagccgt tggagatctc ttcaactgcg
      121 ctgtctcgca ttcgttcaag ctgggatgcc ctgaagtttc ttgactcgca aaaggactac
      181 cagtcgagtc agcagtttcc tcaccttgga acaatgaaga ttggcatgca gcttggcgaa
      241 aagaagagaa tcgctgagtt caattggact cacgatcaaa atgcggctga tctaattgcc
      301 gaatatcgaa agctggcgga ccaggccatt ctggtttttg atatttcagt agcgcgtgag
      361 aatcagccgc taaatgctcc gaagctgatg gagacgatgg aatcgttgct gaaacgaaat
      421 ggactttccg atccaaagca gctcctgcct ctgctgcaag atatcagtac cgatgaacat
      481 cttcctctta tcgctcgtaa ccacgccttg cgcttaatca aacaaattaa gaagtagcca
      541 actgctctaa ggctagtcgg aagaattcga gagcacgatt ttcggcccag aacctatcgt
      601 cggcgctcgt tttctgagaa acaaacgcaa cgtgacctcc tgttgctggt ccaactacca
      661 ggatgtacgg attgtcgctg aattctttcg cctttaaggg agcgaatgga atgaaggggt
      721 cgtcctgcga gtgaagaata agggtgggta cacgtatgtt ttttgcgacg cgtgctgcac
      781 ttgccttcgt gtaatagtct tgagcgtccg agaatccgtt tgcttttgag gtgtaagctt
      841 catcaaagtc ctgcagagtc ctgatccctt taacgtaggt cagattgtag agatcgggat
      901 aaagacgatg ttttaagtga atctttctct tgagctttac aacgaaactt tgatgataca
      961 gccagttccg tttcctgttg attaactgtg cgctggctgc cagatctacc gatggagaaa
     1021 tcgcgcatgt tgcaattaat tctgcaggag cgcgatcgcc atattctccg gccatcttaa
     1081 ggaccatgtt gccacccagg gagaacccaa gagcaaagat ctttttgcac ccgtctcgct
     1141 gaattagttc ttcaataata gaacgcaaat cctcgcttag cccgccatga tagagcgtag
     1201 gtgtcagatg ctcggtgcca ccacagttcc gaatattcac tcgcacgacg ttgaatcctg
     1261 caataaacgc cttgttagca aatgagagca tgtaaacagc ggatgcggag ccttcgatac
     1321 cgtgccacaa aattacagtt ggcttcgtat gacggtcttc gtgccaccga cagagtgata
     1381 acacttgtac gtcgggggca acggcgaaca atcgttcctc gtcgagagcg taagaatctt
     1441 ttaaacgacg tcgacgtggc caaacatagg cggcgagggt ttggagatcg gcttgtctca
     1501 gtagagctgc tggtctgaat ggcttctgct ccacggcggc tcttaccacc gcgaacagtc
     1561 ttttgacgta gttcagatgt tcggttgaaa tgttatggtc gtagactgcg tgatcaagaa
     1621 ccatctcatg aagaactagt tagtcagttc aagtgtaacg tcgacagtta gggtttcccg
     1681 gttcaatttc atgctcgatg tcactgaggg cgctggcttg ccctgcgatc tgcgttccat
     1741 gtgtatcggt ttcagtaatt caccgaactc gttccgcgag aattcttctt cataaccatt
     1801 gtcaaaaaca tcacctgatt tcagtcgcca ctgatctgta atctgctttg agatgttatc
     1861 tccgaatccc tgcaggatta acttgcccat tcgaaattgt ggcccttcgg caatgtcaaa
     1921 aacataattg acggtttgtg tttcgtcttt gaattcgggg tgctgcttaa ttcttgccaa
     1981 caaaaagcct tgccggccat aggctttttg cgcggcagcc agatccttat cgagtctgac
     2041 gccgttggca atttctcctg attttatggt caggagtgaa tcgagcttcg aggcatttaa
     2101 tgatgtattg ccggaccatt tgacgttgcc cagcttataa ataagtccct catcgactgg
     2161 taagctgact tcgaccccac tcttgcaagt tgcagttgct tcaggcttag gaagaggagg
     2221 cgaaaaagct gccctgagtt gaccggcttc gcgataaaga ggagagagat tttttgcagt
     2281 gaacaaactg acgaatttac ttgaatactc gccaccaaca agagttttcg agctttcaat
     2341 taacctctgt tcagaaatgt tggttgcgcc cggaaaatgc aacgtgcaaa ttggcattgg
     2401 tagaccagtt acactaaaga catgttcctg aaccgtactc tctggtgagt cctgcgaggc
     2461 gaggtatgaa acgctagctt caatcttgtt ctcgtgaaga aacgtttgaa gcgctcttgt
     2521 tatgcggtcc accatgtcgc cgttctctgg agctgcgccg gagaatgaag gtacgtcgcg
     2581 tttgacggcg gcgatcaact ctgtgtcggt gaaccaaatg aaattgtcga atataactcg
     2641 agagataggt ctgctgatct cttcgagttg aaaggcaata gttacgagtc ctttattatt
     2701 ccgagtgcga taagctacgt tcttgaacag accgctatcg gcgagctttt gcgcggctga
     2761 atccagtgcg gcaacatcaa aggtctctcc taccttgagg gtagagatga cgagcacctc
     2821 ctcggccgag aggcttttga gaccgtcaaa ttcgatcttg gttatcctgg aagattcctg
     2881 gggttgacca gtgacgacaa cgctgctgac aatgagaatt aagactaata aaaggcagtg
     2941 gaaaaggcgt tggtttctga gaggtttcat gggaacaaga tcgtattacc agcggagtgt
     3001 atcgcattca atgtaggggg gagaagcaac cagagcttag agtcgagtct taacttggct
     3061 tactctgtgg aacctttgtg tccttgaaca tgaagagcaa gcaattgacc aagcgagaag
     3121 tcatccttca agagtggcga aggctgaaga ctgacctggt cggtagtaac gaactcaaag
     3181 gaattaatga agcgcttctc aaagtatttg ggccgggtgg agccgaaagt cctgcttctc
     3241 tagctcggct gttggcagat catggcgcgc ggctcaaaca cccggaggtg cttgaaacag
     3301 acacgcggtg gcgcgagcag cgactcgatc tattgttcgc ccatggagaa atcgatttca
     3361 acaagatcgc aagctcgata gagtcgattt cgaaaatgga cgacttatac cgtcagtttc
     3421 tcgccgaagg tgatgatgct ggattggaac aggtccggga atttgcaaag gaactcaagt
     3481 tcgagttgag acggtccact tcaaccgcgc taacggcgga ggttggcgag tggctgacaa
     3541 tctggctcca aacaccccaa atgttcgccg aatggctgga tttacggata aaatccccag
     3601 gttttcaggc gaagttcggt tctgagctct aaaacatcac ttattggaac tttcgacgct
     3661 atcctcgcga ataaggtatg tcaggcgaac tcttaacgcg aatgagacca cattctgaac
     3721 taaatgacac cgaattgctc cgttccatga tggatgggga cgaggaagct ctgggagcgc
     3781 tttaccagcg ccggcacgcg ggtgtcttcc gctttgcctg gcaaatgagt ggctctcata
     3841 cggtcgcgga agatgtgacg caggaggtct ttcttttctt gattcgtgac cggcacgttt
     3901 ttgatccaga taaaggatct gtaaacgcgt ttttgatggg tgtcgcccgc aattatgttc
     3961 tcaggcgttt acgtggcgaa cggacgatga tttctttggg agacgattcg gaggatgatt
     4021 tttccggaga actggctgat cccgacctga gtccgcttga agatcttacg cggactgaaa
     4081 ccatcgagaa tgttcggaaa gctgttttga gtttgcctga gaaatatcga gaggtcgtcg
     4141 ttctttgtga gttgcaggag cttaattaca aagaaacggc tgagattctt gagtgcgcga
     4201 ttggtaccgt cagatcgcgg ttgcatcggg cacgcgcgat attgctggaa aagttgcgac
     4261 cggcaagggc ggagactgaa gaaggtactg ctttgaagtc agcggggtgt ttcgcatgaa
     4321 ctgtcaaaaa ttcgaaacta ttgtaagtga gctcgctcgc gaccagatga tggaagccga
     4381 cattcgtgcc gaggctttgg cccacacgtt taagtgttca gaatgctctg atcggatgcg
     4441 tgaccaaaaa cttttgagtt ccggacttaa cgcattagcg tcggacatga accgattgga
     4501 agcgccgcca cagttggagt tgaagttacg agaagcgttt cgtcaatcga gacaggttgc
     4561 cccgttcccg cttagcaagc gcagtaatag tcgatactgg ttgagcgcgg tagctgcact
     4621 cttattagtt gtgattggtc tggctgtcgt tcgggcgagg gtagttggta ttcaaaagca
     4681 gctggctgaa atccctgcgc agtctattcc agcgtatgag gttaaaaccc ctgacgtaac
     4741 ttcaactcgt gaaaatgtaa tcgctggaaa taacgtgaag attgaatcgc cacgacggcg
     4801 acagcaacaa tcttcttcct tgtatgcgaa aaatactcga aggcatacag cgcgaaatga
     4861 cgcggtagtt gctgcaaatc atgtcacgcg tgaagttgca actgatttta tgccgctcgg
     4921 atacatgaac gctgcgaact tccaggatgg aggacagtta gttcgagttg agctgccacg
     4981 atctgcgttg gccaagtttg gcatacctgt gaacatcgag cgttacaacg aaaaggttaa
     5041 agctgacgtg ttgtttggcg ctgatggtct ggcccatgca atcagatttg ttcagtgagt
     5101 gattcgaacg tccggtgagt gccggttgaa aggaaaagag agtaatgaaa agaattttag
     5161 caattctgtc gactgtgttc ggaaccatag cttgcgctgt ggtcgtatct ctcgcgcaac
     5221 agcctgcgac gcagaccaac atagtgatca agcaaggccg ggtccttcaa ggacctgagg
     5281 ggggaccgcc accgccacga ggtgacttta tcttcgtagc caccgacttt aacttcgagg
     5341 gtaaagttgt taagggcgct ccttactcag ccgaagctgt gaccgagaca attcaaacac
     5401 tcagcgatgg caatcgaata gtcaataaga ttacgagtca ggtttatcgc gatggcgaag
     5461 gccgtacgcg acgtgaacag actctaaaag gattcgggat gcttggaaac gtgggtgagc
     5521 cactgcaaac catcttcatc aacgatccag ttgccggtgt gacctatgca ctcgattcgc
     5581 gcactcatac ggctcacaag agtgccccat tcaaatttga atggaactca aagcctcctg
     5641 cgccaaatgc gccaccactg gaagaaggac aacggttcga attcaaggtt gtcgctcctg
     5701 gatctccggc cggtggccct caagtcatga ctgctccgct gccccctggt gcgttgcccg
     5761 ctggagagaa cgtaactcta agtaccgcag gtgctgttgg cggtgcgact tacgtattca
     5821 gacggaaagg cactgacgcc aatgaagtca aagaagatct gggcgaacag ttgattgaag
     5881 gagtcaaggc acaggg
//
LOCUS       DASSFP010000264         5439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1557624, whole genome shotgun sequence.
ACCESSION   DASSFP010000264 DASSFP010000000
VERSION     DASSFP010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557624"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..949
                     /locus_tag="VFH91_05805"
     CDS             191..949
                     /locus_tag="VFH91_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6862538.1"
                     /translation="MRHISALKRERIDKLLVDHGLAPSRTKAQAMVMAGVVLVDDQRV
                     EKPSEQYSLQANIRIKGAEDPTSRYVGRGGLKLEGALRSFNLDVSGMNCLDVGASTGG
                     FTDCLLQNGAKKVWAIDVGHNQIDWRLRTDPRVEVREGVNARYLQPADLMVSFDLAVI
                     DVSFISVKLVLPAIVPLLKDDGRVITLIKPQFEVGRGQVGSGGIVRDEEQHRRVVAEV
                     NELAESMGLRLVNVIDSPILGAEGNKEFLALYSR"
     gene            1272..2150
                     /locus_tag="VFH91_05810"
     CDS             1272..2150
                     /locus_tag="VFH91_05810"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET6862539.1"
                     /translation="MSSIKRIGIFIKPHQPEVLRTICELTQWLTERGISLVGGPEIDR
                     ERIEHETGCPVEQVDADKLAASVDLFLVLGGDGTMIATARLIGDHEVPVLGVNYGGLG
                     YLAEFRIEELYLALESILSDNYRLDKRVMLAVEMYRGKEPAIHNRVLNDVVINKSALA
                     RIVEIEAYLNQQFVNSFRADGLIISTPTGSTAYNLSAGGPVIFPSMNAVVITPICPFT
                     LSNRPIVVPDDALIELRLKTEQEDVSLTLDGQVGLPMKTEDLVVIRKSKTTFNLIQPS
                     NRNYFDVLRDKLRWGR"
     gene            2180..3631
                     /locus_tag="VFH91_05815"
     CDS             2180..3631
                     /locus_tag="VFH91_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HET6862540.1"
                     /translation="MPEDNNKRFSGQLDPAGFEHKTTTDLDEETPDKPKGLALHHRIL
                     IGLAVGVIAGITVNHFFGGENPKVVWVIDNFTQPVGQLFLRLLLMIVVPLVFSSLVVG
                     VAGIGDIRKLGRVGLKSFAYCLVISGISAIIGITLANTIKPGKRIDPSTSAALQQRYA
                     TDASRQVDDAKKTSAQAQKPLMAVVETIVPKNPFYAVAGKEDPNMLHVMFFALVIGIA
                     ITLIPVSVTGPLLRVLEGLYEVTAKIIEMIMKFAPFAVACLLFNNTARFGLDLLQALA
                     WFVVTVLLGLSLHMFGVYSLSVYFLSRISPFEFFKRIRTVILTAFSTSSSNATLPTAL
                     RVSEQNLGVPQEINSFVLTVGATANQNGTALYEGVTVLFLAQLAGVDLSLGQQLMVVY
                     LAILGGVGTAGVPSGSIPFIIGVLVTIGVNPALIAIILGVDRILDMCRTTLNVAGDIT
                     AATFVARSEGYELLRPHEPAIVHAGVAMDRALD"
     gene            3704..4474
                     /locus_tag="VFH91_05820"
     CDS             3704..4474
                     /locus_tag="VFH91_05820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6862541.1"
                     /translation="MVLPLYDDNSDRTITPVVNYAIIAVNIFVFVFLQQLGANQQFTY
                     AFSTVPLEILTGHDVVTQSRVLLDPVSGQRVLVPGLGETPFSVYITLITSMFMHGGIA
                     HIAGNMLFLWIFGDNIEDRLGHVRYLVFYLLCGVLASLAHVFTTAAFAGGNENSLIVP
                     SLGASGAISGVLGGYILLHPTRRVTVILFRFLTDVPAYVAIGIWFAFQLISGLGMLGG
                     GSQQGGVAYAAHVGGFVAGLVLIKLFDMGHRPRQLPGY"
     gene            4578..4991
                     /gene="queF"
                     /locus_tag="VFH91_05825"
     CDS             4578..4991
                     /gene="queF"
                     /locus_tag="VFH91_05825"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET6862542.1"
                     /translation="MTTLVPPPNQQELDIQSTPRQEMRLFPLDTFPYEFPGKEISINF
                     EITEFTAICPFSDFPDFATIRMEYTPNERCIELKSLKLYINSFRDIKIFHEHVINVIL
                     EDFVAACDPLRVYIEGDFHVRGNIKTIVKANYERN"
     gene            5007..5216
                     /locus_tag="VFH91_05830"
     CDS             5007..5216
                     /locus_tag="VFH91_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862543.1"
                     /translation="MDHTFLYSILAGIVFVFIIVAARLAFRWLVRIALVGIILLVVVG
                     SLAWWWFRGPARPQTETRPTTSRRR"
     gene            5230..>5439
                     /locus_tag="VFH91_05835"
     CDS             5230..>5439
                     /locus_tag="VFH91_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862544.1"
                     /translation="MADTDTDAADQYDASIENIKLGESTPGLWQRYRRLPRNVFAISL
                     VSLLNDASSEIIYPLLPIFLVSTLGA"
BASE COUNT         1261 a         1353 c         1361 g         1464 t
ORIGIN      
        1 tggtctgatg cacctaacat tctggtctgg ctttgtcacg actgcgccgc ggaccttcgc
       61 ctgactgaaa ctccgcgagc ctttacccaa accgcccgca agcaggccta gcgggtccta
      121 attgccagca atttaattaa aattaagcac ttcactctgc ttgtgatgta atgtgcaacg
      181 catctcaaag atgaggcaca tttctgcgtt gaagcgtgaa cgaatcgata agctgctcgt
      241 cgatcatggc ctggctccct ctcgcaccaa agcgcaagcg atggtaatgg cgggcgtcgt
      301 gctggttgat gaccaaagag ttgagaagcc ttccgaacag tattctctgc aagcaaacat
      361 cagaattaaa ggagccgaag acccaacctc tcgatacgtt ggtcgcgggg gtcttaagct
      421 cgaaggcgca ttacgctcat tcaatctcga tgttagcggt atgaattgcc tggacgttgg
      481 cgcatcaacc ggcggattta cggattgttt acttcagaat ggcgcgaaga aggtttgggc
      541 gattgatgtt ggtcataatc agattgactg gagattacga acagatcctc gagttgaagt
      601 tagagaaggc gttaatgctc gataccttca gcctgccgat ctcatggtca gttttgatct
      661 cgctgtaatt gacgtctcat ttatctcggt gaagcttgtg ttgccggcga tcgtcccact
      721 tctcaaggat gatggtcgag tgataactct gattaagcca cagtttgaag ttgggcgtgg
      781 acaggtcggg agtggaggta tcgttcgaga tgaagaacag catcgacgtg tggtcgctga
      841 ggtcaacgag ctagctgaaa gcatgggcct tcgtctggtc aacgtgatcg attctcctat
      901 tttaggtgcg gaaggtaaca aggagtttct tgcgctctat tcgaggtagt cctcactctg
      961 tgcaatcttt gtgtcctctg tgccttgtgg tggagcaaag tcctaagaaa accatcacca
     1021 aagagccaca gagaactcca gaggcagcgc agagttagga cactctatcc agaacggttc
     1081 gttgacacta aaaagatagc ctgcgtatca tcgccgctgc tttaattacc taacatcagg
     1141 gctagtcaca gttcaaccct gtggaaaccg cagccgctct gtgtctctgc ggtgaattcc
     1201 cctgatatcc aaagagatga acttgcatcg gaatacctaa gatcgcgtac aaacacggat
     1261 tcttttcaat catgtcctcc ataaaacgga taggcatttt catcaagcca catcaaccgg
     1321 aggtcttacg gaccatctgc gagctcacgc aatggctgac cgaacgaggt atcagcttag
     1381 ttggtggacc agaaattgat cgagaacgga ttgaacacga aacaggatgc cccgttgagc
     1441 aagttgacgc agataagctt gctgcatcgg tagatctatt cctggtgctc ggtggcgatg
     1501 gaaccatgat cgcgaccgcc aggttgatag gtgatcatga agtaccagtt ctaggcgtca
     1561 attatggagg tctcggttac cttgctgagt ttcgaattga ggaactctat ttggctctcg
     1621 aatccatcct ctcggataac taccgcctgg ataaaagagt catgctggct gttgaaatgt
     1681 accgcggcaa agaacccgcc atacacaatc gcgtcttgaa tgatgttgtg atcaataagt
     1741 cagcactggc ccgcattgtt gagatcgaag cctacttgaa tcagcaattt gtcaactcgt
     1801 tccgtgccga tggcctgatc atttctactc cgacaggctc gactgcctat aatctttccg
     1861 ccggtggtcc agtcatattt ccctcaatga atgcagtcgt catcactccg atctgtcctt
     1921 ttacactttc taacaggcct atcgttgtgc ccgacgacgc gctgatcgaa ctgcgtttga
     1981 agactgaaca agaagacgtg tcgttaacgt tggacggcca ggtcggcctg cctatgaaga
     2041 ctgaagatct tgtcgtcatc cgcaaaagca agacgacttt caacctcatt caaccgagca
     2101 acagaaacta tttcgatgta ctccgagata agttgcgctg gggccgttaa aatcagacca
     2161 cgaccgagtt cgattactta tgcctgaaga caacaataaa cgtttctccg gacagcttga
     2221 tccggccggt tttgaacaca aaacaacaac tgatcttgac gaagagactc cagacaagcc
     2281 taaaggtctg gcgcttcatc accgcatact gatcggactc gcggtcggag ttattgccgg
     2341 tatcactgtc aaccattttt tcggcggaga gaacccgaag gttgtctggg tcatcgacaa
     2401 cttcacgcag cccgttggac agctttttct ccgactattg ctgatgatcg tcgtgccact
     2461 tgtgttttct tctttagtcg ttggtgttgc aggcatcgga gacattagaa agcttggcag
     2521 agttggtctc aagtcgtttg cctattgcct ggttatatct ggaatttcgg caatcattgg
     2581 aatcacgctc gccaacacga ttaagcctgg aaagcgcatc gacccaagca catcggcggc
     2641 tcttcagcaa cgttacgcaa cggatgcttc gcgtcaggtt gatgacgcaa agaaaactag
     2701 tgctcaagcc caaaagccat taatggctgt agttgagaca atcgttccca agaacccgtt
     2761 ttatgcagta gctgggaaag aagatcccaa catgctgcac gtgatgtttt tcgcgttagt
     2821 gattgggatt gccataacgc tgattccagt ttccgttact ggcccacttc tacgcgtgct
     2881 cgaaggtctc tacgaagtca ctgcaaaaat aatcgagatg attatgaagt ttgcgccgtt
     2941 tgcggtggcc tgtttgctct tcaataacac tgcaaggttt ggactggatc tcctccaggc
     3001 tctggcatgg ttcgtcgtaa cagtgttgct tggcctttcg cttcacatgt ttggcgtgta
     3061 ctcgttatcg gtctactttc tttcgcgcat ttcgcccttt gaatttttca aacgaattcg
     3121 aactgtaatt ctgactgcgt tttccacttc gtcttccaat gcgacgcttc ccacggctct
     3181 gcgcgtgtcg gagcaaaatc ttggagtgcc gcaggaaatt aacagcttcg tgctaacagt
     3241 tggggctaca gctaatcaga acggcactgc attgtacgag ggtgttaccg tacttttcct
     3301 cgctcaactg gcgggtgtag atttatcgct ggggcaacag ttgatggtgg tatatctggc
     3361 gattcttgga ggggttggta ccgctggagt tccttcaggc tccattccgt tcatcatcgg
     3421 cgtgctggta acgattggag taaatcccgc tctgatcgca atcatactcg gcgtcgatcg
     3481 cattctggac atgtgtcgaa caactctcaa tgtggcagga gacatcactg ctgctacttt
     3541 tgttgctcgc tcggaaggtt acgaattgtt gcgaccacat gaaccggcga tcgtgcatgc
     3601 gggtgtcgcg atggatcgcg cattggattg attctttggc aacaacgccg taacaatggg
     3661 cgtataaacc tttcttaacg aaagtatccg ttacggagac gagatggttt taccgctgta
     3721 cgacgacaat tcggatcgca ccatcactcc agttgttaat tatgcgatta tcgcggttaa
     3781 cattttcgtc ttcgtgttcc tgcagcagtt gggtgcaaat caacaattta cgtacgcgtt
     3841 ctcaaccgtt ccgctggaga ttcttaccgg ccatgatgtt gtgactcaga gtcgagtttt
     3901 gttggatccg gtaagtggcc aacgagtgtt ggtaccaggt ctgggggaaa cccccttttc
     3961 cgtttacatc acactgatta cttcgatgtt tatgcatgga ggcatcgctc acatcgcggg
     4021 caacatgctg tttctgtgga tctttggcga caacattgaa gatcgactgg gccacgttcg
     4081 ttatctggtg ttttatctcc tctgcggcgt gcttgcgtcg ctggctcacg tgtttaccac
     4141 tgccgcgttc gccggtggaa atgagaatag tttgatcgtt ccgagtctcg gtgcgtcggg
     4201 agcgatctct ggagtactcg gtggctacat cttgttacac ccgactcgtc gtgtgacggt
     4261 gatcttgttc cgttttctga ctgatgttcc agcctatgtc gcgatcggga tctggttcgc
     4321 gtttcagctg atcagcgggc tagggatgtt gggtggtggc tcacaacagg gtggagttgc
     4381 ttacgcagct cacgtcggag gcttcgtggc gggtcttgtg ttgataaagc tcttcgacat
     4441 gggccaccgt cctcgccagt tgcccggcta ttagtaggtt gtggtttcgc ccaaagcgcg
     4501 ggttgggcgc gagaatcaac ccccaattgc tgcctaactt acttctcagt taacattcac
     4561 ctttgttaag gagaagtatg actacactcg taccgccacc aaatcagcag gaactcgata
     4621 tccaatctac tccgcgccag gagatgcgac tctttccgct cgacactttt ccatatgagt
     4681 ttcccggcaa agagataagt atcaatttcg agataacaga atttacagcc atctgtccct
     4741 tctcggattt tccagacttt gccacgattc gcatggaata cacgccaaac gaacgatgta
     4801 tcgaacttaa gagccttaag ctttatataa actcatttcg cgacatcaag attttccacg
     4861 aacacgttat caacgtaatc cttgaggact ttgtggctgc ttgcgacccg ttacgcgtgt
     4921 acatcgaagg cgattttcac gttcgaggca atatcaaaac tattgtcaag gccaactacg
     4981 aacggaatta aggcgctctc gatctaatgg accacacctt tctttactca attctcgctg
     5041 gtatcgtttt tgtattcatc attgtcgctg cgcgactggc cttccgttgg ttagtacgaa
     5101 tagcgctggt tgggataatt ctcctggttg tagtcggcag ccttgcgtgg tggtggttcc
     5161 gagggcccgc cagacctcaa actgaaacac gtcctactac gtcaagacgt cgatgacgga
     5221 ccggtaacaa tggcggacac cgataccgac gctgccgatc aatacgacgc tagtattgag
     5281 aacatcaaac taggcgaaag tactcccggt ttgtggcagc gataccggcg gttaccacga
     5341 aatgttttcg cgatcagcct ggtcagcctc cttaacgacg cctcaagcga aatcatctac
     5401 ccactgttgc cgatcttcct tgtctctacc ctcggcgcg
//
LOCUS       DASSFP010000265         6680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_373559, whole genome shotgun sequence.
ACCESSION   DASSFP010000265 DASSFP010000000
VERSION     DASSFP010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373559"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFH91_05840"
     CDS             <1..753
                     /locus_tag="VFH91_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862545.1"
                     /translation="FENEHHVMANLASMLSVGGVLFILEGERPEVGSDTEEELVEVMK
                     RYGTLESPFDYAYLRQLLDENGFLVIGDYASINGLFERETIVDNTLPLKGLALNYHYL
                     ACKKVTEGALASTVPDSKRPGLLRAQIEVVNSGPMISKAGKPLNIELQIQNTGDTLWL
                     AGQETRLGIVMPGVRIFDDNGTMITEFHGEPPLPRAVAPREKVRLKIDHPAPLRSGYY
                     TIKVDLVDQHICWFEDVGSEPLTIEFEVEKEF"
     gene            complement(762..1544)
                     /locus_tag="VFH91_05845"
     CDS             complement(762..1544)
                     /locus_tag="VFH91_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6862546.1"
                     /translation="MEIPDKSSANTPDNYRHIERLEDCFFYHTMDVPGFGEVRGHWDL
                     RGRFKEYIGGVDLNGKSVIDIGAATGFLSFESEKAGASRVLSFDIKNASQQTFLPFKD
                     KLYFRDHAKWARQYDTELEQWKNAYWLCHRLLNSKAEVYYGNVYEIPSEVGLFDVAIV
                     GSVLEHLSDQISALASISRLTKETIVIVSPLLDIDERIAQFGALAKHPENDFTWWIYS
                     KGVYREVLKMLGFNIVSVTSGEYYYHHGERFERRYTLVATRD"
     gene            complement(1565..4525)
                     /locus_tag="VFH91_05850"
     CDS             complement(1565..4525)
                     /locus_tag="VFH91_05850"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET6862547.1"
                     /translation="MNRSLSPRFSFSTDKLAAAAITLAPVLYFLPALLAHLVLGPDDG
                     VLFNIPLRVTTANLLRAGELPIWNPYIFGGMPLFATAQGGLLFPLNWIYLLFSPAVAT
                     NMMVIATYMVAGLGAYLFARKTGSSVAGAVVTGLIWQMGGFLVAQISHINIAHTAALL
                     PWTLWSLERYVETGSRRRGALLAVLVAVQIFAGHQQTFAYTFLLFCVYAIVMAFGNER
                     TRKRYLLSLGFAIAGVMLAAIQIVPTFELLENSIRSEATYDFFSSFSMPPSFIATYLA
                     PYIMGGGDGRLFRAPYIGQAFYTEYVSYASILGVMLAVTALLLKPDRRTRLWAAVVIA
                     GLALAFGRFLPLNVYRVVYYVPILNLFRVPARHLVEVHFAVAMLAGRGLTAISWSPNV
                     ERVKYRVATVAIGVFLLTWSIVTWLRPASFRLVRDAPVTLLRAPELFMPLVFATLSAW
                     AIWAFANRWRGATASLVAVLALDLFLWGQFSGWYPSAKRIPAEYWGTPESVELLRSIA
                     PADRSSYRILTSHQSFKPNSSVHTSGWLLWTEPDIYSMFGISNVAGYDGFGLERYSEL
                     AGQMKLWGELTDPNATLRSNSREIDLLNARYLIARHDDAPEPIVNADDVAPPSAFPDA
                     TETYANLMFSREDLALSNINSRKLLRLRVPPVKVDHIAIVSNLSWADQVPDGAAVAHL
                     KLNARDGRVFEFDLRAGTDTADWSYDRPDIRARIKHSRAAVATSFDLSDSQYKYEGHT
                     YLASFALPDKAIIESGQISIDHRDDSPDLFLSISHVSLIDTKEQKTYAMGRDMFTIEN
                     TVDTSGSRVNTDRWRHVAKGRHFDIYENGRALPRAWMTSDVQVLNGALILQVIRTGVL
                     PDGSKWDPLRTALVESAVPGGPTNSRETGKVTIKSYGASRIDLSAEATNNSFLVLAEN
                     DYPGWRAYIDGQSTEVIRTNYALRGLVIPSGQHQIVFVYRPWSIIGGLLVSLLTAAGL
                     ILIVRRKPNSQLQT"
     gene            4648..6297
                     /locus_tag="VFH91_05855"
     CDS             4648..6297
                     /locus_tag="VFH91_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET6862548.1"
                     /translation="MSLQFVVAISRKSITNDETAHIPAGYYHLVAGDFQLNTEHPPLV
                     KMWAAIPLLFIQPREPKSPENINDDFAQRSAVVEQQFWFANQTQFLRIGFWTRLMMIP
                     LTVLLGVVIFLFTRALSNSTAALVAVFLYSLEPTVLAHGRVVHTDLPAALGYLLFFFA
                     LFQYHQAPTQRRAILLGIVTALALLTKYSMVILFPLLVLYTLLLVWSNRRDSVWLKKL
                     LIHVVTVTAIVILIINSVYFFRHDQLSDPDRRWLVSTSQPYSGAIDHSVAALGHVIPT
                     YYLFGVYKVAIHNHYGHPAFLLGHYSNDGWRYYFPVAFALKTTMPFLILSLLALAWAI
                     FQIAARRRINLLWVLVPFLIYLAVSISGSINIGIRHFLPAFPFLFILGGIVLSEIMKS
                     PRRNVGIGVTVVLIGWMSFEVVRAFPNYTPYMNQLASGPNWRYLSDSNVEWGDDVNTL
                     AAYLKSHGERRVRGALSAGWITLPLYGIEYVSLAPPVEQQVDTRYVAIGASFLNGSTV
                     PSFEVDGKLISDSERVNLFQSYRNRTPEAVFGGSIYLFRER"
     gene            complement(6311..>6680)
                     /locus_tag="VFH91_05860"
     CDS             complement(6311..>6680)
                     /locus_tag="VFH91_05860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6862549.1"
                     /translation="RIVLTEYLEDEDLRELYCSCKIFVYPSLYEGFGLPPLEAMACGA
                     AVIASRIPTLVETMGDAAILVEPKNIAELAEAILGLLSDTEAAQELAKTGLERASQFS
                     WYLAALKTMAVYEHVLGRNQ"
BASE COUNT         1700 a         1682 c         1653 g         1645 t
ORIGIN      
        1 ttcgagaacg agcaccatgt gatggccaac ctggcctcga tgctttcagt gggaggcgtt
       61 ctgtttattc ttgaaggtga aagacctgaa gttgggtcag ataccgaaga agagctggtg
      121 gaggtgatga agcgctacgg cacactcgag tcgccatttg actacgctta cttacgacaa
      181 cttttggatg aaaatggatt tctcgtgatt ggtgactatg ccagtatcaa cggactgttc
      241 gagcgtgaaa caatcgttga caacacgctg cctttaaaag gacttgcgct caactatcat
      301 tacctcgcct gcaaaaaagt tactgaaggt gcgctcgcgt caaccgtccc tgacagcaag
      361 cgtcccgggt tgttacgtgc tcaaattgaa gtagtcaata gcggcccaat gatttcaaag
      421 gctggcaaac cattaaacat tgaactgcag atacaaaata ctggcgacac tctgtggctc
      481 gcagggcaag aaacgcgact gggcatagtg atgccgggag tacgaatttt tgacgataac
      541 ggaacgatga ttaccgaatt tcacggtgaa cctcccctac cgcgggcagt agctccgcgc
      601 gagaaagttc gactgaagat cgatcatcca gcaccgcttc gctcgggtta ttacacaata
      661 aaagtagacc tggtggacca gcatatttgc tggtttgaag acgttggatc agaacctctc
      721 acaatcgaat ttgaggttga aaaggaattt tgacgaagaa atcagtcccg agtggcaact
      781 agcgtgtaac gtctttcgaa acgttcacca tgatggtagt aatactcgcc gctggtaacg
      841 ctcacgatat tgaatcccag catttttagg acctcacgat aaacaccttt tgaataaatc
      901 caccacgtga agtcattttc gggatgcttt gccagggcgc cgaattgagc tatgcgctcg
      961 tcaatgtcca ataacggaga aacgatcacg atcgtttctt tcgtgaggcg cgaaattgag
     1021 gcaagtgcgc taatttgatc gctcaggtgt tcgagcacgg agccaacaat cgcgacatca
     1081 aacaagccca cttcagaagg gatttcgtag acgtttccgt agtaaacctc agccttcgaa
     1141 ttaagcagtc gatgacaaag ccagtaggcg ttcttccatt gctcgagttc ggtatcgtac
     1201 tgcctggccc acttcgcatg atcacgaaag taaagcttat ctttgaacgg aagaaaggtc
     1261 tgttgactgg cgttcttgat atcaaatgaa agcacgcgcg aagcaccggc tttttcagac
     1321 tcaaaactga gaaagcctgt tgcggctcca atgtcaataa ccgactttcc attcagatcc
     1381 actccgccga tatattcttt gaagcgtcca cggaggtccc aatgtccgcg cacttcccca
     1441 aaaccgggga catccattgt gtgatagaag aagcagtctt ccagtcgctc aatgtgacgg
     1501 taattatccg gcgtattcgc cgacgatttg tcaggtattt ccatgtcaga cagccgcagt
     1561 gcgttcaagt ttgcaactga gaattgggct ttcgccgaac tatcaaaatt agcccggccg
     1621 ctgttagcag tgatactaac agtccaccaa taatggacca cggacggtaa acgaagacaa
     1681 tctgatgttg accggacgga atcactaaac ctcgcagagc ataattcgtt ctgatcacct
     1741 cagtcgattg accgtcaatg tacgcgcgcc aaccgggata atcattctcg gccaaaacca
     1801 aaaacgagtt gttagttgct tcggcagaaa gatcgattcg actggctccg tagcttttga
     1861 ttgtaacctt cccagtctct cgcgagttgg ttggaccacc cggtaccgct gattctacca
     1921 acgcggtccg aagtggatcc cattttgatc cgtcgggcaa aacacctgtg cgtattacct
     1981 gtagaattaa agcaccattc aaaacctgta catccgatgt catccaggca cgcggcagtg
     2041 cgcgcccgtt ctcataaatg tcgaagtgtc gtccctttgc cacgtgacgc caacgatcgg
     2101 tgttcactct actaccagat gtatcaaccg tattttcgat ggtgaacatg tcacgaccca
     2161 tagcgtatgt cttctgttcc ttcgtatcta tgagcgacac atgagaaatt gatagaaaga
     2221 gatccggcga atcgtctcgg tgatcgatac tgatttgacc actctcgata atcgctttgt
     2281 ccggaagcgc gaaagaagcg agataagtgt gaccttcata cttgtattgt gaatcgctta
     2341 aatcgaagct ggtcgcgacg gctgcccgac tgtgtttgat tcgcgcacga atgtccgggc
     2401 gatcatagga ccagtctgcg gtgtcagtac cggctcgcag atcgaattca aaaacgcggc
     2461 catctcttgc gtttaattta agatgcgcta cggctgcacc gtcagggacc tgatcggccc
     2521 aggacaggtt acttacaata gcaatgtggt ccactttgac gggaggcact ctaagacgaa
     2581 gaagcttcct tgaattgatg tttgataatg cgagatcttc gcgtgaaaac atcaaattag
     2641 cgtaagtctc agtggcatca ggaaaggctg agggtggtgc aacatcgtcg gcgttgacga
     2701 ttggttccgg cgcatcatca tgcctggcaa ttaggtagcg agcgttaagc agatcgattt
     2761 cgcggctatt actgcgaagg gtggcattgg ggtcagtcag ctctccccat aacttcattt
     2821 gaccagccag ttcgctgtaa cgttcaagtc caaatccgtc ataaccggct acgttcgaga
     2881 tgccgaacat tgaatagatg tccggctcag tccagagcag ccagccagag gtatgtactg
     2941 aggaattggg cttaaacgat tgatggctcg tcaggattcg ataactggaa cgatcagccg
     3001 gtgcgattga gcgcaacaac tctacgctct ctggtgtccc ccaatattct gcggggattc
     3061 gcttagccga tggataccaa ccgctgaact ggccccacaa aaataagtca agcgccagca
     3121 ccgctactaa actcgccgtg gcgcctcgcc accgatttgc aaaagcccag atggcccatg
     3181 cactcaacgt tgcgaatacg agtggcatga aaagctcagg cgcgcgcaag agcgtaacgg
     3241 gagcatcgcg cactaaacga aaactggctg gtcgtaacca ggtgactatg gaccacgtta
     3301 acagaaaaac accaatcgct acggtggcga cgcggtattt caccctctcc acgttcggtg
     3361 accacgagat tgctgttagc cctcgaccgg cgagcattgc tactgcgaaa tggacttcca
     3421 cgaggtggcg cgccggaaca cgaaatagat tgagaatcgg cacgtagtag accactcggt
     3481 acacattcag cggcaggaaa cggccaaagg cgagcgccaa acccgcgatt actactgctg
     3541 cccaaagcct ggttcttcga tcaggtttta gaagcaacgc agtgacagcg agcatgacgc
     3601 ctaaaatgga ggcgtacgaa acatactcgg tgtagaaagc ttgaccgatg tagggtgcac
     3661 gaaatagccg tccatctccg ccacccataa tgtagggcgc aagatatgtc gcaatgaatg
     3721 aaggcggcat cgaaaacgag gagaagaagt cgtaagtggc ctcagagcga atgctgtttt
     3781 caagcaattc aaaggtcggc acgatttgaa tcgcagccag catcacgcct gcaattgcga
     3841 aacccagcga caaaagataa cgtttgcgag ttctttcatt tccaaaagcc atcacgattg
     3901 cgtacacaca aaataatagg aatgtataag caaacgtctg ttggtgaccc gcaaatattt
     3961 gaaccgccac cagcacggcg agtagagcac cgcgacgccg acttccggtt tcaacatatc
     4021 tttccaggga ccaaagggtc cacggtaaca atgctgctgt gtgtgcaatg ttgatgtggc
     4081 tgatctgggc tacgagaaaa ccacccattt gccagatgag accggtgacg actgctccag
     4141 caactgaact tccagttttt ctggcaaaca agtaggcgcc caacccggca accatgtagg
     4201 tcgcgatcac catcatgttg gtagccacag ccggtgagaa aagtaagtaa atccaattta
     4261 gcggaaagag taagccgcct tgagccgtag caaacaaagg cattccaccg aatatgtagg
     4321 gattccaaat tggcagttcg cccgctcgca gaagatttgc agtcgtaact cgcagcggaa
     4381 tgttaaaaag cacgccgtcg tctggtccaa gaaccagatg agccagtaga gcgggtaaga
     4441 aataaagaac tggtgcgagc gtaattgccg cagctgcaag cttgtcggta gagaaactga
     4501 agcgaggaga tagtgagcga ttcattgaat caacaggccg cagacccggt tttcatctct
     4561 ctcgagccgc gtcgactttt gtcgcaactc tggatttggg tactgtcgcg taaatttgag
     4621 gttctttgcg gagttctact tctcacaatg agcctgcagt ttgttgtggc tatttcgcgc
     4681 aaaagcatta cgaatgacga gactgctcac attccggcgg gatactacca tctcgtcgct
     4741 ggagacttcc aactcaatac tgaacaccca ccactcgtta agatgtgggc ggcgataccg
     4801 ctgctgttta tccagccaag agaaccaaag tctcccgaga acattaacga tgacttcgct
     4861 cagcgatctg cagtagtaga acaacaattc tggtttgcaa atcagactca gtttttgcgc
     4921 attggattct ggacccgcct gatgatgatt ccactgactg tactgctcgg agtcgttatt
     4981 ttcctgttta cacgcgcgct atcaaactcg acggctgcgc ttgtcgcagt cttcttgtac
     5041 agcctcgagc caactgtcct ggcccacggt cgcgtagtac acacggatct acctgccgcg
     5101 cttggttact tgctcttctt tttcgcgctc tttcaatatc atcaagctcc cacgcaacgg
     5161 agggcgatcc tactcggcat cgtcacggca ctcgccctac tgacaaaata ttcgatggtt
     5221 atcctcttcc ctttgctggt gctatacact ctgttgctgg tgtggtccaa ccgtcgagat
     5281 tccgtttggc ttaagaagtt gctgattcat gtcgtgactg tgactgcaat agtcatatta
     5341 ataatcaatt cagtttactt tttccggcac gatcagttga gtgatcctga tcgtcgctgg
     5401 ctcgtaagta cgtcgcaacc ctattccggg gcgattgatc acagtgtcgc cgctttaggt
     5461 cacgttatac caacgtacta cctgtttggt gtgtacaagg ttgcgattca caatcattac
     5521 ggtcatccgg cttttctgct gggacactat tcgaacgatg gttggcgtta ctattttccg
     5581 gtcgcgtttg cgttaaagac gactatgcca tttctcatcc tgagtctact agcgttggcg
     5641 tgggccatct ttcaaattgc cgcacggcgc agaatcaatc tactttgggt gctcgttcca
     5701 tttttgattt acctggcagt ctccatttcg ggctcgatca atatcggcat ccggcacttt
     5761 cttccagcat ttccattttt attcatcctc ggcggaatag tgctctcaga aattatgaaa
     5821 tcgcctcgcc gcaacgtcgg cattggtgtc acggtggtgc taattggctg gatgtctttc
     5881 gaagtggtgc gtgcctttcc aaattacacg ccttacatga atcaattagc gtcgggtcca
     5941 aattggcgat acctctctga ctcaaatgtg gaatggggcg atgacgtcaa tactcttgca
     6001 gcttacttga agagtcatgg tgagagacgt gttcgagggg cactgtcggc aggttggata
     6061 acgttgccac tatatggaat cgagtacgtc agtctcgcgc ctccggttga acagcaagtc
     6121 gatactcgct acgttgcaat tggtgcgagc tttttaaatg gatctactgt tccgtcattt
     6181 gaggtagatg ggaagttgat aagtgacagc gaacgcgtga atctctttca gtcttatcga
     6241 aaccggacac ctgaagctgt ttttgggggg agtatctatt tgtttaggga gcggtgagtc
     6301 cccatatttt tcattggttc ctccccagaa cgtgctcata gaccgccatg gtcttcaaag
     6361 ctgccagata ccacgagaat tgagacgccc gctcaagtcc cgttttcgct agttcctgag
     6421 cagcttctgt atcacttaat agtccaagaa tagcctcagc gagttcggcg atatttttgg
     6481 gctcaaccag gatcgcggca tcacccattg tttcgactaa cgtggggatg cggctagcga
     6541 taacagctgc gccacaagcc attgcttcca gcggcggtag tccaaagccc tcgtacaacg
     6601 acggataaac aaaaatcttg cacgaacaat agagctcccg cagatcctcg tcttctaaat
     6661 actccgtgag aacaattcga
//
LOCUS       DASSFP010000266         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_71892, whole genome shotgun sequence.
ACCESSION   DASSFP010000266 DASSFP010000000
VERSION     DASSFP010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71892"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..581
                     /locus_tag="VFH91_05865"
     CDS             333..581
                     /locus_tag="VFH91_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862550.1"
                     /translation="MPARSKHSAKVICYQGNVWISPRQFWNLVRQNLIEYLSEPPLTG
                     RFKGQASDFLITINHTILSMACPEHRSEVLFSKRYAKK"
     gene            609..1283
                     /locus_tag="VFH91_05870"
     CDS             609..1283
                     /locus_tag="VFH91_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6862551.1"
                     /translation="MLELIQPISKRDQVVRAMKDAILSGAIQPGASLVESKIAQQLGA
                     GVPLIREALIELEHQGFVQRTPYKGTTVTTLSPVDVRNIFALRVELEALAIEWARANI
                     TDADVQYLREVIHKMEDAAQVLDLPRFYESDLAFHRKIWSLSGNPYLAHTLERLVVPL
                     FAFFLMKTSRHQDSYIESACTHSKIVEAIPTATPAELRDIFKASLVSWKDDMVQLLFS
                     REYGPS"
     gene            1270..>2395
                     /locus_tag="VFH91_05875"
     CDS             1270..>2395
                     /locus_tag="VFH91_05875"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6862552.1"
                     /translation="MDHHNSNSEVILRLSDIVKSFDGVAALKGVSFELREGEVHALVG
                     ENGAGKSTLVKTITGAVVPDSGAIEFDGKRISDHSPAYAKTLGIAAIYQQPALFPELT
                     VTENISLGIEKPKPWRRIDWNARRTRAVELLQRVGARIYPECDVQSLSMPEQQLVEIA
                     RALGANARVLIMDEPTASLAEEETRNLFRVIDALKKNGVAIIYISHRLEELEAVADSV
                     TVLRDGAIVDTRMMSEVSRNELIRLMVGRELSAVFPKREVGIGKAFLETRGLSCRLNG
                     VENIDLTVRAGEIVGLAGLVGAGRTQLARTIFGLEPADRGEIRINDQTVRIDKPSTAI
                     QLGIAYLPEDRRRHGVMLDMPIDANVTLASLDKLRKGLTFD"
BASE COUNT          603 a          578 c          631 g          583 t
ORIGIN      
        1 acggagtggg actgtctttg gggagttggc ggctctaaac atgtcacccc aaccgggggt
       61 gaaacccaat gctcttccca tttgctaaca aatcggctct tgctttttcc agggagattc
      121 tttcgcagca ttgctcaaag gccaccgcat gacttcgcta gattgttaat tcgaacctta
      181 gaacaagtag gccttgaaaa aacctaaacg aataaccaga tcataatatg atcttgtaat
      241 ctgcagaaga tgaagtatac tgcgcgcgtt taaacacagc ctgctccggt aattggagat
      301 tcccaatcag ggcgaagtgc ggaggtgttc gtgtgcctgc cagaagcaaa cactcagcaa
      361 aagtcatctg ttatcaggga aatgtttgga tttcaccgcg ccagttttgg aatctcgtgc
      421 ggcaaaatct gattgaatac ctcagcgagc cacctctaac cggccgattc aagggacaag
      481 cgtcggactt tttgattacg ataaatcaca cgatacttag catggcctgt cctgagcacc
      541 gttcagaagt cctgttttcc aagcgttacg ccaaaaaata accctaagag cacctgtaag
      601 aagcgagtat gctcgaatta attcaaccca tctcgaagcg agaccaggtt gtccgtgcca
      661 tgaaggatgc gatcctttca ggtgcgattc aaccgggagc atctctggtt gaaagtaaga
      721 ttgcacaaca gcttggcgcc ggagttccgc taatccgaga agcgctgatt gagcttgagc
      781 accagggttt cgtccaacga acaccatata aaggcaccac ggtaacgaca ctctccccgg
      841 ttgacgtcag aaacatcttt gcgcttcgtg tcgaacttga ggcactcgcg atcgagtggg
      901 cgcgagccaa tatcacagac gcagatgtgc agtatctgcg agaggttatt cataaaatgg
      961 aagacgcggc tcaagtgctt gatcttcctc gtttctatga gagtgacctc gcttttcacc
     1021 gaaagatttg gagcctctcc ggtaatcctt atctcgctca tactcttgag cgtctggttg
     1081 ttcccctgtt cgcatttttc ctgatgaaga ctagtcgtca ccaggactcc tatatcgaga
     1141 gcgcctgtac ccactcgaag attgtcgaag caattccgac tgctacgccc gcggagttgc
     1201 gcgatatctt caaagcttct ctggtgagtt ggaaagacga catggttcag ctgctgtttt
     1261 ctagagagta tggaccatca taattccaac tccgaagtga tactgcgact tagcgatatc
     1321 gtgaagtcat tcgacggcgt cgcggccttg aaaggagtct ctttcgagtt gcgtgagggc
     1381 gaggttcatg ctcttgtcgg cgaaaatggt gcgggcaaat cgaccctggt taaaacaata
     1441 accggtgcag tcgttcccga tagtggtgca atcgaatttg atggtaagcg tatctccgat
     1501 cattcaccgg catatgccaa gacgcttggc atcgccgcca tctatcaaca acctgccctg
     1561 ttcccggaac ttacagttac ggaaaacatc tcgctcggga ttgaaaaacc gaagccctgg
     1621 cgacgtattg attggaacgc aagacgaacc cgggcagttg agctgcttca gcgcgtggga
     1681 gcgagaatct atccggagtg cgatgtgcag agtttgagta tgcctgagca gcagttggtg
     1741 gagattgccc gagctttagg agcgaacgcg cgggtgctaa tcatggatga gccaaccgcc
     1801 tcgcttgctg aagaagagac gcgaaatctc tttcgagtaa tcgacgcctt gaaaaagaat
     1861 ggggtggcga tcatttacat atcgcaccga cttgaagagc ttgaggcggt cgccgacagc
     1921 gtcactgttc tccgtgacgg tgcaatagtc gatactcgaa tgatgtcgga agttagtcga
     1981 aacgagttga ttcgactgat ggttgggcgt gaactctctg ctgtctttcc caaacgcgaa
     2041 gtgggaattg gtaaagcctt tttggaaacg agaggtctgt cgtgtcgctt gaatggtgtc
     2101 gaaaatattg accttacggt tcgcgcaggt gagatcgttg ggctggctgg tctggttggc
     2161 gcagggcgta cacaacttgc ccgcacaatc ttcggacttg aaccagcgga tcgaggcgag
     2221 attcggatta atgaccaaac agtgcgcatc gataagccct caactgcaat tcaacttgga
     2281 attgcgtatc tacccgaaga tcgaaggcgg cacggcgtta tgctggacat gccgatagac
     2341 gcaaatgtta cgctcgcgtc gctggacaaa ctgcgaaaag gattaacgtt tgata
//
LOCUS       DASSFP010000267         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1846673, whole genome shotgun sequence.
ACCESSION   DASSFP010000267 DASSFP010000000
VERSION     DASSFP010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846673"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFH91_05880"
     CDS             <1..212
                     /locus_tag="VFH91_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862553.1"
                     /translation="PPAKTKLLRQFASDDEIIWRNDAIKDCSKSCLGLPAGTKLYEVD
                     VPAFHLQLAEIEGEMKIVSAMYSYQ"
     gene            complement(214..597)
                     /locus_tag="VFH91_05885"
     CDS             complement(214..597)
                     /locus_tag="VFH91_05885"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HET6862554.1"
                     /translation="MQEKHLFEYAVIRLVPCVEREEFINVGVVLYCARQEFLQMKFDL
                     NESRLKAFGCEVDISEIKDQLTGFERVCSGGTDAGTIGNLPMAARFRWLTAPRSTVVQ
                     TSPVHTGLTKDAAATLSELQAKLAS"
     gene            complement(575..1363)
                     /locus_tag="VFH91_05890"
     CDS             complement(575..1363)
                     /locus_tag="VFH91_05890"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HET6862555.1"
                     /translation="MSTEIRTINVTRYVTPLREGGSLPAIIEADDDGLYVLKFRGAGQ
                     GLKALVAELLSGEIARNLGLQVPELVFATLDRELSRLEPDPEIQDLLRASAGSNLAMD
                     FLPGSLTFDPVVETVDGKLASQIVWLDALVTNVDRTARNTNMLMWHKQLWLIDHGASL
                     FFHHAWKDWEEQSRRPFSQIKDHVLLRYATDLEAVDSEFSSRLTANVIAATVDLIPDE
                     WLADELQVRSVEEKRMVYKTFLQTRVEHSRVFVEEAQRAREASV"
     gene            1837..>2276
                     /locus_tag="VFH91_05895"
     CDS             1837..>2276
                     /locus_tag="VFH91_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6862556.1"
                     /translation="MNQEVVAANLAIVEAHFHTEAANEIEKALELYTEDIIWESPTRD
                     LLFQGKEATAENYRKMFASFKVEDFQTLQRFATETRVVDDSVLIAELIGEGVDNAPAP
                     VGSRVEIRLLHVFEMRDKKISRELVFENWRVVQPSVLYSTTTIDL"
BASE COUNT          590 a          570 c          545 g          571 t
ORIGIN      
        1 ttccgcctgc taaaaccaaa cttttgcgcc agttcgcaag cgatgacgaa attatttggc
       61 ggaacgacgc gattaaggat tgttcaaagt cgtgtttggg gctaccggcc ggcaccaaat
      121 tgtatgaagt cgacgtgccg gcgtttcatt tgcagttagc agagatcgag ggagagatga
      181 agatagtctc ggccatgtac tcctatcaat agtctaactg gcgagttttg cctgaagttc
      241 tgaaagagta gcggcggcat ctttagtcaa accggtatgt accggcgaag tttgcacaac
      301 tgtgctgcgc ggagcagtta gccatctgaa tcgagcggcc atcggcaggt ttccaatcgt
      361 ccccgcatcg gtcccgcccg aacaaactcg ctcgaagcca gtcagctgat ctttaatctc
      421 cgaaatgtct acctcacagc caaacgcctt caaacgactc tcattgagat caaatttcat
      481 ctgcagaaac tcttgccgag cacagtagag cacaacacca acgttgatga actcttcgcg
      541 ctcgacgcac ggaaccaagc gaattacagc gtactcaaac agatgcttct cttgcacgct
      601 gtgcctcctc aacaaatact cgcgagtgtt cgactctggt ttgtaagaac gttttgtaga
      661 ccattcgctt ttcctcaaca cttctaacct gcaactcatc tgccagccat tcatcgggga
      721 tcagatcaac ggttgccgca atgacgttgg cggtcagtcg tgagctgaat tcactatcga
      781 cggcttcaag gtcagtcgca taacgcagca agacatggtc ctttatttgg gagaatggtc
      841 tgcggctttg ctcctcccag tctttccagg cgtggtgaaa aaagagcgat gccccatggt
      901 cgatcaacca taattgtttg tgccacatca acatgttcgt attgcgagcc gttcgatcga
      961 cattggtaac aagagcatcg agccaaacga tttgcgacgc taatttgccg tcaacggttt
     1021 caacaactgg atcaaacgtc aacgagcccg gcaagaaatc catcgcgagg ttagaaccgg
     1081 cactcgcgcg aagcaagtct tgaatctcag gatcaggttc gagacggctc aactcgcgat
     1141 caagagtggc gaatacgagt tcgggcactt gcaaaccgag attacgggcg atctcacctg
     1201 aaagaagttc agctacgagc gctttcaggc cctgccccgc gccacggaac ttaagtacat
     1261 acaatccgtc gtcgtcagct tcgataatag cgggcagtga accgccttct cgcagtggtg
     1321 tgacatagcg cgtcacatta attgttctga tttctgtact cactttctat tgcgtgccgc
     1381 tttgattaag gccgtgatct ctttccgatt tacggtttct gccagaccga tcaccgcacc
     1441 atcctttctg acaactaatt ctggatggtg gtcaggcatg ttcaaagctc aacaggttgg
     1501 tccagcgcgt gacatgttac ggcagacccg ttcgcctgta tacattcagc tgccagatac
     1561 gagtcagtac cacaacgcgg tcaggaggaa atcattggat agttatgagt tgatatttga
     1621 tagtttctat tttgatctgc acctcgcttt cagcaaatag caattcttaa gtatcaactc
     1681 ataattatcc aatgatttct tcctgaccgc gttgtggtac tggctcgtat gggtcgccgc
     1741 agtggcccat ttcggagacc gaatccgcgt taagtaatag agaaaacagc acagaattta
     1801 tgagcaggac aagtgtcccg ctggagataa atgattatga atcaagaagt tgtggcagca
     1861 aatctggcta tagttgaagc ccattttcat accgaagcag cgaacgagat cgagaaagca
     1921 ctggagcttt atacagaaga cattatctgg gaatcaccaa cgcgcgacct ccttttccaa
     1981 ggtaaagaag ccaccgccga gaactatcgc aagatgtttg cctcatttaa agtcgaggac
     2041 tttcaaacat tgcaacgctt tgcgaccgaa acccgcgtag tggacgatag cgttctgatt
     2101 gccgaactaa ttggagaagg tgttgacaat gctccagctc cagttggttc gcgagttgag
     2161 ataaggctgt tgcatgtttt tgagatgcga gataaaaaga tctcacgaga gttagtcttt
     2221 gaaaactggc gagtagtgca accctccgtc ctttattcga ctaccacgat tgacct
//
LOCUS       DASSFP010000268         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1406666, whole genome shotgun sequence.
ACCESSION   DASSFP010000268 DASSFP010000000
VERSION     DASSFP010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406666"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1850)
                     /locus_tag="VFH91_05900"
     CDS             complement(177..1850)
                     /locus_tag="VFH91_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6862557.1"
                     /translation="MAHLKITDASGRQWQYSFLPQATCTIGRAPDNTVVLDDPRASRY
                     HAHIKQLDNGVFTIVDGAVIAGQLRRSANKVFINGEPEFEHQLKTGDRITIGASTLRF
                     EQPAEERTTDVRYDDKPLGHTQLLISANDVMSTVLRSSKEEIVALPSRDKVLESLQRK
                     ANILSALYEMSQTLGSVFDLNAIFAKATDVIFRSTPADRVVALLADAPSGANPEEVNL
                     TPIAMRARDDKLEAHARKLSIGRTITRKVMKDRVALLSQDAAADEEFAGVDSIVSQGV
                     RSTICAPLMAETRVHGALYADRLDPFAAFKPDDLELISAVAAQTAVAVENARAHERLA
                     REEVARANYSRFLPEYVVKQMLDNPDSFKLGGLNQTITILFADIRGFTRISEHAPPEK
                     IVSLLNRYFSAMTDIIFAHGGTLDKYLGDGLMALFGAPTATPDDASNALNAAVAMQRR
                     LLGINQELEAEGLPSIGVGMGLHTGSVVVGYIGSERRSEYTAIGDPVNTSSRLESNAR
                     GGEILISDATAKAAHSRYKLLPREPIMVKNRQQPVVLWEVDWQRASGAF"
     gene            2002..>2359
                     /locus_tag="VFH91_05905"
     CDS             2002..>2359
                     /locus_tag="VFH91_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET6862558.1"
                     /translation="MRSSTLVCLISVAMALVAADLRAQTKQTLRSTISGQVTIKGKPI
                     PGLVVGLRGTDGVMLNTFEVLPRATTDQDGNYRITNVSPGTYAIQPAAPAYVLTSPLD
                     PSGRSVIVGEGETIENI"
BASE COUNT          575 a          634 c          588 g          562 t
ORIGIN      
        1 atgaatgatc ctgttcggcc aataaaagac ataagctaag tccacaaaat cggtcagttt
       61 aaactgactg cagttcaaaa acagagaggg tgaacaagaa cggaccacca catttgcccg
      121 ctctcattca cctcccaaaa cctcccccaa aaactgctgg ctactgctat aaaatgtcag
      181 aatgcaccac ttgctcgttg ccaatcaact tcccaaagga ctactggttg ttgcctgttc
      241 ttaaccatga tgggctctcg tggaagcaac ttatagcgac tatgagccgc ctttgccgtg
      301 gcatcgctga tcaaaatctc tcctccgcgc gcgttcgatt cgagacggga agatgtattc
      361 actgggtcac cgatagccgt gtattcggaa cgtcgctcag aaccgatgta gccaacgacg
      421 acgcttcccg tatgtagtcc cattcccaca ccaatcgagg gcaacccttc agcctctaac
      481 tcctgattaa tcccaagtaa tctccgctgc atagcgacgg ctgcattcag agcattagac
      541 gcatcatccg gggttgcggt tggagcacca aagagtgcca tcaacccatc accgaggtat
      601 ttatcaagcg ttccgccgtg agcaaaaatt atatcggtca tcgccgaaaa gtagcgattc
      661 aataaactga ctatcttctc aggcggcgcg tgctctgaaa ttcgagtgaa gccccgtatg
      721 tcggcaaaaa gaatggtgat ggtttgatta aggccaccga gcttgaacga gtcaggattg
      781 tcgagcatct gcttaacaac gtactcggga aggaaccggc tgtagttagc gcgtgcaact
      841 tcttcacgcg cgagccgttc gtgagctcgg gcattttcaa ctgccaccgc tgtttgtgcg
      901 gccacggcac tgatcaattc caagtcgtcg ggtttaaatg ctgcgaaagg atccagccgg
      961 tcggcataaa gagctccgtg cactcgcgtc tcagccatta acggtgcgca aattgtcgag
     1021 cgaactccct gagaaacgat cgagtcaaca cccgcgaact cttcatcggc cgcagcatcc
     1081 tgtgaaagaa gtgctacccg atctttcatc accttccgtg tgatggttct gccgattgag
     1141 agctttcgcg catgggcctc gagcttgtca tcgcgagcgc gcattgcaat gggcgtcagg
     1201 ttcacctcct ccggatttgc gccagaagga gcgtcggcca gcaacgccac aactcgatct
     1261 gcgggagtcg atctgaagat gacgtcggtt gcctttgcaa agatggcgtt tagatcgaac
     1321 acagagccta acgtctgact catttcgtag agcgcgctga gaatgtttgc tttccgttgt
     1381 aacgactcga ggaccttgtc tcgtgagggt aaagcaacga tctcctcctt cgatgatcgc
     1441 agcacagtgc tcattacatc attcgctgag atcaggagtt gtgtatgtcc gagcggctta
     1501 tcgtcgtagc gaacgtccgt tgtgcgttct tcagcaggct gttcaaaccg gagagtgctc
     1561 gctccaattg ttattcgatc gccagtcttt aactggtgct cgaattcagg ctcaccgttt
     1621 atgaaaactt tattggcact gcgtcgcagt tggccggcga tcaccgcgcc atcgactatc
     1681 gtgaagactc cgttgtctaa ctgtttgatg tgagcgtgat aacgagaagc ccgtggatca
     1741 tcaaggacca ccgtattgtc cggtgcgcga ccaatagtgc atgtagcttg aggcagaaag
     1801 ctgtattgcc attgacggcc actagcgtcg gtgatcttaa ggtgagccat gcgcgggtga
     1861 tcctaacagt agcaagttaa gctgacaacc tgcctaaact tccccggctc gaaactggcg
     1921 ctggcaaaat ctggtaagca caaatgcatt cagatatcat gcacccgcca gactcactta
     1981 gttgatagga gttccacccc tatgaggtcc tctactctcg tctgtctcat tagcgttgcc
     2041 atggcccttg ttgctgctga tctacgcgcg cagaccaagc aaactctgcg aagtaccatc
     2101 tcaggacaag tgacaatcaa aggaaaacca attccaggac tcgttgtggg tttgcgtggc
     2161 actgacggag tcatgctgaa cacctttgag gttttgccca gggcgaccac ggaccaggat
     2221 ggaaactatc gcatcaccaa tgtctctcct gggacatatg cgatacaacc tgcagctccg
     2281 gcttacgtat tgaccagtcc acttgatcca tcaggaaggt ccgttattgt aggcgaaggg
     2341 gaaacgatcg aaaatattg
//
LOCUS       DASSFP010000269         5986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2013395, whole genome shotgun sequence.
ACCESSION   DASSFP010000269 DASSFP010000000
VERSION     DASSFP010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013395"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFH91_05910"
     CDS             <1..310
                     /locus_tag="VFH91_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862559.1"
                     /translation="SDLRDHPGIPRLLKTNANGLRDGYRAALSTNPELKFGQYVSATR
                     VSQNLGRRFPNVTRSNILSGLASGRSLGQTLQDLGVGERDAKDAKKQAEREIKDARRN
                     "
     gene            638..1927
                     /locus_tag="VFH91_05915"
     CDS             638..1927
                     /locus_tag="VFH91_05915"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET6862560.1"
                     /translation="MSGNSLSITDNRTGKQYEVPIENGTIRAMDLRQIKVDNDDFGLM
                     TYDPAFMNTASCKSRITFIDGDKGILEYRGYPIDQLAEKSSYLEVAYLLLNGELPNQT
                     ELDSWTKNITYHTFINENIKKVIDGFHYNAHPMGMFEATLGALSTFYPDAKDIFNEEC
                     RWKQIYRLIAKVPTIAAFAFRHRIGMQYSYPDNDLSYEGNFLNMMFKTTELKYQPNPV
                     LERALTVLYILHADHEQNCSTNAMRSIGSAHTDPFSALSGAAAALYGPLHGGANEMVL
                     RMLKEIGSVQNVPDYIKRVKAGEFRLMGFGHRVYKNYDPRARIIKQVADEVFEVTGKD
                     PLLDIALELERIALEDDYFVKRRLYPNVDFYSGIIYQAMRFPVDMFPVMFAIPRTSGW
                     LAQWVELLQDEDQKIARPRQVYLGNRTRDYVPISSRG"
     gene            complement(2078..2896)
                     /gene="speB"
                     /locus_tag="VFH91_05920"
     CDS             complement(2078..2896)
                     /gene="speB"
                     /locus_tag="VFH91_05920"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET6862561.1"
                     /translation="MSKAPEHENTNLALVGFRYDENSSFMKGASEAPPQIRAALRSEA
                     WNMTSESGTDLSGETIFFDAGDVEAVPGTDMLSLVENSMVTLLNDGLKPISLGGDHSI
                     TYPIVRAFARKYRDLSILHFDAHPDLYDSYQGNKYSHASPFARIMENRLAKRLVQVGI
                     RSINSHQREQAQKFNVETIEMRDLRDGLQLTFDSPLYISFDVDVLDPAFAPGVSHREP
                     GGLSTRQAIDLIQRLKGSVVGADIVEFNPRMDPLHVTGTVCAKLLKEIAARMME"
     gene            complement(2958..3034)
                     /locus_tag="VFH91_05925"
     tRNA            complement(2958..3034)
                     /locus_tag="VFH91_05925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2998..3000),aa:Arg,seq:tcg)
     gene            complement(3278..3688)
                     /locus_tag="VFH91_05930"
     CDS             complement(3278..3688)
                     /locus_tag="VFH91_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6862562.1"
                     /translation="MKPIAIGIGCALLALTFSLTFLGCNNNTAATNPAPHSPTATSPT
                     APNSELASARINYEKKCAGCHGPTAEGGTAKVNDKQIKVPSLKSERAMKHDDKRLTET
                     ITDGDEEMPAFKDKLKTQEIADLVKLIRKEFQGK"
     gene            3972..4742
                     /locus_tag="VFH91_05935"
     CDS             3972..4742
                     /locus_tag="VFH91_05935"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET6862563.1"
                     /translation="MVSDKEQVNPPRGSLRLAPTSQEVEEQKAKLPRQFVNFTFYHAR
                     PDWRLLPDTEKQRCKDQFVKTVDEFRSSLLMHCYSTIGLRTNVDFMIWRIGYDLEPFQ
                     EMTARLNKTEMAKYIEPSQSFLSMTKRSMYIDKDNPEHVEDRLHIIPGKSQYLFVYPF
                     VKTREWYTRPAEQRQEMMDEHIRIGSKYRSVKLHTTYSFGLDDQEFVVAFETDHPADF
                     LDLVQELRETKASSYTLRDTPMFTCRQRTLAECLDSLG"
     gene            5005..>5986
                     /locus_tag="VFH91_05940"
     CDS             5005..>5986
                     /locus_tag="VFH91_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862564.1"
                     /translation="MTATHPAIVADLNITQTSKPQEGNSEYLWYEAENMRGFTTGKLG
                     EPIVNPSYLDLPKEKSPGWGMNGPGVSAEWTQGGESEWNSAAASANETNASIYQDLEV
                     PRSGKYKFWVRYADWANKNENFSVTISQNGQRVFQHEFGTKDIIDPHDEVSMYWGWSF
                     TWDGADTSLGKGPARVSIEIRKPAEARRNVDCFVLTNDLSYVPSNREKPDFAAMRYLR
                     QWSSSRVPLASIVQNDSEAIPDVWLRPKVANRDFMMPWNIAREFWAFYDQPEEQRPIY
                     PFNAETIGQFVERYKGVKEVPLFDSKFVVPVIYLNHVPELFQEGSAFVRYL"
BASE COUNT         1564 a         1433 c         1464 g         1525 t
ORIGIN      
        1 tagtgacctt cgcgatcatc ctggcattcc cagacttctt aagaccaacg ctaatggtct
       61 gcgagatggt tatagagctg cgttatctac aaatccagag ttgaagtttg gtcagtacgt
      121 ttcggccaca cgggtcagcc agaatctcgg aaggcgattt ccgaatgtca cccggagcaa
      181 catcctttca ggtttagcat caggacgcag tttgggccag acattgcagg atctaggggt
      241 aggtgaacgc gacgcaaaag atgcaaagaa acaggccgag cgtgaaatca aagacgctcg
      301 tcggaactga tagcatcaac tcctgagttt cacggaccac cgaagaccac cggcttcagc
      361 gaccgtgtga aaactcaaga atacaatcca cagattacgc cggttacaca gatttctttc
      421 agtgaaaggc atggcgcaaa ggcgttctag taaagaatct gtgtagtcgg cgtagtctgg
      481 gattgtattc ttgggtttca cacagtttct tcagccgcat ggttcatcaa cctttaccta
      541 gccatacctc cttaccgctt gagaagtcac agatgcatag gctaatctgc ttcgcataat
      601 aaataacagt ttcgatgaga ccccaaggaa gattacgatg agcggaaatt ctctcagcat
      661 cactgacaat cgcactggca aacaatacga agtaccaata gaaaacggga cgatcagggc
      721 aatggacctt cgtcaaatta aagtcgacaa cgacgacttt ggtttgatga cgtacgatcc
      781 ggccttcatg aataccgcct cgtgcaagag cagaattacc ttcatcgatg gtgacaaggg
      841 catactcgaa tatcgtggtt acccgatcga tcaactcgcc gaaaaaagca gttatctcga
      901 ggttgcttat cttttattga acggcgaatt accaaatcag accgaactgg acagttggac
      961 caagaacatc acctaccaca cctttatcaa cgagaacatc aagaaagtga tcgacgggtt
     1021 ccattacaac gcacacccga tgggaatgtt tgaggcgact cttggtgcgt tgtcgacgtt
     1081 ctatcccgat gctaaagata ttttcaatga agagtgtcgc tggaaacaga tctaccgtct
     1141 gattgccaaa gttcctacga ttgccgcttt cgcatttcgc catcgaatag gtatgcagta
     1201 ctcgtatcca gacaatgatc tgagttacga aggaaatttc ctcaacatga tgttcaagac
     1261 gacagagtta aagtaccaac ccaaccctgt gcttgaacga gctttaacgg tcctttatat
     1321 cctccacgcc gatcacgagc agaattgcag tacaaatgcg atgcgcagta ttgggagcgc
     1381 tcatactgat cccttttcag ctttgtctgg cgctgccgcg gccctctatg gaccgcttca
     1441 cggtggtgcg aacgagatgg ttttgcgaat gttgaaggaa atcggctcgg tccagaacgt
     1501 gccggactat atcaagcgag tgaaggcagg cgaattccgt ttaatgggct ttggacatcg
     1561 cgtctacaag aactatgacc ctcgtgcgcg aatcatcaag caggttgctg atgaagtgtt
     1621 tgaggttact ggtaaagatc cattactgga cattgcgctc gagcttgaac gtattgcatt
     1681 ggaagatgat tacttcgtga agcgcaggtt gtatccgaat gtcgacttct attcggggat
     1741 catttaccag gcgatgcgtt tcccggtcga catgtttccc gtgatgtttg cgattccgcg
     1801 cacttcaggc tggttggccc aatgggttga gttgctacag gacgaagatc agaagatagc
     1861 gcgaccgaga caagtctatc tgggaaatcg aactcgcgac tacgtgccta tctcctctcg
     1921 aggctgatca ggagtccagg agtcagaggc cagggtcagg agttattgga gaattatcat
     1981 ttgatatttg agaatttcta tttcgatgca acgagcaccc gtaaacaatt aacaattctc
     2041 aaatatcaat tgataattct ttaatgattc cgcgcgccta ttccatcatt cgcgcagcaa
     2101 tctccttcaa cagcttagcg cacacagtcc cggtgacatg aagagggtcc atgcggggat
     2161 tgaattcgac aatgtctgca cctaccacac tgcctttcaa tcgctgaatt aagtcgattg
     2221 cctggcgcgt agataatccc ccgggctcgc gatgagaaac accgggcgca aacgccggat
     2281 ccagcacatc aacatcaaaa gagatataca agggagagtc aaacgttaat tgtaaaccat
     2341 ctctaaggtc gcgcatctcg atcgtttcaa cattgaactt ttgagcttgt tctcgttgat
     2401 gcgagttgat tgatctgata ccaacctgaa cgagccgctt ggcaagtctg ttctccatga
     2461 ttcgcgcaaa tggactagcg tgagaatact tgttgccctg gtaactatca taaagatcgg
     2521 gatgagcgtc gaagtggaga atgcttaagt ctctgtactt tcgagcgaac gcgcgaacta
     2581 ttggataggt gatcgaatga tcgccgccaa gagagatcgg tttgagtcca tcgtttagaa
     2641 gcgtgaccat tgagttctcg actaacgaca gcatatctgt tccaggaact gcttctacgt
     2701 cgcccgcgtc aaagaatatt gtctcgccgg aaagatcggt tcctgactcg ctcgtcatgt
     2761 tccaggcttc ggaacggagt gcggccctga tttgtggagg agcttctgaa gctcccttca
     2821 tgaatgaaga gttttcgtcg tagcgaaagc caaccaatgc gagattggtg ttttcatgct
     2881 caggagcttt agacatttgt cgtccttaaa aggcaaaaga aaatgaaagg aagattcaga
     2941 ggggccactt atgaaattgg tgcacccggc agggctcgaa cctgcgacct tctgattcga
     3001 agtcagacgc tctatccaac tgagctacga gtgcactgcg gtgaatataa atgctaatcg
     3061 gagaatcgta aagctgcgaa ggattgtttc tgggcagtca tgagggtgcg actagtttcc
     3121 taacattttc catatatcat tttccatttt tcattttggc tgctctgtgg ccagatgaat
     3181 tccattccgg cttaggcttg caactgaatt aaccgtcaaa atgataaatg gaaaatgaaa
     3241 tatggaaaat gctcaggggt gatatctatc gactcgccta ttttccttga aactctttcc
     3301 tgatcaactt cacaagatcg gcaatctcct gcgtctttag cttgtcctta aacgccggca
     3361 tctcttcgtc gccgtccgtt atggtttccg tcaaacgctt atcatcgtgc ttcatcgcgc
     3421 gctcagactt aagtgatgga accttgatct gtttgtcgtt gacctttgct gtcccacctt
     3481 cagccgtagg tccgtgacag ccggcgcact tcttctcata attgattcga gccgatgcca
     3541 actcggaatt gggggccgta ggactagttg cggttggact atgcggggct gggttcgttg
     3601 ccgcagtgtt gttattgcag cccaaaaaag taagcgaaaa agttaacgcg agtaaagcgc
     3661 atccaattcc aattgcgatg ggtttcatcc agtttttctc cgtcgtagag cctcaattta
     3721 aaaagccgtg ggagtataac aataggaaat ccactcggca tccctggcaa gtattacact
     3781 tgcgacagcc gacatcctgt aaagtacatt gcgattaata atgcgattaa taagacgagg
     3841 gccgcggata gtttcttccc tttttcttgc gcggttgcaa tcggcatgga gactgggcag
     3901 aataacctgc atcgcggaag tcattccagg tcagacaatc gtccgaaatc cgaaattttg
     3961 aggtaaatga gatggttagc gacaaggaac aagtcaatcc ccccagaggt tctcttcgcc
     4021 tcgcccctac ttctcaggag gttgaagagc aaaaggctaa gcttcctcga cagtttgtta
     4081 acttcacttt ctatcacgct cgtcccgatt ggcgactgct tcccgatact gagaagcagc
     4141 gctgcaagga ccagtttgtc aaaacagtgg acgagtttcg cagttcgctg ttgatgcact
     4201 gctactcgac gatcggcctg cgaaccaatg tcgacttcat gatctggcga atcggatacg
     4261 atctagagcc attccaggaa atgactgctc gtctgaacaa aactgaaatg gctaaataca
     4321 tcgagccgag tcagtcgttc ctgtctatga cgaagcggtc tatgtacatt gataaggaca
     4381 atcctgaaca tgtagaggat cgcttacaca tcattcccgg caagtctcag tatttgttcg
     4441 tttatccatt tgtcaaaaca cgagagtggt atacgcgtcc cgctgagcag cgacaggaga
     4501 tgatggacga acatattcgg attggctcaa agtatcgttc agttaagctg catacgactt
     4561 attctttcgg gctcgatgat caggaatttg tggtggcatt tgaaactgat catccagctg
     4621 acttcctcga tctggtccag gagttgagag agacgaaggc cagttcgtac accttacgtg
     4681 acactccaat gttcacctgt cgccagcgca cactcgccga gtgcctggac tcgcttgggt
     4741 gatcaagtaa ataacaaagg tccgtaccgt accgcgataa ggttacagta ccgcgagcgc
     4801 agcgaccgga tgaggtactg taaacgtgag tgggtattcg gtcgctgcgc tcgcggtact
     4861 gtaacctcgt cgcgcgctac ggtactgacc tttgctgtga gtgctggaga catcgtccac
     4921 actctcatca ttaaggatta agagttgata ccaacttcaa aaagtttgca gcggtccgtt
     4981 gcccttatcg cgtgtctcat ttttttgacc gccacccatc cagcgatcgt cgccgaccta
     5041 aacatcacac aaacctcaaa gccgcaggaa ggtaactcgg aatatctctg gtatgaggct
     5101 gaaaacatgc ggggtttcac gaccggcaag cttggcgagc caatcgtcaa tccttcttat
     5161 ctggacttac cgaaagagaa gtcacccggg tggggcatga atggtccagg agtctctgct
     5221 gaatggactc aagggggtga aagtgagtgg aactcagccg ccgcaagcgc gaacgaaacg
     5281 aatgcttcga tttatcagga cctcgaagtc ccccgctcgg gaaaatataa gttctgggtg
     5341 cgttacgccg attgggccaa caagaacgaa aacttctcag tcaccatttc tcaaaacggc
     5401 caacgagtgt ttcaacatga gtttggcacg aaagacatta tcgaccctca cgatgaagtg
     5461 agcatgtatt gggggtggtc tttcacctgg gatggtgcag atacgtctct tggcaaaggg
     5521 ccagcgcgag tttcaattga aattcgaaag cctgctgaag cccgaaggaa tgtagattgt
     5581 tttgttctca ctaacgacct tagctatgtg ccatcaaatc gcgagaagcc cgattttgcg
     5641 gcaatgcgct atctgaggca gtggtcatct tcacgagttc ccctggcctc gatcgtacag
     5701 aatgattctg aggcaatacc agatgtttgg ttaagaccta aagtcgctaa tcgcgatttc
     5761 atgatgccct ggaatatcgc cagagagttc tgggctttct atgatcaacc cgaggaacaa
     5821 cgacctattt atcctttcaa cgccgaaacg attgggcaat ttgtcgaacg ttataaaggt
     5881 gtgaaagaag ttccgctctt tgattcaaag ttcgtcgtac cggtgatcta tctcaaccat
     5941 gtacctgaac tctttcaaga aggaagcgcc tttgtgcgct acctca
//
LOCUS       DASSFP010000270         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_375281, whole genome shotgun sequence.
ACCESSION   DASSFP010000270 DASSFP010000000
VERSION     DASSFP010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375281"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFH91_05945"
     CDS             <1..381
                     /locus_tag="VFH91_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862565.1"
                     /translation="SPDLLYKRPPAITFGENILRSAAILEQTFGGLTTNLWDDPFEWT
                     LPETLSSQERIIEYLAEVDATRERAFCCFSDDALTKYVSAPAGEFVLLSVLLDALVRA
                     SDYHGRAAANVKMYSDESTVRFII"
     gene            420..989
                     /locus_tag="VFH91_05950"
     CDS             420..989
                     /locus_tag="VFH91_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862566.1"
                     /translation="MYCPHCGQLQTTGAIRFCSRCGFPLDGVIYLLGNGGMLPIQNSS
                     DEPQPISPRKRGVKQGGILFLSGAVIVPLLAVLGDAAHDSTFLNVLIAFAAIICFVGG
                     PLRMLYAGIFEEGAPNPFRQGARPYVQAPMPAPQQFASHGQTALPPAQSPRPVSSWRS
                     RPVTSELAGPPSVTENTTRLLDKEDRTDG"
     gene            1080..>2038
                     /locus_tag="VFH91_05955"
     CDS             1080..>2038
                     /locus_tag="VFH91_05955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6862567.1"
                     /translation="MESLSDRIRRDGPITFRDWMEAALYDPQFGYYNRSDLNRWGRSG
                     DYRTSPELSDLFAATFASFFAAIYQQLGAPSRWTIVESGAGNGQFAAVVLDRFKRFYP
                     NVYEATNYIIDDVSEDSRRLSRERLATVAEKVEYCELDQLEQVNPGIFFSNELVDAFP
                     VHLLTLEDGAVRELYVELEGDGHFKFSARPVSASKLTQFLERQSVDLKEGQVVEVNLH
                     ISDWLSSIAEKLTAGYVITVDYGAEQDELYGLPERFKGTLRAFGQHQFVDDVLAAPGQ
                     HDITASVNWTQVRTTGERLGLSCISLDRLDQFLLREGLLEELQR"
BASE COUNT          460 a          500 c          541 g          537 t
ORIGIN      
        1 tccccggatt tgttgtacaa gcgtccgcct gccatcacgt ttggcgagaa cattcttcgt
       61 agtgcagcca ttctcgaaca aacatttggc ggccttacca ctaatctttg ggatgacccg
      121 ttcgagtgga ccttaccgga aactctctca agtcaagaac gaattatcga gtatttagct
      181 gaggttgatg ccactcgaga gcgcgctttt tgctgtttta gcgatgacgc gctgacaaag
      241 tatgtttctg ctcctgccgg cgagtttgtg ctgcttagtg ttttactcga tgccctcgtc
      301 cgcgccagcg attaccatgg tcgagccgct gcgaatgtta aaatgtattc cgatgaaagt
      361 acggttcggt ttattatcta acccgtcaaa tgagaattga ttggtgagga tccttctgaa
      421 tgtattgtcc ccattgcggt caacttcaaa ccaccggtgc gattaggttc tgttcgcgct
      481 gtggctttcc cctcgatgga gttatatatc tgctcggaaa tggtgggatg cttcccatcc
      541 agaactcaag cgacgagcct cagcctatct cgcccagaaa gcgtggcgtg aagcagggcg
      601 ggattctgtt tctctcgggc gcggtgatag tgccgttgct cgccgttctg ggggatgccg
      661 ctcatgattc cactttctta aatgtcttga ttgcattcgc ggccatcatc tgttttgttg
      721 gtggtccatt gaggatgttg tacgccggca tttttgaaga aggcgcgccg aacccttttc
      781 gacagggcgc ccgaccctac gttcaggcgc cgatgcccgc gcctcaacaa tttgcctcgc
      841 atggacaaac tgcactgccg cccgcacaaa gtccacgacc tgtctcttcg tggcgctccc
      901 gcccggtgac ttcagagttg gctggaccac cgagtgtcac cgaaaacaca acacgactgc
      961 tggataaaga agacaggacc gacggttagc actttattgt ggtggttcct tgttttcttg
     1021 atgatgtaga agggaagcgt gggttggcca cggagggcca cacctacaac agaagaccct
     1081 tggagagtct tagcgatcga atcagacgag acggtccgat cacttttcgt gattggatgg
     1141 aagcagccct ctatgatccg cagttcgggt actacaatcg ctcagatttg aatcgttggg
     1201 gccgttcggg cgactatcgc acaagccccg agctgagcga cctatttgcc gcgacatttg
     1261 ccagtttttt cgcagcgata tatcaacaac tcggcgcgcc ttcgcgctgg acgattgtcg
     1321 aatccggagc agggaacgga caatttgccg cggtcgttct ggatagattc aaaaggttct
     1381 atccaaatgt ctacgaagcg acgaattaca ttatcgacga tgtaagtgag gattcccgtc
     1441 ggttgtcacg agagagattg gcaaccgttg ctgaaaaagt cgagtattgc gagttagatc
     1501 agcttgaaca ggtaaaccct ggaatcttct tttcgaacga actggttgac gcgtttccgg
     1561 tacatctttt gacacttgaa gacggcgcag tgcgtgaact ttatgtcgaa ttagagggag
     1621 atggccattt taaatttagc gcccgcccgg tttctgcatc aaagcttaca cagtttttag
     1681 aacgacagtc cgttgaccta aaagaaggtc aagttgtaga agtgaattta cacatttctg
     1741 attggctatc ttcaattgct gagaagctga ccgccgggta tgtaatcacc gttgactatg
     1801 gcgctgagca ggacgaacta tacggtttgc cggaacgttt caaaggaact ctgcgtgctt
     1861 tcggtcagca ccagtttgtc gacgacgttc ttgcggctcc tggccaacac gacatcactg
     1921 cgagcgtgaa ctggactcaa gtgaggacaa caggtgagcg actcggattg agttgcataa
     1981 gtcttgacag gctagatcaa tttctattgc gcgaaggttt gcttgaagaa ttgcagcg
//
LOCUS       DASSFP010000271         8762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_970353, whole genome shotgun sequence.
ACCESSION   DASSFP010000271 DASSFP010000000
VERSION     DASSFP010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970353"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="VFH91_05960"
     CDS             complement(<1..1649)
                     /locus_tag="VFH91_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6862568.1"
                     /translation="MKWLLSLFILALTPVLASAQVWTAKLDKDVQFYQTTDFGIVVVG
                     TEKSLYAIDGATGEILWRRKDIAVDENDVAPIPGTDLVLLSLEKSNKSRIEAVDLLSG
                     SSIWTSEKLSGAIMQTAVDFQQKLLAVVLVKDAKNRARDNYKQRPILQVLNLGSGRVL
                     WKYEMGEVEMMPTKWPEDKGKEVHYCLDNYYPPAFVDGRLFTFYEGVTSFESLDGKER
                     LREKYRVNEEGLALTEAQPIFTSGSIYVSGRGHVRAISRDNGDTQWEAKDLGLTPEMI
                     LVNDVLVVRTGGQFTRLENGETVQRGPYGISAVEARTGKVLWRYKSADKGITNLVIAS
                     PNTIAAADNDEVFYLDFQTGKRLRRQSHKIEGAAYALLNESSQIVIGGKSEIAAFDLS
                     GNEAWRVQRNPPGRGWLRTVSAIAARAASLYFRFGGTTTTVFSGLRIANTAMRFSWSG
                     LSLGSVSNLQALATSASRGYSARRFDNFGIASKLGGSNSRSVGGLSRNRVSDMEDGLM
                     DRLDPAKQLERLSRYLWHRDRLATLRGNWMYFYTDLKTMDG"
     gene            2175..3230
                     /locus_tag="VFH91_05965"
     CDS             2175..3230
                     /locus_tag="VFH91_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862569.1"
                     /translation="MFSRFICVVLLSFGADLVKAQSLDPTVPAPVRSNQLTGRIAPRD
                     LGDSRLTDHYFAFTGTPGDLVITVQSTNLNGDIDVFTAGTLRPLLKLTLYAETSAAVT
                     KGIYLRKRENLILRVEARSPNDDEGTYRLSFSGSFEPIIGGPDIAEAETPSTTPGFTR
                     DGRRTKRVSSVGARINEPEPPAEVASSSATPEARPEPSPEATPVETPSVTETEKPASP
                     KTPKPPAPRNSRGRRPAPRKTPAPKPAETAQVEEPPKPEGETESTATPVKPKSTPRTS
                     SRGTETKRQPKPAPEPEPEIGPRLIIETNYGMLINRSMSTVKRVTVENGQVVVTGKDG
                     TVDRIMLVNVLRMSIAP"
     gene            complement(3324..3920)
                     /locus_tag="VFH91_05970"
     CDS             complement(3324..3920)
                     /locus_tag="VFH91_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862570.1"
                     /translation="MSRENLLFAIIGILFGFIVGFIFASTMAQKNATAPTSTSSQAMP
                     ADHPPVGGPNSGGDPQAVRAEVAASLEKAKSEPNNFEAQLKAAELYYQIQRYDQAIEF
                     LLKANQLQPTDYRTVVILGMVNLDAGHFETAEKWYRAALKMKKDDVMVLAGLSEATLQ
                     KGDAKAAADAIANLEKVDPNSQDLPRFRDKLASLKSGK"
     gene            complement(3930..4814)
                     /gene="ccmI"
                     /locus_tag="VFH91_05975"
     CDS             complement(3930..4814)
                     /gene="ccmI"
                     /locus_tag="VFH91_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HET6862571.1"
                     /translation="MILFWVVCTVLLVIALAFIVPTALQRSPANLERAEDELKQANIT
                     IYRDQLSELKADLENGIVSQEQFAIDRDEIERRLLEDADQPRSKKQSVLTAPSARTAY
                     LLAIGIPCLAVGLYLKIGEPKSITGQVNPRPSGPMSSMAEGGGRSEEQIEANVAALAK
                     RLQTNPSDLAGWTMLARSYNSMQRYGEAAGAYAKATDLSPKDADLWAEYAMASAMANG
                     RELQGKPTELINQALKVDPKNAKALQLAGTAAFQSKNYKKAVEYWEQVLSQVPADSEV
                     AQAVKDRISEAKTQGNLK"
     gene            complement(4811..5287)
                     /locus_tag="VFH91_05980"
     CDS             complement(4811..5287)
                     /locus_tag="VFH91_05980"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET6862572.1"
                     /translation="MPRFIGLVFLVLLAFGPVAANDAQPLASDPALEKRLNNLSKELR
                     CLVCQNETLADSQADLAEDLRREIREQMQAGKSDQEIINFLTQRYGQFILYRPQVTPT
                     TYLLWFGPFVLLLAGLVVLFKYIKQRRDLIVEQPLTVAQRRMAEELLSSDSGKDSA"
     gene            complement(5288..5821)
                     /locus_tag="VFH91_05985"
     CDS             complement(5288..5821)
                     /locus_tag="VFH91_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET6862573.1"
                     /translation="MLRYLLPLGVFIVLVVFLAIGLGRDPHEVPSPLINKAAPTFRLP
                     QLKEPTKTFSAEEMRGKVWILNVWASWCISCREEHPLLIQYAKSGAVPIYGLNYKDKR
                     DDALAWLSELGDPYVLSAADEDGRVAIDYGVYGAPETYLIDKNGTIRFKQVGAVTPDV
                     WQQKILPLAQDLNRGTK"
     gene            complement(5824..7839)
                     /locus_tag="VFH91_05990"
     CDS             complement(5824..7839)
                     /locus_tag="VFH91_05990"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET6862574.1"
                     /translation="MIPEVGQFALIIALLLAIVQSSLPLIGASRGNRTWIALAAPAGQ
                     AQFIFVAIAFGCLAYSFITNDFSVLNVATNSNSQLPLHYRLAATWGSHEGSLLLWTLM
                     LALWTVAVSLFSKHLPEEMVARVLSVMGIVSMGFLLFMLLTSNPFARLFPVPPDGRDL
                     NPLLQDPAMVAHPPMLYMGYVGFSVAFAFAISALIGGRLDATWARWSRPWTTVAWMFL
                     TCGIALGSFWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGGFKSWTV
                     LLAIAAFSLSLLGTFLVRSGVLTSVHAFATDPKRGIFILAFLVIVIGGSLLLYAWRAK
                     QVGIGGKFDLISRESLLLSNNVLLTVAAGSVLLGTLYPLIVDALGMGKISVGPPYFNA
                     VFVPLMAPAVFLMGVGPIARWKKATLPDLVTRLRWAFVISVVSAVALPFVFGQWKPLV
                     SLGLLLAIWIVATVFANLWERVRVTTGQVTTLQKLRAQTRSYYGMQVAHLGVAVFIIG
                     VTIVTGYQLEQDVRMEVGDTVNAGGYEFKFNGVSDVTGPNYRAARAEIVINRNGAEVE
                     RMYPEKRNYTASGNVMTETAIDSGLFRDLYISLGEPVAGGAWSVRVYYKPFVGWIWGG
                     AVLMAIGGGLALSDRRYALAARKQRQVPEAKAEKVPALAAASVEAES"
     gene            complement(7866..8324)
                     /gene="ccmE"
                     /locus_tag="VFH91_05995"
     CDS             complement(7866..8324)
                     /gene="ccmE"
                     /locus_tag="VFH91_05995"
                     /inference="COORDINATES: protein motif:HMM:NF009731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET6862575.1"
                     /translation="MKRRHKRIAFILVGLAGLSAAAFLVASAFRNNLVFFFSPTQVAA
                     KEAPVNRTFRVGGLVEQGTVKRDSDGLTVRFTVTDTAASIPVVYKGILPDLFKEGRGC
                     VAQGKIGEDGVFHADQVLAKHDENYMPPEAGQAIDKAKHAQEAAKTVVDN"
     gene            complement(8308..8493)
                     /gene="ccmD"
                     /locus_tag="VFH91_06000"
     CDS             complement(8308..8493)
                     /gene="ccmD"
                     /locus_tag="VFH91_06000"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HET6862576.1"
                     /translation="MSLSEFLSMGGYGFYVWGSYLVSLIAITAELISLSRRKKALAPQ
                     AKPKPVIRGSITNETTS"
     gene            complement(8493..>8762)
                     /locus_tag="VFH91_06005"
     CDS             complement(8493..>8762)
                     /locus_tag="VFH91_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862577.1"
                     /translation="ADRAGAVLAIVGVINVPIIYFSVQWWNTLHQGATIRILGKPSIA
                     APMLAALLIMLAACWLYSVAVVLIRLRCIILEREQNAARLAEVAV"
BASE COUNT         2204 a         2343 c         2148 g         2067 t
ORIGIN      
        1 ttcccatcca tagtcttcaa atctgtataa aagtacatcc agtttccgcg caacgtcgcc
       61 agacggtcgc ggtgccatag atagcgcgaa agtctttcca actgtttcgc aggatccagt
      121 cggtccatca agccatcctc catgtcgcta acccggtttc gactaagacc tccgacactc
      181 cttgagttgg agccgcccag tttcgatgca atcccaaagt tgtcgaagcg cctggcgcta
      241 tagccgcgac tggctgaagt tgcgagcgct tgcaaattcg aaactgagcc aagtgaaaga
      301 cccgaccagc tgaaccgcat tgcggtattt gcgatacgaa gtccgctaaa aactgtggtg
      361 gttgttcccc caaaacggaa atagagagac gctgctcggg cggcgattgc gctcactgtg
      421 cgcagccacc ctcggccagg tggatttcgc tgaacacgcc atgcttcatt gccggataag
      481 tcgaacgcgg caatctctga tttcccgcca attacaattt gcgaagactc attgagaagg
      541 gcatatgcgg ccccttcgat cttatggctt tgacggcgca aacgttttcc tgtttggaaa
      601 tccagataaa aaacttcgtc attgtcggca gcggcgatgg tgttcggact agctatcacc
      661 aaattcgtta tgcctttgtc cgccgactta tagcgccaca acacttttcc cgttcgcgcc
      721 tcgacagcac taatgccgta gggaccacgc tggacggttt cgccattttc caaacgagtg
      781 aattgcccgc ctgtgcgaac aaccaggaca tcgtttacga ggatcatttc tggagtcaga
      841 ccgagatctt ttgcttccca ctgtgtatcg ccgttatcac gtgaaatcgc gcggacgtgg
      901 ccacgaccag aaacgtagat ggagccggaa gtgaagattg gctgagcctc agttaaagca
      961 aggccttctt cgttaactct atatttctct cgcagtcgct ccttgccatc gagactctca
     1021 aatgaagtta ccccttcgta gaacgtgaac aagcggccgt caacaaaagc tggtggatag
     1081 taattatcga gacaatagtg aacctctttt cccttatcct cgggccattt agttggcatc
     1141 atctcaactt cacccatctc atacttccaa agcacccggc cggatccaag attgagtacc
     1201 tgcaaaatcg gccgttgctt atagttgtcg cgcgctcgat ttttcgcatc tttcacgagg
     1261 acaactgcga gaagcttctg ttggaaatcg acggcggttt gcatgattgc accgctcaat
     1321 ttttcgctgg tccaaatgga gctgccgctt aacagatcaa cagcttcgat tcggctcttg
     1381 ttactttttt cgagactgag taggacgaga tcagtgccag gaatgggcgc gacatcattt
     1441 tcatcgaccg ctatatcttt ccgacgccag aggatctcgc cggtcgcgcc atcgatcgca
     1501 taaagtgatt tttcagtgcc gacaaccacg attccgaagt cagtcgtctg ataaaactgg
     1561 acatccttgt cgagtttagc tgtccagacc tgggccgacg cgaggaccgg tgttagagct
     1621 agaataaata gggataacaa ccacttcata acgggagtat agcaatttat atgactctgg
     1681 aattatttgc gaatcttaat atctctatgc acctcactac ctcggcccta aatagatttt
     1741 gttcaccgca gagtcgcagc gcagcagagc cgcaaccaat gaaggatgtg gctggaacag
     1801 gccagtagcg attcgcggtg tccgatatgc ttcagcttgt cggtttgact cgttcctctg
     1861 aacttgagtc ttcatcatga ccgacaagcc gaagcatatc ggacatctcc ggaatcggta
     1921 ctgacctgtt cccgcagtac tctgaatggc aaggcatact gtccgaagaa actgtgtcgg
     1981 aaaaacaaga tcttaaaaac ttgctacgca gagaacgcaa gggaacgcga tctgaaccaa
     2041 tacaagttct ttcgacttta agacaccttc tgataaatct ggtattctta caggaagatt
     2101 tttgttgatg attctgcgtt ctgcgcgaat tcctccgggt tcagcaaatg aaatttcgtc
     2161 ttaaatcgag ttctgtgttt tctcgtttta tctgcgttgt tttgctctcc tttggcgcgg
     2221 accttgtaaa agcacagtcg ctagacccta ctgttcctgc tccagttaga agtaatcaac
     2281 tgaccggaag aatcgctcca agagatctgg gcgactctcg acttaccgat cattactttg
     2341 cgtttaccgg gacacctggc gatcttgtca taacagttca gagtacaaac ttgaatgggg
     2401 atatcgatgt ctttactgct ggcactttaa gaccgctttt gaagttgacg ctttacgctg
     2461 agacctcagc cgcagttacc aaagggatct atctgcgcaa gcgcgaaaat ttaatactgc
     2521 gtgttgaggc tcgtagcccg aatgacgacg agggaaccta cagactttct tttagcggct
     2581 cgttcgaacc aataattggt ggtccagata tcgccgaagc tgagacaccg tcaacgacgc
     2641 ccgggtttac tcgtgatggc cgccggacaa aaagagtttc ttcagttgga gcgcgcatca
     2701 atgagcctga accaccggcg gaagtcgcca gctcgtcggc aacgcctgaa gcaaggcctg
     2761 agccgtcgcc tgaagccacg cccgttgaaa cgccgtcagt aaccgagact gaaaaaccgg
     2821 cgtcaccgaa gactccgaaa cctcctgcac ctcgcaactc caggggacgt cggcccgcgc
     2881 ctcggaagac tcccgcgcct aagccggccg agacagcgca ggttgaggag ccaccgaagc
     2941 cggaggggga aaccgaatcg accgcaactc cggttaagcc taaatcaact ccacgaactt
     3001 cttcgagagg aaccgaaact aagcgtcaac ccaagccggc gcccgaaccg gaacctgaaa
     3061 ttggaccacg attgatcatc gaaactaact atggaatgtt gatcaatcga tcgatgagta
     3121 ctgtcaaaag ggtaactgtc gagaatggtc aggtggttgt gaccggcaag gatggcacgg
     3181 tcgatcgaat catgctcgtg aacgtgctac gcatgtcgat cgcgccctaa ttttggagtg
     3241 cgacggcccg gcgccgcttt tttatatagc cttagaattg gaagggatct gggtaataaa
     3301 gcggcgccgg ccgcactcca aagttatttc cctgacttta agctggcgag tttatcgcgg
     3361 aagcgaggta gatcttgact gttgggatct actttctcga ggttagcgat cgcgtccgct
     3421 gcagctttag cgtcaccttt ttgcaaggtc gcttcgctga gtcctgcaag aaccataaca
     3481 tcgtctttct tcatcttcag cgccgctcgg taccacttct ctgcagtttc aaagtgacct
     3541 gcgtctaagt tcaccatacc cagaataact acagttcggt aatcagttgg ttgcaactga
     3601 ttcgctttga gcaggaattc aattgcctga tcgtagcgct gaatctggta gtaaagctct
     3661 gctgccttca attgagcttc gaagttattt ggttcgctct tggccttttc cagtgaagcg
     3721 gcgacctcag cgcgtactgc ctgcggatca ccccctgagt ttggaccgcc aactggagga
     3781 tggtcggcag gcatagcttg actgcttgtg ctggttggcg cagtcgcatt cttttgggcc
     3841 atcgtactgg caaaaataaa gcccacgatg aagccgaaca gtattccaat gatcgcaaac
     3901 agtaaatttt ctctcgacat gagttgcgtt tattttaggt tcccctgggt tttagcttcg
     3961 ctgatacggt ccttgactgc ttgagcaact tcggaatctg cgggaacctg gctgagaact
     4021 tgttcccagt actcgacggc cttcttgtag ttcttagatt ggaatgccgc agttcctgca
     4081 agctgtagag ccttcgcatt cttcggatcg accttcaatg cctgattaat aagttcggtc
     4141 ggcttaccct gtagttcacg accattcgcc attgcggagg ccatcgcgta ttcggcccaa
     4201 aggtcagcgt ctttcggcga caggtccgtg gctttggcat aagctccggc ggcttcgcca
     4261 taacgttgca ttgagttgta tgagcgggcc aacatggtcc aacccgcgag atcggatgga
     4321 ttggtctgca gacgtttcgc caacgccgca acattggcct caatctgttc ttccgagcgc
     4381 ccaccaccct ccgccatact cgacatcgga cctgacggcc gcggattaac ctggccagtg
     4441 atacttttgg gttcgccaat cttgagatag agccctacag ccaaacacgg tatgccaatc
     4501 gcgagaagat aagcggtgcg ggcacttggt gctgttaaga ccgattgttt tttactgcgg
     4561 ggctgatctg catcctccaa taagcgtctc tcgatctcat cccgatcaat agcaaactgt
     4621 tcctgactta cgatgccgtt ctcaagatcg gctttaagtt cacttaattg atctcgatag
     4681 atagtgatgt tcgcttgttt aagttcatct tcagctcgct ccagattggc tggcgagcgc
     4741 tgtaacgctg ttggcacgat gaacgccagc gcaataacta agagcacagt acaaacaacc
     4801 cagaagagta tcatgccgaa tcctttcctg aatcagaact caacaactct tcagccattc
     4861 ttcgttgggc aacggtcaag ggttgctcca caatcagatc gcgtcgttgc ttgatgtact
     4921 taaaaagaac caccaggcca gcgagcagca agacgaaagg accaaaccac agcaggtaag
     4981 tggttggtgt aacctgcggg cggtagagaa taaattgacc gtatcgctgc gtaagaaagt
     5041 taataatctc ttgatcgctt ttgccggcct gcatctgttc gcgaatttct cgtcgtaaat
     5101 cctcagccag atcggcttgc gagtcagcca gagtttcgtt ctgacaaacc agacaccgaa
     5161 gctctttcga gaggttgttc aggcgctttt cgagtgcggg atcacttgcg agaggctgcg
     5221 cgtcgttagc tgcgaccgga ccaaaagcta aaagaaccag aaaaactaag ccgataaatc
     5281 gtggcatcta tttagttccc ctgtttaggt cctgagcgag aggtagtatt ttttgttgcc
     5341 atacatcagg tgtgacagcg ccaacctgtt tgaagcgaat ggttccattc ttatcgatca
     5401 aataagtttc gggagcacca tacactccat aatcaatcgc gactctccca tcctcgtcag
     5461 cggcacttaa cacatatggg tcaccgagtt cggagagcca cgccaatgca tcatctcgtt
     5521 tatctttgta gttcaatccg taaataggca ccgcgccaga tttcgcatat tggatcaaga
     5581 gtggatgttc ctcgcggcat gatatacacc acgaagccca aacattaaga atccaaacct
     5641 tacctcgcat ttcttcagcg gagaatgttt tggttggctc ttttaactgc ggcagtcgaa
     5701 aggtcggagc cgctttatta atcaatggcg aaggaacctc gtggggatcg cggcccaggc
     5761 caattgccag gaaaacaacc aggactataa agacacccag cggtaataaa taacgaagca
     5821 taattaagac tcggcctcga ctgaggcagc agcaagcgcc ggaacttttt cagccttcgc
     5881 ttccggcacc tggcgttgtt tgcgagcggc cagcgcgtac cggcgatcgc ttaatgcgag
     5941 accgccgccg attgccatta aaactgcgcc gccccagatc caacctacaa atggtttgta
     6001 gtagacacgc acactccagg ctccgcccgc aacgggctcg ccgagagaga tatacaaatc
     6061 acggaaaaga ccactatcaa tagcagtctc agtcatgacg ttgccgctgg cggtgtagtt
     6121 acgcttctct ggatacattc gctcgacttc ggcgccgtta cgattgatga cgatttcagc
     6181 acgagccgca cgatagtttg gaccggtaac gtcactgaca ccattgaact tgaactcata
     6241 tccgccggcg ttcacagtat cgccgacttc cattcgcaca tcttgctcta attgataacc
     6301 ggtaactatc gtcactccaa taatgaagac cgcaactccc aggtgtgcga cctgcatacc
     6361 gtagtagctg cgcgtttggg cccttaattt ctgcaaggtc gttacctgcc cagttgttac
     6421 tcgcactcgt tcccacaggt tcgcaaagac agttgcgaca atccagatcg ccagcagcag
     6481 cccaagactt acaagaggtt tccattgccc gaagacaaac ggcaacgcaa cggcgctgac
     6541 gacactgata acaaacgccc aacgcagtcg agtaacaagg tcagggagcg tggccttctt
     6601 ccaacgagct attggaccaa ctcccatcag gaacactgcc ggcgccatca ggggtacaaa
     6661 taccgcattg aaataaggag ggccgacgga gattttgccc atcccaagtg catcgacaat
     6721 caagggatag agggtaccga gcaataccga gccggcagct acagtgagta gtacattgtt
     6781 ggagagcagg agtgattctc tcgagataag atcgaacttg ccaccgattc caacttgctt
     6841 tgctcgccag gcatacagca gtagcgatcc accgataacg atcaccaaaa acgcgagtat
     6901 aaaaatgccc cgcttgggat cggtagcgaa tgcgtgaacg gacgtaagaa ctcccgaccg
     6961 aacgagaaat gttccgagta agctcaagga aaacgccgca attgcgagaa gaacggtcca
     7021 gcttttaaaa ccgccgcgct tttcagtgac ggcgagagag tgaatcaaag cagtaccaac
     7081 cagccacggc atgaatgaag cgttctcgac cggatcccaa aaccaccaac ctccccagcc
     7141 aagttcgtaa taggcccaga aactaccgag cgcaattccg caggtcaaaa acatccaggc
     7201 gacagtggtc cagggtcgcg accacctggc ccatgtggca tctaaacgcc caccaattag
     7261 cgcagagatc gcgaaagcaa aggcgactga aaacccgacg taacccatgt aaagcattgg
     7321 tgggtgagcg accatcgccg gatcttgcaa gagtggattg agatcgcggc catccggggg
     7381 caccgggaag agacgagcga atgggtttga agtcagcaac atgaacaaca aaaaacccat
     7441 gctgacaatt cccattacac tcaggactct cgcgaccatc tcttctggca agtgtttact
     7501 gaacagcgac actgcgacag tccagagtgc cagcatcaac gtccagagaa gcaatgatcc
     7561 ttcatgcgag ccccacgtcg cggccaaccg atagtgaagc ggtagctgtg agtttgaatt
     7621 agtggcgacg ttcaataccg aaaagtcatt ggtgataaat gagtaggcca aacatccgaa
     7681 cgcaattgcc acaaagatga actgggcttg tcccgctggt gcagccaggg caatccatgt
     7741 tcgattaccg cgagacgctc cgatcagggg aagactcgac tgaacaatcg cgagcagcag
     7801 cgcgatgatt aaagcaaact gaccaacttc aggaatcatt attttggcta tgcagttatt
     7861 agctgctaat tatcaactac ggttttagct gcttcctgag catgttttgc cttatcaatt
     7921 gcctgacctg cttccggtgg catgtagttc tcgtcgtgct tggctaaaac ctggtcggcg
     7981 tggaacactc cgtcttctcc gatctttccc tgggcaacac agccgcgtcc ttccttaaat
     8041 agatcgggga gtattccctt ataaaccact ggtatgctcg ctgcggtatc ggtgacggtg
     8101 aaacgtacgg tcaatccatc gctatctcgt ttgacggttc cttgctcgac aagaccgcca
     8161 acgcgaaacg ttctgttgac aggagcttcc ttcgccgcaa cttgagtggg actaaaaaag
     8221 aagacaaggt tgtttcggaa tgcgctggca accaggaatg ccgcggcgct caggccagcc
     8281 agtccaacta aaatgaatgc aatgcgctta tgacgtcgtt tcattcgtga tactacctcg
     8341 aattacaggt ttgggtttcg cttgcggcgc caatgccttc ttccggcgac tcaagcttat
     8401 caactctgcg gtgatagcaa taagcgacac gaggtacgag ccccaaacat agaatccata
     8461 gccgcccatt gagagaaatt ctgaaagact cattagactg ccacctccgc gagtcgagcc
     8521 gcattttgtt ctctttcaag aataatgcag cgaagccgga ttagtacaac tgcaaccgaa
     8581 taaagccagc aagcagcaag cattattagt aaagcagcta acatcggcgc cgcaatactt
     8641 ggcttgccca ggattctaat ggtggctccc tggtgtagtg tattccacca ctgcaccgaa
     8701 aagtaaatga tcggcacgtt aatgaccccg acaattgcca gtacggcccc ggcgcggtca
     8761 gc
//
LOCUS       DASSFP010000272         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_222304, whole genome shotgun sequence.
ACCESSION   DASSFP010000272 DASSFP010000000
VERSION     DASSFP010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222304"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VFH91_06010"
     CDS             complement(<1..1471)
                     /locus_tag="VFH91_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET6862578.1"
                     /translation="MMKKLYCFVTLVLLVLTISVSAQEKRLSERVAETAMNSLWHDAA
                     KNHSAAPPKWTYEYGVVLEGLEGVWLNTGDGRYFQFIQKGIDNFVENDGNIRTYNSGD
                     YNLDNVLSGRSLLLLYKVTGQDKYRKAAALLRNQLNTQPRTSDGGFWHKKIYPSQMWL
                     DGLYMAEPFYAEYAATFNESNDFEDIAKQFVLMENHSRDVKTGLLYHGWDESKQQRWA
                     DPVTGHSPNFWGRAMGWYAMALVDTLDYFPRQHPKRAELLAILNRLASAVLKYQDPQS
                     GLWYQVIDKGKEKGNYLEASGSSMFVYALAKGYRNGYLPGSSFIAAERGYKGLINRYI
                     KSTANGETSLEGTVSVAGLGGNPYRDGSYDYYLSEKVVTNDPKGIAAFLMASNEFDIA
                     ANRTVGFGKRVTLDSYFNNEIKKDANGQSVSWHYKWDELPNSGFSLWGHVFRNYGVKT
                     DMLMDAPTIANLKNSAIYIIVDPDTKEETPTPNFIEPKDV"
     gene            complement(1504..2943)
                     /locus_tag="VFH91_06015"
     CDS             complement(1504..2943)
                     /locus_tag="VFH91_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HET6862579.1"
                     /translation="MGNNPLSRRQFLRNAVVGGTALAVLPQILFHESIDAQIANPWTE
                     VMPQILQRIKPPTFPQRDFVITRFGAKPDGQAYATNAFKAAIDACNKAGGGRVIVPAG
                     TFLTGPIHLKSNVNLVISRGATVKFSQNPNDYLPAVFSRWEGVELLNYSPFVYARDQQ
                     NIAITGEGTLDGQSDAEHWWPWNGRAAYGWKEGTPNQRADRTRLMEMAESNVLVEQRL
                     FGAGHYLRPQFIQPYHCQNVLIEGVSIVNSPMWELHPVLCTNVTIRNVRINSHGPNND
                     GCDPESCSDVLIEGCDFNTGDDCIAIKSGRNADGRRLNMPTQNIIVRRCRMRDGHGGI
                     TVGSEISGGVQNLFAEDCNLDSPNLDHALRVKNNAMRGGLLQDLHFRNIQVGQVAHAV
                     ITIDFNYEEGAKGNFVPVVRNYTVDKLTSSKSVHALDIQGLTGAPIYDLRLQDCTFEN
                     VAEPNVVRNVRNATLENVRINGKEITQIP"
BASE COUNT          816 a          843 c          757 g          898 t
ORIGIN      
        1 tcacatcctt tggttcgatg aagtttggtg ttggtgtctc ttctttggtg tccggatcta
       61 cgatgatgta gatcgctgaa ttcttcaagt tcgcgatagt tggcgcatcc atcaacatgt
      121 cagtcttgac gccgtagttc ctgaaaacat gtccccataa cgagaagccg ctgtttggta
      181 actcatccca tttgtaatgc catgagacgc tttgtccatt agcatctttt ttgatctcgt
      241 tgttgaaata cgaatcgagt gtgacgcgct tgccaaaacc aaccgtacga ttggcagcaa
      301 tatcaaactc attgctggcc atcagaaacg cagcaatccc tttcggatcg ttcgtgacta
      361 ccttctcgct taagtagtag tcgtagctac catcacgata cggattgcca cccagtccgg
      421 cgacacttac ggtcccctca aggctggtct cgccattggc ggtacttttg atgtaacggt
      481 tgataagacc cttgtaacca cgctcggccg cgatgaaaga acttccgggg aggtaaccat
      541 tacgataacc tttggccagc gcgtacacga acatagagga gccggacgcc tcaagataat
      601 ttcctttttc tttcccttta tcgatgactt gataccacaa acctgactgc ggatcttgat
      661 acttaagtac tgctgaagcg agtcgattga gtatcgcaag taattccgca cgtttagggt
      721 gttgtcgcgg gaaataatct aaagtgtcga ccagcgccat cgcgtaccaa cccattgctc
      781 gtccccaaaa attgggtgag tggcccgtta ccggatcggc ccagcgctgt tgtttcgact
      841 cgtcccaacc gtgataaagc aaacctgtct ttacatcgcg cgaatgattc tccatcaaga
      901 caaactgttt agcgatatcc tcgaaatcgt tggactcatt gaacgtggcc gcgtactcag
      961 cgtaaaacgg ctctgccatg tacagaccat cgagccacat ttgactggga taaattttct
     1021 tgtgccaaaa cccgccgtct gatgtacgtg gctgagtatt gagttgattg cgcagtaatg
     1081 ccgctgcttt ccggtactta tcctgacctg tgaccttgta aagaagtaac aaacttctgc
     1141 cggacagcac gttgtccagg ttgtaatcgc cagagttata agtgcggatg ttgccgtcat
     1201 tctcgacaaa gttgtcgata cctttctgga tgaactggaa gtagcgacca tcgcccgtgt
     1261 tcagccaaac accttcaagc ccttccagca cgactccata ttcatacgtc cacttcgggg
     1321 gcgcggctga gtggttcttt gcagcgtcat gccacaaaga attcatcgca gtttctgcga
     1381 ctcgctcgga gagtcgtttc tcttgagctg agacagaaat cgtcagcacc aggaggacta
     1441 acgttacgaa gcaatataat tttttcatca taggcactat gcttgacacc cctcacacat
     1501 ttgctacgga atctgagtta tctctttccc attgatcctg acattctcca gtgttgcgtt
     1561 cctcacattc cgcacaacat tgggttcggc aacgttctca aaagtgcaat cctgtaatcg
     1621 cagatcgtaa atcggggcgc cggttaaccc ttgaatgtcg agcgcgtgaa cactcttcga
     1681 acttgtcagc ttgtcgactg tatagtttcg gactactgga acgaaattgc ccttagctcc
     1741 ttcctcataa ttgaaatcga tagtgattac cgcatgcgca acttgtccaa cctggatatt
     1801 tcggaaatgt aaatcctgaa gcagaccgcc acgcatcgcg ttgttcttca cccgtagtgc
     1861 atggtccaaa ttgggactgt ctaaattaca atcctccgca aaaagatttt gcactccgcc
     1921 tgaaatttca ctgccaacag ttatacctcc atggccatcc ctcattcggc agcggcggac
     1981 aatgatgttt tgagtcggca tgttgagcct tctaccatca gcattccttc cggatttgat
     2041 cgcgatgcaa tcgtcacctg tattgaaatc acaaccctct atcagaacgt cagaacacga
     2101 ttctggatca catccatcat tgttcggacc atgactgttg atgcgaacgt ttctgattgt
     2161 aacgttcgta caaagtacag gatgcaactc ccacattggt gagttgacga tgctgacacc
     2221 ttcaatcaga acgttctgac aatgataggg ttgaataaac tgtggtctaa gataatggcc
     2281 ggccccgaac agtctttgct caacaaggac atttgactcg gccatttcca tcaagcgagt
     2341 tcgatcggca cgttgatttg gagttccctc tttccaacca tacgcggccc taccgttcca
     2401 tggccaccag tgctctgcat cactctgtcc gtcaagtgtg ccttcaccgg tgattgcgat
     2461 gttctgctga tctcgtgcat agacgaatgg cgaatagttc agcagctcca ccccctccca
     2521 acgagagaat actgcgggca ggtagtcgtt gggattctga ctaaatttaa cggttgcacc
     2581 tcgagagatc accagattga cgttgctctt caggtgaatt ggaccagtaa ggaaagtgcc
     2641 ggccggaaca atcacccgac cgccgcctgc tttgttacaa gcgtcaattg ctgctttgaa
     2701 agcgttcgtg gcataggctt gtccgtctgg cttcgctcca aatctggtaa tgacaaaatc
     2761 tctctgagga aaagttggag gcttaatgcg ctgcaggatt tgcggcatca cctcagtcca
     2821 tggattcgcg atttgagcat caatcgactc atggaagaga atttgaggta gaacagccag
     2881 agccgttcca cctacaacgg catttctcag gaattgacga cgagagagtg gattatttcc
     2941 cattggtcat taaccattta ccatcttcat ttgttaatgc gccttgccga aatttgcccg
     3001 taccgtcacg ctggtcttac catccaccca ctaacgtttt tccggggaaa tggtatcagc
     3061 caataatgtt gaatgctgga atgaacgaaa tccttgttaa aactgggtag ttaggcgggc
     3121 gcgattctgc cttacagcta aacgactgtc aaggtttacc atctttacca attgaggatt
     3181 cttggcggtg cggtaagacc agaagcaggg ttaggggcag ggtgggacgg aatcatttga
     3241 gaattatgaa ttgagaattg agaatttcta tttcgatctg gatgactccg ttcaacacca
     3301 gtaaccaaaa taac
//
LOCUS       DASSFP010000273         5376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1266281, whole genome shotgun sequence.
ACCESSION   DASSFP010000273 DASSFP010000000
VERSION     DASSFP010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266281"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1628
                     /locus_tag="VFH91_06020"
     CDS             360..1628
                     /locus_tag="VFH91_06020"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6862580.1"
                     /translation="MAPPELASSKTKPAVVHKRSAFAALRHRDFRLLWLGQIVSVTGS
                     QMQSVAINWHVYVLTKSAFALGLVGLFRGLPIILCSLIGGVVADAVDRKRLMIITQVM
                     MLVSAGLLTAFTIVGIESVWPIYLLSCFASAAIAFDIPARQSLMPTLVPPEDFPNAVS
                     LGLIVFNVATICGPAIAGFLLAESGPAIIYAFNAVSFLAVIAALLMMRVSGRPEFDGS
                     TPSALNVGALKEGLNFVWRTPIIVQTMTLDFAATFFGSATLLLPLFAQERLHVDARGY
                     GFLAAAPAVGSVLTALVMARIGTFRKQGRAVVISVAVFGLATAAFGASRIYWVSLLML
                     ALTGASDTVSTVLRQTIRQLVTPNYLRGRMTSINMMFFMGGPQLGELEAGSLAHVTGA
                     PVSVIVGGLGAIVCACVAAMRSRSLMNYDS"
     gene            1642..2199
                     /locus_tag="VFH91_06025"
     CDS             1642..2199
                     /locus_tag="VFH91_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862581.1"
                     /translation="MNWTHLETSSHQDRVIAHVIGATLLGHFILDETAFLLLDIGFVW
                     NIYLDGEMGLLPHPVAISELTITDESKNAIQTDVDRLLAGYTSGELQLVKSFGVGVPI
                     TEVSLFQNSDGRRINIDCERGSVIIETSIHKGEVKIMANDESELAETVREEAEFLKDK
                     LREQLGREPTDEEVNEWLREHTESY"
     gene            complement(2265..2852)
                     /locus_tag="VFH91_06030"
     CDS             complement(2265..2852)
                     /locus_tag="VFH91_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6862582.1"
                     /translation="MAYDEQEAKRSRVVVETPNERREVSHSQSVRTPEGNGISAGMVG
                     VLVVVAIALITMLVLFYMNSQKQPTDQSVATQQQQPPPPTVIQQPAQQPPVIIQQPAP
                     ATQPAPIVVTPPAGGTATESAPNIDSTVQAAIDKAFTDDPTFKDLGITATVLDGKVTL
                     LGTVKTEDLKNQAERMVRKIKGVKSIDNQILVVAE"
     gene            complement(2943..3158)
                     /locus_tag="VFH91_06035"
     CDS             complement(2943..3158)
                     /locus_tag="VFH91_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862583.1"
                     /translation="MPVENDTDDDKHLDSGGTEGMRRVLVFLVVLGLLVVTGTLISAD
                     AQSRQVREIGFEKPHIVEMNTTTLNEL"
     gene            3396..4331
                     /locus_tag="VFH91_06040"
     CDS             3396..4331
                     /locus_tag="VFH91_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET6862584.1"
                     /translation="MTTAIVHHPVFREHDTGPGHPETASRYTVVMEALRSSSGLNLVE
                     VEAHGAPRGDIQAAHSPQLYKTVERVVSEGTAYLDADTVVSMRSLDAATHAAGAPCQA
                     IDLVMNGQVKNAFVPVRPPGHHATSERSMGFCLFNNVAVAAKYAQNHYAEIERVAIVD
                     WDVHHGNGTQGIFYDDPSVFFFSMHQYPWYPGTGTRGEKGTGRGLGYTSNLPLRAATP
                     ANEQRRGFEAALEDINSTFVPDLILISAGFDSHIDDPLGQLLLADTDFTAMTRAVKQW
                     ANDACKGRLVSCLEGGYNLDTLGQTALAHVTELQR"
     gene            4356..5171
                     /locus_tag="VFH91_06045"
     CDS             4356..5171
                     /locus_tag="VFH91_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6862585.1"
                     /translation="MHLYRTRQGPVVESNQRYFSLSVEWDELINQSDLLGYLNAHIAP
                     ENESKLEDVLAPIGSQEVWAAGVTYYRSRTARMEESQQAGGGNFYDRVYNAERPELFF
                     KSSAHRVAGPGESVRIRKDSKWNVPEPELTLAINSKGDIIGYTIGNDMSSRDIEGENP
                     LYLPQAKVYDRSCALGPCILVNSEPLAVSTEIRMRLERKESEVFHGSTTLAQLKRSPS
                     ELVEFLFRDNSFPLGCFLLTGTGIVPPDDFTLQSGDRIEIAIDGIGTLSNVVA"
     gene            complement(5184..>5376)
                     /locus_tag="VFH91_06050"
     CDS             complement(5184..>5376)
                     /locus_tag="VFH91_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862586.1"
                     /translation="ATAEGGPNLLHLEEGNWKVMDLAKVPEDPKNPLGAEDASAGFVR
                     NLMKTFPGVPRDIFPKPSH"
BASE COUNT         1216 a         1359 c         1400 g         1401 t
ORIGIN      
        1 gggtgagtct cgagatacac aagcttgcag gtacttggag actcacccga cgcgagtcgg
       61 tactgacctt tgtcaactgc caattcaatc actcactact gctatccgaa gccttgacct
      121 caaacccgtt gagcgtctga ttctctctgc gtctctctgc gtgtctctgc gatgctaacg
      181 agaaccaatt ttggctgagt cggaggagcg cagagacacg cagagatatc gcagagagac
      241 gcagagagaa gactttctgg aatcatttag ttggaaaggc ggaaagtagt ttcgagcctg
      301 gcccccgacc cctgaccctg cctttgtctt gttgcgcttg accttgtaat ttattaccga
      361 tggccccgcc tgaacttgca agctccaaaa ctaagcctgc cgtcgttcac aagcgatcag
      421 cgtttgctgc tttgcggcat cgtgacttcc gtctgctttg gctcggacaa attgtttcag
      481 tcaccggatc gcaaatgcag tcggttgcaa tcaactggca tgtttatgtg ctcacgaagt
      541 ctgcgtttgc ccttggcctg gttggtctgt ttcgtggctt gccgataatc ctgtgctcgt
      601 tgatcggagg cgttgtcgct gacgccgtag accgaaaacg cctgatgatt atcactcagg
      661 tgatgatgtt ggtaagcgcg ggactcctca cggcattcac gattgttggg atcgaaagtg
      721 tctggcccat ttacttgctc agctgttttg cttctgccgc aatcgccttt gatattccgg
      781 cgcgacaatc attgatgcca acgctggtgc cgccggagga ttttccaaat gcggtcagtt
      841 tgggactgat agttttcaat gtcgccacca tttgcggacc tgcgatagcg ggcttcctgt
      901 tggctgagag tggtccagcc atcatttatg cattcaatgc cgtctcattt ttagcagtga
      961 ttgcagcgct actaatgatg agggtgagtg ggcgcccgga gttcgacggc agtacaccca
     1021 gcgcactcaa cgtcggtgcc ttgaaagaag ggctaaattt cgtttggcgc accccaatta
     1081 ttgtacagac catgacacta gacttcgcgg cgaccttttt cggttctgca acattactct
     1141 tacccctatt tgcgcaggag cgtctacatg tcgacgcgcg cggatacggg tttctggctg
     1201 ccgctccagc cgttggctcg gtccttacag cgttggtcat ggcgcggatc ggaacttttc
     1261 gcaagcaagg ccgagctgtc gtaatttccg tcgctgtttt tggtctcgcc actgccgcgt
     1321 ttggggcctc caggatttac tgggtatctc tgctgatgtt ggcgttgacc ggcgcatcag
     1381 atactgtcag cactgttctc agacaaacga ttcgtcaact tgttacgccg aactatcttc
     1441 gtggtcgaat gacctcgatc aacatgatgt tctttatggg agggccacaa cttggcgagc
     1501 ttgaggcggg ctcgctcgct catgttaccg gagctcccgt atcggttatc gtcggcggtc
     1561 ttggagcaat tgtgtgtgcg tgtgtggccg ccatgaggtc caggagtttg atgaactacg
     1621 attcctgagt ctccgctaaa aatgaattgg acgcaccttg aaaccagtag tcatcaagat
     1681 cgtgtaatcg cgcacgtgat tggcgcgacg ctactgggcc acttcatttt ggatgaaaca
     1741 gcattcttgc ttctcgacat aggctttgtg tggaatatat acctcgatgg agagatgggg
     1801 ttactacctc atccagtggc catttcggaa ttaactatta cagatgagtc taagaacgcc
     1861 attcaaaccg atgtcgatcg acttctcgcc ggttacactt ccggtgaatt acaactcgtt
     1921 aaaagtttcg gggttggcgt tccgattacg gaagtcagtc tcttccaaaa ctcggatggg
     1981 cgacgaatta acattgattg cgaaagagga agcgtgataa tcgagacctc gattcataag
     2041 ggagaggtga aaataatggc caatgatgag tcggaactcg ccgagactgt cagggaagaa
     2101 gcagaattct tgaaagataa gctacgtgaa caacttggtc gcgagcccac tgacgaagaa
     2161 gtcaatgagt ggctccgcga acacactgaa agctactgat ttccagaaag tacaaagccg
     2221 cgtctccgat cgaaacgcgg cttgatgtta cctgccgggg tgagttattc agctacaacc
     2281 agaatttggt tatcaattga cttgacgcct ttaatctttc gaaccatgcg ttcagcctga
     2341 ttctttagat cctctgtttt cactgttccc agtagcgtta cttttccgtc taacacagtc
     2401 gcagtaatac cgagatcctt gaacgtcgga tcatctgtga acgctttatc gattgccgct
     2461 tgaacggttg agtcgatatt cggcgcactc tcagttgctg tgcctccggc cggcggtgtt
     2521 acaacaattg gcgctggttg tgtagcaggt gccggttgtt gaatgatcac cggtggctgc
     2581 tgcgccggtt gctgaataac agtaggcggt ggtggctgtt gttgctgggt cgccacgctt
     2641 tgatctgttg gctgtttctg actattcatg tagaagagta caagcatcgt aatgagcgca
     2701 atcgctacga cgaccagtac tccaaccata cccgccgata ttccatttcc ttccggtgta
     2761 cgcacagatt gtgaatggga aacttcgcgc cgttcgtttg gtgtctctac aactacacgg
     2821 ctacgctttg cctcttgttc gtcgtatgcc atcgtctttt gtctccgttc ttagttgaaa
     2881 ggtcctgttt ttaaaacgtg atgccgatcc ttctaatcga attcgggagt caggcgaaga
     2941 actcagagct cattcaaagt cgtcgtgttc atctcaacaa tgtgtggttt ttcaaagccg
     3001 atttctctga cttgacgact ctgagcatca gctgaaatca atgtcccggt tacaaccaat
     3061 aagcccaaca caaccaggaa aaccagtact cttctcatgc cctctgttcc ccccgaatct
     3121 aaatgtttgt cgtcgtcggt atcattctca acaggcactt cgcaaaggtc gttccgcaag
     3181 gttctacgcg agcatgagcc caattctcaa gggaaatcat ttccacgaca ttagttcagg
     3241 ttgtttttgg agcaccaagc ctgtctgaat atggtacgcc tccgaaactc gcatcaacac
     3301 tcggtcagtt tcgttttaag acacttcgaa gatcgcagtg aacgaagttg agatcgcagc
     3361 ttcactaaat ccagttcaac atttaggatt catccatgac cacggcaata gttcatcatc
     3421 cagtcttccg cgagcacgac accgggccag gacaccccga aaccgcgtca cgctatacag
     3481 tagtcatgga agcgctcaga agttcgtcag gcctcaatct cgtcgaggtg gaagctcatg
     3541 gtgccccgcg gggtgacatc caggccgcac attcgccgca gctttacaag acggtcgaac
     3601 gggtcgtgag tgaaggtacg gcctacctcg atgcggacac ggtcgtctca atgaggtcgc
     3661 tcgacgccgc aactcatgcg gcaggcgcgc cctgccaggc catagacctg gtgatgaatg
     3721 gccaagtaaa aaacgcgttc gttccagttc gtccgccagg acatcatgcg acttcagaac
     3781 gctctatggg attttgcctg ttcaacaatg tggccgtggc ggcgaagtat gctcagaacc
     3841 actacgcaga gattgaacgg gtggcgatcg tcgattggga cgttcatcac ggaaacggca
     3901 cacaaggtat tttctacgac gatccaagcg ttttcttctt ctcgatgcat cagtacccgt
     3961 ggtatccggg aacgggcacg cgaggagaga aggggactgg tcgcggtttg ggttacacga
     4021 gcaatcttcc actgcgtgcc gcaacgccgg ccaatgagca gcggcgaggt ttcgaagcag
     4081 cgctcgaaga tatcaattcg accttcgtgc cggatctgat ccttatttca gcaggatttg
     4141 attctcacat tgacgatcct ttaggccaac tgttgttagc agatacagat ttcaccgcga
     4201 tgactcgagc cgtcaaacaa tgggccaacg acgcttgcaa aggcagattg gtttcatgtc
     4261 tggaaggtgg gtacaacctg gatacgcttg gtcagacggc tttagctcac gtcaccgagt
     4321 tacaacgtta gagtctattt gaggactttt tcttcatgca tctttaccgc accaggcaag
     4381 gtccggtcgt tgaaagcaat caacgctact ttagtctcag cgtcgaatgg gatgaactga
     4441 tcaatcagag cgacttgctg ggatatctaa atgcacacat tgcacccgag aatgagtcga
     4501 agcttgagga cgtccttgct ccgattggat cgcaggaggt ttgggctgca ggtgttactt
     4561 actatcgaag tagaaccgca aggatggaag agtcgcaaca ggctggaggc gggaactttt
     4621 acgatcgcgt ctacaatgcc gagcgtcccg agctgttctt caagtcgtcg gcgcatcgcg
     4681 tcgcgggtcc tggagaaagc gtccgcattc gtaaagactc aaagtggaat gtacccgagc
     4741 ctgagctaac actggcgatt aatagtaagg gcgacataat tggttacacg atcggcaatg
     4801 acatgagttc acgcgatatt gaaggcgaga accccctcta cttgcctcaa gcaaaagtgt
     4861 atgatcgcag ttgcgctctt ggaccttgca ttttggtcaa tagcgaacct ctcgcggtct
     4921 cgacagagat tcggatgagg ctggagcgga aagaatctga agtgtttcac ggctctacaa
     4981 ctctggccca gctgaagcgt tcaccctcag aactggtcga gtttcttttc cgggataatt
     5041 catttccgct gggttgtttc ctgctgactg gaacaggaat agtgccccct gacgatttta
     5101 cgctgcaatc gggggatcgc attgagatcg cgatcgatgg gattggaacg ctgagtaatg
     5161 tggtggctta gccgatcctg atcttaatgc gatggcttcg gaaaaatatc gcgtggcact
     5221 cctggaaagg tcttcatcaa gttcctgacg aaaccagcac tggcgtcttc tgcgcctaga
     5281 ggattcttag ggtcttccgg cacctttgct agatccatta ctttccagtt tccctcttct
     5341 aaatgcagta gatttggccc accttcagca gtagct
//
LOCUS       DASSFP010000274         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_377052, whole genome shotgun sequence.
ACCESSION   DASSFP010000274 DASSFP010000000
VERSION     DASSFP010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377052"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFH91_06055"
     CDS             <1..681
                     /locus_tag="VFH91_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET6862587.1"
                     /translation="SLAVAAKLNGNQFARRNKFWQLASSRGTPKEIYAISRQLFSANQ
                     IASISKLDPGPELTNCNGRNGLDVINTISRLELQGYMANTLLRDTDCMSMAHSLEVRV
                     PFVDKHVVRNILSLPGEWKLDSRNKVPKPLLAEILHDLLPADFLSRPKMGFTLPFEKW
                     MLSKLRNDISRVLADDKLMESACLSSKGVTRLFEGFLAAPRSIGWSRPWSTYALARWC
                     EINRVARS"
     gene            692..1858
                     /locus_tag="VFH91_06060"
     CDS             692..1858
                     /locus_tag="VFH91_06060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6862588.1"
                     /translation="MKVLHVIPSISKRSGGPAHAILPMCKALQRAGLDVTLATTDHDL
                     ENADSRQGDLSTLDGVPVHIFAAQLGDSFKYSRPFSSWLNSEVENFDLVHIHAVFNHS
                     SIAAARACTRKNVPYIVRPLGTLDPWSMKQKPIRKRLFLKLGARRLLTNAGAIQYTTE
                     AEKSSTEAALRLNHGIVVPLGIEEELSGVEISKEQFGKMFPTLQDRPYILVLSRLHSQ
                     KNIDALLRAFITVSARPNFSQWQLVIGGDGNSDYVAQLKRLVGANDRVLFTGWLEAVA
                     KYAILRNASLLALTSFQESFGLCLLEAMACGVPVLVGSNVKLAADIDKARAGWITEPQ
                     LEAITQALSTALENPTELKTRGMSGVAFARSFTWESVGISLSSLYSSVVNQSRL"
     gene            1858..2634
                     /locus_tag="VFH91_06065"
     CDS             1858..2634
                     /locus_tag="VFH91_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6862589.1"
                     /translation="MLKQITPLILTYNEGPNIKQTLDRLTWADDIVVVDSFSNDDTLE
                     IVGQYKQARVLKRQFSSISDQWNFGLTEGRINTEWVLSLDADYVLTDEFVEELKNLRP
                     NSAIDGYQARFIYCIKGQRLRSGIYPPVTVLFRKTKSSYTSDGHTQRVIINGAVGALE
                     SAILHDDRKPLDRWFEAQRRYSELEAEKLLTTDSRLLNWKDRLRKWRVVAPPAMLFYC
                     LIIRGGILDGWAGFYYAFQRTFAELILSLRLIEGSDKTNK"
     gene            2639..4465
                     /locus_tag="VFH91_06070"
     CDS             2639..4465
                     /locus_tag="VFH91_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET6862590.1"
                     /translation="MIILGINAYHGDAAAALIRDGRLIAAAEEERFNRIKHSAGFPSA
                     AIRWCLQEAGIGIEDVDHIGISRDPSAHLHKKVLFAATRAAKQVSPGRTRGTDTIETL
                     DVDSFSDTVKSNGRGSGVLQQVRDRLQNAARVRDLKDGIARLFEVSKSRLRAHFHNIE
                     HHRAHLASAFYVSPFDQAALLSIDGFGDFISTMWAKGEGNSIEILGQVEYPHSTGIVY
                     TATTQFLGFPHYGDEGKVMGLAPYGQPRFINEFREIIRTDEGGRFRLNLNFFRHHAEG
                     VEMTWDQGSPVIGQIFTGEFARTFGPARCSGAEITDRERDIAASLQLRLEEVCFHILN
                     HLHDLTGSTDLGLAGGVAYNSVMNGKILLNTPFKRIFVQPAAGDSGTAIGVCYEIFNR
                     LLRNQRSEVMQGAYTGPEFGPDRIRAALESAKVRFETYSESETTKRAAQDIADGLVVG
                     WFQGKMEFGPRALGNRSIVVDPRRAEMKEILNDRIKKREPFRPFAPSILEEFAGDYFE
                     QSHPAPTMLMVYQVREEKRSLIPAVTHVDGSGRLQTVSRVVNDRYYQLISDFNELTGI
                     PVILNTSFNENEPIVCSPEEAISCFLKTRMDVLYLGNYAVRR"
     gene            4484..>4799
                     /locus_tag="VFH91_06075"
     CDS             4484..>4799
                     /locus_tag="VFH91_06075"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET6862591.1"
                     /translation="MRILVTGGAGFIGSHLCERLLDEGNEVLCLDNFFTGQRENIHQL
                     LDNPRFELLRHDVIQPILLEVDQIYNLACPASPIHYQYNPVKTVKTSVMGMINMLGLA
                     KRV"
BASE COUNT         1210 a         1181 c         1227 g         1181 t
ORIGIN      
        1 tcgctggccg tcgctgcgaa actgaacgga aatcaattcg cccgtcggaa taagttttgg
       61 caactcgcat ccagtcgcgg aacgccaaag gaaatttatg caatctcgcg acaacttttt
      121 tcagcgaacc agatcgcctc gatttcaaaa ctagatcctg gaccagagct tacgaactgc
      181 aacggccgga acggactcga tgtcatcaat acaatatctc gtcttgaact tcagggatat
      241 atggcgaaca ccctgttgcg tgacactgat tgcatgagca tggctcactc gctggaggtt
      301 cgagtaccgt ttgttgataa gcatgtggtc aggaacatct tgtcgttgcc cggcgagtgg
      361 aagttagaca gcaggaacaa agtaccgaag ccgcttctcg ccgaaatact gcacgatctg
      421 ctgcccgccg attttttatc acgaccaaag atgggcttca ctctgccttt cgaaaaatgg
      481 atgttgtcaa aactccgcaa cgacatttcg cgagtgctgg cagatgacaa gctaatggag
      541 tcagcgtgtt tgagcagtaa gggtgttacg cgtcttttcg aaggtttcct ggcagcgcca
      601 cgttccatag gatggagtcg gccatggtcc acctacgctc tggccaggtg gtgtgagatt
      661 aatcgggtcg cgcgctcttg agtacaaatt tgtgaaagtt ctacacgtga tcccttcaat
      721 ctcgaagcgc tcgggtggcc cagcccatgc aattcttccg atgtgcaaag cgctgcaacg
      781 cgcaggtctc gatgtcacgc ttgcgactac cgatcacgac cttgaaaacg ctgattcgcg
      841 acagggcgat ctatcaacgt tggatggagt gcccgtccat atcttcgcag cgcaattagg
      901 cgattcattc aaatactcgc gccccttctc cagttggcta aattcagagg tcgaaaattt
      961 tgaccttgtc cacattcatg ctgtctttaa tcattcgtcg attgctgccg ctcgcgcgtg
     1021 taccaggaaa aatgtccctt acatcgttag accgcttggc actctggatc cctggagcat
     1081 gaagcaaaag cccattcgaa agcggctgtt tctaaaactc ggcgccaggc gcttattaac
     1141 taatgcgggt gccatccagt acacaaccga ggcagaaaag agctccactg aagcggcgct
     1201 aaggctaaat catggaatag tagtgcctct gggaattgag gaagagcttt cgggtgttga
     1261 gatctctaaa gagcagtttg ggaagatgtt cccgacgctg caagatcgtc cttacattct
     1321 ggttttgtct cgactacact cgcaaaagaa tatcgacgct ttgttgcggg cattcataac
     1381 agtttcagcc aggccaaact tttcgcagtg gcaacttgtg attggcggtg atggcaactc
     1441 ggattacgtt gctcaattaa agagactagt aggtgcaaac gatcgagtgt tatttaccgg
     1501 ttggctcgaa gctgtggcga aatacgcgat tctacgaaac gcatcgttgc ttgccttgac
     1561 ctccttccag gagagttttg ggctgtgttt acttgaggcg atggcttgcg gcgtgccagt
     1621 tctggtaggt tcaaacgtca agctggccgc agatatagac aaggccagag ctggatggat
     1681 cacggagcca caactggagg cgataactca ggcgctgtca acggccctcg agaacccgac
     1741 cgaattgaaa acgcgcggaa tgtcaggagt tgcattcgcg cgcagcttca catgggagag
     1801 tgtggggatc tcgctgtcat cactttactc ttcagtggtt aatcagagtc gcttgtgatg
     1861 ctcaagcaga taacgccact catccttaca tataatgagg gtccaaacat aaaacaaact
     1921 ctcgatcgct taacctgggc cgatgatatt gtcgtggtgg acagttttag taatgacgat
     1981 acgttggaaa tcgtgggcca atacaagcag gcccgcgtgt tgaagcgaca attttccagt
     2041 atctctgatc aatggaactt cggattaaca gaaggtcgga taaacacaga gtgggtactg
     2101 agccttgatg cagattatgt acttacggac gagtttgtcg aagaactgaa aaaccttcgg
     2161 cctaactcgg caatcgacgg ataccaggcg cgttttatct actgcattaa aggtcaaaga
     2221 ttacgaagtg gaatataccc accagtcacc gtcctcttca gaaaaaccaa aagcagctat
     2281 acatccgacg gtcacacgca acgagtgata attaacggag cagtcggcgc acttgaatca
     2341 gcaatactgc acgacgatcg aaaaccactc gatcgctggt tcgaagctca aagacgttac
     2401 agcgagcttg aagcggagaa gttgctcacc acagattcca gactgctgaa ttggaaggat
     2461 cgtcttcgta agtggcgagt agtcgcgccg cccgccatgc tcttctactg cttgataatt
     2521 cgcggtggaa tactcgacgg ctgggcaggc ttttattatg cgtttcaacg gacctttgcg
     2581 gaattaatcc tgtctctacg acttatcgaa ggctccgaca aaaccaacaa atagatttat
     2641 gatcattttg ggaatcaatg catatcacgg cgatgccgcg gcagcactga ttcgtgatgg
     2701 ccggttgatc gcagctgccg aagaggagcg attcaatcgg ataaagcact ccgcaggttt
     2761 tccatcagca gcaattcgct ggtgccttca ggaggctgga atcggtattg aagacgtgga
     2821 ccacatcggg atatctcgag atccttcggc ccatcttcat aagaaagttc tgtttgccgc
     2881 tactcgagct gccaaacaag tttcgcctgg ccgaacgcga ggcactgaca cgatcgaaac
     2941 actcgacgtt gattccttct cagacactgt taagagtaat gggcggggct caggtgttct
     3001 gcaacaagtg cgtgaccggc tacaaaacgc cgcgcgagtg cgagacttga aggatggcat
     3061 tgctcgatta ttcgaagtct ccaaatcgag gctacgagct cactttcaca acattgagca
     3121 tcatcgagcc catctcgcga gtgctttcta cgtttcacca tttgatcagg ccgcccttct
     3181 ttccatcgat ggctttggag actttatctc aacgatgtgg gccaagggtg aagggaactc
     3241 aattgaaatt ctcggccagg tcgaatatcc gcattcaact ggaattgtct acacggcgac
     3301 gacacaattc cttggctttc cacactacgg cgacgaaggt aaagtgatgg gtcttgctcc
     3361 atatgggcaa ccccgtttca taaacgagtt tcgcgaaatt atccgcaccg acgaaggagg
     3421 cagattccgt ttgaacttaa acttcttccg ccaccatgct gaaggtgttg agatgacatg
     3481 ggaccagggg tcgccagtca tcggacagat cttcaccggt gaattcgccc gcacctttgg
     3541 cccagcccgg tgttccggag ccgaaataac tgatcgcgaa cgggacattg cagcgagtct
     3601 gcaactgcga cttgaagaag tttgctttca cattttgaat cacctccacg acttgactgg
     3661 ctcaactgat ctcggattgg caggcggagt cgcatacaac tcggtgatga acgggaagat
     3721 cttgcttaat acgcctttca aacgaatctt tgtacaaccg gctgccggcg acagtggaac
     3781 agcaattggt gtttgctacg aaatctttaa tcgcctgttg agaaaccagc gaagcgaagt
     3841 aatgcagggc gcctacactg gtccagagtt tggccctgat cgaattcggg cagcacttga
     3901 aagtgccaaa gttcgatttg aaacctattc cgagtcagaa acaaccaaac gcgcggcgca
     3961 agacatcgcg gatggtctgg tcgttggttg gtttcagggc aagatggagt ttgggccacg
     4021 cgccctcggt aatcgctcaa ttgttgtaga cccgagacgt gctgagatga aagagatcct
     4081 taacgatcgc atcaagaaac gcgagccgtt tcggcctttc gctccgtcca ttctcgaaga
     4141 attcgcgggt gactatttcg agcagtctca tccagccccc accatgttga tggtctatca
     4201 ggttcgcgag gagaagcgat cgctgattcc ggcagttact catgtggatg gctcgggcag
     4261 gctgcagacg gtatcgaggg tggtgaacga tcgttactat cagctgatct ccgactttaa
     4321 cgaactcact ggcataccag tcatcctaaa cacttcgttt aatgaaaatg aaccaatcgt
     4381 ttgtagtcca gaagaagcaa tttcctgctt tcttaagact cgaatggatg ttttatacct
     4441 ggggaactac gctgttcgca gataaattac tggagaatat caaatgcgca ttttagtaac
     4501 gggtggtgca ggttttatcg gttctcatct ttgtgagcga ctgcttgatg aaggcaatga
     4561 ggttctttgt ctcgataatt tctttaccgg tcagcgagag aacattcacc aactgctcga
     4621 caatccgagg ttcgaattgc tgcgccatga cgtaattcaa ccgatactgc tcgaggtgga
     4681 ccagatttat aatctcgcct gtcctgcctc tccaatccac tatcagtaca atccggtgaa
     4741 gactgttaag acaagcgtga tgggcatgat taatatgctc gggctggcca agcgagttc
//
LOCUS       DASSFP010000275         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1703233, whole genome shotgun sequence.
ACCESSION   DASSFP010000275 DASSFP010000000
VERSION     DASSFP010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703233"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFH91_06080"
     CDS             <1..773
                     /locus_tag="VFH91_06080"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6862592.1"
                     /translation="ILVIFSAAITAHAQTSYQFDNFDFANGVQVQTPPPAKAMSANPQ
                     DGKKLIKPTLKYMPAPGALTATVPLNPSLNGFTTGSADVDGYIVESGKRNQVDPLLLY
                     SIMHQESSFKTRAMSYKGARGLMQLMPPTAVRFGVSNIWDPKQNIEGGARYMRFLLDL
                     FSGDVSLALAGYNAGEGAVMKFGFRVPPYSETQEYVRRIGRRYSLIRDPDAAAHASKL
                     SPDQVATARGKNATPLNVYEPTVFMARLPDGRLQLVTQ"
     gene            900..1481
                     /locus_tag="VFH91_06085"
     CDS             900..1481
                     /locus_tag="VFH91_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6862593.1"
                     /translation="MSCSNCGAYMAPSVRFCSNCGAPAADPESTRIARLQEMDLQRGI
                     DPELEHVVFTVRPTLIFIKAGYALAVLGGVALVFLLAMISVPAYISIPLALALLLIPA
                     YYHIRRNSVRYTATDAKLEIDRGLVSRTTRNIPLSKIQDVTVSATIPQRLLGLGDLVV
                     DNASEVGGSTVLHNINNPRQHADLLLRELRRWH"
     gene            1552..2706
                     /locus_tag="VFH91_06090"
     CDS             1552..2706
                     /locus_tag="VFH91_06090"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HET6862594.1"
                     /translation="MNSTFSRFILAFVVVIASVTVICAQVPDELKQLLSPKVDKFSQV
                     SSAKLIDKTLDPSAFGGVPPVATEYRSPAGEKFVVSVFRSRSDTDSYSGLTHAARKAE
                     ETTQRPTQLNREIGTSSFRTTSQISFFKGTSLVHVSTKSGNDFARVEDGIALARALAD
                     RIDKGEGDIPPLVKHLPDPENSQNSAVYFSKVDAPGSILPGQAVLTALETNGDADAVI
                     ARIGQSKVLIVEFNTPQLATDNDQRIVAKIHELWDQGQPAPTAYRRVGNYAVMVFDAP
                     DQKTANQLIDQVKYEQVVQWLGENPNILKEAQRRYVDTTLGVFIAVVKASGFALIGCL
                     GAGGLIGALLFSRRRAQQRQVEAFSDAGGMLRLNLDELTPKSRGLLRDRN"
BASE COUNT          761 a          826 c          818 g          800 t
ORIGIN      
        1 ttattctggt tatattttcg gccgcaataa ctgcgcacgc ccaaacttcc taccagttcg
       61 acaactttga ctttgccaat ggggtgcaag ttcagacacc tccaccagct aaagccatgt
      121 ccgcgaaccc gcaagacggg aaaaagctga tcaagccgac gctgaagtac atgcccgctc
      181 cgggtgcatt aacggctacc gttccgctaa acccctctct gaatggcttc acaaccggga
      241 gcgctgacgt cgacggttac atcgttgaat cgggtaaacg taatcaggtc gatccgcttt
      301 tgctctattc gatcatgcac caggagtcga gtttcaaaac gcgcgctatg tcctacaagg
      361 gggctcgcgg gctgatgcag ttgatgccac ctacagccgt gagattcggc gtttcgaata
      421 tctgggatcc taagcagaac atcgagggtg gtgcccgtta tatgcgtttc ttgcttgacc
      481 ttttttcggg cgatgtatcg ctagccctag cgggatataa cgccggcgag ggcgctgtta
      541 tgaagttcgg ctttcgcgtt ccgccctata gcgaaactca agaatacgta cgccgaattg
      601 gtcgtcgcta ctcactcata cgggatccag atgctgcggc tcacgcaagt aaactttcgc
      661 cggatcaggt tgcaaccgct cgcggaaaaa atgccacgcc gttgaatgtt tatgaaccaa
      721 ccgttttcat ggcgcgtttg cccgatggtc gtctccagct ggtgactcag taggcctggc
      781 ctttcaaaca agttcgaggc ggaacccgca agggtctccg cctcgttttg tatttatcgg
      841 gctttgagta tgatggtttt actctcctat ggttttctgg cacgcgaggg ccaatctcta
      901 tgagttgttc gaattgcgga gcatacatgg ctccatccgt gaggttttgt tctaactgtg
      961 gtgctccggc agcggatccg gagtcaaccc ggattgcacg gctccaggag atggatcttc
     1021 agcgcggtat agatccggaa ctggaacatg ttgtgttcac cgttcgaccg actctgattt
     1081 tcatcaaggc gggttatgct ctggcagttc ttggcggggt agccctcgtc tttttgcttg
     1141 cgatgataag cgttccggcg tacatctcta tccccctggc actggcgctg cttttaattc
     1201 ctgcgtacta ccacattcga cgaaattcag ttcgttacac agctacggat gcaaagctgg
     1261 agatcgatcg aggattagtt tcacgcacca ccagaaatat tcctttgagt aagatccagg
     1321 acgtgacagt ttcggccacg ataccccagc gattgttggg gttgggtgac cttgtcgtgg
     1381 acaacgcgag tgaggtgggc ggctcgacgg ttttgcacaa catcaacaat ccccgacagc
     1441 acgctgactt gcttttgcgg gaactcagac gctggcatta aactccattg agtcggaact
     1501 aaaacagata ggggaacatc taagtgaatt ccgggttcta agtaagctct tatgaattca
     1561 acgttttcaa gatttatctt agcttttgtg gtcgtaatcg cgtctgtaac ggtaatttgt
     1621 gcgcaagttc ctgatgaact caagcagtta ctttcgccga aggttgataa gttttcacaa
     1681 gtcagctcag caaagctgat agacaagaca cttgacccga gcgccttcgg aggcgttcca
     1741 ccagtggcga cagagtatcg ttcacccgca ggcgagaagt tcgtagtaag tgtttttaga
     1801 tctcgcagcg acaccgattc gtattccgga ctgacgcacg ctgccaggaa ggctgaggag
     1861 acgactcagc gcccgactca gcttaatcgt gaaattggca cctcgagttt tcgtacaacc
     1921 agtcaaatat cgttcttcaa aggaacatcg ctcgtccatg tatcaacgaa atcgggaaac
     1981 gactttgccc gagtcgaaga cggaatagcg ttggcgcgcg cattggcaga tcgaatcgat
     2041 aaaggtgagg gagacattcc acctctggtt aagcatctgc cggatcccga gaacagtcag
     2101 aactcagcgg tctacttttc caaagtcgat gcgcccggct caatacttcc cggacaggca
     2161 gttttgactg cgttagaaac aaacggtgac gcagatgccg taattgctcg cattggtcaa
     2221 agcaaagtct tgattgtcga gttcaacaca cctcaactcg ccaccgataa cgaccaacgg
     2281 attgtcgcga aaatccacga gctgtgggat cagggtcaac ccgcaccaac tgcttatcga
     2341 cgagttggca attacgcggt gatggtcttc gatgctcctg atcagaagac agccaatcag
     2401 ttgatcgatc aggtcaaata cgagcaggtg gtccaatggt tgggcgagaa cccgaatatt
     2461 cttaaagaag cccaacggcg ctatgttgac acaactctgg gagtctttat tgcggtagtg
     2521 aaagcttccg ggtttgcgtt gataggatgc ctcggtgcag gaggtctaat tggagctcta
     2581 ttgtttagcc gcaggcgtgc gcaacaacga caggtggaag ctttttcgga cgccggtgga
     2641 atgctccgac tgaatctgga cgagttgacg cctaagagcc gagggttgtt gagggatagg
     2701 aattaatagg accaatacga cctataggac ctattcttta atccaaaaac aaaaaagcgc
     2761 ggcgacagat gtcgatcgcg cttcttcatt taaaccggtt caaattgcgg gctgctgatt
     2821 tttcctggga ggctccccca agccccatca agacgacaac cagcagcacc gcgatttacg
     2881 ctcatcgact gcgtcacaag cgccactctg ttcccccaaa gagtcaaaac gcttgctgta
     2941 aatcctgtga gaggatgcct cccccaaagc ctccactcac cgcaattgtc tgagcctgac
     3001 ccgatttacc ctcatctacc cttcttgttc tgatttgtct attggcaacg gacatgccag
     3061 aagggatcga aatttaactt tcaccgattt tgtgggactc ttttgcaaat tcaggcgaga
     3121 tcgcagtcta gctttctctt cgccttgcgt acaattttcg tacgaatcgg cagtacaacc
     3181 gaccgataat cgctcggatc gcttg
//
LOCUS       DASSFP010000276         7409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1562861, whole genome shotgun sequence.
ACCESSION   DASSFP010000276 DASSFP010000000
VERSION     DASSFP010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562861"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..642
                     /gene="atpH"
                     /locus_tag="VFH91_06095"
     CDS             103..642
                     /gene="atpH"
                     /locus_tag="VFH91_06095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET6862595.1"
                     /translation="MSLQTVARRYASALADVIIERREERQVQDEVLQWASIIEDHVQL
                     REVFTNPTVAYDQKRQLLEELITRTRVRETTASFLRVLLKNQRLGELKEISVKLGQVL
                     DDRSGVVAAVVTTARPLPENLKSNLEESLTASAGRRVRLSFATDESIIGGLVARIGST
                     IFDGSVQSQLERLAADLTD"
     gene            676..2262
                     /gene="atpA"
                     /locus_tag="VFH91_06100"
     CDS             676..2262
                     /gene="atpA"
                     /locus_tag="VFH91_06100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET6862596.1"
                     /translation="MEPIRASEINEIIRKQIQNFDTGVTVSEVGTVIKIGDGIAEIHG
                     LEKVMAGELLEFPHDVRGLALNLEEDKVGAVLFGDFQTIREGDLVKRTGRIMQVPVGD
                     ALVGRVVDVLGNPIDDAGPIITDHYYPVERIAPGVVERQPVKEPLQTGLKAIDAMVPI
                     GRGQRELIIGDRQTGKTAVAVDTIINQKGKDVICIYVAIGQKASTVAQVVKTLHDFGA
                     MDYTIVIKATASDPAAMQFLAPYSGAAMGEYFRDNGKHALTIYDDLSKQAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSEARGGGSLTSLPIIETQAGDISAYIPTN
                     VISITDGQIFLEADLFNSGFRPAINVGNSVSRVGGSAQIKAMKQVAGTLRLDLAQFRE
                     LQAFAQFGSDLDKATQAQLARGQRLTEILKQPQYKPMDVEKQVLVIWAATNGYTDDIA
                     VEDVRKFESDLLSFVESSHPGLLASIREKKSLTDEIKEDLKQALVDFKDMRNKPKPAS
                     VGSLSASPAREPAAVPVAAV"
     gene            2275..3162
                     /gene="atpG"
                     /locus_tag="VFH91_06105"
     CDS             2275..3162
                     /gene="atpG"
                     /locus_tag="VFH91_06105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET6862597.1"
                     /translation="MPNLLDIRRRIKSVKNTQQITKAMKMVSAAKLKRAQDRVVTARP
                     FANKMIEVLGQLAKRTDEDFRHPLLDLRGDERYLLILITADKGLCGAFNTNLTKAAQA
                     FIKENSDKSIELVAIGRKGRDFFRNRHATIASEYIGLTGKGRVDFSEALEVARAIIKL
                     YTETKEIDKVFLIYNEFKSVLSQRVVLEQLLPVSRAENEEPDSKSQQPVNLVDYIYEQ
                     PPAEMFAQLLPRLIETQIFRALLESVASEQGARMTAMDSASKNASELIDSLTLNMNRI
                     RQAAITNEIIEVVSGAAAL"
     gene            3248..3604
                     /locus_tag="VFH91_06110"
     CDS             3248..3604
                     /locus_tag="VFH91_06110"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET6862598.1"
                     /translation="MQAQPTSRILSRYVPLVVWLCFITYASSDEFSASNTSRIIGPIV
                     LWLFPNTSTETMTIIHLIVRKIAHFTEYGILGYLAVRAFRTSPGTYLSRYWFVISFSI
                     VVLYALIDEYHQSFVP"
     gene            3767..4909
                     /locus_tag="VFH91_06115"
     CDS             3767..4909
                     /locus_tag="VFH91_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET6862599.1"
                     /translation="MGILDRLSPSSQFIEVIEWLDDSSNTLLYRFPVKDQEIKNGAQL
                     IVRESQAAVFVHEGQIADLFLPGRYTVDGGNTPILSKLGAWKYGFNSPFKAEVYFVNT
                     KQFTDLKWGTPNPVMMRDSDFGMVRLRAFGIYSIRVADPKAFIKEIAGTNARFITEDI
                     EGQLKRTLVSGFSDALAESKIAALDLASNYDELSKFTRVKLNEEFTVFGLELTKFVIE
                     NISLPQEVEAAMDKRTSMGVIGDVGRYAQFQAADAMRDAAQNPGGGAGSGAGLGAGFA
                     IGNAMAGAMSDAINKSNTSARTGISCPKCGSQNTPGAKFCNECGAKIEASSSTVPCVK
                     CGSPLQAGTKFCNECGAKQEKQTCPNCKADVAPGAKFCNDCGQKIE"
     gene            5051..6319
                     /locus_tag="VFH91_06120"
     CDS             5051..6319
                     /locus_tag="VFH91_06120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET6862600.1"
                     /translation="MLRTLTRPRRVVITGMGCVTPLGVGREAFWTSLLEGKSGTRKIT
                     AFDPSTFSVQVAAEVPDFDWEAQLNPKDRKHVARTVPLALGAAREALEDAGLFPNDLS
                     LSERRVFGVELGTGGGGLAFTEQQYEYWYVGPTTKASVYTIPSSTHGGLSSELSMAFG
                     LRGLSHIVSTGCTSSTDALAYASQHIALGRQDMMLSGGVDAPIAPGILAAFNLMTVLT
                     NEWNDEPHRASRPFSRNRSGMVLGEGAWIYVLEELSHAKARGAKIYAEITGYGSTCDA
                     YHRVRLAEDGDEPARAMRLALEDAGRETKHVDYVNLHGTSTLLNDRIETSALKLAFDG
                     LAKRIPMSSTKSQIGHPQGASGAAGLGAALCAINRNKIPPTINLDEPDPSCDLDYVPN
                     VAREADVRIALCNCIGFGSKNSALVVERFV"
     gene            6362..7117
                     /locus_tag="VFH91_06125"
     CDS             6362..7117
                     /locus_tag="VFH91_06125"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6862601.1"
                     /translation="MFERFKSRSYEPENIDTGSYTADEYEGCLVELRRVNRWMGDSRS
                     LGHTLFRTVQAEALSSFSVLDVGAGSGELLRVTANWARQTNRGFRGVGLELNQRSVEA
                     IRDESLSYRELKAVRGDALNLPFLAQEFDYAICSLFTHHFHEDQIVRILEEMSRVARR
                     KIFVIDLHRHPMAYFLYTTVGKLILHTRLIREDGALSILKGFNKQELKTLGDRAKLEG
                     SVVDRHFPFRLVLSGSGNHSPLKRDTVVVDRAA"
     gene            7137..>7409
                     /locus_tag="VFH91_06130"
     CDS             7137..>7409
                     /locus_tag="VFH91_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6862602.1"
                     /translation="MSDYDVIIAGAGPAGSAAAIHLALSGFEVLLLEKQIFPRPKLCG
                     EFISPECQRHFEKLGVARAMELASPATLTRTSFYGRKGQYVEVPSSS"
BASE COUNT         1864 a         1791 c         1941 g         1813 t
ORIGIN      
        1 cagtgttcaa ctggctggtg agtcagttcg ccaaaacctc cgacctgaag acgatgttcg
       61 tttaatgact gaaagcatca gtggcctcag gaggaatcga gcttgagttt gcagacagtt
      121 gcccgccgtt acgcttccgc acttgctgac gtgataattg aacgacgtga agagcgtcag
      181 gttcaagacg aagtcctgca atgggcctcc atcatcgaag atcatgtgca acttcgcgaa
      241 gtgttcacaa atccgaccgt cgcttacgac cagaaaagac aactcctgga ggagttgatt
      301 actcgaacca gagttcgaga gacaaccgcc agctttttac gagtgttgtt aaagaaccag
      361 aggctcggtg aactcaaaga gatttctgtc aaactcgggc aagtcctcga tgaccgaagt
      421 ggcgttgttg cagctgtggt gacgacggca cgacctcttc ctgagaatct gaagtcgaat
      481 ctggaagaga gtttaactgc ttcagccggc cgcagagtgc ggctaagttt tgcgaccgac
      541 gagtcaatca ttggaggctt agtagcccgg atcggctcaa cgatttttga cggatctgtc
      601 caaagtcaat tggagcggct cgccgctgat ttaaccgatt agagccagag gatttgaaag
      661 accgcagagg acatcatgga accaatacgt gctagcgaga ttaacgaaat aattcgtaag
      721 cagatccaaa actttgacac aggcgttacg gtatctgagg tagggaccgt gataaagatc
      781 ggcgatggta ttgccgagat tcatggactc gaaaaggtca tggctggtga gctcctcgag
      841 tttccgcatg atgtgcgcgg ccttgcactc aatttggaag aagacaaagt aggtgcggtt
      901 ctgttcggcg actttcagac gattcgtgaa ggcgatttag taaagcgcac cggcaggatc
      961 atgcaggtgc cggttggtga cgcgctcgta ggtcgagttg ttgatgttct tggaaatcca
     1021 attgacgatg ctggtcccat catcaccgat cactattatc cagttgaacg aattgctccg
     1081 ggcgtagttg aaaggcagcc cgtaaaagaa ccattacaaa ctggtctaaa agccattgac
     1141 gcgatggttc caatcggccg cggtcagcgc gaactaatta ttggcgatcg acagaccggt
     1201 aaaaccgcgg ttgctgtcga cactataatt aatcagaagg gcaaagacgt tatttgtatt
     1261 tacgtcgcca tcggacaaaa ggcttcgacg gttgcccagg tcgtgaaaac tcttcatgac
     1321 ttcggcgcaa tggattacac cattgtaatc aaggccacgg cctctgatcc ggcggccatg
     1381 caattccttg caccgtattc aggtgcggcg atgggtgaat atttcagaga caacggtaag
     1441 catgccctga ccatttacga cgatctgtcg aaacaggcag ctgcgtatcg cgagatctct
     1501 ctgttgttaa gaagaccacc gggccgcgaa gcttatccag gcgacgtttt ctatcttcac
     1561 tcgcggttgc ttgaaagggc agcaaaattg tcagaagcga ggggtggcgg ctcgcttacc
     1621 agcttaccga tcattgagac ccaagccggc gacatctcgg cttatattcc gacgaatgtt
     1681 atttcgatta ccgatgggca gatttttttg gaggccgact tattcaacag cggattccgt
     1741 cctgcaatta acgtcggcaa cagcgtttct cgcgtaggcg gttcagctca gatcaaagca
     1801 atgaagcaag tcgctggaac gctccgtctc gacttggccc agtttcgtga gctccaggcg
     1861 tttgcgcagt ttgggtccga cctggacaag gccacgcaag ctcagctagc acgcggacag
     1921 cgccttacgg aaattctcaa acaaccacaa tataagccga tggacgttga gaagcaggta
     1981 cttgttatct gggctgcgac gaacggctac acggacgaca ttgctgtcga ggacgtgcgt
     2041 aagtttgaat ccgatctgct tagctttgtc gagagttcgc atcctggttt gctcgccagc
     2101 attcgcgaaa agaagagttt gaccgatgaa atcaaagagg atctgaaaca ggcgctggta
     2161 gatttcaaag atatgagaaa caaaccaaag ccggcctctg taggtagcct cagtgcatcg
     2221 ccggcacggg aacctgccgc tgttccagtt gctgcagttt aaccaaagac cagaatgccg
     2281 aatctcttag atatccgccg gcggattaag tcggtcaaga atacgcagca gatcaccaag
     2341 gccatgaaga tggtttctgc ggcgaagttg aagcgtgcgc aggaccgtgt ggtcacagca
     2401 cgtcctttcg cgaacaagat gatcgaggtg ttgggccaac tggccaagcg cactgatgaa
     2461 gactttcggc acccccttct cgatctacga ggcgacgagc gttacttact aattctcatc
     2521 actgctgata aaggtttatg tggagctttc aatacaaacc tcacgaaagc cgcgcaagct
     2581 tttatcaaag agaattccga taaatcgatt gagctcgtag caattggtcg taagggtcgc
     2641 gactttttcc gaaaccgtca tgcgactatc gcgagtgaat acatcggact tactggaaaa
     2701 ggccgcgtcg acttttctga ggcgttagag gtagctcgcg caatcatcaa gctctacact
     2761 gaaacgaaag agatcgataa agtctttcta atctataacg agtttaagtc ggttctctcg
     2821 cagcgagtcg ttctcgaaca actacttccg gtttcccgtg ctgagaacga ggagcctgat
     2881 agcaagtcac aacagccagt caatcttgtt gactatattt atgagcaacc tcccgcggag
     2941 atgttcgcgc agttattgcc acggttgatc gaaacccaaa tattccgagc actcctcgag
     3001 tcggttgcat ctgaacaggg tgctcgcatg actgctatgg actctgcttc gaagaatgcc
     3061 agcgaattga ttgacagctt gactctgaac atgaatcgaa tcagacaagc ggctattacc
     3121 aatgagatca ttgaagtcgt ctcgggtgca gccgcccttt aggaacggtc agcgaaaaag
     3181 acaaaaattc aatgggaagc ggaaaaccgc ttcccattgc tctttacaac gataaactct
     3241 ccggcaaatg caagcgcagc caaccagcag aatcttaagt cggtatgttc cattagtcgt
     3301 gtggctatgc ttcattacct acgcctcatc cgatgaattt tctgcttcca atacttcgcg
     3361 gatcattggc ccaatcgttt tatggctgtt tccgaacacc tcgacagaaa cgatgaccat
     3421 cattcacctg atcgtcagaa agatcgctca tttcaccgaa tatggaatcc tcggttatct
     3481 cgccgtccga gccttcagaa cttcgccggg cacctattta agtaggtatt ggtttgtgat
     3541 ttcgttttca atagtggtcc tttacgcctt gatcgatgaa taccatcaga gctttgtccc
     3601 ataacgtaca tcttcgattt acgatagcct tattgacatg accggcggtc taactgtgct
     3661 ccttttgttg agactctggt atcgtgaacg gcagccctca tcaatttcta cgatctgatc
     3721 gtataattct ccagtttcga cgattctgaa agttgaggta taggaaatgg gaatacttga
     3781 ccgactaagt ccaagttcac aattcatcga agtaattgaa tggttggacg attcaagcaa
     3841 tacgctccta taccggtttc cggtcaagga ccaggaaatt aagaatggcg cgcaacttat
     3901 cgtcagagaa tctcaggcgg ccgtcttcgt tcatgaaggc cagattgcag atctgtttct
     3961 accaggccgc tatacagttg acggcggtaa cactccaata ttaagcaagc tgggagcttg
     4021 gaaatacggc ttcaactcgc cgtttaaagc agaagtctat ttcgtaaaca caaaacaatt
     4081 tactgatctg aaatggggta cgccaaatcc tgtaatgatg agggattctg actttggcat
     4141 ggtccggctg agagcatttg gaatttattc catcagagtc gcggatccga aagcctttat
     4201 aaaggagatc gcaggtacga atgcgcgttt cataacggaa gacatcgaag gccaattgaa
     4261 gcggacactg gttagtggtt ttagtgatgc gcttgcggaa tccaaaattg cagcattgga
     4321 ccttgcttca aactacgacg agttgagcaa attcacccgc gtcaagttga atgaagaatt
     4381 cacagtgttc ggtttggaac ttaccaagtt tgttattgaa aacatctcgt tacctcaaga
     4441 agtcgaggct gcgatggaca agcgtacttc aatgggagtg ataggcgatg taggcagata
     4501 cgcgcaattt caggctgccg atgcgatgcg cgacgcggca caaaatcccg ggggcggcgc
     4561 cggttcgggt gcaggacttg gagccggttt cgctattggt aacgccatgg ctggagcgat
     4621 gtctgacgca atcaataagt caaatacttc agcccgaacg ggaattagtt gtccgaaatg
     4681 tggaagccaa aacactcctg gcgcaaaatt ttgtaatgaa tgtggcgcta agattgaggc
     4741 gtcaagctct acagtcccat gtgtaaagtg tggttcacca ttacaggccg gtacaaaatt
     4801 ctgtaacgag tgcggcgcta aacaggaaaa acaaacctgt cctaactgta aagcagacgt
     4861 agctccgggg gcgaagttct gtaacgactg cggtcagaaa attgaatagc tggtcgcgat
     4921 ggaccattcc cttctggtcc ctggcccaaa cccgtaacgc gaccgtctct tttgtgttgg
     4981 tccccatagc tgctatcttt aacctcccct ttgtagcgcc ccgtttttgg ccccgagaag
     5041 ggacggttgc atgcttcgta ctctgacacg cccgagacgt gtcgtgatta ctggaatggg
     5101 ttgtgtcact ccgcttggtg ttgggcgaga ggcgttctgg acttcactgc tcgaaggcaa
     5161 gagtggcaca agaaaaatca cggcgtttga cccgtcgaca ttttccgttc aagttgctgc
     5221 ggaagttccc gacttcgatt gggaagcgca acttaatcct aaagaccgca aacacgtcgc
     5281 tagaactgtt cctctggctt tgggcgccgc ccgtgaagca cttgaagacg cgggtctctt
     5341 tcctaatgac ctttcattgt cggaacgtcg cgtgtttggc gttgaactcg gaacaggcgg
     5401 cggcggcctg gcctttactg aacagcaata cgaatactgg tacgttggtc caactacaaa
     5461 ggccagtgtt tacactattc cttcatcaac tcacggcggt ttgtccagtg agctatcgat
     5521 ggcgtttggc ctgcgaggct tatcgcacat cgtttcaacc ggctgcacaa gttccacgga
     5581 cgcactcgct tacgcctccc aacacattgc cctgggacgc caggacatga tgctttccgg
     5641 tggtgtcgac gcaccaatcg cccccggcat ccttgccgcg ttcaacttaa tgaccgtgct
     5701 gacaaatgag tggaatgatg agccccatcg cgcgtctcgt ccattttcac gaaatcgatc
     5761 cggaatggtg cttggtgagg gcgcctggat ttacgttctt gaagagttgt cgcacgcgaa
     5821 ggctcgaggg gctaagatct acgcggagat tactggttac ggctcaacct gcgatgctta
     5881 tcatcgcgtg cgcttagcag aagatggaga tgagcccgca agagccatgc ggttggccct
     5941 cgaagatgcc ggccgagaga ctaaacacgt tgattatgtg aacttacacg gcacgtcgac
     6001 attgctcaac gacagaatcg aaacgagtgc cttgaaactg gcgtttgatg gcctcgcaaa
     6061 gcgaattcca atgtcgtcca caaagtctca aataggtcat ccccagggcg caagcggtgc
     6121 agcaggtctc ggtgccgctc tttgtgcaat caacagaaac aagattcctc cgacaatcaa
     6181 tttggacgag ccagatcctt cgtgcgatct ggattacgtc cctaacgttg ctcgcgaagc
     6241 cgacgtgcga atcgctctct gcaactgcat cggcttcggg tccaagaaca gtgcgttggt
     6301 agtcgagcgc tttgtttagc caatttgagg agtgaatttt ccattcgact tggaccagcc
     6361 aatgtttgaa cgattcaaat cgaggagcta cgaaccggag aacatagata ccggcagcta
     6421 cacggcggac gaatatgaag gatgcctggt tgagttacga cgtgttaatc gatggatggg
     6481 cgactctcga tctcttggac acacgttgtt ccgaactgtg caggcagagg ctttgtccag
     6541 cttctcagtt ctcgacgtag gcgccggttc cggagagtta ttgcgtgtaa cagcaaactg
     6601 ggcgcgtcaa acaaacagag gtttccgtgg tgtcgggctc gagttgaacc agcgttcggt
     6661 cgaggctatc cgagacgagt cgctttccta cagggagtta aaagcggttc gcggagacgc
     6721 tttgaacttg ccgttcctgg cacaagaatt tgactacgct atctgctcat tatttacaca
     6781 tcactttcat gaagatcaga ttgtgagaat tcttgaagaa atgagtaggg tggcgcggcg
     6841 gaagatcttc gtcatagact tacatcgcca cccgatggcc tacttcctct acacaactgt
     6901 tggaaagttg attctccaca cacgtttgat tcgtgaagat ggagccttat ccattctgaa
     6961 agggttcaat aaacaggaat taaagacttt gggcgataga gcgaagcttg aaggcagtgt
     7021 tgtggatcga cactttccgt ttcggttggt tctgagtggt tcgggtaatc actcccccct
     7081 caagagagac acggtggttg tagaccgtgc cgcgtgatag ttgatggtgg gccataatga
     7141 gtgattacga tgtgataatt gcgggagcgg ggccggcggg aagtgcagcg gcgatacatc
     7201 ttgctctctc aggttttgaa gtactccttc tcgagaaaca aatttttccg cgaccaaagt
     7261 tgtgtggtga attcatctca cccgaatgtc aaagacactt tgaaaaactg ggagttgcca
     7321 gagcgatgga gctcgcaagt cccgcgacgc tcactcggac ttcgttctac gggcgaaagg
     7381 gccaatacgt tgaggttccc agtagttct
//
LOCUS       DASSFP010000277         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1563172, whole genome shotgun sequence.
ACCESSION   DASSFP010000277 DASSFP010000000
VERSION     DASSFP010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563172"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFH91_06135"
     CDS             <1..596
                     /locus_tag="VFH91_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862603.1"
                     /translation="NYGVKTDMLMDAPTIANLKNSAIYIIVDPDTKEETPTPNFIEPK
                     DVKAISDWVRAGGVLVLMGNDVGNAEFDHFNQLAAVFGIQFNKDSRNKVPGNDFAMGK
                     LTVVPPHPIFKTARNLYLKEISTLQLKPPARAVFTEGGDNIMAVAKFGKGTVFAVGDP
                     WLYNEYTDGRKLPPEYDNYKAANDLALWLIQQTQGKL"
     gene            617..1354
                     /locus_tag="VFH91_06140"
     CDS             617..1354
                     /locus_tag="VFH91_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET6862604.1"
                     /translation="MSDSNAVFKTIDAERRGTTSEEVVAQLREMIHKGELRPGDRLPP
                     ERDLAKLLGVSRPTLRAGISSLAAVGVLQPRRGAGTFVVEAEGSPALDSSPLRLMASL
                     QRFTPAEMFEARRSLEMAIAGLAAERATGDQMASMSEEVTGMFASLDEPEQFLVHDMR
                     FHQTVAAASGNRILTALMNMVATILFEVRSKTVKRARDLKESAEMHRRIYRAIRDRNA
                     DAARDAMRDHLMLAQKAQEEEEKLSSK"
     gene            1546..3174
                     /locus_tag="VFH91_06145"
     CDS             1546..3174
                     /locus_tag="VFH91_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HET6862605.1"
                     /translation="MYSHLSRACAIVILILSLSGVVRSQSEQTVWDPDNGNGTYTNPI
                     IHADYSDPDVIRVDDDFYLTSSSFNSAPGLPILHSKDLVNWKIIAHVFQQQIPGDVFA
                     RPQHGNGVWAPALRYHDGYFFIFYPDPDYGIYMSKARNAAGPWSAPLLIKSAKGWIDP
                     CPFWDTDGNAYLVHAWANSRSGIKSILTINKMSADGTKILDEGRTVFDGRSNQPTIEG
                     PKLYKRNGYYYIFAPAGGVPTGWQTVLRSRNIFGPYQARVVMDQGKTQINGPHQGAWV
                     QLKSGESWFIHFQDKGPYGRVVHLQPIRWRNNWPVIGRDANHDGKGEPVPTYKKPAVA
                     QQDTRQAPQTTDEFDSPEIGLQWQWQANFDDRWWTLNARPGWLRLNAVPLPREAVNLW
                     PVPNLLLQKIPAPAFTLTTRIDANSLSIGEEAGLVLMGMDYSYLAVVRNESGFRLEKT
                     ICKDANSGVEESEEAGLNIAAGLLHLRMTMTKDGIARFSFSSDGNKFIPIGEPFKARE
                     GKWIGARVGLFCLAMSEGPQHGYADFDWFRFERS"
BASE COUNT          887 a          835 c          930 g          850 t
ORIGIN      
        1 ggaactacgg cgtcaagact gacatgttga tggatgcgcc aactatcgcg aacttgaaga
       61 attcagcgat ctacatcatc gtagatccgg acaccaaaga agagacacca acaccaaact
      121 tcatcgaacc aaaggatgtg aaggcgatta gcgattgggt cagagccgga ggcgttctag
      181 tgttgatggg aaatgatgtg ggtaacgcgg agtttgatca tttcaatcag ttagcggcag
      241 tgtttggaat tcaattcaac aaagacagtc gtaacaaagt gcctggtaat gactttgcta
      301 tgggcaagct cactgtggtt ccgcctcatc cgattttcaa aacggcacga aatctctatt
      361 tgaaggagat ttctactctg cagttgaaac cgcctgctcg cgctgttttt accgagggtg
      421 gcgataacat tatggcggta gcgaagttcg gaaagggcac tgtttttgcc gttggcgatc
      481 cctggcttta taacgagtac actgatgggc gaaaattgcc gccagagtac gataactaca
      541 aagccgccaa cgatcttgcc ctctggttaa ttcagcaaac gcaagggaag ttgtgaaggt
      601 gtagactgag tcaactatga gcgattcgaa tgccgtgttc aagacgatcg atgcggagcg
      661 gcgtggcact acgtcagaag aggtcgtagc gcagcttcga gagatgatcc ataaaggcga
      721 attgcggccg ggagatcgac tgcctcccga gagggatctg gcaaagctac tcggagtgag
      781 tcgcccaacg ttacgcgctg gaattagttc gttggcagca gtcggagtgc ttcagcctcg
      841 tcgcggggcg gggacattcg ttgttgaagc agaaggttca ccggcgctcg acagcagtcc
      901 gctgcggctc atggcttcgc ttcaacgatt tactcccgcg gaaatgtttg aggcaagacg
      961 ttcactcgaa atggcgatcg ctggtctggc tgctgaacgc gctacgggag atcagatggc
     1021 ttcaatgtcg gaagaggtca ctggcatgtt tgcttcgctc gacgagccgg aacagttttt
     1081 ggtccacgac atgcgcttcc atcaaacagt ggcggcggcg tcaggaaacc gtatattgac
     1141 ggcgttgatg aatatggttg ccacgatttt gtttgaagtt cgaagcaaga cggtcaagcg
     1201 cgctcgtgac ttaaaggaat ctgccgagat gcatagacgt atctaccggg ccattcgaga
     1261 tcgaaatgcc gatgcggctc gtgacgcgat gcgcgatcac ctgatgctgg cccagaaagc
     1321 tcaggaggag gaagagaagc tctcctctaa gtagtgaagt gtgcatcttt ccgattttct
     1381 cttagctcca ggtgaccccc aatcgcgttg gaggataaaa ccagcaacgt tccccgcgtc
     1441 gcgtcttgat tcctttgcgc tacaagaaaa agacaagccg tcgaaacccc atctctaaat
     1501 attgcatcaa accagcctaa agcggtcaga ctgagaaacc taataatgta ttcgcatctt
     1561 tctagagctt gcgcgattgt gatcctgatc ctgtccctct ccggcgtggt ccgttctcaa
     1621 tctgaacaaa ctgtttggga tcctgataat ggaaatggaa catacacaaa tccgatcatt
     1681 catgccgact attccgaccc ggatgtgatt cgcgtcgacg acgatttcta tctcacttcg
     1741 tcaagcttta attcagctcc ggggcttccg atacttcatt cgaaggatct tgttaattgg
     1801 aagatcattg cgcacgtatt tcagcagcaa atacccggag acgtctttgc caggcctcaa
     1861 cacggaaatg gagtatgggc gccggcgctg agatatcacg atggttattt ctttatcttt
     1921 tatcccgacc ctgactacgg catttacatg tcaaaagccc gtaatgccgc cggtccatgg
     1981 tccgcgccat tactaatcaa gagtgctaaa ggctggatcg atccttgtcc attctgggac
     2041 acggatggaa atgcctattt ggtgcatgcg tgggccaaca gtcgatcagg catcaagagt
     2101 attttgacga ttaataagat gagtgcggac ggaacgaaga ttttggacga aggccggact
     2161 gtttttgacg gacgttcaaa tcagcccacc atcgagggtc caaagctcta taagcggaat
     2221 ggctactact acatctttgc gcccgctgga ggtgtgccga ctggttggca aacagtttta
     2281 cgatccagga atatctttgg tccctatcag gctcgagtgg tcatggacca gggaaagaca
     2341 cagattaatg gaccccatca aggtgcgtgg gtacagctaa aatcgggtga gtcgtggttt
     2401 atccattttc aggacaaagg accttatggc cgtgtggttc atctgcagcc gatacgatgg
     2461 cgtaacaact ggcccgtgat cggtcgcgat gctaatcatg acggaaaagg cgaacctgtg
     2521 cctacctaca agaagccggc ggttgcgcaa caggacactc ggcaagctcc acaaacaacg
     2581 gatgaattcg actcaccaga aattggtttg caatggcaat ggcaagcaaa cttcgatgat
     2641 cgctggtgga cattaaatgc tcgaccggga tggttgaggc tcaacgcagt tccgcttcca
     2701 cgtgaagccg taaacctttg gcccgtacca aatcttttac ttcagaagat ccccgcgcct
     2761 gcgttcactc ttacgacaag gatcgatgcc aacagccttt cgattggtga agaagcaggt
     2821 ctcgtgttaa tgggaatgga ttattcatac ctggctgttg tgcgcaacga atcgggattt
     2881 agactcgaga aaacaatctg caaggatgcg aattctggag ttgaagagtc ggaggaagct
     2941 gggctgaata tcgcagctgg tttacttcat ctacggatga cgatgactaa ggatggaatt
     3001 gcgcgattta gtttcagcag tgacggaaac aaattcatcc cgattggtga gcccttcaaa
     3061 gcccgtgaag gcaagtggat aggagcgcgg gttggattat tctgtcttgc gatgtccgaa
     3121 gggccgcaac atggatacgc ggacttcgac tggttccgat ttgaacggag ttaaattcca
     3181 tcaacgggtt ggttgtttgt aggctggact ttaacgcacc accaactgcc gttggtggaa
     3241 ttcgatcaat tagtaactct caattatcaa ttcataattc tcaaatatta agggtatgtg
     3301 gtaagtagac tgcgcccaat tagtaatctc aaatacccct ggcttcccga acgatctcga
     3361 catactgctt tgcgcgctca gtgatgacgc tgagttgtcc atcacgaatg gccttgaggt
     3421 ccaccaggtc ggctccaaca cctaaggcac tcgctccggc cttgatgaaa tctgccgcag
     3481 tcttcatcga cactccacca gt
//
LOCUS       DASSFP010000278         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1267558, whole genome shotgun sequence.
ACCESSION   DASSFP010000278 DASSFP010000000
VERSION     DASSFP010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267558"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFH91_06150"
     CDS             <1..429
                     /locus_tag="VFH91_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862606.1"
                     /translation="TIFKVNGIGEEKAWVQVEPSVSAYHLAFGPDSFLYLTGPTVTSF
                     DSVWRISPEGEVETFFRGLGRPQGLAFDAAGNLYVAAALRGRRGVVRITPDGTSAEVV
                     VGGMNLVGLAFGPQGDLAVASIDSVYSVPVQIEGALLSGR"
     gene            561..1208
                     /locus_tag="VFH91_06155"
     CDS             561..1208
                     /locus_tag="VFH91_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6862607.1"
                     /translation="MVVLLLAISAHEAAHAWMSYKFGDDTARLLGRITLNPLAHTDPI
                     GTLLIPIVSFIFSAMGGGLGRIPLIGWGKPTPVNPLRWRNKDLANVMVSIAGILANTL
                     IAIIAFAILKVLLVSGLFFSMSDSIQEPVGLFLQYLLTMNISLAVFNLLPFPPLDGGK
                     ILETFLPASMQPVLETMERYGFIILMLLIYLGVTRAIIQPILSVVYYLLLMGVTN"
     gene            1223..2215
                     /gene="trpS"
                     /locus_tag="VFH91_06160"
     CDS             1223..2215
                     /gene="trpS"
                     /locus_tag="VFH91_06160"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET6862608.1"
                     /translation="MPKRIFSGAQPTGNVHLGNYLGALRNWVALQHEYESFYCIVNLH
                     ALTVPQDPRLLAEKTRDLARIYLAVGIDPKVATIFIQSDVPEHSELTWVLNCIARMSE
                     LERMTQFKDKAKKQQENVSVGLFDYPVLMASDILLYQTDLVPVGDDQKQHLELTRDIA
                     IRFNRDYGETFRVPDPYIPKVGARIMSLSDPSKKMSKSDDDPNGCVMLMDDAATIQKK
                     FKRAVTDSGTEIRFDESRPAINNLLEIFHLLTGKSQPEVESHFAGKGYAQLKGDLADA
                     AVGFLEPIQSAVRGISDDELDSILAEGRDKAQQIAATTLSHVFENMGIRGGGRR"
     gene            2225..3025
                     /locus_tag="VFH91_06165"
     CDS             2225..3025
                     /locus_tag="VFH91_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET6862609.1"
                     /translation="MSDETPEVAVESTDQAPVPLNVAGAVPQIVSDDTTDDLKVLLGE
                     FEGPLDLLLHLIRQEQVSIYDIPVARITDEYLRYLRVMQEMDIAVAGDFLVMAATLIE
                     LKTKMLLPRDPQAAAEEADDPRSELVNQLLEYQKYKAAAEMLWSRATVEQAVFKRAQI
                     ETDKNNPEVSVGLFDLLKVFQDILARHKEEVLMEIEREEISMAEMLERLRNMVMSASE
                     LNLRVYFERAKSRRELVLAFLSVLELVRTTEIKLFQRETFGDIIAKVG"
     gene            3037..3738
                     /gene="scpB"
                     /locus_tag="VFH91_06170"
     CDS             3037..3738
                     /gene="scpB"
                     /locus_tag="VFH91_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET6862610.1"
                     /translation="MSDDQDKAISNEPVDQVALNEEASQELAAMVAEAEASSKPDSES
                     EADDDGLLYSLKSQAERAAVIEALIFVSEEPLSTKIIADVLKEDREVVTQALEALVQE
                     FNARNSGLQLREVAGGWQFATRAEYHEHVRAFLKTRPSAKLTIASLETLAVIAYRQPV
                     TVPEILEIRGVQSPSAIKTLLDKKLIVAKGRKEAVGRPMMYGTSKEFLLQFGLKDLSE
                     LPSVEDFHDLSGGGS"
     gene            3759..4532
                     /locus_tag="VFH91_06175"
     CDS             3759..4532
                     /locus_tag="VFH91_06175"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6862611.1"
                     /translation="MEQRLQKLIATAGIASRRHAEELITLGRVTVNGKVITELGTKAD
                     PDKDHIKVNGKLINSQLRAHEKVHVLLYKPRGYLSSVSDPEGRPLVTELLPASLGRLH
                     PVGRLDFNTEGLLLLTNDGDFTNFITAARNRVEKIYEAKVKGVPTEAAIQRLRRGVTL
                     DDGTRTAPATIDKLDETATNSWFEVRLHQGRNQQIRRMFEVIGHSVLKLRRVQIGYLR
                     DDRLKPGHWRFLSDAEARRLLRKSGPKKVASKARRANHV"
BASE COUNT         1188 a         1124 c         1235 g         1183 t
ORIGIN      
        1 acgattttca aggtaaacgg aatcggtgaa gagaaagcgt gggtccaggt tgagccgtct
       61 gtatccgcct atcatctcgc gttcggtccc gattcttttc tttatctcac gggcccgact
      121 gttactagtt tcgattctgt gtggcgcatt agtcctgagg gtgaggtcga gacttttttc
      181 agagggctcg gtcgtccaca ggggcttgcg ttcgatgctg cgggaaatct ctacgtggca
      241 gcagcactcc gtggacgtcg aggtgtcgtc aggatcacac ctgacggaac cagtgccgaa
      301 gttgtagtcg gtggcatgaa tctggttggc ctcgcctttg gaccacaagg cgacctcgca
      361 gtggcctcga tcgactcagt ctactccgtc ccagtacaaa tcgaaggcgc gctcctctcg
      421 ggccgataaa agctgattcc ccgtagccaa tcacttttga cactacttaa gcgtaatgct
      481 actttgaccc tttatttctc ggctttttac tgggtgttta attgaatcca gaacaactaa
      541 ttcccaacct tattatctat atggtggtgt tgctgctagc tatctcggcg cacgaggcag
      601 cgcacgcctg gatgtcctat aagttcggtg acgatacagc ccggctgcta ggccgaatta
      661 cgctcaaccc gctcgcccat accgacccaa ttggtacgct gctgattcct atcgtcagtt
      721 tcattttcag cgcgatgggg ggaggtcttg gccgcattcc attgattggc tgggggaaac
      781 ctactcccgt aaatccacta cgatggcgta acaaggatct cgcaaacgtc atggtgtcga
      841 ttgcgggaat acttgccaat acactgattg cgattattgc gttcgctatt cttaaggtgt
      901 tgttggttag cgggctattc ttttcaatgt ctgattcgat tcaagagccg gtaggattgt
      961 ttttacagta tctcctgacg atgaacatct cattggctgt tttcaacctt ttgccatttc
     1021 ctccgctgga tggtggcaag attctcgaga catttttgcc cgcaagtatg cagcccgttc
     1081 ttgagaccat ggaacgctat ggcttcataa tcttaatgct cttgatctat ttaggagtca
     1141 ctcgcgcaat cattcaaccc attctctcgg tcgtctacta cttgttattg atgggtgtga
     1201 cgaactaatc gatttaaact taatgccaaa gcgaatcttc agcggcgccc aaccaactgg
     1261 caacgtacat ctgggaaatt atcttggcgc gctgcgtaac tgggtcgcgc tgcaacatga
     1321 atacgaaagc ttttactgca tcgtcaatct gcatgctctc acggttccgc aggatcccag
     1381 gttgttggct gagaagactc gcgacttagc tcgaatctac cttgccgttg gcattgatcc
     1441 gaaggttgcc acaatcttca ttcagtctga tgtgcctgag cattctgagc tgacctgggt
     1501 gcttaattgc atcgctcgaa tgtccgagct tgagcgcatg acccagttca aagacaaggc
     1561 aaagaaacag caggaaaacg ttagcgttgg attgtttgat tatccggtat tgatggcgtc
     1621 agacattttg ctgtatcaaa cggatctggt tcctgtgggt gacgatcaaa agcaacacct
     1681 cgagttaact cgtgacatcg cgatccgctt taaccgggat tatggcgaga catttcgtgt
     1741 tcctgaccct tatattccga aggttggagc gcgaataatg tcgctttctg atccgtcgaa
     1801 gaagatgtcg aagtcggacg atgatccgaa tggctgcgtt atgttgatgg acgacgcggc
     1861 gacgattcaa aagaagttta agcgagcagt aactgattca gggactgaaa ttcgtttcga
     1921 cgaatcacgg ccagcaatta ataatcttct ggaaatcttt catttgctca ccggaaagag
     1981 tcagccagaa gttgaatcgc actttgcggg taaaggttac gcgcaattga aaggcgatct
     2041 agccgacgcg gcagtcggat tcctcgagcc catacaaagt gctgtacgcg gaatctctga
     2101 tgatgaactt gattcgattc tcgcagaggg acgtgataaa gctcaacaga ttgccgcgac
     2161 gactttgtcc catgtctttg agaacatggg cataagaggc ggtggaagac gttaaagaag
     2221 tactatgagt gacgaaacac ccgaagtcgc ggttgagagt acggaccagg ctccagttcc
     2281 ccttaatgta gccggcgccg tgccccaaat cgtttccgac gatacaactg acgatctaaa
     2341 ggtgctactc ggcgaatttg agggtccgct cgatctactg ctgcacttaa tccggcagga
     2401 gcaggtaagt atttacgata ttccggtcgc gagaattact gacgaatatc ttcggtatct
     2461 cagagtaatg caggagatgg atatcgccgt tgccggcgac tttctcgtaa tggctgcgac
     2521 gctcattgaa ctgaaaacga agatgttgct gccgagagat cctcaagccg cagcggagga
     2581 ggcagacgac ccgcggtcgg aactagtaaa tcaactgctc gagtatcaga aatacaaagc
     2641 tgccgccgag atgttgtggt cacgcgctac tgttgaacag gcggtgttca aacgcgcgca
     2701 gatcgaaacg gataaaaata atcccgaagt gtcagttggt cttttcgacc tgttgaaagt
     2761 ttttcaggac attctcgctc gacataaaga agaagtcctg atggagattg agcgcgaaga
     2821 gatctcaatg gcggaaatgc ttgaacgatt gcgcaacatg gttatgtcag ccagcgaatt
     2881 aaacctgcgc gtctatttcg aacgagcaaa gtcacgcagg gaattggtcc ttgcatttct
     2941 gtctgtactt gaactcgtca gaacgacgga gattaagtta tttcaacgcg aaacgttcgg
     3001 cgacatcata gccaaagtgg gctgagagta acttcagtga gcgacgatca agacaaggca
     3061 atttcaaacg aacccgttga tcaggtggca cttaacgaag aagccagcca ggaactcgca
     3121 gccatggttg ctgaggctga ggctagttct aaacctgatt cggaaagcga agcggatgat
     3181 gatggtctat tgtattcgct aaagagtcag gctgagcgtg ctgctgtcat cgaggctcta
     3241 atctttgtct cagaggaacc tttgagtacg aagattatcg ccgacgtttt gaaggaagac
     3301 cgagaagttg taactcaggc cctcgaagcg cttgttcagg agttcaatgc tcggaacagt
     3361 ggtctacagc tgcgcgaggt tgccggtgga tggcagtttg caactcgggc ggagtatcac
     3421 gaacacgtgc gagcgttttt gaagacgcga ccgagtgcta agttgacgat cgcttcactc
     3481 gaaactcttg cggtcatcgc ttaccgtcaa ccagttaccg ttccggagat attggagatt
     3541 cgcggagtcc agtctccctc cgcgatcaag acgttgctcg ataagaaact gattgtcgca
     3601 aagggcagga aagaagcagt aggtcgtccg atgatgtatg gaacctcgaa agagttcctc
     3661 ctgcagtttg gcttgaaaga tttatcggaa cttccaagcg ttgaagactt ccacgatcta
     3721 agtggcggtg gatcctagta ctggtcaatt atcatttagt ggaacagcgg ctacaaaaac
     3781 taattgctac ggccggaatt gcctcgcgga gacacgccga agaactgatc acacttggac
     3841 gagtaactgt caatggaaag gtcatcaccg aattaggaac aaaggctgat cccgataaag
     3901 atcacataaa ggtaaatggg aagctcatca attcccagtt gcgagctcac gaaaaggtgc
     3961 acgttctgct ctacaaacca cggggctatt tgtcgagtgt ttctgaccct gaaggccgcc
     4021 cgttggtaac cgaattgctt ccggcttcac tcgggcgact gcatcctgtc ggtcgacttg
     4081 atttcaacac cgaaggactc ctgcttttaa ccaacgacgg cgatttcacc aactttatta
     4141 ccgccgctcg caatcgtgtc gaaaagatat atgaagcaaa ggtcaaaggc gttcccactg
     4201 aggcagcgat tcaacgcctg agacgaggtg taacgctcga cgacggtact cggacagctc
     4261 ccgcaactat agataaactc gacgaaacgg ccacaaacag ctggtttgaa gtgcgcttgc
     4321 atcagggacg caaccaacag atacgccgaa tgtttgaagt gattggtcac tcggtgctga
     4381 aattgcgaag ggtgcaaatt ggatacttga gagacgatcg cctaaaaccc gggcactggc
     4441 gtttcctgtc cgatgctgaa gcccgcagat tgcttcgcaa atcaggaccc aaaaaggtcg
     4501 cctcgaaggc gcgtcgcgcg aatcacgttt gacggcgact ttagggtaaa atctattact
     4561 caaattgagg tcatcagggt ggcatttcta gtctgtcggc tgacttggac agttaggaat
     4621 gccaacgttg tgtaaggaac aaactttatg gattttgagt taaacgaaga gcagcaacag
     4681 atcaagatga gcgttcgcga gtttgctcag tccgaaatcg cccctcacgt
//
LOCUS       DASSFP010000279         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1850241, whole genome shotgun sequence.
ACCESSION   DASSFP010000279 DASSFP010000000
VERSION     DASSFP010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850241"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..459
                     /locus_tag="VFH91_06180"
     CDS             127..459
                     /locus_tag="VFH91_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6862612.1"
                     /translation="MAKDKADLLQGTLDMLILKALSLGELHGYGIIQRIRQLSDEMLN
                     VEQGSLYPALYRIEQKGWVSSKWSVHDTGRDAKFYKLTRSGRKQLQDQEASWDRLVLA
                     VAKVRQAT"
     gene            473..>2277
                     /locus_tag="VFH91_06185"
     CDS             473..>2277
                     /locus_tag="VFH91_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862613.1"
                     /translation="MKWFNLFRDRIRALSGRDGVIEEIDREMRAHVEMQTEANISSGM
                     DPVQARDEALRSFGHLTNIRDDAYDVKGGGFIETLMQDIRYGTRILTKHRAFTAVAVL
                     TLALGIGANTAIFSVVNELLLRPMAYRDADKIVMVWEVTPEGRHQNTTSRANFRSWRE
                     QASSFENISAFTDQRLNLTGEGEPEELSVQFATPELFKILGVDPILGRPLMPEDADVR
                     NQIAVLSYGLWQRRFGGRLDIVGQSITLNGVPFSVVGVMPASFQFHIKQRSGTGRPPE
                     LWSVLSMRTGALANDRGRFLSVVGRLKPGVSVDQANAEMRTIATRLSADAPQFNFNFS
                     AEVLPLREQFFGNVRRALWLLLGAVGFVLLIACANVANLLLSLATSREKEIALRAALG
                     ARRVRIVRQLLTESLMLAIISSLMGLGFAWLGIKALIAISPKDLVSLQNTNLDLTVLL
                     WTLGVSVLTGIVFGLAPALHITRLDLNDALKEGGKGDTGQATGSRKLRSLLVVSEIAL
                     AVVLLASAGLLVKSFIRLQEVDRGFNSGNVMTMVVRLPQSKYKEDQHVVDFFRQALDR
                     TNTLPGVRSAGVINYLPFYGGLGSSTGFTIGGRPAP"
BASE COUNT          543 a          548 c          612 g          574 t
ORIGIN      
        1 ttctacgaac tttagagaca ggacactagc tcaatctcaa tactttgaca atctaggcat
       61 catagattta tgatgcctcg cctttctagg catcacgcta aaatctgatt ggtgggacaa
      121 gtaatagtgg caaaagataa agccgatctg ctgcaaggca ctctcgatat gttgatcctg
      181 aaagcactgt cactcggaga actacacggg tatggaatca ttcaacgaat tcgtcaattg
      241 tccgacgaaa tgttgaatgt cgaacaaggc tccctctatc ccgcgctcta tcggattgaa
      301 caaaagggtt gggtgtcgtc gaagtggtcc gtccacgaca caggacgaga tgccaaattc
      361 tataagctga caagatcggg tcgaaagcaa ctgcaagatc aagaggccag ctgggatcgc
      421 ctcgtactcg ccgtcgctaa agtcagacaa gcaacgtaga ggtaatccat ccatgaagtg
      481 gttcaatcta ttccgcgacc gaatacgcgc attgagtggt cgcgacgggg taattgaaga
      541 aatcgaccgc gagatgcgcg cgcatgttga gatgcagacc gaagcaaaca tctcatcggg
      601 gatggatccg gttcaggcca gagacgaagc ccttcggagt tttggtcacc tgacaaacat
      661 tcgagacgat gcttatgacg tcaagggtgg tgggttcatc gaaacgctaa tgcaggacat
      721 ccgctacggc actcgtattc tgacaaaaca tcgagcattt actgcagttg cagttctcac
      781 cctggcgctg ggaattggcg ccaacacagc aatcttttct gttgtaaacg aacttctctt
      841 acgaccgatg gcttatcgtg acgccgataa gatcgtaatg gtatgggaag taacgcctga
      901 gggcagacat caaaacacaa cctcgcgagc taacttccga agttggcgcg aacaagcatc
      961 gagttttgaa aacatctctg cctttaccga ccaacgactc aacttaactg gtgaaggaga
     1021 acccgaagag ctttcggttc agtttgctac acccgagcta tttaagattc tgggagttga
     1081 tcccattctc gggcgtcccc taatgcccga agatgcggac gtgaggaatc aaatcgctgt
     1141 tttgagctat gggttgtggc aacgccggtt tggaggacgg ttggacattg tgggccaatc
     1201 gatcactctg aacggagtac cattttctgt cgtgggagtg atgcccgcaa gcttccagtt
     1261 tcatatcaaa cagcgttcgg gtaccggcag gccgccggaa ctctggagtg tgctgtcaat
     1321 gcggactggc gccttagcga atgatcgtgg gcggttcctg tctgttgttg ggcgtcttaa
     1381 accaggagtt tcggtggacc aggcgaatgc cgagatgcga acaattgcca ctcgtctatc
     1441 ggcagacgct ccacaattca acttcaattt cagcgcagag gtactgccgc tacgtgaaca
     1501 attttttggc aatgtccggc gcgccctttg gcttttgctg ggtgcagttg gttttgttct
     1561 gctgattgcc tgtgcgaacg tggctaacct tttactgtct ttggccacct cacgtgagaa
     1621 agagatagct ctccgggccg ctttaggtgc gcgtcgcgtg agaattgttc gacaacttct
     1681 gactgaaagt ttgatgctcg ctattatcag cagcttgatg gggcttggct tcgcatggct
     1741 tggcattaag gctttgattg ccatcagccc taaagacctc gttagtttgc agaacacaaa
     1801 ccttgatctc acggtgttgt tatggacttt gggagtgtct gttcttactg ggatagtttt
     1861 cgggcttgcg cctgcattac atatcacacg tcttgacctt aacgacgcct tgaaggaagg
     1921 cggtaagggt gacacgggcc aggcaactgg aagtcggaag ctacgcagcc tgctggtggt
     1981 ctcggaaatt gcgcttgctg tcgttctgct ggccagcgcc ggcttattgg taaagagttt
     2041 tatacgactc caggaagtcg atcgtgggtt caactccggc aacgttatga ctatggtcgt
     2101 gcgtcttccc caatcgaagt acaaagaaga tcagcacgta gttgatttct ttcgccaggc
     2161 attggaccgc acaaatactt tgcccggagt gcgttcggca ggtgtgatca actatctacc
     2221 gttttatggc gggctagggt cctcgacggg atttactatc ggcggcagac ctgcacc
//
LOCUS       DASSFP010000280         7309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1108950, whole genome shotgun sequence.
ACCESSION   DASSFP010000280 DASSFP010000000
VERSION     DASSFP010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108950"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFH91_06190"
     CDS             complement(<1..299)
                     /locus_tag="VFH91_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862614.1"
                     /translation="MILTNKLLPVALIAALIGGSVGAIVMYKSQPATAETVAQNTTPT
                     NTLPAEYQTGESVPGSFNTPAEQTAYKTGFADGFSAASQTGTTTSRASAPTRVVSR"
     gene            complement(657..1964)
                     /locus_tag="VFH91_06195"
     CDS             complement(657..1964)
                     /locus_tag="VFH91_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6862615.1"
                     /translation="MSLRTKFILILASATLTIYTVVGGWISTRAQQPANDPNAQLRIF
                     ESVLQHIQSDYVDEPNMEKVRAGALRGLAYGLDPYSTYLTPEQVREYNGNTSKDAPSG
                     IGAELSQVASYLYVIAPLKGSAAEQAGIRAGDIIEYIDGKATRDISLYDAKQLLNGAA
                     GTEVKLRVLRANSNPLTLTVKRGTNRAPATEARMDAGRVGILRLNSLGDGEANDTKAK
                     LQDLVKQGAQKIVLDLRDTAGGSLDEAVAVANFFIKDGVLAQTVGREGKALKTFTADP
                     KAVIFNGPVVAMIDTGTAGASEVIASALLEHNRGQVVGEKSFGAGAEQQLFTLRGGDG
                     LLLTTVKWASGNGKAFLGEDRNHSGVSPSVEVKGPEIADSVDPDDLTGNDDDGVAKPD
                     QSNEKRDAAQPPVTKPAAAEDLQLKKALELLRDNKSVPTQKAA"
     gene            2116..2616
                     /gene="ruvX"
                     /locus_tag="VFH91_06200"
     CDS             2116..2616
                     /gene="ruvX"
                     /locus_tag="VFH91_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET6862616.1"
                     /translation="MSKTDSSARPKGPVVALDLGSKSVGVAVSDDLLITISRLNPIKR
                     SSWKHLLINVRDLIERLDAKTLVIGLPLALDGSRTSAALDVQRLAKNFVRSLHVPVYL
                     QDERLTSVEATQTLRDAGHSDAEIRKLVDGEAAALILRDFLQSTAMEQETRLLDPNKK
                     DPKETL"
     gene            2620..3705
                     /gene="mltG"
                     /locus_tag="VFH91_06205"
     CDS             2620..3705
                     /gene="mltG"
                     /locus_tag="VFH91_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET6862617.1"
                     /translation="MKRLLLGLFALLLLSGAGIAGWAYVDLHKATTHSKSGQYIVIPK
                     GSSPSAITKRLVDDGIIRHEWPLLLYLKFSGAGSRLKAGEYDFDSPITPLQAVDKLQR
                     GQRKLDHLTIIEGWTRWDIAKAMLQIPQFKLTTEAQALELMDNVSLISDIDPAATNLE
                     GYLFPDTYEFAAETSAGDFIEMMVNRFRKVWDQDWPGHEVSLNRSPREIVTVASIIET
                     EAKLAEERPLVASVIYNRLQKNIPLAVDSSVIYASKLAGKWRYDGKVYKSDIERRSPY
                     NTRIYTGLPPGPVASPGESSLKAAMTPATSDYLYYVRNPSRNDGAHNFYNNGSEFETG
                     VQALRNWERERDAQEAQQKREANGNAP"
     gene            3806..4447
                     /locus_tag="VFH91_06210"
     CDS             3806..4447
                     /locus_tag="VFH91_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862618.1"
                     /translation="MRRLTTCLLFTLFCSFPIFGQTAKGVDKQNERVRDSGNDRTPAN
                     NGTKTDVGAGRGIDFGSGRTPETPLLANPYRLTARKDAILTAVQELMRDRKLVVDDAA
                     SKPADGIVVSQPYTFIKGAVVSEAELSRYAEVPQTNARGWTRGRYTLTVEIQPIDGTS
                     ANVAVNAKVEGRTDGATGAEWTTLRSSGLAEDQFLEALVSAVTGAAPPGRNPK"
     gene            4532..5146
                     /locus_tag="VFH91_06215"
     CDS             4532..5146
                     /locus_tag="VFH91_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862619.1"
                     /translation="MINDPFNPDATIMRERESNKRKILAVVLAFVATAAMLLGYAYMR
                     KRHAEKLIASSTVPATPDTGPKGPAVAHILIDDPMLDKGVTTIGGVVKNISKRELSGL
                     SVALELRRRKDGKTEQATVPVEPSKLIPDQAGAYSLKLPAQTYGSIKMIGLKADPEAT
                     LIAYSSSPGKKRPPERLEPKTIILGKRPVKDGEFINTFENPSRP"
     gene            5260..5844
                     /locus_tag="VFH91_06220"
     CDS             5260..5844
                     /locus_tag="VFH91_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862620.1"
                     /translation="MKQMVKGKYLSLPLGLLLSCFASQAMFAQHTSPRPTPTPTVPDA
                     ADKSAISEDPRPLTSFEEELRAKRAIKIAEKEHEENLNRAREISEIAKQLQTTFKNKS
                     ILERDDNKRLDRLEKLTKKIRGEAGGESEEVEIVNRPTDTQKAMTQIAEKAEALSDNV
                     KKTPRQVVSASVIGSANVLLELIRIARSFNLTPK"
     gene            5841..6767
                     /locus_tag="VFH91_06225"
     CDS             5841..6767
                     /locus_tag="VFH91_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6862621.1"
                     /translation="MIPKQPNFRQLFNSVAILFFFVCFLQVAKAQDDDVVRVSTNLVV
                     LNVTVTDKNGGYMKGLKLKDFKIFEDGKEVPASTISSFSFQESPYAAVVLLDSSGSME
                     TRFSLARSAAIRFLDGLRDEDVAAVYRFDSKVERVQQFSGGRDLAPMAYTIRAKGMTT
                     LNDAIVEASNVLTTRTEARKAIVVLSDGMDTSSKASSNKAVESALSVGASIYAVDMSA
                     LETPGVANRQSAASLKGFAEKTGGRFVATPGGPALRDAFTSITEELGQQYTISYRPEN
                     QTRDGKWRALEVRVARDDATVRTRKGYRLGKN"
     gene            complement(6880..>7309)
                     /locus_tag="VFH91_06230"
     CDS             complement(6880..>7309)
                     /locus_tag="VFH91_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HET6862622.1"
                     /translation="REIECAILGNDEPQASLPGEYVIYDEAARFLDYTEKYASTGNVS
                     FVVPAPLPKVLTNRIRHMAIKAFQSVDGSGLARVDFFLTRKEGDLVINELNTMPGLTE
                     VSGYPKMWAASGLPFPQLLEKLIELALERHQEKSLTRTNR"
BASE COUNT         1892 a         1852 c         1817 g         1748 t
ORIGIN      
        1 cgggagacaa cccttgtcgg agcactggct cgcgaggtgg tcgtcccagt ttgtgaagca
       61 gcactaaaac cgtccgcaaa gccggtctta taggctgttt gttctgctgg ggtgttaaaa
      121 gaacctggaa cactttcgcc ggtctgatat tccgccggta atgtgtttgt tggagtcgtg
      181 ttctgagcca cagtttcagc cgttgcaggc tggctcttat acatcacgat ggctccaact
      241 gatcctccga tcaatgctgc gattaaagct actggtaata gtttgttagt caaaatcatc
      301 gccccgtctc cttaggtata ggtctaagtg ctttttcagc cacatatcaa ttgagcaaga
      361 ggtgttccca catccaggtg gcactcatac atttcgttga ttgtaaggaa attcctgtag
      421 atttggcggg attagggggt aatgcaataa actcgcaagc gttctccaac attcgagatc
      481 gagtcgttaa tctttttttt acccgatatg aggagtgtga cattcgatat cggtggaggg
      541 aacatgaatt cagggagatc tgttctgaaa atgcacaact ggaagagact ttaaaccgca
      601 taaaaaagcc aggagagtgt cctctcctgg cttatctggc aaaactaaaa ctcgctttag
      661 gctgcttttt gagttgggac tgatttgtta tccctcagca actccagggc tttcttcaac
      721 tggagatcct ccgccgcggc tggcttagtc actggaggct gagcagcgtc acgcttttca
      781 tttgactggt caggctttgc aacgccgtcg tcatcatttc cggtcaggtc gtcaggatcc
      841 acgctatccg cgatctcggg gcctttaact tcaaccgaag gacttacgcc cgaatgattc
      901 cgatcttcac cgagaaatgc cttcccgttt cctgatgccc atttcacggt cgtgagaagc
      961 aggccatcgc cacctctcaa agtgaatagt tgctgttccg cacctgcgcc gaaactcttc
     1021 tcgccaacca cctgaccgcg attatgttcc agcagggccg aggcaataac ctcagatgcg
     1081 ccggcagtac cagtatcaat catcgcaacg accgggccgt taaaaataac tgctttcggg
     1141 tcagccgtga atgtttttaa ggcttttcct tcacgaccca ccgtttgcgc caacacgccg
     1201 tctttaataa agaaatttgc gacggcaaca gcctcgtcca gcgaaccacc tgcggtatcg
     1261 cgcaggtcga ggacaatctt ctgagcgcct tgttttacta gatcctgaag ttttgcctta
     1321 gtgtcgttag cttcgccatc acctaagctg ttgagccgca aaatccctac gcgtccggcg
     1381 tccattcgag cttctgtcgc cggcgctcga tttgttcctc tctttactgt caaggtgagc
     1441 gggttggaat tagcacgtaa gactcttaac ttcacttctg tgcctgcggc gccgttcagg
     1501 agctgctttg cgtcatacaa cgagatgtca cgagtcgctt tgccgtcaat gtactcaatg
     1561 atatcgcccg cgcgaatgcc tgcctgctcg gccgcggacc ccttcagcgg ggcaatcaca
     1621 tacagatacg acgctacctg cgacaattcc gctccaatgc cggatggagc atccttgctg
     1681 gtatttccgt tatactcacg aacttgctct ggagtcagat aggtggaata cggatcaagt
     1741 ccgtacgcca ggccccttaa ggccccggct cgaaccttct ccatatttgg ctcatctaca
     1801 taatcacttt gtatgtgttg cagtacgctc tcgaaaatac ggagctgtgc attgggatcg
     1861 ttcgctggtt gctgagcccg agttgagatc cagcctccaa caaccgtgta tatggtcaat
     1921 gtcgccgaag ccaggatcag aatgaattta gtgcgaagtg acatttactt acctctgaac
     1981 tacgttaagg acgatgccgt ttaagctcgc aaaatgcgaa acgaaacgta tcatacagtc
     2041 ggcatttatc gggcgcaagg ctaagctttg cgagtgttac gcacaattgg aaacaatgtt
     2101 tcaaccgcag attcaatgag taaaaccgat tcttcagcca ggccgaaagg tcccgtagtc
     2161 gcccttgacc ttggatctaa atcggttggg gtcgctgttt ccgacgactt attgatcaca
     2221 ataagtcggc taaaccccat aaaacgctcc agttggaaac atctcttgat taatgtgcgc
     2281 gacctaatcg aacggttgga cgccaaaaca ctggtcattg gtttgccgtt ggcgctggat
     2341 ggcagtagaa ctagtgccgc tttagacgtt cagcgtctcg ctaaaaattt tgttcgttcc
     2401 cttcacgtgc ccgtctatct tcaagacgag cgacttacct cggtcgaagc tacgcagacg
     2461 ctccgcgacg ctggtcattc tgatgctgag attcgtaagc ttgtggatgg cgaagctgct
     2521 gcactgatcc tgcgtgattt tcttcagtca acagcgatgg agcaagaaac ccgactttta
     2581 gatccaaata aaaaagatcc caaggaaact ttatagagaa tgaaacgact cctgctcggt
     2641 ctgttcgcac tcctgctgct atcgggtgcc ggaatcgctg gatgggccta tgtcgacctg
     2701 cacaaagcca ccacccactc gaaatccgga caatacatcg tgatcccaaa aggctcatct
     2761 ccttcggcta taactaagag actggtagac gacggaatca ttcggcacga gtggccactg
     2821 ctgctttatc tcaagttctc gggggcaggc tcgcgcctga aagccggcga gtacgacttc
     2881 gattctccca tcacgccgtt acaggcggtg gacaaactcc aacgcggaca acggaagctt
     2941 gaccatctga caatcattga gggttggacc cgttgggata tagctaaagc gatgcttcaa
     3001 attccgcagt tcaagttaac cacggaagct caagcattag agttaatgga taacgtgagc
     3061 ctaatcagtg acatcgatcc ggcggcgaca aacctcgagg gctatctttt cccagatacg
     3121 tacgaattcg cggctgaaac ctccgccggt gatttcattg aaatgatggt caatcgattt
     3181 cgaaaagttt gggatcagga ttggccaggc cacgaggtta gtttgaaccg ttcgccccgc
     3241 gagattgtca ctgtggcttc aatcattgag acagaagcca aattggcgga agagcgcccg
     3301 ctggtagcat cagtcatcta taaccgttta caaaaaaata ttcctttagc ggttgattcc
     3361 agcgttattt atgcatcgaa gcttgcgggc aagtggcgct acgacggtaa ggtctacaag
     3421 agtgatatcg agcgccgttc tccatataac actcgaatct acacgggatt acctccaggg
     3481 cctgtcgcgt cgccaggaga gagctcttta aaggcagcga tgaccccggc tacgtccgac
     3541 tatctgtatt acgtgcggaa tccgtcgcgg aacgatgggg cgcacaactt ctataacaat
     3601 ggctcggagt ttgaaaccgg agtacaggcg ctgagaaatt gggaacgcga acgagatgct
     3661 caagaagcac agcaaaagcg tgaagctaac ggcaacgcgc cataaattga tgactattag
     3721 gtgcttgaat ctcggagaac attaaactat tctccagttt gaatagatac ttgaactgct
     3781 tcgaacggtc aatcaaggtg caatcatgcg acgactaaca acttgtcttc tttttacatt
     3841 gttctgttct ttccctattt tcggacaaac cgcaaaagga gttgataagc agaacgaaag
     3901 agtccgcgac agcggcaatg accgtacacc agcgaacaac gggacgaaga ctgacgttgg
     3961 tgcaggacgc ggaattgact ttggtagtgg acgcacccct gagacacctt tgcttgccaa
     4021 cccttatcga ctgacggcaa ggaaagatgc gatcctgact gcagtgcaag aattgatgcg
     4081 agatcgcaaa ttggtagttg acgatgcggc gtcgaaacct gctgatggaa tagttgtcag
     4141 ccagccctac accttcatta aaggggctgt tgtttctgaa gccgagttga gccgttacgc
     4201 cgaagtcccg caaacaaatg cccgcggttg gacgcgtgga cgctacactc tcaccgtcga
     4261 gatacaacca atcgacggaa catcggcaaa cgtcgccgtt aacgcaaagg tcgaaggcag
     4321 aacggatggc gcaacaggcg cggaatggac cacgctaagg agttcaggtc ttgctgaaga
     4381 tcaatttctg gaggcgctgg ttagtgcagt cacaggtgca gctcctccgg gcaggaaccc
     4441 aaagtagaaa gagaagtaga gctcaagttc acgtaataca ttcacctgcg cgtctctctc
     4501 cgcgcgcact ctgaaagact taaagtaatt catgattaat gacccattta atcctgacgc
     4561 taccatcatg cgggagcgag aatccaataa acgtaaaata ctcgcagttg ttcttgcgtt
     4621 cgtcgcgaca gccgcaatgc tgcttggcta cgcttacatg cggaagcgcc acgccgaaaa
     4681 actaatcgca agttcaactg tcccggcaac tcccgatact ggaccaaaag gaccagcggt
     4741 tgctcatatc ctgattgacg atccaatgct cgacaaaggc gttacgacca taggcggagt
     4801 tgttaagaac atttctaaac gcgaactatc gggtctttca gtagcactgg agcttcgtcg
     4861 tcgaaaggac gggaaaacag aacaagcaac cgttccggta gagccttcca aactgattcc
     4921 cgaccaggca ggtgcctact ctcttaagct gcccgctcaa acctatggat cgataaagat
     4981 gatcggactt aaggcggatc ctgaagcaac cttgatcgcc tacagctctt caccgggcaa
     5041 gaagcgacct ccagaaagac ttgaacctaa gacgatcatc ctgggcaagc ggcctgttaa
     5101 ggatggagag tttatcaaca cgttcgagaa tccatctcgt ccatagttac ggtttgaagt
     5161 tttagtttag gagtcggcgt gccgagagtt aatgttgaat ctaagtttcc acctttggat
     5221 ttatgatttg aaaactcgcg gacgcaatgt cagttgagca tgaaacagat ggtaaaggga
     5281 aaatatttat cgctccccct cggtttactt ttgagctgct ttgcatcgca agcaatgttc
     5341 gcgcagcaca cgagccccag accaactcca actccaacag ttccagatgc cgcggataaa
     5401 agcgcaatct cagaagaccc gcgaccactg acaagctttg aagaggaact tagagcaaaa
     5461 cgagcaatta aaatagctga gaaggaacac gaagagaatc ttaatcgggc gcgtgaaatc
     5521 tctgagatcg ccaagcagtt acaaacgaca ttcaaaaata aatcgattct cgagcgagac
     5581 gataacaagc gattagatcg actcgaaaaa ctaacgaaga aaattcgagg tgaggctgga
     5641 ggtgagtctg aagaagtcga aatcgttaat cggcccacag acacacaaaa ggcaatgacg
     5701 caaatcgctg agaaagctga agcgctttca gataatgtaa agaagactcc ccgtcaggtc
     5761 gtgtcagctt cagtaattgg aagcgctaac gttttacttg agctgattcg gatcgcccgt
     5821 tcgttcaacc taactcccaa atgattccaa agcagccgaa cttcaggcaa ctctttaact
     5881 ccgtcgcgat ccttttcttc ttcgtttgct tccttcaagt agccaaagca caagacgacg
     5941 atgtggttag ggtcagcact aatctcgtcg tgcttaatgt caccgtaacg gacaaaaacg
     6001 gaggctacat gaagggcctg aagttgaagg acttcaagat ttttgaagac gggaaagaag
     6061 taccggccag caccatctcc agcttttcat ttcaggagtc gccttacgca gccgtcgtac
     6121 tcctcgacag ttcgggaagt atggagacaa gattttcact cgcacgttct gcagctatca
     6181 ggtttctcga tggactacgt gacgaggacg tcgcggctgt ctacaggttc gattcaaagg
     6241 ttgagcgggt ccagcaattt tcgggtggaa gagaccttgc gccaatggct tataccattc
     6301 gggcaaaggg catgactacc ttgaacgatg ctatcgtcga agcgtccaac gtacttacta
     6361 cacgaaccga agctcgaaaa gctatcgttg tgctctctga tggaatggac acttccagta
     6421 aagcgtcatc caacaaagcc gtcgaaagtg cattgtctgt cggtgccagt atttacgcgg
     6481 ttgatatgtc agctcttgag actccaggtg tagcaaatcg ccagagcgcc gcttcgctga
     6541 aagggtttgc ggaaaaaact ggagggcgtt ttgtcgccac gcccggtggg ccggctttaa
     6601 gagacgcgtt cacaagtatc actgaagaac tgggacagca atacacaatt tcttatcgac
     6661 cggagaatca gacgcgtgat gggaagtgga gagccctgga agtgagagta gctcgcgatg
     6721 atgctaccgt aagaactcga aaagggtatc gactaggcaa gaactaactt taaaagtttt
     6781 acttgaaggg ctgtaggggc gtcctccgtg ggcgcccttc ttccattggg ccattcttaa
     6841 cattagaggg gcggccccca cggagggacg cccctacgat taacggttgg tcctggtgag
     6901 ggacttttcc tgatgtctct caagggcgag ttcaatcaac ttctcaagta gctgcggaaa
     6961 cggcagaccc gacgctgccc acatcttcgg gtaaccggaa acctcagtca gcccgggcat
     7021 agtgttcagc tcgttgataa ctaagtcccc ttcctttctc gtcagaaaga aatccactcg
     7081 agccaacccc gaaccatcta cagactggaa cgcctttatc gccatgtgcc ggatccgatt
     7141 ggtcagaact ttgggtaagg gagcaggaac tacaaaactg acgtttcctg tgcttgcgta
     7201 tttctctgta tagtcgagaa agcgtgcggc ctcatcgtaa atcacatact ctcctggcaa
     7261 acttgcttga ggctcatcgt tcccaagaat ggcacattca atttcgcga
//
LOCUS       DASSFP010000281         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1850661, whole genome shotgun sequence.
ACCESSION   DASSFP010000281 DASSFP010000000
VERSION     DASSFP010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850661"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..570)
                     /gene="rsmD"
                     /locus_tag="VFH91_06235"
     CDS             complement(4..570)
                     /gene="rsmD"
                     /locus_tag="VFH91_06235"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET6862623.1"
                     /translation="MRIIAGAYKGRTLKSLPSMQVRPTSDRLRETLFNVIAPHIMDAR
                     FLDLCAGTGAVGIEALSRGATRTTFVDRSRKVCTLIEANVALCRITEEQAEIYNSEVT
                     DFLRQAVSWKMTPWDIVFYDPPYKDDPTPVLEFFGTHQTQLLSDEGIIIVEHHHKLEL
                     PVVIGKLERTRVLKQGDSSLSFFAGVTT"
     gene            complement(574..2850)
                     /gene="recG"
                     /locus_tag="VFH91_06240"
     CDS             complement(574..2850)
                     /gene="recG"
                     /locus_tag="VFH91_06240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET6862624.1"
                     /translation="MPGAISLNNRIEDLHSFKIARLGQILSRKLAAAVAHYANKASYS
                     DATVEDLLCYLPFRYEDRSHLTTIKDLQDGLEASLELTVKLSGGYQVRNKRAVGRSRL
                     FIFEVTATDAGNTGRPVVVWWFVSGRHCYEIVKYYTDKLTRGTRFITFGRWDWDKRRG
                     TYSLRLQKPADELETLNQIDPDQDEAEGLDPEQLEENAPDPTLAAIHVGRRVPVYRKL
                     GNFNSKRLREIIHAILENLDPATIQETLPKELRQRIKLADRKTALNEIHFPPKSASMN
                     DYETARSPAHIRLIFEDFFWLTFAIGLQRGKRIKETKPQIVKIDKAVKDAVSAVLPFK
                     LTDAQRKVTVQIFNDMKSHAPMNRLLQGDVGSGKTIVALIAMVAAMQNGLQTALMAPT
                     EILAEQHARNVKRLLAKTPYRVELLTGSLRPAEKRRLQQALTAGEIHAVIGTQALIQS
                     AVSFEKLGLVVIDEQHRFGVLQRAELRGRGLNPDVLVMTATPIPRSLAMTVYGDLDVS
                     VIDELPPGRTPVQTKVFGEDQRQEVKRLISREVRAGRQVYVVYPLVEESEKMDLKDAT
                     RRFQYLRDTVFPTFKVGLLHGKMKPADKDEVMKKFVAGEIQILVSTTVIEVGVDVPNA
                     SVMIIEHAERFGLSQLHQLRGRVGRGAEQSFCVLLTSDKQTAIAQERLGIMEKSSNGF
                     VIAEKDLELRGPGELLGTKQSGVPELRVGNLVRDQRLLEKARREAEVYLNKENSPVTL
                     KLIQHVSDGSKFGLAAVG"
BASE COUNT          723 a          786 c          754 g          793 t
ORIGIN      
        1 cgatcaggtt gtaaccccgg cgaaaaagct gagtgatgaa tcaccctgtt tgagcactcg
       61 ggttcgttct aacttgccga ttactacagg caactctaat ttgtgatggt gttcgacgat
      121 aattatgccc tcgtcgctga gtagttgcgt ttgatgcgtc ccaaagaact caagaacggg
      181 ggttggatcg tccttatatg gagggtcgta aaagacaatg tcccatggtg tcatcttcca
      241 ggagacagct tgtcgaagaa aatccgtaac ctctgaattg taaatctcgg cctgctcttc
      301 tgtgatccgg cacaaagcga cgtttgcttc gatcaaggtg cagactttgc gggatcgatc
      361 cacaaaagta gttcgagttg cacctctcga caacgcctca atccccacag cgccggtccc
      421 tgcgcataga tcgagaaacc gagcgtccat aatatgcgga gcaatgacat tgaagagggt
      481 ttcacgcaga cggtctgaag tgggtcgcac ttgcatcgaa ggcagactct tgagtgtgcg
      541 acctttatag gctccagcaa tgatccgcat caattaacct actgcagcaa gtccaaactt
      601 tgatccgtca ctgacgtgct ggatcaactt gagtgttacc ggagaatttt ctttattaag
      661 gtacacttcg gcttcgcgtc gagctttctc gagcagccgc tgatccctta ctaaatttcc
      721 aactcgcaac tcaggcacgc ctgattgttt tgttcccaaa agttcgcctg gtccacgcag
      781 ctcgagatct ttctcggcga tgacgaaacc gttgctactc ttttccatga tgcccagccg
      841 ttcctgagct atggccgtct gtttgtcact ggtcaaaagt acgcagaagc tttgctctgc
      901 tccgcgacca actcgaccgc gcaattgatg tagttgtgac aagccaaatc gttctgcgtg
      961 ctcaataatc attactgagg cgttcggcac atctactcct acttcgatta cggtagttga
     1021 gacgagtatc tgaatctccc ccgcaacgaa cttcttcatc acttcgtctt tatcagccgg
     1081 cttcatcttt ccgtgaagta atcccacctt gaaggtagga aacacggtat ctcgaagata
     1141 ttgaaagcga cgagttgcat ccttgaggtc catcttctcc gactcttcca cgagaggata
     1201 gaccacatag acttgccggc cagctctaac ctctcgagag attaatctct tcacttcctg
     1261 acgctggtcc tcaccaaaaa ccttcgtttg caccggagtg cgacccggcg gaagttcatc
     1321 aatcactgaa acatccaggt cgccgtacac agtcatggcc aacgagcgcg ggatcggggt
     1381 cgcagtcata acgagaacat ctggattcaa gcctcgtcca cgcaattcgg cgcgttgcag
     1441 aaccccaaat cgatgttgtt catcaataac cactaaaccc aacttttcaa aagacacagc
     1501 actctgaatc aatgcttgcg tcccgatgac cgcgtgtatt tcaccggcgg tcaaagcttg
     1561 ttggaggcgc cgcttttcag ccggacgcaa tgaaccggtt agcaattcga cgcggtaagg
     1621 tgttttcgca aggagtctct tcacgtttct ggcgtgctgt tcagcgagta tttctgttgg
     1681 ggccatgagg gctgtctgca atccattttg catcgcagcg accattgcga taagagctac
     1741 gatcgttttt ccactaccga catcaccttg caaaagccgg ttcatcggag cgtgcgactt
     1801 catgtcgttg aaaatctgga ccgtgacttt tctttgagcg tcagtaagtt tgaaaggaag
     1861 cacagccgac acggcatcct ttaccgcttt atcgattttt acaatttgcg gtttcgtttc
     1921 cttgatccgc ttaccacgct gtagaccaat agcgaaagtt aaccagaaaa agtcttcgaa
     1981 gatcagtctg atgtgtgcgg ggctacgagc agtctcatag tcattcatcg acgccgattt
     2041 gggagggaaa tggatctcat tgagcgctgt tttgcgatcg gccagtttga ttctttgccg
     2101 caattcctta ggaagagttt cctgaatcgt tgcgggatcg agattttcaa gaatagcgtg
     2161 gatgatctct cgcagtcgtt ttgagttgaa gtttccaagc ttgcgataga cgggaactcg
     2221 acggcccaca tgaatggcag ctaaggtcgg atccggagca ttttcttcga gttgctcggg
     2281 atctaatccc tcggcttcat cttgatccgg atcgatctgg ttcagggttt cgagctcgtc
     2341 agcaggtttc tgcagcctca gtgagtatgt accgcgtcgc ttgtcccagt cccagcggcc
     2401 gaaggttata aagcgagtac ctcgcgtcaa tttgtcggtg tagtacttga cgatttcgta
     2461 gcagtgacgg ccggaaacaa accaccacac gaccactggt ctaccggtgt taccggcatc
     2521 ggtcgcagtg acctcgaata taaagagtcg tgatctccca acagcacgtt tgtttcgtac
     2581 ttgataaccg cccgatagtt tgaccgttaa ctcgagcgag gcttccagtc catcctgaag
     2641 atctttgatc gtagtcaggt gcgaccgatc ctcataacgg aaaggtaaat agcaaagcag
     2701 gtcctcgaca gtagcatcag aatagctcgc cttgttagcg taatgtgcta cagctgctgc
     2761 gagtttacgg gaaaggattt gtcctaaccg cgcaatctta aaactgtgaa ggtcctcaat
     2821 ccggttgttg agggagatgg cgccaggcat tggtgggatc atagcatagc gaaccggaac
     2881 aggtggcact ggcaggaccg ttcaatagtg tcttaatctg taggggcgtc tgcggtgtcg
     2941 atatgcttta ccttgtcggg ggaatttatc gattcgacgc cagccaagct aaaagcatat
     3001 cggacaccgc agacgcccct acagattaag acactactga acggtcctgc cagtgc
//
LOCUS       DASSFP010000282         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_974085, whole genome shotgun sequence.
ACCESSION   DASSFP010000282 DASSFP010000000
VERSION     DASSFP010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974085"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFH91_06245"
     CDS             <1..306
                     /locus_tag="VFH91_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HET6862625.1"
                     /translation="ITGEDANVKTYLGRPWRDFSSVVFLNTEMSAVVRPEGWQDWDLP
                     AREKTSRYAEFNSYGPGANIKSRVSWARQIDRGRAKKITAEKILRGADGWNPHARAN"
     gene            complement(395..1087)
                     /locus_tag="VFH91_06250"
     CDS             complement(395..1087)
                     /locus_tag="VFH91_06250"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HET6862626.1"
                     /translation="MKTLGLIGGVGPESTVDYYRQLVSGFRERAGGSYPPVIINSVDL
                     KRLLDWMAQNRLDLVADYLAEEIQKLELTGADFAALASNTPHIVFDELQRRSNLPLIS
                     IVDAACRRVQKLGMKTVGLFGTRYTMQATFYPNTFARLGLTLVVPDANEQAYIHEKYL
                     GELLNDMFLAETRSQMLSIAGRLQKEHGIEGLILGGTELPLLLRDDQYDGLPMLDTTR
                     IHVAELINEMLT"
     gene            complement(1094..3274)
                     /locus_tag="VFH91_06255"
     CDS             complement(1094..3274)
                     /locus_tag="VFH91_06255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6862627.1"
                     /translation="MRFRILLFLAVVVSLQSPTFAQKRSITEKDLFNFVWAGDPQVSP
                     DGSRIAFIRVTVNDRKDGYNTAIWLVETASGATRQLTRGTRDANPRWSPDGQYLVFVR
                     SSEREGRTDPPQLFMMSMSGGEPFQFTTLPRGAGNPQWSPDGKSILFYNGATQEEITK
                     AQRAAAAPSPSPSASPKPEERESDVRVITRAIYRSNGAGYVDNKHPTHLWVVVAPRTG
                     DEKVTPKQLTRGRFDDGNATWSKDSTQIYFVSDQTEEPYYELPNTDIYSVSATGGSVT
                     KLTTFDMGAGALSVSPDGRKFAFYASTNKPVQSYTEPDLWVLDIAPNAQPKNLTRDFN
                     FDVGSGVGGDNTAPRGGGGNPPIWSADGSKIFAIFTKEGKANLGSFDSTTGRETDVTT
                     GNQAVVTFRSVPGAAKFALLISTPTKIGDLYWLDGSSGTPKQLTHLNDELFSKLNLTE
                     PEEVWYTSFDGKKVQTWVQKPPDFDPNKKYPLILNIHGGPHTAYGFIFDHEFQWMAAK
                     GYVVLYPNPRGSTSYGQEFGNIIQYRYPGDDYKDLMAGVDELLKRGYIDEKRLGVTGG
                     SGGGLLTNWTIGQTTRFAAAVSQRDIADWADWWYSADFTLFQPSWFKAPPFEDPEDYR
                     ARSPITYINKVQTPLMLILGEADYRTPTGAGGEQMFRALKYRKIPTVMVRFPNETHEL
                     SRSGQPWHRIERLEHIVGWFDKWLMGMPKPEYEIGEVTSTAVNQ"
     gene            complement(3361..>3717)
                     /locus_tag="VFH91_06260"
     CDS             complement(3361..>3717)
                     /locus_tag="VFH91_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862628.1"
                     /translation="SKINPGGKKLDCKKSQPLNTSFAAHVAPLGLEYFDNGSSDPLRN
                     TFLVALHGSTNKRIARGYRVVQVVNGSTRDFITGFLQGGVVNGRPCDIFKLGPDSFLL
                     TDDKAGVIYLIEKKRR"
BASE COUNT          876 a          917 c          970 g          954 t
ORIGIN      
        1 atcacgggag aagatgcgaa tgtaaaaact taccttggca gaccgtggcg tgacttttcg
       61 agtgtggttt tcttgaacac agagatgtca gccgttgtca ggccagaggg ttggcaggat
      121 tgggatttac ccgcccgcga gaaaacatcg cggtatgctg agtttaacag ttatggtcca
      181 ggtgcgaata ttaaatctcg cgtctcgtgg gccaggcaga ttgatcgtgg ccgggcaaag
      241 aaaatcaccg ccgagaagat attgcggggt gcagacggat ggaatccgca cgccagggca
      301 aattaacgta acgagtttgg gtctctgcgt tcctcagcgt ctcggtggct ctgcgttgat
      361 cggaggttta ccgcagagcc gcggagacgc agagtcaagt cagcatctcg ttaattaatt
      421 cggcaacatg aattcgcgtt gtatcaagca tgggcaggcc gtcatattga tcgtctcgga
      481 gcaaaagagg aagttctgtt ccacccagaa tcagtccctc gatgccatgt tccttttgca
      541 accgtccagc gattgacaac atttgactgc gcgtttctgc cagaaacata tcgttgagga
      601 gttcgcccag atacttctca tgaatgtagg cctgctcatt tgcgtcgggc acgaccagtg
      661 tgagtcccag tcgtgcgaac gtgttgggat aaaaggtcgc ctgcatcgtg taacgagtac
      721 cgaatagacc aacggttttc attccaagtt tctgtactct tcggcaagca gcatcgacaa
      781 tacttatcaa aggaaggttg gaccgtcgct gtaattcatc aaatacgatg tgtggtgtat
      841 tcgaggcgag cgcggcgaaa tcggctcccg taagctcaag cttctgaatc tcctcagcca
      901 gataatccgc gaccagatcg agacgatttt gcgccatcca gtcgagcaag cgtttgagat
      961 caacgctatt gatgatcact ggaggataac taccgccagc tctttcacga aatcccgaaa
     1021 caagctggcg gtaataatca acggtcgatt ccggacctac gcctcctatt aagccaagag
     1081 ttttcatttc agtttactgg ttaacggcgg tgctggtgac ctcaccgatc tcgtattcag
     1141 gttttggcat ccccatcagc cacttatcaa accagcctac tatgtgttct aagcgttcga
     1201 tccgatgcca tggttgtccc gaacgcgaca actcatgcgt ctcgtttgga aaacggacca
     1261 tgacggttgg tatctttcta tacttcagag cgcgaaacat ttgctcgccg cctgcccccg
     1321 tgggcgtgcg atagtcggct tctcccagga tcagcatcaa cggcgtctgt actttgttga
     1381 tgtaagtgat aggtgaacga gcgcggtaat cctctggatc ttcaaacggt ggcgccttaa
     1441 accagcttgg ttggaaaagt gtaaagtcag ctgagtacca ccagtcggcc caatcagcaa
     1501 tgtctcgttg cgaaactgcc gccgcaaaac gagtggtttg acctatcgtc caattcgtaa
     1561 gcaagccacc gccactgcct cctgtaactc ccaatctctt ttcgtcgatg tagccacgct
     1621 tgagcagttc gtcgacgccg gccatcaggt ctttgtaatc atcgccagga taacgatact
     1681 ggatgatgtt gccgaattcc tgaccatagc ttgtgctgcc tcggggattt ggatagagaa
     1741 cgacgtaacc cttcgctgcc atccattgaa actcatggtc aaaaatgaat ccgtaggcag
     1801 tgtgtggtcc accgtggata ttcaggatca atggatattt cttattcgga tcgaagtcag
     1861 gtggtttctg gacccacgtt tgtaccttct ttccatcgaa gctcgtgtac cagacttctt
     1921 cgggctcggt caaattaagt tttgaaaaga gctcatcgtt cagatgcgta agttgcttcg
     1981 gagtgccgga gctaccatcg agccaataca aatcgccaat ctttgtgggt gtagagatga
     2041 gcagcgcgaa ttttgctgcg cccggaaccg accgaaaagt tacgactgct tgattgccgg
     2101 tcgttacatc agtctcccgg ccggtggtgg aatcgaatga accgagattg gcttttcctt
     2161 cctttgtaaa aatagcaaaa atcttgctgc cgtcagctga ccaaattggt ggattgcctc
     2221 caccaccgcg aggggcagta ttatcaccgc cgactccgga tccaacgtcg aaattaaaat
     2281 cacgggttaa gttctttggt tgagcgtttg gggcaatatc caggacccag agatccggct
     2341 ccgtatacga ctgaacaggt ttgttggttg aagcgtaaaa agcaaacttc cttccgtcgg
     2401 gactgaccga taaagctccg gcacccatgt cgaaggtagt tagttttgta accgagccac
     2461 cggttgccga gaccgaataa atatcggtat ttggcagttc gtaatacggt tcttcggtct
     2521 ggtcggaaac gaaataaatt tgagtgctgt ccttggacca tgtggcgtta ccgtcgtcaa
     2581 aacggccgcg ggtaagttgt ttcggggtaa ctttttcgtc gcctgtacgg ggcgcgacga
     2641 caacccatag atgcgttgga tgtttgttat caacatatcc tgcgccgttc gagcggtaga
     2701 ttgcgcgcgt aatcactctg acatcagatt cccgttcctc gggtttggga ctagcacttg
     2761 gagaagggga tggtgcagca gctgcgcgtt gagcctttgt gatctcttcc tgggttgcgc
     2821 cgttataaaa caagatcgac ttaccatccg gcgaccattg cgggttccct gctccacgcg
     2881 gcaatgtcgt gaactgaaag ggctcacccc cgctcattga catcataaag agttgcggag
     2941 gatctgtgcg gccctctcgt tccgacgacc gaacgaaaac aaggtattgt ccgtcaggcg
     3001 accaacgagg gtttgcgtct cgtgtacctc gggtgagttg gcgtgttgct ccactcgctg
     3061 tttcaaccag ccagattgct gtgttgtaac catcttttcg gtcgttaacc gttacccgga
     3121 tgaaggcgat ccgtgaacca tccggagaaa cctgcggatc accggcccac acaaaattga
     3181 acagatcctt ttcagtgatg cttcgttttt gagcgaaagt tggtgactgc agcgagacta
     3241 cgacagcgag gaagagcagg attcggaacc tcattcattt ttcctttcag cggacggttg
     3301 gcgaattagg cggactattt tactccatct aagtgatatt tatcattgga taattatcgc
     3361 ttacctgcgc ttcttctcaa tgagataaat aacgcctgct ttgtcgtcag tcagaagaaa
     3421 gctgtctgga ccaagtttga agatgtcgca cggccttcca ttaacaactc cgccctgtag
     3481 aaagccggtg atgaagtcgc gagttgaacc attcacaact tgtacgaccc ggtaaccacg
     3541 ggcaattcgt ttgttggtag atccatgcag cgcgaccaaa aatgtattgc gaaggggatc
     3601 ggagctaccg ttgtcgaaat actccaggcc gagtggtgca acgtgtgcgg caaatgaagt
     3661 gttaagaggt tgtgactttt tacagtcgag cttcttcccg ccgggattaa tttttga
//
LOCUS       DASSFP010000283         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_519439, whole genome shotgun sequence.
ACCESSION   DASSFP010000283 DASSFP010000000
VERSION     DASSFP010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519439"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="VFH91_06265"
     CDS             complement(<1..2067)
                     /locus_tag="VFH91_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6862629.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGAPAIEPEHLLLGLMREDKTL
                     TGRFFPRAQVSIESIRKEIEGRTLLREKISTSVELPLAPETKHVLAFAHEESDRLQHR
                     HIGTEHLLLGLLREERSMAAEILYERGLRLNAVRDEIARQSGSDARASQKKDTPHLVE
                     FSRDLTDDAGNDKLDPLIGREAEIERLVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIVRGDVPSFLENKRILSLDLSLIVAGTKYRGQFEERLKQIMRELVENPQYIVFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEFRKSIEKDRSLERRFQAVKV
                     PPPSEEEAVSILDGVRERYESFHQVRYTDDALQAAVYQSHRYIPDRFLPDKAIDVIDE
                     AGARVKLRVRREQGNLADWNELNEWSRSYGDTSTLRSSQEDALVSAQVTRDDVEDVIA
                     RWTGIPVTSLKEEETQKLLRIEVELHKRVVSQRPAISALARAIRRSRAGLKNPLRPVG
                     SFLFLGPTGVGKTEVARSLAQFLFSSERALIRFDMSEFMEKHSVSKLIGSPPGYVGHE
                     EGGQLTERIKRSPYSVLLFDEIEKAHPDIFNILLQVFEDGILTDALGNTVDFKNAIII
                     MTSNIGARFIQKKGTLGFQANTNASREKMEEMVMSSVRQTFNPEFINRLDEIIIFDQL
                     LDVELLE"
     gene            complement(2226..2954)
                     /locus_tag="VFH91_06270"
     CDS             complement(2226..2954)
                     /locus_tag="VFH91_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862630.1"
                     /translation="MRIADQTIETFSPVSSVGEGLNVVDLRKTYLTTTGARLEVLTGL
                     TLKAEPGEAVAIRGASGSGKSTLLHLLGGLDEADHGMIDLGGTELFKLSSRSLESFRQ
                     NKVGFIFQFHYLLQDLSAIENVVLPLMISRRKRVEAVKLARELLQKFGLGDRLESRVG
                     QLSGGEQQRVAVARALITKPKLLLADEPTGNLDSSIGAEIGDLLVEYAHRSSSIVIVA
                     THNEDLVRVCDRGLILKDGRLHPL"
BASE COUNT          767 a          831 c          738 g          769 t
ORIGIN      
        1 ctcaagaagt tccacgtcga gcaattgatc gaaaatgatg atctcgtcga gccgattgat
       61 gaactccgga ttgaacgttt gtcgcaccga cgacattacc atctcttcca tcttttcgcg
      121 tgaagcattg gtatttgcct gaaaaccgag cgtacctttc ttctgaatga aacgggcgcc
      181 aatgttcgac gtcatgatga tgatggcatt cttgaaatcg accgtgttgc ccagcgcgtc
      241 ggtaagaatt ccatcttcaa atacttgcaa caggatattg aaaatgtctg gatgcgcttt
      301 ttctatttca tcgaagagca aaactgaata tggcgaccgt ttgatgcgtt ccgtcaactg
      361 gccaccttct tcgtgaccaa cgtaacccgg aggagatccg attaacttcg agacggagtg
      421 cttttccata aactccgaca tatcgaaccg gatcaacgca cgctcactgc taaacaagaa
      481 ctgcgcgagc gaccgggcca cttcggtttt ccctactccg gttggaccaa ggaacaggaa
      541 cgagccaact ggtcggagtg ggtttttcaa tccagctcga gatcgacgga tcgcacgggc
      601 caatgcggag atggcaggac gctgagacac gacgcgctta tgcagttcaa cttcgatccg
      661 caaaagcttt tgagtctctt cctcttttag cgaagtcact ggaatgcccg tccaacgcgc
      721 aatcacatct tcgacatcgt cacgtgtgac ctgagctgaa accagggcat cttcctgact
      781 ggaccgcaaa gtcgaagtat cgccatacga tcgcgaccat tcgttcaatt cattccaatc
      841 ggcgagattg ccttgctcgc gtctcactcg cagctttaca cgcgcaccgg cctcgtcaat
      901 cacatcaatg gccttatcgg gtagaaagcg gtctgggatg taccggtgcg actgatagac
      961 agccgcttgc aaagcatcgt ccgtatatcg aacctgatgg aaactttcgt aacgttcacg
     1021 cacgccatcg aggatactta ccgcctcttc ctcagaaggt ggcggaactt ttaccgcctg
     1081 gaagcggcgc tcaagcgatc ggtccttctc aatcgacttt cgaaactcgg cgggagtcgt
     1141 cgcgccgata cattgaattt ctcctcgaga aagagctggt ttcagaatgt tagccgcatc
     1201 gagcgatcct tcagcagagc cggcgccgac cagagtatgg agttcatcga tgaacacaat
     1261 gtactgaggg ttctcgacta actctctcat gatctgtttg agacgttctt caaactggcc
     1321 cctgtatttt gtgcccgcca caatcagcga gagatccaga gacaatattc tcttgttctc
     1381 gagaaatgat ggcacatcgc cgcgaacaat tcgttgggcc agaccttcaa cgatggctgt
     1441 cttaccaaca cctggctcac caattagcac gggattgttc ttagtgcggc gacaaagaat
     1501 ctgtacgagt ctctcaatct cagcttcacg accaatcaaa ggatcgagct tgtcattgcc
     1561 cgcatcatca gtgaggtcgc gcgaaaactc gaccaggtgt ggagtgtctt tcttttgtga
     1621 agcgcgtgca tccgaaccag attgtctggc gatctcgtca cgcactgcgt tcagacgcaa
     1681 tccacgttcg taaagaatct cggccgccat cgagcgctct tcacgaagca aaccgagcaa
     1741 caagtgttca gttcctatgt ggcgatgttg taaacggtcg ctttcctcat gcgcgaacgc
     1801 gaggacatgc ttcgtttcgg gcgccagcgg caactcgact gaagtagaga ttttttctcg
     1861 caggagagta cgaccctcga tctctttgcg gatcgattcg atcgaaacct gtgcacgggg
     1921 gaaaaatcgg ccggttaaag tcttgtcctc ccgcatgaga ccaagcagta ggtgttccgg
     1981 ctcaatcgca ggagcaccaa actgtgaagc ttcataacga gcaaaaaaga ttacgcggcg
     2041 ggccttttct gtataacgtt cgaacatcgt gtttttctac taacaggtcc ttatgattgc
     2101 catgccgggc caaaactgag cgcccggcac ttgtctggca ttataaccta tgagtgaagg
     2161 tgcaaaaagc attgtcttct ttttagataa acacttaaca atgttttctt ttcagccgtc
     2221 ccgttttaaa gtggatgcag acgaccgtcc tttaagatca aaccacgatc acagaccctg
     2281 acgaggtctt cattgtgtgt cgctactatc acgatcgatg agcttctatg agcatattcg
     2341 accaatagat cgcctatttc tgctccaatt gaagaatcaa ggtttccggt gggttcatct
     2401 gccagtagca actttggttt cgttattagg gccctggcaa cggcaactcg ttgctgctcg
     2461 ccacccgaaa gctgaccaac tcgagactca agtcgatccc ccaatccgaa cttctggagt
     2521 agttccctgg caagtttgac cgcctcaacc ctctttcgac gcgagatcat cagcggtaac
     2581 accacatttt cgattgctga aagatcctga agcagatagt gaaactgaaa aataaaacct
     2641 accttgtttt gtcgaaacga ctcgagagac cggctcgata acttaaatag ctcagttccg
     2701 cccagatcga tcatgccgtg atccgcctca tccaaaccgc cgagtagatg aagaagggtt
     2761 gatttccccg aacctgacgc tccccggatt gcaacggctt caccgggttc agctttcagt
     2821 gttagacctg tcaacacctc aagacgcgct ccagttgttg tcaagtaggt tttcctcaag
     2881 tcgaccacgt tcagcccttc acctactgaa gagacaggac tgaatgtttc gatcgtctgg
     2941 tcggcaatcc gcatatcaga tggaatccct gagtacttcg acaggtcttg tttgtgaggc
     3001 cgctcgagcg ggatagatag tggcaactac gctcagcact atagctacaa gggccgataa
     3061 aaatgcctca ctcaaatgga cgtttagcgg gacactgctg attga
//
LOCUS       DASSFP010000284         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1408817, whole genome shotgun sequence.
ACCESSION   DASSFP010000284 DASSFP010000000
VERSION     DASSFP010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408817"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFH91_06275"
     CDS             complement(<1..599)
                     /locus_tag="VFH91_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HET6862631.1"
                     /translation="MSQLADQVQGRNDLKPDLDGSVGILGVPLGFGASMAGVDMGPAA
                     LRVARLHQRIAALGYSVTDFGDLHPERPQTLPATDDKLKYLAEISKACEQLAIKVEEI
                     LESGRLPVVLGGDHSIAIGSFSGVASFYRKRQETIGLIWFDAHADMNTPETTPSGNIH
                     GMPLAALLGFGTSELTNVAGFSPKIDPQFCAHVGARDIDA"
     gene            complement(603..1154)
                     /locus_tag="VFH91_06280"
     CDS             complement(603..1154)
                     /locus_tag="VFH91_06280"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HET6862632.1"
                     /translation="MIEAAERIVTIFGGSKCHESDPEYIQAEQVGSLLAQAGFTICTG
                     GYLGVMEAASRGAREAGGRVLGIVMNQFKTEPNRFLTDKVATPHFYERLQRLITRSVG
                     FVALRGGMGTVTELSLVWNKIQTRVIEPRPLVLLGECWRPVVKQWQDCLAVSDSDVAA
                     LDFALTPDEAVEIIKKKSRGVVL"
     gene            complement(1151..1852)
                     /locus_tag="VFH91_06285"
     CDS             complement(1151..1852)
                     /locus_tag="VFH91_06285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6862633.1"
                     /translation="MESAAVKKDEHLRLEFNEWARDGRGESMEKGHRPIGVQAIEQMN
                     IPATASVLDVGCGSGWATRFMADKATQGQVTGIDISNEMIEVARAQSAAYQNVRFDLA
                     SAESLPFKDGEFTHVFSMESLYYYSNMLAALREIKRVLRSGGLFVTVIDLYLENSPSH
                     QWIPTLKVPVHLLGVPDYRSMLEQLGFVNIKDQRLHDPAPVPEDYSGGSFKTREDYLL
                     YKQNGSLMLSAGVER"
     gene            complement(1958..>2206)
                     /locus_tag="VFH91_06290"
     CDS             complement(1958..>2206)
                     /locus_tag="VFH91_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET6862634.1"
                     /translation="EGYGEVIGGGERATSLEFLREQIALHELPEEAFQWYLDLRRYGT
                     VPHAGFGMGIERCTAWMCGIEHIRETIPFPRMLYRIRP"
BASE COUNT          556 a          597 c          509 g          544 t
ORIGIN      
        1 gcgtcgatat cgcgagctcc aacatgagcg cagaactgtg ggtcgatctt tggtgagaat
       61 cccgccacat ttgttagttc cgaagtaccg aaaccaagaa gtgcagctag tggcattccg
      121 tgaatattgc cggaaggagt agtctcgggt gtattcatat cagcatgtgc gtcgaaccag
      181 atcaaaccaa ttgtttcttg ccgctttcga taaaacgagg cgacgccaga aaatgaaccg
      241 attgcaatcg aatgatctcc tcccaatacg acgggaagcc gccctgactc aaggatctcc
      301 tcaaccttga tcgctaattg ttcgcaggct ttgcttattt cggccaggta cttcagctta
      361 tcgtcggtcg ctggtagggt ttgcggtcgt tcgggatgaa gatctccgaa gtccgtaact
      421 gaatatccaa gcgcagcgat acgttgatgt aacctcgcta cgcggagcgc ggctggtccc
      481 atatcgacac cagccatgct cgctccaaag ccgagtggaa caccgaggat accgacactg
      541 ccatcgaggt ctggcttaag atcgttcctg ccctgaactt gatcagcgag ttgagacatt
      601 agctataaaa caaccccacg agactttttc tttatgatct cgacggcttc atcgggcgtc
      661 aaggcaaaat caagagctgc gacatctgaa tcactcaccg caagacaatc ctgccattgc
      721 ttaactacag gacgccaaca ctcgccgaga agtaccaaag gtcgcggctc aatcactctc
      781 gtttgaattt tattccacac caacgataac tcagtaacag ttcccatacc gccacgaaga
      841 gcaacaaatc ccacagatct ggtgattagt cgctggagac gttcgtagaa gtgtggtgta
      901 gcaactttgt cagtcaggaa cctgtttggt tcagtcttga actgattcat cacaattcca
      961 agaacgcgtc cgccagcttc acgtgcgcca cgacttgctg cttccattac gccgaggtag
     1021 cctccggtac agatcgtaaa gccggcctgg gccaacaggg atccaacttg ttccgcctgg
     1081 atgtattcag gatccgattc gtgacacttt gatcctccaa aaatggtcac aatcctttcc
     1141 gcggcttcga tcatctttct actccggcac taagcatgag agagccattc tgtttataga
     1201 gcaagtaatc ctcgcgcgtt ttaaatgagc ctccagaata atcctcaggc actggggcgg
     1261 gatcgtgcag tcgttgatct ttaatgttta cgaagccgag ttgctcgagc atcgaacggt
     1321 aatcaggaac gcctaacaaa tgcacgggca ctttaagcgt tgggatccat tgatgcgacg
     1381 ggctattttc cagatacaaa tcaatgacag tgacaaagag tcctccgctt ctgagaacac
     1441 gttttatctc tcttaacgcc gcaagcatgt tggagtagta atagagagat tccatcgaga
     1501 acacatgagt gaattcgcca tccttgaacg gcagcgactc tgcacttgca aggtcgaatc
     1561 gcacgttttg atatgcagcc gattgggcac gcgcgacttc aatcatttcg ttcgagatat
     1621 ctattccagt cacttgaccc tgggttgcct tatcggccat gaagcgagtg gcccatccag
     1681 aaccacaacc tacatcaagc acgctggctg ttgccggaat attcatctgc tcgatcgcct
     1741 gaactcctat tggccggtga cccttttcca tgctttcacc acgaccatca cgagcccatt
     1801 cattaaactc caaccttaag tgttcatcct ttttgacggc agcgctttcc ataagaatat
     1861 agtctccaga aacagatgtg cgtggcaaat gcgtctctcc tgagaacaca cttacacttt
     1921 ttaacttgat gtggaaaagc ttacgcgatt agacagatta cgggcgaata cgataaagca
     1981 tgcgggggaa cggtatcgtt tcgcgaatat gctcgatgcc acacatccat gcggtacatc
     2041 gttcgattcc cattccgaag cctgcgtgcg gcaccgtgcc atagcgtctc agatccaggt
     2101 accactggaa cgcttcttcg ggtagttcat gcagcgcaat ctgttcacgc aaaaactcga
     2161 gtgacgttgc acgctcaccg cctccgatta cttcaccgta accttc
//
LOCUS       DASSFP010000285         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1111171, whole genome shotgun sequence.
ACCESSION   DASSFP010000285 DASSFP010000000
VERSION     DASSFP010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111171"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="VFH91_06295"
     CDS             <1..1857
                     /locus_tag="VFH91_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET6862635.1"
                     /translation="NSWQFNAGITPGGSGGYMLQRQQAAYLTSGTRPAANDSRIQNAV
                     FRNGSLWATHTVLLSTTPLVAGTPTSVANPHNHAGIQWWEIDPTIVNSASGTLPIQRG
                     RIQDPTADNCNNNSGGSRGGCTPQGQFFAFPQISVNQHDDVLIGYSRFSERTYASAAY
                     SFRAGTDPANTMRDSIVFRPGQSNYNIGGGAPFTVRWGDMSHAQVDPLNDTDFWTTQE
                     YADVNRLTGIGIAGTWSTWWARVSPSTPAPSTTGTLIINEFRLRGPQGPRDEFVELYN
                     PGTSPLVVTTSDGSDGWALAYSNDGTAVTAVTTIPVGTVIPAKGHFLITSNQVSPITA
                     TAVYSLANASNTAFRDSGGDTGYAIDNPDNGGFAIFKTSIPANFAIGTRMDSVGFSGI
                     AAGLFREGAGLPPISTTSPTGQASHVRFIGRLGEPHDTGANEADFIYVNPVNELSPTP
                     TQGAAGPENLDSPIIQSNVVDALVAPCIAATLPPNRVRVGSGNGGTLSIRRTLQNNTG
                     AAVTRLRFRVVEITTAPAPDLATAILAVTSSSNSIEASPCGPNVTITGLTLEDTPVPV
                     QPQGGGQNSTLSAGTITLAAPLANGSSIRVNFLTNVVQAGHFTFFVNVEALP"
     gene            complement(2035..2517)
                     /locus_tag="VFH91_06300"
     CDS             complement(2035..2517)
                     /locus_tag="VFH91_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HET6862636.1"
                     /translation="MPDAKLQAHKSWRLALALARKTVRHPKELFLMFRMASWIGVVTL
                     VARLWSLPKALEFVSGKDIAGSRDRADLDKRLANTLDQLLAADVVFLKPKCWKRAAVL
                     RRYLSKNGIPTTIVFGVRRDTQGNLDGHAWLEFEGEPILESSPPEYVVTYRYPETVAL
                     "
     gene            2647..3540
                     /locus_tag="VFH91_06305"
     CDS             2647..3540
                     /locus_tag="VFH91_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862637.1"
                     /translation="MTEPSRILLQTTIPDVEDDWHVGRFSLLRQHLSSLKDDQGNSLF
                     VVAARNRQTNADGNDEVLSQLDKSDFDQLWIFAVDTGNGLTIADCEGITRFRQRGGGI
                     LSMRDHQDLGSSLCTIGGIGRAHYFHSKQQEPDESRRSRDDQATAQITWPNYHSGNNG
                     DYQLITTVEPVHELLRRGDGEVIKYFPAHPHEGAVGVPADESNARVIATGESQTTHRP
                     FNLVVAFEKASDSHGNELGRAIAESSFHHFVDYNWDISMGCPSFLVETPGHQIQNEPE
                     KLSDIKTYVRNLALWLTPQRN"
BASE COUNT          952 a         1028 c          952 g          872 t
ORIGIN      
        1 aattcatggc agtttaacgc gggcatcact cccggcgggt ctggtggcta catgcttcag
       61 cggcaacaag cggcatatct gacttcaggc acgcgcccag cggcaaatga ctccagaata
      121 caaaacgctg ttttcagaaa tggatctcta tgggctaccc acacggtttt gctctcaaca
      181 acgccgctcg tggctggaac tcccacttcg gtcgcaaacc cacataatca cgccggcatt
      241 cagtggtggg agattgatcc aaccatcgtg aatagcgcat caggaaccct tccgattcaa
      301 cgaggccgca ttcaggatcc gactgctgac aattgtaata acaattccgg cgggtcccgg
      361 ggtggatgca ccccgcaggg ccagttcttc gcctttccac aaatcagtgt caaccaacat
      421 gacgacgtgt tgattggata ttcgcgtttc tctgaacgta cttatgccag cgcagcatac
      481 tcgtttagag ccggcacgga ccctgcaaat acgatgagag actcgatcgt cttcaggcct
      541 gggcaatcta actacaatat tggtggtggc gcaccgttca ctgtccggtg gggtgacatg
      601 agtcatgctc aggttgatcc tttaaacgat actgactttt ggaccacaca ggaatatgct
      661 gatgtgaatc gtctaaccgg catcggcata gctggaacat ggtctacctg gtgggcgcgg
      721 gtatcgccca gtacgcctgc cccctctacg acgggaactc tgataatcaa tgagtttcgt
      781 ctgcgtggtc cacaaggccc gcgtgacgag ttcgttgaac tatataaccc gggcacgagc
      841 ccactggttg tgaccacgtc agatggatca gatggttggg cgctcgctta cagcaacgat
      901 ggaactgccg tcacggctgt aactacaatt cccgtaggaa ctgtaattcc cgcaaaagga
      961 catttcctta ttacgagtaa ccaggtttct ccgattacgg cgacagcggt ctacagtctg
     1021 gcgaacgcat cgaatacagc tttcagggac tccgggggcg acacaggata cgctatagac
     1081 aatcccgata atggtggctt tgcgatcttt aagacttcca tccccgcaaa ttttgcaatt
     1141 ggaacgcgaa tggattctgt tggcttctcg ggtattgcgg caggtctatt ccgcgaagga
     1201 gccggacttc cgccaatcag cactacgagt ccgacaggcc aggcaagtca tgttcgtttt
     1261 atcggaaggt tgggtgagcc acacgacact ggcgcgaatg aagcagactt catctacgtg
     1321 aatcccgtaa acgaactcag tccaactccg actcagggcg ccgctggacc agaaaatctc
     1381 gatagtccga taatccaaag taatgtggta gacgctttgg ttgcgccttg cattgctgca
     1441 acgctacccc cgaaccgtgt gcgagttgga tcgggcaacg gcggaacact ctctattcgt
     1501 cgcacattgc agaacaacac aggtgctgcg gtgacgaggt tgcgattccg tgtggtcgaa
     1561 atcacaacag cgcctgctcc ggatctggct accgcgattc tggctgtgac ttcttcttcg
     1621 aactcaattg aagcaagtcc atgtggtcca aatgtaacga tcactggatt gactcttgaa
     1681 gacacgccgg tgccagtgca accgcaaggt ggcggccaga acagcacctt gtcggcaggc
     1741 acgatcacgc tcgcggcgcc gctggcaaat gggtcgagta ttagggtcaa ctttttgact
     1801 aacgtcgtgc aggcgggtca cttcacgttc tttgtgaacg tcgaagcgct gccttaaagg
     1861 gcggcagaat tgagatctca gatcacaaag acaacgcacc aagcaaccga gagtcttgga
     1921 attccaccaa cgggagttgg tggttcgtta aagtccaagc tacaaaccga gcaacccgag
     1981 agtctcttgg aattccacca acgggagtta aagtcccacc taacctcaaa gaagtcacaa
     2041 cgcaacagtt tctgggtacc ggtaggtaac aacgtattca ggcggggaac tttctaaaat
     2101 aggttcacct tcaaattcca gccacgcatg gccatccaga ttaccctgag tatctcttcg
     2161 gactccgaat acaattgtcg tgggtatgcc attcttagat aagtaacgcc tcaacacggc
     2221 cgcacgcttc cagcatttcg gtttgagaaa aacaacatca gccgcaagca actgatcaag
     2281 cgtatttgcc agacgcttat cgagatctgc tcggtctcga cttccagcaa tgtccttccc
     2341 ggacacaaac tccagagcct ttggtaacga ccagagtcgg gccaccagcg tgacgacacc
     2401 aatccacgac gccatccgaa acatcagaaa aagctctttc gggtgtcgta ccgttttccg
     2461 agccaatgcg agtgcaagcc gccaactctt atgtgcctgt agttttgcgt ctggcataca
     2521 acgcgttcga ttctatccga tttcagcacc aggcgcagtt acctcgaaca agccttgctc
     2581 atttccctgt gacacaactt tccctgtaat ctgtacgcat acaatttcga gggggtgaag
     2641 ttacaaatga cagagccatc gcgcatacta ctgcagacca ccattccaga tgtcgaagat
     2701 gactggcacg tgggtcgttt ctctctacta cgacaacact tgagttcgct caaagacgac
     2761 caggggaaca gcctgtttgt tgtcgcggcg cggaaccgcc agaccaacgc tgacggcaac
     2821 gatgaggttt taagccaact cgataagagc gactttgatc agctatggat ctttgccgta
     2881 gacaccggca atggactgac cattgcagat tgcgaaggca tcactcgctt ccgccaacgt
     2941 ggtggcggca ttttatcgat gcgcgaccat caggacctcg ggtcgtctct ttgcaccatt
     3001 ggcggcatcg gacgcgcaca ttattttcac tcgaaacaac aggaacccga tgagtcgaga
     3061 agatctcgag atgatcaggc cacagcgcaa atcacctggc ccaattacca ttcgggcaat
     3121 aatggcgact atcaattaat aacgacggtc gagcctgttc acgaattatt acggcgaggt
     3181 gatggcgagg taattaagta ttttccggcc catccccacg aaggcgccgt aggagttcct
     3241 gcggacgaga gtaatgcgcg agtcatcgca acaggcgaga gtcaaacaac acatcgtccg
     3301 ttcaacctgg tggtagcttt tgaaaaggca tcagatagcc acggtaacga gttggggcgg
     3361 gccattgcgg aatcgagttt tcatcacttc gtcgattaca actgggacat ttcaatgggt
     3421 tgccccagct tcctcgttga aacgccgggt caccaaattc aaaacgaacc ggaaaagctg
     3481 tcggacatca aaacttatgt acgcaactta gcgctctggc ttacacctca aagaaattga
     3541 agggtttgca ccgcgtttag gctgactgtt tgcaggatga tcgataactc aactacccaa
     3601 ttggatgtcg ttggttccgg tcgaactcat attgatgtca aagtggatct cattaatgaa
     3661 gacactcctt attttcttcc tcctcatttt catcgtttta tctggacatg gacaccgata
     3721 cgccatcggc caacagcctg ccaactcgcc cgcacgggct gacattcttc gaggtgagta
     3781 tgggcgattc cgagcaaata acga
//
LOCUS       DASSFP010000286         7898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2162486, whole genome shotgun sequence.
ACCESSION   DASSFP010000286 DASSFP010000000
VERSION     DASSFP010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162486"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFH91_06310"
     CDS             <1..829
                     /locus_tag="VFH91_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862638.1"
                     /translation="VAVNQQRLVRFPALIEAILGNPARTPEAERRAKETKVEFFEKER
                     GARQIADELRARGQTAAAEFFEEAELTTDSGGLTLEDAWIIAQHIEVSDADIDESWLP
                     AERYEELVTETEAERIANLQRVVESERLEAGDISMERISLIRRIMLMNTRDRMKLAIK
                     GDREARGILIRDSNKIVSSAVMANPRITEHEIENIAAMRTISDDVLRLIALNRAWARQ
                     YPIIHNLARNPKTPIPTAMNILPRIRTKDLQVLSQSRNVSEAVRRQAYRLSQIRSGE"
     gene            complement(1230..2402)
                     /locus_tag="VFH91_06315"
     CDS             complement(1230..2402)
                     /locus_tag="VFH91_06315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6862639.1"
                     /translation="MRIAILGTRGIPASYGGFETFAEHLSTRLVARGHEVTVYCRAHY
                     VSPRQLEYHGVRLKVLPTIRHKYFDTVVHAFLSAVHAISGRYDAALICNAANAPFSPI
                     LRLTRTPVAINVDGLEHKRKKWGRLGRRYYRLAEYLSTILPNEMVTDAQVIQDYYLAH
                     YKCPSTMIAYGSEVERRPDREAVRKWRVEPNRYVLYVSRLEPENNAHLVIEAFKKVRT
                     AYRLLVVGDAPYAEQYIKRLKAQARGDKRIVFTGFVFGQDYRALQQNAYCYVHATEVG
                     GTHPALLEAMGYGNCVLTLATPENIEAVGEAGIPYIDEFDLAEKLQRVLRDGSLVQAY
                     RNRAQLRVQGNYDWDRVVDKYEDLFSRMSGKPVLPRYQQLQEDLEESRLSRTAATK"
     gene            complement(2435..3595)
                     /locus_tag="VFH91_06320"
     CDS             complement(2435..3595)
                     /locus_tag="VFH91_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6862640.1"
                     /translation="MRLRIAIDAHSVGTKLGGNESYAINLIEALAQVDAVNDYTLFVT
                     TAEAYDRFNQRWTNFKVRSTLPHTPLIRIPLTLSAELRKNPVDVLHVQFTAPPFCPCP
                     IVLSVHDLSFEHLPQTFKRRSRTQLRLTVRHSARRAARVLSLSEHARQDIIATYGIDS
                     DRISAIPLAAPGHFARVTDATELQRVRHTYGIDGDYILSVGSIQPRKNLARLIRAYAL
                     LREKRGKENVPKLVLAGKCAWLFDETLRSLEESGVKDSVILTGYVPEKDLPPLYSGAM
                     CFVYPSFFEGFGLPPLEAMKCGAPVIVSNNTSLPEVVGDAAICVDPFDISAIADALAR
                     VSNDSSLREQLSIKGVARASLFDWRDTARRTLDVYRQVVNESFRSKLTARVN"
     gene            complement(3657..4055)
                     /gene="rplS"
                     /locus_tag="VFH91_06325"
     CDS             complement(3657..4055)
                     /gene="rplS"
                     /locus_tag="VFH91_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET6862641.1"
                     /translation="MNRLDKIEATQLRNNIPQFQPGDTVRVHVRIKESETKERLQAFE
                     GVVIARKHGGARETITVRKTSFGVGVERIFPLHATIIDHIDLIKRGRVRRAKLYYLRE
                     LRGKAARIRERDTRGRQNDEAQASVAGKSK"
     gene            complement(4135..4830)
                     /gene="trmD"
                     /locus_tag="VFH91_06330"
     CDS             complement(4135..4830)
                     /gene="trmD"
                     /locus_tag="VFH91_06330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET6862642.1"
                     /translation="MLRFDIITIFPEFFRDVIDYGIVRRARNAGLVEINAHDLRQWTT
                     DKHRIVDDRPFGGGDGMVLKPEPLFAGVEALTGSHSRDSLATGTRVVLLSPQGQVFSQ
                     AIANELSQAEQIVLLCGRYEGVDERVSEALVTDEISIGDYVLSGGEPAALVLIDAVVR
                     LLPGALGSDTSAVYESFSTGLLDHPQYTRPPEFRGMKVPEVLLSGNHGEIERWRREAA
                     LEKTRRRRPDLLK"
     gene            complement(4882..5415)
                     /gene="rimM"
                     /locus_tag="VFH91_06335"
     CDS             complement(4882..5415)
                     /gene="rimM"
                     /locus_tag="VFH91_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET6862643.1"
                     /translation="MIHGQEENRDLVIVARAVRTRGLKGELVAELLTDFPERFDSLEE
                     IIAVSPGGEQQIVKLENFWFQNDRVILKIESYDDIETAKRLIGFDFAVPEADRVQLEA
                     NEYYDWELEGCAVTTVDGEPVGIVSSVMKTGGTEILVVNDQEVEKLVPLAASIVKDID
                     PAAKRILIDPPPGLLEL"
     gene            complement(5412..5645)
                     /locus_tag="VFH91_06340"
     CDS             complement(5412..5645)
                     /locus_tag="VFH91_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET6862644.1"
                     /translation="MREAIEMVVKGLVDDSETVDIREIEQRNGSTLIEVRVGAGDVGK
                     IIGKQGRTIRALRSLARMAGAKKGKRYQLEIVE"
     gene            complement(5679..5942)
                     /gene="rpsP"
                     /locus_tag="VFH91_06345"
     CDS             complement(5679..5942)
                     /gene="rpsP"
                     /locus_tag="VFH91_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET6862645.1"
                     /translation="MLAIQLMRTGAKKSPSYRVVVKEKRSKRDGAYLENLGTYNPTRN
                     PAEINLKLNRVTYWIEQGAQPTNTVNRLIKASSKAAAAATTEA"
     gene            complement(5983..7458)
                     /gene="ffh"
                     /locus_tag="VFH91_06350"
     CDS             complement(5983..7458)
                     /gene="ffh"
                     /locus_tag="VFH91_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET6862646.1"
                     /translation="MFESLSDKLKRTLKNLRGEGVLTKAHVDAALREIRLALLEADVN
                     YKVAKDFIASVQAKAEGQQVWQELKPSEQVVKIVFDELVELLGGQSSRLVFTKQIPNT
                     VMVVGLQGSGKTTSTGKIARWLATNQNRKPLLLSVDVYRPAAREQLKVVAKTAGQQIF
                     EYPASNDPMTLVREAFKHAQQTGFDTLMIDTAGRLHIDDELMEELVKIKAETHPVETL
                     FVADAMTGQDAVRSAEEFHRRVGITGVILTKMDGDARGGAALSIKQVTGQPVKFVGVG
                     ERYDALEPFYPDRVAQRILGMGDVLSLIEEVQSKVDQSEAEEQLKKLQKNEFTLDDFR
                     SQLRQVKKLGSFSKIMKLLPDQLLGGMGMPQLNEEQSKMMEDELKKTEAIIDSMTWAE
                     RSDHRILNANRRRRIARGSGTAVADVNQLIKQYVDMRQMMRQLSSSGLFGGSGLKGKM
                     MRKMTGLPDMGGFEGEPGALPALPAGPSKKKLKKKRKKRRK"
BASE COUNT         1858 a         2121 c         1897 g         2022 t
ORIGIN      
        1 ggttgcagta aatcagcagc gactcgttcg attcccggca ctgatcgagg cgattctagg
       61 caatccagca cgtactcctg aagccgaacg ccgcgcgaaa gaaaccaaag tcgaattctt
      121 tgaaaaggag cgaggcgcac gacaaattgc tgacgaactc cgggcccgag gacaaactgc
      181 cgcggccgag ttctttgaag aagcagagct aacaaccgac tcgggtggtc taacgctcga
      241 agatgcgtgg atcattgcgc aacatataga ggtatctgac gcagatattg atgagtcgtg
      301 gctacctgca gagcgttacg aggaactcgt aacagagacc gaagctgaga ggattgccaa
      361 tcttcagcgt gtagttgaga gcgaacgtct cgaagccggt gacatttcga tggaacggat
      421 ctcactcatt cgtcgaatca tgctgatgaa cactcgcgat cgaatgaagc tggcgatcaa
      481 aggcgaccgc gaggctcgcg gaattcttat cagagattcc aataagatcg tttcatctgc
      541 ggttatggcg aatccccgaa taactgagca cgagatagaa aacatagccg caatgcgaac
      601 catctcagat gacgttcttc ggcttattgc gttgaacaga gcctgggctc gtcaatatcc
      661 aatcattcat aatctggcac gtaatccaaa aacgccgatt ccaaccgcca tgaatatact
      721 ccctcgcatt cgcaccaagg atttgcaggt cctttcgcaa agcaggaatg tctctgaagc
      781 cgttcgaagg caagcatacc gccttagcca gatccgctca ggcgagtaac gtttcctcac
      841 aaattctcat agaaattagt ggtcgcccgg ctttttagta ggctgaagtt ccaagatcaa
      901 acaacaaagc acaacggaag aagtggcgtc tgtcacatca tctttgcgaa atagttaata
      961 ttatccttga caggtcacaa aacaatcgta taatttcgca cactaactga gtagtacttg
     1021 aaaatcggta acggcgcaga cggcaggcgg caatgaattc caggttttat tcccatgccc
     1081 gcttaacgac cgccccaaaa aagagtcgac cctagaatat cgactcttat gctctgcacc
     1141 gccaccctgt ttgcccgaac ctgccggtaa aattcagccc cgtcaatttt cgaaattgac
     1201 ggggccctat cttattcaat accagtaatt tacttggtag cagccgttct ggataatctc
     1261 gactcttcca aatcctcctg aagttgctgg tagcgcggca acaccggttt accactcata
     1321 cgagaaaaca gatcctcgta tttatcgacg actctgtccc aatcgtaatt tccctgaact
     1381 ctcagctgtg cacggttccg ataggcctgc accagcgagc catcccgcaa aactcgttgc
     1441 agtttttcgg ccagatcaaa ctcatcgata taagggattc ctgcttcacc cactgcttca
     1501 atattttccg gcgttgccaa cgtcaacaca caatttccat aacccatcgc ctctagtagt
     1561 gctggatgag tgcctccaac ttcagtggcg tgaacgtagc agtacgcatt ttgttgcaac
     1621 gcgcgataat cctgcccaaa cacaaatccg gtaaacacta tcctcttatc gccccgcgct
     1681 tgtgctttga gcctttttat atattgttcg gcgtagggcg catcacccac aacaagcagg
     1741 cgatatgcgg tgcgcacttt cttaaatgct tcgattacca ggtgcgcatt gttttctggt
     1801 tcgagccggg acacataaag cacgtaacga tttggttcca ctctccattt gcgaactgct
     1861 tcccgatcgg gtctacgttc cacctcgctg ccataagcga tcatagtcga tggacatttg
     1921 taatgggcca ggtagtaatc ctgaataacc tgcgcatctg tgaccatttc gttcggcagg
     1981 atggttgaaa ggtattcagc cagtcgataa tagcgacgac cgagccgtcc ccacttcttg
     2041 cgcttatgtt ccaggccatc aacattgatg gcaactggag tacgggtgag tctgaggatc
     2101 ggactgaatg gagcattcgc ggcattacag atcaacgcgg catcatatct accggaaatt
     2161 gcgtggactg cggaaaggaa ggcatggacc acggtgtcga aatacttgtg acgtatggtc
     2221 ggtagaacct ttaagcggac gccgtgatac tccagctgtc tgggcgacac gtagtgggcg
     2281 cgacaataaa cagtgacttc gtgtcctcgt gcaaccagcc gggtcgaaag gtgttctgca
     2341 aaagtttcga agcctccata gctggctgga atgcctctcg taccgagaat ggcgatgcgc
     2401 ataatctaca actcgtaggg gagctttgcg acttctagtt tactcgcgct gttagtttgc
     2461 ttctgaacga ctcgttcacg acctgccgat aaacatcaag agttcgccgg gctgtgtcgc
     2521 gccaatcaaa caggctggcg cgcgcaacgc ccttgatgct taactgctct cgtaagcttg
     2581 agtcgttgct tactcttgca agtgcgtctg caatcgcgct tatatcaaat gggtccacgc
     2641 aaattgcagc atcacctact acttctggaa ggctcgtgtt gttgctgacg atcaccgggg
     2701 caccacattt catagcctca agtggaggta gaccaaagcc ttcgaagaac gaaggatata
     2761 cgaaacacat tgctcctgaa tagagagggg gtaaatcctt ttcgggaaca taacctgtta
     2821 agattacgga gtctttgact ccagattctt cgagtgagcg gagcgtctca tcaaacagcc
     2881 aggcacactt cccggccagc actagtttag gcacgttttc tttccctcgc ttttcacgta
     2941 gcagagcata ggctcttatg agtcgggcaa gatttttgcg aggttggatt gagcctacag
     3001 acaagatgta atcgccgtct atcccgtaag tatgtcgcac tcgttgcagt tccgtagcat
     3061 cggtaactcg agcgaaatgg cctggtgctg caagaggtat tgcacttatt cgatccgagt
     3121 caataccgta agttgcgatg atgtcttgcc gtgcgtgctc agaaagagaa agaacgcgtg
     3181 ctgcacgacg tgcggagtgg cgtaccgtta aacgaagctg agtgcgactg cggcgtttga
     3241 aagtttgcgg caagtgttcg aacgatagat cgtgaacact cagtacgatt ggacacgggc
     3301 aaaacggcgg tgctgtaaat tgaacgtgca ggacgtcgac tggattttta cgcagttcgg
     3361 cgctgagcgt taagggaatt cggatcaacg gcgtatgagg aagcgtgctc cgaactttga
     3421 agttggtcca acgttggttg aaacggtcgt atgcttcggc agtagttaca aacagcgtgt
     3481 aatcgtttac tgcatcaacc tgggccagag cctcgatcaa gttgatagca tagctttcgt
     3541 ttccgcccag cttcgtccca actgaatgag catcgattgc gatgcgcaat cgcattctcc
     3601 caagttgcct tggtggatcc ggcgcggatc cacacaagag gtctataaac ttatacttat
     3661 ttgctttttc ctgcgactga agcctgcgcc tcgtcattct gacgaccgcg ggtgtcacgc
     3721 tcacgaatgc gggcagcctt acctcgcaat tcacgaagat aatacaactt cgctctgcga
     3781 acacggcctc tttttattaa atcaatgtga tcgatgatag tggcatgcag ggggaaaatt
     3841 cgttccacgc cgacaccgaa actggttttg cggacagtaa tagtctcgcg ggcgccacca
     3901 tgtttgcgag cgatgacaac cccttcaaag gcttgcagtc gttccttggt ttcagactct
     3961 ttaattctta catgaacgcg gaccgtatcg cccggctgaa attgcggtat gttattccgc
     4021 aattgtgttg cttcaatttt gtctaatcga ttcatctttc aatgctccta tcctcgagcc
     4081 gcgacactct gatccgctca atacctttta accgttcgcg ctctaacaat tcaactattt
     4141 caacaaatct ggtcgccgtc gccgtgtttt ttccagcgcg gcctctctac gccatcgttc
     4201 aatctcgcca tgatttccgc tcaaaagcac ttcggggact ttcatgcccc gaaactcggg
     4261 aggcctcgtg tactgtggat ggtccaacag tcccgtacta aaggattcgt agaccgccga
     4321 agtgtcactt cccaaagcgc cgggcagcaa ccgtacaacc gcatcgatca acacgagcgc
     4381 tgccggttcg cctcctgaga gaacataatc accgatcgat atttcgtccg taacaagtgc
     4441 ttctgacact cgttcgtcaa cgccttcata tctaccgcaa agcaaaacaa tctgctctgc
     4501 ctgcgacagc tcgttcgcga ttgcctgcga aaagacctgg ccttgtggag atagaagaac
     4561 tactcgagtt ccagtggcca ggctatctct gctatgcgag ccagtcagtg cttcaactcc
     4621 tgcgaacagc ggttcgggct taagtaccat tccgtcgccg ccaccgaacg gacgatcgtc
     4681 gacgatccga tgcttgtcag tggtccattg acgtaaatca tgcgcgttta tctcgaccaa
     4741 accagcattg cgcgcacgtc tcacgattcc gtaatcgatt acgtcgcgga agaactccgg
     4801 aaagatcgtg atgatatcga aacgcaacat acgtcttact tactcttcgc agtatcgccg
     4861 catcaggcgt gtctcaaaga tctataactc aagcaggccg ggcggcggat cgatcaggat
     4921 tcgctttgct gccggatcaa tatctttaac tatcgacgcg gcaagaggaa ccagtttttc
     4981 aacttcctga tcattcacca caaggatctc ggttccaccg gtcttcatca cggaactgac
     5041 tatgcctacc ggttcaccat caacggtagt aaccgcgcat ccttccagtt cccagtcgta
     5101 gtattcgttc gcctcgagct gtacacggtc agcttcgggt acggcgaaat cgaatcctat
     5161 caacctcttt gcggtttcta tgtcgtcata cgactcgatc tttagaatta cccgatcgtt
     5221 ctgaaaccaa aaattttcaa gcttgacgat ttgctgttcg ccgccgggtg aaacagcaat
     5281 tatttcttca agggaatcaa accgttctgg aaaatccgtc aagagctctg ctactaactc
     5341 acctttcaac ccgcgggttc tgaccgcccg agccacaatg accaaatcac ggttttcttc
     5401 ctggccgtga atcattcgac aatttccagc tggtagcgtt tgcctttctt ggctcctgcc
     5461 attcgagcca gagatctcaa tgcccgaatc gtacgccctt gtttgccgat tattttcccg
     5521 acatcgccgg cgcctacgcg aacttcgatc agagtcgagc cgttgcgctg ctcaatctct
     5581 cgaatgtcaa cggtttcgct gtcgtcgacg agtcctttga cgaccatttc tattgcttct
     5641 cgcatatgca gtacgcggga tcagtcggaa gatgaaaatc aggcttcggt tgtcgcagcg
     5701 gcggctgcct ttgaactagc cttgatcaat cggttcacgg tgttggttgg ttgggcacct
     5761 tgttctatcc aatacgtgac ccggttcagc ttcaagttga tctcggctgg atttcttgtt
     5821 gggttatacg ttccaagatt ctccaggtaa gcgccatcgc gcttcgagcg tttttcctta
     5881 actaccactc tataagaggg gcttttcttg gcgcctgtgc gcattaactg aattgctaac
     5941 aaaacgttcc tccttcaaat taggccactt cgtggacact atctactttc gtcgcttttt
     6001 tcttttcttt ttcaacttct tcttcgacgg accagctgga agagccggca acgctcccgg
     6061 ttctccttca aatcctccca tgtctgggag tccggtcatt ttgcgcatca tcttgccttt
     6121 gagtccactg ccgccgaaga gtccggaact cgaaagttgc cgcatcatct gccgcatatc
     6181 gacatattgc ttgatcagtt gattgacgtc ggcgaccgca gtgccggagc ctcgtgcaat
     6241 gcgccggcgg cgattcgcat tgagaattcg atgatcgctg cgttctgccc aggtcatcga
     6301 gtcgatgatg gcctcagttt tcttgagctc atcttccatc atcttcgatt gttcttcgtt
     6361 cagctgaggc atccccatgc ctccgagcag ctgatcgggc agaagcttca ttatctttga
     6421 gaaagatccg agtttcttga cctggcgcag ctgagagcgg aagtcgtcga gcgtaaactc
     6481 gtttttttgc agcttcttca gctgctcttc tgcttccgac tggtctactt tggactgtac
     6541 ttcctcaatg agcgacaaga catcgcccat tccaaggatg cgctgggcga cgcgatctgg
     6601 atagaacggt tcgagtgcgt cataacgctc acccacacca acaaacttaa cgggctgacc
     6661 ggttacctgt ttgatcgaga gtgctgcgcc gccacgggcg tcaccatcca tcttcgtaag
     6721 aattacacct gtaattccga cgcggcggtg gaattcctca gccgatcgaa ccgcatcctg
     6781 gccggtcatg gcatccgcga caaaaagagt ttcaacggga tgcgtctcgg ccttgatctt
     6841 gacaagctct tccatcagct cgtcatcgat atgaagacgg ccggcggtat caatcattag
     6901 agtgtcaaaa ccagtttgtt gagcgtgttt gaatgcctct ctgacgagag tcatcggatc
     6961 attgctcgcg ggatattcaa agatctgttg gccggcggtt ttggcaacca cctttaattg
     7021 ttcacgcgca gccggccggt aaacgtcaac cgacaacaaa agtggcttgc ggttctgatt
     7081 cgtcgcgagc caccgtgcaa tttttccggt agaggtggtt tttcctgagc cttgcaggcc
     7141 cactaccatc accgtgttcg gtatttgctt ggtgaagacc aggcgcgatg attggccacc
     7201 cagcagttca accaattcgt caaaaacaat cttaacgact tgttccgacg gcttcagttc
     7261 ctgccagacc tgttgtcctt cggcctttgc ctgaactgag gctatgaagt cttttgcgac
     7321 cttataatta acgtccgctt ccaaaagggc cagacgtatt tcacgcagcg ccgcatcgac
     7381 atgcgccttt gttagtacgc cctcgcctcg gagattcttc agcgtacgct ttaacttgtc
     7441 cgagagcgac tcaaacatat gacccttagg cgcagttccg tggtgcaaac ggaaacgcaa
     7501 atgatagcgg ccaccccaag gggtgtcaag gcagagacaa aagcttctct tttacctgta
     7561 accatttgtg cgttgagcac ttactgatcc ataagaatac ttacatttct cagcgggcgg
     7621 ttgaatggct ctcaacactt ctctgtacgg ttctgctctc ctgtggtaaa tttcatcacg
     7681 aatcagcggt ttaaacggca tcgtcttcca gaggtagaag atttgtggcc gtcccctcca
     7741 tttataacgt agtgaaaact cggagcaatt agtcttttaa gaacgtaaat ggataaacgt
     7801 gtcggttccg gaccccaggg tcccgaccag gaaaagaccc ccaacgagcg aggtgtcgtc
     7861 gatttggctg atgcgcgtgt gcgtctgcga actcgtgc
//
LOCUS       DASSFP010000287         6207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1566843, whole genome shotgun sequence.
ACCESSION   DASSFP010000287 DASSFP010000000
VERSION     DASSFP010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566843"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..854)
                     /locus_tag="VFH91_06355"
     CDS             complement(270..854)
                     /locus_tag="VFH91_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862647.1"
                     /translation="MFCPRCAANQSNDIKFCTVCGANLQAVRQAVESPETGKKFDWAD
                     TWVADMFMQAGAAERRKLEIERIRGITPEVKRLTEMKAGVITSSVGIGLMIFLYVFMK
                     GVGWNVTPETSQILSRVWVAGIIPVFVGLGIILSGVVVGKKLVAETERGQSLAEGDSG
                     PQALKAADTNEFISSPFSVTEQTTKHLRISDRKQ"
     gene            complement(903..1973)
                     /locus_tag="VFH91_06360"
     CDS             complement(903..1973)
                     /locus_tag="VFH91_06360"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET6862648.1"
                     /translation="MRNSDIVIHRLNSQHLSAGKFGNPADVVRWLGAVQAQDYNAAKW
                     ALALRSENATDLSIEQAFNKGEILRTHVMRPTWHLVTPKDIRWMLELTSPRVNAVCGH
                     YYRKFDLDERFFRRAHKVIARELEAEGHLTRVELRDRLLRHRIEPGDATRMSFILLRA
                     ELDGLVCSGPRRGKQFTYELLDRRVPGTKKISRDEALANLTHRYFTSHGPATAHDYAW
                     WSGLAMSDVRSGLELVGKKLQREREHYFSAVDGNGIPDAVLLPPYDEYLVGYTDRSAT
                     VAPISGEINLPNAIFKGVVLVRGLVKGDWRRKNNLKGIVIEVNNWAQISKVLVRALER
                     VATRYRDFIGLPTRIDLRSRRT"
     gene            2167..5061
                     /locus_tag="VFH91_06365"
     CDS             2167..5061
                     /locus_tag="VFH91_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6862649.1"
                     /translation="MKPKPQVTIAHYQIIEPIGAGGMGAVYKAFDNKLQRIVALKVLP
                     LEYVSQQDRRRRFFQEARAASALTHPHILTVYEVGEDDGKPYIAMEYIEGQTIRQKIK
                     TKAMPLNEALEVAIQIADGLAKAHEIGIIHRDLKPENLMLSKDGYAKILDFGLAKLVA
                     QRDRALVAADSEQKTLIRGVETESGTLLGTVNYMAPEQLLGQRVDHRADIFSFAVVVS
                     EMLTGSAPFVHDNRIDTMHAILHREPKLAESFAIPVGLRQILFRSLAKTPKDRYQTIN
                     ELAQELRAIKKDLDLGKSSIVANKSRLVLRRDLSAATGKIDYAADLNEAQYKAVMTTE
                     GPLLIVAGAGTGKTRTLVYRVARLVEIGVKPESVLLLTFTRRAATSMLTRAAALADAR
                     CQRVSGGTFHALGHSVLRTFAEEAGVQKTFTVLDQSDTEDLIDLLRRQIKLTKERRFP
                     RKRTIAAIFSMMVNKMISMKQVLNQAYPEFIDERPNLEELFKSFEGFKRSRQMLTYDD
                     LLVRLREGLEASPTMRDKLSQQYRYIMVDEYQDTNRLQAELVKLMTSQHDNVAVVGDE
                     FQSIYSFRGASHRNMLEFPKLFPKAQVIKLEENYRSTQPILDVANALMGEVKESYKKR
                     LFSRIEGGQTPVVVSAQDENEQSRFVAQRIEELRNDGVPFSEIAVLFRSSSHSFDLEI
                     ELGKQGIPFRKFGGIRFAESAHIKDALSFLRVVVKPSDTLSWFRSLKLIEHVGDATVY
                     QILEHLGVEKKEFRSTRTRQGLFKKLHTFPGRASYKSQLTRLARLFNTVLESKTPNEQ
                     LSAVLRFYRPLMKNKFDDSQRRGRDLEHLQAIAKRYKTIAKLLDDVALDPTEAIQGSI
                     VRRGGGFVTLSTVHSAKGLEWDNLFVIWMTDGWFPSNRFQDEFDDLDEERRLLYVATT
                     RARRDLHFVYPLNVFRGDAYRGDGGFSPVVSRFLEPIPPEILPHASLMGD"
     gene            5178..6029
                     /locus_tag="VFH91_06370"
     CDS             5178..6029
                     /locus_tag="VFH91_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET6862650.1"
                     /translation="MRWRGERESSNVEDRRGVSRGKVAIGGGLGTLVIIILALLFGAD
                     PQQILQQVPQNGPSAPVQSSQPNTQQEDEMKQFVSVVLGKTEDVWTDVFRKNGRQYRE
                     PTLVLFTDQVQSACGISGAAVGPFYCPGDQKVYIDLSFYEELKRRFNAPGDFAQAYVI
                     AHEVGHHVQKLLGISDKVDAMQRRMSEVEANQLSVRLELQADFFAGVFARYVKEQGLL
                     EPGDVEEALRAASAIGDDTIQKRSQGYVVPDSFTHGTSEQRLRWFRKGYDTGDIRQGD
                     TFAATSL"
BASE COUNT         1588 a         1574 c         1498 g         1547 t
ORIGIN      
        1 tgggcaacct attttgtgag cagtacgtac cttgccaagt tggatgtagc gaagcgaaac
       61 tcgtcgcttc ttgccgagac ctgcttccat cccgatcgtg ccccaaacga ttccgggcac
      121 gcgcatttgc aatttcaccg gggttagatc tttgccgaac atcttacgca tggtgttgta
      181 gaaccagcgt agatgccatg gcgcctgttt tttttctaaa ccagttatcc tcgtcgcctc
      241 cacttacctt ccctcgcttg ttaatttcgt tattgttttc gatccgagat gcgtaggtgt
      301 ttcgtagttt gttcggtgac actaaaagga gaagatataa attcgttcgt gtctgcagct
      361 ttcagtgcct gtggtccact atcgccttcc gccaaactct gcccacgttc cgtttcggct
      421 accaatttct taccaacaac aacgccactc aaaataatac cgagtccaac aaaaaccgga
      481 attattcctg cgacccatac ccggctcagt atttgtgatg tctcgggagt aacgttccag
      541 ccgacgcctt tcatgaacac gtaaagaaag atcatcaacc cgattccaac actactcgta
      601 atcactcccg ccttcatctc ggtgagtcgt tttacttccg gtgtgatccc cctgatccgc
      661 tcaatctcta attttcgcct ttccgcggct cccgcttgca taaacatgtc ggcgacccag
      721 gtatcggccc agtcaaattt tttgcctgtc tctgggctct caacggcctg tcgtaccgcc
      781 tggaggttcg cgccgcaaac tgtacagaat tttatgtcgt tgctctgatt ggcagcacat
      841 ctgggacaaa acataaagcg ctcctcctca gtaatgttgc aaacatacgt caattgtaaa
      901 ccctaggttc gtctggatcg caaatcaatc cgggttggca agccaatgaa atcgcggtaa
      961 cgcgtggcta cgcgctccaa tgcccggacc aggaccttac taatctgcgc ccaattattg
     1021 acttcaatca caatcccttt aagattattt tttctgcgcc agtcgccttt caccaaaccg
     1081 cggaccaaaa ccacaccttt aaagattgca tttggcaaat tgatttcgcc tgaaattggg
     1141 gcaacagtag cgcttcggtc ggtgtagcca acaagatatt cgtcgtacgg gggcaataga
     1201 acggcatccg gtattccgtt cccatctact gcggaaaaat aatgctcacg ctcacgttgc
     1261 aacttctttc ccaccagttc aagcccggat ctaacatcac tcatcgccag gccggaccac
     1321 catgcatagt catgtgcggt tgctgggcca tgactggtga aatatctatg cgtcagattc
     1381 gccagcgctt catctcgact tatcttcttt gttccaggga cacgccggtc tagcagctcg
     1441 tacgtaaact gctttccgcg tcttggacca ctgcagacga gaccatcaag ctcggctcgc
     1501 agcaaaataa aactcattcg agttgcatcg cccggttcga ttcgatgacg taacaatcgg
     1561 tcgcgcaatt ctaccctggt taaatggcct tcagcttcca gttcccttgc aatgaccttg
     1621 tgagcgcggc ggaagaatct ctcatcgaga tcaaacttcc ggtaataatg accgcagaca
     1681 gcgttgactc ttggcgacgt taactccaac atccagcgta tgtcttttgg agtaacaagg
     1741 tgccatgtcg gtctcattac atgcgtgcgt agaatttccc ctttattgaa tgcttgttca
     1801 atggacagat cggttgcgtt ctctgatcga agggcaaggg cccactttgc ggcgttatag
     1861 tcctgggcct gaacagcgcc taaccaacgc acaacgtcgg caggatttcc aaacttaccg
     1921 gctgataaat gctggctatt cagccggtga atcactatgt cgctgttacg catgagaaaa
     1981 gcccggatta tttatcattt ctcattttcc ccttatcatt tttcgttcgc ctcgcccgta
     2041 tcctttttta tacggcccga agtaagagaa ttaaggacag atgataaatg gaaaatgacc
     2101 actaacaaat gttttggagg gtcaccttac cgaaaatctt tggtgtaaac tagcgcattc
     2161 ccggtcatga aaccgaaacc tcaggtcaca atcgctcact atcagattat cgaacccatc
     2221 ggcgcaggtg ggatgggggc ggtatataaa gctttcgata acaagctaca gcggattgtg
     2281 gccttaaaag tcctgccgct tgagtatgtc tcacaacaag atcggcgccg tcgctttttc
     2341 caggaagcgc gagcggcgtc cgctttgacg catccacaca tcctcaccgt ctatgaagta
     2401 ggagaggatg acggcaaacc ttacattgcc atggagtaca ttgaagggca aacgatccgc
     2461 cagaaaataa agaccaaagc aatgccgttg aacgaggctt tggaagtggc aattcagata
     2521 gcagacggac ttgcaaaggc gcacgagatc ggaattattc atcgcgattt aaaacctgaa
     2581 aacctcatgc tcagcaagga cggctatgca aaaattctcg attttggact cgctaaactt
     2641 gtggcccagc gtgaccgagc cctggtcgcc gccgacagcg aacaaaaaac attaatcaga
     2701 ggagttgaaa ccgaatcagg gaccctactt ggtactgtga actacatggc gccagaacag
     2761 ctgctgggcc agcgagtgga ccatcgcgcc gatatattct cattcgccgt agtggtgtct
     2821 gagatgctca ccggctcggc tccatttgtt cacgacaatc gcatcgatac gatgcacgcc
     2881 attcttcatc gcgaacctaa actggcagag agttttgcga taccagttgg cttaagacag
     2941 atcctcttcc gttcgctcgc aaagacaccc aaagatcgat atcagaccat caacgaactc
     3001 gcacaagaac tgcgagcaat caaaaaggat ctcgacctgg gtaagagctc gatcgttgca
     3061 aacaagtcga gactagtttt gcgtcgtgac ctctccgccg ccactggaaa gatagattac
     3121 gccgctgact tgaacgaagc tcaatacaag gcggtaatga cgaccgaagg tcctttgctg
     3181 atcgtagctg gtgcgggtac cggaaaaact cgaacgctgg tttaccgtgt cgcccggttg
     3241 gtcgagatcg gtgtgaagcc cgagtccgtt ttgctactga ccttcacgag acgcgcggcc
     3301 accagcatgt taactcgagc ggccgccctc gctgacgctc gttgccagcg agtttcaggc
     3361 ggtacttttc acgcactcgg ccattcagtg ttgagaactt ttgctgaaga agcaggagtt
     3421 caaaagactt ttactgttct cgatcaaagc gacaccgaag atttgatcga tttgcttcgc
     3481 cgtcaaatca aactcacgaa agaaagacgt tttccacgca aacgaaccat tgccgcaatc
     3541 ttcagcatga tggttaacaa gatgatttcg atgaaacagg tattgaacca ggcttatcct
     3601 gaattcatcg acgagcggcc aaatctggaa gagctgttta agtctttcga aggattcaag
     3661 agaagcaggc aaatgcttac ttatgatgac cttctcgttc gcttgcgaga aggtctggaa
     3721 gcgagcccca caatgcgcga caaattgtca caacagtacc gctacataat ggtcgacgaa
     3781 tatcaggata caaatcgtct gcaggcggag ttagtcaaac tcatgacgtc tcagcacgac
     3841 aacgttgcag ttgtaggcga tgagttccaa tcgatctact ccttccgcgg ggccagtcat
     3901 cgaaacatgc tggagttccc aaaacttttc cccaaggcac aggtcatcaa acttgaagag
     3961 aactatcgca gtactcaacc gattctcgat gttgcgaacg ccctaatggg tgaggtcaag
     4021 gagagctata agaagagact gttttcgcga attgaaggtg gacagacacc cgtggtcgtt
     4081 agtgctcagg atgaaaacga gcaatcaaga ttcgtcgccc aacgaataga agagttaaga
     4141 aatgacgggg taccgtttag cgaaattgcc gtcttgtttc gctccagttc ccattcgttt
     4201 gatctggaaa tagagttggg aaaacaaggc attccgtttc gtaaatttgg gggcatacga
     4261 ttcgctgaat cggcccacat taaagacgct ctttcgttcc tccgcgtagt ggtcaagcct
     4321 tccgacactc tttcgtggtt cagatcttta aagttgatcg agcacgtagg cgacgccact
     4381 gtttatcaaa tactcgagca tctgggagtg gaaaagaaag agtttcgttc gacaaggacc
     4441 aggcaaggtc tcttcaaaaa gctacacact tttccagggc gtgcgagtta taaaagtcaa
     4501 ctcactcgac ttgcacgttt attcaacacc gtgcttgaaa gcaagactcc gaatgaacag
     4561 ctgagcgctg tgctgcgttt ttaccgtccg ttgatgaaga acaagtttga cgattcacaa
     4621 cgtcgtggcc gagacctcga gcatctccag gcaatagcaa agcgatataa gacgatcgct
     4681 aaattgctcg acgatgttgc gctcgatccg actgaagcaa ttcagggaag catcgttcgt
     4741 cgaggtgggg gctttgtaac gctgtcaacg gtccattcgg ccaaagggct tgaatgggac
     4801 aatctttttg ttatctggat gacggacggc tggttcccgt caaaccgttt tcaggacgag
     4861 tttgacgatc tggacgaaga gcgccgcttg ctctacgtgg cgacgacaag ggcccgacga
     4921 gatttgcact tcgtatatcc gctgaatgta tttcgcggcg acgcttatcg tggagacggt
     4981 ggattttcac cagttgtttc acgctttctt gaaccaatcc cccccgagat tcttcctcac
     5041 gcttcattga tgggcgattg aaaataggtc ctatatgtac tatctgtcct atcctcatat
     5101 aggtcctata aatcccttgc caatccctca cttctgattg atcataccgc tcaatcacga
     5161 acagggaggt cacaccaatg cgttggagag gcgaacgcga gagcagtaat gtcgaagatc
     5221 ggcgcggtgt ttcaagaggc aaggtggcga tcggaggcgg ccttggaact cttgtaatca
     5281 ttattctggc gttactcttc ggggcagatc ctcaacagat tctgcagcag gttccgcaga
     5341 atggtccttc ggctccagtt cagtcttcgc agccaaacac ccaacaagaa gatgagatga
     5401 aacaattcgt ctcagtcgtc ctgggtaaga ccgaagatgt ttggacggat gtctttcgga
     5461 agaatggcag gcaataccgc gaaccaactc tcgtcctctt caccgaccag gttcaatcgg
     5521 cttgcggcat aagtggagcc gcggttggtc cgttctattg cccgggcgat cagaaggtct
     5581 atatcgatct ctcgttttat gaagagttga aaaggagatt caacgcgcct ggtgatttcg
     5641 cacaagccta cgtgatcgcg cacgaagtcg gacaccacgt gcagaagttg ctcggaattt
     5701 ccgacaaggt agacgccatg caacggcgta tgagcgaagt cgaagctaat caactctccg
     5761 tcagacttga actgcaggcc gatttcttcg ccggtgtctt tgcgcgctac gtaaaggaac
     5821 agggactgtt ggaaccggga gacgttgaag aggcattaag agcagcaagt gcaattggtg
     5881 acgataccat tcagaaacgg agtcaaggtt atgtggtgcc cgattcattc actcacggga
     5941 cttcagaaca aaggcttcgc tggtttagaa aggggtacga cacaggcgat atcaggcaag
     6001 gtgatacgtt cgcagctacg agcctgtgag gacatggtaa ttcgtgcagt gtatcgtcga
     6061 gtctaaaacc aacacattgc tgaggggcgg gttggagtag ggcaaatgat ttgataatta
     6121 tgatttgata tttgagaatt gctaagttgt tcttcgattt gtgcggcaac aaatagaaat
     6181 tctcaaatat tctcaaatat caaatta
//
LOCUS       DASSFP010000288         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1112444, whole genome shotgun sequence.
ACCESSION   DASSFP010000288 DASSFP010000000
VERSION     DASSFP010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112444"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1347)
                     /locus_tag="VFH91_06375"
     CDS             complement(292..1347)
                     /locus_tag="VFH91_06375"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET6862651.1"
                     /translation="MSRNKILVSVAILSLVSSLLLAAGCNRHSTPDLSGGFTPEIQPL
                     PQPLTTYEPMTIPADNPMTPEKVALGRQLFFDERLSFDGSRSCYSCHVCEHGLTDGLP
                     KAIGAGNKQLPRSSPTLWNIGYQKEFYWDGRSPSLEKQALAAWTGANMGAKADEIVAK
                     LNALQGYKAQFQKIFQSDATPDNVVKAIAAFERTIISGNTAWDRWKAGDQSALSESAV
                     RGWNIFQAIKCNNCHDGVLFTDQQYHNVGIGMDQKEPDVGRFKVSNKPEDTGAFKTPT
                     LRDIANTAPYFHDGSVKTLEEAVDTMLAGGKPNDHLDKKNLQPHKLDATMREDVLNFL
                     KSLSVDCRLTKPALPQK"
     gene            1534..2064
                     /locus_tag="VFH91_06380"
     CDS             1534..2064
                     /locus_tag="VFH91_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862652.1"
                     /translation="MQTLSLKVNRLTTASLLLAGFLLMAACNSDKDSSHTTATTYVPP
                     PPQQVLKTEFRDGPRFPAKYETGANVDLNKPLLIFNLQDGKSFSVGEEVVVDFSLANA
                     TLKGDGGDYRIRYIIDDGGMQWLDRWQQVVLTGWTPGEHTIRLELIGPDGWPYRNGDF
                     NVVTKTIGVYRPGGAS"
     gene            2212..>2732
                     /locus_tag="VFH91_06385"
     CDS             2212..>2732
                     /locus_tag="VFH91_06385"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6862653.1"
                     /translation="MCERKAVLSDLNGSILSKVMFIGEAPGRKGADRTRIPFSGDQSG
                     ANFDRFLGSIGLSRQAIFITSAALCNPRTQSGANRKPSKTELTNCSPFLARTIELVNP
                     QLIVTLGSVALEALKLIHYHELTLKDSAATIQSWNGRVLVPIYHPSPQVLASHRREAE
                     QLRDYQVVAKAIL"
BASE COUNT          668 a          665 c          672 g          727 t
ORIGIN      
        1 tcctctgatg tgcctctgtg gtggagcaaa atcctaagaa aaccatcacc acggagaccg
       61 aagagtctca gaggcagcac agagaaggtt cagattagga caccactttc gtcattggag
      121 aattacgatt tgatatttga taattgctaa gaattttacg cgcggtctgc aagtaggaaa
      181 ttgttatcgc taacgcccgc gtcaaaatag aaattatcaa atatcaaatc ataattctcc
      241 aatgattaat cctggccctt gaatcctggc ccttgagtcc tggccctgac cttacttctg
      301 cggcaacgct ggcttcgtca gcctacagtc aactgaaagc gacttcagaa agttcaggac
      361 atcctcacgc atcgttgcgt caagtttgtg aggctgaaga tttttcttgt cgagatgatc
      421 gttaggtttg ccaccggcta acatcgtatc cactgcctct tccagagtct taacgctccc
      481 atcgtgaaag tagggcgccg tgttggcgat atcacgaagt gttggtgttt tgaacgctcc
      541 ggtgtcttcg ggtttgtttg acaccttgaa acgaccaacg tccggctcct tctggtccat
      601 gccaattccg acgttgtgat actgctggtc agtaaacaac actccgtcat ggcagttgtt
      661 acatttaatt gcttgaaaaa tattccaacc gcgcacagca ctctcactca gggcgctctg
      721 gtccccggcc ttccaccgat cccatgcggt atttccgctg ataatggttc gttcaaatgc
      781 tgcaatggct ttcacgacgt tgtcgggggt tgcgtcgctt tgaaatatct tttgaaattg
      841 agccttataa ccttgaaggg cattcagctt tgcaactatt tcgtcggcct ttgcccccat
      901 gtttgccccg gtccacgcag ccagtgcctg tttttcaaga gagggacttc tgccatccca
      961 ataaaactct ttttgatagc cgatattcca cagagttggg ctgctgcgag gcaattgctt
     1021 gttaccagcg ccgatcgctt ttggtaaacc atccgtcaat ccatgctcac aaacatgaca
     1081 ggagtaacac gatctacttc cgtcaaagct gaggcgctca tcgaaaaaca gctgccggcc
     1141 cagcgcaacc ttttctggag tcatcggatt gtctgctgga atcgtcattg gctcgtaggt
     1201 tgtcaatggt tgcggcagcg gttgaatctc aggagtgaat ccacctgaca gatcaggagt
     1261 actatgcctg ttacaaccag cggccagtaa aagactggaa acgaggctaa ggattgcaac
     1321 ggatactaaa attttatttc tgctcatagg ctatgcgagt cgatccgaac tctcgtcctc
     1381 cgtttatttg aatgaagtgc tcgggtaagt tttgcggatt cttacactgg tctatctaaa
     1441 tgtctaggag tttgggacca gttgggccag tgagcagttc taatgactgg ccccttttcc
     1501 taagctgaat agactgtgta tgctggacgg caaatgcaaa cactcagtct aaaggtaaat
     1561 cgtttgacga cagcatcttt gttattagcc ggctttctat tgatggcagc gtgtaactca
     1621 gacaaggata gctctcacac aacggcgaca acctatgttc ctccaccgcc gcaacaggtc
     1681 ttgaaaactg aatttcgcga tggtccaaga tttccggcaa agtacgaaac gggcgcgaac
     1741 gttgatctaa acaagccgtt attaatcttc aatcttcaag acggaaaatc gttttcggtc
     1801 ggggaagaag tcgtcgttga tttttcattg gctaatgcca ctctcaaagg ggatggaggc
     1861 gactacagaa ttcgttacat aattgacgat ggagggatgc agtggctcga tcgctggcaa
     1921 caggtagtat taactggctg gacgccggga gaacacacta ttcgcctgga gttaatagga
     1981 ccagatggct ggccttatcg taacggcgat ttcaacgtgg ttactaaaac tattggagtg
     2041 taccggcccg gcggcgcatc gtgatagtag caagatctga gaccggaact taaagcgccg
     2101 ccaggccggc gcactccagg ggttaactta actgtgcaga attctgactc gagagaattc
     2161 cggtttgctg aacttacggt ggaggtcgcc cattgtacgc tttgtaaagc catgtgcgag
     2221 agaaaggccg tcctgagcga tctcaatggt tccatactct ccaaagtgat gttcattggt
     2281 gaagctcctg gtcgaaaagg cgctgaccgt actcgaatcc ctttttccgg agaccaatcg
     2341 ggggcgaatt ttgatcggtt cctgggttcg attggtttat ctcgccaggc aatcttcatc
     2401 acaagtgcag ctctttgtaa tccgcgtact caatcgggcg ctaaccggaa gcccagtaag
     2461 actgaactga ctaactgctc tccattcctt gcgaggacaa ttgaactcgt aaatccgcag
     2521 ctaattgtga cgttgggttc ggtggcgctt gaggctctca agctcattca ctatcatgaa
     2581 ctcacactga aagattccgc tgcaacgatt cagagttgga atggacgtgt gttagtgccg
     2641 atatatcatc ccagccccca ggttttagcg tcccatcgtc gtgaagccga acagctgcga
     2701 gactatcagg tggttgcgaa agcgattttg ga
//
LOCUS       DASSFP010000289         6152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1409592, whole genome shotgun sequence.
ACCESSION   DASSFP010000289 DASSFP010000000
VERSION     DASSFP010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409592"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..915
                     /locus_tag="VFH91_06390"
     CDS             400..915
                     /locus_tag="VFH91_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET6862654.1"
                     /translation="MRIELANLGDGKGEFSTTYEANQLELEDERVRLATPVSVQGRIK
                     RSGAEVIVSGHINGELFINCDRCLKPLQMPLDNDFELEYVDGSDYEALPDAELTEEQM
                     AMSVFDGVGIDVDEIVKEQVLLAVPTRLLCRDDCKGICPICGSDRNTTDCNCETSSSD
                     PRWDALRSLKQ"
     gene            980..1162
                     /gene="rpmF"
                     /locus_tag="VFH91_06395"
     CDS             980..1162
                     /gene="rpmF"
                     /locus_tag="VFH91_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET6862655.1"
                     /translation="MPNPKRRHSKSRRLKRRAHDALRVPQTNLCPNCREPRMPHRVCP
                     KCGYYKGREVLQIEQA"
     gene            1175..2251
                     /gene="plsX"
                     /locus_tag="VFH91_06400"
     CDS             1175..2251
                     /gene="plsX"
                     /locus_tag="VFH91_06400"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET6862656.1"
                     /translation="MRSQSKNDTRLTGKVVVDAMGSDNAPQVEIDGALAATRDLGVGV
                     ILVGQTHKVEPELRRCGWKGDGDFGIELVEAPEVIAMDDPVAVSVRRKKKSSLRVGSK
                     LVVEGRGDGFVSAGNTGAAMATAKMVIGALPGVDRPALAAMIPTKASKPTLLLDVGAN
                     SECKAHHLVQFAIMGDAYSRSVLGTTRPTVGLMSIGEEEAKGNDLTKEAFPLLRQLEN
                     INFVGNVEGRDVFSGLVDVIVTDGFTGNVMLKLSEGLTEAMLAMLKRELTATAVTKAG
                     AVLARPAFNRIKKRLDYSEYGGAPLLGVSRIVVIGHGRSNARAIRNAIRSVKEFSENR
                     TSERIKQGIAGSAMIQSQAVASTG"
     gene            2327..3313
                     /locus_tag="VFH91_06405"
     CDS             2327..3313
                     /locus_tag="VFH91_06405"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET6862657.1"
                     /translation="MANQRAGILGTGHSYPEGILSNADLAQIVETSDDWITTRTGIKQ
                     RRKAAPGEYTSLFAVRAARQAIERAKLDPSEIDLLLCATVTPDQILPSTGCIIQAELG
                     ANNAAAMDIVAACSGFLYGVSLADSMIRTGQIRNALVIGAEILTQYVDYTDRQTCVLF
                     GDGAGAAVLGPVEGNRGILATKIKSDGRYEEQLFSPGGGTRRRPSAETIAAGDHFFKM
                     KGNELFKVAVRSMAEISRDILQTAGLKTEDVDLFIPHQANQRITEAVADRLNVDLSRV
                     YSNIAVHGNTSSASIPIALDECVESGRVKEGDLVLMTSFGGGVTWGGVLLRW"
     gene            3390..4349
                     /gene="fabD"
                     /locus_tag="VFH91_06410"
     CDS             3390..4349
                     /gene="fabD"
                     /locus_tag="VFH91_06410"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET6862658.1"
                     /translation="MTTDVSTSSIAYVFPGQGSQYPGMGKDLAERFETARLVFEEVDE
                     ALGFKLSSLCFEGPAEELQLTENTQPAILAVSVAAWRAVSSENFPPASFVAGHSLGEY
                     SALVAAGAISLADAARTVRARGRYMQEAVPVGTGAMAAIIGGDVVEIERICNEAAGAQ
                     VCSMANLNAPNQVVIAGNSEAVDRASELLKDVAKRVVKLSVSAPFHCALMKPAQERLA
                     VNLAQIPFENARVPIVTNADAKATTDASALKDALYRQVSAPVRWSESMQLLIGKGIGT
                     FIEVGPGKVLTGLMRQISREVTCLNVEDTAGLESARAKLEGRI"
     gene            4384..4626
                     /locus_tag="VFH91_06415"
     CDS             4384..4626
                     /locus_tag="VFH91_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6862659.1"
                     /translation="MPDQEIENKVKQIIVDELGVDENEVTPNARFIDDLGADSLDTVE
                     LVMRFEEEFGIEIPDEDAEKIQSVRDAYNYIDQHKK"
     gene            4722..5981
                     /gene="fabF"
                     /locus_tag="VFH91_06420"
     CDS             4722..5981
                     /gene="fabF"
                     /locus_tag="VFH91_06420"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET6862660.1"
                     /translation="MTRRVVITGLGLVTPVGNTVEETWTALMSGRSGADYIKRFDAER
                     FSVRFACEVKDFDPLKFIAKKEARKMGAFIHYAIAASIEALADSNFVMTPEGKFPDGI
                     AENAGTYISSGIGDFWAIEREHSKLLDDGPDRVSPFFIVSAIVNLAAGQVSIRFGAKG
                     PNSATATACAAGAHAIGDSFKIIQRGDADLMICGGAESAITPMSVAGFASMRALSTNN
                     EDPIHASRPFERDRDGFVIGEGAGIMILEELESAKQRGARIYAEIVGYGAAADAFHLT
                     MPDETGSGARRVMQRTLRDAQVKPEQVGYINAHGTSTPYNDKFETRAIKDTFGEHAYK
                     LAVSSTKSMTGHLLGAAGGIEGVFSVLALHRNVLPPTINYFNPDPECDLDYIPNEPRP
                     AEVQYVLSNSFGFGGTNAALLFKRYEE"
BASE COUNT         1575 a         1413 c         1711 g         1453 t
ORIGIN      
        1 catgatgcca caatctggcg ccgccaccga gagagtaaaa atcaatcacc tatttgaaac
       61 tcacttatcg agaagaacga cgctgctgtc cggtaccgga aaaagattga agcaaatttt
      121 gagagcagaa gcgctgaagg cgttcagcag tttaaacaat tgaaaggcaa gaagttgcgg
      181 ccccgttttg atgctctact cctttgtccg aaaggttaaa gtccggagag cgccatcgaa
      241 aaattcacct ggtaaagagg cccgaactcc cccgtcccct gtagtttaga gtcctcctgc
      301 gcgaatcccg tgcgttgaca cgcctttatt ggtcgctata atgggccctt tgcgcacaac
      361 gcgccggacg tagctatcgg caacaagtgc gagtttgaga tgcgaattga gctggcaaac
      421 cttggggacg gaaaaggaga attctccacc acgtatgagg ctaatcagct cgaactcgaa
      481 gatgagcgcg ttcgtttggc aactccggtc agtgttcagg gacgaatcaa acgatcgggc
      541 gcagaagtta tagtttcagg ccatattaac ggcgagctct ttatcaactg tgaccgttgt
      601 ttaaagccat tgcaaatgcc gctcgataat gactttgagc tggaatatgt cgatggttcc
      661 gactacgaag ctttgcccga tgctgaattg accgaagaac agatggcgat gtcggtcttt
      721 gacggtgtcg gaatcgatgt cgacgaaatt gttaaagaac aagtgctgct cgcggtgcct
      781 acccgacttt tatgtcgtga cgactgcaaa gggatttgtc cgatttgtgg atctgaccga
      841 aacacaacgg actgtaattg tgaaacatca agcagcgacc ctcgttggga tgcattgaga
      901 agtttgaaac agtagcgaga ggtgaagagc tctttagcga aatgctgcaa gaacatcaga
      961 atttagaaac ggagtaatta tgccaaatcc aaaacggcgc cattcgaaat ctcgacgcct
     1021 caagcgtcgt gcgcatgacg cattaagagt tccacagact aatctttgtc caaattgtag
     1081 agagccacgc atgccacatc gcgtctgtcc aaagtgcgga tattacaaag gacgcgaagt
     1141 actccagata gaacaggctt aaggctcaga accggtgcgt agtcagtcga agaatgacac
     1201 ccggctgacg ggtaaggtcg ttgttgatgc aatgggaagc gataatgctc ctcaagttga
     1261 aatcgacggc gctcttgccg cgactcggga cctgggagtt ggcgtcattc ttgtaggcca
     1321 gacccacaag gttgagcctg agcttcgtcg ctgtggttgg aaaggcgacg gcgatttcgg
     1381 aattgagctg gttgaggcgc cggaagtgat tgcgatggac gatccggtgg ccgtctctgt
     1441 cagaagaaag aaaaagagtt ctttgagagt tggctcgaag ttagtagttg aaggtcgggg
     1501 agacggtttc gtttccgccg gtaacacggg cgcggctatg gccaccgcaa aaatggtgat
     1561 tggcgcattg cctggagttg accgccctgc gcttgccgcg atgattccaa cgaaggctag
     1621 taaaccaact ctactattgg atgttggtgc taactcagaa tgtaaggcac atcatctggt
     1681 ccagttcgca ataatgggcg acgcatattc tcggtcagta ttaggtacaa cgcgtcccac
     1741 agtcgggttg atgagcatcg gcgaagagga agcaaaagga aatgacctta cgaaagaggc
     1801 atttcccttg ttgcggcagc ttgagaacat caactttgtc gggaatgtcg agggaagaga
     1861 cgttttctcg ggtttggtcg atgtcatagt tactgatggc ttcacaggta acgttatgtt
     1921 gaagttatct gaaggcttga ccgaagccat gctggcaatg ctgaagcgag agctaacggc
     1981 aactgccgtc acgaaagctg gcgcagtgtt agcaagacct gcctttaaca ggattaagaa
     2041 acgcttagat tattccgaat atggtggcgc tcctctattg ggtgtgagtc ggatagttgt
     2101 gattgggcat ggccgttcta atgcgcgggc gattagaaat gccatccgca gcgttaaaga
     2161 gttttccgag aatcgcacca gtgagaggat caaacagggc atcgccggca gcgcgatgat
     2221 ccaatcacag gctgtcgcat cgacaggctg aactcaaaac attagtattt ggcttttatt
     2281 ttgatagtgg atactattac ggccccataa gagtttgagg caattgatgg ctaatcagcg
     2341 cgctggaatt cttggcacag gacattcata tcctgaaggt attctttcga atgccgatct
     2401 ggcccagatt gtcgagacat ctgacgactg gattacgact cgtactggaa ttaaacaacg
     2461 tcgtaaagcc gcgccaggag aatacacttc gttgtttgca gtgcgcgccg ccagacaggc
     2521 gatcgaacgc gccaaacttg atccatccga aattgatcta ttgctctgtg caacagtaac
     2581 gcccgatcaa atacttcctt ccaccggttg cataattcag gctgaactcg gggccaataa
     2641 cgcggcagca atggatattg tcgctgcttg ctcaggcttt ctttacggag tatcacttgc
     2701 tgattcgatg attcgtacgg gtcaaatccg aaacgctctc gttatcggtg ctgaaattct
     2761 gactcaatac gttgactaca ccgaccggca aacgtgtgtg cttttcggcg atggagcggg
     2821 tgctgccgta cttggaccag ttgaaggtaa tcgcgggatc ctggcgacga agatcaagtc
     2881 ggatggacgt tatgaagaac aattgttctc accgggtgga ggcacgcgtc gacgaccgag
     2941 tgccgaaaca atcgcagctg gagatcactt cttcaagatg aaggggaatg aacttttcaa
     3001 agttgctgtt cgctcgatgg ccgaaatttc ccgagacata ttgcagaccg ctggattaaa
     3061 gacggaagat gttgatctgt ttattccgca tcaggccaat caacggatta ccgaagccgt
     3121 tgccgatcgc ttgaatgttg atttgtcgcg tgtctactcg aatattgccg tgcacggaaa
     3181 cacgtcgtcg gcatcaattc cgattgcgct tgatgagtgc gtagaaagtg gtcgcgtaaa
     3241 ggaaggcgac ctggtgttaa tgacatcatt cggtggtggc gtgacctggg gcggcgtatt
     3301 gttgcgctgg taaactttgg agtgcgccgg cccggcggcg ctttgttaaa ttagtgcgcc
     3361 cctaaacgtg attggatgga taacaacgat tgacaactga cgtaagcaca tcgtcaatag
     3421 cctacgtctt tccaggtcag ggctcgcaat acccgggaat gggtaaggat ctcgcagaga
     3481 gattcgaaac ggctcgattg gtctttgaag aggttgatga agcgttaggt ttcaagctat
     3541 caagcctttg ttttgaaggt ccagctgaag agttgcaact gacggaaaac actcagcccg
     3601 caattctcgc ggtttcagtc gcagcatggc gtgcggtcag tagtgaaaac tttccgccag
     3661 cctcgtttgt ggccggacac agccttggtg aatactcagc gcttgttgca gcgggagcaa
     3721 tatcgctcgc ggatgctgcg cgcactgtcc gagcgcgagg tcgctacatg caggaagccg
     3781 tgccggtcgg aacgggcgcc atggccgcga tcatcggcgg tgacgtagtt gaaattgagc
     3841 gtatatgcaa cgaggctgcc ggcgcacaag tttgttcgat ggctaacctc aatgcaccga
     3901 atcaggttgt aatagcggga aacagcgaag cggtggaccg cgcttccgaa ctgcttaagg
     3961 acgtcgctaa aagagtagtc aagttaagtg taagcgcccc tttccattgc gcgctaatga
     4021 agcccgctca ggagaggcta gcagtcaacc tggcacaaat tccctttgag aacgccaggg
     4081 tgccgatagt cacgaatgct gatgcgaaag cgactactga tgccagcgcg ttaaaggatg
     4141 cgctctacag acaagtttca gcgccggtgc gttggtccga gtcgatgcag ttgctaattg
     4201 gcaaagggat tgggaccttc atcgaagttg gcccaggaaa agttttgacg ggactgatgc
     4261 ggcagatcag tcgtgaagta acctgtctta acgtggaaga cacagcaggt ttggagtcag
     4321 caagagccaa acttgaagga cggatttaaa cgtttctata aactcacaga ggagcaaaac
     4381 gacatgcccg atcaagagat cgagaataag gttaagcaga taatcgttga tgaacttggt
     4441 gttgatgaga atgaagtcac gccaaatgcc cggttcatag atgacttagg cgcagactcg
     4501 ctggatacag tcgaactcgt gatgcgcttc gaagaggagt tcgggatcga aattccggac
     4561 gaggacgccg aaaagattca aagcgtccgc gatgcctata actatattga ccagcacaag
     4621 aaatagtgat cagtgataag tgatgtaaca aggaagctgg tttgtctgaa cttgttaccc
     4681 acctctgatc acttgtctct ttttaactaa ggagctttaa cttgacacgt cgtgtcgtga
     4741 ttaccggcct cggcctcgtc accccagtgg gtaacaccgt ggaagagact tggactgcgc
     4801 tgatgagcgg ccgcagtggc gcggactaca ttaagaggtt cgacgctgag aggttttctg
     4861 taaggtttgc ctgtgaagta aaggatttcg atcctctaaa attcattgca aagaaagagg
     4921 cgcgcaagat gggcgccttt attcattatg caatcgcagc ttctattgaa gcgctggcag
     4981 acagtaactt tgtgatgaca cccgagggca agttcccgga tggtatcgct gaaaatgctg
     5041 gaacctatat cagtagcggc atcggggact tctgggccat tgagcgcgag cactcgaagt
     5101 tactcgatga tggtccagat cgtgtgtctc cattcttcat cgtttcagcg atcgtcaacc
     5161 tcgccgcggg ccaggtgtca atccgatttg gcgctaaggg accgaattcc gcgaccgcta
     5221 ccgcttgcgc tgcaggcgct catgcaatcg gcgacagctt caaaatcatt cagcggggtg
     5281 atgcagatct gatgatttgt ggcggcgccg aatctgccat aacgccaatg agtgtggcgg
     5341 gttttgcctc gatgcgcgct ttatccacga ataacgagga tccaatccat gcatcgcggc
     5401 ctttcgaacg tgatcgcgat ggattcgtca ttggagaagg tgcaggcatc atgattttgg
     5461 aagagctcga gtctgccaaa caacgaggcg cacggattta tgccgaaatc gttggttatg
     5521 gggcggccgc cgatgctttc catctaacga tgccggatga aaccggttcc ggtgcgagac
     5581 gtgtcatgca gcgcactctt cgcgacgcgc aggtgaagcc tgagcaagtt ggttacatca
     5641 acgcacacgg cacctcaacc ccctacaacg acaagtttga aacccgcgca atcaaagaca
     5701 catttggcga gcacgcatac aaactagccg ttagttcaac taagtcgatg acaggtcact
     5761 tacttggcgc ggccggtggt attgaaggag tcttttccgt tttggctctg catagaaatg
     5821 tactgccgcc aacgatcaac tacttcaatc cagatccgga atgtgacctt gactacattc
     5881 cgaatgagcc cagaccggca gaagttcaat atgttttgtc gaacagtttc ggctttggcg
     5941 gaacaaatgc tgcgctgttg ttcaagagat atgaggaata ggtccaacag gtcgtatagg
     6001 tcctattaac acatgaaaat tattgtaatg atgaagcagg ttgcgaataa agacgcgata
     6061 ctgcgaatta ataaagacgc gagttggatt gaagagggcg acctctcgtt cgaagtgagc
     6121 gagtctgatg gctatgctct cgaagaagct tt
//
LOCUS       DASSFP010000290         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2020134, whole genome shotgun sequence.
ACCESSION   DASSFP010000290 DASSFP010000000
VERSION     DASSFP010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020134"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(792..986)
                     /locus_tag="VFH91_06425"
     CDS             complement(792..986)
                     /locus_tag="VFH91_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862661.1"
                     /translation="MEPDVRNNIESYIEKLREELIQEIQGMNSETNGGDTASSLSMGS
                     FESSLQENIGCSSKFSQCAK"
     gene            1461..>2675
                     /locus_tag="VFH91_06430"
     CDS             1461..>2675
                     /locus_tag="VFH91_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6862662.1"
                     /translation="MPNPKSIRRLKRQVKRHLSPRRIYYFTEGVVLLTVFVLIFSGSR
                     LRFIDGFGRRSDLIVALLLVAAAIPLHFYAKRRLLPRIERRYNPAPYDEHKIFFDLGQ
                     GAREVSSIDQLYENLADKIREAFEASNAAIFVHDEMTGDFNLRVLSAPGETKTKANAK
                     KRLSLGSRAFVVRRLVNLSTPLTLEPHDVETWEQAVNSTLPSLRETRANEHDVLKQVK
                     SRLLVQIRQKDELVGILSLGPRRGEFHYSPSDRELLMSIAAQLGLVIDNARLTERMVA
                     QERLRRELVLAAEVQQRLLPSHPPECKAMEVAGFCEPARGIGGDYYDFINFDNCQMGL
                     AIADVAGKGMPAALLMSTVQATLRSLTARNGAPVPVTPELSQIVGKLNRLLFDSTNGE
                     HYVTFFYATFDQA"
BASE COUNT          648 a          658 c          666 g          703 t
ORIGIN      
        1 ctgctcctca gtcgtttgcc agattccttg ctataccacc ggtccgcggt ccagcagaga
       61 cgctgtccac ggtttttggg gatttaattt ccaaagtgtg ctattgatgt agttttcccg
      121 attcagagaa atattagact actcactatg ttataggttt gctgataaag cctgcgcctc
      181 tcacagtgga caatcttggt gcgacgatga attacagggt caaacagtat agacagattc
      241 ggccacaatc tgatcgtcgt gcactactaa ttgtctttca gcgtttcgac cggatcatgg
      301 cgctcgtcga aaaatcaatt atgttaacgc acgagtcgca gtccttgatc gccgaaactc
      361 gaattcatcg tcaaattagc agggaccttc gacaagctaa aggtcctgtc gctccattca
      421 aaggtccttt ggctcaacgt tccgaaatcg acccaactcg agactaagtt gtcacaactt
      481 tgcttcaggc tctcgcgctc agcggggctt catcatcacc tctcctctgc ttgaaggagg
      541 ttctggccgg ggcaaaactc ggctggttag ggattctaac gaacaggaag ccgtgacgcc
      601 tggttacagc gatccgtttg aatctttcca cgaagaagat tgaacgctac gtgaggttta
      661 ctcgcttgtc agtacgttgg ggtacccaac ccactagatc tctcctaatg atccatactc
      721 gcagcaatca tcttcgttgg gaagcaatgc ttagactttt aagattggtt gacccaggct
      781 caagcgttag gctacttggc gcactgactg aatttggagc tgcagccgat gttttcttgg
      841 agtgacgatt cgaaactgcc catactaaga ctcgaggcag tgtcaccgcc attggtttcg
      901 gaattcattc cttgaatttc ctgaatcaat tcttccctta acttctctat gtagctttcg
      961 atgttgttcc gtacatccgg ttccatacac ttacctcctc tcgtctcagg ctcttattaa
     1021 atgggactat atacgggctt cgtcacaata gtttgacgga aatgttggct gatcggcggt
     1081 tttgttaact cggtgctcag ctcgactaag tcagagcggc agaatctgga attttgaaac
     1141 aaaagtacga acaaccgcac ttaccagtgc cagataccag tgccagatat tagccacctg
     1201 cggtgttaat tccctgaagg aagcgaatta gacaaaagcg attcagtgga tttggctcga
     1261 tcttgtttgg gcgacacgag ctgcatttca tgccccatga gcgtgactcg tatggggaaa
     1321 aataagaaag ttgagcccgg cgtgactgat tgcagtctta taactgacag tgtcacaaca
     1381 atttcagtgt ggcttcagta tttgtctcaa ggtcggatga gtggaggcgg attaaagtga
     1441 gagtcctgcc gcgtgtaagc atgcccaacc caaaatcgat tcgccggtta aagcggcaag
     1501 tcaaacggca tctatctcca cgtcgaattt attattttac ggagggtgtg gttctgctga
     1561 ctgtcttcgt tctgattttc agcggcagtc gactcaggtt catcgacggt tttggacgca
     1621 gatcagacct tatcgtcgct ctcctattag tagcagctgc gattcctctc catttctacg
     1681 cgaagcgacg actcttacct cgaattgagc gtcgttacaa tccggctcct tacgacgagc
     1741 acaagatctt cttcgatctc ggacagggtg ctcgcgaggt aagcagtatc gatcagctct
     1801 acgaaaatct cgcggacaaa atcagagagg cgtttgaggc aagtaacgca gccatctttg
     1861 tgcatgatga aatgacggga gatttcaacc tcagagtgtt gtcggctcct ggagaaacga
     1921 aaactaaggc gaatgcaaag aaacggttat cactcggttc aagggcgttc gtcgttcgac
     1981 gtttggtgaa tctatcaaca cctctcacgc tcgaaccgca tgacgtggaa acctgggaac
     2041 aagctgtgaa ctccacgttg ccatcgctgc gcgagactcg agccaacgaa cacgatgtgc
     2101 tcaaacaggt gaagtcgagg ctgttggtcc agatccggca gaaagatgaa ttagttggaa
     2161 tcctttcgct tggtccacga cgcggcgaat ttcactactc gccatctgat cgggaactct
     2221 tgatgtcgat tgcggcacag ctcggattgg tcatcgacaa tgcaagactt acagaacgca
     2281 tggtggccca ggaaagattg cggcgtgagc tcgtgcttgc ggctgaggtt cagcaacgac
     2341 ttttgccatc gcatcctcct gaatgtaagg caatggaagt cgccggtttt tgtgaacccg
     2401 cgcgtgggat tggaggcgat tactacgact tcatcaactt tgataactgc cagatgggtt
     2461 tagcgattgc cgacgtggcc ggtaaaggaa tgccggcggc gcttttaatg tcaacggtgc
     2521 aagcgaccct ccgcagtttg acggcacgaa atggagctcc tgttccagtt actccggaac
     2581 tgtcacagat tgtcggcaaa ctaaatcgcc tgttattcga ctcgaccaac ggcgaacact
     2641 acgtaacgtt cttttatgcg acctttgatc aggcg
//
LOCUS       DASSFP010000291        14057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2163717, whole genome shotgun sequence.
ACCESSION   DASSFP010000291 DASSFP010000000
VERSION     DASSFP010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14057
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163717"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFH91_06435"
     CDS             <1..583
                     /locus_tag="VFH91_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET6862663.1"
                     /translation="LINEDGRLISQYDKIRLLPFGEYVPLPQWLPGASLIRGLVGDFT
                     PGTRYTVMPIGAVRAGVFICIESAYPSIARRLSYGGAGLLINISNDGYLGPTAVMRQH
                     LSNAVFRAVENGREVLRVTNSGITADIRPDGLVQQPTEAFKPDVRTWTINPALARETF
                     YTRHGDLFVAFCVALSLAAVSLSLLGRNKHSAI"
     gene            678..1788
                     /gene="prfB"
                     /locus_tag="VFH91_06440"
     CDS             join(678..749,751..1788)
                     /gene="prfB"
                     /locus_tag="VFH91_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET6862664.1"
                     /translation="MNETLSIQELRPLFDSLKARVDQLGGFFDVPARREELAKIETQA
                     SAPDFWNNQEAAQKLLQVRSQLEKKIERQEHFVSQMDDAGVLFEFAEEDPASTGELHS
                     LIERLDHEVSQAETEMLLAGDNDQRNAICTIHPGAGGTESQDWAEMLLRMYLKWAEQR
                     EFKTEILEYQPGEEAGLKSVTFRVEGEYAYGLLAAEAGVHRLVRISPFDQAARRHTSF
                     ASLFVYPEIDEDIEVDINEKDLRVDTYRSTGAGGQHINTTDSAVRITHFPTGIVVSCQ
                     NQRSQHQNRAVAMQVLRSRLYELELEKRQAETEKLEANKADISFGSQIRSYVLAPYQL
                     VKDTRTKLERGNVDAVLGGDIDDFIKAYLLARKAA"
     gene            1815..4334
                     /locus_tag="VFH91_06445"
     CDS             1815..4334
                     /locus_tag="VFH91_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET6862665.1"
                     /translation="MSTTQIEERPRVIIPRASRANEIIAILLVATGLLLTLCLVSAAF
                     YPNDPSWNSVGQHETRNWAGFIGANVAATALQFVGLAAYLLPLLLFAAAWRRFRSPRS
                     ASPSTRIAGLVVLVVAVSALLSISHLRPLFDSSVQPGGLLGAIVAEGLASGLNRMGAT
                     VILVAIAATGVLLATNLSFVRLYELIEGLLRTRFAFVGTIPEKFRTWQKARHEQARIK
                     KETKAAIKAEREAARATLRQAQNLSAAERVADFMKEPIAAVPTPSPIKRVETSIPIVE
                     PQASIAAAASAGVGTQAIPTVTSRSTKRSVIPSSTEEDDGAEEMVRGVSVLRTKVVET
                     PTATKLPFDANREVNYSDYTLPPLEFLNEAPPPSEQADAELLSLATRLAEKCKEFNVT
                     GQIKHICPGPVVTTYEFKPDPGVKYSRVVSLVDDLCLALKAESIRIDRMPGKPHVGIE
                     VPNPRRETIFLREVIESRAFRESGSKLTIALGKTIDGLNYIADLAKMPHLLIAGTTGA
                     GKSVGVNSLIVSILYRARPDEVKFILIDPKRLELGLYEDIPHLAAPIIVDPKLASRSL
                     KWAVAEMERRYRDLAGWGVRNIDGYNTEILRRNLVQEYDDNGNPWKPLPYIVIIIDEL
                     ADLMMTSGREVEESITRLAQMARAVGIHLVLATQRPSVDVITGLIKANFPSRISFRVS
                     SKIDSRTIIDTNGAEQLLGRGDMLFLPPGTSRLVRVHGAYIDETEISRIVGHAKTQGP
                     PAYDDTITQSDEEALGLEATEGEHDELFEEALRICVEMKRASTSVLQRRLRIGYGRAA
                     AILDIMEREGLIGQADGSRPRPVLGRAYEMVADWDQQGAEV"
     gene            4491..5795
                     /locus_tag="VFH91_06450"
     CDS             4491..5795
                     /locus_tag="VFH91_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HET6862666.1"
                     /translation="MYTRSDLHFLARSIFNSALKAADARAATYNAIRLNHGGLFVLDS
                     KLALERLYVVGIGKAALSMAMGIDDAMPDHLAEGIVVSPEGSVSPQNSLKNWRLLTGG
                     HPFPDQNSLASARAIFNLLSRVNAERATVLFLISGGGSAMVEWPIDERISLDDLRKTN
                     QLLVTSGASIFEINVVRRAFSAVKGGGLARAVPDAEVITLIISDTNQGDEASVASGPT
                     LAPPSATPGAMEVVSRYHLEDHLPESVLKAIALAPASQMIKPSPYHVLLDNQSALEAA
                     AEEAKTHTFVPVIDHEISEQPIQKGCELLLDHFGTYAKRERFCLISGGEFSCTVRGNG
                     KGGRNLETVLRCAIELDRKPANCDVVVMSCGTDGIDGNSPAAGAVADTTTISRAKAIG
                     LDAHEYLARSDSYSFFEKLGDVIVTGPTGTNVRDLRVLLKTN"
     gene            5909..6481
                     /locus_tag="VFH91_06455"
     CDS             5909..6481
                     /locus_tag="VFH91_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HET6862667.1"
                     /translation="MSTYLRNEENAAVQRTNTSSLTFAELIELLQSQATPPELAQINS
                     WLSAVNTSRAELEPYLGFKEGNYWRHRVCRNEAVEMLVICWRPGQKTPIHDHNGSHGV
                     VRVQEGLMWETIFVYDAERGLCYKSGRECPNGEITGADVPDIHQLGNPEVSGQDLITI
                     HVYAPPLGVLKTYKVGSAQVETYTPNDFPT"
     gene            6517..7305
                     /gene="modA"
                     /locus_tag="VFH91_06460"
     CDS             6517..7305
                     /gene="modA"
                     /locus_tag="VFH91_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6862668.1"
                     /translation="MNHLRRTIVLSVLLILQIVLVNCRSDSRDSGSTSELTVAAAADL
                     TPAFEEIGKLFEDKYKIKVTYSFGSTGMLAQQIENGAPVDLFAAANVSYVDQIESKGW
                     VVPGSKAVYARGRITLWVPKESAIRVEQLSDLTRDEVKRIAIANPDHAPYGTAAREAF
                     ESAGIWSKVQPKLVYADNIRQSLQYAQTGNVEVAIVALSLSKQSDGRWTLIPEELHKP
                     LNQGLAIIKGTKNESGARQFSDFLRGPEGRAVLSKYGFEFPTPN"
     gene            complement(7568..10306)
                     /locus_tag="VFH91_06465"
     CDS             complement(7568..10306)
                     /locus_tag="VFH91_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET6862669.1"
                     /translation="MQVAPHQHSSHPLGFLAIAFIAGILSGEYLRLPLAYSLLLTLVL
                     TATVLAILLCRSSQGKGRPRSAAPTTLLVMALMFLAGASLISVRGSQITSKEIAALID
                     ENPESTFDLTGTLDGPPEFSPSGAYFEICISTISRNGIIFPLNRTVSLFKPFRRTSDQ
                     QELLSLSLHHRDTIRLKASLNRQDKYRNPGGSQLAQYLDRKGYFAIGVIKTPILVVQR
                     RTGFRPTDWLYAWRQILQREIDARFSAETSGVLNATLTGNRYNLSKPTAERFREGGTF
                     HVLVISGVHITFIGAAVLLLVRRLSRKRVLQFTVPVLIVWSYSLAVGADSSVIRSALM
                     FTFVALGQMVFRSASSLNTLGAAALVTLVRDPEQLFDPSWQLTFLSVLAIVAIAWPLM
                     SRLAEIGQWRPAAATPYPPICARWVKDLSDSLFWSEVGFRKHLATTPQRYNLFKCPIA
                     ATLERFYLQRIIRYVFATCVVSVAVAYLLLPFQIVYFHRLSLAGFFLNIVVGFLLALL
                     AVIALVAILLAQVSTMLALPLVKVAEVVCSLMIHSVDPFSSLGLGSLRLPEYSGRFWW
                     VYVIYFVPVLWLVMRLDWWRPLSGLRQRNSRTINVAVALLAHACMTCVLVFHPLSAKH
                     QTGRLQVDFLDVGQGDSALVTMPDGRTLLIDGGGRPRFLSSKGERNEPGDNFETDSRS
                     IGEAVVAEYLWWRGLDHVDYILPTHADADHIDGLNDVLRAFRVRTALVARTPAADGEY
                     AQFSESLKTAGTPLQVIQAGDVINFGGVEVRVLWPPANENPEAASMNNDSVVVVIKKG
                     ERSILFTGDIEKSAEQYLINSGQELKADVVKVPHHGSRTSSTASFVAATKPDWAIISV
                     GKTSMFGHPHVEVVERWQAAGARVLTTGVSGTITISTDGHDLVVDTFVKSVEQSAE"
     gene            complement(10338..11609)
                     /gene="tyrS"
                     /locus_tag="VFH91_06470"
     CDS             complement(10338..11609)
                     /gene="tyrS"
                     /locus_tag="VFH91_06470"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET6862670.1"
                     /translation="MNLFEELKWRGLVHDVTPELPEALEKEHVTGYIGFDPSAASLHV
                     GSLLPIMNLARLQRAGHTPIGIAGGGTGLIGDPSGKTQERQLLSREQVEQNLEGIKEQ
                     LARFLDFQTSANPARIVNNADWLAPISMMEFLRDVGKYFTVNYLLGKEAIKRRLESED
                     GLSFTEFSYPLLQAYDFLMLFDRYNCVLQMGGSDQWGNILAGIDLIRRLRGVRTHGLV
                     SPLVTTSGGVKFGKTEAGAVWLDASLTSPYRFYQFWINTDDRDVIPYLKFFTFLTREE
                     IGEFESSVQSEAHTRRAQRELARYVTEMVHGPTALESALRASEVLFGKEISGLSAQDI
                     QDIFVDVPSSELEQSAIDANLTISDLLARSGLASSKGEARRLIEGGGICLNNRRVADP
                     RQPVRPEDFIDGAVIVLRKGQKHYHLMRIMN"
     gene            complement(11664..12842)
                     /locus_tag="VFH91_06475"
     CDS             complement(11664..12842)
                     /locus_tag="VFH91_06475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET6862671.1"
                     /translation="MNETIELEIERILPGGVGLAHAEGKTFFVMLAAPGDRLRVRVDR
                     TQGNLSFASIVEILSPSPERVEPPCPYFGRCGGCDFQQLNYDAQLKAKADIIRDCLHR
                     ITKLQDLPDIKVTASPQQWRYRGRATWQFDREERSIGYYERGSRRVCDVADCAVLKPQ
                     LQAVLEQVRSTDWSNIPADLKHLEATATEEGSSFSPELGNLRTDVVSLRIARENYSYN
                     AAAFFQANEFLLSSLIDAAIGDESGNVALDLYSGVGLFTIAMARRFSKVIAVESSTAA
                     IRFARANLREADLHNAKVVGVTATEWFRENLPTPGSVDFLLLDPPRAGAESVVIRGIV
                     ELRPARISYVSCDPATLARDLKKLIAGGYELTSVEAFDMFPQTHHVETVAHLSLTDAR
                     "
     gene            complement(12858..13574)
                     /locus_tag="VFH91_06480"
     CDS             complement(12858..13574)
                     /locus_tag="VFH91_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862672.1"
                     /translation="MNYFNYYTEIEDAFVRRRGKHLFLSPLDWALMETWKSQDVPLHI
                     VLRGIEKSFDSFEAKPRKRTVKSLLYCQEEVEAQYAEWIESRVGANPESQAGAVEEDN
                     DRTPFSSVAIKDHLVEKRQQLTKIAGSVTAGDTVLAESMLRAVRILEDLEEEQENSAT
                     VETRKLEDSLTGLERLLNEAMLSASGSELDQLKAEVKAQLKPYRAHMEKSAYQQTFDN
                     LLLKRVRERFNVPRLSLFYL"
     gene            complement(13607..>14057)
                     /locus_tag="VFH91_06485"
     CDS             complement(13607..>14057)
                     /locus_tag="VFH91_06485"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET6862673.1"
                     /translation="AFAAVTKYGAPCIAVDFGTATTFNVVNANREYLGGAIAPGLVIS
                     AEALFNRTAQLPRVKISKPEKAIGSSTTMSMQSGIYYGYAGLVDGLLERIIQELNDSP
                     RVIATGGLANLVAGASRLIEQIDDNLTLEGLRLLHARSEEKNDGAAG"
BASE COUNT         3545 a         3622 c         3562 g         3328 t
ORIGIN      
        1 cctgataaat gaagacggtc gactgatctc tcaatatgat aagattcgcc tgctgccgtt
       61 tggcgaatat gtcccgctcc ctcaatggtt accgggagct tctttgataa ggggacttgt
      121 aggtgacttc acgccgggaa cacgctacac cgttatgcct atcggtgcag ttcgagctgg
      181 agtgttcatc tgcatcgagt cggcttatcc gtcgattgct cgccggctga gttatggcgg
      241 agctgggttg ttgataaata tttcgaatga cggttatctg ggaccaaccg cagtaatgcg
      301 acagcatctt tcaaacgctg tcttccgagc tgtagagaat ggtcgcgagg tacttcgagt
      361 cactaacagc ggaattaccg ctgacattag accagacggt ttggtgcaac aaccaaccga
      421 agcttttaaa ccggacgttc gcacatggac aatcaatccg gcactggcgc gagaaacctt
      481 ttatacgcgg cacggcgatc tctttgtggc gttttgcgta gcgctaagcc tcgcagccgt
      541 ttcgttgagc ttactgggta gaaataaaca cagtgcgata taacgttaac caaaagatta
      601 gagccattga cgcaggtcag taccatagca cggtactaag ctgggccttg agcatttagt
      661 aaatagagag agaaaagatg aacgagactt tatcgattca ggaattgaga ccattgtttg
      721 acagcctcaa agcgcgagtt gaccaactcg ggaggtttct ttgatgtccc ggcccgtcgg
      781 gaagagctag cgaagattga aacgcaggct tctgcacctg acttctggaa caaccaggaa
      841 gccgcgcaaa aactcctgca agtgcggagt cagcttgaga agaagataga acgtcaggaa
      901 cactttgttt cgcagatgga cgacgccgga gtgttgtttg agttcgctga agaagatcct
      961 gcttcgacag gtgaactcca ttctttgatt gagcgacttg atcatgaggt aagccaggcc
     1021 gaaaccgaga tgttattggc tggcgataac gatcagcgta atgccatctg cacaatacat
     1081 cccggcgccg gcggtactga atcgcaggac tgggctgaaa tgctcttgcg aatgtatttg
     1141 aaatgggccg agcagcgaga atttaagact gaaattctcg agtatcaacc aggagaggaa
     1201 gccggtctga aaagcgtaac attccgagtt gagggcgaat atgcttacgg cttactggct
     1261 gccgaagctg gagttcatcg cctggtgcgc atatctccct tcgatcaggc ggcccgtcgc
     1321 cacacctctt tcgcttctct ttttgtctat ccagaaattg acgaggacat agaagtcgac
     1381 atcaacgaaa aagatttacg agtcgataca taccgctcta ccggagcagg cggtcaacac
     1441 atcaacacga ctgactccgc agttcgaata actcactttc cgactggcat tgtcgtttcc
     1501 tgtcagaacc aacgctcgca acatcaaaat cgggccgtgg ctatgcaagt tctccgctca
     1561 cgactctacg agcttgaatt ggagaaacga caggctgaaa ccgaaaaact tgaggcgaat
     1621 aaagctgata tttcttttgg ttcgcaaatt cgaagttatg tgctggcacc ttaccagcta
     1681 gtaaaagaca ctcgcacaaa acttgaacgc ggaaatgttg acgctgtttt aggtggcgac
     1741 attgacgact tcattaaggc ctatttgctc gcacgaaagg ctgcgtagcg tttgacgttg
     1801 gtttcggagt ttgcatgtca acaacacaaa ttgaagagag gccgcgtgtg atcattccac
     1861 gcgccagtcg agcgaacgaa ataatcgcga tcctgctggt ggcaaccggc ttgttgctga
     1921 cgctctgcct ggtctctgcc gcgttctatc caaacgatcc ttcatggaac tcggttggtc
     1981 aacatgaaac gcgaaactgg gccggattca taggagcaaa cgtagcggct acggccctcc
     2041 agtttgtcgg cctcgccgcg tatttgctac cactgttgtt gttcgcagcc gcgtggcgaa
     2101 gatttcgttc tccaaggtct gccagtcctt caactcgcat cgctggtctc gtagtactgg
     2161 tcgttgccgt ttcggccctg ctttcgatct cacatcttcg accgctgttt gattccagcg
     2221 tccaaccggg tggtttgtta ggagcgatcg tcgcggaagg tttggcgagc gggctcaatc
     2281 gaatgggcgc cactgtaatc ctcgtcgcga tcgcggcgac cggagtgttg ctggcaacca
     2341 atctttcttt cgtccgattg tatgaactca tcgagggatt actccgaact cgttttgcat
     2401 ttgttggcac tatccctgaa aaattccgca cctggcaaaa agctcgacac gagcaagcgc
     2461 gcattaagaa agagacaaag gctgccatta aagcagaacg cgaagcggcc cgcgctacgt
     2521 tacgtcaggc acaaaatctg tcagccgcgg aacgagttgc tgacttcatg aaagagccga
     2581 tcgcggcagt accaactcca agtccaatta agcgtgttga gacgtccatt ccaattgttg
     2641 agcctcaagc gtcgattgcc gcggcggcaa gcgccggagt tgggacgcag gctattccaa
     2701 ccgtaactag tcgttctact aagcgttcag ttattccttc atcaaccgaa gaagatgatg
     2761 gcgccgagga aatggtaagg ggcgtctcag ttttacgtac caaggtcgtc gaaacaccaa
     2821 ccgccactaa attgccattt gacgctaacc gtgaggtcaa ctactcagac tacacgctgc
     2881 ctccgctgga gtttctgaat gaagctccac caccaagtga gcaggctgat gcagaattgc
     2941 tgagcctggc gacgcgtctc gcggaaaagt gcaaagagtt caacgtaaca ggccaaatta
     3001 aacatatctg tcctggtcct gttgtaacaa cctacgagtt caagcccgat cccggcgtga
     3061 aatactccag ggtggtttca cttgttgacg atctctgtct ggccttaaag gctgaatcca
     3121 tccggattga tcgaatgcct ggtaaaccgc atgttggcat tgaggttccc aatccgcgtc
     3181 gcgaaaccat ctttctgcgt gaagtaattg agtcacgagc gtttcgcgaa tcaggatcga
     3241 aactgaccat cgctttgggt aaaaccattg atggactgaa ctacatcgcg gatctcgcga
     3301 aaatgccgca cctgctaatc gcgggaacca ccggcgcagg aaaatcagtt ggagttaact
     3361 ctctcatcgt ctcgattctc tatcgagcac gtccggacga agttaagttc atcttgatcg
     3421 atccaaagcg gctcgaactg ggcctgtatg aagatattcc gcatttggcg gcgccgatca
     3481 tcgtcgatcc caagctggcg tctcgctcgt tgaagtgggc tgttgcggaa atggaacgca
     3541 ggtatcggga cctggctggt tggggcgtca gaaatatcga tggttacaac acggaaattc
     3601 tccgtcgcaa tctcgtccag gagtacgacg ataacggaaa cccgtggaaa ccgttgccct
     3661 acatcgtaat catcatcgat gaactcgcag atctgatgat gacttctggt cgggaagtcg
     3721 aagaatcaat aaccagattg gcccagatgg ctcgcgctgt cggaatccat cttgtacttg
     3781 cgactcaaag accgtcggtt gacgtaatca ccggcctgat caaagcaaac ttcccttcgc
     3841 gcatttcatt ccgagtttcg tcaaaaatcg actcgcgaac aattatcgat acgaatggtg
     3901 ctgaacagtt actaggtcgg ggcgacatgc tgttccttcc tccaggaacg tcccgacttg
     3961 ttcgcgttca cggtgcttac atcgacgaaa ctgagatcag tagaatcgta ggccacgcta
     4021 agactcaagg tccgcctgca tacgatgaca ccatcacgca atcggacgaa gaggcactcg
     4081 gacttgaagc aacagaaggc gaacacgacg aactgttcga agaggctctg cgcatctgcg
     4141 ttgaaatgaa gcgagcttca acctcagtat tacagcgccg gttgcgtatc ggttatgggc
     4201 gtgcagccgc aattcttgac atcatggaac gcgaaggatt gatcggacaa gctgacggct
     4261 cgcgtcctcg gcccgttctt ggtcgagctt atgaaatggt cgccgattgg gaccaacaag
     4321 gtgcagaagt ttagggagta gggtttaaca ctttcgtgaa aatccactga tccttgtgga
     4381 aaaccctgtg gagaaattcc gtgttaacga gttaagtttt tgattcgtag gaacttctcg
     4441 cagaatgcac acttgaaagg cgttcaccaa ctgaccgggg tctgaactct ttgtacactc
     4501 gttctgatct tcacttctta gctcgttcaa tcttcaactc cgcacttaaa gccgcagacg
     4561 ctcgagctgc aacctataac gcaatcagac taaatcacgg agggcttttc gtcctcgatt
     4621 cgaaattggc cttagaacgt ctctacgttg tcggcattgg gaaagcagct ctgtcgatgg
     4681 caatgggaat tgacgacgcc atgccagacc acttagcaga gggaatcgtt gtcagtcccg
     4741 aaggttctgt atccccacaa aactctctga aaaattggcg actcttaacg ggcgggcatc
     4801 catttccaga tcaaaacagc ctcgcgtcag ctcgcgctat ctttaatcta ctctcacgcg
     4861 ttaacgcaga gcgcgcaact gttctcttcc tgatttcagg cggcggttcc gccatggttg
     4921 aatggccaat tgacgaacga atttcgctcg acgatttacg aaagactaac caactcctcg
     4981 tcaccagtgg cgcgagcatt ttcgaaatca acgttgtccg acgcgccttt tcagcggtaa
     5041 aaggtggagg actcgcgcga gccgttcctg acgctgaagt gatcacgctg atcatttcgg
     5101 acaccaatca gggagatgag gcgagcgtgg cgtcaggacc gacactagcg ccgccttcag
     5161 caacgcccgg tgcgatggaa gtagtttcgc gatatcacct cgaagatcac ctgccagagt
     5221 ccgtgttgaa ggcaattgcc ctggcaccag cttctcaaat gattaaaccc tcgccgtacc
     5281 atgtgttgct ggacaatcag agcgcacttg aagccgctgc agaagaagcc aaaacacaca
     5341 catttgtacc cgtcatcgac cacgaaatta gtgaacagcc aattcagaaa ggatgcgagc
     5401 ttctactgga ccacttcggt acctatgcga agcgagaacg tttctgcctg atctctggag
     5461 gtgaattctc ttgtacggta cggggtaatg gaaagggcgg gcgaaatctg gaaacggtgc
     5521 tgcgttgcgc tatagagctt gatcgcaaac ctgcaaactg cgacgttgtc gtaatgagct
     5581 gcggtactga tgggattgac ggtaacagtc cggcggctgg cgccgttgcg gatacgacaa
     5641 ccatttcgcg agcaaaggca attgggctgg atgctcacga gtatttggct cgcagcgact
     5701 cttattcgtt ttttgaaaag cttggggatg tgattgttac aggcccgaca ggaaccaatg
     5761 tcagggattt gcgagtgcta ttgaagacga actgagattt atttttgatg aggctgattc
     5821 ccatgcttga ctcgacgggt ggcacgacat ataacaaatt tgtcgctgat ttctttggcg
     5881 atttcaaatt cgcgattgga gctcaaattt gtcgacttat ctcagaaacg aagaaaatgc
     5941 ggccgtccaa cgcaccaata cctcatcact cacgtttgct gagctaattg agttgttgca
     6001 aagccaggcg acaccacccg aactcgcaca aataaactca tggctctcag cggtcaacac
     6061 ctcaagagct gagctcgagc cttatttggg atttaaagaa ggcaactatt ggcgtcatcg
     6121 cgtttgcaga aatgaagcgg tcgagatgct cgtcatctgt tggcgaccgg gtcagaaaac
     6181 tccaatccac gatcacaacg gatcccacgg cgttgtgcga gttcaagagg gcttaatgtg
     6241 ggaaacaatt tttgtctacg acgctgagcg cggactctgt tacaaatctg gtcgcgagtg
     6301 tccaaacgga gaaataacgg gcgccgatgt tcccgacata catcaattag gcaatcctga
     6361 ggtcagtgga caggatttga taacaattca cgtctatgct ccgccgctag gcgtgctaaa
     6421 aacctataag gtcggtagcg cacaagtcga aacttacact cctaacgatt ttccaaccta
     6481 acgaacgtgg acgtgggcaa cgcaaggcac ggcaaagtga accatttgcg tcgcacgatc
     6541 gttttaagtg tcctgctgat tcttcagatt gtcctcgtca attgtcgctc tgattcacga
     6601 gacagtggat caacaagcga gttgacggtg gcggcagctg cggatttgac tcctgcgttt
     6661 gaggagatcg gcaagttgtt tgaggacaag tacaagatca aggtgaccta tagcttcggc
     6721 tcaactggaa tgttggccca acaaatcgaa aacggcgcgc cggtagatct cttcgctgca
     6781 gcaaatgtca gctatgtcga tcagattgag tccaaaggct gggtagtgcc tggttcaaaa
     6841 gccgtctacg cacgtggacg aattactctc tgggtaccga aggaaagcgc tattagagtt
     6901 gagcagctca gcgacctgac tcgtgacgag gtcaaacgaa tcgcaattgc caatcctgac
     6961 catgcgccct atggaactgc ggctcgagag gcttttgaaa gcgctggcat ctggtcgaag
     7021 gttcaaccga aactggttta cgctgacaac attcgacaaa gtcttcaata cgcgcagacg
     7081 ggaaacgtcg aggttgcgat agtggccctc tcgctgagca aacagagtga cggtcgctgg
     7141 acgctcattc ctgaagaact gcacaaacct cttaaccagg ggctcgcaat catcaaggga
     7201 acaaaaaacg agagtggggc tcgccaattt tcagattttc ttcgtggccc cgaaggtcgc
     7261 gcagtgttga gcaagtacgg tttcgagttc cccaccccca attaaccctt gcatacttag
     7321 gtagggtcga ttgagttcga gaagtgggtg gtcgttaatt gttgggaagg aagcaaaccc
     7381 tctcattctc accgggcttc agcccggtgt aacgaatcgg aaaaatgacc gtcgaaccgt
     7441 ttcaacggtt tgtcctgcct gaccactcct gacccgttga gaaaccgtta aaacggttcg
     7501 ccaacaattt gggccttgcg acaccgggct aaagcccggt gagaatgaga ccgctttggt
     7561 cctccgatca ttccgccgat tgttccactg attttacaaa cgtatcaact accaaatcat
     7621 gaccgtcggt cgaaatggtg atcgtgcctg acacaccagt cgttaatacc cttgcgccag
     7681 cagcctgcca acgctcaacc acttccacgt gcggatgacc gaacatggat gtttttccga
     7741 cggagatgat ggcccaatct ggcttagtcg ctgctacgaa agatgctgtt gaagatgttc
     7801 gactaccgtg atgcggtacc ttcaccacat cagctttcaa ctcctggccc gaattgatta
     7861 ggtactgctc agcgcttttt tcgatgtcgc ccgtgaaaag gattgaccgt tctcccttct
     7921 taattaccac aaccaccgaa tcgttgttca tcgacgcggc ttccggattc tcattggctg
     7981 gtggccacag cactcgcact tcaacgccac caaaattaat cacatctcca gcctgaatca
     8041 cttgcaatgg agtacctgct gttttgaggg actctgaaaa ttgcgcgtac tctccgtctg
     8101 ccgcaggagt tcttgcgacc agagccgtcc gtactcggaa agctctgagt acgtcgttca
     8161 gcccatcaat gtgatctgcg tccgcgtgag tcggcaatat gtaatcaacg tgatcaagac
     8221 ctctccacca caaatactcg gccacgaccg cttcgccgat acttcgtgaa tccgtttcga
     8281 aattatcgcc cggttcattc ctttcaccct ttgaagaaag gaaccggggt ctgccacctc
     8341 catcgattaa gagagttctg ccatcgggca tagtcacaag cgccgagtct ccttgtccga
     8401 cgtcgagaaa atcaacctga agccttccag tctgatgttt cgcacttaag ggatgaaaca
     8461 ctaagacaca ggtcatacac gcatgggcaa gtagtgccac tgcaacgttg atggtcctgg
     8521 agtttctttg tcgtaatccg gagagcggtc gccaccaatc caacctcatg actaaccaaa
     8581 ggacaggaac aaaatagatt acgtagaccc accagaaacg gcctgagtat tcaggaaggc
     8641 gtagcgaacc taaacctaag cttgaaaatg gatcgacact atgaatcatc aaagagcaaa
     8701 caacttccgc tactttcaca agaggcaaag cgagcatcgt actaacctgg gccaataaaa
     8761 ttgccacgag cgcaattacc gccaacaggg caagtaaaaa ccccacgacg atattaagaa
     8821 aaaagcctgc gagcgagaga cgatggaagt acacaatttg aaatggcaga agcaggtacg
     8881 ctaccgcgac cgaaacaacg caggtcgcaa acacgtaacg gatgattctt tgcaaataaa
     8941 accgttcgag tgtggcggca attgggcact taaataggtt gtatctctga ggtgtcgttg
     9001 ccagatgctt ccgaaaaccc acttcactcc aaaataaact gtctgaaaga tccttaaccc
     9061 aacgcgcgca aattggtggg tatggcgtcg ctgcagccgg tcgccattgc cctatttcgg
     9121 cgagcctgct catcagcggc caggcaatgg cgacgatcgc aagtacagat aagaaggtta
     9181 attgccatga tggatcgaaa agctgttctg gatctctgac gagggtcact aaggcggccg
     9241 ccccaagcgt attcaatgaa gaagcagatc taaagaccat ttgcccaagc gcgacaaaag
     9301 taaacatgag cgccgatcga atcacagacg agtcagctcc gacagccagg gaataggacc
     9361 agacaatcaa gaccgggact gtaaattgaa ggactctttt gcgactaaga cgtcggacca
     9421 atagcaagac agctgcgccg atgaacgtta tgtgaactcc actgattacg agaacgtgga
     9481 aggtgccgcc ttcgcgaaat cgttctgcgg tcggtttcga taggttgtac cggtttccag
     9541 ttaatgtggc gttgagtacg ccagatgttt ccgcggaaaa gcgtgcgtcg atttcgcgtt
     9601 gcaagatttg ccgccaggcg tacagccagt cagttggacg aaaacctgtt cgccgctgca
     9661 caacgaggat tggagttttg atgactccaa tcgcaaagta ccctttgcga tcgagatatt
     9721 gggcaagctg gctgccgccc gggttacggt atttgtcttg acgattcagg gaggctttga
     9781 gacgaatcgt gtcgcggtga tggagactca gacttagtaa ttcctgctga tcggatgttc
     9841 gcctgaacgg cttaaacaac gacacggtac ggttcagtgg aaatattatt ccgtttcgac
     9901 tgattgtgct gatgcatatt tcaaaataag cgccactcgg cgaaaactca ggtggtccat
     9961 caagagttcc cgtcaaatca aaagttgact ccggattttc gtcgatgagt gccgcaatct
    10021 cttttgaagt gatttgcgat cctctgaccg aaatcagcga cgctcccgcc agaaacatca
    10081 gcgccatgac gagcaacgtc gtaggggcgg cactccgtgg ccgccctttc ccttgagaag
    10141 aacgacataa caagatcgcc agcaccgtcg cggtcagtac gagtgtcagg agaagtgaat
    10201 aggcgagggg caaccgtaag tattcgccag aaagaattcc tgcgatgaag gcgatcgcca
    10261 aaaagccgag aggatgcgag ctgtgctggt gaggagcaac ttgcataaaa cgtaaattta
    10321 gctgacgtag acttaactca attcataatg cgcattaaat gatagtgctt ctgacccttg
    10381 cgaagaacaa ttactgcgcc gtcgatgaaa tcctccggcc tcactggttg ccgggggtcc
    10441 gctacgcgac gattattgag acagatacca ccgccttcaa tcaacctgcg ggcttcccct
    10501 ttcgaagagg ccaggccgga ccgggccagc aaatcgctta tcgtgaggtt ggcatcaatt
    10561 gccgactgct caagctccga cgaaggcaca tccacaaaga tgtcctgaat atcttgcgcg
    10621 ctcagtccac tgatctcttt gccgaacaag acctcagacg ctcgcaaagc cgactccaac
    10681 gccgttggac catggaccat ctcggtgacg tagcgggcca gctctcgttg ggctctccga
    10741 gtatgcgcct cggattgaac agacgattca aactcgccga tctcttctcg cgtaaggaag
    10801 gtgaagaatt tcagataagg aataacatca cgatcgtccg tgttaatcca aaactgatag
    10861 aaccggtaag gcgaagttag cgaagcatca agccaaacag caccagcttc agtcttcccg
    10921 aacttcactc caccggatgt cgttaccagg ggtgaaacca ggccgtgagt tcgcacgcca
    10981 cgcaagcgcc gaatcagatc gatccccgcc agtatgttgc cccattggtc gctgcctccc
    11041 atttgcagaa cacagttgta acgatcgaaa agcattagga agtcgtaggc ctggagtaac
    11101 ggataactga attctgtaaa cgagaggccg tcttcgctct ctaaccgccg cttgatggct
    11161 tctttgccaa gcaaataatt gacagtaaag tattttccga cgtcacgtag gaattccatc
    11221 atcgaaatcg gagcaagcca atcggcattg ttcacgattc gtgccggatt ggcgctcgtc
    11281 tggaaatcga ggaaccgggc cagctgttct tttattccct ctaagttctg ttcaacttgt
    11341 tcacgggaga gcagttgacg ttcctgagtt ttgccgcttg gatcaccgat tagaccagtg
    11401 ccaccgccgg caattccaat tggagtgtgg ccagcgcgtt gcaaacgtgc gaggttcatg
    11461 attggcaaca gagagcctac gtgcaaactg gctgcagacg ggtcgaagcc gatgtaaccg
    11521 gtgacgtgtt ccttttcgag cgcttcaggt agctcggggg taacatcatg gaccaggccg
    11581 cgccatttta attcttcgaa aagattcatt gtttgagttg gtctttggtc ttccaaatat
    11641 ctccttgccc taactacaaa ttactacctc gcatcggtca gtgacaaatg tgccacggtc
    11701 tccacgtgat gagtttgagg aaacatgtca aaggcttcca ctgaagtgag ctcatagcca
    11761 cccgcaatta gcttcttaag gtcacgcgcc aaagttgccg gatcgcatga cacatagcta
    11821 attcgagccg gccgcaattc gactatccct cgaatgacga ctgattcagc tccagcacgc
    11881 ggtggatcca gcaacagaaa atcgaccgaa ccaggcgtgg gtaaattctc acggaaccac
    11941 tccgtggccg tgacgcccac aactttagca ttgtgaaggt ctgcttctct tagatttgca
    12001 cgagcaaacc gaattgctgc agtactcgac tcaactgcaa tgacctttga aaagcgtcgg
    12061 gccattgcga tagtaaatag tcccacgccg ctatataaat cgagtgcaac gtttcctgat
    12121 tcgtcgccaa tcgccgcatc gattagagat gacagaagaa attcattagc ctggaagaaa
    12181 gcggccgcat tgtaagagta attctcacgc gcaatgcgta aactcactac atcagtccga
    12241 aggttgccca attctggcga gaacgaactt ccctcttctg tggctgtcgc ctcgagatgc
    12301 ttgagatccg ccggaatgtt agaccagtcg gttgatctaa cttgttccaa caccgcctgt
    12361 aactgaggct tcagtacggc gcagtcagca acgtcacaga ctcgtcggga tccgcgctcg
    12421 taatagccga tcgacctttc ttcgcgatcg aactgccatg tggcccgtcc acgatatcgc
    12481 cactgttgcg gagaagcggt aaccttgatg tcagggagat cctgcagttt ggtaattcgg
    12541 tgaaggcagt ctcgaataat gtcggccttc gctttcagct gcgcgtcata gttgagctgt
    12601 tgaaagtcgc agccgccaca gcgtccaaag taagggcagg gtggctcaac tcgctcagga
    12661 gagggcgata ggatctcaac gattgaagcg aaggataaat ttccctgcgt tcgatcgact
    12721 cgaactcgaa gccgatctcc tggcgctgcc agcattacaa aaaacgtctt accctctgca
    12781 tgggccaaac ccacgccgcc gggaagaatt cgttcgattt ccagctctat tgtttcgttc
    12841 atcaactgct tccgaattca aagatagaac aaactcaaac gcggaacatt gaatctttcg
    12901 cgcacccgct ttaataacaa gttgtcgaac gtctgttgat acgcgctctt ttccatgtgg
    12961 gcccggtatg gtttcaattg agcttttact tcggccttta gttgatcgag ttcgctgcct
    13021 gaggcagaaa gcatcgcttc attcagcagt ctttcgagtc ctgtcaggga atcctccagc
    13081 tttcgagttt cgaccgttgc agagttctct tgttcttctt caaggtcttc cagaattcgg
    13141 acggcacgaa gcatcgactc agccagaaca gtatcaccgg ccgtaacgga ccctgcgatc
    13201 tttgttagct gctgtcgttt ttcgaccagg tggtccttga tggcgacgct cgaaaatggg
    13261 gtgcgatcgt tatcttcctc cacggcaccg gcctggctct ccgggttggc gccaactctg
    13321 gattcgatcc attcggcgta ctgggcttca acctcttcct gacagtacaa caaagatttc
    13381 accgttcttt ttctgggttt agcctcaaag gaatcgaatg acttttctat ccctcttaaa
    13441 actatatgta gcggcacgtc ctgcgacttc caggtttcca ttaacgccca gtccaatgga
    13501 ctgagaaaga gatgtttccc tcgacgtctg acaaaggcgt cttcgatttc cgtgtaatag
    13561 ttgaagtagt tcaatgcaat actcgaagtt ctgatttgga gtgagctcag cccgctgcgc
    13621 cgtcattctt ttcttcagag cgagcgtgca gaagacgcaa accttccagg gttaagttat
    13681 catcgatctg ctcgattaat ctggaagcac ccgccactaa gttagctaaa ccgccagtcg
    13741 cgatcacgcg aggactgtcg ttcagttctt gaatgattct ttcaagcaac ccatcgacga
    13801 ggccagcata tccgtagtag atacctgact gcattgacat agttgttgac gacccgattg
    13861 ctttttccgg tttggagatc tttacgcgcg gaagctgggc tgttcgatta aacaacgctt
    13921 cggcagagat caccaatccg ggcgctattg ctcctccaag gtattcacga ttcgcattga
    13981 cgacgttaaa agtggtggca gtaccaaaat ccacggcgat gcagggtgct ccgtattttg
    14041 taacggccgc aaacgca
//
LOCUS       DASSFP010000292         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1272212, whole genome shotgun sequence.
ACCESSION   DASSFP010000292 DASSFP010000000
VERSION     DASSFP010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272212"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1610
                     /locus_tag="VFH91_06490"
     CDS             333..1610
                     /locus_tag="VFH91_06490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6862674.1"
                     /translation="MIDEVQSTRLPNGIAVLTEHMPGLRSVTVGIWVRRGSRHETPDL
                     NGICHFIEHAVFKGTQRRSAHDIAVETDRLGGHFDAYTTHEMTGFALKVADTSLPEAV
                     DLLADLIAHPRFDQEDLEREQKVILEEMKMVEDTPDELLGELFNAAYFPNHPLGRPIE
                     GTRETVPTFDHKTTVAFHAREFSYTNLVVAAAGNVNHSRLVSLIGEAFGGCEGVGSRP
                     HQISTSIPHPSAPILIEQKNELEQAHLVIASPWPDALSDNRYAASLLASVIGGGTSSR
                     LWQKVREERGLAYSVGAGGSAFTDVGVFNVYAGTSPEQLDEVLDLALIELRRVVQDCV
                     SEEELRIAKEQAISSILLGLESSSARASTLARQEIVHGRRISPDEVIERIRATRSDEL
                     REVARTYFRSEAISLGALGNLNGFRVDRSRLSM"
     gene            1717..>2307
                     /locus_tag="VFH91_06495"
     CDS             1717..>2307
                     /locus_tag="VFH91_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6862675.1"
                     /translation="MKLTVLGSGSSGNAVLIVAGNTRVLVDAGLSAKEITRRLSLVGE
                     DVQQLDAVLVTHEHGDHAGGLRVLLQSVDCPVYISAQTLDAYVSERFNVNNEEPKRRA
                     KALSNRVEQIESSRDFRIGEIDFHPFTIPHDAVDNFGFTATHQGVKIATVMDFGHVTT
                     LMTERLRGCAAIVIESNHSRDMLKACDSYPWELKQRI"
BASE COUNT          551 a          613 c          590 g          553 t
ORIGIN      
        1 cttctcatgc ggtaaaacct ccaggcgcaa aactacctag cggatgcagt tgaacgaggc
       61 aagagccggt caaagattga ttggactggt acaaatgact cctaagactt taactttcca
      121 cggtcacggt gccgttgccg gaggtgtgaa aagccaatcg gttcgggaag ggagacttac
      181 cggaacccac aatagggatt cattttcagg caattgttcg aaatatgtca agtaaatgaa
      241 cggttatacc gcgcctttgc tcatggcttt tagggcatgc taggattcaa cacccctcca
      301 acgcacatat ctcgttacac tggtcgcgac ctatgataga tgaagttcaa tcaactcggc
      361 tccccaacgg cattgcggtc ctgacagaac acatgccggg tctccggtct gtaaccgtcg
      421 gtatatgggt ccgtagaggc tcacgacatg agactcccga cctcaacggc atctgccatt
      481 ttattgagca cgctgtgttt aagggaacgc agcgtcgatc cgctcacgat attgcagtgg
      541 aaactgaccg ccttgggggc cacttcgacg cctataccac tcatgagatg accgggttcg
      601 ctctaaaggt tgcggacacg tcattgccag aagctgttga tcttttggct gacctcatcg
      661 cccatccccg tttcgatcag gaggacctcg aacgggagca aaaagtgatc ttggaagaga
      721 tgaaaatggt ggaggacacg ccggacgaac tgcttgggga gttgttcaac gcggcatatt
      781 ttccgaacca tccgctcggc cgccccatcg aaggcacccg cgaaacagtt ccgacgttcg
      841 atcacaaaac cacagtggcc ttccatgcgc gcgagttttc ctacacaaat ctggttgtcg
      901 cagccgctgg aaatgtcaat cactcgcggc tggttagtct gattggagag gcattcggtg
      961 gttgcgaagg agttggcagc cgaccacacc agatttcgac ctccattcca catccgtctg
     1021 ccccgatcct tatcgaacag aagaacgagc ttgaacaggc acatcttgtg attgcttcgc
     1081 cctggccgga tgcgttgagt gacaaccgtt acgccgccag tctccttgcc tccgttattg
     1141 gaggaggaac ttccagtcgc ttgtggcaga aagttagaga ggaacgaggc ctggcttact
     1201 ctgtaggcgc gggtggcagc gcgttcaccg acgttggcgt cttcaacgtt tacgccggaa
     1261 cctcaccaga acaattggac gaagttttgg acctggcttt gattgaattg cgacgagttg
     1321 tgcaagactg tgtttcggaa gaagaacttc gcatcgccaa ggagcaagca atctcttcaa
     1381 ttctactcgg cctcgaatcg tcgagcgctc gagccagcac cctcgctcgg caggaaatcg
     1441 ttcatggacg acgaatttct cctgatgagg tgatagaaag aattcgtgca accagatccg
     1501 atgagttacg cgaggttgcc cgaacttatt tcaggtctga agcgatttcg ctcggagccc
     1561 tcggaaatct caacggcttt agagtcgatc gctctcgtct ttcaatgtag ctccatcact
     1621 tcctgctgaa gattgcaaaa ccgcttatag ccgttttctc ttataagtgg taaattcact
     1681 tcgccttctt ctatcagtgc agagctagcc tttctgatga aacttaccgt acttggcagc
     1741 ggatcatctg gcaatgcggt tttgatcgtt gccggaaata ctcgagtcct tgttgacgcc
     1801 ggactcagcg ccaaagaaat cacacgcaga ctttcacttg ttggcgaaga tgtgcaacaa
     1861 ctcgacgccg tccttgtaac tcacgagcat ggcgatcatg ccggcggtct aagggtgttg
     1921 cttcaatcag ttgattgccc ggtctacatc tcggctcaaa cgcttgatgc ttacgttagc
     1981 gagcgcttta acgtcaacaa tgaagaacct aagagacgcg cgaaggcttt gagtaatcgc
     2041 gtcgaacaaa ttgagtccag ccgcgacttc cgcatcggcg agatcgactt tcatcctttc
     2101 acgatccctc acgacgccgt agataatttt ggatttaccg caacgcatca gggagtgaag
     2161 attgcgaccg ttatggactt cggccacgtc acaactctca tgacagagcg ccttcgtggc
     2221 tgtgcggcca ttgtcatcga atcgaaccac agcagagaca tgctcaaggc ttgtgattcc
     2281 tatccatggg agttgaagca acgcatc
//
LOCUS       DASSFP010000293         5955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1854304, whole genome shotgun sequence.
ACCESSION   DASSFP010000293 DASSFP010000000
VERSION     DASSFP010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854304"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1332)
                     /gene="larA"
                     /locus_tag="VFH91_06500"
     CDS             complement(64..1332)
                     /gene="larA"
                     /locus_tag="VFH91_06500"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HET6862676.1"
                     /translation="MSDIQLRYGDASFNFEYDPNRFEVLNGDGPVRALADIEIGTAFD
                     SPIGGPHFDEAIDKDDSVLIVVSDATRATASAQIVNLFVRRLIQAGVSPANLAIIFAT
                     GIHRPVTHAEKADLITPFILQRVRTIDHDAYDQRNLVQLGVTSRGTPVTVNKALQEFP
                     RIVVVGGINFHYFAGFTGGRKSICPGLAAAETIEATHLLALDFKQGGRRAGVGPGLLA
                     GNAVNEECEEIAAHVAPTFAINAIVGDDKTAVAVFCGDWRQAHHAACEQYLKNRSRLI
                     SGKRDLVIVSCGGSPHDINLIQAHKAIEMASYACNEGGSIILLAQCRDGLGRANFLQW
                     FDSQDSRSLENRLKDHYEVNGQTAWSLFEKTERFNIYLISDLAVQDVHHMRMHPSPNL
                     SGALQEIGPYKSGYVMPRGAAILPRISTGM"
     gene            complement(1360..1602)
                     /locus_tag="VFH91_06505"
     CDS             complement(1360..1602)
                     /locus_tag="VFH91_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862677.1"
                     /translation="MELSLLITLDLREQAALQAALVTHGAPDALVTLALTGACRIVSI
                     EEARMVRKWLAEARTTGEADFSSLHVIEKAMVEFGL"
     gene            complement(1638..2588)
                     /locus_tag="VFH91_06510"
     CDS             complement(1638..2588)
                     /locus_tag="VFH91_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6862678.1"
                     /translation="MEQALDSNRHSPRHQHSAESWRRLSVVLCLTAFYMVAEAAGGWW
                     TGSLALLADAGHMFTDVAALALALTAVWFSARPATSSKTFGYYRLEIIAALVNGVALV
                     VISLLIFYGAWERWLAPPVVRGGAMMIFAAGGLVVNLVCAWILHAHDQVDLNMRGVWL
                     HVIGDALGSVAALAAGACMWGFGWYTADPLFSVLIGALIIWGSIRLIRESTNVLLEGT
                     PAHINLAAVENAILNTNGVDDVHDLHVWTITSGREALSAHVIHSHTISQPDLLKELRI
                     KLHDKFGVDHLTIQMETPDFEDEMFHFCHAGTACFRSDRN"
     gene            complement(2824..3468)
                     /locus_tag="VFH91_06515"
     CDS             complement(2824..3468)
                     /locus_tag="VFH91_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862679.1"
                     /translation="MSATSSSPASDQSALSASPSTDHPRYDRPTAQAFQLATEKPAQD
                     VMASDAEEALRALLGILNKGGEGSAHTSRIKRKILVCTAESYREQIARKLTEDGYQVY
                     IAEDTRQAVETMRANQMEIVLLDPQFDPAEQGALFVVREINILRPPHRRRVFFVLLSP
                     SLRTLDAHAAFLNNSNAVINIQDLNDIARILEVGLREFNELYRELNGALHLAAL"
     gene            complement(3570..4757)
                     /locus_tag="VFH91_06520"
     CDS             complement(3570..4757)
                     /locus_tag="VFH91_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET6862680.1"
                     /translation="MSFFDLPPVIERMLLVSDKISDLNFSVGQPPQVEINGKLTPVQP
                     LGMQKLTPYQTEIIAMTLLHGNADAAERLVRTGTADLSYSLPSRARFRVNIFQQRGVY
                     SIVMRVIPTDIPTLKSLGLPMHLSEIAELRNGLVLVTGPTGSGKSSTQAAIIDIINEK
                     KHYHIVTIEDPIEYLHLHKCSTINQREVGHDTRDFPSALRATLRQAPKVILIGEMRDF
                     ETTEIALEAAETGHLVLSTLHTIDASKTVDRIIGLYPKNEEQVIRTRLAQTFRYIVSQ
                     RLIPRSDGRGRVPVVEILRSSPRTREYIEAGEVEGKSLLDAMRDGKLDGMQDFDGVIK
                     QLIEAKMISFEDGLAFATNQNNLLLSLQGMTAAEDFIRRDGGPVPISDNLNDSGSMLG
                     LIE"
     gene            complement(4892..5530)
                     /gene="thiE"
                     /locus_tag="VFH91_06525"
     CDS             complement(4892..5530)
                     /gene="thiE"
                     /locus_tag="VFH91_06525"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6862681.1"
                     /translation="MRSLFQIPPLYPLTDTELSGLSHAEQVRRLAAGGATFIQLREKR
                     MSGRSFYEEAKQALEVGREFGAKILINDRVDICLALQADGVHLGQDDIHPVEARRLLG
                     DHALIGYSTHNVKQALESMVFPVDYLAVGPIFGTTSKSKPDPEVGIEGLRQVKAVVGD
                     IPLVAIGGITPSLAQSVLQAGADSVAMISALLVPSDGLTQRTQQILQALTPA"
     gene            complement(5532..>5955)
                     /locus_tag="VFH91_06530"
     CDS             complement(5532..>5955)
                     /locus_tag="VFH91_06530"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET6862682.1"
                     /translation="LLNTMFLRWGPWQGPMRGRATGSLVADRTGDTTTYALFNLQERG
                     TLFVRPGIRVYEGMIIGENARAVDLDVNAIKEKKLTNMRASSADDAMRLVPPKDLSLE
                     QALEFIADDELVEVTPQTIRLRKRILAANLRPKRKESE"
BASE COUNT         1478 a         1585 c         1492 g         1400 t
ORIGIN      
        1 gtcgctccac ttccaactgc gaacacttgc gactgtcagc ttgaggtaca ttactgttcc
       61 ttatcacatt ccagtcgaaa tacgcggcag gatcgcagcg cctcgcggca taacatagcc
      121 gctcttatat ggaccaatct cttgcagcgc tccagacagg tttggagaag ggtgcattct
      181 catatggtga acatcctgta ctgcgagatc cgaaatcaaa taaatgttga accgttctgt
      241 tttctcgaac aacgaccagg cagtttggcc gttgacttca taatgatctt tcagacggtt
      301 ttccagtgag cgggaatcct gagaatcgaa ccattgcaaa aagttagcgc gcccgagacc
      361 atcgcgacat tgggccagca agatgatcga ccctccctca ttacacgcgt acgaggccat
      421 ctctatcgcc ttatgagctt gaatgagatt gatgtcgtgc ggagagcctc cgcaactgac
      481 gatcactaag tctctcttcc cgctaatcag tctcgaacgg ttcttgagat attgctcaca
      541 cgcagcatgg tgagcctgac gccagtctcc gcaaaaaact gccacggcgg tcttgtcatc
      601 accaacaatt gcgttgatag cgaaggttgg tgcgacatga gcagcgatct cctcacactc
      661 ttcgtttact gcgtttcctg ctaacagtcc cggtccaaca ccggctcgtc gtccaccctg
      721 tttaaagtcc aatgcgagaa ggtgagttgc ctcgatagtt tcagctgctg cgagacccgg
      781 acaaatcgat tttcgtccac cggtaaaacc ggcaaagtaa tgaaagttga tgccaccaac
      841 gacaacaatg cgcgggaact cttgcagtgc tttgttaacc gtaactggcg tgcctcttga
      901 ggttaccccc aactgcacta gatttctttg gtcgtacgcg tcgtgatcga tcgttctgac
      961 tcgttgcaga atgaagggag tgatcaggtc agccttttcc gcgtgtgtta caggacgatg
     1021 gattccggtt gcgaagatga tcgccaaatt ggctggagag acgccagctt gaatcaaacg
     1081 tcgtacgaaa aggttgacta tttgagcgct tgcggtagct cgggtagcgt ccgacaccac
     1141 tatcaggacg gagtcgtcct tatcgatggc ctcatcgaag tgtggaccac caattggaga
     1201 atcgaaggcg gtaccgattt caatgtcggc aagagcgcgg actgggccat caccattcaa
     1261 tacttcgaaa cgatttggat cgtattcaaa gttgaatgaa gcgtctccgt aacgcagttg
     1321 aatatcgctc atagaggacc gagactcttt tagtcgaaac tataaaccaa attccaccat
     1381 cgccttttcg atcacatgaa gcgacgaaaa atcggcctcg cccgtggtgc gagcctcagc
     1441 cagccatttc cgaaccatcc gcgcttcttc aatcgaaaca attcgacagg cgccggtaag
     1501 ggccaacgta accaaagcgt ccggcgctcc atgggtgacg agtgcggctt gaagcgcggc
     1561 ctgttctctc aggtcaagcg taatcagaag gctgagttcc atcggctgtg gtggaaaaac
     1621 ctgccaactt tttaatttca gtttctatca gaccggaagc acgccgtgcc ggcatggcaa
     1681 aagtgaaaca tctcatcttc gaaatcaggc gtttccattt ggatcgtgag atgatcaact
     1741 ccaaacttgt cgtgcaactt aatgcgcaac tctttaagta gatcgggctg cgaaatagta
     1801 tgcgagtgaa tgacatgcgc actgagtgct tcacgccctg aagtaatcgt ccacacgtgc
     1861 aggtcgtgaa catcatcgac cccattcgta ttcagaattg cattttcaac tgccgccaga
     1921 tttatgtgag ccggtgttcc ttccaaaagt acgttggtcg actcgcgaat cagtctaatt
     1981 gatccccaga ttatcagggc acctatgagg acgctgaaaa gtgggtccgc ggtgtaccaa
     2041 ccaaagcccc acatacaagc ccccgccgct aaagcagcga ctgatccgag cgcatctccg
     2101 atcacatgga gccacacccc tctcatattc agatcaactt gatcgtgggc gtgcaggatc
     2161 caggcacaaa ccaggtttac tacgagacca ccggccgcaa aaatcatcat tgcaccaccg
     2221 cgaacaactg gcggtgcaag ccaacgttcc caggctccat aaaaaatcag aagcgaaatg
     2281 acgaccagtg cgacaccgtt taccagcgcg gcaataattt caagtctgta ataaccaaaa
     2341 gtcttgctgg acgtcgcggg gcgcgcacta aaccaaacag ctgttaaggc aagggccaaa
     2401 gccgcaacat ccgtaaacat gtgacctgca tctgcaagta acgcaagaga gccggtccac
     2461 cagccacctg cagcctcggc caccatataa aaagccgtaa ggcatagcac tacactcagc
     2521 ctgcgccaac tctccgctga gtgctgatgc ctgggtgaat gcctgttgct atctaaagct
     2581 tgttccacgc ttgtctccga tcccgtttcc ggaacgaact gttcggaaga gattcaatgg
     2641 ggacgcgcgc taagattagc aaattctctg tcggcactag tagctctgta aatgttcggt
     2701 cggcctgatc cggtgtcgcg tccctcccgt gccgcccttt ctttaccgca gatgcgtcaa
     2761 ggaaaacgag gaaggggccc agagtggccc cttccaaaag tcgcaacttg acaggatcgc
     2821 aaactacagc gcggcaagat gaagcgctcc atttaattca cgatacagtt cgttgaattc
     2881 acgcagaccg acctcgagaa tgcgagcaat gtcattcaag tcctggatat tgattaccgc
     2941 attgctgtta ttcagaaagg ctgcgtgcgc atcaagtgtg cgcagagagg gcgagagcag
     3001 tacgaagaac actcttctac gatgaggggg ccgcaggata ttgatctccc ggacaacgaa
     3061 caacgctccc tgctccgccg gatcaaactg aggatccaga agcacaatct ccatctggtt
     3121 tgcgcgcatc gtttcgacgg cctggcgggt atcctcggcg atatacacct gatagccatc
     3181 ctcagtgagc ttgcgagcaa tctgctccct gtaactctct gctgtgcaca ccagaatctt
     3241 ccgtttaatc cgagaagtgt gagctgaacc ttcaccaccc ttattcagaa tcccaagcaa
     3301 cgcacgcaac gcctcttcag cgtcagaggc catcacatcc tgcgccggtt tctccgtggc
     3361 caactggaaa gcctgcgcgg ttggccggtc gtaacgagga tgatcagtcg aaggtgaagc
     3421 gctcagggca ctttgatccg aggccggact ggaagacgtt gcactcactg tgttgttaca
     3481 tttcggacaa cgcagagtga cgtttctcga gggagctttc tcctcatcga actgtaggcg
     3541 cgtggagcaa ttttgacaga caatgatcgt cattctatta accctaacat cgaccctgaa
     3601 tcattaagat tgtccgagat tggaaccggg ccaccatcac ggcggataaa gtcttcagct
     3661 gccgtcatgc cttgcagcga aagtagtaga ttattttgat tggtagcaaa ggctaaccca
     3721 tcttcaaaac taatcatctt tgcttcaata agttgcttga taacaccgtc gaagtcctgc
     3781 attccgtcga gcttgccatc acgcattgcg tcgagcagcg acttgccttc gacttcgccc
     3841 gcttcaatat actcacgcgt tcgtggactt gaccgaagta tctcgacgac tgggactcga
     3901 cctcttccgt cagaacgcgg aatcaaacgc tgagacacga tgtagcggaa agtttgagcc
     3961 agtcgagttc tgatgacttg ttcttcattt ttgggataca gaccaataat ccggtcgacc
     4021 gttttcgaag cgtcaatggt gtgaagcgtt gagagtacga ggtgccccgt ttcggcggcc
     4081 tctaacgcga tttcagttgt ttcaaaatca cgcatctcac cgatcagaat tactttgggc
     4141 gcctgacgaa gagttgcccg taaagccgat gggaagtctc tcgtatcgtg tccaacttca
     4201 cgttgattga tagtcgaaca tttgtgcagg tgaagatatt cgattggatc ttcaatagtg
     4261 acgatgtggt aatgcttctt ctcgttgatg atgtcgatga ttgccgcctg ggtcgaactc
     4321 tttcctgaac cggttggacc agtaaccaac acgagtccat ttctgagttc ggcaatctcc
     4381 gataaatgca taggtaaccc caacgacttc aaagtgggaa tgtcggtagg aataactcgc
     4441 atcacgattg aatagacacc gcgctgctga aagatgttga ctcggaagcg tgctcgcgag
     4501 ggcagcgaat agctcaaatc tgcggtgccg gtccgcacca ggcgctcggc cgcatcggca
     4561 tttccgtgca gcagagtcat cgcgatgatc tcagtctgat agggggtcag cttttgcatt
     4621 cccaacggct gaacaggagt gagcttaccg tttatttcga cttgaggcgg ttggccgacg
     4681 gagaaattga ggtcgctgat cttgtcggag acgagcagca tgcgctcaat tacgggcggc
     4741 agatcgaaga aactcattaa tttaggaact ccggaaaacg atagtcgatg aaggaatgcg
     4801 ccaggaatgg aaaggctgag gagctcggaa cgccctcaag cgccatcgag attttcgcgc
     4861 ataaacagaa ttaacgcaag caaaatatgt tttacgccgg ggttaaggcc tgcagaattt
     4921 gctgagtgcg ttgggttaag ccgtctgaag ggactaaaag agcggagatc attgcgacgg
     4981 aatccgcgcc agcctgaagg acgctctgtg ccaatgaagg agtgatgccg ccaatcgcta
     5041 ccagtggaat gtccccgaca acggctttaa cctgtcgcaa gccctcaatc ccaacttccg
     5101 ggtcgggttt actcttgctg gtggtgccga agattgggcc gacggcaagg taatcgacag
     5161 gaaacaccat cgactccaaa gcctgtttaa cgttatgagt tgagtacccg ataagcgcat
     5221 gatcaccgag gagccttctg gcttcgacgg gatgaatgtc atcttggccc agatgtacac
     5281 catcagcttg cagggcaaga caaatatcaa ctcgatcgtt gatcagtatc ttcgccccaa
     5341 actctcgacc aacctccagt gcttgctttg cttcctcgta aaaactgcga ccgctcattc
     5401 ttttctcgcg aagttgaatg aaggtcgcgc cgcctgctgc aagtcgtctg acttgctccg
     5461 catgtgacaa tcctgaaagt tcggtatctg taagcgggta aagaggaggt atctgaaaca
     5521 gacttcgcat tctattccga ctctttacgc ttcggacgta ggtttgccgc gagaatgcgt
     5581 ttccttaacc tgattgtctg cggtgtaact tctaccagct cgtcgtcggc aatgaattcc
     5641 aaagcctgtt ccagggaaag gtctttaggt ggcacaagtc gcatggcgtc gtcagccgag
     5701 gaggctctca tgttggtaag tttcttttcc ttaatcgcat taacgtcgag atcaactgcg
     5761 cgagcgttct cgccaataat catgccttcg taaacacgaa tcccgggtcg aacgaacaga
     5821 gttcctcgct cttgcaggtt gaaaagcgcg taagttgtcg tatcacccgt ccggtcggca
     5881 accaatgacc cggtagcgcg tcctcgcatc ggaccttgcc acggccccca acgtaaaaac
     5941 atcgtattca gtaat
//
LOCUS       DASSFP010000294         5466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1113228, whole genome shotgun sequence.
ACCESSION   DASSFP010000294 DASSFP010000000
VERSION     DASSFP010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113228"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFH91_06535"
     CDS             complement(<1..528)
                     /locus_tag="VFH91_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862683.1"
                     /translation="MQNALAESTIPYTMSATERRVETTESRRTLIILVAIAAAVVIAL
                     FFYWLMRAGSGPVGGDVSLQGAIRPGSAEFDQYKDKIVLDTPEADEAKRALGDIVMSL
                     HTTVRNFTGKTLSGLEVRAAVVDHQDKAVKERTVVVIPTRQRELGPNKTMTVSVMLEG
                     MTDTDDRANIRMQVTG"
     gene            complement(611..991)
                     /locus_tag="VFH91_06540"
     CDS             complement(611..991)
                     /locus_tag="VFH91_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6862684.1"
                     /translation="MTSEKPAKVLLVEDTEDNRFMMRRLLEMTGYEVVEARNGEEAVK
                     VAQTELPDLILMDLSLPIIDGLAATRLIREIEALKKTPIVTVSAHDTQDFQNEALRAG
                     SDGYITKPIDYSELEQLIVKLLNR"
     gene            1209..1883
                     /locus_tag="VFH91_06545"
     CDS             1209..1883
                     /locus_tag="VFH91_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862685.1"
                     /translation="MSRTEDTSTLQDLGRASIQIVHDLKNQLNGLKLYATFLRKRIEK
                     GDRPEDEQETIGKLINGLDRAASDLSTIVEYGREIELRTQSGFDLQKILRTVCAELSS
                     ANSLVTCKIEADSLLGEFDSSKLADAFRWISVAALKHLQNTQDSGQIIITAQLEAENG
                     RQAVITWSGLAALEYDPFVSFSGSEEIRLSLAAKIIESHAGSAKYVNKTFVVRLPLIN
                     QGDKLK"
     gene            1947..3338
                     /locus_tag="VFH91_06550"
     CDS             1947..3338
                     /locus_tag="VFH91_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862686.1"
                     /translation="MDNKEVRILVVDDEPMMADSLRQNFMEEGYSVDTAPTGAQAIEL
                     FDQGGHQLAICDLQLPDMDGLEVLRHMKDARPTTEVIVVTGYGSVQKAVEATKAGAFY
                     FVEKPFDFEELQPLVEKALERRELVAETTNMRRQLSTRAEYFNIIGASKPMQTIYETI
                     ESVAKSDANVLIVGESGTGKELIANAIHYQSLRSKKPFIKVNCAALPKELIESELFGH
                     TKGAFTGAHADKEGLVQHAAGGSLMLDEIAEMPVELQPKLLRVLQERSYRKIGSEKTY
                     AVDFRLVCSTNRPPADAIRDGLLRDDLFYRISTITIHVPPLRERNEDIQLLTEHFLHM
                     YAQKYERQINGVSQAAYQRLFGHSWPGNVRELQNVIERAVLLAKDKFIEPIDLPFDNG
                     SLPEGTAAQNSAWDIPPNMTLEDIERLVIEKTLQRTGGNKQAAANLLGIYRPRLYSKI
                     RKYNIDVTSLVSA"
     gene            3407..4732
                     /locus_tag="VFH91_06555"
     CDS             3407..4732
                     /locus_tag="VFH91_06555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6862687.1"
                     /translation="MDDLVESNAAQMNIVPQNAKPALRLRSIDALRGAAALGVVLYHS
                     VDRSPAVVPAGWSRYPEILLQFVSSFGYIGVFLFFVISGFCIHLQWARAKANGEQPVI
                     SFGSFWKRRVRRLYPPYIIALAIYLGLTAASVGIDVTRFFIYDVGMHLFMLHNLDPKT
                     CYSINGVFWTLAIEEQLYLAYFLLIFVRARFGWITTLVVCLLARAGWMVFSHVIWLRT
                     GFGIPVPEAAASHWFTWALGAISVEAMFGLIQLPKWCSNLKLAGVLIIGAAALSNALP
                     WIPKDTIWHNGSWFLLHPLWGVGFLIVVNRCVVSEQRWIRSSQLPLLVSLFATLGVFS
                     YSIYLTHELTIMQSWRWTSATSSQVGKVLLLVMPATIVAAWVFFWFCERPFIVKRKAG
                     QEAPVLIGESHAEESLVGLFPKRFRPVPNERSQTAEAIKPQAIETVVEA"
     gene            complement(4782..5153)
                     /gene="queD"
                     /locus_tag="VFH91_06560"
     CDS             complement(4782..5153)
                     /gene="queD"
                     /locus_tag="VFH91_06560"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HET6862688.1"
                     /translation="MFEVMIERNFSSAHQLRGYKGKCENLHGHNYKIEIYARGRELDN
                     IGLLVDFVELKAAADEVVTYLDHRNINELPPFDELQPSAENLAKYVLERVAKRVGDER
                     VQIYKVRCFETPTSVATYQLD"
BASE COUNT         1353 a         1323 c         1356 g         1434 t
ORIGIN      
        1 accagttacc tgcatgcgga tgttggcgcg atcgtcagta tcagtcatac cctctaacat
       61 taccgacaca gtcatagtct tgtttggacc taactcacgc tgtcgcgtcg gaataaccac
      121 gacagttcgc tccttcaccg ctttgtcctg atggtcaacg acggccgctc tgacttctag
      181 tccacttaag gtcttaccag taaaatttcg gaccgtcgtg tgcagactca tgacgatgtc
      241 acccagggcg cgcttagcct cgtcggcttc gggtgtgtcg agaacgatct tatctttgta
      301 ctgatcgaat tcggctgaac cgggccggat ggctccctgc aagctgacgt caccgccgac
      361 cggaccactc cctgctctca tcagccaata aaaaaagaga gctataacca cggcggcggc
      421 aatcgccacg agaattatca aggtccggcg tgactcggtt gtttcaacac gcctttctgt
      481 agcactcata gtgtagggaa ttgtagattc cgctaaagcg ttttgcaaag agttgtggtc
      541 cgttgtctcc atataaagga cttagacaca tctcggctgc gttagttcca attccaaaac
      601 caacgaaatt tcagcggttg agtaatttaa ctattaattg ttcgagttct gaatagtcga
      661 ttggctttgt gatgtaacca tcagagccgg cccggagcgc ctcgttctga aaatcctgcg
      721 tatcatgtgc tgatactgtc actatcggcg ttttcttaag ggcttcgatc tcgcggatca
      781 atctcgtagc ggccagccca tcgatgattg gcaggctcaa atccatcaat atcaagtctg
      841 ggagctctgt ttgggcgact ttaaccgcct cttcgccatt tcgcgcctcg acgacctcat
      901 agccggtcat ttcgagaagc ctgcgcatca tgaaccgatt atcttccgtg tcctcgacga
      961 gcagaacctt cgccggcttt tcgctagtca ttttttcttt tcccagggca atcaagcccg
     1021 ccaagttgtt gattttactg tatcactaga atacgcggac gttttttgat aacatcccgc
     1081 actgaaacac gacacccatt gagtaacgct agaagtctaa ttcgtacact caacgaaggt
     1141 agtcgagttc cctttcccct tagcgacccc gaagcgatta agagagattt gcgctcatga
     1201 agcccttaat gtcgaggacc gaagatacaa gtacgcttca agacctcgga cgagcctcta
     1261 ttcagattgt ccatgacctt aagaaccaac ttaacggtct caaactctat gccacgttcc
     1321 tgcgcaagag gattgaaaag ggggataggc cagaagatga gcaggagaca atcggaaagc
     1381 tgataaatgg cctcgatcga gccgccagtg atctctcgac gattgtggaa tatggtcgcg
     1441 aaattgaact tagaacacag tcgggatttg acctgcagaa aatcctccga acagtttgtg
     1501 ccgagctaag tagtgctaat tccctggtga cttgcaagat agaagctgat tcacttttgg
     1561 gagaatttga ttcgtcaaag ttggccgacg catttcgatg gatttcagtg gccgcgctca
     1621 aacacctgca gaacacgcag gattcgggac aaataataat aacggcacag ctagaggctg
     1681 aaaatggcag acaagctgta atcacctggt ctggcctggc agccttggag tatgacccat
     1741 ttgtatcttt ttctggcagc gaagaaattc gtctctccct cgctgcgaag atcatcgagt
     1801 ctcatgctgg atcagctaaa tacgttaaca agactttcgt ggtcaggctt ccactcatta
     1861 accagggtga caagctaaag tgaaagagat tgggagattg gattatgagc cctcaaggtg
     1921 ctgcgacttc gagagaggag agaaaggtgg ataacaaaga ggtacggata ctggtggtgg
     1981 atgacgagcc aatgatggcc gattccctaa gacagaattt tatggaggaa ggctattcgg
     2041 tcgataccgc tccaaccggt gcccaggcga tagagctttt tgatcagggt ggccatcaac
     2101 tggcgatatg cgacttgcag ttgcccgata tggatggact cgaagttttg aggcatatga
     2161 aagacgccag accaacaact gaagtcatcg tcgtaaccgg ttatggatca gtccagaaag
     2221 ccgtcgaagc aacgaaggca ggtgcattct attttgtaga aaagcccttc gactttgagg
     2281 agttgcagcc actggttgag aaggcgttgg aacgtcgtga actcgtcgcg gaaacaacaa
     2341 acatgcggcg tcagctttct accagagccg agtacttcaa tatcatcggc gcttcgaaac
     2401 cgatgcaaac gatatacgag acaattgagt ctgtagctaa gtcagatgca aacgttctta
     2461 tcgtgggcga gtcgggaacg ggtaaggagt tgattgcaaa cgcaattcac taccagtcgc
     2521 tccgttcgaa gaaacctttt attaaagtta attgcgctgc attgccaaaa gagttgatcg
     2581 agtcggaatt gtttggccat accaagggtg cttttaccgg ggctcatgcc gacaaggaag
     2641 gactcgtaca gcacgccgct ggtggctcgt tgatgctcga cgaaatcgca gaaatgccag
     2701 tcgagcttca gccaaagttg ttgagagtct tacaagaacg cagttatcgc aaaatcggat
     2761 ccgaaaagac atacgcggta gattttcggc tagtctgttc aactaatcgt ccgcctgcag
     2821 atgcgatcag ggatggtctg ctccgagacg atctgtttta tcgtatttct actatcacga
     2881 ttcacgttcc acccttgcgt gagcgcaatg aagatattca gcttttaact gagcacttcc
     2941 tccatatgta cgctcaaaaa tatgagcggc aaattaacgg tgtttctcaa gctgcttacc
     3001 aacgactgtt tggacactca tggccaggca acgtgcgtga attgcagaac gtgattgaac
     3061 gcgcggtatt gctggcgaaa gacaaattca tcgagccaat cgatttgcct ttcgacaatg
     3121 gctcgctccc tgagggaacc gcagcgcaaa attctgcgtg ggacattccg cctaatatga
     3181 ctctggaaga cattgagcgt ttggtgatcg aaaagacttt gcagcgaaca ggcggaaaca
     3241 aacaggcggc agcgaatctt ttgggaatct accgcccacg cctttacagc aaaattcgaa
     3301 aatacaatat cgacgtaacc tctctggttt ctgcatagag agaaaacgtg taaattgacg
     3361 ttttaactaa agtggcctga ccatcgggcc actttctttt gtttctttgg acgacctcgt
     3421 agaatcaaac gccgcccaga tgaacattgt cccgcagaac gcaaaaccag cgctcagact
     3481 gagaagcatc gacgcactcc gtggcgcagc agctttaggg gttgtgctct atcattcagt
     3541 ggatcgaagt cccgcagttg ttccggccgg atggtccaga tatccggaaa tactgttgca
     3601 gttcgtaagc tcgtttggct acatcggagt ctttctgttt tttgtgatct ccggattctg
     3661 catacacctt caatgggctc gagcaaaagc aaacggagaa cagccagtca tttcgtttgg
     3721 atctttctgg aagcgtcgag ttcgtcgact ttacccgcca tacattatcg ctcttgccat
     3781 ctatctcgga ttgaccgctg cttcggttgg tatcgatgtt acgcgctttt ttatttacga
     3841 cgtcggtatg caccttttca tgttgcacaa tcttgatcca aagacctgtt acagcatcaa
     3901 tggcgttttt tggactttag cgatcgagga acaactctac cttgcgtatt ttctattgat
     3961 ctttgttaga gcaagattcg gatggattac gacattggtt gtttgtttgc tggctcgtgc
     4021 gggttggatg gtcttcagcc atgtgatttg gcttagaact ggttttggca ttcctgtacc
     4081 cgaagccgct gcctcacatt ggtttacatg ggcgctaggt gcgatctcag tcgaagcgat
     4141 gtttgggttg attcaactac caaagtggtg ttcgaatcta aaacttgccg gcgttctcat
     4201 catcggagcc gccgcactgt caaacgcgct cccatggata cccaaggaca ccatctggca
     4261 taacggcagt tggtttctat tgcatccact gtggggtgtt ggattcctga tcgtcgttaa
     4321 ccggtgcgtt gtttcggaac aacgttggat tcggtcctct caacttccgc ttcttgtttc
     4381 gttattcgca acactcggag ttttctcata ttcaatttat ctaacgcatg agctgacaat
     4441 catgcagtcg tggcgttgga ccagtgcgac ttcctctcag gtggggaaag tgctcttgtt
     4501 ggtgatgccg gcgaccattg ttgctgcctg ggtcttcttc tggttttgcg agaggccgtt
     4561 tattgtaaag cgcaaagccg ggcaggaagc accagtcctt attggcgaat cacacgccga
     4621 ggaaagcctg gttgggttgt tccccaaacg gtttcgcccg gttccgaatg agcgcagcca
     4681 gaccgccgaa gcaatcaagc cgcaagctat agagacggtt gttgaagctt gacgaaatag
     4741 cccggcggac gtttggaggt cagcgccaaa atcccaccct tttaatctag ttggtaggta
     4801 gcaacactgg taggtgtttc aaaacatcga accttgtaga tctgtacacg ctcatcgcca
     4861 actcttttag caactcgctc gagcacatat tttgcgaggt tctcggcaga aggctgaagt
     4921 tcatcgaatg gtggtagttc gttgatgttt ctatggtcca gataggttac tacctcatcg
     4981 gcagcggcct tcaactcgac aaagtccacc agtaagccga tattatcgag ctcacggcca
     5041 cgagcataga tttcgatctt gtaattatgc ccgtgcagat tctcacattt tcctttgtaa
     5101 cctctgagct gatgcgcgct cgagaagttg cgttcaatca ttacttcaaa catatagggt
     5161 gtgctgcgct ttgcgctttg ttttttgtgc tttgtacttt gtccgctgtc agatgtccgt
     5221 attagtcgat tcaattcgat ttgagataca gagttcaaat cgaagtacaa aggtacaaaa
     5281 aacaaagcac aaagtacaaa gtacccctga ccacggacaa tctagataaa catcgcggct
     5341 agatcatcaa tgcgtgacgt ggtgcggcaa ggtggcaagc tgcgaaggaa agtttgtcca
     5401 taggctttag tgcggagccg cggatccaga accgcgagca ctccacggtc gcttgtactt
     5461 cgaatc
//
LOCUS       DASSFP010000295         4697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_675016, whole genome shotgun sequence.
ACCESSION   DASSFP010000295 DASSFP010000000
VERSION     DASSFP010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675016"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VFH91_06565"
     CDS             complement(<1..1499)
                     /locus_tag="VFH91_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET6862689.1"
                     /translation="MSGIVGIITFGGQPLDKQLLRTITESMRFRGPDACEILSSANFG
                     FGHTLLRTSSESTGEKQPLNLNQKVWLTADARIDGRAELIAKLSELNAREERSHEDPS
                     DAELILRAYLAWGEECVAHLIGDFSFAILDLEKQRLFCARDHFGIKPFYYARSGKSFI
                     FSNPIETIQLHPEVTARLNDQAIGDLLLFDFNRDPNRTSFQDISRLPPAHTLTLDLTT
                     GECRIKRYWSSPEAVTLRYRNQSEYLEHFQDLFTRAVEDRIRTNRLQSFLSGGLDSTG
                     IAREVVRIRQRLNRDLSLTGQCVVYRELMDDQEGHFASIAAEALGIPLYYVVADPYLL
                     YEDAGAPELLKPEPVHEPLQKLTYHQFSKIQADCRVALSGLGPDATMSFWLHSHIASM
                     KSNSRLAELTMDLIRYFWLKRRPPRLHVLDRLGRTPGPQTLSDTYPRWLNKKFEERLE
                     LNNREVLLQSKEMGSHFRRKQAFQLLSDPGNWTYHFEYADSGTTRLPVEV"
     gene            complement(1579..1863)
                     /locus_tag="VFH91_06570"
     CDS             complement(1579..1863)
                     /locus_tag="VFH91_06570"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET6862690.1"
                     /translation="MPVSFEDRVKLPDDVLISSLQEESVILNLDTERYFGLDDVGTRM
                     LSVLTTSTSIEAAYNLLREEYEVDDDVLKEDLLALADQLVEQGLIEISNQ"
     gene            2159..3661
                     /locus_tag="VFH91_06575"
     CDS             2159..3661
                     /locus_tag="VFH91_06575"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6862691.1"
                     /translation="MKLKLTDIHKSFGPTVALDGVSFDVSEGEVHALIGENGAGKSTL
                     MNLIAGALQPDKGEMTIDAKKYSPGSTLESRLNGVALIHQELSLCPHLTVAENILMGM
                     EPARAGWLDRNQMMKRTSEVLQTFHHSDIRPDTRVGNLSVAARQIVEISRALAAEARI
                     ILMDEPTSSLQREDVVQLFALIRKLSSEGIAVVYISHFLEEVRQIADRFTVLRDGRSV
                     ATGEISTISDDELIAKMVGRTQKTLFPVRDRPTEKSDIALEVTNLSSPPLLKNASFQL
                     RRGEILGIAGLMGSGRTPLLRAIFGLQRSENGTVAVKNKKRAGGHNSPAVRLIQGLGY
                     LSEDRKGEGLTLNLSVADNTTQTKFSTCSRWGWINLAKQQKQTRRLIELLKIKARGAQ
                     QPVQSLSGGNQQKVALARLVHQDADILLLDEPTRGIDIGSKAQVYETIAECAAQQKAV
                     VIVSSYLPELFGMCDRLAVMSRGKLSIVKPIDEWTPESVLQTAIGSDADK"
     gene            3717..4610
                     /locus_tag="VFH91_06580"
     CDS             3717..4610
                     /locus_tag="VFH91_06580"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HET6862692.1"
                     /translation="MKYLSITLMPALLLSAMSNAQTVTSPLKQMVETEQAFSRTAEER
                     DTRDAFMMFIADDGLLFRPNAVNGKKWMQDHPVPPSDKHPLLAWQPAFARVAASGDLG
                     FTTGPWEFKNDKSDTTPVGYGHFVTIWKKQADGSWKFAVDLGISHPESGGPLKVWEVS
                     DEHDVKSSKVVNVSEEQKKLTAQDRAFSDQGVKRGSVKAFTSFSAPDVRLYRNNRLPF
                     VGRDASLSYLGSIKENYVWKPNQSDVSRSGDLGYTVGTYEAVPVNDPSGKVTELGNYL
                     RIWQKQNGSWRIVLDVTNPVP"
BASE COUNT         1247 a         1122 c         1178 g         1150 t
ORIGIN      
        1 acttcaacag gtaggcgagt ggtgccgctg tcggcatact caaaatgata agtccagttg
       61 cccggatcac ttaataattg aaaggcttgt ttccgtcgaa agtgacttcc catttctttt
      121 gattgcagta acacttcacg attgttcaac tctaaccgct cttcgaattt cttgtttagc
      181 caccgaggat aagtatctga aagtgtttgt ggaccaggag ttctcccgag cctgtctaaa
      241 acatgtaaac gaggtggtcg acgctttaac caaaaatagc gaatcaaatc catggtcagc
      301 tcagccaaac ggctattgct cttcattgaa gctatgtgac tgtgcagcca gaaactcatc
      361 gtggcatcag gtccaagacc tgatagagca actctacagt cggcttggat cttagaaaac
      421 tgatggtacg ttagcttctg taacggttcg tgaacgggct ccggctttaa cagttccggc
      481 gcacctgcat cttcataaag gaggtacggg tctgccacga cataatagag tggaattccc
      541 agcgcttcgg ccgcgattga agcgaagtgt ccttcctggt catccatcaa ttcacgatac
      601 acaacgcatt gcccggttag acttaaatct ctatttaacc tctgccgtat gcgcacaact
      661 tcgcgggcga ttcctgtgga gtcgaggcca ccactcagga agctttgtaa cctgttcgtc
      721 cgtatgcgat cttcgacggc acgtgtaaag agatcctgaa agtgctcgag atactccgac
      781 tgattgcggt atctcaaagt aaccgcttca ggactgctcc agtatctttt gatcctgcat
      841 tcaccagtcg taaggtcaag ggttagcgta tgagccggcg gcaaacgtga gatgtcttga
      901 aatgaggttc ggttaggatc ccgattaaag tcgaagagca acaagtcacc aatcgcctga
      961 tcgttcaaac gcgcagtgac ttctggatgg agctgtatcg tttcaatggg gttactgaaa
     1021 atgaagctct taccagatcg cgcgtaatag aagggcttaa taccgaagtg gtcccgggcg
     1081 cagaacagcc gttgtttttc aagatcaaga attgcgaatg agaagtcacc aatgagatgc
     1141 gctacgcact cctcgcccca ggctaaatat gcacggagga tgagttcggc atcgctggga
     1201 tcttcatgcg agcgttcttc acgcgcgttc aactctgaaa gtttcgcgat taactctgcg
     1261 cgtccatcta ttcgagcatc tgctgtcagc cacactttct gattaagatt taatggttgt
     1321 ttttcaccgg tagattcaga gctggttctc aggagagtgt ggccaaaacc gaagttagcc
     1381 gaactaagaa tttcacaggc atccggcccg cgaaatctca tcgattcggt tatcgtgcga
     1441 agtagttgtt tatcgagagg ctggccaccg aacgtgatga ttccgactat tccactcatt
     1501 gctctctaaa atcggaatgt tggcgtttgg tgttggacac gaatgcagcg taggtcgagc
     1561 aggtcgaaac cgtggcgact attggtttga gatctcaatc aaaccctgct cgacgagttg
     1621 gtcagcgaga gcgagtaaat cttctttcaa gacatcgtca tcgacttcat attcttctcg
     1681 gagaaggtta taagctgctt caatcgaagt tgaagtggtt agcacggaca gcattcttgt
     1741 tccaacatcg tcaagaccga aatagcgttc ggtgtcgagg tttagaatta cagactcttc
     1801 ctgtaagctt gagatcaaga cgtcgtctgg aagcttcacg cggtcctcaa aagagacagg
     1861 catcttatga actttctcct gattacgaaa ctattttggc gcgggcgata gtcgggcgac
     1921 tcaattctgt cgttgatcga tttatgtgac gaagaattga gttgagcatc gtagtgagag
     1981 accggtatca agtcaagatt atccagcaat tggtactgtt ctaatctagc cttctctgtg
     2041 taaccttggt ggcctctgtg cctcggtggt gagcaaagtc taaggtaacc atcaccacag
     2101 agacacagag atcccagaga cagcacagag agaagattag gacaccttgc tcaaaaaaat
     2161 gaaacttaag ctcacagata tacacaaatc atttggacca acagtcgcgc tcgacggtgt
     2221 tagtttcgac gtgagtgagg gtgaagtaca cgcgctgatc ggagaaaacg gagccggcaa
     2281 aagcactctg atgaacctga tcgccggcgc acttcagccc gacaaaggag aaatgacgat
     2341 cgatgcaaag aagtactctc ctggaagtac gcttgagtcg cgactcaatg gtgttgcact
     2401 cattcatcag gagttgtctt tatgtcctca cctgacagtt gcggaaaata tcctcatggg
     2461 aatggagcct gcgcgcgccg ggtggctcga tcgaaatcag atgatgaagc ggaccagtga
     2521 agttttgcag acgtttcacc actccgacat cagacctgat actcgtgtag gcaatctctc
     2581 cgtagctgcg cgtcaaatag tcgagatatc gcgagcactc gcagcagaag cccgcatcat
     2641 cctgatggac gaacccacca gcagtctaca gcgtgaggat gtcgttcaat tatttgcact
     2701 aatccgcaaa cttagttcag aaggcatcgc ggtggtctat atcagccatt tcctggaaga
     2761 agtacgacaa attgcagatc gctttactgt cctgcgtgac ggacgaagcg ttgcgacggg
     2821 agagatatcg acgatcagcg atgatgaact gatcgcaaag atggtcgggc gaactcaaaa
     2881 aacacttttt cctgtacgtg atagaccaac ggaaaaatct gacattgcac tcgaagttac
     2941 aaatctaagt tctcccccac tgctaaaaaa tgcgagcttt caactcagaa gaggtgagat
     3001 tcttggcatt gcagggttga tgggatccgg tcgcactcct ttgctccgtg caattttcgg
     3061 tcttcaacgt tccgaaaatg gaacagtcgc ggtgaaaaat aaaaagcgag ccggcggaca
     3121 taattctcca gccgtacgtc taattcaggg tttgggttac ctcagcgaag atcgaaaggg
     3181 cgaaggactt acactcaatc tttctgttgc tgataataca actcaaacaa agttctcgac
     3241 atgttctcgt tggggttgga tcaatctcgc aaagcaacag aagcagacca ggcgactaat
     3301 tgaacttttg aagatcaaag ctcgcggtgc tcaacagcca gtgcagtcgt tgtcaggtgg
     3361 taatcaacaa aaagttgcgt tggcccgatt ggtccatcag gacgcagaca ttcttttact
     3421 ggatgaacca acgcgcggca tcgacatcgg gagtaaggcc caggtttatg aaactattgc
     3481 tgagtgtgcg gctcaacaaa aagctgtcgt gatcgtaagt tcatatctgc cggagctatt
     3541 tggtatgtgc gaccggcttg cggttatgag ccgtgggaaa ttatcaatag tcaaacccat
     3601 cgatgaatgg actccagaaa gtgtcctgca aactgccata ggatctgacg cagacaagta
     3661 gcgccgctaa agttctcgcc tcgccaccgg gccaacgatg gtaaggacca agcatcatga
     3721 agtatctatc aataactctc atgccggcgc ttctgctttc agcgatgagt aatgcgcaaa
     3781 ccgtgacttc gcccttgaag caaatggttg aaactgagca ggcattctcc aggacggcag
     3841 aagaaagaga tacccgcgac gccttcatga tgtttatcgc cgatgacgga ctgttgtttc
     3901 gaccaaatgc cgtcaatggc aaaaaatgga tgcaggatca tcccgtccca ccatccgaca
     3961 agcatccgtt actggcatgg caacctgcat ttgcgcgagt cgccgcttcg ggtgatcttg
     4021 ggttcacgac tggtccctgg gaattcaaaa acgacaagtc tgacacaacg cccgtcggtt
     4081 acggacattt tgtaactatt tggaaaaagc aggcggatgg ctcatggaaa tttgcggttg
     4141 atctcggaat atctcatccc gagtcgggcg gtccactaaa ggtctgggaa gtctccgatg
     4201 agcacgatgt taagtcttca aaggtcgtca atgtttccga agagcaaaag aagctaacgg
     4261 cccaggaccg cgcgttttcc gatcagggag tgaagagagg ttcagtaaag gcgtttacct
     4321 cattttctgc acccgacgtc agactttacc gaaacaaccg ccttccgttt gtaggccgag
     4381 atgcgtcatt gagctatttg ggctcgatca aggagaatta tgtttggaaa cccaaccaaa
     4441 gcgacgtttc acggtccggc gaccttggtt ataccgtcgg aacttacgaa gcagtcccag
     4501 taaacgaccc ctccggcaaa gtaactgaat taggcaatta tctgcggatc tggcaaaaac
     4561 aaaacggctc atggcgaatt gttctggacg ttaccaatcc ggttccgtaa agttgatctg
     4621 tgagcctgag ggttacggct cgcttcgatg ggagaaccag tggcgcccga gaaccttcat
     4681 aattccctga acccaaa
//
LOCUS       DASSFP010000296         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1708808, whole genome shotgun sequence.
ACCESSION   DASSFP010000296 DASSFP010000000
VERSION     DASSFP010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708808"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFH91_06585"
     CDS             <1..779
                     /locus_tag="VFH91_06585"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET6862693.1"
                     /translation="WDELKEGETLLMQSREAFALGKVDISGKKTLVEYGFNPSSKKIS
                     VNNGWLGVDGDGDGFIDLDRFSPEAAEARDETVIFRVGAVYVSTKKVDLEKNEIIMRE
                     HGPSDYKRVELSVGSELPDFQFTDFAGKKRQLSEFRGKFVLLDFWALWCGPCRRELPY
                     QKLAYSRFQSRGFEILGLNNDPDYTQVKPTLKRNGLAWPQATMESIEPILKRYRIHYF
                     PTTLLIGPDGKIISLDQTRRKQPGLRGQELIRSLDELLPP"
     gene            complement(802..1380)
                     /locus_tag="VFH91_06590"
     CDS             complement(802..1380)
                     /locus_tag="VFH91_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862694.1"
                     /translation="MTLTQMTDEPDQEKRIARLRAELEKLGGSTHSLQSMPADLEEEF
                     LRQVLEYETAEPISLFRLLENAGLDIPVLEDLDDANVKSKLKEIIERMAALGAYLLHT
                     DHLSDRELYHYLYTDGLREEAVLFPENASYAYMIDLTGSGSDEDNQIYLKYYADEQQR
                     QRWAQDWPDDVIPDHEDPPFDRDAYLPKSPIG"
     gene            complement(1703..2389)
                     /locus_tag="VFH91_06595"
     CDS             complement(1703..2389)
                     /locus_tag="VFH91_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6862695.1"
                     /translation="MPQNSSTGARNDEPRKEGEESDEAPGIEVTATEDLMREHGILRR
                     ILLVYTESAAKLKQDAGSVPTDELEKAAQLFRVFGEDYHEKKLEEAYILPSLRQAQGA
                     ASSYVDVLIAQHVRGREITDYILSVTKGDKIPAASVEEFIKAMDSMVRMYEHHTAIED
                     TIIFPAWKAAVGDRRLDELGAKFEEIETETFGGDGFEAVQKRISEIEQNLGLSNLDMF
                     TAPPLATKKT"
     gene            2566..3021
                     /locus_tag="VFH91_06600"
     CDS             2566..3021
                     /locus_tag="VFH91_06600"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET6862696.1"
                     /translation="MDNAEIARRFYRLAALMEIRGDDPFRLRSYRNAAEAIEVWPTPL
                     QEIAEAEGETGLQTIPGIGKAIAGKVLELLSTGSFAAWDRLTAETPESVLDLTEIPGI
                     GPKTAALLHERFKVSSRADLVKFVAGGGLEMVDGIGPKTAERIKEALSE"
     gene            3110..>3673
                     /locus_tag="VFH91_06605"
     CDS             3110..>3673
                     /locus_tag="VFH91_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HET6862697.1"
                     /translation="MSKSIKRRDFMRKSAAAGIVVAAAKDNVLGFPMIMKQSAIKPVV
                     ISSANGNKFKNRGDVTAVQKAFTMITNGSDVLDAVIAGVNIVELDPLDDSVGYGGLPN
                     ADGIVQLDSSCMHGPKKRAGAVGALEGVKTPSLVAKAVLENTDHHLIVGKGAQEFARN
                     MGFKIEDDLNTDHSRKQWLEWKRRTDPS"
BASE COUNT          905 a          909 c          927 g          932 t
ORIGIN      
        1 agtgggacga acttaaggaa ggcgaaaccc tgttgatgca atccagggag gctttcgctt
       61 taggcaaggt tgatattagc ggcaagaaaa cgctcgttga atatgggttc aatccatcgt
      121 caaaaaagat cagcgtaaac aacggctggt tgggagtgga cggagatggc gacggcttca
      181 tcgacctcga tcgcttttcg cctgaagctg cggaggctcg cgatgagact gtcatcttta
      241 gagtgggagc cgtctatgtt tctacgaaga aagtagacct ggaaaagaac gagattatca
      301 tgcgcgagca tggaccatct gactacaagc gcgtggagct ctcggtggga tctgaacttc
      361 ctgattttca gtttacggat ttcgcgggga agaagcgcca gctctctgaa ttccgtggca
      421 aatttgtgct gctcgatttc tgggctttgt ggtgcggtcc atgccggcgt gaattgccat
      481 atcagaaatt ggcgtattcg cgattccagt cgcgcggatt cgagatcctg ggcctgaaca
      541 acgatccgga ttacacgcag gttaagccaa cgctcaagcg caatggtcta gcatggccgc
      601 aggccacaat ggaaagcata gagcctattt taaaacgtta tcgcatacac tacttcccta
      661 ctactctgct cattgggcca gatggaaaga taatttcgct ggaccaaaca cgaaggaagc
      721 agcccggatt gagaggtcag gaattgatcc gatcgctcga cgaactgctc ccaccataat
      781 tctgtcagag accacgaatt cctaaccaat cggtgatttt ggcaaatagg cgtcacgatc
      841 gaatggagga tcttcgtggt ccggaatgac gtcgtcgggc caatcctggg cccacctttg
      901 tcgctgttgc tcgtcagcgt aatacttcag atagatctga ttatcctcgt cgctaccgct
      961 acccgtcaga tcaatcatgt aagcgtagct ggcgttctct ggaaatagaa cggcttcttc
     1021 ccgtaaaccg tcggtataaa gataatggta cagttcgcga tcgcttaggt ggtccgtgtg
     1081 caggagataa gcacctagcg ctgccatcct ttcgatgatc tctttcagtt tgctcttaac
     1141 gttggcatcg tccaggtctt ccaacaccgg aatatcgagg ccggcgtttt ccagcagacg
     1201 gaacaagctg attggctcag cggtttcgta ttccaaaacc tgtcgcaaaa actcctcttc
     1261 gagatctgca ggcattgatt ggagagagtg agtacttcct ccaagcttct ccagttcggc
     1321 tcggagtcgt gcaatgcgct tttcctgatc tggctcgtcg gtcatttgtg tgagtgtcat
     1381 ttgtcagctc cttaagaaat gagaggttta aggtttcgag gccgtgtcga gtttgtgagt
     1441 ggccccggtt tacacttctc gccacggaca tgactcttta atgtttggag ttatgtgagg
     1501 tcgacgaaaa gttgagtgca ttctctttcg tgcagctaga aaagtaaaga gatcatccac
     1561 agattacgcc gattacacga tttcttcgga gacaggcacg gcgcaaaggc gttctagtaa
     1621 agaatccgtg taatcggcgt aatctgtgga ttgtattctt gagttttcac acggtctcta
     1681 agccccggtg ttatttttga actcaagtct tctttgttgc caatggagga gcggtaaaca
     1741 tatcgaggtt cgataaccca aggttctgct cgatttcgga aattcgcttt tggacggctt
     1801 cgaatccatc accaccaaat gtttcagtct cgatctcttc aaacttcgct cccaactcat
     1861 cgagccgccg gtcgcccacc gccgccttcc acgccgggaa gatgatggtg tcttcaatgg
     1921 ccgtgtgatg ctcgtacata cggaccatcg aatccattgc cttgataaat tcctctacac
     1981 ttgcagccgg gatcttatcg cccttcgtca cggacaaaat gtagtcagta atttcacgac
     2041 cgcgtacgtg ttgcgcgatc agtacgtcaa cataagaact cgccgcaccc tgcgcttgtc
     2101 gcaaagacgg caagatgtag gcctcttcga gtttcttctc gtgatagtct tcaccaaata
     2161 ctcgaaagag ctgagccgct ttttccagtt catccgtggg cacgctgcct gcatcctgct
     2221 tcaattttgc cgcgctttct gtgtagacca gcaaaatacg acgaagaata ccgtgttctc
     2281 gcatcaagtc ttcggtggca gtgacttcaa tgccaggggc ttcgtccgac tcttcgcctt
     2341 ctttgcgagg ttcgtcgttt ctcgctccag tggacgagtt ttgcggcatc gcgcaacctg
     2401 ctaagaacac cccggcacca atagtcgaaa ctccaaataa tagctgtctt ctttttagat
     2461 tcaattcttc agactccttt cggtaaccct tgtgactttt gatattaacc tgccgatgac
     2521 gttagactaa gcgtacatca aaaaagacaa gtaaaatcta cgatcatgga taacgccgaa
     2581 atcgcgcgcc ggttctatcg tcttgctgct cttatggaaa ttcgaggcga tgatccgttt
     2641 cggcttcgat cttatcgaaa tgcagcggaa gccatcgagg tctggcccac tccgttacag
     2701 gagatcgctg aagcagaggg cgaaaccggc ctgcaaacca ttccaggcat aggtaaggct
     2761 atcgcgggaa aagtactcga actgctatca acgggaagtt ttgcggcgtg ggaccggtta
     2821 actgcggaaa cgcctgagag tgttttagat ctcaccgaga taccgggcat tggaccaaaa
     2881 accgccgctt tacttcatga gcgctttaaa gtctcatctc gcgctgatct tgtgaagttc
     2941 gtcgccggcg gtggattgga aatggttgat ggaatagggc ccaagaccgc cgaaagaatt
     3001 aaagaagcct tgtcggaatg acattgctac tttaccaatc agtcgctggc atgattgaat
     3061 ttaccgcacg cttttcttca tcacaacttt ttccaaaaac gaggtcgcta tgagcaaatc
     3121 gatcaaacga cgggatttca tgcgcaagag cgccgcagct ggaattgtcg tcgcggcagc
     3181 caaagacaat gttctgggtt ttcccatgat catgaagcaa agcgcaatta aaccggttgt
     3241 catctcgtcc gcaaacggga ataaattcaa aaaccgcggg gacgtaacgg cggtccaaaa
     3301 agctttcacg atgatcacaa atggatctga cgttcttgac gccgtgatcg ccggtgtaaa
     3361 cattgtcgaa ctagacccac ttgatgacag cgttggatat ggcggtcttc cgaatgcaga
     3421 tggcatagtt cagctcgatt catcctgcat gcatggacca aaaaagcgcg ctggagcggt
     3481 tggtgctctt gaaggcgtaa agactccatc tctggtcgcg aaagcagtgc tcgaaaatac
     3541 agaccaccat ctgatcgtcg gcaaaggcgc acaggaattt gcgcgcaaca tgggtttcaa
     3601 aatcgaggac gatttgaaca ctgaccattc gagaaagcag tggctcgaat ggaaacgacg
     3661 cactgatcca tca
//
LOCUS       DASSFP010000297         6778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1708828, whole genome shotgun sequence.
ACCESSION   DASSFP010000297 DASSFP010000000
VERSION     DASSFP010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708828"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFH91_06610"
     CDS             complement(<1..284)
                     /locus_tag="VFH91_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862698.1"
                     /translation="MPTLASRITEPRFPATAIGLEKGMASIVHLERAGGKSCTIRRAA
                     TFNLADAIIRPSFDEPNIGHPDQLAEVLKELATSAGLLRQKRWSLSLPANT"
     gene            complement(308..730)
                     /locus_tag="VFH91_06615"
     CDS             complement(308..730)
                     /locus_tag="VFH91_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6862699.1"
                     /translation="MIGNKFFGKIRQAQPGTKGFTLLELMIVIAIIIILASITLPQYR
                     KTIIQTQEAVLRDDLFKMRSLIDQFAADKGRLPQSLEELASEGYMREVPVDPMTGQKD
                     WTITTGDDPNSTEGQTGMVDVHSASSDPSSAGTPYSEW"
     gene            complement(720..1436)
                     /locus_tag="VFH91_06620"
     CDS             complement(720..1436)
                     /locus_tag="VFH91_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET6862700.1"
                     /translation="MLVKATVGMKRGDLLNRIKSAGRRVPVSERSNRGFSLIELVITV
                     TVMTILTLGVMPLVKVAVRRQKEQQLRETLRQIRFAIDEFHRDTAGLNCTNAGLAGQP
                     GVQPQIGPIDPRSRVMISDCTIFGVDNPDRYPPTLQTLIEGVNVVPRGVGTGGGRGLQ
                     STGTRPSDTSSTLDSQLATKTKVYLRALPIDPMTGKADWDIRSCYDASDSTSWGGENV
                     FDVRSKSRETALNGEKYSDW"
     gene            complement(1440..4160)
                     /locus_tag="VFH91_06625"
     CDS             complement(1440..4160)
                     /locus_tag="VFH91_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET6862701.1"
                     /translation="MRSRSAISLILAISLLSLPITAFGKRGDKNFKRGMQYESAQQWD
                     KAAQEFTLAVAADPSNMEYQLHFRRASFNASQSYMQQGRSLAEQGDFVGAYNAFRQAY
                     GYDPVNELAVSEMERVLRLQAVKEGRTRPPSSTDGQDGTFTPASAQGQAQDVGTPREQ
                     QVTVINYTGDLKTFIRSLAERLNLNVIFDRQSFAQSRSIDINLRDVTTAEALDYIFLQ
                     EGLFFQKLSRRTILVADQARRPQYQQLVLRTFFLSNMKPSDARTLVQQAIPPSVGRPQ
                     TIVVPDDATNSLTVRDTAENVRLIGDLLQSIDKDRAEVVMDVNIYEITRTDFLQWGNQ
                     VGSGNFTIGGSPGFSLLTDNGIVNQSFGRGVNLAPFVGGVPTALAAALVIPPSALTAF
                     ANKQNTKLIASTQVHAFNGEESTARIGQRVPVQTAQAYPFGIQTGTPSPNTGNVFPSG
                     GFPVINYEPTGLTLKFTPQVFPNLDVQVKMNIESKDVFGATTLTPTFTERTITGTARI
                     QNNRTMMLASVSSEAESVGRQGLPILGGLPILGRFFTAPTRDNKQIDIVISVTPRVLR
                     APTVTPRDEEMRPSGTLSSPTTGSLAQMVKEADRDDQIAAARSIPRDVAMQLPDRPVE
                     VATPKQPQPSGAVAQNTTAPSTSVSTQAAPAPPTNTQPIAPVNTPTSGTVAQSNPPAI
                     LNTPVVQQSTQPAGTAQPIAKDKAAVPDEIPSFVPAPKSLISDRAAGEVATVNSSPAG
                     TQNAVLSSLPKPVGASLNTGSTDKRVAQLKFLPAQDVMKVGEKRRYAIQLESDVGLTV
                     ALLALKFDPRVVKVNGISAGSLIVLAGDDAPTFTQFKSNQSGICMVSISTPEGKSAIR
                     GTGSLILIDVEAIGLGDASFSFDKDTLHLVATDARDVSSAITQGVATVQQ"
     gene            complement(4356..6059)
                     /locus_tag="VFH91_06630"
     CDS             complement(4356..6059)
                     /locus_tag="VFH91_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET6862702.1"
                     /translation="MSQSTENDSPILQNTVPLPGTGSMVADDHQPPEMKAARELARRY
                     RLPFVDLLPPDKESPIDYNLFTEVPVDLMVRHQFVPLRRDAKGLHIAMADPTDLERLD
                     ELAHTLKTRVVPYVATAGAVEVVLRKGDTTQRVLQEAASGFRISLVRETEAGEEVLDL
                     DRLATDSEMSPIIKLVDTIIYNAMESRASDIHIETRDTEVQVKYRIDGALYAKVDPID
                     LAYHQTLISRIKVMSELDIAERRVPQDGRFRVRYKGRNVDFRVSIMPTIHGEDAVIRI
                     LDKEQINEEFKNLNLNVVGFAEEDLRKFRRYIAEPYGMVLVTGPTGSGKTTTLYAALN
                     EIRNEEDKIVTIEDPVEYQLHGITQIPVNEKKGLTFDRGLRSILRHDPDKIMVGEIRD
                     TETAQIAIQSALTGHLVFTTVHANNVIDVIGRFLNMGVEPYNFVSSLNCVLAQRLIRM
                     LCPYCKRLYHPGDTELTESGLRPEIYRTHPFYLNVGCDACNHTGYRGRTAIHELLDLS
                     DNIREMIVERRPGSEVRRAAEAEGLTSLRESALQKVFSGVSTLHEINRVTFVEELSSI
                     K"
     gene            complement(6077..>6778)
                     /locus_tag="VFH91_06635"
     CDS             complement(6077..>6778)
                     /locus_tag="VFH91_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6862703.1"
                     /translation="ASACMVMFLSIYVVPRMSELFSGFGNELPTVTQIVVGLSRWLSA
                     NVIWFGPLLIGAAILLFIWSRTPMGKLQLSALILKIPLAGSLAVQLSVAQATRSLATL
                     LAGGITLVESWEIASESITNLELRRRSSSILPMIREGRSFTESLETAGWLPPLAIDMI
                     GIGERSGSLREMLDEVAVFYDAESEVRLEQLTTTLEPAILVVMGGVVVTILLAIYLPI
                     IQSISNGAGVTGHRQ"
BASE COUNT         1582 a         1752 c         1732 g         1712 t
ORIGIN      
        1 gtattcgcag gcaaactcaa cgaccaacgc ttttgacgaa gcaatcccgc gctggtggcc
       61 aattctttta agacttccgc aagttgatcg ggatgaccaa tatttggttc atcaaagctc
      121 ggacgaatta ttgcgtcagc aagattgaaa gttgctgcgc gtcttattgt gcatgatttt
      181 ccacccgctc gttcgagatg cacgattgac gccatcccct tttcgaggcc tatagcagta
      241 gcgggaaatc gcggttcagt tattcgtgat gcgagtgtgg gcattcttta aaattgctgg
      301 tgacgtttca ccattcactg tatggagtac ctgcggacga agggtccgaa gaagcgctgt
      361 gtacatcaac catccctgtt tgaccttcgg tcgagttggg atcgtctccg gttgtaatgg
      421 tccaatcctt ttgacctgtc atcgggtcaa ccggaacctc tcgcatataa ccttcgctgg
      481 ccagttcttc gagcgactgt ggcaatcgtc ctttatcggc agcaaactgg tcgatcaacg
      541 agcgcatttt aaatagatca tctcgaagga cggcctcttg agtttgaata atggtctttc
      601 gatattgtgg cagagttatc gaggccagga tgataatgat cgcaattacg atcattagct
      661 ccagcaaggt aaaacctttc gtccctggtt gtgcctgtcg gatttttccg aaaaacttat
      721 taccaatcac tgtacttctc tccgttaagt gcggtctcgc gtgacttgga ccgcacatca
      781 aaaacattct cgccacccca tgaggttgaa tctgaagcgt catagcaaga gcgaatatcc
      841 caatcagctt tcccggtcat gggatcgatg ggaagagctc ggagatacac tttagttttc
      901 gtcgcaagct gcgaatcgag cgttgagctg gtatcagatg gacgagtgcc agtgctctgt
      961 aatcctctgc ctccgcccgt accgacgcct ctcggaacaa cgtttacacc ttcaatgagt
     1021 gtttggagtg ttggcggata acgatcagga ttatcaaccc cgaaaattgt gcaatcacta
     1081 atcataacgc gacttcgcgg atcgattggt ccaatctggg gctggacgcc aggctggccc
     1141 gcaaggcccg cgttcgtaca gttaagccct gccgtgtcgc gatgaaactc gtctatcgca
     1201 aacctgattt ggcgtaatgt ctctcggagt tgctgttctt tttgacgtcg aacggcaacc
     1261 tttaccagag gcatcacgcc tagagtcaag atggtcatga ccgtaacggt gatgacgagc
     1321 tcgatcaacg agaagccccg gttgctccgt tccgacacag gaacgcggcg acctgcagac
     1381 ttaattcggt tcaacaggtc accgcgtttc attccaactg tagctttcac taacatctac
     1441 tactgctgaa cagttgcgac accctgagtt attgccgacg atacatctcg agcgtcggtt
     1501 gcaaccagat gcaatgtgtc tttgtcgaag ctaaatgaag catcacctag accaatcgcc
     1561 tcgacatcga tcaagatcaa cgatcccgtt ccccggatag ctgatttgcc ttccggagtt
     1621 gagatcgaca ccatacaaat accagactga tttgacttga attgtgtaaa ggtcggggcg
     1681 tcatctccag ccaggacaat caagctcccg gcacttatgc cattaacctt cacaactctt
     1741 ggatcaaatt ttaaagcaag caaagccacg gttaagccaa cgtctgactc aagctggatt
     1801 gcgtaacggc gtttttctcc aaccttcatc acgtcctggg ctggcaggaa cttgagctga
     1861 gcaactcgct tatcggtcga gccagtgttc agagacgcgc caaccggctt tggcaaactg
     1921 gacaacactg cattctgagt tcctgctgga ctcgaattga cagttgcaac ctccccagcc
     1981 gcccggtcac tgatgagtga ttttggcgcc ggcacaaacg agggaatctc atcaggcact
     2041 gccgctttgt ctttagcgat cggctgtgcc gttcctgcag gttgagtact ttgctgtacg
     2101 actggtgtgt tcaatatggc tggaggattc gactgagcca cagttcccga cgttggtgta
     2161 ttgacgggtg caatcggctg cgtattcgtg ggtggcgctg gagctgcctg cgtactcacg
     2221 gaagtactcg gagcggtggt gttttgggca acagctcctg acggctgcgg ttgttttgga
     2281 gtcgcaacct caactggccg atccggcagc tgcatagcga catcacgcgg gatactacga
     2341 gcagccgcta tctgatcgtc tcggtcagct tccttcacca tctgggcgag cgaaccggta
     2401 gttggtgacg agagcgttcc acttggtctc atctcttcat cgcgcggagt tactgttggt
     2461 gctcgtagta cgcgcggagt tacagagatg acgatgtcaa tctgcttatt gtcacgcgtt
     2521 ggagccgtga aaaatctgcc caggattggc aagccgccaa gaatcggcaa gccctgacga
     2581 ccaaccgatt ctgcctcgga cgagacactt gccagcatca tggtccgatt attctgtatg
     2641 cgagcagttc cagtaatagt gcgctcagtg aatgttggcg tcagagtggt cgcgccgaag
     2701 acatccttcg actcgatgtt catcttcacc tgaacgtcca gattcggaaa cacctgcgga
     2761 gtaaatttga gagtcagtcc ggtcggctcg taattaatta caggaaagcc accactcggg
     2821 aaaacgttac ctgtatttgg actaggtgtt ccagtttgaa taccaaaagg gtatgcctga
     2881 gcggtctgta ctggaactcg ttgaccgatt cgggcagtcg actcttcgcc gttgaatgcg
     2941 tggacctgcg ttgaagcaat cagtttcgta ttctgtttat tcgcgaaagc cgtcaaagcc
     3001 gatggtggga ttacgagagc agccgccaat gcggttggaa ctccgccaac gaagggagct
     3061 agatttacgc ctcggccaaa agattgattg acaataccgt tatcggttag gagcgagaaa
     3121 ccaggtgaac caccgattgt aaagttgcca cttccaacct gattacccca ctgcaagaag
     3181 tccgttctgg tgatttcgta aatgttgaca tccatcacca cctcggcccg gtccttgtcg
     3241 atgctctgca gcaagtctcc gatcaggcgg acgttttcag cggtatcgcg aacagttaag
     3301 ctgttagtcg catcgtccgg taccacaatc gtctggggcc ttccaactga cggtggaatc
     3361 gcctgttgca ccagcgttct tgcatcgctg ggcttcatat tggaaaggaa aaatgtgcgt
     3421 aacactaatt gctggtattg aggccgacga gcctggtcag caacaaggat ggtccggcga
     3481 cttaacttct ggaaaaacag tccttcctgg agaaagatgt aatcgagcgc ctctgcagtt
     3541 gtgacgtcac gtagattgat atcgattgaa cggctctggg cgaaagactg tcgatcaaaa
     3601 atcacgttta gatttaatcg ttctgccaga ctgcgaataa aggtctttag atcaccggtg
     3661 taattaatca cagtgacctg ttgttcgcgt ggagtcccaa catcttgagc ctggccttgt
     3721 gcactcgctg gggtgaaggt gccgtcctga ccgtcggtag agctgggagg tcgcgttcta
     3781 ccctctttga ctgcctgcaa ccgaagcacg cgctccattt ctgagacggc aagttcgttc
     3841 acaggatcat acccatacgc ctgacggaag gcgttgtaag cacccacgaa gtcgccctgt
     3901 tcagccagtg atcggccttg ctgcatgtag ctttgtgaag cgttgaagct tgcccggcgg
     3961 aagtgcaact gatactccat gtttgaggga tctgccgcga ctgcgagggt gaactcctgg
     4021 gccgctttgt cccactgctg ggcactctca tactgcatgc cgcgtttgaa attcttatca
     4081 cctctttttc cgaacgcagt gatcggcagt gacagcaaac tgatggcaag gattaaagaa
     4141 attgcggatc tacttctcat tttgattctc atggcttttc tgctcgggag gtggacagtt
     4201 tggcgacggt aataatgccg cgccgaataa gatgcaagat ccgcgctggc aaatccttac
     4261 acgcggctcg caacttcaag ttccgtttaa agaccaatct actcgggttg attttacttc
     4321 gttcaaggtg ccggcttgtg tggcggttcc ggcacctatt taatggaact cagttcttca
     4381 acgaaggtca cccggttaat ctcgtgtaaa gttgaaaccc cgctgaaaac cttctgtaat
     4441 gctgattccc taaggctggt caatccctcg gcttctgctg ctcgacgtac ttccgaccct
     4501 gggcgtcgct cgacaatcat ttcgcgaata ttgtcggata agtcgagcaa ttcgtgaatg
     4561 gctgtacgac ctcgataccc ggtgtggtta cacgcgtcgc acccgacatt caaataaaaa
     4621 ggatgagtcc gatatatctc tggcctgagg ccgctttcag ttaattcggt gtcaccggga
     4681 tgatacaacc gtttgcagta tgggcaaagc atccggatga gtctttgagc caaaacgcaa
     4741 tttagggaag aaacgaagtt gtatggctcg acacccatgt tcaggaatcg gccgatcacg
     4801 tcgatgacat tattggcgtg aacagtggtg aaaacgaggt gtccagtcaa cgccgactgg
     4861 atagcgatct gggctgtttc agtgtcacgg atctccccga ccatgatctt gtcgggatcg
     4921 tggcgcagta tcgaacgcaa accacggtca aacgtcaggc ccttcttttc attcaccggg
     4981 atttgagtga tgccatgaag ttgatattcg accggatcct cgatcgtgac gatcttatct
     5041 tcttcatttc tgatctcatt gagcgcggca taaagggtag tcgtctttcc tgatccggtt
     5101 gggccggtta ccaggaccat gccatatggt tcagcaatat agcgtctgaa ctttctcaag
     5161 tcctcttcgg caaaaccaac gacattaagg tttaggtttt tgaactcttc gttgatctgc
     5221 tctttgtcga gaattcggat aaccgcgtcc tcgccatgaa ttgtcggcat gatcgaaacg
     5281 cggaagtcga cgttgcgacc tttgtaacga actcgaaaac ggccgtcctg aggaactctt
     5341 cgttccgcaa tatccagctc cgacatgact ttgatacgtg atatcagtgt ctggtgataa
     5401 gcaagatcga tcggatcgac tttcgcatag agcgctccgt cgattcggta cttgacttgt
     5461 acttccgtat cacgagtttc gatgtgaata tcggatgcac gcgactccat cgcgttgtag
     5521 ataatcgtgt cgaccagttt gatgatcggc gacatctcag agtctgtcgc taatcgatcg
     5581 aggtctagta cttcttcgcc ggcctcggtc tctcgaacaa gtgagatacg gaaacccgat
     5641 gcagcctctt gcagcactcg ctgagtagta tcaccttttc gcaggaccac ttcgactgct
     5701 ccggcagtgg ccacatacgg aacaaccctc gttttcaagg tgtgggccaa ctcgtccagc
     5761 cgctcgagat ccgttggatc agccatagcg atgtgcagtc cctttgcatc acgtcgcaac
     5821 ggcacgaatt gatggcgaac catcaagtca accggcacct cggtgaaaag gttgtaatcg
     5881 atgggtgatt ctttgtctgg tggtaataag tcgacgaacg gcaaccgata acggcgcgcg
     5941 agttcgcgcg cggccttcat ttccggcggc tgatggtcgt cggcaaccat tgaaccggtg
     6001 cccgggagag gaacggtatt ctgcaaaatc ggactgtcgt tttctgtgga ttgactcatg
     6061 aacttactag taaaacctat tgtcgatgtc cggtgacccc ggcgccgttg gaaatcgact
     6121 gtatgattgg gagatagatg gccagtagaa tcgtcactac gacgccaccc atcacgacca
     6181 gaatcgcagg ctccagcgtg gtcgtcaact gctcaagcct gacctcagac tcagcgtcat
     6241 aaaagacagc tacttcgtcc aacatttctc gcagacttcc agaccgttca ccgataccga
     6301 tcatgtcaat tgccaacggt ggcaaccatc cagctgtttc caaactttca gtaaatgacc
     6361 tgccttctcg aatcatgggc agtattgatg aacttcggcg ccgcaactcg agatttgtta
     6421 ttgattcgga cgctatttcc caggactcga ccagagtgat accgcctgcc aaaagagttg
     6481 caagtgacct tgtcgcctgc gccacggaca gttggaccgc caaactgccc gccagaggaa
     6541 ttttcaagat cagggcgctt agttgcagtt ttcccatcgg tgttcgcgac cagataaaga
     6601 ggagaatcgc tgcgcctatt agcaatggtc caaaccagat aacattcgct gacaaccaac
     6661 gggaaagacc aacaacgatt tgagtgactg tcgggagttc attaccaaag ccggaaaaca
     6721 gttccgacat ccttggcacg acgtaaattg agaggaacat aaccatacat gccgatgc
//
LOCUS       DASSFP010000298         5156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_81971, whole genome shotgun sequence.
ACCESSION   DASSFP010000298 DASSFP010000000
VERSION     DASSFP010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81971"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..847
                     /locus_tag="VFH91_06640"
     CDS             101..847
                     /locus_tag="VFH91_06640"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HET6862704.1"
                     /translation="MSDAIQAKRRSFRPQVKKQVTSFVSSRRSKMFGLAEIAGLSLSF
                     LVLLLVIFSYFYFLVPARTHLEELRSDRTQRQTNVDTLTSVVNTDQNKKENITRIITS
                     FDKFESAGLQQEEPGRIGLYEELNRLIIKNGLRNTSGPAYTPLEPVGAKVAGGRTVST
                     RWQSVYPGVAVSVTLEGSYQSLRHFIQDIERSRLFVIINEIELQRATTNNGPVTASET
                     VGNKASLVSLQLNMATYFQRALDGSMDKSR"
     gene            854..1762
                     /locus_tag="VFH91_06645"
     CDS             854..1762
                     /locus_tag="VFH91_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862705.1"
                     /translation="MEFVDLSKPGEKKKLLWAAILGLVAVLFLWWTLFGFGSSKKKPS
                     TVAGPTSSPTPSTGQTQAPNSPSGAVADIAQFAEIVYPGSTPSVGEPNRNIFAYYEPP
                     PKQDPVTTPKPATPTPTPPVLLAAVNPSNVYAKTPDFTLEASGDKFTPDLHIFVDNRE
                     LPTKYIGPQQLSTTVPAAFIANPGNREVVVRNQDGRVYSLPVTMNVAAPPTPNFTYVG
                     IVSTVPRIDVAYLQDRSSKDILNVQRGDVVGGRFRVTSISVKEIVLVDTTLKIKHSLA
                     MVESDKNPTSPLNRPTPKVDAEDDEP"
     gene            1749..2477
                     /locus_tag="VFH91_06650"
     CDS             1749..2477
                     /locus_tag="VFH91_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862706.1"
                     /translation="MTNLSEKTNHNILGSSLSGVTGALRGRSGATRSSEGGYALVALL
                     ALMTIVALFAMAAAPRIQQQVQRDQEQEAIFRGEQVADAILAYYAFRNQQYRVNGDQA
                     LPTSIDQLLEGVPIPGGSKTRQILRPSATLDPLSNTHQWRLVHPRSEELIDFQQAVMF
                     YAGNALPPAQGYRAQIQQFAVPQLTALVNTGTGPREPSGGSAGEDSSGPFVGVASRSK
                     RDSVLHFYGIERHELWIFTPLFRN"
     gene            3085..3543
                     /locus_tag="VFH91_06655"
     CDS             3085..3543
                     /locus_tag="VFH91_06655"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET6862707.1"
                     /translation="MIRVTLVEPEIPPNTGNIARLCAALNVPLHIVGVTGFRMDDRAV
                     RRAGLDYWPEVQLHRHHTLADLNEALPEARLVFLTTKTDRSYLDWRFESRDCLVFGRE
                     TRGLPEQLLKDNWENCLTIPMTNPRVRSLNLATAVAIVLAEALRQTTQTQ"
     gene            3622..>5156
                     /gene="typA"
                     /locus_tag="VFH91_06660"
     CDS             3622..>5156
                     /gene="typA"
                     /locus_tag="VFH91_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET6862708.1"
                     /translation="MKKRSDIRNIAIIAHVDHGKTTLVDAMLRQSGTFRENEQVRERV
                     MDSMDLERERGITIMAKNTAVRFRNIKINIVDTPGHADFGGEVERVLKMVDGVMLLVD
                     AAEGCLPQTRFVLRKALEARLPAIAVVNKIDRSDARPAEVVDEIYELFLDLDATDEQI
                     EFPILYAISREGMAKLKLEDDSDSLRPLFEQIVETIPEPKELRTDSLQLLVANLDYND
                     YVGRLAIGRIFSGEIAVGDQVAVMKPDRSIQKTRVSQLYVFEGLKREAVERAGFGEIV
                     ALAGIENINIGDTITSIESPQQLPVIAVDEPTIAMIFSVNNSPFAGREGKFVTSRQVK
                     ERLDKEILGNVAIRVDETESPDQFKVSGRGELQLAILIEMMRREGYELQVSKPEAITR
                     EINGQTFEPVEAVVVDCPDEFIGVITEALGRRKGQMTRMVNHGTGRVRLEFETPSRGL
                     IGFRNEFLTETKGTGLLNTMFLRWGPWQGPMRGRATGSLVADRTGDTTTYALFNLQER
                     GTL"
BASE COUNT         1341 a         1290 c         1366 g         1159 t
ORIGIN      
        1 gcaccgaata tgaactcggt gttgtttatc agccgcgtcc aggttttgcg atcgaaagag
       61 ttgcggccgt agaccgggcg gccgataagg aggaagcaaa atgagcgacg ccattcaagc
      121 aaagcgccga agcttccgcc cgcaagttaa gaaacaagtg accagcttcg tctcttctcg
      181 acgcagtaaa atgttcggtc tcgcagagat cgccggtctg tcgttgagtt ttctcgtcct
      241 acttctggtc atcttcagct atttctattt tctcgtccca gcccgtactc accttgaaga
      301 acttagatct gatcgtacgc agcgacaaac caacgtcgac acattgacca gcgtagtcaa
      361 cactgatcag aacaagaagg agaacataac tcggataatc actagcttcg acaagtttga
      421 gtctgcaggc cttcagcagg aagagccggg acggatcgga ctatatgaag aactcaatcg
      481 gttgattatc aagaacggcc taagaaatac ttctggtcct gcttatacgc ctcttgagcc
      541 ggtcggcgct aaggtggccg gaggaagaac cgtcagtact cgatggcaaa gcgtatatcc
      601 tggagtggcc gtgtcggtta cgctggaagg ctcatatcaa agcctgagac acttcataca
      661 ggatattgaa agaagcaggc tcttcgtcat aatcaatgag atcgagttgc agcgagccac
      721 cacaaacaac ggacccgtaa ccgcttctga aacggttggt aataaggcgt ccctggtgag
      781 cttgcagctg aacatggcaa cttatttcca aagagccctc gacgggtcaa tggacaagag
      841 caggtaaggc cacatggaat ttgttgactt aagtaagccg ggcgaaaaga agaagctcct
      901 gtgggccgcc atcctgggtc tggtagccgt cctgtttctg tggtggacat tgtttggatt
      961 tggtagttcg aagaagaaac cttcgactgt tgccggaccc acctcttcgc caaccccgtc
     1021 tacgggacaa acgcaagcgc ccaactcgcc ctctggtgcg gttgcagata ttgcgcagtt
     1081 tgcggagatt gtttatccgg gttcgactcc aagcgttggc gagcccaatc gaaatatatt
     1141 tgcctactac gaacctcccc cgaaacaaga cccagtaacc acccctaaac cagcgacacc
     1201 gacaccaacg ccaccggttc tgttggcagc ggtcaatccg tctaacgttt acgccaagac
     1261 cccggacttc actctcgaag cttcgggcga taagttcacc ccggaccttc atatctttgt
     1321 ggacaatcgg gaactgccca cgaagtacat tggtccccaa cagctctcga caacagtccc
     1381 tgctgctttc atagcaaatc ctggtaaccg tgaggtggtg gtgcgaaacc aggacgggcg
     1441 cgtctattca ctcccggtta caatgaatgt agctgcgcca cccacgccca acttcacata
     1501 cgtaggtatt gtcagtaccg tcccgcgtat cgatgtggct tacctgcagg atcgctcgag
     1561 caaggacatt ttgaatgtac agcgtggtga tgtcgtcggc ggcagattcc gtgtgaccag
     1621 catctcagta aaggagatcg tcctcgtcga tacgactctc aagatcaaac acagcctggc
     1681 gatggtcgag agtgacaaga atccgacgtc acctttaaac cgtcccactc cgaaagttga
     1741 cgctgaagat gacgaacctt agcgagaaga ccaaccacaa tatattaggg tcgtccctct
     1801 ccggcgtgac cggagcgttg aggggcagat cgggcgcgac gaggtcttcc gagggtggtt
     1861 atgcgctggt cgctctcctg gcgctgatga ccatcgtggc actctttgcc atggcagcgg
     1921 cgcccagaat tcagcaacag gtgcaacgcg atcaggagca ggaagcgatc ttcagaggtg
     1981 aacaagtcgc cgatgcaata cttgcttact acgcgtttcg caaccagcag tatcgagtga
     2041 atggcgatca agcgctaccg acatcaatcg atcaattgct tgaaggcgtc ccgattccag
     2101 gaggatcaaa gacgagacaa attcttagac caagcgcaac gctcgatcct ttgagcaaca
     2161 ctcatcagtg gcgtttggtc catccgcggt ccgaagaact gatcgatttt caacaggctg
     2221 tcatgtttta tgccggcaat gccttgcccc ctgcgcaggg ctatcgtgcg cagattcaac
     2281 agtttgcggt tcctcagctc accgcgctgg tcaataccgg aactggacca agagaacctt
     2341 caggtggcag cgcgggtgaa gactcgagtg gtccattcgt cggagttgcg agtcgcagta
     2401 agcgagattc ggtcttgcac ttttatggaa tcgaacgaca cgagctttgg atttttactc
     2461 ctctcttccg caattgatct tggaccaccg tcgatctcct tctcaactcg gctcgaacat
     2521 ctcaatactc taagccgaaa ttcgtaacgc ctcgccgtct ctttttccct ggcccaaccc
     2581 tgcgcgatcg agatcgcttc aagcgatctt tagcggctga tgacgagcaa ctcaggtgag
     2641 cggagcgaaa caaaaggcga aaaaatattt gtgcgtcaca gtaataagtt attgacttct
     2701 gttgcattta cgtttatata gcaagatgtt gtgcgccgtt tactctagcc cacaagaaca
     2761 aagcaagcac actatcttgt ggtcatgaag aaaacaaaaa aaactaatct cgaaagaagg
     2821 agcacataga agctatggca actaaaaaag gtggaaagaa ggccgcgaag aagagcggcg
     2881 gaacaaagaa agccgcaaag aagggcggag ccaagaaagc cgcaaagaag agataactcg
     2941 aaccagcagg ttacattacg aaccttccac tggcgcacac aaacttctga ggcccgcggg
     3001 gccttacaaa agaaacgacg agaccttcaa gcgaagctcg tcgtttcttt ttgttttcgt
     3061 taatctgatc gagttgaggt ccgaatgatc agagtgactc tagtcgaacc ggagattcca
     3121 cctaacacag gtaacatcgc ccgcttgtgc gcagcgctca atgtcccgtt acacatcgtg
     3181 ggagtaaccg ggtttcgcat ggacgaccga gctgttcgcc gcgcaggact cgactactgg
     3241 ccggaagtcc agttgcaccg gcaccacact ttggctgatc tcaatgaagc tttaccggag
     3301 gctcgactgg tgtttttaac gaccaaaact gatcgcagct accttgattg gagatttgag
     3361 agccgcgatt gcctcgtatt cggcagagag actcgaggtc ttcctgaaca attactcaaa
     3421 gataactggg agaattgcct cacgatcccg atgactaatc ctcgcgttcg gagtcttaat
     3481 ctcgcaaccg cagttgctat tgtccttgcc gaagcactcc gccagactac acagacacaa
     3541 tgagagagcc tggcgcaaag gcggagaatg cgcggcgatg gtaagatgcg tcgtgttttc
     3601 ggaaggaatg tgaagtaatt gatgaaaaag agatctgata tcagaaatat cgcgattatc
     3661 gcgcacgttg accacggaaa gacaacactg gttgatgcaa tgcttcggca gtcagggacc
     3721 tttcgtgaaa atgagcaggt tcgtgagcgc gtgatggact caatggacct ggaacgtgaa
     3781 cgcggcatta cgatcatggc aaagaatacg gctgttcggt tccgcaatat aaaaatcaac
     3841 attgtagaca cgccgggtca cgcggacttc ggaggtgaag tcgagcgcgt gctaaagatg
     3901 gtcgatggag tgatgttgct cgttgacgct gctgaaggtt gtctgccgca gaccagattt
     3961 gtattgcgta aagcactcga agcacgttta ccggctattg ctgtcgtcaa taagattgat
     4021 cgtagtgatg cacggcccgc cgaagttgtc gatgaaatct atgaattgtt cctcgatctt
     4081 gatgcgacgg atgaacagat cgagtttcca atcctgtacg ccatctctcg tgagggtatg
     4141 gcaaagctaa aactcgagga cgattccgat agtctcagac ctttgtttga acagattgtc
     4201 gaaacgatcc cggaaccgaa agaacttcgg actgactcgc tacaactgct cgtagctaac
     4261 ctggattaca acgattacgt gggtcggcta gctatcggaa gaatcttttc gggcgagata
     4321 gcggtcggcg accaggttgc ggtgatgaag ccggatcgat cgatccagaa gacgcgagtc
     4381 tctcaacttt acgtttttga aggtctcaag cgagaggcag ttgaaagagc cggatttgga
     4441 gagatagtgg cgctcgccgg aatagaaaac atcaacatcg gcgacaccat tacgtcgatc
     4501 gagagtcctc aacagcttcc ggtgattgcc gtcgatgagc cgacaatcgc catgatcttt
     4561 agtgtcaaca attcaccgtt tgccggacgc gaaggcaaat ttgtcacatc gcgccaggtg
     4621 aaggagaggt tggacaagga gattctcggg aacgttgcta tcagagtcga tgaaaccgaa
     4681 tcaccggatc agtttaaagt ttccggtcga ggcgaacttc agcttgcaat cctgatcgag
     4741 atgatgcgac gggaaggata cgaattacag gtttcgaaac cagaagctat cactcgcgag
     4801 atcaatggac agacatttga gccggttgag gctgtggtgg ttgactgtcc cgacgagttc
     4861 attggagtaa tcactgaagc tttgggaaga cgcaagggtc aaatgaccag gatggtgaac
     4921 catggcaccg gtcgtgtgag gttagagttc gaaacacctt cccgtggatt gatcggtttt
     4981 cgcaacgagt tccttactga aacaaaagga actggattac tgaatacgat gtttttacgt
     5041 tgggggccgt ggcaaggtcc gatgcgagga cgcgctaccg ggtcattggt tgccgaccgg
     5101 acgggtgata cgacaactta cgcgcttttc aacctgcaag agcgaggaac tctgtt
//
LOCUS       DASSFP010000299         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1569897, whole genome shotgun sequence.
ACCESSION   DASSFP010000299 DASSFP010000000
VERSION     DASSFP010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569897"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFH91_06665"
     CDS             complement(<1..782)
                     /locus_tag="VFH91_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862709.1"
                     /translation="MSTLIIHGHFYQPPRENPWTGIIDNQPSAAPYHDWNERIHAECY
                     GPNAFARIGDGDTVQVVNNYANISFNFGPTLLSWLEEKYPRTYYQILKADADSASRRG
                     GHGNAIAQAYGHAILPLCNARDLLTQIIWGQEDFRYRFRREPESLWLPETACNNQTLD
                     VLIDQGLRYVILAPEQAKRVKQNGEWIDVNGGTVDTGRPYRYVHTDGSGRFLAVFFYN
                     GPLARAIAFEKLLTSSQLLIDRFVEVAQYRPLVHVATDGETYG"
     gene            complement(873..1841)
                     /locus_tag="VFH91_06670"
     CDS             complement(873..1841)
                     /locus_tag="VFH91_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6862710.1"
                     /translation="MSKISEPKQQHVLAADLGGTHLRVAIVNSCGQIVTQVKQTTPTE
                     STDPETIVRAIVEAAERCVSNEEYGKPAAASIVVPGAVDVDNAVVVQAPNLPSLTNFD
                     LKSALETRLGLNVVLENDANAAAVGEMWLGAAKGCRDVISVTLGTGVGGGIILNGVLW
                     RGAHGSAGEIGHTTVDPLGGLKCKCGNSGCLELFASATAITRMTNAQRLEFPNTKLPR
                     EDLNAEMVYDAGKAGDELALSVFRQFGMYLGIGLANLINLLDPEIIVIAGGVVNGWTM
                     FEKVMRDQVNERSFRLAAARVRIAPAECGDNAGLLGAARLALDLCQ"
     gene            complement(1872..3716)
                     /locus_tag="VFH91_06675"
     CDS             complement(1872..3716)
                     /locus_tag="VFH91_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET6862711.1"
                     /translation="MKLVSIDWLIIAAYFVLSLSIGLYYAKRAGRSTEEYFLSDRSMP
                     WWLAGTSMVATTFAADTPLAVTEMVARNGVAGNWLWWSMLASGMLTVFFFARLWRRAR
                     VMTDVEFVELRYSGKPAAFLRGFRALYLGFPINLIIMGWVNLGMAKVLSGTLGLAKWQ
                     ALALCLGITFLYSILSGFWGVVVTDAVQFVIAMAGSIALAFFAVAAVGGLDNLKARLA
                     GVTPVGSTEPFGPGGAIALLPDGSAVWMLPLLTLSVYLAVNWWASWYPGAEPGGGGYV
                     AQRIFSAKNEKHGLLATLWFNIAHYALRPWPWIIVALCSLVLYNGTTLNPETGKPDAA
                     FGYVQVMNDYLPIGFRGLLLASFAAAYMSTISTQMNWGASYVINDFYRRFVKPQATEK
                     HYVFASRVATLIIVLLSIVVTYFMNRITSGWELVLALGAGTGLVYILRWYWWRINAWS
                     EIAAMASALIVSLTLLQFSIFDSSTPIGFAQQILTTVAVTTIVWIVVTFATSPEPMSK
                     LISFYQNVEPAGPGWRPVVTAVGLSERQGEIWPNVLNWILGIILVYSILFGIGQMIFG
                     VWSKTILLLALAVGAGVGLLWNLNRTGWAGFSEVAESRAGEALVEGAD"
BASE COUNT         1016 a         1068 c          934 g          880 t
ORIGIN      
        1 ccgtaagttt caccatcagt ggctacatgg accaatggtc tgtactgcgc aacctccacg
       61 aatctatcta tcagtaactg gctggaagtc aacaattttt caaacgcgat cgcccgcgct
      121 aatggtccgt tataaaagaa aaccgccaga aacctgcctg atccatcagt atgaacgtag
      181 cgataaggtc gacctgtatc cactgtgccg ccgttcacgt cgatccattc gccattttgc
      241 tttacgcgtt tcgcctgttc aggggcgagg attacgtagc gaagaccctg gtcgatcagg
      301 acatccagcg tctggttgtt acaggctgtt tcaggcaacc agagggattc aggttctcgc
      361 cgaaagcggt aacggaagtc ttcctgaccc cagatgattt gagtcaacag atcgcgagcg
      421 ttacaaaggg gtaaaatggc atgtccataa gcttgtgcaa ttgcgttgcc gtggccgcct
      481 cgcctgctgg cactatcggc gtctgctttt aaaatctgat agtaggttcg aggatatttc
      541 tcttcgagcc atgagagcag ggttggccca aagttgaagc tgatgtttgc atagttattg
      601 acgacttgaa cggtatctcc gtcgcctatc cgtgcgaaag catttggtcc ataacactcc
      661 gcgtggattc gttcgttcca gtcgtggtac ggagccgcgc ttggttgatt gtcgatgatg
      721 cctgtccagg gattctcacg cggaggctga tagaagtgtc cgtgaataat taaggtgctc
      781 attgccggtt ggaggaagta tacgacactg agcgcctgtt tttagcacat cccatgagca
      841 aagaaacata aagaggaaat tagccggaca gcttactggc acagatccag agcgagcctg
      901 gcagcgccga gcaatccggc attgtcacca cactcagcgg gagctatgcg gacgcgggct
      961 gcggcgagtc gaaatgagcg ctcgttgacc tgatcccgca tcactttttc gaacatcgtc
     1021 caaccgttta ctaccccacc cgcgataacg attatctccg ggtcgagcag attgatgagg
     1081 ttagccaggc cgattccaag atacattccg aattgccgaa atacgcttag cgccaattca
     1141 tctccagctt tgccggcgtc ataaaccatt tcggcgttca gatcttctct cggaagctta
     1201 gtgttgggaa actccagacg ctgcgcattt gtcatccgcg ttatggccgt agcagatgca
     1261 aacaattcaa ggcaacctga attaccgcac ttgcacttga gaccaccaag tggatcgact
     1321 gttgtgtgtc ctatctctcc ggcggatccg tgcgcgcctc gccacaaaac gccattcaag
     1381 atgattccgc cgcccactcc ggttcccaac gtaacgctaa ttacatctcg acaacctttt
     1441 gcggcgccaa gccacatctc tccaaccgct gccgcattcg catcgttctc aaggaccaca
     1501 ttcaatccaa gccgcgtttc gagcgctgat ttcaaatcaa agtttgtgag tgaaggaagg
     1561 tttggagcct gaacgacgac tgcgttatcg acatcaactg caccaggtac gactatcgat
     1621 gctgcagccg gtttgccata ttcctcattg cttacgcacc gttctgcagc ttcgacaatg
     1681 gcgcggacga tcgtttcagg atccgtcgat tctgtcggag ttgtctgttt tacctgggtg
     1741 acaatctggc cacatgaatt aacgattgca acgcgcaggt gagttccacc tagatccgcc
     1801 gccaggacat gttgttgttt aggttccgat attttgctca tcaagagtct ctttacgaac
     1861 tctcactcca attagtctgc accttcaaca agggcctcgc cggcacgaga ctcggcaacc
     1921 tcagagaatc ctgcccatcc ggttcgattg agattccaaa gtagtcctac ccccgctcca
     1981 accgccaggg ccaataacag aattgtcttg gaccaaactc caaaaatcat ttgcccaatg
     2041 ccgaacaaaa tggaataaac aagaataatc ccgagaatcc aattcagcac gttgggccaa
     2101 atctctccct gtcgctcgct aagtcctaca gctgtgacaa ccggacgcca accaggtccc
     2161 gccggttcaa cattttgata gaaactgatc aatttggaca tcggttccgg actggtggca
     2221 aaagtcacaa caatccatac aatcgtagta accgcgaccg tagtcagtat ttgttgcgcg
     2281 aatccaatcg gagttgacga atcaaagata ctaaactgca aaagcgttaa cgacacgatc
     2341 aacgcagacg ccatagcggc aatttcggac cacgcgttga tcctccacca ataccagcga
     2401 aggatataaa caagtccggt cccggcacca agcgcaagca cgagttccca gccactcgtg
     2461 atgcgattca tgaaatacgt taccacgatc gacaatagca cgataatcag tgtggcaact
     2521 ctggaagcaa atacataatg tttttcagtg gcttgcggct taacaaaacg cctgtagaag
     2581 tcattaatta cgtagcttgc accccagttc atttgagtcg agatcgtgct catgtacgcg
     2641 gccgcaaacg aagccaataa gaggcctcgg aatccaatcg gcaagtagtc gttcatgacc
     2701 tgtacatagc caaacgctgc atccggcttt ccggtttccg gatttagagt cgttccgttg
     2761 taaagaacta gcgagcaaag cgcaacaata atccagggcc atggccgcaa agcgtagtga
     2821 gcgatgttga accagagtgt ggcgagcaat ccatgttttt cgttctttgc tgaaaagata
     2881 cgttgggcga catacccacc accacctggt tcggcgcccg gataccatga tgcccaccaa
     2941 ttgacggcca gatataccga gagtgttaac aacggcaaca tccacaccgc agacccgtca
     3001 ggtaaaaggg cgattgcacc acccgggccg aaaggttcag ttgaaccgac gggcgtcact
     3061 ccggcaagcc gagctttcag attatcgagc ccgccaactg ctgctactgc aaaaaatgcg
     3121 agtgcgattg aaccagccat cgcaatcaca aactgaaccg cgtccgttac aaccactccc
     3181 caaaatccag acagtattga atagaggaaa gtaatgccga gacataaagc gagtgcttgc
     3241 cattttgcga gcccaagtgt tccgctgagg accttcgcca tccccagatt gacccagccc
     3301 ataatgatca ggttaatcgg aaacccaagg tagagcgctc taaatccacg cagaaaagcc
     3361 gccggtttgc ccgaataacg caactcgaca aactctacgt cggtcatcac cctggcccta
     3421 cgccatagcc gggcaaagaa gaagacggtc aacataccgc tcgccagcat cgaccaccat
     3481 agccagttac ccgcgactcc gtttcgggcc accatttccg tgacggcaag cggcgtgtct
     3541 gccgcaaatg tcgttgcgac cattgacgtt cccgcgagcc accacggcat tgaacgatca
     3601 gaaagaaagt attcttcagt tgaacgcccg gccctctttg cgtaatagag accgattgat
     3661 aaagagagaa caaaataggc agcaataatc aaccaatcga tactgactag tttcaacgtc
     3721 gatcctccaa tgcgagtgac gttcgataca aagggcagtt gccggattct ttttacttca
     3781 aactgagaag gcaggtatag ctgaaacgcg agtgatctca aggcatgaac gcctggttgt
     3841 caagtggggc tgggttttta tttcaaaatc gagctaaggg gtgaccgcca gggctcgg
//
LOCUS       DASSFP010000300         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_523100, whole genome shotgun sequence.
ACCESSION   DASSFP010000300 DASSFP010000000
VERSION     DASSFP010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523100"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /gene="dnaG"
                     /locus_tag="VFH91_06680"
     CDS             <1..1572
                     /gene="dnaG"
                     /locus_tag="VFH91_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET6862712.1"
                     /translation="AIKIVADKVGMPLPTMIEDPRFAALRRESDDVIQLNNWALEWWQ
                     QQLNTGGEARIAREYLSQREIGEETQQTFRLGYAPDSWEALSSYLRTKGASQLQIDRS
                     GLVVKKDEGGSYDRFRGRLIFPVFDAQGKPIAFGGRTLRNEDAKYINSPETPAYVKGR
                     NLFGLNLTRDEIRRNGFAILVEGFLDLIVPYQAGIRNIVASLGTALTADQVKLLNRFA
                     RKVVVNYDGDRAGIQAAKKSIELLLAEDLEVKVLVLPDNADPDEYIRKFGVTEYQQQR
                     AQAKPHIQFVIENALRDRSLNRPADKAEAVEEVLPYIRAVNGKLQKREYFDMAMNALQ
                     ITDVPLRRELWQTMRSPMASQAGEASKRVLKRSDVKPTVAERQLLEMLLADQELRHEV
                     LSRLEKSDYEFLATGAVFQAVIDLEAEGKEPDLEGLTEKLDDDLDAKELVPALLIGET
                     QWEEGDNAPSFRVLAEKCLDALRLVNIDRRIRELNAEIASAERTGDETKMVELISEYS
                     GLNRRRRSFEPQSQI"
     gene            1683..3413
                     /gene="rpoD"
                     /locus_tag="VFH91_06685"
     CDS             1683..3413
                     /gene="rpoD"
                     /locus_tag="VFH91_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET6862713.1"
                     /translation="MSIEDKDRDDVMERLLELGGDKKYLTFDDLNRELPDNVVSPEDI
                     EDVLQKLEGSNIPVADSDERLLEQAAAAATDDDEEEVDEDVELDLSAGALEKTNDPVR
                     LYLREMGVVPLLTREGEVAIAKRIERGQLKTQKAIARSPIAVRELLKVGDDLETNRAN
                     IRDIVTFSEQAELTGDEDKADEYRTWTIEGIQNINKLYKAGLKEFEKMLEEQKESKGK
                     KTRKLTRLRRAVARTRLEIAQEIGRLNLTERARQRLINSIRAVHKSVREAERDVDSCN
                     EKINRKRIKPDDAREFKRRILLAKKRLAEVEQDNHVGPAEIKRALQSIVTGEQQTGQA
                     KRELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEWRRGYKFSTYATW
                     WIRQAITRAIADQARTIRIPVHMIETINKLIRTSRALVQELGREPTSEEIARKMDIPV
                     SKVRKVLKIAQEPISLETPIGEEEDSHLGDFIEDKSILNPADAVVASNLREITDEVLA
                     TLTPREEKVIKMRFGLGTTGSEHTLEEVGQHFAVTRERIRQIEAKALRKLRHPSRSRK
                     LTAFLESAPH"
     gene            3484..4326
                     /locus_tag="VFH91_06690"
     CDS             3484..4326
                     /locus_tag="VFH91_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET6862714.1"
                     /translation="MSFNNLVSSLLQKSIGFRGGDRVVNYLAHHSAMLQCAPVKVGSL
                     KLFMDLRSPTCRDWLQQDLDTWEPGEREVMRQFVMPGDVVYDIGANMGLHTAFLSSLV
                     GPNGKVFTFEPNPALRKPLERTCENLTNVQFYPVGLGRERTVETLYVPADHTMASLRQ
                     WTAQTTREFPVTVHQLDGLDLPVPAFIKCDVEGAELEVFQGATRILSHRPVLLFEVNE
                     HCAKSFNRPATAVVDFLAGLNAYEFFVVGDTELVPMHESVRCANIVAIPTYRTDEEGK
                     SLRN"
     gene            complement(4335..4715)
                     /locus_tag="VFH91_06695"
     CDS             complement(4335..4715)
                     /locus_tag="VFH91_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862715.1"
                     /translation="MITHRKQERGGAQLKFLIVMTFIGATAYAGYMYVPIAYQAYAQK
                     DFMQHCVDVAVTQGYREQWVIDQLKKNAVEYNIPEDAQVTAVLRDNRVELTVQYIKPI
                     EFPGYTYNYEFDQTARSAAFLSIK"
     gene            complement(4715..>5186)
                     /locus_tag="VFH91_06700"
     CDS             complement(4715..>5186)
                     /locus_tag="VFH91_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862716.1"
                     /translation="CKFKTSIVANDERESTERTDSKSRRILNFGHTTAHALEKVTNYR
                     YFRHGEAVGYGMLVAGEISKHLGLLSSSKLDLLREAVNLCGNLPSAKKLNLNEIKAAL
                     KFDKKSVRGQINWVLLDDIGSPTIVEGQAISNKVLSLSLSEALGIAKRRTERSH"
BASE COUNT         1358 a         1221 c         1366 g         1241 t
ORIGIN      
        1 gcaatcaaga tagtagcgga taaagtggga atgccgctgc cgacgatgat tgaggatcct
       61 cgcttcgctg cgttacgtcg tgagtcagat gacgtaattc aactaaataa ctgggctctt
      121 gagtggtggc aacaacagct caacacaggt ggcgaagctc gtatcgcccg tgaatatctc
      181 tcgcagcggg agatcggcga agaaactcag cagacgtttc ggcttggata cgctcctgat
      241 agttgggagg ctttatcgag ctacttgcga accaaaggtg cgagtcaatt acagattgat
      301 cgcagcggtc tcgtcgtgaa gaaggatgaa ggaggttcgt acgatcggtt caggggacgt
      361 ttgatctttc ctgtttttga tgcgcaggga aaaccgattg ctttcggagg tcgaacgtta
      421 aggaacgagg atgcgaagta cataaactca cctgagacac cggcgtatgt gaagggtcgt
      481 aacctgtttg gtttgaatct cacgcgcgat gagatcagac gaaatggttt cgcaattctg
      541 gtagagggtt ttttggatct aattgttccc tatcaagctg gaatacgaaa cattgtagct
      601 agtctgggta ccgctctaac agccgatcaa gtgaaattat tgaaccggtt tgcgcgcaaa
      661 gtcgtggtca attatgatgg cgatcgtgca ggtatacagg cagctaaaaa atctatcgag
      721 cttctcctcg ccgaagacct cgaggtaaaa gttttagtac ttcccgacaa tgctgatccc
      781 gacgagtaca tccgaaagtt tggcgttacc gagtatcaac aacagagagc acaagcaaaa
      841 ccgcacatac aatttgtgat cgaaaacgct ttgcgcgatc gcagccttaa caggccagcc
      901 gataaggcgg aagctgttga ggaagttctt ccttacattc gtgccgttaa tggcaagctt
      961 cagaaacgcg agtatttcga catggcaatg aacgcactgc aaatcaccga cgttccactt
     1021 cgtcgtgaac tgtggcaaac gatgcgttct cctatggcgt cgcaagctgg ggaagccagt
     1081 aagcgagttc taaaacgaag tgatgtaaag ccaacggttg cggaacgcca attgttggaa
     1141 atgcttttgg cagatcagga actgcgtcat gaggtgctga gccgtttaga gaagtcagat
     1201 tacgaatttc tggccacggg tgccgttttt caggcagtga tcgaccttga agctgaaggt
     1261 aaagaaccag accttgaggg tctaactgaa aagctggatg acgaccttga cgcgaaagaa
     1321 ctagtcccgg cgctattgat tggcgaaaca cagtgggaag aaggagataa cgcgccttcg
     1381 ttcagagtat tggcagagaa gtgcctcgac gcattacgac ttgtaaatat cgatcgacgt
     1441 attcgtgaat tgaatgcaga aattgcttcc gccgaaagaa caggggatga aacaaaaatg
     1501 gttgagttga tctctgagta tagcgggctt aatcgcaggc gccgttcgtt cgaacctcag
     1561 agtcagatct agtgagttta tgaattgggt ttttggaata ggacctatag gacttatagg
     1621 acctatcgat taggacaaac aggcccgacc ccataacccc tggcccccgg agttttaacg
     1681 cagtgagtat tgaagacaag gatcgcgacg acgtaatgga acggctgctc gaattgggcg
     1741 gcgacaagaa gtacctgacc ttcgatgact tgaatcgaga gttacccgat aacgtcgttt
     1801 cgcctgaaga tattgaagac gttttacaaa agctcgaggg atcgaatatc cctgtcgcgg
     1861 attccgacga acgtctactc gaacaagcag cagcagcggc aactgacgac gatgaagaag
     1921 aggttgacga ggatgtagaa ctcgacctct cggccggagc actcgaaaaa acaaatgatc
     1981 ccgttcgcct ctatcttcgc gaaatgggcg ttgttccgtt gctaacacgt gaaggcgaag
     2041 ttgcaatcgc aaagcgcatt gaacggggtc aactcaaaac tcaaaaagcc atcgctcgat
     2101 ctcccatcgc agtcagagaa ctcctgaagg tcggcgacga cctcgaaacc aaccgggcca
     2161 acatccgcga tatcgtcacc ttctctgaac aggcggaatt gaccggtgac gaagacaagg
     2221 cggatgagta tcgtacttgg accatcgaag gaatccagaa catcaacaaa ctctacaaag
     2281 caggtctaaa agagtttgaa aagatgctgg aagagcaaaa ggaatctaaa ggtaagaaga
     2341 ccaggaaatt gacgcgcttg cgacgcgcgg tcgcgcgtac tcgactcgag attgcccagg
     2401 agatcggcag actgaacttg accgaacgtg cgcgtcagcg actgattaac tcaattaggg
     2461 ctgtgcacaa atcggttcgg gaagctgagc gggacgtcga cagctgtaac gagaaaatca
     2521 accgaaagcg catcaagcct gatgatgctc gcgagtttaa gcgtcggatc ctacttgcca
     2581 aaaagaggct tgcagaggtt gagcaggaca accatgtcgg accagctgaa attaagcgtg
     2641 ccctgcagtc cattgtcacc ggtgagcaac agacgggtca ggcaaaacgc gagttagtcg
     2701 aagctaactt gcggcttgtc gtatccatag ctaagaagta caccaaccgt ggtctgcagt
     2761 tcctggattt gattcaagag ggcaacattg ggttgatgaa ggccgtcgat aagttcgagt
     2821 ggcgtcgggg ttataagttt tcgacttatg ccacgtggtg gatcaggcag gcgataaccc
     2881 gagcgatcgc cgatcaagcc cggacgatac gtattccggt ccacatgatc gagacgatta
     2941 ataagttgat tcgaacctcg agagctcttg ttcaggagtt gggccgcgaa cctacaagcg
     3001 aagagattgc gcgtaagatg gatattcccg tctcgaaagt gcgaaaggtc ttaaagattg
     3061 cccaggagcc aatttcactc gagacgccaa tcggcgagga agaggactca caccttggcg
     3121 attttattga agacaagtcc attctgaatc ctgccgacgc cgtagttgcg tcaaacttga
     3181 gagaaataac cgacgaagtg ttggcgacgc tcactcctcg cgaggagaag gtgatcaaga
     3241 tgaggtttgg actcggaacc actggttccg agcatacgtt ggaagaggtg ggccagcatt
     3301 ttgcagtaac acgagagcgt attcgacaga tcgaagcaaa ggccctccgc aaacttcgcc
     3361 atccatcgcg gtcacgtaag ttgacagcgt tcctcgagag tgctcctcac tgattgttcc
     3421 attagttgta agaccgtgtt aggctgaggc gtcgacgcaa ctaattaaac atacgagcat
     3481 aggatgtctt tcaataacct cgtatcttca cttctccaaa aatcaatagg atttcgcgga
     3541 ggcgaccgtg ttgttaatta cctggcgcac cattctgcca tgcttcagtg tgcgccggta
     3601 aaagttggta gcctaaagct gttcatggac ctccgttctc ccacctgtcg cgactggctg
     3661 cagcaagatc tcgatacatg ggaaccggga gaacgcgaag taatgcgaca gtttgttatg
     3721 cctggcgatg tcgtctacga cataggggcg aacatgggcc tgcacactgc ctttctttct
     3781 tcgctggttg gacctaacgg gaaggtcttc actttcgaac cgaacccggc tttgcgcaaa
     3841 ccattagagc gaacttgcga gaacctcacg aatgttcagt tctatccggt tggacttggt
     3901 cgcgagcgta cggttgagac tctgtacgtg cccgctgacc acacgatggc cagcttgcga
     3961 cagtggacag ctcaaacgac gagggagttt cctgtgaccg tgcatcagct tgatggtctc
     4021 gatcttccgg taccagcttt tataaagtgc gacgttgaag gtgcagagct tgaagtcttc
     4081 cagggagcta caagaatact ttcccatcgt ccggtgctcc tgtttgaggt caacgagcac
     4141 tgcgcaaagt cgttcaatag acctgccaca gctgtagttg acttcctcgc ggggcttaac
     4201 gcttatgagt tttttgtggt gggtgacact gaacttgtgc ccatgcatga gagtgtccgc
     4261 tgcgcgaaca tcgttgccat tccaacttac cgaactgatg aggaaggaaa gtctctcagg
     4321 aattaaaccc tcggctattt gatgcttaga aatgcggcac ttcttgcagt ttgatcgaac
     4381 tcgtaattgt aagtgtagcc gggaaactcg attggcttaa tgtactgcac tgttagttcc
     4441 acgcggttat cgcgtagtac tgcagtgact tgggcatcct ctggaatgtt gtattcaacg
     4501 gcgttcttct tcaactgatc gatcacccac tgctctcgat aaccttgagt aaccgccacg
     4561 tcaacgcagt gctgcataaa gtccttttga gcgtaagcct gatacgcgat gggtacatac
     4621 atatatcccg cgtaggcagt tgcgccgatg aaggtcataa cgatcaaaaa ctttagctgt
     4681 gcaccacccc gttcctgctt tctatgcgtt atcatcagtg agacctttct gttcgtcgct
     4741 tcgcgatccc taaagcttct gatagcgaga gacttaaaac cttgttacta atggcctggc
     4801 cttcgactat cgttggtgag ccaatgtcat caagcagaac ccagttaatt tgtccgcgta
     4861 cactcttttt atcaaacttc aatgcggctt tgatttcgtt gaggttcagc tttttggctg
     4921 atggcaagtt gccgcagaga tttactgcct cgcgaagcag atctaatttt gaggaactca
     4981 gcaagcccaa atgtttcgat atttcgccgg ctactaacat tccataacca accgcctctc
     5041 catgtcggaa gtagcggtag ttcgtaactt tttccagcgc gtgcgcagtc gtatgaccaa
     5101 agttcaaaat acgtctcgac tttgagtccg ttctctccgt tgactctcgc tcgtcgttgg
     5161 caacaattga agttttgaac ttgcaa
//
LOCUS       DASSFP010000301         6617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_523170, whole genome shotgun sequence.
ACCESSION   DASSFP010000301 DASSFP010000000
VERSION     DASSFP010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523170"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="hemA"
                     /locus_tag="VFH91_06705"
     CDS             <1..870
                     /gene="hemA"
                     /locus_tag="VFH91_06705"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET6862717.1"
                     /translation="TAGRVLNRLIHHTFRVAKRVRSETGIAANAVSISYMAVELGKKI
                     FESLKGRTVMLIGAGEMAELSARHLVNAGASRVIITNRTEQAARELADEFGGETVHFD
                     EFPKFLGEADVVICSTGAQSFVLTEKMTREALDRRRNKPTCLIDISVPRNIEPSTGNI
                     PNVFLFDIDDLESVISSNIREREREAERAELIVQSEVMQFQQSLRLMDAGPTIGALRQ
                     KLQDTARAELQRQRKRLGQLSPEQETAIEQLLISTVNKISHPVLDQMRRFYESSEPDE
                     ISPQTDALNDEET"
     gene            873..1802
                     /gene="hemC"
                     /locus_tag="VFH91_06710"
     CDS             873..1802
                     /gene="hemC"
                     /locus_tag="VFH91_06710"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET6862718.1"
                     /translation="MKSALIIGSRGSKLALWQANHIKAKLTELRPDLEIRLEIIKTTG
                     DVRSDPLSVIGGQGVFTKELEESLLKAQIDLAVHSLKDLPTVLPDQLVIAAIGEREDP
                     RDALVLPANSNVAVSSLKDLPKQAVVGTSSQRRVAQVKSLRRDVEIKDVRGNVDTRLR
                     KLDEGQYDALILAAAGLTRLGLRDRISAYISPTEILPAVGQGALAIEARAQDHFTHEL
                     VAQLTHRPTELACLAERSLLRNLGGGCQLPIAGHATVDGPHLVLDGLVAMPDGTRILR
                     EQISGNPARAEELGRDLATLLTIQGAQSILKAQ"
     gene            1799..2602
                     /locus_tag="VFH91_06715"
     CDS             1799..2602
                     /locus_tag="VFH91_06715"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET6862719.1"
                     /translation="MTIPQALVGRTVVVTRAASQAGDLVAALERYGATVLSCPLIEIR
                     DPNDYARLDEAIDHLYGYDWIIFTSGNAVEFFLKRLSFKNVSYTELDEIKVAAIGDST
                     AEKLRDAYVHVDLVPSLSKSEGVLSALVDYVGGDEHLQGLNFLMPRATVGRDYLPKAL
                     EAANARVDVVATYQNVIPDNLDRGKFSAMLAGGADCIAFTSSSTVKNLALLFDTNDLS
                     EKLQGLTIACLGDVTAATAKRFGLEPTIQPWHSTMAEFAAAIASHFEEP"
     gene            2691..4073
                     /locus_tag="VFH91_06720"
     CDS             2691..4073
                     /locus_tag="VFH91_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862720.1"
                     /translation="MSTRSVLIFDKDTNSRSTISQLLSEWGFQSFIAEDLPSALDALT
                     KLNPTLVVDGGSALTSEDFALLREMRAKDIDVPVVLLTEHGSVEAAVNALQNEGVYHY
                     FQRPLDPIKLRLVLDRAVELKLAKQENEFLRRQLQDRGAFGELVGNSDSIRKVYTLIE
                     QVSPSSASVLITGESGTGKELVARTIHKMSPRRDRPFVAINCSAIPETLMESELFGHE
                     KGAFTGAASRRQGCFELADTGTLLLDEIGEMPAVLQAKLLRVIEERSVRRLGSRKEIE
                     VDVRLLAATNRHPQQAVAEGTLRSDLLYRLNVFGIHLPALAERKEDLPLLAQHLVTQL
                     AAKHGRPARFLSPSALNALEFHAWPGNVRELRNVIERAVIICSGEQIERHHFAPYPIE
                     QRERLRNEDTLSLPVGTPLEEVERQMIMRTLQKTKNNKTRAAELLGISLKTLHNKLNL
                     YRERGLLNDK"
     gene            4063..4968
                     /locus_tag="VFH91_06725"
     CDS             4063..4968
                     /locus_tag="VFH91_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862721.1"
                     /translation="MTSDASVLLFFIDGLGIGKRGPFNPLDNLEDSGPLAIFQNEPAH
                     VLLDGIVVPTDASLGVEGRPQSASGQTTILTGVNAPQLLGYHKQGFPNQALLKVIQEN
                     SIFKQLREAGVSPITFANAYTERFFVERPRWVSATTAAIEAADLTFRNVADLLRGEAV
                     FMDFTNKILIERGEQVTERTPAEAAQVLADITAINRFTLYEYFITDKVGHAQDMEKAK
                     VVLQQLACFIRELLARIDLTRTTVILTSDHGNIEDLSVRNHTLHKVPTIIWGAERESV
                     AVRIQSLVDITPAIVALLTTQIGSS"
     gene            4965..6353
                     /locus_tag="VFH91_06730"
     CDS             4965..6353
                     /locus_tag="VFH91_06730"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6862722.1"
                     /translation="MKTQKPNSEVAEVTFPKNDPRELFGWKMYDWANSAFYTTVVGAL
                     FSPYLTRLAQSAVGENGVVLNLGPLGAVTAKSLPTLCVSISVGAQVFLLPVLGALGDY
                     SNLKKRLMALFCYLAVFANCLLFFVKDDLYLLGGVLFIIANVGFGASIVFYNSFLSEI
                     TTEDQADKVSSRGFAYGYLGGALLLALNLILVLKAEAWGFSTGLAVRLSLLSAGIWWG
                     GFALITFVLLKSRPKSKVLPPGRSYLSAGFIEIFATVRELKRLPLTLRYLLGYLVYND
                     GIQTVIFAASAFLEQELFPGGNPIFLLEIFLMVQFVAVVGALLFERLAYVIKTKNAIV
                     VSLIIWAAIVIYAYRFLHTVPEAWILAAVIAIVLGGSQALSRSLFSRMIPAGKEAAFF
                     GLYEVSERGTSWMGPLLFSIVIARTGSYRQALLSLIFFFVVGLVVLWFTDTDRGIHEA
                     GNLVPEEVRHPS"
     gene            complement(6350..>6617)
                     /locus_tag="VFH91_06735"
     CDS             complement(6350..>6617)
                     /locus_tag="VFH91_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862723.1"
                     /translation="IAGLLFVFVYRRLRPYLQLMQKVVNALNQNSPARRDTSAQAERK
                     LVRCVNCGTWIPSDKAIRIGTTIYCSAECIEKQSANSNRKLSSR"
BASE COUNT         1598 a         1661 c         1739 g         1619 t
ORIGIN      
        1 actgcggggc gggtgctgaa ccgtctgatt catcacactt tcagggtcgc aaaacgagtt
       61 cgttcggaaa ccggcatcgc cgcaaatgcc gtttcaatca gctacatggc cgtggagctt
      121 ggtaagaaga ttttcgaatc actgaaaggt cggacagtga tgttgatcgg agccggcgaa
      181 atggctgaac tgtcagctcg acatctggtg aatgcgggag cgtcccgagt cataatcacg
      241 aatagaaccg aacaggccgc tcgggaattg gccgacgagt ttggcggcga aacggttcat
      301 ttcgatgaat tccctaagtt tctcggcgag gctgatgtgg taatttgctc cactggggca
      361 cagagttttg ttctaacgga gaaaatgacg cgtgaggcgc tggaccgtcg tcgaaataag
      421 ccaacttgtc tgatagatat cagcgtgccc agaaacattg agccctcgac cggcaacatt
      481 ccgaatgtgt tcctcttcga tattgatgac cttgagtccg taatctcttc gaatattaga
      541 gaacgagagc gagaagctga acgtgcagag ctgatcgtgc aatcagaagt gatgcagttc
      601 cagcagtcat tgcggttgat ggatgctggt ccaaccattg gggcgcttcg tcaaaagcta
      661 caggacacgg cccgtgcgga actgcaacgt caacgtaaac ggctcggtca attaagcccg
      721 gaacaggaaa cagcaatcga gcagttattg atttcgacgg tcaacaagat ttctcaccct
      781 gtgctggacc aaatgagacg cttttacgaa agcagcgagc cagacgaaat ttcacctcaa
      841 accgatgcgc tgaacgacga agagacttaa caatgaagtc agcgctaatc attggctccc
      901 gcggttcgaa actggctctc tggcaggcga atcacatcaa agctaaactc accgaacttc
      961 gacccgacct cgaaatacga ttagagatta ttaagactac cggcgacgtc agatcagatc
     1021 cgttgtcggt aatcggtggc cagggtgtct ttaccaagga gcttgaagag tcgttgctta
     1081 aggctcaaat tgatctggca gttcattcgc tgaaagacct gccgactgta cttccggatc
     1141 aactggtaat tgctgcaatc ggggaacgag aagatccgcg tgacgccctc gtgctcccag
     1201 ccaattcaaa cgttgctgtc agctcattga aggatcttcc aaagcaggcc gttgttggta
     1261 cgtcgagtca acgccgagtc gcacaagtga aatcgctgag acgcgacgta gaaatcaagg
     1321 atgtgcgagg aaatgtggat acccggctgc gaaagctcga cgaaggccag tacgacgctt
     1381 tgatcctggc ggcggccggt ctcaccagac ttggcctgcg agatcgaatc agcgcttaca
     1441 tctcacccac tgagatccta ccggcggtgg gtcagggagc tctggctatc gaagcaaggg
     1501 ctcaggatca cttcacccac gaactggtgg cccaactaac tcatcgtccg accgaactcg
     1561 cctgtttggc cgaacgatcc ctgttaagaa atcttggtgg tggttgtcaa ttacctatag
     1621 ctggtcacgc aacagttgat gggccacact tggttcttga cggactggtt gcgatgccgg
     1681 acggtacccg gatccttcgt gaacaaatct ccggaaatcc cgcgcgcgca gaagagcttg
     1741 gccgagattt agccacgcta cttacaattc aaggtgctca gtcgatttta aaagcacaat
     1801 gacaatccct caggctctag ttggacgaac tgtagtcgta actcgtgccg cttctcaggc
     1861 aggtgatctg gttgccgcac tcgagcgata tggcgccacc gtgctctcat gtccgttgat
     1921 tgagattcga gatccaaacg attacgcacg cctcgacgag gcgatcgatc atctgtacgg
     1981 ctacgactgg ataatcttca cgagtggtaa cgcggtagag ttctttctga aacgcctctc
     2041 gttcaaaaat gtgagctaca ctgaactgga cgagattaaa gttgcggcaa ttggagatag
     2101 cactgccgag aagttgcgag acgcatatgt gcatgttgac ctggtaccct ccctatcaaa
     2161 atcagagggg gttttgtctg cgctcgtcga ttacgtcggc ggagacgaac acttgcaggg
     2221 actaaacttc ttaatgcctc gagcgaccgt tggccgcgat tatctgccga aggccttaga
     2281 agcggcaaac gcgcgcgtcg atgttgttgc tacatatcaa aatgtaattc ccgataatct
     2341 cgaccgcgga aagttcagtg cgatgctggc tggcggggcc gactgcatag catttaccag
     2401 ttcctcaacg gttaagaatc ttgccctgct atttgataca aacgatttga gtgagaagtt
     2461 gcagggtctt acgatcgctt gccttggtga tgttaccgcc gcgacggcaa agcgatttgg
     2521 gcttgagcct acgatccaac cttggcattc cacaatggct gaattcgcag cagcgattgc
     2581 gagtcatttc gaagagccat gacgtctacg aaactgacaa aaattgtcgc atcgtcggct
     2641 aagatcacct actgaaaatc attcgccgta gctgtgctac actgctcccg atgtctaccc
     2701 gaagcgtact catcttcgac aaagatacaa acagtcgatc aaccatcagc cagcttctgt
     2761 cggaatgggg ctttcaatca ttcatcgcgg aagatctgcc gtcggccctg gatgcactga
     2821 cgaaactcaa tccgacgctt gtagtggatg gtgggtcagc gctgacttct gaggactttg
     2881 ccctcttgcg cgagatgcgc gcaaaagaca tcgacgttcc ggtcgtattg ctcactgaac
     2941 acggatcggt ggaagcagct gtcaacgctt tacagaacga aggtgtctac cattattttc
     3001 aacgtccgtt ggacccgatc aagttgcgac tggtgctgga ccgcgctgtc gaactgaagc
     3061 tcgcaaagca agagaacgag tttttgcgga ggcagcttca ggaccgtgga gccttcggtg
     3121 aattggtggg caattcagat tctattcgca aggtctacac tttgattgaa caagtgtcgc
     3181 cgtcatccgc gtccgttctg ataacgggtg agtccggcac tggtaaggaa ctcgttgccc
     3241 gaaccattca caaaatgagc ccgcgccggg accgtccgtt tgtcgcgatt aattgttccg
     3301 cgattccgga aaccctgatg gagtcggaac tgttcggaca cgaaaaagga gcatttactg
     3361 gcgctgcgtc aagaagacaa ggatgttttg aactggcaga cacgggaact ctcttattgg
     3421 atgaaattgg cgaaatgccg gctgtgcttc aagcaaaact gctgcgcgta attgaagagc
     3481 ggtcagtgcg gcgattaggc agccgaaagg aaatcgaagt agacgtacgt ctgcttgctg
     3541 ccaccaaccg gcatccacaa caagctgtcg ccgaaggtac tttgcgcagc gatttgctat
     3601 atcgtttgaa cgtgtttgga attcatctgc ccgctctcgc ggaacgcaag gaagacctac
     3661 cgcttttggc ccagcacctg gtaacccaac tcgcggcaaa gcacggccga ccagctcgtt
     3721 tcttaagtcc atcagctttg aacgcactcg agtttcatgc gtggccaggt aacgttcgcg
     3781 aactccgaaa tgtgattgag cgggccgtga tcatttgctc cggggaacaa attgagcgac
     3841 atcatttcgc tccgtacccg atagaacagc gtgaacgttt gcgaaatgaa gacacgttat
     3901 cgcttccagt tggtacgccg ctcgaggaag ttgaacggca gatgattatg cgaacgctcc
     3961 agaagacgaa gaacaataag acgcgtgcgg cagaacttct tggcataagc ttgaagacac
     4021 tccacaacaa acttaatctt tatcgcgaac gcggcctgct taatgacaag tgacgcgtca
     4081 gtcctgcttt tctttattga tggccttggt atcggcaagc gcgggccatt caatccactc
     4141 gacaatctgg aagattctgg tccactggcc atttttcaga atgagccagc ccacgtattg
     4201 ttagatggta tcgtagttcc caccgacgcc agtctcggag tcgaaggacg cccccaaagt
     4261 gcgtccggcc agaccacaat tcttacgggc gtaaatgcgc cacagttgct cggttaccac
     4321 aaacagggat ttcctaatca agcactcctg aaagtcattc aagagaattc gattttcaag
     4381 cagttacggg aggcgggagt gtcgccgata acgtttgcga atgcttacac ggaacgtttc
     4441 tttgtggaac gtccgcgttg ggtctcagca accacggcag caattgaggc tgctgatctg
     4501 acttttcgta acgtcgccga tctgctgcga ggtgaagctg tatttatgga cttcaccaac
     4561 aaaatcctga tcgagcgagg agagcaagtt accgagcgga ccccggccga ggcagcgcaa
     4621 gtactcgcag atattacggc gataaatcga tttacgcttt acgagtactt catcactgat
     4681 aaggtgggcc atgcgcagga tatggagaaa gcaaaggtag ttttgcaaca gttggcgtgc
     4741 tttattcgcg aactgctggc acgaatcgac ctgactcgca caacagttat cttgaccagc
     4801 gatcacggca acatcgaaga cctctcggta cgtaaccaca cgctgcataa agttccaacc
     4861 atcatctggg gcgccgagag agaatcagtt gccgttcgca ttcaatcgct cgttgatatt
     4921 actcccgcaa ttgtcgctct tctgacaacg caaattggaa gttcatgaag acacaaaaac
     4981 cgaatagtga agtcgcagaa gttacctttc caaaaaacga cccacgcgaa ctattcgggt
     5041 ggaaaatgta cgactgggcc aactccgcct tctacacaac cgtcgtcggt gcgctcttca
     5101 gcccctacct gacacggttg gcccagtctg cagtaggtga gaacggcgta gtgctaaatc
     5161 tgggaccact gggcgcggtg accgccaagt ctttacccac tctctgtgtg tcgatttcgg
     5221 taggcgccca ggtctttcta ttgccggtac tcggcgcgct tggcgattat tcgaatctca
     5281 agaagcgctt gatggcgttg ttctgctact tagcagtatt tgccaactgt ttgttgttct
     5341 tcgtcaaaga cgatctgtat cttttgggag gagttctttt catcatagcc aacgtcggat
     5401 ttggcgcgtc gattgttttc tacaactctt tcctttccga aatcacaacg gaagatcagg
     5461 ccgacaaggt ttcgagccga ggttttgctt acggctacct tgggggcgca ctgttgctcg
     5521 cgctaaacct catattggtg ttgaaggccg aagcctgggg tttttcgaca ggactggcgg
     5581 tccgtttgtc tttactgtca gctgggattt ggtggggtgg ctttgccttg atcacttttg
     5641 ttcttttgaa gtcacggcca aagtcgaaag ttttgccgcc cggtcgcagt tatttatcag
     5701 caggttttat tgaaatattc gcgacggttc gtgagctaaa gagacttccg ctaacgcttc
     5761 gctatctgct tggatatctt gtttataacg atggaattca aacggttatc tttgcagcca
     5821 gcgcctttct ggaacaggaa ctctttcccg gtggcaatcc gattttcctg ttggaaatct
     5881 tcctgatggt ccagttcgta gccgttgtcg gggcattgtt gtttgagcgt ttggcctacg
     5941 tcatcaaaac taaaaacgcg atcgttgttt ccctgataat ctgggctgcg atcgttatat
     6001 atgcgtaccg gttcctgcac accgttcccg aagcctggat cctcgccgcg gttattgcga
     6061 ttgttctggg aggctctcaa gcactttccc gatcactgtt ttctcggatg attccggccg
     6121 gaaaggaagc tgccttcttt ggcttatatg aagtatccga gagaggtact tcatggatgg
     6181 gtccattatt gttcagtatc gtgatcgcaa gaactggatc atatcggcag gctctgttgt
     6241 cgttgatctt tttcttcgtg gttggattgg tcgtgttatg gtttacagac acagaccgtg
     6301 gaattcacga ggcaggaaat ctagtgccgg aagaagtgcg acacccaagc taacgtgagg
     6361 atagctttct attgctgttg gcggattgct tttcaataca ctcagctgag cagtagatgg
     6421 tggtcccaat cctgatggcc ttgtccgaag gaatccatgt accacaattc acacaacgta
     6481 ccagcttccg ctcagcctga gcagaagtgt cacgccgcgc gggagagttc tgatttaacg
     6541 cgttaacgac tttctgcatc agctgcaaat acggacgcag gcgccgataa acaaatacga
     6601 ataacagccc tgcgata
//
LOCUS       DASSFP010000302         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1855894, whole genome shotgun sequence.
ACCESSION   DASSFP010000302 DASSFP010000000
VERSION     DASSFP010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855894"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFH91_06740"
     CDS             <1..516
                     /locus_tag="VFH91_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HET6862724.1"
                     /translation="ITWKYPSCILAGDNSIGEFYSVALTNHAQQADTGTKMIHLGKNT
                     RSTIISKGIAAGKSNNSYRGLVKVMPKAENARNYTQCDSMLIGSSCGANTFPYIEVMN
                     SSSSVEHEASTSKISEEQLFYLQQRGISQEDAVSLIINGFCKDVFLQLPMEFAVEAQR
                     LLGLKLEGSVG"
     gene            616..1392
                     /gene="sufC"
                     /locus_tag="VFH91_06745"
     CDS             616..1392
                     /gene="sufC"
                     /locus_tag="VFH91_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET6862725.1"
                     /translation="MLEIRNLHAGIDGTEILKGIDLTIKKGEVHAIMGPNGSGKSTLA
                     KVLAGHPQYQVTAGEVLYNGQNLLELPPHERALEGVFMAFQYPIEVPGVSNAQFLRLA
                     YNEKQKHLGGEELDPLEFKDLLKERAKIVEMDASFMTRSVNEGFSGGEKKRNEILQMA
                     VLEPKLAVLDETDSGLDIDALRIVAGGVNQLRTAENAIIVVTHYQRLLNYIIPDYVHV
                     LASGRIAREGGKELALELEEKGYDWIRTEQNGSGVSAIAK"
     gene            1395..2723
                     /gene="sufD"
                     /locus_tag="VFH91_06750"
     CDS             1395..2723
                     /gene="sufD"
                     /locus_tag="VFH91_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET6862726.1"
                     /translation="MVSQIVKSDRNYQAAFLSLRETDPTAAWVQLVRDTAMDRFESLG
                     FPSVADEEWKYTNLAPWAKSSFTPASNQSNLTRDAVKSLRSAETTNSHLVLVNGFLRE
                     DLSSKSGLDEVVAVDLFSAIGDARYSKIVREYLARNADYQDSGLVSLNTAFLRSGVFI
                     LIPKGVKLKQPIQISYVSDGVRDNEVSFPRLLLVGEENSSASLIETFISTGDAPYFTN
                     AVAEIVLNDGAQLEHYRVQRDSINSLHIGKTATELGRGSRYDSTAITLGARLSRHDIS
                     VVLDHEGAECRVDGLYVVSSDQHADTHSVIDHKQPHCNSHQLYKGILDGNGRAVFNGK
                     IFVREGAQKTDAIQTNKNLLLSDRARVDTKPQLEIYADDVKCAHGAAVGQISEEELFY
                     LEARGIEPSVGRNLLTYGFAEEVIGKIKVDSIRTAVDEAVLRQLKTVLVS"
     gene            2751..>3169
                     /locus_tag="VFH91_06755"
     CDS             2751..>3169
                     /locus_tag="VFH91_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6862727.1"
                     /translation="MSSSVDLMTEKEAAWDVERIRADFPVLHQTVNGKPLVYLDNGAS
                     SQVPQVVIDRGSKYLEQEHSNIHRGVHYLSQKATTAYEGAREKVKRFINARESRECIF
                     VRGATEGVNLIMYGYGRKFVNSGDEIIISAMEHHANIV"
BASE COUNT          860 a          687 c          860 g          762 t
ORIGIN      
        1 attacctgga aatacccttc ctgcatactg gcaggcgata actcaattgg cgagttctat
       61 tcagtggcgt tgacgaatca tgcgcaacag gccgatacgg gcacaaagat gatccatctc
      121 ggcaagaaca ctcgttcaac aataatttca aaaggtattg ccgcgggcaa atcgaataat
      181 agctatcgag ggctggttaa agtcatgccc aaggctgaaa acgcgcgcaa ctacactcaa
      241 tgcgattcga tgttgatcgg ctcgagttgc ggggcgaaca cctttcccta tatcgaagtg
      301 atgaacagta gctcgagtgt tgagcacgag gcatcgactt ctaaaatcag cgaagagcag
      361 ttgttttatt tgcagcagag aggaatctcg caggaagatg cggtctcgct gattattaac
      421 gggttctgta aagatgtttt ccttcagttg ccgatggagt tcgcagttga agcgcaacgc
      481 ttactgggac tgaagctcga aggaagtgtt ggataaagat aaggcagcaa tatgtcgggt
      541 gtcagtggcg caattggttt gatatggcgt ctgacacccg atagctctga ccaggtttag
      601 gagaagagat tagtgatgct ggaaattcga aatttacacg ctggaattga tggaactgag
      661 attctcaagg ggatcgatct gaccatcaaa aaaggtgaag ttcacgccat catgggaccc
      721 aatggttccg gtaaatcgac gctcgccaaa gtgctggcgg gtcacccaca gtatcaagtc
      781 actgccggtg aagtgctcta taacggtcaa aacttgcttg agttaccccc acatgaacga
      841 gcgctcgaag gggttttcat ggcttttcaa tacccaattg aagttcctgg cgtttcgaat
      901 gctcaatttc tcaggttggc ttataacgag aagcagaaac atctcggcgg ggaagagctg
      961 gatccgctcg aattcaaaga tttgctgaaa gagcgcgcca agattgtcga gatggatgcg
     1021 agttttatga cgcgctcggt gaacgaaggg ttctcaggcg gagaaaagaa gcgcaatgag
     1081 attttgcaga tggctgtcct ggaaccaaag ctggcggtgc tggacgaaac agattccgga
     1141 ttggacattg atgctctgcg tatcgttgcc ggtggtgtaa atcagttgcg cacggcagaa
     1201 aacgcaataa tcgtggttac tcactaccag cgtctgttga attacatcat tcctgattac
     1261 gttcacgttc tggcaagcgg tcggattgca cgagaaggtg gaaaagaact cgctctcgaa
     1321 ttagaagaga agggttacga ctggattaga actgaacaaa atggaagtgg agtaagcgca
     1381 atcgcgaagt agctatggtg agtcaaatag ttaagtcaga tagaaactat caagctgcgt
     1441 tcctatcgtt gcgtgagacc gatccgactg cagcgtgggt ccaactcgtt cgcgacacgg
     1501 cgatggaccg ctttgaatcg ttgggctttc cctcagtcgc agacgaggaa tggaagtaca
     1561 caaatctcgc gccctgggcc aaaagcagtt ttacgcctgc ttcgaatcaa tcgaacctga
     1621 ctcgagacgc tgttaagagt ctaaggtcgg ccgaaacaac aaacagtcat ttagtgttag
     1681 ttaacggttt tttgcgcgaa gatctttcat caaagagtgg tttagatgaa gtcgttgccg
     1741 tcgatctgtt tagtgcgatc ggtgacgccc gatacagcaa gatcgttcgc gagtatctcg
     1801 caagaaacgc ggattatcag gacagcggac tcgtttcgct taacactgct ttccttcgct
     1861 ccggcgtgtt cattttgatt cctaagggtg taaagctgaa acagccaatt cagatctcat
     1921 atgtatcgga tggtgttcgt gacaacgaag tgagttttcc acgcttgttg ttggtgggtg
     1981 aagagaatag ttccgcaagt ttgatagaga ctttcatctc taccggtgac gctccgtact
     2041 tcacgaacgc tgtcgcagag atagtcctga acgacggggc acagcttgaa cattaccgtg
     2101 ttcagcgcga cagcataaac tcgcttcata tcggaaaaac ggccaccgag cttggacgag
     2161 ggagcaggta tgattccact gcgataacgc ttggagcgcg attatcgcga cacgatattt
     2221 cggtagttct ggaccatgaa ggtgctgagt gccgggtgga tggcctttat gtcgtgagct
     2281 cagatcagca cgctgacaca cactcggtta ttgaccataa acagcctcac tgcaacagtc
     2341 atcagctcta taaaggaata ctggacggta atggccgagc ggttttcaat ggaaaaatct
     2401 ttgttcgtga aggtgcgcaa aaaactgatg cgatacagac gaacaagaac ctcctgctct
     2461 ctgatcgcgc cagagttgat accaagccgc agctggagat ttatgcggac gatgtgaaat
     2521 gtgcgcacgg agctgcggtg ggtcaaatca gcgaagaaga actcttttat ttggaggcgc
     2581 gaggtattga gccgtcagtc ggtcgaaacc tgcttactta cggattcgca gaagaggtta
     2641 ttgggaagat caaggtagat tcaattagaa cagccgttga cgaagcggta ctaagacaac
     2701 ttaaaacagt cttagttagt tgatagtccg aactagacat aaggcgtcat atgagttcaa
     2761 gtgtagatct aatgaccgaa aaggaagcgg cttgggacgt cgagcgcatt cgtgctgatt
     2821 ttccagtgct ccaccaaact gttaatggca agccgctggt ctatctcgac aatggtgcgt
     2881 cgtctcaggt tccgcaagtt gttatcgacc gcggtagtaa gtatctggaa caggaacatt
     2941 caaacattca tcgaggagtt cattatctaa gtcaaaaagc gacaacggca tatgaaggcg
     3001 cgcgtgaaaa ggtcaagcgc ttcataaacg ctcgcgagtc gagagagtgc atcttcgtca
     3061 ggggcgccac cgaaggcgtc aatctcatca tgtacgggta cggccgaaag ttcgtgaata
     3121 gcggagatga gatcatcatt tcggcgatgg agcatcacgc caatatcgt
//
LOCUS       DASSFP010000303         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1571020, whole genome shotgun sequence.
ACCESSION   DASSFP010000303 DASSFP010000000
VERSION     DASSFP010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571020"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VFH91_06760"
     CDS             complement(<1..1631)
                     /locus_tag="VFH91_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET6862728.1"
                     /translation="MSRSRGEKKDSAEHLNVIGKPFRKVDARAKCVGQTKFADDVVLP
                     RMLFCKILRSHLPHALIKKIDATKALALPGVFAVITGKDLPIPYGILPVSQDEHALCI
                     DKVRFIGDPVAAVAAIDEDVAFDAMNLIEVEYEPLQTISSIEEAVMIDEPRIHEYGDS
                     GNIHKKVSLEFGDVEEGFAEADLIREDVFFYEGNTHLPMEQHAALAHFDSDEKITLWS
                     ATQTPHYVHRALAKVLGLAASHIRVIATPNGGGFGGKSDPFNHEVIVCKLAMLTGRPV
                     KVTLTREEVFYCHRGRHPVLMKIKTGVKNDGAITGMHFQTYLDGGAYGSYGVASTFYT
                     GALQTVTYDVPKYKFQGLRAFTNKPPCGPKRGHGTPQPRYGLEVHLDKIAEQLKLDPA
                     QMRRQHLVAPNTTTANYLRIGSMGLGACIDKVVAGSDWKNRFSNWDQGRRKLPFGKGI
                     GIACSSYICGAGLPIYWNNMPQSGVQLRLDRQGGVCVMCGSTDIGQGSDSILAYIVAE
                     ILGIDPFDIRVITADTDLTPVDLGSYSSRVTLMTG"
     gene            complement(1641..>2135)
                     /locus_tag="VFH91_06765"
     CDS             complement(1641..>2135)
                     /locus_tag="VFH91_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6862729.1"
                     /translation="FTLNDEQVEVAFAPHKTLLEVLREDLNLTGTKHGCELGECGTCA
                     ILVDGRSILSCLMLGLEAEGRSVKTIEGMAVGAELHPLQQTFADLGAAQCGYCSPGFL
                     LVAEELLERNPNPSRPEIQEALSGNLCRCTGYIKIYEAVELAAARMRGEEAELPRESV
                     YGLE"
BASE COUNT          509 a          577 c          507 g          542 t
ORIGIN      
        1 tttcccgtca tcagcgtaac tcggcttgaa tagctgccga gatcaacggg agtcagatct
       61 gtatctgccg tgatgacccg aatgtcaaac ggatcgatac caagaatttc agcgactatg
      121 tatgcgagga tggaatcgga accctgtcca atatcggtcg agccacacat gacgcagacg
      181 ccgccttgcc gatccagtct taattgcaca ccagactgag gcatgttgtt ccagtaaatg
      241 ggtaatccgg cgccacaaat gtacgagcta caggcaatcc caatcccttt accgaatggc
      301 agtttccgtc gcccctggtc ccagtttgaa aatcgatttt tccagtcaga accggcgacg
      361 actttatcaa tacatgcacc caggcccatg ctgccgattc gcaagtaatt agcggtagtt
      421 gtattcggag cgaccaggtg ttgccgtctc atctgggccg ggtccaactt aagctgctcg
      481 gcaatcttgt cgagatgaac ttctagtccg taacgcggtt gaggcgttcc gtggccgcgc
      541 tttggaccgc atggaggctt gtttgtgaag gcccgtaagc cctggaattt atatttcggt
      601 acatcgtatg taacagtttg caatgctccg gtgtagaacg tgctcgcgac tccgtaacta
      661 ccataagcac caccatcgag atatgtctgg aagtgcattc ccgtaatcgc accgtcgttc
      721 tttactccgg tcttgatttt catcaaaaca ggatgtcgtc cgcgatggca ataaaacact
      781 tcttcgcgag tcagcgtgac ttttaccggt cggccggtaa gcatcgccaa tttgcaaaca
      841 atcacttcat gattgaaagg atcactctta cctccaaagc cgccgccatt cggggtagca
      901 atgacgcgaa tatgacttgc ggccaggccc agcactttgg cgagcgcccg atgaacgtag
      961 tgcggcgttt gagtcgcact ccagagagta attttttcgt ccgaatcaaa gtgggccaaa
     1021 gcggcgtgtt gctccattgg aaggtgagta ttgccttcgt agaagaatac gtcttcgcga
     1081 attagatcgg cctcagcaaa cccttcttcc acatcgccga actcgagcga cactttctta
     1141 tggatgttgc cggaatcgcc atactcatga atccgaggct cgtcgatcat cactgcttct
     1201 tcgatcgagg aaatggtctg tagcggctcg tattcgacct cgatcaggtt catcgcatcg
     1261 aaagcaacgt cttcatcaat agcagcaacc gctgcgacag gatcaccaat aaagcgcacc
     1321 ttatctatgc agagtgcgtg ttcgtcctga ctgacgggca gaattccgta aggtatgggt
     1381 agatccttac cagtaattac agcgaatacg cccggtagcg ccaacgcttt cgtcgcgtca
     1441 attttcttga tcaaagcgtg aggcagatga ctgcgaagaa tcttgcagaa caacatacgt
     1501 ggtaacacta catcgtcggc aaactttgtt tggcccacgc actttgcgcg cgcgtccacc
     1561 ttccggaacg gtttgccgat cacattcaaa tgctcggccg aatctttttt ttctccgcga
     1621 cttctgctca tttatgttgt ttactcgagt ccgtacacac tctctcttgg gagttcagct
     1681 tcctcgcctc tcatccgtgc tgcggctaac tcaacggcct catagatttt tatataaccg
     1741 gtacaccgac acagattgcc tgataatgcc tcttgaattt cgggtcgaga agggtttgga
     1801 ttcctttcca gtagttcttc agccacaagc aagaatcccg gcgaacagta gccacattga
     1861 gctgcgccca gatcggcaaa tgtttgttgt agcggatgca actcagcacc gacagccata
     1921 ccttcgatcg tcttgacgct gcgaccttca gcctccagac cgagcattaa gcaagacaaa
     1981 attgagcgac cgtcaacgag tattgcgcag gttccgcact cgccaagttc acaaccatgt
     2041 tttgttcctg tcagattgag atcttcgcgc aaaacttcaa gaagagtctt atgcggagcg
     2101 aacgcaactt ctacttgctc gtcattaagt gtgaa
//
LOCUS       DASSFP010000304         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_229436, whole genome shotgun sequence.
ACCESSION   DASSFP010000304 DASSFP010000000
VERSION     DASSFP010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229436"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFH91_06770"
     CDS             complement(<1..389)
                     /locus_tag="VFH91_06770"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HET6862730.1"
                     /translation="MSTSEPIPGTTASAEVVVGPHHLASSLPLDQTDHFPAVFATAGM
                     IALMEIAGSRLLKPFLGPGELSVGVIVDVSHTAPTPPGALVTATARYTGREGKLFVFE
                     VSASDPGGEIGRGIHKRAIVNSERLART"
     gene            846..1334
                     /locus_tag="VFH91_06775"
     CDS             846..1334
                     /locus_tag="VFH91_06775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6862731.1"
                     /translation="MTTRERSFQWQDPIQLAEAAKDLSGLSFLRKIAAGELPPPPIGV
                     MMNFGLDQVEEGRVVFTVEPAEYHYNPIGVVHGGVAATLLDSAMGCAVHSKLAAGMFY
                     TTLEIKVNFVRAITRDAGKLYCEANLIHFGSRSATAEGRIIDSSQRLYAHGTTTCLIQ
                     RP"
     gene            1345..2448
                     /locus_tag="VFH91_06780"
     CDS             1345..2448
                     /locus_tag="VFH91_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6862732.1"
                     /translation="MNVNVAILWEQGQPLSVEEAELTAPGPGEVLLEIKAAGVCHSDL
                     HPVRGDWRTRTPLALGHEGAGIVCGLGDGVESVHVGDHVVLCWAPPCGVCVSCKLGRP
                     VLCDRLERTTFRNRLPSGGTRLRARNQDILHFNSTACFADFAVVSEQGIVKVPPHIPF
                     SSLATLGCAVITGMGAVTNVAKLSGRERIVVVGAGGVGLNVVQGAKIAGCDEIIAVDA
                     KPNPLHLALKFGATHTIDSSTQDVSDIVLQLTEGRGADFVFDTVGAPATVEQSFNLAR
                     KGGSVVVTGLARTDSLASIPIFPLVMQEKRFIGSVYGSGDPLKDINKLVKLYEQGVLK
                     LEELVARDYRLVQVNEALSALAAGEGARGIILW"
     gene            2479..3924
                     /locus_tag="VFH91_06785"
     CDS             2479..3924
                     /locus_tag="VFH91_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6862733.1"
                     /translation="MRIIANLLLVSICSAIALAADDWSQFRGPNGTGVSATTGLPVEF
                     GPSKNVLWKTELPPGHSSPVLAGKKLFVTAHTKEKETYKLLVFCLDRQTGKILWQREV
                     ARAQKGRLENVNSPASPSPVTDGTNVYVFFQEFGLVSFDGAGKERWKLPLGPFNMFYG
                     FGASPILVDDKVILPVDQDNPASYLLAIDKNSGKVRWKVDRPGVISGYSTPIIYQPKD
                     GPKQIVVPESFQLSAYSVVDGKRVWWVRGLACEMKSIASQDGDYLYINGWGFPTNQPG
                     EQVKTVPFDAALTRYDKNGDRMIEKAEATGGESMDKWLNLGFEAFDMNRDQKLDSPDW
                     EIFRSMMASENGLLAIKMGGTGDQTASAIRWRYQKPVPQVPSTLLYKGVLYMINDSGI
                     LISFDPATGNVIKQGRLRGAIDKYFSSPVAADDKVFLIGEGGAVSVLKAAGDWQVLAV
                     NELDDECFATPAIADGRIYIRTRSALYCFGA"
     gene            complement(4309..>5085)
                     /locus_tag="VFH91_06790"
     CDS             complement(4309..>5085)
                     /locus_tag="VFH91_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6862734.1"
                     /translation="GRQFSESENREVKHVVLINEAFARKYFADEDPIGKRIDVSMFDG
                     PNPTEIIGVVGNVRYESLIDGFEPAVYFPHPELAYSFMTFVIRTDGDPVAIAPALQRE
                     IRSLDPNQPVSDVRTMDQVMSDTFSRARFNTLLLALFAGLATLLSAVGIFGVMNYSVA
                     LRTHEIGLRVAIGAQPRQVLLLILRQGLLLTVVGVAIGLVAAFALTRLLSGLLFGVAA
                     ADPSTFATISILLVTVSLVACYLPARRAMKIDPLMALRSE"
BASE COUNT         1220 a         1324 c         1346 g         1195 t
ORIGIN      
        1 gtgcgcgcaa gccgttcact attgacgatc gctcgtttgt ggatgcctct acctatctca
       61 ccacccggat cactcgccga gacctcgaat acaaagagct tgccctcacg accggtgtaa
      121 cgagccgttg cggttactaa agcgccgggc ggagtaggag cagtgtgcga gacatcaacg
      181 attaccccaa cagataactc acctggtcca agaaagggtt tcaagagcct tgagcccgct
      241 atttccatta gagcaatcat ccctgcggtg gcgaacactg caggaaagtg gtccgtttgg
      301 tccagaggta gagagcttgc caaatggtgt ggtccaacga caacctctgc gcttgcggtt
      361 gttccgggaa tgggttcact ggttgacatg gttgatttga cctttggcct tagggctttg
      421 gcctttgtgt ttgttgctct gagctcttgc gttgtgttat tgggctcggg cgtgtaaccc
      481 cggcaagtct gaataacatc aaaagccgtc gatgtcgagg ctgatggaga gtcagtgcat
      541 cacctgccgc cccggcagag cagcacggca gtgtgtggga ttagttagca cctggcgaga
      601 gctcgcattg taggaaggac ttgaacgacc cacacagccg tgatgtggga ttatgagacc
      661 tggttccttc ttcggaattg aagcggcagg cgacatacta cttcctgatt ctgaagctcg
      721 gatcgcgcca acaacattcg atgaatagcc gaggagacag aaggcggtta gcaaagaggc
      781 cgccggctag cgttttgtgg tttgtggttt gcgttttgta ctttgagctt tgtaactttg
      841 tacacatgac aacacgcgaa cgctcatttc agtggcaaga tccaattcaa ctagccgaag
      901 cagccaaaga tttgtcaggc ctgtcttttc tcagaaaaat agctgccgga gaacttcctc
      961 ctccaccaat cggcgtaatg atgaactttg gcctggacca agtggaggaa ggccgcgttg
     1021 tgttcacggt cgagcccgct gaatatcatt acaacccgat cggcgtcgtg cacggcggtg
     1081 ttgctgcaac attgctggat tcagcaatgg ggtgcgctgt tcattccaaa cttgcagctg
     1141 gaatgttcta cacgacattg gagataaaag ttaatttcgt tcgtgctatt accagagacg
     1201 cgggaaaact gtattgtgag gcgaacctga tccattttgg atctcgatca gccactgccg
     1261 aaggtcgcat catcgattca agtcaacgac tctacgcgca tggcactact acctgtttaa
     1321 ttcaacgtcc gtgaatttca tgacatgaac gtaaatgtcg cgattctatg ggagcaaggt
     1381 cagccactct ccgttgagga agcggaacta actgcgcctg gtccaggcga agtgctgctc
     1441 gaaatcaaag ccgctggcgt ttgtcacagc gacctccatc cagttcgcgg agactggcga
     1501 acccgaactc cattggcgct cggccacgaa ggcgccggta tcgtatgtgg tctaggtgac
     1561 ggggtcgaga gcgttcacgt cggcgatcat gtagttttgt gttgggcacc accatgcggt
     1621 gtgtgtgttt cctgcaaact tggccgtccc gtgctttgcg accggctgga gagaactacc
     1681 tttcggaatc gtttgccttc gggtggaact cgcctgcgcg ccaggaatca agacatcctt
     1741 catttcaaca gcactgcttg tttcgcagac ttcgctgttg tcagcgagca gggcatcgta
     1801 aaagtgcctc cacatattcc tttctcatca ctcgcgacgc tgggatgtgc agtaatcacc
     1861 ggtatgggag cagtcaccaa tgtggctaag ctttccggcc gagagagaat tgtagttgtt
     1921 ggagcaggtg gtgttgggct taatgtggtc cagggggcca agatagccgg ctgcgacgag
     1981 attatcgcag tcgacgcgaa gcccaaccct ctccacctgg ctcttaagtt cggcgcgact
     2041 cacactatcg actcttcgac gcaagatgta agcgacatag tcctgcaact cacggaaggc
     2101 cgtggcgcag actttgtgtt tgatacagtt ggcgcgccag caacagtcga gcaatcgttt
     2161 aaccttgctc gtaaaggtgg cagcgtcgtc gtaactggcc tggcacgcac tgattccctg
     2221 gcctcaattc cgatctttcc tctcgtaatg caggagaaac gatttattgg atccgtctac
     2281 ggatctggag atccgttgaa agacataaat aagctggtca agttgtatga acaaggtgta
     2341 ttaaaactcg aagagttagt ggcacgtgat taccgtctgg tccaggtcaa cgaagcgctg
     2401 tccgcactcg ccgctggaga aggagcccga gggatcattc tttggtgagc aattagtatt
     2461 tcaattgagg tgaagctcat gagaataatt gcaaatttgc tgcttgtctc tatctgctcg
     2521 gcaatcgctc tggctgctga cgactggtca caattccgtg gcccgaacgg caccggagtt
     2581 tctgcgacta ctggactccc tgtcgagttt ggaccatcta agaacgttct ctggaagact
     2641 gaactgccgc cgggacactc ttcgccggta ctcgcgggca agaaactttt cgtcactgcc
     2701 cacaccaaag agaaagagac ttacaagctg ctcgtatttt gccttgatcg ccagacggga
     2761 aaaattctct ggcaacgtga agtcgcacgc gcgcagaaag gacgcctcga gaatgtaaat
     2821 agtcctgctt ctccctctcc cgtaactgac ggcacgaatg tctacgtttt ttttcaggag
     2881 ttcgggttgg tctcatttga cggtgcgggc aaggagcgtt ggaaactgcc ccttggtcca
     2941 ttcaacatgt tctatggttt cggagcttcg ccaatccttg ttgacgacaa agtcattcta
     3001 cccgtcgatc aggacaatcc agcgtcatac ttgctcgcca tcgataagaa cagcggaaaa
     3061 gttcgctgga aggtcgatcg gcccggcgtg atttcgggtt actccacgcc gataatctac
     3121 caacctaaag atggaccaaa acagatcgtc gtccctgaat cgttccagct ttcagcttac
     3181 tcagttgttg acggcaaaag agtctggtgg gtccgcggcc tcgcttgcga gatgaagtca
     3241 attgcaagtc aggacggtga ttatctctac atcaatggct ggggatttcc tacgaaccaa
     3301 ccaggcgaac aagtcaagac cgtgccattc gacgcagcgc tcactagata cgataagaac
     3361 ggcgacagaa tgatcgagaa ggctgaggct accggcggtg agtcgatgga caagtggttg
     3421 aaccttggat tcgaagcatt cgacatgaac cgggatcaga aacttgatag ccccgattgg
     3481 gagatcttcc gttcaatgat ggcatcggaa aacggtctgc tggcgattaa gatgggaggc
     3541 acaggcgatc aaaccgcgtc ggcaattcgc tggcgctatc aaaagccagt acctcaggtg
     3601 ccctcaacgc tgctttacaa aggcgttctt tacatgatca acgacagtgg aattttgatc
     3661 tcatttgatc cggcaacggg aaatgtgatc aagcaaggac ggcttcgcgg cgcaattgac
     3721 aagtatttct catctccggt cgcagcggac gacaaagtct tcttaattgg cgagggtggc
     3781 gcagtctcag tgttgaaagc cgcgggggat tggcaagtcc ttgcggtcaa tgagctcgat
     3841 gacgagtgtt tcgcgacgcc cgcaatagcg gatggccgaa tctatataag aacccgcagc
     3901 gcactctatt gcttcggggc ttaatcttcg ttacacgcag ctgcctgttg ggtgaacccg
     3961 tggcgactgg tccaggaatt aagcgaggag gagttaaatt gtctgcagac ttctcatttc
     4021 ggtggagacc gaggctcgcc gcttctggtt ctttcgaatt ctcaccgggc ttcagcccgg
     4081 tgccaaatga cgtaccctta cacccgaacc gttttaacgg tttatcgatc ggcctagagc
     4141 gatgtgaaac cgttgaaacg gttccgctac caaccaatac cgtggacacc gggctgaagc
     4201 ccggtgagaa tgagagagac gcatcgcctt cacagactca aataaaaatg atcaatggcc
     4261 aatgatcaat gaccaatgga aggctctccc ctcagccacc agtaaaagct actccgatcg
     4321 cagcgccatt agcggatcaa tcttcatcgc gcggcgcgcc ggcagatagc acgcaaccag
     4381 cgacaccgtt acgagcagaa tcgaaatcgt agcaaacgtc gaaggatctg ctgccgcaac
     4441 tccaaacaac agtcccgaca agagtcgggt taacgcgaat gcggccacca atccgatagc
     4501 gactccaacc accgtaagca gcaagccctg tcttaagatt aacaacagca cctggcgagg
     4561 ctgtgctcca attgcgactc gtaatcctat ttcgtgcgta cgcaacgcta cggaataatt
     4621 catcacgcca aagatcccga ccgccgaaag taaggttgcc aggcccgcaa ataaggccag
     4681 gagtagcgtg ttgaaccttg cgcggctgaa cgtgtccgac atgacctggt ccattgtccg
     4741 cacatccgag acaggctgat ttggatcgag gcttctaatc tcgcgctgta gcgctggcgc
     4801 aatcgcgacc ggatcaccat cggtgcggat tacaaatgtc ataaatgaat aggccaattc
     4861 aggatgcgga aaatagactg ccggttcaaa cccatcaatg agactttcat acctcacatt
     4921 cccaacaact ccaatgatct cggtcgggtt tggaccatca aacatcgaga catctatcct
     4981 cttgccaata ggatcctcat cagcaaaata cttccgcgcg aacgcttcgt tgatcagcac
     5041 cacatgtttt acttctctat tttcgctctc gctaaattgc ctgcc
//
LOCUS       DASSFP010000305         6007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_384967, whole genome shotgun sequence.
ACCESSION   DASSFP010000305 DASSFP010000000
VERSION     DASSFP010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384967"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1284
                     /locus_tag="VFH91_06795"
     CDS             379..1284
                     /locus_tag="VFH91_06795"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET6862735.1"
                     /translation="MSAPANKQETWPALPLEEWEGTRATLHMWTQIVGKVRLAQTPLV
                     NHWWNVPLYVSARGLTTTAIAYQQTLFEMEFDFIDHNLVIKCSDGATVTVPLRPKSVA
                     TFYSETMAALGELGFDIHIWKMPVEVPNPIPFDQDQQHASYDPAYANRFWRILSAADK
                     VMQEFRSRFIGKCSPVHFFWGSFDLAVTRFSGRPAPAKPDADRITREAYSHEVISHGW
                     WPGQGPLGKPAFYSYTAPAPEGLSSAKLRPEAAFYSNELSEYLLLYDDVRTSANPDND
                     LMEFLQSTYEAGATLANWDRKALER"
     gene            complement(1354..2994)
                     /locus_tag="VFH91_06800"
     CDS             complement(1354..2994)
                     /locus_tag="VFH91_06800"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6862736.1"
                     /translation="MFQSQAIPAPTPWPTPWGPPPQAIFEKYFHDLVNKTWGNISPLY
                     KLDAFDWIILIAYFAILSTLAVYGAYRIKQVIDFWRYRKFVPRPVARFSPEDLPLITV
                     QLPLFNELYVVERLLKAVTAIDYPREKLEIQVLDDSTDETVKLAEAVVARYAEQGFDI
                     HYIHRSDRTGFKAGALENGNKTAKGELLAIFDADFVPKPDCLRKLVDFFTDPLVGCAQ
                     MRWAHINGNYNLLTKLQTIMLDGHFVVEQTTRNRTGGFFNFNGTAGIWRRKAIEMSGG
                     WQHDTLTEDTDLSFRAQLMGWKFVYLLDEESPAEIPVEINAFKAQQRRWAKGVMQVGL
                     KLYPRIWLAPLPLRVKLEMFFRLTGNISYPLMIIASFLQFPLLLVRYNQPFYHLMVLD
                     LPLLFFSSISVVLFYGTAVWYLDDKRTPRLLHLPLVMALGIGLAFSNARAVLEALLGV
                     KTEFVRTPKYRVEDTADETWKRKKYKRKRGLLPLLELGFALYFLLAMTYAVRLHMWGT
                     IPFLFLFFFGFAYMGVMSVMQTASGRRISTLWRPLKAR"
     gene            complement(3182..3766)
                     /locus_tag="VFH91_06805"
     CDS             complement(3182..3766)
                     /locus_tag="VFH91_06805"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HET6862737.1"
                     /translation="MEYRPQFAGIRAAVIAWALLSVATLVFVSFIPAAPLAAANNHPQ
                     LALAIYQPFHFLCHQLPERSFYLAGYKLAVCARCTGIYSGFLLLLLLYPLVRSLRETS
                     LPHRRWLLLATTPLVIDFGLTFLGIWENTHTSRFLTGFLFGAVTVFYIMPGILELSFQ
                     LNRTKSTKRQEFTVLSGESIAAAPSDYSAPARRI"
     gene            complement(3785..4834)
                     /gene="glpX"
                     /locus_tag="VFH91_06810"
     CDS             complement(3785..4834)
                     /gene="glpX"
                     /locus_tag="VFH91_06810"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET6862738.1"
                     /translation="MGHELRTERELSLDFLRVCEAAAIAAARTMGQGDRKYSDHVAVE
                     AMREVMDTVPMRGRVVIGEGERDEAPMLYIGEEIGGGFLLGDEAGPVCPEVDIAVDPL
                     EGTNLCALGANNAIAVLAAAERGGLLNAPDIYMDKIVVGPSSRGVVDIDAPVKDNLKN
                     IARRLGRDIGDLTVIVLDRSRHKKLINDVREAGARIRLISDGDLSAGISAAVAGTNIH
                     ALMGIGGAPEGVITAAAMKCLNGEIQARLVFDPERLGVDKAKVPARADVLDRLASMGI
                     RDPDKIYDTDDLAPGKKIIFAATGVTDGALLKGVRFFGSGKRTHSVVMTTESRHIRFV
                     DTLHIEGGPDTVIRF"
     gene            5115..>6007
                     /locus_tag="VFH91_06815"
     CDS             5115..>6007
                     /locus_tag="VFH91_06815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6862739.1"
                     /translation="MSTAANSRQINEDKMHEFLGKVISDFGASLSTALSYIGQKLGLY
                     KALASDGPLTAPQLAEKTKTNERYVREWLINQAAGGYVEYDPASGKYGLSPEQAVALT
                     DETSPFYVGGGFFVTKAMTQAVERIEDHFRNGGGMLWGEHHPDLFIGTERFFRPGYSA
                     HLIASWIPSLTGVEEKLKQGAKVADIGCGHGASTIIMAKAYPKSEFWAFDNHEASINH
                     AQKSAQDAGVANQIHFEITNAQSIPDKQFDLVTFFDCLHDMGDPVGACKRASEVLAPD
                     GTVMIVEPMAGNTVEENFNPIG"
BASE COUNT         1580 a         1555 c         1478 g         1394 t
ORIGIN      
        1 ggcgcgaccg gtatgacctt aataattgcg agtagttgtt agctcatctg cttcctccca
       61 ctgcgcacgg cccatcggtc gcgctgcaat aacagcacca tgagcagtaa gtacaacaga
      121 agcagtggtt catcattagg aagcaacccg tagagttgat gaaaaaacta tagggttggc
      181 caattctcct gtcaaggcta acgccgcttt gatttgtttg taggggcggc cctcccaaga
      241 aaccgcctgc tgtttcgggt ggtttcttgg gagggccgcc tttcaaattt agacacggca
      301 aaattgccgg cgacggtttg cgatctggaa gaacccaatt atagttgtca caccgttaca
      361 gcaagaaagg tagacctaat gtcagcacca gccaataaac aggaaacctg gcccgcactt
      421 ccacttgagg aatgggaagg cacccgcgcg acgctacata tgtggactca aatagtcggc
      481 aaggttcgcc ttgcccaaac tccacttgtt aatcactggt ggaacgtacc gctgtatgtt
      541 tcagcacgtg gtctaacaac gaccgccatc gcttatcagc agacgctctt cgagatggaa
      601 ttcgatttca tcgatcacaa tctggtaata aagtgcagcg atggagcgac ggtgacggta
      661 cctttaagac cgaagtcagt cgctactttt tacagcgaaa caatggctgc gttaggtgag
      721 cttggatttg atattcacat ctggaaaatg ccggttgagg tgcccaatcc gattcccttt
      781 gatcaagatc agcaacacgc ttcctacgac ccggcgtatg caaatcgatt ctggcgtatc
      841 ttgagcgcgg ctgacaaagt gatgcaggag tttcgctctc gattcatcgg caagtgcagc
      901 ccggtccatt ttttctgggg cagttttgat ctcgctgtca cgcgcttttc aggacggccc
      961 gcacctgcga aacccgatgc cgatcggatt actcgcgaag cctactcgca cgaagtcatc
     1021 agtcacggct ggtggcccgg acaaggaccg ttaggaaaac cagcgttcta ttcttacact
     1081 gcgccggcgc ccgagggcct tagcagcgcg aagctcagac ccgaggcggc tttctacagt
     1141 aacgaactgt cagagtattt gttgctttac gatgatgtgc ggacttcggc gaatcccgac
     1201 aacgatctaa tggaattttt gcagagtact tacgaggctg gtgctacgtt agctaactgg
     1261 gatcgaaagg cgcttgaaag gtaagcgctt gcgctttgta ctttgtacct ggtaatggaa
     1321 tacaaaatac aaagtgcaaa gcaccatctc caactacctc gccttaagtg gtcgccacaa
     1381 tgtactaatc cttcgaccac ttgccgtctg cataacgctc atcactccca tgtaggcgaa
     1441 tccaaagaag aaaagaaata agaacgggat tgtaccccac atgtgcaatc gaactgcgta
     1501 cgtcatcgcc aacagaaaat agagcgcgaa tcccaattcc aacagcggca acaaccctcg
     1561 cttgcgcttg tatttctttc gtttccaggt ttcatccgcc gtgtcctcaa ctcgatactt
     1621 cggagttcgc acaaactcag tcttcacgcc cagcaatgct tccaatacgg cacgagcgtt
     1681 tgaaaacgcc aggccaatac caagagccat taccagaggc agatgtaaca aacgcggtgt
     1741 tcttttgtcg tcgagatacc aaacggccgt tccataaaac agcacgacgg aaatggacga
     1801 gaaaaacaac aaaggtaaat ccaagaccat caagtgataa aacggctggt tataacgaac
     1861 caatagcaac gggaactgca aaaagctggc gataatcatc agcggataac tgatgtttcc
     1921 ggtcagacgg aagaacatct ccaacttcac gcgtagcggc aacggagcca gccagattcg
     1981 gggatacaac tttaatccaa cctgcatcac gcctttggcc cagcggcgct gttgagcttt
     2041 gaacgcattt atctcaactg gtatttcggc cggcgattct tcgtcgagca ggtagacgaa
     2101 tttccatccc atcagctgtg cacggaaact gagatcggtg tcttcggtca aagtatcgtg
     2161 ctgccagccg ccgctcattt ctatcgcctt gcgacgccag atcccggctg ttccgttaaa
     2221 gttgaaaaag ccgccggtgc gatttcgagt tgtttgttca accacgaaat ggccatcaag
     2281 catgatggtc tgaagtttgg tgagaaggtt gtaattgcca ttgatgtgtg cccaacgcat
     2341 ctgcgcacaa ccaacaagcg gatcagtaaa gaaatctacc agcttccgta aacagtcagg
     2401 cttcggaaca aaatcagcat caaaaatcgc aaggagttcg cccttggcag tcttgttccc
     2461 attctccaaa gcaccagcct taaaaccggt ccgatcggaa cgatggatgt agtgaatatc
     2521 gaatccctgc tcagcgtacc gggcaacgac tgcttcagct aacttaacgg tttcgtctgt
     2581 tgagtcgtct aagacctgta tctctaattt ctcccgcgga taatcgatag cagtgaccgc
     2641 cttgagcaaa cgctcgacaa cgtacagctc gttgaacaac ggtaactgga cggttatgag
     2701 gggcagatct tcgggactga atcgagcgac cggcctggga acaaattttc gataacgcca
     2761 aaaatcgatc acttgcttga tgcggtaagc gccgtagaca gccagagtgc tcagaatagc
     2821 gaagtaggcg ataagaatga tccagtcgaa tgcatccagc ttgtaaagtg ggctgatatt
     2881 gccccaggtc ttatttacga gatcgtgaaa atacttctcg aaaattgctt gcggtggagg
     2941 cccccacggc gtcggccacg gggttggagc tggaattgct tgactttgaa acatcatcat
     3001 ttcggtgtaa cgagcgcctt acgtttaaaa cgccgtcagc taaaatgccg gacggcgcga
     3061 tagaagtgcg ttagatgcgg ttttttaagc tatcgaacca caatttttgg cggagtgatt
     3121 ctacccgatc cgccgcacta ttcagaagcc gcgggcctat ctttgaactc caggatcgaa
     3181 ctcagattcg ccgggccggg gcgctgtagt cactcggagc cgcggcaatg ctctcaccgg
     3241 aaagcaccgt aaattcctgc ctttttgtcg atttggtgcg attcagctga aagctcaact
     3301 cgagaatacc cggcatgatg tagaagacag ttacggcacc aaacaaaaat cccgttagaa
     3361 atcgtgaggt gtgagtattt tcccagatac caagaaaagt cagtccaaaa tcaattacca
     3421 acggagtcgt agcaagcaaa agccaccgac gatgaggcaa acttgtctcg cgaagagaac
     3481 gaaccagcgg atacaacaat agcaacaaca aaaagcccga gtatattcct gtacagcggg
     3541 cacacacggc taatttataa ccggcaaggt aaaacgaccg ctcaggcaac tgatggcaga
     3601 gaaaatggaa tggctgatag atcgcgaggg ccagttgagg gtggttgttt gccgccgcga
     3661 gtggtgcagc cggaataaat gagacgaaaa ccagcgtagc gactgagagt aaagcccagg
     3721 cgattacagc tgctctgatt ccggcaaact gtgggcgata ctccatacgc gatctagaac
     3781 tactttagaa tctgatgaca gtgtctgggc caccctcaat atgcagggta tcaacaaatc
     3841 gaatatggcg cgattcagtt gtcattacta ctgaatgggt acgttttccc gagccaaaaa
     3901 atcttacgcc tttcaaaaga gcaccgtcag ttacacctgt cgcagcgaag ataatctttt
     3961 tgcccggcgc cagatcatcc gtgtcgtaga tcttgtctgg atctcgaatt cccatcgatg
     4021 ccagacgatc taaaacgtca gcgcgcgctg gcactttagc tttatcgacg cccagccgtt
     4081 ccggatcaaa aaccagacgc gcctgaattt cgccattcaa gcacttcata gctgcagctg
     4141 tgatcacgcc ttctggagcc ccacctattc ccatcaaagc gtgaatgttg gtgcccgcca
     4201 cggccgctga gatgcctgca gacaggtcac cgtcggagat cagacgtatt cgcgcgccag
     4261 cctctcgcac atcgttaata agcttcttat gacgtgagcg atcgaggaca atgacagtca
     4321 agtctccgat atcacgccca agtcgccgcg caatattctt tagattgtct ttcaccggag
     4381 cgtcgatatc cactacacct cgtgatgatg gaccaacgac gattttatcc atgtaaatgt
     4441 caggagcgtt caagaggccg ccacgttccg cagcagccag cacagcaatg gcgttgtttg
     4501 ctcctaacgc acaaagattt gttccttcca gtggatcaac agcgatgtct acttccggac
     4561 aaactggtcc ggcctcgtcg ccgaggagaa atccaccacc aatctcttcg ccgatgtata
     4621 acatcggggc ttcgtcgcgt tcaccttcgc cgatgacaac tcgaccgcgc attggcaccg
     4681 tatccatcac ttcgcgcatc gcttccactg ctacgtgatc ggagtacttg cgatcgccct
     4741 ggcccatcgt tcgcgcagcc gcaatggcag ctgcctcaca aacgcgtaag aaatcgagag
     4801 acaactcgcg ctctgtcctc aattcgtgac ccatgctttc agaatctcct gtctgtggtg
     4861 ttttggcttg ttcagcaccg gggcagcttc agcctcagtt ttcgagaatt gcaggccaat
     4921 actaacagag agaactacaa ttttgtaggc tgcaatcagg gctcacgata aaggtggcac
     4981 accaagcgcg gcaagtaaat tgaccgtaga gttgtggatc tagagcatga tcattgcttg
     5041 acttgcccgg gtacgacgag taagagatac ccgtcgaaat ccccctttgt cgaataataa
     5101 ttctgggagg ctttatgtct acagctgcaa attctagaca gatcaacgaa gacaaaatgc
     5161 acgaatttct gggtaaggtc atcagcgatt ttggcgcatc cctgagcaca gcgctctcct
     5221 acatcggaca aaaactcggc ctttataaag ccctggcgag cgatggcccc ctgactgcgc
     5281 cccaactcgc ggaaaaaacc aaaactaacg aacgctatgt ccgggagtgg ctcatcaatc
     5341 aggctgccgg gggatatgtc gaatacgatc cagcaagcgg caaatatgga ttatctcccg
     5401 agcaagctgt cgcgcttact gatgaaacga gcccttttta cgttggtgga ggcttctttg
     5461 taaccaaggc catgactcaa gcggttgaac gaattgagga tcactttcga aatggcggag
     5521 gaatgttatg gggcgaacat catcctgatt tatttatcgg gactgagcga ttctttcgtc
     5581 ccggttactc tgctcatctg attgccagtt ggattccctc tttgaccgga gttgaggaaa
     5641 aattgaaaca gggagcgaaa gtggctgaca tcggatgcgg gcacggagct tcaaccatca
     5701 ttatggctaa agcttatccg aagtcggagt tttgggcttt cgacaatcat gaagcgtcga
     5761 ttaatcacgc ccaaaagtca gcacaagatg ctggtgttgc aaatcagatc cactttgaga
     5821 taacgaatgc tcaatcaatc cctgataaac agtttgacct tgtcaccttc tttgattgtc
     5881 tgcacgacat gggcgatcca gtcggtgcgt gtaaaagggc ttctgaggta ttagctcctg
     5941 atggcaccgt catgattgtc gaaccgatgg caggcaatac cgttgaagag aatttcaatc
     6001 caattgg
//
LOCUS       DASSFP010000306         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_385589, whole genome shotgun sequence.
ACCESSION   DASSFP010000306 DASSFP010000000
VERSION     DASSFP010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385589"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..739
                     /locus_tag="VFH91_06820"
     CDS             317..739
                     /locus_tag="VFH91_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6862740.1"
                     /translation="MKSANKSNKSLVNTQELGRAVKRKREQLSLSLRDVADVTGVSAS
                     TLSRIENGTGKPDADNIARLTSWLDVPMERILAGRNPETTESKPVVYYPQEPMPEIIE
                     AHLRADKNLTPDTAAALSELFRVAYTQFARNEDTRKKR"
     gene            896..1474
                     /locus_tag="VFH91_06825"
     CDS             896..1474
                     /locus_tag="VFH91_06825"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HET6862741.1"
                     /translation="MRIDLPLNPFELARFANLVVIKIEQLESLSEAAREHLLGPAAEE
                     WSGGACSLPDGSKLVILNPTHGPARTNATLMEEICHVFLGHEPNKLSIVTKDDRGKVR
                     SREYRQNDEEEAYGVGAACLVPYTSLKNLLHLGKSSKQIAAHFRVSRELVEYRMKVTR
                     LWREFKQLGGLGFWPLAFGGIASIFRAFKFHG"
     gene            1618..2919
                     /locus_tag="VFH91_06830"
     CDS             1618..2919
                     /locus_tag="VFH91_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HET6862742.1"
                     /translation="MTEQLNELKAQGLTTVDKLRMLLDITNKISRTLDLQEVLNLVMD
                     TLDSLIPYDAAGIFVLQCLDKDLVPEGEEPCTFKSEAVRGYDIAELSDLHLKLGEGFI
                     GNVAVSGEPVISHDVRHDPVYINARDRTRSEMVAPIISNDEVIGVFDLESDELNAYSD
                     DDLEVLMLLASQVAIIIEKVMLHEQLIEKKRLEGQLEVARQVQLELLPARDPDLPGYD
                     ISAYNYPTEEVSGDYYDWVPIYEDQIGIVIADVAGKGVPAALLMAFLRASLRSASHTG
                     YATHISMAKVNYLLWESIERNQYVTAFYGILDASNKTLSYSNAGHNPPLLLTADGSRT
                     QFIRRGEQPLGMFRDTRYHEYYLSFDPGDLLVLYTDGATEALNPAGEEFGRDRLAEAV
                     KASYNLTARELVSSLERAVLDWTEGAGATDDITFFVIKALK"
     gene            complement(2974..3231)
                     /locus_tag="VFH91_06835"
     CDS             complement(2974..3231)
                     /locus_tag="VFH91_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862743.1"
                     /translation="MATYRDPVSCFAPLEVIERLLEIRVEAGFSPAVYLLSPDDLADY
                     LDAYGFDVYALSEAEAEFHFGDYRAPLAPGGDHAHCSLTAH"
     gene            3394..4599
                     /locus_tag="VFH91_06840"
     CDS             3394..4599
                     /locus_tag="VFH91_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET6862744.1"
                     /translation="MSTEQPTNPASPPPPNFLNVLREMLRASDKVSDLIFSPGRPPQI
                     EIAGKLVPVPIAGLEKLTAGHTAGIAKLIIGNHQIAAESLEKTGSADLSFSAPGDARF
                     RVNIFKQRGTHAIVMRVIPMRPPRFEDFNLPEQLKEIGEIKNGIVLVTGPTGSGKSST
                     LAAVIDLINETHYYHIVTIEDPIEFLHNHKNSTIHQRELHSDTPSFSVALRAALRQAP
                     KVILVGEMRDRETIEVALEAAETGHLVLSTLHTIDASKTVDRIIGVFPKNEEKAIRTR
                     IAQSFRFIISQRLIPVADGKGRIAAIEILKSTMRTREYIEKGDAEGKSLIDAMQQGDQ
                     DGMQTFDGVIEKLIRSGVVTIEGAIPYATNAHNLLLQISDLDGSPAPKPAETKPQTKD
                     DSMLDMIER"
     gene            4661..>5560
                     /locus_tag="VFH91_06845"
     CDS             4661..>5560
                     /locus_tag="VFH91_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862745.1"
                     /translation="MLTPISSRSIQTDSDIRIDVPPDGIVKIDNRFGNVSAEVWSNRF
                     VSVSANVEGNARLSRSPILLDNRNSVLSISVVRSPLDPAVAVHLIVKIPDTSRLEIGT
                     NTGSITLNGLSAKASLKSVSGDIQSKLPDGIDAKIKAQTLRGVVTSVIPNSPVTDNHL
                     FQVNLGTGVRMLDIQSQSGLISLVSSGVTAETKPAEMKAPALIGPEAATRTAGVPDKQ
                     PETDEISEGDVIRVDSQLVTINMSVTDRNTNRGFSDLRQSDFTLFEDGQQQQIVQFDS
                     ASAPFDMILLIDLSGSTKDVVKLI"
BASE COUNT         1458 a         1347 c         1425 g         1330 t
ORIGIN      
        1 aactcgaact gcgattcaat ctcgtcacgg cagcttatgg cagaattatg ggcgacaggg
       61 ttttcataac gactgaggat ccgcaggttg taaaggccgt agatgtgttg cccaaggccc
      121 gtgacctggc gatgagtagt acttcatctc gagctgagaa gtagtaccag gatttgggcg
      181 taacataacg agggccgcgt gaagtcgcgg ccctttttct tgtgtccagg taattccgtg
      241 tttgattgag gagttttgtt tcagggataa gacaaagtct tgcgtctaaa accagggatg
      301 tgttagggtt gtggtcgtga aatctgcaaa caagtcgaat aaatcactgg tgaacacaca
      361 ggagctgggg cgcgccgtga aacgaaagcg tgaacagctc agtttaagtt tgcgagatgt
      421 tgccgatgtc accggtgttt ccgcatctac gctttcacga atcgagaacg ggactggtaa
      481 gcccgacgcg gataacatcg ccagactgac ttcatggctt gacgtgccaa tggaacggat
      541 acttgctggt cgcaatcctg agacaaccga atccaagcca gtcgtttatt atcctcaaga
      601 accaatgcct gagattatcg aagctcacct tagagccgat aagaacctca ctcccgatac
      661 tgctgctgcg ctttcggaat tattccgggt agcctatacg cagtttgctc gcaacgaaga
      721 caccaggaaa aagcgttaag gcagttgtaa gttttgcgct tctgaatgcg gacccgacgg
      781 ccgcagatca gccaagatcc cttatactgt tagagtgttg gtacgcgatc taccccccgg
      841 agaaaaatgg cgcaaattcg aactcaaagc attaggtctg cgagatttcg cgcaagtgag
      901 aattgacctg cctctcaatc cctttgagct cgcacgtttc gctaacctgg ttgtgatcaa
      961 gattgaacaa ctcgagagtt tgagcgaggc agctcgcgaa catttgcttg ggccagccgc
     1021 cgaagaatgg tcgggcggcg cctgctcgtt acccgatgga agtaaacttg ttattctcaa
     1081 tcctacgcac ggcccggccc ggaccaatgc gaccttgatg gaagagatat gtcatgtttt
     1141 tctaggtcac gagccgaaca agttatccat tgtgactaaa gatgatcgtg gcaaagtgag
     1201 atctcgcgag tatcggcaga atgacgaaga agaggcttac ggcgttggcg ctgcgtgttt
     1261 ggttccttat acatcgttaa aaaatctatt gcatctgggg aagagttcaa agcagattgc
     1321 tgcccacttc cgtgtaagtc gcgagttggt tgaatatcgg atgaaggtga cgagattgtg
     1381 gagggagttt aagcagctgg gtggtttggg gttttggcct ttggcctttg gcgggatcgc
     1441 tagcattttt cgggcattca aatttcacgg ataagaaaca aaatgcccaa gttccaaagg
     1501 ccaaagaccg aaggccgaag accaaaggcc accccgaagg ggtagacaga ccaacgggca
     1561 aaaaacctct ataatcccca ttcgactacg tggcagaccg agtcgagaaa ctttaggatg
     1621 acagaacaac taaacgaatt aaaagcgcag gggctaacga cggtcgacaa gctgcgcatg
     1681 ttacttgaca tcacaaacaa gattagtcga acgctcgatc tccaggaggt cttgaacctt
     1741 gtgatggata cgctcgactc gctaatccct tacgacgccg caggcatttt tgtcttgcag
     1801 tgccttgaca aggacctcgt accagagggc gaggagccat gcacatttaa gtctgaagca
     1861 gttcgcggct atgacatcgc cgagctatca gatctgcatt tgaaactggg cgagggtttc
     1921 atcggcaacg tagcagtcag tggcgaacca gtaatctctc acgacgttcg ccatgatccg
     1981 gtttatataa acgcccgaga ccgtacacgc tccgaaatgg tcgcgccaat catttccaac
     2041 gacgaagtga tcggagtatt cgatcttgag agcgatgaac tgaacgctta ctccgacgat
     2101 gatctggaag tactgatgtt gcttgcatcg caagtcgcaa tcatcatcga gaaggtaatg
     2161 ttgcacgaac aactgatcga gaagaaacgt ctcgagggtc aactcgaagt agctcgtcag
     2221 gttcagttgg aactattgcc tgcgcgagat ccggatctgc ctggctatga catcagtgct
     2281 tataactacc ccactgaaga agtttccggc gattactacg actgggtacc gatttatgaa
     2341 gatcaaatcg gcatcgtgat tgccgacgtc gcggggaagg gagttccggc tgcgctactg
     2401 atggcattct tgcgtgcgag cttgagatcg gcgagtcaca ctggttacgc aacccacatc
     2461 tcgatggcaa aggttaatta cttactgtgg gaatcgatcg agcgaaacca gtacgttacg
     2521 gctttctatg gaatactcga cgcctcaaat aaaactcttt cgtattcaaa tgccggtcat
     2581 aatccgccgc ttctgcttac cgcagatggt agtaggactc agttcatccg tcgaggtgag
     2641 cagccgttgg gcatgttccg cgacacgaga tatcacgaat actatctttc attcgatcca
     2701 ggagacctgc tggtcctcta cactgacgga gcgactgaag ctttgaatcc ggcgggagag
     2761 gagtttggac gtgaccgact ggctgaggct gttaaggcct catataacct gactgcgcgc
     2821 gagttggtgt catcgctgga gagggcggtt ctcgactgga ccgagggcgc gggcgcaact
     2881 gacgacatta ctttctttgt aataaaggcc cttaaatagc aaaacgcggg aactactatc
     2941 gtcccgcgtt gctttacgaa ttgatttgtg attttaatga gccgtcagtg aacagtgcgc
     3001 gtgatcgcca ccgggtgcga gtggagcgcg atagtcccca aagtgaaact cggcttcggc
     3061 ctccgataat gcatacacat cgaagccata cgcatcgaga tagtctgcca aatcatcggg
     3121 ggaaagaaga tatacggcag gcgaaaaacc tgcttcaaca cgaatttcca gcaggcgctc
     3181 gattacttca agtggtgcaa agcagctaac aggatctctg tacgttgcca tgttaagtaa
     3241 gcgccctttc tttcaaaatt cgggactcgg tccattggtc cgaccgcgtc ctttccttaa
     3301 tcacttgctt tgatgtacgc aaggaccaag tagtttgttc gcttcctaca tttcttttga
     3361 cgcccgatcc tattgaaaag ttccttttat aaaatgagca ccgaacaacc aactaatccc
     3421 gcttcgccgc caccaccaaa ctttctcaac gttctgcggg aaatgttgcg cgcgtccgac
     3481 aaagtcagcg atttaatttt cagccccggt cgaccaccac aaattgagat tgccggaaaa
     3541 ttggtgccag tccccatagc tggtctcgag aagttgactg ccggtcatac ggccggcatc
     3601 gctaaactaa ttatcggaaa tcaccaaatt gctgcagaga gtttggagaa aacaggctcg
     3661 gcggatctat cgttcagcgc accaggcgac gctcgttttc gagtgaatat ttttaaacag
     3721 cgcggcacac atgcgatcgt gatgcgagta attccaatgc gccctcctcg cttcgaggac
     3781 ttcaatcttc ctgagcaact aaaggaaatt ggggaaatta agaatggaat tgtgcttgtg
     3841 acaggtccaa ccgggagcgg taagagctct accctcgcag cagttatcga tctgataaac
     3901 gagacgcact attaccatat cgtcaccatt gaagacccca tcgagttcct ccacaaccat
     3961 aaaaactcga ccattcatca acgagaactc cattcggata ctcccagttt ttcagttgcc
     4021 ttgcgagcgg cactcagaca ggctccaaag gtcattctcg ttggtgaaat gcgcgaccga
     4081 gaaacaattg aagtggctct ggaagcggct gaaacgggtc acctcgtgct ctccacgttg
     4141 cataccatcg atgcttcgaa aacagtcgac cgcataatcg gggtgtttcc gaaaaatgag
     4201 gaaaaggcaa ttcgaacacg catcgcccag tcatttcgct tcatcatttc acagagactg
     4261 attccggttg cggatggcaa aggccgaatt gccgcaatcg aaattttgaa gtcgactatg
     4321 cgaacgcgag agtacattga aaaaggagac gcagaaggaa agtcgctaat cgacgcaatg
     4381 cagcagggcg atcaggatgg aatgcagact tttgatggcg tgatagagaa gctgattcga
     4441 tcgggcgtag ttaccattga aggtgcaatc ccatacgcga cgaacgcgca caacctgctg
     4501 ttgcagattt ccgatctgga cggttcgcct gctcccaaac ctgctgaaac aaagccccag
     4561 actaaggacg actcgatgct ggatatgatc gaacgctaat cctattatta tgttttggag
     4621 ctctaagagg cagattgtca gcttgttcct gttctttgga atgctgacgc caatttcaag
     4681 tcgaagtatt caaaccgaca gtgacatcag gattgatgtg ccacctgacg gaattgtcaa
     4741 aatcgacaac aggttcggaa atgtgtcagc tgaggtgtgg agcaatcgat tcgtctctgt
     4801 ttcggcgaac gtcgaaggaa atgctcgtct cagtcgctca ccaattctct tagacaatcg
     4861 aaactctgta ttgagcattt cggtagtcag atcgccgctg gacccggctg tcgccgttca
     4921 tttgattgtc aaaattccgg atacttcaag actcgagatt ggtaccaaca caggctctat
     4981 cacgctaaat ggactttccg cgaaggcaag tttgaagtct gtctctggtg atatccaatc
     5041 taaattgcca gacggcatcg acgcaaagat taaggcgcaa acacttcgtg gagttgttac
     5101 ctcggtcatt cccaattcac ctgtgacaga caatcatttg tttcaggtaa acctgggaac
     5161 tggggttaga atgttggaca ttcaaagtca aagcggtctg atatcgttgg tgtccagtgg
     5221 cgtaactgca gagactaaac cggctgagat gaaagcgccg gcgttaattg gaccagaggc
     5281 tgcaacccgc acggctgggg ttccggataa gcaacccgaa acggacgaaa tcagcgaagg
     5341 cgacgttatt cgcgttgatt cgcaactggt tacgatcaac atgagtgtta ctgatcgaaa
     5401 caccaaccgc ggtttctccg acttgcgtca atcagacttt acgttgtttg aagacggtca
     5461 acagcaacaa attgtgcaat tcgactcggc ctcagcgcca ttcgatatga ttctcctgat
     5521 cgacttgtca ggatctacaa aagacgtcgt caagctcatt
//
LOCUS       DASSFP010000307         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1572378, whole genome shotgun sequence.
ACCESSION   DASSFP010000307 DASSFP010000000
VERSION     DASSFP010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572378"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFH91_06850"
     CDS             complement(<1..717)
                     /locus_tag="VFH91_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862746.1"
                     /translation="MPGIRQDLQFGVRMLFKKPSFTAVVVLTLAMSISATTVVFSIVN
                     ALLLSSLPYRNPEQLINVWGVFKSNNKAHASAANFREWQERNKSFQSLAAYDMQKFNL
                     TGGDRPEAVDAAIISANLLPLLGVQPARGRSFQAEEEQSGNNHVAIISDGLWQRRFGA
                     DAGALGKPLLLDGESYTVIGIMPPGFSFPEKIDLWLPLSFVPEELADRGYNHLVVVGR
                     LKPGVDLRQAQAEMSALADEQ"
     gene            complement(875..>2498)
                     /locus_tag="VFH91_06855"
     CDS             complement(875..>2498)
                     /locus_tag="VFH91_06855"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET6862747.1"
                     /translation="RNYLIPVNADIQNEADYKYKTVDLNWALDQMYSEQNPLNIVILD
                     ACRSDPFPRSHRSDQGGLAQVSAPTGTLIAYATAPDSTASDGDGANSPYTQELIKQMR
                     VPGVLVETMLRHVAEQVSARTGGKQEPWFNANIKGDFYFNSGTGPTATTKKDTNPRPS
                     VSTPIDVPEAPRSKPEEDSAVARAREELRGRGIAIDVTSVKNALMSIDLDVLKLLTAA
                     KIQPSLIEEAFRQGSGDGTTVARKFFDNSSKSPEAIKWFDSALANGVDPNFTVPNDYY
                     DREGVLIEAVRAANVPAIKVLLERGASPHAYQNLFLTRYSLTRFLYPLRSIADDDRLT
                     LEEKQDLTKAFIKAGVVIPRVFDPGQSGWPSVMYEAKNLIDQDAPKLSINFSPSQPFC
                     NQPENSICKHAGSDWCSTIAKMPNKLVFDFKKNSSSPLYDVTLLHLLSIQGNKAYFLG
                     LTKYITYDYVLVEVSKDASSWTVLRYMAPEAGMGLCKKDSDGYQSEYCWRRIPIHRVA
                     TSDEMRFDDFGLSWRVSREECGSLYPKDASKK"
BASE COUNT          575 a          640 c          623 g          660 t
ORIGIN      
        1 ctgctcatcc gcaagcgcgc tcatctctgc ctgcgcctgg cgcaaatcaa ctcctggttt
       61 caaacgtccg acgacgacga ggtggttata acctcggtca gctaattcct ctggtacgaa
      121 actgagcggg agccagaggt ctatcttttc cggaaagctg aaacccggcg gcataattcc
      181 gattacggta taactttcgc catcaagcag gagcggtttc cctaaagcgc cggcgtctgc
      241 gccgaaacgc ctctgccata aaccatcgct gatgatggct acatggttat taccggattg
      301 ttcttcttca gcttgaaagc tgcgtcctcg cgccggctga acaccgagaa gcggtaacaa
      361 gttggccgag atgattgcgg catcaaccgc ttcgggacga tcgcctccag taagattaaa
      421 tttttgcatg tcgtatgcag cgaggctctg gaagctttta ttccgctctt gccattcgcg
      481 gaagttggcg gctgacgcgt gggctttgtt gttggattta aaaacgcccc atacattgat
      541 gagttgttca ggattcctgt aaggcaaaga actcaacagc aacgcgttga cgatgctgaa
      601 gactactgtg gttgcgctaa tgctcatcgc cagagtgagg accactacgg ccgtgaagct
      661 cggcttcttg aaaagcatcc gtacgccaaa ttgtaggtct tgtcgtatac ctggcatcgc
      721 attagcctcc tgattttttc aaagatatac agacagtact aattatgaga tcagatatcg
      781 gaggtcttgt cgatcaatcc gaggcggccc aaattcaact ggcgagacgc gactccgcgt
      841 ttgactctct ttcacttttt tcataggcga gaacctattt tttgctcgca tcttttgggt
      901 aaagagagcc acattcctcc cggctaactc gccacgagag gccaaagtca tcaaaacgca
      961 tttcatcgct ggttgctaca cgatgaattg gaatccgcct ccaacaatac tcggattgat
     1021 agccatcgct gtccttctta caaagtccca tgcctgcctc cggcgccata taacgcaata
     1081 cggtccaact gcttgcatct ttcgagacct cgaccagcac ataatcgtaa gtgatgtact
     1141 tggtcagtcc caggaagtag gctttatttc cctgaatact cagcagatgt aacagcgtta
     1201 catcgtacag aggtgaactt gaattcttct taaaatcaaa gactaacttg ttaggcattt
     1261 tcgcgatcgt tgaacaccaa tcactacctg cgtgcttaca gatcgaattc tcgggttggt
     1321 tacaaaatgg ttgggaaggc gaaaaattta tactcagctt cggtgcgtcc tggtctatga
     1381 ggttttttgc ttcatacatg actgagggcc agcccgattg acccggatca aagactctcg
     1441 gaataactac tcccgccttt atgaatgctt tagtgagatc ctgcttttcc tccaaagtca
     1501 ggcggtcgtc gtcagcgatt gatctcagtg gatagaggaa acgggtaagt gagtaacgag
     1561 tcaggaacaa gttctgataa gcatggggac ttgcaccgcg ttccagaagc accttaatgg
     1621 caggcacatt tgcggcgcgt acggcttcga ttaaaactcc ttctcggtcg taataatcat
     1681 tcggaactgt gaaattcggg tcgacgccgt ttgccagcgc tgaatcgaac catttgattg
     1741 cctcaggtga tttgctggaa ttgtcaaaaa acttgcgagc caccgtagta ccgtccccac
     1801 ttccttgccg gaaagcctct tctataagac tcggttggat ttttgctgcg gtcaaaagct
     1861 tcaatacgtc caaatcaatc gacatcaatg cgttctttac gctggtaaca tcgattgcta
     1921 ttccgcgtcc gcgcagctcc tctcgtgcgc gtgcgactgc ggaatcttct tctggcttgg
     1981 aacgtggagc ctctggaacg tcaatcggcg tggaaacgga aggtcgaggg tttgtatcct
     2041 tcttggtcgt tgcggttgga ccagtgccgc tgttgaagta gaaatctcct ttgatgtttg
     2101 cgttaaacca gggctcctgc ttgccaccag tgcgggccga tacttgttca gccacgtggc
     2161 ggagcatggt ctccaccaac acacctggaa cgcgcatctg tttgatcagc tcttgggtgt
     2221 atggactgtt cgcgccgtcg ccgtcactgg cagtgctgtc gggtgcagtc gcgtaagcaa
     2281 tcaaggtccc agtgggagcg ctcacctggg ccaggcctcc ctggtctgag cgatgacttc
     2341 ggggaaatgg atcgctcctg caggcatcca aaattacgat gttcagcgga ttctgctcgc
     2401 tatacatctg gtcgagcgcc caattgagat caacagtctt gtatttgtaa tctgcctcat
     2461 tctgaatgtc tgcgttaacc gggatgagat agttacgt
//
LOCUS       DASSFP010000308         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2024403, whole genome shotgun sequence.
ACCESSION   DASSFP010000308 DASSFP010000000
VERSION     DASSFP010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024403"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VFH91_06860"
     CDS             complement(<1..1282)
                     /locus_tag="VFH91_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET6862748.1"
                     /translation="MSWEFLQHNWPELLLLTRQHIQLVSASILAAVLIGVPTGILITR
                     YRKLHNPVLGLANIMQTIPSLALFGLLIPLPFIGGIGWRTALVALVLYSLLPIIRNTV
                     TGILGVDPNVREAAIAMGMTDRQMLWQVELPLAMGVIITGVRIATVIAVGVTTIAAAV
                     GAGGLGVYIFRGLRQYDNNLLLSGAIASAGLALVADFLLGLLEQRFSLERKDRGLALS
                     QKILLVVILLFAGLGTLASLRSQRAATTPTNAGQVVIASKDFTESILLAEIISQLLEA
                     RGVVVQRRFELGGNLPHEALLSGEADLYPEYTGTAFTAILHHQPITDPRAVYDQTKKE
                     YEDKLGLELSAPLGFENTFAILVRGNDARKLRLTAISDAVPYAPQWRAGLGQDFMSRA
                     DGYPGFSKAYGLKFAEVREMDLSLTYIALASNKVD"
     gene            complement(1279..2055)
                     /locus_tag="VFH91_06865"
     CDS             complement(1279..2055)
                     /locus_tag="VFH91_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6862749.1"
                     /translation="MTAPPDKIIVEFRDATFSLPTAAAPIIDHLNLTVSNGETLVLLG
                     ESGCGKTTTLKLINRLLSPTSGEVLVEGKSTMSWNAIELRRHIGYVIQEGGLFPHFTI
                     GRNVGLVLELLAWPAQKRTERARELLQLVGLEPNRFIDRYPRELSGGQRQRVGVARAL
                     AGDPSLLLLDEPFGALDPLTRASVQKEFAELARRVNKTAVLVTHDVREALLLGDRIAL
                     MDAGKIVLLETPAGFERSDNPKARAYIETLQMEKVSDQDQ"
     gene            complement(2064..>2481)
                     /locus_tag="VFH91_06870"
     CDS             complement(2064..>2481)
                     /locus_tag="VFH91_06870"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET6862750.1"
                     /translation="SGDALLLGADLKKDPAILEAAYNDALGVTSAFNLNVLARINREL
                     GGTFDLRAFKHYAFFNESSSRIEIYIESLRNQTVRIEKLDLEVEFSAGELIHTENSHK
                     YDLDSIAKLAEATGFKCARTWFDSQERFSSNLLLAS"
BASE COUNT          606 a          652 c          648 g          575 t
ORIGIN      
        1 aatctacctt attggaagca agcgcgatat atgtaagcga aagatccatt tcgcgcactt
       61 ctgcaaactt taatccgtac gcctttgaaa aacctggata accatccgcg cgcgacatga
      121 agtcctgtcc gagaccggca cgccattgag gcgcatacgg gaccgcatcc gagatcgcgg
      181 tcagccgtaa ctttcgcgcg tcgttacctc gcacaagaat ggcaaacgtg ttttcaaaac
      241 cgagcggtgc gcttaattcc aaaccgagct tgtcttcgta ttcctttttg gtttggtcgt
      301 aaacggctcg agggtcagta ataggctggt ggtgcagtat cgcagtgaag gcggtgccgg
      361 tgtactcggg ataaagatca gcttctccac tcagcaaggc ctcgtgcgga aggtttcctc
      421 ctaattcaaa tcgacgttgg accaccacac cgcgagcttc caacagttga gaaattatct
      481 cggcgaggag gatcgactcg gtaaagtctt tcgaagctat gacgacttga ccagcattag
      541 ttggtgttgt ggctgcgcgt tgactgcgta gagaggcaag cgttcccagg ccagcgaaca
      601 gtaggattac caccaacaag atcttctgac ttagcgccag accgcgatcc tttcgttcca
      661 gactaaagcg ttgttccagg agacccagga ggaagtccgc tacaagtgcc aggccagccg
      721 aagcgatagc accactcaga agaagattgt tgtcgtactg gcgcaagccc cggaatatat
      781 agactccaag tccgccggca cccacagcag ccgctattgt tgtaactcca accgcgatca
      841 ccgtcgcgat acggacgccg gtaataatca cgcccattgc gagcgggagt tcgacctgcc
      901 acagcatttg tctgtcagtc attcccatcg caatggcagc ttctcgaacg ttcggatcga
      961 ctccaagaat tccggtcacc gtgttgcgaa taatcggtag tagtgaatag agcacgagcg
     1021 ctaccagtgc cgttcgccat ccgataccgc cgataaacgg aagcggaatg agcagaccaa
     1081 acaaagcaag actgggaatg gtctgcatta tgttggcgag gccgagcact ggattgtgca
     1141 atttacggta acgagtgatg aggatgccgg tgggaacgcc gatcagaacc gcggcgagta
     1201 ttgaggcgga tacgagttgg atatgctgcc gtgtcagcaa aagcaactcg ggccagttat
     1261 gttgaaggaa ctcccagctc attggtcctg gtccgacact ttctccatct gcagagtttc
     1321 tatgtaagcg cgtgcttttg gattatcaga acgctcgaaa cccgcgggtg tttccaacag
     1381 cacgattttg ccggcgtcca tcaaagcgat gcgatcacca agcagcaacg cctctctaac
     1441 gtcgtgagtc accagaaccg cagtcttgtt cacacggcga gcaagttctg caaactcttt
     1501 ctgtacgctc gctcttgtca acgggtccaa cgcgccaaaa ggttcgtcca ggagaagcag
     1561 actcggatct ccggccaacg ctctggcaac accaactcgc tggcgttgcc caccggacaa
     1621 ttcgcgcgga tagcgatcga taaatctgtt gggctcaagc cccaccaatt gcagtagttc
     1681 acgagcacgt tcggttcttt tctgagcagg ccaggccaga agctcaagca caagacccac
     1741 atttctgcca atcgtgaaat gaggaaacag cccaccttcc tggatgacgt aaccgatatg
     1801 acgccgcagc tcgatggcat tccaactcat cgttgacttt ccttctacca aaacttcacc
     1861 tgaggtgggc gaaagtagtc tgttaataag tttaagagtt gttgttttgc cgcagcctga
     1921 ttcgccaagc aggaccagtg tttcaccgtt cgagacagtg agattcaggt ggtcgattat
     1981 tggcgctgca gcggttggta aggagaaggt ggcgtcccga aactcgacta ttattttgtc
     2041 ggggggagcc gtcatcaggc ggtctaactg gcgagtaaca gattgctgct aaatcgttct
     2101 tgtgagtcaa accatgttcg tgcacatttg aatcccgttg cttcggcgag tttcgcaatc
     2161 gagtcgagat catatttgtg ggaattctct gtatgaatca gttcgccggc gctgaactca
     2221 acttcgaggt caagcttttc aattctgact gtctgattgc gcaggctctc gatgtagatc
     2281 tctatgcgag agcttgactc gttgaagaac gcataatgtt tgaaagctcg tagatcaaag
     2341 gtcccaccga gttcacgatt gatgcgcgcc agaacgttca gattaaaagc ggacgtcaca
     2401 ccaagggcat cgttataggc agcttccagg atcgctggat ccttttttaa gtcagctcca
     2461 agcaacaggg cgtcgccact c
//
LOCUS       DASSFP010000309         4761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_230913, whole genome shotgun sequence.
ACCESSION   DASSFP010000309 DASSFP010000000
VERSION     DASSFP010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230913"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..1727)
                     /locus_tag="VFH91_06875"
     CDS             complement(420..1727)
                     /locus_tag="VFH91_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6862751.1"
                     /translation="MSTVETEVSQSESVRKHKEFLFPAVAMYYKEPLALVRGEGFHVW
                     DDQDNKYLDCFGGVLTVSVGHNNPRVSEAIIKQVQSIQHTSTLYANKPQSDLAEKLAQ
                     ITPGNLKKSFFTNSGTEADDTAVHAAKLATGRHEIVVLRHSYSGRSATALSAMGHATW
                     RPLPAQIPGIVHAPAPYCYRCPFKLTYPECGLACANDIEELIMTSTTGEIAAFMSEPI
                     LGVGGFVVPPPGYFERAVEITRKHGGLFIADEVQTGWGRTGKHWFGIEHWDVTPDIVT
                     SAKGLGNGVPIGITVATPEVADKYPGLTFSTFGGNPVSMAAALAVIKEIEDNDLRTNA
                     AEVGKYLRLRLEELKEKYASIGDVRGMGLMQGLELVKDRVTKEPAPEAVARVFEETKR
                     RHVLIGKGGLYGNVIRTGLMLNATRDHVDELITALDESFKAIS"
     gene            complement(1775..2326)
                     /locus_tag="VFH91_06880"
     CDS             complement(1775..2326)
                     /locus_tag="VFH91_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862752.1"
                     /translation="MSTRKCLLWTPALLLVLSSWQTSRAQPNRSVYTSLDAKQCRTLK
                     NDTSEAGDYLGRCKGVSGYSLLLAEGDLRQNLTVVTPSGSKHSLELWTVISGGFSSLG
                     SNAEWRVNKQGNKLVPVALIVRFNASEDPENSSKVSSYLAVTKISKSEICVTHKIGPG
                     PQANEDARRAADAAADKPCLKAR"
     gene            complement(2336..3814)
                     /locus_tag="VFH91_06885"
     CDS             complement(2336..3814)
                     /locus_tag="VFH91_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6862753.1"
                     /translation="MNVYRNFIGGKWVESTSTRTANNLNPANTDHVLGTIKQASRDEA
                     RAAVESAAEAFRTWRGTPAPSRGRIVTLAARLMEQHKEELAQLLTLEEGKTIAESRGE
                     LQRSINVAEFCAGESRRMNGETIPSELPLNFAYTLKQPIGVVACVTPWNFPVAIPIWK
                     IAPALVAGNTVVFKPASITPGTAVRITEIFEEAGIPPGVLNLVLGSGSEAGDEIINHP
                     AVKAISFTGSNGVGIRLYEQASRRGAKVQCEMGGKNPVIVLEDADMDLAVESTAQGAF
                     GSTGQRCTATSRAIVMDQVADKFVDRIVKRAQSMRIGAGADPTTEMGPSVDESQFKTV
                     LSYIDIGKEDGATLACGGEQAKGPGLDKGYFVRPTVFDHVTPDMRIAREEIFGPVLSV
                     LRVKDFDQAMQVANDCEYGLSSSIFTNDAARIFRFVDEIETGMTHINSPTTGGEAHVP
                     FGGTKGTGIGDREQGSTALDFYTELKVVYVDYTGRKREGNLY"
     gene            4057..>4761
                     /locus_tag="VFH91_06890"
     CDS             4057..>4761
                     /locus_tag="VFH91_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET6862754.1"
                     /translation="MHIVELLNSRVSQSPDKSFIFSEADGRSYTYLQFAKAVNRLAAL
                     LSGKGIKKGDVVSLLLPNSVEYVIAYFACWQLGALAGPINSLLKEHEMSYVISDSEAR
                     ALLVHTEFLPIIERIRSQLTTLQEVITLDNESDATQGIPNESTQASVVNSDDEAIIIY
                     TSGTTGKPKGCLLTHGNIVANARQISEWLHFGRNDRLLTIMPLFHMNAVSVTTMSALY
                     AGGSTVVSPKFSASRFW"
BASE COUNT         1104 a         1219 c         1185 g         1253 t
ORIGIN      
        1 ctttgcggat gagttctggt atgtggtggt tttagcggtt gaggtatgag ctgtcggtgt
       61 cgcataactg ttggagctgt tttcggattt cttagttttc agacaagccg tagccaataa
      121 ggcaaagact acgaatccgg ccaggaaaaa atcgtttctc ttaagtcctg atgtcatgga
      181 gcgtccttct cgcgtgtgta ttttcagaat ttaaaaagag cgccaggaat ggtaacaagt
      241 aattcgcccg ctgatgccct ttgatgaacg tgaagattcg aagcccagac agctgcgagg
      301 gaagtgtgtt ggatttcgca agaacaggta aaagacgctg ccgagggcaa gcggagcaag
      361 cacatggagg attgccgcgc gtccagttag tctcattcca gagattgaat aggggccatt
      421 tacgagattg ccttgaaact ttcatctaat gcggtgatca actcgtcgac gtgatctcgt
      481 gttgcattga gcatcaaccc ggttcgaatc acgttgccgt atagaccacc cttaccaatt
      541 aagacgtgcc ggcgcttggt ctcttcaaat actcttgcca cggcctctgg cgcaggttct
      601 tttgtgacgc ggtcttttac gagttccagg ccttgcatta gacccattcc ccgcacgtcg
      661 ccgatactag cgtacttctc tttaagctcc tcgagccgca gtcgcagata tttgcccact
      721 tcggctgcgt tggtcctcag atcgttatcc tcaatctcct ttatgaccgc taacgcggct
      781 gccatcgaga caggattacc gccaaaggta gaaaaagtta agccgggata tttatccgct
      841 acctcaggcg tagctacagt gataccaatc ggcacgccgt ttccaagtcc tttagctgaa
      901 gttacgatgt ctggagttac atcccaatgt tcaataccaa accaatgttt gccagtacgt
      961 ccccaacctg tttgaacttc atcagcaata aataagccgc cgtgcttccg cgttatctcg
     1021 actgcgcgtt cgaagtaccc tggcggagga acgacaaaac cacccacacc aagaattggc
     1081 tccgacataa acgcggcgat ctctccagtg gtagacgtca tgattaattc ttcaatgtcg
     1141 tttgcgcatg cgagtccaca ttcgggataa gtgagtttga atggacagcg atagcaatac
     1201 ggcgccggag catgaacaat tcctggaatt tgtgcaggca aagggcgcca cgttgcgtga
     1261 cccatcgctg acaatgccgt ggctgaacga ccgctataac tgtgacggag tacaacgatt
     1321 tcatggcgtc cggtagctag ctttgcagcg tgaactgcag tgtcgtcagc ttccgtgcca
     1381 ctgtttgtaa aaaatgactt ttttaagttt ccgggtgtga tttgagcgag tttctcggct
     1441 aaatcagatt gcggtttatt ggcgtaaagc gttgaagtgt gctgaatcga ttgaacttgc
     1501 ttgataatcg cttcgctgac tcgtgggtta ttgtgcccga cgctaactgt cagcacgccg
     1561 ccaaagcaat caagatattt attgtcctgg tcgtcccaaa catgaaaacc ctccccgcgg
     1621 accagtgcca gcggttcctt gtagtacatc gcgacggcag ggaaaagaaa ctccttgtgc
     1681 ttgcgaacac tttccgattg cgacacttcg gtttcaacag tactcatttt tttgttttca
     1741 ctaataaaag atccgagttc gtttccaaag ccaactaccg ggccttcaga caaggtttat
     1801 ctgcggctgc gtctgcggcc cgacgagcat cctcattcgc ttgtggtcct ggaccaattt
     1861 tgtgggtgac gcaaatttcg gatttcgaaa ttttggtcac cgccagatat gacgagacct
     1921 tactagaatt ctccggatcc tcgctggcat tgaatcttac gatcaaggcc accggcacga
     1981 gtttgtttcc ctgtttgttg actcgccatt ccgcattcga gccgagactc gaaaagccgc
     2041 cacttatcac agtccataac tcaagagagt gtttggagcc cgagggtgta accactgtca
     2101 gattttgtct aaggtcgccc tcggcgagca gaaggctata tccactgact cccttacatc
     2161 ggcccaggta atcgccggct tctgaagtgt catttttcag tgtgcgacat tgctttgcat
     2221 ccagactcgt ataaacactt cggttaggct gggcccggct ggtttgccac gacgataaaa
     2281 ccagcaacag tgcaggcgtc cataaaaggc attttcgagt gctcatgacg gcccttcaat
     2341 aaaggtttcc ctctcgttta cgtccagtgt aatcgacata aacgactttg agctccgtat
     2401 aaaagtccag ggcagttgaa ccttgttcgc ggtcgccgat accggtgcct ttcgttccgc
     2461 caaatggaac atgcgcctcg ccgccggttg tgggcgagtt gatatgagtc attccggttt
     2521 cgatctcatc gacaaaacga aagatacgag ctgcatcatt ggtgaagatt gaggatgaaa
     2581 gtccatattc gcagtcgttg gcaacctgca ttgcctggtc gaagtctttc acgcgaagta
     2641 ccgaaagaac cggaccaaag atttcctcac gggcgattcg catgtcgggc gtgacgtgat
     2701 caaaaaccgt gggccgcaca aagtatcctt tgtcgagccc aggtcctttg gcctgttcac
     2761 ctccgcaggc gagtgttgca ccatcttctt ttccgatgtc gatgtagctc aggactgttt
     2821 tgaattgact ctcgtctacg cttggaccca tttcagtcgt cggatccgca cctgcgccga
     2881 tacgcatcga ttgcgcgcgt ttgactatgc gatccacaaa tttatcggcg acctggtcca
     2941 tgacgatagc gcggctggtt gcggtacatc tttgtccggt tgagccaaat gctccttgcg
     3001 ctgttgactc aacggcaaga tccatgtccg cgtcttccag aacgatgacc ggatttttac
     3061 ctcccatttc gcattgaacc ttggcgccgc gtcttgaggc ctgttcgtac agtctgatgc
     3121 ccaccccgtt cgaaccggta aacgagatcg ccttaaccgc gggatgattt atgatttcgt
     3181 ctccagcttc cgatcctgat cctaaaacga gattcagaac ccctggagga ataccagctt
     3241 cttcgaagat ttcggtgatg cgaactgctg tgccaggagt gatcgaagcc ggcttgaaga
     3301 caaccgtatt acctgcgaca agagctggcg caatcttcca aatcggaatc gcgacaggaa
     3361 aattccacgg cgtaacacag gcaacaacac cgattggctg tttgagcgtg tacgcaaaat
     3421 ttagaggtaa ctctgaaggg attgtctcgc cattcatcct tcgcgattcg ccggcgcaga
     3481 actccgccac gttgattgac cgctgcaact ctccacgaga ttcggcgatt gttttaccct
     3541 cttcaagagt tagtaattgg gccaactctt ctttgtgctg ttccattaga cgagcggcca
     3601 gcgtaactat tcgtccacgg gaaggcgccg gagtgcctcg ccaggttcga aatgcttctg
     3661 cagccgactc aacagccgct cgagcttcat ctcgactggc ctgttttatt gttccaagca
     3721 catgatctgt gttcgcaggg ttaagattat tggctgtgcg ggtagacgtt gattcaaccc
     3781 acttgccgcc gatgaaattg cgatatacgt tcatttgact ttaaaaaaag cgtggctcat
     3841 ccgactccac acccaggaag gaatgttttg tacctgatcg ggaattaaag cttaacggcg
     3901 ggtaaatata tcacgttctg ctgattggat tgagtcgaat tgagcttcgg gagttgatgt
     3961 cgtaactgat gccgacgagt tcataggtcg cactcaatgc cggccgtgat attgttggcg
     4021 cgatcttaaa taagaacttc tcaatccgat tgacccatgc atatcgtaga gctacttaat
     4081 tcaagagtaa gtcagtcgcc agacaagtcg ttcattttct ccgaggccga tggacggtcg
     4141 tacacgtatc tccaatttgc taaggccgtg aatcgactag cagcgttact gtcaggtaag
     4201 ggaatcaaaa agggtgatgt tgttagcctt ttgctgccga acagcgttga gtacgttatc
     4261 gcgtacttcg cttgttggca gctcggagct ttagctggcc caatcaactc acttctcaaa
     4321 gagcatgaga tgagctatgt catctctgat tctgaggcca gagctttgtt ggttcatacc
     4381 gagttcctac ccatcatcga aagaatccgc tcacaactga caacgcttca ggaggttatc
     4441 actttagaca acgagtcaga cgccacccag ggtatcccta acgagagtac tcaggcgtcg
     4501 gtcgttaata gtgatgacga ggcaatcatc atctatacgt cgggcacgac tggtaagcca
     4561 aagggttgtt tgcttactca tggcaacatt gtggccaatg ctcgtcagat cagtgagtgg
     4621 ctgcacttcg gccggaacga tcgtttgttg acgatcatgc cgctctttca catgaacgca
     4681 gtctcggtga caacgatgtc tgcgctctac gcgggcggct caaccgtcgt gagtcccaag
     4741 ttttccgctt ctcggttctg g
//
LOCUS       DASSFP010000310         4367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_85068, whole genome shotgun sequence.
ACCESSION   DASSFP010000310 DASSFP010000000
VERSION     DASSFP010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85068"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..866
                     /locus_tag="VFH91_06895"
     CDS             12..866
                     /locus_tag="VFH91_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET6862755.1"
                     /translation="MTQQYGDNAPLSYNKYLRVQDLIELQNCLSDPVHHDELLFITVH
                     QAYELWFKQILHELDAAMALLNEDRLKLATRAINRIVDIEKLLISQIHILESMTPISF
                     LSFRDELNPASGFQSMQFREIEFSSGLKDERILREFSSDAYAFGRLQKRFEGDTLADA
                     FYRVLSKRGLKAPVDAGTDQAQATENYHERTHAILEILTHFEDRYEEFQLAEALMEHD
                     EYFSLWRSHHIKMVERMVGAKRGTGGSEGIGYLKTTLDKKFYPELWEARTYLDSKHRG
                     SECPFAGH"
     gene            867..1226
                     /locus_tag="VFH91_06900"
     CDS             867..1226
                     /locus_tag="VFH91_06900"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6862756.1"
                     /translation="MERGDCSPFYLRVEKDRRSAPRVRVKLPARWEGVLSREEATITD
                     LSRNGCFVLSGGKIEHKELIWLEISLPEGPVSFWAEVVDAPPEIGFAVRFNSSSDEDN
                     ERLARYIESVFATRVPE"
     gene            1269..2843
                     /locus_tag="VFH91_06905"
     CDS             1269..2843
                     /locus_tag="VFH91_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET6862757.1"
                     /translation="MNVTFPERFNMAQYFLYHNVDEGREKKVCLYFQERSWTYGETVD
                     FSNKAGNALRELGIEMEDRVLIVLPDCPEFVWTWFGAARIGAVITMVNPLLPASDYKY
                     YLDYTRARVAVIHESLLPAFSEAALSSPYLRAVLVVGDVQPFAPAKTKWQRFDDLVSS
                     CESECTPADTRRDDIAIWLFTSGSTGHPKGAVHLQHDLPFNTEVFAKAAMGVSSDDLT
                     VSVPKLFFGYATGTNLLFPFAVGGATALFSERSTAEKLFEVIKRYRPTILTTVPTMIN
                     SMLNLSDSQADDLKSLRFCYSAGEALPIELYERWMERFGIDICDGIGSAEMFHIYITN
                     RPGDIKPGSLGKIVAGYEARIVNADNEEVKTGEMGTLRIKGDSAALCYWNAHEKSKET
                     FAGDWCTTGDQFHVDEQGYYWYHGRTDEMLKVSGVFVAPAEIENCLLQHQAVLECAVV
                     GHQDKGLVKPKAYIVLRDEFIQSEGLAEEIKQYVKTRIAVYKYPRWIEFVDSLPKNDR
                     GKIDRKRLKTDVQSTG"
     gene            complement(3080..>4367)
                     /locus_tag="VFH91_06910"
     CDS             complement(3080..>4367)
                     /locus_tag="VFH91_06910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862758.1"
                     /translation="RRLEESNVDVLLCDINMPRIDGMELLRRVHERPNPPEVIMLTGQ
                     ATVETAVEAMKLGAYDYLTKPYSINELDIRVKQAADKRRLRVDNVRLREQLARQSALP
                     EIVSVSELMKEAIRLIERVAPSEASVLITGESGTGKELVAHAIHRLSGRADASFVDLN
                     CAAFQESLLESELFGYEAGAFSGAKGRKLGLIELADGGTLFLDEVTELPAQLQAKLLR
                     AIETRTFFRVGGVRKVEVNVRIVAATNRNLDAVLTEGVFRSDLLYRINGFQISLAPLR
                     ERPEDIEPLTKYLLKQIAGTTPPVLTDDAFAALRSYGWPGNVRQLKNCLERAVILSNN
                     GRITLSELPPEVIRPNVSLPAIQPVTASAAPSNTNASAPVSSSAPGSLREVERQQILA
                     ALEQTGWHRGKTAEILGISPSTLYRRLRDYDLERRR"
BASE COUNT         1109 a         1097 c         1086 g         1075 t
ORIGIN      
        1 cctgattaat catgacacag caatatggtg ataacgcgcc cctgtcgtac aacaaatacc
       61 tgagagttca ggatttgata gagctgcaaa attgtctttc agatccagtt catcacgacg
      121 aactgctttt cataaccgtt catcaggcgt acgagttgtg gtttaaacag atccttcacg
      181 aactcgatgc ggctatggca cttctcaatg aagaccgatt gaagctcgca accagggcaa
      241 tcaatcgcat agtagacatc gaaaaactgc ttatcagtca gattcacatt ctcgaaagca
      301 tgactccgat cagctttctc tcattcaggg acgaactgaa tccagcaagc ggttttcaat
      361 caatgcagtt tcgtgaaatt gagttttctt cgggactaaa ggacgaaagg atattgcgcg
      421 agttttcaag tgatgcttac gcgtttggcc gactgcaaaa aaggtttgaa ggtgacactc
      481 tggctgacgc cttctatcga gtactttcga aacgcggttt aaaggcccca gtcgacgcag
      541 gcaccgatca agcacaggcc acagagaatt atcacgagcg aactcacgct attcttgaga
      601 tcttgactca ctttgaagat cgttacgagg aattccaact cgcagaagct ttaatggaac
      661 acgacgagta tttttctctg tggcgatcgc accacatcaa gatggtcgag cgaatggtag
      721 gtgccaaacg tggaaccggt ggatcggaag gcattggtta cctcaaaacc acgcttgata
      781 aaaagtttta tccggaattg tgggaagctc gaacctacct cgattcaaaa caccgcggta
      841 gtgagtgccc gtttgcgggc cactaaatgg agcgcggcga ctgctcgccg ttttatttga
      901 gagtggaaaa agatagacga agtgcaccac gcgttcgagt gaaattgccg gctcgatggg
      961 aaggtgtact ctcacgcgaa gaagcaacca tcaccgacct aagtcgcaac ggttgttttg
     1021 tattgtcggg cggcaagatc gaacacaaag agttgatctg gcttgagatt tccttgccag
     1081 aggggccagt ttctttctgg gctgaggtgg tagatgctcc accagagatc ggttttgcag
     1141 tgcggttcaa ttcaagcagc gacgaagata acgaacgcct ggctcggtac atcgaaagcg
     1201 tttttgcgac cagggtgccg gaataacggc catcgcctcc tcaccaatcc taaatcgagt
     1261 caacaattat gaacgtcaca tttcccgagc ggttcaacat ggctcagtac ttcctttatc
     1321 acaacgttga tgagggtcgg gaaaaaaaag tctgtcttta ttttcaggaa cgcagttgga
     1381 cttacggcga aacggtcgat ttctcgaata aagccggcaa cgcactacgt gaacttggaa
     1441 tcgagatgga ggaccgtgtc ctcatagttc tcccggattg tccggagttt gtttggacct
     1501 ggttcggagc ggcacgcatc ggcgcagtta taacaatggt gaatcctctt ctgcctgcgt
     1561 ccgattacaa atattacctc gattacacgc gagcccgcgt cgctgtcatt catgaatcgt
     1621 tgctgcctgc tttttccgag gctgctttgt cttcacctta tcttcgggct gttttagttg
     1681 taggcgatgt acaaccgttt gccccagcaa agactaaatg gcaacgattc gacgacctcg
     1741 tgtcctcttg cgagtctgag tgcactccgg ctgatacgcg gcgcgacgat attgcaatct
     1801 ggcttttcac ctcaggctca accggtcatc caaaaggcgc cgttcatctc cagcacgatt
     1861 tgcctttcaa cactgaagtg ttcgcgaagg ccgctatggg agtttccagc gatgatctga
     1921 ccgtgtcagt gcccaaactc ttcttcggct atgcgacagg aacgaacctg ttatttcctt
     1981 ttgccgttgg tggagcaacc gcactgttct cggagcgttc aaccgctgaa aaattgttcg
     2041 aagtcatcaa gcgttatcga ccgacgattc tcacgacggt tccgacaatg atcaattcaa
     2101 tgctcaatct ttcggatagt caggcagacg atttgaagag tttgcggttt tgttattccg
     2161 caggcgaggc gctaccgatc gaactctacg agcgttggat ggagcgtttc ggaatagaca
     2221 tctgcgatgg catcggctct gccgagatgt ttcatatcta catcacgaat cgtcctggcg
     2281 atatcaagcc cggaagcctc gggaaaatcg ttgccggata cgaagcccgg atcgtaaacg
     2341 cagataacga agaagtgaag accggcgaaa tgggaacgct tcgtatcaaa ggtgattctg
     2401 ctgccctttg ctactggaat gcacacgaga aatccaaaga aacctttgct ggtgactggt
     2461 gtaccactgg agaccagttt cacgtggatg agcagggcta ctattggtac cacggaagaa
     2521 ctgatgaaat gctcaaagtg tcgggtgtat ttgttgcgcc tgctgaaatc gagaattgtc
     2581 tcttacaaca tcaagcggtc cttgaatgtg cggttgtggg ccaccaggat aaaggtctcg
     2641 taaaacccaa agcatatatt gttctacgtg acgaatttat tcagagtgaa gggctggcgg
     2701 aagagataaa gcaatatgtc aaaacccgga tagccgtcta taaatacccg cgttggattg
     2761 aattcgtcga ttctctacct aaaaacgatc gtggcaagat tgatcgcaag cgtcttaaaa
     2821 ccgacgttca gtcgacagga tgatttcctg gaattgcccc gcgatcccga gtttcctata
     2881 gatagtcgca acgatgtacg aattcggttg taccggttgg gcttcctggc cacgacagga
     2941 ccatgcgtaa gcctggacca tcgttccgac cgaactagta caaccgaagc ctcggtacag
     3001 atcgtcacaa ttataaaaaa actaaaacgg cgcattggac ccagaccaat gcgccgttga
     3061 agtgtgattt gacctcacct cagcgtcgcc gctccaagtc gtaatccctt aatcgacgat
     3121 acagtgtcga tggagagata cccaggattt cagcagtctt tcctcgatgc caaccagttt
     3181 gttcgagagc tgcgagaatc tgttgtcgtt ctacttcgcg aagcgaaccg ggcgccgagg
     3241 acgaaaccgg tgccgatgca ttcgtgttgg acggtgccgc tgaagctgtg accggttgaa
     3301 tggcaggaag gcttacgttc ggccttatta cttccggagg taactcggac agagtaatac
     3361 gtccgttatt cgaaaggata acggctcttt caagacaatt ctttaactgc cgtacattcc
     3421 cgggccaacc ataggaacgc agggccgcaa acgcgtcgtc agttagaacg ggtggggttg
     3481 tgccggcaat ctgctttaat aagtacttcg tcagcggttc gatgtcctct ggtctttcac
     3541 gcagaggcgc aaggctgatt tggaagccat taattcgata gagtaggtct gacctaaata
     3601 cgccctcagt aagaacggca tccagattcc tgttagtcgc agctacgata cgaacgttaa
     3661 cctcgacttt gcgtacgccg cccactctaa aaaatgtgcg agtttcaatt gcacgcagca
     3721 actttgcctg gagttgcgca ggaagctcag ttacctcatc aagaaacaac gttccaccgt
     3781 cagccaactc aatcaagccg agcttccgac ccttggcgcc cgaaaaggca ccagcttcgt
     3841 aaccgaagag ttctgactcg agtaacgatt cctgaaaagc cgcacagttc aaatctacaa
     3901 acgaggcatc ggcccgtccg gacaaacgat gaattgcatg ggcaactaac tctttgccgg
     3961 taccggactc acccgtaatt agcactgatg cttctgatgg tgctacgcgt tcaatcagtc
     4021 tgatcgcttc tttcatcaac tccgaaacag atacgatttc cggtaacgct gattggcggg
     4081 ccagttgctc tcgtaaccga acattgtcga ctcgcaaccg gcgcttgtct gccgcctgtt
     4141 taacgcgtat atcaagctca ttaatactgt aaggcttggt cagatagtca taggcgccga
     4201 gcttcatcgc ctcaacagca gtctctacag ttgcctggcc cgttaacatg atcacctcgg
     4261 gaggattggg ccgttcgtgt actctgcgca acaactccat gccatcaatc cgaggcatgt
     4321 tgatgtcgca caacaacacg tccacattgc tttcttccag gcgtcgc
//
LOCUS       DASSFP010000311         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2025487, whole genome shotgun sequence.
ACCESSION   DASSFP010000311 DASSFP010000000
VERSION     DASSFP010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025487"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..2049)
                     /locus_tag="VFH91_06915"
     CDS             complement(178..2049)
                     /locus_tag="VFH91_06915"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6862759.1"
                     /translation="MQARLAAISGKLKGAIITIKDDSFVIGREVAANLCIADASVSRR
                     HSLIEKQGDDLVISDLESLNGTFVNDVPVRTRVLEHGDRVRIGESQFLYLAHEAAVTS
                     KSSLVRLDGTKVMSGATLQVRFDDAFYLMARDLSALMKISATINAIRGLESLQARLLE
                     LLFEVVPAQYGAIVLVDDETLGNGAVFGLDRTHGKDCTVTVSATIAQQVLREGVALLA
                     SNSLGSDFASDSLVESATASVLCVPLIMLDRKLGVLYLATSVSDDQFNKDHLQLVTAI
                     SGIAAVAIENARRFEWLESENQRLLADVNIEHNMIGESGAMQRVYHFISKVAPTDSTV
                     LVEGESGTGKELAARAIHRNSKRADKPFIAVNCAALAESLLESELFGHEKGAFTGAQS
                     QKKGRLEIADGGTLFLDEIGELSPFLQVKLLRVLQEREFERVGGNVSIKVDIRLIAAT
                     NRNLEEAIELGQFRQDLFYRLNVVSFEMPALRQRREDIALLASYFVDKYGAKCNRKLQ
                     GISAEARERLSAYDWPGNVRELENAIERAVVLGTTDIILPDDLPEAILERHVLNVDHP
                     ASYHDAVTHTKKQIILQAIEQTHGNFTDAAKALGVHPNYLHRLIRNLDLRSHIKTRP"
     gene            2353..2814
                     /locus_tag="VFH91_06920"
     CDS             2353..2814
                     /locus_tag="VFH91_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862760.1"
                     /translation="MIKQITSHLRIVGLVISFALVAGSSAVYGQGSKRQVADIPFQFQ
                     VGNTTLPAGEYSVTPNSSTGETLRISSRSTDATVVRMSSPVAQRDPASKGRLVFHQYG
                     NEYFLTEVWTAGFSNGRKLTKSGRERALERDASLIGRASSQKPVEVVITLQ"
BASE COUNT          756 a          816 c          718 g          789 t
ORIGIN      
        1 caaccatcct tcactgggtc agttcgattt gttcctgacg atgggaaaaa acgataacgc
       61 cgatctgttt tacgaagcgg ttatcaatca ccttagttaa cgattgtgca ttggcaaata
      121 attctacaaa accctgcaga aaatttattg gatggaaaca ggatcactgt ggcccaacta
      181 cggcctggtt tttatgtgac tgcggagatc cagattcctg attagacgat gaagatagtt
      241 cggatgaacc ccgagtgctt ttgccgcatc cgtaaaattt ccgtgtgttt gctctattgc
      301 ttgaagaatg atctgcttct ttgtgtgagt gacagcatca tgataactgg cagggtgatc
      361 aacattcagc acatgccgct caaggatggc ctcgggcagg tcgtcgggca aaattatgtc
      421 ggtggttcct aaaaccaccg cccgttcaat tgcattttcc agttcgcgca catttccggg
      481 ccaatcgtac gccgacaatc gctcgcgtgc ttccgcagag attccctgta atttccgatt
      541 gcatttagcg ccgtacttgt cgacgaagta acttgccaaa agcgcaatat cctcgcggcg
      601 ctggcgcaaa gccggcattt caaaggacac aacgtttaac cgataaaaga ggtcctgtcg
      661 gaactgtcca agttcgatgg cttcctccag attcctgttt gtcgcagcaa tcaacctgat
      721 gtctacctta atcgatacgt tgccgccaac tctttcaaac tctcgctctt gcagaacacg
      781 aagtaacttg acctgcagaa aaggcgagag ctcaccgatc tcatcaagga acagcgtgcc
      841 gccgtcggca atctccagcc ggcccttctt ctgactttgt gcgccagtaa atgctccctt
      901 ctcatgtcca aataactcgg attccagcaa tgactcggcc aaagccgcac aattcacggc
      961 aataaatggt ttgtcagctc tcttactgtt acggtggatc gcacgtgcag ctaattcttt
     1021 gccggttcca ctctcacctt caaccagaac ggtagagtca gtgggcgcaa cttttgaaat
     1081 gaagtgatat acccgttgca tggcaccact ttcaccaatc atgttgtgct cgatatttac
     1141 atcagccaac aatcgctggt tttcgctttc gagccattcg aaacgtcttg cgttttcgat
     1201 tgcaacggca gcaattcctg agatcgcggt aaccagttgt aggtgatcct tattgaactg
     1261 gtcatcgcta acactcgtag cgaggtataa gacgccgagt ttgcggtcca acatgattaa
     1321 cggaacgcaa agaaccgacg cggtagctga ttcaaccagg ctgtcactgg caaagtccga
     1381 acccagtgaa ttactcgcaa gcagtgcgac accttcacgc agcacttgtt gagcaattgt
     1441 tgcgctaacc gtgacagtac aatcctttcc atgagttcga tcgagaccga aaaccgcgcc
     1501 gttccccaat gtctcatcgt cgactagaac gatcgcacca tattgtgccg gcacaacttc
     1561 aaacaggagc tctagtagtc gcgcctgaag cgattcaagg cctcgaatgg cattaattgt
     1621 cgcgctgatc ttcatcagcg cgctcaggtc acgggccatg agatagaaag cgtcgtcgaa
     1681 cctgacttgg agtgtcgccc cactcatcac tttggtccca tcaagtctta ctagactgga
     1741 tttggaagtg acagcggcct catgtgcgag gtagagaaat tgtgattcgc cgatgcggac
     1801 tcgatcccca tgttcgagca ccctcgttct tactggcacg tcatttacga aggtgccgtt
     1861 caggctctcg agatcgctta tgacaaggtc gtcgccctgt ttttcgataa gggaatggcg
     1921 gcgtgaaacc gaagcatccg ctatacacag gttggcggct acttcgcgac cgatgacgaa
     1981 cgagtcgtcc ttgattgtta ttatcgctcc cttaagtttt ccggatatgg ctgcgagtcg
     2041 tgcctgcatt tttcttcgtt gagaggctct ttctgatctc ctcaaagtct acgcgtctaa
     2101 cggcattccg gcaacaacct tatgaccgtc gatcataatg ctatccaacc agttagctga
     2161 actatctctt tagatagtta tcaggacccc tgggaggttc cagaatctgt gctgaactca
     2221 accctcccat atctacatac cctttctaaa gggtttttat ttcatttcat ccacacttat
     2281 cgagtggcac gatcgtagca aggggactcg cgtaacaagg ccacgccgcc gcccagacaa
     2341 aggagaaaga caatgatcaa gcaaattaca tctcatctta gaatcgtagg tctagtgatt
     2401 tcgttcgcac tcgttgccgg ctccagcgcc gtctatggac aaggatccaa gagacaagtt
     2461 gcggacattc cgttccagtt tcaggtagga aatacaacct taccagccgg cgaatatagc
     2521 gtcaccccaa actcatcgac tggtgagacg cttcgcatca gttcacgttc aaccgacgcc
     2581 accgtagtca gaatgtccag cccggtggcc cagcgtgacc cggccagcaa aggtagactg
     2641 gtgtttcacc agtatggcaa tgagtatttc cttaccgaag tttggaccgc tggtttttca
     2701 aacggccgaa agcttacgaa gtcaggacgc gaacgcgctc ttgaacgcga cgcctcgttg
     2761 attggaaggg caagtagtca gaagccggtt gaagtggtta ttacgttgca gtaagtgcag
     2821 ggctgtccgc gcgggagacg tgccagcggt aactggagtc gtacacgtct cctttataag
     2881 aaaccaggac ggcaatccaa agcccccgga ttgccgtcct ggtttttttg atcggaatag
     2941 gtcctatagg tcgtataggt cctataaaaa cccaacctcg actcctccaa ttaatgaaca
     3001 attctccaat tattattcgg ctcaaccggg atcgttttgt gtttctcgac ccagtcgata
     3061 atcaattctc taatttcct
//
LOCUS       DASSFP010000312         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_232264, whole genome shotgun sequence.
ACCESSION   DASSFP010000312 DASSFP010000000
VERSION     DASSFP010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232264"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFH91_06925"
     CDS             complement(<1..615)
                     /locus_tag="VFH91_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862761.1"
                     /translation="MKIAAIDIGSNSIKLIVVDAAASNSFAVLTREREVVRLGHDTLL
                     KGYISRAAIERSTECIKRFRSIAEARGAEQIVAIATASVREANNSANFIKAVAQRTGV
                     QVEVLSGIEEARLIGLAASHGCARPGTTTINVDIGGGSTELSVFNDGVAATLLSVKLG
                     AVGLTERHLHHDPPTANELSELRAEIRAALARPARELREQQWKDA"
     gene            complement(612..1313)
                     /locus_tag="VFH91_06930"
     CDS             complement(612..1313)
                     /locus_tag="VFH91_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862762.1"
                     /translation="MALLRTWLESRGHAVLDTGMTRSVLAGRGIKEAKTGHTLGRITM
                     QLFYATDFIDRLENEMLPALRSGFVVLTDRYIYSAMARAEVRGIDSAWIRSIYGMALI
                     PDAVFYLRIQSPQELVERVLSSGRGFDYWESGMDLYLGEDLYDSFVEYQKRLLTVFDR
                     MSTEYQFHVVNASRSVRTVASDLRRAVAKLIDEPRTSGEIAKPVKQSADATTTQPTTS
                     VSPLSSPPESKKVIM"
     gene            complement(1420..2097)
                     /locus_tag="VFH91_06935"
     CDS             complement(1420..2097)
                     /locus_tag="VFH91_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HET6862763.1"
                     /translation="MISYGKHRFPGKLFVVEGTDGSGKSTQMALLYQWLKAEGYPVFF
                     SEWNSSPLVKDTTRRAKKRHLFTPATFSLLHATDFADRTERDIIPPLKAGAIVLADRY
                     IYTAFARDVARGCNRDWVRQVYSFAVKPTVAFFFRAPLDIAIDRIISGRPSLKYYEAG
                     LDLGWADDAEESFTIFQGRILEEYDRMVQEFGLTVIDASRSIERQQREMRMLITEHLK
                     KRRSRSN"
     gene            2163..2651
                     /locus_tag="VFH91_06940"
     CDS             2163..2651
                     /locus_tag="VFH91_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6862764.1"
                     /translation="MRSLYLLRHAKSSWKDDSIADVDRPLKKRGRAAAERVGKTLGAE
                     RLSSPVLISSPALRTRETTDIVLKAGHLKVIVSYDERIYEADLASLLRVIAEVAADKT
                     ELILVGHNPGMEYLLDFLTGPGQPMPTAALAKIILETASWDSVERGDGRLEWLKTPAD
                     LE"
     gene            3088..>3341
                     /locus_tag="VFH91_06945"
     CDS             3088..>3341
                     /locus_tag="VFH91_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862765.1"
                     /translation="MRRKIKLFGVQLVNILVTWRELALSPEGAGKPRTDAATTTTPVQ
                     YLGGIEMQPSSRTLRPLSSHLASVVLVSLSCLIINSHAET"
BASE COUNT          790 a          850 c          864 g          837 t
ORIGIN      
        1 agcgtctttc cattgttgct cgcgtaactc tcgcgctggt cgcgctagcg cggcgcgaat
       61 ttcagcacgt agctctgaca gttcattcgc cgttggtgga tcgtggtgaa gatggcgttc
      121 agtcaaacca acggcgccaa gcttcacgga taacaacgtc gctgctactc catcgttgaa
      181 gactgaaagc tctgttgaac cacccccaat gtcgacgttg atcgtcgtgg taccgggcct
      241 tgcacaccca tgcgacgcgg cgagtccgat caaacgtgct tcctcaattc cagaaagcac
      301 ctcgacctga actccggtgc gctgcgctac ggctttgatg aaattggctg aattgtttgc
      361 ctctcggact gaggcggtgg caattgcgac aatctgttcg gcaccgcgag cttcagcaat
      421 tgaacgaaac cttttaatgc attcggtcga tcgttcgatg gcagcccggc tgatgtaacc
      481 cttcaacaag gtgtcatggc cgagacgtac tacttcgcgt tccctggtca gaactgcgaa
      541 tgagttgctc gctgctgcgt ctacgacgat gagtttgatt gaatttgagc cgatatcaat
      601 tgcagcaatt ttcacatgat gacctttttg ctctcaggtg gactggaaag gggtgacacc
      661 gatgttgtcg gctgggtggt tgttgcgtct gcgctctgct tgacaggttt agcgatctca
      721 cctgaagtcc ttggttcatc gatgagtttg gcaacagctc gtcttaaatc cgatgcgacg
      781 gtccgaacgg aacgtgacgc gttgaccacg tggaactgat actcagtact cattcgatca
      841 aacacagtta acaaacgctt ctgatattct acgaagctgt cgtaaagatc ttcgcccaga
      901 tataggtcca tgccggattc ccaatagtca aacccgcggc ctgagcttag cacccgttca
      961 acgagttcct ggggtgattg aatgcgaaga tagaaaacag cgtccggaat gagggccatt
     1021 ccatagatcg accgaatcca tgctgagtcg attccgcgaa cttcagctcg ggccattgcc
     1081 gaatagatat atcgatcagt tagaactaca aatcccgatc gcaaagccgg caacatctcg
     1141 ttttcgagtc tgtcgatgaa gtcagtcgcg taaaagagct gcatcgtgat gcgacccagt
     1201 gtgtgtccgg tcttcgcctc tttgattcct cgtccagcga gaacggaacg agtcattccg
     1261 gtatccagga ctgcatgtcc tcgcgactcg agccaggtcc ggagcagggc gatatgcgtc
     1321 gaacggccga cgccatcggt gccttcaatg acaatgagtt tgccgctgta gtcttcgtgt
     1381 tcgacgccgg gtaaaggcgt gccgtaaaag tgtcgcgtac taattagacc tggaccgcct
     1441 tttctttaag tgttccgtaa tcagcattcg catctcacgt tgttgacgtt caatggagcg
     1501 actcgcgtcg ataacggtta gaccaaactc ttgaaccatt cggtcatact cttcgagaat
     1561 cctgccctga aagatcgtaa aactttcttc tgcatcgtct gcccatccaa ggtcaaggcc
     1621 agcctcgtag tactttaagc tcggacgccc tgagatgatg cgatcgatcg caatatctaa
     1681 aggagcacga aagaagaacg ctacagtcgg tttcacggca aaggagtaga cctgacgaac
     1741 ccagtctcga ttgcaaccgc gagcgacgtc gcgagcaaat gccgtataaa tataccggtc
     1801 cgcgagaacg atggctcccg cttttagcgg tggaataatg tcgcgttcag tgcgatccgc
     1861 aaaatcggtt gcatgaagca atgagaaagt ggccggtgtg aacaggtgcc gtttcttggc
     1921 tcgtcttgtt gtatccttca cgagtggcga cgaattccac tcggaaaaga aaacaggata
     1981 gccttctgct ttcagccatt ggtacagaag tgccatctga gtagatttac cgctgccgtc
     2041 ggtgccttca acgacaaaaa gtttgccagg aaaacggtgt ttgccatagg aaatcatcgg
     2101 cgcagaccct aggttattag tgttacagga acgtaaacgt ccattggacc actcagggaa
     2161 ttatgagaag tttgtacctc ttaagacacg ccaaatcaag ttggaaggac gattctatcg
     2221 cagacgtcga tcgtccattg aagaaacggg gacgagcagc cgctgaacgc gttggcaaaa
     2281 cgttaggtgc ggagcgactc tcgagcccgg ttttgatcag ttcacccgcg ctccggactc
     2341 gcgagactac tgacatcgtg ctcaaggctg gtcacttgaa ggtaattgtc tcttacgacg
     2401 agcgtattta tgaagcagat ctcgcaagcc ttttaagggt tatcgcagag gttgccgccg
     2461 ataagacgga gcttattctg gtcgggcaca acccgggtat ggaatacctt ttggattttt
     2521 tgacggggcc ggggcagcca atgccgactg ctgcgctggc aaagataatc ctggagactg
     2581 cgagttggga ttcagttgaa agaggagacg gccgattgga gtggttaaaa acgccagcgg
     2641 acctcgaatg atgactgacc cttaccgttc gctgacaaac tcgcccgacg gcgttcgctt
     2701 ttgagttgcg agatgtttgc tcaagctttg ataaaaacat gtgaggcatt cagcgttatc
     2761 gtgtggctgt ttcgtctcgc gaattttaga accacaccaa acgcaaagta ttgaatactt
     2821 tcgcttcgct tgtatctttt tcaaaatcat taacctcctt cccttgttcg acctaatctt
     2881 taccgtcgtc atgtgccact gaagttaacg gcgattgacg atcagattaa atttccgtcc
     2941 tcgtctgtag gggcgtctcc gtgggcgccc tttcatccgg ttctgccaat taaagaatac
     3001 gtacctatca aattaagtga gggcgcccac ggagggacgc ctcttcaaat caggccatct
     3061 ttcagatgaa tttcaggcga tgtttagttg cgacgcaaaa taaaactttt tggtgtccag
     3121 ttagttaaca ttttggtaac atggcgcgag ttggcgcttt cccctgaagg cgctggtaag
     3181 cctcgaaccg acgccgccac aacgacaacg ccggtccagt atctgggagg aattgagatg
     3241 caacccagtt caaggacgct tcgtcctctt agctctcatc ttgcgagtgt ggtcctcgtc
     3301 agtttgtcct gcctcataat caattcgcac gctgaaacca g
//
LOCUS       DASSFP010000313         3953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1416416, whole genome shotgun sequence.
ACCESSION   DASSFP010000313 DASSFP010000000
VERSION     DASSFP010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416416"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFH91_06950"
     CDS             complement(<1..929)
                     /locus_tag="VFH91_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6862766.1"
                     /translation="MTEELKQVEPVFNGRDADIWPVLSDAQMTRIGAHGRHRKVSAGE
                     VLVREGDLTTHLFVITAGEVQLIRPSEDGPELIALYTRGKFTGEVNMLSGRRGFVTIQ
                     ARTDGEVIEVTREELLSLVQTDSDLSDILMRAFLLRRLELIAHGFGDVILVGSLFCAA
                     TLRIKEFLTRNNHPYSFVDLDEDAGTQELLDRFHIDEGDIPVLICRGQVVLRNPQNQE
                     IANVLGFNETIDPVHLRDLVIIGAGPSGLAAAVYGASEGLDVLVLESNAPGGQAGSSS
                     KIENYLGFPTGISGQELAAAAYAQAQKFGAQVL"
     gene            1222..2583
                     /locus_tag="VFH91_06955"
     CDS             1222..2583
                     /locus_tag="VFH91_06955"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET6862767.1"
                     /translation="MIKKLLVAAILCAVTTQLAKSQRVVQINEPDALNPAEVSIAINP
                     KNPDNMVAASFQSGRAPKPRAGSYNYVTMDGGQTWKTVPTADPKNLFQGDDVVAFSND
                     GIAYHAHLSFDGIRVSRPARAENGMIVNVSHDGGLTWTDGTAVVNHINTVTPFEDKPG
                     LAVDNAPTSRFAGNVYMAWTRFDVYGSTNPDHKSQIYFSRSTDKGQTFSMPIRISDVG
                     GDCADSDNTVEGAVPAVGPKGEVYVVWAGPLGLVFDKSTDGGLTFGKDKVLGNFPGGW
                     DFAVEGLDRANGMPVTGVDLSDGPNKGTLYVNWIDARNGDPDVFVMSSKDGGETWSAP
                     VRVNDDQIKNGKAQFFTWMSIDPGDGSLNLVFYDRRNTKGAKTEVVLARSVDGGRTFV
                     NHKIDLPEFEANSQVFFGDYTGISAFNGRVVPVFMHFVGKNKLAVSVALFQFKPGTQE
                     KVQ"
     gene            2870..3463
                     /locus_tag="VFH91_06960"
     CDS             2870..3463
                     /locus_tag="VFH91_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET6862768.1"
                     /translation="MKDEQVRELLYQALETELGGVQVYTTAIRCAENDDLKEEWEEYL
                     EQTKKHVQIVQDVLTKLGLDPNEQTPGRKIVKNIGETLVASMETALSDGKPGAAQLVA
                     CEAVVLAETKDHQNWHLIGEVVKNAKGELARVLKQAHEQVEDEEDEHLYHTKGWCREL
                     WIESLGMKAVLPPPEEVKQVETAIGAARAEQARSQMV"
     gene            complement(3518..>3953)
                     /locus_tag="VFH91_06965"
     CDS             complement(3518..>3953)
                     /locus_tag="VFH91_06965"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET6862769.1"
                     /translation="FRSYRRGACGSVMPVLALVSSGPWIKRTRDSYTSPMTDDFYCDE
                     VLSGRTAVQVVCETDGVLAYHHTKPYWPVHVVVIPKRHISSLLALQESDNLLLIEIVQ
                     VIKEIANRVVQERGACRVLTNLGQYQDSKHLHWHINFGEPVK"
BASE COUNT         1024 a          950 c          994 g          985 t
ORIGIN      
        1 atcagcacct gcgccccaaa cttttgtgct tgagcgtagg ctgcagcggc gagctcttgc
       61 ccggaaatgc cggtgggaaa gccaagatag ttctcaatct ttgaactcga accagcctgt
      121 ccgccgggtg cattggactc caggaccaga acatctaatc cttccgatgc tccataaacc
      181 gctgccgcta atcccgatgg accagcacca ataatcacca aatcgcgaag atgtaccgga
      241 tcgatcgttt cattaaaccc gagaacattc gcgatctctt gattctgagg atttcgcaac
      301 actacctgcc cacgacaaat caaaaccgga atgtcgcctt cgtcaatatg aaaccgatca
      361 agcaattcct gggtgcccgc gtcttcgtct agatcgacaa atgaataggg gtgattattt
      421 cgagttagga attctttgat tcggagagtt gcggcgcaga ataaggagcc aaccagaata
      481 acgtctccga agccgtgtgc gatcagctcg agccgtcgca gcagaaacgc tcgcatcaga
      541 atatcgctca ggtcgctgtc agtctgcaca agactcaaga gctcttctcg ggtaacctcg
      601 ataacctctc cgtctgtgcg agcctgaatt gtaacgaacc cacgtctacc ggatagcatg
      661 tttacttcac cggtaaattt tcccctggta tagagagcga tgagctctgg tccatcttcg
      721 gatgggcgga ttaattgaac ttcacccgcc gttattacga aaagatgcgt agtgagatcg
      781 ccctccctta ccaacacttc gccggcagag acctttcgat ggcgaccgtg cgcgccaatc
      841 ctggtcatct gagcgtcgct taatactggc cagatatcgg cgtcgcgccc attaaaaact
      901 ggttctactt gtttgagttc ttcagtcact tcttttgtcc ttggtcagtc ttcttggtca
      961 gtagtccgtt gtcagcagtc cgttcattgt ccgttgtccg tggttcgttg ttattcgtgg
     1021 tccgttgtta tgtgacaact gactacggac aactgacaac tgacaattct gaattcgatc
     1081 cattttatta aatctccgtt cagttggtcc tttctcttgc gctttgtgct ttgtagtttg
     1141 tactttgtgg gttggactcc aggatctaca tgaccctaat acgacctgta acagcacaaa
     1201 tcacaagtaa ccacatccac catgatcaaa aaacttttag tagcagcaat attgtgtgcc
     1261 gtaactactc aactggcaaa gtcacagagg gtagttcaga ttaacgagcc ggatgcgcta
     1321 aatcctgcag aagtgagcat cgcaattaat cctaagaatc ccgacaatat ggttgcggcg
     1381 tcatttcagt cggggcgagc gcccaaacct cgcgctggaa gttataacta cgtaacaatg
     1441 gacggcggcc aaacatggaa aaccgtaccg acagcggatc cgaagaatct gtttcagggt
     1501 gacgacgtag tcgcattcag caacgacggt atcgcgtatc atgcacacct ctcgttcgat
     1561 ggcatcagag tatcgcgacc agcacgcgcc gagaacggca tgatcgtaaa tgtgtcacat
     1621 gatggtggac ttacgtggac cgacggaacc gccgtagtaa atcacataaa cacagttaca
     1681 cctttcgaag ataaacccgg cctcgcggta gacaatgctc ccacctcacg ttttgccgga
     1741 aacgtgtata tggcatggac caggttcgat gtctatggca gtaccaatcc agatcacaaa
     1801 tcccaaatct atttctctcg atcaacagat aaaggtcaaa cgttttcaat gccgattcgg
     1861 atttccgatg ttggcggaga ttgtgcggat agtgacaaca cggtcgaagg agcagtacct
     1921 gctgttggtc caaaaggcga agtttatgtg gtttgggccg gtcctcttgg cctcgtgttc
     1981 gataaatcta ccgacggcgg tcttaccttt ggcaaagaca aagtcctggg aaactttccg
     2041 ggtggttggg atttcgcggt tgagggactg gatcgcgcca atggtatgcc tgtaaccgga
     2101 gttgatctaa gtgacgggcc aaacaaagga acgctttatg tgaattggat cgacgcacga
     2161 aatggcgatc ctgacgtttt tgtgatgtct tcaaaggatg gcggtgaaac gtggagtgct
     2221 ccagttcgcg tcaatgatga ccagattaaa aatggaaagg cacaattctt cacctggatg
     2281 tcgattgacc caggcgatgg ctctttgaac ctggtctttt atgatcgtcg caatacgaaa
     2341 ggagcgaaaa ctgaagtcgt tctggctcga agtgtcgacg gcggtcgaac atttgttaat
     2401 cacaaaatcg atctgcctga attcgaagcc aactctcaag tattctttgg agactatacg
     2461 ggaatctcag cctttaacgg tcgggtggta cccgtgttca tgcacttcgt tggtaagaat
     2521 aagctcgcgg tgtctgtcgc actctttcag ttcaaacccg gaacccagga gaaagttcaa
     2581 taaattccgc cccgtttcac ctgcctgaac ccttccgaac aacttcgcgc tgcgtgtttt
     2641 gagcattcag gagcaacgag agcatctggg cagaaggcgc caattcttaa ggctctttgg
     2701 tacgcctcac gtatttttat agaacacgcg tccgctaatc cgaaggagga tttcagccct
     2761 cggcgcaacg taaatgcaaa aatttaggga gtctgttcgc ggaatgcgaa tggcctgtct
     2821 attgcttaaa ggattaccgc acgctgaaaa tcttttcaag gaggggttta tgaaagatga
     2881 acaagtgcgg gagctcttgt atcaggcatt ggagactgag ttaggcggtg tacaggttta
     2941 tacgaccgcc attcggtgcg cagaaaacga tgacttgaaa gaagagtggg aagagtattt
     3001 ggaacaaaca aagaagcacg ttcagatcgt tcaggatgtg ttgactaagc ttggccttga
     3061 tcctaacgaa caaacgcctg gccgaaagat cgtaaagaat attggtgaga ctctagtcgc
     3121 gtcgatggag acggcactca gtgatggtaa acctggtgct gcccaacttg tcgcgtgtga
     3181 ggctgttgtt ttggctgaaa ccaaggatca tcaaaattgg catttaatcg gcgaggtcgt
     3241 aaaaaatgcg aagggtgaac ttgccagggt tttaaagcag gctcatgaac aagtcgaaga
     3301 tgaagaagac gaacaccttt atcacacaaa agggtggtgt cgggaactct ggattgagtc
     3361 attaggtatg aaggctgtgt tgcctcctcc cgaagaggtg aagcaagtag aaacggccat
     3421 cggggctgct cgcgccgaac aggctcgatc gcagatggtt tgaggtcgat ctgatcgctg
     3481 aagttaatag gacctatacg tctataggtc ctattcttca ttttacgggc tcaccaaaat
     3541 taatgtgcca gtgcaggtgc ttcgaatcct gatactggcc cagatttgtc aacactcgac
     3601 aagcgcctct ctcttggacc acccgatttg caatttcttt aataacctgg acgatttcaa
     3661 tcagtagaag attgtctgat tcctgaaggg ccaataaaga cgaaatatgc cgttttggaa
     3721 tgacaactac gtggaccggc cagtatggtt tcgtgtgatg atacgcgagc acaccgtcag
     3781 tttcgcagac aacctgcacc gctgttcggc cgctcaaaac ttcgtcgcaa tagaagtcgt
     3841 cagtcatggg tgaagtataa gagtcacggg ttcgtttaat ccagggacca ctagagacta
     3901 gcgcgagcac tggcattaca gaaccacaag cgccgcgcct gtacgacctg aat
//
LOCUS       DASSFP010000314         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_233029, whole genome shotgun sequence.
ACCESSION   DASSFP010000314 DASSFP010000000
VERSION     DASSFP010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233029"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFH91_06970"
     CDS             complement(<1..353)
                     /locus_tag="VFH91_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862770.1"
                     /translation="MKHYLACLLATAFLVASVTMPVAAQQAGFEEATLEKVKSKVAKL
                     GVGEKAKATVILKNGTKVKGYIGQAGNDDFVLRNRKTDSPTTIRYSDVSKVESNRGHS
                     TARNIAIGVGIGVGAL"
     gene            355..495
                     /locus_tag="VFH91_06975"
     CDS             355..495
                     /locus_tag="VFH91_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862771.1"
                     /translation="MVSSLYRRTVSKLQAAAEWLSLDHDYKSNKDANKQLKIVHANEI
                     EC"
     gene            complement(685..2964)
                     /locus_tag="VFH91_06980"
     CDS             complement(685..2964)
                     /locus_tag="VFH91_06980"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862772.1"
                     /translation="MKIFAVLILFASLTFPSIALPQSREPWRSVRTNNLFVIGNADAD
                     SLQQVAAWLEFFHSAFSRLTSRSSIDSSVPTTVIVFRDEASFTPFKPLYQGKPANVSG
                     YFQPGEDANYIVISLEGSGRDRFATAFHEYVHLHLRNTVPGAPLWLNEGLAEFYGALQ
                     FSGGEALIGAPIPAYLQLLRSQPMLPLGQLFSIGTNSPHYNERDKAGIFYGESWALVH
                     FLMLRDGLSHQDQFSRFLQSLSRGDDAERAFEDSFSMTLSNAEQQLRDYIRSGELKSQ
                     RLDIGDSQQSYSSFMAMQRSSLLDGEVSYYLGDLLSHMGRIEDAERYFLQAISQQPDF
                     IPPYAALGLLRIRQQRYDEAKKYLQKACSSQQSYLIHYLYAYVLSREGFGPNGEFTSY
                     TKDSVNTIRDQLQTAIKLNPQFAPAYHLLALIDFVTDERLDQAVLMAEKAHQLDPSRS
                     SFSMLLAQIYLRQSNTVAARQVLESLTRDSNENVRAQAEGLIARLKESSGNRVKLSQS
                     VLAEPVQPASRGIVTGATSTGAARDGQTIDQSGPMPGVDEVLTRYVQALGGADAIKSV
                     TSRVTKGTLDVIGASRNGSFEIYSLAPNKTLTILQTSSLGIIKMGFNGQSGWVQTPGR
                     IRQLKGAELASLQLDSDFHSPIRIRSNYSKANLLGKSKIGYREVYVLELQPAGGAAEK
                     LYLDAESYLPVRLNAIRINGAQLMPVEVYFDEWQKVDGINIPFTITESFPRLTLVFKA
                     TEIKHRVAIDGKLFDSPAR"
BASE COUNT          829 a          803 c          838 g          817 t
ORIGIN      
        1 agtgcaccca ctccgatgcc gacaccaatt gcaatgttcc gggcagttga gtgacctcta
       61 ttactctcaa cttttgaaac gtcgctgtag cgaatcgtcg ttggcgagtc agtttttcga
      121 ttgcgtaata cgaagtcatc attcccggcc tggccgatgt agcccttcac ttttgttcca
      181 ttctttagaa tcacagtagc ctttgccttc tcaccaaccc cgagtttggc aaccttgctc
      241 ttgacctttt ccaaagtcgc ttcctcaaag ccagcttgtt gagcagcaac gggcattgtg
      301 acggaggcga ctaaaaacgc agtagccagt aaacaggcca aatagtgttt catcatggtt
      361 tccagtctgt atcggcgcac cgtgagtaaa ttgcaagcag cagccgagtg gttgtcgtta
      421 gatcacgatt acaagagcaa caaggatgcc aataagcagt tgaagatcgt tcacgcaaat
      481 gagattgagt gttaaagcat gagagtcaaa atcgttctag tgcaatatca atccgacaac
      541 tggaccactc gcgtgtagat aaatttgact gggacaaaca tgtttatact tacttggcgt
      601 gacaattgag cttgctggat accgttcgaa cagtgagcga acctaaaaag gaactaacgc
      661 ctgatttcta acctcggcga tctcttatct tgccggggag tcgaagagtt tgccgtcaat
      721 tgcaactcta tgcttgattt cggtcgcttt aaagacgaga gtgagtctcg gaaaactttc
      781 agtgatcgta aacggaatgt tgatcccgtc tactttttgc cattcatcaa aataaacttc
      841 cacaggcatc aattgcgctc cattaatacg aatagcattg agccttactg gaaggtacga
      901 ttcggcatcc aggtagagct tctcggcggc tccccccgca ggttgcaact caagcacgta
      961 gacctcgcga tatccgatct tactttttcc aagcagattg gccttcgaat agttacttct
     1021 gattctgata ggtgaatgga aatcggaatc gagttgaagt gaagcgagtt ccgcgccctt
     1081 cagctgccgg attctacccg gcgtctgaac ccagccactc tgaccgttga agcccatctt
     1141 aatgatcccg agtgaacttg tctgcaatat ggttagagtc ttgtttggcg caagactata
     1201 aatctcaaaa ctaccattgc gactggctcc aataacatcc aacgtaccct tcgtcacacg
     1261 agaggttacc gacttaattg catcagcacc gcccagcgcc tgaacatatc gcgtgagtac
     1321 ttcatccacg cctggcatcg gtcctgattg atcgattgtt tgaccgtcgc gagctgcgcc
     1381 ggtagaggta gctcccgtca cgattccacg agaagcaggc tggaccggct cagcgaggac
     1441 ggattgactg agcttaactc gattacccga cgactccttt aaacgagcga ttagaccctc
     1501 tgcttgtgct ctcacgtttt cgtttgaatc gcgcgtgagg ctttccagaa cttgacgggc
     1561 agcgactgta ttcgactgtc tcaggtagat ttgggccagg agcatcgaga agctgcttct
     1621 tgacggatcg agttggtgcg ccttttctgc catcaagacg gcctggtcca gtcgttcatc
     1681 ggtcacaaaa tcgatcagag ccaacaggtg atacgcagga gcaaattgcg gattcagttt
     1741 gatcgccgtc tgtagctgat cgcggatggt attgacacta tcttttgtgt aagacgtgaa
     1801 ctcaccgttt ggtccaaagc cttcacgact tagaacgtac gcatataggt agtggattaa
     1861 atacgactgc tgggatgagc aggctttctg gaggtacttc ttagcctcat cgtaacgttg
     1921 ctggcgaatg cgaagcagcc cgagcgctgc atatggtggg atgaaatccg gctgctgcga
     1981 aatcgcctga agaaagtagc gttcagcatc ttcaatccgg cccatgtgcg agagcaaatc
     2041 accgaggtaa taactcactt cgccatcaag tagcgatgat cgctgcattg ccatgaaaga
     2101 gctgtagctt tgttggctgt cgccaatatc gagccgttga gatttaagtt ccccgctgcg
     2161 aatgtaatcg cggagttgtt gttcagcgtt gctaagagtc atagagaaag agtcttcaaa
     2221 tgccctctcg gcgtcatcgc cacgcgacaa agattgtaag aaccggctga attgatcttg
     2281 atgcgaaaga ccatctctca acattagaaa atggaccaga gcccaggatt cgccataaaa
     2341 gatcccggcc ttgtctcttt cgttgtaatg cggtgagttt gtgccaatcg agaaaagttg
     2401 acccaggggt aacatcggtt ggctgcggag gagttgtaga taggcaggta ttggtgcgcc
     2461 gattaacgcc tcgccccctg aaaattgaag cgcaccgtaa aactctgcaa gtccctcatt
     2521 gagccacaat ggcgctcccg ggacagtatt gcgaaggtga agatgaacgt actcatggaa
     2581 cgccgtggca aagcgatctc gtccacttcc ttcaagcgag atgacgatgt agtttgcatc
     2641 ctcgcccggt tggaagtacc cggaaacatt cgccggttta ccctggtaca gtggcttgaa
     2701 tggagtgaag gaggcttcgt cacgaaagac aatgaccgtt gtgggtaccg acgagtcaat
     2761 cgacgaacgc gaggtgaggc gtgaaaacgc gctatgaaag aattcgagcc acgccgcgac
     2821 ttgttgaaga ctatcggcgt ccgcatttcc gattacgaaa agattgtttg ttcgaacact
     2881 ccgccatggc tcacgagatt gcggcagagc aatagacgga aaggttagtg acgcaaataa
     2941 aattagaaca gcaaagattt tcataggcga gtagattgag tcgggaggag gctcagcagg
     3001 tcttgctgga ggctcggttg actccggtat tgacccattc gaggatactc caccatgggg
     3061 cgcggagacc agataaacct ttgtacaccc ggaagtcggt actggttcag tttgcgttcc
     3121 tttgcgttct ctgcgtgatc tccgcgtact ctgcgtctgg tcggagttcc ttattacagc
     3181 tagccaagac gcagagaacg cggagatcac gcagagaaac gccgagaaat gaaattgaat
     3241 cggaaaatcg cgcattcacc taatgaccgt gatcttcgta atcgggt
//
LOCUS       DASSFP010000315         7696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_525116, whole genome shotgun sequence.
ACCESSION   DASSFP010000315 DASSFP010000000
VERSION     DASSFP010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525116"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFH91_06985"
     CDS             complement(<1..757)
                     /locus_tag="VFH91_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862773.1"
                     /translation="MNSLRLLPRRILAFVIRDFSLFVSYRMQFFLRILSVLTVVTTLF
                     FISKIFSGFTDPKFSQWQDPLASWLTGLAVLNYFMTGFSSLANSIRQEQMQGTLESVL
                     LTPINLPTVIVASSAWDFLETTFFSFLYLFFGRVFFNVHYRGSFFLAVVFLLLTTLVL
                     ACLGILSASFAMVYKRGDPFGIFLGAGSALFSGVFFPTQLLRDYAGPKLGNISKLLPA
                     TYGLDGIRQVLLEGHNLSQVTQPLITLLIFLTVL"
     gene            complement(799..1851)
                     /locus_tag="VFH91_06990"
     CDS             complement(799..1851)
                     /locus_tag="VFH91_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862774.1"
                     /translation="MSAAQNQQAEFPIQERPAVAPAILLNDLSKTYPVAFKKVRALFR
                     RSAKTPVEALSHVSLSIKPGEIFGLIGRNGAGKTTLTKIIATLVQPTTGTVEVLGYDS
                     VRQDERVRRLIGLATAEERSFYWRLNPIQNLMFFARLYGMAGSSARRRINELVELLEL
                     QEVAQRRFGELSTGNKQRLAVARALLAKPPVLLLDEPTRSLDPLAAARMRDLIQSLSQ
                     QVPPVTILFTSHNLAEVETLCARVAVISRGRIRGVDSPANLRSLTQNTEKIRITIRGT
                     TALETRTVLLPLGIDHQVEEFGDRVIITLNRQPGDEALDWVLKTLHQNNAVVLDIETE
                     RATLLDLLTQYETEER"
     gene            complement(1861..2676)
                     /locus_tag="VFH91_06995"
     CDS             complement(1861..2676)
                     /locus_tag="VFH91_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862775.1"
                     /translation="MNRLTILGFIIVAILIIVAIFAPWIATYDVGTTDLSMRYLAPSA
                     AHWFGTDSTGRDIFSRVVFGARISLQVGLIVVFVSALIGTFTGAIAGYYGGWVDRIVS
                     GYVFNVFLAFPGLLLAIAMVAFLGAGLNKLILALCIIGWVGYARLIRGQVLKVREYDF
                     VQAARALGASDARILFIHILPNAIQPLIVQASLGMAGAVLSEASLSFLGLGVPPPAPS
                     WGVMIEEARDISTLQAAPHALIFPGIAIALSVLAFNFIGDGLREYLDPRQRSR"
     gene            complement(2704..3708)
                     /locus_tag="VFH91_07000"
     CDS             complement(2704..3708)
                     /locus_tag="VFH91_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862776.1"
                     /translation="MRTNLRYRLLGIVKRLLILILVAWTVVSLVTLLIELVPGDPAVA
                     VLGEQATPEQFEQFREKHGLDRPPFFFSFPRDTNGQRHFVWHGSNNRYTDYWRELLHG
                     DMGLSFRNDRPVRDLILERYPATIELAIVALIIAVLISIPLGIVAGTHRGLLIDNVAS
                     VVALLGISLPSFVIGPMLVYVFAVKLGWLAPSGRFDWSDIILPAFTLGAALSAILTRM
                     VRSSVIEELGEDYVRTARAKGLSERTVLYKHVLKNGLIPVVTILGLQLGVLLAGAIIT
                     EKIFGWPGLGLLLVEDGIGKRDYRVVQGCVLVISMTYIVANMLTDAVYRWLDPRIRVG
                     "
     gene            complement(3972..4685)
                     /gene="ubiE"
                     /locus_tag="VFH91_07005"
     CDS             complement(3972..4685)
                     /gene="ubiE"
                     /locus_tag="VFH91_07005"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET6862777.1"
                     /translation="MSQKTNAATEHAGRVREMFATIATRYDFLNHLLSGNVDRRWRRL
                     LAQRVAGKLAPSSVILDVACGTGDLSLELFETTKVRVVGTDFCGPMLEIAAAKVPEDI
                     KLVESDALKLPFREGSFDLATIAFGLRNLSSVDDGLVELHRILKPGGWVAVLEFSQPS
                     NPLLRHLFGFYFHKVLPLMGGLISGSRSAYTYLPKSVQRFPDQKQLASLMGRAGFTEV
                     GYENLTGGIAAMHFGRKGK"
     gene            complement(4742..6124)
                     /locus_tag="VFH91_07010"
     CDS             complement(4742..6124)
                     /locus_tag="VFH91_07010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6862778.1"
                     /translation="MPLKSLRPLAVLLALVFLASNGAAQTAPEPEQLLNGLRILFWEK
                     PGSPDVLVKLRIHSGAAFDLAGKSGEMALLGDLLFPDAATTDFFSEMGGKLDIAVNYD
                     STTITMVGKAAQFDQIVEVLRNGLLATQLTPEVINRIRDSRLKIIRETAISPSIVADR
                     AIASRLFGDFPYGRPASGTPADLARVERADLMLARDRFLNSNNATLAIAGGIQKARAL
                     RTLRQLLGPWRKSEQIVPTSFRQPLPADTRALILNGPGTSTEIRLAVRGVARSDADYY
                     ATAILAKLIQSRWQSLYPELANKPAFVRSETHVLPGILVMGAGLNAQSTPDALLTARK
                     ALESVASTIASPAEMDRARSEATSELSGPFAKPESLPDPWLDAETFRLKEAQNAVIAL
                     QAVTPADVHRAAGRLLTNPSISTVIVGDPLQLKPVLQGRISFEVLGEVALPNTAPKPL
                     VKPGTNAKPS"
     gene            6433..7041
                     /locus_tag="VFH91_07015"
     CDS             6433..7041
                     /locus_tag="VFH91_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6862779.1"
                     /translation="MPEKTTDELLLERSANGDQLAFLELYERHRQPIFRFTYRLVGSV
                     EVAEDVTHDCFLSLIRKPENFQAENGASLRTYLFAAARNLVFKHFRNQGREGTYDDLD
                     EEPVAPRRHEPLRHVLAQELVDTVREAIVSLPPLQREALVLFEYEGLTLGEIAVIADT
                     DIGAVKSRLYRARERLKVILGPYISKGEQLNSSREIVTLKEA"
     gene            7043..>7696
                     /locus_tag="VFH91_07020"
     CDS             7043..>7696
                     /locus_tag="VFH91_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862780.1"
                     /translation="MSVEPGEEFISDDELKSLLERWGAPGPSRVLDERIETSFKKEFS
                     GAAGLPESVLLPHSREEVVSMKFCSKCEEQFADKFSFCPVDGTPLSSVTEQIEEPSLT
                     VSRQESTPAVDTITAFPAKAERDETVHEPAVPVARAAAAAGSSALALREEYHLTIMSD
                     EGLLSRLTHEVRDVAHEYELTWPEFKRDPFGFTKRTFVGYSQMLRKFLSNRNVVLAMG
                     "
BASE COUNT         2013 a         1938 c         1982 g         1763 t
ORIGIN      
        1 gaagtacagt aagaaaaatc agtaaggtga ttagtggttg cgtcacctga ctcaaattat
       61 gaccttcgag taatacctga cgaataccgt cgagtccata tgtggccggc agcaacttcg
      121 aaatattgcc gagcttcggg ccagcgtaat cccgcagcaa ctgcgtggga aaaaagaccc
      181 ctgaaaaaag cgccgaacct gctccaagga atatgccgaa gggatcgcca cgtttataga
      241 ccattgcgaa actggcagac aggatgccga ggcacgccaa tactaatgtc gttagcaata
      301 gaaacactac ggccagaaag aaacttcccc gatagtggac gttaaagaag acacgaccaa
      361 aaaatagata aaggaacgaa aagaaggtgg tttccaggaa gtcccaagcc gaacttgcca
      421 caatcaccgt cggtagatta attggggtca gtaatacact ctcgagcgtt ccctgcatct
      481 gctcttgcct gattgagttg gcgagactcg aaaaacccgt catgaagtaa ttgagtaccg
      541 ccaggcctgt gagccagctt gcgagcggat cttgccattg agaaaacttc ggatccgtaa
      601 aaccggagaa aatcttcgag ataaaaaaca gggtggtgac aaccgtcagc acagacagaa
      661 tgcgcagaaa gaattgcatg cgatagctga caaaaagact gaagtctcgt atcacgaaag
      721 ccagaatcct tcgaggcaat aagcgtaatg agttcataga gtctataaga cgctgtcagt
      781 ggtccaatga caaagcaatc atctttcttc ggtttcgtat tgcgtaagaa gatcgagcag
      841 cgtagctctt tcagtctcga tatccaacac cactgcgttg ttttgatgaa gtgtctttaa
      901 tacccagtcc agagcctcgt cgccgggctg tcgatttaaa gtaatgatca cccgatcgcc
      961 gaactcctca acctgatggt caataccgag aggcaaaaga acggttcttg tctcgagcgc
     1021 agttgtgcct cgtattgtaa ttcgaatctt ttctgtgttc tgcgtaaggc tgcgaagatt
     1081 agcaggtgag tcgacgccgc ggatccgtcc acgactaatc acggcaactc gagcacacag
     1141 agtttcaacc tctgccaggt tatgagacgt aaagaggata gtcactggag ggacctgctg
     1201 cgacagtgat tgaatcaagt ctctcattcg cgccgctgcc agtggatcta gtgaccgcgt
     1261 aggttcatcc agcaacagga ccggtggctt cgccagtaaa gcacgagcga ccgcaagtct
     1321 ctgcttgttg ccagtagaca gctctccaaa tcgcctttgt gctacctcct gtaactcgag
     1381 tagctcaact agttcgttga tccgacgtct cgcactgctg ccagccattc cgtacaagcg
     1441 cgcaaaaaac atcaaatttt ggatcgggtt cagtcgccaa tagaaactcc gttcttcggc
     1501 cgtcgcgagc ccgatcaacc gcctgacccg ttcgtcttgt ctcacgctgt cgtagccaag
     1561 aacctcaaca gtaccggttg tcggctgtac cagagttgcg attatcttag taagtgtcgt
     1621 tttcccagcg ccgttacggc ctattaaccc aaaaatctct ccaggtttga tactcagcga
     1681 aacgtgcgag agtgcttcaa caggtgtttt ggcggatctg cgaaacagcg cccggacttt
     1741 cttaaaagcc acggggtaag tcttggataa gtcgttaagg agaatggcag gagcgactgc
     1801 aggccgttct tggatcggga attcagcttg ctgattttgc gcagcggaca ttcacagtcc
     1861 tcagcgagat cgttgtctgg ggtcgagata ctcacgcagc ccatcaccga tgaaattgaa
     1921 agccaacacg gacagtgcga tagcaatccc tggaaatata agcgcatgag gagcagcctg
     1981 tagcgttgaa atgtcacgtg cctcttcaat cattacaccc cacgaaggag cgggtggtgg
     2041 cactccaagc ccgaggaaag agagcgatgc ttcggaaaga acagccccag ccattccgag
     2101 actcgcctgg acaatcaaag gttgaattgc attagggagt atatgaatga agaggattcg
     2161 tgcatcactt gcgcccagtg ctcgggcggc ttgtacgaag tcatattctc gcacttttaa
     2221 cacctgacct cgaatcaatc tggcgtatcc cacccaaccg atgatgcaga gagcgagtat
     2281 caacttattc aaccccgcac cgaggaatgc gaccatcgcg atagccagca gcaagcccgg
     2341 aaacgcaaga aacacgttga atacatatcc agaaacgatc cggtccaccc accctccgta
     2401 gtagccggcg atcgcgcccg taaaagttcc aattagtgcc gacacgaaga cgacgattaa
     2461 gcccacctgt aatgaaatac gagcgccaaa gaccactcgt gagaagatgt cgcgtccggt
     2521 tgagtcggtg ccgaaccagt gcgccgctga tggagcgagg tagcgcatcg atagatcggt
     2581 ggtgccaacg tcataggttg cgatccacgg cgcaaagatc gcgactatga ttagtatggc
     2641 tactattata aaacccagta ttgttaaccg gttcacgttt gaatcaccgt tagataaaag
     2701 ttctcaccca actcgaattc gtggatcgag ccagcggtaa acagcatctg tcagcatgtt
     2761 ggcgacaatg taagtcatgc ttataactaa cacgcatcct tgaacgacgc ggtagtctct
     2821 tttcccgatg ccgtcttcaa ccagcagtaa tccgagtcct ggccagccaa agattttctc
     2881 tgtaatgatg gcgccggcta ataaaacgcc gagttgtaat ccaagaatcg taacaacagg
     2941 aatgagaccg tttttgagta catgcttata aaggacagtt cgttcgctca agcccttagc
     3001 tcgtgcagtt ctcacataat cttccccgag ttcttcaata actgaagagc gcaccattct
     3061 cgtcaagatc gcagacagtg cagcgccaag tgtgaaagca ggcaggataa tatcgctcca
     3121 gtcgaaccta ccagacggcg cgagccagcc gagcttgact gcgaagacgt agacaagcat
     3181 cggtccaatg acaaaactgg gtagactaat accaagcaga gcgacaacag aagcgacatt
     3241 gtctattaat agaccgcgat gagttccagc aacgattccg agcggtattg agattaaaac
     3301 tgcgatgatt agggccacta tcgcaagttc gatagtcgca gggtatcgct ctaagatcag
     3361 atctcggacc ggcctatcat tccggaatga aaggcccatg tcaccgtgaa gtagttcacg
     3421 ccaataatcg gtataacgat tatttgatcc atgccagaca aaatgtctct gtccgttggt
     3481 atcgcgcgga aatgagaaga agaagggtgg acggtccaat ccgtgctttt cgcggaactg
     3541 ttcgaactgt tccggagtcg cttgttcacc gagcacggcg actgcgggat cacctgggac
     3601 gagttcaatt agtagtgtta cgagggatac tactgtccag gcaaccagga tcagaatcag
     3661 caatcgtttg acgatgccga gtaatctgta acgaaggttg gttcgcactc gggcgatttt
     3721 ctcggagcgg tcgaagctta gaagtcggcg gaatcatagc acaagcagaa gaacgtttct
     3781 ttgcgttctc tctgcgtctt ggctgtgtag ctctaataag gaacttcgac aagacgccag
     3841 aaatagaaaa acgtagcccg gggcaggttc gaatactcgc acagccccga caaccgcgcg
     3901 cttaaacaaa ccaaaccccg tgactacgta tctggttgca aactgttata gcgctgcaac
     3961 caatccgaaa attatttccc tttgcggccg aaatgcatgg ccgcaattcc tcctgtcagg
     4021 ttctcgtaac ccacctctgt aaaccccgct ctgcccatta aagaagcaag ctgtttttga
     4081 tccggaaagc gctgcaccga tttcggtagg tatgtatatg cgcttcggga tccacttatc
     4141 agtccaccca tcagcggcaa caccttatgg aaatagaaac caaagagatg tcgcagcaag
     4201 ggattggaag gttgtgaaaa ctcaagtact gcgacccaac cgccaggttt caaaatccgg
     4261 tggagttcga ctagaccatc gtcgacactc gacaagtttc gcaagccaaa agctatcgtc
     4321 gccagatcga aagacccttc acgaaagggc agcttcaatg catcactttc gactagcttg
     4381 atatcctctg gcacctttgc ggcagctatc tcgagcatgg gcccgcagaa gtctgttcca
     4441 acaactcgta ccttcgtagt ttcaaacagc tcgagtgata aatcacccgt tccgcacgcg
     4501 acgtccaaaa tcactgaact cggtgctagc tttcccgcga ctcgctgagc aaggagtcgt
     4561 cgccaccggc ggtccacatt tcccgacagc agatgattta aaaaatcata ccgagtcgca
     4621 atggtcgcaa acatttcgcg aactcggccc gcatgctcag ttgcggcgtt cgttttctgg
     4681 gacacagtta aaccaaattt gatgggggtt tggttgcaag acgcttgcaa acaatttgtg
     4741 attagctggg cttggcatta gtcccaggct taacaagggg tttcggagcg gtattaggaa
     4801 gcgcaacttc gccgagtact tcaaacgaaa ttcgtccctg aagcactggt tttagctgca
     4861 acgggtctcc cacaataacg gttgaaattg aaggattagt gagcagtcga ccagcggctc
     4921 gatgaacatc cgctggagtg acagcctgta aggcgatgac agcattctgc gcctctttta
     4981 gacgaaaagt ttcagcatcc aaccagggat caggcaaaga ttcgggcttt gcgaaagggc
     5041 cagacagctc agaggttgct tcggaccgcg cccgatccat ttcagcgggg ctggcaattg
     5101 tcgaagccac cgattcaaga gcctttcgag cggttaatag cgcatcggga gtggattgcg
     5161 cattcaaacc agcgcccatc acgaggattc caggtaacac atgcgtttca ctccgaacga
     5221 aagcaggctt attcgccagt tccggataaa gtgattgcca gcgactttga atcagttttg
     5281 ccagaattgc agtcgcataa taatcggcat ctgatcttgc gacgccccta acagctagtc
     5341 gtatttcagt acttgttccg ggaccattca aaatcagagc gcgagtatca gcaggtaaag
     5401 gctgtcgaaa cgacgttggc actatttgct cgcttttgcg ccatggacca agcagctgtc
     5461 gtaatgttcg cagcgcccgg gctttctgga taccgccggc gatcgccagg gttgcgttgt
     5521 tagagttaag gaatcgatcc cgggccagca taaggtcggc gcgctccact cgcgctagat
     5581 ctgcgggcgt ccctgatgct ggccgaccat aaggaaagtc gccgaataag cgcgaagcaa
     5641 ttgccctatc agcaacaatt gaaggggata tggctgtttc gcgtatgatt tttaagcgcg
     5701 aatcccgtat tcgattaata acttcgggtg tgagctgggt cgccagcaat ccattacgga
     5761 gcacttctac aatctggtcg aattgcgcgg cttttcccac catagttatt gtggtcgaat
     5821 cgtagttaac tgcaatatcg agcttgcccc ccatctcgga gaagaaatct gttgtggcag
     5881 cgtctggaaa tagcaggtct ccgagcaaag ccatctcgcc ggacttacca gccaggtcaa
     5941 aggccgcacc actgtggatt cgaagcttga ccaacacgtc ggggctaccc ggcttttccc
     6001 aaaagagaat ccgaagccca tttagcaatt gctccggttc gggcgctgtt tgagcggccc
     6061 cgttcgaggc gaggaatact agagccaaca ggacggcgag cggccgcagg cttttcaaag
     6121 gcattggtag tatcaacatg tagaggcgaa tggcttttgg ctcgagcaaa actgtagtaa
     6181 agggtcgaaa acatcttaga tgccatgtgt ttgcccgtca aaccgtttag cttccaacgg
     6241 catccaaggc cttgattagc gagccgattg agaacaggta gcaagttttt cggcaaaatt
     6301 ctgtatgtac ttgctacctc cacgggatta gaattcaact cggtacgagc gatttctggc
     6361 tccggccgta acttttagct tgttggcggt aattattagt gggaagcaat tcctgcagga
     6421 tcgagtgctt gattgcccga gaagaccaca gacgagcttt tactggaaag atcggcgaac
     6481 ggtgaccagc tagcctttct ggaattatac gaacgtcatc gtcaaccaat tttcagattt
     6541 acgtaccggc tggtcggttc ggtcgaggta gccgaggacg taacgcacga ttgctttctg
     6601 agcctgatcc gtaaaccgga aaattttcaa gcggagaatg gtgcgtcttt gagaacctat
     6661 ctatttgcgg ccgctcggaa tcttgtattc aagcatttcc ggaatcaggg tcgcgagggt
     6721 acctacgacg acttggatga agagccggtg gcgccccggc gacacgagcc acttcggcac
     6781 gttttagctc aggagttggt tgatacggtg cgcgaggcga ttgtaagttt gccgccgctc
     6841 cagagagagg ctttagtgct gttcgaatat gaaggattaa ctctcggtga gattgctgtg
     6901 attgccgaca ccgacatcgg cgcagtcaag tcacggctgt atcgagccag agaacgattg
     6961 aaagtcatac ttggacctta tatttctaaa ggcgagcaat tgaatagtag tcgggaaatt
     7021 gttactttga aggaggcctg aaatgagcgt tgagccgggt gaagagttta tttcggatga
     7081 tgagctcaag tcgctgctcg aacgctgggg cgcgccagga ccttcgagag ttctcgacga
     7141 gcgcattgaa acgagcttta agaaagaatt ttcaggtgca gctggactgc cagagtctgt
     7201 gctgctcccc catagtcgag aagaggtagt ttcgatgaag ttttgttcca agtgcgaaga
     7261 acagtttgca gacaagttca gcttttgccc tgttgacggt actcctttat ccagcgtcac
     7321 agagcaaata gaagaaccga gtctcacggt cagcaggcag gaatcaacac cagctgttga
     7381 caccatcact gcatttccgg ctaaggctga gagagacgaa accgtgcatg agcccgctgt
     7441 ccctgttgcc agggccgctg ctgccgccgg gtcgtcagcc ctggcgttga gagaagaata
     7501 tcatctgacg atcatgagtg atgaggggct cctttctcgt ttaactcatg aagttcgtga
     7561 cgtggcccat gaatacgaat taacgtggcc cgagttcaag cgcgatccat ttggcttcac
     7621 gaagcgcacc ttcgtcggtt acagccaaat gctacgcaaa tttctttcga accgtaatgt
     7681 cgtgctcgcg atgggc
//
LOCUS       DASSFP010000316         5194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1278401, whole genome shotgun sequence.
ACCESSION   DASSFP010000316 DASSFP010000000
VERSION     DASSFP010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278401"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1523
                     /locus_tag="VFH91_07025"
     CDS             297..1523
                     /locus_tag="VFH91_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6862781.1"
                     /translation="MNFFSRSTSALFLATSLLASSIVAVAQTSTNSTDQTSKAGTTQA
                     SSTTQTDAKSAEAKKPQTAAATPATPKSNKPLATNEDPAMIGKRNINTGLIAKMSGST
                     EKEVRQGREAAAEVDRQAKFIDDPIITEYVNRVGQNIVLHSDAKVPFTIKVIDSDEVN
                     AFALPGGFFYVNKGLLLAADNEAELAGVMAHEIAHVAARHAVENQTKATLLEYAAIGG
                     SIFLGGIPGMIYQNTAGIGLLGIFMKFSRSAEEEADKLGVQYMYAAGYDPSAMATMFE
                     KLEAKNKKKPGFIARAFATHPAPPDRRASALALAARFPEHEEYVISSSEFQRVKGRLL
                     KLSNARAAATGTIQGSDNGNAPDRPTLKRRSPTPDDTTTTTTDGEQKPAETQQGPPKL
                     KRNDGTPTPTKTPDKP"
     gene            1901..2356
                     /gene="rplM"
                     /locus_tag="VFH91_07030"
     CDS             1901..2356
                     /gene="rplM"
                     /locus_tag="VFH91_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET6862782.1"
                     /translation="MSTYFPSGKDLAGKRKWYVIDASGLTVGHLASEVASILSGKRNP
                     EWTPFMDVGDHVVVINARKAVLKGNKVDQKLYRHHTRYPGGLREVSAREMIATKPEKV
                     LELAIKGMLPKTKLGKAMAKKLKVYADADHPHSAQRPQTRELAHLTNSK"
     gene            2384..2776
                     /gene="rpsI"
                     /locus_tag="VFH91_07035"
     CDS             2384..2776
                     /gene="rpsI"
                     /locus_tag="VFH91_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET6862783.1"
                     /translation="MADIQYYGTGRRKTSTARVYLRPGAGEVQVNRKAFDRYFPNETL
                     RMIIRQPLQLTETTNKFDLYINVSGGGPAGQAGAIRHGITRALLEFNADLRPTLKHAG
                     LITRDPRIKERKKYGQKGARKRFQFSKR"
     gene            2902..3813
                     /gene="rpsB"
                     /locus_tag="VFH91_07040"
     CDS             2902..3813
                     /gene="rpsB"
                     /locus_tag="VFH91_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET6862784.1"
                     /translation="MVSVTMKELLEAGVHFGHQVRRWNPKMKEFIFGERNGIYIIDLQ
                     KTQRMFREAISFVTNLIAEDKGKTVLFVGTKRQAQDAIREESEKCSQYYVNQRWLGGL
                     LTNFQTVQKSIKRLKDLEAMQTDGRYEKLTKKERIKLDRERESLNKNLSGIKSMTRLP
                     DAIFIIDVKKEEIAVAEANRLGIPIVAVVDTNCSPEGIDYVIPGNDDALRAVRLFASR
                     ISDAILEGHQIATEGGVVAAEAGESAEPSSDIQAAPVPAPAETSQENGDKSGDDAQTE
                     LTTSQVGETAQAGTVSDESTESVVAAS"
     gene            3922..4584
                     /gene="tsf"
                     /locus_tag="VFH91_07045"
     CDS             3922..4584
                     /gene="tsf"
                     /locus_tag="VFH91_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET6862785.1"
                     /translation="MAEITATAVKQLREKTGAGMMECKNALVEAQGNEEKAVDILRER
                     GLASAKKREGRIAAEGIVGSYIHMGGKVGVLVELNCETDFVARGEEFQQLVKDIAMHV
                     AAAEPRYVSREEVPEQVLDKEREIARAQAMNDPKNANKPDQVIEKIVEGRLNKFYEET
                     VLNDQPFVKDPAKTVAELVTEKVSKTGERITVRRFTRYKMGEGLDRRSEDFGGDVASL
                     LS"
     gene            4601..>5194
                     /gene="pyrH"
                     /locus_tag="VFH91_07050"
     CDS             4601..>5194
                     /gene="pyrH"
                     /locus_tag="VFH91_07050"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET6862786.1"
                     /translation="MTIPKAPKADLVYRRVLLKISGEALMGEQSYGIDVNVARSVAEE
                     LKAVHELGVQVAVVVGGGNIFRGVSKSAGNMDRSSADYIGMLATVMNAVVLQDALEKI
                     SVDTRVLSAVDIPQLAESFIRRRAVRHLEKGRVVIFAAGTGNPYFTTDSAAALRALEI
                     KADIILKATKVDGVYSADPMKDSQAEFFNEITYQQVLE"
BASE COUNT         1389 a         1309 c         1346 g         1150 t
ORIGIN      
        1 tcccggtttc tgtttactac taactcaggt acagctatag ttacgacact gatgtcaggt
       61 tcgcacaggc aggctacgaa acccttcccg actaaagtag ttaaggcgct tcgcgcaacc
      121 agtcaacgag ccgttgcatc atagactgcg cccctttaat aagcgaaaac cgacatcata
      181 aacacctaac ccctcaggtc acgtcgattc tcggtagttt ttgggtctgc aacgctttac
      241 acaaatcaac gttcattcgg tccggccatg tttgaagtta tctggaggtt ttaataatga
      301 acttcttctc ccgctctacc agcgccttat ttttagccac ttcgctcctg gccagctcca
      361 ttgtagcggt tgcgcagaca agcactaatt ctacagatca gacttcaaag gccggtacga
      421 ctcaggcgag cagcacaact caaactgacg cgaaatcggc cgaggcgaaa aagccacaga
      481 ccgctgccgc taccccggct acgccaaaat caaacaagcc tcttgccact aacgaagatc
      541 cagcgatgat cggaaagagg aatataaata ccggcctaat cgccaaaatg agcggatcca
      601 ctgaaaagga agtccggcag ggtcgggaag cagctgcgga agttgatcgt caggcgaagt
      661 ttattgatga cccaatcatt acggagtacg taaaccgtgt cgggcaaaac atcgtgctgc
      721 actccgacgc gaaagtgccc tttacgatta aggtcattga ttcggatgag gttaacgctt
      781 ttgccttgcc cggtggcttt ttctacgtaa acaagggtct tttgctcgct gctgacaacg
      841 aagctgagtt agctggcgta atggctcatg aaattgcaca cgtagcagcc aggcacgccg
      901 ttgaaaacca gacgaaagcc acattactgg aatatgcagc aattggcggc tcgattttcc
      961 ttggtggcat cccgggaatg atttaccaga atacggccgg tattggactg ctcggcattt
     1021 tcatgaagtt cagccgcagt gctgaagagg aagccgataa gcttggtgtc cagtacatgt
     1081 acgccgccgg atatgatcca agcgcaatgg ccacaatgtt tgagaagctc gaagctaaga
     1141 acaagaagaa accgggattc attgctcgtg cattcgctac ccatccggct ccacccgatc
     1201 gtcgggcatc cgcattggcc ctcgcagccc gttttcctga gcatgaagag tatgtaatca
     1261 gcagttccga gtttcagcgc gtcaaaggcc gattgctgaa actgtccaac gctcgggccg
     1321 ctgcaaccgg cactatacag ggaagtgaca atggtaatgc gccggatcga ccgacattga
     1381 agaggcgttc accgactcct gacgacacca cgactactac gaccgatggt gagcagaagc
     1441 cggctgaaac acagcagggt ccgccaaaac tcaagagaaa cgatggtact cctacgccga
     1501 cgaaaacccc tgacaagcca tagttccggt taaaatttag aacaaaaggc cctcggctta
     1561 atgccggggg ctttttttcg aacccaatta tttgagaatt atgaattgag atttgataat
     1621 ttcctggctc cggcccgttg cgtcggcgtg ggactgatcg gcccagagcg ttacctaacc
     1681 tggcttactc tgcgcaacca ctctgtgaaa cctttgtgtc ccctgagcct ctgtggtggt
     1741 ggttttctta gcactctgct cacccacaga ggcgcagagg acacaaaggt tgcacagaga
     1801 ttaggactcc agctttagcc tgctcagctt gacaattttt tgagggcttc ggtattctaa
     1861 ccgttcgcgt tttattcgct gaaaacagag atagttaact atgtcgactt actttcccag
     1921 cggtaaagac cttgctggaa aacgcaaatg gtacgttata gacgccagtg gcctcacagt
     1981 tggtcatctc gcgtctgaag ttgcttcaat tttgagtggc aagcgaaatc cggagtggac
     2041 tccattcatg gatgttggcg accacgtggt cgtaatcaac gcgcgaaagg ccgttttaaa
     2101 gggaaacaag gtggaccaga aactctaccg gcatcacacc cgttatcccg ggggcctgcg
     2161 ggaagtatca gcgcgagaga tgattgcaac caagccggag aaagtcctgg aacttgcgat
     2221 taagggcatg ctgccgaaga ctaaactcgg caaagcgatg gcgaagaagc ttaaggttta
     2281 tgccgatgcc gaccatccac attccgccca gcgcccacaa actcgcgaac tcgctcacct
     2341 gacaaactca aagtaacaat tcaaaaagat aagaggaaag acagtggctg atattcagta
     2401 ttacggaacc gggcggcgca aaacatcgac tgccagagtt tacctgcgac ccggtgctgg
     2461 agaagttcag gtcaatcgca aagcgttcga tcgttacttt ccgaacgaga cgctgcgaat
     2521 gattattcgt cagccgttgc aactcactga aacgactaat aagttcgatc tttatatcaa
     2581 tgtctccggc ggtggacctg ccggtcaggc aggcgccatc cgtcacggca ttacacgcgc
     2641 actgctcgag ttcaatgctg acttgaggcc aacactcaaa catgctggcc tgatcacacg
     2701 cgatccacga atcaaggaac gcaaaaagta cggacagaag ggtgctcgga aacgcttcca
     2761 gttttcgaaa cggtaagtcc cgatttcgag ttttgtttcg aaatctgaat ccattgtcgc
     2821 ggcacgattg gttgctttct gctgtgaaag tccagagacc gcggcgagta catcaaacca
     2881 attaacaaag gagaaagtag tttggtttcg gtaacgatga aagaattgct ggaggccggt
     2941 gttcactttg gccaccaggt aagacgttgg aatccaaaga tgaaggaatt tatctttggc
     3001 gaacgtaatg gcatctacat tattgacctg cagaagacgc agcgaatgtt tcgcgaggca
     3061 atcagcttcg taaccaacct cattgctgaa gataaaggta agactgtgct gtttgtcggc
     3121 acaaaaagac aggctcagga cgcgatccgc gaagagtctg aaaagtgcag ccagtattat
     3181 gtaaaccaac gatggctcgg tggtttgctt actaacttcc aaacagtcca aaagtcaatt
     3241 aagcgtctta aggatctcga agcgatgcaa actgatggtc gttacgagaa gttgaccaaa
     3301 aaggaacgca tcaaactcga ccgcgaacgg gaaagtctca acaagaacct ttccggcatt
     3361 aagtcgatga ccagattgcc tgacgcgatc tttatcatcg acgtcaaaaa agaagagatt
     3421 gcggtagccg aagctaaccg tctgggcatt ccaatcgtcg ccgtcgttga cacaaattgt
     3481 tcgcctgaag gaatcgatta cgtcatcccc ggtaacgacg acgccttgcg agcagtacga
     3541 ctcttcgcct cgcgtatttc cgacgcaatc cttgaagggc atcagatcgc aactgagggc
     3601 ggcgttgtgg cggccgaagc tggcgaaagc gcagaaccaa gctctgacat acaagctgct
     3661 cctgttcctg ccccagcgga aaccagccag gaaaatggcg ataagagcgg agacgacgcg
     3721 cagaccgaac taacgacttc acaggtcggc gaaaccgcac aggctggaac ggtgtccgac
     3781 gaaagcaccg aatccgtagt agcggctagc taaacgatat tcttactctc ggcgagcgca
     3841 gcctttaagg cggactatcc gccgggctgc gtttttcatt gagcgaatca aattaaataa
     3901 ttctcggaga gatttcgaac tatggcggaa ataacagcaa cagcagtcaa acagcttcgc
     3961 gagaaaaccg gcgccggcat gatggaatgc aaaaatgcac tcgtcgaagc tcaaggtaat
     4021 gaagaaaagg cagtagacat tctgcgtgag cgtggtcttg cctcagctaa gaagcgcgag
     4081 ggaagaattg ccgcggaagg cattgtcggc tcttacattc acatgggcgg aaaagttgga
     4141 gtgctcgtcg agttaaactg cgagaccgac tttgtcgcgc gtggcgaaga gttccaacaa
     4201 ctcgttaagg acatcgcgat gcatgtagct gcggccgagc ctcgctatgt ctcacgcgaa
     4261 gaagttccgg agcaggtgct cgacaaagag cgagaaatcg cacgcgccca ggctatgaac
     4321 gatcccaaaa atgccaataa gcctgaccag gttatcgaga agattgttga aggccgtctc
     4381 aacaagttct atgaagagac tgtccttaac gaccaaccat tcgtgaagga tccggccaag
     4441 actgtcgccg agctcgtcac agaaaaggtc tcgaaaactg gtgagcgaat caccgtgcgt
     4501 cgcttcactc gctacaagat gggcgaaggt ttggaccgtc gcagtgaaga ctttggcggt
     4561 gacgtcgctt cccttttaag ctaggtcaga tccattaatc atgactattc ctaaagctcc
     4621 caaggccgat ctggtttatc gcagggtttt gctcaaaatt tcgggtgaag ccctgatggg
     4681 tgaacaaagc tacggcatcg acgtcaatgt cgctcggagt gtcgccgaag agctgaaagc
     4741 agttcacgag ttgggtgtgc aagtagccgt tgtcgtgggc gggggaaata tttttcgagg
     4801 agtttccaag agtgctggaa atatggaccg ctcctccgct gattacattg gcatgctcgc
     4861 aactgtcatg aatgccgtcg tgcttcaaga cgcactcgaa aaaatctctg tcgatacgcg
     4921 tgtgctgtca gcggtggata tcccacaatt ggctgaatca tttattcggc gccgggcagt
     4981 gcgccatctt gagaagggcc gcgtggtaat ctttgccgcc ggcacgggca atccttactt
     5041 caccacagat tcggctgcag ctcttcgcgc gcttgaaatc aaggccgaca taattctgaa
     5101 ggccacgaag gtcgatgggg tttattcggc tgatccaatg aaagactctc aggcagagtt
     5161 ctttaatgag attacttacc agcaggtttt ggag
//
LOCUS       DASSFP010000317         5902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_525442, whole genome shotgun sequence.
ACCESSION   DASSFP010000317 DASSFP010000000
VERSION     DASSFP010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525442"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFH91_07055"
     CDS             <1..623
                     /locus_tag="VFH91_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862787.1"
                     /translation="TLFGTGKPGQADGASPSFYEPGGLSVAGNKVYVADTNNHAIRVI
                     DLKTRQTSTLRINGLMPPVQSVASSSSDSAPNAETLKAPAQSLRPGTNAILSIDVALP
                     DGYHLNPAAPQRYKISVDTEKSIKIDAKNAQHSAKSLSLPLRIPISASEPGSAKVRAQ
                     VTLFYCREDNTGTCRIKTLVWEVPVEVSNNPGAGTEIKLKGKLSVD"
     gene            688..1698
                     /locus_tag="VFH91_07060"
     CDS             688..1698
                     /locus_tag="VFH91_07060"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET6862788.1"
                     /translation="MRIIAILFLLLLVCNISPAQQPQPKPPAPVRPVHTFSIVARDPA
                     TGELGVAVQSHWFSVGSIVAWAEAGVGAVATQSFVDPSYGRNGLDLMRAGKSGPDSLK
                     ELLAKDEAREVRQVAMIDAQGKVDAWTGKNDIQAAGHIVGTNFSVQANLMLNEKVWPA
                     MAHAFENTKGDLATRMLAALDAAQAAGGDIRGRQSAALIVVTGKPTGLPWKDRTFDLR
                     VDDSPEPLQELRRLVTLQRAYNHMNAGDLAVEKKDNEGALREYSAAEKLVPGNAEMIY
                     WHAVALVNMGRVDESLPLFRQVFAMDRNWITLTPRLPKAGLLPDDAAIINRIVGVAPA
                     RR"
     gene            1800..2084
                     /locus_tag="VFH91_07065"
     CDS             1800..2084
                     /locus_tag="VFH91_07065"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET6862789.1"
                     /translation="MADIIKCTHCGEKRPAIGFAPFPNELGQRIAKEICQPCWSGWLQ
                     KQTQIINHYGLDLTNPDAQTFLFDNIKGFLFGEVPNLAQIDTSKEGSVKW"
     gene            2270..2785
                     /locus_tag="VFH91_07070"
     CDS             2270..2785
                     /locus_tag="VFH91_07070"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6862790.1"
                     /translation="MKIQVTGLIASAAMTLALSGCQPSTNENINSNANASLARNTNTP
                     LPSPSPTVSETSRRAPTREEYERDKERYQREAKGAGSKVGTGLNDGWLWVKTRFDLAA
                     ADDLRDSTVNVDVDNGVVTLNGSVATVAQKTKAEAVAKSVEGVKSVKNMLKVVAANSN
                     ANTHASPTPRK"
     gene            3016..4680
                     /locus_tag="VFH91_07075"
     CDS             3016..4680
                     /locus_tag="VFH91_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6862791.1"
                     /translation="MPKVRLRLFVAISALIFVVIHSFSSTKAQRSGVDVYAITNARIV
                     TVSGPAIDKGTIVVRNGLIAAVGSNVTAPPDARVIDATGLTVYPGLIDSYTNLALPEP
                     APTPSPTGGGGAAFFLAAPRPSPGGPNSTQPAGLQPEVMVEDVIRTGASDLEASRNAG
                     ITSALTLPRTGIWLGQSAFINLAGDTPQQMIVRSPVAMHVGFTPLRGTYPGSLMGVFA
                     TLRQMMLDAQRYRDSRQIYERNPNGIRRPEVDRSLAALVPVVDGKMPVVMYANTEREI
                     DRALDLTSEFQLKTIIAGGRESGRLAGRLAQMKVPVLLSLNLPRRTTAALPEADPEPL
                     RVLRERVEAQQTAGKLVKAGVRFGFQSGSMTNMADLLSNAGKTIEAGLLSDDAVRAFT
                     IWPAEILGVSNQLGSIEQGKIANFTITRGDLFDRNSRIAHVFIDGREIDLRPSTGGPT
                     TRSTLTGTWTVTVNLGTDTKTITLNLQQEGERLRGSIEGPLGSAEIANASTMPELRFT
                     VPVNIEGQTKEATFVSTSSGNELRGTVTVEGGTPGTFTATRNSQPN"
     gene            4724..>5902
                     /locus_tag="VFH91_07080"
     CDS             4724..>5902
                     /locus_tag="VFH91_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6862792.1"
                     /translation="MKILRGFLIVIITMVAASYYDGDVFSQNPSETLIRNATVLTVTR
                     GTLQNTDVLLRNGKIAAVGKNLNASSNARIIDATGKFVMPGIIDCHSHSMLDTINEGT
                     LAVTSMVRTRDVLNVTDVDLYRELAGGVTTLNLLHGSANPIGGLNTVVKIKYGRPAEE
                     FIFPGAMPGIKFALGENVKRSSTPNQPGITRRYPNTRMGVEETIRDAFTRARDYKRAW
                     DEYRAAVAKGDKNILPPRRDLQLEPLVEILEGKRYVHAHCYVASEILMLINLADEFGF
                     KIRTFQHVLEGYKVAKEIAQHGAGASTFADSWAYKIEAYDAIAYNTAIMTRAGIVVSV
                     NSDSDERARRLNIDAAKAMHFGDLTEEQALKLITLNPAIQLGIQARVGSIEVGKDADV
                     A"
BASE COUNT         1459 a         1529 c         1522 g         1392 t
ORIGIN      
        1 aaaccttatt tggaacaggc aagccgggac aggcggatgg ggccagtccg tcattttatg
       61 agccaggagg cctttcggtt gcaggtaata aagtttacgt tgctgacacg aacaatcacg
      121 ccattcgtgt gattgatttg aaaaccaggc agacctcgac gctccgtatc aatggcttga
      181 tgccacccgt tcagtcagtc gcgtcttcat cgagcgatag tgctccgaac gcagaaacac
      241 taaaagctcc agctcagagt ttgcgtccgg gcactaatgc tatcctgtcg atcgatgtcg
      301 ccttgccgga tgggtatcat ctcaatcccg ccgcgccgca gcgttacaaa atttcagtcg
      361 acactgaaaa gtcgatcaaa attgacgcga agaatgctca gcattcagca aagtctctgt
      421 cgcttccttt gcgcatacca attagcgcat cagaaccggg ctcggcaaag gtccgggctc
      481 aagtcacact tttctattgt cgcgaggaca atacaggaac ttgccgtatt aaaactcttg
      541 tttgggaagt ccctgtcgaa gttagtaaca acccaggcgc cggaacagag atcaagctga
      601 agggtaaact gtctgttgac tgattcgtta tgctttgcac aaagagtcgg attcgttacc
      661 cactgtcaaa ggagctcact atttgctatg cgtataatcg ccatcctttt cctcctgctg
      721 ttagtttgca acatctcacc agctcaacaa cctcagccaa agccgcccgc gccagttcga
      781 ccagtgcaca ctttttccat agtcgcccgc gatccggcga ccggtgagct gggagtcgcc
      841 gtgcaatcgc actggttttc tgttggttca atcgtcgcct gggccgaggc cggcgtcggg
      901 gcagtcgcaa cacaatcatt cgtcgatccg agttatggcc gaaacggtct cgacttaatg
      961 cgcgccggta agagtggtcc agatagtctg aaggaactac tggcaaagga cgaagctcgt
     1021 gaggtgcggc aagtcgcaat gatcgacgct caaggtaaag tcgacgcgtg gaccggcaag
     1081 aatgatattc aagccgccgg ccatattgtc ggaaccaact tttcggtgca ggccaatctg
     1141 atgctgaacg aaaaagtttg gcccgcaatg gctcatgcgt tcgagaatac gaagggcgat
     1201 ctggcaactc gaatgttggc tgcgttggat gctgcacaag cggccggcgg cgatatacgt
     1261 ggccgtcagt ccgcagcgct aattgtagtg acaggaaagc caactggtct tccctggaaa
     1321 gatcgaacct tcgatctgcg agttgacgat agtccggaac cgttgcagga attaagacgt
     1381 ctcgtcacgt tgcagcgcgc ttacaatcac atgaacgcgg gagatctggc ggtcgaaaag
     1441 aaggataacg aaggcgcgtt acgtgagtat tcagcggctg aaaaactggt gcccggaaat
     1501 gctgagatga tctactggca tgctgtagct ctcgtaaaca tggggcgagt agatgaatct
     1561 ttgccgttgt ttcgccaggt gtttgcgatg gaccgcaact ggatcacgct taccccacgt
     1621 ttgccaaaag caggattgct gccggacgat gcagcgataa ttaatcgtat cgtcggcgtt
     1681 gctccggctc gccgctgagc cccattaatt agaatctgaa cctctctgcc aagttaggac
     1741 actacgcggt ggtcacatgt tgactataga gcttataatt tcgtcacaag gagaaaatga
     1801 tggccgacat aattaagtgc acgcattgtg gcgaaaaacg tcccgccata ggctttgcgc
     1861 cttttcccaa cgagcttgga caacgcattg caaaagaaat ttgccagccc tgttggagtg
     1921 gttggcttca aaaacagact cagataatca atcactatgg tctcgacttg accaatcccg
     1981 acgcacagac attcttattc gacaacatca aggggttttt gtttggcgaa gtgccaaacc
     2041 ttgcgcaaat cgatacctcc aaagaaggct ccgttaagtg gtagcctggc tttaaaagtc
     2101 caacgaatat ccttctactc ctcttccggc ccattggttg cagtggcgtc tatatgcgcc
     2161 aatttcaatc gacattcggc gagttcgtcg tgtgtttgtt gccatgtttt gatgcgggcg
     2221 tacgcttttc gaacttgtct ttggtttata tatcaggagg tttcgaagga tgaaaatcca
     2281 agtcacggga ctcatcgcct cagctgcgat gactctcgct ctctcaggct gtcaaccatc
     2341 aactaatgag aatataaatt ccaacgccaa tgcgagtctg gcgagaaaca ctaatacgcc
     2401 gctaccatcg ccttcgccta ctgtaagtga gacgtcgagg cgagctccaa cccgcgaaga
     2461 atacgaacga gataaagaaa gatatcagcg cgaagccaag ggtgcaggga gtaaggttgg
     2521 cactggtcta aatgacggtt ggctctgggt taaaacgaga tttgacctgg cggcagccga
     2581 cgatctgcgc gactcgacgg tcaatgtaga tgtcgacaat ggagtagtca cgcttaatgg
     2641 ttccgttgca acagtcgcgc aaaagaccaa agccgaagcc gtcgccaagt cagttgaagg
     2701 agtaaaaagc gttaagaaca tgctgaaagt tgttgccgca aatagcaacg ctaacacgca
     2761 cgcatctcct acacctcgta aatagttttt ttcttcaaga atgaagttga gagccgcgat
     2821 ttctgaagcg gctcttattt tttcgcttcc ggttccgtaa ccggggcacg ctcgactttt
     2881 tggcggtttc aagtttcctt caatccaatt ttagtttaag ctctcacctc tcttacctaa
     2941 atgggagttg ctaccgggtc ttcttaaccg gtcttacgct tttcgcaaac caacttgatg
     3001 gggatttggt tgactatgcc aaaggtccgc ctgcgactat tcgtcgctat cagcgctcta
     3061 attttcgtcg ttatccactc gttttcttca actaaggctc agcgctcggg cgtagatgtt
     3121 tacgcaatca caaatgctcg catcgtaacc gtctctggac cggcaatcga caaaggtacc
     3181 atcgtcgtac gaaacggtct gatcgcggcg gttggatcca atgtcactgc gccacctgat
     3241 gcccgagtga tcgacgcaac cggcttgact gtctatccgg gattgattga ctcgtacacc
     3301 aatctggctt tgcccgagcc cgcccctact ccttctccta cgggtggcgg tggtgctgcg
     3361 ttttttctcg cagcccctcg tcccagtccc ggtggtccaa attcaactca gccggccggc
     3421 cttcaaccgg aagtcatggt tgaggacgtc attcgcaccg gagcttcaga tctggaagct
     3481 tcacgcaatg ctgggatcac ctctgcgctg acacttccca gaaccggcat atggctaggt
     3541 caatcagctt tcataaatct tgccggagat actccgcaac agatgatcgt acgctcgcca
     3601 gtggcgatgc acgttggctt tactcctctg cgcggaactt atccagggtc gctcatgggt
     3661 gttttcgcga ctttgaggca gatgatgctc gacgctcaac gctaccgcga ctcgaggcag
     3721 atttacgagc gtaacccgaa tggcattcgc cgacccgagg tcgatagatc gctggctgcg
     3781 cttgtaccgg tggtcgacgg caaaatgcca gttgttatgt atgcaaatac agaacgcgaa
     3841 attgatcggg ccctcgatct tacaagtgaa ttccagctga aaacgattat cgcaggcggt
     3901 agagaatcgg gacgattggc aggtcgattg gcccagatga aggtgccagt cttgctttct
     3961 ttgaatctcc cgcgccggac cactgcagcc cttccagagg cagatcccga gccgttgcgc
     4021 gtcctccggg aaagggtgga ggctcaacaa accgcaggta agttagttaa ggccggggtt
     4081 aggtttggtt ttcagtcagg ctcgatgacg aatatggctg acctgttatc aaacgccgga
     4141 aaaaccatcg aagccggtct gctgagcgac gatgccgttc gagcatttac tatctggccg
     4201 gcagaaattc tcggcgtcag caatcagtta ggttcgattg agcaaggcaa gatcgcaaac
     4261 ttcaccataa cgcgtggcga tctcttcgat cgcaacagtc gtatcgctca cgtctttatc
     4321 gatggacgcg agatcgacct ccggccctcg acaggtggac caacaacgcg aagtactctc
     4381 accggtacgt ggaccgtaac agtcaacctc ggaacggaca cgaaaacgat caccttgaac
     4441 cttcaacagg aaggcgaacg attacgcgga tcgatcgagg gtccactggg ctcagcggag
     4501 atcgcaaacg cttcgaccat gcctgaactt cgtttcacgg ttcccgtaaa tatcgaagga
     4561 caaaccaaag aggccacgtt cgtttccact tcatcaggta atgagcttcg cggaaccgtg
     4621 actgtcgaag gtggtacgcc aggaacgttc acagcaactc ggaatagtca gcctaattga
     4681 ttcgccagat tcttaccgtt cggaactcat ggagttaatt acgatgaaaa tcttgagagg
     4741 atttttaatt gtcataataa cgatggtagc tgctagttat tacgacgggg acgtctttag
     4801 ccagaatcct tcggaaacgc tgattcgcaa cgctacagtg cttaccgtaa ctcgcggcac
     4861 cttgcagaac actgatgtgt tacttcgcaa cggcaagatc gctgctgtcg ggaaaaacct
     4921 gaacgcttca tcgaatgcgc gaatcattga tgcgaccgga aaatttgtca tgccgggcat
     4981 catcgactgt cactcgcatt caatgctgga cacaattaac gaaggaacgc tcgctgttac
     5041 ttcgatggtc cgcacccgcg atgttttgaa cgttacggat gtcgatctct accgcgagct
     5101 tgcgggtggc gtcacaactc tgaatttgct tcacggttca gcaaatccta taggtggttt
     5161 gaacacggta gtaaagatca aatatggacg ccccgccgag gagtttatct ttcccggtgc
     5221 aatgcccgga attaagttcg cactgggcga gaatgtaaag cgctcaagca cgccaaatca
     5281 gccggggatc actcgtcgtt atccaaatac acgaatgggt gttgaggaaa caattcgaga
     5341 cgcctttact cgtgctcgag actacaaacg cgcctgggat gagtatcgag cggctgtcgc
     5401 gaaaggcgac aaaaacatcc ttccgccaag acgagatctg cagttggaac cactggtaga
     5461 aatactcgag ggcaaacgct acgtgcatgc tcactgttac gtcgccagcg agattctgat
     5521 gttgatcaat ctcgccgacg agtttggctt taagatcaga acatttcagc acgtcctcga
     5581 aggctacaaa gtcgccaaag aaattgcgca acacggggca ggcgcctcaa cgtttgccga
     5641 ctcgtgggcg tataagatcg aggcctacga cgcaattgcc tacaacactg ccatcatgac
     5701 tcgggcgggt atcgtcgtat cagtaaactc agactctgat gaacgggctc gtcgtttgaa
     5761 catcgacgcg gccaaagcaa tgcacttcgg agatctgact gaagagcagg cccttaaact
     5821 aattacttta aacccggcga ttcaactcgg aattcaagct cgcgttggtt cgatcgaagt
     5881 aggcaaagac gcggatgtgg cg
//
LOCUS       DASSFP010000318         7758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_983636, whole genome shotgun sequence.
ACCESSION   DASSFP010000318 DASSFP010000000
VERSION     DASSFP010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983636"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFH91_07085"
     CDS             <1..400
                     /locus_tag="VFH91_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862793.1"
                     /translation="ALYYRGEVAAALHVLEQVLEKHPQMDGMRPILAICLSAMGQHTK
                     ANEQLTHKVRMVAEADYDISYWLASAYLLQGRQVEALRWLETAIRLGNENYPWFESDP
                     NWTDMHEDPRFVELMKRIKDQRAHSGDLSE"
     gene            397..1197
                     /locus_tag="VFH91_07090"
     CDS             397..1197
                     /locus_tag="VFH91_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6862794.1"
                     /translation="MKTFVVGDIHGRCAQLLNLLDMLPRDPVNDSLIFLGDLIDRGAD
                     APGCVDHVVNLCHENPQNVVCLRGNHEQMLMDFIDGINNVWLSPVVGGEKTFEQYTGR
                     SVQIDSEEDLKELHRDLATHLPAKHLEFIRQTPFFHEDEFAIYVHAGLEEGKHPQESS
                     EVSLLWLRNMDFFKNYRGKPCVFGHTPTPLLPLRGRLGRHGIYISHSAVGLDSGYNHD
                     SPLSCLSLPDFNLYQTYADGREETHQITSFIPEALKEMQRKAGFSNKE"
     gene            complement(1344..2258)
                     /locus_tag="VFH91_07095"
     CDS             complement(1344..2258)
                     /locus_tag="VFH91_07095"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET6862795.1"
                     /translation="MSSDVMAQSALELEFLMARNRSLVEVELNRLLPSNAVSPERVNQ
                     AIRWSVFAGGKRFRPALLLATGQTFGASIESLLNSACAFEMIHTYSLIHDDLPSMDDD
                     ELRRGRPTCHIRFDEATAILAGDALQTLAFQTIAQDDFLTDRIKVGLIAELAKASGTP
                     EGMVAGQALDIEAEARQVSAGELEEIHRLKTGALIVAAARAGALIAEASANDLSSVSE
                     YAAQLGLLFQITDDLLDVTATAEDLGKTPGKDARSAKATYPGLYGLEETKAAASLAHT
                     AACAALAKIDRPTDTLRLIADYILNRPK"
     gene            complement(2248..2526)
                     /locus_tag="VFH91_07100"
     CDS             complement(2248..2526)
                     /locus_tag="VFH91_07100"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6862796.1"
                     /translation="MKNMEESPRSFEASLEALEKIVHQLENGDLPLEKSLDLFEQGIR
                     LSRECQERLNQAERRIEVLLRDNQGRTVVSQYNEPVETPGDREDENVF"
     gene            complement(2541..3275)
                     /locus_tag="VFH91_07105"
     CDS             complement(2541..3275)
                     /locus_tag="VFH91_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862797.1"
                     /translation="MEQEPVRKRIVINLDGPPGARPSAGTRRRRWPKVLAVLALLLLI
                     GVVVVVAGAFFGWRYYQSTPSYSVALILDAAQRNDMPELEKFIDDEAISQNLLKAVSE
                     KAAGRYGSALSGTVQSQIDNVLPSLMPSVRQIVRDEVAKEVKEFASKSEPKPFLFVAL
                     AVPSFVTLKTEGDTAHASAPISDRTMEFTLKRNADRWKIVEVKDDTLTQKVVDGVVKE
                     LPAIGGPDKNTATPRVKPRRSRGRNR"
     gene            complement(3287..4687)
                     /gene="xseA"
                     /locus_tag="VFH91_07110"
     CDS             complement(3287..4687)
                     /gene="xseA"
                     /locus_tag="VFH91_07110"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6862798.1"
                     /translation="MNQPLFNALFDKTERRPLTVSELTNSVKTTIESRFPSVWVEGEI
                     SNFKQHSSGHWYFTLKDSRSQLRAKCFRSSNIRIRFKPVDGLHVRARGKLSIYAPRGE
                     YELVVETLDPVGAGALQIAFEQLRDRLSAEGLFARELKRPLPVFPRRVGVVTSPTGAA
                     IRDILNVIARRTRTVHVLFSPAKVQGESAGADIARAIRFINQYHERAVREGRTQDQVD
                     VLIVGRGGGSTEDLWAFNTEEVARAIRNSGIPVISAVGHETDTTIADFVADHRAPTPS
                     AAAEMVAAHEGQICAALEQLGRQLIRLTRFKVVDARSRVQEQVLSQAFDEVKARMRNA
                     RLATTSANHRLENAMGQSLRHSQSRSNAIALKLSPAELQRRMSMVKSQFEAVSLAFTN
                     AVDDRVQKGRERLAVAAASLDALSPLAVLQRGYAIAQDSNGKLIRDATTIAVGQSVKV
                     RVNKGKLHTRVERVEE"
     gene            complement(4693..6426)
                     /locus_tag="VFH91_07115"
     CDS             complement(4693..6426)
                     /locus_tag="VFH91_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6862799.1"
                     /translation="MFFQKYPANSISRRSSWPKIIFICAIVLLIVGIDQAVSAQTYQR
                     ELDTPDVVSLVVKTKNGKVSIVVADDQQKKVTIQATSNGQPVEAGDLTVENKGEHISI
                     AVRARRDEDRIDLTVRVPPRSRISVESLAGAVDVVGNVESAEVKTDTGTIHADVPLDA
                     LKFDFVWEASRPRFMSDVPLPEIKERAGGWFRLSGNLGEKKPPKEQKVALDFRTQRGV
                     VLLNVDPNLAPADLRERPLTEAARAIVRSGDSQLVDAIRRVSPKMFGDYAKTLPPLEK
                     EPTLRKGLPPGQFVSRVAPQLLRFNISVMDRNGRAIGGMRESDFTVFENGVERRVTSV
                     APANEPFNLVLLLDVSGSVEERMDFIRKAARDFLRTVSPQDRISIISFRDDIQVISDF
                     STDRQMLSRKLDQIDAGGGTALYDALGYVLASTLKELRGERTAIVVMSDGDDNKSFVP
                     FPSILEALTESGALVYPLYVPSGLIPESSVPKPEVTIDPLRTRYLTLTTRAEEEGRKL
                     ATASGGLYYPIRRIEDLQKAYDDVVLQLRSAYTITYASNSVSSTLPRVRVRTNRDGAS
                     IRLSPVVEIAP"
     gene            7034..>7758
                     /locus_tag="VFH91_07120"
     CDS             7034..>7758
                     /locus_tag="VFH91_07120"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET6862800.1"
                     /translation="MAQREPVRENRLYIHNLVKRDPRAGLAEDVRKGLTAQPKRFLPK
                     YFYDELGSQLFEAICLLPEYYLTRAENEILERYSDEIAAAVPGNKTLLEMGSGSASKT
                     RLVIEAVLRRQQDLLFVPVDISATALESSSRILLQSYPRLRIEAYAADYFAGLAELGK
                     KLRGRTLALFLGSNISNFEEDEALRFLRAMRKVLKSGDALLLGADLKKDPAILEAAYN
                     DALGVTSAFNLNVLARINRELGGT"
BASE COUNT         1834 a         2052 c         1950 g         1922 t
ORIGIN      
        1 agcactttac taccgcggag aggttgctgc ggcattacac gttctggaac aagtactgga
       61 aaaacatccg caaatggatg gtatgcgtcc aatcctggca atttgcctga gcgcgatggg
      121 ccaacacacg aaagccaatg aacaattgac tcataaagtc agaatggtgg ccgaggcaga
      181 ttacgacatc tcttattggt tggcttccgc ctacctgtta cagggacgac aagtggaagc
      241 gctacgctgg ctggaaactg ccattcgatt aggtaatgaa aattatccgt ggttcgagtc
      301 cgatccaaat tggactgaca tgcacgagga tcctcgcttc gttgaattaa tgaagcgcat
      361 aaaagatcaa cgcgctcata gcggggacct ttctgaatga aaacttttgt agtcggcgac
      421 atccatgggc gttgcgctca gctgctaaat ctgctggata tgttgccgcg ggatcctgtc
      481 aacgactcgt tgatattttt aggagacctg atcgaccgtg gtgctgatgc tcccgggtgc
      541 gtggaccacg tcgtcaatct gtgtcatgag aacccgcaaa atgttgtttg cttgcgtgga
      601 aatcacgagc agatgttgat ggattttatt gatggcatta acaacgtctg gctttcccct
      661 gttgttggtg gcgaaaaaac atttgaacaa tacactggac gctcagttca gatcgatagc
      721 gaagaagatc tgaaagagtt gcaccgcgat ttggcgacac atttaccagc caaacatctc
      781 gagttcatcc gccagacgcc gttctttcat gaagacgaat ttgcgatcta tgtgcacgct
      841 ggactcgaag aaggtaaaca tccgcaagag agttcggaag tttccctgtt gtggttgcgg
      901 aacatggact tcttcaaaaa ctatcgcggc aagccatgcg tgtttggtca cacgcctact
      961 ccacttttgc ccttacgagg gaggttaggt cgtcatggca tttacatatc gcacagcgcc
     1021 gtcggcctcg acagtggcta caatcacgat tcaccgctct cctgtctatc gttgcctgat
     1081 ttcaatcttt accagaccta cgctgatggt cgtgaagaga ctcaccagat cacgtcgttc
     1141 attcctgaag ctctaaagga aatgcaacgc aaagccggct tctcaaataa agagtaaccc
     1201 tccagaatga tgcagttggg aaggggccag agctcagggg tcagggctga taggacctat
     1261 atgacctata ggacctatca accctgaccc tgacccctgc accattccaa ctgcatgatt
     1321 ctggcgttta gtgttttcta attttacttg gggcgattga ggatgtagtc ggcgattaga
     1381 cgtagcgtgt cggtgggtcg atcaattttt gccagagcag cacaggccgc cgtgtgagcc
     1441 aggctcgcgg cagccttcgt ttcctccaat ccgtacaacc ccggataagt ggctttagcc
     1501 gaacgtgcgt cctttcctgg tgtctttcca aggtcttcag ctgttgcagt cacgtccaac
     1561 aggtcatcgg taatctggaa caacaatcct aactgcgctg catattctga aactgagctc
     1621 agatcatttg cgcttgcttc agcgattaga gctcccgcac gggctgccgc aactatcaaa
     1681 gcacctgtct tcaatcgatg aatctcttct aactcacccg ccgaaacctg gcgcgcctcc
     1741 gcttctatgt cgagtgcttg tccggcaacc attccctccg gagttcctga cgcctttgct
     1801 agttctgcaa tcagacccac ttttattcga tccgtcagga agtcatcttg cgcgattgtt
     1861 tgaaatgcaa gtgtttgaag agcgtcgccc gccaggattg ctgttgcttc atcaaaacga
     1921 atgtggcatg tcggccttcc ccgtcttagc tcgtcgtcat ccatcgaagg aaggtcatcg
     1981 tggatcagcg agtaggtatg aatcatctcg aaagcgcagg cgctattcaa cagagactca
     2041 atacttgctc caaaagtctg gcctgttgcc aggagcaaag cagggcgaaa gcgtttgcca
     2101 cccgcaaata cactccaacg aatcgcctgg tttactcgtt caggagaaac agcgttggaa
     2161 ggtaaaagtc ggttcagctc gacttcgact agagaccggt tgcgagccat gaggaactca
     2221 agctcgagcg cgctttgggc catcacatca gaagacattc tcatcctcgc ggtcgcctgg
     2281 tgtttcgact ggctcgttgt attgacttac aacagtgcgg ccctgattgt ctcgcagtaa
     2341 aacttctatc cggcgttcgg cttgattgag ccgttcctga cactctcgtg acagtcgaat
     2401 tccctgctca aagagatcca gactcttttc aagtggcaga tcaccgttct ccaactggtg
     2461 tacgattttt tcgagcgctt cgagtgaggc ctcgaagctg cgtggtgact cttccatatt
     2521 cttcatcgcg tctttcgttt ctatctgttt cggcctcgtg accggcgcgg cttgaccctg
     2581 ggtgtcgcag tatttttatc cgggccacca atcgccggta attcctttac gacgccatcg
     2641 acaacttttt gcgtgagggt gtcgtccttt acttcaacga ttttccatcg atcggcgttg
     2701 cgcttcaaag tgaactccat agttcggtct gaaatcgggg ctgacgcgtg ggcagtatct
     2761 ccttcagttt tcagtgtgac aaaggatgga actgcgagag caacaaacag gaaaggctta
     2821 ggctctgatt tcgacgcaaa ttcctttacc tctttggcaa cttcatctcg aactatctgt
     2881 cgtacactcg gcattagtga cggcaacaca ttatcaatct gcgactgaac cgtaccactc
     2941 aaagcacttc cgtatcgacc agcggctttc tcgctgacgg ctttcagcaa attttggctg
     3001 atcgcttcat cgtcgataaa tttttcaagc tcaggcatat cgttccgctg agcagcgtcg
     3061 aggattagtg caaccgagta agaaggtgtg gactgataat agcgccagcc aaaaaaagcg
     3121 ccagctacca cgacaaccac tccaatcaat aaaagcagcg caagcaccgc caacactttg
     3181 ggccatctgc ggcgacgagt gccggccgat ggacgagctc ctggtgggcc atccaggttt
     3241 atgacgatgc gtttacggac cggctcttgt tccatatgtt cgatcgttat tcttcgacac
     3301 gttctacacg agtgtgaagt tttcctttat tcacacggac cttcacgctt tgaccgacag
     3361 ctatcgtggt tgcgtcgcga atcaactttc cattggaatc ctgggcgatc gcatatccgc
     3421 gttgcagcac tgcaagtggc gacaaggcgt cgagcgaagc agcggcgact gcgagacgtt
     3481 cgcgcccctt ttgtacacga tcgtcgacag cgtttgtaaa cgcaagtgac accgcttcaa
     3541 actgtgactt aaccatcgac attcgtcttt gcaactctgc cggcgacagc ttcaacgcta
     3601 tcgcattgct gcggctctgg gagtgccgca atgattggcc catcgcattc tcgagtcgat
     3661 ggtttgcgga agtagtagcg aggcgagcgt tcctcatgcg cgccttgacc tcatcgaatg
     3721 cctgggaaag tacctgttcc tgaacgcgcg aacgagcatc gaccacttta aaccgcgtga
     3781 gtctaatcag ctgtctccca agttgctcca gcgcggcgca gatttgacct tcatgggccg
     3841 ccaccatttc ggccgctgct gagggtgtcg gcgcccgatg gtccgcgaca aagtcggcta
     3901 tcgtggtgtc tgtttcgtgc ccaacggcgg agatgacggg tataccggag tttcgtatcg
     3961 cccgggcaac ctcctcggta ttgaaggccc agagatcctc tgtcgagcct ccgcctcgcc
     4021 ctacaattaa gacatccacc tggtcctgtg tgcgaccctc gcgtactgcc cgctcatgat
     4081 actgattgat gaatctgatc gctcgcgcta tgtctgcacc ggcggattcg ccctgaactt
     4141 ttgccggaga aaatagtaca tggacagtcc tcgtcctgcg agcgatgacg ttcagtatgt
     4201 cgcgaatagc agcacccgtc ggcgaagtca ccactccaac tcgtcgagga aaaacaggca
     4261 acggtcgttt tagttcgcgc gcaaacaaac cttcggcgct caagcgatct cgtagttgtt
     4321 cgaaagcgat ttgaagagca ccggctccga cgggatcgag agtttcaaca actaactcat
     4381 actcgccacg cggcgcgtag atcgacaatt ttcctcttgc tcgcacatgc aaaccatcga
     4441 ctggcttgaa acgaattcga atgtttgagg atctgaagca cttcgcacgt aattgcgagc
     4501 gtgaatcctt caaagtgaag taccagtgac cagaagagtg ttgtttgaag tttgaaatct
     4561 caccctcgac ccacacggac ggaaagcggg actcaatggt ggttttaacg gaattcgtaa
     4621 gctccgagac cgtaagtggt cgtcgctccg tcttatcgaa aagagcattg aagaggggtt
     4681 gattcattct agctaaggcg caatttccac aaccgggctc aatctaattg aggccccgtc
     4741 tcgattggtg cgaacccgaa ctcgtggcaa tgtcgatgag acagaatttg atgcataagt
     4801 gatcgtgtat gcgctccgta gttgtaacac gacatcatcg taagctttct gcaagtcctc
     4861 aatccggcgg attggataat agagtccccc cgacgcagtg gcgagtttgc gaccctcctc
     4921 ctcggcgcgt gtagtcagcg taagatatcg agttcgcagg ggatcaatcg tcacttcggg
     4981 tttcggaacg ctgctttccg gaattaatcc tgaaggtacg taaagaggat atacaagcgc
     5041 tcctgattct gttagcgcct caaggataga agggaaagga acaaacgact tgttgtcatc
     5101 accgtctgac atcaccacga tggcagttct ttctcctcgc aattccttga gagtagaagc
     5161 cagcacataa ccgagcgcgt cgtagagagc tgtgccgcca ccggcgtcga tctgatcaag
     5221 ttttcgcgaa agcatctgcc ggtcagtgct gaagtcggag attacttgaa tatcatcgcg
     5281 aaagctgatg atcgaaattc gatcttgagg actaacggtc cgcagaaaat ctctcgctgc
     5341 tttgcgaatg aagtccatgc gctcttcaac actgccagac acatctaaaa ggagaaccag
     5401 gttgaacggt tcgttagctg gagccacgct agttacacgg cgttccaccc cattttcaaa
     5461 gaccgtgaag tcagactcgc gcataccacc aatcgctcga ccattgcgat ccattacgct
     5521 tatgttgaat ctaagcagct gcggcgcaac tctactgaca aactgtcccg gaggtaaacc
     5581 tttgcgaagg gtcggttcct tctcaagtgg cggcagcgtc tttgcgtagt cgccaaacat
     5641 ttttggggaa actcttcgga ttgcatcaac aagttgacta tcaccacttc gaacaatcgc
     5701 gcgtgctgct tcagttaaag ggcgttcgcg caaatccgcc ggagcaagat tcggatctac
     5761 atttaggagt acgacgcccc gttgagttcg aaaatccaga gcgacctttt gttcttttgg
     5821 cggcttcttt tctccgagat tgcctgagag ccggaaccaa cctccggctc tttctttaat
     5881 ctccggcagt gggacgtcac tcataaatct tggtcggctg gcttcccaaa caaaatcaaa
     5941 cttcaacgca tccagcggaa catcagcgtg aatggtgccg gtgtcagttt tcacctctgc
     6001 cgactctaca ttcccgacta catcaacagc gcccgcaaga ctctcaaccg agatcctgga
     6061 acgaggcgga acccgaacag tgagatcgat tcggtcttcg tcacggcggg ccctgacggc
     6121 gatcgagata tgttcacctt tattctcgac agtcagatct cccgcttcga ctggttgtcc
     6181 attcgatgtg gcctggatcg ttactttttt ctgctggtca tcggcaacga cgatgctcac
     6241 ttttccgttt tttgtcttta ccacgagcga gacaacgtca ggggtgtcca gttcgcgttg
     6301 ataggtttgg gcagagaccg cttgatcgat tccgactatc aataaaacga tggcgcaaat
     6361 gaaaataatt ttcggccacg aagatcttcg acttatgctg tttgcagggt atttctggaa
     6421 gaacatcgcg accgatcttg cccacacagc gcccgagggt caagtgaggt cgctgttaaa
     6481 tgaagatgaa aacccccaac gccaaactaa tgtaaatcgc aggtagaaag gggcagtacc
     6541 ggatcgccgt gactttgcct caaaagtctg agatctgacc tctcatacac accgggcttc
     6601 agcccggtga ccaagcgcaa gaaattagtg gaaccgtttt aacggtttgc tcttcttctc
     6661 aaggccccga ggccgttgac cacgaagcag ttggctgatt tagccgaacg gagaaaccgt
     6721 tgaaacggtt tcttgatctc gttgtagcgc gtgatcaccg ggctgaagcc cggtgagaat
     6781 gagaggtaag atttaagact tttgggacaa agccgatcgc ggtagtgtcg ggctgagtct
     6841 cgaattattg agttgatgat attttgggga cgcacccatt gctatcgcga cccggtactg
     6901 acccgtcata gaccgcaaaa gccccatctc gggtgttgtt aactgccctt gatcggctat
     6961 cataccggga ccgaacaatc cgaagccccc aggttaactg ctcagtcgcc cacttatcga
     7021 ccaggagtat tcgttggcac aaagagaacc agttagggaa aaccgccttt atatccataa
     7081 ccttgtcaaa cgggatccgc gggctgggct ggctgaagat gttcgaaaag gattgaccgc
     7141 gcagccaaaa cgctttctcc caaagtactt ttatgacgaa ctcggctcgc aactttttga
     7201 ggcgatctgt ctcttaccag aatactattt gactcgcgcc gagaacgaga ttcttgaaag
     7261 atactccgac gaaatcgctg ctgccgtccc tggcaacaag acacttctgg aaatgggaag
     7321 cggtagcgca tcgaaaacgc gcctcgtcat tgaagcggta ctccgacggc aacaagacct
     7381 actctttgtc ccggtcgata tatccgcgac agcacttgaa agtagttccc gcattcttct
     7441 tcagtcgtac ccacgtttaa gaattgaagc gtacgcagct gattacttcg ccggacttgc
     7501 cgaactcggt aagaaactga gaggtcgcac tctggctctc tttcttggtt cgaacatcag
     7561 taactttgaa gaggacgaag cgcttcgctt cctgcgtgca atgcgaaagg tattaaagag
     7621 tggcgacgcc ctgttgcttg gagctgactt aaaaaaggat ccagcgatcc tggaagctgc
     7681 ctataacgat gcccttggtg tgacgtccgc ttttaatctg aacgttctgg cgcgcatcaa
     7741 tcgtgaactc ggtgggac
//
LOCUS       DASSFP010000319         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1278800, whole genome shotgun sequence.
ACCESSION   DASSFP010000319 DASSFP010000000
VERSION     DASSFP010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278800"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2372)
                     /locus_tag="VFH91_07125"
     CDS             complement(<1..2372)
                     /locus_tag="VFH91_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6862801.1"
                     /translation="MSTTLSRNSTLLLLCAAPVCLLVLVWGAINTLSLPLSSPLGLWS
                     FVGITLLSVITAGFSVAVTGDDRRRWSHKSVADAFVLLAIIMYSGTVSGNAGPAVILA
                     GLTAVSACYGRYSRRAIVGIVCVSVISTLLTAWVYAAVVDLFAGSTNLAGDSGIQFKT
                     FLFPLLLLAALQYFLSTVITAWFLSFEAGEFKLLPSPESIVWTGAAQLAALGSAVLIY
                     SAIQTADVLHAFTGVLTSAIVYLLYHFNEKRLDEVRRAEAERRRHSEEMATIHMNTIE
                     SLAIAIDAKDQTTHGHVRRTQIYAEQMGKLLKVSQLEVQALLAGALLHDIGKLAVPEY
                     ILNKPGKLTEAEFAKMKIHPTVGGDILKRVNFPYPVEDIVRYHHEKWDGSGYPKGLKG
                     EAIPLTARIISVVDFYDATRCDRPYRKGMKREDSLALLRGMIGSAFDPKVVQVFIDHV
                     EEFDCLINAEDIQEQVPSEIPELDLETKTRPDAGLASDVMGTQEERVDGFRSISDAQR
                     EVFALHEIAQTIGSSLNLSDTVALVSNKLRAIVPFDSCVIFVVDERSGKAVAAHAAGD
                     HHDVFSRRKINIGEGITGWVVANSRSMCNASAELDLVGVPDSVAKQFRGILVSPLLRE
                     DGAFGAIALYSKARTSYTTEHVRLLESVCQHASSALNNALTYEKTKESALVDPLTDLP
                     NARGFYMMLEQKIAECQRLKRESLAVISMDLDEFKAINDRYGHSTGDRLLASIAGVIR
                     RELRQMDILTRYAGDEFVAIMPMASNNMAQSVGERMRNTVEEQLFSVRPGT"
BASE COUNT          680 a          723 c          715 g          674 t
ORIGIN      
        1 gttccgggac gaacggaaaa cagttgctct tcgaccgtgt ttctcattcg ttcaccaacg
       61 gactgggcca tgttgttcga ggccatcggc ataatggcta cgaattcatc gcctgcgtaa
      121 cgagtgagaa tatccatttg tctaagctcg cgtcggatca cacccgcaat gcttgcgagc
      181 aatctatccc cggtcgagtg gccgtatctg tcattgatgg ccttaaactc gtctaaatcc
      241 atactaatga cggcaaggga ttcacgtttc agccgctggc attctgcgat cttctgttcg
      301 agcatcatgt agaagccgcg agcgttcgga agatcagtta gcggatctac caacgcactc
      361 tctttggtct tttcataagt caacgcattg ttgagtgcgc tcgatgcatg ttgacacacc
      421 gactctaaca aacgtacgtg ttcagtagtg taagacgtac gtgctttcga atagagcgcg
      481 atcgcaccaa aagcgccatc ttcccgcagt aacggagaaa ccagtattcc tcgaaattgt
      541 ttggccacgc tgtccggtac gccgaccagg tctaactcag ccgacgcgtt gcacatcgag
      601 cgggaatttg cgaccaccca accggttatt ccttccccga tgtttatctt gcggcgcgag
      661 aacacgtcat ggtgatcacc ggccgcgtgc gctgcgaccg ccttacctga acgttcgtct
      721 actacaaaga ttacgcaact atcgaatgga acgatcgctc gcaacttgtt actgaccagt
      781 gccacggtat cactgaggtt gagtgaggat ccgattgttt gggcgatttc gtgtaaggca
      841 aatacttcac gttgtgcgtc actgatcgag cgaaacccgt cgactcgttc ttcctgagtt
      901 cccattacat cggacgcgag acccgcatcc gggcgagtct tcgtctcgag atccagctcg
      961 ggtatttcag acggaacctg ttcctggatg tcctctgcgt taatcaaaca gtcaaactct
     1021 tcgacgtgat cgataaagac ctggaccact ttgggatcga atgcggagcc aatcatgcct
     1081 cgcagtagcg cgagtgagtc ttcacgcttc atcccttttc gatagggacg atcgcagcga
     1141 gtagcgtcgt agaagtccac gacagaaata attctcgcag tcaagggaat cgcttcaccc
     1201 ttgaggcctt tgggatagcc actaccatcc catttttcat ggtgatatcg aacaatgtct
     1261 tccaccgggt agggaaagtt gacgcgtttg aggatgtctc cacccactgt cgggtgaatt
     1321 ttcatcttcg caaactctgc ttcggtgagc tttcccggtt tgttcagaat gtattccggt
     1381 acggctaact taccgatgtc gtgaagtaat gcgccagcga gcaaagcctg aacttccagc
     1441 tgtgaaactt tgagcaactt gcccatttgc tccgcgtaga tttgagtgcg tcgcacatgt
     1501 ccgtgagtcg tttggtcttt ggcgtcgatt gcaattgcca gcgattcaat tgtgttcatg
     1561 tgaatcgtgg ccatctcttc tgaatgtcgc ctccgctcag cctcagcgcg cctcacttcg
     1621 tccagtcgtt tctcattgaa gtgatacagg agataaacga ttgcagatgt caggacaccc
     1681 gtaaaagcat gaagaacgtc ggctgtttga atcgctgagt agatgagtac tgcggatccc
     1741 agtgctgcaa gttgtgccgc tcctgtccaa acaatacttt caggtgaggg aagcagcttg
     1801 aactctccag cttcaaagct tagaaaccag gcagttatca cagtgctgag gaagtattga
     1861 agagccgcca gtagtaggag aggaaataaa aaagttttga actgtattcc actgtccccc
     1921 gcgagattgg ttgagccggc gaaaagatcg accactgcgg cgtaaaccca tgcggtcagc
     1981 aaagtggaaa ttaccgaaac acacacaatc ccaactatcg cacggcggga ataacgacca
     2041 tagcaggcgg aaacggctgt caaacccgcc aggatcacag ccgggccggc gttaccgctc
     2101 accgtccctg agtacattat tattgcgagt agtacaaaag cgtccgcgac tgatttatgc
     2161 gaccatctgc ggcgatcgtc acctgtgacc gcaactgaga atcctgccgt aataacggag
     2221 agaagagtaa tgccaacaaa tgaccacaaa cccagcggtg acgacaaggg aaggcttagc
     2281 gtgttaatcg caccccaaac gagcacgagt agacaaacag gcgcagcgca cagcaagaga
     2341 agcgtgctat tccgactgag ggtggtcgac atctcggggc tcacatcctt cctgaaaggt
     2401 agcggatcag attgcttgac tactttttcc ggctcaattt aaaagcgggg aaggctctac
     2461 cagcgcgtta gtgcactacg taatcgacat tgtgacttgg gtttgcgagg tttggccgca
     2521 cctaaccttc gcaggtaagc gtgcccaaca ctgtttggca caggtttaac tatagcggcg
     2581 tcagaaggtt gccgtcaaga ttgggaaggg tcaaagagcc agggcggaga ccttccaact
     2641 gcatgatttt taagagttga ctgtcctggg caattctcac tttgcgagga gttggtccac
     2701 aaatgttccg agatcagttg ttggaaatct gcaggtgtag ttctcacaaa cataggcggt
     2761 aaccggggca ccttcagtag ctgtccgatt ct
//
LOCUS       DASSFP010000320         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1861214, whole genome shotgun sequence.
ACCESSION   DASSFP010000320 DASSFP010000000
VERSION     DASSFP010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861214"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2389
                     /locus_tag="VFH91_07130"
     CDS             <1..2389
                     /locus_tag="VFH91_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862802.1"
                     /translation="NSIVNRFNYGFTRASFTQNGDSNENRVNFRFIFSPAAFARTLRR
                     TTPVHNFVDDVSWLRGNHTIGFGGNIRLITNNRISLTPSFDQALINPSFYNESGDVII
                     QPFSDFVSSNDLRDALTSVIGRYSEYSANLVYGANGTLEPVGSPSVRAFATQEYEFYG
                     QDSWRIRPNLTFNYGLRWSTSTPVYEKNGLQVVPNVNLTDYFNQRVASSNSGVPFNDP
                     ITFVLGGKANNAPGYYKQDWNNFAPSLSFAWSPDFGKNWFGKVFGRENKSVIRGGFRV
                     TYDRIGSQLAVNFDLNNLAGFTSARNINANTFDVIPGELGPLFNGFNPDVRALPFPGS
                     TGPIPTSLNFPLTVPSDEDQRIETSLDAGITTPYNTSFNLSYARELGRGLTFEASYVG
                     RFARSLLATRDIMQLNNIVDPQSGMSYYDAINALVGFRYENRPINSIASMPFFDNLFP
                     FMPEFWGDTSLTPTQAAYAFVAPEALGGAENSDYTFLQLQWDDSPNCTTCPFGGGPAK
                     FNNMFYQPQFGALSAFSTVARSNYHALQLSLRERLRDDVTFDFNYTYGHSTDNASGLQ
                     NSTSYGTAFIVNSLYPDRNWATSDFDATHIINANWVVGLPFGRGKKFLNSSSGFVDTL
                     LGGWQLTGIFRWNSGIPFLTPNDCCVWATNWNVQSNGVRTRPLQASTNKTGTPNAFAD
                     PVAAYRSFRNARPGEVGDRNVLRAPSYISLDSGLYKTFKLPWEGHTLQFRWEVFNVTN
                     TQRFDGLTISDLSLGSDPFRGGNPNVDFGRYTSTQTPLNETKAGRVMQFALRYQF"
BASE COUNT          609 a          688 c          639 g          674 t
ORIGIN      
        1 aaactccatt gttaatcgtt tcaactacgg tttcactcga gcgtcgttta cccagaacgg
       61 tgattccaat gagaatcggg tcaatttccg attcatcttt tcaccagcgg cgtttgctcg
      121 cactctgcga agaacgacac cggtccataa tttcgtggac gacgtttcct ggcttcgcgg
      181 aaatcacacg atcgggttcg ggggaaacat tcggttgatt acaaacaacc gcatttcact
      241 gactccttcg tttgatcagg cgcttatcaa cccttcgttc tataacgaat cgggagatgt
      301 gatcatccag cccttctcag acttcgtaag ctcaaatgac cttcgggatg cactcacttc
      361 cgtcatcggg cgttactcgg agtacagcgc aaaccttgtt tatggtgcta atggcacgct
      421 tgaaccggtg ggctcaccct ccgtgcgcgc gttcgcaacc caggaatacg agttttatgg
      481 acaggattcg tggcgtatca gaccaaacct cacgtttaat tacggactcc gttggtccac
      541 gtcgacgccg gtttatgaga aaaatggact acaggttgtt ccgaacgtaa accttacaga
      601 ctacttcaac cagcgtgtcg ccagttcaaa tagtggcgtt ccattcaacg atcccatcac
      661 gttcgtgctc ggtggtaagg ctaataatgc accgggttat tacaaacaag actggaacaa
      721 ctttgctccg tctttgtcct ttgcctggtc gccagacttc ggcaagaact ggtttgggaa
      781 ggtgtttggt cgtgaaaaca agagcgtaat acgtggtggt ttccgcgtga cgtacgatcg
      841 aattggcagt caactcgcag tcaattttga ccttaacaac ctcgcgggct tcacttcggc
      901 gcgcaacatc aatgcgaaca cgttcgatgt aataccgggt gaacttggac cattgttcaa
      961 cggattcaat ccagatgttc gcgctcttcc gtttcctggt tcaactggac caattccaac
     1021 ttctctcaat tttccgctga ccgttccgtc cgatgaagat cagcgaattg agacctctct
     1081 agacgcggga attaccacgc catacaacac tagcttcaac ttgtcgtatg cgcgcgagct
     1141 cggaagaggc ctgacattcg aggccagtta tgttggacgt tttgctcgaa gcttgttggc
     1201 cacgcgcgac atcatgcaac tcaacaacat cgttgacccg cagtcgggta tgagctatta
     1261 cgacgcaatc aacgcgctcg ttggttttcg ctatgagaat cggccgatca attcgattgc
     1321 gtcgatgccg ttctttgata atcttttccc attcatgcct gagttctggg gtgatacctc
     1381 gctgacgcca acccaggcag cctacgcttt tgttgccccg gaagcgctag gcggggcaga
     1441 gaacagtgac tacacgttct tgcagctgca atgggacgac agtcctaact gcaccacgtg
     1501 tccatttggc ggtggtccag cgaagttcaa caacatgttc tatcaaccgc agtttggagc
     1561 gttgtcggct ttctctactg ttgcaagatc gaactatcac gctttgcagc tgagtttgcg
     1621 agaacggttg cgagacgatg tcacgttcga cttcaattac acgtacggac attcaaccga
     1681 caatgcgtca ggactgcaaa actcaaccag ctatggaaca gctttcatcg tcaactcgct
     1741 gtatccggat cgcaactggg cgacttcgga tttcgatgct acacatatca tcaatgccaa
     1801 ctgggtcgtc ggtctacctt tcggtcgagg taagaagttt ctcaacagct cgagcggatt
     1861 cgtagacaca ttgctgggag gatggcagct gaccggtatt ttccgttgga acagcggtat
     1921 accgttccta accccgaatg attgctgcgt ctgggccaca aactggaacg tacaatcgaa
     1981 cggcgttcgc acacggccct tacaggcatc aacgaacaag acaggaactc cgaatgcatt
     2041 tgctgatccg gttgcggcat accgcagctt ccgtaacgcg agacctggtg aggttggcga
     2101 tcgaaacgtc ctacgcgctc cgagctacat ctcgttggac tcggggcttt acaagacctt
     2161 caaacttcct tgggaaggtc acacgctgca gttccggtgg gaggtcttca atgtgactaa
     2221 tactcagcgc ttcgatggtt taactatttc agatctgagc ctcggctcgg accccttccg
     2281 tggtggaaat cccaatgtcg actttggccg ttacacctcg acgcaaactc ctttgaacga
     2341 aaccaaagcg ggacgagtaa tgcagttcgc gctcaggtat cagttctaaa ccaatttgta
     2401 gagcggtgat ctgggtcgga gttaatactc cgacccattt ttttcgttgg tccgttgtca
     2461 gttgtccgtg gtcagttgta cttctgcttg gtactagccg atgaattcgt ggtcgtaaat
     2521 ccaaacacca agaagccatg cgccgatatc aaaagatcct tcgctactgc gcgtgcgata
     2581 aggctgagat tcgaaattta caatggcatg
//
LOCUS       DASSFP010000321         5128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2170523, whole genome shotgun sequence.
ACCESSION   DASSFP010000321 DASSFP010000000
VERSION     DASSFP010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170523"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFH91_07135"
     CDS             complement(<1..418)
                     /locus_tag="VFH91_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862803.1"
                     /translation="MENRVQIQPFSVGNVTFGDGRLTVIAGPCVIESAEHALMMAREC
                     STRARAVGLDYVYKASFDKANRSSIKSFRGLGMDAGLKVLADIKKEVGVPVLTDVHDV
                     SQVAAVAEVVDILQIPAFLCRQTDLIVEAARSGRAVN"
     gene            complement(438..1967)
                     /locus_tag="VFH91_07140"
     CDS             complement(438..1967)
                     /locus_tag="VFH91_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6862804.1"
                     /translation="MVYCPTCGKHYSSDVHACPDDLTPLHVQPEAETQTLIDPLNGTT
                     LDDKYRLDERLGGGGMGNVYKARHLTLDRAVAVKIMHPRLVEDESARIRFEREAKALG
                     RLQHLNAVSVTDFGKTRDGLVYIVMELLEGRTLRDILAREAPLEVARAVSLMLQATAA
                     VAAAHEAGIIHRDLKPANILVTQSTDVPSIVKVLDFGIAKLAAETIDDDHMALTQLGT
                     MLGTPRYMSPEQCDGLELTPAADVYSLGVILYEMLTGMTPFSGPTPLAIAVKQSTEFP
                     RPPREIVASIPEDLERLTLHTLEKRPEDRPPNAAALRSELLETADRLGLEHHALVSTP
                     DIDALREISRESPSGRLVVDLSRVREARALSSGSNEVKVIGGGNTADKARRSKDDRKS
                     KFSRVDVEVPNGFPARTILWAGLTIFVIALSILGFVLLNSNNQPSAPMFLNANESVSP
                     TPKVSPTATPTATPTATPTPLPTSSPGGSVSETKDENKKKDGKGHSFMSRVKRILKKP
                     F"
     gene            complement(2035..3735)
                     /locus_tag="VFH91_07145"
     CDS             complement(2035..3735)
                     /locus_tag="VFH91_07145"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6862805.1"
                     /translation="MMPKYIFVTGGVVSSLGKGLAASSIGCLLENRGLQVALIKLDPY
                     INVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHARLSKANNWTTGRIYLSVIEK
                     ERRGDYLGKTIQVIPHITNEIKDAVRKVAEEDKPDVVIVEIGGTVGDIESLPFLEAIR
                     QMGNEEGSGNAIFIHVTLVPYIAAAGELKTKPTQHSVRDLREIGIAPDILLCRSEKPL
                     SRELRGKIALFCNVKESAVISAQDVDTIYDVPLAFHEQGLDSLIVSQLGLEARAPQVD
                     LSEWRELVATIREPSGGETSIAIVGKYVELEDSYKSLREALTHGGVANNVRVDVKWIE
                     SEELMEDDFEERLRDFDAILVPGGFGKRGVAGMIRAIYYARKTRTPYFGICLGMQTAC
                     IEYARNVSDLKDVDSTEFDLETPNPIIFKLRDLVGVEELGGTMRLGAWPCKLLENSLV
                     QEIYGGAENISERHRHRFEFNPEYRTRLENDGLVFSGVSPDGKFVEMIELPRETHPWF
                     VGCQFHPEYKSKPLNAHPLFAAFVKAAYENRLQTEASIEKNGEVDLIVHERLSATSAD
                     "
     gene            complement(3752..4048)
                     /locus_tag="VFH91_07150"
     CDS             complement(3752..4048)
                     /locus_tag="VFH91_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862806.1"
                     /translation="MSDWIGLAVIVLIVACGIFGLSRLSKPYDISTEEFERRAQEGPG
                     LLGAGLIGLQKALDPAMEKAVEVQEDLREGRYDGEQESGDPPEAGAQDHIETNS"
     gene            complement(4051..4902)
                     /gene="kdsB"
                     /locus_tag="VFH91_07155"
     CDS             complement(4051..4902)
                     /gene="kdsB"
                     /locus_tag="VFH91_07155"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET6862807.1"
                     /translation="MNHAGPNPKNNQSNRVVAVIPARLGSTRLPGKALLPIAGKPMIC
                     WVAERATAARNVNHVIVATDDRKIMEVAQAHGFDSVLTSSLHKSGTDRIAEVAAHLPD
                     CEVVVNVQGDEPLISPITIENAVSAIEREMELANGASIVTSWEPIESADDVLNPDLVK
                     IVLDERDYALSFSRSPIPFPRDAVTKYGSLEKALAGEADLLALFKKHTGLYVYRKDVL
                     LQFTKWEQTKLEKLEALEQLRAMENGVKIKAIKASTFSTGVDTTADFEKVKGIVEDNN
                     PGYVCLA"
BASE COUNT         1236 a         1351 c         1238 g         1303 t
ORIGIN      
        1 tgttgacagc ccgacccgat ctggccgctt caactatcaa atcagtttgc cggcacagga
       61 aggctggtat ctgaaggata tctactactt cggcaacagc agctacttgt gaaacatcat
      121 gaacatcggt cagcacagga actccgactt ccttttttat atctgcaagg accttcagac
      181 cagcatccat ccccagtccg cggaaacttt ttattgacga tcgattcgct ttatcaaatg
      241 atgctttata aacgtagtcg agacctactg cacgcgcacg agtcgaacat tcccgagcca
      301 tcataagagc atgctcagcg ctctcaatca cgcatggtcc tgcgataacg gttaatcggc
      361 catctccaaa agtaacattg cctacggaga atggctggat ctgaactcta ttttccattc
      421 gatgaatcgt ttcctggcta gaagggtttc ttgagtatac gcttgaccct gctcataaac
      481 gaatggccct tgccatcctt tttcttgttt tcatcctttg tttcactaac ggatccgcct
      541 ggcgaactcg tcgggagcgg ggttggcgtt gcggtcggcg tggcggtcgg cgtggcggtt
      601 ggcgagacct ttggcgtggg agaaacactc tcattcgcgt tgaggaacat aggcgcggat
      661 ggttgattat tggagttcag caaaacgaat cccaatatcg ataacgcaat tacaaaaata
      721 gttaagccgg cccacaaaat agtccgcgct ggaaacccgt tgggcacttc gacgtcaacg
      781 cgagagaact tactttttcg gtcatctttc gaccgtctgg ctttgtccgc tgtattgcca
      841 ccaccaataa cctttacctc gttcgatcca gagctaagag cacgcgcttc tcgaacacgg
      901 gaaaggtcga ctacgaggcg accggaagga gactcgcgac ttatttctct caacgcatcg
      961 atgtcagggg tactgaccag agcgtgatgc tcaagaccta accgatctgc cgtctcgaga
     1021 agctcgctgc gaagggctgc agcgtttggt ggcctgtctt caggtcgctt ctcgagcgtg
     1081 tgcagtgtca gccgctccag gtcctctgga attgaggcaa caatctcacg tggcggacgg
     1141 ggaaactcgg tcgattgctt aacagcaatc gccaaaggtg ttggaccact gaacggagtc
     1201 attccggtca acatctcgta aagaatgact cctagactgt agacgtctgc cgccggcgtt
     1261 aactcaagac cgtcacactg ttcaggagac atataacgcg gagtcccaag catagttccg
     1321 agctgagtca aggccatgtg atcgtcatcg atggtttccg cagcgagttt ggcaataccg
     1381 aaatcgagta ctttgacgat tgaaggaaca tccgtgcttt gagttacaag aatgttggca
     1441 ggcttaagat cacgatgaat aattccggcc tcgtgcgctg ccgcaaccgc cgccgttgcc
     1501 tgtagcatca acgacactgc tcgagcaact tcgagagggg cttcgcgcgc gaggatgtcc
     1561 cgcagcgttc gaccctcaag gagttccatc acgatgtaaa caaggccgtc gcgtgtcttt
     1621 ccaaaatccg tgaccgacac ggcattcaaa tgttggagac ggcccagggc ctttgcttct
     1681 cgttcaaatc gaatgcgagc agactcatct tcgacaagtc gaggatgcat gatctttact
     1741 gctaccgcgc ggtcgagtgt tagatggcga gctttgtaga cattgcccat gccgccgcca
     1801 cccagacgct catcgagccg gtacttgtcg tcgagcgttg tgccgtttaa gggatcaatc
     1861 aacgtttgcg tttcagcttc tggctggacg tgtagcggcg ttagatcgtc cggacatgca
     1921 tggacgtcag aagagtagtg ttttccgcat gtgggacaat agaccatcag ttagatgtga
     1981 ctttggatga gagggcgcgg gagattatcg cttaaaccgc cgctaaaagc acacttagtc
     2041 ggcactggtc gcactaagtc tttcgtgaac tataagatcg acctcgccat tcttctctat
     2101 tgaggcttct gtttggagcc ggttctcgta agccgctttc acaaacgctg caaacagagg
     2161 atgagcgttg agcggtttcg acttgtattc cggatgaaat tgacatccga caaaccatgg
     2221 gtgcgtttcc cttggtaact caatcatttc aacgaatttg ccatccggcg aaacgccgct
     2281 gaaaacgagt ccatcatttt ccaaccgcgt ccgatattcg ggattaaatt caaatctgtg
     2341 acgatgcctt tcgctgatgt tctcggctcc accatagatt tcctggacca gagaattttc
     2401 aaggagtttg catggccatg ctcccaaccg cattgttcca cccaactctt caactccaac
     2461 cagatcccta agtttaaaga taatcggatt tggtgtttcc agatcaaact ccgttgaatc
     2521 aacgtctttc aggtcggaca catttctggc gtattcgata caagcagtct gcattccaag
     2581 acaaatacca aaatacggtg tccgggtctt gcgcgcatag tagattgcgc gaatcattcc
     2641 agccacacca cgcttgccaa agccaccggg cactagaatg gcatcgaagt ctcgcaaacg
     2701 ttcttcgaag tcgtcttcca tcagctcttc agactcaatc cacttaacat ccacacgaac
     2761 gttgtttgca acgccaccgt gcgtgagcgc ttcacgaagt gatttataag aatcttccag
     2821 ctcgacgtac ttacctacga ttgcaatcga tgtctcgcca cctgacggtt cccgaatagt
     2881 ggcgaccagt tcgcgccact cggaaagatc aacttgcgga gcccgggctt cgagtccgag
     2941 ctgcgacacg attagactgt ccaaaccctg ctcgtgaaac gcgagcggca cgtcgtaaat
     3001 tgtgtcaaca tcttgagcac ttatgacggc tgactctttg acgttacaaa acaaagctat
     3061 cttgcctcgc aactcgcgcg aaagcggctt ctcggagcga cacaaaagaa tgtctggcgc
     3121 gataccgatc tcccgaaggt cacgcaccga gtgttgcgtg ggcttggtct taagttcacc
     3181 cgcggcagca atataaggga cgagagtgac atgaataaaa atggcgttcc cgctaccttc
     3241 ttcgttcccc atttgccgaa tagcttctaa aaatggaagt gattcgatgt cgccgactgt
     3301 tccgcctatc tcgacaataa cgacgtctgg tttatcttcc tccgctacct ttcttacggc
     3361 gtctttgatc tcatttgtaa tgtgcggaat tacctggatt gttttgccga gatagtcgcc
     3421 gcgacgctcc ttctcaatca ccgaaaggta aattcggccg gtggtccagt tgttcgcttt
     3481 agaaagtcga gcatgagtga agcgctcgta gtgaccaaga tcgagatctg tttcagcgcc
     3541 gtcgtccgtg acaaacacct cgccatgctg aaagggagac atggtgccag gatccacgtt
     3601 gatgtacgga tctaacttga taagggcgac ctggagtcca cggttctcaa gcagacagcc
     3661 aattgaagag gccgccaggc ctttccccag gctcgaaaca actccaccgg ttacgaagat
     3721 atatttgggc atcattttca ccttacaaga tttatgagtt cgtttctatg tggtcctgag
     3781 ctccagcttc gggaggatcg ccagactcct gttcgccatc gtacctacct tcgcgaagat
     3841 cctcctgaac ctccacggcc ttctccattg ccggatccaa tgctttttga agaccaatca
     3901 gtccggcccc caacaaacct ggtccctctt gcgctcttct ttcgaattcc tcggtactta
     3961 tgtcatatgg cttactaaga cgcgataaac caaagatgcc gcatgcaaca atgagcacta
     4021 taactgctaa tccgatccag tcactcataa ttaagccagg caaacatatc ccggattgtt
     4081 gtcttcaaca atacccttaa ccttctcaaa gtctgcagtc gtgtcaacgc cagtcgaaaa
     4141 agtgcttgcc ttaattgcct taattttcac tccgttttcc atggcgcgta actgttcgag
     4201 cgcttccaat ttttcgagtt tagtctgctc ccacttcgta aactgaagta acacatcttt
     4261 ccgatagaca tataagcctg tgtgtttttt gaagagggcg agcagatcgg cctcacccgc
     4321 aagagctttt tcgagtgagc catatttcgt gactgcatcg cgaggaaatg gaatgggcga
     4381 acgagaaaag gataaagcgt aatcgcgttc gtctaagact attttgacca gatccggatt
     4441 cagcacgtca tccgccgatt cgattggttc ccagctagtg acgatcgaag ctccgttcgc
     4501 caactccatc tctcgctcaa tcgcacttac tgcgttttca attgtgattg gcgaaatgag
     4561 aggctcgtcg ccctggacat tgacgacaac ttcgcagtcc ggcagatgtg cagcgacctc
     4621 ggcaatgcga tcagtgccac ttttgtgtaa tgagctagtt aaaacgctgt cgaatccatg
     4681 cgcctgcgcc acctccataa tctttcgatc atcggtggca acgatgacat gattcacatt
     4741 tcgtgcggcc gtagcccgtt ccgcaaccca gcagatcatt ggttttccag cgattggaag
     4801 aagagccttt ccgggtaatc gggttgaacc gagacgggcg ggaatgacag caacaactct
     4861 gtttgactga ttgtttttgg gatttggccc cgcgtgattc acgggataac agattagctt
     4921 gcgaggcgga agactgtcaa tgaaagtcga ggaggaaggt ccttagggtc attggtcatt
     4981 gaagaaaatg gcaaaggact atttgataat tatgagttga gaattatgaa ttattaagtt
     5041 gtcatcgccg ccggtcttta aactctgcgt cactctgcgg tcctcggtgt cactctgcga
     5101 tgcttagtta aaaccaaaat gactacta
//
LOCUS       DASSFP010000322         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_825878, whole genome shotgun sequence.
ACCESSION   DASSFP010000322 DASSFP010000000
VERSION     DASSFP010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825878"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..676)
                     /locus_tag="VFH91_07160"
     CDS             complement(155..676)
                     /locus_tag="VFH91_07160"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET6862808.1"
                     /translation="MDHLKDLIREVPDFPKPGINFYDITTLLKNPEGLRATVDALAHE
                     FDGEEIDSVIGIEARGFIFAPALAYNLNAGFVPVRKPRKLPAECASISYDLEYGQDTL
                     EIHRDAVGDGHRVIIADDLLATGGTARAVIDLVERLGGVVIGLVFVVELEFLPGREKL
                     AGYDVRSLIKYSS"
     gene            complement(786..1064)
                     /locus_tag="VFH91_07165"
     CDS             complement(786..1064)
                     /locus_tag="VFH91_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET6862809.1"
                     /translation="MSKVARKFLVRGEVQGVGYRFFALRAAARHQVVGYVRNCPDGSV
                     EALVEGPANSVEAFKHDLATGPQWSVVTQLEELNIDPTGRYSSFRIEK"
     gene            1577..2980
                     /locus_tag="VFH91_07170"
     CDS             1577..2980
                     /locus_tag="VFH91_07170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6862810.1"
                     /translation="MKRLAIRFTVLTLLLTSWLPVTGAVTPAVTRAVTPAKPLNVPVV
                     YYKLPNGLKVVISEDHIAPVATVAVYYNVGFRLEPKGRTGFAHLFEHMMFQGSANVKK
                     FEHAKFIEANGGSLNGHTDFDYTNYYQTLPSNRVETALWLESDRMRSLDISEENLKNQ
                     QNVVSEEVRVNVLNQPYQFFEWIELWKNAFTNWNNSHNGYGELDEINAATIEDVRNFF
                     KTYYAPNNAVLTIVGDIDVADVKKMVDKHFGGIASQPMPARADLTEPAQTKEKRVSQT
                     DKLANLPALATGYHIPAQNSPDFPAMALLVQILQGDDSSRWYQRLVKEKELTLDLTGG
                     LNYFGNEFDYSGPMVMTTRTTYKPGHTADEVLREMDSVIAEVSSRGITEKELADAKIR
                     YRSNFYSQLESGYGKSHMLAVLALFRDDPSQINSLLTPFENVTAAQVKSAAAKYLVAS
                     NRTVIDRVPEAKTQTAQ"
     gene            3037..>3624
                     /locus_tag="VFH91_07175"
     CDS             3037..>3624
                     /locus_tag="VFH91_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET6862811.1"
                     /translation="MRILLSAVLLSIVTTVSLGQQKTAPQPGPARPLNLPRITEKKLT
                     NGLTVVLAPLPNVPKVSAILTFRAATNGTDRTAHPGIAQIAAGVANEGTDSRSSKQIK
                     EELRSIGGSLALGSDADSTTMTATALSEFSPRMFDLMSDVAQHASFPESEVKLAQENT
                     IQQIRAGRANPGFLVNERFQKAVFGNHPYGFVVPDE"
BASE COUNT          966 a          934 c          863 g          861 t
ORIGIN      
        1 cttgctcacc acagaggcac cgaggacaca aaggtttgca cggtgcaagc caagttaggt
       61 ctttggtcta tgggctttgg ccattggtgc gagttgctac gaatcgaacc gtcaaagatc
      121 aaaggccgaa ggccaaagcc caaagaccaa gccgctaaga agaatactta atcaaagacc
      181 tcacgtcgta gccggcgagc ttctcgcggc cgggcaggaa ttcgagttcg acaacgaaaa
      241 ccagaccgat aacgacgccg cctaatcgct caaccaaatc aataacagcg cgtgcggtgc
      301 ctccggtcgc gagtagatcg tcagcaatga tcacgcgatg gccgtctccg actgcgtctc
      361 gatgtatctc aagtgtgtcc tggccgtact cgaggtcgta agagattgaa gcgcactcag
      421 cgggaagttt ccgcggtttc cgcactggta caaacccggc attcaggttg taagcaagcg
      481 cgggggcaaa aataaatcct cgtgcttcaa ttccaataac ggaatcgatc tcttcgccgt
      541 caaactcatg agcgagagca tcgacagtgg ctcgtaatcc ctcagggttt ttcaacaaag
      601 tggtgatgtc gtaaaagttg atgccgggct taggaaagtc cggcacttcg cgaatcagat
      661 ctttgagatg atccatcttg attggtaggt gtcctttgta ttggggtgtg ttgctacgct
      721 cttcctgctt ttgctttagt taagcagagt caaagtacaa agatcaaagt acaaaataca
      781 agtagttatt tttcaattct gaaactcgag taccggccag ttgggtcgat attcagttcc
      841 tcgagttgcg tgacgactga ccattgaggg ccggtcgcca ggtcatgttt aaaagcttcg
      901 acgctgttgg ctggtccttc cacgagcgct tcaacactac catcaggaca gttgcgcacg
      961 taaccaacaa cctgatgacg cgcggcagct ctaagagcaa agaatcgata acctacacct
     1021 tgtacttctc ctcgcaccaa gaattttctg gctactttac tcataaactt ataagtgttg
     1081 attcccagcc tcaccagccc gagttagcag cgatgattac aggtccagta cagttgtttt
     1141 cacggtcccg cattgtagcg ttttttagtg acctacgcct cacgcaaggt cacgggactc
     1201 aagaatacaa tccacagatt acgccgatta cccagatttc ttcagagaaa ggcacggcgc
     1261 aaaggcgttc tagtaaagaa tctatgtaat cggcgtaatc tgtggattgt attcttgagt
     1321 tttcacacgc gctgaagccg gtggtgtctg cctctcacgg tctaactaaa ccaggaaacc
     1381 ttctcagctg tcatctcgta gtaaacccat cttcggatga gcagcgagcg ttagtgtgtt
     1441 caatcccgct cttatccaaa tctgcgtagc tcaaatcttg tttttcgaat agggatagtt
     1501 ttttgcatcc acgtgcggat ttcaccgccc gaaaatcttg caagcgatcc tgctttcaaa
     1561 gtgaggactc ctgattatga aacgactagc aattagattt actgtgctga cgcttctgtt
     1621 aacttcatgg ctaccagtta ccggagcagt tacaccagca gttacacgag cagttacacc
     1681 ggcaaagccg ctcaacgttc ctgttgttta ctacaaactt ccaaacggcc tgaaggttgt
     1741 catctcggaa gaccacatag cacccgtcgc caccgttgcc gtgtattaca acgtcgggtt
     1801 tcgtttggaa ccaaagggtc ggaccgggtt tgctcatctc ttcgagcaca tgatgtttca
     1861 aggctcagcg aacgtgaaaa aattcgaaca tgcgaaattt atcgaagcta atggcgggag
     1921 tctgaacgga catacggatt tcgactacac aaattactac cagacgttgc cgagtaatcg
     1981 agttgagact gctctctggc tcgagtctga tcgcatgcgt tcgttggaca tcagcgagga
     2041 aaacctgaag aaccaacaga acgtcgtcag cgaagaagtg agggtaaatg tacttaatca
     2101 gccttaccag tttttcgaat ggatcgaact ttggaaaaac gccttcacca actggaacaa
     2161 ctcacacaat ggctatggag aacttgacga gatcaacgca gcaacaatcg aggacgtacg
     2221 aaacttcttc aagacatact acgctcctaa caacgctgtg ctcacgattg ttggcgacat
     2281 tgatgttgcc gacgtcaaga agatggtcga caaacatttc ggaggtattg cctcgcaacc
     2341 aatgcctgct cgcgccgatc taactgaacc agcacaaacg aaagagaaaa gagtttctca
     2401 aacagacaag ctcgctaact tgcccgcgct tgccaccggc tatcacattc cggcacagaa
     2461 ctctcccgac tttcccgcga tggccctgtt ggttcaaata cttcagggag acgacagctc
     2521 acgttggtat cagcgtcttg taaaagaaaa ggagctgacg ctcgacctca ccggcggctt
     2581 gaattatttc ggaaacgaat tcgactacag tggtccaatg gtgatgacga cgcgtacaac
     2641 ctacaaaccg ggtcacacag ctgacgaggt attgcgggag atggactccg taatcgctga
     2701 agttagctca agaggcatca cggaaaaaga gctggctgac gctaaaattc gttaccgatc
     2761 aaacttctac agtcaattgg aatcgggtta cggcaaatca cacatgctgg cagtgctcgc
     2821 actattccgc gacgatccca gccagattaa ttcgttattg acgccatttg agaatgtcac
     2881 cgccgctcaa gtgaagtcgg cggcggctaa gtatctggtg gcgagtaatc ggaccgtcat
     2941 cgatcgtgtt cctgaagcaa aaactcaaac cgctcaataa cgctgattac gtgtgtgaga
     3001 cacaccctaa cgaatttcaa tatgaaaggc actgaaatga gaattctttt atctgccgtc
     3061 ttactgtcga tcgttactac tgtgagtttg ggccaacaaa aaactgcgcc gcagccagga
     3121 cctgcacgcc cattaaatct acccaggatt acggagaaga agctcaccaa tggtttaacg
     3181 gtcgttcttg cacccctacc aaacgtaccg aaggtctcag cgattcttac ttttcgtgcc
     3241 gctacaaatg gcaccgaccg caccgcgcat cctggcattg ctcaaattgc cgcgggcgtg
     3301 gccaacgaag gaaccgattc gagatccagc aagcagatta aggaagagtt gcgatcaatt
     3361 ggtggttcac tcgccctcgg cagcgatgcc gactccacga ccatgactgc taccgctctt
     3421 tctgaattct cgccgcgcat gtttgatctc atgagcgacg tagcccaaca cgcatcattt
     3481 cctgagagtg aagtaaagtt ggcccaggaa aatacaattc aacaaatccg ggccggccgt
     3541 gccaatcctg ggtttctggt caacgagaga tttcaaaagg ctgtcttcgg taatcacccc
     3601 tacggcttcg tagttccaga tgag
//
LOCUS       DASSFP010000323         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1418860, whole genome shotgun sequence.
ACCESSION   DASSFP010000323 DASSFP010000000
VERSION     DASSFP010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418860"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFH91_07180"
     CDS             <1..193
                     /locus_tag="VFH91_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862812.1"
                     /translation="TRGSGSIQSDLDTRGSLLRAGESTMVDVNATQGQMYWVWLSSVE
                     FADGSDWNNSQYGTFIQQY"
     gene            379..1947
                     /locus_tag="VFH91_07185"
     CDS             379..1947
                     /locus_tag="VFH91_07185"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HET6862813.1"
                     /translation="MELPLTPLEFARRTRKLYPNAEAVIDGSIRRTYEQFFDRCDRWS
                     SVLQSLGVAPGDRVAYISSNTPAMLESFYAIPQIGAICVPINYRLIASDFAYLINHSG
                     AKVVCAHSDYLEAVESIRSQLTGVESFVALEGKMEGWLDYEQLVASANTNYSRPSISE
                     SDLISINYTSGTTSRPKGVMITHRNAYLNVVGTLLHHPLAGNERYLWTLPMFHANGWT
                     FVWTVTAVGGAHICLRKVDPVFVFEEIKREKITSFCAAPTVLIGIANAPEHVRADAPR
                     GLRIVTAGAPPAAATIERVEGELGWEITQAYGLTETSPFITICESRPEHAKLPLAQRC
                     TLKACQGVELLTSGELRVVDEDGAEVPHDGQTLGEIVVRGNAVMKGYYLDDEATANVM
                     RGGWFHTGDAAVIHPNGYAEIRDRLKDVIISGGENISSVEVEGLLLRHPAIQEVAIVG
                     LPHELWGEAPHAFVVLKSASQVTEEDLRQFARDNLAHFKAPHGVTFVDELPKTATGKI
                     QKYVLRGQRGVIKQ"
     gene            2057..2518
                     /locus_tag="VFH91_07190"
     CDS             2057..2518
                     /locus_tag="VFH91_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET6862814.1"
                     /translation="MKDDSPAVQVAGFISKFEPSIAKIVRESRSVLRTQFPSANELVY
                     DNYNALAIGYSPSERTSEVVFSLAVYPRWVDLYFMHGRSLPDPNGLLEGNGKQGAFVR
                     LKDASTLKLPAVNRIISQALKHSSTGFPSTGKGKTIVKSISAKQRPRRPNS"
     gene            2515..2910
                     /locus_tag="VFH91_07195"
     CDS             2515..2910
                     /locus_tag="VFH91_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862815.1"
                     /translation="MKLFAILMFMLALAAGSSEPGQTDMTEHKSCKDHPKVIGSCFTV
                     YGRLSAYNGAPTLRIWKIGTRRMLGVSEQRFQEDGYINIPDEIRNRVNFETDLLGNFL
                     ICPFTRQRTGEMQLVCIESGKNLETRKRK"
     gene            2912..3559
                     /locus_tag="VFH91_07200"
     CDS             2912..3559
                     /locus_tag="VFH91_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862816.1"
                     /translation="MSRLAAVLLLLSTFMLSAQTQHEVRPPLKAASDLGQFDLDGSGE
                     WEIADGKLVLNKAGTPSGPIRRPAALAILKGQAFSEVEFESDLRSTAESNVPRRDLDL
                     VFAYQGSNRFYYAHLSAVTDNVHNGIFVVDNSDRRRIDDGKAMPRLTDQNWHHVRLIW
                     NGSTGRVTVYLDSLPQPVLSATDRSITSGRVGVGSFDDTGEFRNIQVKGLRAEPR"
     gene            3569..4054
                     /locus_tag="VFH91_07205"
     CDS             3569..4054
                     /locus_tag="VFH91_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6862817.1"
                     /translation="MRKPEAITIVSEDPTTEDPLRLTRALTVELSDMYQDDGGANSFD
                     PNDAIGVRSIFVVARLSGEAIGCGAIRPLDVETAEVKRMYVVPHARRMGIGARILDEL
                     ESAARNMGYQRLRLETGLRQPEAIALYEAAGFAKVNCYGNYTANPMSVCFEKELDRQL
                     N"
     gene            4145..4384
                     /locus_tag="VFH91_07210"
     CDS             4145..4384
                     /locus_tag="VFH91_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862818.1"
                     /translation="MRKLFQICVMTLVVLFSLPFFLLAILLFIVLFNNRSTGTSGVAT
                     VTGGVSLPAWFFLILAFPVLSAGLYLITRWARHRR"
     gene            4420..>4720
                     /locus_tag="VFH91_07215"
     CDS             4420..>4720
                     /locus_tag="VFH91_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862819.1"
                     /translation="MAEGSTRRNFLQAGLSFLGLTTLERLNLGHLQDTEKTFDDIRAN
                     LLQMINEERAIDKLRPVVLDEFATWVATQHAREMAVNDFASHWGMDGLKPYHRYSF"
BASE COUNT         1134 a         1156 c         1231 g         1199 t
ORIGIN      
        1 gacacgcggt agtggatcga tccagtcaga tttagatacc cgcgggtcgt tgcttcgtgc
       61 cggtgaatcg acaatggttg acgttaacgc aacgcaaggc caaatgtact gggtgtggct
      121 ttcgtcggta gagtttgccg acgggagtga ctggaacaat tcacaatacg gcacgttcat
      181 tcaacagtat tagggttgac acccagtttt agtgaatccg ccaaccgcac agcggatgac
      241 ttaccggtcg tgcctcgccc gctagtcagt gctgtccatc ttaccagttc atcacaatac
      301 tcaatttttt ttgacgtcta cacaattgaa tggtagaact cctcggcgtg aatagataac
      361 ttaggcaggt acgtcaaagt ggaattgccg ctaactcccc ttgagtttgc ccggcgaaca
      421 cgcaaactct acccaaacgc tgaggccgtt attgacggtt caatacggcg aacctatgaa
      481 cagttttttg accgctgtga tcgatggtcc agcgtgctgc aatcgcttgg tgtggctccc
      541 ggtgatcggg ttgcgtacat ctcctccaac actcctgcga tgcttgagtc gttctatgcc
      601 attcctcaga ttggcgccat ctgcgttccg atcaactatc ggttgattgc cagcgacttt
      661 gcttacttga tcaatcacag tggcgccaaa gtggtttgtg cgcactctga ctaccttgaa
      721 gcagtcgagt cgatccgctc gcagttgacc ggggtcgagt ccttcgtagc gcttgaagga
      781 aagatggaag gatggttgga ctacgagcag ttagtcgcca gtgcaaacac caattattcc
      841 cgtccaagca tctctgagtc agacttaatc tcgatcaatt acacgagtgg aacaacgtct
      901 agaccgaaag gtgtgatgat tactcatcgc aatgcctatc tcaatgtggt tggtacccta
      961 ctccatcacc cacttgctgg taacgagcga tacctttgga ctcttccgat gtttcatgca
     1021 aacggttgga cctttgtgtg gactgtaaca gccgtaggcg gcgcgcacat ttgtttgcgc
     1081 aaagttgacc ctgtttttgt tttcgaagaa ataaagcgcg aaaagattac aagcttctgt
     1141 gccgcgccaa cggtcttgat aggtattgcg aatgccccgg agcacgttcg agcggacgca
     1201 ccgcgaggtc tgcggatcgt cactgcgggt gcaccgccag ccgcggcaac catcgaacga
     1261 gtcgaaggtg agttgggttg ggaaattacc caggcttatg gtctaaccga aacttctccg
     1321 ttcataacaa tctgtgagag tcggccggag cacgctaaat tgccgttggc ccagcgttgt
     1381 actcttaaag catgtcaggg ggtggaattg ttgacctctg gagagctgcg ggtcgtagat
     1441 gaggacggag cggaggtccc gcatgatggt caaactctgg gcgagattgt agtgcgaggt
     1501 aatgcagtga tgaagggtta ttaccttgac gacgaagcga cggcgaatgt gatgcgtggg
     1561 ggttggttcc acacaggaga tgccgcggtt attcatccga acggatatgc tgaaattcgt
     1621 gaccgcctca aagatgtgat catcagtggc ggcgaaaata tctcttccgt tgaagttgaa
     1681 ggtctgctgt taagacaccc tgctattcaa gaagtagcaa tcgtcggtct accacacgag
     1741 ctctggggag aagcaccgca tgcatttgtt gttttgaaat cagcctctca ggttaccgaa
     1801 gaggacctac gacagttcgc tcgcgataac cttgctcact ttaaggcgcc gcacggcgta
     1861 acctttgtcg atgagcttcc gaagacggct acgggtaaga ttcaaaagta cgttttgcgc
     1921 ggccaacgcg gggttatcaa acagtaggac caactgttgg ggcgtccctc cgtgggcgcc
     1981 ctctcatggt ggcagctttc gcattgataa gggcgcatac ggagggcgcc acgacagata
     2041 acgttcctaa catacgatga aagacgattc tccagccgtc caggtggcag gattcatttc
     2101 caaatttgaa ccgtcgatcg ccaaaattgt gcgcgaaagt agatctgtac ttcgaaccca
     2161 attcccatcc gcaaacgaac tcgtctacga taactacaat gcgctagcca ttggctactc
     2221 acccagcgaa cggacctccg aagttgtttt ctcacttgca gtttatccac gctgggtcga
     2281 tctttatttc atgcatggca gatcgctgcc tgatcccaat gggttgttgg agggcaatgg
     2341 caaacaagga gcattcgtga gactgaaaga tgcaagtacg ctcaagctgc cggcggtcaa
     2401 caggataatc tctcaggcac tgaaacactc ttcaaccgga tttccgtcga ctggcaaggg
     2461 aaagactatt gtgaagtcga tctcggcgaa gcaacgcccg agaagaccaa actcatgaaa
     2521 ctgtttgcaa ttctcatgtt tatgctcgcg ctggctgctg gttcatcaga gccaggacag
     2581 acagatatga ccgaacacaa gtcatgtaag gaccatccaa aggtgatcgg ttcatgtttc
     2641 accgtttatg gaaggctatc cgcttacaac ggagcgccaa ccctgcgtat ttggaaaatt
     2701 ggcactcgcc gcatgctcgg cgtttcggag caacgatttc aagaggacgg gtacattaat
     2761 attccggacg aaattcgtaa ccgagttaat ttcgagaccg acttgcttgg aaacttttta
     2821 atttgtcctt ttactcggca gcggacagga gaaatgcaat tggtttgcat tgagagtggt
     2881 aagaacctgg aaacacgcaa acgaaaatag tatgagtcga cttgccgctg tgctgttact
     2941 cctttcaacc tttatgttga gtgctcaaac tcaacatgaa gttcgacctc ctttgaaggc
     3001 cgcttcggat ctcggccaat ttgatctcga tggttcggga gaatgggaaa ttgctgacgg
     3061 gaaattagta ctgaataaag ctggaactcc atccgggcca attcgccggc cggcggcgct
     3121 agctatttta aagggacaag cgttttcaga agttgagttc gagtctgacc tcagatcgac
     3181 ggctgaatca aatgttccgc gtcgagattt ggatctggtc tttgcttatc agggatctaa
     3241 ccgtttctac tacgcccatc tttctgctgt aacagataac gtccacaatg gaatctttgt
     3301 ggttgacaac tcggatcgcc gacgaattga cgacggcaag gcgatgcctc gactcacgga
     3361 tcaaaattgg caccatgtac gactgatttg gaatggctcg accggtcgtg tgactgtcta
     3421 cttagattca ttgccacagc cggttctttc agcgacggat cgctccatca cttcaggacg
     3481 tgtcggcgtt ggttcgtttg atgacacggg agagtttcgg aacattcagg tgaaaggttt
     3541 gcgagccgaa ccgcggtaac attttcccgt gcgcaaaccc gaagccataa caatcgtctc
     3601 cgaagatccg acaactgaag acccgcttcg ccttaccaga gcgcttaccg tcgagttaag
     3661 cgacatgtat caggacgatg gtggagccaa ctcgtttgat cccaatgatg cgattggcgt
     3721 ccgttcaatt tttgttgtgg cgcgacttag tggagaagca atcggctgtg gtgcgataag
     3781 acctctggac gtcgaaacag cagaagtgaa gagaatgtat gtggtgccgc atgctcgtcg
     3841 aatgggtatt ggcgcgcgca ttcttgatga gttggagtcg gcggccagaa atatgggata
     3901 tcagcgtctg cgattggaga ctggtctaag acaacctgaa gcaattgccc tctatgaggc
     3961 cgcgggattt gcaaaggtga actgttatgg taattacaca gccaacccga tgagcgtctg
     4021 ttttgaaaaa gaactggata gacaactcaa ttaaataaca aacgtcagca aggccgcctt
     4081 ctatgcgcaa ccctgtgttc acctgatata taacaaagcg ccgccgggcc ggcgcactcc
     4141 aaggatgaga aagctttttc agatttgtgt catgacgctc gtagtgcttt tctctctgcc
     4201 cttttttctc ctggcaatat tgttattcat agttctcttt aataatcgtt cgacagggac
     4261 ttctggagtg gccacggtca ccggtggcgt tagcctaccg gcgtggttct tcctcatttt
     4321 agcttttcca gttctgtctg ccggtctcta tctcattact cgttgggcca ggcatcgtcg
     4381 ttgatttaga agccgtttca cctcattcgc tcggttccta tggctgaagg ctccactaga
     4441 agaaactttt tacaagctgg tctttccttc ctgggcctaa caaccctgga aagactcaac
     4501 ctcggccatc ttcaggacac cgagaagaca ttcgatgaca tacgcgcgaa ccttttgcag
     4561 atgatcaatg aggaaagggc tatcgataag ttgcgtcccg ttgttctcga tgaatttgcg
     4621 acttgggtcg ctactcaaca tgcccgcgaa atggcggtta acgacttcgc gagccactgg
     4681 ggaatggatg gtcttaagcc atatcaccgc tattcatttg
//
LOCUS       DASSFP010000324         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_236505, whole genome shotgun sequence.
ACCESSION   DASSFP010000324 DASSFP010000000
VERSION     DASSFP010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236505"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1850)
                     /locus_tag="VFH91_07220"
     CDS             complement(333..1850)
                     /locus_tag="VFH91_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862820.1"
                     /translation="MADLLIVDDELGMRQFLTHLFQREGHTVRVAENGKAAMELLREK
                     PADLVLSDIRMPDMGGIDLLRAAREFNPDSEVIMMTAFANVDTAREAFLLGAYDFVQK
                     PFDNALLKETVARALKKISLIKEKEALLEENKTLIQGQRTRGKLGNIIGRSQRMLSLY
                     QMIETVAQVPSTVLVTGESGTGKELVARAIHDMGPRAEKPFVSVNCGAFTETLLESEL
                     FGYVKGSFTGANANRKGLFEAANSGTIFLDEIGEMSPAMQVKLLRVLQERKVRPVGAT
                     EETLVDTRVIAATNRDLARMVSEGTFREDLYYRISVIPIELPPLRERSEDIADLASHF
                     AEKFCGPAGRNLTVSDKAMVLLERYSWPGNVRELEHTIERAVALETADSIQPERLPDK
                     VTNYNPYRVAEAMEFPDDGLYLTAHLDQLEKTYIVEALRRTSGNQTNAAELLRLSVRS
                     LRHLLDKHGIRGLTAQMRDERRSPESTPRRRATDPTPRRREDDEAEFAAGAGDGT"
     gene            complement(1843..3558)
                     /locus_tag="VFH91_07225"
     CDS             complement(1843..3558)
                     /locus_tag="VFH91_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862821.1"
                     /translation="MQGAKYDSLERDMPSSDKGNSTDKGASSRLGWLILGRLFTALLL
                     FILGLVWTRESFPRQIPNRGVILLATVAGLTILYSIVLRLSRNISLQAKGQIAIDVLL
                     VTWLVWASDVVHSPYIALYILVIASSGVFLGSRDALVTSVGCAVLFTASASAVIFGQD
                     QRALIDGSVAQTVQSVGLFDVSFLVVGLLSARLSERQSRSDVRLLAATQSLANLRALH
                     ERIVESIRSGLVTTDLDGHIFTFNAAAQEITGFSEESIRGQDASILFGNMKEHIAVTL
                     EAARTEATVPRFEADCLTHDGVRLRLGFGVSPLFSETGETTGMVITFQDLTQVRTLEE
                     TSRRQDRLAAIGRMAASIAHEIRNPLAAMRGSIQMLRDEVENDSSQAELMEIILRESD
                     RLNRIITDFLNYARPRSLTPSRVNVGDLLHQTFTLLRHSPEIGADQQVNEELPDEPLF
                     AEADEGQLKQVFWNLARNALQAMPDGGVLRASLEKNSHNRIRISFSDTGRGMSPDQVE
                     RLFEPFSSTTGGTGLGLSIVYQIIRDHGGTINVRSREGHGTTIMVELPFATSDNNSNS
                     EINHG"
BASE COUNT          914 a          985 c          996 g          925 t
ORIGIN      
        1 taataccgat aatcgcgaca acgattaaaa gttcgatcaa cgaaaagcct tcttggcgtt
       61 tggtagtcat tctgatttcg tgctccttaa gagaaattag gggttcaact gtttctcgcg
      121 ccaccggcct tcgttcacgt agtgatgccg cgcagagcgc gaagtgtccg cgagtactaa
      181 aacaaccact gtgccagtgt cggaaatgtc gaaaatggca ggaaaatcaa agatttggcg
      241 ggaccactga acccttgtga ggtgagcctg acattttgcg gcagcgaggt gctgacgctt
      301 tgtggcagat aacgtgggcc taacgtgaaa tgttaggtcc cgtcaccggc tccggcggca
      361 aactccgctt cgtcatcttc gcgccgtctg ggagttggat ccgtggcccg acgccgaggg
      421 gtcgactcgg gactacggcg ttcgtcacgc atctgagcgg tgagccctct gataccgtgt
      481 ttatcgagca gatgccgtaa tgacctcacg gagagcctta gcaattcggc agcattggtt
      541 tgattgccgg aggtgcgccg tagcgcttcg actatgtaag tcttttccag ttggtccaag
      601 tgtgcggtca agtagagacc gtcgtccggg aactccatcg cttcagcgac gcggtagggg
      661 ttgtagtttg tgaccttgtc cggcaaccgt tcgggttgaa tcgagtcggc tgtctcgaga
      721 gcgacggctc gctcgatggt gtgttccagt tcgcggacat tccccggcca tgaatagcgt
      781 tccaggagga ccatggcctt gtcgctaaca gttaagttac gacccgcagg accacagaat
      841 ttctcggcga agtgggaggc caaatctgcg atgtcttccg agcgttctcg cagtggaggc
      901 agctcaatgg ggatcacgga aatacggtag taaagatcct cgcgaaatgt tccctcggag
      961 accatgcgag ccaaatctcg gttcgtagcg gcaatgactc tggtatctac aagcgtctct
     1021 tcggtagctc caaccgggcg aaccttgcgc tcctgcaaaa ctctcagaag cttaacctgc
     1081 atggcaggag acatttcgcc tatctcatcc aggaaaatag ttccactatt tgccgcttcg
     1141 aagagtcctt tcctgttggc atttgcgcct gtaaatgagc ctttaacgta cccgaacaac
     1201 tcggactcta ataatgtttc ggtgaacgcc ccgcagttaa cggacacaaa gggcttttcg
     1261 gcacgtgggc ccatgtcatg tatcgcgcgc gctaccagct cttttccggt cccggactct
     1321 cccgtgacca gtacggtcga tggaacctgg gctacggtct ctatcatctg gtaaagcgac
     1381 agcattcgtt ggctgcgtcc gatgatgttt ccgagtttgc ctctggtcct ttgaccctga
     1441 atcagagtct tgttttcttc gagcagtgct tctttttctt taattagcga aatctttttg
     1501 agtgcgcgcg cgacagtctc tttcaaaaga gcattgtcga aaggcttctg tacaaaatcg
     1561 taagctccga gaaggaatgc ttcacgcgcg gtatcgacgt tagcgaaagc cgtcatcatt
     1621 attacttcac tatcaggatt gaactcgcga gctgcacgca aaaggtcaat tccgcccatg
     1681 tcgggcatgc gtatgtccga aagtacgaga tcggccggct tctctcgcag taattccatt
     1741 gcagctttgc cgttctctgc gacacgtacc gtgtgtcctt cacgttgaaa aaggtgtgtc
     1801 aggaattggc gcattcctaa ctcgtcatca actataagca agtcagccat gatttatttc
     1861 actattcgaa ttattgtctg aggtggcgaa gggtaattca accatgatgg tcgttccatg
     1921 tccttcgcga ctacgcacgt tgattgtacc accatgatct cgaatgattt gataaacaat
     1981 cgaaagtccc agccctgttc caccagttgt tgaggaaaaa ggttcaaata agcgctcgac
     2041 ctggtcgggc gacatacccc gtccagtgtc agaaaaagag atgcggattc gattatgtga
     2101 atttttttca agacttgccc gcaaaacacc gccgtccggc attgcctgca gagcattccg
     2161 cgccaggttc cagaagacct gcttcagttg tccttcatcc gcctccgcaa agagcggctc
     2221 gtctggaagc tcttcattaa cctgctgatc ggcacctatc tccggactat ggcgcaataa
     2281 agtaaaggtt tgatggagca gatcgccaac gtttacacga gatggagtta gcgatcgtgg
     2341 acgggcgtaa ttaaggaaat ccgtaatgat tcgattcaga cgatcagatt ctcgaaggat
     2401 aatttccatc aattccgcct gagaagagtc gttctcaacc tcgtcgcgca gcatctgaat
     2461 cgaaccacgc atggctgcca gcggatttcg gatttcgtgc gcgatcgacg cagccattct
     2521 gccgatcgct gccagccggt cctgacgccg ggaggtttct tccaacgttc ttacttgagt
     2581 taagtcctgg aaggtaataa ccatgccagt ggtctcacct gtctctgaaa agagaggtga
     2641 aacgccaaaa cccaaccgaa gccggactcc atcgtgagta agacaatccg cttcaaaacg
     2701 cggcaccgtt gcttccgttc tcgctgcttc aagcgtaacg gcgatatgct ccttcatatt
     2761 gccgaagaga attgaggcgt cctggccgcg aatactctct tccgaaaagc ctgtaatttc
     2821 ttgcgcggct gcattgaacg taaagatgtg accgtccaga tctgttgtga ctagaccaga
     2881 gcgaatcgac tcgacgattc gttcatgtag tgctcgcagg ttagcaaggg actgtgttgc
     2941 cgcgagcagc cttacatcag acctggattg gcgttcggat aagcgcgctg agaggagccc
     3001 aaccacgagg aaagaaacgt cgaagagacc gactgactga acagtttgag cgaccgaccc
     3061 atcgatgagt gccctttggt cctgtccgaa gatcacggcg gaagcagaag cggtaaataa
     3121 tactgcgcag ccaaccgagg tcacgagagc gtcacgcgaa cccagaaaca cgcctgagct
     3181 cgcgatcacc aaaatgtata atgctatgta gggagaatga acgacatctg aggcccacac
     3241 cagccaggta accaacagga catcgattgc aatctggcct tttgcctgaa gagaaatatt
     3301 gcgcgagagt cgtaatacga tcgagtaaag aatcgtcagg ccagcaaccg ttgccagtag
     3361 aatgacgcct ctatttggga tttgacgagg aaaggactcg cgtgtccata cgagcccgag
     3421 gataaatagg agaagtgccg taaagagtcg accgaggatg agccatccca gcctcgagct
     3481 cgcaccttta tcagttgagt tgcccttgtc tgaggacggc atgtcgcgct caagactatc
     3541 atactttgca ccttgcattt ggtactttgt gcttagtact ttgtcgtcgg tattttgata
     3601 ggcaaaagct cttaagtggt ttggcgctca agttcactcg aaaagaggta gtggttcatt
     3661 tcaatttctc tgcgtttctc tgcgtgatct ccgcgttctc tgcgtcttgg ctgtgtagct
     3721 ctataaggat ctccgacaag acgcagagaa cgcggaggtc acgcagggaa cgcaaagaac
     3781 agcaaactga accaatactc cgaaagacaa gaacaccctc
//
LOCUS       DASSFP010000325         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1577606, whole genome shotgun sequence.
ACCESSION   DASSFP010000325 DASSFP010000000
VERSION     DASSFP010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577606"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1909
                     /locus_tag="VFH91_07230"
     CDS             215..1909
                     /locus_tag="VFH91_07230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET6862822.1"
                     /translation="MSSVLEIIPLGGIGEFGMNCMALRYGDEMLILDAGMGFPEETAY
                     GVDVSIPNFNFLEEYRDDITAIVLTHGHEDHIGALPYILKRFNVPVYCSHFTAGLAES
                     KLEEHDLIGDVLLHRVEPRDVVDIGSFSIEFIRVSHSLVDCFSLAIKTPVGTIIHTGD
                     YKVDETPVIGEPIDLRSFRRYGQDGVLALLSDSTNATVPGRTPSERAVIPAFEEIFSE
                     STGRIVVAAFASSIHRLQIVLDVAQQFNRRVCVLGRSMQKNVEVADRLGYLDIPDGLL
                     VSLNDAKQMSDDAVVFLVTGSQGEARAALSQMATQSYKGLMIEEGDTVVLSARIIPGN
                     ERLISRMIGFIYKRGANIIEEKRRLVHVSGHASQEDIRIMTEAVRPKFVVPVHGEYRM
                     LFRHKEFVKNHLGYAEENIILIENGDVLELDGERAAVIDKREIGRTFIDETGFEEIES
                     DTVRERKQLAYEGTVTAVITLDAETGELLEDPRIVARGVRGLSTNGSINTDPEQSNST
                     LLDDAKRVLTSATVGASRQTLEDDSLFKEHLRVELKRFIQKQTGSRPVITPVVVRV"
     gene            1948..2697
                     /locus_tag="VFH91_07235"
     CDS             1948..2697
                     /locus_tag="VFH91_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6862823.1"
                     /translation="MVLLTGASSGIGKEIALQLAQKGAKLGLLARRQDVLDTLVEQAQ
                     KQQTKAIALPADVRDAAAVRAAADRLRLEFGPIDVMIANAGIGTASHATKFKPEQVAE
                     VININLIGAANSVAAVLPEMQTRDAGHLVAISSLSAYRGLPKSAAYCASKAGLSSFFE
                     SVRVDLRDTRIKVTIIHPGFIRTPLTAGREAKMPFLMEVDEASRKILGAIEQGKKSYA
                     FPWQLASVVRAGMIMPNFMYDWIAARSSFRE"
BASE COUNT          709 a          704 c          769 g          699 t
ORIGIN      
        1 tggtgggaaa gtatagcacg gagaactttg gagtgcgtcg gcccggcggc gcgttgttat
       61 ataagtaagg ccggggcggg tcgacggatt ttaaaagcgc cgccgggccg acgcactcca
      121 aagttctccg tgctatactt tcccaccatt aacctcaaga tcctggtgtc atcggtcaag
      181 aggccttctc cggagagtag agtaataaca aaaagtgagt agcgtccttg agatcattcc
      241 gctcggcggc attggcgagt ttgggatgaa ctgtatggcc ttgcgctatg gcgacgagat
      301 gttgattctc gatgcgggca tgggcttccc tgaggagact gcttacggcg tagacgtttc
      361 cattccaaat ttcaattttc tggaagagta tcgcgacgat atcacagcga tcgttctgac
      421 tcacggtcac gaagatcaca tcggtgcgct accttacatc ctgaagagat ttaatgtgcc
      481 cgtttactgt tctcacttca cagcgggttt ggccgaaagc aagcttgaag aacacgacct
      541 gatcggtgat gttctacttc atcgtgtcga acctcgcgat gtcgttgaca ttggttcgtt
      601 ttcaattgag ttcatcaggg tctcgcattc gcttgtcgat tgcttctcac ttgcgatcaa
      661 gactcccgtt ggcacgatca tccataccgg ggattacaaa gttgacgaaa caccggtaat
      721 cggagaaccg attgacctga gatcttttag aagatatgga caagatggag tgctggctct
      781 tctttccgac tcaaccaacg ccaccgttcc aggtcgcact ccttcggagc gtgctgttat
      841 tccggcattc gaggagattt tcagcgagtc gacaggcaga atcgtcgtgg ccgccttcgc
      901 gtcgtctatt caccgtctgc aaatcgtttt agatgtcgca caacaattta atcgccgggt
      961 ttgcgtgctc ggccgctcta tgcagaagaa tgtcgaagtc gccgaccgtt taggctatct
     1021 cgatatacca gatggccttc tggtatcgct caacgacgcg aagcaaatga gtgatgatgc
     1081 ggtcgttttt ctcgtcacag gttctcaggg tgaagctcgc gcagctctgt cgcaaatggc
     1141 gactcagagt tacaaggggc tcatgatcga ggagggtgac acagtagtct tatcggcccg
     1201 gatcatcccc ggcaacgagc gcctcatttc acgaatgata gggttcatct acaagcgtgg
     1261 cgcaaacatc attgaggaaa agcgacggct ggtccacgtc agcggacatg catctcagga
     1321 agatatccgc atcatgacgg aagccgtgcg gccaaaattc gtcgttcctg tgcacggtga
     1381 atacagaatg ttattcaggc ataaggaatt cgtgaaaaac catcttggtt atgcggaaga
     1441 aaatatcatt ctgattgaga atggtgacgt gttggaattg gatggcgaga gagctgcagt
     1501 catcgacaaa cgcgagattg gccggacctt tatcgatgaa acagggttcg aagagattga
     1561 gagcgacaca gttcgagagc gcaaacaatt ggcttacgaa ggaactgtga cagcagttat
     1621 tacgcttgac gcagaaactg gagaactttt ggaagatccg cgaatcgtgg cgaggggtgt
     1681 aagaggtttg agtaccaatg gttctattaa cacggatcca gaacagtcca actcaacact
     1741 cctcgatgat gctaaacgtg ttctcacctc tgcaaccgtt ggtgcttccc ggcaaaccct
     1801 ggaagatgac tcgctcttta aagagcatct ccgagtcgag ttaaagcgtt tcattcagaa
     1861 acaaactggt tcccgccccg tgatcactcc tgtagttgta cgagtctgag agagcgacat
     1921 acaacgttga cctattggac agacaaagtg gtgctcctta ccggcgcttc gtcaggcatt
     1981 ggcaaagaga tcgcccttca gctggcccaa aaaggcgcta agttaggctt gctggcgcgt
     2041 cgtcaggatg tgctggatac cctggtcgaa caggcccaaa aacaacaaac aaaagcgatt
     2101 gcccttcccg cggatgtgcg agatgcggca gctgtacgcg cggcggcaga tcgtctgcgc
     2161 cttgagttcg gaccaattga cgtgatgata gcgaacgctg gaatcgggac cgctagccac
     2221 gcgacgaaat tcaaacccga acaagtggca gaggttatca acattaatct tattggagcc
     2281 gctaacagcg tcgcggctgt acttcctgaa atgcaaacaa gagacgccgg acacctggtc
     2341 gccatctcca gtctgtccgc ttacagaggg ctgcctaagt cggcggccta ttgtgccagc
     2401 aaagcgggac tttcgtcctt ttttgagagt gttagagttg acctgcgcga cacgcgaatc
     2461 aaagtgacaa taattcatcc cggcttcatc cggacgcccc tgaccgccgg tcgtgaagcg
     2521 aagatgccat ttttgatgga agtggatgag gcaagtagaa aaattcttgg agcgatcgag
     2581 caagggaaaa agagttacgc gtttccgtgg cagctcgctt ccgtggttag ggcgggaatg
     2641 ataatgccca actttatgta cgactggatt gccgcacgca gttcatttcg tgaataactt
     2701 tattcgagct cgcgcctttt taacattaac ccagagcgac aaacctaaga tcaaataata
     2761 atgtccatct tacaagcgat actacttgga ttagtccagg gtctgactga gtttattcca
     2821 gttagctcca cggcccacct tgtttttgct gcccgacttc tcaatctgta ttcgggggct
     2881 g
//
LOCUS       DASSFP010000326         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1717261, whole genome shotgun sequence.
ACCESSION   DASSFP010000326 DASSFP010000000
VERSION     DASSFP010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717261"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFH91_07240"
     CDS             complement(<1..855)
                     /locus_tag="VFH91_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862824.1"
                     /translation="MSSIKLNLVDSQNTLSGTIHASIADFCVAALAAEPETLNELEAA
                     LTRFQKDPIEIRSRLRLHDGLVVDTEPYDAGLIIVDLAARIVVCDSTYSLPGPSGVVE
                     YDDGLHCLDCPIKYQLNPDEWLFLDSVNGFFSHHDSRQQTRALNPPLDARPVLYGRAL
                     VEFIAQRILCVIEETNGRSPSASPDTEPIDSIRQTITPTSELILDEHRHELENADEPD
                     PFSRHISAIHKEWLMTPRDDLSGQSPREVMLAKQDLINFDLDSRSLQWSLLLEGPPCL
                     DRNSFAYRF"
     gene            1103..3478
                     /locus_tag="VFH91_07245"
     CDS             1103..3478
                     /locus_tag="VFH91_07245"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET6862825.1"
                     /translation="MIPQSIPIARQPFGDRAFRTALLTAIIVFVTYYLTAKIGFEFAL
                     QPGSVSTLWMPNSLLLAALLMIERRWWWLIVAAALPAHLASEFQSGVPATMVFCWFVS
                     NSVQALLAAFCITYFIKEGTVYFERIRDLSLFLLFGAFLAPFAASFLDSALVQLNNWD
                     HRPYWEIWRVRFLSNVLASLTLIPFVLVWFRGGLGIILNAPLRRYLEAALLAAGFLVV
                     ALYVFNTQRGFAEKTPSLLYWPLPFLLWSTVRFGLRGVTTSLVFIMFVAIDGATRGAG
                     PFVTSSALDNAMSIQMFLIVVSIPLMVLASIIEERRKVEAAARENEERLMLALNAAQM
                     ETWDWRITDNRLIWPDETRNGFGVKSKEDISPETFFRLVHPEDREKVERATSSAVREG
                     VPYEIEYRMLADGETRWFLSKGTVLRDELGRPARMLGIGIDITERKTAEEALEKINDR
                     NQAILRAVPDMMFLLTRDGVYLDYSAREVDKLLVPPSDFIGKSIKEVMPRDLAESFIK
                     ILSELSATDEPYVVEYTLPMNGETKFFEARLILAEGENVLTIVRDVTEAHQAIDSARE
                     SQEKVLQSNKQIRALAARLISAQESERRRISLLLHDDVSQNVAALGLSISRLKRKLPT
                     SNDELLDELGQLGSQAYDLTTQIRRLSHQLHPEVLEHLGLVKALESHVTEFGYEEHIE
                     TSFKADVRSEPLPIDLSVCLYRVALEGLRNISRHSGAKSAEVILRETNGCLMLQVSDS
                     GFGFDVERARRGNGIGLASSEERIRLLQGSFEIHSSPETGTIMTARVPLMR"
     gene            3481..4116
                     /locus_tag="VFH91_07250"
     CDS             3481..4116
                     /locus_tag="VFH91_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6862826.1"
                     /translation="MDRTRILIADDHKMFVQGLQGLLEDEFDLVGTVGDGHALVEEAV
                     RLDPDVILVDISMPSLNGLDAVRRLKTKGLNACIIFLTMHADDRLLAEAFRCGGKGYV
                     LKQSAGEELILAIKQVLAGHKYVTPLIATEWVQDLTEQIGEKQKLTLTPRQREVLQLV
                     IEGCTMKEVATRLGISTRTAESHKYEMMEGLGVESTAELIQYAIKLGLTRQ"
BASE COUNT         1048 a          980 c         1093 g         1083 t
ORIGIN      
        1 aaatcggtat gcaaaagagt tgcggtccaa acacggaggc ccttccaaca acaaggacca
       61 ctgcaagcta cgtgagtcaa ggtcgaaatt aatcaagtct tgtttggcaa gcattacttc
      121 gcgaggtgat tgaccactta aatcatcccg cggcgtcatc agccactcct tgtgaattgc
      181 actgatgtgt cttgaaaatg gatcaggctc atcggcattt tccaattcat ggcgatgttc
      241 atccaggatt aattcgctag ttggtgtaat cgtctgccta attgaatcaa taggttcggt
      301 atctggtgaa gctgaaggcg atcttccatt agtttcttca attacgcaca agattcgttg
      361 agcgatgaac tcgacaagcg ctcgaccata aaggaccggg cgcgcatcaa gcggagggtt
      421 gagagctctc gtctgctgcc tcgaatcatg gtggctaaag aagccattga ccgagtcgag
      481 gaagagccac tcatctggat tcagctggta tttgatcggg caatctaaac agtgtaaacc
      541 atcgtcatat tcgaccactc ccgacgggcc gggcagagag tacgttgagt cgcagaccac
      601 gattcgtgct gctaggtcta caattatgag gcccgcgtca taaggctcgg tgtccactac
      661 aaggccgtcg tgaagtcgca agcgcgaccg gatctctatc ggatcctttt gaaagcgtgt
      721 cagggccgcc tcgagctcat tgagcgtttc tggttcagcg gcaagagctg ctacgcaaaa
      781 atcggcaatc gaagcgtgaa tcgtgccgga aagtgtgttt tgagagtcta cgagattgag
      841 ttttatgctg gacatgtgag gttcctttca attgtgagtt taaaacggcc gcattttcgc
      901 ctaagactgt aaaaaatgcc agctgtaggc tgttataaat ttgatcatac ttactattta
      961 aagacgttct ggttcccact tcccaaatgg aaaattgaga aatggaaaat gaccgatgaa
     1021 aatcaacgcc tcctcttgtt ctattcctca tgaccccctc ataatcaacc agaacaattt
     1081 cacctgatcc ggagaaatct tcgtgattcc ccaaagcatt ccaatagcca gacagccttt
     1141 tggtgaccgc gccttccgga ctgctttact gaccgcaatc atcgtcttcg tcacctacta
     1201 tctcaccgcc aaaataggct tcgagttcgc actgcaacca ggttcagtat cgactctctg
     1261 gatgccgaac tcgctgttgc ttgcggcgct cttgatgatt gaaaggcgtt ggtggtggct
     1321 gattgttgcg gccgccttgc cggctcacct tgcgtctgag tttcaaagcg gggtgccggc
     1381 gactatggtt ttttgctggt tcgtatcgaa ctccgtgcaa gctctgctgg ccgcattttg
     1441 tatcacctat ttcataaaag aagggactgt ttatttcgaa aggattcgcg atctaagtct
     1501 ctttttactc tttggcgcat tccttgcacc cttcgctgcc tcttttctgg acagtgctct
     1561 ggtccagcta aataactggg accatcgtcc atactgggag atttggcgtg ttcgcttctt
     1621 gtccaacgtc cttgcaagtc taacgctcat tccttttgta ctggtctggt ttcgtggtgg
     1681 cctgggaata atcctcaacg cgccgttgag acgttacctc gaagccgctc tgctcgctgc
     1741 aggatttctc gtcgtcgctc tttatgtatt caacacccaa cgagggtttg cagagaaaac
     1801 gccttcgtta ctctactggc ctttgccatt cttgctttgg tccaccgtga ggttcggttt
     1861 gcgcggagtg accacatcac tcgttttcat aatgttcgta gcaatcgatg gcgcaacaag
     1921 aggtgctggt ccatttgtaa cgagttctgc gcttgacaat gcaatgtcga ttcagatgtt
     1981 cctgatagtt gtatctattc ccttgatggt cctggcttcc atcatcgaag aacgacgtaa
     2041 agtcgaagcg gctgccagag agaatgaaga gcgactgatg ctcgctctca atgccgcgca
     2101 aatggagacc tgggattggc ggataactga taatcgtttg atctggcccg acgaaacaag
     2161 aaatggattt ggcgtgaagt caaaggaaga cattagtccg gaaacatttt tccggctggt
     2221 ccatccagag gatcgtgaaa aggttgagcg cgcaacttca agtgctgttc gcgaaggcgt
     2281 gccttacgag attgagtacc gcatgttggc cgatggcgaa accaggtggt ttctgagcaa
     2341 aggaacagtt ttgcgtgatg aactgggcag gccggcacga atgcttggta tcggaatcga
     2401 tataaccgaa cgtaaaacag ctgaagaggc gttggaaaag atcaacgatc gcaaccaggc
     2461 aattctacga gccgttccgg atatgatgtt tttgttaaca agggatggag tttatctcga
     2521 ctattcagcg cgtgaggtcg ataaacttct tgtcccacca tcggacttta tcggcaaaag
     2581 catcaaagaa gtgatgcctc gcgatctcgc ggaaagcttc atcaagattt tgtcagaact
     2641 tagtgccact gatgaacctt atgtcgttga atatacgttg ccaatgaatg gtgagacgaa
     2701 atttttcgaa gcgcggttga ttctcgctga gggcgaaaat gtcttgacga ttgtacgcga
     2761 cgtcactgaa gctcatcagg cgatcgatag cgcgcgggaa agtcaggaga aggtcttaca
     2821 aagtaacaaa cagattcgcg cgcttgcggc gcgtctgata tctgcccagg aatccgagag
     2881 acgtcggatc tctcttttgt tgcatgacga cgtcagtcaa aatgttgcgg ctctcggatt
     2941 atcaatcagt cgactcaagc gcaagctgcc gacttcaaac gacgagctac tggatgaatt
     3001 gggccaactt ggatcgcaag catacgactt gacgacgcag atcaggcgcc tgtcgcacca
     3061 gttgcacccg gaagttctcg agcatctcgg actcgtaaaa gcgcttgaat ctcatgtgac
     3121 cgagttcggc tatgaagagc acattgagac gagcttcaaa gccgacgtta gaagcgaacc
     3181 attgccaatc gatctttcag tctgtcttta tcgagtcgca ttggagggct tgagaaatat
     3241 ctcgcgacac tccggcgcca aatccgccga agtaattctg agggagacca acggctgctt
     3301 gatgctacaa gtgtccgatt caggtttcgg cttcgacgtt gagcgtgcac gtcgcggaaa
     3361 tggaatcgga ttggccagtt ccgaagagcg catcagactc ctacagggta gcttcgaaat
     3421 acactccagt ccggaaaccg gtacaattat gactgctaga gttcccttaa tgaggtaata
     3481 atggaccgaa cccgaatctt gatagcggat gatcacaaga tgtttgttca aggtcttcag
     3541 ggactgcttg aagatgaatt cgatctcgtt ggtacagttg gtgatggaca cgcactggtg
     3601 gaagaagcgg ttcgtctcga tccggacgtc atcctggttg acatttcaat gccgagtttg
     3661 aacggactgg atgctgtgcg tcgtctgaag acgaagggac tcaacgcgtg catcatcttc
     3721 ctgacgatgc atgcagatga tcgattgctg gccgaagcat tcaggtgtgg tggaaagggt
     3781 tatgtcctca agcaatccgc aggtgaagag ctgatattgg cgatcaagca agttctggcg
     3841 ggtcacaaat atgtcacccc gttaattgcc actgagtggg tccaggattt aactgagcag
     3901 attggtgaga aacagaaact gacattaact ccgcgtcaga gagaagtgct gcaactcgta
     3961 attgaaggat gcactatgaa agaggtcgcg acgcggctag gcatctcgac tcggacggcc
     4021 gagtcgcata agtacgagat gatggaaggt ctcggagttg agagtaccgc ggaactgatc
     4081 cagtacgcga tcaagttggg tctgaccagg caatgaggat gtctcattaa tcggaaagaa
     4141 tcatttgata attatgattt gatatttgag aatttctatt tgttgcggca caaatccaag
     4201 aaca
//
LOCUS       DASSFP010000327         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1865070, whole genome shotgun sequence.
ACCESSION   DASSFP010000327 DASSFP010000000
VERSION     DASSFP010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865070"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1339
                     /gene="pdhA"
                     /locus_tag="VFH91_07255"
     CDS             215..1339
                     /gene="pdhA"
                     /locus_tag="VFH91_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET6862827.1"
                     /translation="MSKQTQSPPAVGAEDRASTSVIDPPDQNTRQQLSREQDAAKQKQ
                     KERLSQLDHESLIDLYRQMLLIRRFEEKAAEAYVAGKIGGFCHLYIGQEAVGVGAISA
                     IRQDDYVLGSYREHGQALAKGMSAKAIMAELFGKVTGCSKGKGGSMHMFDKDLGFLGG
                     HAIVGGQIPLATGTAFASKYQGKDQVTLCFFGEAAVNQGAFHESLNMAQLWKIPCIYI
                     CENNQYGMGTSLERAMSLRDIAQKASGYQMASEFVDGMDVLAVREATLRAVERGRKES
                     LPTLLEVRTYRFMGHSMSDPGNYRTRAEIEKYQERDPIKLFSASLLEEGIADQQKLDQ
                     LDQEIRAEVEEAVKFADESPEPPAENLYTDVYANPINFQK"
     gene            1353..2336
                     /locus_tag="VFH91_07260"
     CDS             1353..2336
                     /locus_tag="VFH91_07260"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HET6862828.1"
                     /translation="MAILTIRDALNQAIREELARDENVFLMGEEVAAYQGAYKVTKGL
                     LQEFGDKRVIDTPITELGFAGLGVGAAMAGLRPIVEFMTFNFSILATDQIINSAAKML
                     YMSGGQFKIPIVFRGPGGSAFQVSSQHSQAMESWYAYFPGIKVVMPSTPADAKGLLKS
                     AIRDDDPVVFIEQERMYGNKGEVPDDPDFTIPLGVADVKREGKDVTIVARSLMVPVAL
                     KAAETLEQQGVSCEVIDPRTIRPLDINTIVESVKKTNRVVVAEESHPTCGVNAEICTQ
                     IMEHAFDYLDAPVKRVSGADVPMPYAKNLENMAIPDVNRIVAAVREVAYLD"
     gene            2400..3683
                     /locus_tag="VFH91_07265"
     CDS             2400..3683
                     /locus_tag="VFH91_07265"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HET6862829.1"
                     /translation="MASKVIMPKLSPTMEEGQIARWLKKEGDKVSMGEPLAEIDTDKA
                     TMEMQALADGVLRKIIIKEGEAAPLGQLIAIIGGQDEDISSLLNESPAPAAAQPAKPQ
                     DEAPAAEVKTPQLKAVPQGNGTQAQEAAVGGRLIVSPLAARMAAEAGIDLRSLQGSGP
                     GGRIIKKDIESVISQPRTAAPTQLRAVESKPFQPAIVAGASAYRDEPATEIRKTIAKR
                     LVTSLGPVPHFFLTSEIEMDRAAEMRKGINALDPDLKISINDIIIKVTAAALIQHPEV
                     NASFQEKVIRYYEQADIGVAVAIEDGLITPVIRGANLKSLSQIAGEVRELAERARNRR
                     LKPEEYTGATFSISNLGMFGIDEFTAVINPPEGAILAIGSMTPKPVVRDNEIVIRQIM
                     RVTMSCDHRVIDGATGAKFLQTFKKILENPLYLVV"
BASE COUNT         1009 a          925 c          994 g          839 t
ORIGIN      
        1 aaaaccatcg cgacagagac cgaagagtct cagaagcagc acagagaagg ttcagattag
       61 gacaccactg tttattgcgc gcttttgcac gcctttgtaa atctcgcgta taattccccg
      121 cttaagtttt gagtccttag aaacacgatc taattcttaa acgcccgcta agatgcgggc
      181 attgtttagt gcgcagttct gcattctgct tatcatgagt aaacaaaccc aatcgccccc
      241 ggcagtcgga gctgaagata gagcctccac atcagttatc gacccacctg accagaacac
      301 acgtcaacaa ctgagtcgcg agcaagacgc cgcgaaacag aaacaaaaag agcgactaag
      361 ccagcttgat cacgaatcgt tgatcgatct ctatcgccag atgttgctta tcaggcgctt
      421 cgaagagaaa gctgccgaag catacgtggc tggaaagatt ggtggctttt gtcacctcta
      481 catcggtcag gaagccgttg gcgttggagc aatctcagcc attcgccagg acgactatgt
      541 gcttggaagt tatcgcgaac atggtcaggc gctggctaaa ggaatgtcgg caaaagcaat
      601 catggctgaa ctgtttggca aggtcaccgg ttgctcaaag ggcaagggtg ggtccatgca
      661 tatgttcgat aaggacctcg ggttcctggg aggacatgca atcgttggtg gacaaatccc
      721 gttagcgacc ggcactgcat tcgcgtcgaa gtatcagggc aaggatcagg tcacgctctg
      781 cttcttcggt gaggcagcag taaaccaggg agcatttcac gagtcgctaa acatggccca
      841 actctggaag attccctgta tttacatttg tgaaaacaat cagtatggca tgggaacttc
      901 gctggaacgc gctatgtctt tacgtgacat tgctcagaag gcgagcggtt atcaaatggc
      961 ctccgagttt gtagacggca tggacgtgct ggcagttcgt gaagcaacgt tgcgcgcagt
     1021 cgaacgcggg cgaaaggaat cattaccaac cctgttggaa gtgaggactt atcgctttat
     1081 gggccactca atgtcagacc ctggtaatta tcggaccaga gcagaaatcg agaagtacca
     1141 ggagcgcgat cctatcaaat tgttctcagc aagcttgctc gaagaaggta ttgcagatca
     1201 gcaaaaactc gatcaactcg accaggaaat ccgagctgaa gttgaagagg cagtgaagtt
     1261 cgccgatgag agtcccgaac cgcctgctga aaatctctac accgacgtct acgcgaatcc
     1321 aataaatttt caaaaataga tagatactaa atatggcgat tctcacgata cgtgatgctc
     1381 ttaatcaggc gatacgggaa gagttggccc gtgatgaaaa cgttttcttg atgggcgaag
     1441 aagtcgcggc ctatcaggga gcttataaag tcaccaaggg tctgcttcaa gagttcggcg
     1501 acaagcgagt tattgacacg ccaatcactg aactcggctt tgccggactt ggagttggtg
     1561 cggcaatggc aggacttcga ccaattgtcg aattcatgac gttcaatttc tcgattctcg
     1621 ctactgacca gatcattaat tcggctgcca agatgcttta tatgtccggc ggccagttta
     1681 agattccgat tgtcttccgt ggcccaggag gttcagcatt ccaggtttca tcccagcact
     1741 ctcaggcgat ggaatcatgg tacgcatact ttccaggcat caaggtagtg atgccttcga
     1801 cacctgccga cgccaaggga ctactcaaat cggccattcg agatgatgat cctgtagttt
     1861 tcatcgaaca ggaaagaatg tacggcaata agggtgaagt tcccgacgac ccagacttca
     1921 cgattcccct cggggtcgct gacgtaaaac gtgaaggcaa ggacgtaact atcgtcgctc
     1981 gatctctaat ggtgcctgtc gcgctgaagg cggccgagac acttgaacag caaggcgtct
     2041 cgtgtgaagt aattgaccca agaacaatca ggcctctgga catcaatacc atagttgagt
     2101 ccgttaagaa gacgaaccgc gtagttgtgg ccgaagagtc gcatccaact tgtggcgtga
     2161 acgctgagat ttgcactcaa attatggaac acgctttcga ctacctcgac gcacctgtga
     2221 agagagtttc cggcgctgac gtgccgatgc catacgcgaa gaatcttgag aatatggcga
     2281 tccccgacgt taataggatc gtcgcagccg ttcgcgaagt agcatatctc gattaatacc
     2341 tatgactccg atgggcgccc acggagggag acccatacat ccaaaataag gaatcgcgca
     2401 tggccagtaa ggtaattatg ccgaaacttt ctcccacgat ggaggaaggt cagatagcca
     2461 gatggttgaa gaaagaaggc gacaaggtct cgatgggtga gcctctcgcc gaaattgaca
     2521 ccgacaaggc tacgatggaa atgcaggccc tggctgatgg cgtgcttcgc aaaattatta
     2581 tcaaagaggg agaagcggct cctctgggcc agctgatagc gatcatcggt gggcaggatg
     2641 aagatatcag ttcccttttg aacgaatcgc cagctccagc ggcggcacaa cctgccaagc
     2701 cacaggacga ggctccggcc gcagaagtaa aaactcctca attaaaggct gtgccgcaag
     2761 gaaatggcac tcaggctcaa gaagcggctg ttggcggaag attaattgtc tcacctttag
     2821 cagctcgaat ggccgcagag gcaggcatcg acctacgctc gttgcaaggt tctggaccag
     2881 gtggtcgcat cattaagaag gatatcgagt ctgtaatcag ccaacctcga acagcagctc
     2941 caactcaact tcgcgcggta gagtcgaagc cattccaacc ggcaattgtg gcgggcgcgt
     3001 cagcatatcg agatgagcct gctacggaga ttcgtaagac gattgcaaaa cgactggtca
     3061 cttcgctcgg tccagtccca cactttttcc tgacctccga aatcgagatg gaccgcgcgg
     3121 ctgagatgcg caagggcatc aacgcgctcg atcccgatct gaagatcagc atcaacgaca
     3181 tcatcataaa agtgactgcg gcagcgttaa tacagcaccc tgaggtgaat gcttcgtttc
     3241 aggaaaaggt aatcagatat tacgagcagg ctgatattgg agttgcggtg gcaattgaag
     3301 atggtctcat cacgccggtt atcagaggcg ccaatctgaa gtcgctcagt cagatagctg
     3361 gtgaggtgcg agaacttgct gaacgtgctc gaaaccgcag gttgaaaccc gaggaatata
     3421 ccggcgcgac gttttcgatc agtaatctcg gaatgtttgg tattgatgaa tttacggcag
     3481 taataaaccc accggaaggc gcgatcctgg cgattggttc aatgactccg aagccagtgg
     3541 tgcgtgacaa cgagatagta atccgtcaaa tcatgcgcgt gacgatgtcg tgcgatcatc
     3601 gcgttattga cggagctacc ggggcaaagt tcctacagac tttcaagaag attcttgaga
     3661 atccactcta cctggtcgtc tgagcctgga cacaaatctt tccagatcat cctcagacta
     3721 cgccgattaa acagattctt tacttagcac gcctttgcgc cgtgcct
//
LOCUS       DASSFP010000328         5918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2031207, whole genome shotgun sequence.
ACCESSION   DASSFP010000328 DASSFP010000000
VERSION     DASSFP010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031207"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFH91_07270"
     CDS             <1..353
                     /locus_tag="VFH91_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET6862830.1"
                     /translation="SSVDELNSAMVCTGFPYNVRERPNFARDFTNFTLQAQAVRRDGS
                     AAIDLAYVACGRFDGFWEDGLNPWDIAAGVLLIQEAGGLVTNFQNEPLNIYTSKVLAT
                     NGLIHHDMLRVLHM"
     gene            518..970
                     /locus_tag="VFH91_07275"
     CDS             518..970
                     /locus_tag="VFH91_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862831.1"
                     /translation="MNIKYRKLVDDTRSEGFSFYPLLQVFLRNGLNMRPVLALVDSGS
                     VDCVFPASIGEVLGVDIPSGQPYEFHGFDLRLVPGFVHKLHLQVAGFTHWVEVDAVFL
                     ESEGMAILGQRGFFESYQIVFERWARQFEVKTKTDAMIRNRRGYGRRR"
     gene            complement(967..1176)
                     /locus_tag="VFH91_07280"
     CDS             complement(967..1176)
                     /locus_tag="VFH91_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862832.1"
                     /translation="MPTTTKEEAFAELVREYENMWVAIIEKDSVEFVVGHGSTAAQAA
                     QEATQKGHPQATLFRVPSFKNRFVY"
     gene            complement(1358..2032)
                     /locus_tag="VFH91_07285"
     CDS             complement(1358..2032)
                     /locus_tag="VFH91_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6862833.1"
                     /translation="MSNNGTFQAKENARPANGRAMLPQWALEIIRPIVGAVSKVAWKI
                     EFSGVENIPQDGGVIVAANHQTYIDPFWISLPIKRPTRYLAWSAAFRWPIVGQCLIWL
                     GAWPLALEGSDPAAIRRSLQWLREGGAVVIFPEGGRSTVTGGLERFKAGAIRLALEAN
                     VPILPVTIRGGNKVWPRGWRFPRPGKVVITYHPLYLPEKAPGEETRAYARRESEHLAG
                     VIASAL"
     gene            complement(2029..2277)
                     /locus_tag="VFH91_07290"
     CDS             complement(2029..2277)
                     /locus_tag="VFH91_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862834.1"
                     /translation="MKGVAIDKTVLLYELERWCSFADCNARVKIGITKSEAFDYEGFE
                     CDVCNRWTPDTLTEKDIPEWWPELKLDHGRGLSDSDVN"
     gene            complement(2287..3030)
                     /locus_tag="VFH91_07295"
     CDS             complement(2287..3030)
                     /locus_tag="VFH91_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862835.1"
                     /translation="MPPKTPMGEQRNWKRIRERLGLNLPIQVHCRETNEFEWKEASRL
                     INVTPFGAGFTLKHPTEKGRLLHMVIPMPRQLRVFDHLELQYRIWAIVRHAKLISTSK
                     SDEILFQVGVAFIGKRPPATYEAQPWRRFEVVIGPRKLTNVIEMVRPLKPVDQRTITR
                     HNIAVDLKVETLDDSGATVTSELTVTEDISRYGARIFTTLETQVGRFIRLSSTHYNIT
                     TFAAVRAVSKGEDGIRRIHVEFIGREWPL"
     gene            3355..>5918
                     /gene="gyrA"
                     /locus_tag="VFH91_07300"
     CDS             3355..>5918
                     /gene="gyrA"
                     /locus_tag="VFH91_07300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET6862836.1"
                     /translation="METTSERNHINIEDEMRRSYLDYAMSVIIGRALPDVRDGLKPVH
                     RRVLWAMHELGNTYNKTYKKSARIVGDVIGKYHPHGDMPVYDTIVRLAQDFSMRYPLI
                     DGQGNFGSVDGDAAAAMRYTEIRMARLANEVLADIEKETVDFQPNYDESLPEPKVLPA
                     RVPNLLINGSDGIAVGMATKIPPHNLTEVVNATLALLKDPELKVEDLAKIVTGPDFPT
                     GGFIYGRNEIKKSYLEGRGVLQLRARAGIDRIGRGSQERDAIVITEIPFQVNKARLIE
                     RIAELANEKKLEGISDLRDESDRTGMRIVVELKRDAVPQVVLNKLYKLTPMQSSFGVI
                     NLAIVNGQPRVLNLKQMLECFIEFRREVVKRRTEYDLRKARARAHILEGLTKAIDALD
                     AIVTLIRNSRSVDEARQWLTAQWTTINEVKDWKGIPSDVALKSHLGKIQKTMDRLAFS
                     DIQAQAILDLQLRRLSALERQKIIDEYESIIKLIAELEEILSNERSLRRVISTELEEV
                     KKDFGDERRTEIVDEGVELSIEDLIADEDVAITVTNSGYIKRTPITTYQRQGRGGKGR
                     FGAAAKNGDFVEHLFIASTHAYLMIFTDDGMVYKIKVHEVPDAAASARGKAVVNLINI
                     PSDRRLAGVVPVRDFEEGRHVVMVTRKGVIKKTSLADFQNIRSNGIIAINVDEGDRLL
                     DVVLTDGTKRIFIATHNGLAIRFDEKNVRPMGRATRGMRGIDLRPDDFVVSVCPVSAE
                     DTERMLSVSEQGYGKQTPITTYRLQSRGGKGVINMKTTEKTGKVVAVFPVEDDAEIMI
                     ITQQGKLIRLEAADIRKTGRSAQGVRLIKTDASDKVTSASLVEAAAEDETEETPAG"
BASE COUNT         1459 a         1462 c         1521 g         1476 t
ORIGIN      
        1 tctcttccgt cgatgaactc aacagcgcaa tggtgtgtac gggcttccct tataacgttc
       61 gcgaacgacc taactttgcc cgtgacttca ctaatttcac gctgcaggcg caggccgtga
      121 gaagagacgg ctcggcggca attgatcttg cttatgttgc ttgcggccgg ttcgatggtt
      181 tctgggagga tgggttgaat ccctgggata tcgctgccgg agtattgttg atccaggaag
      241 caggcgggct tgtcaccaac tttcaaaatg agccattgaa tatttacacc tcaaaagttc
      301 tcgccaccaa tggtctgatt catcacgaca tgctccgcgt actgcatatg tagacagaag
      361 ttatagatac gcgctacgct ccttaatcca ttccttacac aaaaaccatt cagtgagaaa
      421 cgggcaagat taaacccaaa gggttcgtgt aagccatagg gttcgattcc cacagcctcc
      481 gtgctaaggc gctatcacag agaggacagt tcaggaaatg aatatcaagt atcgcaagct
      541 agtcgatgac acaagatcgg agggtttctc gttttatcca ctgcttcaag tatttcttcg
      601 caatggcctt aacatgcggc cagtgcttgc actagtcgat tcaggttcag tcgattgtgt
      661 atttcctgct tcgataggcg aagtcttagg ggtagacatt ccttcaggac agccctatga
      721 attccatggt ttcgatcttc gactagttcc gggctttgtt cataagttgc atctccaagt
      781 tgccggtttc acgcactggg ttgaggtcga tgcggtattc ctagaatccg aaggaatggc
      841 gatattgggc cagcgaggtt tcttcgagag ttatcagatt gtctttgagc gatgggcgcg
      901 tcaatttgaa gttaaaacca aaacagacgc gatgattcgt aacagacgtg gatatggtcg
      961 gaggcgctaa tagacaaatc tattcttgaa ggaaggaact ctgaatagtg tcgcttgagg
     1021 gtgccccttt tgtgttgcct cttgagcggc ctgagctgcg gttgatccat ggccgacgac
     1081 aaattccaca ctatctttct cgattatagc cacccacatg ttttcgtatt ctcgaacgag
     1141 ttcggcgaaa gcttcttctt tagttgttgt aggcatcaca cgaatccccg atttctcttg
     1201 attccctaga tgcattttgc cctgcttgga tgggcgacgc aagctttccg gcttgcatca
     1261 acctggctaa cgttgttctt acttgattgg gacaaaggcc atgccagaac tgcggtagat
     1321 ttgagatttg gacactaccc ccaaccaagc aggtcatcta cagcgcggaa gcaatcacgc
     1381 cggcaaggtg ctcgctttcg cggcgagcat acgcacgtgt ttcttctcca ggtgctttct
     1441 ctggaagata tagcgggtga tacgtgatca cgactttgcc ggggcgggga aatcgccagc
     1501 cacgtggcca aactttgtta ccgcctctaa tcgtcacagg cagaatgggc acgttagcct
     1561 ctagcgctag tcggattgct cctgctttaa atctctccaa acctccggtc actgtacttc
     1621 ttccgccctc tgggaaaatc actacagcgc ccccttctcg caaccattgc agtgaacgcc
     1681 ttattgcagc cggatcactt ccttcgaggg caagaggcca cgctcccagc catattaggc
     1741 actgtccaac gatgggccaa cgaaaagctg cactccatgc gagatatctg gtgggccgtt
     1801 taatgggcag tgatatccaa aatggatcga tgtacgtctg atgattggcg gcaacaatta
     1861 ctccgccgtc ctggggaatg ttttcgacgc ctgagaattc aatcttccac gcgaccttac
     1921 ttacggcacc gacaattggc cggattatct cgagtgccca ttgaggcaac atagcgcgac
     1981 catttgcagg tcgcgcattc tctttggcct ggaatgtgcc attgttactc aattcacgtc
     2041 agagtccgac aatccgcgcc cgtggtccag cttcagttcg ggccaccatt caggaatatc
     2101 tttttctgtt aaggtatccg gtgtccagcg attgcagaca tcgcactcaa agccttcgta
     2161 atcgaaagct tcagattttg tgattccaat ttttacgcgc gcattgcagt ctgcaaaact
     2221 gcaccatcgt tccaactcat acaacagaac cgttttgtcg atcgccacgc ccttcataga
     2281 gacattctac aacggccatt ccctgccaat gaactcgaca tgaattctcc tgataccgtc
     2341 ttcgcctttg ctgacagccc gcacagccgc aaaggtggtg atgttgtagt gagtgctact
     2401 taagcgaatg aagcgtccga cttgagtttc gagcgtggta aaaattcgtg ctccgtacct
     2461 gctaatatct tccgttactg tcaattcgct cgtaactgtt gctccactgt catctaaagt
     2521 ctctaccttg aggtcaacag caatattgtg tctggtaatc gtgcgttgat cgactggctt
     2581 taaaggacgc accatctcga tgacattcgt aagcttccgt ggtcctatta ctacttcaaa
     2641 gcgtctccat ggttgagcct catacgtagc cggcggtcgt ttgccgatga atgcaactcc
     2701 aacttgaaac aggatctcgt cgctttttga cgtgctgatg agtttagcat gtctcacgat
     2761 ggcccaaatt ctgtattgca gttcaaggtg atcaaagact ctcaattgcc ttggcattgg
     2821 tataaccata tgaagcaacc ttcccttttc cgtcggatgc ttgagcgtga atcccgcacc
     2881 aaaaggcgtg acattgataa gtctcgaagc ttccttccac tcgaactcgt tggtctcgcg
     2941 acaatgcacc tgaattggca gatttagacc taaacgttcg cggatccgtt tccagtttcg
     3001 ctgttcaccc atgggagttt tgggaggcac gcggggcgat ttttcgccct cgctcgtttc
     3061 ggaactcagg gcgttacaaa aaactatttc gaatgctcac gaagctattg atttcattgg
     3121 ggttttcgtc gaagtaggtg tttgccaaaa cgactcaaaa aaggctaaaa tgcacctgaa
     3181 tttgatgtcg aaacacccat ccgcttctca gctgaacgaa cagaacatcg agcgagtttt
     3241 agtcacttcg taaggtttag atctgtgcgc attgaatgac aaagaactta gcgaaacggc
     3301 agttagagaa gtcagggccc tgggattcgc ggattgtact tgaaaggcta ttagatggaa
     3361 acaacttctg agcgcaacca tataaatatc gaagacgaga tgaggcgttc ctatctcgat
     3421 tatgcgatgt ccgtaattat cggccgcgca ttgcctgatg tgcgagatgg gctcaaaccg
     3481 gtgcaccgcc gagtcttatg ggccatgcac gagctgggca acacttataa caagacgtac
     3541 aagaaaagtg cacgcatcgt tggagacgtt attggtaagt atcacccgca cggtgatatg
     3601 cctgtttacg acacgatagt tcgtctcgcg caggatttct ccatgcgcta tccgttgatc
     3661 gatggccagg gcaacttcgg ttcggtcgat ggggacgcgg ctgcggcgat gcgctacacc
     3721 gaaatccgaa tggcgcgtct tgctaacgaa gtgttggcgg acatcgagaa ggagacggtc
     3781 gattttcagc ctaactacga cgaatcgcta ccagagccaa aagtcctgcc cgctcgtgtt
     3841 cctaacctgc tgataaacgg atcggacggc atcgcggtag ggatggcgac gaagattcca
     3901 cctcataacc tgactgaagt ggtgaatgcg acgctggcgt tgctcaaaga cccggagctg
     3961 aaagttgaag atctggccaa gatcgttact ggcccggact ttccgaccgg cggcttcatc
     4021 tacggacgaa acgaaatcaa gaaatcgtac ctcgagggtc gtggagtact tcaattacga
     4081 gcccgcgcag gtatcgatcg aatcggccgc ggctctcaag agcgagacgc aattgtaatt
     4141 actgaaattc cgttccaggt aaacaaagcc cgattgattg agcgaatcgc cgagctggca
     4201 aatgaaaaga agctcgaggg catttcggat ttgcgtgatg agtcggatcg aactggaatg
     4261 cgtatcgtcg tagagctcaa gcgcgatgct gttcctcagg tcgttctcaa caagctctat
     4321 aagcttacgc cgatgcaatc gtcatttggg gtgatcaacc tcgcgatcgt aaatggccaa
     4381 ccgcgcgtcc tgaatctcaa acagatgctc gagtgtttta tcgagttccg acgcgaggtt
     4441 gttaagcgcc gcactgaata cgatctgcga aaagccaggg cgagagctca tatactcgaa
     4501 ggtcttacca aagccatcga tgctctcgac gcgatcgtta cattgattcg caactctcgc
     4561 tccgtcgacg aagcccgcca gtggttaaca gctcaatgga ccaccatcaa tgaggtcaag
     4621 gattggaagg gtattccgtc ggatgtggcg ctcaaatcgc atctcggcaa gattcagaag
     4681 acgatggatc gcctggcgtt ttctgacatt caagcacagg ccattctcga cttgcagttg
     4741 agacgtctgt cagcactcga acgtcagaag attatcgacg aatacgaaag catcatcaag
     4801 cttatcgcag aactcgaaga gattctttca aacgagcgtt cgttgagaag agtaatctcc
     4861 actgagctcg aggaagtgaa gaaagatttt ggtgatgagc gccgtacgga aatcgtcgat
     4921 gagggagttg agctctcgat tgaggactta attgccgatg aggatgtggc gataaccgtt
     4981 acaaacagcg gttacatcaa gcgcacacca atcactactt accagcgaca gggaagaggg
     5041 ggcaagggcc gttttggagc cgccgcaaag aatggtgact ttgtcgagca tctattcata
     5101 gcttcaaccc atgcgtatct gatgatcttt actgacgatg gcatggtcta caagatcaag
     5161 gttcacgagg ttcccgacgc tgcagcttcc gcacgcggaa aagcagttgt gaacctgatt
     5221 aatattccat ccgatcgccg tcttgccggt gtcgttcccg tccgtgactt tgaggaaggt
     5281 cgtcatgtgg taatggtcac gcgaaaaggt gtgatcaaga agacttcgct ggctgatttt
     5341 caaaacattc gatctaacgg cattattgcg atcaatgtgg atgaaggtga tcggctgctc
     5401 gacgtagttc taaccgacgg aacgaaacgg atcttcatag ccacgcacaa tgggttggca
     5461 attcgcttcg acgagaagaa cgtcaggccg atgggtcggg ccacacgcgg aatgcgcgga
     5521 atcgatctca ggccggacga ttttgttgtt tcagtttgcc ctgtctcggc agaagatact
     5581 gaacggatgc tgtcagtttc tgaacagggt tatggaaagc agacgccgat tacaacatac
     5641 cggctacagt cacgcggtgg aaaaggcgtc atcaacatga aaacgactga gaagaccgga
     5701 aaggtagttg cggtgtttcc tgttgaagac gacgcggaaa tcatgattat cacgcagcaa
     5761 ggcaagttga tcaggctgga agccgcagac attcgcaaga ctggccgtag cgcccaggga
     5821 gtcaggttaa taaagactga cgcctcggat aaggttacaa gcgcgtcgct ggttgaggct
     5881 gctgcagaag atgagacgga ggaaacgccg gcgggtta
//
LOCUS       DASSFP010000329         6948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1123617, whole genome shotgun sequence.
ACCESSION   DASSFP010000329 DASSFP010000000
VERSION     DASSFP010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123617"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFH91_07305"
     CDS             complement(<1..466)
                     /locus_tag="VFH91_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6862837.1"
                     /translation="MPSTLKLQTTPSVKELTSAPEVLEALAYFDANLEHITAEHIKIS
                     SVPASPFDERQRAEYLREKFVKLGLEEVTIDPEGNCLGLFSGNSSSPLLVVSAHLDTV
                     FSKDTDFTVHRRGNRLFGPGISDDASGLVALIAIIEALKTSQIKTRGSILFVA"
     gene            complement(518..1732)
                     /locus_tag="VFH91_07310"
     CDS             complement(518..1732)
                     /locus_tag="VFH91_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HET6862838.1"
                     /translation="MALEELVYDWNVVEPSFAAPNRHIGFDDETLRDGLQSPSVCEPP
                     VEQKIKLLHLMNELSIDTADIGLPGAGGTHAASVERLAREIADQKLKIRANCAARTHR
                     NDIVPIVEISQRAGIPIEACTFIGSSSVRFFAEDWTLEKLLHLTEEAVSFAVSEGLPV
                     MYVTEDTTRAKPDVIRALYSTAIRSGAKAVCVCDTVGHSTPDGARAVVSFVKAIIEEH
                     GGNIRLDWHGHQDRGLGVINSIAAIQGGADQVHGSALGIGERVGNTPMDQLLVNLKLM
                     GWIDNDLSRLGTYCKTAAEACQWSVPFNYPVFGRDAFRTATGVHAAAVIKSYKKGDQR
                     LADIVYSGVPAGEFGLEQVIEIGPMSGKSNVIYWLEKRGLAADESRVNRIYEYAKAAS
                     AVLEESEIRKLL"
     gene            2008..2580
                     /locus_tag="VFH91_07315"
     CDS             2008..2580
                     /locus_tag="VFH91_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HET6862839.1"
                     /translation="MPRKTIALGSDRAAKIMAVRASVARIATIDPSWADANVVARRVE
                     VNVPAMPLTDWQLMQGARERALAAEQALKVRRLDADIYVGLEGGFHSISIEGEWHTFL
                     RGWAYATDGERGAFGAAPSISVPPEIVKSVVEGRRELGEVIDQVTGGRDIRSRQGAWG
                     VLSRDLVTRSMSFELALIAALAPFYNAQMY"
     gene            2600..3220
                     /locus_tag="VFH91_07320"
     CDS             2600..3220
                     /locus_tag="VFH91_07320"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET6862840.1"
                     /translation="MDELVAQVNHDEPPPHQRSNAGWVEVIAGSMFSGKSEELIRRLR
                     RAKIARQKVQVFKPEIDSRFSDNQIVSHSEMRHESANSRSATEVLAKVEPDTEVVGID
                     EGQFFDNDLVDVANELARRGVRVIIAGLDQDYMGRPWEPMPQLLAIAEYITKTHAICM
                     KCGQPANYSQRTFQSEERVAVGASDKYEARCRNCFVPHADAPTLNE"
     gene            3552..4118
                     /locus_tag="VFH91_07325"
     CDS             3552..4118
                     /locus_tag="VFH91_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6862841.1"
                     /translation="MSDPSDITELLIRWSTGDKDALQKLMPLVERELHRLAHYYMRHE
                     HVGHTLQTTALINEAFLRLVDQTRVQWQNRAHFFGIAAQIMRRVLLNYARDRHRQKRG
                     GHAVQVSLSDVAIISDDKSAELIALDEALERLAAIDLRKSRVVELRFYGGLSVEETAE
                     VLGVSTITVNRDWKMAKAWLSREIRNVL"
     gene            4108..6666
                     /locus_tag="VFH91_07330"
     CDS             4108..6666
                     /locus_tag="VFH91_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6862842.1"
                     /translation="MSSDQWRRIEDLFHAASQLHPSDQLDYLKRESGNDPAIVAEVQS
                     LIEASNDALFETPAVSLGLKVLSNASKPGALAGRTIGHFKIIKLLGAGGMGEVYLATD
                     LNLQRQVALKFLNHGFGNDEWLRAQLILEARAVAQLENHNICSIHGLENEDGQDFIEM
                     QYVEGETLQTRLSSGPLSIDLALDFAEQVAGALSAAHEHGIVHRDVKTQNIIVTPDDQ
                     IKVLDFGLAKLVKPIEPTQSKSEQHQTGASGILGTVAYMSPEQAEGDEIDARSDIFSF
                     GIVFYEMLTGENPFRRDDKFQTLEAIKNEHPDLSLDLPKQLLNILGGCLEKDRDNRFK
                     SSKVLRSEIQALRRSREPSPFTTWMQSRNFKYLAAAAAVLLVFILASSAFVYHKTSSV
                     HSIALLQIQNRSGDANLDYLSQGLTRSLFDKFSYLTRLQVKAPSTMPFDREQNVDLKR
                     AGRDLSVESVLSGEILKHDQSLLLRLSLSDTENGGTKWQEEFNLDAANMFVLQDDITR
                     KVVSSLGLWLIGGERKRLNKHQTDNDEALRKYMLGRQFSGLKRSRENMQKAIGYFEQA
                     IELDPSFAEAYSGLSDCYAVMNNVANGPIAPSVAMAKARWSAQQALDLDDSLPEAHTS
                     LGIINLRFDWNWEKAESEFKRAIELKPVYAPAHYWYSNLLSALGRHDEAIRESKTSRD
                     LDPYSLLAEMNYGRSLYYARRYAEAEDYFADLLSQNDQYPSFQHMMALVQLQLGRYQP
                     AIELLEQMRKNDPLYGTAALGYAYGKNGQRNEAQNILRALDEASTREPVPPHEKAIVY
                     LGLGDKDQAFKYLDQSLEERFASMAFLNADPLYDDIRSDVRFRELEQKVGLSLP"
BASE COUNT         1783 a         1762 c         1768 g         1635 t
ORIGIN      
        1 tcgccacaaa caagatagaa ccacgtgttt ttatttgaga ggtctttaag gcttctataa
       61 tcgctatcaa tgcgacaaga ccggacgcgt cgtcagagat gcctggtcca aacaatcgat
      121 tgccccggcg atgcacagtg aagtcagtgt cctttgagaa gactgtatcg agatgagcgc
      181 tcacgacgag cagcggcgac gacgagttgc cggaaaaaag ccccagacaa ttgccttccg
      241 gatcgatcgt cacttcttcc aggcccagct taacgaactt ctcccgtaaa tactcggccc
      301 gttgtctttc gtcgaacgga cttgccggca cactgctgat tttgatgtgc tctgcggtta
      361 tgtgttcaag gttagcgtcg aagtaggcga gagcttctaa aacctccggc gcactggtaa
      421 gttccttgac gcttggagtt gtctgtagtt ttaaggttga aggcatgaag actggtatta
      481 cccctggggc tgtggaccgt acacgctttg attagaccta taacaatttt ctgatttcgc
      541 tttcctcgag caccgcgctg gccgctttcg catactcgta gattcgattg acgcgacttt
      601 catccgcggc gagaccgcgt ttctcgagcc agtaaatcac atttgattta ccgctcattg
      661 gtccaatctc gatcacttgt tccaaaccaa actcacctgc gggcactcct gaatagacga
      721 tatcggccag ccgctgatcg cccttcttgt aactcttgat tacagcagca gcgtgaacgc
      781 cggtcgcagt tcgaaaagca tcgcgaccaa atacaggata gttaaaaggc acggaccatt
      841 gacaagcttc ggctgcggtt ttgcagtaag tgccaagtcg cgagaggtcg ttgtcgatcc
      901 agcccattag cttaagattg accaacaatt ggtccatcgg ggtgttgcca actctttccc
      961 cgattcccag cgccgaaccg tgtacctgat cggcgccacc ctggatcgcg gcgatggagt
     1021 tgatcacacc caatcctcta tcttgatgac cgtgccaatc gagtcgaata ttgccgccgt
     1081 gctcctcaat gatggcctta acaaaactga cgactgcgcg tgcgccatcg ggagttgagt
     1141 gcccaaccgt gtcgcatacg caaactgcct tcgcgccgct cctgattgca gttgagtaga
     1201 gcgcgcgaat gacatctggt ttagctcgag ttgtgtcttc ggtgacatac atcaccggta
     1261 aaccctcact caccgcaaag gaaacagctt cttccgtcaa atgaagcagt ttctcaagcg
     1321 tccaatcttc agcaaaaaat ctcacggatg atgagccgat aaaggtgcat gcttcgattg
     1381 gaataccagc gcgctgtgaa atctcgacga ttggaacaat gtcattgcga tgagtgcgag
     1441 ccgcacaatt agctcgaatc ttcaatttct gatcggcaat ctctcgagca agtcgctcaa
     1501 cactagctgc gtgagtaccg ccagcgccgg gtaaaccgat atcggcagta tcgatgctca
     1561 attcgttcat gagatgaagg agcttaatct tctgttcgac tggcggttcg caaaccgagg
     1621 gtgattgcaa tccatcgcgt aaagtctcgt cgtcgaagcc tatgtgccgg ttaggtgcgg
     1681 caaaactcgg ctcaacaaca ttccaatcgt agactaattc ttcgagcgcc attttgactt
     1741 gctccaacgt aaatccactg ccgtaaaatt aactgaaatt gattacaaca accgtccaac
     1801 ccaaagtcaa gccaacgatt ttgttggttt gtttggtaat gaggggagag aatggaacct
     1861 ataggactaa tagaacccgt cgattactga tcaggcggct tttgacgagt tgtacttcga
     1921 tgttatagcc tctcacccgc ttcagcctct ctagttcttg agactctgaa acgcttttca
     1981 aaggtccacg ttcgaacaaa agcgtctgtg cccaggaaaa caatcgcctt aggatctgat
     2041 cgcgccgcaa aaatcatggc tgtgcgtgct agtgtagctc gcattgccac gatcgatccg
     2101 agctgggcgg acgctaacgt agtggctcgc cgcgttgaag ttaacgttcc agccatgcct
     2161 ttaactgatt ggcagttgat gcaaggcgct cgagaacgag cgctcgcagc agagcaggcg
     2221 ctcaaggttc gacggctcga tgctgacata tacgttggtc tcgaaggcgg tttccactcg
     2281 atttcaattg aaggtgaatg gcacacattt cttcgaggtt gggcttacgc tacggacggc
     2341 gaaagaggag cctttggagc tgcgccttcg atttcagttc ctcctgagat tgttaaaagc
     2401 gtagttgaag gacgtcgaga gttgggagaa gttatcgacc aggttaccgg cggtcgcgac
     2461 atacgaagtc gacaaggcgc atggggagtt ttgtcgcgcg acctcgtgac tcgttcgatg
     2521 agttttgagc tggctttaat agccgcctta gcgccttttt ataacgccca gatgtattaa
     2581 gtaacaggac aaagcataga tggatgaatt agtggcacaa gtaaatcacg atgaaccacc
     2641 accgcatcag cgatcaaatg ccggatgggt ggaggtgata gccggctcga tgttttccgg
     2701 aaagtcggaa gagctcattc ggcgattgcg tcgcgcaaag atagcgcgac agaaggtcca
     2761 ggtcttcaaa ccagagatcg actcaagatt ctcagacaat caaatagtgt cgcattccga
     2821 gatgcgccat gaatcagcaa atagtcgatc cgcaactgag gtcttggcaa aggtcgaacc
     2881 ggataccgaa gttgtaggga tagatgaagg tcaatttttt gacaacgatc tggtagacgt
     2941 cgcaaatgaa ctcgcgcgtc gcggcgtgcg agtgattata gcgggactcg accaggacta
     3001 catgggtagg ccctgggagc ccatgcctca gctgctggcc atcgcggaat acatcaccaa
     3061 gacacatgcc atctgcatga agtgcgggca accagcgaac tactcacaga gaacttttca
     3121 atcggaagag cgcgtggcgg ttggggcatc tgataagtat gaagcgcgat gtcgcaattg
     3181 cttcgttcct catgcggacg cgccgacttt aaatgaataa atcttaaaac ccccgaccac
     3241 ctcatgactt aaagactacg acggcgacgg ttaacgaaga gcaggctttt ctgggcgctt
     3301 tacgttgcaa gaaaccgaac gcggctatat aactgctgca gcactaagca agctgcagca
     3361 ctaagcaaga ggacagccgg gagtgggctg ggcctgcgtg gggtgaatat ctcttgttat
     3421 ccagtcaccg tcacctcata acgcaccaag cgtaaccaaa gagcagcatt cccgcgcaat
     3481 tgctacggtt caaccggagc aactcggtta tctggccggt tctaatactg caatcgacgg
     3541 agattccccc gatgtccgat ccttccgaca ttacagagct cctaatccgc tggagtactg
     3601 gcgataaaga tgcactgcaa aaattaatgc ctctcgttga aagagaattg catcgtttgg
     3661 cgcattacta catgagacac gagcacgtcg gtcatacctt gcagacgaca gctctaatta
     3721 atgaggcatt cttgcgtctt gtcgatcaga cccgggtgca gtggcaaaat cgcgcgcact
     3781 tttttgggat tgcggctcaa attatgagaa gagttctact taactacgcc cgcgatcgcc
     3841 atcgccaaaa acgtggtgga catgctgttc aggtttccct ctccgacgtg gcgattattt
     3901 cggacgataa gtcagctgag ttaattgcgc ttgacgaagc acttgagcga ttggctgcca
     3961 tcgatctaag aaagagtcgt gttgtcgaac ttcgttttta cggcgggctg agtgtggaag
     4021 aaaccgccga ggtcctcggt gtctcaacta taaccgtgaa ccgtgactgg aaaatggcca
     4081 aagcctggct ttccagagag attcgcaatg tcctctgatc aatggcgaag aattgaagat
     4141 ctatttcatg cagcctctca actgcatcct tccgatcaac ttgactatct caagcgcgag
     4201 agtggcaacg atcctgccat cgttgcagaa gtacaatctc taattgaagc gtcgaacgat
     4261 gcgctgtttg aaaccccggc agtcagcctc ggtttgaagg tcttatctaa cgccagcaag
     4321 cctggagccc ttgccggccg cacaattggt cacttcaaga tcattaagct tttgggtgcg
     4381 ggtggtatgg gcgaagttta tctggcaacc gacttgaacc tgcaacgcca ggtagcattg
     4441 aaatttctca atcatgggtt cggaaacgac gagtggttaa gagcgcagtt aattctcgag
     4501 gcacgggcag ttgcgcagct tgagaaccat aacatttgct cgattcacgg actcgaaaac
     4561 gaagatggac aagactttat cgaaatgcag tacgtcgaag gagagacact tcaaaccaga
     4621 ctctcttccg gccctctctc aatcgatctg gcgctcgact ttgcggaaca agttgctggt
     4681 gccttatcgg ctgctcacga gcacgggatt gttcatcggg atgtcaaaac tcaaaacata
     4741 atcgtcacac ctgacgatca aattaaggtg ctcgactttg gattggcgaa gttggttaaa
     4801 ccgatcgagc caacccagag caaaagcgaa cagcatcaga ctggcgcgtc cgggatactg
     4861 ggaaccgttg cttacatgtc gccggaacag gcggaaggcg acgagattga cgcccgaagc
     4921 gacattttca gtttcggcat cgtgttttac gaaatgctca ccggcgaaaa tccttttcgt
     4981 cgagacgaca agtttcaaac acttgaagca atcaaaaacg aacacccaga ccttagcctg
     5041 gacctgccca aacagttgct aaacatcctt ggcggctgtc ttgagaagga tcgggacaac
     5101 agattcaaga gctcgaaggt tttacgatca gagattcaag cattacggcg aagtcgcgag
     5161 ccctcaccct tcacgacgtg gatgcagagt cgcaatttta agtatctggc agcagctgca
     5221 gcggtactcc tggttttcat tctggcaagc tcggcgtttg tatatcacaa gacttcgtcg
     5281 gtccactcga tcgcactact tcagatccaa aaccggagcg gagatgcaaa cctggattat
     5341 ttgagtcagg gattgacgag aagtctgttt gataagttct catatttaac gcggctccag
     5401 gtcaaagcgc cgtcgaccat gccttttgat cgtgaacaga atgttgatct aaaacgagca
     5461 ggacgcgatc tcagcgttga gtcggtgctg tccggcgaga tcctaaaaca cgatcaatcg
     5521 ctcctacttc ggttgagctt gtcggatacc gagaacggcg gaacgaagtg gcaagaggaa
     5581 ttcaacctgg atgccgcgaa catgttcgtc ttacaagacg acatcacgcg taaggttgtt
     5641 tccagtctcg gtttgtggct tatcgggggt gagcgcaagc gattaaacaa acaccagacc
     5701 gataacgacg aagccctgcg caagtacatg ttgggccgcc agttctccgg actcaagaga
     5761 agtagagaaa atatgcagaa ggcgattggt tacttcgaac aggcgataga actggatcca
     5821 tctttcgccg aggcttattc cggcctctcg gattgttatg cggtgatgaa taacgttgct
     5881 aacggcccga tcgcaccttc ggtcgctatg gcaaaggcac gatggagtgc gcaacaggct
     5941 ctcgacctgg acgactctct cccggaggcc cacacttcac tcggtatcat aaacctgcga
     6001 ttcgattgga actgggaaaa ggccgaaagt gaattcaaac gagcaatcga actgaaacca
     6061 gtttatgcgc ccgctcacta ctggtattca aatctgcttt cagccctcgg tcgacatgac
     6121 gaagccattc gtgagagcaa aacgtccagg gatctcgacc catactcttt gcttgctgag
     6181 atgaactacg gccggtcgct ttactacgcc cgtcgctatg ctgaagctga agactacttt
     6241 gctgatttgc taagccagaa cgatcagtat ccttcgttcc aacacatgat ggcgttggtc
     6301 caactgcagc tgggccgtta ccaaccagcc attgagttgc tcgaacagat gcgtaagaac
     6361 gatccactgt atggaaccgc ggcgcttggc tacgcctacg gcaagaatgg acagcgtaac
     6421 gaggcccaaa acatactccg cgcacttgat gaggcttcaa ctcgagaacc ggtgccacca
     6481 catgaaaagg caattgtcta tttaggactg ggtgataagg accaggcctt taagtatctg
     6541 gaccaaagcc tggaagaaag atttgcttcg atggcgtttc ttaatgccga ccctctgtat
     6601 gacgatatcc gttctgacgt tcgcttccgt gagctcgaac aaaaagttgg tctctcacta
     6661 ccgtagcaca cccgattcga cccaatacta aaaaccaaat accgacaatt cggggggtac
     6721 cgacagcaca cttacgaacc tcttagtcca tctagtgtcc tgtgttttat aacaatctag
     6781 cgaagtcatg cggtggactt tcagcgagga tggaaaagaa tctccctgga aaaaagcaag
     6841 aaccgaattg ttagcaaatg ggaagagcct ttggtttcac cccgtggggt gacatgttta
     6901 tagccgacaa ctccagagac agtcccactc cgttcgaagt tctaagaa
//
LOCUS       DASSFP010000330         4534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_238624, whole genome shotgun sequence.
ACCESSION   DASSFP010000330 DASSFP010000000
VERSION     DASSFP010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238624"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFH91_07335"
     CDS             <1..400
                     /locus_tag="VFH91_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6862843.1"
                     /translation="VDGLVLARSAFHHSINYRSVVVFGRAVRIDDRAEKDAALLAFSE
                     QVVPGRWEDVREPTEGELKQTTVLCLSLTEASAKVRTGPPIDDEEDYALPTWAGVLPL
                     QIIPGEPIKDPRLPEGIDVPQYLKNYRREK"
     gene            complement(628..2046)
                     /locus_tag="VFH91_07340"
     CDS             complement(628..2046)
                     /locus_tag="VFH91_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6862844.1"
                     /translation="MAERSINDQLDDAVTEILAGGRPDLSSADPLVVDLTELAIELRG
                     LPRETFYNDLKRQLTGSVEMSSPTQKLATVAIRRFSTYICVSNANAAIDFYVKAFGAK
                     ELMRLAEPDGKIGHAELQIGDSILKISDEYPEYGAVSPQTLGGSPVKLHLQVEDVDEF
                     AQRAVALGARISRPIEDQFYGDRAGQLSDPFGYTWIVASHIKDVSVDEMQEQFDSFNR
                     QKVSKKRREGFHSVTPYLTVKEAPELVDFVKTAFGATETFRTIGSAGGLHCEVRIGDS
                     MVMIGGGPGISEWPTAIHLYIPDVDDAYKRAIAAGAISIFEPADQPYGERSGAVQDRT
                     GNRWYIATTTVPLSEIDRDLHTVTVYFHPPKAQQLIDFLTNAFGAEEIMRSDSGDGVI
                     YHAKVRIGDSVVEMGDARDVSQPMPTAIYLYVDDVDATYEQALKAGGVSVVPPADQPY
                     GDRNAWVKDPFDNLWYIAAPVS"
     gene            complement(2053..2580)
                     /locus_tag="VFH91_07345"
     CDS             complement(2053..2580)
                     /locus_tag="VFH91_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6862845.1"
                     /translation="MNEADERLLIEAAQKDPSRFAELYEQHFHRIYAYIARRVHDRAE
                     TQDLTAHVFQQALANLEKFKWRGVPFSAWLYRIASNAIADYARKLPRETNAENSPIVT
                     ADAESDFEAIERRVRVSRAIDLLPEDQRRVIVMRFADEKSIREIANELGRSEGAIKQL
                     QFRGLENLRSSFSES"
     gene            complement(2668..4428)
                     /locus_tag="VFH91_07350"
     CDS             complement(2668..4428)
                     /locus_tag="VFH91_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6862846.1"
                     /translation="MNRPKRLSGVNVFVTLALILCIVSSQLAQSSRSSDAQSNLRTKR
                     PRLILLIAVDQFRYDYLERFGDLFGPNGFRRLLRDGASWTQSNYDHFPTYTAPGHATM
                     MTGAYPAESGIIGNEWIDRVTGKRVTSVSDETVKLLGGGPNETGSSPKRLLSSTVGDE
                     LRLATNDHSKVIGISIKDRSAILPAGLHANAAYWFSTTSGSMVSSTYYFNELPAWVMN
                     FNKARPADKFFGAKWERLLGEAEYIKRAGPDSQPWETVAKTTGDTNTFPHVITGGATQ
                     PGPVFYNALDFTPFSNDLLVSFAQQAIVNENLGLDDDTDVLTVSFSANDYVGHRFGPY
                     SQESMDITLRVDGQIASLLDFVQAKVGLSNTVVVMTADHGVSPIPEQAAALGLGGGRV
                     KLEDIMGTVRSALTARYNSQNKSPDPTVDYIYKENVGGSFRDTFINGSIYLNYAALKR
                     DGISTDEVEQVIGRAALTVPGVARFFARSQLQRGAVSFTDPIERRVLHGFYPTRSGDV
                     ILLAEPYKYLAETITATHGSPYAYDTNVPTIIMGAGVNPGRYYEPAAPSDIAPTLAAI
                     LGITAPSGSVGRVLLEALKK"
BASE COUNT         1077 a         1160 c         1124 g         1173 t
ORIGIN      
        1 ggttgatgga ctggtactgg ctcggtcggc gttccaccat tcgatcaatt accgctcggt
       61 ggttgttttc ggtcgcgctg tacgaatcga cgacagggct gaaaaggatg cagccttact
      121 tgctttctcg gaacaggtag tacccggccg ctgggaagat gttcgtgaac caacagaggg
      181 cgagttaaag cagactactg ttttgtgtct gtcattgact gaagcgtctg cgaaggtccg
      241 gactggtcca cctatcgacg atgaggaaga ctacgcgttg ccgacttggg ccggagtgct
      301 cccgttacaa atcattcccg gcgaaccgat caaagatccg cggctaccag aaggaattga
      361 tgttccccaa tacctgaaga attaccgtcg cgagaaataa atcgaccgaa tcattgatca
      421 attgtcattt gatatttgag aattgctatt tgtgctttgt gcttcgcact ttgttctttg
      481 tcgtttgtgt tcactgcgat cgataagagt ctcacgctcc aattaataaa aaagaaagtg
      541 agccactaaa cacaaaatag cacttctcaa atatcaaaat cagaattgat caatgattcg
      601 gtctcagcga ttgaccaaat tcgccgctca cgaaaccggg gcagcgatgt accagagatt
      661 atcgaatgga tctttcaccc atgcgttgcg atccccgtaa ggttgatccg cgggaggcac
      721 gaccgacacc cctccggctt tcagcgcctg ttcgtatgta gcatcaacgt catcgacata
      781 tagatagatg gcggttggca tcggttgcga aacatctcgg gcgtccccca tttcaaccac
      841 tgaatcgcca attcgtactt ttgcgtgata gatgacgcca tctccagagt ctgaacgcat
      901 gatctcctcg gcgccaaaag cgtttgttaa gaagtcgatc agttgctgag ccttgggagg
      961 atgaaagtaa acagtgaccg tgtgcagatc tctatctatt tcactaagtg gtacggttgt
     1021 ggtcgcgata taccaacggt tgcccgttcg atcttgaacg gctcctgacc tttcaccata
     1081 gggctgatcg gccggttcaa aaatcgaaat tgcaccagcc gcaattgcac gcttatatgc
     1141 atcgtcgacg tcaggaatat aaagatggat tgcggtcggc cactctgaga ttccaggtcc
     1201 accaccaatc atcaccatcg agtctccaat tcttacctcg cagtgaagac ctcctgctga
     1261 tccaattgtt ctaaaggtct cggtcgcgcc aaatgctgtt ttcacgaaat cgacgagctc
     1321 tggtgcttct ttcacagtaa ggtaaggtgt gacgctgtgg aatccttcac gtcttttctt
     1381 tgacactttc tgtctattga acgagtcgaa ttgttcctgc atctcgtcaa ccgaaacgtc
     1441 cttaatatgg ctcgctacga tccaggtata accgaatgga tcacttagtt ggccagctct
     1501 atcaccatag aattgatctt caatcggccg gctgatcctc gcgccaagcg cgactgccct
     1561 ttgcgcaaat tcgtcaacgt cttccacctg aagatgaagt tttacaggcg agccgcctag
     1621 agtttgtgga ctgactgcgc cgtactcagg atattcgtct gaaatcttca ggatcgaatc
     1681 acctatttga agttcagcgt gtccgatttt tccgtccggc tcagcaagac gcatcagttc
     1741 tttagctccg aacgccttaa cgtaaaaatc gatcgctgca tttgcattcg aaacacagat
     1801 gtaagtagaa aatcttcgaa tcgccaccgt agcaagcttc tgcgtaggac tactcatttc
     1861 aacactcccg gttagttgtc gttttagatc gttatagaag gtttcacgcg gcaggccacg
     1921 taattctatg gcgagctctg ttaggtcgac gaccaacgga tctgcacttg atagatctgg
     1981 acgcccgccc gccagtattt ctgtgacggc gtcgtcaagt tgatcgttga ttgaacgttc
     2041 agccatttgc cttcaacttt cgctaaagct gctgcgaaga ttttccaaac cacgaaactg
     2101 aagttgcttt atcgcacctt cgcttcgtcc gagctcatta gcaatctcac gaatgctttt
     2161 ctcgtctgcg aaacgcatga cgataacgcg ccgttgatct tctggcagca gatcaatggc
     2221 tcgagagaca cgcacacgcc tctcgatcgc ttcaaaatcg ctttctgcgt ctgctgtaac
     2281 gatgggtgaa ttttcggcgt tagtctctcg aggcaatttt cgcgcgtagt cagcgatcgc
     2341 gttcgatgca attcgatata accatgccga aaaggggacg ccacgccact tgaatttctc
     2401 caggtttgcg agcgcctgct gaaagacatg cgcagtcaga tcctgtgtct ctgcccgatc
     2461 atgcactcgc ctggcaatat aggcgtagat gcggtgaaag tgctgctcgt acagctccgc
     2521 aaaacggctg ggatctttct gcgccgcttc aatgagcagt ctctcgtcgg cttcattcac
     2581 ctgattcaat ctggtcctcg cactcgcacc agctttctta ggggataaaa ctggcaagca
     2641 cggaaaaagt tacggcggtt cgatgttcta ttttttgaga gcttcgagca aaacgcgtcc
     2701 tactgaccca cttggagcag taattccaag gatcgcagca agcgttggcg cgatatctga
     2761 aggggctgca ggctcgtaat atcgacctgg attaactccc gcgcccatta tgattgttgg
     2821 tacattcgtg tcgtaagcgt aaggggaacc atgcgtcgct gtaatagttt ctgcaaggta
     2881 tttgtatggc tccgccagta gaatcacatc gccgctgcgc gtcgggtaga agccatgtag
     2941 cacacgtcgt tcaatcggat cggtaaaaga caccgcacct ctttgcagct gactacgagc
     3001 aaagaaccgt gcaactcccg gaacggttaa ggcggcgcgg ccgatcacct gttcgacctc
     3061 atctgtcgag attccatcgc gttttagagc tgcatagttg aggtagatcg agccattaat
     3121 gaaagtatct ctgaaggagc ctccgacatt ctctttgtag atgtaatcga ctgtcgggtc
     3181 gggcgatttg ttttgactgt tataacgggc agtcagtgcg gatcgaaccg ttcccataat
     3241 gtcttccaat ttgactcgac cacctcccag acccagcgcg gccgcctgtt cgggaattgg
     3301 cgagacgccg tgatcggcgg tcatcaccac gacagtgttc gatagtccta ccttcgcttg
     3361 aacaaaatcg agtaggcttg caatttgtcc atcgacccgc agagttatgt ccattgactc
     3421 ctggctatat ggcccaaagc ggtggcccac atagtcgttg gctgagaaac taacggtcag
     3481 tacgtcggta tcgtcatcaa gtcctaagtt ctcattcaca atcgcctgtt gagcaaacga
     3541 caccagcaag tcattcgaaa aaggcgtgaa gtccagcgcg ttgtaaaaga ctggtccggg
     3601 ctgcgtggcg ccgccagtga ttacgtgagg aaaagtgttt gtatctcccg ttgtttttgc
     3661 cacggtttcc cacggctggg aatccgggcc agcccgcttt atatactcag cttcacccag
     3721 caggcgttcc cattttgctc caaaaaattt atcggcgggt cgtgccttgt tgaagttcat
     3781 cacccaggcc ggcaactcat taaagtaata ggtggatgaa accatgctgc cggaagtggt
     3841 actgaaccag tacgccgcgt ttgcgtgaag gccggctggt aagattgctg accgatcctt
     3901 aattgagatt ccaatgacct ttgagtgatc gttcgtagcg agtcttaatt cgtctccgac
     3961 agttgaagac agcaaacgct tcgggcttga tccggtctca tttggaccac cgccgagtag
     4021 tttgaccgtt tcatctgata cgctggttac tcttttaccg gtaactcgat caatccactc
     4081 gttcccaata attcctgact cagcagggta cgcgcctgtc atcatcgttg catgtcctgg
     4141 cgccgtatag gtagggaagt gatcgtagtt tgattgggtc cacgatgcac catctctcaa
     4201 cagtcggcgg aatccgtttg ggccaaacag atctccgaag cgttcaagat agtcgtaacg
     4261 gaattggtca acggcgatta gcaggatgag acgtggtcgc tttgttctta aattgctttg
     4321 cgcatccgaa cttcgacttg attgagcgag ttgagaactg acgatgcaaa gaattagcgc
     4381 aagcgtgaca aagacgttca cgccggagag acgtttgggt cgattcatgc cgcgcatcgt
     4441 aaccgaagaa gcctggaaaa cagaactgcg aggttatagg acctataaga cccataggac
     4501 ctattccccc ggcagaagag gttgagtggt gcaa
//
LOCUS       DASSFP010000331         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_393849, whole genome shotgun sequence.
ACCESSION   DASSFP010000331 DASSFP010000000
VERSION     DASSFP010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393849"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFH91_07355"
     CDS             complement(<1..557)
                     /locus_tag="VFH91_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862847.1"
                     /translation="MALDTLRANKLRSSLTILGVSVGVVTVIFMVSIIQGLNKAFADQ
                     IESLGSNTIFVSKFAPSFGRPPGPDEIHRKDLTMDDAEALRKEASSIVGVSPIHRMIA
                     ATIRYQDKQTDTPILLGVTPYYEFVHSQYVASGRFVSDIDMQDRSNVVIIGVDVKQAL
                     FPYEDPVDKEVRINGNPYRVIGVMEP"
     gene            complement(721..2058)
                     /locus_tag="VFH91_07360"
     CDS             complement(721..2058)
                     /locus_tag="VFH91_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6862848.1"
                     /translation="MKRGGDQAAKPRRFIPAVIPFTSRTNSSSAFRLTAIWEAFRVAT
                     ASLRANKLRTSLTLMGIVVGVTAVIAVVTIIKGLDKTVAQTFSSQGSTVFTISKNPQI
                     IKSREEFVKFNKRKDVTHDDAGAIARLCSVCWRVGIAANAIETVKYADQKAENVRIRG
                     VSPTTMFDIDGVTIDTGRIWTESEGESGQDICVIGPDMLKNLFNDAPADRAMGQEIRI
                     AGRPYRIVGVLEPLGSIFGFSRDNVVYIPYSTYQKAYGARRSLVVFIQVPTPEQLEVA
                     EDQVRTVMHNRRGHTADNPDDEGFALETQDVFLNLYGSATSNIYIVTIGVAAVSLVVG
                     GIVVMNIMLVSVTERTKEIGIRKAIGARRKDILTQFLIEAVTVTAVGGAIGVATGFGL
                     AYVISALIGFPLLISVASAVLGVGVSSIVGIVSGLWPAWRAAKLDPIEALRAE"
     gene            complement(2071..2832)
                     /locus_tag="VFH91_07365"
     CDS             complement(2071..2832)
                     /locus_tag="VFH91_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862849.1"
                     /translation="MSDVTLVSSPVENVIKPKTEKNGHDPSVLISMRNIWKTYQMGTE
                     SVHALHGVSFDIPRGEYIAIIGPSGSGKSTLMNLIGCLDTPSQGEYWLNGKNVSEMDD
                     DELARIRNQEVGFVFQTFNLLARATALHNVELPLIYGGVSSGQRHEMAKKALESVDLA
                     ERMMHKPNELSGGQRQRVAIARALVNHPSILLADEPTGALDTHTSMEIMDLFERLHSE
                     GNTIIVVTHEPDIAARAHRVISIRDGQIEKDHRIR"
     gene            complement(2833..>3785)
                     /locus_tag="VFH91_07370"
     CDS             complement(2833..>3785)
                     /locus_tag="VFH91_07370"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6862850.1"
                     /translation="FETAKISLETARANLEAQRIAVKEATERANQQKIAVRDAQTAIK
                     SSEMRANQQAAMLRGQTSQRSKATQLSPLNGVVADIPTRVGEYAVAGLSTTPLMTIAD
                     MSQINVEVNVDETEINNVEVGQQAKVKVDALGDKEIKAVVTQKNPLAIAKSDQTGGLS
                     NRVNVQEAKEFKVTLQLQELPDEVQTSLRPGMSSTATITTKTKNNVLAVPLESIVEKS
                     PTPNASPSPSIAGNTPTPVSSDKPKPQKGVYLIEGNKVRFVEVTTGITGEADIEITSG
                     LKPTQEVVRGPSRILKTLKDGMTVKRQTKKSANVNGNEGS"
BASE COUNT          840 a          987 c          932 g         1026 t
ORIGIN      
        1 ggctccatca cgccaatcac ccgataggga ttgccattga ttctgacttc tttatcgact
       61 ggatcctcgt aaggaaataa tgcttgcttc acgtcgactc cgatgattac gacgtttgaa
      121 cggtcctgca tatcgatgtc ggaaacgaac cgtccggatg caacatactg agagtgtaca
      181 aactcgtagt aaggagtcac tccaagcagt attggtgtat ctgtctgttt atcctggtat
      241 cggatagttg ccgctatcat tcggtgaatc ggcgagacac caactatcga tgatgcttcc
      301 ttacgtagtg cttcggcatc atccattgtt agatcttttc gatggatttc gtctggacca
      361 ggtggacgtc caaagcttgg cgcaaatttc gaaacgaaga tcgtgttgga gccaagcgac
      421 tcgatctgat cggcaaacgc cttattcagt ccctggatga tcgagaccat gaagatgact
      481 gtcactactc cgacgctgac gccgagaatt gtgagagacg agcgcagctt attcgctcgc
      541 aaggtatcga gtgccatgag ggccgattct tttatgtcgt caagcctcat tttggagtgc
      601 gccggcccgg cggcgctttt cagtcaccta cctagctttc tacttcaatt ctaaataact
      661 ttcttagcga catcgaacta acgatataac aatgcggcgg caggccgccg cactccaagg
      721 ttactccgct cgtagtgcct caatcggatc gagttttgct gcacgccacg cgggccaaag
      781 tccactgaca atgccaacaa tcgacgagac gccaacgcct aacaccgcag acgcaacgct
      841 tatcaagagc ggaaaaccga tcagagcgga gatcacgtaa gccaatccga aacctgtggc
      901 tactcctatt gcgccaccaa ctgcagtgac tgtcactgcc tcgatcaaaa actgagtcag
      961 aatgtctttg cggcgcgctc caatcgcctt tctaatacca atctcttttg ttcgttcggt
     1021 caccgacacc agcatgatat tcatcaccac gattccaccc accaccagcg aaactgctgc
     1081 aacaccaatt gtcactatat agatgttaga agttgccgaa ccgtagagat tcaaaaacac
     1141 gtcctgcgtt tccagagcga atccctcgtc gtccggattg tccgcggtat gtcctcgacg
     1201 gttgtgcatg accgtacgga cttgatcttc tgctacttca agttgttcgg gagtcggaac
     1261 ctggataaag acaaccagcg aacgtcgagc accgtaagct ttttgatacg tcgaataagg
     1321 aatgtagacc acgttgtcgc gcgaaaagcc aaatatcgat cccagcggtt ccagcacccc
     1381 gacaatccgg taaggtcttc ctgcgattcg aatctcctgg cccatagccc gatctgcggg
     1441 agcgtcattg aacagattct tgagcatgtc tgggccaatg acgcaaatat cctgacccga
     1501 ctcgccttcg ctttctgtcc agatacgccc ggtatcgatg gtaactccgt cgatatcgaa
     1561 cattgttgtt ggactgacac cgcgtatgcg tacgttttca gctttctgat ctgcatactt
     1621 cacagtttcg atggcgtttg cagcaatgcc gacgcgccag catacactgc acaagcgagc
     1681 gatcgcgccg gcatcgtcgt gcgtgacatc tttccgttta ttgaatttga cgaactcttc
     1741 tctcgactta attatttgag gattctttga aatagtgaag acggtcgagc cttgagatga
     1801 aaatgtttga gcaactgtct tgtcgagtcc ttttatgatc gtgacgactg caatcactgc
     1861 ggtaacgccc acgactattc ccataagcgt cagcgaggtt cttagcttat tcgcgcgcag
     1921 actcgcagtt gcgactcgaa acgcttccca gattgctgtg agacggaatg ccgaagaaga
     1981 gttagttcgg gaggtaaagg gaataacggc agggataaag cgacgtggct ttgctgcctg
     2041 gtcgccacct cgcttcatgg acttgatctc ctacctgatt cgatggtctt tctcaatctg
     2101 accgtcgcga atggaaatga cccggtgcgc gcgtgcggct atgtcaggtt cgtgcgtgac
     2161 aacgatgatc gtattacctt cggagtgtag tcgctcaaag aggtccataa tttccatact
     2221 ggtgtgcgta tctagcgcgc cagtaggttc gtcggccagc aatattgatg gatgattgac
     2281 gagagctcga gcaatcgcga cacgctgtcg ttgaccgccc gatagctcgt tcggtttgtg
     2341 catcatgcgt tcggccagat ctaccgattc gagagccttc ttggccattt catgtcgttg
     2401 acccgagctt acgccgccgt agatcaacgg cagttcgacg ttatgaagcg cagtagctcg
     2461 agccaacaag ttgaacgttt gaaaaacaaa cccaacttcc tgattacgga tgcgagccag
     2521 ttcgtcatcg tccatctcgg acacgttttt gccgttgagc cagtactctc cctgagacgg
     2581 tgtgtcaaga catccgataa ggttcatcag ggttgattta ccagaccctg atggaccaat
     2641 tatcgcgatg tattctcctc gaggtatatc gaacgaaaca ccatgaagtg cgtgtactga
     2701 ctctgtaccc atctggtagg tcttccagat gttgcgcata gaaatcagca ccgacggatc
     2761 gtgtccgttc ttttccgtct tgggcttgat gacgttctcg actggggatg atacgagtgt
     2821 aacgtcgctc atttagctgc cctcgtttcc attcacattg gctgatttct tggtctggcg
     2881 tttgaccgtc ataccatctt tcagggtttt gagtatccgg ctgggtccac gtacaacttc
     2941 ctgagtgggt tttagaccac ttgtgatctc aatgtcagct tcgccggtta tacccgtggt
     3001 gacctcgaca aagcggacct tgttcccttc tatgagatag acaccttttt gcggctttgg
     3061 tttgtcgctg gagactggtg tcggtgtgtt gcccgctata gatggtgatg gcgacgcatt
     3121 aggtgttggc gacttttcga caatcgattc aagcggaacc gcaaggacgt tgttcttcgt
     3181 cttcgtggta atcgttgcgg tcgaactcat tccaggtcgc agagaagtct gcacttcgtc
     3241 tggcaattcc tgaagctgga gagttacttt gaactccttt gcttcctgca catttactcg
     3301 attcgagagc ccgcctgttt ggtccgactt ggcaattgcc aatggattct tttgtgtcac
     3361 aaccgccttg atctctttat cgcccagggc gtctactttc accttcgcct gttgtcccac
     3421 ctcgacgtta ttgatctcgg tttcgtcaac gtttacttct acgttgatct gggacatgtc
     3481 cgcgatagtc atcagaggtg ttgttgataa accagcgacg gcatattcac cgacgcgagt
     3541 gggaatatcg gcgacgacac cattcaacgg agaaagttgt gttgctttgg accgttggct
     3601 tgtttgacct cgcagcatgg ctgcttgttg gttagctctc atttccgagg acttgatcgc
     3661 agtttgtgca tccctaacag caatcttctg ctggttagct cgttcagttg cttctttaac
     3721 ggcaatccgc tgagcttcaa gattcgcccg ggctgtttcg agtgaaatct tcgcagtttc
     3781 gaaac
//
LOCUS       DASSFP010000332         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_394373, whole genome shotgun sequence.
ACCESSION   DASSFP010000332 DASSFP010000000
VERSION     DASSFP010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394373"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFH91_07375"
     CDS             <1..242
                     /locus_tag="VFH91_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862851.1"
                     /translation="DVAYFAEKPFGPGPTASLPSRVEITRPQDQYKLSITYQDPTSVE
                     LNRDYPPQAFVLENKWQLPEVDLDAQNSRKVTANH"
     gene            417..1109
                     /locus_tag="VFH91_07380"
     CDS             417..1109
                     /locus_tag="VFH91_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862852.1"
                     /translation="MRTILQAENLVKVYRIGKVDVPALRGVSLEVEEGEFLAIMGPSG
                     CGKSTMLHLLGGLLTPTSGRIVIDGDDITSASDAKRTAVRRKKIGFVFQRFNLFPTLT
                     AEGNLRLAERIHGNGGEDPGRRREVLRLLGLEDKLHHKPLELSGGEQQRVALARAVVT
                     RPAIVLADEPTGNLDSENSALVLNMFQELNYRFNQTIIMITHNPEAAAMCKRIVQMRD
                     GHIVEPSPGVRL"
     gene            1196..1543
                     /locus_tag="VFH91_07385"
     CDS             1196..1543
                     /locus_tag="VFH91_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862853.1"
                     /translation="MKKVRGLLTTLKKIFFWNYARNTWQWDILCVVILVFIFLTPKSW
                     FSGGERPVDVEHPSRIATLVLSPEVIENEEDKTKIEQRVRLVTGRSEAQVLAVRKMVG
                     SDGKTLSYEVDIR"
     gene            1607..>2119
                     /locus_tag="VFH91_07390"
     CDS             1607..>2119
                     /locus_tag="VFH91_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862854.1"
                     /translation="MKRFVSLVLVPAFMLGAAVLAAPKATKAQGAGLVSSVLNRLEKN
                     RASLKSLKAGITVEKYNSQIGTNDKLEGVVTYLPVSGRQASLRIDWTRPTAEVLAVAK
                     GRYQLYRPRLGIVYTGAQNKAPQSSGLIDMMYMSRQQLETRFQPLQDVREETLGGGVS
                     TIHLTLIPKGN"
BASE COUNT          547 a          504 c          552 g          516 t
ORIGIN      
        1 ctgatgtagc gtactttgcc gaaaagccgt ttggccctgg tccaacagct agtcttccca
       61 gtcgtgtcga aataaccaga cctcaagatc aatacaagct tagtatcact tatcaggatc
      121 ccacatcagt cgaattaaac agagactatc cgccgcaagc gtttgtgctg gagaacaagt
      181 ggcaactacc ggaggttgat ctcgatgcac aaaactcgcg caaggtaact gcaaatcatt
      241 aaccctgaca accggctttc caatcgtttc ctaccacgct gctagataac aacgttcaga
      301 tgtattaaca tctgtcgctt gagttttaca tcaacttcat ttgcaggtga gcccgctgtt
      361 cgttagaacc tttcctggcc gtgcggataa aaattgcgaa cggccgtcac aattcgatga
      421 gaacaattct acaagctgaa aatttggtga aggtctatcg tatcggcaag gtcgacgtgc
      481 cggcattgag aggggtttca ctcgaggtcg aagaaggcga atttctggca atcatgggtc
      541 cttccggttg cgggaaatca actatgttac atctccttgg cggactctta acccccacca
      601 gcggcaggat tgtaattgat ggcgacgaca tcacgtcagc ctcagatgct aaaagaaccg
      661 ctgtccgacg caagaaaatt ggctttgtat tccaaagatt taatctgttt ccaaccttaa
      721 ccgctgaagg aaatctccga ctggcggagc gaattcatgg caacggcgga gaggatcccg
      781 gccgtcgtcg agaggtgttg cgcttacttg gtttggaaga caagcttcat cacaagcctc
      841 ttgagctctc gggtggcgaa caacagcgcg ttgctcttgc gcgagcggtc gtgaccaggc
      901 cggccatcgt cctggcagat gaaccaactg gaaatctcga ttccgaaaat tctgctcttg
      961 tgcttaacat gttccaggaa ttgaactacc gcttcaatca aaccatcatt atgattaccc
     1021 acaatcccga agctgcggca atgtgtaaac ggattgtgca gatgcgtgac gggcacatcg
     1081 ttgaaccctc gccgggagtc cgcttgtgat aaatccgttt acatggcgta gtctaaacgc
     1141 taaaatcctt ctgtaccctc cagccacgga gtctcgtggc tgatcacttg caagcttgaa
     1201 aaaggtgcga ggcctgctaa cgacgctaaa gaagattttc ttctggaact acgcaagaaa
     1261 tacctggcag tgggacatac tttgtgtcgt catcctcgtg tttatctttt tgacgccaaa
     1321 gagctggttt tctggtggcg aacggcccgt agatgttgag catccaagcc gtattgccac
     1381 cctggtgctg agcccggaag ttattgaaaa cgaagaggat aagaccaaaa tcgaacagcg
     1441 tgttcggttg gtgactggac gttcagaggc gcaggtgttg gctgtccgga aaatggtcgg
     1501 ttcagacggg aagactctca gctacgaggt tgacatccgg tagtcttccc ctataattgc
     1561 tcggtttttt gcgagccgag ggattagcaa tgatgaagga gtttgtatga agagatttgt
     1621 ttccctggtt ttggttccgg cattcatgtt aggtgcagcg gtattggccg cgccaaaggc
     1681 gaccaaggcg cagggcgctg gtctagtaag ttcagtgttg aatcggctgg aaaagaatcg
     1741 ggcgagtttg aagtcactca aagcaggcat aactgtagag aagtacaatt cacaaattgg
     1801 cacaaacgat aaactagaag gtgtggttac ttacctacct gtatcaggtc gtcaggcttc
     1861 gctgcgaatc gattggacac gtccgaccgc ggaagtcctg gcagtcgcga aggggcgcta
     1921 tcagctgtac aggccaaggt tgggcattgt ttacactggg gcacaaaaca aggcacctca
     1981 gagtagcggt ctaatcgaca tgatgtacat gtcaagacag cagttagaaa ctcgattcca
     2041 accactgcag gatgttcgcg aagagacatt gggtggtgga gttagcacga tccatctcac
     2101 gcttattccc aaaggaaat
//
LOCUS       DASSFP010000333         5957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_394452, whole genome shotgun sequence.
ACCESSION   DASSFP010000333 DASSFP010000000
VERSION     DASSFP010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394452"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..2563)
                     /locus_tag="VFH91_07395"
     CDS             complement(362..2563)
                     /locus_tag="VFH91_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862855.1"
                     /translation="MIINHVFRLSTLALLLISSSIVYAGQPVVWETSARADLLKGDAR
                     GVSISDTGVLMLSPKLTELFNTQQTYVWSTAVDNAGNVFVGTGHDGKIFRIAANGTGS
                     LLYDAAELDVTALAVGRDGSLYAGTSPDGKVYRIGADGKADVYFDPDDKYIWSLAVMP
                     DGSLAVGAGDSGKIYRVRAAGAKPDVSLLVSTNQTHVISLAVTPQGDLIAGTDPGGLV
                     LRISPEGKTFALFDTQLREIHALAPAADGSIYALALSDAAAGNKGPTAPVVTSQPSEG
                     STPTTTVTITAIDETGAPLQAQPTATRSRNDVSNARSAVFRILPDGATDVVWSSTTIT
                     AFSITPALTGGSVLIGTADKGRIYSVTNDGRDTLLLQSPEGQISSMIVKGNQIYAASS
                     NQGKLFRFGNDLVSEGSYESPVRDAKLIATWGRVWWRGAGNIEVQTRTGNGERPDATW
                     SDWSSSVKNPDGNQISSPRARFIQWRTVVRSGAGSPTWLEDVSVAYLPRNVAPEVLTI
                     TALPIGVGLQQIAQVAIDPNVESSGLDPSVFGPVAQVPPRRFYQRGARSFQWQAEDRN
                     GDTLEYSIYYRPFNEQTFRLLKDKLRDNFFTIDGATLADGRYVIKVVASDGPDNPLGQ
                     KLTGERLSEPVDIDNTPPVVKVMDQPQAGRDNVRVVFSADDATSKIRKADISIDGATW
                     LPIFPDDGIADSGHETFTVDAGALTPGEHTISLRSFDTSGNVGTASVTVRK"
     gene            complement(2570..3592)
                     /locus_tag="VFH91_07400"
     CDS             complement(2570..3592)
                     /locus_tag="VFH91_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET6862856.1"
                     /translation="MFSDRYSRQALFDGIGIEGQTKLLTSRVLIIGVGALGTSQAELF
                     ARAGVGRVRIVDRDFVELSNLQRQSMFTEQDAQQRLPKVVAAENHLREINSEITIESE
                     IADVNHSNVESLISDCDVVIDGTDNFATRYLINDACVKHNIDWIYGAAVGSYGVTMTI
                     LPYQTPCLRCIFEEAPAPGSSPTCDTAGVIMPVINVIAAIQVTEALKLLTGQRSALHQ
                     SLMQFDVWENQWRKINPGNRRADCETCGLNLFETLATTPAETAAILCGRDAVQISPQR
                     PGLIDFKALARRLSGAGEVKFNEYLLRFTTGDFELTVFRDARSIIRGTSEIATARSLY
                     AKYIGN"
     gene            complement(3934..4317)
                     /locus_tag="VFH91_07405"
     CDS             complement(3934..4317)
                     /locus_tag="VFH91_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6862857.1"
                     /translation="MSSDDTNSGISVSDEQALEHGKDESTVDVRLNSLRETVLLLLDE
                     VESLSISVPVDIKNGARFSDEVRQFEVNLIRTALGRTSGSQIRAAKLLGLKPTTLNAK
                     IKRYNITPTGYHAQGDRSEAHQNAA"
     gene            complement(4546..5325)
                     /locus_tag="VFH91_07410"
     CDS             complement(4546..5325)
                     /locus_tag="VFH91_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HET6862858.1"
                     /translation="MTDATRIRALVIDDEPLAREMIREMLSSDPSIEIIAECANGREA
                     VAAIKEQSPDLIFLDIQMPELGGFEVLESLEPGKAPYVIFATAYDQYAVRAFEVHAFD
                     YLLKPFDNERFEVAWQRAKNQIQSDRSGQRDQHILALLEELKTGPSYLERLVIKAGGR
                     VFFLSVDDVVCIEAEGNYVRVYDNNKSYLLRETISGLEEQLDPKQFLRIHRSAIVKID
                     KIKEMQPWFHGEYRILLTNGKQLTLSRNYRANLQEAVGNSL"
     gene            complement(5658..>5957)
                     /locus_tag="VFH91_07415"
     CDS             complement(5658..>5957)
                     /locus_tag="VFH91_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862859.1"
                     /translation="PNLILQPIVENALRHAVARSNSGRVEISAAPRNGSVRIQIKDNG
                     PGLDVEHKAMSRSGKGVGLANTQARLLGLYGDAAHFELTNRPTGGLTVVLEIPRR"
BASE COUNT         1485 a         1562 c         1443 g         1467 t
ORIGIN      
        1 taagtatcga cggcgcgacc tggcttccga tatttccaga cgacggaatt gcggatagtg
       61 gtcacgaaac attcaccgtg gatgccggag ctttaacgcc cggggagcac acaatttcct
      121 tgcgctcatt cgacaccagt ggcaacgtag gaacagccag tgtgacggtg aggaaatagt
      181 tcgggaagaa ggaagccatc ttccatatat cattttccat ttctcacttt ccatttggtt
      241 tcgccatttg acttcggcag tcagatattg gccatatatt cgcgaccagc atctcggccg
      301 actagatcaa acgaaaaatg atatatggaa aactatggag aatggcttcc ttcttcccga
      361 actatttcct caccgtcaca ctggctgttc ctacgttgcc actggtgtcg aaagagcgca
      421 aggaaattgt gtgctccccg ggcgttaaag ctccggcatc cacggtgaat gtttcgtgac
      481 cactatccgc aattccgtcg tctggaaata tcggaagcca ggtcgcgccg tcgatactta
      541 tatcggcctt cctgatcttg ctcgtagcgt catcggccga aaatacaacg cggacattgt
      601 cgcgaccagc ctgaggttgg tccatcactt tcaccaccgg cggagtgttg tcgatatcca
      661 caggctcgct caatctttca ccagtaagct tctgacccaa agggttgtct ggtccatccg
      721 acgcgacgac tttgataacg taccggccgt cagccaaagt tgcgccatca atcgtaaaga
      781 agttgtcgcg cagtttgtct ttcaacaacc tgaaagtttg ctcgttgaat gggcggtagt
      841 aaattgaata ttcaagtgtg tcgccgtttc ggtcttcagc ctgccattgg aaggatcgag
      901 cccctctctg gtaaaaccgc cgtggtggaa cctgggccac tggaccaaat accgaagggt
      961 ctaatccaga cgactcgacg ttaggatcaa tcgcaacctg ggcgatctgc tgtaaaccca
     1021 cgcctattgg aagagcagta attgtcaaaa cctcaggagc tacgtttcgc ggaagatatg
     1081 caacgctgac atcctccagc cacgtgggcg atccggcgcc agatctaaca accgtacgcc
     1141 attgaataaa cctcgcacgc ggacttgaaa tctgattgcc gtcaggattc ttcacactcg
     1201 aactccagtc actccaggtc gcatccgggc gctcaccatt tcctgttcta gtctgtactt
     1261 caatgttccc tgccccgcgc caccacactc ttccccaggt agctatcaac ttggcatctc
     1321 gcacaggcga ctcgtaactg ccttcagaga ccaggtcgtt tccaaaccga aagagtttgc
     1381 cttgattgct tgaggccgca taaatttgat tgcctttaac aatcatcgac gaaatctgac
     1441 cttcaggcga ctgcaataac aaagtatccc taccatcatt cgtcaccgag tagatccgcc
     1501 ccttgtcggc agtgccaatc aagacactgc caccggtgag agcgggcgtt atagaaaacg
     1561 cggtaatggt cgttgaactc cagaccacgt ccgtagctcc atccggcaag attctgaata
     1621 ccgcagagcg agcatttgaa acatcgtttc gacttcgagt tgccgttggc tgggcttgca
     1681 agggcgcgcc cgtttcatca attgccgtga tcgttacagt agttgttgga gtcgacccct
     1741 ccgacggttg agatgtcact acgggagcgg tagggccttt gttcccagcc gccgcatcac
     1801 tcaacgcgag cgcgtaaatc gaaccatccg cagcaggagc cagagcatga atctctctca
     1861 attgggtgtc gaagagtgca aacgtctttc cctccggcga aattctcagc accaaacctc
     1921 ccggatcggt gcctgcaatt aaatcccctt gaggcgtgac ggcgagtgag atcacatgag
     1981 tttgattcgt gcttaccagc aacgacacat cgggtttagc gcctgcagct cgaacgcgat
     2041 aaatctttcc actatcgcca gctcccacgg caagtgatcc gtcaggcatt acggcaagtg
     2101 accaaatgta cttgtcatca ggatcgaaat agacgtctgc cttgccatcg gctccgatac
     2161 ggtagacttt tccatcgggc gacgtacctg cataaagaga accatcgcgg cccaccgcca
     2221 acgcagtgac atcgagttca gccgcatcat acaagagact tccagtaccg ttcgcagcga
     2281 tgcggaaaat ttttccatcg tggccagtgc caacaaaaac attgcctgca ttatctacgg
     2341 cagtggacca cacataagtt tgttgcgtgt tgaataactc ggtcagtttt ggcgacagca
     2401 ttaacacgcc ggtatcgctg atggaaacgc ctcgagcgtc gccctttaaa agatctgcac
     2461 gcgcacttgt ctcccaaaca acgggctggc ctgcataaac aatggaggag gaaataagga
     2521 gtagtgctaa cgtggagagt cgaaaaacat ggttaattat catcttcgat tagtttccta
     2581 tgtacttagc gtaaagggat cgagcagttg ctatttcact tgttcctctg atgatagatc
     2641 tggcatcgcg aaaaacggtg agttcaaaat cacccgtagt aaaacgtaag agatattcat
     2701 taaatttcac ttcgcccgca ccactaagtc gtcgggctag agctttgaag tcaatcaatc
     2761 caggacgttg aggagagatc tgaacagcat cgcgtccaca aagaatagcc gccgtctccg
     2821 ctggagtcgt agcaagagtt tcaaacaaat tcagtccgca tgtttcacag tccgcgcgtc
     2881 gatttccagg atttattttt ctccactgat tttcccaaac gtcaaattgc attagtgact
     2941 gatggagcgc tgaacgttgt ccggttaaca gtttgagagc ctctgttacc tgaattgctg
     3001 caatcacgtt gatgacaggc attatgactc cggccgtgtc gcaggtggga gaactgcccg
     3061 gcgccggagc ttcttcaaag atgcagcgca aacacggtgt ttggtacggg agaattgtca
     3121 ttgtcacgcc gtacgagcca actgccgcac cgtaaatcca atcgatgttg tgcttaacgc
     3181 aggcgtcatt tattaggtac cgcgttgcga aattatcagt cccgtcgata actacgtcgc
     3241 agtccgagat gaggctttca acgttggaat gattaacgtc agcgatctct gattcgattg
     3301 ttatttcgga attaatctcg cgtaggtggt tttcagcggc caccaccttc ggcagacgct
     3361 gttgcgcatc ctgttcggtg aacatcgact gacgttgcag gttggacaac tcgacaaagt
     3421 cacgatcgac gatacggact cgacccacac cagctcgcgc gaacaattcc gcttgagacg
     3481 tccctaaagc tccgacaccg attatcagga ctcgagatgt tagtagtttg gtctggccct
     3541 caataccaat tccatcaaag agagcctgcc tgctgtaccg atcgctaaac acgatcccag
     3601 tataacagcc cgtggatcag gtcagtacct cacgacgagg tagtgctgac tagtgtcctg
     3661 tttctaaagt tcgttgaaag aaacgaaaca gtttggcgaa caaggttgct ggtaaagggc
     3721 agggtcgcga cgcaaactta actaataata acgggcaagt tggatctatt gatcgattgg
     3781 cagtaacaaa tcggctcttg ctttttccag ggagattctt tcacagcctt gctgaaagtc
     3841 caccgcatga cttcgctagt tactattcaa gtaaaagaaa taaggccgag attattctcg
     3901 gccttatctc acgaagcaac ccttcaacca actctacgct gcgttttgat gagcttcaga
     3961 gcgatcaccc tgcgcgtggt atccggtggg cgtaatgtta taacgcttga tcttggcgtt
     4021 gagcgtcgtg ggcttaagtc ccagcagctt tgcggctcgg atctggctac ccgatgttct
     4081 gcccagtgcc gttcggatca gattgacttc aaactgtcgc acttcatctg agaatctcgc
     4141 tccattctta atgtcaactg gaaccgagat gctgagggac tcaacttcgt ctagaagaag
     4201 aagaacagtt tctcgcaacg agtttaacct gacatccacg gtcgattcat ccttgccgtg
     4261 ttcaagcgct tgctcatcgc tcactgaaat ccctgagttc gtatcatcac tactcattac
     4321 ctgacctcct gaaacgcggt gctggtccaa ccatggggag cctcaagagc gcggtttaaa
     4381 ccctatacca gttcgttgtc ccatcctgcc ttgctgagct actggcatat ggcatagcca
     4441 cgatgctaat cctgccacaa tgctctcgag gacgtatcac ctgtgtgaca gaacggctaa
     4501 tagttgtgat ggaaaggcca acttcatgga caaaaagcca gaatattaga gagagtttcc
     4561 gacggcttcc tgaaggtttg cgcgatagtt acggctgagg gtcaactgtt tgccgttcgt
     4621 gagcaggatg cgatattccc catgaaacca gggctgcatt tctttgatct tgtcgatctt
     4681 gacaattgct gagcgatgaa tgcgtaggaa ttgttttgga tctagttgct cctcaagtcc
     4741 actgatagtt tcgcgcagca ggtagctttt attattgtca tagacccgaa cgtaattacc
     4801 ttcggcctct atacaaacaa cgtcatcaac actaagaaag aaaactctgc cgccggcctt
     4861 gataacaaga cgctcaaggt aacttggtcc agtttttaat tcttcgagga gcgcgaggat
     4921 gtgttggtca cgctggccgc tgcgatcaga ttgaatctga tttttagcgc gttgccatgc
     4981 tacctcgaaa cgttcgttat cgaacggctt aagtagatag tcgaaagcgt ggacctcgaa
     5041 ggcacgcact gcatattgat cgtaagcggt tgcaaagatg acatacggag ccttgccggg
     5101 ctcaagcgac tcgagtactt caaagccgcc aagctccggc atctggatgt ccagaaagat
     5161 aagatcaggc gactgttcct tgatcgcggc aacagcttcg cgaccattgg cgcactcagc
     5221 gatgatctca atacttggat cgctggacaa catctcacga atcatttcgc gagcgagcgg
     5281 ttcgtcatcg atgaccaacg cccgaatacg tgttgcgtcc gtcacctagc ctccctgggt
     5341 actgtgcttc taacttaacc gcctgtgcaa agtaaggtcg cacataaaat gaacaccgct
     5401 gttcaataac ttgtggcgaa aaccaaagta ctgtgtgacg gtaattccga aagcagcggt
     5461 cgctcattgt tacgtttgct acgcggtcaa tgtttcggac aattccacaa aacggaattt
     5521 tagttttcaa acaagcaaga aattgtacga ccgtacaagt tatcgttgtt ttggaatgcg
     5581 atcttaaata atagaggaga gttggaagag gtcagtagct agataacaaa gcgccgccgg
     5641 gccggcgcac tccagagtta gcggcgggga atttcgagga cgacggtcag gcctcccgta
     5701 ggacgattgg tcagctcaaa gtgagctgca tcgccataaa gtcccaacag gcgagcctga
     5761 gtattggcaa gtccaacacc cttcccactg cgcgacattg ctttatgttc aacgtcaagc
     5821 ccaggcccgt tgtctttgat ctgaattcga acggaaccat ttctcggcgc cgcactgatt
     5881 tcaaccctgc cggaattact tcgcgcaact gcatggcgca acgcattttc aactattggc
     5941 tgcaaaatca gattcgg
//
LOCUS       DASSFP010000334         3918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1866302, whole genome shotgun sequence.
ACCESSION   DASSFP010000334 DASSFP010000000
VERSION     DASSFP010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866302"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFH91_07420"
     CDS             <1..868
                     /locus_tag="VFH91_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6862860.1"
                     /translation="SFYFVEGGTGRPLRFPEQSSDGGGSVQSGNDPESLTPEGHAAIA
                     EYEFAGTGFYVGNGFIVTNRHIVQPWIADDRAQSVGSNFHAQPRLKKLIAYFPDYTQQ
                     IPLKFIAAAQKDDLAVCSLDVSEVPESIPLLPLESEPDAVAVGKTVVMMGYPSGPDRL
                     LALLDEAESRSIQQRFGSPIAILGYLAETKRVQPLTTQGNITDLDVRRIVYDARTAEG
                     GSGAPLFGPSGRVIGVNFAVFTENQASNFAVPIRFAVALLERAGWEPPAGKDGKDLDT
                     TSQPATHPTTPR"
     gene            1182..3185
                     /locus_tag="VFH91_07425"
     CDS             1182..3185
                     /locus_tag="VFH91_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862861.1"
                     /translation="MTSPRHRVSFLVVVLTLMVSPVRIFAQETGAQSKDNDLSQILRL
                     ERIEIPNGAELITIHAKLDGLDQTTADKWVPLVSILRDNLGDNSYENDRLRYVWPLTY
                     TRPSFKQRLAAAVPFFYSRVGNKEHVSSKPPPPAMDLAAPESQVWDKIFWTALQNILL
                     DPYGTPIKASTSTYRRNINDYRRAHIVRALSVLTLYQAVKGESAFSDSEMAEMQARLL
                     LTDKTFGGLVDDSNLQRYYTKKVAEVRDERGHNWELLRQRAEAESLFFEPLLMPDGSP
                     THAILWVAKSDLEKRQGASYSGRFLNIANPWTDKRLLQWQGFVETRYFNAEHMPVDQD
                     TPGAKAVEMIPLGLYGLDNPKIPMLLVDFRDSYNPKKREMSRRVLNDVTRNILSVSKF
                     GNLPYFLGRTVFDFVTGRRGIDVNQPSRLQTYSQLKLLLALNNSMDPDLRQEVNGRLE
                     HVSLNPFENDLEAEAKLARGQYAALIAFARDPEGLVARVDRDRRAEMTALDHGRKAQV
                     AFNVLHVLSFGRYIHREEASPELEARLDISRRLDFHSRFLAQIAKSSVDIDVSWNLND
                     VRRSLAFISEHGTEAGSTAVMAAAKIFSRTNDTETRRACLDSLSRISNPKARDQLLRI
                     SQNTSLDQSWRDLASQYLAKVNEPLQPIVTTASDLSHKSGQPR"
     gene            3182..>3918
                     /locus_tag="VFH91_07430"
     CDS             3182..>3918
                     /locus_tag="VFH91_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862862.1"
                     /translation="MRSGTQLRYHGGLRKRSVLAVCLLLLFCAGSSAQTKRLVVIKCD
                     GLPYDFVDRLVKQRDPASGKSQLPWIDHIFYQQGTRLSSFYVRGMSLSAPSWSLLETG
                     QHLQIKGNVEFDRYTLHSYDYLNFFPLYVAGALGTRIDMPAVEVLDSLGVPLITDAYA
                     HDERYMTFSLFLRGPRYSTLQESLQNRFRKSPKELFDEWTMGFELRNALTDQLVREML
                     QKLNNPKLKYLDLYLADFDHVAHHNNDL"
BASE COUNT          998 a         1004 c          969 g          947 t
ORIGIN      
        1 ttcgttctat ttcgtcgagg ggggcaccgg acgaccatta cgattccctg aacagagtag
       61 cgatggaggc ggaagtgttc aaagtggcaa tgatccagag tcacttacgc ctgaaggcca
      121 cgccgctata gctgaatatg agtttgccgg caccgggttt tatgtcggca atggttttat
      181 tgtgacaaac cgccacattg ttcaaccgtg gattgcagat gatcgagcgc agagcgtcgg
      241 cagtaacttt cacgctcagc cgcgactgaa aaaactgatc gcttatttcc cagattacac
      301 acagcaaatc ccgttgaagt ttattgcggc ggctcaaaag gacgatctcg cggtatgttc
      361 tctggacgtc tcagaggttc cagaaagtat tccgctgtta cctttggaaa gtgaaccaga
      421 cgctgtggca gtcggtaaga ctgttgtgat gatgggttat ccatcaggtc cagatcggct
      481 cctcgcgcta cttgacgagg ctgagtcacg aagtattcaa caacgattcg gatctcccat
      541 tgctattctc ggatatcttg cagagacgaa gcgagtacaa ccgttaacca cacagggtaa
      601 tattacggac ctcgatgtgc gcaggatcgt ttacgacgcc cgcacagccg aaggtggatc
      661 tggcgctcca ctctttggac catcaggtcg agtaatagga gtaaactttg ccgtttttac
      721 agagaatcag gcctcaaact tcgccgttcc cattcgcttc gcggtagcac tccttgagcg
      781 tgcaggctgg gaacctccgg ctggaaagga tggcaaggac ctcgacacca ccagtcaacc
      841 tgccactcat ccgacaactc ccagataaaa ccccagtaac tttaacaggt gtcaagataa
      901 cgaaagttag tgtacaaacg tccagttagt acgtaaggcg aaccgatcaa tggcactgag
      961 gcatcagagg gccactgtta cacgcaagaa tctgtcatag acgaaacaga aaattggtta
     1021 gcgatggtcc acactgatgt atatttatcg aacggtacga ggggcacaat acttgctctt
     1081 cgaatgccaa ttgattgaaa agtgcccgtg ttctacaggg aagggtgcag gcaagatttt
     1141 ccaactgaca aaatttgttg cgtactactg acgaaactag catgacgtcg ccacggcatc
     1201 gagtaagctt cctggtagtt gttcttaccc tcatggtctc gcctgttcgt atctttgcac
     1261 aagagacagg ggcgcagtct aaagacaacg atctatccca gatattgcgt ttagagcgaa
     1321 ttgagattcc caatggcgct gagctgatta ctattcacgc gaaacttgat gggttggacc
     1381 aaacgaccgc tgataagtgg gttcccctcg tgtcgatact ccgcgataac ctgggagaca
     1441 acagttacga gaatgatcgg ctcagatatg tctggccgtt aacttacacg cggccgagct
     1501 tcaaacaacg actagccgct gcagttccct tcttttattc gcgcgtcgga aacaaagaac
     1561 acgtttccag caaaccgcct ccgccagcaa tggacctggc tgcgccggaa agccaggtgt
     1621 gggataagat tttctggaca gcgctacaga atatcttgct cgatccatac ggtacgccga
     1681 tcaaagcatc aacaagcact tatcgtcgaa atatcaatga ctatcgaagg gctcacatag
     1741 ttcgcgctct ttcagttttg accctgtacc aggcggtcaa aggcgagtct gctttttcag
     1801 attctgaaat ggctgaaatg caagctcgac tcttgcttac cgacaaaaca ttcggtggcc
     1861 tggttgacga ttcgaatctt caacgttact acacgaaaaa agtcgccgaa gtgcgcgatg
     1921 aacgcggtca caactgggaa ctcctgcgcc agcgagctga agctgaatcg cttttcttcg
     1981 aaccgttgtt gatgcctgac ggcagtccaa cgcacgcgat cttgtgggtt gctaaatctg
     2041 acctggagaa acgtcagggt gccagctaca gcggacgctt tttgaacata gcaaatccgt
     2101 ggaccgacaa gcggcttttg cagtggcaag gctttgttga aactcgctac ttcaacgctg
     2161 aacatatgcc ggtggatcag gacacgccag gagctaaagc agttgagatg attccgcttg
     2221 gactctacgg cctggacaac ccgaagattc caatgttgtt agtcgatttt cgcgacagct
     2281 ataaccccaa aaagcgtgaa atgtcgcggc gcgtgctcaa cgacgttact cgaaacatct
     2341 tatcggtatc gaagtttgga aaccttccct actttctcgg caggaccgta ttcgattttg
     2401 ttacgggacg ccgaggaatc gacgtcaatc aaccctctcg tttacagacg tactctcaac
     2461 ttaagttgtt gctcgctctg aataactcga tggacccgga tctacgacaa gaagtcaacg
     2521 gccggctcga acacgtatcg ctcaatccat tcgagaatga ccttgaggcg gaagccaagt
     2581 tggcgcgggg tcaatatgcg gcgttaatag ctttcgctag agatcccgaa gggcttgttg
     2641 ccagggtgga ccgtgaccgc cgggcagaaa tgacagcttt ggaccatggg cgaaaggccc
     2701 aggtcgcatt caacgttttg cacgtgctga gcttcggcag atatatccat cgcgaagagg
     2761 cgtcgccaga gttggaagcg cggctggata tctcccggag gcttgatttc cactccaggt
     2821 ttctcgccca gattgccaag tcgagtgtgg acatcgacgt ctcgtggaat ttgaacgatg
     2881 tacgtcgatc gttggccttc atttcagagc acggaaccga agcgggtagc acggctgtca
     2941 tggctgctgc aaagatcttt tctcgtacga acgataccga aacaagaaga gcatgccttg
     3001 acagcttatc cagaattagt aacccgaaag cacgcgatca actattgcgc atctctcaga
     3061 atacttccct ggaccaatcg tggcgtgacc tggcatcaca atacctcgcg aaggtcaatg
     3121 aaccccttca gcctattgtc accacagcct cggacctttc gcataaatcc ggccagccga
     3181 gatgaggagc ggtactcaac tcagatatca cggtggtctg cggaaaaggt cggttctcgc
     3241 ggtctgcttg cttctgcttt tttgcgcggg ttcctctgct caaactaaac gactcgtagt
     3301 cataaaatgc gatggccttc cttatgattt cgtagaccga ctcgtaaagc aacgagatcc
     3361 tgcttcgggt aaaagccaac taccctggat cgatcacatc ttctatcaac aaggaacaag
     3421 attatcgagc ttctacgtaa gagggatgag cctttctgca ccctcctggt cgctgctgga
     3481 aacgggtcaa catttacaaa tcaaaggcaa cgttgaattc gaccgctata ccctccactc
     3541 ttacgattac ttaaatttct ttccgttgta tgtcgctgga gctctcggta ctcgcatcga
     3601 tatgcccgca gtcgaggtac tcgattcact tggtgtaccg ttgataaccg atgcatatgc
     3661 tcatgacgag cgttacatga ccttttcgct attcttacgt ggtccacgat attcaactct
     3721 tcaagagagt ctgcaaaatc gcttcaggaa atcccccaag gaattgtttg acgagtggac
     3781 catgggattc gagcttcgca atgccctgac cgatcaactc gtcagagaaa tgcttcagaa
     3841 gctcaacaat ccgaagctga agtacctgga tttgtacctt gccgattttg atcacgtagc
     3901 gcatcacaat aacgatct
//
LOCUS       DASSFP010000335         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1580542, whole genome shotgun sequence.
ACCESSION   DASSFP010000335 DASSFP010000000
VERSION     DASSFP010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580542"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFH91_07435"
     CDS             <1..554
                     /locus_tag="VFH91_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6862863.1"
                     /translation="LTVFRMVLIPVFVSLLFYQRFVLALIVFIVAGITDGLDGLLARR
                     FNQRSQLGTILDPIADKLMLVTSFVVLSMKAVFPQPLPPHLPVPFWVTVAVISRDVFI
                     LVGAAAINIVTGFRGFRPSWLGKLNTTVQIVAIALIMVAASIPYGTGYYLPTVYTTVF
                     ALAVLSGGHYVFFVSKLLNEDRR"
     gene            879..2795
                     /gene="dnaK"
                     /locus_tag="VFH91_07440"
     CDS             879..2795
                     /gene="dnaK"
                     /locus_tag="VFH91_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6862864.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPTVITNSEGGRTTPSVVAFTK
                     DGSRLVGQVAKRQAVTNPENTVYSIKRFMGRRYNEVSEEIKQVPYKVQGAGSGDVRVL
                     AGGKEWSPPEISAIILQKMKQSAEDYLGQKVDKAVITVPAYFNDSQRQATKDAGKIAG
                     LEVMRIVNEPTAAALAYGLDKKKDETIAVFDFGGGTFDVSILEVGEGVVEVKSTNGDT
                     HLGGDDIDERLIEWIIDEFKKDQGIDLSNDKMALQRLKEAAEKAKIELSSTMETEVNL
                     PFVTADQSGPKHLAMKLTRARFEQLVDPLLTRLRSPVEQALKDAGIDAKKIDEVVLVG
                     GSTRIPKVQEIVKEMFGKEPNKSVNPDEVVAIGAAVQAGVLSGEKTDILLLDVTPLSL
                     GIETLGGVFTKLIERNTTIPTRKSEIFSTASDNQTSVEVHVLQGERPMASDNRTLGKF
                     HLVGIPPAPRGMPQVEVTFDLDANGIVNVSAKDMGTGREQKITITASSGLSKDEIDRM
                     MKDAESHSADDARKRDEIEARNRLDGLVYQMEKTLSDNREKVGAVAGEVETAIADAKK
                     ALAEGGVEKLNDAFNNLQTVSHKLAEALYQQAPPPGEPASGDQASAAGASSSGGSSDD
                     NVIDAEYVDVDENK"
     gene            2909..>3528
                     /locus_tag="VFH91_07445"
     CDS             2909..>3528
                     /locus_tag="VFH91_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET6862865.1"
                     /translation="MAKQDYYEILGIKRDAKPDEIKKAYRRLARKYHPDVNPGDKAAE
                     ERFKLTSEAHDVLSDPKKRSVYDKFGQYSDNLADAAARGAGPSSGSRTSPGFDFTGFD
                     WGNASSGTSGGGGTSFRDIFADLFGGGTAKDKEPPRPQPQRGIDIEMPLALSFEEAIK
                     GLTTNITVNRSEQCSRCNGAGDIGGPVVVCSTCKGTGQVQRAGGRLR"
BASE COUNT          900 a          820 c          970 g          838 t
ORIGIN      
        1 tgcttaccgt tttcagaatg gtgctcatcc ctgtgttcgt gtcgctgctg ttctatcagc
       61 ggtttgtgct tgccttaatc gtctttatag tggctggcat taccgacgga ctggatggtt
      121 tattagcccg ccgttttaac caaaggtctc agctcggcac gatactcgat ccaattgcag
      181 acaaattgat gcttgttact tcgtttgtgg tgttgtcgat gaaggcggtt tttcctcagc
      241 cgttgccgcc acacttacca gtcccatttt gggtcactgt ggcagttatt agccgtgatg
      301 tgttcatttt agtcggagcg gctgcgatca atatcgtcac gggcttccgg ggattccgtc
      361 catcctggtt gggcaaattg aatacgacag tacagatcgt cgccatcgcc ctgattatgg
      421 ttgcagcgag cattccctac gggaccggct actacctccc tacggtttac acaactgttt
      481 tcgctctcgc ggtactgtcg ggtggccatt acgtcttctt cgtctcaaaa ctgctgaacg
      541 aggatcggcg ttgaatccgc tgtagaaagc acctaaacaa tccaatgatg tcgcttttgt
      601 tgcaaatcca atagcgaatc gaatggactt cctttgcgcc agtcggaagc ccgaaacccg
      661 gaggaaaaca ctccagctac caaccattct gaaggcttgc tacaacctta atcaaaaagt
      721 tgacaacaac acactcaggt attatattgg cgaggcgttc aactaagggt gtcgttgaga
      781 atagggagga gccttctgag gccagcggcg tgaggccctt attagtctga atagctggtc
      841 tgaatgtctt acccgagaag ggctttggag attaaaagat gagtaagatt attggaattg
      901 acttgggaac caccaactcg gtagtcgccg ttatggaagg aggcgaacct accgttatca
      961 caaactccga aggtggccgc accactccgt cggtcgttgc ttttacaaag gatggaagcc
     1021 ggcttgtggg acaggtggcg aaacgccagg cggttacgaa tccggagaat accgtttatt
     1081 cgattaagcg atttatggga cgtcgctata acgaagttag cgaagaaatt aagcaagtcc
     1141 cgtacaaggt tcaaggggcg ggaagtggcg acgttcgcgt gcttgccgga ggtaaggaat
     1201 ggagtccgcc agaaatttcc gcaattattc ttcagaagat gaaacagtcg gctgaagatt
     1261 atctgggaca gaaggtcgac aaggcggtca ttaccgtccc agcatatttc aacgactctc
     1321 aacgccaggc gactaaagac gccggaaaaa ttgccggcct cgaagtgatg cgcatagtta
     1381 acgaaccaac cgcagctgcg ctggcatatg gtcttgataa gaaaaaggac gagacgattg
     1441 cagtattcga ctttggtgga ggtactttcg acgtctcgat tcttgaagtt ggtgaaggcg
     1501 ttgtcgaagt caaatcgacc aatggtgata cccacctggg tggtgacgat atcgacgagc
     1561 gcttgatcga atggatcatc gacgagttca agaaggatca aggtattgat ctttccaacg
     1621 ataagatggc cctccaacga ttgaaagagg ctgctgagaa ggcaaagatt gaactctcca
     1681 gcacgatgga aacagaagtg aatcttccct tcgtgactgc agaccagtca ggtcctaagc
     1741 atctggcgat gaaactgact cgagccaggt ttgagcagct ggttgatcca cttttgaccc
     1801 gattgaggtc gccggttgag caagctctaa aggatgccgg catcgatgca aagaagatcg
     1861 atgaagtggt gcttgtaggt ggttcaactc gaatcccgaa ggttcaggag atagtcaaag
     1921 aaatgttcgg caaagagccg aacaagagtg taaacccgga tgaagttgtg gctattggtg
     1981 ctgctgttca ggctggtgtt ctgtctggtg aaaagactga cattctgctg ctcgacgtga
     2041 cgccactaag ccttggtatt gaaacactcg gaggtgtatt cacgaagtta atcgaacgaa
     2101 acaccacgat tccgacgaga aagtctgaaa ttttctcgac ggcttcagat aaccaaactt
     2161 cggtcgaggt tcatgtactt cagggtgagc gaccgatggc gagtgacaat cgcacgctcg
     2221 gtaaattcca tctcgtagga attccaccag caccgcgagg tatgccgcaa gttgaggtca
     2281 cgttcgatct tgatgcgaat ggtattgtca acgtgtctgc aaaggacatg ggaacaggcc
     2341 gtgagcagaa gatcaccatt accgcttctt ctggattgtc caaggatgaa atcgatcgga
     2401 tgatgaaaga tgccgagtcg cattcagccg acgacgcacg taagcgggat gagatcgaag
     2461 cacgcaatcg actcgatggc cttgtctatc aaatggagaa gacgctgagt gataaccgtg
     2521 aaaaggtcgg agcggtagcc ggcgaagttg aaacggcgat tgccgatgct aaaaaggctt
     2581 tagccgaagg cggcgttgaa aaactcaacg atgcgttcaa taacctgcaa actgtttcgc
     2641 ataaactagc tgaggctctc tatcaacagg cgccacctcc aggtgaacca gcgtccggtg
     2701 atcaagcttc ggcggctgga gcttcttcgt cgggcggttc aagcgacgat aatgtgatcg
     2761 acgctgaata cgtcgacgtt gacgaaaaca aatagatttt caattcgtat cttccgttgc
     2821 cgattggttg gtgcctgaat cgagccctga gttatagctg gattaaggct ccgatcagtc
     2881 ggcaattggc aattggaaat cgaaaataat ggcaaagcaa gattactacg aaattctcgg
     2941 catcaagcga gacgccaagc ccgatgaaat aaagaaggcc tatcgacgtc tggcccggaa
     3001 atatcacccc gatgtcaatc caggtgacaa agcggctgag gaacgcttca agttgacatc
     3061 agaagcgcat gatgtgctgt ctgatccaaa gaagcgcagc gtctacgata agtttggtca
     3121 gtactctgac aatcttgcgg acgccgccgc tcgcggtgct ggtccatcga gtggcagtcg
     3181 aacgtcaccg ggttttgact ttaccgggtt cgactggggt aatgcgtctt cgggcaccag
     3241 cggcggtggc gggacgagct ttcgcgacat attcgccgat ttgtttggag gcggcacggc
     3301 aaaagataaa gagcctccgc ggccacagcc tcaacgcggc atcgacatcg aaatgcccct
     3361 ggcgctttct ttcgaagagg caattaaggg tcttacaaca aacatcactg taaatcgttc
     3421 tgagcagtgt tcacgttgca atggtgccgg agacattggt gggccggttg tcgtttgctc
     3481 tacgtgcaaa ggcacaggtc aggtccagcg agctggtggt cgtctacg
//
LOCUS       DASSFP010000336         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2033474, whole genome shotgun sequence.
ACCESSION   DASSFP010000336 DASSFP010000000
VERSION     DASSFP010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033474"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFH91_07450"
     CDS             <1..1066
                     /locus_tag="VFH91_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6862866.1"
                     /translation="LLELAVLPLFVIGLIVLPVRAQSADGANSVEISKVIKAFTAKES
                     EFRTALNNYSFKRDALLQSIGMGGQVIGEYHKVSLLTFDDSGNRYEKISFFPMPSLAE
                     ITKEDIEDLGGVEPFALEPGNISKYDIRYVGKEKIDDLNLYVFDVAPKVMPSAKNLKD
                     RLFLGRIWVDDQDLQIVKTKGKGVPETKNNKFPVVETYREHIDGRYWFPTYSYADEEL
                     IYENGGSLHIRMKVRYSDFTPARATLKVTEVGEDENPSSKEVTKPVEEGVLNPKATGL
                     PIPRYPEEARRLKIAGRVTVRVVVDENGKVISATATDGPAALRQSAEDAARAATFTPK
                     VVGGITVKMTGVLTFDFKLP"
     gene            complement(1068..1847)
                     /locus_tag="VFH91_07455"
     CDS             complement(1068..1847)
                     /locus_tag="VFH91_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6862867.1"
                     /translation="MEDSAKAGTTPDESNTNERRNFLRRLHYWWSLFVAGALLGVLGP
                     PILIVAWLVNKHDLVYPVALFGARAWLKLSGVRVVVKGLELLDPKQSYVFVSNHRSYL
                     DTATLFVYTGRRIGLLAKKELLKIPVLGVGMGFVNVMAIDRSNRESAIRTTEAAARRI
                     QSGVSFAVFVEGTRARPGELLPFKKGAFYMAKQAGVPIVPVAIKYSDDLMGKGTGEAR
                     SGTMEMILLKPVRTDTVQSDEDMTRFIHEVRHSIAHELSSP"
     gene            1868..4369
                     /locus_tag="VFH91_07460"
     CDS             1868..4369
                     /locus_tag="VFH91_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET6862868.1"
                     /translation="MTRHRLAIGSLAVLLYLAALPNSLAAQQAKLQPAPIGAVPSPRS
                     ILGFTPGDDRTIADWEQITNYFSLLDKRSNRVLVQTLGESTLKRPLIVAFISARENIL
                     ALEKYKDIQQRLADPRKVTSNIERDKLVRDGKVVVAISCSIHSTEIVASQMAMQLAYE
                     LAVAEDTQTKEILQNTILILIPSPNPDGIDIVSNWYRKTLGTPFEGKEPPELYHHYAG
                     HDDNRDWFMLNLKETRLITRLFWHEWFPQVVYDVHQQGSNGSRFFIPPFYDPANPNIS
                     PTLLRQVGLLGHKMAADLQSQGYKGVLTNALYDTWWHGGFRTAPYFHNSIGILSEAAS
                     ARLMTPASVTADQLARSSTRGMRSASEASTNFPDPWAGGTWRPKDIMSMELIAARSLL
                     SLTAKYREEYLANFYELGKESIERASKPDEPIAYLIPAGQGRDEAVAKMLGSLIEQGV
                     EVFRLDQELHVTFGPQSLQRTNPANEKLGTYRTVIRSAGVMQEVPAGSYIIFLAQPQR
                     SNVEALFEPQIYPNRLTGQGEAERPYDVAGWTLPLQMGVDAPAITAIREKAAEQKLML
                     LHSGDVVREDLALPPVKGERSPIKNPVSVPVRIGIYKGNTGNMDEGWTRFVFDTFNVP
                     YATIKDAQVRKGNLNSNYDVVILPSQRASEIVQGNASGSLPADYTGGITEAGVNHLKE
                     FVSNGGTLICFDASCNLPITQFKLPLRNTLEGLRSSEFYSPGSIVSLEVDSSAPSFQG
                     LPHKLPAYFINSSAFASTDPSVKVIARYAKEDVLLSGWLLGEDKLRGQIALAETNIGK
                     GRVVLFAFRPQHRGQTWATLPLIWKAIASSQDQSE"
     gene            4596..>5017
                     /locus_tag="VFH91_07465"
     CDS             4596..>5017
                     /locus_tag="VFH91_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862869.1"
                     /translation="MKMRALLLLLPLLLISVSIDSLAQVRPRRVGDQTPVPQTQSPSK
                     PPVLGGSSSPGTRQPSKQTTEGNPANAPEEVDAGDIVKVDTTLVTIPVSVMDRDGRYV
                     PNLQKEDFRIWEDGVEQQVAFFASVDKPFSVVLMLDTSP"
BASE COUNT         1306 a         1263 c         1253 g         1195 t
ORIGIN      
        1 acttctggaa ctggcggtgc tgccgctatt tgtaatcggc ctgattgtgt tacctgttcg
       61 agcccaatcg gctgacggcg caaattcagt ggaaatatcg aaggtaatca aagccttcac
      121 ggccaaggag agcgagttcc ggactgccct gaataattac tcgttcaaac gagatgcgct
      181 cctccagagc attgggatgg gtggtcaggt cataggtgag taccataagg tttcgcttct
      241 cacatttgat gattcgggga atcgatatga gaagatcagc tttttcccga tgccatccct
      301 ggctgaaata acgaaggaag atatcgagga tctgggcggg gtggagccgt tcgctttgga
      361 gcccggtaat attagcaagt acgacattcg ctacgttggc aaggaaaaga tagacgacct
      421 taacctatac gtgttcgacg tagcgcccaa ggtgatgccc agtgcaaaga accttaagga
      481 tcgtctgttt ttgggtcgta tctgggttga tgatcaggac ttacagatcg taaaaacaaa
      541 aggtaaaggc gttccagaaa caaagaacaa caagtttcca gtcgtcgaaa cctatcgtga
      601 acatattgat ggccgctact ggtttccgac ctattcatat gctgatgaag agctgatcta
      661 tgagaacggt ggttcacttc atatccgcat gaaagttcgc tactcagact tcactccagc
      721 acgtgcgacc ttgaaggtaa ccgaggttgg tgaagacgag aatccttctt ctaaagaagt
      781 caccaaaccg gtcgaagagg gagtgttaaa tccgaaggca acgggattac ccattccccg
      841 ttatccggaa gaagcgagac gattgaagat tgcgggacga gttacggtca gagtagtcgt
      901 cgatgagaac ggcaaggtaa tctcagcaac cgctactgac gggccggcgg ccttacgaca
      961 atcggccgaa gatgctgcgc gtgctgcaac gtttacacct aaagttgtcg gcgggattac
     1021 agttaaaatg accggggtcc tgacgtttga tttcaaactt ccgtaattca gggcgagctt
     1081 aattcgtggg caattgagtg tcgaacttcg tggataaagc gggtcatgtc ttcgtcactc
     1141 tgaaccgtat ctgttctgac tggcttcagc aggatcatct ccatcgttcc tgatcttgct
     1201 tcgccagtgc ctttccccat taagtcgtct gagtacttga tggcgaccgg aacgatcgga
     1261 acacccgctt gcttcgccat gtaaaaggcc cccttcttga acggcagtag ctctcccggt
     1321 ctcgcacgcg ttccttccac aaacacagcg aacgagacac ccgactgaat ccgtctcgcg
     1381 gccgcttccg tagtgcgaat agcactctct ctattagacc gatcgatcgc catcacgttc
     1441 acaaaaccca ttccgacacc aagtactggt attttcagta actccttctt tgcaagtaat
     1501 ccaatacgtc tgccggtata gacaaagagt gtcgccgtat cgagataaga gcggtgatta
     1561 gagacgaaga catatgattg ttttgggtcc agtaattcca atcccttcac gaccactctg
     1621 acgccactca acttcaacca ggctcgggcg ccgaataaag ccaccggata gaccaggtcg
     1681 tgcttattta ctaaccacgc aacgatcaga atgggtggac caagcacgcc aagaagtgcg
     1741 cccgcaacga acaacgacca ccagtagtga agtcgccgaa ggaagtttcg ccgttcgttt
     1801 gtgttagact cgtcgggagt tgttccggct ttagcgctat cttccataaa tcgcaaaagg
     1861 aattctaatg actaggcatc gactggcaat cggaagtctg gccgtgcttc tgtacctggc
     1921 cgcgcttcca aactcactgg cggctcagca agcaaaatta caacctgcac caattggggc
     1981 agttccttct cccaggagta ttctgggctt tacaccagga gacgaccgca ctatcgccga
     2041 ttgggaacag ataacaaact acttttccct tctcgataag agatcaaacc gagttctagt
     2101 tcagacatta ggagaatcga cgctcaaacg gccactgatc gtcgcattca tcagtgcgcg
     2161 agagaatata ctggcactcg aaaaatacaa agacattcag caacgattag ctgaccctcg
     2221 gaaagtcacg agcaacatcg aacgcgataa attggttaga gatggaaaag ttgttgtcgc
     2281 catatcatgc tcaattcatt ctaccgagat tgtcgcgtcg caaatggcga tgcagctcgc
     2341 ctatgaacta gctgtggccg aagacacaca gacgaaagag attcttcaaa atacgatctt
     2401 gatattgatc ccgtctccga atccagatgg gattgacatc gtctcaaact ggtatcgcaa
     2461 gacactcggg actcccttcg aaggcaaaga acctccagaa ctctatcacc attacgccgg
     2521 ccacgatgat aatcgcgact ggttcatgct caacctgaaa gagacgcgtt tgatcacccg
     2581 attattctgg cacgagtggt tcccacaggt tgtctacgac gtacatcaac aaggttcgaa
     2641 tggttcccga ttcttcatcc ctccttttta cgatccagcc aatccgaata tatcgccgac
     2701 cctcctgaga caggttggat tattgggcca caaaatggcg gcggacctgc aaagtcaggg
     2761 gtacaaaggc gtgcttacaa acgctcttta cgacacctgg tggcatggag gctttaggac
     2821 cgcgccgtac ttccataact caattggcat cttgtcggaa gcagcgagcg cgagactcat
     2881 gacgcccgca agcgtcacag ccgaccaact agccaggtca agcactagag gaatgagaag
     2941 cgcttcagag gccagtacca actttcctga tccgtgggcc ggaggaacgt ggcgtcctaa
     3001 agacattatg tctatggaac tgattgctgc cagatctttg ctgtcgctga ccgcgaaata
     3061 ccgagaagag tacctggcta atttttatga gctcggaaaa gagagtattg agcgagcatc
     3121 caaacctgac gagccaattg cttacttgat tcctgccgga caaggtcgcg atgaagcggt
     3181 agcgaaaatg ctcgggagtc tgatcgagca gggagtggaa gtattccggc ttgatcagga
     3241 actgcacgta acatttggac cacagtcgtt gcaaagaaca aatccagcga atgaaaagct
     3301 cggcacatat cgcacagtca tccgatcagc tggagtgatg caagaagtac ctgcaggaag
     3361 ctacataata tttcttgctc agccacaacg ctcaaatgtt gaggctttat ttgaaccgca
     3421 aatctatcca aaccgtttga cagggcaggg tgaagccgaa cgtccgtacg atgttgccgg
     3481 ttggactctt cccttgcaga tgggagtaga cgctcctgcg attaccgcca tccgtgaaaa
     3541 ggctgccgaa caaaagttaa tgttgttaca cagtggagat gtcgttcgag aagatttagc
     3601 cttgcccccc gtgaaaggcg aacggtcgcc aatcaaaaac ccggtttcgg ttccggttcg
     3661 cataggtatc tacaagggca atactgggaa catggacgaa ggatggacca gatttgtatt
     3721 cgacacgttc aatgttcctt acgcaacgat taaggacgcg caagttcgca aggggaattt
     3781 gaactcgaac tatgatgttg tgattttacc atcgcaacgt gcatcggaga tcgtgcaggg
     3841 aaatgcttcg ggtagtttgc cagcagacta caccggagga ataacggagg ccggagtaaa
     3901 tcacttgaaa gagttcgtca gcaacggagg cacgctgatt tgcttcgatg cgtcatgcaa
     3961 ccttccaata acgcagttta agctgccttt gagaaataca cttgagggtt tacgaagttc
     4021 tgagttctac agtcctggct caattgtttc actcgaggtc gatagttcag cgccttcatt
     4081 tcagggacta ccgcacaaat tgccggccta tttcataaat agttccgcct ttgcctcaac
     4141 cgacccaagt gtcaaggtga tcgcacgtta tgcaaaagaa gatgttctgc tgtccgggtg
     4201 gctgttgggc gaagacaagc tgcgcggcca aattgccctg gccgagacca atattggtaa
     4261 aggcagagta gtgttgtttg ccttccggcc gcagcatcgg ggacagacct gggctactct
     4321 acctctgatc tggaaggcta ttgcgtcgag tcaagaccaa tctgaatgaa acaaaaaact
     4381 gtcaattttc cgaggaaata tcttcggaat ggaggattct tgcactcccg ggcgcttaaa
     4441 ctcttgtgcc tcgcctccac gaaggttatc tttgtcccac ggtttttctc ttcccagatt
     4501 cccccgttca aaaaccgcaa gcatctattt tgggttgagt gcatcaaagt gaggcttttg
     4561 ggcctctcct tggtctgtac gatcggagcc tccgtatgaa aatgcgcgca ctgttactgc
     4621 ttctcccttt gctgttaatc agtgtttcaa ttgattcgtt ggcccaggtt cggccgcgca
     4681 gagttggcga tcaaacccct gttcctcaaa cgcagtcgcc gagtaaacca ccagtccttg
     4741 gcggatcgag ctctccggga actcgtcagc cttcaaagca aactactgaa ggtaatcccg
     4801 ctaacgcgcc cgaggaggtc gacgccggtg acatcgttaa agttgacacc accctcgtaa
     4861 ccattccggt tagcgtaatg gaccgggatg gacgctatgt ccccaactta caaaaagaag
     4921 actttcggat ctgggaagat ggagttgaac agcaagtcgc tttctttgcc tcagttgata
     4981 aaccgttctc tgtcgttctg atgctggata caagtcc
//
LOCUS       DASSFP010000337         5458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_89131, whole genome shotgun sequence.
ACCESSION   DASSFP010000337 DASSFP010000000
VERSION     DASSFP010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89131"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFH91_07470"
     CDS             <1..584
                     /locus_tag="VFH91_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862870.1"
                     /translation="VHQDLTSILGFCKSHRGEYEAPPNAQALWLRIRNVIEADGGRRA
                     ATIEPRKSFLGGLMGRSWQLSLPQLVSSVVAIVVLVSLATVVGIRRWGMAPAAMQTMQ
                     IEPTNPGSSVNDRVWQRQQVISYWNQRIEVNKTRWSPEMRETFDRNMAVIDQAVSESM
                     NELNRNPHDDIAEQMLNESLNDKLTLLKEFSDL"
     gene            644..1402
                     /locus_tag="VFH91_07475"
     CDS             644..1402
                     /locus_tag="VFH91_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET6862871.1"
                     /translation="MNAKFSTWSVSSLAKLISIFVIGSLLGTVAAQSPQRLSKRYTVG
                     KNVRVELKNVSGTIEVESWNRDEIRLSATIESPSAHVVPKQIDQSLVIDVMSDNKARG
                     DVGDVNFKLQVPISSSVDLETRRGQISVSNLRGASVRAHVSSEGDITLSNIAATQVIA
                     QNTIGNIFFDGEFSRGGTYEFKSTKGDITIRIPGNSAFRMVATSPARKIKLGDFWNDG
                     FKTQDGRKYVGDVGDGRSSVSVTNFNGSITFMRR"
     gene            1540..2418
                     /locus_tag="VFH91_07480"
     CDS             1540..2418
                     /locus_tag="VFH91_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862872.1"
                     /translation="MRHASKLLLAAITSLLFSIPAASQTTSQKDKSQVLPTAPLLTRT
                     ATRRESQRLGYGGTLTLVGAPRGSVTIEGWSKSEVELTADIELKATSEEDLNRLASIN
                     TFVFDQDLNHVSVLTTGTHDRVYMKKFKNFPKALLNLPWKIDYRIRVPATTDLEVNAG
                     HGAVKISGVEGAMRISATESDSSFTLTGGVVSATIAVGTVTLSIPTRSWRGGGADIRV
                     ASGALNVEIPPGFSGDIDADVLRIGQIVNSYDGLEVREKPGITERTIRARAGAGGPFF
                     KFTVGDGILAIKKTPS"
     gene            2517..3080
                     /gene="dcd"
                     /locus_tag="VFH91_07485"
     CDS             2517..3080
                     /gene="dcd"
                     /locus_tag="VFH91_07485"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET6862873.1"
                     /translation="MIKSDKWIRRMAEEHGMIDPFEPGQVKEIDGRRIVSYGTSSYGY
                     DIRSADEFKLFTNINSTIVDPKEFDSKNFVDVKSDSVIIPPNSFALARTVEYFRIPRN
                     VLTICLGKSTYARCGILVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGVAQVVFFE
                     SDEVCETSYKDRGGKYQGQRGVTLPKL"
     gene            3090..3539
                     /gene="dut"
                     /locus_tag="VFH91_07490"
     CDS             3090..3539
                     /gene="dut"
                     /locus_tag="VFH91_07490"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET6862874.1"
                     /translation="MSIPQRSVSTGELHFKKLDSRATLPVRGSVASAGLDLFCIEDLR
                     LEPKARILARTGIAVAIPVGYYGRVAPRSGLAVKNGIDVLAGVIDSDYRGELCCALLN
                     TGDEAVVLPQGSRICQLIIERIITPEPIWVEDLDNTSRGDGGFGSTG"
     gene            3580..5238
                     /locus_tag="VFH91_07495"
     CDS             3580..5238
                     /locus_tag="VFH91_07495"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6862875.1"
                     /translation="MITHRNSLKKCALILSFVVLTGTVLGQSLPVAPTTSLSGSEREL
                     SGKVRTDTIRAIVTALSADDMQGRGTAQPGGDKAAQFLADKFAKLGLKPLGDKGSYLQ
                     SINFREIQFLSETSLKVGTENLKLGQDFFITPPYSGDKNVKGDLVFVAYGVSTPFLKR
                     NDLQGIDLRGKVAIVLEGPPPEVNKDSWKQAHAQSMVLRGLIARGAAGIILLNNGPQE
                     HTFAELADYLTRRQIELADEAEFPDFLPPFLNVSDATAEKLFQASGITKEQALAKAAG
                     ENFAPINLQQSASITVRLKKSRGTSSNVVGVLAGTDPLLKTEALVYSAHYDAYGLSAD
                     NKVYHGAADNALGVGQMIAIAEVLSKAKLRRSVIFMALTGEEYGGYGSTYWVKNPTWK
                     IKQVAADLNMDGMGTEVYGPVKVVVGYGAEHSSLGSTLNEVAAASGLKVIPDPMPDEK
                     SFYRSDHYFFVKRGIPGIMLLGAPEGDSSVWIDRLKKWEKADYHQPTDVIRPDWHWDG
                     PSTIAKVALVMGLRIANADAMPSWLPSSIFNRERGTDKPAPPEP"
BASE COUNT         1377 a         1285 c         1394 g         1402 t
ORIGIN      
        1 gtgtgcatca ggatctgacg tcgatcctcg gcttttgcaa atcgcatcgt ggcgagtacg
       61 aagcgccgcc aaacgctcaa gcactttggt tgcgaattcg aaatgttatc gaagccgatg
      121 ggggtagacg agctgcaacg attgagcctc gcaaatcgtt tctaggcggc ttaatgggtc
      181 gcagctggca gttgtctcta ccacaacttg tatcatcggt agtagcgata gtcgtcctcg
      241 tatcattagc tacagtcgtg ggtatccgtc gttggggaat ggctcctgcc gcgatgcaga
      301 cgatgcaaat cgaaccaaca aatcccggtt cgagtgtgaa tgatcgcgtg tggcagcgtc
      361 agcaagtcat tagctattgg aatcagcgta tcgaagtaaa caagactcgt tggagtcctg
      421 aaatgcggga gacttttgat cgaaacatgg cagtaatcga tcaggctgtt agtgaatcga
      481 tgaatgaatt gaaccgcaat ccccatgatg atatcgctga gcaaatgttg aacgaatcac
      541 tcaacgataa actgacattg ctcaaggagt tttccgatct gtgataacgg cgctctaacc
      601 gccaactcac tgcggatcga ggaattttgg aaaagccccg ctgatgaacg ctaaattctc
      661 aacttggtct gtgtcttccc tggccaaact catttcaatc tttgtaatcg gctctctgtt
      721 ggggaccgtt gcagcgcagt cgcctcaacg actatcaaag cgttacacgg ttgggaaaaa
      781 tgttcgagtc gagttgaaaa acgtttccgg tacgatcgaa gttgaatcat ggaatcgcga
      841 tgagatccgg ctgtcggcca caatcgaatc tcccagtgct catgtagttc cgaaacagat
      901 cgatcagagt cttgtgatcg atgttatgag cgataataaa gctcgcggtg acgttggaga
      961 tgtgaacttc aaacttcaag ttcccattag ttcatcagtt gacctggaaa cacgtcgagg
     1021 tcagattagc gtcagtaatc ttcgcggcgc atcagtacgc gctcacgttt catccgaagg
     1081 cgacatcaca ctatcaaaca ttgccgcgac tcaggtgatt gcacaaaaca ccattggcaa
     1141 tatcttcttc gacggcgagt tttctcgtgg cgggacttac gaattcaaat caaccaaagg
     1201 cgatataacg attcgtattc cgggaaattc agctttccgg atggtcgcta cttcgccggc
     1261 tcgaaaaatt aaacttgggg atttttggaa tgatggattc aagactcagg acggccgcaa
     1321 gtatgtcggg gatgttggag atggtcgttc ttcagtctcg gtaacaaatt tcaacggctc
     1381 tatcaccttt atgcgcagat agcggttgga ttgaattgca aaccgactga cggacgctcc
     1441 tcgcagcgtc cgttttttta ttttggcgcc ggcggatcta aaggtttttg attggctgac
     1501 ttatcgacgt ggtattctcc gagtcggagg aaaactgatt tgcgccatgc cagtaagctt
     1561 ctgttagctg ccatcacctc gttactcttt tccattccag ctgcgagtca aactacttct
     1621 caaaaagata agtctcaggt ccttccaacc gcacctttgt taacaaggac cgccactcgt
     1681 cgagagtctc aacggttggg ttatggtgga actctaacgc ttgtgggagc tccgcgtggc
     1741 tcagtgacca tagaaggctg gtcaaaaagc gaagtcgagc taaccgccga tatagaactt
     1801 aaggctacca gcgaagaaga tttaaaccgc ctcgcgagca ttaatacttt cgtgtttgat
     1861 caggacttga atcatgtcag cgtgctcacc actggaacgc atgatcgcgt gtacatgaaa
     1921 aaattcaaga actttccgaa ggctctcctt aatcttccct ggaagatcga ctatcgaata
     1981 agagttccgg ccaccaccga tttggaagtt aacgctggtc atggtgcggt taaaatctca
     2041 ggagttgagg gtgcaatgcg aattagtgcc accgaaagcg atagctcgtt tactttaact
     2101 ggtggagtcg tatcggcaac tattgccgta ggcacagtaa cgctttcgat tccgactcga
     2161 agctggcgtg gtggaggggc ggacatacgc gtagcatcag gggctcttaa tgtcgagatt
     2221 cctccgggat ttagcggaga cattgacgcc gacgtcttac gaattggcca aattgtcaac
     2281 agctatgatg gtttagaggt gcgcgagaag ccagggatta cggaacgaac gattcgagcg
     2341 cgcgcgggag caggcggacc tttttttaag ttcaccgtcg gagatggaat tcttgcgatt
     2401 aagaagacgc cttcgtgaag gttcacgtcg ctcctggggc tttgacttta ctccatcccc
     2461 gactagaatg agcgctcgtt tttcgcatct ccaccacatc ttttagagac gtcacaatga
     2521 tcaaatccga caaatggatc cgtagaatgg ctgaagagca cgggatgatt gatccgttcg
     2581 aacctggcca ggtaaaagaa atagatggac gacgaatcgt gtcgtatggt acgtctagtt
     2641 acggctacga tatccgttct gcggacgagt tcaaattatt tacaaacatt aatagtacta
     2701 tcgtagatcc caaggaattt gattcaaaaa atttcgtcga tgtgaaatca gattccgtca
     2761 ttatcccgcc aaatagtttt gcacttgcgc gaaccgtcga gtacttccgc attcctcgaa
     2821 atgttctaac tatctgtttg ggaaaaagta cttatgccag gtgtggtatt ctcgtaaacg
     2881 tgacgccctt tgagcctgag tgggagggct atgtcactct cgagttttcc aatactacac
     2941 cgctgccggc caaaatttac gctaacgaag gagtcgctca agtcgtcttc tttgagtctg
     3001 acgaagtttg cgagacttcc tataaagacc gcggcggaaa ataccagggt caacgcggcg
     3061 ttactctacc taagctttaa ttttcctcaa tgtcgattcc acaacgctcc gtttcaacgg
     3121 gtgaattgca cttcaaaaag cttgactcgc gggcgactct gccagtgcgg ggaagcgtgg
     3181 catctgcggg gctggatcta ttttgtatcg aggatcttcg actcgaaccg aaggcccgaa
     3241 tactcgctcg aaccggaatc gccgtcgcaa tccccgtcgg ttactacggt cgtgtggcgc
     3301 ctcgatccgg actcgccgtc aagaatggca tcgatgttct agctggcgtt attgattcag
     3361 actatcgtgg tgaactttgt tgtgccctcc tcaatactgg cgatgaagcg gttgtattgc
     3421 cccagggtag tcgtatctgc caactcataa ttgaacgaat aattactcct gaaccgatct
     3481 gggtcgaaga cctggataac acttctcggg gcgatggtgg ctttggttct accggttgaa
     3541 tcgcccaaac ttctaaatta cttccgaacc tctgcaccaa tgattactca ccggaattct
     3601 ctaaaaaaat gcgcgctcat cctttcgttt gtagttttaa ccggaactgt cctgggacag
     3661 tctcttccgg ttgcgccaac gacctcgctt tccggttcgg aacgcgagct atcggggaaa
     3721 gtacgaaccg acacaatccg cgcgattgtt acagcactct ccgctgatga catgcaggga
     3781 cgtggtacag ctcaacccgg cggagacaag gcggcccagt ttcttgcgga taaattcgcc
     3841 aaactgggtc tcaagcccct tggagataaa ggttcatatc tccaatcaat aaattttcgt
     3901 gagattcaat tcttgagtga gacctcacta aaggtgggca cagagaatct gaagttaggc
     3961 caggactttt ttatcactcc gccctacagc ggcgataaaa acgtaaaggg cgatctagtg
     4021 tttgtggctt atggagtatc gacaccattt ttgaaacgga atgatttgca aggtatcgat
     4081 cttcgcggca aggtagccat cgtcctggaa ggtccgccac cagaagttaa caaggactcg
     4141 tggaaacaag cacacgctca atcgatggtt ctgcgcgggc ttatcgctcg aggtgcggcc
     4201 ggaataattc ttctcaacaa tggtccacag gaacatacgt ttgcggagct tgcagactac
     4261 ctgacaagac gccaaatcga attggctgac gaggctgaat tccctgattt cctgccacca
     4321 ttcttgaatg taagtgatgc aactgccgaa aagctttttc aggcctctgg cattactaaa
     4381 gaacaagcat tagcgaaagc tgccggcgaa aacttcgctc caataaactt gcagcaatca
     4441 gcttcgataa cggtccgttt aaagaagagc cggggaacgt cgagcaatgt tgtaggagtt
     4501 ttagcgggga cggatccgct gttgaagaca gaagcgcttg tttactcagc tcattacgat
     4561 gcctatggat tgtcggccga caacaaggtc tatcatggag ccgcagacaa tgccttaggc
     4621 gtgggccaga tgatcgcgat cgctgaagtg ttatctaagg cgaagttgag acgctcagta
     4681 attttcatgg cgctcactgg tgaggagtat gggggttatg gctcgactta ctgggtcaag
     4741 aatcctacct ggaagattaa acaagttgca gccgacctga acatggatgg tatgggaacg
     4801 gaagtttacg ggcctgtgaa agtcgttgtg ggttatggag ctgagcattc ttcactcggc
     4861 tctactttga acgaggtggc ggcagcaagc ggtcttaaag taataccgga tccgatgcct
     4921 gacgaaaaaa gcttttatcg ttcagatcac tatttctttg tgaagcgagg cattccggga
     4981 attatgcttc tgggcgcacc agaaggggat tcgagcgttt ggattgatcg cctcaagaag
     5041 tgggagaagg ccgattatca tcaacccaca gatgtgattc gtcccgactg gcactgggat
     5101 ggtccaagta cgattgcaaa ggttgcattg gtgatgggac tacgcattgc gaatgctgac
     5161 gcgatgccat cctggttacc cagctcgatt tttaatcgtg agcgaggaac agataaaccg
     5221 gcgcctccgg aaccctgagc attttccatt tgtcatttat catttcccat ttttcatttt
     5281 gtgatcagta ggttggttct gaaacgggtg ctccaggata tttttgaaga atggaaaatg
     5341 gaaaatgtgt cttgagtctc tccagtccga gccgattgaa gccctcctac agcccgttta
     5401 gttcaaatca ctgcgcaaaa cgtctgtaag cgcccaatcg agttcccata tcgagccg
//
LOCUS       DASSFP010000338         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2035002, whole genome shotgun sequence.
ACCESSION   DASSFP010000338 DASSFP010000000
VERSION     DASSFP010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035002"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFH91_07500"
     CDS             <1..799
                     /locus_tag="VFH91_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862876.1"
                     /translation="APLQDAQGRKGALVVLEDVTARISLEEQLQQREKLSSIGLLAAG
                     VAHEVNTPLTGVSSYTQMLLGMFNETDPKHALLQKVRTQAERATNIVNNLLNFSRTGN
                     ATEFGETDIARVLDDTLQLLEPQLRRSQIRIVRSYENGAPLVYGNAGKLQQVFTNLIL
                     NARDAIPDGGQITLSTGAADDGSLMVEVADTGIGIDPENVAKIYDPFYTTKGVGRGTG
                     LGLAVSYGIVQEHTGRITVESTPGKGTTFRITLPTARARARLQAVGD"
     gene            957..2384
                     /locus_tag="VFH91_07505"
     CDS             957..2384
                     /locus_tag="VFH91_07505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862877.1"
                     /translation="MKPERRNARKGSVLVVDDEEIMREVLETLLNAEGYRVDLAKTGE
                     EGLEAYGRRSFDVVLLDVSMPGIGGLRALEEFLKMDSEAVIVMITAYATFDTAIAAWE
                     RGAFGCIRKPFQNEQITATIAAGIKRRRKEEERRTLRRAMSKAVERGEIIGRSDLMQE
                     VFRLVEQVAPARSTVLITGESGTGKELIAKAIHEASPRAGRPFVTVNSSNIPSELLES
                     ELFGHTRGAFTGAVAAKKGLFEVADGGSIFLDEIGDIPPETQVRLLRVIQEREFTPLG
                     DTTPRRVDVRIIAATNIDLKEAVRQGGFREDLYYRLAVVPIELPPLRDRLEDILPLVQ
                     HFTQKYNDENGRTVSEQIAPEVLALLEGYSWPGNVRELENTIERAVVIAPGDEISREC
                     LRPEISDPQSSAAASQSGASNAAVGDIGRGINFYEEVRRFEIDLIRRALEQTSGHQSR
                     AARLLGMNATTLNSKIKTYNINLRP"
     gene            2419..2886
                     /locus_tag="VFH91_07510"
     CDS             2419..2886
                     /locus_tag="VFH91_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6862878.1"
                     /translation="MKRRELDGCPITGALQMLGDKWTMLVVRELASGPKRTMELLSSL
                     FPISSRTLVARLRDMENDNFIDRTDFGGNPPHIEYALTERGSLLLPLLDSLRQLGLRL
                     GCNDCHDRRDRQGSYCESCPFNQYSREIEAPSFNQRSSNSPSRRQQDDSIILL"
     gene            complement(2895..>3114)
                     /locus_tag="VFH91_07515"
     CDS             complement(2895..>3114)
                     /locus_tag="VFH91_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862879.1"
                     /translation="QDSVYRQAITAAGTGCMAALDAERFLDHLPVAMPSGEEVTIEGE
                     HVTADHTAIITPDGHMLPNQAELGAKSE"
BASE COUNT          767 a          767 c          836 g          744 t
ORIGIN      
        1 ggctcctttg caggatgccc agggtcgaaa gggcgcgctt gtggtactgg aagatgtaac
       61 tgcccgaata agccttgaag aacagctcca acaacgagag aaattatcgt cgatcggact
      121 gctggccgct ggggtcgcgc acgaggtcaa cacgccactg accggagttt cgtcatacac
      181 tcaaatgttg ctgggaatgt ttaatgagac agatcccaaa catgctttgc ttcaaaaagt
      241 acgaactcag gcagaacgcg ctaccaatat cgtcaacaat cttctcaact tttcgcgtac
      301 cggaaacgcc accgagtttg gagagaccga cattgcgcgc gtgctcgacg atacgctgca
      361 attactagag ccacaattgc gacgcagtca gattcgcatt gttcgttcgt atgaaaacgg
      421 agcaccgctg gtctatggaa acgccgggaa acttcaacaa gtctttacca acttgattct
      481 caatgcgcga gatgcgattc ccgatggtgg tcaaattact ctttcgaccg gcgcagcaga
      541 tgacggctcc ttgatggttg aagtcgctga cacgggtatc gggatcgacc cggaaaacgt
      601 cgctaagatc tatgatccgt tctacacgac taaaggagtt ggacgcggaa ccggcctcgg
      661 ccttgcagtc tcttacggaa tagttcagga acacacaggc cggattaccg ttgaaagcac
      721 gccaggtaaa ggaacaactt tccgtatcac actgccaacg gcgcgagctc gcgctcgatt
      781 gcaggcagtc ggggattgac cggtacccca ttcaattgat gcaaacagcg aacgcgattt
      841 gactatttcg actcaagaca gtttttattc gggttaggcc tgccgctgtc aggccttttg
      901 ttttgtcgaa ctaactcgtt aaactaaaca cactattctg ataccaaatg gatttgatga
      961 agcctgaaag acgaaatgcc cgcaagggtt cagtactcgt cgttgacgat gaagagatta
     1021 tgagagaagt gttggagaca cttctcaacg ctgaaggata tcgagttgac ctcgcaaaaa
     1081 ccggcgagga aggtctcgaa gcttatggcc gtcgatcttt tgatgtggtt ttgcttgatg
     1141 tgtcgatgcc gggtattggt ggactgcgag cgctggaaga gttcttgaaa atggattctg
     1201 aggcggtgat cgtaatgatc actgcttacg ccactttcga tacggccatt gcagcctggg
     1261 agcgcggcgc atttggctgc atccgcaagc cattccaaaa cgaacagatc actgccacca
     1321 ttgctgccgg tatcaagcga cgccgtaaag aggaagaacg acggactctg cggcgcgcga
     1381 tgagcaaagc cgtagaacgc ggcgaaatca ttgggcgctc tgacttaatg caggaggttt
     1441 ttcggcttgt tgagcaagtc gctcctgccc ggtctacagt tctaataacc ggcgagagtg
     1501 gcaccggtaa agagttaatt gccaaggcta ttcacgaggc aagcccgcgg gccggcagac
     1561 ccttcgtcac cgtcaatagt tcaaatattc cgtcggaact ccttgagtcg gagttgttcg
     1621 gacacacccg tggtgcattt accggagcgg ttgccgcgaa gaaagggtta tttgaagttg
     1681 ccgatggtgg ctcaattttt cttgacgaga tcggcgacat tccgcccgaa acgcaggtgc
     1741 gactcttacg agtcattcag gagcgggaat ttacaccgct cggagacacc actccacgaa
     1801 gagtggatgt gcgcatcatc gccgccacaa atattgatct aaaagaggct gtccgtcagg
     1861 gtgggttccg agaggatctc tattaccggc tcgctgtcgt tcctattgaa ttgcctccat
     1921 taagggatcg tctggaagat attctgcctt tggtccaaca cttcactcag aagtacaacg
     1981 atgaaaatgg ccgtactgtc tccgaacaga tcgctccgga agtgcttgcg ttgctcgaag
     2041 gttattcatg gcccgggaat gttcgtgaac tggaaaatac gatcgaacgc gctgttgtca
     2101 ttgcgccggg tgacgagatc agtcgcgagt gtttgcgtcc tgagataagc gatccgcagt
     2161 cctcagctgc ggcgtcgcag agtggagcga gcaacgccgc ggtaggagat ataggacgtg
     2221 gtataaactt ctacgaagaa gtcagacgat ttgagataga tctaattagg cgagcgcttg
     2281 agcagacaag tggccaccag tcacgtgcag cgcgcctgct aggcatgaat gcaacgacgc
     2341 tcaattcgaa gatcaagact tacaatatca acctacgtcc ataaggacgc acttttgatc
     2401 tgacgagatt tcccaaaagt gaaacgacgc gaacttgatg gctgcccgat taccggagca
     2461 ttacaaatgc tcggtgataa gtggacgatg cttgtcgttc gtgagttggc ttctggtcca
     2521 aaacggacta tggagcttct gagtagtctg tttccgatca gcagtcggac tcttgtcgca
     2581 cgtttgcgcg atatggagaa cgataacttt attgatcgaa cagactttgg gggaaatcca
     2641 ccccacatcg agtacgcatt gacggagcga ggatctttgc tgttaccact gttggattcg
     2701 ttacgccaac ttggactcag actgggctgt aacgattgtc atgatcgtag ggatcggcag
     2761 ggctcgtatt gtgagtcgtg cccttttaat cagtattcgc gggagattga agcaccttcg
     2821 ttcaaccagc gttcctctaa ttcaccgtct cgccggcaac aagacgattc gataatttta
     2881 ctctagtaaa ctcgttattc gcttttcgct cccaattcag cctgatttgg aagcatgtga
     2941 ccatctggag taatgatggc agtatggtcc gccgtaacgt gctctccttc gatcgtaact
     3001 tcttcaccgg agggcattgc gacgggcagg tgatctaaaa accgctcagc atctaatgct
     3061 gccatgcatc cagttccggc tgcggtaatc gcttgccggt acaccgaatc ctgg
//
LOCUS       DASSFP010000339         3972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_691276, whole genome shotgun sequence.
ACCESSION   DASSFP010000339 DASSFP010000000
VERSION     DASSFP010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691276"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..2428)
                     /locus_tag="VFH91_07520"
     CDS             complement(41..2428)
                     /locus_tag="VFH91_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6862880.1"
                     /translation="MTGLNCVRLSNIRRLIFSAIIALTIPCMLFSPPRSRSQNGTPDY
                     KNPRLTVDERVADLLKRMSVEEKVAQLVCLWQQRPQVQPQTDFSTDRGAVSSAKAEQV
                     MKYGIGQIARQRERKGPREGAEFANGVQKWLIENTRLGIPAILHDEILHGHMAQGSTS
                     FPQPIALASTWDPDFIGKVFTAAALETRARGSHQVLGPNLDVAREPRWGRTEETYGED
                     PYLISRMAVAAIKAIQGQGPTVDGDHVIATAKHFAAHGQPEAGTNIGPANYSERVLRE
                     VFLPSFEAAVKEAGIMSIMPSYNEIDGVPSSVNKWLLQDILRKEWGFKGYTVSDYYAI
                     PQLKDLHHVAADPRDAAKMSLEAGVDVELPDPDAFLTLVDLVKSKKVSEATLDAAVGR
                     HLRAKFLLGLFENPYVDPERAVKVTNSSANRGLAADAARRSIVLLKNDNNLLPLNLNT
                     LKSIAVIGPNAERVHLGGYSDNPERGVSVLQGIKDKTAGRVTVSYAEGCKITKEGGNW
                     WADTSTLSDAAEDLKLISQAVEVAKAADVALLVLGGNEDTNKEAWADNHLGDRDNLDL
                     VGRQNELVRQVLATGKPVVVMLINSGPLSINYVAENVPAILEGFYLGQETGVGVADVL
                     FGDYNPGAKLAVSFPRTVGQLPIYYNQKPTAKRGYLFANKDALFPFGYGLSYTTFSYS
                     QPTISPAKISTNGQAKVSVTVTNTGKRAGDEIVQLYIHDLVSSVTRPIKELKDFKRIS
                     LAPGESKNVEFVITPDKLWFYDLSMKRVVEPGWFDIMVGPSSAKYQTAKLEVVAQ"
     gene            complement(2509..3798)
                     /locus_tag="VFH91_07525"
     CDS             complement(2509..3798)
                     /locus_tag="VFH91_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862881.1"
                     /translation="MISLLENQATVGELLSDKARLRQEVESMVAKIPAIDIHTHVHPP
                     EFKSLYLSGIDDLLTYHYLIAETFRSTNLSYADFWRLTKAERADLVWKTLFVDNSPLS
                     EAARGIITILSAFGLDSRATNLSEARAFFNAQQPEDHINRVLGMSSVSELVMTNDPFN
                     EQEAKGWLSGLEHDSRFHASLRLDTLLNEWPIAHQYLAKQNFKVDIQLNANTFGEMRR
                     FLDEWILRIRPIYMAVSLPSDFAFPADDTRETLLREVVLPTASSHRLSLAIMIGVRRS
                     VNPALRSAGDGIGKADITAIERICAAYPDGKFLVTALSRENQHELCVAARKFKNLMPF
                     GCWWFLNNPSIVSEITTERLELLGPSFIPQHSDARVLEQLIYKWQHSRTVIAASLFEI
                     YERLLESGRAVTSAEIQRDVTRMFSGNFREWIAPASS"
BASE COUNT          939 a         1030 c          992 g         1011 t
ORIGIN      
        1 atgcccaacg caaataaacc agaacacctt gttcttcgtt tcactgcgct accacttcaa
       61 gtttagccgt ctgatacttt gctgaactcg gaccaaccat gatatcgaac caaccaggct
      121 ctactactcg tttcatgctg agatcgtaga accacaattt atccggagta atcacaaatt
      181 caacgttttt actttcgccg ggagccagcg atatccgttt gaagtccttt agctctttta
      241 taggtcgcgt gacggaactc accagatcgt gaatatatag ttggacgatc tcatctcccg
      301 cgcgttttcc cgtattagtg actgttacgc tgactttggc ttgtccattc gtcgagattt
      361 tcgccggaga aatcgtaggt tgcgagtagg agaaggttgt atagcttagc ccgtaaccga
      421 aaggaaacag tgcgtccttg ttggcaaaga ggtaaccgcg tttggcggta ggcttctggt
      481 tataatagat cggcagttgc ccaaccgttc gaggaaaact tacggccagc ttagcaccgg
      541 ggttatagtc accaaacaag acgtcggcga ccccaactcc cgtctcctga cccaggtaaa
      601 aaccctcaag aatagccggt acattctctg cgacgtagtt gattgacagt ggcccactat
      661 tgatcaacat caccacgacc ggttttcctg tcgcgagcac ttgtcttacc aactcgtttt
      721 gccggcctac aagatccaga ttgtctcgat cacccagatg attgtcagcc cacgcttctt
      781 tgttggtatc ttcgttgcca cccagaacca ataaagcgac gtcagccgcc tttgctactt
      841 cgactgcttg tgagatcaat tttagatctt cagccgcgtc actcaaggtg gacgtatcag
      901 cccaccagtt gccgccctct tttgtgatct tgcacccttc cgcgtaagag actgtgactc
      961 ggccggcagt tttgtcctta attccttgca acacgctaac accacgttcc ggattgtcgc
     1021 tgtagcctcc gaggtggacg cgttcggcat ttggcccaat cacagcgatc gacttcaacg
     1081 tgttgagatt gagagggagt agattgttat cgttcttcag cagcactatc gaacgccgcg
     1141 ccgcatcagc agcaagccct cggtttgccg atgaattggt cacttttaca gcgcgctcag
     1201 gatcgacgta aggattctca aagaggccga ggagaaactt tgcgcgcaga tgtcgtccaa
     1261 ccgccgcatc aagcgttgct tcagaaactt tctttgattt gacgaggtca acgagtgtta
     1321 gaaaagcatc gggatccggc aactcgacat ctactccagc ctctaaggac atttttgcag
     1381 catccctggg atcagctgcg acatgatgta aatccttgag ttggggaata gcatagtagt
     1441 cagacaccgt ataacccttg aagccccact ccttgcgcaa aatatcctgc aagagccatt
     1501 tattgacaga ggaaggcacg ccgtcgatct cgttatagga gggcatgata ctcatgatgc
     1561 ccgcctcttt aacggcagcc tcgaagcttg gcaaaaacac ttcgcgaagc actcgctcgg
     1621 agtaattggc aggaccgata ttcgtcccgg cttcgggctg accatgtgcg gcgaaatgtt
     1681 tggcagtggc gataacgtgg tcaccgtcga cagttggtcc ctggccctga atcgctttga
     1741 tcgctgcgac agccattcgc gaaatcaagt aaggatcttc accataggtc tcttcagtac
     1801 gtccccatct tggctctctg gctacatcga gattaggacc aagcacctga tggctgccac
     1861 gagcccgagt ctcgagggct gcggcggtaa agactttgcc gatgaaatct ggatcccagg
     1921 tgctcgcgag cgcaataggc tgcggaaagc tagtcgagcc ctgggccatg tgaccatgca
     1981 ggatttcatc gtgcagaatt gcgggaattc ccagtctggt attctcgatg agccactttt
     2041 gcacgccgtt ggcaaactcg gctccttcac gtggtccttt cctttctcgt tgtcgagcga
     2101 tctggccgat gccgtatttc ataacctgtt cggctttagc agatgacaca gctccacggt
     2161 ccgttgagaa atctgtttgc ggttggacct gtggtcgttg ctgccaaagg cataccagct
     2221 gagcgacctt ttcctccacc gacattcgtt tcaggagatc tgctacccgc tcgtcaacag
     2281 tcaaccgagg attcttgtaa tcgggcgtcc cattttgaga acgactgcgt ggcggactga
     2341 acaacataca gggaattgtt aaggcgatga tcgccgaaaa aatgagacgt ctgatgtttg
     2401 atagacgtac gcagtttaga cctgtcatgg ttgcgagatg ctcctgaact tttggggaaa
     2461 tgaaatagcg atttgaagat tgccgccttt tcttcacaaa cgattgcgct aagaagacgc
     2521 cggcgctatc cattcacgga aattacctga aaacattcga gtgacgtcgc gttggatctc
     2581 agctgaagtt actgcgcgtc cactctcgag caagcgctca taaatctcga acagtgaagc
     2641 cgcaatcact gtgcgcgaat gctgccactt atagatgagc tgctcaagta ctctggcgtc
     2701 ggagtgctgc ggaataaagc ttggtccaag tagttccaaa cgctctgtcg taatttcgga
     2761 tactatcgag ggattgttta gaaaccacca gcagccaaaa ggcatcaggt ttttaaactt
     2821 cctggcagca acgcagagct catgttgatt ctctcgcgac agtgccgtta cgaggaactt
     2881 accatcggga tatgcggcac aaatgcgttc gattgccgtg atatctgctt tcccaattcc
     2941 atcacccgcc gaacgtaggg ccggattcac actgcgtcta acaccaatca tgatagccag
     3001 tgataatcga tgtgagcttg cggttggtag cacaacctct ctcaatagcg tctcccgcgt
     3061 atcgtcagcg ggaaaggcaa aatcagaagg taacgacact gccatatata tcggccgaat
     3121 tctgagaatc cactcatcga ggaagcggcg catttctccg aaggtgtttg cgtttagctg
     3181 gatatcaact ttgaaatttt gtttcgccag atactggtga gcgatgggcc actcgttcag
     3241 caaggtgtcg aggcggagcg atgcatggaa gcgtgaatcg tgttcgagtc cactcagcca
     3301 gccttttgct tcttgctcgt taaatggatc gttggtcatt actagttcgc ttaccgatga
     3361 catcccgagt acacgattga tgtggtcttc gggttgttga gcattaaaga atgcgcgtgc
     3421 ttcgctcaaa ttcgtcgcgc gtgaatcaag tccgaaagct gacaggattg tgataatacc
     3481 gcgcgcagct tctgacaaag gtgagttgtc aacaaataag gtcttccaga ccaggtcagc
     3541 ccgttcggct tttgtcagcc gccagaagtc agcatagcta agattagtag accgaaaagt
     3601 ttcggcgatc aagtaatgat aagtgagcag atcatcaatg ccggaaagat agagcgactt
     3661 gaattctggc gggtgcacat gggtatgaat atcgatagcc gggatctttg cgaccatgct
     3721 ttctacttcc tgacgcagtc gcgccttgtc tgaaagcaac tcgcccacgg tggcttgatt
     3781 ttctaagagg cttatcatta ctccgtccct ttctcggttt aatcaaaggc tgatgtcggg
     3841 cgtcctggaa tcttttggat cggcccaacg atcatttgga atcgtcggcg cccagcccgg
     3901 tctcagcggg tcctggtcgt acggatatga gcccaaacgg cgatagagtt cgttataccc
     3961 gcccaacgag tc
//
LOCUS       DASSFP010000340         6379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1128148, whole genome shotgun sequence.
ACCESSION   DASSFP010000340 DASSFP010000000
VERSION     DASSFP010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128148"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VFH91_07530"
     CDS             complement(<1..850)
                     /locus_tag="VFH91_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET6862882.1"
                     /translation="MTTPESPESTLAPPSGDMPKEEFRRLGHQLIDWIAEYFDQIEEF
                     PVLSKNEPGELKSKLPAAAPKSGESMESILKDIDQLIVPALTHWSHPSFFAYFATSTS
                     APGIFGELLAAAFDNKAMLWRTSPASTELEEVVLDWLRQMMGLDPGLSGIIYDTASVS
                     SMHAIAAAREGVEQRIREDGMSGRPDLPLLRVYVSEQAHSSIEKGVITLGLGQRGIRK
                     IPTDSAFRMDPKALSEAIVEDKRNGYLPFCIVATVGTTSTASVDPISDIIPIGEEHAL
                     WLHVDAA"
     gene            1551..2777
                     /locus_tag="VFH91_07535"
     CDS             1551..2777
                     /locus_tag="VFH91_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6862883.1"
                     /translation="MKRIAISLLLVTSLLFALACERKGGAGASNGSGPIVVGYYGDLS
                     GRTSNFGQSTKEGVLMAADEINKAGGLNGRQVQILSEDDEGRPEKAATVVTKLIDQDK
                     VVALLGEVASGNTLAAAPKAQGAKVPMISPSSTNPAVTQVGDYIFRVCFIDPFQGEVM
                     AKFAANTLKAKKAAIMLDFNSPYSRGLTEFFEASFTRLGGQIVNKQSYTQGDRDYKGQ
                     LTAIRSASPDVIYVPGYYGEVGVIAKQAQQLGIKAPLLGGDGWDATQLWELGGDALNG
                     DFISNHYSVDDPSPAIQKFVADYHGRYSKRPDALAALGYDAMKVLAEGIRKAGTTDGP
                     KLRDAIAQTKDFPGVTGTISLDAQRNAVKPAVVLKLQDKKYIYQETIYPEGMSPQTKA
                     APAATAEATVGSKAGN"
     gene            2802..3719
                     /locus_tag="VFH91_07540"
     CDS             2802..3719
                     /locus_tag="VFH91_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6862884.1"
                     /translation="MDTFVQQLINGLTIGAIYALIALGYTMVYGILRLINFAHGDIYM
                     VGAFAGFFLATQLGFAAGPSLVGLVIVLIGAMIIAALFGMAIERFAYRPIRKYARMTT
                     LITAIGVSLLIENSFVAKLGAAPRAFPQLLQEQSYTLFGNAAISKSQILIFAVSIFLM
                     LLLQWIIFKTKVGTAMRAVSFNLNSAKLMGINTDMIISVTFALGSALAAAGGVLTAQY
                     NPQIDPLMGIITGLKAFVAAVLGGIGNIPGAVLGGLLIGLAETFVVGYGQNVGVPTTF
                     RDAVAFAILILVLLFRPSGLLGSTVQEKV"
     gene            3755..4846
                     /locus_tag="VFH91_07545"
     CDS             3755..4846
                     /locus_tag="VFH91_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6862885.1"
                     /translation="MPILVKRLLAAFVIVLLLIAINQYMSDEGLFGFGVPPYYGRVIM
                     LAGINVILAVSLNLITGFTGQFSIGHAGFMAVGAYTSAYLTVYHAQTWEHSLAGVIGV
                     TAAHALIFLFAIVVGGITAALAGLLVGIPSLRLRGDYLAIVTLGFAEIIRIVILNIDK
                     VGGATGFTVPGYTNFLWVGLFAVLTIVVVYNIVHSDMGRALVSIREDELAAEAMGINT
                     TRYKVLSFVISSALAGMAGVLFGHYTQFLSTNDFQFIRSFEIIIMIVLGGMGSLTGAV
                     LGAIVITIMPELLRRLPGDLSQYRLVVYSALLILIMLTRPQGILGTREFGLSWLKRPQ
                     RRPEGDKPVGEKGVPIAEQPKTTSSEGKS"
     gene            4846..5643
                     /locus_tag="VFH91_07550"
     CDS             4846..5643
                     /locus_tag="VFH91_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862886.1"
                     /translation="MSGKMDAQPVQNGNAVLKTRLCTIQFGGLVAVNCLDMNVSAGEI
                     YGLIGPNGAGKTTIFNLLTGVYKPTSGEIFFQGQRVDGLKPYEICRRGVSRTFQNIRL
                     FPSMSVLENVMTACHRHAQQNLFDAVLRLPRFERDEREHREFAMELLEIFDLARFKDE
                     GGTSLPYGNQRRLEIVRALATRPQLLLLDEPAAGMNPSEQEDLMRLIKEIRDRFSLTI
                     LLVEHNMKVVMGVCERIQVVDYGKSIALGLPEEIKNDPKVIEAYLGD"
     gene            5693..>6379
                     /locus_tag="VFH91_07555"
     CDS             5693..>6379
                     /locus_tag="VFH91_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6862887.1"
                     /translation="MLTLENVSVNYGAIEALTGISLHVNAGEVVTLIGANGAGKTTTL
                     RTITGLLQPRAGQINFEGEEIGGKPTHKLVARGISMSPEGRGVFANLSVRENLQMGAY
                     LNKNKKSISEGMDQAFRMFPRLKEREAQKAGTLSGGEQQMLAIGRALMSNPRLLLLDE
                     PSLGLAPLVVHTIFEAIDEIKSKGTTILLVEQNAHAALGHSDRAYVLETGRIVMEGPS
                     KQLAADPRIKE"
BASE COUNT         1544 a         1607 c         1634 g         1594 t
ORIGIN      
        1 aggccgcatc aacgtgtaac cagagtgcgt gttcttcacc gattgggata atgtcgctta
       61 ttggatcgac actcgccgtc gaagtcgtac caaccgtcgc tacaatgcaa aatggtaaat
      121 aaccgttacg cttgtcttca acgattgcct cggaaagcgc tttgggatcc attcgaaatg
      181 cactgtcggt cggaatcttg cggattccac gttggcccaa cccgagggtg ataacacctt
      241 tttcaattga ggagtgcgcc tgttctgaaa cgtaaacccg caacaacggc aagtctggtc
      301 tgccactcat cccgtcttcg cgaatgcgtt gttctactcc ttcgcgggca gcggctatcg
      361 catgcatgct ggaaacagac gctgtgtcgt aaatgatgcc gcttagacca ggatcaagac
      421 ccatcatttg tcgcaaccaa tcgagaacaa cttcttcgag ttcagtcgag gccggtgatg
      481 ttcgccagag cattgccttg ttgtcgaagg cggcagctaa tagttcacca aaaatccctg
      541 gagctgaggt cgacgtcgcg aagtatgcga aaaaggatgg atgactccag tgagtcaacg
      601 ccggaacaat cagttgatcg atatccttca aaatcgattc catcgactcg ccggacttgg
      661 gagctgcggc gggcaatttt gatttgagct cgcccggttc atttttagat aacaccggaa
      721 actcttcgat ctgatcaaag tattcggcga tccagtcgat caactggtgt cctagacgtc
      781 gaaactcttc cttcggcatg tctcccgatg gaggagcgag cgttgattca gggctttcag
      841 gggtagtcac agcccgcgta tcttacaggg tgaagattaa cgagcccaag gagacccggg
      901 gtcgatcgag gatggcgagt cgtaacatcg gtcaccaccg aagcgtcggg tgagtctcga
      961 aataaagtcg cgtcctaatc tgaaccttct ctgtgctgcc tctgagatct ctgtggctct
     1021 gtggtgatgg ttttcttagg actttgctca ccacatacaa ggcacagtgg acacaaaggt
     1081 tgcacagagt aagcaagtta ggacacaact cgaaataagg tcgtgggtca ttttcaagat
     1141 ttcttgtttc tctgaacagg gcacggatcc acgaagtcac acgaagcgtc acgaaacatc
     1201 ggtctttttt cgtgcagttt cgtgtaactt cgtggatcgt gctttttccc taatctataa
     1261 ggatcgacac tctgattgaa atcgacccac tactcgaaat aactagagct tgacgattca
     1321 aaagagcaga gctcgaagcc caaaacacca aattactgac tccaaagcac aaacctacaa
     1381 aaatgcagtt ctgaatttgc ggaaaatcag ccttaaagtt tatattcaga ctcgatttaa
     1441 ttagccgtgc catgcaaatc ggtctgctcc aagactgcgg cgacttattt caatctcaaa
     1501 aagttcggaa tcctatcgtt taaaacattt agtactctgt gaggaccact atgaaacgca
     1561 tcgcaatctc ccttctgctc gttacgagtc tgctgttcgc cttagcctgt gaacgcaaag
     1621 gcggagcggg agcttccaac ggttctggcc cgatagtagt cggctattac ggcgaccttt
     1681 caggacgtac ttccaatttc ggccagtcaa ccaaggaggg ggtgctgatg gctgctgacg
     1741 aaatcaacaa ggcagggggc ctgaacggcc gccaggtaca aattctttca gaagatgatg
     1801 aaggacgtcc cgaaaaagct gcgaccgtcg tcactaaact gatcgatcaa gacaaagttg
     1861 tcgcgctatt aggcgaagtt gcatcgggca acactcttgc tgccgctcca aaagctcagg
     1921 gtgcgaaagt gccgatgatt tcaccctcgt cgacgaatcc agccgttacc caggttggag
     1981 attacatctt ccgcgtttgc ttcatcgatc catttcaagg tgaggtgatg gccaagttcg
     2041 cagccaatac gctcaaagcg aaaaaggccg caatcatgct cgacttcaac tcgccctata
     2101 gccgcggact tacagagttc ttcgaagcaa gctttacaag gcttggtggt caaatagtta
     2161 acaagcagtc gtacacacag ggtgatcgcg attacaaagg tcagttgacg gcgatccgat
     2221 ccgcgagtcc tgatgttatc tatgtgcccg gttattacgg agaggtcggt gtaatcgcga
     2281 aacaagcgca acagttgggc attaaggctc cgctgctcgg tggcgatggc tgggatgcaa
     2341 ctcaactctg ggagctcggt ggcgacgcac tcaatggcga tttcatttcc aaccactact
     2401 cggttgacga tccttctcct gcgattcaaa agtttgttgc cgattaccat ggtcgttatt
     2461 caaagcgacc cgacgctctc gctgctctcg gttatgacgc catgaaagtt ttggctgaag
     2521 gtatcaggaa agctggtact actgacggac cgaagcttcg tgatgcaatc gcgcagacaa
     2581 aggattttcc cggcgttact ggaacaatct cactcgacgc tcaacgcaac gcggtgaaac
     2641 cggcagttgt tcttaaactt caggacaaga agtacatcta ccaggaaaca atctatcccg
     2701 aaggcatgtc accacaaaca aaagccgccc cagcggcaac cgccgaggct acagttggaa
     2761 gcaaggctgg aaattaggaa tccgtaagac cactgacaac tatggatacc tttgttcagc
     2821 agcttattaa tggtctgacc atcggtgcga tctacgccct gatcgcgctc gggtacacga
     2881 tggtctacgg catcttaagg ctcatcaact tcgcgcacgg cgatatctac atggtcggcg
     2941 cattcgcggg attctttctg gcaacgcaac tcggtttcgc ggcaggccca tcgctggttg
     3001 gactagttat tgttctgatt ggcgcaatga tcatcgctgc attgttcggt atggcaatcg
     3061 agagatttgc ctaccggcca atcagaaagt atgcgcgcat gaccacgttg attacggcca
     3121 ttggtgtctc gctactgatt gaaaacagct tcgttgcaaa acttggcgcc gccccgaggg
     3181 ctttcccgca actgttgcag gaacaaagct atacactctt cggtaacgct gccatctcaa
     3241 aatctcagat tcttattttt gcggtctcaa tcttcttgat gcttctcctt cagtggatca
     3301 tcttcaagac aaaagtgggt accgcgatgc gcgccgtgtc gttcaacctc aactctgcaa
     3361 agttgatggg gatcaacact gacatgataa tttcagtcac ctttgctctc ggctcggctc
     3421 tcgctgctgc cgggggcgta ttgaccgctc aatataatcc gcagatcgat ccgttgatgg
     3481 ggatcatcac cggactgaaa gcattcgtag cagcggtact cggaggcata ggaaatattc
     3541 ccggcgcggt actcggaggt ttactgatag gtctcgccga aacttttgtc gtggggtacg
     3601 gtcagaacgt tggcgtaccc acgacgtttc gcgatgctgt tgccttcgca attctgattc
     3661 ttgttctctt gtttcgtcca agtggcttgc tgggatcaac cgtacaggag aaggtttaga
     3721 ggtcgtaccg ggtcaacagc agccgtagat aacaatgccc atcttagtca aacggcttct
     3781 cgccgctttc gtcatcgtcc tgttactcat cgctattaac cagtacatga gcgatgaagg
     3841 tctgttcggg tttggtgtgc cgccatacta cggtcgcgtc atcatgctcg cgggaatcaa
     3901 tgtgattctc gcagtatcgt taaatctaat taccggtttc acgggacagt tttcgattgg
     3961 ccacgcggga ttcatggcgg tcggcgctta tacttcggcc tatttaactg tctatcacgc
     4021 gcaaacgtgg gagcattccc tggccggcgt cattggcgtt acggccgcgc atgcactcat
     4081 ctttttattc gccattgtgg tcggcggcat aacggcggcg ctggcgggcc tgctggttgg
     4141 aattccctct ttacgactgc gcggcgatta tctcgctatc gtcacgcttg gttttgccga
     4201 aataattcga atcgtaatcc tgaacatcga caaggttggc ggcgctaccg gttttaccgt
     4261 tcccggttat acgaacttcc tctgggtcgg actatttgca gttttaacta ttgtcgttgt
     4321 ctataacatc gtccattcag atatgggtcg agctcttgtg tcgattcgcg aagatgaact
     4381 tgctgctgag gcaatgggca tcaatacgac tcgttacaaa gtcctgtcgt ttgtgatcag
     4441 ttcagctctc gcaggcatgg ccggcgtctt atttgggcat tacactcaat tcttgagcac
     4501 caacgacttt caattcattc gatcattcga aatcataatt atgatcgtgc tcggcggcat
     4561 gggctcgctt acgggtgcgg ttcttggtgc gattgttatc accatcatgc ctgagttgtt
     4621 gcggcggcta ccgggtgatt tatcgcagta tcgtctggtt gtctattcgg ctttactcat
     4681 cttgattatg ttgacccgtc cccagggtat tcttggtaca cgagaattcg gtctttcatg
     4741 gttgaagcgt ccgcagcgtc gtcctgaagg tgataaaccc gtaggcgaaa agggagtacc
     4801 aatcgccgag cagccgaaga ctacatcaag cgagggtaag tcataatgag cggcaaaatg
     4861 gatgcacaac cggtccagaa tggaaatgca gtactgaaga ccagattgtg tactattcag
     4921 tttggcggct tagttgctgt caactgtttg gatatgaatg tcagcgcagg cgagatctat
     4981 ggattgattg gtccaaacgg cgccggcaaa acgacgatct tcaacttgct caccggcgtc
     5041 tataaaccaa catccggtga aatctttttc cagggtcaac gggttgacgg tttgaaaccg
     5101 tatgagattt gccgtcgtgg agtctcgcgc acgttccaaa acatccggct ctttcccagt
     5161 atgtcagtgt tggaaaacgt gatgactgca tgtcatcgcc acgcccaaca gaatctcttt
     5221 gacgcagtat tacgactgcc gcgctttgag cgtgatgagc gagagcatcg agaatttgcg
     5281 atggagctgc tcgagatttt cgatcttgct cgattcaaag atgaaggcgg tacaagtctt
     5341 ccgtatggca atcagcgacg cctcgagatt gttcgcgctc tggctacacg gccacaactc
     5401 ttgttgctgg atgagccggc tgccggtatg aacccgtccg aacaggaaga tctgatgcgc
     5461 ctgatcaaag agatacggga tcgcttcagt ctgacgattc tactcgtcga gcacaacatg
     5521 aaagtggtta tgggcgtgtg cgagcgaatc caggtggttg actacggcaa gtcgatcgca
     5581 ctgggtctac ccgaagagat caagaatgac ccgaaggtca tagaggctta tttaggcgac
     5641 tagagtcttt aatagttcaa tacgtcatat aagtcctata ccaaacctac aaatgcttac
     5701 cctcgagaat gtatcagtaa attacggcgc aatcgaagcg ttgaccggaa tctctttaca
     5761 cgttaacgcc ggagaagtcg tcacgttaat tggcgcgaac ggtgcgggta agactacaac
     5821 tcttcgcact atcaccggct tgctgcagcc acgcgctggt caaataaatt ttgaaggtga
     5881 agagattggc ggcaaaccga ctcacaagct tgtcgctcgc ggcatctcaa tgtcgccgga
     5941 aggtcgcggc gtttttgcaa atctttcggt gcgcgagaac ctacagatgg gcgcctatct
     6001 aaacaagaac aagaaaagca tcagcgaagg catggaccag gcttttcgaa tgtttccccg
     6061 tctgaaagaa cgagaggcgc aaaaggcagg cacactctct ggcggtgaac aacaaatgct
     6121 ggcgatagga cgcgctttga tgtcgaaccc gcgcctgctg ctcctcgatg aaccctccct
     6181 cggtctggcg ccgcttgtag tccatacaat cttcgaagca attgatgaga tcaaaagcaa
     6241 gggcacgaca atcctcctgg tagaacagaa tgcgcacgca gcgttgggcc actccgatcg
     6301 agcttacgtg ttagaaaccg gtcgcatcgt gatggaaggg ccgtcgaaac agttagccgc
     6361 tgatcccaga atcaaagaa
//
LOCUS       DASSFP010000341         6068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1583269, whole genome shotgun sequence.
ACCESSION   DASSFP010000341 DASSFP010000000
VERSION     DASSFP010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583269"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFH91_07560"
     CDS             <1..594
                     /locus_tag="VFH91_07560"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET6862888.1"
                     /translation="PVSEFLLRIFFVKLNSMTTKTHLMTADELIALPRGQHRYELVKG
                     ELLTMSPSGEEHGAVIGNLTILLGQFVKANYLGTLYGAETGFKLESDPDTVLAPDVAF
                     ISRNRVGERSKGYRLGAPDLAVEVLSPGDHKKKVERKTAQWLELGAKAVWLVDPQSKT
                     VEVHNANGEITVFRENEELVDLDLVPGFRALVSDIFA"
     gene            717..1610
                     /gene="murI"
                     /locus_tag="VFH91_07565"
     CDS             717..1610
                     /gene="murI"
                     /locus_tag="VFH91_07565"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET6862889.1"
                     /translation="MILTFVTEEQASLNRNLPIGIFDSGVGGLTVYRALHERLPNERF
                     VYLGDTARVPYGTKSLATVERYAVENAKFLETHGIKLLVVACNTASALALPAIRKAVG
                     IDVVGVIGPGARAAAALVKDGQRVGVIATEATVQSGAYARAIASVNPAIEVIEQACPL
                     FVSLAEEGWADSEVAHAVATEYLTDLKSKSISVLVLGCTHYPILRQTIADIIGLEAKL
                     IDSGEATAVEVVMRLEKLGLSHDQDVSRHGDRRLGDDLDHFYVTDAAQRFAKVAERFL
                     GSAPSILEAVEVWGNDELRTD"
     gene            1591..2322
                     /gene="rph"
                     /locus_tag="VFH91_07570"
     CDS             1591..2322
                     /gene="rph"
                     /locus_tag="VFH91_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET6862890.1"
                     /translation="MSFERTDGRAADELRPIKITPDFLAYAEGSVLIEMGNTRVACAA
                     TLEDRVPPFLRNTGQGWVTAEYAMLPRATQTRTARETGRGGPSGRTHEIQRLIGRSLR
                     AVSDMKAFGERTFTIDCDVLQADGGTRTAAITGAYVAFALATKRVIKSGRLSRQILTN
                     SVAAVSVGIVNGTPLLDLKYDEDSRAEVDMNIVCTGDGKFIEVQGTAEREPFSRSQMN
                     ELVTLAEQGIATIVKIQQEALLLVV"
     gene            2428..2934
                     /locus_tag="VFH91_07575"
     CDS             2428..2934
                     /locus_tag="VFH91_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862891.1"
                     /translation="MRVFLVLSLLVVVLMSSVVLAQEVWTHDKVDYSFEIPSPTWRAI
                     SEPDAAHEHPEFVYGDRLDGYLTVRKEVVEAGTTAAELARRDQDQKLRFLPGFVEGKQ
                     ETFNGRLDGVTISYEFTRTGKQMLGRTYYLQADNRTIYALRFTGLRDKLGRIRNQTDL
                     IARTFKLK"
     gene            3127..3543
                     /locus_tag="VFH91_07580"
     CDS             3127..3543
                     /locus_tag="VFH91_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET6862892.1"
                     /translation="MKLQLAPGLTIRRSTIHGKGCFATQNFGRRRKIAEYAGEKITNA
                     EARRRAHRKTLRICEVNSRFSLDGSRGGNGTHYINHSCKPNAFMQILYDHILFIALRD
                     IQEGEEITIDYESTLHSDTKRCICGSPDCRGTINRV"
     gene            complement(3638..5647)
                     /locus_tag="VFH91_07585"
     CDS             complement(3638..5647)
                     /locus_tag="VFH91_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862893.1"
                     /translation="MIRLRPLFADITAAGVSIIAALLTFNLWYIGVLPPLAPIFFKFF
                     SIESGYLVSPEPKVALAALIVFSVAIGLVIHRLSFGKAWPFVAGALLLANFICLLIAR
                     FFSTDLLLTSLLTTGVLTVILLQGFRLWRVDLKIARTLLNSPKALGQTEGRAAATRLS
                     SGLKLLTSVLPLDEAVVFKCLESNSLKTIARIKSRRYAAQEARNSVWRDGIRLCDKAI
                     ATGKLLADISNDGTSSKIAVPLRMEQEIVGVLLVRIAGRFCDEDKALLEAVGSQFARN
                     LKREEFLQNPARSQAFSFFSHTGSQRKLEGLYVLKALTTEQRCEANALSDINDGIAIA
                     YLDGTIALTNSPLLKFANLEAEQAKSLDLFGLLDHFRTDVFDEPIIAVRRVLQTGIAY
                     EGELRFEQKNMILGMRISLLRESEDALGGSRDPIGLVIHVKDLTQMKEYNKLKSDMIS
                     LMSHELRTPLTSINGFAEILTGDNTLPEQAREFVGIIASESQRMSRMINTFLAVTQLE
                     RKDKQEVLKIPLRLDEVVRDTIASMQSLAKKKRIRLIEQPAQRIPPVAADKGLITQAV
                     KNLVNNAIKYSPERTTVTVSTALEAEAVRVSVEDRGFGIPSESLDRVWEKFYRVVRDG
                     QEKDDESTGLGLSFVREVVEQHGGNVAVDSEVGRGSRFSFTLPRL"
     gene            complement(5758..>6068)
                     /locus_tag="VFH91_07590"
     CDS             complement(5758..>6068)
                     /locus_tag="VFH91_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862894.1"
                     /translation="KARALKLIERIENPAVRVELMSGESAIGGGAGPTSMIKTSLLAI
                     SHDSFGAQEILNSLRTSNPPVIARISADKVLLDLRTVLEEQEELLLLALNLLLRQEKL
                     "
BASE COUNT         1523 a         1493 c         1554 g         1498 t
ORIGIN      
        1 ccggtttcag aattcttgtt aaggatattt tttgttaagt tgaacagcat gacgactaag
       61 actcacttaa tgaccgcaga cgagttaatc gcattgccac gcgggcagca tcgttatgaa
      121 cttgtcaaag gagaactcct cacaatgtct ccatcaggtg aagaacatgg cgcagtcata
      181 gggaatctca ccatattgct aggccagttc gttaaagcta attatctggg cacgctttat
      241 ggagcggaaa ccgggttcaa acttgagagc gatcccgata ccgttctcgc tcctgacgta
      301 gcattcatta gtcgcaatcg tgtgggcgag cggtccaaag gttatcgctt aggtgcgccc
      361 gatttggccg tagaggtact ctcaccaggc gaccacaaga agaaagtcga aagaaagact
      421 gctcagtggt tggaacttgg cgcgaaagct gtttggcttg ttgaccctca atcaaaaact
      481 gtggaagttc ataatgcgaa tggtgaaata actgtcttcc gtgagaacga agaactagtc
      541 gacctcgatc ttgttcctgg ttttcgcgca ctcgtttctg atattttcgc ctagctcgag
      601 gtcagcacca caacgcggta gcgtgtggct ctattgcggt catcatcgta ttgagggacg
      661 tcacacgcta ccgcgttgtg gtactgaccc cgagctaagc ggcacactag cgcgttgtga
      721 tattgacttt tgttaccgaa gaacaagctt ccttgaatcg aaatcttcct atcgggatct
      781 ttgacagcgg agtcggcgga ctaaccgttt atcgggccct tcacgaacgg ttaccgaacg
      841 agcgttttgt ctatttaggc gatacagctc gtgtgcccta cggaaccaag tctcttgcca
      901 ccgttgagcg ctatgcagtc gagaatgcaa agttcctgga aactcatggc ataaaactct
      961 tagttgtagc ctgtaataca gcctcagcgc tggctctacc ggcaatccgg aaggctgttg
     1021 gcatagacgt cgtaggagtg attggcccag gagctcgcgc cgcagccgcc ttggtaaagg
     1081 atggccagag ggttggcgtc atagcaacgg aagcaacggt gcaaagtggc gcttatgcaa
     1141 gggctatcgc gtctgtcaat ccagcaattg aagtaattga acaagcgtgc ccgctttttg
     1201 tgtcgttagc cgaagagggg tgggccgact ctgaagtggc ccatgcggtg gcgaccgaat
     1261 atcttaccga tctgaagtcg aagtcgatca gtgtgctggt gcttggttgc actcattatc
     1321 caattctgcg tcaaacgata gccgacatca ttggactaga ggcaaaactg atagactcag
     1381 gagaagcgac cgctgtcgag gttgtaatgc gtttagaaaa acttggcctt tcgcatgatc
     1441 aggatgttag ccgtcatggc gatcgtcgcc ttggagatga tctggaccat ttttatgtaa
     1501 ctgatgcggc gcagagattt gctaaggtag cggagcgatt tcttggttca gctccttcaa
     1561 ttctggaagc agttgaggta tggggaaacg atgagcttcg aacggactga tgggcgtgct
     1621 gcagacgagc tgcgtcccat taagataaca cctgatttcc tggcgtacgc tgaaggctcg
     1681 gtattaatag aaatgggcaa taccagagtt gcgtgcgcag ccaccctcga agatcgagtt
     1741 cctccatttc ttcgaaacac cggtcagggc tgggtcaccg ctgagtacgc tatgctaccg
     1801 cgagcaactc aaactcgaac tgcccgcgaa acgggtcgcg gtggtccctc gggacgtact
     1861 catgagattc aacgcttgat cggaagaagt ctgcgagcgg tttcagacat gaaagcgttt
     1921 ggggaacgta cctttacgat tgactgtgat gtactgcagg ccgacggtgg gacgcgtaca
     1981 gctgcgatta cgggtgcata cgtagctttt gccttagcta ccaagcgcgt gatcaaatcc
     2041 ggtcgcctca gccgtcaaat tctcaccaac tcagtagcgg cggtgagtgt tggaatcgtg
     2101 aatggcacgc cgttgctgga cttgaagtac gacgaggatt ctcgagccga agttgatatg
     2161 aacattgtgt gcactggtga cggcaaattt attgaagttc aaggtaccgc tgaaagagag
     2221 ccgttcagcc gcagtcagat gaatgagttg gtgacgctcg cggagcaagg gattgcaacc
     2281 atcgtaaaga ttcaacaaga ggcgctttta ttggtagttt gaaaaaggac aactgcgaaa
     2341 tcggctgccg gcatctgata cctggcttgt aaggcgccgt tcagtatttg cgctgattgt
     2401 cagttaaata aggagtcaat agtccaaatg cgagtgtttc tggtcttgag cctgcttgta
     2461 gtcgtactga tgtcgtctgt cgtattggcc caggaagttt ggactcacga caaagttgat
     2521 tattcctttg agattccatc gccgacatgg cgcgctatct cagaaccgga tgctgcgcac
     2581 gaacaccctg aattcgtgta tggcgaccgg ctcgacggtt acctcaccgt gcgcaaagaa
     2641 gttgttgagg ccggtaccac cgccgctgaa ctcgctcgcc gggaccagga tcagaaattg
     2701 aggtttttac cgggctttgt cgagggtaaa caagagacct ttaatggccg gctggatggc
     2761 gtgactatct cttatgaatt tacgcgtacc ggaaaacaaa tgctcgggcg cacttattat
     2821 cttcaagctg acaaccgcac gatctacgcg ctgcgtttta ccggccttcg agacaagctg
     2881 ggtcgaatcc gcaatcaaac cgacctcata gcgcgcacct tcaaacttaa gtaaacgcct
     2941 ctctcaaatc ttctgtaatg ccagaagcaa aaggccaaca ataggtatgt ctcgagccat
     3001 taacctcacg taactgtgaa ataacagttc aaccgcaact cttcaatttc cttctcgtct
     3061 cagcgtcaac acaaagtgat agactgcacg acgttttaat aacctcgact gcaagataga
     3121 cctcaaatga aactccaact cgcgcccggc ttaacgatca ggcgctcaac aatccatggt
     3181 aaaggttgtt tcgccacgca aaatttcggc aggcgtcgaa aaattgccga gtatgccgga
     3241 gaaaagatca ccaacgctga ggcccgtcga cgcgctcatc gaaagacgct gcgaatctgt
     3301 gaagtaaaca gtcggtttag ccttgatggt agccgcggag gaaacggcac tcattacatc
     3361 aatcattcat gcaagccgaa cgcgtttatg cagatacttt acgaccacat tctttttatt
     3421 gcgctacgcg atattcagga aggcgaagag attacaattg actacgagtc aactttgcac
     3481 tcagatacaa agcgatgcat ttgtggctct cccgattgtc gcggaacaat caatcgggtt
     3541 tgagttagtc cgtagtcagt ggtccgtggt ccacgttttc ggctgcgcca atcacaacgg
     3601 accactgaca acggaccact gacaactgac aacgaattta tagacgtgga agcgtaaaac
     3661 taaatcttga tccgcggccg acttctgaat ccaccgctac gttcccgccg tgttgctcca
     3721 cgacttcgcg tacgaaagat aaacccaggc cggtcgattc gtcgtccttc tcctgcccgt
     3781 ctcgaaccac gcgatagaat ttttcccaaa cacgatccaa cgactctgaa ggaataccga
     3841 agccgcggtc ttcaacgcta acgcgaaccg cttctgcctc caatgccgtc gacacagtaa
     3901 ctgtcgtccg ctcagggctg tacttgatgg cgttgttcac taaattcttg acggcttgag
     3961 tgattagacc tttgtcggct gcaaccggtg gaattctttg cgccggctgc tcgataaggc
     4021 gaatgcgttt tttctttgcc agcgattgca ttgacgcaat tgtgtcgcgc acaacctcgt
     4081 ctagtcgaag cggaatcttc aacacttcct gcttgtcttt tcgctctaac tgcgtgacgg
     4141 ctagaaacgt gttaatcatg cgagacatgc gttgcgactc actggcgatg atgccaacaa
     4201 actcgcgtgc ctgttcggga agcgtattgt ccccagtcaa aatttcggca aaaccattaa
     4261 tgctcgtcaa cggagtgcgt agctcgtgcg acattaaaga aatcatgtcg cttttgagct
     4321 tgttgtactc tttcatttga gtcagatctt tgacgtggat aactaatccg atcggatcgc
     4381 gtgagccgcc cagtgcgtct tcactctcgc gcagcagcga gatacgcatc ccgagaatca
     4441 tatttttctg ttcaaacctt agttcacctt cataggcgat tccggtctgc aacactcgcc
     4501 tcacagcgat gatcggttca tcaaagacgt cggtccggaa gtggtcgagt aatccaaaca
     4561 ggtccagact cttagcctgt tcggcttcca aattagcaaa ctttaagagt ggggagttgg
     4621 ttagtgcgat ggtgccgtcg agatatgcga tggcaattcc atcgttgatg tcactcaagg
     4681 cgtttgcctc gcaacgttgt tcagttgtca aagccttcaa tacgtacagg ccttcgagtt
     4741 ttcgctgact gccggtgtgc gagaagaagc tgaacgcctg tgaccgggcg ggattttgca
     4801 agaactcctc acgtttcaaa tttcttgcaa actgggaacc tacagcttcc agaagagctt
     4861 tatcctcatc acagaatcgt cctgctattc tgactagtag tacaccaaca atctcctgct
     4921 ccattcttaa cgggacagcg attttggatg atgtgccatc attcgaaatg tccgccagaa
     4981 gttttccagt agcgatcgcc ttatcacaga gtcgaatgcc gtcgcgccag accgagttac
     5041 gagcttcctg tgcggcgtat cttcgacttt tgattctcgc aatcgtcttg agtgaattag
     5101 actcgagaca tttaaaaacg accgcctcgt ctagtggtag tacggaagtc aataacttta
     5161 atccacttga tagtcgagta gctgcggctc gaccctccgt ttgtccaagc gctttgggtg
     5221 agttcagtag agtccgtgca atctttaggt ccacgcgcca gagtcgaaaa ccttgcagca
     5281 gaattacagt gagaacgccg gtagtaagga gtgaagtcaa caaaagatcg gtggaaaaga
     5341 acctggcaat gagcaagcaa atgaaatttg cgagcagcag agctccagca acaaacggcc
     5401 acgctttgcc aaacgaaaga cggtggatga ccaacccaat cgcaactgag aaaacaatga
     5461 gagccgccag tgcaactttt ggttccggac tcaccaggta accactctca atcgagaaga
     5521 acttgaagaa gatgggagcg agcggaggca ggactccgat gtaccagaga ttgaatgtga
     5581 gcagtgcggc gatgatggaa acgccggccg cagtgatatc tgcgaataag ggcctaagtc
     5641 taatcaaggc gggatatttt gggctcagac taagaggacg attgcccgac gaagggtcag
     5701 agtaggtggc tcgtgactcg agccgccgaa attatacaat gccaagcatt gttaaatcta
     5761 cagtttttcc tgtcgaagaa gtaggttaag agcaaggagg agtagttctt cctgttcttc
     5821 caaaacagtt ctgagatcta gtagtacttt atccgctgaa attctggcaa ttactggcgg
     5881 atttgaagtg cgcaggctgt ttaagatctc ctgagctccg aaagagtcgt gtgaaatagc
     5941 cagcagtgac gttttaatca tcgacgtcgg acctgctcca cctccaattg ccgactctcc
     6001 cgacattagc tcgactctga cagcgggatt ctcgattctt tctattaact tcaacgctcg
     6061 agccttta
//
LOCUS       DASSFP010000342         9231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1425821, whole genome shotgun sequence.
ACCESSION   DASSFP010000342 DASSFP010000000
VERSION     DASSFP010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425821"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1642)
                     /gene="glmM"
                     /locus_tag="VFH91_07595"
     CDS             complement(281..1642)
                     /gene="glmM"
                     /locus_tag="VFH91_07595"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET6862895.1"
                     /translation="MKPLFGTDGIRAEAGQFPLDSATINTIGFSLARHLGEKVERKPQ
                     IVIGRDTRESGSWIEQALTSGAIEGGAECHSAGVITTPGVAYLTRTLPADAGVVISAS
                     HNPFQDNGIKIFSPTGQKMAEAVEKQIEMDVFHSQTDPRSKVQHQPKESSPEFEADLS
                     DRYLKFLTEEIGRGLSLGGLSLVVDCANGASSDLAPRLFQKLGAQVTAINCDPNGRNI
                     NLNCGSLHIEALRQQVIKERVDLGVAFDGDADRSLFVDERGNYVDGDGALWVLAKNLQ
                     DRNQLKDNAVVATVMSNLGLEVALTSRGIKLIRTDVGDKYVLDELLRIKGSLGGEQSG
                     HVIMPHLSLAGDGMITSLCLLRALTDSKESLSALMGGFEHYPQTLVNVRVREKVPFAE
                     LPMVQAAVAEAEERLAQNGRLLLRYSGTERLARVMIEGKNQEEIESYASQIASALRQE
                     IGV"
     gene            complement(1646..2623)
                     /locus_tag="VFH91_07600"
     CDS             complement(1646..2623)
                     /locus_tag="VFH91_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET6862896.1"
                     /translation="MPFQDIDEVSTSEPQRAPSSIEKLLRKIFVEDWNLKLLSLAITL
                     VLWLVVTGQNEPVTAHVNVQLNFIRPQTLEISNDPPKTVDVMLTGSRNKLDDLSSLDL
                     VATVDISDQRAGERVLRLSDKAVISLPQGVKIDGFQPSAVPVRLEPIVDRQLQIEPKL
                     EGKPADGYEVYGITPVKTVVLVRGPASRVYALQTARTESIWLSGNKESFTAQSVAIDI
                     SDPKVDLLDPVIDVDVEIGPKRVEKTFPEIEVTGADGAKISPAVTSVTVAGPPDVIAR
                     LQPNEVKILIDGNGDSLVPRLVGPSGVQHQLLLKSLTPAKFVRTGSIQN"
     gene            complement(2669..3520)
                     /gene="cdaA"
                     /locus_tag="VFH91_07605"
     CDS             complement(2669..3520)
                     /gene="cdaA"
                     /locus_tag="VFH91_07605"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET6862897.1"
                     /translation="MLFLLPYTKYLRRVVEPRSLADIALVFLLVYAVLKLLRGTRAAP
                     MTAGIAAIVFLYWLSVKQDLATLEFVLSNGLLYIGIAIIVLFQSEIRQALITFGNRFR
                     VPFSRVHRGQFGEGVYDEVVLAATTLASTKTGALIVIERNVGLKNVTDGGVQIDAELS
                     YDLLVSIFNPASPLHDGAVVVRRHRVAAASCFLPLTLNPRLSKELGTRHRAAIGVTED
                     TDALAVVVSEETGLISVVQKGEIKRGFDANKLRQAILEALDVLPRKEKEKKISEPDTE
                     TEEIAPI"
     gene            complement(3614..4537)
                     /locus_tag="VFH91_07610"
     CDS             complement(3614..4537)
                     /locus_tag="VFH91_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6862898.1"
                     /translation="MLAVLGALFANGLITVLKLMAAVLTGSSGMMAEALHSVADTTNQ
                     IFLLLGLRFYKRPASAKHPFGYGKERFFWSFIAAIFIFGVGATYAIYEGIEKLRHPHA
                     PTNLEWAYLVLAISFVLESGSIGLAIYQELKEAHHEGLGFFDYIRESKDPTAKTVLFE
                     DSAALLGILIAAAGLLLTEHQVGGSAGAYWDGVASIAIGLVLAVVAFALARTSRGLLL
                     GEAANPKVVQAITRAIESHPNVEKVVELLTMHLAPKEILINAHVKLRPDLVTGDIEST
                     VEDIEKRIKEAEPKVERIFLEAARGDFTATS"
     gene            complement(4795..5943)
                     /locus_tag="VFH91_07615"
     CDS             complement(4795..5943)
                     /locus_tag="VFH91_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6862899.1"
                     /translation="MRLLEFYKRQITVARIYGIPVRIDYRWFIVFGLSVWLIAVNLSR
                     GGMWVGQVKLLPVSITAAWFLAVLSTVGLFLSVFGHELSHALMGRAEGIDIEEIVLHP
                     FGGLARLRNEPESPGAEFRIAVAGPAASFLFAVVGFLLMFLAMIWGYSTVAGVLFLIS
                     AGNLLLAVFNLFPGYPLDGGRVLRALLWRKTGDIQAATRMAGICGMLIAAILIIFGVY
                     MAIAPNFRAYFMGFWSVLVGLFLFDAAYSVVKHARNQLQHIVGEAMSAPFSIEPDLSI
                     SQLIDTILPLHRQVAFPVAHQNRLHGILALEDLKSLPRERWHSTRASEVMRPIAPRFF
                     VEPNATLGFARELMKRNGVGSLAVVGKNGELVGFLQSGEFVRKKQSKS"
     gene            complement(5960..7063)
                     /locus_tag="VFH91_07620"
     CDS             complement(5960..7063)
                     /locus_tag="VFH91_07620"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6862900.1"
                     /translation="MSFIGRTGSFIGLRALRDLVSDSMAIDMGSANTIIAVRGRGVVV
                     DEPSVVSINKVTGEIIAVGIEAHKMQGREARDVAVIAPLVDGVVADFESTRSMLDHFV
                     KKARSGFSHFSRRAIMSVLSEVTQVEQRALLSAAEEARISRVYMIEEGQAAAIGAGVP
                     INDEHASAVVDIGGGTTNVAAIVSGSIIASRAERIGSSDIDTAIMDRLRRHSGLTIGI
                     PSAERIKLELSSAIEPKDPNKKINVRGRDVQTGSPGAVDITAGEVYAVTLPIIRRIGE
                     EVRSMLTELAPEVAGDVYDRGLILTGGGASLDGLPQYLQKELGLAVRVADEPRHAIVR
                     GLSQLFEEPLLLRRVARTEAQPLIENEATAFET"
     gene            complement(7205..8881)
                     /locus_tag="VFH91_07625"
     CDS             complement(7205..8881)
                     /locus_tag="VFH91_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6862901.1"
                     /translation="MAHLKITDASGRQWQYSFLPQATCTIGRAPDNTVVLDDPRASRY
                     HAHIKQLDNGVFTIVDGAVIAGQLRRSANKVFINGEPEFEHQLKTGDRITIGASTLRF
                     EQPAEERTTDVRYDDKPLGHTQLLISANDVMSTVLRSSKEEIVALPSRDKVLESLQRK
                     ANILSALYEMSQTLGSVFDLNAIFAKATDVIFRSTPADRVVALLADAPVAGANPEEVN
                     LTPIAMRARDDKLEAHARKLSIGRTITRKVMKDRVALLSQDAAADEEFAGVDSIVSQG
                     VRSTICAPLMAETRVHGALYADRLDPFAAFKPDDLELISAVAAQTAVAVENARAHERL
                     AREEVARANYSRFLPEYVVKQMLDNPDSFKLGGVNQTITILFADIRGFTRISEHAPPE
                     KIVSLLNRYFSAMTDIIFAHGGTLDKYLGDGLMALFGAPTATPDDASNALNAAVAMQR
                     RLLGINQELEADGLPPIGVGMGLHTGSVIVGYIGSERRSEYTAIGDPVNTSSRLESNA
                     QGGEILISDATAKAAHSRYKLLPREPIMVKNRQQPVVLWEVDWQRASGAF"
BASE COUNT         2292 a         2466 c         2253 g         2220 t
ORIGIN      
        1 cgtgggagtg cgtcgcgccc atccatattc gtggttactg tcctcacccc agcaaccact
       61 tgagacggag gatccaccaa ccgtctctct tgccccacac tatacatcgc aacgcttgtg
      121 ccgtgcaaga gtgattttgg acagcctaac aaccttttag tgtttacaga gcaaaagcga
      181 tcggacgcct cgcagcagag gatcttgcat agttccgacg cccgagcttt gtgtagaatt
      241 tgcgcaaatc cgggtgtaga aatttcacgc cgagtaattt ctagacgcct atttcctgcc
      301 tgagagcgct ggctatctga gaagcataag attcgatctc ttcctggttc ttaccctcga
      361 tcatcacgcg cgccagccgc tcggttcccg aatagcgaag aagcaaacgg ccgttctgag
      421 ccagccgttc ttcagcctcc gcgacagcag cctggaccat cggcaactcg gcaaagggga
      481 ccttttctcg aacacgtaca ttcaccagag tttgaggata atgttcgaag ccgcccatca
      541 acgcggacaa agactcttta gaatcggtga gagctcgtag gagacagagc gaggtgatca
      601 tgccatcacc agccaaactg agatggggca tgatcacgtg accggattgt tcaccaccga
      661 gactgccttt tattcgtagc aactcatcga gcacatattt atcgccaacg tcggttcgaa
      721 ttagcttgat tccgcgactc gttaacgcca cctcaagacc cagattactc atgactgtgg
      781 caacgactgc gttatccttt agctgattac gatcctgaag attcttagcc aacacccaaa
      841 gtgcaccgtc cccatcaacg tagtttccgc gttcgtctac aaagagtgaa cggtccgcat
      901 cgccatcaaa ggcgacaccg agatcgactc gttccttaat cacttgttgc cggagagcct
      961 caatgtgtaa agagccacaa ttgagattta tatttcgtcc attagggtca caattgatgg
     1021 cggtcacttg tgctcccagt ttctgaaaca agcgtggagc cagatccgag ctcgcgccat
     1081 ttgcgcaatc tacgactaaa gataagccac ccaaggacag tccgcggcca atttcttccg
     1141 taaggaattt caaatagcgg tcgctcaaat cggcttcgaa ctctggtgag gattcttttg
     1201 gttgatgctg cactttcgat cgcggatcag tttgcgagtg aaacacgtcc atctcgattt
     1261 gcttctctac agcttcagcc attttctgcc cggttggtga aaaaatctta ataccattgt
     1321 cctgaaatgg attatgagaa gcactgatga ccacacctgc atctgcgggt aaagtcctcg
     1381 taagataggc tactcctggc gtggtgatca ctcccgctga atggcattca gcccctcctt
     1441 caattgctcc agaagtaagc gcctgttcga tccaacttcc cgactcgcgg gtgtcgcggc
     1501 cgatcacgat ttgtggtttt cgttcaacct tctcgcctag atgacgcgcc agagaaaagc
     1561 caattgtatt tattgtcgcc gagtcgagag ggaactgtcc cgcctctgct cggatgccat
     1621 cggtaccgaa tagtggtttc atcaattagt tttggattga gccagtgcgg acaaattttg
     1681 ccggggtcaa ggatttcaaa agaagctgat gctggacacc ggaaggtcct acaagcctgg
     1741 gaacaaggga atcgccattt ccatctataa gtatcttcac ttcatttggt tggagtcgtg
     1801 ctattacgtc tggcgggccg gcaactgtta ccgatgtgac agcggggcta attttggctc
     1861 cgtccgcgcc ggtaacttct atctccggga acgttttctc aacccgtttc ggtccgatct
     1921 caacatcaac atcgatcacg ggatcgagca gatcgacttt cggatcagag atgtcgatag
     1981 ctacactttg ggctgtaaag ctctctttat ttcctgataa ccagatcgat tcagtccgcg
     2041 cagtctgtaa cgcataaacg cggctcgctg gtcctcgaac aaggacgaca gttttaaccg
     2101 gtgtgatgcc atagacttcg taaccatcgg ctggcttgcc ttccagcttg ggttcgatct
     2161 ggagttgccg atcgacgatt ggttctaatc gaaccggaac tgcactgggt tgaaacccat
     2221 ctatttttac gccttgaggt agcgagataa ctgccttatc tgacagtcgc aagactcttt
     2281 caccggcacg ttggtcactg atatcgaccg tagctacgag gtcaagcgag ctcagatcgt
     2341 ccaacttgtt acggctgcca gttaacataa cgtctactgt ctttggcgga tcattactga
     2401 tttcgagtgt ctgaggtctg ataaagttaa gttgcacatt gacgtgagcg gtcacgggtt
     2461 cattttgtcc agtgacaact aaccagagga ctaacgttat ggcgagcgaa agcagcttca
     2521 ggttccagtc ctcaacaaag atcttgcgca ggagtttctc aatggagctt ggagctctct
     2581 gaggttcaga agttgaaact tcgtcgatgt cctggaacgg cataacggtt tactctcaca
     2641 ggcgccggca ggtcggcgca ctccaaaact agatcggggc aatctcttca gtttctgtat
     2701 ccggttccga gatctttttt tccttctctt tcctgggaag cacgtcaaga gcttcaagaa
     2761 tagcctgcct cagtttgttg gcgtcgaatc cacgtttgat ctcacctttc tggaccaccg
     2821 aaatcaggcc tgtctcttcc gacactacca ctgccagcgc atcagtatct tcagttacgc
     2881 caatagcagc acgatgacgt gtaccgagtt cctttgatag tcggggattg agtgttagtg
     2941 gcaagaaaca agatgccgct gcaacgcgat gccgccgtac cacaactgcg ccatcatgaa
     3001 gcgggctggc aggattaaag atgctgacta acaagtcgta actcagttcg gcatcaattt
     3061 gcactccacc atctgtgacg ttctttaatc ctacgttgcg ctcaataaca atcaatgcgc
     3121 cggttttggt agaggcaaga gttgtggctg ccagaacaac ttcgtcgtac acgccctcac
     3181 cgaattgtcc tcggtgaacg cgagagaatg gaacgcgaaa acgattgcca aaggtgatga
     3241 gtgcctggcg aatctcagac tgaaagagga cgattatggc gattccgata tataggagtc
     3301 cgttgctgag cacgaactca agcgttgcca gatcctgttt tactgagagc cagtagagaa
     3361 aaacaatagc cgcgataccg gcggtcattg gtgcggcacg agtgccacga agaagtttta
     3421 gaactgcata cacaagtaaa aagacaagcg cgatatccgc gagacttcgc ggctcaacta
     3481 cgcgtcttaa gtactttgtg taaggcagta gaaacaacat ctctttttat caaatgtatg
     3541 ggcctgcggt tcacgacttt tgttttcata gcgttccaat aattagaacg ccttcaccca
     3601 gattagttat ttcctaagac gtagccgtaa agtctcctcg tgcggcttcc aggaaaattc
     3661 gttcgacctt gggttctgct tctttgatcc gtttttcaat gtcctcgacg gttgactcga
     3721 tatcgccagt caccaggtca ggacgaagtt ttacatgcgc gttaattaag atttcctttg
     3781 gggccagatg catcgttaac aactcgacaa ctttttccac attcggatga ctttcgatgg
     3841 cgcgagtgat tgcctgcacc actttcgggt ttgctgcttc tcctaacaac agcccacgtg
     3901 acgtccgtgc gagcgcaaag gcaactacag ccagcacaag tccaatcgcg atcgaagcaa
     3961 caccatccca gtatgctccg gctgatccgc caacctggtg ttcagttaac aacagaccgg
     4021 cggccgcaat caaaataccc agcaacgcag ctgagtcttc aaagagcact gttttcgcag
     4081 tcggatcttt cgactcgcga atgtagtcaa agaaccccag tccttcatgg tgcgcttcct
     4141 tcaattcctg gtaaatcgcg agtccaattg agcctgactc caggacgaaa gagatagcga
     4201 gaactaaata agcccactcc aggttggttg gcgcgtgggg atgtctaagc ttttcaattc
     4261 cttcgtagat cgcgtacgtc gcgcctacac caaatatgaa aatagctgct atgaaggacc
     4321 agaagaatcg ctcttttcca tatccaaaag gatgcttagc cgacgctgga cgcttataga
     4381 atcgcaaccc caacagcagg aaaatttgat tagtggtatc agcaacggag tgaagcgctt
     4441 cggccatcat tccggatgac ccggttaaga ccgcggccat tagcttgagc acagtaataa
     4501 gtccgttggc aaagagcgcg ccgagtacgg ccaacatgcg gcagaaagtc cttcaacttg
     4561 aaatgacagt tctgactgga ttatcgcgaa aaattgtgag gctggcaatg gtcgtgccgt
     4621 agcaaaatgt gtcgattttg tttggttgtg gccatggaga gcctttcgtc atgagagatg
     4681 gccgaattta gcttccgtgg gcgtaaagtg aatagtcaca atcaaaccag ataattctaa
     4741 aaccttatcc aatttaaact tgaaacgact gaaaccactc acttcgcgac gagatcacga
     4801 ttttgattgc ttcttgcgga caaactcccc actttgcagg aagcctacca actcaccatt
     4861 cttgccaacc actgcgagcg agccgacgcc atttcttttc ataagttcac gagcgaaacc
     4921 caatgtcgca tttggctcaa cgaaaaagcg aggcgcaatt ggtcgcataa cttcactcgc
     4981 acgagtcgaa tgccaacgtt cccgtggcag tgatttgagg tcttcaagag cgagtattcc
     5041 atgtaagcga ttctggtgcg cgacaggaaa ggcgacctgg cgatgtaacg gcaagatagt
     5101 gtcgattagc tgactgatcg acagatcggg ctcgatggaa aatggtgcag acatcgcttc
     5161 gccaacgatg tgctgtaatt gattacgcgc atgcttgact acactgtagg cagcgtcaaa
     5221 gagaaagagt ccaaccagca ctgaccaaaa gcccatgaag tatgcgcgga aattaggagc
     5281 aatggccatg tacacaccaa agattatgag gatggccgcg ataagcattc cgcatattcc
     5341 ggccatgcgc gtagcggcct ggatatcccc agttttacgc caaagcaagg cgcgtagaac
     5401 ccgaccacca tccaggggat aaccgggaaa taaattgaag acggcgagta gcaggtttcc
     5461 agcgctgatc aaaaaaagca ctccagcgac tgtgctgtaa ccccaaatca tcgcgaggaa
     5521 catgagcaga aatccgacaa ctgcaaacag gaaactcgca gctgggccag ccactgcaat
     5581 tctgaactcc gctccaggac tctccggctc gttgcgaaga cgggccaacc cgccaaacgg
     5641 atgcagcacg atttcttcga tatcgattcc ttcagctcgg cccatcagcg catgcgacag
     5701 ctcgtgtcca aaaacggaga ggaatagacc taccgtcgac agaaccgcta agaaccacgc
     5761 ggcagtgatg ctgacgggca gaagctttac ctgccccacc cacattccgc cgcgactcaa
     5821 atttaccgcg atcaaccaaa cactcaatcc gaacacaatg aaccaacggt aatcgatgcg
     5881 taccggtatg ccgtaaattc gggctacagt tatctgacgc ttatagaact ccagcagtct
     5941 catgcgcgga aatcagctct caggtttcaa atgcggtggc ttcattttct atgaggggtt
     6001 gagcttcagt gcgagcaact cggcgcagca gcagtggttc ctcgaacagt tgagagagac
     6061 cacgaactat cgcatgccgt ggctcatcag ccactcttac ggcgagtcct aactctttct
     6121 gcaggtattg gggtagacca tcgagcgatg ctccgccgcc agtgagaatt aagccgcgat
     6181 cgtacacatc accagctact tctggagcca gttccgtgag catggaccgt acttcttcgc
     6241 cgatccgtcg aatgatcgga agagtcaccg catagacctc gccggcagtg atgtcgacag
     6301 cgcctggact tccagtttgt acatcgcggc cgcgtacgtt gatctttttg ttcggatcct
     6361 ttggttcgat tgcagagctg agttcaagtt tgatccgttc agcagaaggt attccgatcg
     6421 tcaacccact gtgacgacgc agacggtcca tgatggctgt gtcgatgtct gagctaccga
     6481 tacgttccgc gcgcgaggct atgattgaac cgctaacgat ggcggccaca tttgtagttc
     6541 cgccaccaat gtctaccact gctgacgcat gctcatcgtt gatcggtaca cctgccccga
     6601 tcgcggcggc ttgtccctcc tcaatcatgt agacccggct gattcgtgct tcttccgcgg
     6661 cgctcaataa ggctcgctgt tcaacctgag tgacttcgga aagcacactc atgattgcgc
     6721 gacgactgaa atgcgagaag ccacttcgtg cttttttgac gaagtgatcg agcatcgatc
     6781 gagtgctttc aaagtcagcg accactccat caaccagggg cgcgatgacg gccacgtcgc
     6841 gtgcttcgcg gccctgcatc ttgtgagctt caattccaac agcaatgatc tcgccagtga
     6901 ctttattaat tgatactact gaaggctcat cgactaccac tccgcgtccg cgtacagcaa
     6961 tgatggtgtt agcggatccc atatcgattg ccatcgaatc tgaaaccaga tctcgcagag
     7021 ctctcagacc aatgaatgat cctgttcggc caataaaaga cataagctaa gtccaaaaga
     7081 tcaggtcagt aaaaactgac tgtagttcaa aaacagagag ggtgaacaag aacggaccac
     7141 agaatttgcc cgctctcatt cacctcccaa acctcccaca acttatgact actgctatga
     7201 aatgtcagaa tgcaccactt gctcgttgcc aatcaacttc ccaaagcact actggttgtt
     7261 gccggttctt aaccatgatt ggctctcgcg gcagcaactt ataccgactg tgagccgcct
     7321 ttgctgtggc atcgctgatc agaatctctc ctccctgcgc gttcgattcg aggcgggaag
     7381 aagtgtttac agggtcgcca atagccgtgt attcggaacg tcgttcagaa cctatgtagc
     7441 cgacaatgac gcttcccgta tgtaggccca tcccaacacc aatcggcggc aacccatcag
     7501 cctctaattc ctgatttata ccgagtaatc ttcgttgcat ggcgacggct gcattcagag
     7561 cattcgaagc atcatcggga gttgcggtcg gagcgccaaa aagtgccatc aacccatcac
     7621 caaggtattt atcaagcgtt ccaccatggg caaaaattat atcggtcatt gccgaaaagt
     7681 agcgattcag caaactgaca atcttctcag gaggcgcatg ctcagaaatt cgagtaaatc
     7741 cacgaatgtc ggcaaaaaga atcgtgatgg tttgattaac gccaccgagc ttgaacgagt
     7801 ctggattgtc gagcatttgc ttaactacgt actcaggaag gaaccggctg tagtttgcgc
     7861 gtgcaacttc ctcacgcgcg agccgttcgt gagctcgggc attttctact gccactgccg
     7921 tctgcgcagc gactgcactg atcaattcca ggtcgtcggg cttaaatgcc gcaaaaggat
     7981 ccagccggtc agcataaaga gctccatgca ctcgcgtctc agccattagt ggtgcgcaga
     8041 ttgtcgaacg aactccctga gacacgatcg agtcaacgcc tgcgaattct tcgtcagcag
     8101 cagcgtcctg cgaaagaagt gccacccgat ctttcataac cttgcgagtg atggtccgac
     8161 cgattgagag ctttcgcgcg tgggcctcaa gcttgtcatc acgagctcgc atggcaatcg
     8221 gtgtcaggtt tacttcttcc gggtttgcgc cggcgacggg agcgtcggcc agcaaggcta
     8281 caacccgatc tgctggagtc gatctgaata tgacgtccgt cgctttcgca aagatggcat
     8341 ttagatcgaa gacggaccct agtgtctggc tcatttcata gagcgcgctg agaatgtttg
     8401 ccttccgttg taacgactcg agaactttgt ctcgtgaggg taaagcaaca atctcctcct
     8461 tcgatgatcg gagcacagtg ctcattacat cgttggctga gatcaggagt tgtgtatgtc
     8521 cgagtggctt atcgtcgtag cgaacgtccg tcgtgcgttc ttcagcaggc tgttcaaacc
     8581 ggagagtgct cgctccaatt gttatccgat cgccagtctt taactggtgc tcgaattcag
     8641 gctcaccgtt tatgaaaacc ttattggcac tgcgtcgcaa ttggccggcg atcaccgcgc
     8701 catcgactat cgtgaagact ccgttgtcta actgtttgat gtgagcgtga taccgagaag
     8761 cccgtggatc atcaaggacc accgtattgt ccggtgcgcg acctatagtg catgtcgctt
     8821 gaggcaaaaa actgtattgc cattgacggc cgctagcgtc ggtgatctta aggtgagcca
     8881 tgcgcgagtg atcctagcag tagcaagtta agctgacaac tttgcagtgc ggcgaaccgg
     8941 cgtcgcttta acacgctacc cgatctcata cattgttcga taaaaagcgg cgccggtcca
     9001 ccgcactcaa aaatttggta actagataca gcctgcgtta tcatgcgcac gctcaactca
     9061 ctcacccaaa ggagcgctca ttatgaggac ctctactcgg ctgggattga tcgccttcac
     9121 ggtgctcaat tgctccttag taatcactga agcctgggcc caaaccaaac caacatcaca
     9181 cagcacgatt agcggtaaag tcaccatcaa aggcaagcct gcgccaggtg t
//
LOCUS       DASSFP010000343         4792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1289065, whole genome shotgun sequence.
ACCESSION   DASSFP010000343 DASSFP010000000
VERSION     DASSFP010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289065"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..2205
                     /locus_tag="VFH91_07630"
     CDS             244..2205
                     /locus_tag="VFH91_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862902.1"
                     /translation="MQRLSLFALIILLISITAGQAFSQDPAPQASPQPSAEDIEKQKA
                     ERERNALRLLDQVIDEAQSLRLVENRVRIQISAADMLWERNQPRARTLFTQAADGVAE
                     LGRTEPTQNNRRGQNIDRSAFQLRQELVLSVARHDAPLAYQLLAATKPPANTQSGPDN
                     RNPRIAFNPEDNLEQVLLGRVAALDPKLAGQNAEQMIDKGQFPRTLPDVINQLQKQDS
                     DAAAKLIDKTLKKIQAANLLTNNDAGNLAVQLLVFGPKMPTASTSSDGSTASAPISGR
                     MIGVLDQSAYTDLLSTVVDAALKAQPQPRNAQVVPQRRGGGPGSAAIAQSTASQPTDA
                     QLEQAGARRLLSSLQATLSNVDQYLPSKASQLRQKLSEFGMGDSSRQNFSQALSALQQ
                     GNANVDTLVQAAASAPPQMQSRIYQQAAYKAIDDGDTDRARQIATSYLQDNARDDVMK
                     RIDARDMVKKAEGAPLDEIRMTLGRLQSDNERIDLLVTVASGLQTTNPKLASQLLDEA
                     KQLAGRRATSYDQFNQQLKVAHAFATVDPARSFEVLEPGITQLNELLAAASVLSGFEI
                     SLFKDGEMSFQGGSGLTGMINRYGQELSFLAKSDFDRAELLTGRFQLAEPRIMTRLAI
                     VQGLLGAQVPQNNFNFQRNIGQTMFSRQD"
     gene            2367..2573
                     /locus_tag="VFH91_07635"
     CDS             2367..2573
                     /locus_tag="VFH91_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862903.1"
                     /translation="MKDSGKLTEKPQTPPKTPYDPLEPDEPVPGIPLPPDTNPQPPAP
                     ITEPGQPEPVKDPTPSGPTRFSAN"
     gene            2651..3217
                     /locus_tag="VFH91_07640"
     CDS             2651..3217
                     /locus_tag="VFH91_07640"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6862904.1"
                     /translation="MGAFGLFLLSALDSSFLFLPFGNDVLLIALVSSERTGLSWILYV
                     LVSAIGSLVGVFLIDLIMRKTGEKGLERFIGPKKLEKLKEKMENKAGVTVFVSTLMPP
                     PFPFTPVVMTASALQCPRSKLFGAVFAGRLIRFTVEALLALYFGRRVIRYINSEWFAY
                     FVYILMAIAFIGSVFSALKWLNQRRQPA"
     gene            3394..3627
                     /locus_tag="VFH91_07645"
     CDS             3394..3627
                     /locus_tag="VFH91_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862905.1"
                     /translation="MPERRGVQATEEIKAEWAKAYKVYLKAPGDRFDKKKDRTARIDF
                     VAQEMKLTRKQAKRRIRNYEAWQRNIKKGIANP"
     gene            complement(3736..>4792)
                     /locus_tag="VFH91_07650"
     CDS             complement(3736..>4792)
                     /locus_tag="VFH91_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6862906.1"
                     /translation="FTIRNERTFQKKMKKTLQIFTLSVLALTFSNQAVSQTKPQDDFK
                     LTPVANGIFAATALPGGLASGNAGFVVGDDGVLIIDTFFTPAAVEELLDAINTQTKQP
                     IKYVVNTHYHLDHTGGNQVFVARGIPILAHDKVIEWQTTRNRKFLPEKDALEKRLDDA
                     QKQLAETPLEQTDKRTQLERQIRRLNAMKSIVLTNPTVTFSTGTVHLYLGAREVVLFT
                     LPGHTGGDVLAFVPDANVLFMGDMGWRKTLPNLVDATVSDWIKSLDTILAQYPSAKFV
                     PGHGEVATAADIKEFRDYLDDLRSRVKKAVSEGLTLDQAKQTLKLPEKYQSFAFQNFA
                     VPNIEDMYKELNGTKAK"
BASE COUNT         1169 a         1173 c         1210 g         1240 t
ORIGIN      
        1 gtcatatcct aatctgccgt cattagatgg catgttcatg gaaatgaagt tggacgtcac
       61 ggagggacgc acccaaagat ttagggtatg gggtcttttt tataggtgaa gtgaaacgtt
      121 tgagcggcaa accgcaccta atcagggtag cgaggcccag acactcgtta tctctcatgc
      181 acctttttgg ccgaaagcca cggtggtaac atctccagaa cttgatttcg aggtaaggta
      241 aaaatgcagc gactgtccct ttttgctctg ataatactgt taatttcgat tacggcaggc
      301 caggcattct ctcaagatcc ggcgccgcaa gcttctccgc aaccctcggc agaagatatt
      361 gagaagcaaa aagctgaacg ggaacgcaac gcgctacgtc tactggacca ggtaattgat
      421 gaagcccagt cgctgcgcct ggtagaaaat cgtgttcgaa tccagatcag tgccgctgac
      481 atgctctggg agcgtaatca gccgcgagct cgcacacttt ttactcaagc tgctgatggc
      541 gtcgcagagt tgggtcgtac tgagccgact caaaataatc gcagaggcca aaatatcgac
      601 cggagtgctt ttcaactgcg acaggagttg gttttgtctg tggcccgaca tgacgcgcct
      661 cttgcctacc agttgctggc cgctactaag cctcctgcca acacgcagtc tggtccagat
      721 aatcgaaatc cgagaatcgc attcaacccg gaagacaatc ttgaacaagt gttgctcgga
      781 cgtgttgctg cgctcgaccc aaagttggct ggccagaacg ccgagcagat gatcgacaag
      841 ggacaattcc cgcgtactct tccggatgtt atcaatcaac tgcaaaagca ggattctgac
      901 gccgcagcga agttaattga taaaactctc aagaagattc aggcggctaa cctcttgacc
      961 aataatgatg ccggcaacct ggccgtgcag ttgttggtct ttggtccaaa gatgccgacc
     1021 gcttcaacct cctctgacgg cagcacagca tctgcaccaa tctccggaag aatgatcggc
     1081 gtcctggatc agtcggccta cacagacttg ttatccacag tggttgacgc agctcttaag
     1141 gcgcaacctc aaccgcggaa tgcccaagtg gttcctcaac ggcgcggcgg tggtcctggt
     1201 tctgcagcga ttgcgcagtc aacggcttcg cagccaaccg acgctcagct cgaacaggct
     1261 ggggcgcgtc gattgttgtc gagcttgcaa gcaactttat ctaacgtgga ccaatatctt
     1321 ccatcaaagg cctctcagtt acgtcagaaa ctttctgagt ttggcatggg ggattcttcg
     1381 cgtcagaact ttagtcaggc tcttagcgcg ctccagcagg gaaatgcaaa cgtagacaca
     1441 ctcgtgcaag ccgcggcatc agctccacca cagatgcagt cgcgcatcta tcaacaagct
     1501 gcttataaag ccatcgacga tggcgatact gatcgagcgc gacagatcgc caccagttat
     1561 ttacaagaca atgcgcgcga tgatgtgatg aagagaatcg acgctcgcga tatggtcaag
     1621 aaagctgaag gtgcgccact tgacgaaata aggatgacgc tcggtcgctt gcagtccgat
     1681 aacgaacgta tagatctgct tgttacagtt gcttctggct tgcaaactac gaatccgaaa
     1741 cttgcgtcgc agttgttgga tgaagcgaaa cagctagccg gtcgtcgcgc caccagctac
     1801 gatcagttta accagcaatt aaaggtggct cacgcctttg ccactgtgga tccagctcgt
     1861 agctttgaag tccttgagcc gggaattact caactgaacg agttactcgc tgccgcgtcc
     1921 gtactgagtg gttttgaaat tagcctattt aaagatggcg agatgtcctt tcaaggcggt
     1981 tcgggtctaa ctggaatgat taatcgctac ggtcaggagt tgtcgtttct agcgaagtcg
     2041 gatttcgatc gggcggagtt gttgacaggt agatttcagc ttgcggagcc gagaatcatg
     2101 acccgtctcg caatcgttca aggcttgctt ggagcccagg tccctcaaaa caatttcaac
     2161 ttccagagaa acatcgggca aacaatgttc tcacgccagg attaatgctc acccttccaa
     2221 cttttattgt tggctggaaa tctccacacg acagatcgag tcttgtagca ctctaattgc
     2281 tatcactctc atggcaacgt ttggtctgca tggttttagg actatgcaga cttttgattc
     2341 gctatcgaat ttttatgaga ctacagatga aagactcggg gaagttgact gagaaaccac
     2401 aaactccacc aaaaacaccg tacgatccac tggagcctga cgaaccggtg ccaggcattc
     2461 cgttgcctcc cgatacaaac ccacagccgc cggctccgat tactgagccc ggtcaaccgg
     2521 aaccggtaaa agatccaacg cctagtgggc caaccaggtt cagtgccaac tgatcacaaa
     2581 cgctttgaag ctgctagtcc atttttttat tcttagcctt tctgtatctg tcttcagatt
     2641 cttcagaagg atgggcgctt ttgggttgtt cctattgagc gcgctcgact cgtcgttcct
     2701 gttcctcccg tttggaaacg acgtcttgtt aatcgcttta gtcagttcag agcggaccgg
     2761 gttgtcatgg atcctgtatg tgttggtctc tgcgattggc agtttggtgg gtgtcttcct
     2821 gattgatctg atcatgagga agactggtga gaaggggctc gagaggttta ttggtccaaa
     2881 gaaattggaa aagttaaaag agaagatgga gaacaaggca ggtgtaactg tttttgtttc
     2941 taccttgatg ccgccaccat ttcctttcac accggttgtg atgacagcgt cagcacttca
     3001 atgtcctcgg tccaaactct tcggagctgt tttcgctggt cgactgatca ggttcacagt
     3061 cgaagcgttg ttggccctgt actttggtcg gcgcgtcatt cgttacataa attcagagtg
     3121 gtttgcctat ttcgtttaca tcttgatggc cattgcattc atcgggagtg tttttagcgc
     3181 attgaaatgg ctcaatcagc ggagacagcc tgcctgatgc atgatttgtt gctggagctt
     3241 gtgaatcttt tgcgcacatc aagaaggcgg tttcaagtta tctttctatt gttttagaag
     3301 actttatgaa tcaaaaaccc gaaaaattcc agattcaaaa ttcgaccgct ttgagtgtag
     3361 aatccgcttg agattctcga aggagcctaa agcatgccag agagacgtgg cgttcaagcc
     3421 actgaagaga ttaaagcgga gtgggccaag gcctacaaag tttacctcaa agcacccggt
     3481 gaccgattcg acaagaagaa agaccgaacg gcccgcatcg acttcgttgc gcaggagatg
     3541 aagcttaccc gcaaacaagc taagcgtcgg atccgaaatt acgaggcttg gcaacgaaac
     3601 atcaaaaaag ggatcgctaa cccttaatcg cgatcccagg taatacggac aataagtaag
     3661 ccattcctca cacggtggtc gtttggagag tgtttaatcc actgtgtgaa tggtggaact
     3721 gtgactatgc gtttatcact tcgcctttgt accattgagt tccttataca tatcctcaat
     3781 gttaggaacc gcgaagtttt gaaacgcaaa cgattgatac ttctccggaa gctttagagt
     3841 ttgtttcgcc tgatcgagtg tcaagccttc ggaaactgct tttttgaccc gactcctaag
     3901 atcgtcgagg tagtctctga attctttaat gtcggcggca gtcgcaacct caccgtgccc
     3961 gggaacaaac ttcgccgatg ggtattgggc caggatcgtg tcgagcgact taatccaatc
     4021 acttactgtt gcgtcaacca ggtttggtaa agtcttgcgc catcccatat cgcccatgaa
     4081 aagaacatta gcgtcaggca cgaaagcgag tacgtctccg cccgtgtgtc cgggcaacgt
     4141 gaatagcacc acctcgcgag caccaagata cagatgtacg gtcccagtgc tgaaagtcac
     4201 agtcgggtta gtcaacacga tcgacttcat cgcgttcaaa cgacgaattt gtctctcgag
     4261 ttgagtccgt ttatccgttt gttccagggg agtttcagcc aactgctttt gcgcgtcgtc
     4321 gagccgcttt tctaatgcgt ctttttctgg caggaacttg cgattcctgg ttgtttgcca
     4381 ctcaatcact ttgtcatggg ccaggattgg aatgccgcgc gcaacaaata cctgattacc
     4441 accggtgtgg tctaaatggt aatgggtatt caccacatat ttgattggct gttttgtttg
     4501 tgtgttgatt gcgtcaagga gttcttcgac ggcagcgggg gtaaaaaatg tatcgatgat
     4561 cagcacaccg tcgtcgccca ctacgaatcc ggcatttccg gaggctagtc caccgggcaa
     4621 cgcggtcgca gcgaatattc cgttggcaac cggcgttagt ttgaagtcgt cctgtggttt
     4681 tgtttgactg acagcctggt tggaaaacgt caatgccagg acggagagtg tgaagatctg
     4741 gagtgttttt ttcatttttt tctggaatgt tctttcattt cttatagtga at
//
LOCUS       DASSFP010000344         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_91354, whole genome shotgun sequence.
ACCESSION   DASSFP010000344 DASSFP010000000
VERSION     DASSFP010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91354"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFH91_07655"
     CDS             <1..989
                     /locus_tag="VFH91_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HET6862907.1"
                     /translation="LLRGIVFGRHGRVFKDAEIKAYLESQSWYKPNAEFSNSMLNDTE
                     RRNLDLIRVAEAGKHDTIQPGDMRYWTQRTITQKKLGKHSGAEWKVLIAEVEAIHGKR
                     FDDEPWLQRYFEERYWYFPSAQYDPKKLTAIERRNVDALTAAQKHQRNVALLPGDMEL
                     FENRSISDKMLQGLSLHELRLLRNEVYARHGRSFRAEWLQQYFFQQPWYAPDENFKDE
                     QLSGSDKTNVETIVRRENEIHQELGTKPITTSLLTGLFLEDAGQMRQEIYARRGKVFK
                     EPWLQKYFASFDWYKPDPNFTEASLTDVEKKNIATIAAYEKRAVTAMSTIEG"
     gene            complement(1048..2136)
                     /gene="tsaD"
                     /locus_tag="VFH91_07660"
     CDS             complement(1048..2136)
                     /gene="tsaD"
                     /locus_tag="VFH91_07660"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET6862908.1"
                     /translation="MQQEMLVLGIETSCDETAAAIVRNGREIVSSVISSQIATHKRFG
                     GVVPELASREHLDKIVPVVNEAFERAQMKPADVDGIAVTVGPGLVGSLLVGVSYAKAM
                     AFALKKPFVGVNHIEGHIYSVAFENPPIEYPAEALIVSGGHTNIFYIPEPGKYKVVAR
                     TRDDAAGEAFDKVAKLLGLGYPGGPIIERLSREGNPKAVPFSIPRMGDGLPDFSFSGL
                     KTAVTKYVRETGLQPVSNDEEPSSAVKDLAASFQHVVVQSLVSTMERIADEYYPKTLI
                     VAGGVACNGKLREECRTIASELGLPVYFPSPHLSTDNAAMIAAAGTVKLQANERGTLD
                     MNADVTLRLQNVDLEEEAARRAGVRYRL"
BASE COUNT          620 a          635 c          630 g          596 t
ORIGIN      
        1 agttattgcg cggaatcgtt ttcgggcgac atggccgtgt tttcaaagat gcggaaatca
       61 aggcgtatct ggagtcacag tcctggtaca aaccaaatgc cgaattcagc aactcaatgc
      121 tgaacgacac tgaacgtcgc aacctcgacc tgattcgcgt cgcagaggct ggtaagcacg
      181 acacgattca gcccggcgat atgcgttatt ggacacagcg caccattacg caaaagaaat
      241 tgggcaagca tagtggagct gaatggaaag tgctgattgc tgaagtcgaa gccatccacg
      301 gtaagcgctt tgacgatgaa ccatggcttc agcgttattt cgaagagcgg tactggtatt
      361 ttcccagtgc gcagtacgat ccgaaaaaac tgacagcgat cgagcgaaga aacgtggatg
      421 ccctcaccgc agcacagaaa catcaacgca acgttgcact tctgccgggc gatatggagt
      481 tgttcgagaa tcgatcgatc tctgacaaga tgcttcaggg tttaagtctt cacgaacttc
      541 gcctgttacg gaatgaggtt tacgctcgtc atggtcgctc gtttcgtgcc gagtggttgc
      601 aacagtactt tttccaacag ccgtggtacg cgcctgatga aaactttaag gacgagcaac
      661 tctccggtag cgacaagacg aacgtcgaaa cgattgttag acgcgagaat gaaattcacc
      721 aggaactggg aactaaaccg attaccactt cattgttgac gggacttttc ctggaagatg
      781 ctggtcagat gcggcaggag atttacgctc gtcgaggtaa ggtttttaag gagccatggc
      841 tgcagaagta ttttgcgagt tttgattggt acaagcctga tccgaatttc accgaggcat
      901 ccctgacgga tgtcgaaaag aagaatatcg ccaccattgc ggcttatgaa aaaagggccg
      961 tgacggcgat gagtacgatt gagggttgat aaagcccgac ctaagtctcg cgtgacgaga
     1021 ctacgaaaca aaaacgaaaa cgaaaagcta aagccggtac ctcactcctg ctcgtctggc
     1081 cgcttcctct tcaagatcaa cattctgaag gcgcagtgtc acatcggcgt tcatgtcgag
     1141 cgttccgcgt tcgtttgcct gtagcttgac tgtaccagct gcggcaatca tcgcggcgtt
     1201 gtctgtggaa agatgtggcg acgggaaata gacaggtaag cccagttctg aagcaattgt
     1261 gcgacactcc tctcgtaact tcccattaca ggcgactccg ccggcaacta tcaaggtctt
     1321 tggataatat tcgtcggcaa tccgctccat cgtgctcacc agcgactgga ccacgacatg
     1381 ttggaaactc gcagcgagat cttttacggc agacgatggc tcttcatcat tacttactgg
     1441 ctggagacct gtctcgcgca catactttgt gacggctgtt ttaagtccgc taaaactaaa
     1501 gtcgggcaaa ccgtcgccca ttcgcggtat agaaaatgga actgcttttg gattcccctc
     1561 gcgggacagt cgttcgataa tgggaccacc cggataacca agcccgagta gtttcgccac
     1621 cttgtcaaaa gcttcacccg ccgcatcatc acgggttctt gcaacaacct tatattttcc
     1681 aggctccgga atatagaaga tgtttgtatg gcctcctgag acgatcaaag cctcggccgg
     1741 atattcgatt ggcggatttt caaatgctac tgaataaata tggccctcga tatggtttac
     1801 accgacaaaa ggttttttca acgcaaaggc catcgctttt gcgtatgaaa cgccaaccag
     1861 cagcgagccc acgaggcctg gaccaacagt aacagctatt ccatcgacat cggccggctt
     1921 catttgagct cgctcaaagg cttcgttgac aacgggtacg attttgtcca ggtgttctct
     1981 cgaggctagt tcaggtacca cacctccgaa gcgtttgtgc gtagcaatct gagacgaaat
     2041 tacagaggac acgatctctc gaccgtttcg tacgattgcc gctgccgttt cgtcacaaga
     2101 tgtttcaatt cccagtacaa gcatttcctg ttgcatgcgt aggattttac aagacgacca
     2161 gacgattgtc gctgcgcccc caaataggtt gttttctctg cgtaactctg cggtcctccg
     2221 cgtaactctg agtttagtcc tgaccttttt ataaagagtt caacgcagcg ttacgcagag
     2281 agccgacaca agaatgttgc tgctgagttc tttaactcga caatgtcagc gggcttcacc
     2341 ccggtagggg tgaaatgttt atagcccacc ggatgcctga cctccggcgc cgttcggagg
     2401 agcgggaatt aactagccct ataacggcca gacgcctccc gctcctccga atggcgcgct
     2461 ggcacggtgc ttgttctcgg c
//
LOCUS       DASSFP010000345         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1426138, whole genome shotgun sequence.
ACCESSION   DASSFP010000345 DASSFP010000000
VERSION     DASSFP010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426138"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFH91_07665"
     CDS             complement(<1..204)
                     /locus_tag="VFH91_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862909.1"
                     /translation="MAQPIPLDVSPRNPQRELELKLSRAPEKYAEATLAGYELLQALH
                     DEGVLEALRSTVGGGAKILEQAVT"
     gene            complement(206..>2510)
                     /gene="fdhF"
                     /locus_tag="VFH91_07670"
     CDS             complement(206..>2510)
                     /gene="fdhF"
                     /locus_tag="VFH91_07670"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET6862910.1"
                     /translation="DGMIDLVKGVEPETGYGTIMRVSEAEEGMRESRIRRTKTVCTYC
                     GVGCSFDVWTKDRHILKVEPLDGPANGVSTCIKGKFAWDHINSGDRLTSPLIREGDKF
                     REASWDEALGLVGRRLGEIKAQYGPDSIGVIASSKCTNEESYLMQKFARAVIGTNNID
                     NCSRYCQSPATTGLFRTVGYGGDSGSITDIAQADLVVIIGSNTAESHPVLATRVKRAH
                     KLHGQKLIVADLREHEMARRADVFMHTAPATDLIWLSAVSRYLIDNGLVKKDFINRWV
                     NGFDNYRATLEPFTMEFAAQRCGVPVETLVNVAHMIAKAERVCILWAMGVTQHSQGSD
                     TSTAISNLLLLTGNYMRPGTGAYPLRGHNNVQGASDQGSMPNNLPGYQSVDDPEVRGR
                     FEKGWNVKLPSTKGLDNHEMVDAIQAGKLKALYLGGEEMSIVDSNANYVCDAFSKLDF
                     MVVQEVFFGVTCQFADVIFPAAPSLEKEGTFTSTERRFQRLYQVFEPLEGSRPDWRII
                     QDVANQLGAGWNYHHPSEVMDEIASLTPMFAGVTYDRLEGYRSLQWPVAADGTDQPLL
                     YTKEFFFPDGKARLFPLKWVEPRDQPDSEYDLHLNNGRLLEHFHEGNLTYRTEGIREK
                     TPDTFVEVSPELAQERGIQSGRFVQLTSRYGKVRVRAVVTDRVRGKQMYMPMNSTESP
                     VNRLTSSYTDSVTHTPAYKEVSVKMTVLPEIGDSPLPRTNSRWGTPTPQRGVEVDRKW
                     NRSDYHLPGPKLFQIQPRQATNAEVRK"
BASE COUNT          576 a          679 c          608 g          647 t
ORIGIN      
        1 agtcaccgct tgttcgagta tctttgcgcc gccacccact gttgagcgta gtgcttcaag
       61 caccccctcg tcatgtaacg cttgtagcaa ctcgtaaccg gccagtgttg cctcggcata
      121 tttttcggga gctcgagaaa gcttcagctc cagttcacgt tgggggttac gcggtgatac
      181 atctaaggga attggttggg ccatcttact ttctcacctc tgcattggtc gcctgtcggg
      241 gctgaatctg aaatagtttt ggaccaggaa gatgataatc agatcggttc cacttccggt
      301 ccacttcaac tccgcgctgg ggtgtcggcg taccccagcg ggagtttgtc cggggaagcg
      361 ggctgtcgcc gatttccggt agcacagtca tctttaccga gacctcttta tacgcaggtg
      421 tgtgggttac ggaatccgtg taactactgg tcagccgatt caccggactc tcagttgaat
      481 tcattggcat gtacatttgt tttccacgca cacgatctgt aacgaccgct cgaacgcgaa
      541 ctttaccgta gcgtgaagtt aattgaacaa aacgtcctga ttggattccc ctctcttgcg
      601 ctagttccgg agagacttcg acaaacgtgt caggggtctt ttcgcgaatt ccttcagttc
      661 tgtaagtgag attgccttcg tgaaaatgct caagcaggcg accattgttc aaatgtagat
      721 cgtattcgct atccggttgg tctcgcggtt caacccattt aagtggaaac agtctcgcct
      781 ttccatccgg aaagaaaaat tctttggtgt aaagcaacgg ttgatcggta ccatccgcag
      841 ccacaggcca ttgcagagag cgataaccct cgagccggtc atatgtgaca ccggcaaaca
      901 tcggagtcag agacgctatt tcatccatga cttcagacgg atgatgataa ttccaaccgg
      961 cacctagctg gttcgcgacg tcctggataa ttctccagtc aggcctgctc ccctctaacg
     1021 gttcaaacac ctgatagagt ctttgaaagc gacgttcagt gctggtaaat gtcccttctt
     1081 tttccaggct gggagcggcg ggaaaaataa cgtcggcgaa ctggcaggtc actccgaaaa
     1141 agacttcctg gaccaccatg aagtcgagtt tcgaaaaggc atcgcaaacg tagttcgcgt
     1201 ttgagtcaac gatactcatc tcctcaccgc caaggtagag ggccttcaac ttacctgctt
     1261 gaattgcatc gaccatttcg tgattatcca agcccttggt tgaaggcaac ttgacgttcc
     1321 agcctttttc aaaccgtcct cgtacctctg gatcatcaac tgattgataa ccagggagat
     1381 tgtttggcat cgatccctga tcactagctc cttgcacatt gttgtgtcca cgcaatggat
     1441 aggcaccagt gccgggtctc atgtagtttc cggtcagcaa caacaggttc gagattgctg
     1501 tcgaagtgtc tgagccttga ctatgttgcg tgactcccat ggcccagaga atacagaccc
     1561 gttctgcctt ggcgatcatg tgggcgacat tcaccagagt ctctaccgga actccacacc
     1621 gctgtgctgc aaattccatc gtgaaaggct cgagcgttgc gcgatagtta tcgaaaccat
     1681 tgacccaccg attgatgaag tctttcttca cgagtccgtt atcgatcagg taacgactga
     1741 ctgcggataa ccagataaga tccgttgccg gtgccgtgtg cataaagaca tcggcgcggc
     1801 gagccatttc gtgttcacgc aaatccgcga caatcaattt ttgaccgtga agcttgtggg
     1861 cccgcttgac cctggtcgcc agtactggat gactctcagc ggtattgctg ccgatgatca
     1921 ccaccaggtc agcttgcgca atgtcggtga ttgagcctga atcgcctccg taaccaacgg
     1981 tccgaaacaa tccggtggtt gccggtgatt ggcaatagcg agaacagtta tcgatattgt
     2041 ttgtgccgat taccgcacgt gcaaacttct gcatcaggta gctctcttcg ttggtacatt
     2101 tcgaagaagc aatcaccccg atcgagtcag gaccatactg ggccttgatt tcaccaagtc
     2161 gacggccgac aagtcccagc gcttcgtccc aactcgcttc tctgaatttg tcgccctcgc
     2221 gaattaaagg agaggtgagt cgatcaccgc tattaatgtg atcccaggca aatttcccct
     2281 ttatacaagt cgaaacacca ttggctggtc catccagtgg ttcaactttc aagatgtggc
     2341 gatccttggt ccacacatca aaactgcaac cgactccgca ataagtgcag acggtcttag
     2401 tgcgccggat acgagattcg cgcatccctt cctcggcttc ggacacacgc ataatcgttc
     2461 cataaccagt ttctggttcc actcccttta ccaaatcgat catgccgtca
//
LOCUS       DASSFP010000346         5012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2181460, whole genome shotgun sequence.
ACCESSION   DASSFP010000346 DASSFP010000000
VERSION     DASSFP010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181460"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFH91_07675"
     CDS             complement(<1..1304)
                     /locus_tag="VFH91_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862911.1"
                     /translation="MSVEQPNNPEVQPETPKPSIARRWRYFSRRHAILAGIIVSAGGL
                     FLILLVLFLYRFGYVDRYVASQVKNTFANYGIRAEIRDFHATFPPQTVEMVGVELFDA
                     QSGEKLGKIDRLLATIRIEDLYALNLQRNINLRDLKIEGFEAWVNFDEQGRSNFRNIH
                     IPPPEPNKRILFAYSTAHVEVTNGVVHYGDARHEISGEARNLQATVQPDDPNSPADSW
                     MNTVAFSMTDSTFTYDGHPVDKIDVQAHGRVNQTRVEIQELVLKSPVAEARLQGTMDD
                     WRALRYQMNITSSVDLTQISETFQTKAALRGAGNFSGTVTGEGDKFQVQGSIQSDALA
                     ADNMRLQGLNVTATGSGQGKSYEINGKAVAKLLSAGDFQIDSLQLVGNVMGTGTDFRW
                     VGELRAVAERSYGTTLSGLILRDARAEMNKGVLTASSSQFAA"
     gene            complement(1507..2706)
                     /gene="ribB"
                     /locus_tag="VFH91_07680"
     CDS             complement(1507..2706)
                     /gene="ribB"
                     /locus_tag="VFH91_07680"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET6862912.1"
                     /translation="MTLAAIDEAVAEIRNGRMIIIIDDEDRENEGDLVCAAEKVTPEI
                     INFMARHARGLICLPLTEERCDELHLTTQVADNTSYLGTAFTISIDARKGITTGISAA
                     DRATTILAAVDSHSRPQDLARPGHIFPLRAKNGGVLVRPGQTEASVDIARIAGLYPAG
                     VICEIMNEDGTMARLAELKEFAAKHDLKMISVADLVRYRISKEKLVRRVVESDLPTVY
                     GRFRAVAYENIINGDVHLAMVMGDVTTDEPVLVRVHTENLTCDMFGSLIDDTGFQLHT
                     ALEKIAAEARGVVLYLRQREHSLDLVNQLRTYALMQERQISKSEASLETGYGIDRDYG
                     VGAQILHDLGLREILLLSNHPPKVAALEGFELSVVGNVPLGEPAAFNDQPTANDSRRA
                     IKKEQRT"
     gene            complement(2879..3472)
                     /locus_tag="VFH91_07685"
     CDS             complement(2879..3472)
                     /locus_tag="VFH91_07685"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET6862913.1"
                     /translation="MFTGIIEELGHVSLIEERGENARIVISASVVTLGTNHGDSIAVN
                     GVCLTALDITDGSFAADVSRETLRRSTLGRLKQGAPVNLERAVTPATRLGGHIVQGHV
                     DGRGKFLGVDSHGESWTLRISYPEELGRYLVFKGSVSVEGISLTIANLTTEYFEIAII
                     PKTWEMTNLSTLAPGDDVNLEVDVIAKYVERLLKPAP"
     gene            complement(3702..4373)
                     /gene="cmk"
                     /locus_tag="VFH91_07690"
     CDS             complement(3702..4373)
                     /gene="cmk"
                     /locus_tag="VFH91_07690"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET6862914.1"
                     /translation="MPLIIAIDGPSGSGKSTLGRMLARALNLIYVDTGSMYRAVALAV
                     MNASISPSDAAAVTRLAETLDIDLAGDPDSLKVFLNGSDVTDLIRSEEVTRMSSIVSA
                     IPGVRRAMVALQRQIGLKGAVLNGRDIGTVVFPDAHYKFFLTASAEERATRRYKEDVT
                     KKSDVSLAETLADMTERDLRDSTRADSPLKIANDAIVLDSSGMSIDEVFNFMMATVTR
                     EKFGV"
     gene            complement(4472..>5012)
                     /locus_tag="VFH91_07695"
     CDS             complement(4472..>5012)
                     /locus_tag="VFH91_07695"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862915.1"
                     /translation="LAEHIRKGIQPCPDDDLAGVMYEYMLERSLDAGYEHYEISNLAR
                     PGFASRHNTKYWTGAPYVGFGCSAHSYDGRKLRWSNERDVRNYVNLVEQGSSPIVDQQ
                     LLSDSAVRAEAVFLGLRVMSGINLADYRDLFGVDLLEQYNEDLNRFKDAGLLELEGEL
                     LKLTRAGALLSNEVFATFV"
BASE COUNT         1231 a         1323 c         1201 g         1257 t
ORIGIN      
        1 tctgctgcaa actgagacga tgaagcagtt aatacacctt tattcatctc agcccttgca
       61 tctctcagaa tcaaccccga cagcgtagtt ccgtaactgc gctcagccac cgcgcgcagc
      121 tcgccgaccc aacggaaatc tgtgccggtt cccatcacat tgccgactag ttgcaacgag
      181 tcgatctgaa aatcgccggc gctcagcagt ttggccacgg ccttaccatt aatttcgtaa
      241 gacttccctt gtcccgatcc ggtggcggta acatttaatc cttgcaaccg catgttatct
      301 gccgccaatg catcagactg aatagaacct tgaacctgga atttgtcgcc ttctccggta
      361 acagtaccgg aaaaatttcc cgctccgcgt aaagccgctt tggtttgaaa agtttcgctg
      421 atttgcgtga ggtctacaga tgaagtgatg ttcatctgat atctgagtgc gcgccaatcg
      481 tccattgttc cctgcagccg agcctctgcg acaggtgact tgagaactaa ctcctgaatt
      541 tcaacgcgtg tttggttaac tcgcccatga gcttgaacat cgatcttatc taccggatga
      601 ccatcataag taaacgtcga gtcggtcatg gagaatgcaa cagtattcat ccaactgtcg
      661 gctggcgaat tgggatcatc tggttgaacg gtagcctgaa gatttcgagc ttcaccggag
      721 atttcgtggc gggcgtcgcc gtaatggacc acgccattgg taacttctac gtgtgctgta
      781 gagtaggcga atagaattct cttatttggc tcaggtggcg gaatatggat gttgcgaaaa
      841 tttgaccgtc cttgttcgtc aaagttaacc caggcctcga atccctcaat cttgagatct
      901 cttaagttga tattcctctg aagattgagg gcgtatagat cttcaattct gatagtcgct
      961 aacaatcgat ctatttttcc gagtttctcg ccagactgag cgtcgaagag ctcgacacct
     1021 accatttcga cagtttgcgg tggaaaagtc gcatgaaaat ctctgatctc ggctctgata
     1081 ccatagtttg caaaagtgtt cttgacctgg ctcgctacgt accggtccac gtaaccgaag
     1141 cgatataaaa ataggaccag gagaatcagg aacagacctc cggcggaaac aattattccg
     1201 gcaagaatcg cgtgccggcg actgaaatag cgccaacgcc ttgcgattga gggctttgga
     1261 gtttcgggtt ggacttccgg attgtttggt tgctctacac tcataacctt aggccgtcgc
     1321 gcggtccatg gggaaatcga attctagagg gagagtgcga aacttgaaag tgcttttatc
     1381 ccgtcgtttt gctttacacc tgagtgtagc ccatgttttt ccggagggtg tcttagattt
     1441 tgtcacgcac cttcagcgca tgcaaaactc aaacctgggc caccatgcct ccgatgactg
     1501 aagcattcaa gtgcgttgct ctttcttaat cgcacgtcgg gaatcattgg ctgttggctg
     1561 atcattgaac gcagccggct cgcccagcgg gacatttccc acaactgaga gttcaaaacc
     1621 ctcgagagcg gcaactttgg ggggatgatt ggatagaaga agtatttcac gtaacccgag
     1681 gtcgtgcaga atttgtgcgc cgactccgta gtctcgatcg ataccataac cggtctcaag
     1741 actggcttca gatttactga tctgccgttc ctgcatcaac gcgtaagtac gtaactgatt
     1801 gacaagatcc agactatgct ctcgctgtcg caagtaaagg acgactccgc gagcttcggc
     1861 agcaattttc tctagagcag tatggagttg aaagccggta tcatcgatca aagagccaaa
     1921 catatcacag gtaagattct ccgtatggac tcgcaccaga accggctcgt cagtcgtaac
     1981 gtcgcccatc accatcgcga gatggacatc accattgatg atattttcat acgccacggc
     2041 gcgaaatcgt ccatagacag tcggcaagtc actttcgacc acacgacgga ccaacttctc
     2101 cttactgatg cgatacctga cgaggtcagc gacactgatc atctttagat cgtgtttagc
     2161 cgcgaactcc ttcagctctg caagtctggc catagttccg tcttcgttca taatctcgca
     2221 gatcacaccg gccggatata agcctgcgat tcgggcgatg tcgacgctag cttcggtttg
     2281 acccggacga acgagcacgc caccgttttt cgctctcagc ggaaagatat gtccgggccg
     2341 cgccagatcc tgcggacgag agtgtgaatc aacagcggca aggatggtag ttgcgcgatc
     2401 tgcagcagaa attccagttg ttatgccttt tcgcgcatcg atcgaaattg taaatgctgt
     2461 ccctaagtat gaggtgttgt cggctacctg agtcgtcagg tgtagctcat cgcagcgctc
     2521 ttcggttaat gggaggcaga tcagaccgcg agcgtgccgg gccatgaaat tgatgatttc
     2581 gggcgtaacc ttttcagccg cgcacacgag atcgccttca ttttcgcgat cttcgtcatc
     2641 aataattatg atcatccggc cattgcgaat ctcagcaaca gcctcatcga tagcagcgag
     2701 cgtcatcagg atttgagcaa gcctttcggg acgtttaaag cagcccacgc aacagttaac
     2761 aaaatggcaa gttatgttaa ccgaaagccc gctatttgag aaaacccgga ataaggatca
     2821 aatcgtcaat cgataattgt taattaattt ctggccggga agtgccggta ctccaaagct
     2881 agggagctgg tttaagtagc ctttcgacgt acttggcgat cacgtcgact tccaaattca
     2941 catcgtcacc cggagccaga gtcgacaaat tggtcatctc ccaggttttt ggaataatcg
     3001 ctatctcgaa atattcagtg gtcagattag cgatcgtcag actaattccc tcaactgaaa
     3061 ctgaaccttt aaacacaaga tatcgcccaa gctcttcggg atagctgatt ctcaatgtcc
     3121 aggattctcc gtgactgtcc actccgagaa actttccacg gccgtcgacg tgtccttgaa
     3181 caatatggcc accgagacga gtagctggcg tgactgctct ttccagatta acgggcgctc
     3241 cctgtttgag acgcccgagg gttgatcgcc gcaacgtctc gcgcgaaacg tcagcagcga
     3301 aagagccgtc cgtaatatcg agcgcggtga gacaaacacc gtttaccgcg attgaatcgc
     3361 catggtttgt accaagtgtc acgacacttg ctgagattac aatgcgcgcg ttctcgccgc
     3421 gctcttcaat aaggctaacg tggcccaatt cttcgatgat gccggtgaac atgtggattt
     3481 gtgttttgta ctttgtgctt tgagtttacc ttgtcatgag gcgcaacagc caaacgataa
     3541 gagcaacgag taaacctaaa ggtatcggaa gtacgctcag ccacagcatc cagatggctt
     3601 cgcgtttgaa tatggatttg ccagcattca tgccgactct tgcctttgcg agttgtcgtt
     3661 gatctctgac tttgcgcttt gagatctgag atccccaaag ttcacacccc aaatttctct
     3721 ctagtgacgg tggccatcat aaaattaaag acctcatcaa tcgacatccc tgatgaatct
     3781 aaaacgatcg catcgttagc aatcttgagt ggcgaatccg cccgggtcga atcacgcaaa
     3841 tcgcgctcag tcatgtccgc aagagtttct gctaacgaca cgtcggattt tttcgtgaca
     3901 tcctccttat aacgccgggt cgcccgttcc tcagcagaag ccgttagaaa aaacttatag
     3961 tgggcatccg ggaacacgac tgtcccgatg tctcgaccat ttagaacggc gcctttgagt
     4021 ccaatctgac gttgcaaagc caccatcgct cgtctaactc caggaatcgc ggatacgatt
     4081 gacgacatcc tggtgacttc ttcgcttcgg atgagatccg tcacatcact gccgttcagg
     4141 aaaaccttaa gtgagtctgg atcgcccgca aggtcaatat ccagagtttc ggctaaacgc
     4201 gtcactgctg ctgcgtcact cgggctgatc gacgcgttca ttaccgccaa agcaacggct
     4261 cgatacatcg aacccgtgtc gacgtaaatt agatttaacg cgcgtgccag catgcggccc
     4321 agcgtcgatt tacctgaacc agatggtcca tctatcgcaa ttattagagg cactttatat
     4381 tctgttcgtg gttcgtcgtc cgttgtccgt tgccgcagtt agcaaaagat aagtaaggcg
     4441 cgccctacca aacactaaac aacggaccac gctagacgaa tgttgcgaaa acttcattcg
     4501 agagtaatgc tccagcgcga gtcagcttca gtaactcgcc ttcgagttcc aatagacctg
     4561 cgtccttaaa cctgttcaga tcttcgttgt actgttcaag cagatcgacg ccaaagagat
     4621 cccgatagtc tgcaaggtta attccgctca taactcgcaa ccccagaaag actgcttcgg
     4681 cacgaactgc ggaatcgctc aacagttgct ggtcaactat tggtgacgaa ccttgctcaa
     4741 ccagattcac ataattgcgg acgtccctct cgttggacca gcgcagcttc cggccatcat
     4801 acgagtgagc agaacagcca aaaccaacat atggtgcgcc ggtccaatac ttcgtgttgt
     4861 ggcgagatgc gaagccgggt cgcgcaagat ttgaaatctc gtagtgttcg tacccggcgt
     4921 ccagagaacg ctccaacatg tattcgtaca tcacaccggc cagatcatcg tcgggacaag
     4981 gctgaattcc ctttcgtatg tgttcggcca gc
//
LOCUS       DASSFP010000347         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1129332, whole genome shotgun sequence.
ACCESSION   DASSFP010000347 DASSFP010000000
VERSION     DASSFP010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129332"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFH91_07700"
     CDS             complement(<1..807)
                     /locus_tag="VFH91_07700"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET6862916.1"
                     /translation="MPDITLARSSGILLHPTSFPGPFGIGDLGKTAYQFADFLVASGQ
                     SLWQVLPLGPTSYGDSPYACYSAFAGNTLLISPERLVDEDLLAADEVAPLITDSDERI
                     DFVRVHHHKEGILRDAFSRFKASSQSQLKLAFEKFCVDEQDWLDDYGLFRALKEVHSD
                     RPWNEWHPALVKRSSVEIGQARHAFAHRIEAHKFYQFLFFRQWFELKSYCNKLGIKLI
                     GDIPIFVAQDSSDVWTNPDQFKLDGHGIPTVVAGVPPDYFSSTGQLWGNPI"
     gene            complement(852..2954)
                     /locus_tag="VFH91_07705"
     CDS             complement(852..2954)
                     /locus_tag="VFH91_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6862917.1"
                     /translation="MQGLENYVGQTLDDKYRLEQLLGQGGMGAVYLATHLGTDRYVAL
                     KLIAPRFMRNAEFVERFKREARAAGRLRHPNVVDVTDFGFSRVKEGQVAYLVMEYLDG
                     CTLGDILSEENHLPLEWVVDILEQVCSAVHEAHQLGIIHRDLKPDNIWLEPNRLGSYR
                     VKVLDFGIAKLGESAPATSEDDPVSTIIDDVSRIVSLSDAAEGDEEVALEAKTLLNPS
                     ESDHQNQHRQVNTERAALGTALKKESTADAESTKLFSAGTVTEKPRALTQEFASAHPE
                     DADGTLLFEQGTHSNRQTAGASTHVEGAALTRVGAIMGTPLYMSPEQCGGGQVDIRSD
                     IYSLGVIAYQMLAGEPPFSGNTSTVMRAHREETARHLHDYEVKIPKRVAGVVMAALSK
                     DPNQRPQTAFAFASSLRGQAEGISALYRRAFSLYSEYFPKFVKLSIIAHLPVIAVAAL
                     MICVLVADKMFDPVGKVQKGVMISVTVLVGLLQIAAYFVAAGVISGVTAIIVTQLSAA
                     PLRPVKLRVAFAVLRRRWKPFLKTMVRITFRIIFGYILFFIPGIVMSIRYALYAPVVL
                     IEGLEKKAAMRRARELASRSWRTVIIVSILQILIPICVSLLLGRIRVGVSQDLKQANL
                     GRQISQQILGLANIFVVPLMSIVPALLYLKMRQLGGETLSAAMAQMEEVDVGRRLWQQ
                     RMRTRLSLYTPSDQKTTG"
BASE COUNT          868 a          839 c          788 g          827 t
ORIGIN      
        1 aattgggttt ccccatagtt ggccagtact gctgaaataa tctggaggga ctccggcaac
       61 gaccgtcggg attccatgtc catcaagttt gaattgatcg gggttggtcc aaacatcaga
      121 tgaatcctga gccacgaata tcgggatatc gccgatcagc ttgatcccta atttgttgca
      181 ataggatttc agttcaaacc actgccgaaa aaatagaaac tggtagaact tatgtgcttc
      241 gattcggtgc gcaaaggcgt gccgcgcctg accgatttcc acggagctgc gctttaccaa
      301 agccgggtgc cattcattcc atggtctatc ggagtgaact tctttcaacg cacgaaacag
      361 accgtaatcg tccaaccaat cctgctcgtc gacacagaac ttttcaaatg ctagtttcaa
      421 ttgcgattga ctggacgctt tgaaacgtga gaacgcatcc cgcaatattc cttctttgtg
      481 gtgatgcaca cgaacaaaat ctatgcgctc gtccgagtcg gtaattaacg gcgctacttc
      541 atcggcggcc agaagatctt catcaacgag tcgttcggga ctaatcagca gagtgttgcc
      601 ggcgaaagct gagtagcaag cgtagggcga atctccatag ctggttggac caagtggtag
      661 aacctgccaa aggctctgtc ctgaagccac gagaaaatcc gcaaattgat aagcagtctt
      721 tccaagatca ccgataccga agggacccgg aaaagacgtt ggatgaagta ggataccgct
      781 ggatctggca agggtaatgt caggcatttt agactgcaac aagaatgagt ttatcggtgt
      841 cttaatcgaa gctaaccagt ggttttctgg tcactcggag tgtaaagact cagacgcgtt
      901 cgcattcgct gttgccagag ccggcggccg acatcaacct cttccatctg agccattgcc
      961 gcactcaacg tctcgccgcc gagttgtcgc atcttcaaat agaggagcgc aggtacgatc
     1021 gacatcaaag gcactacgaa aatattcgcc aagcccaaaa tctgttgtga tatttgtcgt
     1081 cccaggttcg cctgtttcaa gtcctgactg actcctactc gaattcgacc caacaagaga
     1141 ctgacgcaaa ttggaatgag aatctgcaag atcgaaacga tgataacagt tcgccaggaa
     1201 cgcgaagcaa gctctcttgc tcgacgcatt gcagctttct tctcaagccc ttcgattaga
     1261 acgaccggcg cgtagagcgc ataacgaatc gacattacga tgcctggtat aaagaacaga
     1321 atgtagccaa agataattcg aaaggtgatt cggaccatcg ttttcaggaa cggcttccat
     1381 cgtcttctta gaactgcaaa agcaactcgc agtttaactg gccgcagcgg cgcggcagat
     1441 agttgcgtaa cgatgatggc agtaacacct gagatcacac ccgccgcaac gaagtaagcg
     1501 gcaatttgta aaagaccaac aagcactgtt actgaaatca ttactccttt ttgcacctta
     1561 ccgactggat caaacatctt gtcggctacc aaaacgcaaa tcatcagagc cgcaaccgca
     1621 atcaccggga gatgcgcgat aatcgacagt tttacgaatt ttggaaagta ttcactgtaa
     1681 agagaaaatg ctcgccgata cagagcgcta attccttccg cctgtcctcg caaggaactg
     1741 gcaaaggcaa aagctgtttg cggtctctgg tttggatctt tcgagagcgc agccattaca
     1801 actccagcga cacgcttcgg aatttttact tcgtaatcgt gcagatgccg agctgtttcc
     1861 tcgcgatggg ctcgcattac cgttgatgtg ttgccgctaa acggtggttc accggccaac
     1921 atctggtaag cgattactcc gaggctatag atatcagagc gtatatctac ctgaccgccg
     1981 ccgcattgtt ctggcgacat atacaaagga gtacccatga tcgcgccgac gcgagttaaa
     2041 gcagcgcctt cgacgtgagt tgatgcgccg gcggtttgtc tgttggagtg ggttccctgc
     2101 tcgaataaca gagttccatc agcgtcttcc ggatgagctg atgcaaattc ttgagttaat
     2161 gcgcgcggtt tttctgtaac ggtccccgca gaaaaaagtt ttgtgctctc agcatcagcg
     2221 gtcgactcct ttttaagcgc cgttcccaat gcagctcgct cggtattgac ttgacggtgt
     2281 tgattttgat ggtcgctttc ggacggattc aataacgtct ttgcttcaag cgcgacttct
     2341 tcatcgccct cggctgcgtc cgagagacta acgatacgtg aaacatcgtc gatgatagta
     2401 ctgaccggat catcttcact cgtcgctgga gccgactcgc ctaacttagc tattccgaag
     2461 tcgagtactt ttacacgata gctgccgagt cgattaggtt ccaaccagat gttatctggc
     2521 tttagatccc gatgaatgat gccgagttga tgagcttcgt gaaccgcaga acaaacctgt
     2581 tcgagaatat ccaccaccca ctcgagcggc agatgatttt cctcggacag aatgtcacca
     2641 agcgtacatc catcgagata ctccattacg aggtacgcaa cctgaccttc tttaacacgg
     2701 gaaaaaccga aatcagtaac gtcgactaca ttcgggtgac gtaatcgtcc ggctgcgcgt
     2761 gcttctcgct tgaatcgctc aacaaattcc gcatttcgca tgaatcgcgg ggcaatcaat
     2821 ttaagagcaa catagcggtc ggtacccaga tgtgtcgcaa ggtagacggc gcccatgccg
     2881 ccctgtccta gcaactgttc aaggcgatat ttatcgtcaa gcgtttgtcc gacgtaattt
     2941 tccagtccct gcatgagaac ggtaatgcta caggaaatat tcgacgatgt cttttgtcag
     3001 tggtcaggga tcagggtcag ggcaattata ggggtcagga ggttggtttg tacttatcgt
     3061 ttccggtttg ggttcatttt tcatttttga gacgacatct gatccaatgc aacacatatc
     3121 accgagttct ctaatcgaat agtttcattc tgtgttctgc tccagtttgc gtaaaatgag
     3181 aaatgaaaaa tgagaaatga aaaataaccc aaatggcaaa ccacaactac aagccacccc
     3241 taaccactgt aaattgcctt ttacttccaa tcacacatcc actcctccat accaatcggt
     3301 tgtccgtcgg gcggatcctg ag
//
LOCUS       DASSFP010000348         9333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2182808, whole genome shotgun sequence.
ACCESSION   DASSFP010000348 DASSFP010000000
VERSION     DASSFP010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182808"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFH91_07710"
     CDS             <1..730
                     /locus_tag="VFH91_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6862918.1"
                     /translation="KRGYLGISLESVHDEFAKVYGLPETKGAIVTSVTPTESGQATPA
                     AKAGIQASDIIVEFEGSPVASAPDLIQRVASTPVGQQTTLVFLRDTNGKLEKKTVSVV
                     LSERPVTDDNAKDWVEPTKSTSKSPDPKGNALHLGITLAELTPQLITERKLTGIQGLL
                     VKDIDPDGLVAEIRLPPNNAPALVLGDVINRINRVPVNTLADFQRVLNTLHPGDPIVL
                     NVTSTQRDRNGDRQVPRIVQFTYQ"
     gene            845..2416
                     /locus_tag="VFH91_07715"
     CDS             845..2416
                     /locus_tag="VFH91_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6862919.1"
                     /translation="MAQAKKAVAKTNSKQPTKSVLRSSVKTYHNYINGKWVASESGEM
                     FENINPADTRDIVGRFPLSTNDDIYAAVNAAQNAFDGWRNTPAPKRAELLFRLGEILI
                     RDKEKFTSDMTREMGKVLKEAGGDVQEAIDCTYYTAGEGRRLHGFTTPAEMRDKFAMC
                     IRQPVGLCGLITPWNFPMAIPSWKLIPALICGNTVVIKPAEDTPLSTYNLVKACEEAG
                     IPPGVVNMVSGFGETVGAAMTNHPALRLISFTGSTATGRIVASSCAERNAICSLEMGG
                     KNAIIVMDDADIDLAVEGAIWGAFGTSGQRCTASSRLIVHKKVYRQFVNKLVDHAKGL
                     RIGNGADSKTDVGPVINAEAVEKIMGYIRIGRDEDGAKLACGGKRLTRGDYGHGFFIE
                     PTVFTDASADMRISQEEIFGPVTTVIPTSSLEEAIKIANGVRYGLSAAIYTQNVNRAF
                     HAMNDMYTGIFYVNASTIGAEVHLPFGGTKATGNGHREAGTQVLDIFSEWKSIYVDFS
                     GKLQRAQIDPVESPQ"
     gene            2473..3606
                     /gene="ald"
                     /locus_tag="VFH91_07720"
     CDS             2473..3606
                     /gene="ald"
                     /locus_tag="VFH91_07720"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET6862920.1"
                     /translation="MIVGLPKEIKDNEYRVGLTPAGVRALKDAHHEIVVETNAGGGSG
                     FEDSLYQRAGAQIVDSADEVWARADMIVKVKEPIEPEYPRMREGQMLFTYLHLAPDHE
                     LTKQLVERKVTGIAYETITDRRGTLPLLTPMSEVAGRMAIQVGAHYLEKMSGGRGILL
                     GGVPGVPAARVVIIGGGVVGTNAAKIAVGMGAHVTMIDNNLDRLRELDDIFLSKISTL
                     ASSAYMIHDAISQADLIVGAVLVPGAAAPKLITKNMLKDVPNGAVIVDVAVDQGGCIE
                     TTHPTTHSNPTFYVEGVLHYCVANMPGAVPRTSTFALTNATLPYALKLANKGFHDAIK
                     SDPGLKEGVNTYAGHCTYEAVASAQGIRFTPLNALLSERGVTV"
     gene            complement(3732..4166)
                     /locus_tag="VFH91_07725"
     CDS             complement(3732..4166)
                     /locus_tag="VFH91_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6862921.1"
                     /translation="MISSKEFELVNLKGVHGGDTSEPYRSVLLAEDDPALRRYLEIVL
                     QRAGYEVLSATDGLEAMKLLIARSFDVVVTDAMMPNLNGYELCRFIRSSKHLSALPIV
                     LLSALDPKNAASETEQVNAFLAKPVSPESLVDCVAQLVSSSQ"
     gene            complement(4305..6242)
                     /gene="asnB"
                     /locus_tag="VFH91_07730"
     CDS             complement(4305..6242)
                     /gene="asnB"
                     /locus_tag="VFH91_07730"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6862922.1"
                     /translation="MCGIAGLIDLQSETSIGPMLESIEHRGRDDQGVWTSSDDGNGRR
                     VSLGHRRLAIIDTSPAGHEPMLTADGRYVLTFNGEIYNYKELRQQLQSLGHRFHTDSD
                     AEVLLTSFAEWDSACVNRLNGMFAFGIWDNKERSLTLVRDHVGIKPLYYTTVAPANGQ
                     PARFAFGSEIKAILASGIVRPVLDPESLNQFLTFLWAPDPNTLFSGIKTVPPGHILKW
                     QDDTITLTRWWDVSFDNIDDRHKEDWWKELTLATLDRVVRMEMVSDVPLGSFLSGGID
                     SSGIVAMMERHRDGRRISTYTAGIKSEDLRYDIIPDDVRWARRVNEQLKTDYHEIILQ
                     PSVVDLLPKLVRHMEEPPIDMAISSYLISTAARETLTVMLSGMGGDEVFAGYPRQLAM
                     KLASAFDPVPSLLRGPLMKTVAGVLPGGLPGRFTAPLRNAKKFARSAALDFEDRYLGF
                     GTYFSDQTKHRMYSNEWRELTAQYDPYSTHRAYFAEAGNAAPLNRLLYVDLKTFLPCL
                     NLMTTDKTSMAANLEVRVPFLNREMIEMVARMPPRLKLRGLKRKYILKRALEAVLPAD
                     VVWRRKAGFGAPIRSWLRGPLRPMVADLLSEERIKSRGIFRPREVKRIVDGNFSGRED
                     NNLQVFQLLGLELWHQAFIDS"
     gene            complement(6307..8460)
                     /locus_tag="VFH91_07735"
     CDS             complement(6307..8460)
                     /locus_tag="VFH91_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HET6862923.1"
                     /translation="MKQVLQNRKTGRPFVGDVPVPALQQGRVLVRTVSSLISAGTERA
                     AVELVSKGLVQEARERPDLVKAVIAKVKSEGLRNTFASVRDKMAASQSLGYSASGVVC
                     EVAHDVTEFQIGDRVACAGLGFASHAEVLSVPQNLCVHLPDAVSFDSGAFGTLGAIAL
                     QGVRLAEPTLGESVVVIGLGLLGQLAVQLLKANGCRVLGLDLSPDKVQLALELGADAA
                     AIASEESEQLVDLWTRGRGADAVLITAASESNGPVELAAKISRLKGRVVVVGMTGLNI
                     PRQPFYMRELKLIISMSYGPGRYDRDYEELGHDYPFGYVRWTEKRNIEAFLDLIDQGR
                     LSVDRLITHRFKIDEAERAYQLISGQIDEPYLGVLLKYDPDAEVNRRIRLLPNSSSTP
                     TSQQGVVIGLIGAGGYVPAMLLPHFKAEKAVFRVIATASGLSAHDVGRKFDFAEAVSS
                     ADEVLDDPDINLIVIGTRHDSHAELVQKALGRGKNVFVEKPLALNDQQLDRVIEQAQA
                     SDRVLLVGFNRRFSPLTIRAKEFFSNSGTPLSMVYRINAGRMPKEHWIHDPEQGGRII
                     GEVCHFVDLMQYLTGSTPVSVFAESTAAKSDTIVDADSVLITIRFADGSNGMIAYLGE
                     GDRALPKERIEIFGGRKAFVLEDFLKATAYADGRAEEFTLRVQDKGQKAQVQAVCSCI
                     VEGKPAPIALNELVSTTRTTFRIVDSLREKNLVSV"
     gene            complement(8476..>9333)
                     /locus_tag="VFH91_07740"
     CDS             complement(8476..>9333)
                     /locus_tag="VFH91_07740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6862924.1"
                     /translation="LARRRGMAHPILWIAIPTAAEMIGSFSESVIVYQVSDKYDANTM
                     DHSTDPALIRKLHEHAIDAADIVFYSGRKLFSEASRGCEYSYLLEQGVDYSHWSTVSS
                     GQIEVAAEIAQIPHPRLGYFGAIEPWLVDQELIKRAAQVRPEWQWIFIGNCSRGLEIE
                     NLPNTHFLPPVRYEELPRYAAGFDVCVLPWETERAFTSYGSAIKVREYLATGKPVVIS
                     PLPEYEPMSDVLRIARSRDEFVSLVEEALHEDDPSLAIKRQKSVASGTWDARAEWVSD
                     LIEEALVKK"
BASE COUNT         2388 a         2475 c         2308 g         2162 t
ORIGIN      
        1 caaacgcggt tatctcggca tatcacttga atcagtccat gacgagtttg caaaagtcta
       61 cggacttcca gagaccaaag gcgcaatcgt cacttcagta acaccaactg aaagtggaca
      121 agctacgccg gcagctaaag ctggtattca agctagcgat atcattgttg aatttgaagg
      181 tagtccagtt gcgagcgcac cagatcttat tcaacgagtt gcgagcactc cggtaggtca
      241 gcaaaccacg ctcgtgttct tgcgtgacac taacggtaag ctcgagaaaa agaccgtcag
      301 tgtcgtactc agcgaacgac cagttactga cgacaatgcg aaggactggg ttgaaccaac
      361 taaatcaact tctaagagtc ctgatccaaa gggcaacgca ttgcatcttg gaattaccct
      421 ggctgagtta acaccacaat tgatcactga aagaaaattg accggtattc agggtttatt
      481 ggttaaagac attgatccgg acgggcttgt ggcagagatt cgcctgcctc cgaacaacgc
      541 gcccgcgctt gtgctgggtg acgtgatcaa ccgaatcaac cgtgttccag tgaatactct
      601 ggctgatttc caacgcgtgc taaacacact ccacccaggt gatccaatcg ttttgaatgt
      661 gaccagcacg cagcgtgatc gaaatggaga tcgacaggta ccgcgaatag ttcagttcac
      721 gtatcaataa ctttggtaaa cctaaagcag gtgctgagcc gtcgtgccag ttgttcatga
      781 aacgcacgcg cgggcacctg actcttcgat agaaagatct gcgtttagaa atcggaaact
      841 agttatggct caggcaaaaa aagctgtcgc aaaaactaac agcaaacaac cgactaaaag
      901 tgttttacgt tcatcagtaa agacctatca caactacatt aatggcaaat gggtcgcgag
      961 cgaatcaggc gagatgtttg aaaacatcaa tccagcagat acgcgcgaca ttgttggccg
     1021 ctttccatta tctacaaacg acgacatcta cgctgctgta aacgccgccc aaaatgcatt
     1081 tgacgggtgg cgtaatactc cagcgcctaa acgtgccgag ttgttatttc gtttgggtga
     1141 gattctgatt cgtgacaaag agaagttcac gtcagatatg acccgagaaa tgggcaaggt
     1201 cctaaaagag gccggcggag acgttcagga agcaatcgac tgtacctatt acaccgccgg
     1261 tgaaggtcga cgactgcatg gctttacgac ccccgcagaa atgcgcgaca agttcgctat
     1321 gtgcattcgc cagcctgttg gcctgtgcgg attgataaca ccgtggaatt tcccgatggc
     1381 aattccatca tggaaactaa ttcccgcact tatttgtggg aacacggttg tgatcaaacc
     1441 agctgaggac actccgcttt cgacttacaa tctcgtcaaa gcttgcgaag aagcgggaat
     1501 tccaccaggc gtagtaaaca tggtgagcgg tttcggcgaa accgttggag cggcaatgac
     1561 caatcatccg gccttacgat tgatatcgtt cactggatcg actgcaactg gtcgcatcgt
     1621 cgcctcatcg tgcgccgaga gaaatgccat ctgttcgctc gagatgggcg gcaaaaatgc
     1681 cattatcgtt atggacgatg ctgacatcga tctagcagtc gaaggcgcca tctggggcgc
     1741 ctttggtacg tctgggcaac gttgcacggc ctcgtcacga ttgatagttc acaagaaggt
     1801 ctatcgacaa tttgtaaaca agctagtcga ccacgccaaa gggttgcgca ttgggaacgg
     1861 ggccgattca aaaaccgatg ttggtccggt aatcaatgct gaagcggtcg aaaaaatcat
     1921 gggctacata cgcattggtc gcgatgaaga tggagcaaag ctggcctgcg gtggaaaaag
     1981 gttaaccaga ggcgattacg gacacgggtt ctttatcgag ccaacagttt ttacggatgc
     2041 cagtgctgac atgagaattt ctcaggaaga gatctttggt ccagtaacaa ctgtcattcc
     2101 caccagctca cttgaagaag ccatcaagat tgcaaacgga gttcgatacg gactgtctgc
     2161 tgcgatttac acacagaatg tgaaccgggc gtttcatgca atgaatgata tgtacaccgg
     2221 tatcttctac gtaaatgcat caacgattgg tgctgaagtc catctgccgt ttggtggcac
     2281 caaagcgacc ggcaatggtc accgtgaagc cggtactcag gtgctggata tattttctga
     2341 atggaaatca atctacgtcg acttcagtgg taaattacaa cgtgcgcaaa tcgaccccgt
     2401 ggaatcgcct caatagaaaa gcacacgtct cataagtcga gtataaacca acaattttgc
     2461 ggaggatgaa ctgtgattgt cgggctaccc aaagagatta aggataacga atatcgagtt
     2521 ggcttaacac ctgccggtgt gcgcgcgctc aaagatgcgc accacgaaat cgttgtcgaa
     2581 acaaacgcgg gaggcggctc aggtttcgaa gactctctat atcaacgcgc cggcgcacaa
     2641 attgtagatt ccgctgacga ggtttgggcc agggcggaca tgattgtgaa ggtcaaagaa
     2701 ccgattgagc ccgagtaccc cagaatgcgc gaaggccaaa tgctcttcac ttaccttcac
     2761 ctcgctccag atcatgaact aacaaaacag ctggtcgagc gaaaagtaac cggcatcgcg
     2821 tatgaaacta tcactgatcg acgcggaact cttccattgt taaccccaat gtcagaggtc
     2881 gccggtcgaa tggccatcca ggttggcgcg cattacttgg aaaagatgag tggaggccgg
     2941 ggtatcttgc tgggcggagt tccgggcgtt ccggccgctc gcgtagttat tatcggcggt
     3001 ggagtagtcg gcacgaacgc tgccaaaatt gccgtgggta tgggagctca cgtcactatg
     3061 atcgacaaca accttgatcg cttacgagaa ttggacgaca tcttcctctc aaaaatctcg
     3121 acgctcgcat caagtgctta catgattcac gatgcaatct cacaagccga tctgatcgtt
     3181 ggtgcggtgc ttgtgcccgg cgcagctgca ccgaaactaa ttacaaagaa catgttgaaa
     3241 gatgttccga acggcgccgt gattgtggac gtggcggtgg accagggcgg atgcatcgag
     3301 actacacatc caaccaccca ttcgaacccg actttctacg tcgaaggagt tctgcattac
     3361 tgcgttgcaa atatgcctgg agcagtgcca cgcacgtcga cgtttgcgct caccaacgcg
     3421 actttaccgt atgcactaaa gctggctaat aagggatttc acgatgcgat taagagtgat
     3481 ccagggttga aggaaggcgt taacacttac gctggccact gtacctacga ggcagtcgct
     3541 tcagcccagg gtatacggtt tacccctctg aatgcgcttt taagtgagcg cggcgtaact
     3601 gtgtgaggaa tgtccgtggt ccgtggtccg ttgtcagtgg tccgtggtcc gtggtccgtt
     3661 gctcttcagg tatagtccac agacatatct ggttcgccac tgacaacaga ctacggacaa
     3721 ctgacaacgg actactgact actactgact aattgagcca cacaatcaac tagactttcc
     3781 ggtgatacag gtttcgcgag gaaggcattt acctgctccg tctcagaagc ggcgttctta
     3841 ggatccaacg cgctgaggag cacaattggt aacgctgata ggtgtttgct gcttcggata
     3901 aaacggcaaa gctcataacc attgagattc ggcatcatcg cgtcagtcac aaccacatca
     3961 aacgaacgtg cgatcaacaa cttcatcgcc tcgagaccat cagtcgcact cagcacctcg
     4021 taccccgccc gctggagcac tatctccagg taacgtcgca aggccggatc gtcttccgcc
     4081 agcaagacag accgataggg ctcagacgtg tcgccgccat gaactccttt taaattaact
     4141 aattcgaatt ccttggatga aatcataacc gggggatttt ggggctcgct ccgaaggccg
     4201 tgagggctta gaagggaggc gcgcctgaac aggctcaccg tgtgaagagt agcccttgaa
     4261 ctcggtgatt gtcaatgttt tcgactgttg aacagcgcct gtaactaact gtcgataaac
     4321 gcttgatgcc acaattcaag gccgagtaat tgaaaaacct gcaggttgtt gtcttctctt
     4381 cccgagaaat tgccgtcaac tatacgttta acctctcgcg gcctgaaaat gccgcgactc
     4441 ttaattctct cctcggaaag cagatcggcc accatcggcc gcaacggacc gcgcaaccag
     4501 gatcgaatgg gagcaccaaa acccgccttc cttcgccaaa ccacgtcggc tggaaggact
     4561 gcctcgagcg ctcgcttcag gatgtatttg cgtttcaaac ctcgcagctt tagccggggc
     4621 ggcatcctcg ccaccatttc aatcatttcc cggtttaaaa atggcacgcg aacttccaaa
     4681 tttgccgcca tcgaggtctt gtcggtcgtc atcaagttta agcagggaag aaacgtcttc
     4741 agatctacat acagcaggcg atttagcggc gccgcgttac ctgcttctgc gaagtaggcg
     4801 cgatgtgttg agtaaggatc gtattgagcg gtcaattcgc gccactcatt cgaatacatc
     4861 cggtgttttg tctgatcact gaagtaagtt ccgaagccta aataccgatc ttcaaagtct
     4921 agcgccgcgc ttcgagcaaa cttcttggcg ttcctcagcg gcgccgtgaa tcgacctgga
     4981 agtcctccag gaagaacacc cgcaacggtc ttcatcaatg gcccgcgcaa gagacttgga
     5041 accgggtcga acgccgaggc cagcttcatt gccagttgtc gcggataacc cgcaaacact
     5101 tcgtcgccac ccatacccga caacatcact gtcaacgtct cgcgagccgc agtcgaaatc
     5161 aggtagctgg aaatcgccat atcaattgga ggctcttcca tgtggcgaac gagtttggga
     5221 agaaggtcca cgactgatgg ctgcaagatg atctcgtgat aatcagtctt tagttgctca
     5281 ttaacacgac gagcccagcg tacgtcgtcc ggaataatgt cgtagcgaag atcctcactt
     5341 ttaatccccg ctgtgtaagt acttatgcga cgtccatcgc ggtgacgttc catcatcgca
     5401 acgattccgg atgaatcgat tcctcctgaa agaaaagatc caagcggaac atcggagacc
     5461 atttccattc tgacaactct atccaacgta gcgagtgtga gttctttcca ccaatcctct
     5521 ttatgtctgt catcgatatt gtcgaacgac acgtcccacc acctggttaa cgtaattgtg
     5581 tcgtcttgcc atttgagaat gtgacctggt ggaacagtct tgataccact aaacaacgtg
     5641 tttggatcgg gcgcccaaag aaacgtcaaa aactggttca aggattccgg gtcaagaaca
     5701 ggtcttacaa ttcccgatgc gaggattgcc ttgatctcgg aaccaaacgc aaatcttgcg
     5761 ggctggccat tcgcaggtgc gacggttgtg taatagagag gcttaatgcc gacgtggtcg
     5821 cgaaccaggg tgaggcttct ttccttattg tcccaaatgc caaacgcaaa catgccgtta
     5881 aggcggttga cacacgcgct atcccattcg gcgaatgacg tcaggagtac ctcggcatcg
     5941 ctatcagtat ggaaacggtg gcctagtgat tgaagttgtt gacggagctc cttgtaattg
     6001 tagatttccc cgttgaaggt caacacgtaa cgaccatccg cagtcagcat tggttcatga
     6061 cccgcgggac tcgtgtcgat gattgcgagc cgacgatggc ccaatgacac tcttcgtcca
     6121 ttgccatcgt cggacgaagt ccaaactccc tgatcatcgc gaccccgatg ctcaatactt
     6181 tcgagcatgg ggccgatgga agtctcactc tgaagatcga ttaaaccagc gatgccgcac
     6241 atactttgga gtgcgccggc ccggcggcgc tttgtgatcg tgacatcacg cctgaatctc
     6301 taatccttaa acagaaacca gattcttttc tcgcagcgaa tcgacaatcc gaaacgttgt
     6361 gcgagtggtg ctcaccaatt cgttcaaagc gattggtgcg ggttttcctt ccacgataca
     6421 actacacacg gcctgcacct gggctttctg tcccttatcc tgaacgcgaa gtgtaaactc
     6481 ttcagcgcga ccgtccgcat aagctgttgc tttaagaaaa tcttccagaa cgaatgcttt
     6541 gcgtccacca aaaatctcga ttcgctcctt cggcaaagcc cggtcacctt cgcctagata
     6601 tgcaatcatg ccgttggaac catcggcgaa tcggattgtt atcaacaccg aatcagcatc
     6661 cacgatcgtg tcggatttag cggcggtcga ttcggcaaat actgaaacgg gagttgagcc
     6721 ggttagatac tgcataagat cgacaaagtg acaaacttcg ccgatgattc ggcctccctg
     6781 ctctggatca tgaatccagt gttctttggg cattcttccc gcattgattc ggtagaccat
     6841 tgagagcggg gtacctgaat tggagaaaaa ttcttttgcc cgaatcgtta acggtgaaaa
     6901 gcgtcgattg aagccaacca gcaacacacg atctgacgct tgagcctgct cgattactcg
     6961 atcgagttgt tggtcattca aagcaagcgg cttttccacg aacacatttt tgcctcgacc
     7021 cagggccttc tggactagtt ctgcgtgtga atcgtgacga gtcccaatca cgatgagatt
     7081 gatatccgga tcatctaaaa cctcatcagc gctcgaaacg gcttcggcaa aatcaaactt
     7141 cctcccgacg tcatgagctg acaatcctga cgccgtcgca ataacacgaa acacagcttt
     7201 ttcggccttg aaatgcggca gaagcattgc cgggacataa ccaccggccc caatcaatcc
     7261 gatgacaaca ccttgctgcg aagtcggagt tgagctcgaa ttgggcagta atcgaattct
     7321 ccggttcacc tccgcatccg ggtcatactt tagaagaact cccagataag gctcgtcgat
     7381 ctgacctgaa atcaactgat aagctcgctc ggcttcatcg atcttaaatc gatgtgtgat
     7441 caaacgatcg acactcaaac ggccctgatc gatcaagtcc agaaatgctt cgatattccg
     7501 cttttcggtc caacgcacat aaccaaatgg ataatcgtgt ccgagctctt cgtaatcccg
     7561 atcataccgg cctggtccat acgacatcga gattattaac ttaagctcgc gcatgtagaa
     7621 gggctggcga ggtatgttca gaccggtcat gcccaccacc acaactcggc ctttgagtcg
     7681 cgagattttt gccgccaatt ctaccgggcc gtttgactca cttgccgcag ttatcaggac
     7741 tgcatcagcg ccacgtcctc gagtccaaag gtcgaccaac tgttcagact cttcagatgc
     7801 tattgctgcg gcatctgcac ccagctcaag tgcgagttgg actttgtccg gcgatagatc
     7861 aagaccaaga actctacatc cgttcgcttt taatagttgg accgccaatt gcccgagcag
     7921 tcccagccca atcacaacaa ctgactcgcc taatgttggc tcggccaaac gaactccctg
     7981 aagcgctatt gctcccagag ttccgaacgc cccgctgtcg aagctcactg cgtcgggaag
     8041 atgaacacaa agattttgcg gcaccgagag cacttcggcg tgcgaagcaa agccaagccc
     8101 agcgcaggca actcgatcgc caatctgaaa ttcagtgacg tcgtgagcaa cctcgcaaac
     8161 aactcctgaa gcgctgtacc cgagactttg cgaggccgcc attttgtcgc ggaccgaagc
     8221 aaaggtattt cggagtcctt ctgacttaac tttggcaatt actgctttaa ccagatccgg
     8281 acgttcacgg gcctcctgga ccaagccttt actgactagt tcgacggcgg ctcgttcggt
     8341 gcccgcacta atcaatgacg acacagtgcg cacgagcact cggccctgct gaagtgctgg
     8401 tacaggcacg tccccgacaa atggtctgcc agtttttcga ttctgaagta cttgtttcat
     8461 tcgcagttga atggcctact tctttaccag agcctcctca attagatcac tcacccattc
     8521 tgctcgagcg tcccaggtgc ctgacgctac agacttttgc cgttttatag caagcgacgg
     8581 atcgtcctcg tgcaatgcct cctcgacaag cgacacgaat tcgtcgcgcg agcgggcaat
     8641 tcgtaacaca tcgctcatcg gttcatattc aggaagcggc gaaatgacca caggcttacc
     8701 tgtcgccagg tattcgcgta ccttgattgc tgaaccgtag ctcgtaaaag ctcgctcagt
     8761 ttcccatggg aggacgcaaa cgtcgaatcc ggccgcatat cgaggcaatt cttcataacg
     8821 aaccgggggc agaaagtggg tgtttggtaa gttttcaatc tcgaggcctc gggaacaatt
     8881 gccaataaaa atccactgcc actcaggcct cacttgagct gcgcgcttaa taagctcctg
     8941 gtctacgagc cacggttcga tggcaccgaa atacccgaga cgcggatgag gaatttgtgc
     9001 aatctccgca gccacttcga tctgacctga gctcaccgta ctccaatgtg aataatcgac
     9061 accttgctcc agaaggtaag agtattcaca ccctcgcgaa gcctcagaaa agagtttgcg
     9121 accggaataa aaaactatat ccgcagcgtc aattgcgtgc tcatggagtt ttcttatcag
     9181 ggcgggatcg gtcgaatggt ccatggtgtt ggcatcgtac ttatcggaaa cctggtaaac
     9241 aatcacggat tccgaaaaag agccgatcat ttccgctgcc gtgggaatcg ctatccacag
     9301 gatgggatgg gccataccac gccgcctcgc cag
//
LOCUS       DASSFP010000349         5394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1129559, whole genome shotgun sequence.
ACCESSION   DASSFP010000349 DASSFP010000000
VERSION     DASSFP010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129559"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..1156)
                     /locus_tag="VFH91_07745"
     CDS             complement(332..1156)
                     /locus_tag="VFH91_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6862925.1"
                     /translation="MTDGHGAATDTRSLIQVDIDNGIAQVKLNDPPANTYSYEMMQQL
                     DRAILDLRMDESVQVIVITGAGEKFFCAGADIRMLANATPTYKYNFCLHANETLSRLE
                     QTPKLVIAAINGHCVGGGLEVAMAADMRIARKGAGKLGLPEVSLGVLPGTGGTQRLTR
                     IVGKSKAIELMVTGELFDFERGLDLGILNQIVEADTGEEFLKQVSQYAAQFTAPNKAS
                     LAVGRIKRSVQTGAEISFESALALEREMQQQLFQSADAREGIDAYVSKRKPEFTGK"
     gene            complement(1198..1884)
                     /locus_tag="VFH91_07750"
     CDS             complement(1198..1884)
                     /locus_tag="VFH91_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6862926.1"
                     /translation="MSNTATDFSRYDQKLEFILRTAAGIFAEKSYHSTSMRDISRATN
                     VSLAGLYHYCSSKEELLFLIQDNCFGRVLARLEERLKEIEDPVVKLRLFIENHLSFFA
                     ANMAEMKVLSHEAESLRGDLHAHVSGKKDKYTKLARHILSELDQAQKQEERVDLTVAT
                     YALFGMMNWIYNWYDPAGKLTVAELARNLTQLFLIGFAPASFSPQFAAHSATGQKENL
                     SIWRGSDSVR"
     gene            complement(1933..3330)
                     /gene="boxB"
                     /locus_tag="VFH91_07755"
     CDS             complement(1933..3330)
                     /gene="boxB"
                     /locus_tag="VFH91_07755"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HET6862927.1"
                     /translation="MNLYDRIPNNVNLAEDKKLQRALEKWLPNYIDWWREMGPDGFQE
                     KEVWLRTAVDVGSEGWAHFDYVRMPEYRWGIFLEPPQPDAKVGFGDNYGRPAYQEVPG
                     EFRNSLRRIIVTQGDTEPASVEQQRQLGKTCPSLYDMRNLFQVNVEEGRHLWAMVYLL
                     HAYFGRDGRDEADALLERRSGNPDKPRILEAFNQPCDDWLNFFAFTMFTDRDGKYQLS
                     ALAESGFEPLARTCQFMLTEEAHHMFVGDTGLGRVVRRTAQLMKEGDVRELGGIPLEI
                     IQKYINFWFSYSLDLFGGEISSNSADFFAAGLKGRYKEREQYQDHSAASEMFTLMVVE
                     DGHLNPREVPLRNALNELLRTEYVKDCEKGLARWNKVLVDEGATERLYLPNPRFHRHV
                     GEYAGHSFDLKGVLISQEEFEKRKADWLPTLEDREYVRSLMSPVTEPGKIANWIAPPA
                     AGIKGKPFEFEYVRL"
     gene            complement(3502..5130)
                     /gene="boxC"
                     /locus_tag="VFH91_07760"
     CDS             complement(3502..5130)
                     /gene="boxC"
                     /locus_tag="VFH91_07760"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HET6862928.1"
                     /translation="MINFQTSPDQYHHWKLQLNGAVAVLSMDVQEDETLAEGYKLKLN
                     SYDLGVDIELADVVQRLRFEHPEVQVVVITSLKPRIFCAGANIYMLGTSSHTFKVNFC
                     KFTNETRCAIEDDSRHSGRRYLAALNGTASGGGYELAIACDEIYLVDDGNSAVSLPEV
                     PLLGVLPGTGGLTRLVDKRRVRRDRADVFSTLAEGLKGKRAKDWGLIDGYFPTSKFQE
                     AIDRRVSEVVRDGSGNTTGIKLNPLSIEQGDSSRDYEYVKVKLNREQRYADLMIHGPN
                     DDQPTTLAEIENLGDRYWPLQVYRELDDALLHLRVNEPLIGLVCVRTVGSADRVLSVD
                     QTLAANHDHWLVREILLNMARVLRRMDLTAKSFFAIVEPGSCFAGNLLELLLASDRSY
                     MLNDPENDETRLAVSELNAGPLPMSNGLTRLQSRYLADPERLEEIFNGAGKFDVDAAE
                     ELGLITFAPDELDWEDEIRLAIEERTSLSPDALTGMEASLRFAGPETMDTKIYGRLTA
                     WQNWIFQRPNAVGEKGALTNYGKPTQAQFDYKRT"
BASE COUNT         1270 a         1392 c         1297 g         1435 t
ORIGIN      
        1 gtgataacta ctacgcgagt tgagcgttca cttgcggcat gctcgagtgc ttcagcgaga
       61 tctcgacgca tatgtccgac gaaggcattg agtttatcgg gacggttcag tgtgatcgtc
      121 acgatgcctt cggattcttt aatctcaatg tggtccatag ggatttgttg atttgtactt
      181 tgtgctttgc acttcgtgct ttgtaccttg tgcttcgttg tggaccctct tcaaccgccg
      241 gccactgaac catggacgat agtaacgacg aacaacgaac aaagcactaa gtacgaagtg
      301 caaagtgcaa agtgtaatag gcccgaagcg actacttccc ggtaaattca ggcttgcgct
      361 tcgaaacgta ggcatcaata ccttcgcgag catccgccga ctgaaagagc tgttgttgca
      421 tctcgcgctc taacgccaac gctgattcaa atgagatttc agcacctgtt tgcacgctgc
      481 gcttaattcg gcccacagcc agcgaggctt tgtttggtgc cgtgaattgg gcggcgtatt
      541 gactaacctg cttgagaaat tcttcgcccg tatcagcttc aacgatctga ttcaagatcc
      601 ctaagtcgag tccccgctca aagtcaaaca actcaccggt aaccatcaat tcaatcgcct
      661 ttgactttcc aacgatcctg gttagtcgtt gtgtgcctcc tgttcctgga agaacaccga
      721 gcgagacttc cggcaaccca agtttgccag ccccttttcg tgcaatacgc atgtcggccg
      781 ccatcgcaac ctctaaacct ccgccaacac aatgaccatt gattgcggca ataacgagct
      841 tcggagtctg ctccagccgc gagagcgttt cgtttgcatg taaacagaag ttgtacttat
      901 aagttggggt ggcatttgcg agcatgcgga tgtcggcacc cgcacagaaa aacttctcac
      961 cggcgccggt gatgacgatg acctgcacgc tctcgtccat tcgcaaatcc agaatggccc
     1021 tatcgagctg ttgcatcatt tcataactgt aagtattggc cggtggatca ttcagtttta
     1081 cttgtgcgat cccgttgtca atatcgactt gtatcagcga ccgagtgtcg gttgcggctc
     1141 catgcccgtc tgtcatcgtt aatctccact cgaaaggatt tagctggttt taatttgcta
     1201 tctaacacta tctgagccac gccagatgct caggttctcc ttctgcccgg ttgccgaatg
     1261 agcggcaaac tggggagaaa aacttgcggg tgcaaagcca attaagaata gttgagtcaa
     1321 gtttctggcc agttctgcca ccgtcaattt tccggcggga tcgtaccagt tgtagatcca
     1381 attcatcatt ccgaatagcg catacgtcgc caccgtcaga tccacgcgtt cttcttgctt
     1441 ttgcgcctgg tccagttcgc tcaagatgtg acgcgcgagt ttagtgtatt tgtctttctt
     1501 acctgaaacg tgcgcgtgaa ggtcgcctcg taacgattca gcttcgtgag acagaacttt
     1561 catctcagcc atgttggcgg caaagaatga gaggtgattt tcaataaaga gccgaagctt
     1621 aacgacagga tcctcaatct ctttaagtcg ttcttccaaa cgtgcgagga cccgtccaaa
     1681 gcagttgtcc tgaataagaa atagtaattc ttccttcgag gaacaataat gatagaggcc
     1741 cgcgaggctc acgttcgtag cgcgcgatat atcacgcatc gatgtcgagt gatagctctt
     1801 ttcggcaaag attcccgccg ccgtgcgcag gatgaactcc agcttttgat cgtaccggga
     1861 aaagtctgtt gcagtgttac tcataacatc ctgttcgaag tgatgctgaa acgagagacg
     1921 cccagaactc tctcaaagcc tcacatactc aaactcgaat ggcttacctt ttatgccagc
     1981 agccggcggc gcaatccaat tggcaatctt accgggctct gtcacgggtg acatcaggct
     2041 gcgaacatac tcgcgatcct cgagggtggg cagccagtca gccttacgtt tttcaaattc
     2101 ttcctgagat ataagaacgc ccttgaggtc aaaagagtgt ccggcatatt cgccaacatg
     2161 acgatggaat ctcggattgg gaagataaag acgctcagta gcaccttcgt caacaagaac
     2221 cttattccaa cgggccaaac ccttttcaca gtctttgaca tactccgtgc gaagaagttc
     2281 attcaaagca tttctgagag gaacctctct tggatttaag tgtccatctt caaccaccat
     2341 caaagtgaac atctcgctgg ctgctgaatg gtcctgatat tgttctcgct ctttatatcg
     2401 gcctttcaag ccagcggcaa aaaaatcggc tgagttggaa ctaatctcac caccaaacag
     2461 atcgagagag tagctaaacc agaaattgat gtacttctga attatttcga gcggtatacc
     2521 gccgagttcg cgaacatctc cttctttcat caattgcgcc gtacgtctga caacgcggcc
     2581 caggccagta tcgccaacaa acatgtgatg agcttcttcg gtcagcatga actggcaagt
     2641 tctagccagc ggctcgaatc cggattcagc cagggcagac aactgatact taccatctcg
     2701 gtcggtaaac attgtgaacg caaagaagtt caaccaatca tcacaaggct ggttaaatgc
     2761 ttccagtatc cgaggcttat ccggattacc gctgcggcgt tccaaaagtg cgtcagcttc
     2821 gtcgcgcccg tccctgccaa aatatgcgtg aagcagatag accattgccc acaggtgtcg
     2881 accttcttcc acgttgacct gaaaaaggtt gcgcatgtcg tagagcgaag gacaggtttt
     2941 gcctaactgt cgctgctgtt cgactgatgc aggttcggta tctccctggg tcacgatgat
     3001 ccgccgcaaa gagtttcgaa actcgccggg cacttcctga taggcgggcc ttccgtagtt
     3061 gtcgccgaat ccaactttcg cgtcaggctg cggtggttca agaaaaatac cccaacggta
     3121 ttcaggcatc cggacgtagt caaaatgggc ccaaccttct gaaccaacgt caaccgccgt
     3181 gcgaagccaa acttcctttt cttgaaagcc gtcaggtccc atttcacgcc accaatcgat
     3241 gtagttgggt aaccactttt cgagagcgcg ctggagtttc ttgtcctcag cgaggtttac
     3301 gttatttgga atgcgatcgt ataggttcat tgtgtgtagt ggtcttaggt cttaggtctt
     3361 aggcctttgg tttttggtct ttgttctttg ttctgtgatc attgattgtt actcttggtc
     3421 cgtcatgtaa cttctctctc tgacctaaga cctaagacct aagaccaaag gccgaaggcc
     3481 gaagaccaaa gtccaaggtt tttaagtcct cttgtaatca aattgtgcct gcgtgggttt
     3541 cccgtaattt gtcagcgcac ccttttcgcc gacagcgtta ggacgttgaa aaatccagtt
     3601 ttgccaggct gtcaatcgtc cgtagatctt ggtgtccatc gtttccggtc ccgcaaatct
     3661 cagtgatgct tccattcctg ttaatgcatc aggcgacaaa gaagtccgct cttcaatggc
     3721 cagccttatt tcgtcttccc aatccagttc gtcgggtgca aaagtgatca atccaagctc
     3781 ttcggctgcg tctacgtcaa atttgccggc cccgttaaag atctcttcaa gcctttcagg
     3841 atcggcgaga tatcgtgact gcaatcttgt tagaccattg ctcattggca atggaccagc
     3901 attcagttct gacaccgcga gtcttgtttc atcattttca ggatcattca gcatgtaact
     3961 tcgatctgag gccaacaaaa gctccagcaa attgccggcg aaacagctgc cgggttcaac
     4021 gatcgcgaaa aagctttttg ccgtcaggtc catccgtcgc agtacgcgcg ccatgttgag
     4081 taagatttcc cggacgagcc agtggtcgtg atttgctgca agtgtttggt ccaccgatag
     4141 cacacggtct gcgctgccaa ctgttcgtac gcaaactagt cctatcaacg gttcattcac
     4201 tcgcaagtga agcaacgcgt cgtccagttc gcgatagacc tgcaacggcc agtagcggtc
     4261 accaaggttt tcgatctcgg ctaatgtcgt aggctgatcg tcatttggtc catggatcat
     4321 tagatctgca tatcgttgtt cgcgattcag cttcactttt acatactcgt aatcacgaga
     4381 gctatctcct tgttcaatcg agaggggatt gagttttatt ccagttgtat taccgcttcc
     4441 gtcccttacg acttcgctga cgcgtcgatc aattgcttcc tggaacttac tggtcgggaa
     4501 atagccatcg atcagtcccc aatcttttgc acgcttgccc ttcaggcctt cggctaaggt
     4561 tgaaaagaca tcggcgcgat ctcgacgcac tcttcgttta tctacgagac gagttagacc
     4621 acctgtgccg ggcaaaactc cgaggagcgg aacctccggt agcgacacag cggagttacc
     4681 gtcatcgact aggtagattt catcgcaggc gattgcgagt tcgtagcccc ctccagacgc
     4741 ggttccattc agcgcggcca ggtagcgacg gccggagtgt cgcgaatcgt cttcaattgc
     4801 gcagcgggtc tcgttagtaa acttgcaaaa attgacttta aaggtgtgcg acgatgtccc
     4861 gagcatataa atattggcgc cggcgcaaaa tattcggggc ttaaggctcg taatgacaac
     4921 tacctggact tcagggtgtt caaaacggag gcgctgaaca acatcggcca actcgatatc
     4981 gacgcccaga tcgtaggaat ttagttttag cttatagcct tcagccaggg tttcgtcttc
     5041 ctgaacatcc atggacagga cggcaactgc tccgttaagt tgtagtttcc agtggtggta
     5101 ctggtcaggg gacgtctgga agttaatcat ttaggatcct cagtagcttt gagccgcttt
     5161 ttactacgtt cgaacgttcg ttcgaataat agtctggagg gcttgggttg gtcaaatggt
     5221 ggctgtatgt gctttccact ttgcccctga tacctggtgc ttggtacttt gtcatagcct
     5281 gaaatttgag tttggacgtg aaatgaatcc gatcacgttg aagggcacaa agtgcaaatg
     5341 cgttggcaaa aaacaatcct tacgataatc ctcataattg gggcgctggc gatg
//
LOCUS       DASSFP010000350        41860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2183080, whole genome shotgun sequence.
ACCESSION   DASSFP010000350 DASSFP010000000
VERSION     DASSFP010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 41860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41860
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183080"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFH91_07765"
     CDS             <1..238
                     /locus_tag="VFH91_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6862929.1"
                     /translation="NTDDPDEPVFASASVNLRKVWEAVRKEAGIPTVRFHDLRHTFAT
                     RMADAGISMTYRYHHLTKKVNDKMRDILNKRAAM"
     gene            complement(814..1104)
                     /locus_tag="VFH91_07770"
     CDS             complement(814..1104)
                     /locus_tag="VFH91_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862930.1"
                     /translation="MIGELKLRLARKQLDFEHERYRNRIIGLDKQVRLAAGTGGYTSL
                     PKHARIMGYPNNQKAGTNPAACLICDIFYALAASVSISVWVLVVPVLSPIRF"
     gene            1332..1733
                     /locus_tag="VFH91_07775"
     CDS             1332..1733
                     /locus_tag="VFH91_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET6862931.1"
                     /translation="MRFLLGVLVGYGLRGKRKLLIKVLVTLLVVCVVLSAVVLPAIAL
                     LALRLDVQRERRSRPVQTKVPVLKGLTYEDAERKLHAANLNIRLLATRHEPTTQPGLV
                     IDQTPAPGEEVAVGYSVGVTINKKDYAGPGP"
     gene            1881..2177
                     /locus_tag="VFH91_07780"
     CDS             1881..2177
                     /locus_tag="VFH91_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862932.1"
                     /translation="MKNVFSLRKRSFLSPVSTGYTSHIFAEVESSNEGEYRWGHNMLT
                     IADCRRRVQIEFFLGTKRERRRSLAKINLLINILTRFRDVLTKEIALIEKVKAK"
     gene            2310..2705
                     /locus_tag="VFH91_07785"
     CDS             2310..2705
                     /locus_tag="VFH91_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862933.1"
                     /translation="MPTTKHELLDWLMDVPEDAEIGTDGAGLALLAILGTNVHLLEVG
                     NIPNADELYAEAINQAMMERLRRIHAAGGETETGVIIVTLQGYISGIPSLLSTDFNTA
                     FIFANREQAEAFITEFAEELHNPQILDCP"
     gene            2739..3050
                     /locus_tag="VFH91_07790"
     CDS             2739..3050
                     /locus_tag="VFH91_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6862934.1"
                     /translation="MGQPPYTQSFAQINSSLNLKTMGHSRPRPKHLAKKLLQIRRSLG
                     ISQGEVIRQLGVQALIDHTTISKYELDKNEPPLVILLAYARLIGIPVEQIIDDELELT
                     I"
     gene            complement(3465..4772)
                     /locus_tag="VFH91_07795"
     CDS             complement(3465..4772)
                     /locus_tag="VFH91_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862935.1"
                     /translation="MPTVAEWIFALTTEYEVKVVSSPQLISDVTAKQNRRIAEFAAKK
                     LPPPAMKQTLTQISQFSPSDTIKVAPLTLLHAIGRVTTPCIPNLADISSTWALLRYVW
                     AFEPPSSGTAEPLRLSSVAREIDFHQKSLLSDELGIGITYYLMTNFFAAPNVIDVDIA
                     LRTPTWDLDRVLPTIPDYIFFDTPTGTLHIVECKGNQTTYSNALDQLRRGTEQVPSII
                     TTTGQTIIALIVATYLMPDRTEVYIIDPPGDENEDGRLSLESLMNNNSNDPPRKAEKI
                     SETAWKIIDYKRFVNELVQFHRAKVLTFIGADAEALRIIPGNVPDKRTRFVAKESPLD
                     TVRTDLGVFRGVTEIIPNRHEIEVQLFRGVLDQLYTIYVSNGAMAEEARILKSFRRHL
                     KQSQGTNRGVGYSFFRKKQRNKTLLQSISTDGTMLQVTLSESK"
     gene            complement(4800..6125)
                     /locus_tag="VFH91_07800"
     CDS             complement(4800..6125)
                     /locus_tag="VFH91_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862936.1"
                     /translation="MSRDLPEQPNLEHLKKQARDLLRAYNQGEAVAAQRLSSRTSHVT
                     KVQLADAQYAIAREYGFGSWMKLKQHVESRASVKTVWFPEPSVEDDSIARHGERTYSW
                     LSRSTSAKAKGCRRFLNENISHVPAQWQGKLHNDFRTKEWSNVFFELIVARTLQLLGA
                     SIEVEVQIAETGKRPDFTAHFSDGEVTVEATIPEINEEMSRQSAENEELVQIIEGLTP
                     AAWTVSVWRLPRLGPNDSKREFKQMIREIFAGLPANPSEEQKPIEIELNYGEFMLTIS
                     PGRKGKRAAVVRGMAAGPDNTEQKIRAIVSRKKKQVRGARTPVLLAVSTSPFGEREDY
                     DRALFGLTFERVDQYGKTVATGFDPVGVFGANRPEPPTCAGVLAFMEVGFPGVADPVL
                     YLHPRFKGVIPESLKRLERRTLRVGNGIHVERASIHNFTSSLGFVSRFG"
     gene            6515..7192
                     /locus_tag="VFH91_07805"
     CDS             6515..7192
                     /locus_tag="VFH91_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6862937.1"
                     /translation="MSENLDQIAFPADDIFVNPEPRCPCLLLLDTSSSMRGQPIQELN
                     EGVTLFKDELMADSMAAKRVEIAIISFGPVKVDLDFQTPDVFQPPTLHASGDTPMGSA
                     IEQGLELLQQRKQVYKANGITHYRPWIFLITDGGPTDSWQHAATLIKAGEESKSFAFR
                     AIGVEHANMEILAQIAVREPLRLKGLRFRDFFLWLSNSLSSVSRSTPGDEVPLVNPTA
                     PDGWASA"
     gene            7192..8037
                     /locus_tag="VFH91_07810"
     CDS             7192..8037
                     /locus_tag="VFH91_07810"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET6862938.1"
                     /translation="MYDHTWKCIFASEAGTSHSATNSSCQDNCICEVIEDSAGSKVMA
                     VVTSDGAGSAKHAEAGSALTCSFFIEQLKAHLASGATVRELTRNHVESWISKLQDELT
                     LRAEAEDGHSVRDFAATLLAAFVDENSAVFFQIGDGAIVIPGDDPETYGWIFWPQQGQ
                     YANETNFVTDQNAQDKLEFTVINHRVDEVAVFTDGVQSFALHYQSRQAHSPFFFSVFS
                     WLRSAPCDRLDEFRKALISFLKSEKVNERTDDDKTLALATRVQLTSSDPLDSNNASED
                     RLPRL"
     gene            8006..10678
                     /locus_tag="VFH91_07815"
     CDS             8006..10678
                     /locus_tag="VFH91_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862939.1"
                     /translation="MPLRTGSHVYDSHGRKVTLADKLAQGGEGAVFDLLNQRDQVAKI
                     YHLPFDDQKARKLTSMVGLKTERLLKLAAWPIDTLHERPGGRMIGLLMAKAVGHKEVH
                     KLYNIKSRLIEFPHAQWPFLIYAATNIARAVNVIHEHGHVIGDINHASVGISNKATAT
                     LFDCDSFQIMTPDCEYLCEVGTDFFTPPELQGMSFRGLVRSKNHDAFGLAVIIFQLLF
                     LGRHPFAGQFLGQNETSSLPKFIREYRFAYGPTATSRQMRQPPGTLDLEAVSPGIAQL
                     FEKAFQPSRQRPLPTEWTSGLEQLVKALTQCKINPSHHFWKGLASCPWCKIEAQALIV
                     IFAQVDVVIDQTGTFNLTMTWSLIESVPPPPALPPLPKSKDFHLTRSVRSSKIYRSNL
                     LRALATTAALFALSAIVLASPISATSSAWIIVIAGGIALTIANSGNNDSRRQLESERR
                     AAYARLHEAERRWQEQAGSKRFDEKGQQLSAQKTEYLGLAALRQRKLNDLQSTIRQRQ
                     LYKFLDRHLISHAHISGIGPGRQAILSSYGIDTAADIDRKAIAKVYGFGPVYTSKLLS
                     WRNSIEQRFVFNPAIGIDPADKRALETEIATTRAKLEKELQSGPSELRRINQQILIAR
                     SVIFPVVETNIKAAAQADLDASTNLSRAIGLAPVLLTLGVALILIFSLKVNRSRQSFS
                     KSAAAVPTATPTPTSTPTPSLAQLKADAQQHYMAGLQLTRARKYVEAATAYQQAIKLN
                     PALSEAHHELAFAFYRLGEYERSIAAAENAIKVRSDNPDTYRILGWSLTGMRRWKEAM
                     EAFEHSLQLDPDSSATYYSLGMAAKGNAEIDSALEAFKEAISLKPDYAAAHFELGQLY
                     LDLGETQMATQEYETLIPLNPKLAQRLAKRLGLE"
     gene            10832..14581
                     /locus_tag="VFH91_07820"
     CDS             10832..14581
                     /locus_tag="VFH91_07820"
                     /inference="COORDINATES: protein motif:HMM:NF045198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862940.1"
                     /translation="MTKLTQIENALLASNEARFQELCDRYLYREGYKQINRIGAKVGK
                     EKTTKGRPDTLISLSNGRHVFVESTTTQKDLFDKLMSDVESCFDENQTGVPVDKIERI
                     ILCHNSKLTAAEQEALTSRCQQEGCLLDILGIASLSFGLLEKHQVLAKEYLGIELDSG
                     QILSPEDFIELYQKSAFATRLDLNFHSRKEEVKAALEALDKNDLLIITGPAGVGKSRF
                     ALEYIHKFIEQDVSYQSFCVFNKRLPLYEDLKVYFGNDGHYLLFVDDANRLSQLDPLL
                     QLLHDQRKNRKIKVILTVRDYALPVVRKTAREYSSKVEFYLSPLKNEEIREIIEAEFG
                     IRNHYYLDRICKIAQGNPRLAVMAAMLAKETNKFESISDASKLYDEYFSSITADFSEL
                     ETAKLLQIGGILAFFGSLDRTTELFKQVAESFGLSESELWEGLERLHASELVDVYDTE
                     VAKISDQVLGTYLIYAAFFKLESSDFSVLLSDYLASYEYKFREAVYPILDIFNHQFVL
                     QRLQKHIDIKWKAVDGDESQLLRLMRVFWFVKKTEILVYLKDKIDSLPATKVDFATLT
                     FEPASQGLPKDQYLEVLEAFQYAAGEDFRIALELILEYLEKQPSLLSHVLYLLLERFS
                     FDVDSYDRAYRTQRELVDVLIRKSNDPETGRLHQRLLIRIAQNYLKTQFRSHRSEGLN
                     VLIQEFQLLPSPELLQLRKSLWLFIAKASRESATREFAFQVIVDHATRFHDTPVNKPT
                     NQIVKLDSEVLLPFLSSTLDPSSYADVLVMQQYLRFINQHKVRIESKLQKQFTNETYR
                     LSKILLPEWDIDFDKRRTNAIKKEVAGYSYRDYLKFLERCIDIQKYIKRDVDAYQLMS
                     AIDKVLVNLSEMDASLFKKVFRSLLQTGNKLGHIYGTAIVKLRLSYSSPRHAYNLLKR
                     YEYSLKESWLLAFLSQLGPEQVNGFYLNELYSLYRTADLRLFTDFDFLESYRRIDKDV
                     FVNAVKIVFDRSQPKQSANFHYLFNPHTYVSKNLKNLFAENIELLEELYLHQSAIDRL
                     VNYRGLVLKKIVELDPAFLTKYLQWLYGEQDYVSDPMDGARYIALWTLDNYQTVIAEA
                     VEFVFEKEKSDYARSFNYINAFFRQDDESFSSECKPSVTERMETFISEYIQTHHQDRQ
                     RMIFIFDVITECFPAKRVRFLKLFLSLNQDYETFERLAIQPNRWGGEGSLVPVFEKKI
                     EFLQSILPLVSSVKFLKHKLHVNNLILEWKERIEIENKKQFIGYF"
     gene            14650..14892
                     /locus_tag="VFH91_07825"
     CDS             14650..14892
                     /locus_tag="VFH91_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862941.1"
                     /translation="MSNRANADRLPSGRELFKVIEVVDSEAWNNLVRISDAVTIFHIT
                     SWQDCSPHAFIRFGTYQGDRLHAGALVEVNEFGADK"
     gene            15147..16931
                     /locus_tag="VFH91_07830"
     CDS             15147..16931
                     /locus_tag="VFH91_07830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6862942.1"
                     /translation="MNRIVAQLLSFEGEVVYDRCTYEEFTSEIDLAQRMEKNSAFLSN
                     YVHVKSIGKGFFATVTHYKEKSTGRDVAVKKLKDEQYAYRFEREIKIMQALDGHPNII
                     KLIDFECVSGSYQHITSKAETNLYNHIKKRNATLTLEQRIRIFDQVLAAVSYAHSKKI
                     LHRDIAPNNVLIFDSEHIEVCDFGMGKDLSTLSNYTTSIVAQYGQVYYVAPEQRDKLN
                     AASERSDIYSLGKLLNFILTGRDPDIIHPSDFNSIIAKATQYEPNDRYQNILEFETAY
                     NRLKSLLLLDTTSTLPKTLLDFKGDNGSIDWQEFHKLAARSDHEGHPYSDYVDPIVQV
                     LSRPGNLQQYYEAVGDSFVDFLTTFIGKLNELAGMTGWPFSETKTFARFLRDIFFGVR
                     EPALKLVCLKHIWDLAYEQDQWGAQSIMEGLLADDHIPIDIQTSFAVHILDSSIGSLP
                     DSSKSTIPRIIKQALAQKANELDAQRRLASLRAVLKALNDNERDLLKLFASTRSESDE
                     FDHPFLPYNVYDSVNNLISNGVLTREQKAGLGEHEQLISLSPEAVNLVETLIIEDKVR
                     RESIVLDRRNIAASGASGSGAPPHLNHR"
     gene            17023..17175
                     /locus_tag="VFH91_07835"
     CDS             17023..17175
                     /locus_tag="VFH91_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862943.1"
                     /translation="MPTFPAAEPLLRVSRIPLRNFSGDKDQNDLQHARRYIPSPHETY
                     FEKIFS"
     gene            17403..20846
                     /locus_tag="VFH91_07840"
     CDS             17403..20846
                     /locus_tag="VFH91_07840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6862944.1"
                     /translation="MTQPKLNPLSRSAIVFVILMLFRVTSLAQFQTAQISGFISDKHG
                     ATLLGAAVKIVNADTREEHQTLTDKTGYFIFTNLMPGKYEIAVEITGFQKFLQGEVNV
                     DAHSKVRVDASLEPKGVNETVNVVDARPNIQRDTAVIGKTIERRDIQDLAVNGRNPIN
                     FVLLAPGVRGGPFNSFNPDDLGAIGFSINGSRLEENLITVDGAIAIRTRASGAIIGIP
                     NADSVQELQVFTSNYLPEYGRSSGGQIRFTTRVGGRDFHGSVFEFFRNSALDANSWGR
                     NASNNLDIARKPAPFTFNQFGYDIGGPVYVPRRFKVDREKLFFFWAQEWIVWRRFDTS
                     TQTVPTQKMRTGDFSELLNPNNPFFARAVFIRDPLKAGECSANDQTSCFPGNVIPLER
                     LSPNGVALLKTYPLPTPGFQRGVSNYIAQSPNPRDTQKDTLRIDYIIGSNNQLSFRGS
                     LFNWDSVDAFRGSFPLARTDWRRPNRSAALSWTSQLSPSLINELTLSASVDRVFINVF
                     NNGLYQREQYGINYPYLFPGKEIENKIPTVTISNFGTVDGGPYPSKSSGPIYTLSNNI
                     TRVTGQHSVKTGVFIEYSGENDFDQINVQPVPGGTNNQNGRFEFTNARTNGTGLAVAN
                     AALGLFTNYGEIGQRSYTPWRAWSTDLFIQDTWRARRNLTLEYGTRYSYWQPWRSLWG
                     NIAMFDPAFYDRARAAAIDSVGGFLINPTFESRFNGIVLPGRSWPEAALGRVNAASDP
                     RFQSLFHGLPEGFSATHKNVFEPRAGIAYSFHNKTVVRAAAGIFHNRVTLNDSTLLGG
                     NAPIQFMTGVTDGLADAPGGATMRTFPLVVTMQDPEFKHPTSYQYNFTIQRELPFKTT
                     IDIAYVGRIGLYLQRERNINQLLPGTRQANPGVNTDALRPFKGFGVIRLSENAGRSDY
                     NGLQLSVRRRFQRGVSFGATYTFSKLTDDASNRRERLFNAYDAHSFRGTSSDDRTHVF
                     NVHYVYEIPFWREQNTLVKKALGGWQVSGVGLVVSGQPLTVCRGDDDAGVGDSDCQPW
                     DLVGDTHVESPSFSRGRTVDQNFWFNPTAFRRPAPGTFGNAGRNILRGPTFQSWDIAI
                     SKTFSIDEGKRLQFRSEFFNFPNHPNLANPDTNPTSPNFGRVLGKAGERNIQFGLKFY
                     F"
     gene            complement(20988..21266)
                     /locus_tag="VFH91_07845"
     CDS             complement(20988..21266)
                     /locus_tag="VFH91_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862945.1"
                     /translation="MLVYFVYKGERMNRVRTLLLISLVLVACAPVFAQRSEMIAYPST
                     WREEKEKSRFNPPDWVVLFSLPKNAEIHLVYSVGGPKEVGLFDKRASQ"
     gene            complement(21637..22476)
                     /locus_tag="VFH91_07850"
     CDS             complement(21637..22476)
                     /locus_tag="VFH91_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862946.1"
                     /translation="MSINSLEVVKRKALSFLLILPVLLVNSLLDSPQVKAAVPVPSGF
                     QLILSDTGVKVYRKNYVGGQPDFVTVVDLRYATIRNFTGSVSGQNIERRTLSRFWNDA
                     VAQNTGSRTARVAINGTFFSTNDSPFTPIAFGLKADWWRMSYGYGINEYPGLVRTFAF
                     DSFYGSSSIQPYSTATFDAGIPDVVGGLDPTANKSASSYIGRTFVGVRDDDNNGHSET
                     VVFFSSNYATQAGAVSVLSGFGTGSKMMLDGGGSTGLIIGGTSYISPTRSIPHAIIVC
                     AGK"
     gene            complement(22552..22986)
                     /locus_tag="VFH91_07855"
     CDS             complement(22552..22986)
                     /locus_tag="VFH91_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862947.1"
                     /translation="MQLVKIFSCLLALTVFIGAFVSANAQGSGDQWVEREKGNTNMAF
                     PVSITKPRDARECGGGWKDRLLPDRILIFNTPNAGRGNPDDIRWWSNMPDVVVAMGGS
                     LAASGLTTPTTRVCIGFKSHLSGKDYQYLYLWRKQASGPRRP"
     gene            complement(23058..23825)
                     /locus_tag="VFH91_07860"
     CDS             complement(23058..23825)
                     /locus_tag="VFH91_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862948.1"
                     /translation="MKVHTRLSLIAVIAFCFGGQVTTFAQNVTVPREPTPTETEGPKA
                     PSAGQTLVRDLIGAIARVVCEHSGKCLPGSDTPTQTETPTEKGLGRGRTPIESNRTGR
                     SAEPSYTLAPRPSFSYFTPSGWRAFEERSSITVAPQSEYTNGNLANGVIFGLADLNGA
                     SFESGTDRYVRGILSANKYLKRIGRSESSVINNVPCITSRLAGPSPQSGYTENVEIYT
                     CQHSAENLFYVVNVTSGPNANLYLQENSRVIQSISFR"
     gene            24112..24705
                     /locus_tag="VFH91_07865"
     CDS             24112..24705
                     /locus_tag="VFH91_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6862949.1"
                     /translation="MSESEPEKTFASNEITRLLNAWAGGRHSAADELFPLIQRELHKI
                     AKEHMRRQDPGHILQTTALVNEAYVRLAGSKDKTWKDRNHFYAVASMAMRHVLVDYAR
                     KELRDKRGSGKSDVPLDDATEIPNEPSSMLIALDEALRSFAKLDPRAAQVVELRYFGG
                     LSIKETASVLGVSPATVSNDWAAAQLWLTRELSENKK"
     gene            24702..26537
                     /locus_tag="VFH91_07870"
     CDS             24702..26537
                     /locus_tag="VFH91_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6862950.1"
                     /translation="MNADQWQRVKELCVAALTFAPPKREAYLEQECDGDLRLLDEVRS
                     LLAVQSKASNCLGELPREFVAAAFENHVSLVGRKIGNYDVRTLLGRGGMGEVYLAEDR
                     DLRRKVALKVLPEELTRDTHPLQRFKQEARAASGLKHRNIITIYSVGQEDGIYFIAAE
                     YVNGLTLRQRLMSSGRLELKDAIEIGIQIGSGLEAAHNAGIVHRDVKPENIMFESDGT
                     AKLLDFGIAKLTETLGPENVSSTQPFKTEQGIILGTPAYMSPEQARHQSLDHRSDLFS
                     LGVVLYEMITGNVPFSGGTPYDQQAAVLVGEPTPINQHLDKFVPELQWVLSKALTKEP
                     GRRYQTATDFLIDLQMLHQRLQDEDVDNKKSDVTDATSSRVTVIADNRPLWKRRWTLT
                     AIVLLGLVAALAAVMISTRAGQRLFGLIGPPPVIESIVPTRPIDVIGNQPIELRGRNF
                     EDGLKLKVGFPNGGSGELSGAQIQKQEGNSTTLNILIDLNGNPGIYTLQLLNPDGQVS
                     LPFNFEARHETQRPSINWIEPTVVKKSGGEQVIAVYGSNFQQGVSVDVVQPNGETSQL
                     SGRQIANQTPNSFHGLFFFYLPGKYSIRVINPSGGKSEYYDIVVQ"
     gene            27162..27905
                     /locus_tag="VFH91_07875"
     CDS             27162..27905
                     /locus_tag="VFH91_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862951.1"
                     /translation="MNDRYGGMSKIKSSKSKSENTLKREIRALRSTLNRVWATLVDID
                     EATPLEEALDAIDEACEIMAADWPNVFEITDEVSPKVQQQIDLENQVNKLAEEAQRTD
                     NWGTRAVLFRRFLALAAGLSDEQFEQQIQQLTPDEYRDLALRNLWMIGAITAPTPIDY
                     KDQTDRLIRTLKEFKKSLPRKHGPEPSPVILEAVRIRDTENKSYKEIYNRLIPIFGEE
                     MRNLTPVTLSARVRTRRSRLRRSRNSDQS"
     gene            28034..28717
                     /locus_tag="VFH91_07880"
     CDS             28034..28717
                     /locus_tag="VFH91_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862952.1"
                     /translation="MAQIFISHSRRDEQTKNFFYRTFSGTAVLPIWMEYEDAATSSID
                     QTIERRIHGSNAVFILLSETVERIPFTRDWILWENGKITNKDIWVFEPEDSLGRINVV
                     LPRFHHYVRDRRTKSWRDYINSIVRSYDSSNLVSLAGTGAGVGAILSDEDRAAGAIVG
                     GLVATIIEAIGRPKAPTGFPVSCLNCSYNYAVHLTRGVNQFRCARCNCILELRSQEFL
                     SVNRIEWAH"
     gene            complement(28763..30559)
                     /locus_tag="VFH91_07885"
     CDS             complement(28763..30559)
                     /locus_tag="VFH91_07885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6862953.1"
                     /translation="MEQAITVSSTWEEHTYLAEQRCSCGGDYSALRWELDFQDEQSFE
                     SVQAKCNSCGLETQFWFDITAALQPQPQDYIRKAWQVPRDGPYSIDEKGYREAVAKLA
                     SGSADTVEEDFRRGSKAILDKYFRRIAGRFQVHGLRHGGMGTAYLCVDTEWSPGSSLP
                     YFVVCKTIESSSGNVEIEALKSEARIWSYLGGHPNIVQLFDVIALSPSRLVLVMEAVL
                     PGPLGVTTVKEWIDHDLVSLPLALRFFSGLCKAMVHCQLQAPGFVHGDLKPENLLVAR
                     GHELKVTDLGLARARGFNDPFSKQTGGTFLYLAPECWEGTERSEKSDVYAAGVVGYEM
                     LTGKCPFERKTSRGGLNALDRRQKVPPLDDPLIPVSIRNLLAQCLAQDPSRRPTFNDL
                     LQGLGNDDLCACELDSSKTAAEWNNKGKALAQLGQHRRATDCYLRAVSLEPSNPVGWN
                     NFAISLSILGDNARAEQSYTLSLALGENSAQTFANYAAHLVREGNPARLEEALSLCEK
                     SLRLDSQNLSGLINKAAILNGLGRYPEAVKAAERATKIEPSHPHAWVELGTAYWKCGR
                     RSKALKCAKQALKLDPTFGPAHTLKRLLDTRA"
     gene            30938..33214
                     /locus_tag="VFH91_07890"
     CDS             30938..33214
                     /locus_tag="VFH91_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862954.1"
                     /translation="MRIIPQQLSFDAKNEAVCLHLSQISTSLNQTRKKIKNPTEQWQL
                     IEAILRSVSSTADPSHVNFLILPEIAVPYHYVMNLFEIVSETFPKNSITIAGIELSTV
                     DECKNLASRLKSVEASTLLQTAESERLVNQCLIITKERNKCRPLLQLKMTPSKFEGGV
                     VTAESLLSSDYLYYFQTNGPIGVSFVTLICSDFFNRPDGALSKIIDEVDFQILKRGRA
                     LDFLFNIQHNRSPDNELFLHGLRRIYDDGYKAHGDLCTIFLNAALDGDKGGRSKILFY
                     KGNLLPERQPIKQLDAPVVGYELSGAQTLSSVHFKRLPRSWDNRRDTYPVDFTCYKFS
                     EKQWTTNKDSKIVYLAPMQTEPYLDHRSYKELAERLSVLGRFDDSIHWAKKRLSHSEE
                     RKDFHEAALAALFMAIQFRHKGELTEALKHYAHAEAHILSLTKLTPGAALTHWRIQLG
                     RIMVEDFMINGRCKQAHDNYQTFGREIDEYLSAQGSSDHELGWRREIEQIKLYKIHLS
                     RQCAEMQRLLGRYKRARSLFDAAYKQYSYAYGEEKAYSALGCADSLRMMGRYSEAIDK
                     YEEVREFATIKGQRRLLARVLRNRAEILRIEDTWDVLNTTLEELTGLSNETDYRFGKI
                     YIPLIRGAARLRIDPKQAIEIFSQIESLVSRFSQNYLKVEFAHYLFGIAEARRLCGER
                     ESQQFYERAHKLYKETGVLWGIIRTSVGLSLVSNSLNGFEQSLKGLTISDPLDRSFIE
                     RAQQGKLDKKDILLLNIP"
     gene            33218..34294
                     /locus_tag="VFH91_07895"
     CDS             33218..34294
                     /locus_tag="VFH91_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862955.1"
                     /translation="MAQIILAEFENVKGNIQSDLPYDLALAECLSSKSDALSKADLNE
                     TSYAVKGVAECYRRLGLTAKAIREYKTALSLFRETANVRGIAWSKWGIGICERQQGDY
                     LQSIHTLRKAKRHALLSNDNRCVAYTCAGIAEAVRILGHYKVALLQHYTVLERFKSLR
                     DLRGIVWALEGIAQIHKNSGRYHDALRLFDTSKKIASETIDFRGLGYALKGSGECLSL
                     LGLYEPAVNDLASAVRLFELINFRLGLAYAYKTLGDAYLRSDSLAEAHANYSNAMPLF
                     RQINEDRGIAFLRKSIGDFNLRVGSLDEAYDNYLNSQTFFREKGVRYGVHISTVALNG
                     ASTNLSRSYSVPCDAAKRKRSTSS"
     gene            complement(34513..34941)
                     /locus_tag="VFH91_07900"
     CDS             complement(34513..34941)
                     /locus_tag="VFH91_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6862956.1"
                     /translation="MICPTCGADISEIAGPQVFCPKCSARVDDDAEYCSSCAARISAT
                     DVMNCPNCGKSVDAEAKFCKYCAADLQRTNAQSLSNISDDAITGNLASRQRAQTSTSA
                     NLVFFGIVLAIVSAAAYLWGVNYAGNFSNAMAASLDKRQA"
     gene            34891..38421
                     /locus_tag="VFH91_07905"
     CDS             34891..38421
                     /locus_tag="VFH91_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862957.1"
                     /translation="MRTSNFRDICSASRTNHICARNLGAADLDVVRTQLLTEIVTKFR
                     NKRVVIIHAASGQGKTALSYRYLHEYFPQSWRFQVSSVFDREQALRVALALKAHADAL
                     GVPMVIYLDVSPQDRDWPELVRSLSTHHNLQVLVSIREEDWKRAGILVMGFEFETVEL
                     SFKRNEAVTIYDSLVQKHPPANVLNAEEAWERFGGAGPLMEFIYLVTQGDSLRTKLSQ
                     QITYLKNEVREKRLDVSEIEFLRLVSIASASGARLRVKPLAEHLNLPAPQSTLELFEK
                     EYLLRVSAEGIHVQGLHPIRSTMLTELLTDATFAPWFEGAVSCLPFVEEADLEIFLLH
                     CFSQHREHRNELLYSLKFIKPISWIGIIGVVRALIWAGVAEYVEANRKLIEDAVAQIG
                     IGGWSYFIDFDIANVSDSTAASWWSELDFITDEVREKIKELQSHQTDKKQVFQHATSW
                     LTGYSIKPVPPTTEADWSAIAETAFWVGRLNVGWPLPQSLTETELEWAIEKLSLEILA
                     NLVSGLFENEEFHNWFLTNRKRLISRFRQDTDTVMLEDDGHRLTARFIFALPDLVDAP
                     ADDTQNISAKTKNSFHDAAIQRLGLLRSLFPDREEFSTQGYGHMLWENFLEFDETIKT
                     GVDRKHLPLVWLTSVNATFRGLGNLQFRPATWLEYSGRILDIRRSVLSSLKQLERGIE
                     NHFRRTRVTKMLGTDVNGEQWDRCKMMLTHTPLLPRVAVDEWGFLEESTSDINNRGIA
                     ERLTQRSSIAAGRYKAFLKAFNEHTRTLSNFFGQATDCMITNALLGRASDRTKVLEAA
                     SSVGIKPNSCKLSTLNFADSLKNLRAFQREARTILNPFFDDQELNRLDEEEQRSLHRI
                     WCAWYFFAHQPQKVMQNAVSVSERECLQILRTIRLDLRGGLRRISSDLVRFNILSEDT
                     LWDDRPALWLSVDADSPLAALSSLPTVLDQVRQSIQKVAKSDLRRYVLDLNWPHVVLV
                     PLVKGKCPNTVAWRIALPVITESKNESLSWWNYAQSPIPTEAVKHLKIDSWNLPQLSV
                     AAKLMQYTTILFYIAVHIRDFCRLPDLDEEGLTQLQSYVSRLSRHASEALQSVLDTEV
                     EMLAAFNNLSEGDQKLRPYLFESAVAITNLHKAILPSEDFSNRLEMDLEGLIDWATRL
                     EQAPAHALAASSAWMADVIDQDLSDQAPSG"
     gene            38522..39217
                     /locus_tag="VFH91_07910"
     CDS             38522..39217
                     /locus_tag="VFH91_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HET6862958.1"
                     /translation="MTDIELAIATQTEFEEFLRHTLDATGNSFAELIMSIKERLTRSL
                     VDDLHYLRRERNSLAHRRKRELDSRQKFEEVSSRARESLLRVAVPTLSDIGYSIVNKK
                     SGKSLDVPWEMNNYIVHQWSYHCGTNQHWCLRRTAEKHIAIVSRYTGRCLDVERSSDS
                     DNAAVTQWKYHGGLNQQWILTRLEDHSYQIRAAHSGKVLDIVWASDDDGATTTQYPWH
                     GGDNQRWWIGAAL"
     gene            39853..40104
                     /locus_tag="VFH91_07915"
     CDS             39853..40104
                     /locus_tag="VFH91_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862959.1"
                     /translation="MADCRRRIQLEFFLGTVRARRESLRKIDLLMKQLEQFRTALRTE
                     AGLIEQYEAKQKAKPRKSNKASKRKAVPNGRTNKRSHAE"
     gene            40094..40300
                     /locus_tag="VFH91_07920"
     CDS             40094..40300
                     /locus_tag="VFH91_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862960.1"
                     /translation="MRNNVIASTHAEMPYATVKLTPSEIRPRDRVFLQQWAEKLGVSI
                     DVLLKGILLAAIEDQLYAEKIPEI"
     gene            complement(41630..>41860)
                     /locus_tag="VFH91_07925"
     CDS             complement(41630..>41860)
                     /locus_tag="VFH91_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862961.1"
                     /translation="QRASQRVLNGMVNPRDLRNTAAAIRADRSKRRGAILTRLGYTRG
                     WATRFNYLETFLLEPKRTPRRHQPKRQPPAGA"
BASE COUNT        10995 a        10304 c        10032 g        10529 t
ORIGIN      
        1 gaacaccgac gaccccgacg aaccggtgtt cgcaagcgcc agcgtgaacc tacgcaaggt
       61 gtgggaggct gtacgtaagg aggccggtat ccctacagtg cgctttcatg acttgcggca
      121 cacgtttgca actcgcatgg ccgacgctgg tatctctatg acctataggt accaccacct
      181 gacgaagaag gtgaacgaca agatgaggga catactcaac aaacgtgcag cgatgtaggt
      241 gaaatggcat agctaagaga ttttatattt tagttttctt ggcggtcagg attagatacc
      301 tgcatggcta gaccgggtac tggttcggag cgtcccttta tgtttttgag cgtctcttag
      361 cgtctgtcag gctcccgcca cgccagctaa cttaatgaca gcaagcctat tagcgttgta
      421 atcggcggtt tatttacagg tactggtgca gcacaggcga aatatctttg aattgtttgc
      481 cgatttagtg catcctatta gcctcggctt gtagccatca agtcgagctg gttgcggcct
      541 caggtagtgc cctttaattg gaaagtggat atgtatatgg aaaaggagaa aaacaatgcg
      601 acaccaaatt acatttacaa ttcttcaaaa tcgacgctta gggtatggac cacactacca
      661 ctgaatctct tcttggtatt ctttggcacg tagtacagat tgaacttttc ttaattctca
      721 taacgctctt tacagtatcg ggacacctaa tcagagaatg gctgactgaa aaggaacccc
      781 aaaaacttga atccaaaatt gaaccgcaga tagttaaaat cgaattggtg ataagacagg
      841 aactaccaga acccaaaccg aaattgatac agaagccgcc aaggcgtaaa agatatcgca
      901 gataagacat gcagccggat tcgttccggc tttttgatta tttgggtacc ccataattct
      961 ggcatgcttc ggaaggctgg tgtaaccacc tgttcccgca gccaacctaa cctgcttatc
     1021 tagtccgatg atgcggttcc tgtacctctc atgttcaaaa tcaagctgtt ttcgggccag
     1081 ccgtaactta agttcgccaa tcaagcttat tctcaagctc cattacaggc tcggtgagat
     1141 actgcgaggg aagcggaatc aggatcaatg caagtttcgc tcattgcgtt gagttaacct
     1201 cccttaccgc gtaattgacg cctctccctc acgctccctt cgaccgacat tctccaacac
     1261 aaccaattag aaaaagaggc ggttaccgag taggtgattc actaggaaat ctccaaaagg
     1321 aaaccgctgt aatgagattt ttactgggcg tcctcgttgg ttacggccta cgaggaaaac
     1381 gcaagctgct tatcaaagtt cttgtgacgc tgctcgtcgt gtgtgtcgtt ttgtcggctg
     1441 tcgttttacc agcaattgcg cttctggcgc tgcgtcttga cgtccaacgt gagcgccggt
     1501 ctagaccagt tcaaactaag gtgccagtcc tcaaaggact gacctacgaa gacgccgaga
     1561 ggaagcttca cgccgctaac ttgaatattc gtctgctagc gacacgtcac gagccgacca
     1621 ctcaacccgg attagttatc gaccaaactc ccgcacctgg tgaggaagtg gctgtcggct
     1681 actctgtcgg agtcaccatc aacaaaaagg attacgcggg tcctggtccg taaaatcaaa
     1741 gctaaataca ttggatcagt aagcccacgc gctgcgttcc tggcgcatcc agcctccctg
     1801 tctttcaata ggcgcgacac ttcaatcgtt ttgcccagag acacggaggt ttcacgtaat
     1861 tatttcctcc gaggtgctac atgaaaaatg ttttctctct tcgcaaacgt tcattcctct
     1921 cgcctgtctc aactggctat acgtcgcata tcttcgctga ggttgaatcc tccaatgagg
     1981 gcgaatacag gtggggtcac aacatgctta ccattgccga ttgccggcgc agagtccaga
     2041 ttgaattctt tttgggcacc aagcgagagc gtcgtcgatc cctggccaaa atcaatttgc
     2101 ttatcaacat ccttacgcgc tttcgtgacg tgctcactaa agagatcgcg ttaatcgaga
     2161 aggtcaaagc caaataggtc acgggaaacg atcgcggtcc aacttgcggc taagccactc
     2221 atatcaggtg aaagctgaaa gcgagttggc ccgcattgtt aatactcact cgaatcaact
     2281 tctgcgcgag aagaaagtga ggcaactaaa tgccaacaac taaacatgaa ctgctcgatt
     2341 ggttaatgga tgttccagaa gatgccgaaa taggaactga cggagccgga ctcgctttgc
     2401 tggcaattct tggcaccaac gttcacttgt tagaggtcgg taacattccc aatgcggacg
     2461 agctctatgc cgaggcaatc aatcaggcaa tgatggaacg tctgcgccgg attcatgcag
     2521 caggtggaga aacggaaaca ggcgtaataa tagtgacgct tcagggctac atatcaggca
     2581 ttcccagttt gttgtctaca gacttcaata cggcgtttat attcgcgaac agagaacagg
     2641 cggaagcctt tatcacagag ttcgcagaag agctacacaa tccgcagatt ctcgattgcc
     2701 cataatccta ccagcggata attctgcact gaattctttt gggccaacct ccctacactc
     2761 agtcgttcgc acaaatcaac tcttcactga atctaaaaac tatgggacac tcgcgacccc
     2821 gtccgaaaca tctcgcaaag aagctgctgc aaatccgacg cagcttagga atatctcaag
     2881 gagaggtgat tagacaactt ggtgttcaag ctctcatcga ccatacgact atttccaagt
     2941 acgaactcga caagaacgag ccgccgctcg ttatcctgct cgcctatgct cggctcatag
     3001 gaattcccgt cgaacaaatc attgatgatg aactcgagct aacaatttaa tctccgcaag
     3061 agtattcgcg atcttttcct accgctaatc actctccaaa cattcgtcga tgctacgctc
     3121 acagtgtcga tgttaaaatt tctgtcaatc actgccgtcg gctaattcaa ttaactccgc
     3181 tccgggactt ttgaccgatc caacaaacaa cgtgccgagc ctccgctgcg tcagccgtca
     3241 agggtctacg ctgcgctccg atgaactccg cgaacccaaa acagttcgct ccgcccttga
     3301 ctgccttcct cgctgcggct taagagtcga taagagctgc taacgcagct ctgaaagcgg
     3361 cgctttcaag cttatggttc aaacggcgtg gactggccgt aaacttgctc gcgtactgga
     3421 ccgaatcttc gacgttggct tgattatccc tgtaagcatt tcgtttactt agattctgac
     3481 agggtgacct gtaacattgt tccatcagta ctgatgcttt gcagaagagt tttgttgcgc
     3541 tgctttttcc taaagaagga ataaccaaca ccgcgattgg tgccttgaga ttgttttagg
     3601 tgtctcctaa atgatttgag aattcttgcc tcttctgcca ttgctccgtt gctcacatat
     3661 atggtgtaaa gctggtcaag tactcctcga aacagctgga cttcgatctc gtgtctatta
     3721 ggaataattt ctgttactcc tcgaaatacg cctagatcag ttctaaccgt gtctagcggt
     3781 gactctttag ctacgaagcg cgttctctta tctggtacgt tccctggaat tattcttagg
     3841 gcttcagcgt cagccccgat gaaagttaac actttagcgc gatggaattg gactagttca
     3901 ttcacaaacc gtttatagtc gattatcttc caagcagttt ctgagatctt ctctgctttg
     3961 cgtgggggat catttgaatt gttgttcatc agtgactcga gagacaatct tccgtcttcg
     4021 ttctcgtccc ccggagggtc aatgatgtac acctcggttc gatcaggcat caaataagtt
     4081 gcaactatca atgcgatgat cgtttgtccg gtcgtcgtta ttatggaagg aacttgctct
     4141 gttcccctcc gaagctgatc aagcgcatta gaatatgtag tttggttgcc tttgcattcc
     4201 acgatgtgaa gcgtacccgt tggcgtatcg aagaagatat agtctggaat tgtcggtaaa
     4261 actctatcga gatcccacgt cggcgttcgc agcgcaatat cgacatcaat aacattgggg
     4321 gcagcgaaga agttcgtcat caagtagtac gttatgccta tccccaactc atctgataat
     4381 aaagattttt ggtgaaagtc gatttcacgt gcaaccgaac ttaaccgaag aggctcggca
     4441 gtgccggagc taggtggttc aaaagcccac acgtaacgca aaagagccca ggttgaactg
     4501 atgtcagcaa ggttcggtat acagggggta gtaacacgcc cgatcgcatg taggagtgtc
     4561 aaaggagcta ccttgatagt atctgaagga gaaaactgcg aaatttgagt cagcgtttgc
     4621 ttcatcgctg gaggggggag tttctttgcg gcaaactccg cgattcgacg gttctgcttt
     4681 gccgtcacgt cggatattag ttgcggagag gaaactactt taacttcgta ctctgtggtc
     4741 aatgcaaaaa tccattcagc aaccgtgggc accgttcttt tcctccacca ttattccggt
     4801 taaccaaatc tcgatacaaa accaagagac gacgtaaagt tatgtatgct cgctcgctcg
     4861 acgtgaatac cgttacccac acgaagagtt cggcgttcca gtcttttcag cgactcaggt
     4921 atcacacctt taaacctcgg atgtaaatac agcactggat cagccacacc aggaaaaccc
     4981 acttccatga acgccagcac gccggcacac gtaggaggtt caggccggtt ggcgccaaag
     5041 accccaaccg gatcgaaacc cgtcgcaacc gttttcccgt attggtcgac acgttcgaag
     5101 gtcaaaccaa aaagagcgcg gtcgtagtct tcacgttctc cgaatggtga ggtacttacc
     5161 gccagcagca ccggcgttct tgctcccctc acttgtttct ttttgcggct aacgatggct
     5221 cggatttttt gttcggtgtt atccggacca gcggccatcc cccgaaccac cgcagcccgc
     5281 tttccttttc gccccggact aatcgttagc atgaattctc catagttcaa ctcaatctct
     5341 attggcttct gctcctcact cggattcgcc ggcagccccg cgaagatttc acgaatcatt
     5401 tgtttgaact ccctcttgga atcatttggg ccaaggcggg gcaatctcca aacggaaacg
     5461 gtccaagctg cgggtgtcag cccctcaatg atttgaacca gctcttcatt ctcagcgctt
     5521 tgccgactca tctcctcatt gatttctggg atcgtcgctt caacagtaac ttcaccatca
     5581 gaaaagtgcg ctgtgaaatc tggtcttttg cctgtctcag cgatctgtac ctcaacctca
     5641 atggaagctc ccaagagttg taacgtgcgc gctacaatca gttcgaaaaa aacgttgctc
     5701 cactctttag tcctgaaatc attgtgtagc ttgccctgcc actgagcggg cacgtggcta
     5761 atattctcgt tcagaaaacg cctacaacct ttagcctttg cgctcgttga tcggctcaac
     5821 caactgtaag ttctttcgcc atgcctggct attgaatcat cctctactga tggttcagga
     5881 aaccaaacgg tcttgacgct tgcccgtgac tcgacatgct gctttagttt catccaactg
     5941 ccaaaaccgt actcacgcgc aatcgcatat tgagcgtcgg caagttgaac cttagtcacg
     6001 tgcgatgttc gcgagctcag tcgctgggca gccacggctt cgccctggtt ataagcgcgt
     6061 aataaatctc tggcctgttt cttgaggtgt tctaggttag gttgttcggg tagatcgcga
     6121 gacatttctc ctccttcttt gtatgcccga tgtccgcacc ataggcagaa gaaaggttaa
     6181 tgctcgtttc ctacgaaact aacagatggg ataatccctt tccgcggacg ggtgcaatct
     6241 gtattgcacc ccgaattgta gcacacaata gcagcgtgtc ctcgcgatgt ttctgctttc
     6301 gatgggctaa gagaacaagt tcatagtact gaaaaccagg ttcgaggcaa ttacacctcg
     6361 atcacttatg cgcttcccga aaaagttaaa attactggtc tctggcaaga gccttgaaag
     6421 cgaatagtct cttgttattt aatggaagag atttttgggt agacactcga acgttcctta
     6481 ccctctttac tcaatcccaa agtgaggctt gcacatgtca gaaaatctcg accagattgc
     6541 tttcccggct gacgacattt tcgtgaatcc tgaaccacga tgcccctgct tgctcttgct
     6601 cgacacctcg agctcgatga ggggacaacc catacaagag ttaaacgaag gagtcacctt
     6661 atttaaggat gaattgatgg cggattcgat ggctgccaaa agggtcgaaa ttgctatcat
     6721 tagtttcggt ccggttaaag tggaccttga tttccaaaca ccggatgtct ttcaacctcc
     6781 tacgcttcac gcgagcggag atactccgat gggcagtgca attgagcaag gcttagagct
     6841 gctccagcaa cgaaaacagg tctataaggc caacggtatt acccactacc gtccttggat
     6901 ctttttgatc actgacggcg gccctactga tagttggcaa cacgctgcaa ctcttattaa
     6961 ggctggtgaa gagtccaagt cgttcgcttt ccgcgccatc ggtgtagaac atgcgaatat
     7021 ggagattctc gcccaaatag cagtgcgcga acccctccgg ctgaagggat tgcggttccg
     7081 tgatttcttt ctgtggctct ccaattcact tagctcagtg tcgcgctcaa cacctggtga
     7141 tgaagttcca cttgttaatc caacggcccc ggatggctgg gcctcagcct gatgtacgat
     7201 catacttgga agtgcatctt cgcctcggag gcaggcacgt cccactccgc gacaaatagc
     7261 tcttgtcagg acaactgcat ttgtgaagtc atagaagaca gtgcagggtc aaaagttatg
     7321 gcagtggtga cttccgatgg agctggtagc gcaaagcatg cagaagcagg ttcggctctt
     7381 acttgctcat tctttatcga acagctcaaa gcccatttgg ccagcggagc gacagttcga
     7441 gaactcacgc ggaatcatgt tgaaagctgg atttcaaaac ttcaggacga actcacttta
     7501 cgagcagagg ctgaggacgg tcattctgtt cgcgactttg ccgcaacgtt actagccgca
     7561 ttcgtagacg agaactccgc agtatttttc caaatcggtg acggcgcaat cgtcatcccg
     7621 ggggatgacc ccgagactta cggttggatc ttttggccac aacaaggcca gtacgcaaac
     7681 gaaacaaatt ttgtcactga ccaaaatgcc caagacaaac tggaattcac ggtgattaac
     7741 caccgcgtcg acgaagtcgc tgttttcacc gatggcgtgc aaagttttgc attgcactac
     7801 caatcacgcc aagcacacag tccattcttc ttctctgttt ttagttggct gcgatccgct
     7861 ccatgcgaca gactggatga atttcgcaaa gcgctcattt ctttcctgaa gtctgagaag
     7921 gtcaacgaac gcactgacga tgacaagacc ttagccctcg cgacgcgcgt acaactcact
     7981 tcgtcagacc cactggattc taacaatgcc tctgaggacc ggctcccacg tttatgacag
     8041 tcacggtcgt aaggtgactc tcgccgacaa gcttgcacag ggaggcgaag gcgctgtctt
     8101 cgacttgctg aaccaacgtg accaggtagc gaagatttat caccttccat tcgacgatca
     8161 aaaagcaaga aaattgacgt cgatggtggg actcaagacc gaacggctgc taaaactggc
     8221 agcgtggcca atcgacactc ttcatgagcg accaggcggg cggatgatcg ggctgttaat
     8281 ggcgaaggcc gtggggcata aagaagtcca caagctctat aatatcaaaa gtcggttgat
     8341 tgaattcccc cacgctcaat ggcccttcct tatatatgca gccacaaata tcgctcgagc
     8401 cgttaacgta atccatgaac atggacatgt tattggcgac ataaaccacg cgagcgttgg
     8461 tatttcaaac aaagccactg caaccctttt cgactgcgac agctttcaga taatgacacc
     8521 agattgcgag tatctgtgtg aagttggaac cgattttttc accccaccgg aactgcaagg
     8581 aatgtcattc cggggtcttg ttagatcaaa gaaccatgat gcattcgggc tcgctgtaat
     8641 catctttcaa ctgttgtttc tgggacgcca ccccttcgct ggtcaattcc tgggtcagaa
     8701 tgaaacttct tcgttaccaa aattcatccg cgagtaccgg ttcgcttatg gtccaaccgc
     8761 tacttccaga cagatgcgac agcctcctgg gaccctcgac ctcgaagcgg tatcgcctgg
     8821 aatcgcacaa ctgtttgaaa aggcttttca accaagtaga caacggcctc ttcctactga
     8881 atggacatcg ggcctagagc aattggtcaa agctctaacg caatgcaaaa tcaaccccag
     8941 tcatcatttt tggaaaggcc ttgcctcatg cccttggtgc aaaatcgaag ctcaagctct
     9001 aattgtgatc ttcgctcaag tcgatgtagt aatcgaccaa accggtacct ttaacctcac
     9061 gatgacctgg tcgctaatag aaagtgttcc gcctcctcct gcattacctc cacttcctaa
     9121 gagtaaagat tttcacttaa cgcgatcggt acgcagttcg aagatctacc gaagcaatct
     9181 tctgagagcg ctggctacta ctgccgccct gttcgccctt tccgcaatag tcctcgcttc
     9241 tcctatcagt gcaacgtcaa gcgcttggat aatcgtcatc gcgggtggaa tcgccctaac
     9301 tatcgccaac agcggcaaca atgattctcg gcgccaactt gaaagtgaaa gacgagcagc
     9361 gtatgcacga ttacatgagg cggagcggcg ctggcaggag caagctggca gcaaacggtt
     9421 cgatgaaaaa gggcaacaac tgagtgctca aaaaaccgag taccttgggt tagctgcatt
     9481 gcgacagcgc aagctaaatg acctgcaaag caccatccgg caacgacagt tatacaagtt
     9541 tcttgatcgc cacctcatta gtcacgcaca cattagtggc attggccccg gccgccaagc
     9601 tatcctcagt tcctacggaa tcgatactgc ggctgacatt gatagaaagg cgattgctaa
     9661 agtctacggc tttggtcccg tctatacatc caaacttctt agttggcgaa attcgataga
     9721 gcaacgattt gttttcaatc cagcgatcgg aattgatccc gccgataagc gggccttaga
     9781 gaccgaaatt gctacgactc gagcaaaact ggagaaggaa ttacagagtg gcccgagtga
     9841 gttgcgtcga atcaaccagc agatccttat agctcgttca gtaatatttc cagtggtaga
     9901 gacaaacatt aaggcagccg ctcaagcgga tcttgatgcc agtactaatc tctcacgcgc
     9961 tattggtctc gcgccagttt tgctgactct tggggtagcc ctgatcctga tcttttcttt
    10021 aaaagtcaat cgttcacgac aaagtttctc aaagtcagcc gctgcagttc cgaccgccac
    10081 tcctacaccg actagcaccc cgactccctc tctggcacag cttaaggctg atgctcaaca
    10141 acactatatg gcgggcctgc aactaaccag agctcgcaag tacgttgaag cagcaacggc
    10201 ctatcaacaa gccattaagt taaaccccgc tctcagcgag gctcatcatg agttggcttt
    10261 cgcattttat agactaggag agtacgagcg gtcgattgca gcagcagaaa atgcaatcaa
    10321 agttcgctct gataatccgg acacttatag gattctcggt tggtccttga ccggaatgcg
    10381 acgatggaaa gaggcaatgg aagcttttga gcattcgctt caattagatc cagattcttc
    10441 cgcaacttat tactcgctcg gaatggcagc caaagggaat gccgaaatcg attcagcact
    10501 tgaggcgttc aaagaagcca ttagcttgaa gcccgattat gcagcggctc atttcgagtt
    10561 gggacaactc tatttggatc ttggagaaac ccagatggct acgcaggagt acgagactct
    10621 aatcccactt aacccaaagc tggctcaacg gctcgccaag cggttgggtc tcgaataaag
    10681 gtaaggtctt gagctctctt cactgttatt aagtaacaat agaagcagcg tccaaccacg
    10741 ctcgactttc ccactcagca gcgtatacga cagggtttcc ttctcgactg taaaatgcct
    10801 tattaaacaa acttaaccac agtgcttaag catgacgaag ttaactcaaa ttgaaaatgc
    10861 tttgctagcg agcaacgaag cccgattcca ggaattatgc gatcgctacc tgtaccgcga
    10921 gggatacaag caaattaacc ggataggcgc aaaggtaggt aaagagaaaa caactaaagg
    10981 gcggcctgac acactgatat ctctatccaa tgggagacac gtattcgttg agtccaccac
    11041 gacgcagaag gacctgttcg ataagttgat gagtgacgtt gagagttgtt ttgatgaaaa
    11101 ccaaacgggt gttcccgttg acaaaattga aagaatcatt ctctgtcaca attcaaagct
    11161 aacggctgct gaacaggaag ccttgactag tagatgtcag caagagggat gccttttgga
    11221 tattctgggt atcgcttctc tttcgtttgg cttgcttgaa aagcatcagg tgcttgcgaa
    11281 ggaataccta ggaatcgagc ttgacagcgg tcaaatttta tcgccggaag atttcatcga
    11341 gctatatcaa aagagcgcgt ttgccacacg tctggatcta aatttccata gtagaaaaga
    11401 agaagtcaaa gccgctttag aagcgcttga taaaaatgac ttgctgatta tcactggacc
    11461 agcaggagtt ggaaaatcgc gctttgctct tgagtatatt cacaagttta ttgagcagga
    11521 cgtctcgtat cagtcattct gtgtatttaa taaaagactt cctttgtatg aagatttgaa
    11581 ggtttatttt ggaaacgatg gtcattatct actcttcgtc gacgacgcta atcgtctatc
    11641 gcaactggac cccttactcc agttgctgca tgatcaacgg aaaaatcgca agattaaagt
    11701 aattctcacc gtacgtgact atgctctgcc tgttgtacgg aaaactgcgc gtgagtattc
    11761 ctcgaaagtg gagttttatc tttcaccatt aaagaacgaa gagatacgcg aaatcataga
    11821 ggccgaattt ggcattcgca atcattatta tctggatcga atctgcaaga tcgctcaggg
    11881 taatccccgc ctcgcagtta tggctgcgat gcttgcaaaa gagacaaata aattcgaaag
    11941 catatctgat gcgtcaaaac tctatgatga atacttctct tcgataaccg ccgactttag
    12001 tgaattagaa acggcaaagc tgttgcagat cggcggaatt cttgcattct ttggttcgct
    12061 tgatcgcact accgaacttt tcaagcaggt ggctgagagc ttcgggcttt cggaaagtga
    12121 attgtgggaa gggttggaac gacttcatgc gtctgagctt gtcgacgtgt acgacaccga
    12181 ggttgctaaa atttcggatc aagtactcgg tacgtatcta atttatgcgg ctttcttcaa
    12241 actcgaatct tctgactttt ccgtgctgct ctctgactat ctcgcaagtt atgagtacaa
    12301 gtttagagag gccgtctacc ccattctgga catctttaac caccagttcg tcttgcagcg
    12361 actgcaaaaa catatcgata tcaaatggaa ggccgtcgat ggagatgagt cccaactcct
    12421 gcgactcatg cgggtttttt ggtttgttaa gaagaccgag atactcgtgt acctcaaaga
    12481 caaaatagac tcgttgcctg cgacgaaagt tgatttcgct actctaactt ttgaacccgc
    12541 atcgcaaggc cttcccaaag accaatacct tgaagttctg gaagcattcc aatatgcagc
    12601 gggcgaagat tttcgaattg cattggagtt gatcctcgag tatttagaga agcaaccttc
    12661 actcctatcg cacgtgcttt acctcctgct tgaaagattc tcttttgacg tagactcgta
    12721 cgatcgtgcg taccgcacgc aaagagagct cgttgacgtg cttatccgaa aatcaaacga
    12781 tccagaaacc ggacggcttc accagcgtct cctaatacgg attgcgcaaa actacttgaa
    12841 aacgcaattc agatcacacc gatccgaagg gttaaatgtg ctaattcagg agtttcagct
    12901 ccttccgtcg ccagaactgc tccaattgag aaagtcgctc tggctgttca ttgcgaaagc
    12961 ctctcgtgag tcggcgacac gcgagttcgc ttttcaggtc atagttgacc atgcgaccag
    13021 atttcatgat accccagtca acaagccaac gaatcagatc gtcaagctag attcagaagt
    13081 gctgcttcca tttctgagct cgactctcga tccttccagc tatgcggatg tccttgtaat
    13141 gcagcaatac ctgcgtttta tcaatcagca caaggtccgt attgaaagca agcttcaaaa
    13201 gcagtttacg aacgagacct atcgtctctc gaagattctg ctccctgaat gggacatcga
    13261 ctttgacaag cggcgaacta acgcgattaa gaaagaggtt gccggttatt cgtaccgtga
    13321 ttatctaaag tttttggagc gctgtattga cattcagaag tacatcaagc gggacgtcga
    13381 tgcgtatcag cttatgagcg ccattgacaa ggtgttagta aacctttcgg aaatggacgc
    13441 gagtcttttt aaaaaggtct tcaggtctct acttcaaacg ggtaataagc tcggccacat
    13501 ctacggaacc gcgatcgtta aactgcggtt gagttattca agtccacggc atgcttacaa
    13561 tttgcttaaa cgatacgaat acagtctcaa ggagtcatgg ctgctggcct ttctttcgca
    13621 gcttggtcct gaacaggtga acgggtttta tttgaatgaa ctctattcgt tgtatcgaac
    13681 tgccgattta cgcttgttca ctgattttga ttttctggaa tcataccgac gaattgataa
    13741 ggacgtattc gtcaatgcag taaaaatcgt tttcgatcgc agccaaccta agcaatcagc
    13801 caatttccat taccttttta atccgcatac ttatgtatcc aaaaacctga aaaacctatt
    13861 cgcggagaat atagaattgc tagaagagct ttatctgcat caatccgcga tcgaccgtct
    13921 tgtgaattat cgcggcttgg tacttaaaaa aatcgttgaa ctcgacccgg catttctaac
    13981 gaagtacctt cagtggttgt acggagaaca ggattatgtc tcagatccta tggatggtgc
    14041 gcggtacata gcattatgga cactagacaa ctaccaaacc gttattgcag aagccgttga
    14101 gtttgtcttc gagaaagaaa agtccgatta cgcccgttct ttcaattaca taaacgcatt
    14161 ttttagacag gatgacgaga gtttcagttc ggagtgtaag ccctccgtca ctgagcggat
    14221 ggagacattc atttcagagt atatacagac gcatcatcaa gatcgtcagc gaatgatttt
    14281 tatctttgac gttattacgg agtgttttcc agccaaacgc gttcgcttct taaagctctt
    14341 cctttcacta aatcaggact acgaaacttt tgaacgtttg gccatacaac ccaaccgttg
    14401 gggtggtgag ggaagtctcg ttcctgtttt cgaaaagaag atcgagttct tgcagtcgat
    14461 tctaccgctt gtttcgtcag tcaagtttct caaacacaag ctgcacgtca ataacttgat
    14521 cctagagtgg aaagaacgca tagagattga gaacaaaaaa caatttatag gatacttctg
    14581 aggagttagc cttaaagtcc gtgtgatgtg ctgtttacaa tctcacgtgc agcaaacctg
    14641 ggggacttcg tgagcaacag agccaatgcc gaccgacttc catctggtcg ggaattgttt
    14701 aaagtgatcg aggttgttga ttccgaagcg tggaacaact tagtaaggat ttcagacgcc
    14761 gttacgattt ttcatattac gtcatggcaa gactgttctc cgcacgcatt cattcgattc
    14821 ggcacttatc aaggagatcg gcttcacgct ggcgcgttgg tcgaggttaa cgagttcggc
    14881 gcggacaagt gaaaagtgtt ggtccatgca gtgtccagtc attgctcctg gacgatttct
    14941 tcttaatcaa tctcttgagg agttctaaag gtatgcagtt cgacataata ccctgtgggt
    15001 aagttggcat cccgaaccaa aatgcccctt taattgtggg aaactgtagt gggttgtggc
    15061 cttgtttttt tatagcagag ccaccgctta atgctgtcat gtcatgatcg aaataacatc
    15121 attatcgcag caggtgttgc cattgcgtga accgtatcgt tgctcagctt ttgtcgttcg
    15181 agggggaagt cgtttatgac cggtgtactt atgaagaatt cacatcagaa atagatttgg
    15241 ctcaacgcat ggaaaagaac tcagcatttc tgtctaatta cgtccacgtc aaatccattg
    15301 gcaagggctt cttcgcaact gtcacgcatt acaaagaaaa aagcactggc cgcgacgttg
    15361 cagtaaaaaa gctcaaggat gagcaatacg cttaccgatt tgaacgagaa atcaaaataa
    15421 tgcaggctct tgatgggcat ccgaacatca ttaaattgat cgatttcgag tgtgtcagcg
    15481 gttcgtatca acatattacg tcgaaggctg aaaccaatct ctacaaccat ataaagaaga
    15541 gaaacgctac gcttacgctt gaacagcgaa tccggatctt tgatcaagtg ctcgcagcgg
    15601 tcagttatgc tcattcaaag aagattttgc accgtgacat tgcaccaaat aacgtgttga
    15661 ttttcgatag tgaacacatt gaggtatgcg acttcgggat gggtaaggac ctcagcacct
    15721 tatcaaatta cactacctcg atagttgcgc agtatggtca agtgtattat gtggctcccg
    15781 agcagcggga taaattaaac gccgcctcag aaagaagtga catttactca ctggggaaat
    15841 tgcttaattt tattcttact gggcgggatc cagacatcat tcacccgtcg gacttcaatt
    15901 caatcatcgc caaggcgact cagtacgaac caaacgacag gtatcaaaat attcttgagt
    15961 tcgaaaccgc ttacaatcgc ctcaagtcgc tccttctttt agatacaaca tcaacgctgc
    16021 cgaaaacact gctcgacttc aaaggcgaca acggaagtat cgattggcaa gaattccata
    16081 agttggcggc acgttcggat cacgaagggc acccatattc tgattacgtg gacccaatag
    16141 tgcaagtgct ttcgcggcca ggaaatctgc agcagtacta cgaagccgta ggcgattcgt
    16201 ttgtagattt tctaacgaca ttcattggga aattgaacga actcgctgga atgacaggat
    16261 ggccttttag tgaaaccaaa acgtttgcga ggttcttaag agatatattc tttggtgttc
    16321 gcgaaccagc actcaagctg gtttgtctga agcatatatg ggatctcgct tatgaacaag
    16381 atcagtgggg agcgcagtcc atcatggagg ggctattggc agacgatcat attcccatag
    16441 acattcagac ctcgtttgct gttcatattc tggattcgtc aataggaagt ctgccagatt
    16501 cttctaagtc cacaattcca agaatcatta aacaggcact ggcccaaaag gctaatgaac
    16561 ttgacgctca gcgaagactt gcgagcctcc gagcagtgct gaaggcgctg aatgacaacg
    16621 aacgtgattt gctcaaactg tttgcgagta ccagatcaga atcagatgag tttgaccatc
    16681 cctttctgcc atataatgtg tatgattctg tcaacaacct aattagtaac ggcgtgttga
    16741 cgcgagagca aaaagcaggg ttaggcgaac acgagcagtt aatctcgcta tctcccgaag
    16801 ccgtaaatct tgttgagacg ttgattattg aagacaaagt tcggcgcgaa tcgattgtct
    16861 tagaccgtcg aaacattgcg gcatctggcg ctagtggtag cggtgcaccg cctcacttaa
    16921 atcatcgctg atttcgaaac aagtcgtttc taccctcagc cttcatccaa tctaattctt
    16981 ccgagaagtc ttccgttctg ttcgaaagac cgtcttcgaa ctatgccaac ttttcctgcg
    17041 gctgagcctc tgctgcgagt gtctcgcatt ccattgcgaa acttttcagg tgataaggat
    17101 cagaatgact tacagcacgc ccgccgatat ataccgtcac ctcatgaaac ttatttcgag
    17161 aagattttca gttgaggtac cgaatgcttg gcgaagattt ttagtgaggc aattcgatta
    17221 attgacaggg gattgatgaa cacgtagatt tctttactga gttctattcg cttttcactt
    17281 ggcgaactcg agttcgcttg gcctcgcaca agtaaccgca agagaatact ttctcttgtt
    17341 acctctaacc agcattcacg tattcatctc aaacgatgag taattcgctc tcgctcacaa
    17401 caatgactca accaaaactg aatccactat ctcggtctgc catcgtattc gttatcttga
    17461 tgctcttcag agtcacgagt ttggcgcagt tccaaaccgc gcagatctct ggcttcatta
    17521 gcgacaaaca cggagcgact ctcctcgggg ccgctgtaaa gatcgtgaat gccgatacga
    17581 gagaggaaca ccagactctc actgataaaa ccggctattt catcttcact aatctcatgc
    17641 caggcaaata cgagatcgcg gttgaaataa ccggattcca aaaattcctt caaggcgaag
    17701 tcaatgttga cgcgcattca aaggttcgtg tcgacgcgtc gttggaaccg aagggtgtca
    17761 atgaaacggt aaacgtcgtc gatgcacggc ctaacataca acgcgacaca gcggtgatcg
    17821 gtaagactat cgagcggcga gacattcagg atttagctgt taacggacgc aatccaatca
    17881 acttcgtatt gttggcgccc ggcgtgcgcg gagggccttt taactccttt aatccagacg
    17941 atctcggcgc tattggattc tcgatcaatg gcagtcgcct cgaagaaaat ttgataactg
    18001 ttgacggagc gattgctatt cgcacgcgcg cttccggcgc gataatcggt attccgaacg
    18061 ccgactccgt gcaggaattg caagtattta cgtcaaacta cttaccggag tatggccgct
    18121 cttcgggcgg ccagatcaga tttacaacgc gagtgggtgg tcgcgatttc cacgggagcg
    18181 tttttgagtt ctttcgaaat tcggcgctcg acgcaaatag ctggggacgg aacgcgagca
    18241 acaacttgga tattgcgcgt aagcccgcgc cattcacttt caatcaattc ggctatgaca
    18301 ttggtgggcc agtttacgtg ccccgtagat tcaaggtgga tcgtgagaag ctgtttttct
    18361 tttgggctca ggaatggatt gtctggcggc ggttcgatac cagtactcaa acagtcccaa
    18421 cacaaaaaat gcgaactggt gattttagcg agttactaaa tcccaacaat cctttcttcg
    18481 ccagagctgt ctttattcgc gatcccttaa aagccggtga atgcagcgca aacgatcaga
    18541 cttcgtgctt tcccggcaat gttattccat tggaacgcct gagtcccaac ggcgtcgcat
    18601 tgttgaaaac gtacccacta ccaacgcccg gtttccagcg tggtgtttcg aactacatcg
    18661 cacaaagtcc caatccgcgc gatactcaaa aggatacgct caggatcgac tacattatcg
    18721 gcagcaataa ccaactgtct tttcgcggtt cactattcaa ctgggactca gtggacgctt
    18781 ttcgcggttc ttttccactg gcgcggacgg attggagacg gcccaacaga tccgcggcgc
    18841 tcagttggac cagtcaactc tcaccaagtt tgattaatga actaactctc agcgcctcgg
    18901 tagatcgtgt gttcatcaac gtcttcaaca acggcctgta ccagcgcgaa cagtatggca
    18961 tcaattaccc gtacctgttt ccgggtaaag aaattgaaaa caaaatcccg acggtcacga
    19021 tctcaaactt tggcacagtg gacggtgggc cttatccgtc gaagtcttcg gggccgattt
    19081 atacgttgtc aaataacatc acgagggtga cgggccagca ctcagttaag accggagtat
    19141 ttatcgaata ctcaggcgaa aatgacttcg atcaaatcaa cgtgcaaccg gtgccgggtg
    19201 ggacgaacaa tcaaaacggc aggttcgagt tcactaatgc acgaaccaac gggactggac
    19261 ttgcagtcgc aaacgccgct cttggcctat tcactaacta cggagagatc ggtcaacgct
    19321 cttacacacc gtggcgcgct tggtccacgg atctattcat tcaggacacg tggcgggcac
    19381 gtcgtaacct cacactggaa tacggaacgc gctatagcta ctggcaacca tggcgtagtt
    19441 tgtggggaaa tatcgcgatg ttcgatccgg ccttttacga tcgagcgaga gccgcggcga
    19501 ttgatagtgt aggcgggttc ctaattaatc cgacgtttga gagtcgcttc aatggaatag
    19561 tactgcccgg tcgcagttgg ccggaagcag ctcttggtcg cgtcaatgcg gcaagcgatc
    19621 cacgattcca aagtcttttt cacggactgc cggagggctt cagcgccact cataagaacg
    19681 tcttcgaacc gcgcgcaggt attgcctact cgtttcacaa taagactgta gtacgtgctg
    19741 ctgccggcat tttccataat cgcgtaacgc tcaacgactc aaccctgctc ggtggcaacg
    19801 cgccgatcca gttcatgacg ggcgtaactg atggtcttgc ggatgctccg ggtggagcga
    19861 cgatgagaac gtttccgcta gtcgtaacaa tgcaggatcc ggagttcaaa cacccgacct
    19921 cataccaata taacttcacg attcaacgag agcttccctt taaaacgaca atcgatattg
    19981 cgtacgtggg acgaatcggg ttgtatttgc agcgcgagcg caacatcaat caactccttc
    20041 cgggaacgcg acaagcgaat cccggagtta acacggatgc actgcggccg ttcaagggat
    20101 ttggagtgat acggctgtcc gagaatgcgg gacgttccga ttacaacggg ttgcagttga
    20161 gcgtccggcg tcggtttcag aggggcgtta gcttcggagc cacttacacg ttttcaaaac
    20221 tgacggatga tgcgagtaac cgaagagagc ggctgttcaa cgcatacgat gcgcatagct
    20281 ttcgcggaac ctcgagcgac gaccgaacac atgttttcaa cgttcactat gtttatgaga
    20341 tcccgttttg gcgagagcag aacacgttag ttaagaaagc gctgggaggc tggcaggtct
    20401 cgggagtggg gctcgtcgtc tccggacaac cactaacagt ttgtcgtggc gatgacgacg
    20461 ctggagttgg agacagtgac tgccagccgt gggatttggt tggggatact cacgttgagt
    20521 cgccttcatt ctctcgcggc cgcaccgtgg accaaaattt ttggttcaat ccaactgcgt
    20581 tcaggcgccc tgcaccgggt acgttcggca atgcggggcg gaatattctg cgaggtccaa
    20641 cttttcagag ttgggacatt gcgatctcaa agacgttttc aattgatgaa ggaaagcggc
    20701 tccagttccg gtcggagttt ttcaactttc ccaaccatcc taatttggca aacccagaca
    20761 cgaatccgac gagccccaac ttcggacgcg tgctggggaa ggcgggggag cgaaatattc
    20821 agtttgggtt gaagttttat ttctaattta agtttatccg cgcggccgaa agggtgcggc
    20881 ataacgaact cgcaatgtgc gggcccaatc gtcgatcttt cccttgtatc ccgtttgaaa
    20941 acgacccaac ccaccaaccc ggacttctat tactttgttc ttctggtcta ttgcgacgcc
    21001 ctcttgtcga aaagtccaac ctctttcggt ccaccgacag aataaaccaa atggatttcg
    21061 gcattcttcg gcagcgagaa cagaactacc cagtcgggcg gattaaatct gcttttttcc
    21121 ttctcctcgc gccaggtact tggatatgca atcatttcgc tccgctgagc aaaaacaggt
    21181 gcacaggcta caagcaccaa gctgattaaa agtaatgtac gcactctatt catgcgctct
    21241 cctttgtaga caaaataaac aagcaatgtg tgtactttac tactatgatc ttcgaggtcc
    21301 agaaggaccg cgcggttcaa acggagcgta atacactaat cggagtgagt tggccaggct
    21361 tgacgagaac ttttgtaatt tccagtttat gtgcgcggta ccctgtaata actttcacat
    21421 atctttgacc acgtgcataa aaaattaaag tccttcgtgc tttgtctaag caccgcactt
    21481 tcactctctc ctcgcgcctc tactacgatg tctgttccat tgctgggata agtaagatag
    21541 acaacccaat ctataccatc ttttttgtgg ctgatgagag cgagtgcggc ggcctcaacg
    21601 cagtcgcact catccctttg tcgttcagcg actaccttac tttcccgcac aaacgattat
    21661 agcgtgcgga attgatctag tcggcgatat atagcttgtg ccaccgatta tcaaaccagt
    21721 actacctcct ccatctaaca tcatctttga acccgtgcca aacccactca gtacgcttac
    21781 tgcacctgct tgggttgcgt aattgctcga aaagaaaact acagtttcag aatggccgtt
    21841 attatcatca tcgcgaacgc caacaaacgt ccggccaata taactgctag ccgacttatt
    21901 ggccgttgga tcaagcccac cgacaacgtc cggaattcca gcatcgaagg ttgcagtgga
    21961 gtaaggctga atgctcgaac tcccgtaaaa cgaatcgaat gcgaatgtcc gaacaagtcc
    22021 gggatactca ttaatcccat atccataact catccgccac cagtcagcct tcaacccgaa
    22081 tgcaatcggg gtaaacggcg aatcgtttgt cgagaagaag gtgccgttga ttgccactct
    22141 tgctgtgcgg gaaccggtat tctgagcaac ggcgtcgttc caaaacctac tcagtgttct
    22201 gcgctcaatg ttttgtccgg agacgcttcc ggtaaagtta cggatcgtgg cataccgaag
    22261 atccacaacc gtaacgaagt cgggttgccc tccaacgtaa tttttgcgat atacctttac
    22321 tccggtatcg gaaaggatga gttgaaatcc tgatggtacg ggtactgcgg ctttcacttg
    22381 aggggaatca agaagactgt tgaccaaaag aacaggcagt attaagagaa aacttaaagc
    22441 ttttcgtttt actacctcga gtgaattgat tgacatcatt tacctccaat ttgtagaaag
    22501 ggtttccaca tccagctttc gtggaatgca gcgaagttgc agcgtgactc ctcatggcct
    22561 ccgaggaccg gacgcttgct tgcgccaaag atagagatat tggtaatcct tgccagacag
    22621 gtggcttttg aaaccaatgc agacgcgcgt tgttggagtt gtgagacctg acgcggccag
    22681 gctgcctccc atcgctacaa ctacatccgg catattcgac caccaccgga tgtcatcggg
    22741 attaccccta cctgcgtttg gcgtattgaa gattaagatc ctgtccggca acagcctgtc
    22801 cttccagcca cctccgcatt cccgtgcatc gcgcggcttt gtgatcgata caggaaaagc
    22861 catgttggta ttgccctttt ccctctcaac ccactgatcg ccagaccctt gagcattagc
    22921 ggacacaaaa gctcctataa atacggtcag cgccagtagg catgaaaaaa ttttaactag
    22981 ttgcatttgt gagtctcctc gtaaagatgg gttacggtaa gtcgtgaacg aaacctaact
    23041 agtgcggtcc aattcaatca acggaagctg atcgactgaa tcactcgact attctcttgt
    23101 aagtacagat tagcgttcgg tccggaggtc acgttcacca catagaacag gttttctgca
    23161 ctatgctggc aagtgtaaat ctcaacgttt tctgtgtagc cactctgagg tgatgggcca
    23221 gccaggcgac tggtgatgca aggaacgtta ttgatcacgc tactttcgga ccggccgatg
    23281 cgtttgaggt atttattcgc cgacagtata cctcgaacat accggtctgt gcccgattca
    23341 aagcttgcgc cgttcagatc cgctagccca aaaattacgc cattagccag attgccgttt
    23401 gtgtactcgc tctgtggcgc aaccgttatc gacgaccgct cctcgaaggc tctccaccct
    23461 gatggagtga aataagagaa actcggacgt ggagctaacg tatagctcgg ttcggctgaa
    23521 cgacccgtac gattcgattc aatgggagta cgaccgcgtc cgagtccctt ttcagtaggc
    23581 gtttcagttt gggtaggagt gtcgctaccc ggtaggcact tacctgaatg ttcgcagact
    23641 actcttgcga ttgctccaat caaatccctc acaagcgttt gtcctgctga tggcgctttt
    23701 ggcccttcag tttcggtcgg ggtaggctct cgcggaacgg ttacattctg agcgaaggtg
    23761 gttacttgac caccaaaaca aaacgcaata acagcaatca agctcaatcg tgtgtgcact
    23821 ttcatcgtta actcctcgcg tggggtgagt tcaaaagtct tctcataaat acccgaacgt
    23881 gttccgggcc acgggttcgg tcatgaggct ccaacccatc acactggtcg ggtatttatg
    23941 ggaggactta ataaaacaat gcgttcgcgc aaaagttggt ctattcttag gtggttcact
    24001 ctcgcaagcg tgtaatcacc tccccggtgg tatctcagtg aattgtgaac tggaaaatcc
    24061 ttctactatt actaacgtcg ttctcgtcct gaacttctaa ttggtgtgat aatgtcggaa
    24121 agtgaaccgg aaaagacctt cgcctcaaac gaaattaccc gtcttctcaa cgcgtgggcc
    24181 ggcggtcgtc actcagcggc tgacgaactg tttccgttga tccaacgcga attgcacaag
    24241 atcgcgaaag aacatatgcg gcgccaggat ccaggccata tacttcaaac aacggcgttg
    24301 gttaacgaag cttacgttag gctggccggc agcaaggata aaacgtggaa agaccgtaac
    24361 catttttacg cggtggcttc gatggcgatg cgacatgtgc tcgtcgacta cgcccggaaa
    24421 gaactccgag acaaacgagg ttcaggtaag tctgacgtac cacttgatga tgccactgaa
    24481 attccaaatg aacccagctc tatgctgatc gcattagacg aagcgcttcg ctcatttgcc
    24541 aaactggatc cgcgtgccgc acaggtagta gagctccgct actttggcgg cctgagtatt
    24601 aaagaaactg cgagtgttct gggtgtgtca cctgccaccg tatccaacga ttgggcagca
    24661 gctcaacttt ggttgacacg ggaactgagt gaaaacaaga aatgaatgcc gatcaatggc
    24721 agcgcgtcaa agaattgtgt gttgcggctt taacattcgc gccacccaag cgagaagcct
    24781 atctggagca ggagtgtgac ggcgacttac gactccttga tgaagttcgt tcgctcctcg
    24841 ctgtgcagag taaagcctcg aactgtttag gagaactgcc gcgcgagttt gtagcagcgg
    24901 catttgagaa ccacgtctcg ttagtcggtc gaaaaattgg caactatgac gtacggacac
    24961 tgctcggccg tggtggtatg ggggaagtat atctggccga agatcgtgac ctgcgccgta
    25021 aagttgcttt gaaagttttg cctgaggaac tcaccaggga cacacacccg cttcagcgtt
    25081 tcaaacagga agctcgcgcc gcttcagggc tgaaacatcg caacatcatc acgatctatt
    25141 cagttggaca ggaagatgga atctatttta ttgccgctga gtatgtgaat ggtttgactc
    25201 tgcgccagcg cctgatgagt tccggccggc ttgaattgaa agatgcaatt gagatcggga
    25261 tacaaatagg ctcggggtta gaagccgcac ataacgccgg catcgtgcat cgggatgtca
    25321 aaccagagaa catcatgttt gagtctgatg ggacagcgaa gctactggac tttggcattg
    25381 ctaagctgac cgaaactctt ggcccggaaa atgtttcgtc cactcagccg ttcaagacgg
    25441 agcaagggat catcttaggg actccagctt acatgtcgcc tgagcaggcg cgccatcaat
    25501 cgcttgacca ccgtagcgac ctcttcagtc taggtgtggt gttgtacgaa atgatcaccg
    25561 ggaatgtacc gttctcagga ggaactccgt atgaccaaca ggctgccgtg ctcgttggcg
    25621 agcccacgcc gatcaatcaa catctggata aattcgtccc tgagttgcag tgggttctga
    25681 gtaaggcgtt aacaaaggag cccggacggc gatatcaaac cgctaccgac ttcctgatcg
    25741 atctacagat gcttcaccag cgattacagg atgaagatgt agacaataag aagtctgatg
    25801 taacggacgc aaccagttct cgcgttactg tgatcgcaga caatagaccg ttgtggaaac
    25861 gtcgttggac gctcacggca attgttctgt taggcttggt cgctgcactt gccgcagtaa
    25921 tgatttcgac gagagcgggc caacggcttt tcggattaat agggccgcca cccgtgatcg
    25981 aatcaattgt tccaacgcgt cctattgacg tgatcgggaa tcagcctatc gaattgcgtg
    26041 gtcgtaattt cgaggatgga ctaaaactca aggttggttt tccaaacggc ggctcaggag
    26101 agctttccgg agcacagatt caaaaacaag aaggcaacag cacgacactt aacatactaa
    26161 tcgaccttaa tgggaatccg ggtatctaca ctcttcaact gttaaatcct gatggtcagg
    26221 tctcgttacc cttcaatttt gaagcgcgtc atgaaacgca aagaccctcg atcaattgga
    26281 ttgagccgac cgttgtaaag aagagcggag gcgaacaggt cattgccgtg tatggcagta
    26341 actttcaaca aggagtatcg gtcgacgtcg tacaaccaaa tggtgagacg tcacagcttt
    26401 caggtcggca gatcgcgaac cagacgccga attcgtttca cgggttgttc ttcttttacc
    26461 tgccaggaaa gtattcgatt agggttataa atcctagtgg aggaaagtca gagtactacg
    26521 acatcgtagt tcaataatct ccgaactatg attcctatca tgcgcagcag atgaccttgc
    26581 ttatctggcg gttcaccctt gctgcatttt attgataggc tcagattggt tctatggact
    26641 gttgccgaaa cgctcagcga ggagaaacga gtcgtttctg cctttcagca ttaatccaac
    26701 ctaattcttg cgacaagtgt tccgcccttt caaaagacta tcttcgaact atgccaacct
    26761 tcccgcggct gaacctctgc tgcgagtgtc acgcattccc ttgcgaaact tttcgggcga
    26821 cagggaccta ttgcgccggt accaataagt aagttgggat caactacaac gaaagcggtg
    26881 gcgggattga gggattgtcc aacctaccga gcacggatct aacggtatag tctgctgttt
    26941 tttgcagtag ttgctgattg gttccatagt cagtttctat catattggtt ggtcactcat
    27001 cccaataatt gctgtacaac cgagcaaagt ataacctttc cgaaggacct cgttccggca
    27061 ctgcagatcg acttgtggat cgtcctgtaa tattgagtgg aacaaccaat caaccattcg
    27121 accaaacgtt ttgtctaagc agcttgggga gttctatcta tgtgaacgat cggtatggtg
    27181 gtatgtcaaa gattaagagc tcaaaatcta aatcggagaa cactcttaaa agagagatac
    27241 gcgctttgcg ttcaactcta aatcgcgtct gggcaacatt ggtcgacatt gatgaggcta
    27301 ctccattgga ggaggcgctg gatgctatag acgaagcgtg cgaaataatg gccgcggatt
    27361 ggccaaacgt ttttgagata actgatgagg tcagtccaaa agtacaacaa caaatcgact
    27421 tggaaaacca ggtaaataag ctagcagagg aagcacagcg gactgacaat tggggtacga
    27481 gggcagttct ctttagaagg ttcctcgctc tcgctgctgg tctgagcgac gagcaatttg
    27541 agcaacaaat tcagcaatta acaccagatg agtacaggga tttagcgctt cgaaatctat
    27601 ggatgatcgg agcaatcacc gcccctactc cgatcgacta taaggatcag acagatcggc
    27661 ttatacgtac ccttaaagaa tttaagaagt cactccctcg aaagcatggt cccgagccga
    27721 gtcctgttat tttggaagct gttcgaatac gggatactga gaacaaatca tacaaagaaa
    27781 tctacaatcg tttgattccg atatttgggg aggaaatgag aaatctgacg ccagtaacgc
    27841 tcagcgcgcg cgttcgaact cgacgatcgc gcttgagaag atccaggaac tccgatcagt
    27901 cgtgatttgg accgtcacag ttagctgctc caaacaatca cgcgtcacag tcaatttcac
    27961 taaatgtgac tcttgcgcag cgtgggcctc agagctatgg tgggccaatt gatacgcagg
    28021 aggaacgcaa gcaatggctc agatatttat ttcgcacagt cgaagagatg aacaaacgaa
    28081 gaactttttc tatcggactt tctcgggaac ggcagtgcta ccaatttgga tggagtatga
    28141 ggacgccgcg acgagttcta tagatcagac aatcgagcgc aggatccacg gatcaaacgc
    28201 agtgtttatc ctactcagcg agactgttga acgcattccg ttcaccaggg attggatact
    28261 gtgggagaac ggcaagataa ccaataaaga catttgggta ttcgaacctg aagattcttt
    28321 gggtaggatt aacgtggttc tgccccgctt ccaccactat gttcgtgatc ggcgaactaa
    28381 atcctggaga gactacatca attcgatagt tcgatcttat gacagttcga acctggtgag
    28441 tttggccggc accggcgccg gagttggagc aatactatcg gatgaagata gagcggctgg
    28501 cgctattgtt ggtggattgg ttgcaactat tattgaggct atcggacgac caaaagcccc
    28561 aacgggtttt cccgtcagtt gtttgaattg ctcctacaat tacgccgttc accttactcg
    28621 cggcgtcaat caattcagat gcgcacgttg caattgcata ctggaactcc gttcacaaga
    28681 gttcctatca gtcaatcgaa tagagtgggc tcattagctt atcggctaag accacagctt
    28741 gagagttttg tgtgtcgcgg attcatgctc gagtgtcaag caaccttttc agtgtgtgag
    28801 ctgggccgaa cgttggatcc agtttaagcg cttgcttggc gcactttaag gctttcgagc
    28861 gtcttccgca tttccaataa gctgttccaa gttccaccca ggcatgagga tgagatggtt
    28921 caatcttagt ggctcgctcg gctgctttta ctgcttcggg gtagcggcca agcccattga
    28981 gtatggcggc cttattaatc aaacctgaaa gattttgtga atccaatcgg aggctctttt
    29041 cgcaaagaga tagcgcctcc tccagtcgcg caggattccc ttcccggact agatgagctg
    29101 cgtagtttgc gaacgtctga gcagagttct caccaagcgc tagagatagc gtgtaggact
    29161 gctccgcgcg cgcgttgtcc ccaagtatcg aaagactgat cgcaaagtta ttccagccca
    29221 ccgggtttga cggctccaaa cttacagctc gcaaataaca atcagtcgcg cgacgatgtt
    29281 gtccaagttg agctaatgcc tttcccttgt tgttccactc cgccgccgtc ttggaactat
    29341 caagctcgca cgcgcagagg tcgtcattgc cgagcccttg taagagatca ttaaaggtcg
    29401 gtctcctgga aggatcttgg gccaaacact gggccaaaag gttacgaata gacactggaa
    29461 ttagcggatc atccagaggg ggaactttct gtcgtcgatc tagcgcgttg agtccacctc
    29521 gactcgtctt gcgctcaaac gggcattttc cggtcagcat ttcatatccg actactccag
    29581 cagcgtatac gtccgacttt tcagatcgtt ccgtaccctc ccagcactcg ggagccaaat
    29641 acaggaaagt tccaccagtc tgttttgaga atgggtcgtt aaaaccacga gcgcgagcca
    29701 atccaagatc cgtaacctta agttcgtggc ccctcgctac taaaaggttt tcaggtttca
    29761 aatcaccgtg aacgaatcct ggggcttgca gttgacaatg gaccattgcc ttacatagtc
    29821 cgctaaaaaa ccgcaaagca agaggcagac ttacgaggtc gtgatcgatc cattccttca
    29881 ccgtagtcac gccgagcggt ccgggcaaga ccgcctccat gaccagaacc aatcgagaag
    29941 gtgagagggc aatgacatcg aataattgga caatgtttgg gtgcccgcct aagtaggacc
    30001 aaatcctcgc ctcacttttt aacgcttcaa tctcaacatt acctgatgag gactcgatgg
    30061 ttttgcagac cacgaagtat ggcagcgacg agccaggaga ccattctgta tcgacgcata
    30121 gataagcagt gcccattccc ccgtgacgaa ggccatgaac ctgaaacctg ccggcgatcc
    30181 gtcgaaaata tttatctaag attgctttcg aaccacgtct aaagtcttct tcgaccgtat
    30241 ctgccgatcc cgaagcaagc ttggcaacag cttcgcgata tcctttttca tcaatcgaat
    30301 atggaccatc ccgtggtacc tgccatgcct ttcgtatata atcttgaggc tggggttgaa
    30361 gggcggctgt tatatcaaac cagaattgag tttcaagtcc gcatgaattg cattttgctt
    30421 gcacagattc aaacgactgc tcatcttgaa aatccaattc ccaccgcaat gcgctgtaat
    30481 cgccgccaca tgaacatctc tgttcagcga ggtaggtgtg ttcttcccaa gtgcttgaaa
    30541 ctgtaattgc ctgttccaca gacgcagttt acagcaagcc ggtcgtaaca ggcttttcta
    30601 ctacaagttg ccaagcaggc actcttccga tcactgcgag tatccctttg gtctaacctt
    30661 acggcgggtt acaagcgggc gacaatgtga cgatgttgat aatcaccatc aggcacccgt
    30721 agtttggagg taaacatcac taagggtggg aggactcgga catcatttcc cgcaattctg
    30781 ggatgtggta taagctgctt gtaacgcata caagtcctac gttcgaacta aactcaccag
    30841 caggacaaag gccgggtgcc agttccataa gagatgggtc ccactcatat tacttgtcag
    30901 cccaaaggag ccccattcga gttctgaacg caacctcatg cgcataattc cccaacaact
    30961 gagtttcgac gctaaaaatg aagcggtttg tctgcatctg tcgcaaatat caacatcact
    31021 caatcaaacc aggaagaaaa tcaagaatcc gaccgagcaa tggcaattga tagaagcgat
    31081 tctcaggtcg gtttcatcaa cagccgatcc ttcacatgta aattttctga ttcttcctga
    31141 aatcgctgtg ccgtaccact atgtcatgaa tctctttgag attgtgtctg agacttttcc
    31201 aaagaactcg ataacaattg ctggcattga attgtccacc gtcgatgagt gcaaaaattt
    31261 agccagccga ctcaagagtg ttgaagcctc aacgttactg caaacagcag aaagtgaacg
    31321 tctggttaac caatgcttaa taataacgaa ggaacgaaac aaatgcaggc cgctcctaca
    31381 actaaagatg actccgtcca agttcgaggg tggagttgtt acagccgaaa gcctattgtc
    31441 aagtgattac ctttattatt ttcaaaccaa tgggccaata ggtgtaagct ttgtcacttt
    31501 gatatgcagt gactttttta accgaccgga cggagcgcta agtaagatta ttgacgaagt
    31561 tgatttccag atacttaaac gaggtcgggc tttggatttc ctgttcaaca tccaacacaa
    31621 ccgatcccct gacaacgaat tgttcttgca tggtctaaga aggatctatg acgatgggta
    31681 caaagcccac ggtgatctat gcacgatttt ccttaatgcg gcattggatg gtgacaaagg
    31741 cggtcgcagt aagattcttt tttacaaggg caacttgctt cctgaaagac aaccgataaa
    31801 gcaacttgat gcgccagtgg ttggatacga attaagcggg gcgcaaacct tgtcgtctgt
    31861 gcatttcaag agacttccgc gatcttggga caaccgacgt gacacttatc ctgtggactt
    31921 tacgtgttac aaattcagtg agaaacaatg gacgacgaac aaggattcaa agatcgttta
    31981 ccttgctccg atgcagaccg aaccatacct cgatcatcgg agctacaagg agctagctga
    32041 aagattatcg gtactaggtc gtttcgacga ttccattcat tgggctaaga agagactatc
    32101 tcactctgag gaaagaaaag actttcacga ggctgcttta gctgctctct ttatggcaat
    32161 ccaatttcgc cacaaaggag agctaacaga agctttgaag cactacgccc acgctgaagc
    32221 ccacatattg tcattaacaa agctaacccc cggtgccgcc ttaactcatt ggcgaatcca
    32281 gctcggtcgg atcatggttg aagactttat gatcaacggc cgatgcaagc aggcacatga
    32341 taactaccaa acgtttggac gggaaattga tgaatacttg agtgctcagg gtagttctga
    32401 tcacgagtta ggatggcgcc gagagatcga acaaatcaag ctctataaga ttcacctgag
    32461 taggcaatgc gccgaaatgc agaggcttct aggcaggtat aaaagagcaa gatcgttgtt
    32521 cgatgctgcc tacaaacagt attcgtatgc ttacggagaa gaaaaagctt attctgcact
    32581 gggatgcgcc gatagcttgc gaatgatggg acgatatagc gaagccatcg ataagtatga
    32641 agaggtgcgg gaattcgcca caataaaggg tcagagaaga ttattagctc gtgtgctcag
    32701 gaacagggcg gagatactgc gcattgaaga tacctgggac gtgctcaata cgacactcga
    32761 agaactgact ggactatcga atgaaaccga ttatcgtttt gggaagatat atataccgct
    32821 tatcagaggc gctgcgcggc tgcgaatcga tccgaagcaa gcaatcgaaa tattctcaca
    32881 aattgaaagc ctagtgtccc gcttcagtca aaattacttg aaggttgaat ttgctcacta
    32941 tcttttcggg attgctgagg caagaagact ctgcggtgag cgagaatctc agcaattcta
    33001 cgaaagagct cataagcttt ataaagaaac aggtgttctt tggggcatca tacggaccag
    33061 cgttgggtta agtttggtaa gtaacagctt gaatggcttc gagcaatctc tgaaagggtt
    33121 aaccatatct gatcccttag atcgtagttt tatcgaaaga gcacagcaag gcaaactcga
    33181 taagaaagat atcttgcttc ttaatattcc ctaaacaatg gcacaaataa tccttgcaga
    33241 gttcgaaaac gtcaaaggca atatccaatc ggatcttccg tacgatttgg ctttggcaga
    33301 gtgcttaagt tcaaaaagcg atgcgctttc gaaggccgat ctaaacgaaa cgtcatatgc
    33361 agtaaagggg gtagctgagt gctaccggcg tctagggcta acagctaagg caatccggga
    33421 atacaaaact gcgttaagcc tatttcgcga gacggcaaat gtacgaggaa tcgcctggtc
    33481 gaaatggggc ataggaatat gtgaaagaca acagggggat tacctacaat caatccatac
    33541 acttcgcaaa gctaagagac atgctctgct tagcaatgat aatagatgtg tggcctatac
    33601 ctgtgccgga atagcagaag cggtccgaat cctcgggcat tacaaggttg cactgttgca
    33661 gcactacact gtactagagc gatttaaatc acttcgagac cttagaggaa tagtatgggc
    33721 attagaaggt atcgctcaga tacacaaaaa cagtggtaga tatcacgatg cattgagact
    33781 ttttgacact tcaaaaaaga tagcgtccga aacaattgat tttcgcggcc ttggctacgc
    33841 attaaaaggc tcgggtgagt gcttgagttt acttggatta tatgagccag ccgttaacga
    33901 tttggcttcg gcagtgcgac tctttgagtt aatcaatttc agattaggct tagcttatgc
    33961 ttacaaaacg cttggtgacg cgtatctacg atctgattcg ttagctgaag cccacgcgaa
    34021 ctatagcaac gcgatgccac tatttagaca gattaatgag gacagaggga tcgcgttttt
    34081 acggaagagc atcggtgact tcaacttgcg cgtcggttcg ctcgacgagg cgtatgacaa
    34141 ctacctaaat tcccagactt tctttcgaga aaaaggcgta aggtacggtg ttcatatatc
    34201 aactgttgcc ctcaatggag ctagtacgaa tctgagtaga agctactccg tcccatgtga
    34261 cgccgcgaaa cgaaagcgct caacgagctc atagtgtgca aacaaagaaa acgaggcgat
    34321 acggaaacgc actcagacgg tatggtattg cagaaaacgc taacaaaaag ataggtggta
    34381 gttattgaga cagcctcttt aagtctgcaa gaacttatct ttatcggttt gattccgatc
    34441 cccgctccat gcctttaatc ctcctggaag agaacaaaca tcctgagatg cataattacc
    34501 tgctcagtag tcttaagcct gccgcttgtc caagctggcc gccatagcat tactaaagtt
    34561 acccgcataa ttaactcccc atagataagc cgcagcagac acaatcgcga gaacaatgcc
    34621 gaagaaaaca agatttgcag aagtgctggt ttgtgcccgt tgccgggaag ctaaatttcc
    34681 cgtaatcgca tcgtcagaaa tatttgaaag agattgcgca ttagtacgtt gtaaatcggc
    34741 ggcgcagtat ttgcagaact ttgcctctgc gtcaacggat ttaccacaat tcgggcagtt
    34801 catgacgtca gttgcggaaa tccgagcggc gcaggacgaa caatattccg cgtcatcatc
    34861 tactcgcgcg ctgcacttcg gacaaaagac ttgcggacca gcaatttcag agatatctgc
    34921 tccgcaagtc ggacaaatca tatctgcgct cgcaacctcg gcgccgcaga tctggacgtc
    34981 gttcgtaccc aactgcttac agagatagtt acaaagtttc gaaataagcg tgtcgtgatc
    35041 attcatgccg catcgggtca aggtaaaacg gcccttagtt atcgatacct tcatgaatac
    35101 tttccgcaat cttggcgctt tcaagttagt tcggtctttg atcgtgaaca ggcactcaga
    35161 gtcgccctcg cattgaaagc tcatgccgac gcgctcggcg tcccaatggt tatatatctt
    35221 gatgtgtcac ctcaagatcg tgattggcct gagttggtga gaagcctatc aacgcaccac
    35281 aacctgcaag tgttagtcag cattcgcgag gaagattgga agcgcgctgg catcctggtg
    35341 atgggatttg aattcgagac tgtcgaactc tcgttcaaga gaaacgaagc ggtgacgatc
    35401 tacgactcgt tagtccagaa acatccgccg gcaaacgttc taaatgctga agaggcatgg
    35461 gaaaggtttg gcggtgctgg tccattgatg gagtttatct accttgttac gcagggagac
    35521 tctttacgca cgaaattgtc acagcagatc acttacctca aaaacgaggt tcgagaaaaa
    35581 agattggatg tctccgaaat cgagttcctg cgtctggtgt ctatagcttc agcatctggt
    35641 gccaggctac gggtcaaacc tctggccgag caccttaatc tacctgcacc gcaaagcact
    35701 ctagaactct ttgagaaaga atacttgctt cgtgtatccg ctgaaggaat acatgtacaa
    35761 ggtctccacc caatacgctc gacaatgctg accgagctgc tcaccgatgc cacgtttgct
    35821 ccgtggtttg agggagcagt ctcgtgcctg ccgtttgtag aggaagcgga tcttgaaatc
    35881 ttcttgctac attgcttttc gcagcatcgc gaacatcgta acgagctgct ttattcactg
    35941 aagttcataa aaccgatcag ctggattgga attattggcg tggtacgagc tctgatctgg
    36001 gcgggagtcg ccgagtatgt ggaagccaac cggaagctca ttgaggacgc tgtagcgcaa
    36061 ataggcatcg gtggctggtc atacttcatt gatttcgata tcgctaatgt aagcgacagt
    36121 actgccgcgt cctggtggag tgagttggat ttcatcactg acgaggtacg agaaaaaata
    36181 aaggaactac agtcacacca aacagataag aaacaggtat ttcaacacgc caccagctgg
    36241 ctgaccggtt acagtatcaa gccagttccg ccaacaactg aagctgactg gtctgcgatt
    36301 gcagaaactg ctttttgggt gggccgccta aatgttggct ggccactgcc acagtcgctc
    36361 actgagactg aacttgagtg ggcaatcgaa aaattgtctc tcgagatcct ggccaacttg
    36421 gtatcaggtc tattcgaaaa cgaagagttc cacaactggt ttttaaccaa ccggaaaagg
    36481 ttaattagcc gctttcggca agacaccgac acggtaatgc tcgaagatga tggtcataga
    36541 ctaaccgcac ggttcatctt tgccttacct gatttggttg atgcaccagc cgacgataca
    36601 caaaacatct cggcgaaaac gaagaactcg tttcacgatg cagcgattca aagactcgga
    36661 ctattgcgaa gtctgtttcc tgatcgagaa gagttttcga cccaagggta tgggcacatg
    36721 ttgtgggaga attttttgga atttgacgag acgattaaga ccggcgtcga tcgtaagcac
    36781 cttccgttag tatggttgac gtcggttaat gccacgttcc gcggtttagg aaatttgcaa
    36841 ttcaggcccg ccacctggct tgagtactcg ggacgcatct tagacatccg acgatcggtg
    36901 ctttcttcac tgaagcaact tgagcgcgga attgaaaatc acttccgacg cacccgagtt
    36961 accaagatgc tgggaacaga cgtcaacggc gaacagtggg accgttgcaa gatgatgctg
    37021 actcatacgc ctttgctgcc gagagtagcc gtcgacgaat ggggcttcct agaagagtca
    37081 acctcagaca taaacaacag gggcatcgca gagcgattaa cgcagagaag cagcatagcg
    37141 gcagggaggt acaaggcatt tctaaaggca ttcaatgaac atacccgtac tctatctaac
    37201 ttctttggcc aagccaccga ttgcatgata acaaacgctc ttctgggcag agcaagcgac
    37261 agaaccaaag tgttggaagc tgcttcctct gtcggaatca aaccaaactc atgcaagctt
    37321 tccacactga attttgccga cagccttaaa aacctgcgcg cattccaacg tgaagcgcga
    37381 acaatattaa accccttctt tgacgaccag gagcttaatc ggctggatga agaagaacag
    37441 cggagcctcc accgcatctg gtgcgcctgg tacttctttg cgcatcagcc acaaaaagta
    37501 atgcagaacg cggttagtgt cagcgaacgt gaatgcttac agatcttaag aacgatccgg
    37561 ttggatctgc gaggcgggct tcgtcggatt tcttcggacc tcgtccgctt caatatcttg
    37621 tctgaggata ctctgtggga tgatcggccg gctctttggt taagtgttga tgctgactct
    37681 cctttagccg ccctaagctc cttgccaact gttctggacc aagtgagaca atccatacaa
    37741 aaggtagcga aaagtgatct cagacgttac gttcttgatt tgaactggcc acatgttgtg
    37801 ctcgtgcctt tagtgaaagg taagtgtcca aacacggttg catggcgaat agccttgccg
    37861 gtaatcacgg agagtaaaaa tgaaagctta agttggtgga attacgctca gtctccgatt
    37921 ccaactgagg ctgtaaaaca cctgaagata gattcctgga atcttcctca actgagcgta
    37981 gcggccaaac tgatgcaata cacaacaatt ctgttctata tagcagttca cattcgcgac
    38041 ttctgtcgcc tacccgacct tgatgaggaa ggactgacgc agttgcagtc ttacgttagt
    38101 cggctaagca gacacgcaag tgaggcactg caatcggtat tggacacgga ggtagagatg
    38161 ctcgcggcat tcaacaacct ctccgaagga gatcaaaaac tccggccgta tctcttcgag
    38221 tcagctgtgg cgataacgaa tttacacaag gcaattttac catcggagga tttcagtaac
    38281 cgtctagaga tggacctaga ggggctaatc gactgggcca cccgattgga acaggctcct
    38341 gcccatgctc ttgctgcatc ctcagcctgg atggcagatg tgatcgacca ggacctaagt
    38401 gatcaggcac cttctggtta gtcctctcac tgtcgcttgc aagccgctat aaacaaaacg
    38461 actactcaga ctcggattca cccagggtcc caaagtttac ttcacaatct ggaggtagaa
    38521 cttgacagac atagagttgg ctattgctac gcaaacagag ttcgaagaat tcctcaggca
    38581 cacacttgat gccactggta attcatttgc cgaactcatc atgagcatca aggaacgttt
    38641 gacaaggagt cttgtcgacg atttgcacta cttgagaagg gagcgaaata gtctggcaca
    38701 tcgacgtaaa cgtgagttgg attctcgtca aaaatttgaa gaggtctcct cgcgagcgag
    38761 ggagtcattg ttgagagtag cggtccctac cctctcagac atcggttatt cgatagtgaa
    38821 caagaagagt ggaaagtctc tggacgttcc ctgggaaatg aacaactaca tagttcatca
    38881 gtggagctat cattgcggca ctaatcaaca ttggtgcttg cgaagaaccg ctgagaagca
    38941 cattgccatc gtgtcccgtt atactggcag atgcctcgac gtagagcgca gtagcgattc
    39001 ggacaacgct gctgtgactc agtggaaata ccatggtggc ttgaatcagc aatggattct
    39061 gacccgcctt gaagatcact cttatcaaat acgagctgca cacagtggca aagtgctaga
    39121 tattgtttgg gccagtgatg atgatggcgc gactacaaca caatatcctt ggcacggtgg
    39181 cgacaatcaa agatggtgga taggcgccgc gctttaaagt tattccgatt tttaatgtta
    39241 ggccaactaa cttgttccgc gcagctatag ccttagaaaa atggttcttg gtagtgagaa
    39301 gtttcaaagc tcggggcaat aatgatcaat taagactcgt gttagttgct cgccacagct
    39361 ggtgattctg cgtatgctct cgtccgacat agctgcaaag ttagttggat atccaaaacc
    39421 tccgcgatgc actaaccctg ccttgcaacg tcacggagct gtcgatgtgt cgaatgctcc
    39481 ccaaaccgtc aagataacgg agctaccgct gccgtaatac ccaagcttcg cccgtttggg
    39541 cctctcacat aatctaaatc ccagttgatt gccggtaatc gcaaggccat aacacatcgg
    39601 caacttggtc ttggaacgcg acgtttcacc ggcgaagttc caaatgtctt cgcttcgaca
    39661 atgtgcgaaa accgcagacg aaaaataggg cggaaaacag ttagcgatct gaaggtggtc
    39721 gctatgtcca catttggtta tcgcaaaaga gtgttcttgg cttcaatttc gacggggcac
    39781 acgtcctaca ttcttactga ggtcgaatca agccgaggcg gggagtataa gtggggccat
    39841 tgtatgttaa cgatggctga ctgtcggcgt cgtatccagc tcgaattttt tctcggtact
    39901 gttcgcgccc ggcgtgagag cttgagaaag attgatctac tgatgaagca gttagaacaa
    39961 tttcggactg cgctgcgaac cgaggccggc ctgatcgaac aatacgaagc aaagcaaaag
    40021 gccaagcctc gaaagtcaaa caaagcctca aagaggaaag cagttccaaa tggacgaaca
    40081 aacaagagaa gccatgcgga ataatgttat cgcgagcact cacgctgaaa tgccttacgc
    40141 cacagtaaaa ctaactccta gcgaaattcg gccaagagac cgggtcttcc tgcaacagtg
    40201 ggccgaaaag cttggcgtgt cgattgacgt cttattgaag ggcattctgc tagcagccat
    40261 cgaagaccag ctttacgccg agaaaattcc tgagatctga cattcagata gcctcacgcg
    40321 gtatcgctga gatgacgcgt aaggctcggg catcgggcgc tgatggcctc accacgttgc
    40381 tcattgcata gtgacctcac cacatatgcg caccgctata gcgggcgcta agagtgggtg
    40441 gtgtgtttag cgccctgcag ctttcaaatg atctcaacga aggtcggaag tctaacggtt
    40501 gactttaggt taggttgagc cgttaccgtg gcccgcagcg gcgcttgtcg gttgctggta
    40561 gccttcaacg tctcccttct gcgtccattt atgattcgcg atggacggcg cgaattgatc
    40621 tcgtcacaat aaagttttcc ctgagacgaa tagccaggcg agtgcgatag cactcggctg
    40681 gcttttttgc ccccgccgca actgcgccta tggcgcagtg cggcgaaaat ttttagcgtt
    40741 aaaaatccga cgaaagatcc gacaaattct tcagtgaact cccacccgaa gttcacttcc
    40801 cttcagtacg tttgagcatt gacaaggcga gtcgaataaa ctacaagccg tatgctggcc
    40861 cctactttcc ctaaacaaaa tttcgccagc tttcccctat gttgttatct gcttgtaagc
    40921 ccttccaatg gtactttgtc gtatcgtgtc ccgaatgcaa aacgaggcaa gccctcttct
    40981 acgatccgtc gcagggtcaa gcgagaattc gccaaacata tcagcatcgg tgcgataaat
    41041 gtgggagtga aaagttctat gaaccagagg atatagaacg ttaccagcaa atcgttgaac
    41101 gtcgacgaaa accgcgcaac taacgaggag tttcgccagc tagagggcga ccaagttcac
    41161 ttcaaaacac tacttgaata cgcacgatct cctcataccg agggcgctgc gtttgttagt
    41221 tgtgtctctc ctggattttc cttcagctat cttgaaatcc atccattaat aaacgcagcc
    41281 cgaatcccaa tcgaaggcta gtgtctcacc aaccgaaaaa ccaagtcctc ccagggagcc
    41341 cttggccctt cggtttatac cgcaattcct aactcgtcgc acatcgttta gtttctcgga
    41401 cgtcgcagaa ggagcatctg cgactgtcct cgaaacaaaa gcgttttcgg caaaaacaac
    41461 ctcaaatcgg acggtcatct gataacagaa gattctgttc tcccaactac agcgcctggc
    41521 ggcgctcccg acagaatcgc tgttatcaaa cgcccgctta cttttatttg ttctccccct
    41581 tgcgcccgct tcgctcgcgc gctcggcgca cctcgcaccg ctcactacgc tacgcccccg
    41641 ctgggggctg gcgctttggt tggtgtcgtc ttggtgtcct cttaggctcc agtagaaaag
    41701 tttccagata gttgaaccga gtggcccatc cacgtgtgta accaagccgt gtgagaatcg
    41761 cgccgcggcg cttggatctg tccgcccgaa ttgcggctgc agtgttacgt aggtcacgcg
    41821 ggttaaccat tccattcaat acacgctgtg aagctcgttg
//
LOCUS       DASSFP010000351         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1291155, whole genome shotgun sequence.
ACCESSION   DASSFP010000351 DASSFP010000000
VERSION     DASSFP010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291155"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..1997
                     /locus_tag="VFH91_07930"
     CDS             378..1997
                     /locus_tag="VFH91_07930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6862962.1"
                     /translation="MDVPQFIAGRFRIEREIGTGGMGTVYLATHLGLDRPVAVKIIKR
                     EFAGDSDVTDRFLREARTMAKLRHPHAAMIFDAGHLPDGRQFIVMEFVEGVTLSQALA
                     AEGRFSLSKAVHIAMEICDVLEEAHRLGIVHRDLKPSNIILGQRGVCVLDFGVAKVLA
                     SSTEATATHASTGSGQIVGTPRYMSPEQCLGQRVGARSDLYSLGVLLYEMIAGRPPFT
                     DPLQSALLVKQATAPAPPLPKLRPDLPRSLSLAVHTLLAKRPEDRPRTAATAKALLER
                     SLVQPDRTLPDIEPLASMVAATSPGRNILFRVGAPLVLVTTFGALLAWGYTGESAPEP
                     QPQKISALPTLSQSIMAPASFRGQPSLVTPTSEEPREKARPETDLTREMARKIAARFA
                     SGSLGPVQIMDTDSGQSIVAINNQRKEGKTNFFILEKRNNKYRVTAQGPLDMDGFRRA
                     SWSSELTDADEDGYQEVLFAGKDSKESRTKRRLVLFVPNDKRTYSMFMTGETTQRGTP
                     KISWLSNATGTDAAAYRTALRQKARSLIRSR"
     gene            complement(2011..2208)
                     /locus_tag="VFH91_07935"
     CDS             complement(2011..2208)
                     /locus_tag="VFH91_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862963.1"
                     /translation="MIRGKRKQPRPIDNSVSDSTGQYDLRFVLWRQFCSQNSIPVDTL
                     PSQLNNEQREKWDELKSKRLK"
     gene            complement(2365..3168)
                     /locus_tag="VFH91_07940"
     CDS             complement(2365..3168)
                     /locus_tag="VFH91_07940"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET6862964.1"
                     /translation="MVHDDFKEMIPTYVLSALDAVENRALGEHLSQCAECQRELENWQ
                     ATAAMLSLDAQPLEPSPEVRDRIMKAVREESSQSGPESKSNVIQFKPVSNASDRSYSS
                     VWAIAAGVLFLITAGTLFLFWQESRTNNRKIAELAAQLKDVQVELDQSKKIVQLMMTP
                     GARMDELAGTTEAPGANAKLAYDKTGHAMLMAYGLPPAPTGKAYQLWFIVGNQKMPGK
                     SFSVDSSGTGTLEDQIPESARQSAVFAITLEPAPVGVVPTGPIYLRSTL"
     gene            complement(3175..3753)
                     /locus_tag="VFH91_07945"
     CDS             complement(3175..3753)
                     /locus_tag="VFH91_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6862965.1"
                     /translation="MQPEEAQIDDVALLKAIAGKDDKALSQLYDRYRVVLFGLLVRIL
                     SNREEAEDVLQEVFLQVWRRASDYDANRGRPFTWLVTLARSRAIDRLRSVASRERVVA
                     ASARDPVEETSDAIADAVRSEQRGIVTSALSQLPEEQQKPLVLAYFDGLTQSEIADRL
                     GAPLGTVKTRMRTGMLKLREALGGKSQSFGFK"
     gene            complement(3895..>4879)
                     /locus_tag="VFH91_07950"
     CDS             complement(3895..>4879)
                     /locus_tag="VFH91_07950"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET6862966.1"
                     /translation="VPGPDFVDRVWSISDRSPAVLRSLQTLFTCGRLSGTGYLSILPV
                     DQGIEHSAGASFAPNPQYFDPENIVKLAIEGGCNAVASTFGVLGTVARKYAHRIPFIV
                     KINHNELLTVPNKFDQVLFGTVDEAHRLGAVAIGATIYFGSEESTRQITEVSKAFSHA
                     HELGMATILWCYLRNPKFKTPEGDMHTAADLTSQANHLGVTIQADIIKQKLPERNGGY
                     KAVEKELGGSYGKTSKLVYEKLTSDNPIDLVRYQVANCYMGRCGLINSGGESKGASDL
                     AEAVRTAVINKRGGGMGLISGRKAFQRPMKEGIQLLNAIQDVYLADDITVA"
BASE COUNT         1180 a         1311 c         1199 g         1189 t
ORIGIN      
        1 gataagtgag gagacttcgg ttgccactgc cgttggtcat tggtcatttt ccattatttt
       61 tccatttctc atttcccatt tttcatttcc cattttttct gacgaaacgt ttcttgcctg
      121 aacgagactt atgcaaagtc aggcactgcc actgcgcaaa tgaaaaatgg gaaatgaaaa
      181 atgaaaaata gatgagggtt ccaaccccaa acctgctatt cactagtcgg cggattggtg
      241 ctaaactacg cgagccgagc cctttaaagg gtttctctcc cccagaaaaa attcaaaact
      301 gaagttgact gacgagctgt tgctctttta gctcaacggc tcttccaatc gaccttttaa
      361 cccgaaggaa ggtcaccatg gatgtccccc aattcatcgc tggcaggttt cggatcgaac
      421 gcgagatcgg gaccggtgga atgggtacgg tctatctcgc cactcacctc ggtctcgacc
      481 ggcccgtcgc agtaaaaatt atcaagcgag agttcgccgg tgacagtgat gtcactgatc
      541 gcttcctgcg cgaagcgcgc accatggcaa agctgcgtca tccgcacgca gcaatgatct
      601 ttgacgctgg tcacttacct gatggccggc aattcatcgt gatggagttt gtcgaaggcg
      661 tgaccctgtc gcaggctctt gctgccgaag gtcgattttc actaagcaaa gccgtgcata
      721 ttgcgatgga aatctgcgac gtacttgaag aagcgcatcg cctcggaatc gttcatcgtg
      781 acctcaagcc gtcgaatatc attctgggtc agcgcggagt ctgcgtactc gacttcggag
      841 tcgcaaaggt actcgcctcg tcgaccgaag ctaccgcaac acatgccagc accggctcag
      901 gacagatagt tggcactcct cgttacatgt cccctgaaca atgtctcgga cagcgcgtcg
      961 gagcacgaag cgacttgtac agtttgggcg tgttgctcta tgaaatgatc gctggacgac
     1021 caccgtttac cgatcccttg caatccgcgt tactggttaa gcaagcgaca gcgcctgcgc
     1081 cacctctgcc caaattacgt cctgatctgc cccgatcatt gtcattggcc gttcatacgc
     1141 ttcttgcaaa acgcccggaa gatcgaccgc gaacagcagc gactgctaaa gctttgttgg
     1201 aacgtagtct cgttcagccg gatcgcacct tgccggacat cgaaccacta gcttctatgg
     1261 tggcagctac gagtcccggt cgaaacattt tgtttcgagt cggagcgcca ttggtccttg
     1321 ttacgacgtt tggagcattg ctggcctggg gctacacggg tgagagcgct cctgagccac
     1381 aaccgcaaaa gatctctgcg cttccaacac tttcacaatc gatcatggcc cctgcttcct
     1441 tcagaggcca accgtctctc gtcactccga cttctgagga accaagagaa aaggcccgcc
     1501 ccgaaactga cctcacgcgt gagatggcac gaaagatagc ggcgagattt gcaagtggct
     1561 cgctcggtcc agtgcagatc atggacacgg attccggcca gtcgatagtg gcgataaata
     1621 atcaacggaa agagggtaag accaactttt tcattctcga aaagaggaac aacaaatatc
     1681 gagttactgc tcagggacca ctcgacatgg atggctttcg acgtgcgagc tggagttcag
     1741 agctcaccga cgcggacgag gatggatatc aggaagtact ctttgccggt aaggattcga
     1801 aagaaagtag gacaaaacgg cgattagttt tgtttgtacc aaacgataag cgcacttact
     1861 cgatgttcat gaccggggaa acaacccaga gaggtactcc taaaatttcg tggttatcga
     1921 acgcgacggg aacagatgcg gcagcttatc gaactgctct cagacagaaa gcacgctcac
     1981 tcatcagaag tcgctgattt ggccaaaaac ctacttcagc cttttagact tcaactcgtc
     2041 ccacttctct ctctgctcgt tattcagctg actgggtaac gtatcaactg gaatactgtt
     2101 ttgcgaacag aactgtcgcc ataaaacaaa acgaagatcg tactgcccgg tggaatctga
     2161 tacggaatta tcgattggtc gtggctgctt ccgctttcct ctaatcatca acttcactct
     2221 tcacgcaaat taggtggggg aagtttagca ggttttgccg gagaatttca tcgtgcgacg
     2281 gattcgttac aaaagaatcc ttcaaatgga ccgttctctg gtcgttattg gaaaaaactg
     2341 taggaggtga aaagctggtc acttttagag agtactacgc agatagatgg ggcccgtcgg
     2401 cactacacct accggcgccg gttcaagcgt tatagcaaac accgccgatt gccgagcaga
     2461 ttcgggaatc tgatcctcta aagtccccgt cccggaacta tcgaccgaga atgacttacc
     2521 aggcatcttt tgatttccaa ctatgaacca caattgatat gccttgccgg tcggggctgg
     2581 aggtaggcca tacgccatca acatcgcatg tccggtctta tcgtaggcca acttcgcatt
     2641 tgctccgggt gcctcagtcg tccccgcaag ttcatccatc cgggcgcccg gtgtcatcat
     2701 tagttggacg attttcttcg actggtccaa ctcaacctga acatctttaa gttgcgccgc
     2761 gagttctgca atctttctat tgttcgtccg gctctcttgc caaaacaaaa acaacgttcc
     2821 ggccgtgatt aaaaaaagta ctccggcggc gatggcccaa accgagctat aacttcgatc
     2881 agaagcgtta ctcaccggtt tgaactgaat cacatttgac ttggattcag gaccagactg
     2941 cgatgactct tcacgtacgg ccttcattat tcgatcgcgc acttccggcg atggctcgag
     3001 tggctgcgcg tcgagcgaca gcattgctgc cgttgcttgc cagttctcga gttctcgctg
     3061 gcactctgca cactgtgaaa gatgctcgcc cagggcacga ttctccaccg cgtcaagcgc
     3121 agacagcacg tacgtcggaa tcatctcttt gaagtcgtcg tgaaccatca ttctctactt
     3181 aaatccaaaa ctctggctct ttccacccag ggcctcacgc agttttagca tccctgtccg
     3241 catcctcgtt ttgacggtac ccagaggcgc acccaaccga tcagcaatct ctgattgggt
     3301 cagaccgtca aagtaggcca gtactaaagg tttttgttgc tcttcgggga gttgtgagag
     3361 cgcggaagtt acgattcctc tctgctccga tctaaccgca tcagcgatag catcggaagt
     3421 ttcctcgaca ggatctcgag cactggcggc aaccacacgt tcgcgagacg cgacacttct
     3481 gagtcgatcg atagcgcggc tccgggcaag cgttaccagc cacgtaaatg gccgccctct
     3541 gtttgcatcg taatcgcttg cccgacgcca gacttgaaga aagacttcct gtaagacatc
     3601 ttcagcctct tcgcggttac tcagaatcct gactagcaaa ccgaacaata caacacgata
     3661 tcgatcgtat agctgagaca gagccttatc atccttgccg gcgattgcct ttagcagggc
     3721 tacatcatcg atttgtgctt cttccggttg caaggtttta ggccgcgcct ctcagataaa
     3781 tacgagcgag cgcggaaaac agattgaccc gcaaagcaga aaaatcaggg cgctttaacg
     3841 acgccctgat tttcaagata actcagtcct tattaggact tcacgtagtg ggggctatgc
     3901 gacggttatg tcgtctgcaa ggtaaacatc ctgaatggcg ttcaggagct gaatcccttc
     3961 tttcatcgga cgttgaaacg ccttgcggcc ggaaattagg cccatgcctc cgccgcgctt
     4021 gttgatcact gcagttcgga ctgcttccgc cagatcactg gcccctttgg attccccacc
     4081 cgaattgatc agaccacagc ggcccatgta acagttggcc acctgatagc ggacgagatc
     4141 aatcggatta tctgaagtca acttctcgta aacgagtttg ctggtcttac cgtaagaacc
     4201 gccaagctcc ttttcgaccg ctttgtagcc gccatttcgt tcgggaagtt tttgcttaat
     4261 gatatcggcc tgaatagtta cgccaagatg atttgcctga ctggttagat cggctgcagt
     4321 atgcatgtca ccttctggtg tcttgaattt cggattccgc agataacacc aaagaatagt
     4381 tgccatacct aactcatgtg cgtgagagaa tgccttgcta acttccgtga tctgtcgagt
     4441 tgattcctcg gatccaaaat atattgttgc gccgatggca accgcgccta gccgatgtgc
     4501 ttcatctacg gtcccaaaca gcacctgatc gaatttgttg ggaacagtta acaactcgtt
     4561 gtggttgatt tttacgatga agggaatcct gtgtgcatat ttgcgagcca ctgttcccag
     4621 caccccgaag gttgaggcga cggcgttaca cccgccttcg atggcaagtt tgacgatatt
     4681 ctccggatcg aagtactgag ggttaggagc aaatgaagca ccggcagaat gctcgatgcc
     4741 ctgatcgacg ggcaggatcg ataggtagcc tgtgcctgaa agcctgccac aagtgaaaag
     4801 tgtctgcagc gatcttaaga cagcaggaga acgatcggag atagaccaga cgcgatcgac
     4861 gaaatcagga cccggaacc
//
LOCUS       DASSFP010000352         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_695397, whole genome shotgun sequence.
ACCESSION   DASSFP010000352 DASSFP010000000
VERSION     DASSFP010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695397"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VFH91_07955"
     CDS             complement(<1..978)
                     /locus_tag="VFH91_07955"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6862967.1"
                     /translation="MSESNNTKRIAIFLIACAFALALTLLFWSVSASIQAFLTEKTIR
                     NPSPETVVQVLKLLFVGILGYLLVKALNFLFFDLLFRFRQGFDAPTLVRNVFSIIAYT
                     IFFFIALKALFPDLNLGALFTTSAIFGVIFGLALQDTLGNFFAGISLQADRPFQVGDV
                     ITVGALRHTGVVEEISWRAIKIRTFQNHVVLIANSTAAKEPIEVCPRDNLNARLVFFN
                     TLYTDSPAKTIHVVREAVREADNVSEKITPIVRIRNLGDNGVDYEVKYWLIDYAKYND
                     TDALVRQRIWYAFRRAGLNFAYPTRSLIVERKGGQGLTERDGGAIVERLS"
     gene            complement(1074..2387)
                     /locus_tag="VFH91_07960"
     CDS             complement(1074..2387)
                     /locus_tag="VFH91_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6862968.1"
                     /translation="MADSRAAVRAAGRRLMRRGARGVRSKIGRRLLVYLAILGPGMIT
                     ANAGNDAGGIATFASVGAEFGYSLLWILIPITISLGIVQEMCARMGAVTGKGLADLIR
                     ERFGVRWTALVMLALLIANAGVTVSEFVGIAAATELFGVPRFISVPLAAIAIWWLVVK
                     GSYQRVERVFLLMSLVFLSYIISAFLAHPNWGEVALGVVRPTFRFEQVFIFTFVAVIG
                     TTISPYMQVYVQSSVVEKGVRAEDYLKTKLDVWVGTIFAVVIVFFIVVSTAATLHKAG
                     IQVTSAADAARALSPLAGRYAETLFGVGLFGASMLAAGVLPLATAYSISEALGFEKGV
                     SRSFREAPIFLGTFTFLVAVGAAIAVIPNLPLIRVLLVTQVINGLLLPVVLFAVMRLV
                     NNRELMGEKVNGPIYNLAAWLTVLVVTMLSLLYILITLVPGLIPD"
     gene            complement(2415..3647)
                     /locus_tag="VFH91_07965"
     CDS             complement(2415..3647)
                     /locus_tag="VFH91_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6862969.1"
                     /translation="MSQVLGRPIRDLEGERVATVKDVIVRLGADDHPPVTGLVARYRR
                     RDFFLSRWRITELNQHGARLNSDILDLRPFVRRESEVLLARDVLDKQLIDVDGKRVVR
                     VNDVQIIEAAGDWRVTGADVSLQGLWRRLAPSGFVGTKAPVEVLDWADVGYLATDAAT
                     VQLKSSSDKLARLHPVEIARLAEALSYHHGSEVVEALDDETAAETLEEMAAEKQARIL
                     GDMDQERAADILEWMSPDEAADVLGDLPEEKAEELLGLMEQDEQADVAELLPYADDTA
                     GGLMTTEFVTLPREFTVGEALSRLREMAETPNMIYYLYVVETEGSWKLLGVIALRSLI
                     LADPAMPLEDVMRTEFQRAEPDESAKEVAQRIAEYNLLALPVVDEAGDILGIITVDDA
                     MEILLPKDWRQRLPRLFS"
BASE COUNT          983 a          997 c          931 g          822 t
ORIGIN      
        1 agaaagtcgc tcaacaatag ctccaccgtc gcgttccgtc agaccttgtc cacctttgcg
       61 ctcgacgata agacttcgag ttggataggc gaagttgagc ccggcacgac gaaacgcata
      121 ccagattctc tgtcgcacta aggcatccgt gtcgttgtat tttgcgtaat ctataagcca
      181 atacttcact tcatagtcga cgccattatc acccaaattc cggatacgaa cgatcggagt
      241 gattttctct gaaacattat cggcctcccg aacggcctct ctaactacgt gaatagtctt
      301 cgcgggtgag tcggtataca aggtattgaa gaagaccaac cgtgcattga gattgtcacg
      361 ggggcaaacc tcgattggct ctttggcagc ggtgctattc gcaatcaaga caacgtggtt
      421 ttgaaatgtg cgaatcttga tggctcgcca actgatctcc tctacaactc ccgtgtggcg
      481 cagggcaccc acggttatta catctccgac ttgaaatggc ctgtcggctt gaagcgaaat
      541 accagcaaag aaatttccca gcgtgtcttg cagtgcaaga ccgaaaatta caccgaagat
      601 cgccgaagtg gtgaacaaag cgccaaggtt cagatccggg aatagagcct tgagggcgat
      661 aaagaaaaag attgtgtagg cgattatgga gaaaacattg cgaacaaggg tgggagcgtc
      721 aaagccttgc cgaaagcgga agagcagatc aaagaagagg aaatttaagg ccttaacaag
      781 tagataaccg agaatgccga cgaataataa tttcaacacc tgcaccacgg tctcgggtga
      841 aggatttctg atcgtctttt cagttaaaaa cgcctgtatc gatgcgctca cggaccaaaa
      901 aagaagtgtc agtgccaacg caaaggcaca ggcgatcaga aagatggcaa ttcttttggt
      961 gttgttggat tcgctcactt ggtctcggaa cgacgaataa gattttacga ctctgtccgg
     1021 cgggctgatc ttttatcacc aattcaggct aattgacgag aagattctgc gggtcagtct
     1081 ggaatcaggc cgggaaccag cgtgataaga atatagagaa gcgagagcat tgttacgacc
     1141 agaacggtga gccacgcagc gagattatag ataggaccgt tgaccttttc gcccatcaat
     1201 tcgcgattgt tgaccagtct catcaccgca aatagcacca ccggtaacaa gagcccattg
     1261 ataacctgcg ttactaaaag gactcgaatc aacggcaggt tgggaattac cgcgattgct
     1321 gctccgaccg ccacgagaaa agtgaaggta ccgagaaaaa ttggagcctc ccggaaactg
     1381 cgggaaaccc ccttttcaaa tccaagcgcc tcgctaattg aataagccgt tgcgagtggg
     1441 agtacgccgg cagcaagcat cgatgcacca aagagtccca caccaaaaag tgtctcggcg
     1501 taccgtcccg ccaatggact caaagcacgt gctgcatccg ctgcgctcgt tacctgaatt
     1561 cccgctttat gaagagtcgc agcagttgat accacgatga aaaatacaat caccaccgcg
     1621 aagatcgttc cgacccaaac atcaagtttc gtcttgagat agtcttccgc tctaacgcct
     1681 ttctcaacga cagatgattg cacatagacc tgcatgtacg gtgagatggt cgttccgatt
     1741 accgctacaa acgtaaaaat aaacacttgc tcaaatctga aggtaggtcg taccacgccg
     1801 agagcaacct ctccccagtt tggatgagct agaaaggcag agatgatgta gctaagaaaa
     1861 acaagtgaca tcagtagaaa cactcgttcg actcgctgat atgagccttt gactaccaac
     1921 caccagattg ctatcgctgc aagtgggacg gagatgaaac gtggtactcc aaataattcg
     1981 gtcgccgcgg caatgccgac gaattccgaa accgtaactc cagcgttggc gatcaacagc
     2041 gcaagcatga ccagcgccgt ccatctgaca ccgaaacgtt cgcggatgag atcagccaga
     2101 ccttttcccg tgacggcgcc cattcgagcg cacatttcct gaacaatacc caggctgatt
     2161 gtgattggta tcaggatcca aaggagggaa tagccaaact cagcgccgac tgaagcaaag
     2221 gtcgcgatac cgcctgcatc attgccggcg ttggctgtaa tcattcctgg gcccagaata
     2281 gcgagataaa cgagcagacg gcggccgatt ttgctgcgca caccacgagc gcctcgccgc
     2341 attaaacgac gacccgcagc tcgaactgcc gcacgtgagt ctgccacttg gaggttctcc
     2401 gtccgtcatt tcactcaact aaataatctt ggcaatcgct gtcgccagtc tttaggcaaa
     2461 agaatttcca tcgcgtcatc gactgtgatg atacccagaa tatcaccagc ttcatcaact
     2521 accggaagag ccagcaggtt gtactcggca atacgttggg ccacttcttt ggcagactcg
     2581 tctggctctg ctctctgaaa ctctgttctc atcacatctt ccagaggcat tgccggatca
     2641 gccaggatga ggcttcgtaa tgcaatcacg cccagtagtt tccacgaacc ctcggtttca
     2701 actacgtaga ggtaatagat catgtttggc gtttccgcca tctctcgcaa acgcgaaaga
     2761 gcttcaccca cagtaaactc tcgcggcaag gtgacgaatt cggtcgtcat caagccgccg
     2821 gctgtgtcgt cagcgtaagg caataactca gcgacatccg cttgttcgtc ctgctccatt
     2881 aagccgagga gttcttcggc tttttcttca ggcaaatcgc ccagcacgtc cgccgcctca
     2941 tcaggtgaca tccattcgag aatgtcagct gctcgctcct ggtccatgtc accgagaatt
     3001 ctggcctgct tttcggcagc catctcttca agcgtttctg ctgcagtttc atcgtcgagc
     3061 gcttcgacaa cttccgagcc gtgatggtac gagagggctt ctgcgagtcg agcaatttca
     3121 accgggtgga gtcgggccaa tttgtcgctg gatgatttga gctgaacagt tgcggcgtcg
     3181 gtcgcgagat agcccacgtc agcccagtca agcacctcaa ccggcgcctt agtgccaaca
     3241 aatccagatg gggcaagacg acgccagagt ccctggaggc ttacgtcagc tccggtcacg
     3301 cgccagtcgc cagccgcttc gataatctgt acatcgttga cacgcactac acgtttgccg
     3361 tcgacatcga tcagttgttt gtccaacacg tcgcgggcaa gaaggacttc gctctcccgg
     3421 cggacaaatg gacgcaggtc cagaatgtcg gagttcaatc gagcgccgtg ttgattcaac
     3481 tctgtaatgc gccagcgaga taaaaagaag tcgcgtctgc ggtaacgcgc cacgaggcca
     3541 gtaacgggtg gatggtcatc cgcaccaagt cggacgatta cgtctttaac agtagcgaca
     3601 cgttcgcctt caagatcgcg gattgggcga cctaatacct gcgacaaata gagcatcgac
     3661 taaaccaccg tgatttaatt gaactccagc atcatagcat gacgtgggtc gaggtctgag
     3721 atggcccaaa ggt
//
LOCUS       DASSFP010000353         5516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1875523, whole genome shotgun sequence.
ACCESSION   DASSFP010000353 DASSFP010000000
VERSION     DASSFP010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875523"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFH91_07970"
     CDS             complement(<1..184)
                     /locus_tag="VFH91_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862970.1"
                     /translation="MRDKLIRALRATLILIVVCFLSLSTMGQQTVDKMVATVNAGART
                     EMITYSDLVWQLALQPN"
     gene            complement(201..3599)
                     /locus_tag="VFH91_07975"
     CDS             complement(201..3599)
                     /locus_tag="VFH91_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET6862971.1"
                     /translation="MQDASNVGTQFPSARLKSRCFRTAISFQLLLILAIAVHAAGTRD
                     ARFDDYEGRTVSDIEVVFEGSPQDPAAQSEFLTILRISPNSEYSAERIHDSLLSLFES
                     GRVANARVEVIESSPAKTGPIRLRFVIQRQVQIGAVRLELGPVTGSPISTDELRARLN
                     FVQPGTRLTKQIIARNADEIQVYLRDRGYFNATVDSVEDVDSTGVRATVTYRITPGEQ
                     ARVGAFQINITGFNPATVKDSLALQPSAPFTREALAADVTKIRQRMIADGFLSPVLDD
                     ARVERDSEKNTITIRLSGNKGPQVNVTVKNYEVSEKTQRELFPVKREGSIDFSAIVEG
                     ERRLRNKLQEEGYFFAEVSETCTVTDPPAELGPNGTDETCQNLNPALLNGRKVDIAYS
                     VDAGRRFRLSDIRITGTNKLSFGDVEADLKSQKANAIGLIPFLGYGRGYTSLALLEQD
                     KRTIEAFMRDIGYRKARAEILQGVSLNGEDLIITFQITEGPLTRIAGVEIRGNKMYTE
                     QQIRDELHTVVGAPFSRSQARADGDRITTMYARQGFVSARMDFSIVELPKKGDDEQVR
                     VVYTIADEGDKVFVNQIIINGVTGTANTQRSKRDAILRAIPISSGDVLRADRISDAER
                     ELYLTDAYRQVIIRTEPAGENPSGYKRRDLIIDVEEKKPRVMDYGGGYSTDAGVLGLF
                     DISHVNLMNKLRTGAMRIRASRLQQLVRFEYIDPRFARYSKTQFAPLAASVEYQRDST
                     LTRFFRSAIDRGTMGIVQRLDENGNPIDIFGNQVSEPTINRFTAALETQRILNQKTRT
                     IIFGRYSYEDVRLFNLESLVVKPILEPDRKIRLSKFAVSLVRDTRERCERGLLNLRRA
                     TDDDESAGGPGEVCRYNQIDATRGDFLSVDYAVSLRQLGGNISYNRFQATYSRYHKLQ
                     SFRNTVLAGNFTLGLANIFNPRDRDGTGTIDEIDLTLPISERFFSGGSTTLRGFNFEE
                     AGPRQVVIPLGPFRDSNKNIVFLNPFTVPVGGNALAVVNLEARIPLTKTFQAVPFYDG
                     GNVYRRIGDLFGKQDTTPVPPGDLLAAITAANLRAKWSHTVGFGFRVQTPFGGALAID
                     YGVLLNPPEFLIPQRSPNGIDFNGTPAIFRLGQGHLHFRITQTF"
     gene            complement(3652..>5516)
                     /locus_tag="VFH91_07980"
     CDS             complement(3652..>5516)
                     /locus_tag="VFH91_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET6862972.1"
                     /translation="GNYAEQDFSGYQITFDGSGQNVTINGRDAGLVALKGRTENRQLN
                     ITFTTGVFGSDQVVSANIDLSTKRLATSLQTKLSNVDLTRVFALVLPAGTVRVSGKAN
                     GDVTASGFLLDEDGYFTLDALAGTATFSELSLAVEDIQLSAAAPLVVRFSPSEVTLED
                     ARFTGPGTNITLAGTVATASGGKQNLNVTGSLNMRVLNGLSPDFFSSGNAEVAVRVNG
                     TYEAPRVTGTASVNNSSVSVLLGNERWTISNLNAALRFTANQAQIDSLSGTIGGGRVT
                     ATGGALLNGFTLAGFRVNLHGDDVTVPFPQNFRSTLDADIEIKGSSREQLISGIVNLR
                     RAEFSQNIELADLINSRTESIEEGGELEITRTALFAALRVEGRNALVVRNNLADLVGS
                     VSLQLDGPVNDPTISGRITATSGTLNFRNDRYDITRALVDLPPSRNADPILNIQGESQ
                     IRGYRVIVSLTGPLSQPQAAVRAEPALPQADVVSLITTGQLSADDTSTSILSQSSVGA
                     ATSLLTDALINAPAQRATSKLFGLTRFEIDPVVGGRTGSTPSARLTLGRRISKEVTVT
                     YSTNVTSDPNQILALEYRVSDRLSFLAQYEQASTRSLTARTNIFSFEIRFRKRF"
BASE COUNT         1328 a         1448 c         1376 g         1364 t
ORIGIN      
        1 tatttggttg aagtgccagc tgccagacaa gatctgagta ggtaatcatt tcagtgcgcg
       61 cgcctgcatt gaccgttgca accatcttat cgacagtctg ttggcccatc gtcgacaggc
      121 tcagaaaaca gacgacaatt aggattaacg tagctcgcag cgcacggatt agtttgtctc
      181 gcattcacct tcctccactt ctagaaagtt tgcgtaatcc tgaaatgcag atggccctgt
      241 cccagacgga aaatggccgg tgttccgttg aaatcgatac cattgggact acgttgcggt
      301 atcagaaact ccgggggatt aagcagcact ccgtagtcaa tggcgagcgc gcctccaaag
      361 ggtgtttgaa cgcgaaaacc aaaaccgacc gtgtgcgacc atttagcacg taagttggca
      421 gcagtgatag ccgctaataa atcgcctgga ggaacaggtg tcgtgtcttg tttcccaaaa
      481 agatccccga tgcgacggta gacattgcct ccatcataga agggcaccgc ctggaacgtt
      541 ttggtcagag gaattcgtgc ttcgaggtta acgacggcca gagcgtttcc gccgactgga
      601 acggtaaatg gattcaggaa cacaatgttc ttgttcgagt ctcgaaatgg accaagcgga
      661 atgacgactt gtcgtggacc agcttcctca aaattgaaac ctcgtaatgt ggtagagccc
      721 ccggagaaaa atctctcact aataggcaga gtcaaatcga tctcgtcgat agttccagta
      781 ccatcccgat cccgtggatt gaaaatattg gcgagaccga gcgtgaagtt ccctgccagc
      841 acggtgtttc tgaaactctg aagtttatga taccggctgt aggtagcttg aaaacgattg
      901 tatgaaatgt tgccacccag ttgtcgaagc gagacggcgt agtcaacgct caagaaatcg
      961 ccgcgcgtgg catcaatctg gttataccga caaacttcac cggggccacc agcactttcg
     1021 tcgtcgtcag tagcgcgccg aagatttagc agacctcgct cgcagcgttc gcgcgtatct
     1081 ctaacgagcg ataccgcaaa cttcgacaac cgtatcttgc ggtcaggctc aagaatcggc
     1141 ttgaccacta aactttcaag attgaataag cgcacatctt cataactgta acgaccaaag
     1201 ataatcgttc gagttttttg gttgagtatt cgctgcgtct cgagcgctgc ggtgaatcgg
     1261 tttatcgtcg gctcgcttac ttgattgcca aagatgtcga tgggattgcc attctcatcc
     1321 aatctctgaa cgatgcccat cgtccctcga tctattgccg agcggaaaaa acgggtcaat
     1381 gtcgagtcgc gctggtattc aacagaagct gcgagtggcg cgaattgtgt tttgctgtat
     1441 cgagcgaaac gcgggtcaat gtattcaaac ctaaccaact gttgcaggcg gctggccctg
     1501 atgcgcatcg ctcccgttct gagcttgttc atcaggttga catggctgat atcaaacaag
     1561 cctaaaacgc cagcatctgt cgaatagcct ccaccgtaat ccatgactcg cggcttcttc
     1621 tcttcaacgt cgataattag atcgcgccgc ttataaccag aggggttttc acctgcaggc
     1681 tcagtgcgaa tgatgacctg acgatacgca tcggtgaggt atagttctcg ttctgcgtca
     1741 gaaatgcgat ctgctcttaa gacatcgcca ctagagatcg gaatagcgcg caaaatggca
     1801 tcgcgctttg accgctgagt attggcagtg cccgtaactc cgttaattat tatctgattg
     1861 acaaaaacct tatcgccctc atcagcgatc gtatagacca ctctgacttg ctcgtcgtcg
     1921 ccttttttcg gcaactcgac gatcgagaaa tccattcgcg cactgacaaa accctgacgc
     1981 gcatacatag ttgtaatgcg atcgccatca gccctcgcct gcgagcgcga aaacggtgca
     2041 ccaacgactg tgtgaagttc gtcgcgaatc tgctgttcgg tgtacatctt attcccgcga
     2101 atctcgacac cagcgatacg cgtcagtggt ccttccgtaa tctgaaaagt gatgattagg
     2161 tcttcgccgt tcagcgagac accttgcagg atttcagcgc gcgcttttct atatccaatg
     2221 tcgcgcatga aagcttcaat cgtccgtttg tcttgttcga ggagggcgag actggtgtaa
     2281 ccgcgaccat atccgagaaa cggaatcagt cctatcgcgt tggccttttg gcttttcagg
     2341 tcagcctcaa catcgccaaa gctcagttta ttggtgccgg tgatgcgaat gtcactgagc
     2401 cgaaacctac gtccagcgtc aacactgtaa gcaatgtcaa ctttccgtcc gtttaagagg
     2461 gccggattca aattttgaca cgtctcgtcg gtaccgttcg gccctaattc ggccggagga
     2521 tcagtgacag tacatgtttc tgatacttct gcgaaaaagt agccctcttc ctgaagcttg
     2581 tttcgcaggc gccgctctcc ttcaactatc gccgaaaaat cgatgctgcc ctcacgtttt
     2641 accgggaaca attcgcgctg cgttttctcg ctgacctcat agttcttgac agttacatta
     2701 acctgcggtc ccttgttgcc actgagacgg atcgtaattg tgttcttctc tgaatcgcgc
     2761 tcaactctgg cgtcgtccaa cacgggcgaa agaaacccgt cggcgatcat cctctggcgt
     2821 attttcgtta cgtccgcagc gagcgcctca cgagtaaacg gtgccgatgg ctgcagcgcc
     2881 agactatctt tcaccgttgc aggattgaat ccagttatat tgatctgaaa tgctccaact
     2941 cttgcctgct caccgggcgt aatgcgatag gtaaccgtag ctctcactcc agtcgaatca
     3001 acatcttcaa cggaatcaac agtcgcattg aagtagccac ggtcacgcag gtatacctga
     3061 atttcatccg cgttacgcgc aatgatctgc tttgtgagcc gggtgcccgg ctgcacaaaa
     3121 ttcagacgtg ctcttagttc atcagtcgaa attggcgaac ccgtcactgg tcctagctcg
     3181 agccggactg cgcctatctg cacctgtctc tgaattacaa agcgaagccg aattggacca
     3241 gttttagccg gactgctctc aatcacttca actctggcat tggccacccg accagactca
     3301 aataacgata gtaatgagtc gtggatgcgt tcagccgaat actctgaatt tggtgagatt
     3361 ctgagaattg ttaggaattc actttgcgca gctggatcct gcggcgaccc ttcaaataca
     3421 acctcaatgt ccgaaactgt tcggccttcg tagtcatcga atcgggcatc tctggtaccg
     3481 gcggcgtgaa cagcaatggc caggatgagg agaagctgaa aagagattgc ggtcctgaaa
     3541 cacctcgact ttagacgggc acttggaaat tgggttccca cgttcgaggc gtcctgcatt
     3601 cttcttttcg cttccgtcga taaaaacaac aatcttttcg gcaccccttg ctcaaaatct
     3661 ctttcgaaag cgaatctcaa aactaaagat attagttctg gctgttaaac tgcgagtaga
     3721 cgcctgctca tactgagcga gaaacgacaa gcggtctgac acgcgatact caagtgcaag
     3781 aatttggttt ggatctgacg tgacgttggt cgagtaagtc acggtcactt ctttgctaat
     3841 tcttctacca agtgtcagtc gtgccgatgg cgttgaaccg gttcgtccac ctacaactgg
     3901 atcgatttca aaccgcgtga gaccaaacag cttgctggtt gcgcgttgcg ccggcgcatt
     3961 gatcaaagcg tcggtcaata aactcgtcgc tgctcccaca cttgattgcg aaagtatcga
     4021 ggtactggtg tcgtcggctg ataattgacc ggtcgtaatc agcgacacca catctgcctg
     4081 tggcaacgca ggttcagctc tcactgcggc ttgcggctga gataacggtc cagtgagact
     4141 cacgatgacg cggtatccgc gaatttgtga ctcgccctga atattcagga tgggatcggc
     4201 attgcggctg ggaggaagat ccacaagcgc gcgcgtaatg tcgtagcgat cattccgaaa
     4261 attcaaagtg ccactggtag ctgtaattcg tcccgatata gtcgggtcat tgactggtcc
     4321 atccagttgc aacgagactg accctacgag gtcagccagg ttgtttcgca ctacgagcgc
     4381 atttctaccc tcaactcgta aagccgcgaa tagagcagtg cgagtaatct caagttcgcc
     4441 gccctcctcg attgattctg ttctcgagtt aattaagtca gcgagttcga tattctggct
     4501 aaattcggcg cgtcgcaagt tcacgatgcc gctgatcagc tgctcgcggg acgagccctt
     4561 gatttcgata tcagcatcga gagttgaacg aaagttttgc ggaaacggaa cagttacatc
     4621 atctccgtga agattgacgc gaaacccggc gagtgtaaat ccgttgagta aagccccacc
     4681 tgtcgcagtt acgcgaccgc caccaatagt gccactcaac gagtcgatct gcgcctggtt
     4741 cgcggtaaat ctcaaagcgg cgttcagatt tgagatggtc caacgttcat tgcccaacag
     4801 cacagatact gaagagttgt tgactgacgc tgtaccagtg acccgtggcg cttcgtaagt
     4861 accgttgact cgaaccgcaa cttcagcatt ccctgaagag aagaaatctg gcgaaaggcc
     4921 gttgagtacc cgcatattca agctgccagt aacgttgaga ttttgcttac ctcctgaggc
     4981 ggtcgcgacg gtcccggcaa gtgtgatgtt cgttccgggc ccggtaaaac gagcatcttc
     5041 gagcgttact tcgcttggtg aaaaacgaac tactagtgga gctgcggcac taagttgaat
     5101 gtcttcaaca gcgagactca gctcggaaaa tgtcgctgtg cctgccaggg cgtctaacgt
     5161 gaagtaacca tcctcgtcga gcagaaagcc acttgcggta acatcgccat tcgcctttcc
     5221 tgacactctc acggtgccgg caggaagaac cagagcaaac actcgggtga ggtctacatt
     5281 ggacagctta gtctgcaacg aggtcgccaa tcgcttcgtc gacagatcga tattggcgct
     5341 gacgacctgg tctgaaccaa atacgccggt ggtaaaggtg atattgagtt gccggttctc
     5401 agtgcgacct ttgagtgcta ctagacccgc atcacgaccg ttaatagtta cgttctgtcc
     5461 actgccgtca aatgtgatct ggtatccaga gaaatcctgt tcagcatagt tgccgg
//
LOCUS       DASSFP010000354         5028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_96631, whole genome shotgun sequence.
ACCESSION   DASSFP010000354 DASSFP010000000
VERSION     DASSFP010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96631"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFH91_07985"
     CDS             <1..586
                     /locus_tag="VFH91_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6862973.1"
                     /translation="NLANQARTEIVDEREQRVNDSIASIAVLYPAPYRVAIVNAAKTR
                     KIDPRFILALIRQESVFKPLAKSPAGARGLLQLTMDAAQKYAAGAGLGPIRENQLYQP
                     ETSIVLGAQYLQTLSDMFPKLLEPVAASYNGGEDNVARWLKRGKHNDPGVFTSEIGFD
                     ETKNYVQKVMSNYRVYRQLYTAELSPRGSSKVQD"
     gene            complement(570..1442)
                     /locus_tag="VFH91_07990"
     CDS             complement(570..1442)
                     /locus_tag="VFH91_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862974.1"
                     /translation="MIVRLTFAALVFIGVTGATVLIFGGREAALATDNHVQSERTVTK
                     RPPLPQGFRLEVLVNGQPLTEYFARGRTYVEAQPNVEYELRIRNPLPQRVAVALSVDG
                     LNTIDASHTTAWNASKWVIEPYQTITISGWQMSSARARRFYFTTERDSYGAKLGQTAN
                     LGVISAVFFLEKRPAITLAPPRPIYRDDQSKESRSGEPVAPSAKSDAMRDKGGVVSQA
                     PNDEFAATGIGRSVDNDVRWINMELESRPATELNLRYEYYSALRRLGVVPRPVSEPDP
                     LRRREDSRGFSPEP"
     gene            1730..2380
                     /locus_tag="VFH91_07995"
     CDS             1730..2380
                     /locus_tag="VFH91_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6862975.1"
                     /translation="MEYALTATKNEARAGSYYDRIARFYDLTFKLNGYGRSLDQYFAT
                     YPLPVSRGARILDAGCGTGLLTLALLRSIRFPVSITALDLSSTSVAAARKALYYSDGR
                     KRDVTFTQGNLLSLPFADESLDLVVTAGALEYVPLEDGLSELARVIVPGGHLLHLPVH
                     PGVAGVVLEILFRFKSHPPKEVISKTERHFRIVHRYKFPPPQAIGWSKTAILAQKV"
     gene            complement(2387..3526)
                     /locus_tag="VFH91_08000"
     CDS             complement(2387..3526)
                     /locus_tag="VFH91_08000"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6862976.1"
                     /translation="MRKITAFVLTSLLIVTLACDRTPSGETAIADSAPTSATFNIETV
                     ITGLQVPWSIVWAPDGRMFFTERIGRVRVFENGKLRPEPVFTVPDVDPTGESGLMSIA
                     LHPQFNTNHYVYLSYAYSQTGVLVRVVRYKESPEGFIEPKVIIENIPAAQYHAGCRVR
                     FGPDGKLYITTGDGTKRELAQQLNSLAGKTLRLNDDGTVPDDNPFVGRQDARPEIWSY
                     GHRNSQGMDWQPGSNLMFQTEHGPSGFDGPEGGDEVNIVEKGKNYGWPIIHHKESKAG
                     LESPLLEYTPACAPASGMFYRGTVFPQFKGNFFFGCLHGAALMRVELNGRSVVKQESI
                     VKNYGRIRDVAEGPDGFLYFSTSNRDGRGKPSNDDDRILRIVPAK"
     gene            complement(3648..4070)
                     /locus_tag="VFH91_08005"
     CDS             complement(3648..4070)
                     /locus_tag="VFH91_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6862977.1"
                     /translation="MLIRDVIRNREPYSTRASATVQEAAAFMAARNIGAVCVLDEDGR
                     LIGVFSERDVLNRVVVPERDPHNVRVGEVTSELRAVIRCDETPHQALERMERIGTRHL
                     PVVDGERWVGMLSIRDLLRAEVGEQGDEIKLLYEYIQH"
     gene            complement(4331..>5028)
                     /locus_tag="VFH91_08010"
     CDS             complement(4331..>5028)
                     /locus_tag="VFH91_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862978.1"
                     /translation="GDEIVFSFTSDPYVPLESHYKLTRQCLEICAEFHQPIGIITKSA
                     LIRRDIDVLQELSRNASLAVFFTIPFTDVETARAVEPYAPLPDARFHAMQKLADAGIT
                     VGIGIAPVIPGLSSDIPELLKRAKECGAEMAFINMLRLPGSVAPYFEERLRDELPTKA
                     DRVMSRIREARDGKVNSSVFGERMRGKGEYWKATERLFKIHSQRLGFNKKRTHQTAST
                     STFRRPTAQASLF"
BASE COUNT         1199 a         1321 c         1255 g         1253 t
ORIGIN      
        1 caaccttgca aaccaggcaa gaacagagat cgtcgacgaa cgtgagcaac gtgttaacga
       61 ttccattgcc agcatcgcag tgctctatcc ggcgccatat cgagtagcga ttgttaatgc
      121 tgcgaaaact cgaaaaattg atccgcgctt tatcctcgcg ttgattcgtc aggagagtgt
      181 tttcaaacca ttggcaaaat cacctgccgg agcccggggg ttgttacaac tgacaatgga
      241 tgcagcacaa aagtatgccg ccggcgctgg tcttggtcca atcagggaaa atcaacttta
      301 tcaaccagag acctcgattg ttctcggcgc gcagtacttg caaactctct ccgacatgtt
      361 tcccaaacta cttgagcccg ttgcagcgtc atacaatgga ggcgaagata acgtagcccg
      421 ttggctcaag agagggaaac acaacgaccc tggagttttt acctcggaga tcggatttga
      481 cgagaccaag aactatgttc aaaaagtgat gtccaattac cgagtctacc gtcaactgta
      541 cacggctgaa ttgtccccgc gcggttcgtc taaggttcag gactgaatcc tcgagaatct
      601 tctcgtcgcc gcagcggatc gggttcgctt acgggtctcg gtacaactcc taatctccgg
      661 agagccgaat aatattcata gcgtaggttg agctcggttg ccgggcgaga ctcgagttcc
      721 atatttatcc agcgtacgtc attgtctacc gagcgtccga ttccagttgc ggcaaactcg
      781 tcgttgggcg cctgcgatac gacccctcct ttatcacgca tagcgtccga cttagcagac
      841 ggagcgacag gttctccact cctggattcc ttactttgat cgtctcgata aattggtctc
      901 ggcggcgcca gcgttatagc tggacgtttt tctaaaaaga agactgctga gatgacccca
      961 aggttggcag tttggccgag ctttgcaccg tacgaatcac gttcagtcgt gaaataaaat
     1021 cgacgcgctc gtgccgaact catttgccat ccactgatcg tgattgtttg gtaaggttcg
     1081 atgacccact tgctcgcatt ccacgcagtt gtatggctgg cgtcaatcgt attgagtccg
     1141 tcaacagaca gggctacggc aacacgttgt ggaagagggt ttcttattct caactcatac
     1201 tcgacgttag gctgtgcttc aacgtaggtc cttcctctcg cgaagtattc ggtcaggggc
     1261 tggccattca cgagcacttc cagacgaaaa ccttggggta aaggcgggcg ttttgttacg
     1321 gtccgctccg attggacatg attatctgtg gccaacgcag cttctcgtcc gccgaaaatc
     1381 aggacagtcg cgccggttac cccgataaaa accagtgcag caaaagtcag acgaactatc
     1441 atggttgttc acctcccgca gcagtcaaac actttcagaa cgagccatat gcattttctt
     1501 gcaggggaac gagccaaaca gcaagtggga tgtgcggttc ggtaattgct tttgttagat
     1561 taatcaccgg ggaagccggt gaaactgaag gcaagtaagg gccatttggg ctactggtct
     1621 ttttggttga gtaatgcggg ccagaaaagc tacaattacc gcccaatcct ttgagcaata
     1681 aacctgacat ttctttcttg attcggctga gtgaggctta gattagctaa tggagtacgc
     1741 gcttactgca accaaaaacg aagctcgcgc aggttcatat tatgaccgga tagcccggtt
     1801 ttacgacctg actttcaagc taaacggcta cggccgttcg ttggaccagt actttgcaac
     1861 atatccttta ccggtctctc gcggtgcacg aattctggac gccggatgcg gaacgggact
     1921 gctaacgctg gctttgctgc gttcgataag gttccctgtc tcaatcacag cgctggactt
     1981 gtcgtccacc tctgtcgccg ccgcgcgaaa agccctctac tacagcgacg gacgcaagag
     2041 agatgtcacg ttcacacagg gcaatttatt atccctgcca tttgccgatg aatcgcttga
     2101 ccttgtggtc accgccggcg cactcgaata tgttccactt gaggacggac tgtcagagct
     2161 cgcacgcgtg attgttccag gcggacactt actccatctt cctgttcatc ctggagtggc
     2221 gggagtggtg ctcgaaatac tcttccgctt caagagtcac ccaccaaaag aagtcatcag
     2281 caagaccgaa agacatttcc gaatagttca tcgctataag ttcccgccgc ctcaagcaat
     2341 aggctggagt aagaccgcaa tcctggccca aaaagtttag aagactctat ttcgcgggaa
     2401 caatgcgcag aattcggtca tcatcatttg agggtttgcc tcgcccgtcc cggtttgaag
     2461 tagaaaaata gagaaagccg tctggtcctt cggcaacgtc gcggatgcgg ccgtagtttt
     2521 tgacgatgct ttcctgcttg accaccgaac gcccgttcag ctcgactcgc atcaaagcgg
     2581 ccccatgcaa acagccaaag aagaaatttc ctttgaactg cggaaacact gtgccgcgat
     2641 aaaacattcc actggctgga gcgcaggcag gggtgtactc gagcagcggc gactctaaac
     2701 cagctttcga ttccttgtgg tgaataatgg gccagccgta attcttacct ttttctacaa
     2761 tattaacttc atctccgcct tctggaccgt caaagccgct tggaccatgt tcagtctgga
     2821 acatcaggtt gctgccgggt tgccagtcca tcccctggga atttcgatgt ccataggacc
     2881 agatttcagg cctggcatcc tgacggccca caaatggatt gtcgtcaggt acggtaccgt
     2941 cgtcatttag ccgcaatgtt tttcccgcca gggagttcaa ctgctgcgcc agttcccttt
     3001 ttgtgccgtc tccggtagtg atgtagagct ttccatctgg accaaaacgg acacggcaac
     3061 ccgcgtgata ctgtgcggcc ggaatgtttt caatgatcac ctttggctct ataaaacctt
     3121 cgggagattc cttgtagcga acgactcgaa ccagcacacc agtctgggag tacgcatatg
     3181 aaagatagac gtaatgattc gtattgaatt gaggatgcaa cgcaatactc atcagaccgc
     3241 tctctcctgt cgggtcgaca tcaggcacgg taaagacagg ttctggtctc agctttccat
     3301 tttcaaaaac acggacacga ccgattcgct cagtgaaaaa cattcgcccg tcaggcgccc
     3361 agactattga ccatggaacc tgtaagcccg tgataacggt ttcgatattg aatgtcgcgc
     3421 tggtcggtgc agagtctgcg atggccgttt cgccggaagg agttctgtca catgcaagag
     3481 ttacgatcaa taatgaagtt agtacgaatg cagttatttt tctcatgttg ataaagttct
     3541 gaagtagacc ggacttcagc cgagcgctac cgcgacacag tattaacgtt tgctaccact
     3601 atgccaggca atttggtaac aacgaaaagg gctggtttag gtgggggtta atgttgaatg
     3661 tactcgtata gcagtttgat ctcgtcacct tgctcaccga cttcggcacg caagagatcg
     3721 cgaattgaaa gcatcccgac ccaacgctcg ccatcaacta caggaaggtg tcgagtacca
     3781 atcctttcca ttcgttcgag cgcctgatgc ggagtctcgt cgcatcgaat aactgcgcgc
     3841 aactcactgg tgacttctcc gacgcggacg ttatgggggt cgcgttcggg aacaaccacg
     3901 cggtttagga catctcgttc agagaagaca ccgataagtc tgccatcctc gtcgagtaca
     3961 cacacggcgc cgatgtttct ggcagccata aacgcggcag cttcctgcac agttgctgaa
     4021 gctcgggtgg agtaaggctc acggttgcga atcacgtccc tgatcagcat cgcttttact
     4081 ctcctgtcag attccggcct atgaaatacg agacctatga aagtgattgc ggctctctag
     4141 ccttttcttg gcttgtccac tttttgtcca gttgctcgat tgctgtgcct attctcaacc
     4201 attaccgtgc atccccgcaa gatcttcggc gttgtttacc cgcagatacg ccgattctac
     4261 ctcgttcatc gttttgaaaa tcggcgtaat cagcgcaatc tgtggatgat tattggactc
     4321 ccctgtaacc tcagaacaaa ctagcttgag ccgtgggtct gcggaatgtg ctggtggacg
     4381 cggtttggtg agttcgcttc ttgttgaatc ccaaacgctg agaatggatc ttgaacagcc
     4441 gttcagtggc cttccagtac tcccctttgc cgcgcatcct ttccccaaat acactcgaat
     4501 ttactttacc gtcacgagct tctcgtattc ggctcatcac gcgatcagcc tttgtcggaa
     4561 gctcatcgcg taaccgttct tcgaaataag gagcaacact ccctggcaag cgcagcatgt
     4621 ttatgaaggc catttcggct ccgcactctt tggcgcgttt aagcaattcg ggaatatcac
     4681 ttgaaagacc gggaattacg ggagcgatcc cgataccgac cgtgatgcct gcgtctgcaa
     4741 gcttttgcat agcgtggaag cgagcgtcgg gtaatggagc ataaggttcg actgcccggg
     4801 cagtttcaac atctgtaaat ggaattgtaa agaacacagc aaggctggca ttgcgcgata
     4861 attcctgcag aacatcgatg tcacgccgaa tcaaagctga cttagtgatg atcccgatag
     4921 gctggtggaa ctccgcgcag atctccagac attgtcgagt tagcttgtag tgcgactcca
     4981 gtggaacata cggatcagag gtaaaggaaa agactatctc atcacctt
//
LOCUS       DASSFP010000355         6545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1132049, whole genome shotgun sequence.
ACCESSION   DASSFP010000355 DASSFP010000000
VERSION     DASSFP010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132049"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFH91_08015"
     CDS             complement(<1..428)
                     /locus_tag="VFH91_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6862979.1"
                     /translation="MANLQHRLPENVPGDFYVDSSCIDCDTCSQLAPAIYRDHGDQCS
                     VYHQPETDDETKLALMALVACPTGSIGSTRSHDAHIGIDAFPLLMADGVYFCGFTSES
                     SFGGWSYLITRSASKGGNVLIDSPRFATQLVKRIERMNGV"
     gene            complement(623..2014)
                     /locus_tag="VFH91_08020"
     CDS             complement(623..2014)
                     /locus_tag="VFH91_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6862980.1"
                     /translation="MTGAPITKGSILLVDDDESVRNGLFWTLTSDYHVFQAASREAAC
                     AVLSEREIDVVVSDLHLPPHVEDISEGLALIDFARSQDTAPQVVVITGSDSKRAALEA
                     VKRGAYGFFEKPLDTAEIQHIVSQAARMRKLEIENSQLRDELSRPPGFSHLLGTSQAL
                     ERVLKQARSVAAKTATVLLTGENGTGKEMLARAIHQESPRANGPFVAVSCAALPETLI
                     ESELFGHEKGSFTSATQTRKGRFELASGGTLFLDEIGDLSAAVQVKLLRVLQEREFER
                     VGGVKTLTVDIRLVAASNRDLEKEVEEGRFRRDLFYRLNVIPLVLPPLRARLEDIPFL
                     AAHFAAKSAQKHHQPSPELEPALIEVLQEYEWPGNVRELENLIERLVVLSNGGSLGLE
                     FVPEKMLKALPGASKADESTLEGAVGALRRRMIVNALQAENNNKVAAAKRLGISRSYL
                     HRLINEYEIEKKS"
     gene            complement(2142..3989)
                     /locus_tag="VFH91_08025"
     CDS             complement(2142..3989)
                     /locus_tag="VFH91_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6862981.1"
                     /translation="MNARAKAFLVRFAFCLLPLLILTITYHWQQFRVVDSTVNADAQR
                     SLSALSGSLDHRLSETEIALQRFATSAPLREFIKSSEVENESPAREQNAFGFGLPGPL
                     YLDLVVLLKGREHFTSLALFDRKRQPLFKLERQPNGAGGDLLMFHKSGFQPPHSVDVA
                     PNQPVANVVTGPVMQFTVRLTGDNQANETGLLVGELRLDEVIDDLTFSREAQETESAA
                     TSLVIATDNQLRVVYLSNRSTDGQPVNSVLPEFVPIATAAAANRSGLDHLKTADGSVY
                     TTAFSPLPRWHFSLATGRSRTAALTTVHQWGMIGLVLSIGLALIGAVIFGKIFPKRSR
                     GIQRVTEDLSAIVKGELDRRIELKSGDDARGIADRINVVTERLRAQIAREEESRQFES
                     FVRLSAMLTHDLKNAIEALSLIVGNMERHFDNEQFRADAMKSLTGATDKLKGIVARLS
                     RPLTSLSGEHKRPTDVDLVPILKRVVAITAEPQNQKHTIDLRLPDELYALVDATQIEE
                     VIENLVLNALEAMSEPGGTLTVEAGKSANGAVVFSISDTGSGMTQSFVHNRLFRPFST
                     TKKTGVGLGLYTCREAVQANAGSIDVQSIEGLGTTFRVVLPSTATEKRS"
     gene            complement(4011..4862)
                     /locus_tag="VFH91_08030"
     CDS             complement(4011..4862)
                     /locus_tag="VFH91_08030"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET6862982.1"
                     /translation="MSSNRYEYELSVALDLAREAGSLILNFYEGPLEIEQKNNSDDSE
                     PVTQADTLANDLIVSRLAREFPGDGILAEESIDTEKRLDKSRVWMVDPLDGTSGFIAG
                     NGDFAVQIGLVENGVCVVGVVYLPLKNILYRAVVDQGTYIESPGAPVEQRSVSDQKVV
                     SKMRLAASRSHRSPRMNTVVTRLGFKDEIRRGSVGIKIGLIIERKCDVYIHLSPRTKQ
                     WDTCAPQLILTEAGGKLTDLFGAPLSYNVRELRNRNGLVASNGVAHAEIIEMLAPLLD
                     EFGRYPV"
     gene            complement(4887..6389)
                     /locus_tag="VFH91_08035"
     CDS             complement(4887..6389)
                     /locus_tag="VFH91_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET6862983.1"
                     /translation="MDTNELRIKSALDISPEQLNEISLQTSQLVSDYFSGIRDRSVRA
                     GNYAGKTLQAVDTELSQQPVPLETLMNECRSMLDLSRHNGHPRFFGYVASPSTPIGAY
                     ADLVASALNANITCWRSGPAGTEVERLVVRWLGQLISYDDTAAGLLTSGGSMANMIAL
                     LIAARRTLGPEVGNKGLWQGSPLSIYASSEVHMSIAKAADILGFGRDSIRIVDCDERH
                     RIIPAHLRLLIQEDIENGFKPSCVVASAGTVNTGVVDPLDGVADVAREFNLWFHVDGA
                     YGAPGVLDPRKKQLFRGLERADSLSLDPHKWLYVPVDVGCLLYRDPDAASAAFRTEEA
                     DYIKLHGHADDEAFAFWDYGIELSRRFRALKVWFTLRYYGAQQIAQSIVEDNALAKYL
                     GDLVNEAEDFELLAPVELSICCFRYLPRNLVAKNGKTQEPHSLNRALNDFNEKLMSRV
                     QTSGRAYLSNAIVDGNFALRACITNFRTTKSDIEETITVIRELALELQTG"
BASE COUNT         1591 a         1748 c         1606 g         1600 t
ORIGIN      
        1 ttgacgccgt tcatccgctc gattcgcttc actagttgcg tggcaaagcg cggggaatca
       61 ataagcacat taccaccctt tgaagcgctt cttgtaatca agtaactcca gccgccaaaa
      121 cttgactcag aggtgaaccc acaaaagtaa actccgtcag ccattaaaag aggaaatgca
      181 tcgattccga tgtgggcgtc gtggctccgg gttgagccga ttgacccggt gggacaggca
      241 accagggcca ttagcgccag cttagtttcg tcatcagttt caggttgatg atagacgctg
      301 cactggtctc cgtgatcgcg gtagattgcg ggcgctaact ggctacacgt atcgcagtcg
      361 atacagcttg aatcgacata gaaatcaccg ggtacgttct cgggcaagcg atgttgtagg
      421 ttagccatgg atttggtctt tgggcttggc ctttggcctt tggcctttgg tctttggtct
      481 ttggcctttg gcctttggtc tttggccttt ggcctttggc ctttggcctt tggtgtctaa
      541 ttggctaact cacaaattac aaagtccaaa ggtcaaaggt caaagaccta agacctaaga
      601 cctaagacca aagttcaagc ccttaactct tcttttcaat ctcgtattca ttgatcagtc
      661 ggtgcaggta ggatcgactg atacctaaac gttttgctgc ggcaacttta ttattgtttt
      721 ctgcttgtag tgcgttgacg atcattcttc gtcttaaagc tcccacagca ccttcgagcg
      781 tcgattcgtc cgccttactt gcaccgggta aggctttcag cattttttcc ggcacaaact
      841 caagccccag cgatccgcca ttgctcaaaa cgaccaatcg ttcgatcaaa ttctcgagct
      901 cccgcacgtt gcccggccat tcatattcct gcagcacttc aatcaaagct ggttccaact
      961 caggagacgg ctggtgatgt ttttgagcag attttgctgc aaagtgggca gcgagaaatg
     1021 ggatgtcctc tagtcgcgct cttaacggag ggaggaccag aggaatcaca ttcaaccggt
     1081 aaaaaagatc gcggcgaaac cgtccttctt ccacttcctt ctcgagatct cgattactcg
     1141 cggccaccaa acgaatatca acagtcaatg tcttgacgcc accaactcgt tcaaactcgc
     1201 gctcctgcag tactcgcaat agttttacct gcactgccgc gctgaggtct ccaatttcgt
     1261 ccagaaaaag tgtgcctccg cttgccaact cgaaacgtcc cttacgcgtt tgcgtagccg
     1321 aagtaaatga acccttttcg tgaccaaaga gttcagattc gatcaacgtc tcaggcagtg
     1381 cagcgcaact gacggcaacg aatggaccat tcgctcgcgg actctcttga tgaattgccc
     1441 gggcgagcat ctcttttcca gtcccattct caccagtcag caagacggtt gcggttttgg
     1501 ctgccaccga acgtgcctgt ttcaagactc gctcaagcgc ctgactagtt cccagcaggt
     1561 gtgaaaatcc aggaggcctc gaaagctcat cgcgcaattg cgaattctca atctcgagct
     1621 tccgcatcct cgccgcctgg ctgacgatgt gctggatctc tgcggtatcc agaggcttct
     1681 caaaaaatcc gtaagctcca cgtttgactg cttcaagagc ggcgcgcttc gagtcgctgc
     1741 cggtgataac cacaacctgc ggagccgtat cctgacttct tgcaaaatct atcagcgcca
     1801 aaccctcact gatatcttca acatgcggag gaagatggag gtcactgacg acgacatcaa
     1861 tctcgcgttc tgacaagact gcgcacgccg cctcacgcga ggcagcctga aacacgtgat
     1921 agtcagacgt aagtgtccaa aacagtccgt tgcgaaccga ttcatcgtcg tcaacgagca
     1981 gtatggaacc ttttgtgatt ggcgcgccgg tcattctaga ctcgtgatac ctcgatgctg
     2041 aacatccgaa gctccagcgt gagcttaggt aaagttctgg gcaggtactg gggaaccggc
     2101 gtaacgggaa tgccaggtgg aaagagatgc cgcgctcttc tctagctacg cttttcggtt
     2161 gcggttgatg gtagcacgac acgaaaagtc gtaccaagac cctcgataga ttgaacgtcg
     2221 atagaacccg cgttcgcttg cacagcttct ctgcaagtat agagaccgag accaactccg
     2281 gtctttttgg ttgttgagaa agggcgaaat aaacgattgt gaacaaaact ctgggtcata
     2341 cccgatccag tgtcgctaat cgagaacacg acagcgccat tggcgctttt acccgcttca
     2401 acggttaagg tgccacccgg ctctgacatc gcttcaaggg cattgagcac gagattctcg
     2461 atcacttctt caatttgtgt cgcgtcaaca agtgcgtaaa gctcgtcggg cagtcttaaa
     2521 tcgatagtgt gtttctgatt ttggggttcg gcagtaatcg ccacgactcg ctttaagatc
     2581 ggtacgagat cgacgtcagt gggccgcttg tgttcaccgc tcaggctcgt caaaggtctg
     2641 gagagccgcg caacgatccc cttcaacttg tctgtggcac ctgtcaaaga cttcatcgcg
     2701 tcagctcgaa actgttcgtt atcaaagtga cgttccatat tgccgacgat tagagaaagg
     2761 gcctcgatcg cgtttttcaa atcgtgcgtg agcatcgctg agagtcttac gaacgattca
     2821 aactgacgcg attcctcttc gcgggcgatc tgagcacgaa gtcgttcggt cacaacattg
     2881 attctgtcgg caattcctct cgcatcgtca cccgacttga gctcgatccg gcggtccaat
     2941 tctcctttga caatcgctga aagatcttcc gtgacccgtt gaatacctcg cgaacgttta
     3001 ggaaaaattt ttccgaagat aactgcgccg attaacgcta atccaatcga tagcacaagt
     3061 ccgatcattc cccattgatg aactgtagta agcgcagctg ttcgtgagcg accagttgcc
     3121 aatgagaaat gccaacgtgg tagtggggaa aatgctgtcg tgtaaacaga gccgtcagct
     3181 gtcttcaaat gatcgaggcc actcctgttc gccgcagctg ccgtagcgat tggaacaaat
     3241 tcgggtaaca cactgtttac gggttggccg tcagtcgatc tattcgacag ataaactact
     3301 cgcaattgat tgtctgtagc tattacaagt gaagttgcgg cgctttccgt ttcctgcgct
     3361 tcccggctga aagttaggtc atcgattacc tcgtctaacc tcaattcgcc aaccagtagc
     3421 cccgtctcat ttgcttgatt atctccagtt agtctaaccg tgaactgcat gactggaccc
     3481 gtaactacat tagctaccgg ctgattcggt gccacgtcaa cggaatgcgg aggttgaaaa
     3541 ccactcttat gaaacattaa gaggtcgcca ccggctccgt ttggttggcg ctcaagcttg
     3601 aagagcggtt gccttttcct gtcgaacaag gcgagtgacg tgaagtgctc tcgccctttt
     3661 agaagcacta caagatccag atagagcggt ccaggcaaac cgaatccaaa agcgttctgc
     3721 tcccgagccg gtgattcatt ttcaacttca gagcttttta taaactctcg caaaggagca
     3781 gacgtggcga aacgctggag ggcgatctca gtctcactga gccggtggtc caaacttcct
     3841 gataaggcac tcaaactcct ctgagcgtcg gcattaaccg ttgaatcaac aactctaaac
     3901 tgttgccagt ggtaggtgat tgtcagaata aggaggggca gcaggcaaaa agcgaagcgg
     3961 acgaggaatg cttttgctcg agcgttcatt tgattagaag gaaaaaaggt tcaaactgga
     4021 tatcgaccaa actcgtcaag aagtggcgca agcatttcga tgatttcagc gtgggccact
     4081 ccgttgctcg ccaccaggcc gttgcggttc cgcagctctc gcacgttata actcaagggc
     4141 gcaccgaaaa gatctgtcag ctttccaccg gcctcggtca ggataagctg cggcgcacaa
     4201 gtatcccact gctttgtgcg gggagaaaga tggatataga cgtcgcattt acgctcgatg
     4261 attaatccaa tttttattcc gactgagccg cgacgaattt catccttgaa gcccagcctg
     4321 gtaacgacag tgttcatcct cggactacga tgagatctgc tggcagccaa cctcattttc
     4381 gacacaacct tctgatcgga aacactccgc tgctctaccg gagcgcccgg cgattcaatg
     4441 taagtgccct ggtccacaac tgctcgatac agaatattct ttagtggcaa gtaaacgacc
     4501 ccaaccacac aaactccgtt ctcaaccaaa ccgatctgga ccgcgaagtc cccattcccc
     4561 gcgataaagc cactggtacc atcgagtgga tcaaccatcc agacgcgcga tttgtcgagt
     4621 cttttttcgg tgtcaatcga ttcttcggcc aggattccgt cgccgggaaa ctcacgcgca
     4681 agtcgagaaa ctattagatc attggcaagt gtgtcggcct gagtaacggg ttcactatcg
     4741 tcactattgt tcttctgttc gatttccaga ggaccctcgt aaaaatttag aatgagtgaa
     4801 ccagcctcgc gggcgaggtc cagagccaca ctcaattcgt attcgtaacg attagaactc
     4861 atgtctctgc aagcactttt ggaaactcat ccggtttgta attcgagcgc gagttcacga
     4921 atcactgtta ttgtttcctc gatatcactt ttcgtggtgc ggaagtttgt tatacatgca
     4981 cgtaacgcaa aattgccgtc gacgatagcg ttggacaaat aggctcggcc actcgtttgc
     5041 acccgactca ttaatttctc gttgaaatca ttcagagctc gattcagaga atgcggctcc
     5101 tgtgttttac cattttttgc gacaaggttc ctgggtaggt aacgaaaaca acagatgctc
     5161 aattcgacgg gagccagcag ctcaaagtcc tcggcttcat tgacaagatc cccgagatac
     5221 ttcgcaagtg cgttgtcctc aacaatggat tgcgcaatct gctgcgcccc gtagtaacgc
     5281 aatgtgaacc acacttttag tgcccggaac cgtcgagaca actcgatgcc ataatcccaa
     5341 aaggcaaaag cttcgtcatc agcgtgtccg tgaagcttta tgtagtcagc ttcttccgtg
     5401 cgaaacgcag cactcgcagc gtccggatcg cgatagagca agcaaccaac atcgactggg
     5461 acgtacaacc atttgtgagg gtcgagagat agcgaatcag ctcgctcgag acctctgaat
     5521 agctgcttct tgcgcggatc aagtacgccg ggcgcgccat aagccccgtc gacgtgaaac
     5581 cagagattga actcgcgagc tacatccgcc acaccatcga gtggatcgac aacgcccgta
     5641 ttgaccgtgc cggcacttgc gacaacgcac gaaggtttga atccattttc aatgtcttct
     5701 tgtatgagaa gtcgcaagtg ggctggaata attcgatgac gctcgtcgca atcaacaatg
     5761 cggatcgagt cgcgaccgaa gccaagaatg tctgccgctt tggcgatcga catgtgcact
     5821 tcgctcgaag cgtaaatgct caacggagaa ccttgccaaa gccctttatt cccgacctca
     5881 ggtccaagcg tacgacgtgc ggcaatcaag agcgcgatca tgttagccat cgagccgcca
     5941 ctcgtcagta atcccgctgc agtgtcgtca taagaaataa gttggcccag ccagcggacg
     6001 acaagtctct caacttcggt tccggctggt ccagagcgcc aacaggttat atttgcgtta
     6061 agagcagaag cgacaagatc ggcgtacgcc ccaatcggag tggaaggcga agctacataa
     6121 ccaaaaaatc gcgggtgacc gttatggcgg ctcaagtcca acattgaacg acactcgttc
     6181 atgagagttt caagtgggac cggctgctgg ctcaactcag tatcgacggc ctgcagcgtt
     6241 ttgcccgcat aattaccggc gcgtactgag cgatcgcgaa ttccagagaa gtaatcggat
     6301 acgagctgcg aggtttgaag cgaaatctcg ttaagttgct ccggcgaaat gtccaacgca
     6361 gatttgatac gcagctcgtt tgtgtccatg tcgtctattt gttaatcccg ggagcgatgg
     6421 ctacgagcaa cgacagcctt tcagttccaa tattcgttac gccgtgtgga actgtagctg
     6481 gtgcaagcac cagcgtaccg gattccacag tcttttcttc gtcaccaact gtgaaacggc
     6541 cagtg
//
LOCUS       DASSFP010000356         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_841666, whole genome shotgun sequence.
ACCESSION   DASSFP010000356 DASSFP010000000
VERSION     DASSFP010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841666"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFH91_08040"
     CDS             complement(<1..707)
                     /locus_tag="VFH91_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6862984.1"
                     /translation="MGNVGPTKVEPEPQSPSEDVHERERTEALRWKSVFENSAIGVAL
                     TDLDGRFLATNSTYQKMLGYTESEFKSLSFLDITHEEIRDTNRALMAELVEGKRGQFQ
                     IEKQYRRKDGTLFWVRNNVSLVQGSKSMPPAVMALSEDITERKLAEEALQKSEERARL
                     VLNCAAEGIFGCDSHGTCLFCNLAAAHLLGFDTPEELLGKNMHSLEHHTRKDGTAFPI
                     EECPIYMGFLENRGVHV"
     gene            complement(873..1844)
                     /locus_tag="VFH91_08045"
     CDS             complement(873..1844)
                     /locus_tag="VFH91_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6862985.1"
                     /translation="MAEDEKGILGEELQRRSFLKCMAWAGAGVLWVMNAGVLSSCSLL
                     HAGSRRGKFSFVQISDSHIGFNKEPNPDPTKTFQEAVNQINALPDAPAFLIHTGDITH
                     LSKAEQFDTATQVLKGAKAGQVYFTPGEHDVFSDDGKLYLERFGKGTQGNGWQSFDYD
                     GVHFIGLVNVLNLKQGSGTGLGGNGFGQLGQDQLAWLEKDVKGLTSSTPIVVFAHIPL
                     WTIYPDWGWGTEDSAQALSFLKRFGSITVLNGHIHQILQKVEGNVTFHTARSTAFPQP
                     APGTAPAPGPLKDVPADKVRSLLGIAEVTYVDKNSPLALVDSTLAQS"
     gene            complement(2071..2679)
                     /locus_tag="VFH91_08050"
     CDS             complement(2071..2679)
                     /locus_tag="VFH91_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6862986.1"
                     /translation="MTVDPDAQLAATGDSEAFEKVYRRYYHRVYSLCLLMIRNVTQAE
                     DLAQEVFIQLFRKIHTFRGDSSFNTWLHRLTVNHVLMYFRRPIVKTERTTEDGSLPIQ
                     VVRGTANPSRMAVLDRISLREAIRQLPLGYRTVFILHDVQGYEHEEIAQMLGCATGTS
                     KSQLHKARLKLRTILCEQNPPNKKRPFGVQRPAAALLSRCSS"
     gene            complement(2746..3228)
                     /locus_tag="VFH91_08055"
     CDS             complement(2746..3228)
                     /locus_tag="VFH91_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862987.1"
                     /translation="MNEQIMKVLVRTSLLSAMLLVSVVTSTKGQSIEYRIRANIPFDF
                     NVANTKLPAGKYSVERTMQSSGDSILSVSDSNGNSRLSSAAIPVEDWHTSAKARLVFH
                     RYGDQYFLFQVWPPSATTGRQFMKSRSERELMKNVAKNRQNSERDELVQIVTIVGSVE
                     "
     gene            3528..>3955
                     /locus_tag="VFH91_08060"
     CDS             3528..>3955
                     /locus_tag="VFH91_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6862988.1"
                     /translation="MLNRNRQTTLKRTGESTELASFFSRPETQTSRLTSFRLSVIVPA
                     YNERHVVEASLRRVLALEDDLISSLEVIVVDDCSTDGTWPILERLAKEDSRIVLLRNE
                     RNLGKGAALRKAIPCTTGDVTIIHDADLEYDPSDIPSLLVP"
BASE COUNT          955 a         1042 c          931 g         1027 t
ORIGIN      
        1 tcaacatgta cgccacgatt ctcgaggaag cccatataaa ttggacactc ttcaattgga
       61 aaagctgttc catcctttcg agtatggtgt tcaagagaat gcatgttctt cccaagtaac
      121 tcttcgggcg tgtcgaagcc gagcaaatga gcggccgcca gattgcagaa caggcaagta
      181 ccgtgagagt cacatccgaa gatcccttca gccgcacagt tcagaactag ccgtgcgcgc
      241 tcctcacttt tctgcaacgc ttcttcggca agtttgcgtt cggttatgtc ctctgagagt
      301 gccataactg ccggaggcat gctcttagag ccctggacca aagacacgtt gttacgaacc
      361 cagaaaagcg tcccatcttt tcgacgatac tgtttctcga tttgaaactg accgcgttta
      421 ccctcaacca gttcggccat gagggcccga ttcgtatcgc ggatttcttc gtgagtaata
      481 tccagaaacg aaagggactt gaattcactt tccgtgtatc caagcatctt ttgataggtg
      541 gaatttgtgg ccagaaagcg gccatccaga tctgttaagg cgacgccgat cgcagagttt
      601 tcgaagacag acttccagcg cagggcctcc gttctctctc gctcatgtac atcttcggaa
      661 ggagattgag gttcgggttc taccttagtt ggtccaacgt tccccatctg actctcaaca
      721 actccgcctt ttgagcgagt gtgtttcaga gaagattgga agtcaagagg ccctgaaggt
      781 ctgccggatt taaaagcaaa atatattgct tttgagacta tcgacctgat ttgaaccgcg
      841 gtgggtcaac tgttgactgg cacaattgcg aatcatgatt gtgccagcgt tgaatcaacc
      901 agtgccagag ggctgttctt gtcgacgtaa gttacttcag ctatcccaag taagctccgg
      961 accttgtctg ccggtacatc cttcagtggt ccgggcgcag gagcggttcc cggagccggt
     1021 tggggaaacg ccgtagaccg agcggtgtga aacgtgacgt taccttcaac cttttggagt
     1081 atctgatgta tgtgaccatt cagaaccgtg atcgatccga aacgtttcag aaaagagagt
     1141 gcttgcgcgc tatcttcggt gccccaaccc caatcgggat agattgtcca taacggaata
     1201 tgagcgaaca ctacaatcgg cgtgctgctg gttaaaccct taacgtcctt ctccaaccat
     1261 gcaagctggt cctggcccag ttgaccaaaa ccattaccac ccaggccggt gccgcttccc
     1321 tgcttcaagt taagaacatt cacaagacca atgaaatgaa cgccatcgta atcgaagctc
     1381 tgccaaccat ttccctgcgt gcccttgccg aaccgttcga ggtaaagctt tccatcgtct
     1441 gaaaacacat cgtgctcacc cggcgtaaaa tacacctggc ccgcctttgc acccttaaga
     1501 acctgtgtcg ccgtatcgaa ttgttctgcc ttcgaaaggt gtgtgatgtc gcccgtgtgg
     1561 atcagaaatg ccggggcatc tggcaaagcg ttaatttggt taaccgcctc ttgaaatgtt
     1621 ttggtcggat ctggattagg ctctttgtta aagccaatat ggctgtcgct gatctgaaca
     1681 aaactgaact tgcctctgcg actgccagca tgcagcagac tgcaagatga aagaacaccc
     1741 gcgttcatga cccataacac tccggcaccg gcccatgcca tacatttaag aaaacttcgt
     1801 cgctgaagtt cttcccctag aatccctttc tcgtcctctg ccatttttta cctcttttac
     1861 ttaactatga tctgattgag aataagcgca cttctttcat catcgcgcgt cgttatccac
     1921 cctcaagcga cgaatccatc atgcgcttac ctccgagact ggctgcgccc gctaaccagc
     1981 ctcaatttca agtcctgctt gctctctgct ttctataccg aaaaaacgtt cgagatattc
     2041 ccgcatatag ggtgtgccgg cgttttccga ctaactcgaa cagcgcgata acaaagcggc
     2101 ggcgggccgc tgcactccaa aaggcctctt tttgttcggc gggttttgct cgcaaagaat
     2161 agttcgcagc ttcagccgtg ctttgtgtaa ttgagacttg gatgtgccgg ttgcgcagcc
     2221 gagcatttga gcgatctctt catgctcgta gccctgcaca tcatggagaa tgaagaccgt
     2281 gcgataacct aatggcagtt gcctgatcgc ttctctcaaa gaaatccgat cgagcactgc
     2341 cattctgctc gggttcgccg tcccacgtac cacctgaatg ggtagcgagc catcttcagt
     2401 ggtccgttct gtcttaacaa ttggtcttct gaagtacata agcacatggt tcacagtcag
     2461 tctgtgcagc caggtgttaa aggaagagtc gccccggaat gtatggattt tccgaaaaag
     2521 ttggatgaat acttcctggg ccagatcttc agcttgcgtc acgtttcgaa tcatgagcaa
     2581 gcacagcgaa taaacccggt gatagtagcg acgatagacc ttctcgaacg cctcgctgtc
     2641 gccggttgca gctagttgtg catctggatc taccgtcata ccttctcctt taactgacgt
     2701 tgagatcgac gtgactctac tgcgccgctc gagtcacgaa ctctctcatt caacacttcc
     2761 aactattgtt acgatctgga ccaattcgtc gcgctcggaa ttttgccgat ttttcgccac
     2821 atttttcatc aattcacgtt cagaccggga tttcatgaac tgtctgcccg tcgtagcgct
     2881 gggtggccac acttgaaaca ggaagtactg gtcaccataa cgatgaaaca ccaggcgagc
     2941 cttagcactg gtatgccagt cttcaacagg aatagcagcc gaacttaacc gtgaattgcc
     3001 gtttgaatca gatacggaca aaatactgtc acccgagctt tgcatagtcc gctcaacaga
     3061 gtatttaccg gctggaagtt tggtgttggc gacattgaaa tcgaacggaa tattggccct
     3121 gatccgatac tctatggatt gtcctttggt tgatgtcaca acacttacga gcaacatcgc
     3181 actcaggaga ctggttctaa ctaatacttt catgatttgt tcgttcatct ttctcccccc
     3241 acatttaatt ggcgcccaaa acgcctctgt tctagatacc tgacgcggag tagatttatt
     3301 ccatatggca cattaaggtg gtgcttccgt gtaagccata cctcatgagg tctccatctg
     3361 agtctcaagc aatgaccaat ttccagaacg aaaatgttga gaaatgcaaa gccaacagtt
     3421 gtgcttaggc gctcatgcta ttaatctttc agagttttat gggactgaaa ggttcgggtt
     3481 taagtgggtt ttaaattttg caattaagcg gccctccaag ttcgaggatg ttaaatagaa
     3541 accgccaaac aaccctgaaa cgcacagggg aatcaaccga gttagcctcg tttttctcga
     3601 ggcctgagac tcagacctcc agactcacaa gctttcgact tagtgtgatc gtgccggcct
     3661 ataacgaacg gcacgttgtt gaagccagcc tgcgaagggt actcgcgctc gaggatgatc
     3721 taattagcag cctcgaagtt attgtggttg atgactgcag caccgacgga acctggccca
     3781 tcctggagcg gctcgcgaaa gaggatagtc ggatagtatt gctccgtaat gaacggaatc
     3841 tcgggaaagg cgcggcgctc cgtaaagcaa ttccctgcac taccggcgat gtgactatca
     3901 ttcatgacgc cgatctggaa tatgatcctt ccgacattcc ctctctgctg gtgcc
//
LOCUS       DASSFP010000357         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_700282, whole genome shotgun sequence.
ACCESSION   DASSFP010000357 DASSFP010000000
VERSION     DASSFP010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700282"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2540)
                     /locus_tag="VFH91_08065"
     CDS             complement(<1..2540)
                     /locus_tag="VFH91_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET6862989.1"
                     /translation="MTSDKKEEVMLDKITADIRNEVIDSATVSAAAERVWARVSAAAD
                     TTQFELPATVEHIEGCNDFQSLIPDYLEGNLSEARSLLLVDHTHECIPCRKAMKEARA
                     RRMTAPAPARAHRSTLRPVVLRWGIAAALVIGVGLLAVPFVQRYWPMGDLEATVLAAE
                     GQVFQVADTRTATVNAGSKLQAQERIRTAKDGHAIVRLKDGSTIEMKDRSEVYLTKSS
                     QGTTIHLDRGSIVVEAARQHGEHLLVETSDSLVSVTGTVFSVNNGTKGSRVSVVEGQV
                     KLNHAGSDRMLSAGEQATTNPSIAVIPVKDEVAWSRKADQYASLLRGLAALKVDLAKV
                     SSPQVRTSTHLLDLMPANTVVYAAIPNLANNLVESHRLIEERMSQNPALREWWQKEQS
                     GKQNMDQVMQTIRQFGDYLGDEIAVSVSLGANAKPDAPLVLAELKNSNGLREFIEQEI
                     AKYSAGETNKPNIQFVDNPDAAAPVAEGTKSETLFVWIQSDLLAASPRLERLQELAQT
                     VASGGNSGFSANPFHDRIAQIYSEGAGLVVGANLEQIVANTKDERAKGADAEKREATL
                     EKLGILSVKYFILDQKETGGKTHTQASLSFNDTHKGIPSWLAAPGPMGSLEYISADAN
                     VVGGFVVKNPVALVDDLLDVLGTVSPDLRKNLDAQQSSRGLNIRDDIAAPLGGEFAFA
                     IDGPVLPTPAGKMVFEVNDPQHLQQTLERLVGEVNKEAAKFGKSGLVLEHEDLAGRLF
                     YTLKSTEFPLEVNYAYANGYLIVAPTKALVQRALTVHDAGNSVLHSAKFTAGLPADGN
                     ANFSAVLYHNLAPIVQPFANQIANTADQLPQGPQAAVKAMAAEMAPTLA"
     gene            complement(2527..3096)
                     /locus_tag="VFH91_08070"
     CDS             complement(2527..3096)
                     /locus_tag="VFH91_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6862990.1"
                     /translation="MQAAVKHLHLVTDNQPPSQLEKLFQTHHARVFRTAQRVTGSASD
                     AEDVLQTVFLRLVKGQEVYDLSQNPEAYLSRAAINASLDLLRSRNRSRAVGLDEVDAE
                     SIVSRFQSPELEHSDRELQTLVRQAVGRLGKTAAEMFVLRYYEGYDNKEIAVFLNTSP
                     LVVGVVLHRARTKLRKEIGHYLEKHHDKR"
     gene            complement(3261..3986)
                     /locus_tag="VFH91_08075"
     CDS             complement(3261..3986)
                     /locus_tag="VFH91_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862991.1"
                     /translation="MAPHKLMLRYVVLSILLICFDGSVHALKRKPPEGGRVAIVIDDR
                     LAALRASPQLNGTLVRRLGRGRLVAIRAKRESKDGVVFFLVNVTSKTHGWIQREALVS
                     PSQRGDDGRVVKMIESSPSEFEKISLARIFIDYFPRSALRTSVLLLMGDTAEQAAAKL
                     TKGAEKQLARRFNDVSDQSLYLNYSGLDRYNRQGVKFVFDPRGKDLHYDGWAWREIVK
                     AHKRSPELEAARTRLAELESLLR"
BASE COUNT          938 a         1089 c         1014 g         1021 t
ORIGIN      
        1 gcaagagttg gagccatctc cgcagccatc gcttttactg cagcctgtgg gccttgtggc
       61 aactggtcgg cagtattggc gatctgattt gcaaaaggct gaacgattgg agccaggttg
      121 tggtaaagca ccgctgagaa gttcgcattg ccatcagccg gtagaccggc tgtgaatttc
      181 gctgaatgca acacagagtt tccagcgtca tgaactgtca gcgcgcgctg aaccagagcc
      241 ttcgttggtg caacaatcaa gtaaccgttg gcataggcgt aattaacttc cagcggaaac
      301 tcggttgact taagcgtata gaacaaccgt ccggctaagt cctcgtgttc caataccaga
      361 ccactcttgc caaactttgc tgcctctttg ttgacttcac caacaaggcg ttcgagcgtc
      421 tgctgcaggt gttgcggatc attgacctca aagaccatct tccctgcagg agttggcaga
      481 acaggaccat cgattgcgaa ggcgaactct ccaccgagcg gtgcggcgat atcatcgcga
      541 atattcagac cgcgtgatga ctgctgcgcg tcgagattct tgcgcaaatc cggtgacaca
      601 gttccaagga catcaagcag atcgtcaacg agcgcgactg gatttttcac tacaaatcca
      661 ccaactacgt tggcatctgc cgaaatgtat tccagtgatc ccattggacc aggagccgct
      721 agccatgaag gaattccttt gtgggtatcg ttgaaagaga gcgatgcctg agtgtgggtt
      781 ttaccgccag tctctttctg atcgaggatg aaatacttaa cgcttagaat tccaagtttt
      841 tccagggtag cttcgcgctt ttcagcatcg gctccctttg ctcgctcatc tttggtattg
      901 gccacaatct gctccagatt tgcgcctaca accaaaccgg cgccttctga atagatctgc
      961 gcgattcgat catgaaaggg attggcactg aatccgctgt ttccacctga cgcaacggtc
     1021 tgtgccagct cctggagccg ctcaaggcga ggacttgctg cgagtaagtc actctgaatc
     1081 caaacgaaaa gtgtttcact cttagtccct tcagcgacag gcgctgcggc atctgggtta
     1141 tcgacgaact gaatgtttgg cttgttagtt tcgccggctg aatatttggc aatctcttgt
     1201 tcgataaact ctcgcaggcc gtttgagttt ttcaactcgg ccaacacaag aggcgcatct
     1261 ggctttgcgt tcgcacccaa cgacacggaa acagcaatct catcgccaag gtaatcaccg
     1321 aattgccgga tggtctgcat tacctggtcc atgttctgtt ttccggattg ctccttctgc
     1381 caccactcgc gcagagctgg attctgactc attcgctctt cgatgagacg atgtgactcg
     1441 acgaggttgt tggccaggtt gggaattgct gcatagacaa cggtgttcgc cggcataaga
     1501 tcgaggaggt gagtggaggt tcggacttgc ggactcgaaa ctttcgccag atcgactttc
     1561 aatgcggcta aaccgcggag tagtgatgcg tattgatcgg cttttcgact ccatgccact
     1621 tcatctttga caggtatgac ggcaatagaa ggattagtcg tcgcttgttc gcccgcactc
     1681 agcatacgat cactgccggc gtgattgagt ttgacctgac cttcaacaac cgacactcga
     1741 gatccttttg tgccgttgtt gaccgagaaa accgttccgg taacggacac cagagaatcc
     1801 gacgtctcga ctaacaggtg ttcgccatgt tgcctggctg cctcgacgac gatactgcca
     1861 cgatccaggt gaatagtagt tccctggctg cttttcgtga gatagacctc cgagcgatct
     1921 ttcatctcaa tggtcgatcc atctttcagg cggacgatgg catgaccatc cttcgccgtc
     1981 cgaattcgct cttgcgcctg aagttttgat cctgcattga ctgtcgcggt tcgtgtatcg
     2041 gcaacctgga acacttgacc ttcggcggcc agcactgtcg cctcgagatc acccataggc
     2101 caatatcgct ggacaaacgg aacggccaaa agaccgacgc caataaccag tgccgctgct
     2161 attccccagc gtagaaccac cggccgtagg gtggaacgat gggcccgggc tggcgccggc
     2221 gctgtcatac gccgcgctcg agcttccttc atcgctttgc gacacggaat gcactcgtgg
     2281 gtatgatcca cgaggagcag tgatcgagct tcggatagat taccctcgag ataatcgggg
     2341 ataagtgatt gaaaatcgtt gcaaccttcg atgtgctcga cagttgcagg aagttcaaac
     2401 tgggtggtgt ctgccgcagc ggagacacgc gcccaaacac gttctgccgc tgcggagacg
     2461 gtggccgaat caataacttc atttctgatg tcggccgtaa ttttatcgag cattacttcc
     2521 tcttttttat cgcttgtcat ggtgtttctc cagatagtgc ccaatctctt tcctcaattt
     2581 tgtccgcgcc cggtgaagca caacgccaac cacaagaggt gaggtgttga gaaagactgc
     2641 aatctctttg ttgtcgtaac cttcgtagta tcggaggacg aacatctcgg cagccgtttt
     2701 acccagacgc cctactgcct gtctgacaag cgtctgaagt tctcgatctg agtgttccag
     2761 ctcgggactt tgaaagcgac taacaattga ttccgcatca acttcgtcca aaccaaccgc
     2821 tcgagatcga ttacgcgatc tcaataaatc gagcgaagca ttgattgccg cacgagataa
     2881 atacgcttcc ggattttgcg ataaatcgta cacttcctga ccttttacaa gtcgtaggaa
     2941 gactgtttgc agtacgtctt ctgcgtcgga agcgctacca gtgacacgct gcgcggtgcg
     3001 aaacacgcgt gcatgatgtg tttggaagag tttttccagc tggcttgggg gctggttatc
     3061 agtcacgaga tgcaagtgtt tgacggctgc ctgcacgttg ttctccttct ccaacctgaa
     3121 aaacgcggcg ttggagcctt tattaacaac gtgcctcgta aaaattttgg agtgcgacgg
     3181 cccggcgtcg ctttgttata tcgctgcttg attatgtgac aaagcgccgc cgagccggtg
     3241 cactccaaag taataatcag tcatcttaga agactttcca attccgcgag cctggttctg
     3301 gctgcctcca attcaggact cctcttatgt gctttaacga tttcacgcca ggcccacccg
     3361 tcgtaatgaa gatctttgcc tcgaggatca aaaacgaact taaccccctg ccggttatac
     3421 cgatctaatc cagagtagtt cagatagagg ctctggtcag aaacatcatt gaatcgcctg
     3481 gccaactgct tctccgcgcc cttcgtcaac ttcgccgccg cctgctcggc tgtgtcaccc
     3541 atcagcaata aaacactggt gcgcagagcc gagcgcggaa aatagtcgat gaagatgcgg
     3601 gccaaagaaa ttttctcaaa ttccgatggc gaggactcaa tcattttcac tactcgccca
     3661 tcgtctcctc gctgtgacgg ggaaaccaat gcttctcgtt gaatccagcc gtgggttttg
     3721 ctcgttacat taacaaggaa gaaaacaaca ccatccttac tttcccgttt cgcacgaatg
     3781 gccaccaacc ggcctcttcc caggcgccga acaagtgtgc cgttgagttg cggactggct
     3841 ctcagcgcag caagtcgatc gtcaataaca atcgcaactc taccgccctc gggaggcttt
     3901 ctctttaggg cgtggactga accatcgaag caaatcagca ggatcgaaag aacgacgtaa
     3961 cgtagcatca gcttgtgagg agccatacta gtgttcctta aatgaggttt ttctgtgaag
     4021 aatccctaaa acgagatcct cgataatgat caattattaa tt
//
LOCUS       DASSFP010000358         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_251847, whole genome shotgun sequence.
ACCESSION   DASSFP010000358 DASSFP010000000
VERSION     DASSFP010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251847"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1410)
                     /locus_tag="VFH91_08080"
     CDS             complement(118..1410)
                     /locus_tag="VFH91_08080"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6862992.1"
                     /translation="MASMYSTIYVDDIREIKEENPFESMMSRFDRAAQLLDLDPDLYA
                     VLRVPNRELKVYIPTRMDSGRIQVFEGFRVQHNFARGPAKGGIRYAPDVNLDEVRALA
                     AWMTWKCAVVNVPFGGAKGGIICDPQQMSSGELERMTRRYAAELLDFIGPEKDVPAPD
                     MNTNEQTMAWIMDTYSMHARHTVTAVVTGKPIDLGGSSGRREATGRGILFVINEAIKR
                     FKMTPGETRVVVQGSGNVGGIGARLLHEAGYKVVAISDIHGGIYNRNGLNVPEALDYL
                     QATRSFEGYKGVEFVANHELLELECDVLVPAATENQITSQNADKIKCKVLAEGANGPT
                     TAAADEILHKKGVFVIPDILANAGGVTVSYFEWVQDRMGYFWREDVVNERLQDKMVAS
                     FNDLCRYADAHSVDTRTAAYMLAIDRVAYDTRMRGIYA"
     gene            complement(1459..2325)
                     /gene="larE"
                     /locus_tag="VFH91_08085"
     CDS             complement(1459..2325)
                     /gene="larE"
                     /locus_tag="VFH91_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET6862993.1"
                     /translation="MKQSVIQEEPVTVISPEVLAKEVELRKLFRELGSVIVAYSGGVD
                     SSYVAFVANEELKDSALCVTGQSASLPEYQRAEIDDAVERFGFTHEWIQTDELENPSY
                     LANNSNRCYFCKDELYDKLGSFGLDRKVSVIVDGSTMDDLGDFRPGRQAAKQHAVRSP
                     LIEVGLTKSEVRELSKRGGLPTWDKPASPCLSSRIAYGTPVTIERLSKIDRGEEILRE
                     YGFREFRVRHHDTLVRIEIAPDEMDQILNKELMAELSVRFRELGFKFVTLDLQGFRSG
                     SLNEVLPVSIKR"
     gene            2555..>3602
                     /locus_tag="VFH91_08090"
     CDS             2555..>3602
                     /locus_tag="VFH91_08090"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6862994.1"
                     /translation="MLQSENLTSWLIERLLVVASLAIVSAIFPTTAVAQTDDTFGDNG
                     ADPVKLFERAQGAHARGDLEKALELYQQAIKIRPEFPEAEFQVGNILTAQGKYPDAEA
                     AFRRAIELKKSWSLPYSGLGALLMRVNRDADAEKSFRQALIIDANDNLALRLLSEIRL
                     RAGDLKEALELAKRATSAQDAPASAWLTYAMAQRANGDKVTARSILDRLIKDQPDNVA
                     ALLERADLSTDEKNYDAAIGDLKTAAKLKSGDKVVLTRMAYVYQQAGKQEEAAAAAKA
                     AGLPIQTTGADGKIRVIGTTEEIEAANSDDVGVARKALEKLIEKNPENASLLNRLGMT
                     YRTENPNRALELLRR"
BASE COUNT          913 a          978 c          850 g          861 t
ORIGIN      
        1 tcttgagaaa acttgacgat ggtccgagtg ttaaatcatg ccagaccata ctctacctta
       61 aaatggaaat caagcccttt tcacgactta gcgcccattt tgtttatgtt ttttcggtca
      121 cgcgtagatg cctctcatgc gtgtgtcata ggccacgcgg tctatcgcca acatgtacgc
      181 agcggtcctt gtatcgactg aatgagcgtc ggcataacga cacagatcgt tgaaacttgc
      241 gaccattttg tcctgcaatc gttcgttcac aacgtcttcg cgccagaagt agcccatccg
      301 gtcctggacc cattcaaaat aagagactgt gacccctccg gcgtttgcca gaatatccgg
      361 aatcacgaag acgcccttct tgtgaagaat ctcatcggct gcagcggtcg taggaccatt
      421 agcaccttca gcaagcacct tacacttgat cttgtccgca ttctgtgacg tgatctgatt
      481 ctccgtcgca gctgggacca gcacatcgca ctcaagctcc agcagttcgt gattcgccac
      541 aaactccacc cctttgtaac cttcgaatga gcgggtggcc tgtaagtaat ccagcgcttc
      601 aggaacattc aatccgttgc ggttgtaaat tccgccgtga atgtccgata tcgctacaac
      661 cttataaccg gcttcgtgca gcaatctggc accaattcct ccgacattgc ccgatccctg
      721 aacaacgacc cgcgtctctc cgggtgtcat cttgaaacgc ttgatagcct cattgatcac
      781 aaacagaatt ccccggccag tagcctctcg tcttccagat gacccgccga gatcaattgg
      841 cttacctgta accactgccg tgaccgtatg tcgagcgtgc atggaatagg tgtccatgat
      901 ccaggccatg gtctgttcat tggtattcat gtccggagca ggcacatcct tctctggtcc
      961 tatgaagtca agcaattcgg ctgcatacct tctggtcatt cgttcgagct ctccggaaga
     1021 catctgttgg gggtcgcaga taatcccacc ttttgctcca ccgaacggaa cattcacaac
     1081 ggcgcacttc caggtcatcc atgccgctaa agctctgact tcgtccaaat tcacgtccgg
     1141 cgcataacgg atcccgcctt ttgctggtcc acgagcgaag ttgtgctgga ccctgaagcc
     1201 ctcgaacacc tgaatgcgtc ccgagtccat tctcgtaggg atgtagacct ttaattcacg
     1261 attcggaact ctcagcactg catataaatc cggatccaga tcgagcagtt gagcagcgcg
     1321 atcaaaacgc gacatcatcg actcgaaggg gttctcttcc tttatctccc gaatatcatc
     1381 gacatagatg gtgctataca tgctagccat tgagtttctc cattaggcga tatcgtagtc
     1441 agcaaccggg gctcggtttt agcgcttgat cgaaaccggc aacacctcat tcagtgatcc
     1501 gctacggaat ccctgcaaat ctaaggtgac aaatttaaat cccaactcgc gaaaacgaac
     1561 tgacagttca gccatcaatt ctttattaag tatctgatcc atctcatccg gagcgatctc
     1621 aattcggacg agcgtatcgt gatgccggac tctaaactct ctaaaaccat attcacgcag
     1681 aatctcttca ccgcgatcga ttttgctcag tcgttcaatc gtgaccgggg ttccatacgc
     1741 aattctcgat gagaggcatg ggctcgccgg cttgtcccag gtaggcaaac cgccccgctt
     1801 actcaactcg cgcacttccg actttgttag gccaacctcg ataagcggac ttcgaacggc
     1861 atgctgtttc gccgcctgac gtccgggacg aaagtcacca aggtcgtcca ttgttgagcc
     1921 atcaacaatc acgctgacct tccggtcgag accaaaagat cccaacttgt catagagttc
     1981 atctttgcag aagtagcacc ggttggagtt gttggcgaga taactggggt tctctaactc
     2041 gtcggtctga atccattcgt gggtaaatcc gaagcgctct accgcatcat caatctcggc
     2101 tctttgatat tcaggcaagc tcgcagactg gcccgttaca caaagagcgc tatccttcaa
     2161 ttcttcgttc gcaacaaagg cgacgtaact actatcgacg ccacctgaat acgcgacgat
     2221 tacggagccg agctctcgga aaagttttct aagctctact tctttcgcaa ggacttcggg
     2281 cgagatgact gtaacgggtt cctcttggat aactgattgc ttcacgctaa ccaccctcat
     2341 ttaaacagac cagatcttgg ctcttggttg attgcagcga catgagcaaa atgggtgaat
     2401 ggacgcatgg tagcctaagc atgaagggca acgcaacaac ccgtctttga acgcgtaatt
     2461 ggttttcctc attcattttg gaacgctacc taacagcgtg gtaacatgca ttcatcccca
     2521 gacctgacga catacttcaa ctgacgcagg cttgatgctt caaagtgaaa atctcacctc
     2581 atggctgatc gaacgcctcc tggttgtggc gtctttggca atcgtgagcg ccatttttcc
     2641 gaccacagca gtcgctcaga ctgacgatac gttcggggat aacggtgctg atccagtcaa
     2701 actcttcgaa cgagcgcaag gtgctcatgc gcgtggcgat ctcgaaaaag cacttgaact
     2761 ctatcaacaa gcaataaaaa tccgcccgga gtttcccgaa gctgaattcc aggtcgggaa
     2821 tattctcacg gcgcaaggta agtaccctga tgcggaagcg gcatttcgtc gcgcgatcga
     2881 gctgaagaaa tcgtggtcgt tgccttattc cggactgggt gcgttactga tgagagtcaa
     2941 tcgggacgca gatgctgaaa aaagcttccg gcaagctctg ataattgatg cgaacgataa
     3001 ccttgcgttg agattgcttt ctgaaattcg cctgcgtgca ggcgatctaa aggaagcgct
     3061 agagctcgca aagcgtgcta cgtcagctca agacgctccg gcttccgcct ggttgacata
     3121 cgcgatggcc cagcgagcga acggagacaa ggtaaccgcc cgcagtattc tagatcgatt
     3181 aataaaggac caaccggaca atgtggcagc cttactcgag cgcgccgact tatcaactga
     3241 cgagaaaaat tacgatgccg caatagggga tttgaaaacc gcagcgaagc taaaatcggg
     3301 cgacaaggtt gtattgacac gtatggcata tgtttatcag caagccggta aacaggaaga
     3361 agctgccgca gccgctaaag cagccggatt gccgattcaa accacaggcg cagacggaaa
     3421 aattagagta ataggcacca ccgaagagat tgaagcagca aatagtgacg atgtcggagt
     3481 ggctcgaaaa gctcttgaga agctgatcga gaagaatcca gagaatgcga gtcttttaaa
     3541 caggctcggc atgacatatc gtaccgaaaa tccaaaccgc gctttagaac tattgagacg
     3601 cg
//
LOCUS       DASSFP010000359         6929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_543792, whole genome shotgun sequence.
ACCESSION   DASSFP010000359 DASSFP010000000
VERSION     DASSFP010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543792"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFH91_08095"
     CDS             complement(<1..965)
                     /locus_tag="VFH91_08095"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET6862995.1"
                     /translation="MESKAAARICVPVCEASLSNLPAAMDHAAMVADFVELRLDCLDP
                     TELDYFDPETLTQTPKPVILTLRSPDQGGHNRLTETQRLEFWTRWLKHSHDAFLDIES
                     ELLPLIGTASELDCSRVIVSQHDFAGVPDDLVAIYQTMAASPAAIGKIAVTANDVVDC
                     LPIFDLLDRARAGDKKLIAIAMGSAGVMTRILGPSRGGFLTYAALTEDSGTAPGQTTA
                     DDLRSVYHIDTIDTDTFICGIIGMPVMHSVSPHMHNAALVAHGLNGVYLPLEVRSVES
                     FFRRMVRPQTREFDWNLKGLSVTAPHKSAVLKYLDEIDEKAMNIGA"
     gene            complement(1018..1542)
                     /locus_tag="VFH91_08100"
     CDS             complement(1018..1542)
                     /locus_tag="VFH91_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6862996.1"
                     /translation="MFCPKCATQNLDGASFCRSCGANISLVPQALSGQLPQPNEPEIG
                     GSRRGRKHEKPLTLDHAFKSIFMGIAFLLVAIALSRSTGQGWWFWMLIPAFSMMGSGV
                     AQFIRIREREKRAMNTQYNQPVFTERPNASFPNPAQRHPTGELMPPVPSVTEGTTRHL
                     GTEASTRHLDTVDK"
     gene            complement(1543..2331)
                     /gene="rlmB"
                     /locus_tag="VFH91_08105"
     CDS             complement(1543..2331)
                     /gene="rlmB"
                     /locus_tag="VFH91_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET6862997.1"
                     /translation="MSSPGPVNVIYGVNPVLEALRAGKRSIESITIMDGARTERLKEL
                     FQLARDNRIPIHRVNRLVFDQKLGEARHQGVMAQVAAARYTDADDLLDQLSSRINSTD
                     PPLVIGLDGLEDPRNMGSILRTAECSGVHGVFIPERRAVGLTEVVAKTAAGALEYVPV
                     ARVTNLVRLIEQLKERNIWVVGASADAEQSYTEWNWTIPTAVILGNEGHGLHRLVSES
                     CDTLVSIPVSGKLQSLNVSVAAGVLLYEARRQRILAAEKTKGSK"
     gene            complement(2401..3429)
                     /locus_tag="VFH91_08110"
     CDS             complement(2401..3429)
                     /locus_tag="VFH91_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862998.1"
                     /translation="MPKDNENIPPIFLGPMAPSEPQGFKLEEMVTCEECLRANAPTRV
                     ACMYCGQPLPVNEATARLRRPTLKAPERNQPGFNSIIIPQQSVLAEREFAQVAKLLRL
                     DEAALQQIIDANLPLPLARTSTAEESVLVSERLAELGVRAASIGDPELGLTEPHLKVR
                     ALTFQNDALSFHQAGRADVSEVLLSDVMLIVAARIVRSQIEIRERKTRKTEGEIVDSS
                     QFYADELMIDLYSAKDPRAWRIASNSFDFSCLGEYKTLVAGENLNRLTELLRTRMPNA
                     QLDHSYDKLRSILEHVWPSQVETTSTGWKRERPGKYVVGAATSNSNEEQFTRYSRLRS
                     YLVLKPLT"
     gene            3483..4328
                     /locus_tag="VFH91_08115"
     CDS             3483..4328
                     /locus_tag="VFH91_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6862999.1"
                     /translation="MFSHFAPMRIPIFICLIAGTLAITSCAVFRPTDVNGPESNPAPY
                     PILLESEGERQNNALVAWKQMAQHFGLSDQLPEIQPGTATLKNLPANASGGIVLPRVG
                     VGPAETEEELRESLRRFIAEWRPLIGAQLDQLSLVERTDLPAGEKIARYEQHPFRYPL
                     RGDFGQLVIRFRSDRRVTAISSTCLTDVERLQSQLAAQAPKVTSEEAAEHVKGGSLAI
                     SGSSFTVPSTAVVEVRQLVAYVKEQMSSKTALEVHLAWEVELTNAPVKTVYLDAITDE
                     IIATK"
     gene            4421..5149
                     /locus_tag="VFH91_08120"
     CDS             4421..5149
                     /locus_tag="VFH91_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET6863000.1"
                     /translation="MNSFDPTNNVSWQFGHRGRVAVFIDGNNLFHAARFHNIDIDYNK
                     LLRVLLGDGRLLRAFFYTGVDIGAERQQGFLLWMRRNGFRVIQKELKTFYDGTRKANL
                     DVEIAVDMLSLAGRYDTAVLVSGDEDFVYAVNAVAYKGCRVEIAGFRSNTAPKLIDVA
                     DFFIDLGEIADKVRKEVTHGTRYDERDLHDHLPGQYLADQLQMSDPAEEFQSAMRVVI
                     AAEIDEDGAEFDAATEFIRITDEH"
     gene            5165..5542
                     /locus_tag="VFH91_08125"
     CDS             5165..5542
                     /locus_tag="VFH91_08125"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET6863001.1"
                     /translation="MKTFTIQEANELLPEVKSLLKKIQRSHRRLSQFRVKAKKAAEGA
                     DLGGGGLVEGVEYAKRLTELTLETADLESLGVQLKDFERGLVDFPSVRDGRVVLLCWQ
                     LGEGDELEWWHDVDAGFGGRTPI"
     gene            5853..6881
                     /locus_tag="VFH91_08130"
     CDS             5853..6881
                     /locus_tag="VFH91_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET6863002.1"
                     /translation="MSRAEEATDHSAPRKSKLIEFPGANRNSIPEWRKELSERVREVQ
                     ERRAREALETAEAERKETPTTELAPQLELLPPAEVPAMNPLVAAALRRIERAHQPVMS
                     ESRQARAATAVAYAPQLEESEPMQMVQPELLDQLVLDAELHIAEAEAEIATVIEKPHN
                     LVVVPPIEIPELPVRPAPKRLIVDDPNDPALNYLDSIARNVCVDELEHNRASAFRRLV
                     CALLDLLICAALSVPIAFAIEATGNNLREPRTIAVVAGCFILITFFYLTLSTALTGRT
                     LAMRLLSLRVIDRKTGLIPTGGQSIGRSLIYLLSLATAGIGIMFAVVSRDGYTAHDHF
                     TRTAVIIT"
BASE COUNT         1706 a         1792 c         1738 g         1693 t
ORIGIN      
        1 gctccgatgt tcatcgcctt ttcgtcgatc tcgtcaagat attttaagac cgcagacttg
       61 tgaggtgcgg taacacttag cccctttaaa ttccaatcaa attcccgggt ttgcggccgc
      121 accattcttc taaaaaaaga ttcgacactc cggacttcaa gaggaaggta gacaccgttc
      181 aaaccatgag ccactaacgc ggcattgtgc atatgaggcg aaaccgaatg cattactggc
      241 ataccgataa ttccgcagat gaatgtatcg gtatcaatcg tatcgatgtg atatacggac
      301 cgcagatcgt cagcggtagt ttgacctggt gcagttcctg agtcttcggt gagtgcggcg
      361 taggttagaa atccgccacg cgatggacca agtatacgag tcatgacccc cgcacttccc
      421 atggcgatag cgataagctt cttatctccg gctcgcgcgc ggtccaacaa atcaaaaatc
      481 ggaagacagt ccaccacgtc atttgctgtt accgcaatct ttccaatcgc cgcaggtgaa
      541 gcggccatcg tttgataaat cgccaccaga tcatcaggta caccagcaaa gtcgtgctgg
      601 gaaacgatga cgcgcgagca gtcaagctcg gatgccgtgc cgatcaatgg cagtaattcg
      661 gattcgatat cgaggaacgc atcgtgggag tgttttaacc acctggtcca aaactccagt
      721 cgttgggttt cggttaacct gttatggccg ccttgatccg gcgacctcag agtaagaatg
      781 actggcttag gagtctgggt aagcgtttca gggtcgaagt aatccagttc tgtcggatcc
      841 agacaatcga gacgaagttc gacaaaatct gcgaccatcg cggcgtggtc catagctgcg
      901 ggtagattgg acaaactcgc ttcgcatacc gggacacata tccgcgctgc tgctttcgat
      961 tccatggtca cggcctgtcg ccgaagaggg atactcctga gactaaactt tggccttcta
     1021 tttatctact gtatcgagat gcctggtaga tgcctcagtg cccagatgtc gagtagtgcc
     1081 ttcggtcaca ctcggcacag gtggcattaa ttcaccggta ggatgacgtt gcgcaggatt
     1141 aggaaaagag gcatttggtc gttcagtgaa cactggttga ttgtactgag tgttcatcgc
     1201 tcgcttttct cgttcacgaa tccgaatgaa ctgcgcaact cctgagccca tcatcgaaaa
     1261 ggcgggaatg agcatccaga accaccagcc ctggccggta gatctagaga gagcaatggc
     1321 aaccagcaaa aatgcgatcc ccatgaaaat gcttttgaac gcatgatcca gcgtcaaagg
     1381 cttttcatgt tttcttccac gacgagatcc gccgatctcc ggttcgttgg gctgcggaag
     1441 ttgaccactg agagcttgtg gcaccagact gatatttgcg ccacacgatc ggcaaaagct
     1501 ggcgccgtca agattctgcg tagcacattt cggacaaaac atttattttg accctttcgt
     1561 cttttctgcc gccaggatcc tctgtcgccg ggcttcgtaa agaagtacgc cagctgctac
     1621 tgatacgttc aaagattgga gctttccact gaccggaatg cttacaagag tgtcacaact
     1681 ttcactcacc aacctatgta ggccgtggcc ttcgttccca agtatcactg ccgtcggaat
     1741 tgtccaattc cactccgtgt agctttgttc tgcatcagca ctggcaccga cgacccaaat
     1801 gttacgttct ttcaactgct ctatcaatct aaccagatta gtcacgcgag caacgggtac
     1861 atactcgaga gctccggcgg cagtctttgc aacgacttcc gtcagaccaa cagctcgtcg
     1921 ctcagggata aaaacaccat gcacccccga acattcagcg gttctcaata tcgaacccat
     1981 gttccgtgga tcttccaatc catctaatcc aataactaac ggaggatcag ttgagtttat
     2041 tcttgacgac aactggtcga gcagatcgtc agcgtccgtg taacgagctg ccgcaacctg
     2101 ggccatcact ccttgatgtc ttgcctctcc gagtttttga tcaaaaacca atcggttgac
     2161 gcggtgaata ggtattcgat tgtctctggc aagttgaaat aactctttca gccgctcagt
     2221 ccgagcacca tccatgatcg tgatcgactc gattgaccgt ttccctgcac gcagcgcctc
     2281 gagcaccggg ttcactccgt agatgacgtt aaccggaccg ggtgatgaca cactatccct
     2341 ttgccggtcc cttttgggat cttcagggtc gtacctttca ttcttacttc gtttatgcaa
     2401 ttaggttagc ggtttcaaaa ccaaataaga tcgaagacgg gaatatcgtg taaattgctc
     2461 ctcgttactg ttggacgtag cagctccaac cacgtacttg ccggggcgct cacgtttcca
     2521 gcctgttgac gttgtctcga cttgagaggg ccacacatgc tcaagaattg atcgcagctt
     2581 gtcataagag tggtccagct gagcattcgg cattcgcgtt cgcaacagct ccgtcaatcg
     2641 gtttaagttt tctcccgcca cgagtgtttt gtactcacca agacatgaga agtcgaagct
     2701 attactggcg atgcgccagg ccctgggatc ctttgctgaa tagagatcga tcatgagttc
     2761 gtccgcatag aactgactgg aatcaacaat ctcgccctca gtctttctgg tctttcgttc
     2821 ccttatttca atctgactac ggacgattcg tgcagcgaca atcagcatta catcactcaa
     2881 taagacctcg gatacatctg cgcgtccagc ttgatgaaat gaaagggcgt cgttctgaaa
     2941 tgtgagcgcg cgaaccttaa ggtgcggctc tgtaagtcct agttccggat ccccaattga
     3001 agcggcgcga actccgagtt ctgccagtcg ttcactaacc agtaccgatt cctcggccgt
     3061 gctggttcga gccagcggca aaggcaaatt cgcatcgatg atttgctgga gcgctgcttc
     3121 atccaaccga agaagcttcg cgacctgagc gaattccctc tcggccagaa cactttgctg
     3181 cggaatgatg atactgttga atcctggctg atttctctct ggtgctttta atgttgggcg
     3241 tcggagtcgc gcggtggcct cgttaactgg cagtggctgc ccgcagtaca tacaggcgac
     3301 tcgtgttgga gcattagctc gcaggcactc ttcgcaagtt accatctcct ccagcttgaa
     3361 tccctgcggc tcagagggcg ccattggacc aagaaagatc gggggaatgt tttcgttatc
     3421 tttaggcaac agctcctcca attatttgaa ttatacgacg agctgaaact tcggtgtgca
     3481 ctatgttttc acatttcgct cccatgcgaa taccaatctt tatctgctta attgcaggta
     3541 cgctggctat aacctcgtgc gcggtattcc gacctaccga tgtcaacgga ccagaatcaa
     3601 atccagcacc ctatccgatc ctgttggaaa gtgaaggcga acgtcaaaac aatgccctgg
     3661 ttgcgtggaa acaaatggcc cagcactttg gcttaagcga tcagcttccg gaaatccaac
     3721 cgggtacggc aaccctcaag aacctcccgg cgaacgcttc tggaggaatt gtccttccca
     3781 gggtcggagt cggcccagca gagaccgaag aagagctacg agaatcattg cgaaggttca
     3841 tcgcggagtg gcggcctttg attggagccc aactggacca gctgtcgtta gtcgaaagaa
     3901 ctgaccttcc cgctggtgag aagatagcgc gatatgagca gcatccgttc cgttacccgc
     3961 ttcgaggtga cttcggtcaa ctggtcattc gttttagatc ggaccgcaga gtgactgcta
     4021 ttagcagcac ttgtcttacc gatgttgagc gcttgcagtc tcagctggct gctcaggctc
     4081 caaaggttac gtccgaagag gcggccgaac atgtaaaagg agggtcgctg gcaatttctg
     4141 gttccagctt caccgtcccg tccacggctg tcgtggaggt ccgccagcta gtagcgtatg
     4201 ttaaagaaca aatgagttcc aaaaccgcat tggaagtcca cctcgcatgg gaagtagaac
     4261 taacaaatgc ccctgtaaag accgtgtatc tagacgccat tactgacgaa attattgcta
     4321 caaaatgatc tgatccgagt ggaccttgct acttgacgcg cctacggcca aagcagcatt
     4381 ctacgtttag aactcagggt gttatttttt ggagtcatat atgaatagtt ttgatcccac
     4441 taacaatgtt tcgtggcagt tcggacatcg aggtcgagtt gcagttttta tagatggtaa
     4501 caaccttttt cacgcagcac gttttcacaa tatcgatatc gattacaaca agctactgcg
     4561 cgtgctcctt ggagacggac gcttgctccg agccttcttc tatactggag ttgacatcgg
     4621 agccgaaaga caacaaggtt tcctactttg gatgcggcgc aatggctttc gtgtgattca
     4681 aaaagagttg aagacgtttt acgacggcac acggaaagca aatcttgatg tcgagattgc
     4741 ggttgatatg ttgtcgctcg caggtcgcta cgacaccgca gtgctggtct ccggcgacga
     4801 agactttgtc tacgcggtaa atgccgtggc ctacaaaggc tgccgtgtag agatcgcagg
     4861 atttagatcc aatacggcgc caaaactaat cgacgttgcc gacttcttca tagatctcgg
     4921 agagattgcc gacaaggttc gcaaagaagt tacccacggc acgcgttacg acgagcgtga
     4981 tttgcacgac caccttcccg gtcaatacct ggctgaccaa ctgcaaatga gtgacccagc
     5041 tgaagagttt cagtcagcga tgcgagtggt aatcgccgcc gagattgacg aggacggcgc
     5101 tgaattcgac gccgcaacag agtttattag aataacggac gaacactaag ttcaaactat
     5161 agctgtgaaa acatttacga ttcaggaagc caatgaactc cttccggagg tcaagagcct
     5221 gctcaaaaaa attcaaagat ctcatagacg cctctctcag tttcgcgtca aggctaaaaa
     5281 agcagcggaa ggagccgacc ttgggggagg cggcctcgtg gaaggtgtcg aatacgccaa
     5341 acgacttaca gaattgaccc tcgagacagc ggatttagag tcgttgggcg ttcagctcaa
     5401 ggattttgag cgcggtttgg tcgactttcc ttcagtccgc gacggtcgag tcgttctgct
     5461 ttgctggcaa ttgggcgagg gcgacgaact tgaatggtgg catgatgtcg acgccggatt
     5521 cggagggcgc acaccgattt gagacacggt ttgaacgcca gatacctcgc ttgcccctga
     5581 cgacaagttc aagcccttca tcattttctc gttgaccact taaagttatt cctgatagga
     5641 tgcgtcccga tcgcgcaagc gacttggaag cgaaactgta attcagaaac caggtcggtt
     5701 taatcccccg gaggcagttt ccgatgcaat gtacctcgtg cggcgcgccg ttggagtcca
     5761 tcgacttttg tatagactgc ggcgctggaa gtgacgaaaa tttcgaggaa aactgcactc
     5821 cttcatacgg gacagataca caagaatcca caatgagccg ggctgaagaa gctaccgatc
     5881 actcagcacc gcggaaatcc aagctaattg agtttcccgg agcaaatcga aactcaattc
     5941 ccgaatggcg caaagagtta agcgaacgcg tccgggaggt tcaggaacga cgcgcacgcg
     6001 aagcacttga aaccgccgag gccgaacgta aagaaactcc aaccactgaa ttagcgccac
     6061 aactcgaact tctgccgcct gccgaagtcc ctgctatgaa tccgctcgtg gctgcggcac
     6121 tcagaagaat tgaacgcgca caccagccag taatgtcgga aagtcgacag gctcgagcag
     6181 caaccgcagt cgcctacgcg cctcaacttg aggagagcga accaatgcag atggtccaac
     6241 ctgagctgct ggaccagttg gtgttggacg cggaattgca catagccgaa gcagaagcgg
     6301 aaattgcgac cgtaattgaa aagccacaca accttgtagt tgtgccgcct atcgaaattc
     6361 cagaactacc agttagacct gccccaaaga ggttgattgt cgatgatccg aatgatccag
     6421 ccttaaatta tctcgactcg attgccagaa acgtttgcgt tgacgaattg gaacataatc
     6481 gtgcctctgc gtttcggcgt ctggtttgcg ctcttctcga cttgttgatt tgtgcggctt
     6541 taagtgtacc gatagccttt gcaattgaag ctacgggtaa caaccttcgt gagccccgga
     6601 caattgctgt cgtcgccggt tgttttatac tcatcacctt tttctacctc acattatcaa
     6661 ctgcgttgac cggacgcaca ttggcgatgc ggttgttgtc gttaagagtc attgacagga
     6721 agaccggatt aataccaact ggaggacaat caatcggtcg atcattgatt tatttactgt
     6781 cgctcgcaac tgcgggtatc ggaatcatgt ttgcggttgt tagtcgcgat ggctacacag
     6841 ctcatgatca ctttacgagg acagcagtca ttattacttg agagtttgta gagttaaaaa
     6901 atagaaaagc cctgcgatgc ggggctttt
//
LOCUS       DASSFP010000360         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1878780, whole genome shotgun sequence.
ACCESSION   DASSFP010000360 DASSFP010000000
VERSION     DASSFP010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878780"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..555)
                     /locus_tag="VFH91_08135"
     CDS             complement(118..555)
                     /locus_tag="VFH91_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863003.1"
                     /translation="MIKFLSISLLLGSLILAGCQKHTEALESGVAGADETSAIGALRA
                     ILLAERTYSLSNSGEFGTMQQLVDGGFLDSRFSSGRPAKDYIITLNVVAKEAGAPEGS
                     FSCNADPDPTLQRMGRHFFINSTSTTIHANNTQPATESDPSLQ"
     gene            complement(613..1167)
                     /locus_tag="VFH91_08140"
     CDS             complement(613..1167)
                     /locus_tag="VFH91_08140"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET6863004.1"
                     /translation="MTTRCDWARTDLSIAYHDQEWGVPLHDERGLFEFLILEGAQAGL
                     SWETILRKRNNYRKAFDRFNAAKVAKYDDQKIESLMADEGIIRNHLKIQSAIGNAKAF
                     LQVQKEFGSFDAYVWQFVNGKPLQRKRGQPVLARTAVSDALSKDLAKRGFKFVGSTIC
                     YAFMQAVGMVNDHNLSCFRYSELS"
     gene            complement(1183..2163)
                     /locus_tag="VFH91_08145"
     CDS             complement(1183..2163)
                     /locus_tag="VFH91_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6863005.1"
                     /translation="MAPIKIGIVGAGYIGNVHASILGHDDRVAVSAVHDIKSDCAERL
                     ARSCNASVVSTPDQVIEQCDAIFITTPNTKHVDLALKAIDAGIHVFCEKPLATNVTDA
                     TEIFERAKTCRGVFQVGHNRRFAPVYATLKTLITDSHTPHSAHVKMNRGELLKPVWTG
                     DPEVTGGFLYETPIHMFDMMRFLFGEVATLQAIGSNHEYPEVDDFSVLLKFANGFHAT
                     LATAADASWLFPFERVEVFCHHATLVTREMETLVCSTNIAGHFTEQSMQQLPKEEKWG
                     YVQEDRAFIDAIVDGLPPPVTARDGLMSVILVDAVYESIKTNLPVSIALN"
     gene            complement(2172..3014)
                     /locus_tag="VFH91_08150"
     CDS             complement(2172..3014)
                     /locus_tag="VFH91_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6863006.1"
                     /translation="MKLALNGATTMRADLATDLIAAQTAGYDYLEIWAAKLRAFLKQN
                     AIRDLQELFNKHHVPALSINSIEHITFRDELAYQQIKQQCHELCSIAAQINCPYVVVV
                     PGKLPSAGVAKSQILAESVRVLHELADIAATYNVSLGFEFLGQTDCSVQTLAQANEIV
                     KAVDRKNVGLVIDSFHFYAGGSTIEMIDGVAPDSIFIFHLNDSEDLPRQQLEDRHRLF
                     PGLGILPLEEMFRAFRRIGYDRVASIEIFRPEYWEMDPQEVARDARLAAEEVMEKSAS
                     VVAQ"
BASE COUNT          910 a          903 c          877 g          913 t
ORIGIN      
        1 atgtactggc cattcagttc gccggcaata cgcggatccc aatgttcggt aaaggtcgaa
       61 aacttttgct gtagatttac tttctccatg atcgttttcc atgctagttc agaaaaccta
      121 ttgcaacgac ggatcagatt cggtcgccgg ttgggtatta ttggcgtgaa tcgtagtcga
      181 ggttgaatta ataaaaaagt gcctgcccat ccgctgcagc gttgggtcag gatcggcgtt
      241 acatgaaaac gagccttcgg gtgcgccagc ttcttttgca acaacgttca gcgtaatgat
      301 gtagtctttc gccggtcggc cgcttgaaaa tcgcgagtcc aaaaaaccgc catcgacaag
      361 ttgctgcatc gttccaaact cgcctgagtt actcaaactg taggtgcgct cggccaataa
      421 aattgctctc aaagcaccga tagcactcgt ttcatcagca ccagccactc ccgactcgag
      481 cgcttcggta tgcttctgac aaccagccaa aatcagagac cctaaaagca aagatattga
      541 taagaatttg atcatctgtc tcgagctacc tttcaatgtc cgcgctggat atggaagtaa
      601 cgcaacagaa gactaactca attcgctata tctgaaacag gacaaattat gatcgttgac
      661 catgccaact gcctgcatga acgcataaca gatcgtggaa cccacaaact taaaacctct
      721 tttggccagg tctttgctga gagcgtcaga gacagcagtg cgagccagta ctggctggcc
      781 acgctttctt tggagtggtt tgccattaac aaattgccaa acgtatgcgt caaaacttcc
      841 gaactccttt tgcacttgaa ggaaagcttt ggcgttgccg atggccgact ggatcttcaa
      901 gtgattacga ataatacctt catcggccat gagcgactca atcttttgat cgtcgtactt
      961 ggctactttc gccgcgttaa atcgatcgaa ggccttccga tagttgttac gctttctgag
     1021 aattgtctcc cagcttaaac cggcctgggc gccttcaagg atcagaaact caaaaaggcc
     1081 gcgttcgtcg tgaagtggaa caccccactc ttgatcatga taggcaatcg acaaatccgt
     1141 cctggcccaa tcgcaacgag ttgtcatatc tggcacagcc ttctagttta aagcgatact
     1201 caccggcaag tttgttttga tcgattcgta aacagcatcg acaagtatca cggacatcaa
     1261 tccatcacga gcggttaccg gcgggggcaa accgtcgaca attgcgtcaa tgaacgcacg
     1321 atcctcctga acgtaacccc acttttcttc tttgggaagc tgctgcatcg attgctcagt
     1381 gaagtgacca gcgatgttgg tactacagac cagcgtctcc atctctctgg tcaccagggt
     1441 tgcgtggtgg caaaagactt cgactcgctc gaacggaaaa agccaactgg catcggcggc
     1501 cgttgccaga gtcgcatgga atccattggc aaatttcaac agcactgaaa aatcgtccac
     1561 ttccgggtat tcatggttcg aaccgatggc ctgcagagtc gcaacttcgc cgaagagaaa
     1621 gcgcatcatg tcgaacatgt gaatcggcgt ttcataaaga aatcctccgg tcacttccgg
     1681 gtctccagtc caaacaggtt tgagcagttc gcctcgattc atcttgacgt gcgctgagtg
     1741 gggagtatga ctgtcagtga tgagcgtttt caacgttgcg tacaccggag caaaacggcg
     1801 gttgtgaccc acttgaaaaa cgccccgaca ggtttttgct ctttcaaaaa tttcggttgc
     1861 atcggtgacg tttgtcgcta aaggcttttc acaaaacaca tgaatacctg cgtcgattgc
     1921 tttaagagca aggtcaacat gtttggtgtt gggggtagtg ataaagatag cgtcgcattg
     1981 ttcgatcacc tggtccggag ttgaaacaac cgatgcgttg caggaacggg caagacgctc
     2041 agcacaatct gacttgatat cgtgaacggc actgactgcg actctatcgt cgtggccgag
     2101 aatcgacgca tggacgttac cgatgtatcc tgcaccaaca attccaattt ttataggcgc
     2161 catagtttta atcattgcgc aacaactgaa gcgctctttt ccatcacttc ttctgctgca
     2221 agcctggcgt cgcgcgccac ttcctgcgga tccatttccc agtattcggg tcgaaaaatt
     2281 tcgatgcttg caactcgatc gtaaccaatt cgtctgaaag ctcgaaacat ttcctccaga
     2341 ggaagaattc ctaaccccgg aaaaaggcga tgtcggtctt ccagctgctg ccgcggcaag
     2401 tcttcagaat cgttgaggtg gaaaataaat attgagtcgg gagcgacgcc atctatcatc
     2461 tcaatcgtcg atccacctgc gtagaaatga aagctgtcga taactagacc aacattcttg
     2521 cggtccactg ctttcacgat ttcgtttgcc tgggccagcg tctgaacact gcaatcggtt
     2581 tggcccagaa actcaaatcc cagagaaacg ttgtaagtgg cagcgatgtc tgccagttca
     2641 tgcaggacac gcacagattc agccagaatc tggctttttg cgacgcctgc gctcggtaat
     2701 tttccgggca cgacaactac gtagggacaa tttatttgag cggcaatcga gcaaagctcg
     2761 tggcattgct gtttgatctg ttgataagcc aactcgtctc ggaaggtgat gtgttcaatc
     2821 gaattgatac tcagtgcggg tacatgatgt ttgttgaata actcttgcag atcacggatc
     2881 gcattttgtt tgaggaaggc gcgtaatttt gcggcccata tttccagata gtcatagcct
     2941 gcagtttgag cggctatcag atccgtcgca aggtcggcgc gcatggtggt tgcgccgttc
     3001 agtgccagtt tcattgtttt gtgttgcccg cattaacaag attgtggaaa gcaggtgaac
     3061 gcaaaggata gcacgaagcc gggcggttag gtgatagttg cgcttgctgg agaatcgtcc
     3121 agagcgacgt ttcccggcgc ttgaagaatt ccaccaacgg cagttggtgg tcccttaaag
     3181 tcctgcctac aaacacgcct aaccagcttc gcggaattcc accaagggca gttggtggtc
     3241 ccttaaagtc caacctacac acgacaacca accctggctc caagaattcc accaggcagt
     3301 tggtggaatt ccgcggagtt ggttcggcgt gtttgtaggt cggactttaa agaactacca
     3361 actcccgttg gtggaattca aagggaaatt tcgccgtttc ctgcggcggt ttgacttgaa
     3421 tggtgctcta gttgtaaaag cgtcttagct tcctacaatt tgaatttaga tcccttgcaa
     3481 tcccggcatg gaagaccccg ggatagtcag ttttttccga aaggaaagga gataccgatg
     3541 gccctacgat taggagatat agcgcccaat ttcactgcag aaaccactga aggcccaatt
     3601 aac
//
LOCUS       DASSFP010000361         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1731848, whole genome shotgun sequence.
ACCESSION   DASSFP010000361 DASSFP010000000
VERSION     DASSFP010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731848"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1452)
                     /locus_tag="VFH91_08155"
     CDS             complement(103..1452)
                     /locus_tag="VFH91_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6863007.1"
                     /translation="MRQLPCFITTLLLSIALLATPARAQQSREKPRLKDFGSSLKKLK
                     WDPETNRTTEVQNSRTSSNDDDVIKIDTLLVAADLLVLDKQGNTVRGLAANDFSITED
                     GVPQQVGHFLLGDTTGISRSIVLIIDYSGSQFPYIKNSVEAAKVLVDQLKPADQMAIV
                     TDDVQMLIDFTNDKRELKKKLDLLVERSKGSDGFLGIGGTRRRFGNSAQYSALMATLK
                     EAFSKEDQRPIIIFQTDGDEAMYLQNSIVEPSVPPGTPLPLLARVQEEVEQRKKLQRA
                     GRTEFSLDDVYEAVEKSRATIYTIVPGAKLIGLSPAQLVQTRKTEDERAINEMLPTLR
                     KESREAFRQREEARKSLVTDEMIRARADEDVKVQQALAAVAPLAGGWTDFLERPEQAQ
                     TIYSRILSDINQRYIIGYYPVNKLLDGKRHAIKFEVKGHPEYTIVGRKSYYAPSPRQ"
     gene            1833..1908
                     /locus_tag="VFH91_08160"
     tRNA            1833..1908
                     /locus_tag="VFH91_08160"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1866..1868,aa:Ile,seq:cat)
     gene            complement(2052..>3687)
                     /locus_tag="VFH91_08165"
     CDS             complement(2052..>3687)
                     /locus_tag="VFH91_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863008.1"
                     /translation="EKLMQEVWPDTFVEENNLTGNIFALRRAFDHYNFIETVPRRGYR
                     FIADVRQVQVEAISLSGQEVVIKEAIGADKGQINSLAVLPFINASADPNAEYLSDGIT
                     ESIINNLSQLRPLRVMSSSSVARYKNRTHDPQEIGRKLGVRAVLVGRVLQLEDKLIIR
                     TELVDAADGSQLWGTQYDKATRDILEIQEEIAREISETLRLRLSGEEQKLLTRRYTEN
                     IEAYHTYLRGRYYWNKRTPADLQRALGYFWQAVDADPAYALAYAGIADCYNWLGYIFG
                     RMPPQEAKPKAKTAALKALEIDDSLAEAYASLAFVTYVFEWQWKEAQDYFKKAIELNP
                     RYAPVRHVYSVYLATAWGQFDQALAQVNKGLELDPLSLPINHMAAHLLLLARRPDEAI
                     AQFRKTLEIDSRYTLAHQNMGYAFEYKQMYTEAVEEFLQGNPFGDTSAEDMKALEGAF
                     RVSGWRGYLQMQLDLSLARWENEGQWHGYAYSIARNYARLEDRNNAFRWLEAAYKAHS
                     GLLIWTPIELHFDNLRSDPRYQDLLQRLGLPLVGPN"
BASE COUNT          812 a         1008 c          876 g          991 t
ORIGIN      
        1 ttctctactt acgactaacc cctgacccct gacccttgcc cccttcccaa ctgcatgatt
       61 ctggcagtta tccagatggc cgccgtttca ggttcccaca aatcattgtc tgggagacgg
      121 agcgtaatag gactttcggc cgacgatcgt gtattcggga tggcccttga cttcaaattt
      181 gatcgcgtgc ctttttccat ccagcaattt gttcaccgga tagtaaccaa tgatgtaacg
      241 ttgattaatg tcagacaaaa tccgcgaata gattgtttgc gcctgctcgg ggcgttcgag
      301 aaagtcagtc caacctccag ctaatggcgc caccgcagcc agtgcctgtt gtactttcac
      361 atcttcatca gcacgtgcac gtatcatttc atccgtgacc agactctttc gtgcctcttc
      421 acgttggcga aacgcctctc tactctcttt cctcagggtt ggtagcatct cgttgatcgc
      481 acgttcgtct tcggtctttc gcgtctggac gagttgcgct ggacttaggc caatcagttt
      541 tgcgcccggg acgatcgtat agatcgtagc tcgcgacttc tcgactgcct cgtaaacgtc
      601 gtcaagacta aactctgttc tgcctgctcg ttgtaatttc tttctctgtt cgacctcttc
      661 ctgaactctc gctaacaacg gaagtggagt tcccggaggc actgatggct cgacgatcga
      721 attttgcaag tacatagctt cgtcaccatc agtctgaaag atgatgatgg gccgctggtc
      781 ttccttgctg aacgcttctt tgagagttgc catcagagcg ctgtactgtg cgctgtttcc
      841 aaatcgccgc cgagttccgc cgatccccag aaaaccgtcg cttcccttac tcctttcgac
      901 taataagtcg agctttttct tcaactcgcg tttgtcgttt gtgaaatcga taagcatctg
      961 tacatcgtcg gtcactatcg ccatctggtc cgctggtttc agttgatcaa ccaaaacttt
     1021 cgctgcctca acgctgttct tgatgtaagg aaactgactg ccgctgtaat caatgatcag
     1081 aacaattgag cgcgagatgc cagttgtgtc tccaagtagg aagtgaccca cctgctgcgg
     1141 cactccatct tctgtgatac tgaagtcgtt ggcagcaagc ccgcgaactg tgttcccttg
     1201 tttgtcgagt acgagcagat ctgcagcgac cagtaacgta tctatcttga tgacatcgtc
     1261 gtcattactg gacgtgcgcg agttttgtac ttctgtggtc cgattcgttt ccgggtccca
     1321 cttaagcttc ttcaaactcg acccaaagtc ttttagtctg ggcttctcgc gcgattgttg
     1381 ggctctggct ggagttgcga gcagagcaat actgagcagc agtgtagtaa tgaagcaggg
     1441 aagctgacgc atagataggc cgaaacattc aagcgtccgg ggatgtccga aactttaacc
     1501 cgaatgcgtc cagaatcaaa aatcccgcta caatgccacc tcggttgcgg cctgaaataa
     1561 tgtggttccg caacctcgtc gggctcggtg ctcccactgg ctatcttctc aacgcacctt
     1621 cggcgcgagc cacccagatc aaaattccct tatgatcagc cgctactatc gacaccgata
     1681 ctctttcgga ttgattgcct tgatccggcc ccatttgcag catccgactg ggtgcatgtt
     1741 aaactccgct caccgcagag ctcttgacga cgttcgatag aaactcaatc gtacagtcaa
     1801 cggtcggaag ttgttgttcg gcagttcctt gtgcccctgt agctcaacgg ttagagcagc
     1861 agactcataa tctgttggtt cggggttcga atccctgcgg gggcaccaag taacttccca
     1921 ttttgtcgga ctgtgagcgg cgatgattct agttgtgttt tgcgttgacg cgatcagttt
     1981 gcctcttaac cgcgaaacga tctatcaatc ggttattaga gaaatcgcca ggtaaggtct
     2041 caatgaaccg ttcaattggg tccgaccagc ggcagcccaa ggcgctgtag taaatcctga
     2101 taacgtgggt ctgagcggag attatcgaag tgaagttcaa taggcgtcca aatcaacagt
     2161 cccgaatgcg ctttgtacgc cgcttccaac caccgaaagg cattattgcg atcctccaaa
     2221 cgagcgtaat ttcgcgcaat gctgtaagca tatccgtgcc attgcccctc attttcccaa
     2281 cgcgcgagcg acagatcgag ttgcatttgc aaatagcctc tccaacctga aactctaaat
     2341 gctccttcta gcgctttcat gtcttctgcg ctggtatcgc cgaatggatt tccttgcaag
     2401 aactcttcaa ctgcctccgt atacatctgc ttgtactcaa aagcgtagcc catattctgg
     2461 tgtgccagcg tgtaacgtga atctatttca agcgtctttc gaaactgcgc gatagcttca
     2521 tctggccgcc tagccaacaa cagtaagtgg gcggccatgt gattgatcgg gagcgagagg
     2581 ggatcaagct ccaatccctt gttcacttgg gccagagcct gatcgaactg tccccatgct
     2641 gtcgccaaat aaacgctata gacatgtcgc acaggtgcat agcgagggtt cagttcaatg
     2701 gctttcttga agtaatcctg cgcctctttc cattgccact caaacacgta cgtgacgaag
     2761 gctaacgatg cataagcttc cgcgagggag tcatcaattt ccagtgcttt gagcgctgcc
     2821 gttttagcct taggcttcgc ttcctgcgga ggcatgcgcc caaaaatata tccaagccag
     2881 ttatagcaat cggcgatgcc tgcgtaggcg agtgcatagg cagggtccgc gtctacggcc
     2941 tgccaaaaat atccaagcgc gcgttgtaag tccgctggcg tgcgtttatt ccagtaataa
     3001 cgtccgcgca agtacgtgtg atatgcctct atattctcgg tatatcgcct ggtaagcagc
     3061 ttttgttctt cgccgcttag cctgagtcgc aacgtctcag atatttctct ggcaatctct
     3121 tcctggatct ccagaatatc ccgggtggcc ttgtcgtact gtgtacccca aagctgtgac
     3181 ccgtctgcgg cgtccaccaa ttcggtgcgg atgattaact tatcttcgag ttgcaagaca
     3241 cggccgacga gcacggctct aacgccaagt ttgcgaccga tctcctgggg atcatgcgtt
     3301 ctatttttgt agcgagcgac actactactg gacatcactc gtaatggtcg cagttgtgaa
     3361 agattgttga tgatgctctc agtaatccca tcactcagat attcggcgtt cggatctgcg
     3421 ctggcattga tgaagggaag gacggctaga gaattgattt gacctttgtc tgcaccgatc
     3481 gcttctttga tcacaacttc ctgacccgac aagctaatag cttcgacctg cacctgccgc
     3541 acgtcggcga taaaacggta gccacgccgc ggcaccgtct caataaagtt gtagtgatcg
     3601 aaagcccttc ggagagcaaa gatatttccc gtcagattgt tctcctcgac aaaagtatcc
     3661 ggccacacct cctgcataag cttctct
//
LOCUS       DASSFP010000362         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1732664, whole genome shotgun sequence.
ACCESSION   DASSFP010000362 DASSFP010000000
VERSION     DASSFP010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732664"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..986)
                     /locus_tag="VFH91_08170"
     CDS             complement(60..986)
                     /locus_tag="VFH91_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET6863009.1"
                     /translation="MTPNEPSPYATPVSEPATDFSEYQSSQPLQPIPPSHPDAGRSGL
                     ELLIAFGTWIVSILFLLFVPLVLVIPYIIYLFVSGHPPTQATLGTDKGVLFFSILGVI
                     PAHSLTFFMIWLIVTRAGKLPFFKTAGLEWPKTWSPVKWSVVCIVFAVILLAFGLLVT
                     KLYGGGKTQLDLLTESSTQARLATAFIAFATAPLVEELIYRGLLYSALERTLGVAVAV
                     IVVSILFAGVHVLQYINNISVIAVITLLSVSLTLVRAYSGKLLPSVVIHLVFNGVQSV
                     MLAFQPYIEGTDKNVPPAPGFEVLSHLLHRFI"
     gene            complement(1048..1605)
                     /locus_tag="VFH91_08175"
     CDS             complement(1048..1605)
                     /locus_tag="VFH91_08175"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6863010.1"
                     /translation="MDQPKIIDSKTVFRGKVFTVSVDTVQEGDAIYQRDVVHHPGSAV
                     IIPLFPDQTVALVKQYRHPAVRYLLEAPAGTLEERESPEIGAARELEEELGLVARRLE
                     LLSEFFVSPGFCEEKMWVYLATELSNSKQNLDDDEIIQVVKISLEEALEMISDGEIQD
                     AKTIIALLLTASRMKSLNTAEYPAV"
     gene            complement(1853..>3019)
                     /locus_tag="VFH91_08180"
     CDS             complement(1853..>3019)
                     /locus_tag="VFH91_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET6863011.1"
                     /translation="AVMLDSICRAAGIKTGLYTSPHLTSITERILINGTPITEDDFAS
                     YVTGVRETSELLLARGTIEALPTFFEQLTAAALLAFAPSKVSLAILETGLGGRLDATT
                     AAGAEIVAITPIAMDHEQYLGNTIEQIAAEKAAIIRPGVRAVIGEQDPRAFQVILDRC
                     KETNVEPVQTALMPSVVEASAEGRFCVTFQTKENVFERVWLGLLGQHQSQNAATAICV
                     AEILNESGYQISREAIREGVGNARHPGRLELIPGTPSFLLDGAHNPAGALALKRYLDD
                     YARRPLTLIFGAMRDKQLLQMAGILFPIADLVVLTEIDDPRSATAESLATIATNVGVR
                     RVVTTSSSNEALQKAIAETGSSGLICITGSLYLIGELRPRLNLVVTTNRTLGST"
BASE COUNT          751 a          763 c          785 g          720 t
ORIGIN      
        1 gcggcaactg ttaatccgcc gatgccgccg ccaacgacta cgacctcata ggacatagat
       61 cagatgaagc gatgcaggag gtgtgataga acttcgaaac ccggagctgg tggcacattc
      121 ttatccgtgc cttctatgta aggttggaag gccagcatca ccgattgcac gccattaaaa
      181 acaagatgga ttacaaccga cggcagcagt ttcccgctgt aggcacggac aagcgtgagc
      241 gaaacactga gcaatgttat tacagcaatg acggagatat tgttgatgta ttgcaacacg
      301 tgaacaccgg cgaacaggat tgaaacgaca atcacggcta ctgctacgcc gagtgtgcgt
      361 tccagggccg aatagaggag accacgatag ataagttcct ctactaaagg cgctgttgca
      421 aatgcgataa atgcagttgc aagacgcgcc tgcgtcgaac tctcggtaag gagatcgagc
      481 tgtgttttac cgccaccata aagcttcgtc acgagcagtc cgaaggccaa caaaatcacg
      541 gcaaagacga tgcagacgac gctccacttt accggactcc acgtcttggg ccattcgagc
      601 cccgcagtct taaagaatgg tagttttccg gccctggtaa caatcaacca gatcataaag
      661 aaggtcagac tatgtgctgg tataacgccg agaatcgaga agaataagac acccttgtca
      721 gttccaagcg tagcttgagt gggcgggtgt ccagaaacaa ataagtaaat aatataagga
      781 atcactaaaa cgagtggaac gaacagcaga aacaaaatgc ttactatcca ggtgccaaac
      841 gcaatcagga gttctaaccc acttcgcccg gcgtcgggat gagacggggg aatcggttgt
      901 aaaggttgac tggattggta ctcagagaag tccgtggctg gttctgagac cggagttgcg
      961 tatggcgaag gctcgtttgg tgtcactgct tggagtttag gggagttggt ttttatggtc
     1021 aatacgtggg tgcaacggtg aacgggatca cactgctgga tactcggcgg tgttcagcga
     1081 tttcattcgg ctcgctgtca acaacagcgc aataatcgtc ttcgcgtcct gtatctcgcc
     1141 atccgaaatc atttccagcg cttcctcaag cgagattttt accacctgta tgatctcgtc
     1201 gtcatcaagg ttctgtttgc tattggaaag ttctgttgcg aggtaaaccc acatcttttc
     1261 ttcgcaaaag ccgggtgaga caaagaactc cgacaacaat tcgaggcgtc ttgccaccag
     1321 gccaagttct tcttccagtt cgcgcgcggc accgatttcg ggagactctc tttcctcgag
     1381 agtgccagcc ggagcctcaa gcaagtagcg cactgccgga tgtctgtact gcttcacgag
     1441 ggccacagtc tggtccggaa agagtggaat aatcactgca ctacctggat ggtgaactac
     1501 gtcacgctga taaatcgcgt cgccctcttg aaccgtatct actgaaacgg tgaatacctt
     1561 gccacggaag actgtcttgc tatcgatgat ctttggttgg tccatatgaa ttgtccaatc
     1621 tatgctggtc gctcgtccaa tggtagtgac ctaatttgag ctctcctctg tgcaagctct
     1681 gtgtcctctg tgtcttctta gggtggtttt tcccgcagaa cagttaacca caaagacaca
     1741 aggacacaga ggttgcaccg agaaaccaaa ttaagatatc actcgtacaa tagggaagtt
     1801 gacgcgatgc taccgcctgc cgtacagacc agatcccctg attgtccgat cgctaagttg
     1861 acccgagcgt acggttggtg gttacgacca ggttcaaacg aggtcgtagt tcgccgatca
     1921 gatatagcga gccagtgatg caaatcaatc ctgacgatcc cgtctcagca attgctttct
     1981 gaagagcttc gttgctggat gaagtggtca caacccttcg aacgcctacg tttgtcgcga
     2041 tggtcgccag actttccgcc gtcgcgctgc gaggatcatc aatttccgtc agtacaacca
     2101 gatcggcaat gggaaataga atcccagcca tctgaagtaa ctgtttgtca cgcattgcgc
     2161 caaaaataag cgtcaacggt cttcgcgcat agtcgtcgag atacctcttg agcgcgaggg
     2221 cgcctgccgg gttatgagca ccatccagca agaatgatgg ggttcccggg atcaactcga
     2281 gacggccagg atgacgagca ttcccgacac cttcccttat tgcttctcga gaaatctggt
     2341 acccagactc attcaaaatt tctgccacgc agatggccgt cgcggcgttt tgactctgat
     2401 gctggcccag cagcccgagc cacacccgtt caaaaacatt ctcttttgtt tgaaacgtga
     2461 cacagaatcg gccttcggcg cttgcttcaa ctaccgacgg catcagcgca gtttgaacag
     2521 gctcaacatt tgtttcttta caccggtcga ggattacctg gaaagcgcga ggatcttgct
     2581 cgcctatgac tgctcgaact ccaggtcgaa ttatcgctgc cttctctgcg gcaatttgtt
     2641 cgatggtgtt tccgaggtat tgttcgtggt ccatggcgat aggggttatc gcgactattt
     2701 ccgctccagc ggctgtagtt gcatcgagac ggccgcccaa gcctgtttct aagatcgcca
     2761 aactgacttt tgagggagca aacgcaagga gggcagcagc ggtcaattgt tcgaagaatg
     2821 taggaagcgc ttctatcgtt cctcgagcaa ggagtagttc tgacgtctcg cgcacaccag
     2881 ttacgtagct cgcgaaatcg tcttcggtga tgggtgtgcc attgattaag attcgttcag
     2941 taatggaagt taggtgaggc gaggtatata gaccggtttt tattccggca gcccggcaga
     3001 tcgagtccag cattaccgc
//
LOCUS       DASSFP010000363         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1733252, whole genome shotgun sequence.
ACCESSION   DASSFP010000363 DASSFP010000000
VERSION     DASSFP010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733252"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..2126)
                     /locus_tag="VFH91_08185"
     CDS             complement(198..2126)
                     /locus_tag="VFH91_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6863012.1"
                     /translation="MAVRLVKDLLAQTARETFVGRAKELSILTSLLADGPRVILLHGI
                     AGVGKSALLSVFAEKARSYGAAVVALDCRTIEPTERGFLQELDTAIGGPSKTLEQAAQ
                     RLQSLGEPVVLVLDNYEVFRLMDTWLRQVFIPLAPDNVRMIITGRDTPAHAWLTAPGW
                     QRLVRVVPVDSLNEQEAIELLVHHGASEANARVINRFALGHPLALKLAAIVTMEHHST
                     TGLPEFGFQRVFDELIRLYLADVNDPVTRQALDAASVVRRITVSLLHAMLPEVAPQDA
                     FERLKSLPFVEAEHDGLRLHELVQQTIASSVQATDPNGYQEYRRAAWQQLSAEAGRTA
                     PFSFWRYTADLLYMIANPVVREAFFPSDVRRFVVEPAQPEDGPSILEITKLHDSLNNI
                     DSISLWWSHLPQAFHVVRDQTGEVAGFYLMFDPASVDQKVLKQDSITKSWSKHIAVDP
                     VPENQRVLFLRRWLSRDKGEKPGSVQAACWLDAKRIYMELRPMLRRVYLTVNDLQPYA
                     LVAQSLGFQVLNVEKTRSAAMLDFGPASVDGWLAGLGATELGIEADILDTQSHELVLE
                     GQRIKLTKLEFEVFRYLYMRKGTPVSRASLVEDVWGWKHTGSNVIEAVMRTLRKKLGD
                     KSETIETIRGLGYRFRNT"
     gene            complement(2226..2456)
                     /locus_tag="VFH91_08190"
     CDS             complement(2226..2456)
                     /locus_tag="VFH91_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HET6863013.1"
                     /translation="MPLINVKVIEGVFSGAQKEAIVKELTEAMVRIEGENMRQVTWCV
                     VEEVASGSWGVGGTPLTTEAVKALAAGKTATA"
     gene            2863..3087
                     /locus_tag="VFH91_08195"
     CDS             2863..3087
                     /locus_tag="VFH91_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HET6863014.1"
                     /translation="MPFINVKVIEGVFSGAQKQAIVKELTDALVRIEGENMRQVTWCV
                     VEEVQSGSWGVGGTPLTTETVKALAAGNAA"
BASE COUNT          721 a          827 c          806 g          765 t
ORIGIN      
        1 ggtatttgcg cttccacggg tcgcgttttt acgttcagtc atgctgaacc actggtatca
       61 ccaccgcgga cagttgtccg tctatttgcg ggaactcgac attccaatcc cgtcaatcta
      121 tggtccaagt gcggatgaaa acccgttcgt atagaggtct ttggaggttt agcaagtggg
      181 gtggcaaatc ggagccgtca ggtgttccgg aagcggtagc cgagtcctcg gatcgtttcg
      241 atcgtctccg atttgtcgcc caatttcttt cgcagtgttc tcatcactgc ttcgatcaca
      301 ttactgccag tgtgtttcca gccccagacg tcttccacca gtgatgcccg tgaaaccgga
      361 gtacctttgc gcatgtacaa atagcgaaag acctcgaatt ctagtttagt gagttttatt
      421 ctttgacctt ccaacaccag ttcatgggat tgggtgtcca atatgtccgc ttcgatcccg
      481 agttctgtgg ctccgaggcc agcgagccaa ccgtcaaccg acgctggtcc aaaatctaac
      541 atcgcagcgg atctggtttt ttcaacattc agcacctgaa atccgaggct ttgagcaacc
      601 agagcgtacg gctgaaggtc attcactgta agatagaccc gtcgcagcat cggcctcaac
      661 tccatgtaaa tgcgttttgc atccagccaa caggcggcct ggacagaacc gggcttctca
      721 cctttatcgc gactgagcca ccggcgcaga aacaagactc gctgattttc gggaaccgga
      781 tcaacagcaa tatgtttgga ccaggattta gtaatggagt cctgcttcag aactttttgg
      841 tccacggatg ccgggtcgaa catgaggtaa aacccggcga cttcaccagt ctgatcccgc
      901 acaacgtgaa aggcctgcgg tagatgactc caccagagcg atattgagtc aatgttgttt
      961 agcgagtcat ggagtttagt tatctcgaga atagaaggac catcttctgg ttgcgccggc
     1021 tcgaccacga agcgtcgcac gtcgctcgga aaaaatgctt cgcgaacgac cgggttagca
     1081 atcatgtaaa gcagatcggc cgtgtatcgc caaaaactaa atggtgcagt tcgaccagcc
     1141 tcggcgctga gctgttgcca ggcggctcgg cggtactcct gatatccatt tggatcagtt
     1201 gcctgcacag agctcgcaat cgtttgttgg accaattcgt gaagtcgtaa gccatcatgt
     1261 tcggcttcga caaacggcag ggatttcagg cgctcgaatg catcctgggg tgcaacttcg
     1321 ggaagcatcg cgtggagcag cgaaaccgtg atgcgtctga cgaccgatgc tgcgtcaagt
     1381 gcctgtctcg tcactggatc attcacgtcg gccaaataca gtcgtattag ttcgtcgaat
     1441 acgcgctgaa agccaaactc gggcaaacct gtagtgctgt gatgttccat tgtcacaatc
     1501 gccgctagct tcaacgccag tggatggccg agagcaaacc ggttgattac acgtgcgttg
     1561 gcttcgctcg caccgtgatg gaccaacaat tcgatggcct cctgttcgtt gagcgaatca
     1621 acgggcacca ctcgaacgag gcgttgccat cctggagcgg tcagccaggc atgagctggc
     1681 gtatcgcggc cggtgattat cattctcaca ttgtctggag caagtggaat aaagacttgt
     1741 cgcaaccacg tatccatcaa ccggaaaact tcgtagttgt cgagtacaag tacgactggc
     1801 tctccaagcg attgcaaccg ctgcgcagct tgctcgagag tcttgcttgg accgccgatg
     1861 gccgtgtcga gttcctgaag aaagccccgc tccgttggtt cgatggtccg gcaatcgagt
     1921 gctacgaccg ccgcgccgta agaacgtgct ttttccgcaa ataccgacaa caacgcggac
     1981 tttccaactc ctgcaatgcc gtgaagcaat atgacgcgag gtccatcggc gagcagggaa
     2041 gttagtattg aaagttcctt cgctcttccg acaaaagttt ctctcgcagt ctgagccagt
     2101 agatctttta caaggcggac agccatgttt gccattatac ggacggaggc ccgtgtactc
     2161 acagagatgc cggtgaactt ccatctcttt cgggcccccg ttgagacatc ggctgctgct
     2221 actgtctacg cagtcgcagt ctttcctgca gccagtgcct tcaccgcttc ggtggtcagc
     2281 ggagttccgc ctacacccca gcttccactg gccacttctt cgacgacgca ccatgtgact
     2341 tggcgcatgt tttcgccttc gatgcggacc atagcttcgg tcagctcctt gacgatggct
     2401 tctttctgag cacctgagaa aacaccttcg ataactttta cattaattaa cggcatttgt
     2461 ttctttctcc ttcaaaactg gtagtctgag ccctattgct cgaatcggtt tgtgccccgt
     2521 tgggatttac tgtcccaagc ttggcacgag gtgaactata cggctattga accacacaga
     2581 atgaggagaa gatgagaaat ttatgaaatt tttggagtgc ggcggcccgg cgccgctttg
     2641 ttaattagct agatcgttgc aatcgagaat taaaaaagcg gcgccgggcc gccgcactcc
     2701 ataattttca tcaatttctc atcttctcct cattctgcat cgattaaata ccctatagtt
     2761 cgttttcgtg cagcaagctt tggacaagta atccccaaac gccagcacaa gaccgttcga
     2821 gcaatagggc tcgtactatc agttttgaag gagaagaaac aaatgccgtt tatcaatgta
     2881 aaagtcatcg agggtgtttt ctcaggtgct cagaaacaag ccattgtgaa agaactgacg
     2941 gacgcgttag tccgcattga aggcgaaaac atgcgacaag tcacctggtg tgtcgtcgaa
     3001 gaggtgcaaa gtggaagctg gggcgttggt ggcaccccgc taaccaccga gacggtgaag
     3061 gcattggctg ctggaaacgc tgcgtaatca gtcgttgcgc ctatgcccga atggaggcc
//
LOCUS       DASSFP010000364         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1436110, whole genome shotgun sequence.
ACCESSION   DASSFP010000364 DASSFP010000000
VERSION     DASSFP010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436110"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFH91_08200"
     CDS             <1..330
                     /locus_tag="VFH91_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6863015.1"
                     /translation="AEHQLIQEKLADMITELDAARLLVFRAAYLKDTTQGRQTREASE
                     AKLFATEAAWRIVDSAVQIHGGMGLVHGNIVERLYRDVRALRIYEGTSEIQKIVIASQ
                     LFKDQQS"
     gene            327..1475
                     /locus_tag="VFH91_08205"
     CDS             327..1475
                     /locus_tag="VFH91_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6863016.1"
                     /translation="MKLNIVGGGPAGLYFAILMKKQDSSHDITIYERNGPDDTFGWGV
                     VFSGRTLANLREADAESHREITSQFEAWDNVDVVQGDSKISIHGNSFSGIARLRLLKI
                     LQQRAQQLGIGLVFNHEIKDFEALRSSSDLLIGADGVNSALRSFYSQYFVPDLDVRKH
                     RYIWYGTHHLFHGLTLTFRENEAGVFAAHSYKFSRDTSTFIVECDTRTWQRAGFHEMN
                     DDQTREYLRKVFLRDLGDEQLLSNNSKWINFVLVKNCKWHFENVALLGDALHTAHFSI
                     GSGTKLAMEDAIEMAECFQRNNEVSDALAEFERLRRPIIDNYQVAAGESMVWFENAAS
                     YIELPPWDLAYSVMTRSGRVTLDDLRTRDPEFVGQYENSRQPSSTKTH"
     gene            complement(1472..2947)
                     /locus_tag="VFH91_08210"
     CDS             complement(1472..2947)
                     /locus_tag="VFH91_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HET6863017.1"
                     /translation="MWKFSNPIRHQIPPTQWPTAGEASSSMLYSPINIGRRRSISRTW
                     VPAMVPWRATDEGFVTSHNLDWYRRFAQGKPGVLVVEATGVRDIPSGPLLRIGDDRFI
                     PGLRKLVEAVREASEGETLLFIQIIDFLSVRRRPEKDKYFQKFFKVTSQHRTLLRETT
                     QDDNWLTASEKEIRDHLCRSGDEMLDLVLDKRELEALRFGYRERVTDTNFEHIKTLPS
                     ELPPVFAAAARRAREAGFDGVELHYAHAYTMAGFLSALNSRDDGYGGSKENRVRLPLE
                     VFKRVRKEVGHDYVVGVRFLADEVIEGGSRIEDSRYYALEFARAGFDFLSLSKGGKFE
                     DAHQPKVGQAVYPYTGQSGYECMPTVLSDQVGPFGRAVPLVASIKATVNTAGFNVPVV
                     AAGGITTFSQAEEILRSGQADIVGMARQALADPDWFTKVRSGYGDDVRRCTYTNYCEA
                     LDQAHRPVTCKLWDRRDLDEPGVSTVDEGRRRLLPPAWRRS"
     gene            3085..4272
                     /locus_tag="VFH91_08215"
     CDS             3085..4272
                     /locus_tag="VFH91_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6863018.1"
                     /translation="MTDDLLKWRSQFPILDKAVYLISHSLGAMPRGTYEKLHDYAEVW
                     ATRGVRAWAEGWWDMPLTVGDRVARIIGAAPGTVVMHQNVSICQSLILSCFEPSPTRN
                     KIVYSELNFPSVMYVYEAHARNGTLRIETVKSDDGITIPLERMLAAIDETTLLVPISH
                     VLFKSAFLQDARAIINRAHEVGALVVLDTYQSAGTVPFSVTELDTDFATGGSVKWLCG
                     GPGAGYLYVRPDLHEKLEPKTTGWMAHEQPFAFETELRYATGMARFLHGSPAIPALYA
                     AQSGYDIINEIGVERIREKNIRQTNYLIALAEEAGFQVTSPKDSSQRGGTITISHEHA
                     AAIAKELIRREFIIDYRPGAGIRISPHFYTSDEELDLVIKELKKINETDSFHNHETVG
                     AAF"
     gene            4304..>4774
                     /locus_tag="VFH91_08220"
     CDS             4304..>4774
                     /locus_tag="VFH91_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET6863019.1"
                     /translation="MTQQYGDNAPLSYNKYLRVQDLIELQNCLSDPVHHDELLFITVH
                     QAYELWFKQILHELDAAMALLNEDRLTLATRAINRIVDIEKLLINQIHILESMTPISF
                     LSFRDELNPASGFQSMQFREIEFSSGLKDERILREFSSDAYAFGRLQKRFEGDTL"
BASE COUNT         1211 a         1231 c         1166 g         1166 t
ORIGIN      
        1 gccgaacatc aactgatcca ggagaagctt gcggacatga tcacagagct ggacgccgca
       61 cgactccttg ttttccgtgc cgcgtacctg aaggacacaa cccagggacg acaaactcga
      121 gaagccagtg aagctaaact atttgctacc gaggccgcct ggagaattgt cgatagtgcc
      181 gttcagattc atggcgggat gggactcgta cacggcaata tcgttgaaag gctttaccgt
      241 gacgtgagag cgttacgaat ctacgaagga acctctgaga ttcagaagat tgtcatagcc
      301 agtcaacttt ttaaagacca gcaatcatga agctcaatat agtcggtggc gggcctgcag
      361 ggttatattt cgccatcctc atgaaaaaac aagattcctc tcatgacata accatctatg
      421 agcgcaatgg cccggacgac actttcggtt ggggcgtggt tttttccggt aggaccctgg
      481 ctaacttgcg tgaggccgac gcggaatcac acagggaaat caccagtcag ttcgaagcgt
      541 gggacaacgt cgatgtggtc cagggcgatt ccaaaatttc gattcatggc aacagcttct
      601 cgggaattgc aaggcttcga ctcttaaaga ttcttcagca gcgggcacaa caactaggaa
      661 ttgggcttgt tttcaatcac gagattaagg actttgaggc tcttcgcagt tcaagtgatt
      721 tgctgatcgg cgctgacgga gttaacagcg ctctcagatc attctattcg caatacttcg
      781 tgccggatct cgatgttcgt aagcatcggt atatttggta tggaactcac cacctctttc
      841 acggactgac attaactttt cgcgaaaacg aggccggtgt cttcgcagca cactcttata
      901 agtttagtcg tgacacgagc accttcattg ttgagtgcga tacaaggacg tggcagcgcg
      961 ctggttttca tgagatgaac gatgatcaga cgcgcgaata ccttcgcaaa gtcttcctac
     1021 gcgatctcgg cgacgaacag cttctttcca acaattcaaa atggatcaat ttcgttctgg
     1081 taaagaactg taaatggcac ttcgaaaatg ttgcgctgct tggggatgcc ctacacaccg
     1141 cccatttttc gattggctcc ggaaccaagt tggcaatgga ggacgccatt gagatggccg
     1201 agtgttttca gcgcaataac gaagtgagcg atgcgctggc tgagttcgaa cgattgcggc
     1261 ggccaattat cgacaactat caggttgccg cgggagagag catggtctgg ttcgaaaacg
     1321 ccgccagcta tattgaactg cctccgtggg atcttgctta tagcgtcatg acgagaagcg
     1381 gccgagtcac gcttgatgac ttaagaacca gagatccgga atttgttggt caatacgaga
     1441 attccaggca gccatcttca actaaaactc attagcttcg tctccatgcc ggaggcagca
     1501 accgcctgcg accctcatcg actgttgaaa cccccggctc gtcgagatcc cgccgatccc
     1561 ataacttaca cgtgactggt ctgtgggcct gatccaaagc ctcacagtag tttgtgtagg
     1621 tgcatcgcct tacgtcatca ccgtaacccg aacgaacttt tgtaaaccaa tctggatcgg
     1681 caagagcctg tcgggccatg ccaacaatgt cggcctgacc gcttctcaat atttcttcag
     1741 cctgcgaaaa cgtcgttatc ccgccagcag cgaccactgg aacgttgaag cctgccgtat
     1801 ttacagttgc tttgatactc gctaccagcg gaaccgcacg accaaatggt ccaacctggt
     1861 ctgacaagac tgtcggcata cattcgtagc cgctttggcc cgtgtaagga tataccgctt
     1921 gtccgacttt cggctggtgc gcatcttcaa acttgccgcc cttcgaaaga gataaaaaat
     1981 caaagccggc acgtgcaaac tcgagcgcgt agtatcggga atcttctata cgactcccac
     2041 cttctatcac ttcatccgcg aggaaccgga cacccacgac ataatcatgg cccacttcct
     2101 ttcgaacgcg cttaaacact tccaacggaa ggcgaacccg gttctccttc gaaccgccat
     2161 aaccgtcgtc acgactattc agcgcgctca ggaaacctgc cattgtgtac gcatgagcat
     2221 agtgcaactc gacaccatca aacccagctt cgcgagcacg acgtgcagcc gcggcgaata
     2281 cgggcgggag ttcgctcgga agggttttga tgtgttcgaa gtttgtgtcc gtcacccgtt
     2341 cgcggtagcc gaaacgcaac gcttcaagtt cacgcttatc cagtacgagg tccaacatct
     2401 cgtcgcctga tcgacaaagg tgatcgcgaa tttccttttc agaagctgtt aaccaattat
     2461 cgtcttgcgt agtttctctg agtaatgtcc gatgttgaga ggtgaccttg aagaattttt
     2521 gaaaatattt atccttctct ggccggcgtc tcacggaaag aaaatctatg atctggatga
     2581 acagtaaagt ctccccttca ctcgcctcgc gaactgcttc aaccagtttt cgcaagcccg
     2641 gaataaaccg gtcatcgcca atgcgaagca aaggtccgct gggtatgtct cgaaccccgg
     2701 tcgcctcaac cacgaggacg cccggtttgc cttgagcaaa acgacgatac caatccagat
     2761 tatgtgaagt tacaaatcct tcatccgtag ctcgccaggg aaccatggca ggaacccatg
     2821 ttctgctgat tgatcttcgc cgcccaatat ttatcggact gtaaagcatc gaactggatg
     2881 cttcgccggc cgtcggccat tgtgtcggag gtatttggtg ccggatcgga tttgaaaatt
     2941 tccacattgg tttacgttca ctttaacacg ggctgttagg gctggtagag tagcgattta
     3001 gaaccgtacg ccttctctgt agaacggcga ttacgctatg gtacattaac cctggttttc
     3061 agtttctgct ggagggattt ccttttgacc gacgatttac ttaagtggcg aagccagttt
     3121 ccgattctcg acaaagctgt ttacctgatc tcgcatagcc tcggtgcaat gcccagggga
     3181 acatacgaaa aacttcatga ctacgcagaa gtgtgggcca ctcgcggtgt cagagcatgg
     3241 gccgagggct ggtgggacat gccacttacg gttggtgatc gagtcgccag gataattggc
     3301 gccgctccgg gcaccgttgt aatgcatcag aacgtatcga tctgccaatc gttgattctt
     3361 tcttgttttg aaccctcacc aactcgcaac aagatcgttt actcggaact aaatttcccg
     3421 tcggtcatgt acgtctacga ggcccatgca aggaacggaa cactgcggat agaaacagtt
     3481 aagtctgacg acggtattac aattcccctg gagcggatgc ttgccgcaat tgacgaaaca
     3541 actcttctcg ttccaatttc gcacgttctg ttcaagagcg cttttttaca agatgcccgg
     3601 gcaattatta acagggccca tgaagtgggt gctctcgttg tactggatac ttatcaatca
     3661 gcgggcacgg ttccttttag tgtgacggag ctagataccg atttcgcgac cggcggttct
     3721 gtcaaatggc tatgcggtgg gccaggcgct ggatatctat acgtgcggcc cgatttacac
     3781 gaaaaacttg agccgaagac cactggatgg atggcccacg agcaaccatt cgccttcgaa
     3841 accgaactac gttatgcaac tggaatggcc aggttcctac acggatcacc agctattccg
     3901 gctctctacg ccgcgcaatc tggctacgac atcatcaatg aaatcggggt agaaagaatt
     3961 cgagagaaga acataagaca gacgaattat ctgatagctc tcgccgaaga ggctggtttt
     4021 caggtgacta gtccgaaaga ctcttctcag cgaggcggaa ccatcacaat aagtcatgaa
     4081 cacgccgcgg caattgccaa agagcttatc cgtcgcgagt tcatcattga ctaccgaccg
     4141 ggagcaggta ttcggatctc gccccacttc tacaccagcg atgaagagtt agaccttgtt
     4201 attaaagagc tgaaaaagat caacgagaca gattccttcc acaatcacga aactgttggt
     4261 gcggccttct gataagtttc agactgccag aacctgatta atcatgacac agcaatacgg
     4321 tgataacgcg cccctgtctt acaacaaata cctgcgagtt caggatttga tagagctgca
     4381 aaattgtctt tcagatccag ttcatcacga cgaactgctt ttcataaccg ttcatcaggc
     4441 atacgagttg tggtttaaac agatccttca cgaactcgac gcggccatgg cacttctcaa
     4501 tgaagaccga ttgacgctcg caaccagggc aatcaatcgc atagtggaca tcgaaaaact
     4561 gcttatcaat cagattcaca ttcttgaaag catgactccg atcagctttc tctcattcag
     4621 ggacgaactg aatccagcaa gcggttttca atcaatgcag tttcgtgaaa ttgagttttc
     4681 ttcgggccta aaggacgaaa ggattttgcg cgagttttca agtgatgctt acgcgtttgg
     4741 tcgactgcaa aaaaggtttg aaggtgacac tctg
//
LOCUS       DASSFP010000365         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_703695, whole genome shotgun sequence.
ACCESSION   DASSFP010000365 DASSFP010000000
VERSION     DASSFP010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703695"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFH91_08225"
     CDS             complement(<1..590)
                     /locus_tag="VFH91_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6863020.1"
                     /translation="MPAIVKRDALAKFTNDHRQEFEALLRKFVEIPTVSVDPAHANDI
                     KRGVELTVDTIKNFGGTPEVYQADKGNPVVHGVFGKSNGLPSVTVYNHIDVQPASKET
                     EPWNTEPFVFTREGDTYFGRGTTDDKGPALSALYGARAAMEADIPINIRFLWEFEEEI
                     GSPNFEKISTKAAPQLRTDSVVVSDTVWVSRNRPASS"
     gene            1102..2802
                     /locus_tag="VFH91_08230"
     CDS             1102..2802
                     /locus_tag="VFH91_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6863021.1"
                     /translation="MRKRFFAPGILFLVLAFLVPAIIFAQQNRSARRASPPDRSATAT
                     TTTGRPVSGPLIKSDVAEALSVIQANYIDGKKLDYNGIFKSSISGMLSVLDPHSTYFD
                     PVEYASFRTEQRSEYFGIGATIEDLREGSDVNTFIRATFEESPAAKAGLRFGDKIVAV
                     DGQSMKGKTYPEVRKFLLGARGTTVKVTVEHPGNHQSETVNIIRDAVSLPSIAQAYML
                     RPGVGYVAMTGGFNITTADEFEQALKVLHARGMNMLVLDLRGNRGGLLIQAVRVANTF
                     LQRGQMIVSQKGRFRDSSQTYGSVNDSPDGVPVVVLVNGETASAAEIVAGALQDHDRA
                     FIVGETTFGKGLVQFPFQLDYDSALLLTIAKYYTPSGRLIQRDYSNGGFYSYYFPGGL
                     AADKSGQATQQSGPESHTDTGRAVYGGGGISPDEVVKPGRITIAEAHLRDVVFAFSLE
                     LTSGRVPGFDTYKVQRAIDFDHDLAAEDFPVTDALFSQLKKFAASKPVYKATPEQLDR
                     ARTFSERQLRYNVLSAAYGYRTATQVFNDADPQIARGVDAMPRARELAIAASRVRARG
                     "
     gene            complement(2872..>3202)
                     /locus_tag="VFH91_08235"
     CDS             complement(2872..>3202)
                     /locus_tag="VFH91_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6863022.1"
                     /translation="AGYFEANLLALAPTGRLILVGTISGSRSTIDFGVVMSKRLTIRG
                     TVLRGRSNEEKATATRLFSKHVVPLLASGVVKPVVDTVFRLEEIREAHRLMETNQNFG
                     KIVLSLE"
BASE COUNT          755 a          829 c          811 g          807 t
ORIGIN      
        1 gaactcgccg gccgattgcg tgagacccag accgtgtccg acactaccac cgaatcagtg
       61 cgtaattgcg gtgcggcttt ggtgctgatc ttttcgaagt tgggtgagcc aatctcctct
      121 tcaaactccc agaggaagcg aatgttgatt ggaatatcag cttccattgc agctcgagcg
      181 ccgtaaaggg ctgacaacgc cggaccttta tcgtctgtgg tgccccgacc gaaatacgta
      241 tcgccttctc tcgtgaacac aaaaggttcg gtgttccagg gttcagtttc cttcgaggct
      301 ggctgtacat cgatgtggtt gtagaccgta actgacggca gaccgttgct ttttccaaat
      361 acgccatgta ccaccggatt acctttgtcg gcctgataaa cctcgggggt acctccgaag
      421 tttttaatcg tgtcgacggt tagttcaacg cctcgtttta tgtcgttcgc gtgtgccgga
      481 tcgaccgaga cagtcgggat ttcaacaaac ttgcggagta gtgcttcgaa ttcctggcga
      541 tgatcgttgg taaacttagc taaggcgtcc ctctttacta ttgcgggcat taaggatcct
      601 ccggcgactt gtcgtgcgaa aactcgctca taatatacga gttaggtacc aaaacaataa
      661 cggctgaatg gagcgactgc gcaagattta ctcgtttact ggtcgtgcgg tcattagtgc
      721 cgaagatcct ttggcacagc tcgcaacagc gcgtcgtccc ctcgaaaagc tggcgttaat
      781 tcgagctcaa tacgggcgac caaaagagct ttggtggcat ctacacaaaa gcgcgagttc
      841 caaatctgac taactgcctg aatttcttcg attcagccat gatttttagg tggtttttaa
      901 gtccaggggc tagatttctg aaactgaagg tgatttctag agcggtttct cgtccttagg
      961 ctgcacgcga aaaacttaac tacagtctcg agcgtacatt caaaagcccc cacgcttcgt
     1021 gttatgctgg cttcccgttt ttgcccgaag ctacggccaa ccaagcgggc acaaagaaca
     1081 gatagttttt aggaggtttg cgtgagaaaa agattttttg ccccgggcat tcttttcctt
     1141 gttttagctt ttttagttcc cgcaattatc ttcgcccagc aaaatcggtc agcacgaaga
     1201 gcaagcccac ctgaccggag tgccaccgct acaactacta ccggcaggcc tgtttcaggt
     1261 cctctcatca agagtgatgt ggcagaggcg ctttctgtga tccaggcaaa ctacatcgac
     1321 ggaaagaagt tggattacaa cgggatcttc aagtcgtcga tcagcggcat gttgagcgta
     1381 cttgacccgc actcgactta ttttgatccg gttgaatacg cgtcctttcg aactgagcag
     1441 cgctcggagt actttggtat cggagctacc atcgaagatc ttcgtgaggg ctctgatgta
     1501 aacacattca tccgtgccac cttcgaggaa tctccagctg ctaaagccgg ccttcgcttc
     1561 ggggataaga ttgtggcagt tgacggccag tcgatgaagg gcaagaccta ccctgaagtt
     1621 cgtaagtttt tacttggcgc acgtggtacc accgttaaag tcactgtcga acatcccggc
     1681 aaccatcaga gcgagaccgt caatatcatt cgcgacgcag tttcgctccc gtctatcgca
     1741 caagcctaca tgctgcgacc gggtgttggt tatgtcgcca tgacgggcgg tttcaatatc
     1801 acgacagcag atgagtttga acaggctttg aaggtgcttc acgctcgggg catgaacatg
     1861 ttggttctcg atcttcgcgg aaatcgtggg ggcttgttga tacaggcagt tcgggtcgcc
     1921 aatacttttc ttcaacgtgg acagatgatc gtctctcaaa agggtcgatt ccgcgacagt
     1981 tcacaaacct atggctccgt taatgacagt cctgatggcg tgccggtggt cgttctggta
     2041 aacggtgaga cagcctcagc ggcggaaatc gtagcgggcg ccttacagga tcatgatcga
     2101 gcattcattg tcggcgaaac gacattcgga aaaggcctgg tccaattccc cttccaattg
     2161 gattacgact cggctttgtt gttgaccatc gctaagtact acacgccatc tggacgcttg
     2221 atccaacgcg attactcgaa tggcggtttc tatagctatt acttccccgg cggtttagcg
     2281 gcggataaaa gcggccaggc aacacagcaa agcggccccg agagtcatac ggacaccggt
     2341 cgagccgtat atggtggggg cggcatttca ccggatgaag ttgtaaagcc tggacggata
     2401 actattgcgg aagcacatct ccgtgatgtg gtttttgctt tttcactcga actaacaagt
     2461 ggccgagttc ccgggttcga tacctacaaa gttcagcgag ccatcgattt tgatcacgat
     2521 ctcgcagccg aagattttcc ggttactgat gcgttgttca gtcaattgaa gaagtttgca
     2581 gccagcaaac cagtctacaa agcgacgcct gaacagctgg atcgagcgcg aactttttct
     2641 gaaagacaac tgcgctacaa cgttttatca gccgcttatg gttatcgaac cgcgacacag
     2701 gtctttaatg atgccgaccc gcagatcgct cgcggcgtag atgcaatgcc tcgtgcgcga
     2761 gaattggcaa ttgccgccag tcgcgtacgc gcgagaggtt aaaaatcagc gggtgagtct
     2821 cgcaggtcga gactcacccg ctgcaactta acccggcctc tacaacccac tctattccag
     2881 cgacagtaca atctttccaa agttctgatt cgtctccatt agccggtgtg cttcccgtat
     2941 ttcctccagc cggaaaacag tatccacgac tggtttgaca acgcccgaag caagtagtgg
     3001 aaccacatgc ttagaaaaga gtcgagttgc cgtcgccttc tcttcattcg agcggcccct
     3061 caaaactgta cctcgaattg ttaggcgttt cgacatcacg acaccaaaat caatggttga
     3121 tctggagcct gaaattgtac caaccagtat caatcgtcca gtcggcgcta acgctaataa
     3181 gttagcttca aaatagcccg cg
//
LOCUS       DASSFP010000366         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2192480, whole genome shotgun sequence.
ACCESSION   DASSFP010000366 DASSFP010000000
VERSION     DASSFP010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192480"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..847)
                     /locus_tag="VFH91_08240"
     CDS             complement(221..847)
                     /locus_tag="VFH91_08240"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HET6863023.1"
                     /translation="MASPAKKSIYSVHPGVLMVQKWVTELKTKTGRSLEEWIRYIKKN
                     GPKGEKERREWLKTEHGLGTNTAGWLAERTEGKGAETADPDLYLKSAEQDVEKMFAGK
                     KAALRPIYDALLKLGLKVGKDAKACPCQTIVPLYRNHVFAQIKPTTQTRIDLGFALGD
                     MKPTRRLIDTGGFAKKDRITHRIPITSLDDIDDEVTHWLRVAYDRDTK"
     gene            complement(889..2505)
                     /locus_tag="VFH91_08245"
     CDS             complement(889..2505)
                     /locus_tag="VFH91_08245"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6863024.1"
                     /translation="MKRRQFLKATTATCALAIVTPVSPATTSETSKPTFPQPRAFELD
                     EMTIQQLQQGMQSGKFSSRSLVEKYSDRINDVDKRGPAINSVIELNPEAESIATRLDQ
                     ERKAGRVRGPLHGVPILIKDNIDTTDRMMTTAGSLALVGAKPTRDSFVAEKLRAAGAV
                     ILGKTNLSEWANFRSNHSSSGWSGRGGQTKNPYVLDRNPCGSSSGSGAAVAANLCTAA
                     IGTETDGSVVCPSSANSLVGIKPTVGLVSRAGIIPISHSQDTAGPMARTVADAAAILG
                     TLTGVDPRDPETRNSSRGTRTDYTTFLDVNGLKGVRLGIARKHFGFSDQVDKLMSDLL
                     AEMKKQGAVLVDPADIPTTGKFDGSELEVLLYEFKADLNTYLAGLGPQAPVHNLKEVI
                     DFNERNRAKEMPYFGQDIMIKAQEKGPLTSKAYLAALRKNHLLTRTQGIDFIMKQHRL
                     DALVAPTGGPSWPTDWVNGDHFTGGYSSASAIAGYPHLTVPAGYIHGLPVGISFFGGA
                     YSEPKLISIAYAFEQATRARQLPKFLETAF"
     gene            complement(2557..3105)
                     /locus_tag="VFH91_08250"
     CDS             complement(2557..3105)
                     /locus_tag="VFH91_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863025.1"
                     /translation="MSRILVAFLLATVTGSLTLAQNSSHSKTRLERDSWITFTSDVGR
                     FSVKMPEAPSSRSETIESERGPYSTHTFTASTPQSAFYVAWGEYDPSYNFYFVQPELI
                     LNRDNFVNGISGTLLTSKNLTIDGYQSLEFTAETAKREYKGRIYMVGRRPYLVVVGTY
                     KKENDSINVNRFFESFKVKIAE"
     gene            3248..>3385
                     /locus_tag="VFH91_08255"
     CDS             3248..>3385
                     /locus_tag="VFH91_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET6863026.1"
                     /translation="MNSPEPTQAPRPRRLVAIVGRPNVGKSTLFNRLIGERRAIVGDE
                     PG"
BASE COUNT          746 a          854 c          870 g          915 t
ORIGIN      
        1 gatcactcgc tatttatcac attctggact ggttcggtga ttatggcggt ggcctgtttg
       61 cttgctttgt atttttcgcg cgcccacgcc cgtgattatc gtgatgccgg agtggccgag
      121 cccgcgtagg ttggtcagta ccgtagcagg cggtagcttc gggtgagttc ccaatcaaca
      181 aacaattgag aattatcaat tcataattga tcgatagttc ctatttggtg tcacggtcgt
      241 aagccactct cagccagtgt gttacttcgt cgtcaatgtc atcgagtgag gtgattggta
      301 ttcgatgcgt gattcggtcc tttttggcga atcctccggt atcaatcaag cgcctcgtag
      361 gtttcatgtc gccgagtgcg aatcctaaat caatgcgtgt ttgcgtggtg ggtttgatct
      421 gggcaaagac gtgattgcga tagagtggaa cgatagtttg acaggggcag gccttggcgt
      481 cttttccgac ctttaggcca agcttcagca atgcgtcata gattggtcta agtgctgcct
      541 tctttccagc aaacatcttc tcgacatctt gttcggcaga tttcagatag agatctgggt
      601 ccgcggtttc cgcacctttt ccttcagtgc gctcagctag ccaccctgca gtgtttgtcc
      661 ccagaccgtg ttcagtcttc aaccattcac gccgttcttt ttcgcctttc ggaccattct
      721 tcttgatgta tctgatccat tcttcaagtg agcggccggt tttagtcttt agttcggtca
      781 cccacttttg gaccatcagc actccggggt gaacactgta gattgatttc ttagctggac
      841 tagccatata gcattccttt ctttaggggt tgtactttgc acgaaccctc aaaaagctgt
      901 ttccaaaaac ttcgggagtt ggcgcgcgcg ggtggcttgc tcgaatgcgt acgcaatact
      961 tatcagtttc ggttcagaat acgctccacc aaaaaatgaa atgccaactg gtagaccgtg
     1021 aatatacccg gcgggtaccg taaggtgcgg gtaaccggca attgccgagg cagatgaata
     1081 tccaccggtg aaatggtctc cgttcaccca gtcagtaggc catgatggac caccagtcgg
     1141 cgcgaccagc gcatccagcc ggtgctgctt catgatgaag tcgatacctt gtgttcgtgt
     1201 cagcagatga tttttcctta acgctgcaag atatgccttg cttgtcaacg gacccttctc
     1261 ctgcgctttg atcattatgt cctgcccgaa atatggcatc tctttggcgc ggttgcgttc
     1321 gttaaaatcg atgacctctt tcaggttatg caccggagcc tgtgggccaa gtccagcgag
     1381 atacgtgttt aagtcagctt tgaactcgta aagtaaaacc tcaagctcgg aaccatcaaa
     1441 cttgccagta gttggaatat cggccggatc caccagcact gcgccctgct ttttcatctc
     1501 tgccagcaga tcgctcataa gtttgtcgac ctgatcgcta aatccgaagt gcttccgggc
     1561 gatgcctaag cgaacacctt tgagtccgtt aacgtcgaga aaagtcgtgt aatcggtacg
     1621 ggtaccacgc gaactattcc tcgtttccgg gtcgcgcgga tcaacccctg tcagagtccc
     1681 gagaattgca gcggcgtcag ccaccgtgcg agccattggt ccagctgtgt cctggctatg
     1741 cgaaatcggg atgattccgg ctcgactaac taaaccaacc gttggcttga tgccgaccaa
     1801 cgagtttgcg gatgaaggac aaaccacaga accgtctgtt tcagttccga tggccgcggt
     1861 acacaggttt gcagcgacag ctgcgccgga accagaactt gaaccacacg gattgcggtc
     1921 gagcacatac gggtttttag tttgtccgcc gcgcccgctc caaccactgg acgaatgatt
     1981 cgagcgaaag ttggcccatt cacttagatt tgttttacca agtatcacag cgccggccgc
     2041 tcgcagtttt tcagccacaa aggaatcacg tgtcggtttg gcgcctacca gcgcgagcga
     2101 gcctgcagta gtcatcatgc gatcggtagt gtcaatgttg tccttgatca ggatgggaac
     2161 tccgtgtaat gggccacgca ctcgtccagc ttttcgctcc tggtcaagcc gcgtcgcgat
     2221 cgactccgcc tcgggattca actcgatcac ggaattgatc gctggaccac gtttatcgac
     2281 gtcgttgatc cggtccgaat acttttcaac cagcgaacgc gaagaaaact ttcccgattg
     2341 cattccctgt tgcagttgct ggatggtcat ttcgtcgagc tcgaatgcac gaggctgtgg
     2401 gaaagttggt ttggatgtct cagacgttgt tgcaggggag acgggcgtta cgattgccaa
     2461 tgcgcaagtt gcagtggtgg cctttagaaa ctgtcgacgt ttcatctgat gggatccttg
     2521 ttttcaggag caggaaagaa cggttttgtg ctttgactac tcggcgattt tgaccttgaa
     2581 ggattcgaaa aagcgattca cgttgatgct gtcgttctct tttttgtagg tgccaactac
     2641 aaccagatac ggacgtcgac caaccatgta gattcgaccc ttgtattcgc gttttgcagt
     2701 ctcagcggta aactccaacg actgatagcc atctatagtt aagttcttac tggtcagaag
     2761 tgtgcccgaa atgccgttta cgaagttatc ccgattgaga attagctctg gctggacgaa
     2821 gtaaaagtta tagcttggat cgtattctcc ccaggctacg taaaaggcgc tttggggagt
     2881 gcttgcggtg aaggtatgtg tggaatacgg accgcgttcg ctttcaatcg tttcgctgcg
     2941 gctggatgga gcttcgggca tctttacgga aaagcggccc acatctgaag taaaagtgat
     3001 ccaggaatca cgctcaagtc tggttttgct atgtgagctg ttctgagcca acgttagtga
     3061 gccagtgaca gttgcgagta aaaaagccac caagatcctg ctcatacaaa gtccttcgac
     3121 ctggaactcg attccgaata aggcttatac cgatcccttg caaggtagtc aattggaggt
     3181 tgagttttgt gtgacattgg cattccaccc acttttgtcc taccttctat tctttttcag
     3241 gaatccaatg aattcaccag aaccaacgca agcacccaga ccgcgacgac tagtcgccat
     3301 tgtcggacgc cctaatgtcg gcaaatcaac gctctttaac cgattgattg gagagcgacg
     3361 ggctatcgtc ggcgacgaac cggga
//
LOCUS       DASSFP010000367         6118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_846454, whole genome shotgun sequence.
ACCESSION   DASSFP010000367 DASSFP010000000
VERSION     DASSFP010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846454"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2485)
                     /locus_tag="VFH91_08260"
     CDS             complement(71..2485)
                     /locus_tag="VFH91_08260"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6863027.1"
                     /translation="MTDTSVLSSKPSVYSQSTIYLIGLCFILSGATGLIYEVLWARML
                     GLVFGATTLAVSTVLAAFMGGLALGSAIAGRVGGRLRKPLSTYGWLELGIAVYALLVP
                     LIFALVDRLYVLIWQQFHPGFFAFSVWRFVLSCLLLLVPTTFMGATLPVLSAALLDSV
                     GHKRSSVATLYTCNLAGAILGTLLAGFVLLPTIGVTKTIAVAAALNAIVAVVAIIAQR
                     SYSTLTQSPIAVTKEIDSTPGTRIWLLAAFVSGFVTISSQVSWTRILTMVIGSSTYAF
                     SLVVALFLIGLAAGASIVSRFVKDSVLRNSLMKVEIVTAVSLVLSLYVVNLIPALVVS
                     LGLNLKIHSWIGLLSLQVLAASLLVLLPALLMGMVMPLVLVWASADEKNAVKLVGRSY
                     AINTVGAIVGAFAAGFVLIPKINTRFTILFAATTCLIVAGLAYEPDTTASDPAIRKSI
                     AIGAVPVLVLAMFFIFAPHMNKVDLSIGAYDSLIRILAKSREGGNDEISDQQKTDTHK
                     LLMYSEGPTATVSVREDQGTISLAINGRTNASDSSYDMPTQVMLSQIPLLVAPKINNA
                     LIIGFATGVSVGALLRSPIESVTCVELETATIEASRFFNHVNHLPLDDPRTHLIIDDA
                     RTFLRVTPNKYDMIISEPSHPWVPGVANLFTKEFFELGRERLSDQGVFVQWVQIYQLS
                     TESLKSVLATYHSVFPHVLMFRVGGAEKGKDLILIGSDQRLNLNHLSERFADPGMAAE
                     VGRVNLKSENDVRDWLVCDESRLGPAVQGSMINTDDNMFIETTVPREAFRPLMQSNSE
                     WVGSLK"
     gene            complement(2534..3910)
                     /locus_tag="VFH91_08265"
     CDS             complement(2534..3910)
                     /locus_tag="VFH91_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6863028.1"
                     /translation="MSEDLERSNIFARLIPQTSSIVRIVLGTLLVLAGLAAAVVTVLA
                     RRQNEPALAGAAAIMSLIIALLLTVFIVPPLARSARMEIANLDFPFEPTSGGGVFIII
                     IIVVGFAAWNTGNNLLFLVFSLLCSTLFVGWIAARASLRDLTVSARFPDHIFAGESAP
                     VIVTLRNTKRVLPSFSIVVEARGPNTEIQPNGERSLKKYANRLLAYFSYVPHHAAAEQ
                     RVEQLFPNRGHVLIDGFELSTRFPFGFFRRRRRLRARNVDIIVYPKPDVISDDLHLLP
                     MYTGRLASTRRGAGHDLFSMRDYQPQDDLRYIDWKATARSRKLTVREFTSEDERRITI
                     VMDTRLADESDEDLRHELRERFEGGVVQTASLVKHFIDERAEVRLVLGDEVGAFGSGR
                     EHLYRCLRRLALVLPAIEGRDVEVEAKLKRSMEPDTVEHDFAILLTAAPPGSIPASVW
                     RTSHVIYL"
     gene            complement(3938..4921)
                     /locus_tag="VFH91_08270"
     CDS             complement(3938..4921)
                     /locus_tag="VFH91_08270"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6863029.1"
                     /translation="MRNLEQIDSPLTSGRSAIEQIERLQQAIETVIKGKPEAVRLAII
                     TLIAGGHLLVEDVPGVGKTTLAHALARALDCNFQRIQFTSDLLPSDVIGLSVYNQHSG
                     SFEWKPGPVFANIILADEINRTTPKTQSALLEAMAERHVTVEGETHPLPSPFIVLATQ
                     NPIEHHGTYPLPESQLDRFMLRLRIGYPNAADEERILRDREYADPLDELEPVMRNHDV
                     LELQRAVTTVSVDDSLVQYLMRIVAATRDSEMLDLGVSPRGTLALFRAAQALALGEDR
                     TFCIPDDIKRLVVPVFAHRIVVSSRFSSSLRRSEEAEAVLNEILKTVSVPL"
     gene            5170..5658
                     /locus_tag="VFH91_08275"
     CDS             5170..5658
                     /locus_tag="VFH91_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6863030.1"
                     /translation="MKPQNRDKNASQRGFSLIELMIVIAIIGILIAVGVTGYKAAMKA
                     ANEAAAIKTLRSIAEQQMLYFNAHQRTTFGTFDEMRKENLLDTRFSGTTPVVDGYVYN
                     MKVIPKSTAQPAGYTINADPQVAEGVSATGKNHYFVDSDSNTIHVNDSQPATSTDPPI
                     GQ"
BASE COUNT         1639 a         1609 c         1482 g         1388 t
ORIGIN      
        1 cgcgatgatg ttaggcttaa cttaattggt ccagtccagg cacattctct tcctcatagg
       61 actactgctc tcacttcagc gaaccaaccc attcggaatt tgactgcatc aacggacgaa
      121 aggcctcgcg cggaaccgta gtctcgataa acatattgtc gtcagtattg atcatcgatc
      181 cctgtactgc cggtccaagc cgtgactcgt cacacaccaa ccagtctcgc acatcgttct
      241 cagacttgag atttacacga ccaacctccg cagccattcc tggatccgca aaacgttccg
      301 ataagtgatt cagattcagc cgctgatcgc tgccaattag tattaagtcc tttcctttct
      361 ctgcaccgcc aactcgaaac atcaggacgt gagggaaaac cgaatgatag gtagccagca
      421 cggatttcaa actctcggtc gacagttgat agatctggac ccactgcaca aagactccct
      481 ggtcactcaa gcgttctcgt ccaagctcaa agaattcttt cgtgaataga ttcgcaactc
      541 ccggaaccca tgggtggctc ggctcagaaa taatcatgtc gtatttgttc ggcgtgacac
      601 gaaggaaggt ccgtgcatca tcaataatca aatgagtgcg aggatcatcc agcggaagat
      661 gattcacgtg attaaaaaag cgcgaggcct cgattgtcgc ggtctctaac tctacgcacg
      721 tcaccgactc aattggcgaa cgcaacagcg ctcctacgct aacgcctgtc gcaaaaccta
      781 ttatgagagc gttgttgatt ttcggagcga ccagcagcgg tatctgcgaa agcatgactt
      841 gagtgggcat gtcataacta ctgtcggacg catttgttct tccgttgatc gcaagtgaaa
      901 tggtgccctg atcctcccgt actgagacag ttgcagttgg tccttcgcta tacatcagca
      961 gcttgtgagt gtcggtcttc tgttgatcag atatctcgtc attcccacct tcccggctct
     1021 tggcgagaat gcgaatcaag ctatcgtaag caccaatcga aagatcaacc ttattcatgt
     1081 gcggcgcaaa tatgaaaaac atggctaata caagaacggg tacagctccg atggcaatac
     1141 ttttcctgat agcaggatca ctggctgtag tgtctggttc atatgcgaga cccgccacta
     1201 ttaaacaagt tgttgcggca aataagatcg taaatcttgt attgatctta ggaatcaaca
     1261 cgaagcccgc cgcgaaagca cctactatcg ctccaaccgt attgattgcg tagctacgcc
     1321 cgactaactt gaccgcgttc ttctcgtctg cactggccca cacaagcacc agcggcatta
     1381 ccattcccat cagcaacgcc ggtaacagca caagcaaact agcggctaac acctgcaaag
     1441 aaagcaaacc tatccatgaa tgtattttga gattcagccc gaggcttacc accaacgccg
     1501 ggatcaaatt aacaacatac aaactaagaa ccagagacac ggccgtaacg atttcgacct
     1561 tcattaaact atttcgcagg actgagtcct taacaaatcg cgagacgatt gatgctcccg
     1621 ccgcgaggcc gatcaagaag agcgcgacaa ccaagctgaa cgcatacgta cttgagccta
     1681 tgaccatcgt taaaatccgt gtccacgaca cctgactact gatagtcacg aagcccgaca
     1741 cgaacgcagc caacaaccag attctcgttc ccggtgtgga gtcaatttct tttgtaacag
     1801 ctatcggcga ttgcgtgagt gtcgaataac tacgttgcgc gatgattgct acgacagcaa
     1861 ctattgcgtt aagggctgct gcaactgcga ttgttttagt cacgccaatc gttggcaaga
     1921 gtacgaagcc tgcgagcagt gttccgagta ttgctccggc cagattgcag gtgtagagcg
     1981 tcgcaactga actccgctta tggcccaccg agtcaagtag cgccgccgac aagacaggca
     2041 acgtggcacc catgaaagtc gtcggcacta aaagcaacaa acacgatagg acaaagcgcc
     2101 agacgctaaa cgcgaaaaac ccgggatgga attgctgcca gattaagaca tataacctgt
     2161 cgacaagtgc aaagatgagg ggtactaaca gcgcgtaaac tgcaattccc aactccaacc
     2221 aaccatacgt gctaagaggt ttgcgaaggc gtcctccaac acggccggcg atagcgctac
     2281 ccagcgccaa tccacccatg aaggccgcca gaacagtgct gactgccaat gttgtagctc
     2341 cgaatacgag gcccagcatg cgggcccaaa gcacttcgta gatcaatcct gtggcgccgg
     2401 acagaatgaa gcagagccca ataagataga ttgtcgattg tgaataaacc gagggctttg
     2461 acgagagcac tgaagtgtcg gtcaagacta atcgttcctt atcgttcgcc ggaatttaag
     2521 actctgagac aactcacagg taaataacat gtgaggtgcg ccagacactt gctggaatgg
     2581 aacctggagg cgccgccgtc aggaggatcg caaaatcgtg ctcgactgta tcaggttcca
     2641 tgcttcgctt cagctttgcc tccacttcga catctctacc ttcaatcgca ggaaggacca
     2701 gagcgagacg acgtaagcac cgatatagat gttctcgacc cgagccgaac gcgcccactt
     2761 catctccaag aaccagtctc acttcagctc gttcatcgat aaagtgcttt acgagggacg
     2821 cagtctggac cactcctcct tcaaacctct cgcgcaactc atgtctcaag tcttcatccg
     2881 actcatctgc gaggcgagta tccattacta tggtgatgcg ccgttcgtcc tctgaagtga
     2941 attcgcggac agttagcttg cgcgaacgtg cagttgcttt ccagtctata tatcgcagat
     3001 cgtcctgtgg ctgataatct cgcatcgaga agagatcatg tccggcaccg cgtcgggtag
     3061 aagcgagtcg tccggtgtac attggaagaa gatgcagatc gtcgctgata acgtcgggtt
     3121 tcggatagac aatgatatcc acatttcgag cacgcaggcg acggcggcga cgaaagaatc
     3181 caaagggaaa gcgagttgaa agttcaaagc catcgatcag tacatgaccc cggttcggaa
     3241 agagctgctc aacccgttgc tcggcggccg catggtgtgg gacataactg aagtaagcga
     3301 gtagccgatt ggcatacttc ttcaaagacc tctctccatt gggttgaatc tcggtgtttg
     3361 gaccacgagc ttcgaccaca atcgaaaatg atggaagaac ccgctttgta tttctcaacg
     3421 ttacaataac gggcgcagac tcacctgcaa atatgtgatc cggaaatctc gccgacacag
     3481 tcaaatctcg taaagaggct cgtgcagcaa tccacccgac aaataaagtt gagcaaagca
     3541 atgagaagac caaaaacaac aggttgtttc ctgtattcca tgcagcgaat cccaccacaa
     3601 tgattatgat gatgaaaacg ccgcccccac tggttggttc gaaaggaaaa tcgagattgg
     3661 cgatctccat tcgtgctgag cgcgcgagtg gtggaacgat gaaaacggtg agcaggaggg
     3721 caatgatgag cgacattatt gccgctgctc ccgccagggc cggctcgttt tgtcgtcgag
     3781 cgagtactgt tacgaccgcc gccgctaaac cggcaaggac gagaagagtt ccgagaacta
     3841 ttcgtacgat tgatgaagtt tgagggatta agcgggcgaa aatgtttgag cgttcgagat
     3901 cttcactcat tcctgatcgc ttcgtccttc acgactttca gaggggaaca ctgacagttt
     3961 tgagaatttc attcagcacg gcctcggctt cttcgctacg tctaagagaa gaagaaaacc
     4021 gcgaactaac aacaattcga tgagcgaaaa caggcacaac caaccgctta atgtcgtcag
     4081 gaatgcagaa ggtgcgatct tcacctaagg ccaatgcctg ggcagcgcga aataacgcga
     4141 gtgttccgcg agggctaact ccaaggtcca acatttctga atctcgcgtc gcggcaacaa
     4201 ttctcatcag atactgaacc aatgaatcgt ccactgacac tgttgtcacc gcacgctgga
     4261 gctccagtac atcatgattt ctcatcaccg gctcgagttc atcgagggga tcagcgtact
     4321 ctcgatcgcg tagtatgcgt tcttcgtcag ccgcatttgg atatccaatc cgtaatctca
     4381 gcatgaaccg atccagctgc gactcgggaa gtggataagt gccatgatgc tcaatcggat
     4441 tctgagtggc gagcacgata aacggcgacg gcaatggatg cgtttcacct tccactgtaa
     4501 cgtgtcgttc agccatcgct tcgagcaaag ctgactgtgt tttgggggtt gtccgattga
     4561 tttcatccgc caggattatg ttggcgaaga ccggaccagg tttccactcg aaggatccgc
     4621 tgtgctggtt atagactgac aggccaataa catccgaagg tagcaggtca gatgtaaact
     4681 gaatccgttg aaaattgcag tctagtgccc gggccaaagc gtgggccagc gtcgtttttc
     4741 cgacccccgg tacatcctcg actaggagat gtccgcccgc aatcagggta atgattgcca
     4801 ggcgaacggc ttcaggcttt cccttgatga ccgtctcgat tgcctgctga aggcgttcaa
     4861 tttgctcaat agcacttcgg ccagaggtca gaggagaatc gatttgctct agatttcgca
     4921 tgaattcgag gtcattctac atgctaagag catcagcggc atcaaaaagt tggctacacc
     4981 accggatact ttgcgttact gttccattcg cactcaattc atgcacgact tatagaatta
     5041 actaactgga ctgactctgt gacctggaag ccgtcatgta gtagacttag ccgtaataat
     5101 ttacgggcac ggtagacaga aatcggaacc tgaccctctg cggatcgtta aagtgctcga
     5161 ggaggcatta tgaaacccca aaatagagat aagaacgcga gccagcgagg tttttcgctc
     5221 attgagttga tgatcgtgat cgcgatcata ggaattctca tcgcagtggg cgttacgggc
     5281 tataaggcgg ccatgaaggc ggcaaatgag gcggccgcca tcaaaacgtt gcggtccatc
     5341 gccgaacagc agatgcttta ctttaacgct catcaacgaa cgacatttgg cacttttgat
     5401 gagatgagaa aagaaaatct gctcgataca agattttcgg gaacgacgcc agttgtcgac
     5461 ggttatgtct acaacatgaa ggttattccc aaatccacgg cccaacctgc cggctataca
     5521 atcaacgcgg atccccaggt tgctgaaggc gttagcgcca ctgggaagaa ccattatttt
     5581 gttgactcag attccaatac gattcacgtt aacgatagtc aaccagccac ttcaaccgat
     5641 ccaccgatcg gccagtaacc acgagagcta cttttaagga caggacggtt gaggcgcaga
     5701 tcacacgcct caaccgtttt ttattgcctc gacctgacaa cccagaaccg caagctccta
     5761 acttggctta ctctgtgcaa cctttgttgc tctgtgcctt ctgtggtgag caaagtccca
     5821 aggaaaacca ccaccacaga gaccgacgag tctcagaggc agcacagaga aggctcagat
     5881 taggacaccg ctccttgttc tgtaacctgg ccaaactgaa gacctcgaca attctgttcg
     5941 gcacaaaatc gtagaacgat tgccacgcac tttcgagaat tgtctgctta agatacggaa
     6001 taaaacgatc gttgggccga ctgatgcgat gcactttcgc caggaccgtt gggccaacga
     6061 gtgcattttg gaggctcatc aatgccaatt cgcagatcac tcatttccgg gatgttgg
//
LOCUS       DASSFP010000368         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_546544, whole genome shotgun sequence.
ACCESSION   DASSFP010000368 DASSFP010000000
VERSION     DASSFP010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546544"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="thiE"
                     /locus_tag="VFH91_08280"
     CDS             complement(<1..642)
                     /gene="thiE"
                     /locus_tag="VFH91_08280"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6863031.1"
                     /translation="MSPKLQPPITYLITSGKTTAITTPESPDFSQLLQLIEAAVLAGV
                     SLIQIREKNLPVRVLADLCARASTVTRGSETKLLINDRVDVALAYGADGVHLTSQSLS
                     ADVARQVSGADFIIGVSTHSFNEVIEARNGGADFAVFGPVFDTPSKLHYGEPQGLDKL
                     REISSSLGELRVLAIGGVSLENVEECFAVGAAGVAAIRLFEDAATLSHRIKAIN"
     gene            complement(569..988)
                     /locus_tag="VFH91_08285"
     CDS             complement(569..988)
                     /locus_tag="VFH91_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863032.1"
                     /translation="MENSGVQFRSSLSARLSVVFYIILCFEIGIFLTVLPWWPQGMWG
                     LSDWGNNYFLLYAARKTGQGLQTVVASGWVRGAVTGVGLLNLGIAFWEIFHFNQTVRA
                     LDGQSGPRVKPAGNVTQAAATDHLPDNERQNDSDNNS"
     gene            complement(989..3289)
                     /locus_tag="VFH91_08290"
     CDS             complement(989..3289)
                     /locus_tag="VFH91_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6863033.1"
                     /translation="MAFEKAKVLKAAEKFLSQGKINAAIKEYRQIVDNDVDDLTTLNM
                     LGDLYVRASKKEEAIKCFDRIAEHYRTQEFNLKAIAMYKKIDRLRPRDPSVANKLADL
                     YASQGLIVDARAQYLIVADAYTRGGDNRRALDILHRIADLDPNNVDIRLKLAEGYVKE
                     SMRTEAALAFVQAANRLQQVASIDRSLEAYSKALQLIPDDVDALRGLLESHIARGTPD
                     EAAEVLEKVVETRDSDKELVSMLARAHLEAEDPAGAERAVMLLMKQDSSNYPQILPVA
                     RLYLKNDEVDETLRVLEGIIERMLAGREERELLELVNSVLERDPDRVPALRMLVRIYW
                     WQRDMDSLRSALERLAEAAEAAELVDEERYALTQLVRLAPDEPRYLDRLNLIGGLQEE
                     TVEDFTAQSEPEPEPPRSDDVPQFETFAVVDTDEDVATPVEVVQFETNAGPAQAVSDP
                     TSSFADLNDDQAFASEVDSKAERFQEVSFADLSVAADEETEPSDVVPDRARQENMMRQ
                     ELESVDFYIAQGYADIAIDTLEMLERQFGSHPEIEVRRAKLAEGQKAAGEAPAVFEFG
                     GAEESNNAQAKDPGLAFSFTSHTAESNTAASPTSASASAPAPAAKGIDSGLAELFEEF
                     RTAEEDDLGHEDYETHYNMGTAYKEMDLVDEAIQEFQTSVALVKPGDGTSRFLQCCNM
                     LGHCFLEKGMPEAAIMWFKKGLAAPGHTEDEYQALRYELALAYEESGDLTNAQQLYTE
                     VYGVDVSYREVGDKLSLLKKKMQESR"
BASE COUNT          829 a          944 c          829 g          861 t
ORIGIN      
        1 attgatagcc ttaatacgat gcgagagtgt cgcagcatct tcaaacaatc tgatcgccgc
       61 tactccggca gcacccaccg cgaaacactc ttcaacattc tcgagactta ccccgccgat
      121 ggccaacacc cgcaactcac ccagagacga agaaatctct cgtagcttat cgagaccttg
      181 cggctcgcca taatgtagct tcgatggagt gtcgaacact ggtccaaaaa cagcgaagtc
      241 cgctcctcca tttctggcct caatcacctc gttgaaggag tgagtcgaaa cgccaatgat
      301 gaagtctgcg ccagatactt gacgtgccac atccgcactc aaactctgac tcgttaagtg
      361 aactccatcg gctccgtatg cgagcgccac atcgacacgg tcgtttatca gaagttttgt
      421 ttcacttcca cgcgtaacgg tggacgcacg agcacataag tctgcaagca ctcgtacagg
      481 cagattcttt tctcggattt ggattagcga gacgccggca agtacggctg cttcgattag
      541 ttgaagcagt tgggagaagt cggggctttc aggagttgtt atcgctgtcg ttttgccgct
      601 cgttatcagg taagtgatcg gtggctgcag cttgggtgac atttccggct ggttttactc
      661 gtggaccaga ctgaccatcg agggcacgaa cagtttgatt gaaatggaaa atttcccaga
      721 aggcgattcc gagattcaat aagccaactc cagtgactgc accgcggacc catcccgacg
      781 cgactaccgt ctgaagtccc tgtccggtct ttcgcgcagc atataacagg aagtagttat
      841 ttccccaatc actcaggccc cacattcctt gtggccacca gggcaagacg gtaagaaaaa
      901 ttccgatctc gaagcagagg ataatataga agacaacact gagtcgtgcc gataaagagg
      961 atctgaattg gacgccactg ttttccaact atcgactttc ctgcatcttc tttttcagga
     1021 gactaagctt gtcgcccact tcacgatagg agacgtcaac tccgtaaact tctgtgtaaa
     1081 gctgttgcgc gttggtcaaa tcaccagact cttcatacgc taaagccaat tcgtaacgca
     1141 acgcctggta ttcgtcttca gtgtgaccgg gagcagccaa tccttttttg aaccacatga
     1201 tcgcagcttc aggcattccc ttctcgagga aacaatgacc gagcatatta caacactgaa
     1261 gaaaacgaga tgtaccgtcg cccggtttta ccaaagcgac tgaagtttga aactcctgaa
     1321 tcgcctcatc gaccaggtcc atctctttat aagcagtacc catgttgtag tgggtttcgt
     1381 aatcctcgtg acctaaatcg tcttcctcag cggtgcgaaa ttcttcgaat aattctgcaa
     1441 gacccgagtc aatgcctttg gctgccggag caggcgctga cgccgacgct gaggttggac
     1501 tcgctgccgt attagattcc gcagtgtgac tggtaaaaga gaaggctaag ccaggatcct
     1561 tagcttgagc gttgttggat tcctccgcac ccccaaactc gaatacagct ggagcctcac
     1621 ctgcagcctt ttgtccttcg gcgagttttg ctcgtcgaac ctcaatctct ggatgagatc
     1681 caaactgtct ctcgagcatt tcaagagtat cgatagcgat gtctgcatag ccctgagcga
     1741 tgtaaaaatc gacactctct aactcttgac gcatcatgtt ttcctgacgc gctcgatctg
     1801 gaactacgtc agagggctcg gtctcttcat cagcagcaac cgacaaatca gcaaaactga
     1861 cctcttgaaa cctttcggct ttcgagtcca cctcagacgc gaaggcctgg tcgtcattca
     1921 aatctgcaaa cgacgaagtg ggatcagaaa cagcttgtgc ggggcccgca ttggtttcaa
     1981 actgaacgac ctccaccgga gttgcaacat cctcatccgt gtctaccacg gcaaacgttt
     2041 caaactgggg aacatcgtca gatcttggag gttctggctc tggttcgctc tgcgccgtga
     2101 aatcttcgac tgtttcttcc tggagaccgc caattaagtt cagtctgtcg aggtatcgcg
     2161 gttcatctgg cgccaggcgg actaactgag tcagggcata acgttcttca tcgacaagtt
     2221 ccgctgcttc agcggcctct gctaatcgct ccaaagcaga acgcagcgaa tccatgtcac
     2281 gttgccacca gtagatccgc acgagcatcc gtagagcagg aacgcgatct ggatctcgct
     2341 caagcacgct attaacgagt tcgagcagtt ccctttcttc gcgtcccgcc aacattcgct
     2401 cgatgatccc ttccaaaacg cgaagggttt catcaacctc gtcattctta agataaagtc
     2461 tcgcgaccgg aagaatttga ggatagttcg acgagtcctg cttcatgaga agcatcaccg
     2521 cgcgctccgc ccctgctggg tcttcagcct caagatgggc gcgcgctaac atcgaaacga
     2581 gttccttatc cgagtccctg gtctcaacta ctttttcgag tacttcagct gcctcgtctg
     2641 gagttcccct ggcgatgtga gattcgagca gaccccgcaa agcatccaca tcgtcgggaa
     2701 tcagttgtag cgctttggag tacgcctcaa gcgatctatc gattgacgca acctgctgaa
     2761 ggcggttcgc ggcttggaca aaagccaacg cagcttcggt ccgcatactt tctttcacgt
     2821 agccttcagc cagttttaag cggatgtcca cgttgttggg atcgaggtca gcaattcggt
     2881 gcaggatgtc gagggctctt cgattgtcac cgccgcgggt ataagcgtcg gcgacgatta
     2941 aatactgggc ccgcgcatca acaatcaatc cctgacttgc gtaaagatcc gcaagtttat
     3001 tagccacaga cggatctcgc ggcctgagcc ggtcgatctt tttgtacatc gcgatggctt
     3061 ttagattgaa ttcctgagtg cggtagtgtt cggcaatccg atcgaagcat ttaatcgctt
     3121 cttccttctt actcgcacgg acatacaaat caccgagcat gttcaaggtg gtaaggtcat
     3181 ctacgtcgtt atcgacgatc tgacgatact ccttaattgc tgcgttgatc ttcccttgcg
     3241 ataagaattt ctcggcggcc tttaggacct tagctttttc gaaagccatc agggatatta
     3301 tgtatcaata atgtggtcgg tggcgttaat cgtagcggcg ggcagcggga caaagcaatc
     3361 aagttaagcg cttgtcaata ctccactttc cttaccccgg gaaacagccg tcagatacgc
     3421 taacatgcga aataaagggt gtcaacgcaa tggtttgtcg tcc
//
LOCUS       DASSFP010000369         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1137142, whole genome shotgun sequence.
ACCESSION   DASSFP010000369 DASSFP010000000
VERSION     DASSFP010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137142"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFH91_08295"
     CDS             complement(<1..786)
                     /locus_tag="VFH91_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6863034.1"
                     /translation="MSFIDILFLALRNLRQAKLRAILTTMGVIVGVAVIVTMVSFGLG
                     LQHNMLARFKALDLFNEIQVFGQGLSNLAGLDRPTRRDEGERRNARQRADKTPTRILD
                     DAGVKEISAIPGVSYVEPNVNFGAYVRSNGKLLTQSVGGANIPNASTRFQKFTAGHMI
                     SSPSADEAVVTERFTRDYGYATPAEAIGKTLELLAPPDETNDQNKKEEAESTPNFFGI
                     PLDDPGLDESDSSLETKTFRISGVLNTEIKEGAGQGGLRGLMPG"
     gene            complement(783..1487)
                     /locus_tag="VFH91_08300"
     CDS             complement(783..1487)
                     /locus_tag="VFH91_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET6863035.1"
                     /translation="MLQAIFFDFNGVIIDDESIQMKAYQEVLREHQIDLTEEWYFGAL
                     GMDDKTFVRAMFERAKQPLSEEDFNKVLSAKTELHRKLIEDELPLFRGILTFLKSTAR
                     HFSLGLVSMANPVEIGYVFERAHLNSLFSVVITSEDVTRCKPAPDCYTEGLAKMNAYR
                     QNKRLLPLLAEECLAIEDSPPGIQAAAKAGMKTLGITNTVKESELREAGADVVTHSLA
                     DWTVDSVKLVFDRHYA"
     gene            complement(1491..2372)
                     /locus_tag="VFH91_08305"
     CDS             complement(1491..2372)
                     /locus_tag="VFH91_08305"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET6863036.1"
                     /translation="MPNPATQMTLESLRSVPLFASLDDSAAIHLRNLLSEQEVPSNTR
                     LFNQGDQGNAMYLIESGRVRISIRDEDKQELTLAELAQGDFFGEMSLIDGRQRSADAN
                     VIEDSRLAILSRDSFLIFVRSNPDVALEMLSALSDRLRRTDQLLRSRVSKNANEEEKA
                     RLTLSDRAADMIAEFGGSWKFIGVSIGLIIFWIIFNSYILVRGFDPVPYQMLNLVLAV
                     IAGMQAPIIMMSQNRQGEKDRLRADLDYQVNLKNELSLAEVLRRLDVIESERLPKLFE
                     EQNSRLSNLKVNIDVED"
BASE COUNT          628 a          652 c          580 g          634 t
ORIGIN      
        1 gccaggcatc aatccacgta atcctccctg ccctgcacct tctttgatct ccgtgttcaa
       61 aacacctgag attcgaaacg tctttgtttc gagacttgaa tccgattcat ccaaccccgg
      121 atcatccaaa gggattccaa aaaagttggg agtcgattcc gcttcttctt ttttattttg
      181 atcgtttgtt tcatcgggcg gcgccagcag ctcaagagtc ttgccaatgg cctcggccgg
      241 cgttgcgtag ccatagtccc gggtgaacct ctccgttacc accgcctcat cagcagacgg
      301 agacgagatc atgtgtccgg ccgtaaactt ttgaaacctt gtcgatgcat tcggaatgtt
      361 ggcgccgcca acggattgag tcaggagttt tccgtttgaa cggacgtaag cgccgaagtt
      421 tacatttggt tcaacgtagg agactccggg aattgcagaa atttctttga cacccgcgtc
      481 gtccagaatt cgcgtgggag ttttatcggc acgttgtcgt gcattgcgtc gttctccctc
      541 atcacgacgc gtgggtcgat cgagtccagc gagattgctc aagccctggc caaagacctg
      601 gatttcgttg aagaggtcga gggctttgaa acgggcgagc atattgtgct gcaagcccag
      661 accaaacgag accatggtga cgatgacagc aacccccaca atgaccccca tcgtggtcaa
      721 gattgcgcgc agtttggcct gtcgcaaatt tcggagcgcg agaaaaagga tgtcgataaa
      781 actcatgcgt agtgcctatc aaaaacgagc ttgaccgaat caacggtcca atcagccaga
      841 ctatgtgtta cgacatccgc accggcctca cgcagttctg attctttgac cgtattcgtt
      901 atgccaaggg ttttcatccc agctttggca gcggcttgaa tacccggcgg cgaatcttcg
      961 attgccaaac actcttcagc cagaagcggt aagagtcgtt tgttctgcct atatgcattc
     1021 atctttgcca gtccttctgt gtaacagtca ggcgccggct tacaacgagt gacgtcctct
     1081 gatgttatta caactgagaa gagcgaattg agatgggctc gttcgaagac atatccaatt
     1141 tcaaccggat tcgccatgct taccagaccc agggaaaaat gtctggctgt actctttaag
     1201 aaggtaagga ttccccgaaa aagtggcagc tcatcttcga tcagctttcg atgcaactct
     1261 gttttcgcac ttaagacttt attgaaatct tcttctgaca gcggttgctt tgcacgctcg
     1321 aacatcgcac gcacgaacgt tttgtcgtcc atgccgagcg ccccgaaata ccattcctcg
     1381 gtaagatcga tctggtgctc acgcaaaact tcctgatagg ctttcatttg aatcgactcg
     1441 tcgtcgatga tcactccatt aaagtcgaaa aagatggcct gcaacataat ttagtcttca
     1501 acgtcgatat taactttcag gtttgataac ctcgagtttt gctcctcgaa taacttcggc
     1561 aaacgctcac tctcaatcac atccaaccgg cgcaacactt cggcgagtga caattcgttt
     1621 tttaagttaa cctgataatc gaggtccgcg cgaagtcgat ccttttcgcc ctggcgattc
     1681 tgcgacatca tgatgatcgg agcttgcatg cctgcaataa ctgccagtac cagattcaac
     1741 atttgataag gaaccggatc gaagcctctc accagaatgt aggagttaaa aatgatccaa
     1801 aagataatta gcccgattga gactccgata aatttccagc tgccgccgaa ctctgcgatc
     1861 atatcggcag ctctgtcgga aagggtcaag cgagcctttt cttcttcatt ggcgtttttc
     1921 gaaacacgag aacgaagaag ttgatccgtc cggcgcaaac ggtcggacag tgcactcagc
     1981 atttccaacg ccacatccgg attgcttcgc acaaagatta agaaagagtc tcgggacaaa
     2041 atggccagtc tcgagtcctc aatcacattc gcgtcagctg aacgttgccg gccatcgatg
     2101 agcgacatct cgccgaaaaa atctccctgg gcaagctccg ccaacgtcaa ttcctgctta
     2161 tcttcatctc gaatactgat ccgcacacga ccactttcga tcagatacat tgcattgccc
     2221 tgatcgccct gattaaagag gcgtgtattg ctcgggactt cctgctccga cagaagattt
     2281 ctcaaatgta ttgccgcaga gtcatcgaga gaagcaaaga gcggtactga gcgcaaagat
     2341 tcgagcgtca tttgagtggc ggggttaggc atatttcctc ccaaaaagga atcacacagc
     2401 gatcaacaag tagaaaggtc cagccgcttg gttgatacgt tggtcagtac cgattcgcgg
     2461 tacgaacaaa gctttgaatg attatcattt gata
//
LOCUS       DASSFP010000370         5039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2194031, whole genome shotgun sequence.
ACCESSION   DASSFP010000370 DASSFP010000000
VERSION     DASSFP010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194031"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1294)
                     /locus_tag="VFH91_08310"
     CDS             complement(179..1294)
                     /locus_tag="VFH91_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HET6863037.1"
                     /translation="MKPSNRLISLDVFRGITIAAMTLVNNPGTWSSIYWPLEHAEWNG
                     WTPTDLVFPFFLFIVGVSITLAFGRRIEERGVTRDLYLKVIKRSLIIFGLGLFLNGFP
                     HFHLATIRIPGVLPRIAVCYLVASLIFLGTRVRTQFIIAIALLIFYYILMTKVAAPGF
                     AAGDLTKEGSLASYVDRVVFGPHIWKQGKVYDPEGILSTIPAVATTLFGILAGHWLRT
                     ERTHYEKVAGMFVAGAFCVMIGWACNPFFPINKALWTSSYVFFTAGLALQLLALCYWL
                     IDIKGYRRWAWPFEVFGMNAIALFVGSAVMVDLMGLIKIPAADGSQDSLQGWIFDHLF
                     LSWASPINASLAYAIAFVLFWLFLMWLLYRKNVFIKI"
     gene            complement(1402..3504)
                     /locus_tag="VFH91_08315"
     CDS             complement(1402..3504)
                     /locus_tag="VFH91_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HET6863038.1"
                     /translation="MANTLTRSLGSRLIVVSLFLLFVNGLVRAQTSDDSSAIAVIPKP
                     RQVTANAEAFALDRGTYLRLADSKSSEDKFAATDFIDDVKQTSGIVLRVSNGKRSRAI
                     LIGKLDQPVIQSALRSRSVSVNPSLGEEGYVLDVRKDGVIVAGTTASGVFYGLQTLKQ
                     LVRGDASNAYIQGVQIVDWPAMRWRGVSDDISRGPVPTLAYIKRQLRTFAAFKLNMHS
                     FYMEHAFASTEHPLIGAEGGSLTPAEIKELVEYARSYHIELIPEQQTFGHLHKALKLE
                     KYSSLAETPYGDVLSPQQEGSYKLIADWYRELDQLFPGQFFHIGADETFELGEGQSKE
                     VARAKGVGAVYFEHLDRVRDLLKPYNRKLMFWGDIALNHPDLIGDIPKDLIVANWDYS
                     PRDSYNSRIEPFKTAGLQQLVCPGVWSWNQIFPNVESSSKNIINFVRDGQTAGAIGML
                     NTSWDDDGESLFEMTWYGVVLGAAASWQSQPFDQKAFDAAFDWAFFRTEGQTFVGTIR
                     ALGSVSATLGGGAGDEAFWRNPFSTAFATQARANAEKIRQMRLQVENAEETLIRNRSK
                     VRRNGSMIPVMIFAAQRFDHLGRRAQTVEKFSREYWDAYLNLGDRTKARRLRRYTGAI
                     YNNLREMAEELAELKTSYREQWLAENRPYWMESILSRYDQAISIWLAKSRAMDDALRM
                     YDTTSILPNPEEFGLGTR"
     gene            3756..4781
                     /locus_tag="VFH91_08320"
     CDS             3756..4781
                     /locus_tag="VFH91_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET6863039.1"
                     /translation="MLSEITEQPKALAKTISEERNKLASLGKLLKSREIDLIVLVARG
                     SSDNASLFGRYLLEITTGIPVSLSAPSVHTIYNAKLKLDHALVVGVSQSGEGEDINRV
                     LENARSSGAFTVGITNEPNSSMTKLVDETLLTHGGRERSVAATKTFTGQMLLFYMLAS
                     ELASGKLSFNYEVIPDFVERALEQKPAILELVQRYVFMENCVIVGRGLAYANAYELAL
                     KLMETCYVVAERFSSADFLHGPLAMVERHFPVILFAPPGVMLPGVKSLIERLRELHAD
                     TLAITSDLDAAALCSRSIVMPKEIDEFVAPIPYIVPGQLFAALLAEAKGLNPDSPRSL
                     LKVTRTL"
BASE COUNT         1323 a         1306 c         1202 g         1208 t
ORIGIN      
        1 cggctgggca agctcgacct cgttccgagc cctcgacatg tccagaccct tgatctacgc
       61 cgaacctctc gaccttcttt caccatcaaa cgagaaactc gatccatggc ccaaatggga
      121 aatgaaaaat cgaaaatgat atatgaaaaa tgatcttctt ggctcttgtc ccacccttct
      181 agatcttaat aaacacattc ttcctgtaga ggagccacat caggaatagc caaaacagaa
      241 caaacgcaat tgcataagct agcgatgcat taatcggact tgcccacgat aagaagaggt
      301 gatcaaaaat ccatccctgt aacgaatctt gggagccatc agcagccgga atcttaatca
      361 gccccatcaa atcaaccatc acggcacttc cgacaaacaa tgcaatcgca ttcataccaa
      421 agacttcaaa gggccaggcc cagcgtcgat agcccttgat atcgattagc caataacaga
      481 gggccagtag ttgaagcgca agtcctgcgg taaagaagac ataagaactg gtccagagcg
      541 ctttgttgat tggaaagaaa ggattacagg cccaaccaat catcacgcaa aacgcacctg
      601 cgacaaacat gcccgcaact ttctcgtaat gcgttcgctc agtgcgtaac cagtggcccg
      661 ccaggattcc gaacagcgtt gtggcgaccg ccggtattgt gctcaggatt ccttcaggat
      721 cgtaaacttt gccctgtttc cagatgtgtg gaccaaacac gacgcgatca acgtacgacg
      781 cgagacttcc ctctttcgtc aaatcgccag ccgcaaatcc gggagcggca accttagtca
      841 tcagaatgta ataaaagatc agcaaagcta ttgcgatgat gaactgagtt ctaactcgcg
      901 taccgaggaa aatgagtgat gccaccaaat agcaaacggc aattcgcggc agcacgcctg
      961 gaattctgat cgtggcgaga tgaaaatgag gaaagccatt taaaaacagg cccagaccaa
     1021 aaatgatcag cgaccgtttg atgaccttta aatacaggtc cctcgtgaca cctcgctcct
     1081 caatgcgacg cccgaatgcc agcgtaatgg aaactccgac aatgaagagg aagaacggaa
     1141 agaccagatc ggttggagtc cagccgttcc actcggcgtg ttcgagcggc cagtagatac
     1201 tgctccacgt tccaggattg ttcaccagag tcatagcggc aatggtgatg ccccggaaca
     1261 catcaagtga gatcagtcgg tttgatggtt tcatttttgg agtgtggcgg ccgggccgct
     1321 ttctagtgcg gcgttacgcc gctgtttaat gagcgagttg acaggagtga caaagcggcg
     1381 ccgggccgcc gtactccaaa gttatctcgt gccgaggcca aactcttccg ggttgggaag
     1441 aattgacgtg gtgtcataca tgcgcaaagc gtcatccatc gcgcgactct ttgctaacca
     1501 gatagagatg gcctgatcgt aacgtgaaag aatgctttcc atccagtacg gtcgattctc
     1561 agctaaccat tgttctcgat agctggtctt taactccgcc agctcttctg ccatctctcg
     1621 gaggttattg taaatcgccc cagtgtaacg ccgcagcctt cgagccttag tgcgatctcc
     1681 gagattgagg tatgcatccc aatactcgcg gctgaacttt tcgacagttt gcgctcgccg
     1741 gcccaaatgg tcaaagcgct gggctgcaaa gatcattacc ggaatcatcg aaccgtttcg
     1801 tcgcaccttc gacctgtttc gtattaatgt ctcttccgca ttctcgacct gcagtcgcat
     1861 ctgcctgatc ttctctgcat tggcgcgcgc ctgagttgca aaagctgtac taaatggatt
     1921 tcgccagaag gcttcgtctc cagcgccacc tccaagagtc gcgcttacac tacccagcgc
     1981 tctaatggtc cctacaaaag tttgtccctc agttcgaaag aatgcccagt cgaaagctgc
     2041 atcaaacgct ttttggtcga acggctgtga ctgccacgaa gcagccgcgc ctaatacaac
     2101 tccgtaccat gtcatttcaa acaacgactc gccgtcgtca tcccagctcg tattcaacat
     2161 cccaatagcg cccgctgttt gaccatctct cacaaagttg atgatgttct tcgacgacga
     2221 ctcgacgttg ggaaatattt gattccaact ccaaaccccg ggacacacaa gctgttgtaa
     2281 acctgctgtc ttaaaaggtt cgattctcga gttgtaacta tcacgaggtg aatagtccca
     2341 gttcgcaacg attaagtctt taggaatatc gccgatgagg tcgggatgat tcaaagcaat
     2401 atcaccccag aacatcagct tacgattata gggtttgagt aaatcgcgta cccggtcgag
     2461 atgttcaaaa tagactgcac cgacaccctt agctcgagcg acctctttac tctgaccttc
     2521 gccgagttcg aatgtttcat cggcaccgat atgaaaaaat tggcccggaa acaattggtc
     2581 caactctcga taccagtctg ctatcagctt gtaactgccc tcctgctgcg gcgacagtac
     2641 atcaccataa ggagtttcag cgagcgaact gtatttttcg agcttgaggg ccttgtgtaa
     2701 gtgtccaaac gtctgctgtt caggaatcaa ttcaatgtga taagacctgg cgtattcaac
     2761 cagttcttta atctctgccg gtgtaagaga gcccccttct gctccaatga gaggatgttc
     2821 agtactcgca aaagcatgct ccatatagaa cgaatgcata ttcaatttga acgctgcaaa
     2881 tgtacggagc tgacgtttga tgtaggcaag agtgggaact ggaccacggc tgatgtcgtc
     2941 agacactcca cgccaacgca tcgcgggcca atcaacgatc tggacacctt gaatataagc
     3001 attcgaagcg tcacctcgaa ccagttgttt cagagtttgc aatccataaa aaactccgga
     3061 ggcagtagtt cccgctacga tcacgccatc tttccgaacg tcgagtacat aaccttcttc
     3121 gcccagcgag ggattgacag aaacgctcct gcttctcagg gcactttgta tgacgggttg
     3181 gtccaacttg ccgatgagta ttgccctgga tcttttgcca ttcgagactc gtagcacaat
     3241 cccagatgtt tgtttgacgt catcgataaa gtctgttgcc gcgaacttgt cttcgctcga
     3301 tttcgaatcg gccagtcgca gataggttcc acgatcgagc gcaaaggctt ctgcatttgc
     3361 cgtgacctgt cttggtttag ggataacggc aatagctgaa gagtcatcgg acgtttgcgc
     3421 gcgaacaagg ccattaacaa atagcagaaa caggctgacg acgatcagac gcgaacccaa
     3481 acttcgtgtt agagtgtttg ccatatttgt acttgtactt ggtcctttgt actttatatt
     3541 gcgctatgtg aggaaacgac cgtgtccgat atgctttagc ttgtcggggt tcccgcccgc
     3601 aagtgattca ttttcggcac gacaagctaa cgcatattgg acatcggaca tggtcgaatc
     3661 agaatgccga cgtaaagtac caagtacaaa gcacaaagta caaaggacca aacacaattt
     3721 cattgagagt tgagtctgac tgaaccgtgt ccttgatgct ttcggaaata actgagcaac
     3781 caaaagcgct cgcgaaaacc attagcgagg aacgaaataa actcgccagc ctcggaaaac
     3841 ttctaaagtc gcgtgagata gatttgatcg tcctggtggc gcgcggtagc tcagataacg
     3901 cgtcactatt tggtcgatac ctgctggaaa taaccaccgg gattcctgtc tctctatcag
     3961 caccatccgt ccacacaatt tacaacgcaa aactaaagct ggaccatgcg ttggtcgtag
     4021 gcgtttccca gtcgggagag ggcgaagaca tcaatcgagt cctggaaaac gcccgcagct
     4081 ctggagcatt tacagtcggc atcacaaacg agccaaactc gtctatgact aagctcgttg
     4141 acgagactct gctgacacac ggcggccgag agcgttcagt agcagcgact aaaacgttta
     4201 cgggtcaaat gctcctcttc tacatgctgg ccagcgagtt ggccagtggg aaactctcct
     4261 ttaattatga agtgatcccg gacttcgtcg aacgtgcatt ggagcagaag cctgcgattc
     4321 ttgagttggt acaacgttac gtattcatgg agaactgcgt cattgtcgga cgtggtctgg
     4381 catatgcgaa tgcctacgaa ctggcgctca aattgatgga gacgtgttac gtggtcgccg
     4441 agcgtttttc gtctgctgac tttcttcatg ggccactcgc gatggtggag cgacactttc
     4501 cggttattct atttgcgccg ccgggagtta tgttgccggg cgtgaagagt ttgatcgagc
     4561 ggcttcgaga acttcatgcg gacacgttgg ctatcacgag tgacttagat gctgcggcgc
     4621 tttgtagccg gtcgatagta atgccgaagg agatcgacga atttgtggcc ccgattcctt
     4681 atattgttcc aggccaactc tttgctgcgc tgttagccga ggcaaaaggc ctcaatccag
     4741 attcgcccag atcattatta aaagtaacaa ggacattgtg agcttccgat gaacttgtca
     4801 gtaccgattc gcggtagctt cgggtgagtc tccagatcat caagttgtgt tatccataga
     4861 cccgatggtg cggcacaaac cggagttagc cccaaaagaa tttgaaagag atctcccatg
     4921 aatatcgaat taaaagactt aagtcacaat ccggtttacc tacaagtccg ggaacagatc
     4981 gaaacgcaga ttaagaataa gagcattacc tccggcgaaa gtcttccttc acccggttc
//
LOCUS       DASSFP010000371         4143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_547617, whole genome shotgun sequence.
ACCESSION   DASSFP010000371 DASSFP010000000
VERSION     DASSFP010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547617"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VFH91_08325"
     CDS             complement(<1..1517)
                     /locus_tag="VFH91_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863040.1"
                     /translation="MRKRLTVGLLLSLTVLALVIVPLTDAGKDFRLNRVSELIKARAT
                     VQQPPRVTGYQYKAVNFATSAPLTELAKGQETVANPAAVEIKDKEEVIEEKREANSIP
                     NKTKFQTNGSDLDNAGEHEKNFKNREIIRNFDPDVKTAPDAAIATVPTTKGAGLSALA
                     IPTPSTSFEAISLTDTTALPGQGFLPPDTVGEIGPNHFVQMVNSSFRIYDRAGNPLIP
                     LTSIGTLFSSIPGPCANNIDGDPIVMYDQLADRWILSEFCTVANPNNHQLIAVSKTGD
                     PTGQYYLYDFMMPNNKFNDYPKFGVWSDAYYMTDNEFNQAGNQFLGAGVFAFDRTKML
                     SGDPAASFVYIDKAEGCPVCQFGGMLPADVDGFTPPPPGSPAPIIQFDANEFGATDSL
                     RIFDFHVDFATPANSTLTERTGSPLAVAAFDPREVPAGSRNVIPQPATTVKVDAISDR
                     LLYRLAYRNFGTHESLVMNHTVNVATNPAFRAGVRYYEIRKTSPTGAWTVQEQGT"
     gene            1717..2073
                     /locus_tag="VFH91_08330"
     CDS             1717..2073
                     /locus_tag="VFH91_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863041.1"
                     /translation="MRSDAQLTHNWRKFQKATPSAGGTGLAASFDLSGSLFTGSELEM
                     MAPANHEVGSEFRATIPRLSVTVNPLFRVFDFMCEDFTHDLNLHHGPDRNNRFHEIAN
                     ELMKSCFDPPYGRMYI"
     gene            2119..3510
                     /locus_tag="VFH91_08335"
     CDS             2119..3510
                     /locus_tag="VFH91_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET6863042.1"
                     /translation="MDVLANARERSRKVGTRVRYGTKRLKRISQLPTLGGIFLSFLKI
                     GLVGFGGGLAVIAQIRSLVVKQRRWFTDHEFAETFALAQSLPGTSAGNAATYIGFRLR
                     GWQGSAVALIGFILPSMVMMIILAILYRHLRAFPDTDRLFHGLNAAVVAVVLVSAFRI
                     GRSTLNRPWQWYVAVLSSIAVVVFGATIIEVVLAAGLIGIYIDSFGEKRWHRWRRISR
                     LALRRRAKLAELEHKNFVGGYLTRALAEERVRKQSEVDSDDLTEPEDSTHARLRSSSV
                     FLVAMPILAKLALLLALASVFLRMGAVTFGGGFVMIPLIESEVVRTNHWLTPQEFADA
                     TALGQMTPGPVLITATFIGYRVAGTLGALVSTVAVFLPSFLMTIAAGSSLRRFRSNRQ
                     VQSFLRGVTPAVVGLLVAAAWSVGRAGIHTWVGLTIVGLAAVVMWRFRVNPFWVILGA
                     GVLRFLVGLVMGA"
     gene            3540..3812
                     /locus_tag="VFH91_08340"
     CDS             3540..3812
                     /locus_tag="VFH91_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET6863043.1"
                     /translation="MDLRVLLTTFGIIFLAEMGDKTQLAAMTMAAQTKRPWAVFIGAS
                     CALAAVSALGVLVGSVIGDYVPLIWVKRVAAIAFVVIGVLMLLEKF"
     gene            3856..>4143
                     /locus_tag="VFH91_08345"
     CDS             3856..>4143
                     /locus_tag="VFH91_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET6863044.1"
                     /translation="MPQTPHIERHFTSSEVVRDIVIGMADGLTVPFALAAGLTGAIQA
                     TGIIVTAGLAEIAAGSIAMGLGGYMAAKSDAEHYAKEREREKKEVAEIPHEE"
BASE COUNT          931 a          980 c         1153 g         1079 t
ORIGIN      
        1 attgttccct gttcttgtac agtccaggct ccggtcggag aagtcttgcg aatctcgtaa
       61 tagcgcacgc cggccctgaa agcggggttg gtggcgacgt taacagtgtg gttcatcacg
      121 agcgattcgt gtgtaccaaa gtttcgataa gctaagcggt aaagcaagcg gtctgaaatg
      181 gcgtcgacct ttacggttgt tgccggctgc ggaatgacat tgcgtgaacc tgccggaact
      241 tctcgaggat cgaatgccgc gactgcgagt ggactgccgg ttcgttccgt cagcgtcgaa
      301 tttgctggtg tggcgaagtc aacgtgaaag tcaaaaatac gcagcgagtc cgtagcgcca
      361 aattcgttag catcgaactg aattatcggt gcaggtgatc ccggaggcgg tggggtgaag
      421 ccgtccacgt cggccggcaa catcccgccg aactggcaga ctggacaacc ctctgccttg
      481 tcaatgtaga cgaaacttgc ggccggatct cccgacagca tcttcgttct atcaaaagca
      541 aaaacacccg caccgagaaa ttgatttccg gcctgattga actcgttatc cgtcatgtaa
      601 taagcatcgg accagacacc gaacttcgga tagtcgttga acttattgtt cggcatcatg
      661 aagtcgtaca ggtagtactg gccagtggga tccccggttt tggagacggc gatcaactgg
      721 tggttgttgg gattggcgac tgtacaaaac tctgacagga tccatcgatc cgcgagttgg
      781 tcgtacatca cgataggatc gccatcgatg ttgttggcac atggaccagg aatcgaactg
      841 aagagtgttc caatgctcgt caagggaatc aaagggttgc cggcgcggtc gtagattcgg
      901 aaggagctgt tgaccatctg tacgaaatgg tttggaccaa tttcgccaac agtatcaggc
      961 ggtaaaaatc cctgtcctgg aagagcggtg gtgtcggtca gggatattgc ctcaaagctt
     1021 gtggaaggtg ttggaatcgc aagagccgac aggccggcac ccttcgtcgt tggcacggtg
     1081 gcaatcgcgg cgtctggggc ggtttttacg tctggatcga aattacggat aatttcacga
     1141 ttcttaaagt tcttttcgtg ctcgcccgca ttatcgaggt cgctgccgtt ggtctgaaat
     1201 ttcgtcttgt ttggtattga gttcgcttcg cgtttttctt cgattacctc ctccttgtct
     1261 ttgatctcaa cagcggccgg attcgctacc gtctcctggc ccttcgctag ttctgtgagc
     1321 ggtgcagagg tagcaaaatt aactgctttg tattgatatc cagtcacacg aggtggttgc
     1381 tgaacagttg cgcgtgcctt tatcagttct gacactcgat tcaaccgaaa gtcttttccg
     1441 gcgtccgtta agggaacaat aaccagggcc agaacagtca aacttaatag aaggccaacc
     1501 gttaagcgct tgcgcatttg tcgtaatctc ctttgagcgt tcaggtggga gctggattcc
     1561 ctcccacgta tcgagaaaca ttgattgctg tagagctaaa ccaaccgtgc tcgaaagact
     1621 ccggctacca ctgagaaaaa catcctcgct gtccgaagtt gagttttgag agttgttgag
     1681 actgagagat gacgagcagt tttctgccgg aaggaaatgc gcagcgatgc gcaactaact
     1741 cacaactggc gaaaattcca gaaggcaacg ccttcggctg ggggtaccgg gttagccgca
     1801 tcctttgact tgtcgggttc tctgttcaca ggctccgaac tcgaaatgat ggcgcctgcc
     1861 aatcacgaag tcggatcgga gttccgcgcg actataccga gactgagtgt gacggtcaat
     1921 ccactttttc gtgtgttcga ctttatgtgc gaggacttca cacacgatct aaatttgcat
     1981 catggtcctg atcgcaacaa tcgctttcat gaaatcgcca atgaattaat gaagagctgt
     2041 tttgaccctc cctacggacg aatgtacatt tgacctgaaa taagttacga agcataggga
     2101 ctcaatacag atttagctat ggatgtactt gccaatgcca gggagcgaag cagaaaagta
     2161 ggaactagag ttcgttacgg cactaaacgc ctcaagcgca tctcacagtt accaacgctc
     2221 gggggtatct ttctttcatt tcttaaaatt ggtcttgtcg gatttggtgg tggactagct
     2281 gtcatcgcgc agattcgctc gttagtagtt aaacagcggc gctggtttac tgaccacgag
     2341 tttgctgaaa catttgcgct tgctcagagt ttgccaggaa ctagtgcagg aaatgctgca
     2401 acctatatcg gctttagatt gcgtggttgg caaggatccg cagttgcact tattggcttc
     2461 atccttcctt caatggtcat gatgatcatc ctcgccattt tgtaccggca cctccgtgcg
     2521 tttccggaca cagatcgttt gttccatggt ctcaacgctg ctgttgtagc agtggttctg
     2581 gtctcagcgt tccgaatcgg acgaagtacg ctgaatcggc cgtggcaatg gtacgtggcg
     2641 gttttatcct caatcgcggt ggttgttttt ggcgcaacga tcatagaagt tgtactggca
     2701 gcaggtctca ttgggattta tatcgactcg tttggagaga aacgatggca cagatggcgt
     2761 cgcattagcc ggctggcact acgtcgaagg gcgaagcttg ccgagctcga gcacaaaaat
     2821 tttgtcggtg gctacctcac tcgtgcgctc gccgaggagc gtgtacgaaa gcaaagcgaa
     2881 gttgattccg acgacttaac cgaaccggaa gattcgacgc atgcacggct tagatcctca
     2941 tcagttttcc tggttgcgat gccaatactc gcgaagctgg cactgttgct tgcacttgct
     3001 tctgtcttcc tccgcatggg cgccgttacc ttcggcggag gttttgtcat gattccgctc
     3061 attgaatcgg aagttgtcag gaccaatcat tggttaacac cacaagagtt tgctgatgcg
     3121 accgcattgg gccagatgac tcctggtcca gtcttgatta cggctacttt tatcggttat
     3181 cgagttgccg gcacactggg agcgttggtc tccactgtag cagtctttct cccatcattc
     3241 ttgatgacga tagccgccgg ctcctcgctg aggcgcttcc gttcgaatcg acaggttcaa
     3301 tcgttcttgc gaggggtaac accggcagtt gttggattgc tcgtagctgc tgcatggagt
     3361 gtcggcagag ctggaattca cacctgggtc gggctgacga tcgttgggct ggcggcagtg
     3421 gtaatgtggc gattcagagt taacccgttt tgggtgattc tgggcgcagg cgtactgcga
     3481 ttcctggtcg gcttagtgat gggagcgtaa gtctaattgg ttagtaatag gagcataata
     3541 tggatcttcg agttttactc actacgttcg gaatcatttt cctcgctgaa atgggcgaca
     3601 agacccaact cgcggcaatg actatggcgg cgcaaacaaa acgaccgtgg gccgtattta
     3661 ttggtgcctc atgtgcactc gcggcagttt ctgcgctggg agttctagtt ggtagtgtta
     3721 ttggcgacta cgtcccgctc atctgggtga agcgtgtagc ggctatagct tttgtggtaa
     3781 ttggtgtatt gatgcttttg gagaagtttt gaaaaccgcg cgcattggac caccttgcca
     3841 gattaaggag aatagatgcc tcaaactccc catatagaac ggcattttac gtcgagtgaa
     3901 gtggtgcggg atatcgtgat tggaatggca gatggtctaa cggttccgtt cgccctcgca
     3961 gcaggattaa ccggagccat tcaggccacg gggattattg tcactgccgg cctggccgaa
     4021 atagcggctg gttccatcgc gatgggactt ggcggttaca tggccgcgaa gagtgacgct
     4081 gagcactacg caaaggagcg tgaaagagag aaaaaggaag ttgctgaaat tccacacgaa
     4141 gaa
//
LOCUS       DASSFP010000372         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_999233, whole genome shotgun sequence.
ACCESSION   DASSFP010000372 DASSFP010000000
VERSION     DASSFP010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999233"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFH91_08350"
     CDS             <1..828
                     /locus_tag="VFH91_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HET6863045.1"
                     /translation="GGTGVLMQGGLHPDFGIEWYEDLLRSLRARYPNFQLHCFSPPEI
                     HNIHLISKLDYETIMRRLKDAGLYSLPGGGAEILDDEVRKRVATKCTTDEWLSVMRAV
                     HRVGLRSTATMMFGIGDKVEHRVRHLQRIRDLQDETGGFTAFIPWTFQRENTALGRKI
                     KDEPTGMDYLKMLSVSRLFLDNVENFQSSWLTQGLRLGQVALRFGANDMGSIMIEENV
                     VSAAGANNEADEKMLQFLIREAGFIPQQRDILYRHVNRSQLAQSETPGGEAQPVMTL"
     gene            complement(902..2095)
                     /locus_tag="VFH91_08355"
     CDS             complement(902..2095)
                     /locus_tag="VFH91_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6863046.1"
                     /translation="MGESHYDIVTVGGGLGGCSLAKVMAEHGKRVLVLEREQNFRDRV
                     RGEFMTPWGVAEVRSLGIYELIRDNCGIDAQTSQLGFGPRDLPSTTPQQLPGLGFSHP
                     EMQEVLLDAAKNGGAEVRRGVTVTGIKPGATPAVTFLDENGDDQEVNARLVVAADGRT
                     SSARKWAGFEVKESPQPFYFAGLLLKGVAAPEDEVYLLFLPHVGACTAMTPVGRGRFR
                     TYVAYGDSEGMRLQGEHNISNFLADARRAELVADYFHKAEPIGPLASFRCGDFWVEHP
                     FKDGVALVGDAGSTSDPAFGQGLATTARDVRVLRDSLLANDDWDAAGHAFATEHDRYS
                     QVIRRVSQWFRSLFLEQGVDADARRARAMPLIAQDETRVPDHLFSGPDLASDDSVRRR
                     FFGES"
     gene            2208..2855
                     /locus_tag="VFH91_08360"
     CDS             2208..2855
                     /locus_tag="VFH91_08360"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6863047.1"
                     /translation="MTYYDITVPLINEMPTYPGDPGIKITDWRSLANGDAANVSHMNF
                     GAHTGTHIDAPAHFIKGAPKIESLDLEVLIGEATVVEVPDNYLSIDADFVDQHLPPAG
                     PIRVLFKTRNSAFWSKPDGPFRTDFTYLELSAAKKLVERNVKLIGIDYLSIEKFAQEN
                     HETHLELLSNGVVIVEGLNLASVPPGNYELVCLPLRIRSGQGDGSPARAVLRTLQ"
     gene            2867..3259
                     /locus_tag="VFH91_08365"
     CDS             2867..3259
                     /locus_tag="VFH91_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET6863048.1"
                     /translation="MAAKRDQENYRQRVYQIVRKIPRGRVMTYGQIAEILGEGYTPRT
                     VGFCMHSCPEGTPWQRVLNAQGGYSTGRLVLPHDKQQRLLELEGVKFNKSHRCDLQTY
                     LWIPEIHAKPDKKVSLFKKGAKQRGAKR"
BASE COUNT          837 a          909 c          865 g          817 t
ORIGIN      
        1 ggcggcactg gcgtactgat gcaaggtggg ctacatcccg attttggtat cgagtggtac
       61 gaagatctgc tgcggtcgtt acgcgccagg tatcccaact ttcaattgca ttgcttctca
      121 cctcccgaga tacataacat ccaccttatc tctaaactgg attatgaaac gatcatgagg
      181 cgactgaaag atgcaggcct ttacagtctt ccaggtggtg gtgccgagat acttgatgac
      241 gaagtgcgaa agcgtgtggc tacaaagtgc actactgacg agtggctctc tgtgatgcgc
      301 gccgtccatc gcgtcggatt gcgttcaaca gcgacgatga tgtttggcat cggagataag
      361 gtcgagcatc gagttcgaca tctacaacgt attcgtgatc ttcaggatga gaccggaggt
      421 tttaccgcct tcattccgtg gacattccag agagaaaata cggcactcgg acgcaagatt
      481 aaggacgagc cgacgggtat ggattacttg aaaatgcttt cggtgtctcg attgttcctg
      541 gataacgtcg aaaactttca atcgtcatgg ctcacacagg gtctgcggtt gggtcaggtg
      601 gcgctcaggt ttggcgctaa cgatatgggc tcgatcatga ttgaggaaaa cgtagtctcg
      661 gctgcaggtg cgaataatga agcagatgag aagatgctac agtttttgat tcgtgaagcg
      721 ggtttcattc cccaacagcg ggacatcctt tacagacacg tcaatcgttc acaactcgca
      781 caaagtgaaa cacctggtgg agaagcccag cccgttatga ctttgtaggg gcgtccctcc
      841 gtggacgccc tctttcgaag tgtcttttca atcacgagcg gggcgcccag gaaaagacgc
      901 cctacgattc accaaagaat cttcgtcgca ctgaatcatc cgacgctaag tccgggccac
      961 tgaatagatg gtctggaacc cttgtttcat cttgagcaat caacggcatc gcacgtgccc
     1021 tacgtgcatc tgcatccact ccctgttcca aaaatagact tctgaaccat tgcgatactc
     1081 ttcgaattac ttgcgagtag cgatcgtgtt ccgtggcgaa cgcatgaccg gccgcatccc
     1141 aatcatcatt cgccaacaaa gaatctcgca gcacccgaac atcgcgggcc gtagttgcca
     1201 atccctgacc gaaggctgga tcactcgtcg aaccggcatc gccaacaagt gcgactccat
     1261 ctttgaaagg atgttcaacc caaaaatcgc cgcagcgaaa agaggccagt ggtccaatgg
     1321 gttctgcctt gtgaaagtaa tcggcaacca attcggcgcg tcttgcgtct gccagaaaat
     1381 ttgatatgtt gtgctctccc tggagccgca ttccttcact atcgccatat gccacgtagg
     1441 ttctaaaacg gccgcggcct acgggagtca ttgcagtaca ggctccgacg tggggcagga
     1501 agagtagata gacttcatct tctggagcgg ctacgccttt tagcaagagg cccgcaaaat
     1561 aaaatggctg gggcgattct ttcacctcga agccggccca tttccgagcc gaggaggttc
     1621 taccgtcggc cgcgacgacg agcctcgcgt taacctcctg gtcgtcgcca ttttcgtcca
     1681 ggaaagttac ggctggagtt gcaccgggct tgattcctgt gacagtcact ccccgtcgga
     1741 cttctgctcc cccatttttc gctgcatcga gcagaacttc ttgcatctcc ggatgcgaga
     1801 agcccaggcc gggcaattgc tgaggggtag tgctgggaag atctctcggt ccaaagccaa
     1861 gctgggatgt ttgagcatca attccgcaat tgtcgcgaat gagttcatag atcccgagcg
     1921 acctgacttc cgctactccc cacggcgtca taaactcacc gcgaacacgg tcgcggaaat
     1981 tctgttcgcg ctcgagaacc agaacgcgct ttccatgttc tgccatgact ttggcgagtg
     2041 aacaacctcc taatccacct ccgactgtaa cgatgtcata atgtgattcg cccatgtttc
     2101 cgcctctctg ttgaatgtgc ggaaatgcta ctccggtgat cgggaaaatg gaatggatag
     2161 gacctatttg acctgtagga cctattcact ctagtctaaa attaaccatg acttattacg
     2221 acatcactgt cccgctgatt aatgagatgc cgacttatcc cggcgatccg ggaattaaga
     2281 tcaccgactg gcgaagtctg gccaacggag acgcagccaa tgtatcgcac atgaattttg
     2341 gcgcgcatac cggaactcac atcgacgcac cggcgcactt cattaaagga gctccgaaga
     2401 ttgagtctct tgatcttgaa gtgctgatcg gcgaagcaac ggtcgtcgag gtgcctgaca
     2461 actatctgtc tatcgatgct gattttgtcg atcaacatct tcctcccgcc ggcccgattc
     2521 gggttctttt caaaaccaga aactctgcgt tttggtccaa gcctgatggc ccattccgta
     2581 cggactttac ctatctcgaa ctcagcgctg cgaagaaact cgtagaacga aatgtgaaac
     2641 ttattggtat cgattatcta tcgatcgaaa aatttgcgca ggagaaccac gaaacacatc
     2701 ttgagcttct ctcaaacgga gtcgtcatcg ttgaaggctt gaatctcgcc agtgttcctc
     2761 cgggaaatta cgaactggtc tgccttccac tgagaataag aagcggacaa ggcgatggct
     2821 ccccagcgcg agcagttctt agaacgcttc agtaaaggcg taaatagtgg ccgcaaaacg
     2881 agatcaggaa aactaccgtc aacgcgttta ccaaattgtc cggaaaattc ctcgtggtcg
     2941 cgtcatgacc tacgggcaga ttgctgagat tctcggtgag ggttacacgc cccggactgt
     3001 tggtttttgc atgcactcgt gtcctgaagg aactccctgg cagcgagttc tcaacgcgca
     3061 gggcggctat tcgactggcc gactcgtact accacacgat aaacagcagc ggctacttga
     3121 gttggaaggc gttaaattca acaaatcgca ccgctgcgat ttacaaacat atctttggat
     3181 acccgagatt cacgctaagc ctgataagaa agtatctctt tttaaaaagg gagctaagca
     3241 aagaggagcc aaaagatgag ttcagacccg atgcacaatg ttccatacgc cgtaagccca
     3301 ccgcgttact cagtgtcgca ccagatggtt acgaccgctt ttgaccttcc caactaccgg
     3361 gtcactcaga acctcggcgt tgtgcggggg atcgttgttc gttcgcgaaa tatctttgca
     3421 acgatcgg
//
LOCUS       DASSFP010000373         6327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_548180, whole genome shotgun sequence.
ACCESSION   DASSFP010000373 DASSFP010000000
VERSION     DASSFP010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548180"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VFH91_08370"
     CDS             complement(<1..1016)
                     /locus_tag="VFH91_08370"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HET6863049.1"
                     /translation="MSALAERRGWSATAFLPSDHEFQKLLNNSSLAPDSLLDYLRSRA
                     EPRFFASFDNRVLSIETFKRKWPTTEQSLIDRAQRAVDGEFDLLGHSALAFGNPVDWH
                     LEPISGKRSPLVHWSSVEELDSEASGDKKFVWELNRHQHFVLLGQAYLFTKDERYATA
                     FMDQLESWMDQNPPKMGTNWLSSLEIAFRSISWVWGLNFFLHANSLRPDVVWRAFKFI
                     YLNARHIENYLSTYFSPNTHLTGEALGLFYIGTLFPEFRDAGRWRSLGRQILLEQLPI
                     HVLDDGVYFEQSSYYHRYTADFYLHFLLLARLNRDELPQDVSQRLQSLLDHLMYITRP
                     DGTMP"
     gene            complement(1135..2319)
                     /locus_tag="VFH91_08375"
     CDS             complement(1135..2319)
                     /locus_tag="VFH91_08375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6863050.1"
                     /translation="MSSQNQKLDAENKEPLRVLVLAPSLDILGGQSRQCARLLGHLQD
                     EPSLRVDFLPHNPRMPGFLKWTQKIKYVRTVFTTTLYWLLLVLRAYRYDILHIFSASY
                     YSYLLSVAPAILLGRLYGKKVILNYRSGEAEDHLANWKLSAVPTMRLAHAIVVPSGYL
                     VEVFGRFGLKAVPIHNIVELERFKFRDRTPLRPVFLTSRLLEPLYNVGCVLRAFAVIQ
                     RQFPNASLTVAADGWMRGELEQLARDLGLRETKFIGSVAFADMPAMYDSADVYLTATN
                     LDNMPSSITESMASGLPVVTTNAGGIPFIVTHEETCLMVACNDHEAMAREAVRLLQNN
                     ELARKIGTQARVSAKKFTWPVVRDEWVKLYRKLGSRESLTETHWEGQPGNGTPVEFGP
                     TS"
     gene            complement(2322..3530)
                     /locus_tag="VFH91_08380"
     CDS             complement(2322..3530)
                     /locus_tag="VFH91_08380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6863051.1"
                     /translation="MSINVLQLIGSFNQGGSERQAVQLTRLLREDGRFNVLVATLDSS
                     GPLGSQVRRLGLNDVPEYPLTSFYDRNAVAQMKRFVKYLKQSDIHVVHAHDFYTNIFG
                     MTASSLARVPVRIASRRESSVRVARQRFVERGAYKLAQKVVANCDEVRLQLIAEGVSA
                     AKVTTLYNGLELSSFVQNKDNDRNELLRSLRLPVDVPRRFITIVANLRAHFQEPTPVC
                     LKDHPTFLRAARRVREAIPDAAFVIAGEGEMLDDTRKLAAQLGIEQETFFLGRCENVP
                     ALLSASFACVLSSTAEGFSNSILEYMAAGRPVVATRVGGAAEAIEEGISGSLVNAGDD
                     ESMAERLIRLLSNSTKAEEMGRRGREIVREKFSSEAQLRRTTNLYDELLNRQSRELVG
                     AVGSARRGSA"
     gene            complement(3534..4403)
                     /locus_tag="VFH91_08385"
     CDS             complement(3534..4403)
                     /locus_tag="VFH91_08385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6863052.1"
                     /translation="MVADSRQVSTHQAEVSTGERFAFGENWARFLAVLDDKRIDEAVA
                     SLKENLEVATLEGTSFLDIGSGSGLFSLAARKLGARVHSFDFDPNSVACTAELKRRYF
                     AGDTNWTVQEGSALDREFVASLGQFDVVYSWGVLHHTGQMWKGLENAAIPVAAGGKLF
                     IAIYNDTGTQSLRWRWIKKTYNQLPGFLRSPFAVLAIAPDEMKSAIRATIKLRPQQYI
                     RSWTNYDQNNRGMSRWHDIIDWVGGYPYEVATPEEIFNFYRKRNFSLTKLKSGKVGLG
                     CNEFVFQKEQAAK"
     gene            complement(4419..>6327)
                     /gene="asnB"
                     /locus_tag="VFH91_08390"
     CDS             complement(4419..>6327)
                     /gene="asnB"
                     /locus_tag="VFH91_08390"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6863053.1"
                     /translation="GAEEAGAILDRMCQVIAHRGPDDQGVMHTSDGVHLGMRRLSIID
                     LAGGHQPISNEDGQVSIVFNGEIYNYRELYPELKTRGHQFKTHSDTEAIVHAYEEFGV
                     DCLKPLRGMFVFAIWDAKNKRLFMARDRVGKKPLYYTLTDDSTLIFGSELKSLLQHPK
                     TRRVVNPEAVDAYLTCGYVPDPLTILRDVHKLPPGHYLTFENGKIDVRSYWDFQYGEP
                     GNSASRKEEDYLDELRSLLAESVKLRLIADVPLGAFLSGGIDSSSVVGLMSKEMSQPV
                     KTFSIGFQEDSYNELKYARIAAKHFQTDHHEFVVTPDLCEIVDELAWFLDEPFADSSA
                     IPTYMVSKMARQYVTVVLSGDGGDELFGGYTRYVIDRQRSAFGQLPRFLRRGVMAPLS
                     KQLPHGARGRNFLHNIALDPLDRYLDSISIFTELNKPRLYSDGFADQLERENRSGSGV
                     LRSWRTYASNVKTGHDLDKMLYVDSKTYLPGDILTKVDRMSMANSLEARVPLLDHKLI
                     EFVNRIPAGLKLKGLETKYIFKRAIEDFVPAEILHRPKQGFGVPVQEWINEQLREKIH
                     DTLTEKRTIERGYINRQYVNLLLDEHERGRRDHSTQLWTLFMLELWHRSVYEYTADTS
                     NRIASEVVEVA"
BASE COUNT         1605 a         1687 c         1514 g         1521 t
ORIGIN      
        1 ggcatggttc catcgggacg tgtgatgtac atcaaatgat cgagcaagct ctgtaagcgc
       61 tgcgaaacat cttgcggtaa ttcatcacga ttgaggcggg ccaataaaag aaaatgcaga
      121 tagaaatcgg ctgtatatcg gtggtaataa ctcgactgct caaaatacac accatcatca
      181 agaacgtgaa ttggcagttg ctcaagcagg atttgcctgc ctagagaacg ccagcgtccc
      241 gcatccctga attccggaaa caatgttcct atataaaaga gcccgagagc ttcacctgtt
      301 agatgtgtat ttggactgaa ataggtggaa agatagtttt caatgtgtct ggcgttgaga
      361 tagatgaact tgaaagcccg ccacaccaca tccgggcgaa gcgagtttgc atgcagaaag
      421 aaattgagac cccaaaccca tgaaattgag cgaaacgcga tctcaaggct actaagccag
      481 ttggttccca tttttggagg attctggtcc atccatgact cgagctggtc catgaaagcc
      541 gtcgcatagc gctcgtcctt cgtaaacaaa tatgcctgac ctaacagtac gaagtgctgg
      601 tgccggttaa gttcccacac gaacttctta tcacccgaag cctcagagtc aagttcttct
      661 actgaactcc aatgaacgag cggcgagcgc ttgcccgata ttggctcgag atgccaatca
      721 acaggatttc caaacgcgag ggccgaatgt ccgaggaggt caaactcccc gtccacggct
      781 cgctgtgcac gatcgatcaa tgactgttca gtggtgggcc atttacgttt gaaagtctcg
      841 atactcaaca cccgattatc gaaggaggcg aagaaacgag gctcagcccg tgaacgtaga
      901 taatccagaa gagagtcagg agcgagtgaa gagttattga gaagcttttg aaattcgtgg
      961 tcggacggca aaaaagctgt cgctgaccac ccgcgacgtt ctgcgagggc cgacaacttt
     1021 tgttttcctc taacgcggag ttcgtcgagt ttgattgtcc gaaactttac caatcgctat
     1081 cagtccggca aggtggaagt tgcggttccc ttaaaagaca ttggaggttt cttttcaaga
     1141 cgtgggtccg aattcaaccg gcgttccgtt tcccggctga ccttcccaat gcgtctcggt
     1201 caacgactcg cgtgatccca gtttgcggta taacttgacc cattcgtctc ggaccacagg
     1261 ccaggtaaac tttttcgccg aaacgcgagc ctgggtacca attttgcgag cgagctcgtt
     1321 gttctgtaat aatctgactg cttcacgcgc catcgcctcg tgatcgttgc aagcgaccat
     1381 caaacatgtt tcttcatgag tgacgataaa ggggatgcca ccagcattcg tcgtcacaac
     1441 tggaagtcct gaagccatgc tttcggtaat tgaactgggc atgttgtcga ggttcgtggc
     1501 ggtcaggtac acgtcggccg aatcgtacat cgccggcata tcggcaaaag ccacacttcc
     1561 aatgaacttt gtttcgcgca atcccagatc acgtgcgagt tgttctagtt ctcctcgcat
     1621 ccaaccgtcg gcggcgacgg ttaaactggc attggggaat tgcctttgga tcactgcaaa
     1681 ggccctgagc acacaaccaa cattgtacag gggttcaagt aagcgactgg ttagaaaaac
     1741 cggacgcagt ggagttcgat cgcgaaactt aaaccgctca agctctacta tattgtggat
     1801 cggtactgcc tttagtccga atctaccaaa cacttcaacc agataacccg acgggacgac
     1861 gatagcgtgg gccaatcgca tcgtaggcac agctgataac ttccaattag caaggtggtc
     1921 ctcagcttca ccactccggt aattcagtat taccttctta ccgtataacc tacctaacag
     1981 aattgccggg gcgacggaga gcagatacga ataataagag gccgaaaaaa tatgaagaat
     2041 atcgtaacgg tatgctctta aaacaagcag caaccaatac agagtggtag taaaaaccgt
     2101 gcggacatat ttgatcttct gcgtccattt gaggaagcca ggcatgcgcg ggttgtgcgg
     2161 caaaaagtca actcttaatg agggctcgtc ttgaaggtgg cccaacaacc tggcgcattg
     2221 ccgggattgc cctccgagaa tatccagcga cggcgccagc acaaggaccc gcaagggttc
     2281 cttgttttca gcgtcaagct tttgattctg actcgacacc actatgcgct ccctctcctt
     2341 gcactgccga ctgctccgac caactctctt gattgacggt tcaaaagttc atcgtagaga
     2401 ttggtggttc gtctcaactg cgcttcgctt gaaaatttct ctcttactat ttctctacct
     2461 cgccggccca tctcttcagc tttcgtactg ttacttaaca acctgatcaa tctttcagcc
     2521 atcgattcat cgtcgccggc gtttaccaga gagcctgaaa ttccctcttc aattgcttcg
     2581 gcagcgccgc cgactcgggt ggcgacgaca ggtcgtccag ccgccatgta ctcgagtatt
     2641 gaattcgaga agccctcggc agttgaactg agcacgcagg caaaagaggc tgaaagcaaa
     2701 gcaggaacat tttcgcaacg gccgagaaaa aaggtctcct gttcgattcc aagctgcgcc
     2761 gctaacttcc gagtgtcgtc caacatctct ccttcaccag caatcacgaa cgcggcgtca
     2821 ggtattgctt cgcgaacgcg tcgggcggct cttaggaaag ttggatggtc cttcaaacat
     2881 accggagtgg gttcctggaa atgggccctc aggttggcaa caatagtgat aaaccggcgc
     2941 ggaacatcga ctggcagtcg taacgatctc agtaactcat tacgatcgtt atccttattc
     3001 tggacgaaag aactcaactc caaaccgttg taaagcgttg tgacttttgc agctgagact
     3061 ccttcggcga ttaactgcag acggacctcg tcgcaattgg caaccacctt ttgggccaac
     3121 ttgtaagcgc cacgctctac gaaacgctgc ctggcaacac gcacggatga ttcgcgacgc
     3181 gaggcaatac gcaccgggac acgcgcaagt gacgaggccg tcataccgaa aatgttggta
     3241 taaaaatcgt gggcatgcac gacgtgaata tcactttgtt taaggtactt cacaaaacgc
     3301 ttcatttgag cgacggcatt gcggtcgtaa aaacttgtga gcggatattc cggaacatcg
     3361 ttgagtccta gccgccggac ttgcgaaccg agtggaccac tgctgtcgag cgttgcgacg
     3421 agaacgttga agcgaccatc ctcgcgtaac agacgcgtca actggacagc ctggcgctct
     3481 gagcctcctt gattaaaact gccgattaat tgaagaacgt taatactcac tagttatttc
     3541 gccgcctgct ctttctgaaa cacaaactca ttgcaaccta agccgacttt gccggatttg
     3601 agtttggtca ggctaaaatt tcgtttacgg tagaagttga atatctcttc cggcgttgcc
     3661 acttcataag ggtaaccacc tacccaatcg ataatgtcat gccagcggct cataccccgg
     3721 ttgttttgat cgtaattggt ccaggaacga atgtactgtt gaggtcgtaa cttgatcgtc
     3781 gctcgaattg ccgacttcat ctcatccgga gcaatcgcca gaaccgcaaa aggagacctc
     3841 aagaatcccg gtaactgatt gtaagtcttc ttaatccatc gccagcgaag actttgagtg
     3901 ccggtgtcgt tatagatggc gataaatagt tttccaccgg ctgcgaccgg tatcgcagca
     3961 ttctcgagac ccttccacat ctgcccggtg tgatgcagta ctccccatga atagaccaca
     4021 tcgaactgac ccaatgaagc gacaaactca cggtccaacg ccgaaccttc ctgtacggtc
     4081 cagttggtat cgccggcgaa atatcgtctc ttaagttcgg cagtgcaagc tacggagtta
     4141 ggatcgaaat cgaaagagtg gacccgggct cccagctttc gcgcagccag gctgaacaga
     4201 ccacttcccg aacctatatc aaggaagctc gtaccttcca gcgttgcaac ttccagattc
     4261 tctttcaaag atgccaccgc ttcgtcgatg cgcttgtcgt cgagcacagc caggaaacgg
     4321 gcccagtttt cgccaaatgc gaatcgttcg ccggttgata cctcagcttg atgcgtactc
     4381 acttgtctac tgtcagccac catttaattc attcctgctc aagcaacttc aactacttct
     4441 gaggcaattc tattactcgt gtccgccgtg tactcgtaaa ctgaacgatg ccataactcc
     4501 aacatgaaca aggtccaaag ttgagtggaa tggtcacgac gaccgcgctc atgctcgtcg
     4561 agaagtaaat tgacatattg gcgattgatg taaccgcgct caattgtgcg cttctctgtc
     4621 agcgtatcgt ggatcttttc tcgcagctgt tcgttgatcc attcctgaac gggcacaccg
     4681 aaaccctgct tgggcctgtg aagaatctcg gctggaacga aatcctctat ggctcgtttg
     4741 aaaatgtact tagtttccaa ccctttcaac ttaaggccgg ctggaatccg atttacaaac
     4801 tcgattagct tgtggtccaa caatggcact cgtgcttcaa gcgagttagc catactcatg
     4861 cgatcgactt tcgtcaggat gtcgccgggc aaataggttt tgctatcgac atacagcatc
     4921 ttgtcgagat cgtgtccggt cttcacattg cttgcgtacg ttcgccacga gcgaagtacg
     4981 ccagaaccag aacgattttc ccgctcgagt tgatccgcga acccatcgct ataaagccgc
     5041 ggcttattga gctccgtgaa aattgaaatg ctatccaggt aacgatcaag aggatcgagc
     5101 gcgatgttat gcagaaaatt ccgaccgcgc gcgccgtgtg gcaactgttt actcagcggc
     5161 gccatcactc cccggcgcag gaagcgaggc aactgaccaa acgcgctacg ttgacgatcg
     5221 atcacatagc gagtatagcc tccaaataat tcgtcgcctc cgtcgcctga caaaactacg
     5281 gtcacatatt gccgcgccat cttcgagacc atgtacgtag gaatcgcaga agaatccgcg
     5341 aatggttcgt ccaaaaacca cgccagctca tcgacgattt cacaaagatc cggcgtgacc
     5401 acaaattcat gatggtcggt ctgaaaatgc ttagcggcaa tgcgagcgta ctttaattcg
     5461 ttataactat cttcctgaaa tccaatcgaa aacgttttga ccggctgcga catctctttt
     5521 gacatcaacc ctacgacact actcgagtcg attccaccag acagaaatgc acccaacgga
     5581 acgtctgcga tcaaacgcaa ctttaccgac tcagccagca acgatcgcaa ctcgtcgaga
     5641 tagtcttcct cttttcggct cgctgaattt cccggttcac catactgaaa gtcccaatag
     5701 ctccggacat ctatcttgcc gttttcaaat gtcaggtaat ggcccggcgg cagtttatgc
     5761 acatctcgaa gaatcgtgag cggatccgga acatagccgc aggtaagata cgcatcgact
     5821 gcttcgggat ttacaacccg gcgagttttc ggatgttgca atagtgactt cagctcagag
     5881 ccaaagatga gcgtcgagtc atccgttaaa gtgtagtaaa gcggtttctt ccccactcgg
     5941 tcccgggcca tgaacagacg tttgttcttt gcatcccaga ttgcaaaaac aaacatgccc
     6001 cttagcggct taagacagtc aacaccaaat tcttcgtacg catgaacgat cgcttcagtg
     6061 tcagagtgag tcttgaactg atgtccgcgc gtcttaagtt caggatagag ttctcggtag
     6121 ttgtatatct ctccattgaa gacaatgctc acctggccat cttcgttgga gattggttga
     6181 tgtccgcccg caaggtcgat gatcgacaat cggcgcatac cgaggtgcac tccgtctgag
     6241 gtgtgcatga ctccttgatc gtcaggtcca cgatgcgcaa tcacctggca catacgatcg
     6301 agaatcgcac ccgcttcctc agcgccg
//
LOCUS       DASSFP010000374         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1301791, whole genome shotgun sequence.
ACCESSION   DASSFP010000374 DASSFP010000000
VERSION     DASSFP010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301791"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..501
                     /locus_tag="VFH91_08395"
     CDS             154..501
                     /locus_tag="VFH91_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6863054.1"
                     /translation="MAELDVKERQAGDVTILDLSGAVRIGEGSIALRDAIRSLADEGK
                     KKVLLNLRGVNYVDSSGIGELIANYTTIKRQGGQLKLLNLTDRIQNLLVITKLLTVFD
                     AYDNEPEALKSFE"
     gene            563..1030
                     /locus_tag="VFH91_08400"
     CDS             563..1030
                     /locus_tag="VFH91_08400"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET6863055.1"
                     /translation="MFRSAIRRLLAIDDPPERTALAFSIGVFIAFSPFLGLHTILATL
                     IAFIFRFNKVAIYTGTFINNPFLTLVPIIVASYGVGAFILGRPLHIPQEGVDLLKDPE
                     IFSGSYYRRLFWQSRYIVEPFAVGGMLLSVVCSVIAYPLTLRALRLYRTRRHG"
     gene            1124..2950
                     /locus_tag="VFH91_08405"
     CDS             1124..2950
                     /locus_tag="VFH91_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863056.1"
                     /translation="MSQFLLSALLLLSLSGIISAQTPASSPRPTQQQTRPDRQPIAFD
                     LSEYGVQFEADPRLIVVMAALEMAGLDSVPSGREPSAFRSKVRKDLANLDPDLRQRLR
                     NFYERNRLAAPATAADQASRYVSLALALGPPPGLEAPLRSEDLPAGLLEVLDFAPLVQ
                     EFYRRSGIDEQLVNYTRAYQAEGDRLRVPTAEMVRSILSYLHTRPITLTNERVEVKNT
                     GNKKSDSKVKRYTLHQKDRRFRVMPDLLAAPGTINFRVIGDDYYAIVPEGTEPGSSEL
                     IRAYLQYVIDALVLRFNKEIADRREQVKLVLDERQKAGAEVSPDVFLSVTRSLVAAAD
                     ARYNEQRRLEILAVNVRRQLQAAKDDATRAAITKSTQAQVAEIKDETVARLADEYEKG
                     AVLSFFFADQLKGIETSGFDIANFFPDMIASFDPAREIKRPAEYAEARQRAVVARAAR
                     AAARKAESDLNSSPENVRDAGLVKELTTVEQTLRNKDYNDAEAKLKSMLASYPREPRI
                     FFALGQTASLAAMDATDETIRDERLNRALGHYRLAVQSASPETDKAVLSRAHESMGRI
                     NAFLDNKVEAAREFDEAIKIGEVSGGAYKEAVEGKKKLAQPE"
BASE COUNT          842 a          783 c          844 g          840 t
ORIGIN      
        1 ggtcagttga agttattgaa tctcactcag aagcttcaag acttgctgac gattaccaaa
       61 ctccttacgg tctttgacgt ttatgagagt gaagccgagg ccctgaatag ttttaaataa
      121 gacctgctcg ggtcgaagca gacggagtaa gaaatggctg agctggatgt gaaagagcga
      181 caagccggag acgtcaccat tctagatctg agtggtgcgg tccgtatcgg cgaaggtagc
      241 atcgctttac gagatgccat tagatcgctg gccgacgaag gaaagaagaa agtcttactt
      301 aatctacgcg gcgtaaacta tgtcgactca agtggaattg gcgagttgat tgccaactac
      361 acgaccataa agcgccaagg cggacagtta aaactattga acctgactga tcggatccaa
      421 aacctattag tcataacaaa gttgcttacc gtttttgacg cttacgacaa tgagcctgaa
      481 gccctgaaga gttttgaata gacaggcaaa cgtgtctgtt tagtttgcgt cgaagctgac
      541 agaccagtaa gcctgtttac acatgtttcg tagcgctatt cgccgtctgc tcgcgatcga
      601 tgatccacct gagcggacgg cgcttgcctt ttcaataggc gtttttatcg cattttcccc
      661 tttccttggt ctgcacacca ttcttgctac gctgattgca ttcatctttc gcttcaataa
      721 agtcgcaatc tataccggaa ctttcatcaa caatccattt ctgacacttg tgccaattat
      781 cgttgcatca tacggagtag gcgccttcat tctgggacgt ccgctgcata ttccgcagga
      841 aggtgtggac cttttgaagg atcctgagat cttttccggt tcgtactacc gccgcctctt
      901 ctggcagagc cgttacatag ttgagccgtt tgccgttggc ggcatgcttc tctcggtggt
      961 ttgttcagtt attgcatatc cgctaacatt gcgagcgttg cggttatatc ggactcgacg
     1021 gcacggctaa tcgtctcttc accgtgttgg catcgaactt acttgcatag cgcatctatt
     1081 ttttctcgac taaacctgtt cgcaatttca acatttaatc gatatgagcc agtttttatt
     1141 aagtgcttta ctgcttcttt cactgtcagg aatcatctct gcgcaaactc ccgcgagttc
     1201 cccgcgccct actcagcagc aaacgaggcc cgaccggcaa ccaatagcct tcgatctaag
     1261 cgaatatggc gttcagtttg aagctgatcc cagattgatt gtggttatgg cggcgctgga
     1321 aatggccgga ctcgactccg ttccaagtgg ccgagaacca tccgccttca gatcaaaagt
     1381 tagaaaggat ctggcgaacc tggatccgga tttgagacag cggctgcgca acttctatga
     1441 gcgaaaccgt ctggccgcgc ccgcaactgc ggcggaccag gcctctcggt atgtttcgct
     1501 tgcgctggct ttgggtccac cgcccggact tgaggctcca ctccgttcag aagatctgcc
     1561 ggcaggattg ttagaggttt tggattttgc gcccctggtt caggaatttt accgaagatc
     1621 cgggattgac gagcagttgg tgaattacac gcgtgcctat caagctgagg gtgacagatt
     1681 aagagtaccg acagccgaga tggtcagatc aatcctaagc taccttcata ctcggcctat
     1741 aaccctgact aacgagcgtg ttgaagtaaa gaacactgga aacaagaaga gcgacagcaa
     1801 agttaagaga tacacccttc atcagaaaga ccggcgcttt cgggtgatgc ctgatttgct
     1861 cgctgctcct ggcaccatca atttccgggt tataggcgat gactattacg cgatcgttcc
     1921 tgagggcacc gaaccagggt cttctgaatt gatccgagct tatcttcaat atgtgattga
     1981 tgcgcttgtg cttcgcttca ataaggaaat tgctgatcgc cgggagcagg ttaagttagt
     2041 gctggacgaa cgacagaaag ccggagccga ggttagtccg gacgtttttc ttagcgtaac
     2101 ccgttcatta gtggcggctg ctgacgccag gtataacgaa cagcgaagac tcgagatact
     2161 cgcagtaaat gtccgccgtc aattacaggc tgcaaaggat gacgcgacgc gagcagcaat
     2221 taccaaatca actcaagctc aagtggctga aatcaaagat gagacggtgg ctcgacttgc
     2281 cgatgaatat gagaagggag ctgtgctgtc tttcttcttt gccgatcaat tgaagggaat
     2341 tgagacgtcg ggttttgata tcgcgaattt ctttccggac atgatcgcct catttgatcc
     2401 agctcgcgaa ataaaacgtc cagccgagta cgctgaagcg cggcagcgag cagtagtagc
     2461 tcgagccgct cgcgctgctg cgcgcaaagc cgaatctgac ttaaacagct ctccggaaaa
     2521 cgttagagat gccggtttgg taaaagagtt gactacggtt gagcaaacgt tgcgcaacaa
     2581 ggactacaac gacgcggaag cgaagttgaa atcgatgctg gcaagttacc ctcgtgagcc
     2641 aagaatcttt tttgccttgg gtcaaacagc cagcctggca gcaatggacg caactgatga
     2701 aacgattcgg gatgaacgtt tgaatcgagc cttgggccat tatcgcctgg cagtgcagtc
     2761 ggcttcacct gaaacggata aggcggtttt atcgagagcg catgaatcga tggggcgaat
     2821 caatgcattt ctcgataata aagttgaagc cgcgcgagag tttgacgaag caataaagat
     2881 tggagaagtg agtggcggag cttacaagga agccgtggaa ggaaagaaga agctggccca
     2941 accggaatag ggaatagggg ccggattatt ggataattat gaattgatat ttgagaattt
     3001 ctatttccga tcgttgcggt gtttgaattc ctatcgtgag ttcttatcgg tcttgatcat
     3061 tggtcattgg tcattggtca tttggcattt ctgctggcag acaaagtcac acactgatgc
     3121 ttggaaaaat gataaatgac caatgaccaa gaccgataag aactcgcgat aggaattcaa
     3181 gcaccgcgac gatcggaaat agaaattctc aaatatcaaa tcataattat ccaatgattc
     3241 tgtccgactc cgacccctgg ccgcctaatc tactgctttc gcggcgttga ttctcccacc
     3301 cgaactgac
//
LOCUS       DASSFP010000375         5596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1885333, whole genome shotgun sequence.
ACCESSION   DASSFP010000375 DASSFP010000000
VERSION     DASSFP010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885333"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFH91_08410"
     CDS             <1..783
                     /locus_tag="VFH91_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6863057.1"
                     /translation="ATHTNCYLVHTSREILIFDPGSPYEDEQQSLAECVDMLIAEGRT
                     VREIIITHLHPDHVGGVNALRDHLNGNTVVAAHSETAQALPDIRVDRFIQDGETISLH
                     GEPAIHLQALHTPGHARGHLCFHDAEHNALITGDNIVGLGSVLIDPPQGDMATYLTSL
                     RRMRSLSNLKILFGGHGPAIATPYKKIDEYISHRLEREARILEAVQNGISIPAEIVRA
                     VYTDVSPKAYAMAERSVIAHLDKLLSEGRVTQLDQSRYSVIG"
     gene            890..2359
                     /locus_tag="VFH91_08415"
     CDS             890..2359
                     /locus_tag="VFH91_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863058.1"
                     /translation="MEYPRTLRELKNVRYETFSVKDELRLNLIKKLRTREKLFPGIVG
                     YDETVIPQLVNAILSRHNVILLGLRGQAKSRIIRQLTGLLDERIPIIAGSEVNDDPFR
                     PISAYGRQMVSHNGDLTPVEWIDRDSRFVEKLATPDVTVADIIGDVDPIKAAKGGHLL
                     SDELTIHYGLLPRANRGIFAINELPDLAGKIQVALFNIMQEGDVQIKGYPIRLPLDVM
                     LVFSANPEDYTARGKIISPLKDRIGSEIMTHYPKEVATSVQITKQEAWTDRFNDPNRI
                     VISEFIREVVDQIAFEARDDSHIDKHSGVSQRLPITVMESVVSNAERRALITGDEPIV
                     PRVADIYAAIPSITGKMELEYEGEQLGANRIAKDLIKRAAGEIFGGYFVGIDFSRAVK
                     WFDEGNKILLADTASADECRVLLEAVPDLIETASIPFEFDGNHDGQLVAACEFVLEGL
                     YAENKISRNEEGGYTAATKAKKDRRGMIYDDLTETGKYS"
     gene            2371..3678
                     /locus_tag="VFH91_08420"
     CDS             2371..3678
                     /locus_tag="VFH91_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6863059.1"
                     /translation="MKFTRYSKFKGFDVTSINLGQLMDGMADALLDSGYDDDYYWTRQ
                     RREADNSLDALRQALLQALMNQGLVDERQIAEMLAENEGKFKGSLLEELLNQLIERLV
                     EEGYLNLREVEPQKNEQRRGNGQGEISEPLQRDVRFEVTEKGLDFLGYKTLRNLLGSL
                     GKSASGRHDTVYLSTGVEAAAASKRYEFGDTINLDVNTTLLSAIQRDGLKVPINLEYQ
                     DLYVHQCDYQSSCATVVMLDCSHSMILYGEDRFTPAKKVALAMAHLIRTQYPGDSLKV
                     VLFHDSAEELPLGKLATTHVGPYHTNTAEGLRLSRRLLQAQRKEMKQIVMVTDGKPTA
                     CFLDAGSTASKGTTETTTRRLYKNSMGGDPFVMAETFKEVQACRKAGIMINTFMLASD
                     YYLVDFVKQMSAMTNGKAYFANPGNLSQFVLIDFLRRRTTRVR"
     gene            3794..>5596
                     /locus_tag="VFH91_08425"
     CDS             3794..>5596
                     /locus_tag="VFH91_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET6863060.1"
                     /translation="MSSPNPRPVRKFRPRQRVLLPYLILLLGLCFTLIVTHYFAKLAD
                     EQDRSRFQGTVQEINDRIKARIQTSITLLRAGTGLFAASDDVSAIEFERFVNQIELPK
                     HYEGVQGLGFSLRFSRHQLPQVVDDMRRAGYSTFKVWPDLPAREEYNAILFLQPAINR
                     NEVAIGFDMATEPVRRQAMDQARDSGNPTASGKVTLVQEPLGDTHQAGFLIYAPVYRK
                     DLPLNTEYERRQALLGFIYSPFRVDDFLSAVLAGTHYDVDFEVYDTSEIRSDTQMRGS
                     SSASATEGPPSHSVVQPLDVGNRTWTLVYKAKPSFEAGSNRGFLPYTFTTGILLSLLF
                     FAVTSAEVRARTRAELAAAELRESEATIRNTLEQREAAEEALREGEERYRVLVENAND
                     IVYMLRPDGRITSINTAAETITGYSTGELLSMNIKDLAAAGSQPVIEQMLDIKNDRGA
                     RTNFEITIQTKNGNPLTLEVSNRIISKGLVSIQGIARDISMRRRAEETLREADHRALS
                     EYERLLERISGLAVALGTGRELNTIFSALGEFTRVSVPCDGFLVSLYDPIRDVRTACY
                     AWADNKELDISELPPMPITTSGPNSRALRLAQVII"
BASE COUNT         1430 a         1389 c         1413 g         1364 t
ORIGIN      
        1 gcaacacata caaactgtta tctggtccac acgtcgcgcg agatcctgat tttcgatccc
       61 gggtcgcctt atgaggacga gcagcaatcc ctggctgaat gcgttgacat gctcatcgca
      121 gaaggtcgca ctgttcggga aatcatcatt actcatttac accccgacca cgttggtgga
      181 gtgaatgcgt tgcgcgatca cctgaacgga aatactgttg ttgccgctca ctctgaaact
      241 gcgcaggcct taccagatat tagagtcgat cgattcatcc aggacggtga gacgatcagt
      301 ctgcatggag agccggctat tcaccttcag gctttgcata ctcccggtca cgcccgcggt
      361 catctttgct tccacgacgc tgagcataac gcattgatta ccggagacaa tattgtggga
      421 cttggatccg ttcttatcga tccgccccag ggcgacatgg ccacatattt aacgtcactt
      481 agaagaatgc gttcgttatc aaacctgaag atcctttttg gcggtcatgg accggcgatc
      541 gcaacgcctt ataaaaaaat agatgagtac atctcgcacc gcctggagcg cgaagctcgc
      601 atacttgaag cagtgcagaa cggaatttcc attccagcgg aaattgtcag agctgtctat
      661 acagacgtct ctccgaaagc gtatgcgatg gcagagcggt cagtaattgc tcatctggat
      721 aaactgttgt ccgaaggccg cgtaacccaa ctcgatcaga gtaggtattc agtgatcggc
      781 taaacgtggc cgtaagtttt ccttcccccc tcttttcctt aatgaaatcg taattttgga
      841 gtcgcctgtg cttctgttgg ccgtttccgg ttaaaatcgt ttcatcatca tggaataccc
      901 aagaacgctt cgcgaactaa aaaacgtcag atatgaaacc ttctctgtta aagatgagtt
      961 gcgtctgaat ctaattaaaa aactgcgcac tcgtgaaaaa ttgtttcccg gcattgtcgg
     1021 atatgacgaa accgttatcc cacagttagt caacgcgatt ctatcgcgcc acaacgtgat
     1081 ccttctcgga ctacgaggtc aggccaagag tcgcatcatt cgtcagctaa ccggtctgct
     1141 ggacgaacgc atcccgatca ttgccggctc agaagttaac gacgatccgt ttaggcctat
     1201 ttccgcttat ggcaggcaga tggtgtctca taacggcgac ctgactccgg ttgaatggat
     1261 cgatcgagat tctcgtttcg tcgaaaaatt agcaacgccc gacgttaccg ttgcagacat
     1321 catcggagac gttgatccca tcaaagctgc aaagggcggc catctgctga gcgacgagct
     1381 gactatccac tacgggctgc tcccgcgagc taatcgcgga atctttgcca tcaacgaact
     1441 gccggacctg gcaggaaaga ttcaggttgc gctcttcaac ataatgcagg aaggcgatgt
     1501 ccagattaag ggatacccaa ttcgcctgcc tctcgatgtg atgttggttt tttctgcgaa
     1561 tcctgaagat tacacggctc gcgggaagat catttcgcca ctaaaggatc gcatcgggtc
     1621 agagattatg acccactatc caaaagaagt ggctacgtca gtccagatta cgaaacaaga
     1681 ggcctggacc gaccgcttta acgatccaaa ccggatagtt atctcagagt ttattcgtga
     1741 ggttgttgac cagatcgcct ttgaggcgcg cgatgatagt catatcgaca aacactcggg
     1801 cgtgtctcaa cgtttgccga ttacggtgat ggagtctgtt gtttcaaacg ctgaacgacg
     1861 cgctctgatc acaggcgatg aacccattgt gccacgagtc gccgacatct acgcggcaat
     1921 tccatcaatc acaggaaaga tggagctcga gtatgaaggt gaacaacttg gagcaaatcg
     1981 cattgctaaa gatctcatca aacgggctgc gggtgaaatc ttcggaggct acttcgtcgg
     2041 cattgatttt tctcgtgcgg tgaagtggtt cgatgaaggc aacaagatcc ttctcgccga
     2101 tactgcgtcg gccgacgagt gccgggtatt actggaggcg gtgcctgatt taatcgaaac
     2161 agcatcaatt ccttttgagt ttgatggcaa ccacgacgga caactggtag ccgcctgcga
     2221 attcgtactt gaaggtcttt atgctgaaaa caagattagt cggaacgagg aaggtggtta
     2281 cacggcggct acgaaagcga aaaaggatcg acggggaatg atctacgacg atctgactga
     2341 aaccggaaag tatagctaag cagtacactc atgaaattta caaggtattc caagttcaaa
     2401 gggttcgacg taacctcaat caatctcggg caattgatgg acggaatggc tgatgctctc
     2461 ctcgactccg gctacgatga cgattattac tggacacgcc aacgccgaga ggctgacaac
     2521 tcactcgacg ctttgcgaca agcgttgctc caggcgctca tgaatcaagg tcttgtagat
     2581 gagcgccaaa ttgctgaaat gctggccgaa aacgaaggca aattcaaagg atcgttgctc
     2641 gaggagttgc taaatcaatt aatcgagagg ctggttgaag aaggctatct caatcttcgg
     2701 gaagttgaac ctcaaaaaaa tgagcaacgc cgtggaaatg gccaggggga gatttcggag
     2761 cccttgcaga gagacgttcg ttttgaagtg actgaaaaag gtctggactt tttgggttac
     2821 aaaaccttga gaaatttgct tggcagttta ggtaagagtg cttccggcag acatgacacc
     2881 gtataccttt cgactggagt tgaagctgct gctgccagca agagatacga atttggtgac
     2941 acaatcaatc tcgacgtgaa cacgacatta ctatccgcca ttcagcgaga tggattgaag
     3001 gtgcctatta atcttgagta tcaggatctt tatgttcatc agtgcgatta ccaatcgtcg
     3061 tgcgcgactg tcgtaatgct tgattgttcg cactcaatga tcttgtacgg cgaagatcga
     3121 tttacacccg ccaagaaagt tgccctggcg atggcccatc tgatccgaac tcaatacccg
     3181 ggcgactcgc tgaaagtcgt gctatttcat gattcagcag aggaactacc ccttggaaaa
     3241 cttgctacta cgcatgttgg tccctaccac accaacaccg ctgaggggct acgcttatct
     3301 cgtcgactgc ttcaagcaca gcgtaaagag atgaagcaaa tcgtaatggt aactgacggc
     3361 aaaccgactg cctgcttcct tgatgccgga tcgactgctt ctaagggtac gactgagaca
     3421 acaaccagac gcctttataa aaattcaatg ggaggcgatc cgtttgtgat ggctgaaacc
     3481 tttaaggagg ttcaggcgtg tcgcaaggct ggcataatga tcaatacttt catgcttgcg
     3541 agcgactatt accttgttga cttcgtcaaa cagatgtccg caatgacaaa tggcaaagct
     3601 tactttgcaa accccggcaa cctatcgcag tttgtcttga tagatttcct gcggcgacgc
     3661 accactcgcg ttaggtgaat cgccgacagt cagagaattc tctggacatt tagtgacttt
     3721 tacaacgaac tccaggtccc gctcacagag gggataattt gttcgttgat tactgacacg
     3781 ttccctgaac tgtatgtcca gtcccaaccc acgcccggtt cgcaaattcc gtccacgcca
     3841 acgcgttctt ctgccttatt tgattttgct tctcggtctt tgcttcacat taatagttac
     3901 gcactatttc gcaaagctcg cagatgaaca ggaccggagt cgcttccagg gcacggtgca
     3961 ggagatcaac gaccgcatta aagctcgcat tcaaacttca attactttgc ttcgggccgg
     4021 tacaggactc ttcgctgcga gcgatgacgt cagcgcgatc gagtttgaac gcttcgtaaa
     4081 tcagatcgag ttgccgaaac attatgaagg tgtgcagggt ttaggctttt cgcttcgttt
     4141 ttcgcggcat cagttgccac aagtcgtcga cgatatgcgc agggccggct actcaacctt
     4201 caaggtatgg ccggatcttc ctgcgcgcga agaatataat gcgatcctat ttttgcagcc
     4261 tgcgatcaat cgcaatgagg tcgccatcgg atttgatatg gcgacggaac cggtccggcg
     4321 gcaggctatg gaccaggccc gtgactccgg aaatccaacg gcatcaggaa aggttacgtt
     4381 ggtccaggag ccgttgggcg atacacatca ggccggcttt ctcatttatg cgccggttta
     4441 tcgaaaagac ttacccctta ataccgagta cgagcgccgg caagcgctgt tggggtttat
     4501 ctacagtccg ttccgcgtcg atgacttttt gagtgctgtt ctggccggca ctcactacga
     4561 tgttgatttt gaggtatacg acacaagtga gatccgaagt gatactcaaa tgcgtggttc
     4621 gagttctgcg agcgcgaccg agggcccacc atcgcattcg gtggtccaac cactcgatgt
     4681 aggtaatcgt acctggactc tggtctataa agctaagcct tcatttgaag caggttcaaa
     4741 tcgcgggttc ctaccttata catttactac cggaatcctt ctaagtctgc tcttctttgc
     4801 tgtcactagc gccgaagtac gcgcaagaac tcgtgccgag ttggcggctg cagaattacg
     4861 agaatctgag gcaacaattc gaaacactct cgaacaacga gaagctgctg aagaggcgct
     4921 tcgtgaaggt gaagaacgat atcgagtctt ggttgaaaat gctaacgata ttgtttacat
     4981 gctgaggccg gacggcagga taacctcaat caacacagcg gcagaaacga ttacgggcta
     5041 ctcgaccggc gagttactct cgatgaatat caaggacctg gcggcggccg gatcccagcc
     5101 ggtgatcgaa cagatgctgg atataaaaaa cgatcgcggg gcccggacga acttcgagat
     5161 aacaattcag acaaaaaatg gtaatccact aacactcgaa gtcagtaatc ggattatttc
     5221 taaagggctc gtctcgattc aaggaatcgc tcgagatatc agcatgagac gacgagccga
     5281 ggaaacactg cgcgaagcgg atcatcgagc tctttcggag tacgagcgat tactggaacg
     5341 aatttcaggc cttgcggtgg ctttaggcac tggtagagag ctaaatacga ttttcagcgc
     5401 gctgggtgaa ttcacccggg tgtctgttcc ctgcgacggc ttcctcgttt ccctttacga
     5461 tccgattcga gatgtgcgaa ctgcttgtta tgcctgggcc gacaataaag aattagatat
     5521 ctcggaactc ccaccgatgc caatcacgac ttcgggccca aacagccgag cgttgcgcct
     5581 ggcccaggta atcatc
//
LOCUS       DASSFP010000376         3866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1738213, whole genome shotgun sequence.
ACCESSION   DASSFP010000376 DASSFP010000000
VERSION     DASSFP010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738213"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFH91_08430"
     CDS             complement(<1..1326)
                     /locus_tag="VFH91_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6863061.1"
                     /translation="MRLTFIQKKRRSSRFSTAAIFFLTFSLVLSGCFNVQEPSTYYGR
                     VVVPRSQEFRWSDGGLPQTFDPAFAAAAPDTDAVRALFEGLTDYDPKTLSPIPAVATR
                     WESTGDGRIWTFYLRNDARWSNGDKVTAQDFVRSWKRTLEIGPLAPHNELLANIQGAV
                     GNRILNHKVETPKATGEFGAEALGDNVLRVKLERSDSSFPALVAHPVFRPVKFSDADP
                     TRRLEAPDLISNGAFQLSNTSNARVSLEPSKMYWDGEAVTLQRVTFVNTTNAEDALSA
                     YRAGDVDAVTNAAFEPLALKLLAPYRDFRRSTFGAVTYYNFNVAHEPFDDIRVREALA
                     ISIDRDRVSRDDLDGATEPAGKFFPEPSADDQPVVGKTALIEQDVNKAKQLLAEAGFP
                     NGNGFPTVRLLINRNEQQRIVAQAIAEMWKNVLNIQTEIVVKNWDEYEAA"
     gene            complement(1323..1931)
                     /gene="coaE"
                     /locus_tag="VFH91_08435"
     CDS             complement(1323..1931)
                     /gene="coaE"
                     /locus_tag="VFH91_08435"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET6863062.1"
                     /translation="MLRIGLTGSIGVGKSFVASVFVELGCHLIDADQTAREVVLPGSE
                     GLRAVTDHFGQEVLLEDGSLNRKALGAIVFADEQQRLVLNSILHPFIIARQDQLLREW
                     EEKDPTGIGIVDAALMIESGGYARFDKLIVVHCRPEVQLERLMLRDGLTRDQAQRRID
                     SQMSQEEKQRFADYLIDTSDGFEPTRLQTKQVYEALLQLTRR"
     gene            complement(2143..3093)
                     /locus_tag="VFH91_08440"
     CDS             complement(2143..3093)
                     /locus_tag="VFH91_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET6863063.1"
                     /translation="MITQNALKQHAQLLDGSIVAERIKQDVKSEIELLSQSKGVTPCL
                     AAIRVGDDPASAVYVRNKIRASEEVGIRSEHHALPATTTTEELLDLINSLNSRDDVDG
                     ILAQLPLAAGIDESLIIEAIDPAKDVDGFHPINIGRLAMGRPALVPCTPAGIIELLDY
                     YEIQLKGVNACVVGRSQIVGRPMAQLLLQRDATVTICHSRTKDLPLVTSQADLLIVAI
                     GRAGMVRRNYIKPEATVIDVGMNKITDADDVAALFGEAAQKRLEVIAKRGYTLVGDVN
                     PAEAGEVAGRLTPVPGGVGLLTVAMLMKNTLKAAKLRRGI"
     gene            3336..>3866
                     /locus_tag="VFH91_08445"
     CDS             3336..>3866
                     /locus_tag="VFH91_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863064.1"
                     /translation="MTVKRLVALLTLNILLQSALVYGQSSDDLSKRIRTAVENENWQT
                     ATTELEKLRTADPAFFQSRDYDYLAARISERSGDTAAASKSYQTIVSGTSILSEHALW
                     RLARLARSTGDLPLERERLRRLIPVASGSLLYEPATLRLAESFFESGDYQAAAVAASS
                     LTNVKNSSLAREAAALM"
BASE COUNT          944 a         1017 c          944 g          961 t
ORIGIN      
        1 agccgcttcg tactcatccc aatttttgac gacgatttcc gtctgaatgt taaggacatt
       61 cttccacatc tccgcaatgg cctgggccac aatacgttgc tgctcatttc tattaatgag
      121 cagtcttacg gtcggaaatc cattgccatt cggaaaaccc gcctctgcca gtaattgctt
      181 tgctttatta acgtcttgct caatcagtgc ggtctttcca acgacgggtt gatcatcagc
      241 cgacggttcg gggaagaatt tacctgcggg ctcagtagct ccatcaagat catcacgact
      301 tacgcggtca cggtcgatcg atatggcgag tgcttcccta acccgaatat cgtcgaaagg
      361 ctcatgtgcc acgttaaagt tgtaataagt gacggcgcca aaagtcgagc gacggaaatc
      421 cctgtacggg gcaagaagtt tcagagcgag aggttcaaac gctgcgttag tcacggcatc
      481 gacatcgccc gcgcggtagg cagacagagc gtcttcagca ttggtagtgt taacgaaggt
      541 cacgcgttgc agtgttaccg cttcgccgtc ccaatacatt ttcgatggtt ccagcgaaac
      601 ccgcgcgttg gatgtgtttg atagttggaa ggcgccattc gaaatcagat caggcgcctc
      661 gaggcgtcga gtcggatccg catcagaaaa cttgactgga cggaaaaccg gatgagctac
      721 cagagctgga aagctgctat ctgagcgctc gagttttacg cgcagtacat tatcgcccag
      781 cgcttcggcg ccaaattcac ctgtcgcttt aggagtctca actttatgat tcaaaatcct
      841 gttgccgacg gctccctgta tatttgccag caactcgtta tgcggagcca gtggtcctat
      901 ttcaagagta cgcttccatg accgaacaaa gtcctgagca gtaaccttat cgccgttgga
      961 ccatcgagca tcattgcgta aataaaaagt ccatattcgt ccgtcgccag tcgattccca
     1021 acgagttgcg actgcgggta tgggggaaag tgtcttggga tcgtaatctg tcaggccttc
     1081 aaatagtgct cttacggcgt cggtatcggg cgcagcggcg gcgaatgcgg gatcgaatgt
     1141 ctgaggcaag cctccgtcac tccaacggaa ctcctgagat cgtggaacga cgactctacc
     1201 gtaataggta gagggctcct ggacgttaaa gcagcccgat aggaccagac tgaacgtaag
     1261 aaagaatatg gctgccgtag aaaacctgga gcttcttctc ttcttttgaa taaaggtaag
     1321 tctcatctgc gagttaactg gagaagtgct tcataaacct gtttggtttg tagtcgcgtt
     1381 ggttcaaaac cgtcagaagt gtctatcaaa taatcagcaa atcgctgttt ctcttcctgc
     1441 gacatttgtg agtcgatcct gcgttgggcc tggtcgcgag ttaatccgtc gcggagcatc
     1501 agcctttcga gctgcacttc agggcggcaa tgaaccacga taagcttatc gaaacgagca
     1561 taacctccgg attcaatcat caacgcggcg tcaactatac caattccagt tggatccttt
     1621 tcttcccatt cacgcaacag ttgatcctgg cgcgcaataa taaatggatg aagtatagag
     1681 tttagaacca gacgctgttg ctcatccgca aaaacaattg ctcccagagc tttccgattt
     1741 agcgaaccgt cctcgagcaa tacctcctgg ccaaagtgat ctgtaactgc ccttaaaccc
     1801 tcggagccag gcaaaacaac ttcacgggcc gtttgatctg cgtcgatcaa atgacatccg
     1861 agttcgacaa acacggaagc aacaaacgac ttgccgactc caatggatcc ggttagcccg
     1921 atacgtaaca tagttgtcag ttgtcagttg tcagtcgttc acctgttttt tgtgctttgt
     1981 acttcgccct ttgtgcttag cccttcgtgc ttagcccttc gtgctttgta ttttggattt
     2041 cgcactttca cctctgcagc cacgacaccg ttagaatctc aaagcacaaa ggacaaaact
     2101 ccggggtgct acggacaacg gccacgggcc acgcaccacc ctttaaattc ctcggcgaag
     2161 ttttgcggct ttgagtgtgt tcttcatcag cattgccacc gttaagagtc ctacgccacc
     2221 gggcacaggc gtcaggcgtc cggctacttc gccagcctca gcaggattga cgtctccaac
     2281 cagtgtgtag ccgcgcttgg caatcacctc gagtcgcttc tgcgccgcct ctccgaacag
     2341 ggcggcgacg tcgtcagcgt ctgtaatctt gttcataccg acgtcgatca ccgtagcttc
     2401 aggctttatg tagttgcggc ggaccatacc agcgcgccca atcgcaacta ttaacaggtc
     2461 tgcctgacta gtgacgagcg gcaagtcctt agtgcgagaa tggcagatag tgaccgtagc
     2521 atctcgttgc aagagcaatt gggccattgg acgaccaaca atttgactgc gtccgacaac
     2581 gcaagcgttc acgcccttaa gctgaatctc gtagtagtcg agtaattcaa ttattcccgc
     2641 gggcgtacaa ggcacgagcg caggtcttcc cattgccaat cgcccgatgt taattggatg
     2701 gaaaccgtct acgtctttgg caggatcaat cgcctcaata attaagctct catcgatgcc
     2761 ggccgctaat ggcagctggg ccagaatgcc atctacatca tctctggaat tcaatgagtt
     2821 gatcagatcc aaaagctctt cagtggtagt agtcgcgggc aaggcgtgat gttccgaccg
     2881 tatcccaact tcttcagaag cccgaatttt atttcgtaca tacaccgcgg acgccggatc
     2941 atcaccaacg cgaatggctg caagacaggg agtcacccct ttactttgag acaataactc
     3001 gatctcggat ttgacatcct gcttaatgcg ttccgcaact attgagccat cgagtaattg
     3061 agcgtgttgc tttaaagcgt tttgcgtaat catgaataat tgaataactc gccttgaaga
     3121 agtttgaaat cgttggacaa aggttacaac gactccatgg aaatgaatag cgaccatcga
     3181 aaaaagccag cgtcctaaac ctgcacgcgc gctatccagt gtgaaatttt atcaaggctt
     3241 gagagttgag aagctcctcg cggagtgggt tgacttcttc aagcgaatgg ttcgagtaac
     3301 tggataatgt gagtgtgggt gttattatcc gagacatgac agtaaaacgg ctcgttgcgc
     3361 ttctaacgct taatatccta ttacagtctg cccttgtcta cggccaatct tcggatgact
     3421 tgtcaaagcg cattcgtact gccgttgaaa atgaaaattg gcaaacagcg acaacggaat
     3481 tagaaaaact tcggactgca gatcccgcat ttttccaaag ccgtgattac gattatctag
     3541 ctgctcgtat atccgaacgc tcgggagaca cagcagccgc gtccaaaagc taccagacaa
     3601 tcgtcagcgg cacttcaatc ctttcggaac acgctctttg gagattagcc cgcctggcac
     3661 ggtcaaccgg cgatctccct ctcgaacgcg aacggcttcg gcgactaatt cccgtggcat
     3721 ccggaagtct gctttacgaa cctgcgacgc tcaggctggc cgagagcttt tttgaaagtg
     3781 gtgattatca agccgctgcc gttgccgcca gctctctaac taatgtcaaa aatagttccc
     3841 tcgcgcgtga ggcggcggct ttgatg
//
LOCUS       DASSFP010000377         7508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1441204, whole genome shotgun sequence.
ACCESSION   DASSFP010000377 DASSFP010000000
VERSION     DASSFP010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441204"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFH91_08450"
     CDS             <1..440
                     /locus_tag="VFH91_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6863065.1"
                     /translation="PAGHASCFNCHWKEQQPVAENCAGCHKLTKPYSNVEVPSRISMK
                     FIHDGGGEKKNHVAECTTCHINITKAVSLRGLKPDVPITACTECHNKEGLRQDVSKEL
                     MAIDKDKNFTCTYCHTSDVGKLDPPASHYLIAERPPLKRKDVK"
     gene            900..1130
                     /locus_tag="VFH91_08455"
     CDS             900..1130
                     /locus_tag="VFH91_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863066.1"
                     /translation="MLRSQFRRNLRLITAVIVVTRQPANHRLGEKSHRAAFVMIRNRI
                     GVRALQRVLFGSRSAMGTMGPESDSPVRTAIS"
     gene            1591..2169
                     /locus_tag="VFH91_08460"
     CDS             1591..2169
                     /locus_tag="VFH91_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6863067.1"
                     /translation="MKITLILLLVSGCIGLVVFGGQPGPAHVAIVVQEKESKTQPKDI
                     VLSKDSQSDKYGEVPFSHENHATKKYSPDGQAMLTCVECHHTDQPAANLKAPLKTSER
                     SVTLTAEVLQAADSKAVKSCRACHLQAGDDSAPLPSVQYPDKPAPTKLTNEVAYHLNC
                     NLCHDKALAARPALKGKVPGSNDCLPCHKPAG"
     gene            complement(2151..3581)
                     /locus_tag="VFH91_08465"
     CDS             complement(2151..3581)
                     /locus_tag="VFH91_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6863068.1"
                     /translation="MAEKNQYVIIRDDLPIDPLQVTSEGLLIGRLLECEIVLNHPAVS
                     RVQAGIKQVDGNFFLFPLRPTNPVVLNGKPVQENEALAAGDLLLVGPFQLAVDSADES
                     LVIRVSLEIAVNPSDMENSKPGFSTEKLVTPTDGKAKKARAAPIPGTKALDIFWDKRI
                     REAGKVVRPSPLFPKSQRRSGKAQSNWTPTSDLKTRWPISFFVWSVIVVGTLSAGAAY
                     WYSRAYSPGPISNAHSLSQMSLAPAIATKANAGSCTNCHGLKASMDERCSSCHQTPAF
                     AASVIPPHSAAGIGCVNCHAEHRGSGFSATEGALASCTECHNNDNSRTFNGKRVGTPH
                     GGTFGYPVVNGIWSAKSINEEEWDLRKLPIVRLPTDTEDKWRSKQFHALHSQRVKLVA
                     GLTGNAQGQLSCSSCHKSFDPIDRETPRTTCGLCHNGKVDANTNKTLIVTNKPNCTSC
                     HVQHVHDTRRWGASMLAQPSPQPAGL"
     gene            complement(3604..6081)
                     /locus_tag="VFH91_08470"
     CDS             complement(3604..6081)
                     /locus_tag="VFH91_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6863069.1"
                     /translation="MPREIADRTRVVDAVKNLSLTSELFEEQNGKQKYAADLEVIVQG
                     REYSNGKQVGPYVRLLQYDANEVIMHQGEWGGNTFYIAVDGTLDVSVEDGDKAKQIGQ
                     LQPGTLFGEMGLLAGIERNATVSATGSSGATVLEVTRPALRLLRKLPKFGQILDETYR
                     GHGFGRVLEDLALASNGGLSPKLVEKLGQNARYMVYGKHHVLCKEGEPIDRLILIKSG
                     WVRRSTGIRFYAASPEVVRGMGETIGVDFIGAGNLLGLEGLSEESNWLYGASLMSRTE
                     LLEVPLAPFETDLQLRNELAAALASFSDADHELPPTAETVPDINALASAEKEIATGVI
                     DGANVLVMDMDLCVRCGNCSLACHKVHGHSRLLRRGIHIERPIRLGAKRLQHALVPQV
                     CMHCKDPECLTGCPTGAIFRDPQGQVDIDPHTCIGCFDCATQCPYDAISMVPKKSEPL
                     PQTLGDRIARLFSLRTVVTATPPVAADEDVVAIKCNLCENTPLNPTGSKRKAYSCEEN
                     CPTGALVRVNPLEYFSEIGPTQGLIFRDETHAVGRNIHKSDPLARTWHVSGAFVTVVI
                     AVWFIWLLQIYGFDRPLNGGWLTMRWLTGIVGLVGVAGVMTYPFRKEVYRRRAGPLRY
                     WMLAHIYLGTIAGVVLLVHAGTHTGGLLTTVLYVSFDVVLLSGVVGILTYLIAPRIMT
                     RIEGEPLLVEDLIGRREELLIEKDDILKQSEGWLKEEIEQKIYSQFLSRSYLTRQLIR
                     HESLKALLAEARADYKDRITRLATDDERKLLLSAVETAVTLTRIDALILLHRSLRFWI
                     PIHVISTALMLALMLVHVFQVVFFKVR"
     gene            6365..7507
                     /locus_tag="VFH91_08475"
     CDS             6365..7507
                     /locus_tag="VFH91_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6863070.1"
                     /translation="MHDSLDSLPLAQQIGQLFFIGLPGTEIDNETRELIQEVNPGGVI
                     IFGRNVAAPQQLRSLLDGVRELVPTQPLFGIDQEGGLVDRLRKIFTPMPSARTIRQHG
                     DLASARALGRITGEVLRLLGFSINFAPVMSIMTDDRDLLSNGLYSRSFGRSPGEVLGY
                     TTVYMRGLQGTGCLGCLKHFPGIGSGEVDSHEEMPVVRLSHDDLIAQDLAPYIELFQR
                     KDDRVRVVMVSHGGFPNVDIREETTGGLIEPASLNYNIVTKLLRDELGYKHLVVTDDL
                     EMGAIGRHCEIEDAVVRAALAGEDMLLICASPDKIRRGYRSMLAAAKDGRLPEKRLRE
                     SLRRISNTKGIAGPPLDLDMERFKFLAGEVTKLNDKLNYTYGGTVK"
BASE COUNT         1814 a         1980 c         1932 g         1782 t
ORIGIN      
        1 cgccggcagg tcacgcatcg tgttttaact gccattggaa agaacagcag ccggtagcag
       61 aaaactgcgc cgggtgtcac aagctgacca agccctattc gaatgtcgag gtaccctcga
      121 gaatttcaat gaagttcatt catgacggag gtggtgagaa gaagaaccat gtggccgaat
      181 gcaccacttg ccacatcaac attacaaagg cggtatcgct ccgcggacta aagcccgatg
      241 tgccaattac tgcgtgcacc gaatgtcaca acaaagaggg tctgcgccag gatgtcagta
      301 aagagttgat ggcaatcgac aaggataaga attttacgtg tacctactgt cacacttccg
      361 atgttggaaa actggaccca ccagccagcc attacctgat cgctgagcgg cctcctctaa
      421 aacgaaaaga cgtcaaatga acgtgcctcg acaaagcaat tttcggaatt atctgggtat
      481 cctgctttca ggcgctcttt tgtcatcttt tttcgggatc gctttgttta gttcggaagt
      541 tacgatcagc acattggctc agggaccgga cgtcgattac tcgaaattcc tgcataccag
      601 ctcgcggcat tcgtccatcg cttgcaatgc gtgtcacgaa cgtacggata actcagcgac
      661 gccccgcttt ccaggtcaca acgcctgtac cacctgtcat attggacaat tcacgacgcc
      721 ggcgatccca atgtgcaaaa tctgtcatac ggaaacgagc ggcgcgcggc cccccctcaa
      781 aaactttccg acggacttta aagagagctt taacgttaag ttcgatcact cgcaacacat
      841 gaacggcgcg gcccggcctc aaaatggatg caacggttgt catactgcca tgaaccggaa
      901 tgttgcgttc tcaattccgg cggaacttac ggctcataac ggctgttata gttgtcacac
      961 gccagccagc aaatcatcgg ctgggagaga aatcgcatcg tgcggcgttt gtcatgatca
     1021 gaaatcgtat cggcgtacga gctctgcagc gcgtgctttt cggctctcgt tcagccatgg
     1081 ggaccatggg accagagagc gactcgcctg tgcggactgc cataagttga ctgcgggtgc
     1141 gccccaaacg aagcaggtct cttcccccgc gtccgctcag cattttgcag gaaatagcca
     1201 gagttgctcg acctgtcaca acggccggca aacgtttggt ggtgacctcg gattcaaaga
     1261 ttgtaaacgc tgtcatactg ggccaacgtt caggatggga ggataaatgg gggatggaac
     1321 cagagtaagt gtgaattgat agttgatatt tgagaattga tcaggtctta ggtctttgat
     1381 ctttgtggtt tgtgagcttc gaatttgagt ttactgtcga tcgctcgatc acagatctca
     1441 aagttacaat ctgaaagggg cgtagtccga aggccaaacg ccaaaggcca aagctcaaag
     1501 gcccaaggcc gaattaaaaa ttatcaattg ataattcacc aatggtaaga ttccttccca
     1561 gcgctcaatc ttgttcttag aggacaatcc atgaaaataa ctctcattct attgcttgtc
     1621 tccgggtgca tcgggctcgt cgtattcggc ggtcagcccg ggccggccca tgtcgcaatc
     1681 gtggtccagg aaaaagagag caaaacccaa cctaaggata tagttctctc aaaggacagt
     1741 cagtcggaca agtatggcga agttccattt agtcatgaga accacgctac gaagaagtac
     1801 agccccgacg ggcaagcgat gctcacctgt gtcgaatgcc atcacacaga tcaacccgcc
     1861 gcgaatctga aggcgccact gaaaacttca gaacggtcag taacgttgac agcggaggtt
     1921 ctacaggctg ccgattccaa agctgtgaag agttgccgtg cgtgtcatct tcaagccggc
     1981 gacgacagcg ctccgctgcc ttcggtgcaa tacccggata agcccgctcc caccaaatta
     2041 accaacgagg ttgcttatca tctcaactgt aacttgtgtc acgacaaggc gctcgccgct
     2101 cgacccgcgc taaaaggcaa ggttcccggt tcaaacgact gtcttccctg tcataaacct
     2161 gctggttgag gtgaaggctg ggccaacatt gaggctcccc aacgacgtgt atcgtggacg
     2221 tgttggacgt gacacgacgt gcagttaggt ttattggtga caattaaggt cttgttggtg
     2281 tttgcgtcta ccttcccgtt atgacaaagg ccgcaagtag tccgcggcgt ttcccgatct
     2341 atcggatcaa acgatttgtg acaactcgaa catgagagct ggccttgcgc gtttccggtt
     2401 aagccagcga ccagttttac tcgttgagaa tggagtgcat gaaactgctt acttcgccat
     2461 ttgtcctcgg tatccgttgg aagacgcaca attggtagct ttcgcagatc ccactcttct
     2521 tcgttgatcg acttcgcgct ccaaatacca ttcactactg gatagccaaa cgtccctccg
     2581 tgcggcgttc ccactctctt gccgttaaag gttcgtgagt tatcgttgtt gtgacattct
     2641 gtacaagacg cgagcgcacc ttcggtcgcg ctgaaacctg agccccggtg ctcagcatga
     2701 caattgacgc agcctatgcc agcggcagag tgcggtggga taacagatgc agcaaaggca
     2761 ggagtctgat gacaactact gcatcgttca tccatgctgg ctttgagtcc atggcaattc
     2821 gtgcaggatc ccgcattcgc ttttgttgct attgccggcg ccaatgacat ttgtgataac
     2881 gaatgtgcgt tcgatatcgg tccgggagaa taggccctgc tataccagta cgccgcgcca
     2941 gctgagagtg tacccacaac gattacggac cacacaaaga atgagatggg ccagcgcgtc
     3001 ttaagatctg aggttggggt ccaatttgat tgggccttgc ctgaacgtcg ctgactcttc
     3061 gggaagagag gggaaggccg cacgactttg ccagcttcac ggatacgttt gtcccagaag
     3121 atatcgaggg cctttgtccc cggaatcggc gccgcacgag ctttcttcgc ctttccatcc
     3181 gttggtgtaa ccaacttctc cgtgctgaag cctggtttac tgttttccat gtcagaggga
     3241 tttaccgcaa tttccagtga gacccgaatg acgagcgatt cgtccgcgct atctacagca
     3301 agctggaatg gtccaactag caacaggtcc ccagcggcca gcgcttcatt ctcttggacg
     3361 ggctttccgt ttaggactac aggattggtc ggtcgcagtg gaaatagaaa gaaattcccg
     3421 tctacttgtt tgattccagc ctgcacgcgc gacacagccg gatgattcaa gacaatttcg
     3481 cattccaaca gtctgccgat gagcagacct tcggatgtga cctgaagtgg gtcaatcgga
     3541 agatcgtcgc gaatgattac gtactggttc ttctcagcca tcgatccggt ctgcgccaga
     3601 gagttacctg accttgaaaa atacaacctg gaaaacatgg accaacatta aggcaagcat
     3661 tagtgccgtt gagattacgt gaattggtat ccagaacctg agtgagcgat gaagcaatat
     3721 taaggcgtca atacgcgtta gagttactgc cgtctcaacc gctgagagca agagtttacg
     3781 ctcgtcatcc gtcgccaatc gcgtaattcg atctttgtaa tccgcccttg cttccgcgag
     3841 aagcgccttc agtgactcat gccgaatgag ctgccgagtg agataggatc gagagaggaa
     3901 ctgagaataa atcttttgct caatctcctc tttaagccaa ccttcgcttt gcttcaaaat
     3961 gtcatctttt tcgatcagca gctcttcacg ccggcctatt aagtcttcaa cgagaagagg
     4021 ctcgccttca attcgggtca ttattcgagg cgcaataaga tacgtaagaa tccccactac
     4081 accagagaga aggaccacat cgaaagatac atagagaacg gttgtcagga gaccacccgt
     4141 gtgagtgccg gcatgcacca ggagcactac cccagcgatg gtaccgagat agatgtgagc
     4201 cagcatccag tagcggagag ggcccgctcg gcgacgatag acctctttcc tgaacgggta
     4261 cgtcattact cccgcgacac caaccagccc gacgatgcca gttagccagc gcatggtgag
     4321 ccagccgccg tttagtggac gatcgaagcc gtagatttgg agtaaccaga tgaaccagac
     4381 agcgattact acagtcacga aggcgccgct gacatgccac gttcgggcta atggatcgct
     4441 tttgtgaata ttgcgtccta cggcatgagt ctcatcgcga aagataagac cctgggttgg
     4501 accaatttca ctgaaatact cgagcgggtt aacgcgcacc agggcgccgg tgggacagtt
     4561 ctcttcacat gaatatgctt tgcgctttga ccctgttggg ttaagaggtg tgttctcgca
     4621 caagttacat tttatggcga caacatcttc atcggcggcg acaggtggtg tagccgtcac
     4681 aactgttcgt aaagaaaaaa gtcgtgcgat cctgtcgccc agtgtctggg gaagtggctc
     4741 actcttttta ggaaccatcg agatcgcgtc gtagggacat tgcgtcgcac agtcgaaaca
     4801 gccgatgcag gtgtgcgggt cgatatcgac ctgaccttgt ggatcacgaa agatcgcacc
     4861 ggtcggacat ccagttaaac actcgggatc cttgcagtgc atgcatactt gcggcaccag
     4921 tgcgtgttgc aaccttttcg ccccgagcct gatcgggcgc tcgatgtgta ttcctcgacg
     4981 gagcaaccgg gaatgcccgt gcaccttgtg acacgccagt gaacagttgc cacagcgaac
     5041 gcagaggtcc atgtccatga caaggacgtt ggccccatcg atcaccccgg tagcgatctc
     5101 tttctctgcc gatgcgaggg cattaatgtc aggcactgtt tccgcagtag gtgggagctc
     5161 gtggtccgca tcagaaaaac tcgccaacgc cgccgccagt tcattccgca gttgtaagtc
     5221 tgtttcaaac ggagcaagcg ggacttcaag gagttcagtg cgagacataa ggctcgcgcc
     5281 gtacagccag ttggattctt cgcttagtcc ttccagaccg agtagattcc ctgccccgat
     5341 gaagtcgacg ccgattgttt ctcccattcc cctaacgacc tcgggtgagg ctgcatagaa
     5401 tcgaattccg gttgaacgtc tgacccagcc acttttgatc agaatcaaac gatcgattgg
     5461 ttcgccttct ttacagagca catggtgttt gccatagacc atgtaacgag cattctgccc
     5521 gagtttttca acgagtttgg gacttagacc accgttcgat gccagagcga gatcctcgag
     5581 cactcgaccg aaaccgtggc cacgataggt ttcatcaaga atctgtccga atttaggcag
     5641 tttacgaagc agacgaagcg ctggcctggt cacttcgaga acagtagcgc cggaactccc
     5701 agttgcagat actgttgcat tccgctcgat tcctgcgagc agccccattt caccaaagag
     5761 tgtgccgggt tgaagttggc caatttgttt tgccttatca ccatcttcaa cagagacatc
     5821 cagagtgccg tcgacggcta tgtagaaagt gtttccgccc cactcaccct gatgcatgat
     5881 cacttcattc gcatcgtatt ggagcagccg aacgtatggt ccaacttgtt taccattgct
     5941 gtactcacga ccctgaacga tcacttcgag gtcggccgca tatttttgtt ttccgttttg
     6001 ttcttcgaaa agctcagagg tgagtgagag gttcttgacg gcgtcgacta cacgagtccg
     6061 atccgcaatt tcgcgtggca tatagtcgtt tatactactt acggcgtcaa agttaaatgt
     6121 ttaaattttc ctgactttgg agtgcggcgg tccggcgccg cttttaacct gaaactaccc
     6181 gcgacccaca agttggaaaa cgccacctgc cgggggttct tttttccgac acattgtgca
     6241 aaaactcacc ctcgacgtgc taaactctcg cgacattgcg tactcagtgc ggcgcacaaa
     6301 gtacgaagca caaagtgcaa agtacaaata caaaccacaa attgtcttaa ttctgaaatc
     6361 ataaatgcac gattcacttg attcacttcc actcgcacaa caaatcggcc aactcttctt
     6421 tatcggactt cccggaacag aaatcgataa tgagacacgc gaactaatcc aggaggttaa
     6481 tcctggtggc gtcatcattt tcggtcgcaa cgttgcggcg ccacagcagc ttcgctcttt
     6541 gctggacggc gtgcgagagc tggttcccac tcaacctctg ttcggaatcg atcaagaggg
     6601 cggtctggtc gatcggctga gaaaaatttt tacgccgatg ccgtctgcac gaactatccg
     6661 tcaacatggc gacctggctt cggcgcgcgc gcttggacgg ataaccggtg aagtgctccg
     6721 tttgcttggc tttagtatca atttcgcgcc ggtcatgtcg attatgactg acgatcgcga
     6781 ccttctttca aatgggctct attcccgttc gttcggtcgt tcaccgggcg aagtacttgg
     6841 ctacacaacg gtctacatgc gcggacttca gggaaccggc tgcctgggtt gtctgaagca
     6901 ctttcccgga ataggttcgg gtgaagttga ctcgcacgaa gaaatgccgg tggtccgtct
     6961 ttcacacgac gacctcatag ctcaagatct tgctccctac attgaactat tccaacgaaa
     7021 agacgatcgt gtgcgggttg tgatggtgag ccatggcgga tttcccaatg tagatattcg
     7081 ggaagagaca acaggtggct taatcgagcc agcttcgctt aactacaaca tcgttaccaa
     7141 acttctacga gatgaattgg gctacaaaca tctggttgtc acggatgatc tcgagatggg
     7201 cgccattggt cggcactgcg agatcgaaga cgcagtggtg cgtgcggctc tggcgggaga
     7261 agacatgctg ttgatctgcg caagcccgga caagattcgc cgtggttatc gttccatgct
     7321 tgcggccgct aaggatgggc gcctgcctga aaagaggctg cgcgaatcct tgagacgcat
     7381 cagcaacact aaaggtatcg ccggcccgcc actcgacctc gacatggagc gtttcaagtt
     7441 tcttgccggt gaggtgacaa agctgaacga taaactcaac tatacatacg gaggcacggt
     7501 caagtgac
//
LOCUS       DASSFP010000378         5118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_709019, whole genome shotgun sequence.
ACCESSION   DASSFP010000378 DASSFP010000000
VERSION     DASSFP010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709019"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFH91_08480"
     CDS             <1..346
                     /locus_tag="VFH91_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6863071.1"
                     /translation="NWREIDPRRTEGPIPVECRSASCGTCWVGVLGGAEKLSDVAARE
                     GKKIKEFGYIDSPEPKPLVRLACQAQAHGAVSIVIPPWNGVFGKYLRSQAANAETPIA
                     SESSDGASAVDF"
     gene            478..1365
                     /locus_tag="VFH91_08485"
     CDS             478..1365
                     /locus_tag="VFH91_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6863072.1"
                     /translation="MTNSTKLSPLLYVVAAALLWSTGGLFIKWTTLSGLELSFFRSFF
                     ALFTVAFFTRREGFGINLVSAAGALLYAVLLILFVLATKETTAANAIFLQYTAPVYLL
                     IFEPLFYKEKFRLRDLITVLICLGGMTLFFVGQLRPQDVKGNILALASGFCFALYFLL
                     LRHPRARKVNRASSVIYGNLLAVLLTAPWGMAAIKSINSHDMLSVIYLGVVQIGVAYT
                     LFTIAMARGVRSLDAGIMGYIEPVLNPVWVFLVLGERPSQWALLGGAIIILAVVIHML
                     ADAREKRQASSPPLESAHG"
     gene            complement(1362..1934)
                     /locus_tag="VFH91_08490"
     CDS             complement(1362..1934)
                     /locus_tag="VFH91_08490"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET6863073.1"
                     /translation="MVWVNTSPLVKTYTLEEFWELPDPPDHSKVELIAGVLYMTPPPG
                     YLHDNAVSRINRLLSAHLLKTDDQGTLYVPRAAIWTSTNTYLEPDLFYVSKETESTME
                     ENFRTTADLVVEVVSPGSAIYDRNTKADTYAALGVKELWLVDENAQIIEKRVLDGGRY
                     QRNEVHEEKDMFTSSLLPDFTFSVEEVFAR"
     gene            complement(1987..2502)
                     /locus_tag="VFH91_08495"
     CDS             complement(1987..2502)
                     /locus_tag="VFH91_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863074.1"
                     /translation="MRSFREVLRSGEAHCLEAAVSAAVILEQHGYPPILLDLESQDLL
                     DHVLFVFQENGLWGAVARSRDFGLHGRRPVFRSIRDLVWSYFDPYVDLTGRIKGYGVT
                     SLYELGSYDWRFSGRKMTKIEDHLREIPHKRLRSSDLRYASLLTRYQEYKKRYPNRSP
                     RYYRGREKWMM"
     gene            2670..3035
                     /locus_tag="VFH91_08500"
     CDS             2670..3035
                     /locus_tag="VFH91_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863075.1"
                     /translation="MRSSLRGSEVDLPESIQNVRLTALINFNGSDHYLFLQPEMNSNM
                     NQQATAFQKTRAEQETENEANRLIEQVESALAIVAVRSPADDDSLSASADRIERAARD
                     FADALRQLSQHRKDTEQGS"
     gene            3090..4709
                     /locus_tag="VFH91_08505"
     CDS             3090..4709
                     /locus_tag="VFH91_08505"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HET6863076.1"
                     /translation="MASIASESVTENLGRKNTNYELNNYGITPGRVYWNLNPSELYEE
                     IARRNEGVLSDHGALIVDTGEHTGRAAKDKAIVREPESADKVFWGDVNKDFPQAKFNA
                     LRDRMMAHAGGRDLFVQDTFVGADKNFRLPIRVINELAWHSLFARTMFISRVEAEPHT
                     PQFTIINLPSFQADPERDGTRSPTFILIDFSQKLVLIGGTSYAGETKKSAFTLMNYLL
                     PQRGVMSMHCSANVGSDGDVAIFFGLSGTGKTTLSADPERRLIGDDEHGWSDDGVFNF
                     EGGCYAKVIKLSAEAEPDIYKTTRMFGTILENVVYDSETRVIDLDDAAKTENTRASYP
                     LTSISNIVPAGRAGNPRNIIMLTADAFGVLPPVSRLTPEQAMYHFLSGYTAKVAGTER
                     GIKEPEATFSTCFGAPFMVLHPGVYADLLGQKMAQHNAACWLVNTGWSGGPYGVGQRM
                     KISHTRAMIRAILNGELASVETRPDPIFGVNVPLSCPGVPSEVLQPRNTWADKDAYDR
                     QAEDLADRFNKNFKKYEAGVSEAVRAVAPKL"
     gene            complement(4804..>5118)
                     /locus_tag="VFH91_08510"
     CDS             complement(4804..>5118)
                     /locus_tag="VFH91_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863077.1"
                     /translation="ARRVLSQVLIQSVESAQAGLRNNKVDLAVINFQLAKEIDTKNPN
                     LVFELARVFALKRDKRSTLETLEEAVSLGFKDSDRLKSEEAFSFVAADPRFQKLSESL
                     NP"
BASE COUNT         1269 a         1279 c         1276 g         1294 t
ORIGIN      
        1 aaattggaga gagatcgatc cgcgcaggac cgaaggccca attccggttg aatgccgttc
       61 agcgtcgtgt ggcacttgtt gggtgggggt tcttggaggc gccgaaaagt tatcggacgt
      121 cgccgcgcga gaaggcaaaa aaattaagga gtttggatac atcgactcac ccgagccaaa
      181 gcctctagtc cggcttgcct gtcaggccca ggcgcatggc gctgtttcta ttgtgattcc
      241 accctggaac ggcgtcttcg gtaaatacct ccgctcgcaa gcagccaatg cagaaactcc
      301 catcgcaagc gagagttcag acggagcttc ggcggtcgat ttctaatgta cttctgattg
      361 gactcatcat tgctcatttg ttcctttctc attggcaagg tgctcttagt tagcggcagc
      421 aaattgaaat ccagcaaatg agaaatgaaa atagtctttt cttaatcgaa accacccttg
      481 accaactcca ctaagctctc gccgctactc tacgttgtgg ccgctgccct gctctggagc
      541 acggggggtc ttttcatcaa atggaccaca ctctcaggtc tcgaactgtc atttttccga
      601 tcgtttttcg cactcttcac tgtagccttt tttacgaggc gagaaggatt tggtattaac
      661 cttgttagtg cagccggcgc gctgctttac gctgttctct taatcctctt tgtacttgcc
      721 actaaagaaa ctaccgccgc aaatgcaatt tttcttcaat atactgctcc ggtttatttg
      781 ctgatcttcg agccactctt ttacaaggag aagtttcgcc tccgagatct gataacggtg
      841 ctgatctgtc tcggaggaat gacgctgttc tttgtcggcc agttgcggcc ccaggacgta
      901 aaaggaaaca tcctcgcact cgcttctgga ttttgttttg ctttgtactt tctgttgttg
      961 agacatccgc gcgcgaggaa agtcaaccgt gcttcgtcgg tcatttacgg aaacttgctg
     1021 gccgtcttgt taacagctcc ctggggaatg gctgcaataa agagtattaa ctcgcacgac
     1081 atgttgagcg tgatctatct cggcgtggtc cagatcggtg tggcctacac actgttcaca
     1141 attgctatgg ctcgtggtgt tagatctctg gacgcgggaa ttatgggata catcgagccg
     1201 gtcttaaatc cagtttgggt gtttttggtt ctgggtgaga gaccctcgca atgggcttta
     1261 ctcggcgggg caattattat tctcgccgtt gtaattcaca tgctcgctga cgctcgcgag
     1321 aagagacaag caagctcgcc gcctttggag tcagcacatg gttagcgagc gaacacttct
     1381 tctaccgaga aagtgaagtc aggcagtaag gacgaagtaa acatatcttt ctcttcgtgg
     1441 acttcgttgc gttgataacg accgccgtcc aaaacccttt tctcaataat ctgcgcgttc
     1501 tcatcaacta gccacaattc cttcacacca agtgcggcgt aagtatccgc cttagtgttg
     1561 cggtcataga ttgccgaacc gggcgagacc acttcaacaa ctaagtccgc ggtggtccgg
     1621 aagttttctt ccatcgttga ttcggtttct tttgacacat aaaagagatc gggttccagg
     1681 taggtattag tactggtcca gatcgcggct cgtggaacat acaacgttcc ctggtcatct
     1741 gtcttgagaa ggtgcgctga caagagccga tttattcgac taacagcatt atcgtgaaga
     1801 tacccgggag ggggtgtcat atagaggact cctgcgatta gttcgacctt ggagtggtcg
     1861 ggcggatctg gtagctccca gaactcttcg agcgtatatg tcttaactaa tggagatgta
     1921 tttacccaga ccatttgcga aactccaatg aggattggtt gagaggagta tagactaaac
     1981 tggaaattac atcatccatt tctcgcggcc acgatagtac ctcggcgagc ggttcggata
     2041 tctcttcttg tactcctgat aacgcgtcag cagggatgcg tatctcaaat ctgacgaacg
     2101 tagcctttta tggggaatct cgcggagatg atcttcaatc tttgtcattt tccgacccga
     2161 gaatctccag tcgtagctcc caagctcgta taagctcgtc acaccgtatc ctttgatcct
     2221 tccggtcaga tcaacatacg ggtcgaaata actccagacc agatctctaa ttgaacgaaa
     2281 cacaggtcgt cgcccatgca atccaaaatc gcgggaacgg gccacagcac cccacagacc
     2341 gttctcctgg aaaacgaaca ggacatggtc cagcaagtcc tgagactcca ggtccaggag
     2401 tatgggcgga tagccatgtt gctcgagaat cacagctgca cttacggcgg cctcaaggca
     2461 gtgcgcttct ccggatcgca gaacctctct gaacgagcgc atcgtggctc ccgtacgttc
     2521 ccagttgtac ggcatttgtg tgaaaaatcg ctggactctg gctggcgagt tcagcttgct
     2581 aacgaccttc cattcggacg cggtaaactt tttttctgag ggtttttgga cttttgactc
     2641 aaacataaaa gtaatgccaa cttaactctt tgagatcgtc tttgcgcggc agcgaagtgg
     2701 acttgcctga atcgattcaa aatgtaagac ttactgcctt aataaacttc aacggcagtg
     2761 atcattattt atttttacaa cctgaaatga actcaaacat gaaccagcag gcaacagctt
     2821 tccaaaagac ccgggccgag caagaaacgg aaaatgaagc aaaccgactg atcgaacagg
     2881 tggagtcggc tttggcaata gtagcagtgc gctcacctgc agacgacgac tctttaagcg
     2941 ccagtgccga ccgaatcgag cgggccgcac gtgatttcgc cgatgcgtta cgccagctct
     3001 cccaacatcg taaagacact gagcaagggt cgtgagcgcc gcaacttacc atttgaacac
     3061 aatgaaatca cccgttggag aacatcgtta tggcttcgat cgcctctgaa tcagtaactg
     3121 aaaatctggg tcgcaaaaac acaaattacg aactgaataa ttacggaatt actccgggtc
     3181 gagtctactg gaatctgaat ccttcagaac tgtacgaaga gatcgccaga cgaaacgaag
     3241 gtgttctttc agatcatggc gcactgatcg tcgataccgg ggaacatacc ggtcgagctg
     3301 cgaaggacaa ggcaattgtg cgtgagcccg agagcgcaga taaggtgttc tggggcgatg
     3361 taaataaaga ttttccgcaa gcaaagttta atgcgcttag agatcgaatg atggcccatg
     3421 ccggcggtcg agatcttttt gttcaggaca catttgttgg cgccgataaa aacttccgtt
     3481 tgccgattcg cgtaattaat gaattggcct ggcattcatt gtttgcgcga acgatgttta
     3541 tcagcagagt agaagctgaa ccgcatacac ctcaatttac aataattaac cttcccagtt
     3601 ttcaggcaga tccggaacgt gacggcacgc ggtcaccaac gtttattctg atagatttta
     3661 gtcagaaatt ggtgctgatt ggcggcacct catatgccgg cgaaacaaag aagagtgctt
     3721 tcactctaat gaactatctg ctgcctcaac gcggagtgat gagcatgcat tgctctgcga
     3781 atgtcgggtc cgatggtgac gttgcgattt tcttcggact ttccggaaca ggaaaaacaa
     3841 ccctctcagc cgatcccgaa cgcagactga tcggagatga cgagcatggc tggtcagatg
     3901 atggagtctt caattttgaa ggtggctgtt acgccaaagt tataaaactg tcggccgagg
     3961 ctgaacccga tatctataag acgaccagaa tgttcggaac cattctggaa aatgtcgttt
     4021 acgattcgga aaccagagtc atcgatctgg acgacgcagc caagactgaa aacacccgcg
     4081 cctcgtatcc gctaaccagt attagcaaca ttgttccagc aggtcgtgcc ggcaatccac
     4141 gcaatatcat aatgcttaca gccgacgcgt ttggggtact gccgccggtg tcacgcttaa
     4201 ctccagagca ggcgatgtat cacttcctgt ctggatatac agcaaaagtt gcgggtactg
     4261 agcggggaat caaggaacca gaagcaacat tctcgacctg ttttggggct ccgttcatgg
     4321 tcctacatcc cggagtctat gccgatttgc tgggccaaaa gatggcccaa cataatgctg
     4381 catgctggct ggttaacacg gggtggagcg gcggacccta cggcgtcggt caacgcatga
     4441 agatctccca tactcgtgca atgattcgcg cgattttgaa tggagaacta gcatccgtcg
     4501 aaactcgtcc ggatccaatc ttcggggtga atgttccctt gtcatgtccc ggcgttccgt
     4561 cagaagtttt gcagccccgt aacacctggg ccgacaaaga tgcttatgat aggcaggctg
     4621 aggatttggc ggatcgtttc aacaagaatt tcaaaaagta tgaggccgga gtttctgaag
     4681 cagtccgtgc cgtcgctcca aagttgtaag gcgtccctcg tgggcgccct ttcttgagag
     4741 atccgacttt tccgaagatt ctaaggcaca agaaggggcg cccacggagg gatgccccaa
     4801 caattacggg ttgagtgact cagataactt ttggaaccgg ggatctgcgg ccacaaaact
     4861 aaacgcttct tcggatttca acctgtcaga atctttaaaa cccagcgaca cggcctcctc
     4921 gagtgtctca agtgtcgatc tcttatcccg ctttaacgca aacactcgcg ccaactcaaa
     4981 aaccaaatta ggatttttcg tatcgatctc tttagccagc tgaaagttga taacggcgag
     5041 atcgaccttg ttgttcctga gacctgcctg cgcactctcg actgattgaa taagaacctg
     5101 cgagagcacg cgtcttgc
//
LOCUS       DASSFP010000379         8546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_709119, whole genome shotgun sequence.
ACCESSION   DASSFP010000379 DASSFP010000000
VERSION     DASSFP010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709119"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFH91_08515"
     CDS             <1..197
                     /locus_tag="VFH91_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863078.1"
                     /translation="HELATTLTDCLMRRTVLGLNSDLGITVAETAAKVARAHLCWTED
                     RLLKELSDYRAYLDRFAVGK"
     gene            complement(205..774)
                     /locus_tag="VFH91_08520"
     CDS             complement(205..774)
                     /locus_tag="VFH91_08520"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HET6863079.1"
                     /translation="MFSLLEPSDETVRDLIARQQDVSFTYERVGATRTTPPQDFIVDH
                     NRIEIGRGEKAFKTGIDAIRNWKQFDLGWVSIVPVGVKLEVGATVAVKVRALGTWSLN
                     ASRVIYLIEEPRRFGFAYGTLPDHVESGEERFSVEWLADDSVWYDIFAFSRPRHPLVK
                     INVPLARMLQKRFARDSKRRMLAEVRKNQ"
     gene            complement(787..1725)
                     /gene="xerD"
                     /locus_tag="VFH91_08525"
     CDS             complement(787..1725)
                     /gene="xerD"
                     /locus_tag="VFH91_08525"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET6863080.1"
                     /translation="MSYNSRRFDINHAVNQVRDFTKEFLNYLQVEKGLARHSLDSYRR
                     DLAKLEQWAIANKQSTVDLTRADLRKWIASLSRDGLAPSSVARAVSAARGFYKFLMLD
                     GHIKHHPAEDLDTPQRFNYLPKFLTEDEISKLFATPNVATPDGIRDRAILEVMYASGL
                     RVSEMVNLKQADVDLLAGLLTCHGKGSKERRVPLGKSAVHWLQQYFSVKASYGKSSYP
                     QVFLYNGKPLTRQLAWSIIKKHADKAGIEKVSPHTLRHSFATHLLQHGADSRSVQALL
                     GHSDISTTQIYTHMSDIHLRSAYNRHHPRAKLKPHD"
     gene            2131..7782
                     /locus_tag="VFH91_08530"
     CDS             2131..7782
                     /locus_tag="VFH91_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6863081.1"
                     /translation="MIGQRLSNRYDIRAELGRGGMGVVYRAHDPLLNREVAIKLIPPT
                     LLSSETEQRFQREAQLVAQMDHPAVVPIFDFGKDEGSLFFVMPIVQGTTLRWYQRDNP
                     LTLGEVLDIGIQAAEALEYSHSRGVIHRDVKPENIMVVREDGARIRVRIMDFGLARGA
                     TESRITKTGTIAGTLSYMSPEQVAGGAIDHRSDIYSLGTVLYECLSGEPPFSGELQSV
                     LYRIVHEIPQPLSSVGAEINEDLESLILTCIAKEPGKRPPRAGDLAESLRRCQTRLHH
                     SDLSKSVVLTKTMMMPRAALSPFIGREKEQAELQKRLNAAISGECQFVVVSAEPGVGK
                     TRLLDEIESLAKARKLLVLHGRSIEQDGAFPYQGFCEAIQEFFRQRDTASSPDNAIEL
                     SDVAPDLVALFPMLTEISAIRSAASGDSKLTHTGGLGSQGPENRTQVFELLARTLTRL
                     AAGKPLILFLEDLHAAEISIEALQYIVRRLGPTSTLIVGTYRSSEVDRSHPLLRMLDS
                     FRGDRRFASIVLEPFTPVEHRLFLETLIGGPKLSDTLVTKLYEGTEGNPFFTKELVRS
                     LLDSGGISKDQTGAWSLGASTDLSTGDMPATIQQAVEKRIERLPEELREVLSIASVMG
                     KAFDFRDLEVLAGQKDIEDAIDRLVEEGLLEEERESRGDRLVFSSGVVRDVLYGAISR
                     RKRRSLHRKYAEEIEKRHGGRLERVYPQLVHHFSQGDVPDKTVEYGLRMAKAALDAFS
                     SEEAARASKTVLEFLDDEWEGDRSLEGDARMLLAQSYRMGGDVDSALREAESAARILS
                     SEKHLARSVTSLLFAAETAWQARKVEETTRLVQEVMDDARAVGDKESLRHVLSLAATL
                     ANLRGEYEKANEYLEESGSLAPAPKETERHEEIPRGGRLVVAMASPLGDIDPVNMELV
                     EEQEILANVFETLLATDLEGNLIPALCEKWEVSEDGTSVFFTLKEGARFQNGEPLNAA
                     VVKRSLERSIRVARELPPGLAAIRDAATFARNDDAELTSLIVHSSNKLEIQLPEPLSI
                     YPSLLTDFKTGITLQPEGAKDGAILFGTGPFRMTSYSPDRILLERNDSYWKGSFANLD
                     EIEFRAGLSASDIAAGLKSGAIDLARDLSPQDLEEFLRDQRFRGGLVESPRKNTYFLI
                     FNSTSGSVAQKPELRRALSETIRTHDLVWQTLGRFAQPAVCLIPPGMLGHDPGRRRQT
                     LTRDEAVAMLRDAGFTETIPLRAAVHPLFLDRYKSLLTTLLAIWSELGVQVEVATPSM
                     ASYLESFQNSTGIDLFIGRWNADYDDPDDFTYGLFHSRVGLYRPYISASDGDQTLEEA
                     RTESRPAVRATLYRKYESFLQESGMVLPLFHDVDYRLANSKILGLSLRGSAPYVTYTD
                     IGKLESAAKTTDAVRASVGTLEIPITGTLHHLDPALAGTVEDTEVLPSIYDTLTRDVG
                     GARIAPWLAAEVIPEQGGKRYRFRLRDDVRFHDGRKLTARDVRFSFERMLLSTESLGR
                     YFYSPIRGAKALLNGEDTALTGFRIHSAEEFTIELEEPISFFPALISYHVAAIVPEGS
                     DKFGPSWQEGSVGTGPFRVVKFEPGVRLELERNKSYWRPGYPKVERLNFDFDVAPADI
                     LSEFRAGRFSLASDLLPADAEALRREPEYASGYHEIPNLMTYYAVFNCNRGPLKDKRV
                     RQRLARSINVANIVRQTLGRLAVPAQGLIPPGLLGHDSAYATRISIPSVTSSGESQEE
                     IELTAAVNPVFFGEYASLAREIARSAAEQKVRIRTVNKTMEEWLEAVSQGTVDVVLGR
                     WGADYPDADTFVNILATKNGLLGKLCGSSEVDRLIAKGRTETVPTVRHGIYRQVEEII
                     VRDSLLLPLFHEQSYRFARPEVQGVSLSYGSITVDYSSLRL"
     gene            complement(7833..>8546)
                     /locus_tag="VFH91_08535"
     CDS             complement(7833..>8546)
                     /locus_tag="VFH91_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6863082.1"
                     /translation="VPLILNIHGGPAGVFQQNFIGGRGVYPLATFAARGYAILRANPR
                     GSSGYGVDFRRANIKDWGVGDYQDLMTGVDKVIEMGVADPDRLGVMGWSYGGFMTSWI
                     VTQTHRFKVASAGAPVTNLMSFNGTSDIPSFVPDYFGGQSWDGIEIYQKHSPMFNVKG
                     VTTPTMIQQGDADVRVPISQGYEFYNALKSQGVPTRMLVLPRQPHGPTEPKMQLAAMK
                     ANLEWFDKYLSGKAETTKN"
BASE COUNT         2042 a         2125 c         2288 g         2091 t
ORIGIN      
        1 agcatgaatt ggcaacgacg ctcacagact gtttgatgag gcgaacggta ctgggactca
       61 atagcgatct gggaattaca gtggctgaaa ccgcggcaaa agttgcccgg gctcatttgt
      121 gctggactga ggatcgattg ctcaaagagc tttcagatta tcgggcttat ttagaccgct
      181 tcgcagttgg aaagtagcgt aaggctattg gttcttccga acttccgcga gcatgcggcg
      241 ctttgagtct ctggcgaacc gcttttgcag catccgagcc aatggaacgt tgatcttcac
      301 cagcggatgt cgtggacggg aaaacgcgaa tatgtcgtac cagaccgagt catcggcgag
      361 ccattccacg ctaaaacgtt cttcgcccga ctcgacatga tccggcaagg tcccgtatgc
      421 aaacccgaac ctgcgcggct cttcaatcaa atagatcacg cgagacgcgt taagagacca
      481 ggtgcccagc gcgcgaactt tgacggcgac tgttgcgccg acctcaagct tcactccgac
      541 aggaacgatg ctgacccacc cgagatcgaa ttgcttccaa tttcgtattg cgtcgattcc
      601 ggttttaaag gctttctcgc ctcgaccgat ctcaattcga ttgtggtcca cgatgaagtc
      661 ctgcggcgga gtggtcctgg tagcgccgac tcgctcataa gtaaaagaca catcttgttg
      721 tcgggcgatt aagtccctta ccgtctcgtc agaaggttca agtagcgaaa acatttaacc
      781 tggaaatcaa tcgtgcggct ttaacttcgc ccgcgggtgg tgtcggttat aggccgatct
      841 caaatggatg tcagacatgt gcgtgtaaat ttgagtggtc gaaatatcac tatggcccaa
      901 taacgcctga actgagcgcg agtcggctcc atgttgtaaa agatgggtcg caaagctgtg
      961 ccgcagtgta tgaggcgaaa ctttttcgat accggccttg tccgcgtgct ttttaatgat
     1021 ggaccatgca agttggcgcg ttagtggttt cccgttgtac aaaaatactt gtggatagct
     1081 ggacttaccg tacgacgcct taaccgagaa gtattgctgc aaccagtgaa cagcgctttt
     1141 ccccaaaggt actctccgct ccttgcttcc tttgccgtga cacgtcagca ggcccgcgag
     1201 cagatctaca tcggcctgtt ttaggttcac catttccgag acgcgtagac ctgaagcgta
     1261 catgacttcg aggattgctc tgtccctgat tccgtccggg gtcgcaacgt tgggagtcgc
     1321 aaacagttta ctgatttcgt cttcagtaag aaatttcggt aggtaattaa aacgctgcgg
     1381 tgtgtcgaga tcttccgccg ggtggtgctt gatatgacca tccagcatca gaaacttata
     1441 gaagccgcgg gcggcgctga ccgcgcgtgc aactgaagaa ggcgcaagtc cgtcacgtga
     1501 caaactcgcg atccactttc gcaggtcagc ccgagtgagg tcaacggtcg actgtttgtt
     1561 agcaatggcc cattgctcaa gttttgccag gtcacgccga taactgtcga gggaatgcct
     1621 tgcaagccct ttttcgactt gcaggtagtt gagaaattct tttgtaaagt cgcgaacttg
     1681 attcacagcg tgattgatgt cgaacctgcg ggaattatag ctcataccga aacgggcgat
     1741 tacagctgga aagaaagatc atccacagat tacgccgatt acccagcttt cttcagagaa
     1801 aggtacggcg caaaggcgtt ctagtaaaga atctttgtaa tcggcgtaat ctgtggattg
     1861 tattcttgag ttttcataag agtctctgaa ggcggtggtg tccgcgattg aggcactcgt
     1921 tgcaacacat cttgctaggt gcggcagcga aatctcagaa cattttcacc tgtcgctata
     1981 catctcctct cgtcttgtcc tatgatgcgc acgttcattt ttatgacgtt ttatctgctg
     2041 ggtccggagg ctttacgacc gcagtttact taccattaat caccttcaac aatctgtaat
     2101 cgcactcatg ttattcctcc tggagagaag atgatcggcc aaaggctttc caatcgttac
     2161 gacatcagag cagagttggg ccgtggtgga atgggcgtcg tttatagagc gcacgatcca
     2221 ctattgaacc gtgaagttgc aatcaaactt attcctccga cactactaag ttccgaaaca
     2281 gaacaacggt ttcagcgcga ggctcagctg gtggcccaga tggatcatcc ggcagttgta
     2341 ccgatcttcg actttggtaa agacgaaggc tctttattct ttgttatgcc gatagtgcag
     2401 ggaactacgc tgcgctggta tcaacgggat aatccgctaa cgctgggcga agttctggac
     2461 attggtattc aagccgctga ggcgctggaa tatagtcatt ctcgtggcgt aattcatcgt
     2521 gacgttaagc ccgaaaatat tatggtggtc cgtgaagatg gagccagaat tcgggttcgc
     2581 atcatggatt tcggtcttgc ccgtggagcc acagagagtc ggatcacgaa gactggaaca
     2641 atcgccggca cgctctccta catgagtcct gagcaagtag cgggcggcgc catagaccat
     2701 cgttccgaca tctattcgct tggtaccgtt ttgtacgaat gtttgagtgg ggaaccacct
     2761 ttctcgggcg aactgcagtc tgtgctctat cgcatcgtgc atgaaatacc gcagccactc
     2821 agttcagtag gcgcagagat taatgaggat ctcgagtccc tgatcttaac ctgtatagct
     2881 aaagagcccg gcaaaagacc cccgcgagcc ggtgacctcg cagaatcgct ccgacgctgc
     2941 cagacacgct tgcatcacag cgatctgagt aagtctgtcg tactgaccaa aacgatgatg
     3001 atgccgagag ctgcattgtc tccgtttatt ggtcgcgaaa aagaacaagc tgagttacaa
     3061 aaacggctca atgcggcaat cagtggcgag tgccagttcg ttgtcgtctc cgccgaacca
     3121 ggcgttggta aaacacgtct gttggatgag atcgaaagtc ttgcgaaagc tcgtaagcta
     3181 ctagttctgc acgggcgctc aatcgaacag gatggagctt ttccctatca gggattttgc
     3241 gaagcaatac aggagttctt tcgtcagagg gatacggcga gctctccgga taacgccatc
     3301 gagctttcgg acgtggcacc cgatctggtt gcgttattcc caatgctgac agagatctcg
     3361 gcaattcgta gtgctgcttc gggcgattca aagttaacgc atacaggtgg acttggttcg
     3421 cagggaccgg agaacagaac gcaggttttc gaactgctcg ctagaactct aacgcgtttg
     3481 gccgcgggca agccgctgat tttattcctc gaagatttac atgcagcaga gatctcgatc
     3541 gaagctttgc aatatattgt gaggcgactt ggaccaacgt cgactttgat cgttggtaca
     3601 tatcgctcaa gcgaagtcga ccgttcacac ccgttgcttc ggatgctcga cagcttccgc
     3661 ggagatcgtc gctttgcgtc tattgtcctg gagcctttta cacctgttga gcatcgcttg
     3721 ttcctggaaa ctctcattgg tgggccaaaa ctctctgaca ctctcgtaac taaactctat
     3781 gaaggtaccg agggcaatcc gttctttaca aaagaactgg tgcgctcgtt gctcgactcc
     3841 gggggcattt cgaaggacca aactggcgca tggagtttgg gcgcttccac cgatctttcg
     3901 accggtgaca tgccggccac cattcagcaa gcggttgaga aaagaatcga gcgtttgcct
     3961 gaggaactgc gcgaggtgct ctcgatcgct tctgtaatgg gcaaagcatt cgactttcgc
     4021 gacctcgagg tcctggctgg tcagaaagat attgaagacg caatcgatcg attggtcgag
     4081 gaaggactgt tggaggaaga gcgagagtca cgaggcgatc ggctggtctt ctcaagcggg
     4141 gtggtgcgcg atgtccttta cggcgctatt tcccgtcgca aaagaaggtc tttgcatcgc
     4201 aaatacgcgg aggaaattga aaagcggcat ggcggaagac ttgagcgcgt atatcctcaa
     4261 ttggtccacc atttctcgca gggcgacgtg cccgacaaga ctgttgagta cgggctgcga
     4321 atggcaaaag ccgcgctcga tgcattcagt agcgaagaag cagctcgtgc gtcaaaaacc
     4381 gtactcgagt tccttgacga tgagtgggag ggagatcgtt ctttagaagg cgatgcccgg
     4441 atgttgctcg cacaatcata cagaatgggc ggcgacgtcg actcggcgtt gagagaggcc
     4501 gaaagtgcgg cacgtattct cagcagcgag aagcatctgg ctcgctctgt cacctcattg
     4561 ttgtttgccg cagaaacagc gtggcaagct cgaaaggttg aggagacaac gcggctggtc
     4621 caggaagtga tggacgacgc gcgagctgtc ggggataaag aaagtcttcg tcacgtttta
     4681 tcgttagcgg ccacgcttgc aaatctacga ggcgagtacg agaaagcgaa cgaatatctt
     4741 gaggaatccg gttcacttgc tcccgcgcca aaagagaccg agcgccacga ggaaatacca
     4801 agaggcggga gattagtagt agcgatggct tctccactcg gtgacatcga tcccgtgaac
     4861 atggagctgg tagaagaaca ggaaatcctt gcaaatgtgt ttgagacatt gctggcgacg
     4921 gatctggaag gcaacctgat ccctgcacta tgcgagaagt gggaagtttc agaggatgga
     4981 acatcagtct tcttcacgct taaagaaggc gcgcgttttc aaaacggcga accactgaac
     5041 gctgcagtgg tcaaacgatc tctcgaaaga agcattcgtg tcgcgcgcga gttgccgccc
     5101 gggttggccg caatacgcga cgccgcgacc tttgccagga atgatgatgc agagttgacc
     5161 tcgcttatcg tccattcatc taacaaactc gagatccaac tacctgaacc actttctatt
     5221 tacccgtccc ttctaacaga cttcaaaaca ggaatcacgc tccagccaga aggtgctaag
     5281 gacggcgcga ttctattcgg gactggaccg tttcgcatga cttcgtatag ccctgatcga
     5341 atcttgctgg aacgaaatga ttcctactgg aaaggctctt tcgcaaatct cgatgagatc
     5401 gaatttcgtg ccggcttgag tgcgtcagac atcgcggcag gattaaagtc cggagcaatc
     5461 gacctcgctc gtgatctctc gccgcaggac ctggaagagt ttctgcgcga tcagcgtttt
     5521 cgtggagggc ttgttgagtc tccacgtaag aacacctatt ttttgatctt caattcgaca
     5581 agtggatcag tggcccaaaa gcctgaattg cgtcgcgcgc tttcggaaac gattcgcacg
     5641 cacgatctgg tttggcaaac gctcggtcgg tttgcacagc cggcagtctg tttgattccc
     5701 ccgggtatgc tgggtcacga tccaggcaga aggcggcaga ctctgacacg tgatgaagca
     5761 gtagcaatgt tacgtgatgc gggattcacg gaaacaatac cactgcgagc ggcggtccat
     5821 ccactctttc tcgatcgcta taaatctctg ctaaccactc tgctcgccat ttggtctgag
     5881 ctgggagtgc aggtagaagt cgcaactcca tcaatggctt cttacctgga gtcgttccag
     5941 aacagcaccg gtatcgatct gttcatcggc agatggaatg ccgactatga cgatcctgac
     6001 gacttcactt acgggctctt ccattcacgc gttggtttat atcgtccgta catttcggcg
     6061 tctgatggag atcagacact tgaagaagcc cggacggaaa gtcgtcccgc cgttagagcg
     6121 actctttatc gaaagtacga aagctttttg caagagtcgg gcatggttct accgttgttc
     6181 cacgacgttg attatcgtct ggcgaactcg aaaattctgg gactaagctt acgaggcagc
     6241 gcaccttacg taacttatac tgacatcgga aaactcgaat ccgcggccaa aaccacagat
     6301 gccgttcgcg ccagcgtcgg gactttagaa attccaatca ccggaacatt gcatcatctt
     6361 gatccggcat tagcgggcac agtcgaagat acagaggttt taccgagtat ttacgacacc
     6421 ctaactcgag atgtgggtgg cgcgaggatc gctccgtggt tggctgctga ggtgattccg
     6481 gagcagggtg gcaagcgcta caggtttaga ttgcgtgacg acgtgcgctt tcatgacggt
     6541 cgcaaactaa cagctcggga cgttcggttt tcgtttgaac gaatgttact gagcactgag
     6601 agcctgggcc gttacttcta ctctccgatt cgtggtgcta aggcgctact caatggagaa
     6661 gacaccgcat taaccggatt ccgcatacat tctgcagagg aattcaccat cgagttggag
     6721 gagcccatat catttttccc cgctcttatc tcgtatcacg ttgcggcgat agttccggag
     6781 ggaagcgaca agtttggtcc aagttggcag gaaggatctg tcggaacagg acctttccgc
     6841 gtagtcaagt ttgagccagg agttcgcctt gagctcgaaa gaaacaagag ttactggcgt
     6901 ccgggctacc cgaaagtaga acgactcaac tttgacttcg atgttgcacc ggctgatatt
     6961 ctttctgagt ttcgagcagg caggttttcg ctagcctcag atcttttacc agcagacgcg
     7021 gaagccttgc gacgcgagcc tgagtacgct tctggctatc acgaaattcc caacctgatg
     7081 acatactacg cagttttcaa ttgcaatcgt ggtccattga aggataaacg cgtcagacag
     7141 agattggcgc gatcgatcaa cgttgcgaat atagttcgtc aaacgcttgg acgtcttgct
     7201 gtcccggcgc aggggttgat cccgccaggc ttgctgggcc atgactccgc ttatgcgacc
     7261 agaatctcga taccctctgt cacttcctcc ggtgaaagtc aggaagagat tgaattaacc
     7321 gctgcggtaa acccggtgtt ctttggcgaa tatgcatccc tggctcgtga aattgcacgt
     7381 tctgcggctg aacaaaaagt gagaatacgg acagtaaata agacgatgga ggagtggctc
     7441 gaagcggtgt cgcagggaac tgtcgatgtg gtgcttgggc gctggggcgc tgactatccg
     7501 gatgcggata cgttcgtcaa tattctggca actaagaatg gcttgctcgg taagttatgt
     7561 ggcagtagcg aagtcgatcg actaatcgca aaaggacgaa ctgagactgt tcctacggtt
     7621 cgtcatggta tttaccgaca ggtggaagag atcattgtgc gcgacagtct gttgctgccg
     7681 ctgtttcatg agcagagtta tagatttgca agaccggaag ttcagggtgt gtcgttatcg
     7741 tatggatcaa taaccgttga ctattccagt ctgaggttgt agccaggtat caattatcaa
     7801 ttgacacttc tcaattaata attgccactt agtcagttct tcgttgtctc cgccttacca
     7861 ctaagatact tatcaaacca ctccagattg gctttcattg cggctaactg catcttcggt
     7921 tctgttggac catgtggctg tcgtggcagt accaacattc gtgtcggcac accctgagac
     7981 ttcaaggcgt tatagaactc gtatccctgc gaaataggga ctcggacatc ggcgtcgccc
     8041 tgttgaatca tggttggggt agttacacct ttgacattga acattggaga gtgcttttga
     8101 tagatctcaa ttccatccca cgactgaccg ccaaaatagt caggtacgaa cgatggaata
     8161 tctgacgttc cattgaagct catcaggttg gttaccggag cgccggctga tgcgacttta
     8221 aatcggtgag tctgggtgac gatccaggac gtcatgaatc caccgtaact ccaacccatc
     8281 acgccaagac ggtcgggatc agcaactccc atttcgataa ccttgtcgac tccagtcatc
     8341 aaatcctggt aatccccaac gccccagtct ttaatgttgg ccctgcggaa gtcgactcca
     8401 taaccactcg aaccacgcgg atttgcgcgc agaattgcgt agcctctggc agcaaaggtc
     8461 gcgagaggat aaacaccgcg tccgccgata aagttctgtt gaaacactcc ggctggtcca
     8521 ccgtgaatgt tcaaaatcaa agggac
//
LOCUS       DASSFP010000380         6438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1304040, whole genome shotgun sequence.
ACCESSION   DASSFP010000380 DASSFP010000000
VERSION     DASSFP010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304040"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VFH91_08540"
     CDS             complement(<1..1211)
                     /locus_tag="VFH91_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HET6863083.1"
                     /translation="MSTLVSNCPACGAQIQFKSGQSIVVICEYCHSAVARTDRALKDL
                     GKVAELVETGSPLDVGVRGTWKGVSFELTGRAQLGHEMGGQWDEWYATFSNGWLGWLA
                     EAQGRFYLTFQYPAPENVQLPQFDQLQLGQSIQGLPTQVPMIVAETGRATALGAKGEI
                     PYLLTPRETYYYADLSGPEGAFGTLDYNESPPLIFLGHQVTLTDIGITATRAPERAER
                     QVGAAQLNCPNCGGPLELRAPDKTERVTCPNCNSLLDVNQGQLQFLKALEASWFQPVI
                     PIGTTGDVPEGKMTVIGAMARSVTIEGTQYFWGEYLLYNPQIGFRWLVHSDNHWNYVR
                     AVPPGEITESAKSANYTGGHYRIFQDATARVEVVLGEFYWKVETGESVRGVDYVSPPY
                     MLSKEVSTVYV"
     gene            complement(1257..1652)
                     /locus_tag="VFH91_08545"
     CDS             complement(1257..1652)
                     /locus_tag="VFH91_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HET6863084.1"
                     /translation="MNINLGTEWVIDANGCSDIALADLAQIQTVFARIINELQLHVIG
                     EIAWHQFASTGGISGLALLSESHLTCHTYPEFGAATFNLYCCRERASWHWESSLKEML
                     GAKQVTVRVLERLVKEDLKTSDELVTQAG"
     gene            complement(1996..2367)
                     /locus_tag="VFH91_08550"
     CDS             complement(1996..2367)
                     /locus_tag="VFH91_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6863085.1"
                     /translation="MKDLTIVIADDSPVVREHLKRALAQVEGCMLVGMAVDGVEAINM
                     VRALKPDIVVLDIAMPHRSGIDALKEIRREDPEIVIIMFTADPSDVLRDVCLEAGANF
                     YLDKSQLQELIDICEERYAVV"
     gene            2676..3092
                     /locus_tag="VFH91_08555"
     CDS             2676..3092
                     /locus_tag="VFH91_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863086.1"
                     /translation="MIQRLSTLICALVVALAMCGVANAQKIESLNATAKGKGTISTGV
                     DKLTIYSVMVVLKENGDAEFTFYCDLQLSAKGRWTANESSDQGINIEITGGVVSGNAT
                     GTGKLLLRPDGKSIDKLNIDAKSGSRTISIKFVADK"
     gene            complement(3106..3699)
                     /locus_tag="VFH91_08560"
     CDS             complement(3106..3699)
                     /locus_tag="VFH91_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863087.1"
                     /translation="MRNPLARNLCNFLSVITAATAISICSQNAKSEHRASQSQSTTNS
                     QSGASQSCDEIRENLQRLNQEMPRLRKRLAELEKDRQINTIQDMLTREEQRGEGLQLH
                     LIEIAEKEEPLQARMDQLNQNLKPERIESIMSGVGSVHPEEAREELRRRIINERGRVQ
                     MQLELLRQDKMRTLASLRTTDEAIQRLKLKLTEAIKE"
     gene            complement(3839..4759)
                     /locus_tag="VFH91_08565"
     CDS             complement(3839..4759)
                     /locus_tag="VFH91_08565"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET6863088.1"
                     /translation="MGTDANSRSDSDTLELLPPQWFFPGLSLPTSGVRVDIAGASHQG
                     HKRKNNEDHYLTLRFQRSLETLSTNIKDGHLNTAFEETGYGMVVADGMGGMAGGEIAS
                     RMALCKLVELVVNTPDWLMRFSEPEDVAKLTQRITQRFHQIDEAIKAHTKVEPSLVGM
                     GTTLTLGVSLGSHLLVGHLGDSRAYLMREDKMHQLTSDHTLAQALIDAGIIRPEDPST
                     RTTRHVLTTALGAIAKGTEPQVQRVDIKMGDQVLLCTDGLTEMVPDDKIASILGSTGG
                     SDSACQELVNAALDGGGRDNITVVVARYGE"
     gene            complement(4857..5339)
                     /locus_tag="VFH91_08570"
     CDS             complement(4857..5339)
                     /locus_tag="VFH91_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863089.1"
                     /translation="MKLSTSLLALILLFAVCVSCRLAESLTGDKNGGTVSSLWPDVPP
                     FQGATKADLEIPLGARLAIRAAMRGKLNFISFRTDKTAQEVKDFYSNERMKSAGWTPN
                     DKGCVGDTEDTKNHGAFCVFSKKDGQKEDGLAIVIAQDETHKDTNIFYARFDITKESK
                     "
     gene            complement(5378..6007)
                     /locus_tag="VFH91_08575"
     CDS             complement(5378..6007)
                     /locus_tag="VFH91_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863090.1"
                     /translation="MKRCPQCQFIYLNSDEQCDFDQTLLVSIDDETVEAAIRTNSSKV
                     SSPETVPSVTLKSPAKKLIPILVAALLLLILGLGLLGVYLIIKTRSSSNGAVNQITAV
                     TLPSGEKPQAVPNVRTPIVESTPEPTPKVAATPTRNTNSRAAVSIGPVSTSGVSNHGK
                     TSGKPIILLTTGGRIEADQVWRAKEGIWYRRDGIVTLLKRERVRAIVGQ"
     gene            6133..>6438
                     /locus_tag="VFH91_08580"
     CDS             6133..>6438
                     /locus_tag="VFH91_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863091.1"
                     /translation="MKSVRFTVSIILAVLFGACWAKKAPAPPASSGPSPADSPRVSET
                     SQPPSPPMGAPKAGPGELQTGQASGSYTAGGQTVELKYAYAGHAVRFSTESLVILLTD
                     "
BASE COUNT         1506 a         1615 c         1618 g         1699 t
ORIGIN      
        1 tgaacgtaga cagtcgaaac ttctttcgac agcatgtaag gagggctgac gtaatcgacg
       61 ccgcgcacac tctccccagt ttcaaccttc cagtaaaact caccgagcac aacttccacc
      121 ctggcagtcg cgtcctgaaa aattcggtag tgtccgcctg tatagtttgc actcttcgct
      181 gactcagtta tttcaccggg tggcaccgct cgaacataat tccagtgatt gtcactgtgg
      241 accagccagc gaaaaccaat ctgcggattg tagaggagat attcgcccca gaagtactgg
      301 gttccctcga tagtgacgct gcgtgccatg gcgccaatga ctgtcatctt cccttccggc
      361 acatcaccgg tggtgccgat tggaatgacc ggttgaaacc agcttgcttc gagagctttg
      421 agaaattgaa gttgtccctg gttgacgtcg agaagagagt tacagttcgg acatgtgacg
      481 cgctctgttt tgtcaggtgc tcgaagttcc aacggaccac cacagttcgg acaattgagc
      541 tgtgctgctc cgacttgtcg ttccgcccgt tcgggagccc gcgttgcagt aataccaatg
      601 tctgtcagtg ttacttgatg gcccagaaaa attagcggag gtgattcgtt gtagtcgagc
      661 gttccgaatg ccccttcagg tccggacaga tccgcatagt agtaagtctc tcgtggagtc
      721 aggagataag gaatttcacc tttggcgccg agtgctgtgg cccgcccggt ctcggcaacg
      781 atcattggga cctgagtagg aagtccctga atggattggc ccagctgaag ttggtcgaac
      841 tgtggaagtt gtacgttctc tggagctgga tattgaaacg tcagatagaa acgaccttga
      901 gcttcggcca accaaccgag ccagccgttt gagaatgtcg cgtaccattc atcccattgt
      961 ccgcccattt cgtggcctaa ttgagcgcgg ccggtgagtt cgaagctcac ccccttccac
     1021 gttccacgaa cgcctacgtc gaggggcgat ccagtctcga cgagttctgc tacttttccc
     1081 agatctttaa gcgcgcggtc agttcgagcg actgctgaat ggcagtattc gcagattacg
     1141 acgatggact gtccactctt gaattggatc tgggcgccgc aggcggggca gttcgatacc
     1201 agcgtgctca tctggtagtt ctccgccact ctgccgattt tgaaatcatt tgcgagttat
     1261 ccggcctggg taaccaattc gtcagacgtt tttagatcct cttttacaag cctctcgagg
     1321 acgcgaacgg taacttgttt cgcgcccaac atttctttga gcgaactttc ccaatgccac
     1381 gaagcgcgct cgcgacagca gtatagattg aaggtcgcgg cgccaaattc agggtaggta
     1441 tggcaagtta gatgtgattc tgagagtagt gccaaaccgc tgatgccgcc agtggaagca
     1501 aactgatgcc aggcaatttc tccaataacg tgtagctgca gttcattgat gatgcgagca
     1561 aatacggttt gtatttgagc gagatctgct aacgcgatgt cgctacatcc gttggcatcg
     1621 attacccact cggtccccag atttatgttc ataactgaat gtaaagtaat gccgctgata
     1681 gaacgtcagc tgagactcga tttcgcgaga ggataaggca gtgactcgcc aaattcaaat
     1741 tgtgcgagca aaaaaagacc gattagggct tcgggcgttg gttttgactc accggtcgct
     1801 tagttctaaa gaggtcgcct gattcgaaag gcgaatgcca gtacaaagca cacagttgtc
     1861 ggatggcata gtgcgagaga actgcgccgg gtgcccgcgt tgtccccttc aaacgtacgc
     1921 aggcaccctg cgtggcgcta acccctttct cgcaggtaag ggaggctaac gcaatttaat
     1981 ggcgagagtc cgttgtcaca caacggcgta gcgctcttcg caaatgtcta taagttcctg
     2041 tagttgggac ttgtcgagat agaaattcgc gccagcttcg agacaaacat cgcgaagcac
     2101 atcggaagga tcagctgtga acataatgat cacaatttcg ggatcttcgc gtcttatttc
     2161 cttgagagca tcgatgccac tacggtgagg catcgcaatg tctaagacga cgatgtcggg
     2221 tttgagagct cgcaccatgt tgatcgcttc aacgccatca acggccatgc cgacgagcat
     2281 gcaaccttcg acctgggcca acgcacgctt gagatgctct cggacgacag gagaatcatc
     2341 ggcaataacg atcgtcaaat ccttcaaatc tacagcttcc ctccactcgc atggaaaaga
     2401 tgagagtttg agggcggcag gctgtttctc gcaagcgagc gaactatgat ttttgtgtaa
     2461 ggaatttgct caacctcgtg taggtatttg ccttacagaa ggttagtttg actgcttcgg
     2521 tgtggcggaa ttaccctttc gcatcttctc gataacctgt aaattagcta gcccaagatt
     2581 ttcgaaagat ccttcagttg cttttcaggg tcatatgctc gaatcgtgaa acaagcgcgt
     2641 cgaattgata cgaaccagga aaggatggtg aacccttgat ccaacgtctc tcaactctta
     2701 tctgcgcctt ggttgttgct ctagcgatgt gcggcgtcgc taacgcgcaa aaaatagagt
     2761 cactaaatgc taccgccaaa ggtaagggaa ccatcagtac cggcgtcgat aaactaacta
     2821 tctattcggt gatggtcgtg ctgaaggaaa acggcgatgc ggaatttacg ttctattgtg
     2881 atttgcaact gagtgctaag ggtcgctgga ctgcgaacga gtcgtcagat cagggaatca
     2941 atattgaaat taccggcggt gttgtttccg gcaacgcgac aggaactggg aagttgttgc
     3001 ttcgtccaga cggaaaatcg atcgataaat tgaatatcga cgcgaagtct gggagtcgta
     3061 caatttcaat caagtttgtg gctgataagt aaataactca aaaggttatt ctttgatcgc
     3121 ttctgtgagt ttgagtttca gtctctgaat agcctcgtct gtcgttcgca atgaggctag
     3181 cgtccgcatc ttatcttgtc gcaatagttc caattgcatt tgaactcgcc cccgttcatt
     3241 gatgatgcgt cgacgaagct cctcccgagc ttcttctgga tgaactgagc caacgccaga
     3301 catgatgctt tcaatccgtt ctggtttcag gttttgattg agctggtcca ttcgcgcttg
     3361 taatggctct tctttctcgg cgatttcgat cagatgtaat tgaagaccct cgccacgctg
     3421 ttcctctctt gtcagcatgt cctgaatcgt gttgatctgc cgatcttttt ccaactctgc
     3481 cagtcgcttt cggagccgtg gcatttcctg attcagacgt tgcaggtttt ctcgtatttc
     3541 atcgcaactc tgcgacgcac cgctttggga atttgttgtg gattgacttt gggaggctcg
     3601 atgctcgctc ttcgcgtttt gcgagcaaat actaattgcg gttgcggcag ttataacact
     3661 caaaaagttg cagaggttgc gagcaagtgg gtttctcatc aaacacctcg atttccaaat
     3721 gctttctgta cttgcacttt gtcgtgatgt tgtcagtgcc tcatctcgag cgtcagttgc
     3781 ttgcaaacaa agtacaaaac acaaagcgca attgaagctt ggtaagttat gtggttcgtc
     3841 actccccgta gcgagcgact acgactgtaa tgttatcgcg acctccacca tcgagtgcag
     3901 cattaactaa ctcctggcag gcactgtcgc taccaccggt ggaaccgaga atggacgcaa
     3961 tcttatcatc aggaaccatt tcagtgagtc catcggtaca gagcaacacc tgatccccca
     4021 ttttgatgtc gactcgttga acttgaggtt ctgtaccttt tgctattgct ccgagcgcgg
     4081 tggtgagtac gtgtcgagta gtgcgggttg acggatcttc gggtcttata atgccggcgt
     4141 ctatgagagc ctgtgccaat gtgtgatcgc tggtcagttg atgcattttg tcttcgcgca
     4201 tcaggtaagc acgcgaatcg ccaaggtggc ccactaatag gtgacttccg agtgatacgc
     4261 caagagtcaa tgtcgtaccc attcccacca aagacggttc gactttcgta tgggctttga
     4321 tcgcctcgtc aatctgatgg aatcgttgag tgatgcgttg agttaactta gcgacgtcct
     4381 ccggttcgct gaacctcatg agccagtcgg gcgtgttaac gaccagctcc accaatttac
     4441 acaacgccat ccgactcgca atctcgccgc cggccattcc tcccattcca tcagccacga
     4501 ccattccgta accggtctct tcgaaggccg tattcagatg gccgtcttta atattcgtcg
     4561 agagagtctc cagcgatcgc tggaaacgaa gggtcaggta atggtcttcg ttgtttttgc
     4621 gtttgtgtcc ttgatggctg gcgccggcga tatctacgcg gacgcctgat gtgggtagac
     4681 tcagtccggg aaagaaccac tgcggaggta aaagttccag cgtgtcgctg tcgctgcgtg
     4741 aatttgcatc tgtccccatg agaatcactc cagtggcatt acttcaggtt aggctgtgat
     4801 gaaaccaata tcactctcca cctgttagtg caatgatttt ggagcgttca attaaatcac
     4861 ttcgattcct tagttatatc aaagcgagca taaaagatgt ttgtgtcttt atgagtttcg
     4921 tcctgtgcaa ttacgatcgc caggccatcc tctttttggc catccttttt tgaaaaaacg
     4981 cagaatgctc catgattttt cgtatcttct gtgtcgccca cgcagccttt gtcgtttggc
     5041 gtccagccag cgcttttcat tcgctcattt gaatagaagt cttttacttc ctgagcagtc
     5101 ttgtcggttc tgaaagagat gaaatttaac ttacctcgca tagcagcacg gatcgcgagt
     5161 cgcgcaccca atggaatttc caaatcagcc ttcgtggcgc cctgaaaggg tggtacatcc
     5221 ggccataggg aactaacggt tcctccattc ttatcgccag ttaagctttc ggcgagacga
     5281 caagagacac acacggcaaa gagaaggatt aaggcgagta aagaggtgga gagtttcatt
     5341 tggtcctccg gactgtgggg tttgtgttgt acgatcccta ttgcccaact attgctcgga
     5401 cgcgctctct cttaaggagc gttacaatgc cgtcgcgacg ataccagatt ccctctttcg
     5461 cccgccaaac ctgatccgct tcgattctgc caccagttgt taacagaatt atcggcttac
     5521 ccgaggtttt accatgatta ctaacaccac ttgtagaaac tggtccaata ctcaccgccg
     5581 ctcgactatt tgtatttcga gtaggtgtcg cagcaacttt cggtgttggc tcaggcgtcg
     5641 actcaacgat tggagtccgg acattcggta ccgcctgagg cttctctcct gagggtagag
     5701 ttaccgccgt gatctgattc acggctccat tactacttga gcgcgttttt attatcagat
     5761 aaacacccag caggcccaag cctaaaatca acaacaacaa cgcggcgact agaattggaa
     5821 tcagcttttt cgccggagat ttaagagtta cagacggaac cgtctcaggc gagcttactt
     5881 tggatgagtt tgtccttatc gccgcctcaa cagtctcatc gtcgatcgat acgaggagtg
     5941 tctgatcgaa atcacactgc tcgtcagagt ttagataaat gaactggcac tgaggacaac
     6001 gtttcataga tttaattact acgagatcga tcggctaaaa cgatcagtac agaaacggta
     6061 ctgacgtcaa tcatcaacca gtgatagcat ccgcacgcta cacggtagaa ctctcccaaa
     6121 aggagtttga ttatgaagag tgtacggttc acggtttcga tcatattagc ggtcctgttc
     6181 ggagcttgtt gggctaaaaa ggcgccagca cctccggcat cctcaggccc atcaccggcc
     6241 gattcgccac gagtttcaga aacgtcgcag cctccaagtc cgcccatggg tgcgccgaaa
     6301 gctggcccgg gtgaattgca gaccggtcaa gcaagtggca gttacaccgc gggaggtcaa
     6361 acagttgaat tgaagtatgc atatgccgga catgctgtgc gtttcagcac tgaatcattg
     6421 gttatcttgt taactgat
//
LOCUS       DASSFP010000381         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_261190, whole genome shotgun sequence.
ACCESSION   DASSFP010000381 DASSFP010000000
VERSION     DASSFP010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261190"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFH91_08585"
     CDS             <1..447
                     /locus_tag="VFH91_08585"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:115..117,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET6863092.1"
                     /translation="AAPDFTLPSTAGQKVKLSDFKGKKNVVLAFYVLAFTGGUTKELK
                     AYQADIAKFEGTDTQVFGVSVDSVPANRRFAQDIGVTFPLLSDFKRTVVKDYGIFNEE
                     QGFGNRATFVIDKNGIIQHIDEGNVAIDPTAAAETCNVLMHKKATN"
     gene            709..1623
                     /locus_tag="VFH91_08590"
     CDS             709..1623
                     /locus_tag="VFH91_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863093.1"
                     /translation="MKQVRYFALTLGLLCSLSISLAQQPCNPNSLPLQLRDLAANRPI
                     DSCKCTGAPKGEPGSQISIANDLQNVIKNNFAGPADNPKRISIRNMLQLQAKVDSFSM
                     VELPRGDRFTLPSPDQRKLLRNLTIDNGKTFSEGDVVTLAGFMLGARHSNLEDGESVN
                     CEKGGCGNNDVHIELAPRRRDFTMDKAEQMNDEGVVAEISPRHRPESWNRFDSADYRN
                     FFKNHPVAFTGQLFYDASHSPGGAPDRASVWEVHPVYAIWVCRNTTAQACSLDQLNNE
                     GVWLPFHKLKNALHLPTVHVTDRCKENP"
     gene            1667..>2401
                     /locus_tag="VFH91_08595"
     CDS             1667..>2401
                     /locus_tag="VFH91_08595"
                     /inference="COORDINATES: protein motif:HMM:NF023695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HET6863094.1"
                     /translation="MKLHTRNPVLEFNDEPKLRESLSAAVFAEQDLWVASDETTSIER
                     LSTDEGLIFRHHKTFLLEQLITLPAVGTDLDQEVDIEGMDRFDSYLWVIGSHSIKRKK
                     VDSKDPDGDEKLIKKLAKTEDSGNRCLLARIPIIENGSSGELVRSTNPEPSGNPILNA
                     GSLAGDVRSDALTDAIRKAHGGSGDTHLARFLDIPGKDNGFDIEGLAVTTNKAFVGLR
                     GPVLRGWAVILEVSFQVTDEAELMLKD"
BASE COUNT          641 a          618 c          609 g          533 t
ORIGIN      
        1 gctgcaccgg atttcacgct cccaagcact gccggtcaaa aagtaaaatt aagcgatttc
       61 aaaggtaaga aaaatgtagt gcttgccttc tatgtcctgg ccttcaccgg tggttgaacg
      121 aaagaattga aggcgtatca ggctgatatc gctaaattcg aaggaaccga cactcaggtt
      181 tttggtgtca gcgtcgatag cgttcctgcc aatcgccgat ttgcacaaga catcggcgtc
      241 acctttccac tcctcagcga tttcaaacga accgtggtga aagactacgg gatcttcaac
      301 gaagaacagg gcttcggcaa tcgagcgaca tttgtcatcg acaagaatgg catcattcaa
      361 cacatcgatg aaggaaatgt cgcaatcgat ccgaccgccg ccgcggaaac ctgcaacgta
      421 ctaatgcaca agaaggccac taactagttg ccaactttac agcgtgtctc gtggatcgct
      481 ctttctggat tggtgcaatc cacgagctcg ctagcgggaa tgaaacatac gggaagacat
      541 tgactttctt cccttatgag attatgttgc gcccgttcac tcgggcgaat tctcaagggt
      601 cactcggcct caatactcgc aaatctggcc tttaagaaac gactgacctt tagccctcta
      661 aaacccaggc tctttctcaa tcgcttccac tcttaacgga ggacccccat gaagcaagtt
      721 cgttattttg cgctcactct cggactgcta tgctcactca gcatctctct ggctcaacaa
      781 ccttgtaacc caaatagtct cccgctacag ctgcgcgatc tggcagccaa tcgtccgatc
      841 gacagctgta agtgcactgg cgcgccgaaa ggcgaacctg ggagtcaaat cagcatcgcc
      901 aatgatcttc agaacgtcat taagaacaat tttgccgggc cggcggacaa tccaaaacgc
      961 atcagcatta gaaacatgct gcaattgcaa gctaaggtcg acagtttttc gatggtggaa
     1021 ttgccccgcg gcgatcgatt cacactgccc agcccagacc aacgtaagtt gctcaggaac
     1081 ttaaccatcg acaacggcaa gacatttagc gaaggcgacg tggttacact cgccggattc
     1141 atgctgggag ctcgacattc gaacctggag gacggtgaga gtgtgaactg tgaaaaagga
     1201 gggtgcggaa acaatgacgt tcacatcgaa ctcgcccctc gtcgacgtga cttcaccatg
     1261 gacaaagcag aacagatgaa tgacgaaggt gtggtcgcag aaataagtcc gcgtcatcgg
     1321 ccggagtcgt ggaacagatt cgactccgca gactacagga atttcttcaa gaatcatccc
     1381 gttgccttca ccggacaact gttctatgac gctagccata gccctggagg tgcgcctgat
     1441 cgcgcttccg tatgggaggt tcatccggtc tatgcaatct gggtatgcag aaatactacg
     1501 gctcaagcct gttcgttgga ccaactgaac aacgaaggcg tctggttgcc gtttcacaag
     1561 cttaagaatg cactacacct tccgacggtg cacgttacag accgctgtaa ggagaatcca
     1621 tgactcgtca cgttcgtgac gcctgctccc attaaggtat aagacgatga aacttcacac
     1681 tcgtaatcct gtgcttgagt ttaatgacga gccaaagttg agggaatcac tctcggcggc
     1741 cgtcttcgca gagcaagatc tgtgggtcgc gtccgatgag accaccagca ttgaacgact
     1801 ctcaaccgac gagggcctga tttttagaca ccacaaaaca ttcttgttgg aacaactgat
     1861 cacactcccg gccgttggaa cggacctgga ccaggaagta gatattgagg gaatggatcg
     1921 gttcgattca tatctctggg tcataggatc acatagcatc aaaagaaaga aagtcgatag
     1981 caaagacccg gatggcgacg aaaagttaat caagaagctc gcgaagaccg aagacagcgg
     2041 taatcgctgt ctgctggcgc ggataccgat aattgaaaac ggttcgtctg gcgaacttgt
     2101 cagatcgacc aatcccgaac cgtcgggaaa ccccattctg aacgcgggaa gtcttgcggg
     2161 tgacgtcagg agtgacgcgc taaccgatgc gataagaaag gcgcacggcg gaagtgggga
     2221 tactcacctt gctcgattcc tggacatacc tggcaaggat aatggatttg acatagaggg
     2281 gctggctgtc accacaaaca aagcttttgt tgggcttcgt ggtccagtgc tacgcggttg
     2341 ggcagtgatt ctcgaggtgt cttttcaagt cactgatgaa gcggagctca tgctaaagga
     2401 c
//
LOCUS       DASSFP010000382         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_107525, whole genome shotgun sequence.
ACCESSION   DASSFP010000382 DASSFP010000000
VERSION     DASSFP010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107525"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /gene="argH"
                     /locus_tag="VFH91_08600"
     CDS             complement(<1..1021)
                     /gene="argH"
                     /locus_tag="VFH91_08600"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET6863095.1"
                     /translation="MAETKNLWGGRFKGKTDPGFAAFNNSFRFDRRMFDQDVDASIAY
                     AKALQLVGVLTTSEAQAIQDGLREIKKRAEDDESYFDADSEDVHSFVESRLIELVGDV
                     GRKLHTGRSRNDQVATDFRLWVREAIDRLDEEVRNSQSALLDFAESNKEIVIPGYTHL
                     QPAQPVLLAHWCLAYFEMLARDRSRLSDTRRRVNVMPLGSAALAGNSFGIDREALAAD
                     LGFADVSKNSLDAVSDRDFCLEFLSNCSLVMVHLSRLAEDIVIYSTNEFGLIQLGDAI
                     ATGSSLMPQKKNPDSMELVRGKAGRVFGDLAGMLATMKGLPLAYNKDMQEDKEAVFDA
                     FDTVTA"
     gene            1257..1670
                     /locus_tag="VFH91_08605"
     CDS             1257..1670
                     /locus_tag="VFH91_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863096.1"
                     /translation="MNKQERQQKILGLIRATPIGTQEDLRALLEKSGVPATQSSVSRD
                     LEELGVVKHHGHYTLPQPNGSTARGLLSLDHAGDSLVIARTVPGLASAVAVEIDAASI
                     PEIVGTIAGEDTVFIAVRDQKSQRSTMKRVLELFG"
     gene            1690..2886
                     /locus_tag="VFH91_08610"
     CDS             1690..2886
                     /locus_tag="VFH91_08610"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET6863097.1"
                     /translation="MQKKINKIVLAYSGGLDTSVMLHWLKEHYHCEVVCYCADVGQGA
                     ELSGLEEKAHATGASKLYVEDLREEFVRDYVWTALKANAVYEGVYLMGTSLARPVIAK
                     RQIEIAQQEGADAVAHGATGKGNDQVRFELTYYALQPDIKVIAPWREWDFKGRMDLIA
                     YAREHNIPVTATAEKPYSTDGNLMHISYEGGILEDPWAGPPEDIFQLTKSPESAKPEA
                     EYVELSFEKGEPVAINGKRLDPVQLLSDLNNLGGEHGIGRVDLVENRFVGMKSRGVYE
                     TPGVTILQAAHRALESITLDREVMHLRDSLGVKFAESIYYGFWFAPEFKLMRKMVEET
                     QQRVSGDVRLKLYRGNAIVVGRRSPNSLYDEKIATFEADTVYNQRDAEGFIKLNALRL
                     RLGQQS"
BASE COUNT          760 a          797 c          786 g          701 t
ORIGIN      
        1 aggcggtaac agtatcgaat gcgtcgaaca ccgcctcttt gtcttcttgc atgtccttat
       61 tgtaagcaag cggcaacccc ttcatcgttg ccagcattcc cgcgaggtcg ccaaacactc
      121 gacccgcttt cccgcggacc agctccatcg agtcagggtt ctttttctgc ggcatcaaac
      181 tcgatccggt cgctatcgca tcaccaagtt gaatcagacc aaactcgttc gtggaataaa
      241 taacgatgtc ttccgccagc ctggagagat ggaccatcac gagcgagcaa ttcgacagga
      301 actcgagaca aaaatctcta tcactcaccg cgtcgagact gttctttgac acatctgcaa
      361 acccaagatc cgccgcgagt gcttctcgat ctatcccaaa cgagtttcct gcgagcgcgg
      421 ctgaacccag gggcatcacg tttactcgcc ggcgagtatc ggatagacgc gagcgatcgc
      481 gtgcgagcat ctcaaagtag gcgaggcacc aatgggccaa caggactggt tgggccggtt
      541 gtaaatgagt gtagccaggt atgacgattt ccttatttga ctctgcgaaa tcaagtaggg
      601 cggattgaga atttcgaact tcttcatcca accgatcaat cgcttcacgc acccagagcc
      661 gaaagtcggt cgccacctga tcgttgcgac tgcgtccagt gtggagtttc ctgccgacat
      721 cgccgacaag ctcaatcaat cgactctcaa cgaacgaatg aacgtcttca gaatctgcgt
      781 cgaaatatga ctcgtcatct tcggcccgtt tcttgatttc ccgcaatcca tcctggatcg
      841 cttgagcttc actcgtagtt agaactccca ccaactggag tgcttttgcg taggcgatgc
      901 tcgcgtcgac atcctggtca aacattctgc ggtcaaaccg gaaagaatta ttgaaggccg
      961 cgaaccccgg atcagtcttg cccttgaacc ttccacccca aagatttttc gtctcagcca
     1021 tcaattccct cccctggtgt cctaatctga accttctctg tgcaaccttt gtgtcctctg
     1081 ttgctcgtag tgattggttt tcttaggact ctgctcacca ccgaggcaca gaggacacaa
     1141 aggttgcaca gagtaagcca aattaggact ccgcctacaa atttgaccct cgcacaactc
     1201 ccgtggtagt ttatgcatct atgcattgtt tatgcaagag aagcgcataa attactttga
     1261 ataagcaaga acgacaacaa aagatcctgg gcttaattcg agcgacgccg attggcacgc
     1321 aggaagattt gcgtgcgcta ctcgaaaagt cgggcgtgcc ggcgacgcaa tcgagtgtgt
     1381 ccagagatct ggaggaactt ggtgtcgtca aacatcacgg tcattacacg ctgccccagc
     1441 caaatggatc aactgctcga gggctgctga gtttagacca tgctggagat tcactggtaa
     1501 ttgccagaac tgttccgggt ctggcgtctg ctgtggctgt ggagattgac gctgcatcga
     1561 ttccggaaat tgtagggacg atcgcaggtg aggataccgt ctttatcgcc gtccgagatc
     1621 agaaatcaca acggtcaacg atgaagaggg ttttggagtt atttggatga gaggaaaaca
     1681 aagaacctgg tgcagaagaa aatcaacaaa atagtcttag cgtactcggg tgggctcgac
     1741 acatcagtca tgcttcactg gttgaaagaa cactaccact gtgaagtcgt ttgttactgc
     1801 gctgatgttg gccagggtgc agaactctct ggtcttgaag aaaaggccca cgctaccggt
     1861 gcttcaaaac tttacgtcga ggatctacgc gaagagtttg ttcgcgacta cgtatggacc
     1921 gcgctcaagg cgaacgctgt ctacgaaggc gtctacttga tgggcacttc cctggcccga
     1981 cccgtcatcg ccaagcgtca gatcgaaatt gctcagcagg aaggcgccga tgcggtggcc
     2041 catggcgcaa ccggtaaagg caacgatcag gttcgctttg aattgaccta ttacgccttg
     2101 cagccggata tcaaggttat tgcgccctgg cgcgagtggg acttcaaagg ccgaatggat
     2161 ctgatcgctt atgctcgcga gcataacatt cctgtgaccg ccacagctga gaagccttat
     2221 tcaaccgacg gcaacctgat gcacatttct tatgaaggcg ggatcctgga agatccctgg
     2281 gcgggaccgc cggaggatat tttccaatta accaaatccc cggagagtgc gaaacctgag
     2341 gctgagtatg tcgaattgtc tttcgagaag ggtgaacctg tcgcgataaa cggcaaacgc
     2401 ttagacccgg tacagctcct ttctgattta aacaacctcg gcggcgaaca cggaattgga
     2461 cgtgtcgatc tggtcgaaaa tcgatttgta ggtatgaagt cgcgcggcgt ctatgaaact
     2521 ccgggggtca cgattcttca agccgcacac cgagcactcg agtccataac actcgaccgc
     2581 gaggtgatgc acctgcgtga ttcactcggc gttaagttcg cagaaagcat ctattacggg
     2641 ttctggttcg ctcccgagtt caaattgatg cgaaagatgg tcgaggagac gcagcaacgg
     2701 gtgtcaggag atgttcgact caagttgtat cgaggcaatg ccattgtcgt aggtagacgg
     2761 tcacccaatt cgttgtacga cgagaagatc gcaacgtttg aggctgacac ggtttacaac
     2821 cagcgcgacg cggaaggctt tataaaactg aacgccttga gactaagact cggacaacaa
     2881 agctgacggg ttggaggaca acagatgaac ttgcagaatc aaattacagt taccgacgtc
     2941 gacaaaatcg gttccgcaac ttcgccactc ggattaacaa gaacagtggc ctatgttcac
     3001 gcatccacta acccgcaggc cggggatgtt gtcgtggtcc gagc
//
LOCUS       DASSFP010000383         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1304792, whole genome shotgun sequence.
ACCESSION   DASSFP010000383 DASSFP010000000
VERSION     DASSFP010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304792"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="VFH91_08615"
     CDS             <1..1759
                     /locus_tag="VFH91_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6863098.1"
                     /translation="TVAQAQTQLNTIAARLAQTYPETNLGTLERPNEPRPITIVSVSR
                     LDPSAQKGILSISLLLFAVVGIVLIIACANVANLMLARASGRRREIAIRLAVGASRRQ
                     LIRQLLTESLMLSGLGGLIGFIAAQWAAGSLPSFFPANEIEGLNLTLDWRVLLFTIGI
                     SFVTGLLFGLVPAIHSTRMNLVPSLKDEATATIQRFGRVGLRDVLVVTQLALSLVLLV
                     GAALFVRSLKQALTYDPGFASQSLLIASLETRGTGIDKQQGRLFYEQMLDRVRHLPGV
                     QGATLTRVIPISGGGQRRGINLEGYQPRENEDTELNTNVVGPDFFSTMGIPLVQGREF
                     SAQDREGSPGVVIVNEELSQRYFGGNALGKRLRMGSDNPYLEIIGIARNAKYRNLREQ
                     TLPFIYIPLAQEYQPGMTVMVRTAGDPVSQLAPLRNEMRSLNRDVPVFSVQTMTDRIG
                     GQLAGDRLIAVLLSIFGIAALMLASIGIYGVVRYSVSQRTHEIGIRMALGAERGHILK
                     LILRQGMSLILFGVGVGLGISLLLTRVLKNLLFGISATDPITFAVVVLVLGGVALLAC
                     YFPARRATKVDPLVALRYE"
     gene            1809..>2915
                     /locus_tag="VFH91_08620"
     CDS             1809..>2915
                     /locus_tag="VFH91_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6863099.1"
                     /translation="MNQLIQDIRYGLRGLLKRPGFTAVAVLTLALGIGANAAIFSVVN
                     AVLLKPLQFRDPQQLVMVWEDAAMIGFPQNTPAPANYIDWKNQSQSFQDMAAHVETSF
                     NLTGNGEPERVAARAITSNFLPLLGIQPMLGRSFNEEETKPGLDHVAILSYPLWQSRF
                     GGDRSVLNKEIQLNGEKYTVVGVMPAGFQFIDPEVRMWIPLAFTPEDLANRNGHYLTV
                     VGRLKSGVNLASAQADMNAVMRRITQDHPAETINGQLGVLVIPLHDQLAGDSKKSLVV
                     LLVAVAFVLLIACANIASLLLARAAGRRREIAVRTALGASRSRIVKQLLTESLLLSVF
                     GGLLGLAFAFGSFGFFRQLIPEGLALSTDLRLDGS"
BASE COUNT          673 a          708 c          773 g          761 t
ORIGIN      
        1 aacagtggcc caggcgcaga ctcaacttaa caccattgcg gcgcgactgg ctcagactta
       61 tccggaaaca aatctcggga cacttgaacg acccaacgag ccaagaccta ttacgatcgt
      121 aagcgtttct cgattagatc catcagcgca gaaaggaata ttgagcatct cgttattgct
      181 ttttgcggta gtgggaattg ttctaatcat tgcgtgcgct aacgtggcca atctaatgct
      241 tgcgcgcgca tctggacgcc ggcgagagat cgcaatacgt cttgccgtcg gtgcaagtcg
      301 tcgccaatta atcagacaat tgctcaccga aagtctgatg ctctccggac tcggcggact
      361 gattggtttt attgcggctc aatgggcggc ggggtcattg ccttcattct tccccgcaaa
      421 cgaaatagag ggattaaacc tgacgctcga ctggcgagtg ttgttgttta ccatcggcat
      481 tagttttgtg accggtctcc tttttggtct cgtgcctgca attcattcga cgagaatgaa
      541 cctagtaccc tcattgaaag atgaagctac agcaacaata cagcgtttcg gccgtgtagg
      601 actgcgcgat gttctcgtcg tgacgcaact ggcattatcg ctggtcctgt tagttggcgc
      661 ggcacttttt gtccggagtc tgaaacaagc gctcacctac gatcccggct ttgcttctca
      721 gagtttgctg atcgcctcac ttgaaacacg cggcacaggg atcgacaagc aacagggccg
      781 gttgttttat gaacaaatgc ttgatcgcgt caggcacttg cccggcgttc aaggcgcaac
      841 tctaacccgc gtgattccta tcagcggtgg tggtcagcga cgcggtatta atctggaagg
      901 ctatcaaccg cgagaaaacg aagatactga actcaataca aatgttgttg gtccagactt
      961 cttctcgact atgggaattc cgctggttca gggtcgtgag tttagtgcgc aggatcggga
     1021 aggatctccg ggagttgtga tcgttaatga agaactgtca caacgttact ttggaggcaa
     1081 cgcgcttggt aaacgactgc ggatgggctc cgataatccg taccttgaaa tcatcggtat
     1141 agctcgaaac gcgaagtaca ggaatttaag agagcaaact ctgccgttca tctacattcc
     1201 cctggcacag gaatatcaac caggcatgac ggtgatggtt cggactgcgg gcgatccagt
     1261 ttcgcaatta gcccccttgc gcaacgagat gagatcgctt aatcgggatg tccccgtgtt
     1321 ctctgttcaa acgatgacgg atcgaatcgg tggccagttg gctggggatc ggttaatcgc
     1381 agttttactg agcatctttg gaatagccgc tttaatgctg gcttcgattg ggatctatgg
     1441 agtcgtcagg tattccgtgt cacagaggac acacgagatc ggaatccgga tggcgttggg
     1501 agcagagcgc ggccacattt tgaaactgat cctgcgacaa ggcatgtctc tcatactgtt
     1561 cggagtcggc gttggtctag gtatctcgtt gttgttgacg agagtgttga agaatttgct
     1621 cttcggtatc agcgcgaccg acccgattac atttgcagtc gtagttctgg tgttgggagg
     1681 cgtcgccctc ctcgcttgtt attttccggc tcggcgcgca accaaagttg atcctctggt
     1741 tgctcttcgt tacgagtgaa gtcgtttgca ttcttgaata tttagtttga agaaagatct
     1801 ttaagaccat gaatcagctc atacaagaca tacgttacgg gctaagaggt ttgcttaagc
     1861 gacctggttt taccgcagtc gccgtattga cgctcgccct tggaattggc gctaacgctg
     1921 caatcttcag cgtcgtcaac gctgtcttac tcaagccact tcaatttcga gatccgcagc
     1981 agttagtgat ggtctgggaa gatgcggcga tgatcgggtt ccctcaaaac actccggcgc
     2041 cggctaatta catcgattgg aagaaccagt cacagtcatt ccaggacatg gcagcgcacg
     2101 ttgaaaccag tttcaatttg acggggaatg gtgaacctga acgtgtcgcc gctcgagcga
     2161 taacctcaaa tttcttaccg ctgcttggga tccagccaat gttgggaaga tcgtttaatg
     2221 aggaggaaac caagcctggc ctggaccatg tggccatact tagttatcca ctctggcagt
     2281 cgagatttgg cggcgatcga agtgtattga ataaggaaat acagcttaat ggcgagaaat
     2341 acactgttgt cggagtcatg cccgctggtt ttcaatttat cgatcccgaa gtgcgcatgt
     2401 ggatcccgtt ggcatttact cccgaggatc tcgctaacag gaatggacac tatctaacag
     2461 tagtcggtcg tctcaaatcc ggcgtcaatt tggcaagcgc tcaggctgat atgaacgcag
     2521 tgatgcgaag aatcactcaa gatcatccgg ccgaaacgat taatggtcag ctgggagtcc
     2581 tcgtaatccc tctgcatgat cagctggccg gcgattcaaa gaaatcgtta gtggtcctgc
     2641 tcgtggcggt agcgtttgta ttgctcatcg cttgtgcaaa catcgccagc ctcttactcg
     2701 cgagagcggc gggacgacgt cgtgaaattg cggtcagaac ggcactcggc gccagccggt
     2761 ctcgcattgt caaacagctg ttaaccgaaa gtcttctcct ttcagttttc ggaggtttac
     2821 ttggcttagc attcgcattt ggaagctttg gattctttcg ccaactaatt cctgaaggat
     2881 tagctctctc aactgatctt aggctcgacg gttca
//
LOCUS       DASSFP010000384         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_108449, whole genome shotgun sequence.
ACCESSION   DASSFP010000384 DASSFP010000000
VERSION     DASSFP010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108449"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..1356
                     /locus_tag="VFH91_08625"
     CDS             358..1356
                     /locus_tag="VFH91_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6863100.1"
                     /translation="MRKLLPLLLLLALCGLSGCTQQNGSNGKKKFTIAVIPKGTTHEF
                     WKSIHAGSNKAAGEMSALGIDTEIIWNGPLREDDREEQIKVVEGFAAQGISGIVLAPL
                     DNRALVRPVVDAGSAGVPTVIIDSGLETDNIVSFVATDNRKGGVLAADRMGQLLNGKG
                     KVIVLRYAEGSASTTEREEGFILEIKQKFPEIQLISTDQYAGATRDTAKRASENLLNR
                     FGDEVQGIFTPNESSTAGMLSALQDIGKAGKLKFVGFDNSQLFIDAMRADQLHGIVVQ
                     NPFNMGYLGVKTIVDHLLGKPVEKKIDTGVMLVMKENLDAPEVQTLLHPPLDQYLK"
     gene            1369..2370
                     /locus_tag="VFH91_08630"
     CDS             1369..2370
                     /locus_tag="VFH91_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6863101.1"
                     /translation="MVADVKPDRSVFNSSSLNEWLNRLGPLLGLVLVIGIFSVLMDTP
                     SRYLSANNLRIVFAQTVIVAIGAIGMTLIIISGGIDLAVGSTIALTGVITALSVNAGL
                     PPISALAIGVLVGGVVGVVNGLAITRLKVVPFIATLGMLGVARGTAKWLANQQTVNVP
                     DTWLNELLVMFPKPSWLLVAPGVWIAIILAVCAAIVLRNTVFGRRVFALGSNESAARA
                     CGIATDRLKVWIYGLAGLLFGLAGVMQMSRLRQGDPTVAAGAELDIIAAVVIGGGSLS
                     GGEGSILGSMIGALIMAFLRNGCQQVGWPNYIQEIIIGAIIVIAVAIDRWRSARVVA"
     gene            2496..3035
                     /locus_tag="VFH91_08635"
     CDS             2496..3035
                     /locus_tag="VFH91_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863102.1"
                     /translation="MSVTTGRLLASLFCLVISSIVSLGQNSTANKEPSKPIRPFVVMT
                     AQSLSDLEKKLQPSNKVEELIGGPGMELRVAVQHEQNIASAMAELHDASDDVYYVLSG
                     SATLTLGGTLDTPKEVEPGEWRGAKISGGQQVQIKQGDLVVVPRGTPHQRSTVDQNFN
                     MILIKVFADPRPAAPVKKP"
     gene            3049..>3760
                     /locus_tag="VFH91_08640"
     CDS             3049..>3760
                     /locus_tag="VFH91_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6863103.1"
                     /translation="MIRLFLLCAILISTYVSMMGQTSTSSTSTCDEYKTRAERAESRL
                     NDWPALSRYAADNSKVVPPVKSEQRVVFMGDSITDSWDSPSYGGFFPGKPYVNRGISG
                     QTTPQMLIRFRPDVIDLKPKVVVILAGTNDIAGNTGPMSLETIEGNLVSMAELARQNG
                     IKVVLSSLLPVSDYESRDGKPITQTVRRPPDKILALNKWIKAYADAHRLIYLDYFSAM
                     VDDKGFLKDELSEDGLHPN"
BASE COUNT          951 a          858 c         1010 g          941 t
ORIGIN      
        1 gacgagatgg tagttgagat tgagggaatc agtggcctta acaactccgt agttggacca
       61 aagtaaacct ttggagtgcg gcggcccggc gccgcttttt cgaatctcgt tttacgagcg
      121 actagttaga aatagaacca ccataaataa agggacctaa agagcggcgc ttgtttttcg
      181 aatctcgttt tacgagcgac gagttagaag tagaagggcc atataaataa aagggaccta
      241 aaaagcagcg cctatttttc gagcctcgtt ttacgagcga caatttagaa tagaaggctc
      301 atagaataga ggggcctaaa aagcggcgcc gggccgccgc actccaaaag gaaaagaatg
      361 cgaaagttac ttccattatt actgctgctt gctctttgtg gcctttcggg ctgcacacaa
      421 caaaatggct caaatggtaa aaagaaattc accatagccg tgattccgaa gggcaccact
      481 catgagttct ggaagagtat tcatgccgga agtaataagg cggcgggcga aatgtcagct
      541 ctgggtatcg acaccgagat aatttggaat ggacctctga gagaagacga tcgagaggaa
      601 cagataaagg tggtagaagg ttttgctgcg cagggcatta gtggaattgt ccttgctcct
      661 ctggataatc gtgcactcgt ccgtccggtt gttgatgctg ggagcgcagg agttccgact
      721 gtcataattg attcgggact tgaaacggat aatatcgtca gttttgtggc cactgataat
      781 cgtaagggtg gcgtactcgc agctgaccgc atgggccaat tactaaacgg caagggcaaa
      841 gtgatagtac ttcgatatgc ggaaggatca gccagtacta cggaacgtga ggaaggtttc
      901 attcttgaga taaagcaaaa gtttcctgaa atccaattga tctcaacgga ccagtatgct
      961 ggcgcgaccc gcgataccgc taagcgagcg tcggaaaatc tgctgaatag attcggtgat
     1021 gaagtccagg gaatttttac tcccaatgaa tcctcgactg ccggtatgtt gtctgccctc
     1081 caggatattg ggaaagccgg taaactcaag tttgtgggct ttgataacag ccagttgttt
     1141 atcgacgcca tgcgggctga tcaattacac ggaattgtcg tgcaaaatcc attcaatatg
     1201 ggttatctcg gcgttaaaac aatagtggac cacttgctgg gtaaaccagt agagaaaaaa
     1261 attgatactg gtgtaatgct tgtaatgaag gagaatcttg acgctcctga agtacaaacg
     1321 ctactccacc caccgctgga tcagtatctc aagtagctga acacctgaat ggttgccgac
     1381 gtaaagccgg atcgatcagt ttttaactcc tcaagtctca atgagtggtt gaacaggctt
     1441 ggtccgttac tgggcctcgt cctggtgatc gggatctttt ctgtcctgat ggatacaccg
     1501 agtcggtacc tctccgcaaa taacctccgc atcgtttttg cccaaaccgt gatagttgcg
     1561 attggcgcga tcggaatgac actgatcatt atcagcggag gaatcgatct tgctgttggc
     1621 tccaccatcg ccttaacggg tgtgattacg gccttatcag taaacgctgg cctgcctccg
     1681 atcagtgcgt tggccattgg agtgttggtt ggcggtgtcg tcggagttgt aaatggtctg
     1741 gcgatcactc gattgaaagt agttccgttt atcgcgactc tgggaatgct cggtgtcgcg
     1801 cgaggaactg cgaaatggct tgctaatcaa caaaccgtaa acgttcccga cacctggcta
     1861 aatgagttgc tggtgatgtt tcccaaacct tcatggctgt tggtggcgcc aggcgtgtgg
     1921 attgcgatca ttctcgcagt gtgcgccgct atcgtgttgc gaaacaccgt gtttggccgc
     1981 cgtgtttttg cgcttggctc taacgaaagt gccgcgcgtg cctgtggtat tgcgacggat
     2041 cgtttaaagg tttggattta cggactagcc ggactcctgt ttggcttggc gggtgttatg
     2101 caaatgtccc gtttgcgcca gggtgatccg accgttgccg ctggagctga attggacatc
     2161 atcgcggcag tcgtgattgg tggaggcagc ttaagcggtg gcgagggaag tattctggga
     2221 tcgatgattg gggctctgat aatggctttc ctgcggaatg gttgtcagca ggttggttgg
     2281 cctaattaca tccaggagat aatcatcggt gcaatcatcg taattgcagt ggcgatcgat
     2341 cgttggcgga gcgctcgtgt cgtggcctga atcaggtgcc tgatgacttc gggcggtacg
     2401 aaacgatgac tcattacgcc gtcggcggta ttgatgatga gcgctcatca gagggctctt
     2461 cggttacaag tccatccgaa aattggagca aacatatgtc tgttactacc ggaaggttat
     2521 tggcaagtct tttctgttta gtgattagct cgatcgtgtc tctgggccaa aattccaccg
     2581 caaataaaga gccctctaaa ccaatcagac cctttgttgt gatgactgcg cagtcacttt
     2641 ccgatcttga gaaaaagtta caaccctcaa acaaggtcga agagctgatc ggtgggccag
     2701 gaatggaact gcgcgtggca gtgcagcacg aacagaacat agcctctgca atggctgagc
     2761 ttcatgatgc ttcagacgat gtctattacg tactatcagg ttcggcgaca ctaaccctcg
     2821 gagggacgct cgatacacct aaagaggtcg aacccggtga atggcgtgga gcgaaaatct
     2881 ctggcggtca gcaggtccag atcaaacaag gggaccttgt ggttgttccc agaggtacac
     2941 ctcatcaaag aagtaccgtg gaccagaatt tcaatatgat tctgattaag gtcttcgccg
     3001 atccacgtcc ggcggcgccc gtcaaaaaac cttaggaggc ttactcagat gattcgatta
     3061 tttcttctgt gcgcaatatt gataagtacc tacgtatcga tgatgggcca gacctcgaca
     3121 tcatcaacgt ccacctgtga cgagtacaaa acgagagctg aacgagccga aagccgactg
     3181 aacgactggc ctgcactctc tcgttacgct gctgataatt cgaaggttgt gccacccgtc
     3241 aagagtgagc aacgcgtagt gttcatgggc gattcaatta ctgactcgtg ggattcacca
     3301 tcttacggcg gattcttccc cggtaagcca tacgttaatc gcggaatcag tggacaaacc
     3361 acgccgcaaa tgctcattcg ctttcgtcca gacgtcatag atttgaaacc taaagtggtg
     3421 gttatactcg ccggcactaa cgacattgca ggaaatactg gtccaatgtc tctggaaact
     3481 attgaaggta atctcgtctc aatggctgag ttggctcgcc agaacggcat caaagtggtg
     3541 ctgtcgtcgc tcttacccgt aagcgattat gaaagtaggg acggtaagcc aatcactcaa
     3601 actgttcgac gaccacccga caagattctc gccctcaaca agtggattaa ggcttatgcc
     3661 gacgcgcaca ggctgatcta tctcgactac ttttcagcaa tggtggatga caaaggtttc
     3721 ttgaaagacg aactcagtga agatggctta catcctaacg
//
LOCUS       DASSFP010000385         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1444166, whole genome shotgun sequence.
ACCESSION   DASSFP010000385 DASSFP010000000
VERSION     DASSFP010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444166"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFH91_08645"
     CDS             <1..577
                     /locus_tag="VFH91_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6863104.1"
                     /translation="NQMIIVTPEGNNGWYTDSATNQTEKYESYIINELIADVDSRYRT
                     IRDRRGRAIAGNSMGGYGALKFGLKHPEFFKLAASMSGAFDTPTRTDDKSIMAVFGDA
                     GSSVRQENDIERLARGVNPDKMASLPYFYLDCGLDDPWLGANNHFADVLSETKIPYEY
                     RRLPGGHVWPYWDKQVREVLNVAANVLSPPE"
     gene            610..1194
                     /locus_tag="VFH91_08650"
     CDS             610..1194
                     /locus_tag="VFH91_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6863105.1"
                     /translation="MAKEPPQKHEISQLLDAWSSGNQSALDELYPLVYDELHRLASRY
                     MSRERKGHTLQTTALINEAYVRLVDQRNIHWANRSHFFAISAQIMRRILIDHARRHAY
                     AKRGGGQHPVTLDETATLQVERAAEMIRLDDALQSLAEIDPRRSRVVELRYFGGLSNE
                     EIAETLKISPNTVTRDWNMARAWLYQELAGSEKG"
     gene            1191..3086
                     /locus_tag="VFH91_08655"
     CDS             1191..3086
                     /locus_tag="VFH91_08655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6863106.1"
                     /translation="MKPERWQEISKIFEAAKSLNEPARAVYLQEECGADDLLRSEVER
                     LIESHKKAEAESFISRPAVDEAAIVLNSNADEEQSDSLDENQQFGSYLIQKKLGAGGM
                     GEVYLARDQRLDRTVALKILPVSVANDKRRMQRFRQEARVASSLNQPNILTIYEFGEI
                     DSLSFIATEFVDGKTLRDYLRGKRLKLSEILDISIQIMAALDAAHEAKIVHRDMKPEN
                     VMLRRRDHVVKVLDFGLAKLTETRGGTATREETDAEAATEFKSMPGSLMGTINYMSPE
                     QAQAHVVDERTDLWSVGVMIYEMVAGVMPFKGATSAHTVVQILEKEPSPLTQLDATLP
                     AELQRIVSKAMSKSFDERYQTAKDMMIDLRLLRKQLDATSEINRSAIPPMVSRATAAE
                     RPAITNGAVAVTSRTRRFSPAFIVVPLIAVVIGLAAFFLLLNRTRPQPQLSAPPVTER
                     TLTYSITVQKYRNNQPFEQPFQVAREMLFEPGYQLRLNIDSQQSGFLYVINEGPHEKD
                     KAPEFVLLFPSPKSNRGSALVGENLKIQIPEKSWFSLDNDRGTERVWLVFAPAAIKEL
                     EPARDYANDKTRGLITDAGLRDSIKAFLDSHSLSKPMIEQSDSRKETIVSAHGELLVY
                     AIELEHQ"
     gene            3453..>3795
                     /locus_tag="VFH91_08660"
     CDS             3453..>3795
                     /locus_tag="VFH91_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863107.1"
                     /translation="MPTKWTISANDVSGGSSGRDLVGCHITIVGGIVYQFTAPNNDEL
                     ATSPGPTLPNLPYTFPTVQNYKNHNWTIKLRTLTYGPSNNQAQGNWSNDAAGITAGEG
                     GDWTAQAGATID"
BASE COUNT         1032 a          933 c          977 g          853 t
ORIGIN      
        1 aaaccaaatg atcatcgtaa ctcccgaggg aaataatggt tggtataccg acagtgcgac
       61 gaatcagacg gagaagtacg aaagctatat aatcaatgag ctgatagcag acgtagatag
      121 tcgatataga actatcagag atcgtcgggg tcgcgcgatt gccggcaatt caatgggtgg
      181 atacggagca cttaagttcg gtctcaagca cccggagttt tttaaactag cagcgtcgat
      241 gagcggtgcg tttgatacgc ctactcgtac cgatgacaag tcaataatgg cggtttttgg
      301 ggacgctggt agttctgtac gccaggaaaa cgatatcgaa cgactcgccc gaggggtcaa
      361 ccctgacaag atggcgtcgt tgccctattt ctatctcgat tgtggtctcg acgatccatg
      421 gctgggcgcg aacaatcatt ttgccgacgt gctttctgag acaaaaatac cttacgaata
      481 caggcgactg cctggagggc atgtctggcc ctactgggac aagcaagtac gagaggttct
      541 gaacgttgct gcgaatgtgt tgtcgccacc cgagtagtaa tggtccaaat ctcaaaggac
      601 cgctaacgaa tggccaaaga gccgccccag aaacacgaaa ttagccagct gctcgacgcg
      661 tggagtagtg ggaatcagtc cgcgctcgac gagctatacc cgctcgtgta cgacgaactt
      721 caccgtctcg ctagtcgtta catgagtcgg gaaagaaaag gccatacgct gcagaccaca
      781 gcactcatca atgaagccta tgtacgactt gtcgatcagc gtaatattca ctgggctaac
      841 cggtcacact tcttcgcaat ctcagcgcag ataatgagac gaatcttaat tgatcacgcc
      901 cggcggcatg cttacgcaaa acgaggcggc ggacaacatc cagtcacact cgatgagacc
      961 gcaaccctgc aagttgaaag agccgcagaa atgatcagat tggacgacgc gctgcaaagt
     1021 cttgccgaga tagatccgcg tcggagtcgt gtagttgaac tgcgctactt tggaggcttg
     1081 agtaacgaag aaattgccga gacactcaag atctcgccaa acacagtgac tcgcgactgg
     1141 aatatggcac gagcgtggct ttatcaagaa cttgccggga gtgagaaggg ttgaaacctg
     1201 aacgttggca agaaatcagt aaaatcttcg aagcggccaa gtcgctgaac gaaccggccc
     1261 gcgcagttta ccttcaagaa gaatgcggtg cggatgacct gctcaggtca gaagtcgaac
     1321 gactaatcga aagccacaag aaggccgaag ctgaaagctt catcagtcga ccagcagttg
     1381 atgaagcagc gattgtcctg aattcaaatg cggacgaaga acagtctgat agtctcgacg
     1441 agaatcaaca gtttgggtcg tacctgattc agaaaaaatt gggagcaggc ggtatgggcg
     1501 aggtctatct ggccagggac cagagattgg accgcaccgt tgcccttaag atccttcctg
     1561 tcagcgtcgc gaatgacaag cggcgtatgc aacgatttcg ccaggaggcc cgcgttgctt
     1621 catcattaaa ccaaccaaac atcctcacaa tttacgagtt tggcgaaatc gattctttat
     1681 cttttattgc gaccgaattt gtcgacggga aaacactgcg cgactacctg cgaggcaagc
     1741 gactgaagct ttcagagatc cttgatatca gtattcaaat tatggcagct ctggatgctg
     1801 cgcatgaagc gaagatagtt caccgtgata tgaagccgga gaacgtcatg ctccgccgtc
     1861 gtgatcacgt agtaaaggtg ctcgactttg gactggcgaa gttgactgaa acgcgcggag
     1921 gaacggcaac tcgcgaggaa actgatgcag aggccgccac tgaattcaag agcatgcccg
     1981 gaagtttgat gggaactatc aactatatgt cgcctgagca agctcaagcg catgtcgttg
     2041 acgagcgcac agatctatgg agcgtggggg tgatgatcta cgagatggtt gccggcgtga
     2101 tgcctttcaa aggagctacg agcgctcaca ccgtggttca gatccttgaa aaggaaccat
     2161 ctcctctgac tcagctggat gcaacacttc cggccgaact acagcgcatc gttagtaagg
     2221 ccatgtccaa gagtttcgac gaacgttacc aaacggcgaa ggacatgatg atcgacctgc
     2281 ggctacttag gaagcagctc gatgcgacca gcgaaatcaa tcggtcagca atcccaccga
     2341 tggtaagtcg agcgaccgca gcggaaagac ctgcaatcac gaatggcgcg gttgcagtga
     2401 cctctcgaac caggcggttt agtcctgcct ttatcgtcgt gccattgatt gcagtagtga
     2461 tcggtcttgc ggcgtttttc cttctcctga atcgaacgcg accgcagcct caactttctg
     2521 cacctccggt taccgaaagg acgctgacat attcaataac ggtccagaag tatcggaaca
     2581 atcagccgtt cgagcagccg tttcaagtcg caagggagat gctgttcgaa cccggctatc
     2641 agctcaggtt aaacatcgat agtcaacaat ccggatttct ttatgtgatc aatgaaggac
     2701 ctcacgaaaa agataaagcg ccagagtttg tcttgttgtt tccctcaccg aaatcgaatc
     2761 gcggttctgc cctggtgggt gagaacctga aaattcaaat acctgagaag agttggttta
     2821 gtttggacaa tgacagagga acggaaagag tttggcttgt ttttgctcca gccgctatta
     2881 aggaacttga gcccgcccgg gactacgcca acgataaaac caggggcctg attactgatg
     2941 ctggcttacg tgattccatc aaggcttttc tcgattctca ctcattgagc aaaccgatga
     3001 tcgaacaaag cgatagcagg aaagagacaa tcgtctcagc acacggcgaa ttattggtgt
     3061 atgcgattga gttggaacac caataggccc tgttgggcgc cattttcccc aattgtcacc
     3121 tggaccaact tctcactttc cgcattgacc tgcagtcgac gcaggcataa catgctcaca
     3181 ttcaaacgca tttcgatatc ctaggtgtcg aatctccagt cttccgtctc taacccgttc
     3241 tctgaggaat tttcttgctt ttgaacttac cggatggtgg tttttcgacg aacttctcgc
     3301 gtagatagat agttgagaaa ttgagctttc actaaactgt tttaagctgt ctatcaatct
     3361 cctacgacat tggtcgaatt caatgtccaa gcacacaagc ccccaaaccg gattgtgtaa
     3421 aacatcacca aactttaagg aggattaaca cgatgccaac caaatggaca attagtgcta
     3481 atgatgttag tggtggtagc agcggtagag atctcgttgg ttgccacatc acgatagttg
     3541 gcggaatcgt ttatcagttt acggctccga acaacgacga actggccacg agtcccggtc
     3601 ccacgcttcc aaatctccca tatacttttc ccacagtgca gaattacaag aaccataact
     3661 ggactattaa gctaagaact ctcacttacg gtccgagcaa taaccaggct cagggaaact
     3721 ggtccaacga cgccgcagga attaccgctg gggaaggggg agactggaca gcccaggccg
     3781 gagcgactat tgacg
//
LOCUS       DASSFP010000386         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1444783, whole genome shotgun sequence.
ACCESSION   DASSFP010000386 DASSFP010000000
VERSION     DASSFP010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444783"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /gene="asd"
                     /locus_tag="VFH91_08665"
     CDS             complement(<1..834)
                     /gene="asd"
                     /locus_tag="VFH91_08665"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET6863108.1"
                     /translation="MTKYRVGILGATGMVGQRFIQLLENHPQFTITAVAASDRSQNKR
                     YGDACAWRLQGEMPANIKSLLVQPPKPELDCDLVFSSLPGDIARETENSFAEAGYPVI
                     SNSSAFRMDEDVPLLIPEVNPQHLELIEVQRQRYSSGGFIVTNPNCSTIMVALALAPL
                     HAAFGVEAAIVTTLQALSGAGYPGVASLDILDNVLPYIGGEEEKIETETKKILGKLNG
                     EKVSQAAMAVSAQCHRVNVSDGHMAAIRIKLNKRVEMDQLREALTSFRSIPQELQLHS
                     AP"
     gene            complement(932..2074)
                     /locus_tag="VFH91_08670"
     CDS             complement(932..2074)
                     /locus_tag="VFH91_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6863109.1"
                     /translation="MSEFVPPKRLQGIEKSVIRQVFDRAKPGSLNLGLGEPDLPTPNV
                     IRDAAVKVIVEEQNGYTSHAGLPLLRQKIASDYPYLEKNSDRVIVTAGSQEALYLALL
                     SLVDEGDEVLLPNPGFVAYPTIVKMAGGTSSFYKMPAAADFAFDADAFKRALTPRTKV
                     VVCISPSNPTGRTLSREDLRVIAETLKGHGAYLISDEIYRDLYYGDERPESASTFYER
                     TIVISGVSKSLSMTGWRLGWICGDVDVIRTALILHGYVTTCASAVSQKAALAAWSNEA
                     ELAKANFRTTFGIRRKHLLSLFEDELELRAVAPDGAFYTMLDVSQYGPSVQVANELLE
                     RKVITVPGVAFGSESEGFLRVSFCADLEALKEGVHRIKEGLNSIRA"
     gene            complement(2071..2904)
                     /locus_tag="VFH91_08675"
     CDS             complement(2071..2904)
                     /locus_tag="VFH91_08675"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET6863110.1"
                     /translation="MQLREQIESIAARPGRDFGSEDRKLFEEFKSALNRGEVRAAERA
                     ADGKWTVNTWVKQGILLGFRMGVLADMSAARSLTFYDKDTYPTRPTTIEENVRIVPGG
                     SSIRDGAYLAPGVVCMPPMFVNAGAYVDDGTMIDSHALVGSCAQIGKRVHLSAAAQIG
                     GVLEPVGAMPVIIEDDVLVGGGCGVYEGTVVRERAVLAAGTILTGATPVYDLVEGKIY
                     QRKADAPLEIPSGAVVVPGSRAVENEYGKKWGLSLYAAVIVKYRDAKTEASVQLEDYL
                     R"
     gene            complement(2891..3814)
                     /gene="dapA"
                     /locus_tag="VFH91_08680"
     CDS             complement(2891..3814)
                     /gene="dapA"
                     /locus_tag="VFH91_08680"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET6863111.1"
                     /translation="MTIETAWMRGCATALVTPFTDTGAIDEKCLRELIEHQINNGIRL
                     LVPCGTTGESATMSDEEDQLVIRSTIEAARGRARVIAGTGSNSTATAIQYSQLAESFG
                     ADAILSVAPYYNKPTQEGLYAHFRAIAEAVPNLPVVIYNVPGRTSSNIAAQTTIRLAR
                     DVENIVAVKEASGNLSQIMEIISGKPDGFHVISGDDSMTLAIIALGGEGLISVASNEI
                     PGLMSRLVNLALEGDWDEARSLHYRLLPLMEANFIESSPGPVKAAMSLLGLLSENLRL
                     PLVPVQEKTRERLKEILLELDLFKETSHATA"
BASE COUNT          967 a         1071 c          924 g          954 t
ORIGIN      
        1 tggtgcagag tgtaactgca actcctgcgg aatcgatctg aatgacgtca gtgcctcacg
       61 aagctgatcc atttccacgc gcttgtttaa ctttattctg atcgcggcca tatgaccgtc
      121 agaaacattg acgcggtgac attgagcact gaccgccatt gcagcctgtg aaactttctc
      181 gccgtttagc ttaccgagaa tcttctttgt ctcagtctcg atcttctcct cttcaccgcc
      241 gatgtagggc aacacgttat ccagaatgtc cagtgatgcg actccggggt aaccagcacc
      301 tgaaagcgct tgcagtgtgg tgacaatcgc agcttcgaca ccaaacgcgg catgcaacgg
      361 agccaaggca agcgcaacca ttattgtcga acaatttggg ttagtcacaa taaacccacc
      421 cgatgagtat cgctgtcgtt gaacttcaat cagttcgagg tgctgaggat tcacctcggg
      481 gatcaacaac ggcacatctt cgtccatcct gaaagccgac gagttgctga tcacgggata
      541 gccagcctcg gcaaaactat tttcagtttc gcgagctata tctccgggaa gactggaaaa
      601 caccagatca caatcgagct cgggtttcgg cggttggacc aacagcgatt taatgtttgc
      661 cggcatttcg ccctggagtc gccatgcaca agcatcgccg tatcgtttat tctgcgagcg
      721 atcagaggcc gcgacggccg taatggtaaa ttgaggatga ttctccagca attgaataaa
      781 tcgctgtcca accattcccg ttgccccaag aatgccaact cgatatttag tcatgtttca
      841 tctctattgt gggggcggca ctccgtggcc gctcgaccca ttaaaattca caattacttt
      901 atctaaatgg ggtggccaca gtggaccgcc cctaagcgcg gattgagttg agcccttcct
      961 ttatgcgatg cacaccctct ttaagagcct cgaggtctgc gcaaaaggag acacgcagga
     1021 atccttcact ctcactgcca aaggcgacac ccggcacagt aatcaccttt ctctcgagaa
     1081 gttcgtttgc cacctggacg gatgggccat attgactgac atctaacatc gtgtagaaag
     1141 caccgtctgg agccactgcc cgcagttcca attcatcttc gaataatgag aggagatgtt
     1201 tgcgacgaat gccgaacgtc gttcggaaat tggccttcgc tagctcggct tcattagacc
     1261 atgctgcgag cgcagctttt tgcgaaactg cagaagcaca ggtcgtgacg tagccgtgca
     1321 ggatgagcgc agttctaatc acgtctacgt cgccgcaaat ccaaccaagc ctccaaccag
     1381 tcatgctcag tgatttggaa acaccgctaa taacgatggt tcgctcgtaa aaggtagaag
     1441 cagattccgg ccgctcatca ccgtaataga gatctcgata gatctcatca ctgatcaaat
     1501 aagccccatg tcctttcaaa gtctcagcaa ttactcgcaa atcttcgcgt gagagtgttc
     1561 gtcccgttgg gtttgatgga ctgatgcaca cgacgacttt ggttcggggc gtcaatgcac
     1621 gtttaaatgc atctgcgtcg aaagcgaagt ctgctgccgc cggcattttg tagaacgacg
     1681 acgtaccacc ggccatcttt acaatcgtcg ggtaggcgac aaagcctggg tttggcagta
     1741 atacttcatc tccttcatca accagtgaga gtagcgccag gtacaacgct tcctgagaac
     1801 cggcagtaac aattacccgg tcagagttct tttcaagata ggggtaatcg ctagcaattt
     1861 tctgacgcag caagggcaaa ccagcatgcg aggtatagcc attctgttct tcaacgatca
     1921 ccttaacagc cgcatcacga atgacgtttg gcgttggcag gtccggctcg ccaagaccaa
     1981 ggttcaaact gccaggttta gcccgatcga agacctgtcg aattacactc ttttcgattc
     2041 cctggagtct ttttggcgga acgaactcgc tcaacggaga tagtcctcca gttgcactga
     2101 agcttcggtt ttcgcatcac gatatttcac gattaccgcg gcgtaaagcg ataaacccca
     2161 ttttttaccg tattcatttt cgaccgcacg tgatcctgga acaacgactg caccggaggg
     2221 aatttcaagc ggcgcgtcag ccttcctttg atagatcttg ccctcgacga gatcgtaaac
     2281 cggagttgct ccagttagga tcgttcctgc ggcaagaaca gctctctctc tcacgaccgt
     2341 gccttcgtac actccgcaac cgcctccgac taaaacgtca tcctcaataa ttaccggcat
     2401 cgccccaact ggttctaaca ctcctcctat ttgggcggcg gctgagagat ggactctctt
     2461 tccaatctga gcgcacgaac caaccagcgc atgactgtcg atcattgtgc cgtcgtcgac
     2521 gtacgcaccg gcattaacga acatcggcgg catacagaca acgcctggcg ccagataagc
     2581 tccatctcga atgcttgagc caccgggcac aatcctcaca ttttcttcaa tagtcgttgg
     2641 ccgggtcgga taagtgtctt tgtcgtagaa ggtaagactg cgcgctgcag acatatccgc
     2701 aagcaccccc atgcgaaagc caagtagaat gccctgctta acccaggtat tgacggtcca
     2761 tttcccatca gcggctcgtt cggcagctcg aacttctcca cggttgagtg cggacttaaa
     2821 ctcctcgaag agtttgcgat cttcagagcc gaagtctcgt ccgggccggg ccgcgatact
     2881 ttcgatttgt tcacgcagtt gcatgtgaag tttccttgaa caagtcaagt tcaagcaaga
     2941 tctcttttaa tcgctcgcga gtcttttcct gtaccggtac aagtggaagc cgtaaatttt
     3001 cgcttaacag accaaggaga ctcatcgctg cttttactgg accaggactc gattcaataa
     3061 agttggcttc catcaaaggc aacaaacggt aatgcaaact acgcgcttca tcccaatcgc
     3121 cctcgagcgc tagattgaca agtcgagaca tcaagccggg tatttcgttg gaagcgactg
     3181 agatcaaacc ctctcctccc aaagcgatga tcgcgagcgt catcgaatcg tcgccggaaa
     3241 tgacgtggaa accatccggt ttgccactaa tgatctccat aatttgagac agattgcccg
     3301 atgcttcctt aacagcaacg atgttttcga catcgcgcgc aagtctgatg gttgtttgag
     3361 ccgcaatatt agacgaggtc cgacccggaa cgttgtagat aaccacagga agatttggaa
     3421 cagcttccgc tatggctctg aaatgcgcgt agagtccttc ctgagttggc ttattgtaat
     3481 aaggagcgac tgagagtatt gcatcagcac caaaggactc tgccaactgg gaatactgaa
     3541 ttgctgtggc cgttgaattg ctcccggttc cagcgataac tcttgctcgc ccacgcgcgg
     3601 cctcaatcgt tgagcggata accagctgat cctcttcatc cgacatcgtg gcgctctcac
     3661 cagtagttcc gcatggcacg agaagacgaa ttccattgtt gatttgatgt tcaatcaatt
     3721 cgcgcaaaca cttctcgtca atagctccgg tgtctgtgaa aggagttacg agagctgtag
     3781 cgcaaccacg catccaagcc gtttcaattg tcatgatcta acctcctaaa caattatgtt
     3841 attgacgagc gactcaaaat ctcgtcgatc acatgatcga actcaaacat cccggtctta
     3901 ccgacgatcc agtgag
//
LOCUS       DASSFP010000387         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_419739, whole genome shotgun sequence.
ACCESSION   DASSFP010000387 DASSFP010000000
VERSION     DASSFP010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419739"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFH91_08685"
     CDS             complement(<1..196)
                     /locus_tag="VFH91_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863112.1"
                     /translation="MNRFFAIVTTTLLIAALSTLSFAQTSPPPVPTSKIALFNLEDLR
                     PGMKGTARTVFSGTETEEFGV"
     gene            502..1098
                     /locus_tag="VFH91_08690"
     CDS             502..1098
                     /locus_tag="VFH91_08690"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET6863113.1"
                     /translation="MNRVEGFILAGGSSSRMGRDKAQLRIAEKTFSERIAAELLSFTS
                     TVTMIGGSTIPQNLNSAKDVYSQIGALGGLHGALAACNADWALVVACDLPFVTKELFD
                     KLAASRENFEAVVPVQEDERPQPLCALYKINPCLRVASELIEGGKRRPLDLLDRVKTR
                     WIPFAELKPLNRAKEFFVNINTPEDYDEATRKHSATTT"
     gene            1147..2130
                     /locus_tag="VFH91_08695"
     CDS             1147..2130
                     /locus_tag="VFH91_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863114.1"
                     /translation="MSPRRRIGRKFIRSFTPIVLVIVLALLGFLAFAAYCVTHPSKRP
                     YLVTPQSFSNISGTVLKVTDETWSSLDGKRQRGWLLKGAPGSPAVVLLHTYGADRSWL
                     FNLGVKIYETTNFTILWLDLRGHGENPLVKWTSLGAREGEDVVSALNFLRTLKADNQA
                     KLVNDRFGVYGVELGGYAAIKGGGIDNQIQVLVLDSIPSSPDQLLNNKLTSCIGVDSN
                     FLLSLTRAATKFYLLGAYENTSTCDLVRTLRDKQVLVLAGSDSGDLKQTTMDLRSCFQ
                     NSVTVEMRTDLPLSGLNLPSATGEQGEAYDRIVIEFFDRHLTGTSPGRPAK"
     gene            complement(2107..>2517)
                     /locus_tag="VFH91_08700"
     CDS             complement(2107..>2517)
                     /locus_tag="VFH91_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863115.1"
                     /translation="YTLGWTRRRDVAVVSIAVATCLMAAMVLECAAPGFGRRESVRDL
                     LRAADERGYAGLPVLAQRGDDRTTEFYASGRVVYRADGEILPVDEVSIDQARKLGRML
                     VFVSKEHVDYVLRVPGMELLGDNGRVALLCWTPR"
BASE COUNT          648 a          641 c          624 g          604 t
ORIGIN      
        1 cgacgccaaa ctcctctgtc tcagttccac taaagacagt tcgcgccgtg cctttcatgc
       61 cgggacgaag atcttcgaga ttgaacaaag caatcttcga agttggcacg ggcggagggg
      121 acgtttgagc aaacgataga gtactgagtg cagcaattaa gagagtggtt gtaacaattg
      181 caaaaaatct attcattaac ggcctcttat atggattcct tcaaagcaaa tcagctatca
      241 aacctagctg tcatatagat gccaaaaaag caccgcgtga caccaagcga ctataaactt
      301 cgcaaaagaa tgtggtcaag gttttcagtc ccaaggattt gacacccgtt ctcagcatgc
      361 tgttcccggc ggtaatgaat ttcgcgcaag gatcatatgg gctatttgac ccttctaatc
      421 cgttgatcgt tgctaagaac gcatgttaac gtcgctttac ggcactgggc cgccactttc
      481 accagttaca aagtcagaac aatgaatcga gttgaaggtt tcatcctcgc tggcggatcc
      541 tcaagcagaa tgggacgcga caaagcgcag ttacgaattg ccgagaagac cttctccgag
      601 cggatcgcgg ccgagttact cagctttacg agtacagtca ccatgattgg tggctcaacc
      661 attccacaga atctgaactc ggcaaaggat gtgtatagtc aaattggtgc gctgggaggg
      721 ctgcatggag cgctggcagc ctgcaacgct gattgggccc tggtggtcgc ctgcgaccta
      781 ccgtttgtca cgaaggagct attcgacaag ctggctgcct ctcgtgaaaa ctttgaggct
      841 gtagttccag ttcaggagga tgaacgtcct cagccattat gcgctcttta taaaattaat
      901 ccgtgtctga gagtggcgtc tgagttgatc gaagggggta agcgtcggcc gttggaccta
      961 ctggatcgag taaaaacgag atggattcct tttgcagaat taaagcctct caaccgcgcc
     1021 aaagaatttt ttgttaacat caatacgccg gaagactatg atgaggcgac tcggaagcat
     1081 tccgcaacaa caacctaacc tgagtcgggt ctgagagatg catagccgga acctttacaa
     1141 atactgatgt caccccgacg aagaattggc agaaaattta tcaggtcctt cacgccgata
     1201 gtgcttgtaa ttgtgctcgc gctactcgga tttcttgctt tcgcagcata ttgcgttact
     1261 catccgtcga aacgacccta cctggtaacg ccgcaatcgt tctcgaatat aagcggaact
     1321 gtcctgaagg ttactgacga aacatggtcc tccctggatg ggaagcgaca gcgtggctgg
     1381 ctattaaaag gagctcctgg ttcgccggcg gtggtcctgc tgcacaccta cggagccgat
     1441 cgttcctggc tttttaacct cggggtcaag atctacgaga cgaccaactt tacaattctt
     1501 tggcttgatc tcaggggaca tggcgagaat ccgctggtta agtggacctc acttggagca
     1561 cgcgaaggcg aagacgttgt atcagctctc aacttcctca gaacactgaa ggctgacaat
     1621 caagcaaaac ttgtaaatga tcgttttggt gtctatggag tcgaactggg tgggtatgcg
     1681 gcaataaaag gtggaggcat tgataatcag attcaagtgc tcgtgctcga ctcgatcccg
     1741 agttcacccg atcaactgct caataacaag ctcactagtt gtattggagt cgatagcaat
     1801 tttctgctct cgctcacccg tgcggctact aaattctact tgctgggagc ttatgagaac
     1861 acgagtactt gtgacttagt ccgaacgtta cgcgacaaac aagttctggt cctggctggt
     1921 tcagactctg gcgatctgaa acagacaact atggaccttc ggagctgttt ccaaaattcg
     1981 gttacggttg agatgaggac tgatttaccg ctgagcggcc ttaatcttcc ctccgctacc
     2041 ggagaacaag gcgaggccta cgatcgaata gtgattgagt tttttgatcg acacttgaca
     2101 ggtacttcac cggggcgtcc agcaaagtaa cgcgacacga ccgttgtcac ccagcaactc
     2161 cattccggga actctcagga cgtaatcgac atgttccttc gagacgaata ccaacatcct
     2221 tcccaatttt cgtgcctgat cgattgaaac ctcatcaacc ggcaagattt cgccatccgc
     2281 gcggtaaaca actctgccac tcgcatagaa ttctgttgtg cgatcgtcgc cccgttgggc
     2341 caacacgggc agacctgcgt agccccgttc atcagcagct cgcaacagat ctctgacgga
     2401 ttcgcgtcta ccaaaaccag gcgccgcaca ctcgagaacc atcgctgcca ttagacaggt
     2461 agcgacggca attgaaacaa ccgccacatc acgacgtctg gtccatccga gagtgta
//
LOCUS       DASSFP010000388         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1145714, whole genome shotgun sequence.
ACCESSION   DASSFP010000388 DASSFP010000000
VERSION     DASSFP010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145714"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="VFH91_08705"
     CDS             <1..1816
                     /locus_tag="VFH91_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863116.1"
                     /translation="LEVAPPHLAQFDKDKNYGDFSLARNDTLENVIIDDGKDSIIDIP
                     LAQEQKPADQQSKATPDRWRGLFPEYERYGDRGARGRDIPFKNGRWWDPYNQSVLKGD
                     FPIHGNKFFMNLSAVSSTNVEQRRAPTPSDVSSADPGSGEFFGRPEQFAATETVQLSF
                     ELFHGETTFKPRDWAIKISPTFSVPNYLNARENGVVNIDVRRGTNRTDWHVSLEEAFG
                     EVKLADVNANYDFISVRAGIQPFVSDFRGFIFSDNNLGARVFGSLDNNRVQYNAAFFP
                     MLEKDTNSALNTFDSRHQNVYIANIFRQDFIRKGYTIQGSFHYNDDRRSVEFDRNGFL
                     VRPALIGDVRPHAIKVAYVGVSGDGHLGRLNLSHSYYYAFGHDERNEIASRRVQVRSN
                     MAAVEASVDRDYLRFKGSFLWAQGDSNPTDDRGTGFDAIFDDPNFAGGQFSFWVRNGI
                     RLSQTGVGLVQPNSLLPTLRSSKIQGQANFVNPGIFVYNGGVDAELTQRIKAVFNVNY
                     LRFHRTEPLEYILLQNRIRHDIGFDYSLGVAYRPFLINNVTLTFGAATLQTGRGFRDI
                     FTDASRNCPPVVDEFCEPGVISPNKALYTLFAQMKFVF"
     gene            1850..>4187
                     /locus_tag="VFH91_08710"
     CDS             1850..>4187
                     /locus_tag="VFH91_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863117.1"
                     /translation="MKRSRQLVLLLVLAIGVSALGQTAIGPRLVQQVATAQSNPPAKA
                     EGCVSCHGLIEPMHKYGTTETLDKLKDGKDAVGLTCTGCHGGNPVPAKTSSAAGEVER
                     VKKLAHVRPRYPREWQRAGKYTGANPERTNTLLARESWEFVRFVNPGDLRVVGKTCAG
                     SACHDVESKNVSRSMMTHGAMLWGAALYNNGSFHLKDARFGESYTTAGEPQTLTQNAT
                     EEQQRTKGMLSWLDPIPRWEVSQPGNVLRVFERGGKRRLEVGLPDKEEDPGKPDKGLS
                     ARGFGTAQRTDPVYLGLQKTRLLDPTLNFLGTNDHPGDYRSSGCSACHVIYANDRDPV
                     ASEFYSQAGNLGRSQTSDTNIPREESGHPIKHQFTSQIPTSQCMVCHMHPGTNMVASY
                     LGLTWWDNESDGSKLYPAKQHDPSQDEEREKLDRNPEGASLRGLWANFDFLKDTGTAD
                     FNSLLRNTQFADSHGHGWLFRKVYKRDRKGNLLDADGKIVSPDDPLKFSKAVHLNDIH
                     LEKGMHCVDCHFRQDVHGDGNLYNEPRAAIEISCEDCHGSIRQRANLFTSGSAAAPAT
                     GAAAERRKTQNQPMNGQDLTRIRVRDESGTRIPLFQRVTRDRKRKDEKGNDVDLKAGD
                     IMQNSMVVPGRWWRVPQTLDTVTPGSRDYNEKSQYAKTIQKDNATWGTVPAESNLAHR
                     DSDMTCVSCHSSWMTSCFGCHLSMQANRKMPNRHNEGGNSRNFTQYNFQVLRDDVFML
                     GRDGTVTGNRVAPVRSSSAVLVSSQNQNREWIYSQQQTT"
BASE COUNT         1085 a         1072 c         1095 g          935 t
ORIGIN      
        1 actcgaagtt gcgccaccac atctcgctca gttcgataaa gacaaaaact atggcgattt
       61 ctcactcgcg cgcaacgaca ctcttgagaa tgtaattatt gatgatggca aagactcaat
      121 catcgacatc cctctcgcac aagaacagaa accggcagat caacaatcaa aagcaactcc
      181 agatcgttgg cgcgggttgt ttcctgagta tgagcgctat ggcgatcgcg gagctcgagg
      241 tcgagacatt ccctttaaga acggtcgctg gtgggatcct tacaatcaga gtgttttgaa
      301 aggcgatttc ccgattcatg gcaacaaatt cttcatgaat ctctccgctg taagctcgac
      361 taacgtcgag cagagaaggg cacctactcc ttcagacgtc agcagcgcag atccgggaag
      421 tggcgaattc tttggccggc ctgaacagtt tgccgcaacc gaaaccgttc agcttagctt
      481 cgaactattt catggcgaaa ccacatttaa gccacgcgac tgggccatca agatatcgcc
      541 aacttttagt gtccccaact acctcaatgc acgcgagaat ggggtcgtca atattgacgt
      601 gcgacgggga acaaatcgaa ctgactggca cgtttccctg gaagaagcat ttggggaagt
      661 aaaacttgcc gacgttaacg caaattacga tttcatctct gtgagagccg gaattcagcc
      721 gttcgtctct gactttcgtg gtttcatctt ttccgacaac aatctcggcg cgcgagtttt
      781 tgggtctctg gataacaatc gagttcaata caatgccgca ttcttcccga tgttggaaaa
      841 agacacgaac agtgctttga atactttcga ctctcgtcat cagaatgtct acatcgcaaa
      901 tatctttcgt caggatttca ttcgtaaggg atacacaatc cagggcagct ttcactataa
      961 cgacgaccgg cggagcgtcg agttcgatcg aaatggattt cttgttcgtc ccgcgctgat
     1021 cggcgacgtt cggccacacg cgatcaaggt cgcctacgtt ggcgttagtg gcgacggaca
     1081 cttaggccgg ctcaatctct cgcactccta ttactatgca tttggtcacg acgagcgcaa
     1141 tgagattgcc agccgcagag tccaggtgag atcgaacatg gccgcagttg aagcgtcagt
     1201 ggaccgtgat tacttgcggt tcaagggttc atttctttgg gcacagggag atagcaatcc
     1261 aactgatgat cgcggtacgg gttttgacgc aatcttcgac gacccaaact ttgcaggcgg
     1321 gcagttttca ttctgggtcc gcaatggaat tcgcttgagc caaaccgggg tgggcctggt
     1381 ccaaccgaac agtttgttac cgacattgag aagcagcaag attcagggtc aggcgaactt
     1441 tgtcaatccc gggatctttg tttacaacgg cggtgtcgac gctgaactaa cccaacgcat
     1501 caaagcagtc ttcaacgtca actatttgag atttcaccgt acagagccac tcgagtacat
     1561 cctgttacaa aatcggataa gacacgacat cggcttcgac tacagtctcg gagtagctta
     1621 ccggcctttt ttgatcaata acgtgacgct tacctttggc gctgcgactc tgcaaacggg
     1681 acgagggttt cgagatatat tcaccgacgc atcgcgcaac tgcccgccgg tagtagacga
     1741 attttgcgag ccgggtgtta ttagtcccaa caaagccctc tacacgctgt tcgctcaaat
     1801 gaagtttgtt ttttaaatcg ccgctgggcc cggcttttct gttctgaaca tgaagagatc
     1861 cagacaactt gtgcttttgc ttgtgctggc aataggtgtt agcgctttgg gccaaaccgc
     1921 cattgggccc aggttggtcc agcaggtagc aactgcacaa tcaaatccgc ctgcgaaagc
     1981 ggagggttgc gtttcgtgtc atggcttaat cgagccgatg cacaagtatg gaacaaccga
     2041 gactctcgac aaactgaagg atggaaaaga cgcagttgga ctgacttgca ccggttgtca
     2101 cggtgggaat ccggtacctg ctaagacaag ttccgcagct ggagaggttg aacgagttaa
     2161 gaagcttgct cacgtacggc ctcgttatcc gcgcgagtgg caacgagcgg gcaaatacac
     2221 tggagcaaat cccgaacgaa ctaacacact cctggcacgc gagagttggg agtttgtgcg
     2281 gttcgtaaac ccgggcgatt tgagagtagt cggaaaaacc tgcgccggta gcgcgtgtca
     2341 cgacgttgag tcgaagaatg tttcgcgcag catgatgact cacggagcaa tgctttgggg
     2401 cgcagcactg tataacaacg gcagctttca ccttaaagac gctcgctttg gagagagtta
     2461 caccaccgca ggtgagccac aaacgctgac acaaaacgca accgaagagc agcaacgaac
     2521 aaagggaatg ttgagttggc tggatccgat tccgcgctgg gaggtgtcgc aaccaggcaa
     2581 cgtcctgcgc gtttttgagc gaggaggaaa acgcagactc gaagttggac tgccggacaa
     2641 agaagaagat cctggtaagc ctgacaaagg cttgagtgca cgcggcttcg gaacagcaca
     2701 gcggacagat ccggtttacc tcggtctgca gaagacacgg ctgctggacc ccaccctcaa
     2761 tttcctcggc acaaatgatc atccgggcga ctatcgctca tcgggatgct cagcttgcca
     2821 cgtgatctat gcaaatgatc gcgatccagt agcgtctgaa ttttacagtc aagccggtaa
     2881 cctcggaaga tcgcagacca gcgatacaaa cattccgcgc gaagagtcag gccatccgat
     2941 caaacatcag ttcaccagcc agataccgac gtcgcaatgc atggtgtgcc acatgcaccc
     3001 cggcacaaac atggtcgcga gttacctggg ccttacctgg tgggacaacg aaagcgatgg
     3061 ttcaaagctc tatcccgcaa agcaacatga cccgagtcag gatgaggagc gggagaagct
     3121 cgatcgaaat cccgaaggcg ccagcctgcg cgggctctgg gccaactttg attttcttaa
     3181 agatactggc accgctgatt tcaactcgct gttacggaat acacagttcg ccgactctca
     3241 cggtcacgga tggctcttcc gaaaagtcta caagcgggat cgaaagggga acttattgga
     3301 tgctgatggc aagatcgtct cgccagatga tccattaaaa tttagtaagg ccgttcatct
     3361 caacgacatt catctggaaa aaggtatgca ctgtgttgat tgtcatttcc gtcaggacgt
     3421 tcacggcgat ggcaatctct ataacgagcc acgcgccgcc atcgaaatca gttgcgagga
     3481 ttgtcatggc tccatccggc aacgtgcgaa tctttttact tccggatcgg ccgccgcgcc
     3541 ggcgaccgga gccgcagctg aacgccgtaa gactcaaaat caaccaatga atggccagga
     3601 cctcaccaga atcagagtta gagacgaaag cggcacacga atccctctct tccaaagagt
     3661 gactcgcgac cggaaacgaa aggatgagaa gggaaatgat gtcgatttaa aggcaggcga
     3721 catcatgcag aactcgatgg tcgtacctgg acgctggtgg cgtgttccgc agacgctcga
     3781 tacagtcact ccgggatcgc gcgattacaa cgagaagtcg cagtacgcaa aaacaattca
     3841 gaaagacaat gcgacctggg gtactgtgcc ggcggaaagt aacttggccc atagagattc
     3901 ggatatgaca tgtgtttcgt gtcattcatc atggatgaca agttgttttg gttgtcactt
     3961 gtcaatgcag gcaaatcgca aaatgcccaa ccgtcacaac gaaggcggca atagtcgaaa
     4021 ctttacccag tacaactttc aggtcttgcg cgacgatgtc ttcatgctgg gacgcgatgg
     4081 tacggtcaca gggaatcggg ttgctccggt acgttcgtca tcagctgtgc tggtgagctc
     4141 tcaaaatcag aatcgtgagt ggatctactc gcaacaacag accactt
//
LOCUS       DASSFP010000389         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1604007, whole genome shotgun sequence.
ACCESSION   DASSFP010000389 DASSFP010000000
VERSION     DASSFP010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604007"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1097)
                     /locus_tag="VFH91_08715"
     CDS             complement(81..1097)
                     /locus_tag="VFH91_08715"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET6863118.1"
                     /translation="MRYVENPPNPWLTQSVEWIGEPPQIKLEVFEETDTRKIITHNDS
                     PDVGFDYTVNCYRGCIHGCTYCFSRPTHEYLGYGAGTDFDRKIVVKVNAPKLLRQELM
                     KSSWKGDEIVFSFTSDPYVPLESHYKLTRQCLEICAEFHQPIGIITKSALIRRDIDVL
                     QELSRNASLAVFFTIPFTDVETARAVEPYAPLPEARFHAMQKLADAGITVGIGIAPVI
                     PGLSSDIPELLKRAKECGAEMAFINMLRLPGSVAPYFEERLRDELPTKADRVMSRIRE
                     ARDGKVNSSVFGERMRGKGEYWKAVERLFKIHSQRLGFNKKRTHQTASTSTFRRPTAQ
                     ASLF"
     gene            complement(1115..1330)
                     /locus_tag="VFH91_08720"
     CDS             complement(1115..1330)
                     /locus_tag="VFH91_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863119.1"
                     /translation="MARQHTENLFRKFKGELITLKTISGGLYSGRITEISNDHICLNE
                     TLGEEGVQVYVFYDSIESLTVGGEKSK"
     gene            complement(1342..1731)
                     /locus_tag="VFH91_08725"
     CDS             complement(1342..1731)
                     /locus_tag="VFH91_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6863120.1"
                     /translation="MLTGLDLIFLEVNSLEESLAFYKEVLGFEIDSINSAAEPPTATI
                     HAGRLKVTLAQNLEMMLRRGRGVHFFVGVDDIDTTYRQIQIKNVEVQPPVDEGWGGRF
                     ITLEDPDKYRWFLVGWSTTDPDPDASE"
     gene            complement(1731..2291)
                     /locus_tag="VFH91_08730"
     CDS             complement(1731..2291)
                     /locus_tag="VFH91_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET6863121.1"
                     /translation="MSRPDTSKDYYDVLGVAEEASKTEIDRQYKKQAARHHPDLGGSE
                     EQMKSLNEAYGVLKDQDARKAYDNSRRRYTRTARYVPVSAPPASDFGVFGHCLSALLC
                     LVAGMFLLLLVRFQWIWFLWPLAILAMFVIGFGVLLARSAMVAVNASLPLTHRLRRHT
                     NLQELAFWLVVVSGAYGLYLLIASDY"
     gene            2420..>2619
                     /locus_tag="VFH91_08735"
     CDS             2420..>2619
                     /locus_tag="VFH91_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863122.1"
                     /translation="MAHTFAPGDDLIFQLESAFGLLRVLAIEGEGPQTVWHLLAYDDF
                     FPDVESAERALASNTSMPIRNA"
BASE COUNT          651 a          697 c          600 g          671 t
ORIGIN      
        1 ccgcagatta cacccacctc gttttgaaaa tcggcgtaat cagcgcaatc tgtggatgat
       61 tattggactc ccctgtaacc tcagaacaaa ctagcttgag ccgtgggtct gcggaatgtg
      121 ctggtggacg cggtctgatg agttcgtttc ttgttgaatc ccaaacgctg agaatggatc
      181 ttgaacagcc gttcaaccgc tttccagtac tcgcctttgc cgcgcatcct ttccccaaat
      241 acactcgaat ttactttacc gtcacgagct tcacgtattc ggctcatcac gcgatcagcc
      301 tttgtcggaa gctcatcgcg taaccgttct tcgaaataag gagcaacact gcctggcaag
      361 cgcagcatgt ttatgaaggc catttcggct ccgcactctt tggcgcgttt aagtaattcg
      421 ggaatatcac ttgaaagacc gggaattacg ggagcgatcc cgataccgac cgtgatgcct
      481 gcgtctgcaa gcttttgcat cgcgtggaag cgagcttcgg gcaatggagc ataaggttcg
      541 actgcccggg cagtttcaac atctgtaaat ggaattgtaa agaacacagc aaggctggcg
      601 ttgcgcgata attcctgcag aacatcgatg tcacggcgaa tcaaagctga cttagtgatg
      661 atcccgattg gctggtggaa ctccgcgcag atctccagac actgtcgagt taacttgtag
      721 tgcgactcca gtggaacata cggatcagag gtaaaggaaa agactatctc atcacctttc
      781 caggatgatt tcatcaattc ctgtcgcagt aatttgggcg cattcacttt aacaacgatc
      841 tttctatcga aatcggttcc cgctccgtag cccagatact cgtgggtcgg tctactgaaa
      901 cagtacgtgc aaccgtgaat gcaaccgcga taacagttga ccgtgtagtc gaatccgacg
      961 tcgggactat cgttgtgggt gatgatcttc cgagtgtcag tctcttcaaa tacctcaagc
     1021 tttatttgag gaggttctcc tatccactcg acagactgtg tcagccatgg attcggcggg
     1081 ttttcgacgt aacgcatacc cttgttccct gaaattattt tgacttctcg ccgcctaccg
     1141 tcagcgattc aattgaatcg tagaagacat atacctgaac gccttcttca ccgagcgttt
     1201 cgttcagaca aatgtggtcg ttgctgattt cggtaatacg cccgctgtag agcccacccg
     1261 aaatcgtctt caaggtaatt aactctccct taaactttct gaacagattc tctgtatgtt
     1321 gtctcgccat catgactcct ttcactcaga agcatctggg tcaggatcag tcgtagacca
     1381 gccaaccaaa aaccagcgat atttatccgg atcttcgagg gtgataaacc gtccacccca
     1441 accttcatcc accgggggtt gcacctcgac gttcttaatc tgaatttgtc gatacgtggt
     1501 gtcgatatca tcaactccta cgaagaagtg tacgccccgg cctcgacgta gcatcatttc
     1561 cagattctga gcgagtgtca ctttcagtcg tcccgcatga atggtggcag ttggtggttc
     1621 ggccgcgcta ttgattgagt caatctcaaa gccaagcacc tccttataaa acgccagcga
     1681 ctcttctaaa ctgttcactt cgagaaatat taggtcgagg cctgttagca tcaataatca
     1741 ctcgctatca acagatatag gccataggct ccggagacga cgaccagcca gaatgcaagc
     1801 tcttgtagat tcgtgtgtcg tcgtaagcga tgggtcaatg gaagagatgc gttcaccgcc
     1861 accattgcac tgcgcgctaa cagaactcca aatccgatca cgaacatcgc aagaatcgcc
     1921 agcggccata gaaaccagat ccattgaaaa cggaccaaca gcaacaaaaa catcccggca
     1981 acaagacata gcagcgcact caaacagtgc ccaaacacac caaagtcgga agcgggcggc
     2041 gctgaaaccg gaacgtatct ggcagttctc gtgtaacgtc tgcgactgtt gtcataagcc
     2101 tttctcgcgt cctgatcctt caaaaccccg taggcttcgt tcaaagactt catctgttcc
     2161 tcgctgccac cgaggtctgg atggtgtcgc gcagcctgct tcttatattg ccgatcgatc
     2221 tcagttttcg acgcctcttc ggcgacacca agaacgtcgt aatagtcttt acttgtatcg
     2281 ggacggctca tcggtttgct gattctagga gccggatttt cgtttggcaa tccatatcgg
     2341 cacctaaaac aactaacaaa tttcaaatga taattgatcg ttcatcctgt gctaacctta
     2401 ttccgagaca cataacctta tggcacatac tttcgcaccg ggcgatgacc tgatttttca
     2461 gctcgaaagc gctttcgggt tacttcgtgt actcgcaatt gaaggagaag gtccacaaac
     2521 ggtgtggcac ttgcttgcct acgatgattt ctttcccgac gttgaatccg cggaaagagc
     2581 gcttgcgagc aacacgtcta tgcccattcg aaatgctca
//
LOCUS       DASSFP010000390         6027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_264447, whole genome shotgun sequence.
ACCESSION   DASSFP010000390 DASSFP010000000
VERSION     DASSFP010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264447"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFH91_08740"
     CDS             complement(<1..194)
                     /locus_tag="VFH91_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863123.1"
                     /translation="MLLNVTYNSPTERNSRRNQEEKAHPIHKCYCFIVHGNLWSAAAR
                     RRFVIIESRLTTSKEETALF"
     gene            complement(391..726)
                     /locus_tag="VFH91_08745"
     CDS             complement(391..726)
                     /locus_tag="VFH91_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET6863124.1"
                     /translation="MIVEVRSYRIKPGKRAEFIKFFQTKAVPALRSHGMKIQGPMLDL
                     ENPNKFVWLRSFPSLEERERMKNEFYEGPLWKNEMEGIAMPMLESYDVILCETAPGYV
                     FDGPVSTVE"
     gene            783..1154
                     /locus_tag="VFH91_08750"
     CDS             783..1154
                     /locus_tag="VFH91_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6863125.1"
                     /translation="MDDNKKVPSGNHQALVRSNPVTGCPLTAALAAIGGKWKMIIVYW
                     LAESPKHFAALRRAMPGISQKVLTQQLRELVNDGLIERQPHGVVPAKVEYSLSDYGRS
                     VLPLIENVRVWGRNHMERPGD"
     gene            1177..1725
                     /locus_tag="VFH91_08755"
     CDS             1177..1725
                     /locus_tag="VFH91_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863126.1"
                     /translation="MRSVIVILTLFLTLPIAAQTKPPAVYVDRGACPFECCTYRTWKT
                     DKDTVAYAQPNKSSRRVGVLKARTNVAALTGEVRTWPGKFTIIKAHDKYKPGDVLWVY
                     TPLGEGFYKVWFKGQMTEQELDYMSGPFEQTFPRCEDAKDCWGQLESPLRVEWWVKIK
                     SKDGWVGWTNETENFSNQDACG"
     gene            1768..2304
                     /locus_tag="VFH91_08760"
     CDS             1768..2304
                     /locus_tag="VFH91_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863127.1"
                     /translation="MDQTHKTIYITLVLCFTVVFQGCSRSYPQLDWSELTPLCKQQAL
                     NATALLTSEKELAKIEELDAALYKPIAGLTIFLESQTPTFDSRNLKPRYWLRVEDYSS
                     TAIASQRATEYSSVDTYERIARASGGDSFIVSKTTVRLWAIARGKRVYALTTDTNLFG
                     LIEMPVKLRKTIGMLPER"
     gene            2315..2881
                     /locus_tag="VFH91_08765"
     CDS             2315..2881
                     /locus_tag="VFH91_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6863128.1"
                     /translation="MRIAISGTHCSGKSTLAESFLMDHPAYIHEPEAYEALGEFCGEH
                     FSDKPTPGDLFRQLEYHVKRLQHYKEADCVIFERAVVDYVAYIQILEEFAGSALDKNL
                     TSDAIQIAKEHIKLLDAVVFLPLHGFAGDAPVDEDLRLRRRVDSRLEAILLNDELDLF
                     VGAGPLLIEATGTTQQRLADINKLLELN"
     gene            2884..3216
                     /locus_tag="VFH91_08770"
     CDS             2884..3216
                     /locus_tag="VFH91_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863129.1"
                     /translation="MGIVVFLITSAVQFLVAVFGFFILLLALNGYSEQSATPGIVIYT
                     LVSLIIVLGLASIAAFVSTRIVSRKGLGSLAVGSLSTIGFSILGGVFLVGTFFVSVGI
                     AELLRTLK"
     gene            3243..3626
                     /locus_tag="VFH91_08775"
     CDS             3243..3626
                     /locus_tag="VFH91_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6863130.1"
                     /translation="MNQHGEPRITATLSVRNWSKAMAFYKSAFGAVELFSVPGGGVGQ
                     LSVSGAEFWVAEESPEHLNFSPETLGGCSVRMLMIVEDPDSVYQQAINAGATEVVSVT
                     EEHGWRLGRIVDPFGHHWEIAKKLS"
     gene            complement(3838..4284)
                     /locus_tag="VFH91_08780"
     CDS             complement(3838..4284)
                     /locus_tag="VFH91_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863131.1"
                     /translation="MRETSKEVTALLRALARGLEEDRSVDVELAGEQIEVPAYADIRV
                     QYHVRDEKQISIDVIWDRVAEAPELIRRHDDQVQDEAGQVYDVLIYGERVGGKWEGWI
                     DFVPLSAALPSKRTGRETTQPDRAALEYWATGLEPLYLAGAFERAT"
     gene            4479..5183
                     /locus_tag="VFH91_08785"
     CDS             4479..5183
                     /locus_tag="VFH91_08785"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET6863132.1"
                     /translation="MDHSTGSRRALWQVIFSVVYLSFFPPILFWLAGDWRWIEGWIFS
                     ALLCLLSLATVFYLYLKDPALLNERFGSPVQKNQKSWDKVLVVLFIFAFLTWYAIMPL
                     DARQFEWSPHFPIWVNVIGALLFSFGFYLLYRAMRENTYAAPVIKMQKERGQTVIATG
                     PYSIVRHPMYTGGTLVFLGGPLLLGSLYGLLAGFLLSLMLALRTFGEEAMLRTELPGY
                     AEYRTRVRWRLIPYLF"
     gene            5195..5713
                     /locus_tag="VFH91_08790"
     CDS             5195..5713
                     /locus_tag="VFH91_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET6863133.1"
                     /translation="MPLPVAHALVGATVKTSLSESHQALSLKAISLTVLLAICPDFDY
                     ALNFLQIGGGGWHHGFTHSILFAIAIGLLCWLILKDKRPTTLFILCAVVVSHTLLDYL
                     FTESRGVALFWPITDYRYKLNWFNPINYSWRSTSISAAVADLARLCVLELLIFSPVLL
                     LALLIKKRFASK"
     gene            5797..>6027
                     /locus_tag="VFH91_08795"
     CDS             5797..>6027
                     /locus_tag="VFH91_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863134.1"
                     /translation="MRPLKTVLSFCLLLTATTQPVVAQTTSLAEQVKRLQDDPSVKAA
                     NDYIDKHRDPILSEWIAITEINAPSKQEQERAK"
BASE COUNT         1504 a         1503 c         1435 g         1585 t
ORIGIN      
        1 taaaagagtg ctgtctcttc tttagaggtt gttaatcgcg attcgatgat aacaaagcgg
       61 cgccgggccg ccgcactcca aaggttgcca tgaacgatga agcagtaaca tttatgaatt
      121 ggatgggcct tttcctcctg gttccggcgg gaattgcgct cagttgggga attgtaagtg
      181 acgttcaaaa gcataagaaa ctcacttcca acttgatcac ctctatattg ctattcctgg
      241 ccacccttat tggtctgata cagttaaaca tacaatcaca agggtggttg cgctggtcca
      301 tactcctcat tcagctcgtc atcacaatct ggttaactaa aatggtctgg cccccttttg
      361 tccgcaagtt ccgcgggctc gactaaaccc ttattccacg gtactcactg gtccatcgaa
      421 aacatagccg ggagccgtct cacaaagaat cacatcgtag ctctccagca tcggcatcgc
      481 gatcccttcc atctcattct tccataacgg gccttcgtaa aactcgttct tcatgcgctc
      541 tcgctcctcc agcgaaggaa agctgcgtaa ccacacaaac ttattcggat tctcaaggtc
      601 gagcattggt ccctggatct tcataccgtg cgaacgcaat gccggcactg cctttgtttg
      661 aaagaattta ataaactcgg cgcgttttcc aggcttaatc ctgtaacttc ttacctccac
      721 gatcatcttg atacctcccg aaattatcct caacgcaagc acaaacgact ataattccga
      781 acatggacga caacaagaag gtaccttcag gtaaccatca ggccctggtg agatcaaatc
      841 ccgtcaccgg ttgtccgctg acagcagcgc tcgcggcaat tggtgggaag tggaaaatga
      901 taatcgtcta ttggcttgct gaatccccaa aacacttcgc tgctttgcga cgcgccatgc
      961 cgggaatctc acaaaaggtc ctgactcaac agttgcgcga gctcgtgaac gacggcctca
     1021 ttgaacgtca gcctcatggc gtggttcccg cgaaggtcga atattcgctg tcagattatg
     1081 gccgttccgt tttaccttta attgaaaacg ttcgcgtttg gggccggaac catatggaac
     1141 ggccaggaga ttgacggttc gtttctgttt attaaaatga gatcagtaat cgttatactc
     1201 acgctatttt tgacgcttcc gatagctgcg caaacaaaac cgccggcagt ctatgttgat
     1261 cgcggagcct gtccatttga atgctgcacg taccggacct ggaagaccga caaagacacg
     1321 gttgcttatg ctcaaccaaa taagagctcg cgacgagttg gagttttaaa ggctcgtaca
     1381 aacgttgctg ctttgacagg cgaagtacga acatggcctg gcaaatttac aattatcaaa
     1441 gcgcacgata agtacaagcc tggagatgtg ttgtgggttt atacgccgct gggagaaggg
     1501 ttctataaag tttggttcaa aggacaaatg accgagcagg agctcgacta tatgagtggg
     1561 ccattcgaac aaactttccc acgttgcgaa gacgcgaagg actgttgggg tcaacttgaa
     1621 tcgccgctgc gggtcgaatg gtgggtgaag ataaagagca aggacggctg ggtgggatgg
     1681 accaacgaaa cagagaactt cagtaatcaa gacgcgtgcg ggtgagccgg actcagctct
     1741 gttcgatagt ccagggacgc attaacaatg gaccagaccc acaagacaat ctacatcacc
     1801 ttggtgctgt gcttcacggt agtcttccag ggctgctcaa gatcgtatcc tcagctcgac
     1861 tggtcagagt taacacctct ctgcaaacaa caggcgctta atgcaaccgc tctactaaca
     1921 tcagaaaaag agctggcaaa gatcgaagaa ctcgatgccg cactttacaa accaattgcc
     1981 ggtctaacga tctttctcga gtcacaaact ccgaccttcg acagcagaaa tttgaaaccc
     2041 cgctactggc tgcgcgtgga agactattcg agcactgcaa tcgcgtcgca gagagcgacc
     2101 gaatacagta gtgtcgatac atacgaaaga attgccaggg cttccggcgg agacagcttt
     2161 atagtttcga aaacaaccgt tcgactgtgg gcgatcgcgc gcggtaagcg agtttacgcc
     2221 ctcacgactg acacgaacct gttcggattg attgaaatgc cggttaaatt acgaaaaacc
     2281 attggaatgc tccccgaaag gtgaattttg tttcttgaga atcgcgattt caggaacgca
     2341 ttgttccggc aaaagtactc ttgccgaatc attcctcatg gaccatcccg cctatattca
     2401 tgaacccgaa gcgtacgaag cactcgggga gttctgcgga gaacatttct cagataaacc
     2461 aactcctggc gacctgttcc ggcaactcga atatcacgtt aagcgtctcc aacattacaa
     2521 ggaagccgat tgtgtaatat tcgagcgtgc tgttgtcgat tatgtggcct acatacaaat
     2581 actggaggag tttgccggct cggcactgga taaaaatcta acttccgacg ccatccagat
     2641 tgccaaagag cacataaaac ttcttgacgc cgtagtattt ctccccttgc atggttttgc
     2701 tggcgacgca ccggttgatg aagatttaag actgagacgt cgcgttgata gtcggctcga
     2761 ggctattttg ctgaatgatg agttggactt atttgttggc gctggtccac tattaatcga
     2821 agcgacggga actacgcaac aacgactggc ggatattaat aaacttcttg agttgaactg
     2881 actatgggca tcgtcgtctt tctaattacc tctgccgttc aattcctggt cgccgttttt
     2941 ggcttcttca ttctgctgtt ggcgctgaat ggctacagtg aacaatctgc cacgccaggt
     3001 atagttattt atacgctagt cagtctgatc atagtgcttg gcttggcttc catagccgca
     3061 ttcgtttcga caaggatcgt aagtagaaaa gggttaggct ccctggctgt aggttcgttg
     3121 agcaccatcg ggttttcaat acttggcgga gtctttctgg ttggaacttt cttcgtgtca
     3181 gtaggaatag ctgaactcct tcgcactcta aaatgaagca tcgattgtaa agaggacgaa
     3241 tcatgaatca acacggcgag cccaggataa ccgcaacatt gtctgtaagg aattggtcca
     3301 aggcaatggc gttttacaaa tctgccttcg gcgcagttga gttgtttagc gtccccggtg
     3361 gcggcgtggg tcaactgtca gtttccggcg ccgagttttg ggtcgcagag gaatcaccag
     3421 aacatctaaa cttcagccct gaaacactag ggggttgttc ggtgcgcatg ctgatgattg
     3481 tcgaagatcc agattcggtg tatcaacagg caattaacgc cggcgcaact gaggtagttt
     3541 ctgtgactga agaacatgga tggcgactcg gacggatcgt cgatccgttt ggacaccact
     3601 gggaaatcgc aaaaaaactt tcctaagaga gaagcccgac gtgcattctc cgtgggcgac
     3661 taattagtgt gagcatgcta aagaacacgg tttggaaccg acgcccgaac tgatggaaaa
     3721 agttaaggcg tcaatcgaag acgagcgcac agctcaagct tgagagataa atagcaggtg
     3781 cgcgacaaca tcatcagaaa cgtcgcgcac ctgtcgacgt gagcgagcaa ttaccgttta
     3841 ggttgctcgc tcgaatgcgc cggcaagata caacggttcc agacctgtgg cccaatattc
     3901 aagcgcggct cgatccggtt gggttgtttc acgtccggtg cgctttgatg gcaatgccgc
     3961 acttaacggc acaaagtcga tccatccttc ccatttgcca cccactcgtt cgccatagat
     4021 cagaacgtca tatacttgac cggcctcgtc ttgaacttga tcatcgtgtc ggcggatcaa
     4081 ctccggtgcc tccgcaactc gatcccatat aacgtctatc gatatctgct tctcgtctcg
     4141 aacgtgatat tggactctta tatcggcgta agccgggact tctatttgtt cgccagccag
     4201 ttctacatcg actgaacgat cttcttccaa acctctagct aaagcacgta aaagcgcagt
     4261 aacttccttt gatgtctctc tcatattctt agatggtcaa atggttagcc gtcggggcga
     4321 agcggaggag gtatctgaac agcagtctaa cagagtagtt ataacagttc tgaatcggat
     4381 atgttgcgat tcagtgattt aaagtcggcc atgcaactaa aacgatgctt gctataattc
     4441 gcggtgagct agattttgtc ggttgaacag ttttaacaat ggaccatagc accggatcaa
     4501 gacgcgcact ttggcaggta atcttctcgg tggtctattt atcctttttt ccaccgattc
     4561 tgttttggtt ggccggtgac tggcggtgga ttgaaggatg gatattctct gcgctcttgt
     4621 gtctacttag cctcgcaaca gttttctatt tgtacttgaa ggatccggct cttctcaatg
     4681 agcgctttgg ctcgccggtt caaaagaatc agaaatcgtg ggacaaagtc ctggtcgtac
     4741 tcttcatttt cgcgttccta acctggtatg caattatgcc gctggatgca cgacagttcg
     4801 aatggagtcc gcattttcca atttgggtaa atgtaattgg agctctcctc tttagctttg
     4861 gcttttatct tctctatcga gctatgagag agaacaccta cgctgctcca gtcataaaga
     4921 tgcaaaaaga gcgaggccag accgtcattg caactgggcc ttactcgatt gtgcgccatc
     4981 caatgtatac gggagggaca ctcgttttcc ttggcggacc cttactactt ggatccctct
     5041 acggtctgct tgccggattc ttactttcgt tgatgcttgc cttacgcact ttcggagaag
     5101 aagcgatgct ccggacggag ctgcctggat acgccgaata tcgtacacgc gtcaggtggc
     5161 gtctgatacc ttatctcttc tgaaaaacga gcttatgccg ctccctgttg cgcacgctct
     5221 ggtaggcgct accgtaaaaa cttcccttag cgaatctcat caagctcttt cgcttaaggc
     5281 tattagcttg actgttctcc ttgcgatctg tccggatttt gattacgccc taaactttct
     5341 gcagatcgga ggcggcggtt ggcatcatgg atttacgcat tcgatccttt tcgccatcgc
     5401 aatcgggcta ctttgttggc tcatacttaa ggataagcgc cccacaactc tctttattct
     5461 ttgcgcagtt gtcgtttcac atacactact ggactatctg tttaccgaat cccggggtgt
     5521 tgccctgttt tggcccataa cagattaccg gtacaaactt aattggttca acccgattaa
     5581 ctatagttgg cgaagcacct cgataagcgc agctgttgct gaccttgcac ggctttgtgt
     5641 actcgaactg cttatcttta gtcctgtttt gctgttggcg ctgttaatca aaaaacgctt
     5701 tgcctcaaag tgagttaatc atctggtcca catttcgaga ttctgttaca aacaattaga
     5761 atacctccgc ttctgtattt ccataaagga aacttcatgc gcccattaaa aaccgttctt
     5821 tctttttgct tactcttaac tgcaacaact caaccggtcg tcgcacaaac aacatccctt
     5881 gctgaacaag tgaagcgcct tcaggacgat ccgagtgtca aagcggccaa cgattacatc
     5941 gacaaacatc gcgatccgat cctcagcgaa tggattgcaa tcaccgagat caacgctccg
     6001 tcgaaacagg aacaggaacg tgcaaaa
//
LOCUS       DASSFP010000391         3847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_112749, whole genome shotgun sequence.
ACCESSION   DASSFP010000391 DASSFP010000000
VERSION     DASSFP010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112749"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2405)
                     /locus_tag="VFH91_08800"
     CDS             complement(<1..2405)
                     /locus_tag="VFH91_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6863135.1"
                     /translation="MSRALLFVAMVIAFSTVTLAQTSKGTVTGTVTDPNGAVIAGAEV
                     TLTSAETRLSRTTTTGTEGLYRFEAVDPGTYSLKVISVGFGDVVSTGVEVRANQTSDV
                     PAQMVPSGQVATVNVAADAGQLLQSEAPVRGGNIDKARITELPFAGRNPVSLALTLPG
                     VSTNRFGFGVGTFSVNGSRGRSNNFLIDGTENNDISVAGQAFQITNPDAVQEVSVQTS
                     NYDAEFGRAGGAVVNTITKSGTDDFHGSLAYLFDVTRDDAITNTQSLSAAIRERGHPP
                     RGTEQYYAGTLGGPLLIPHFGEGEPMFSYDGRRRTFFFVAWQEQFRSASTQASRTVLT
                     PAGRTALRNLFPLGVNPRVDLLMDVTKNVIGESAFQNIDIGARPGCPAPCNIQFGTGS
                     LFVPQTYRERQAQLRIDHKIGPNDEFSARYLFDDQKDPLGGGITFPGFGTANNNTYQN
                     LLFNETHIFSSSWTNEARLAYNRILIDFPLNPDDALGLVLPRFDISTLAQFGIATNLP
                     QGRVANNYVFQDTLTHIHGNHTFRFGLDLLEQRSRQFAPIVERGLLTYRSGGGFTALG
                     NYIDNFAGSGGGTQRDFGSPWYYPFLFRQAYFAQDRWRVSDSLTLTLGLRYENFGNAI
                     NTLRTAAFTGLFNVDPVTFTGPYSQPNEVDNDNNNFAPTFGLAYSPSYEHGWLARIFG
                     NRETVIRMGYQLGYDSFFNNIASNAATSSPNVVATAIPSAVNTSTPRGLPNVTGSLPL
                     TPRPLSPLDSQTLVVKNLVNPYYQRWSAGFQRALPGNLVIDASYVGSKGTKLYLNEDL
                     NPSV"
     gene            complement(2892..3152)
                     /locus_tag="VFH91_08805"
     CDS             complement(2892..3152)
                     /locus_tag="VFH91_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863136.1"
                     /translation="MYEVAVVTVGDKFIAVGFVISGAGTVRAFCRIRGADRQFMLIVV
                     VAVEGMKVSVMKIIRMPFVTNGGVSAVLAVFMRMTLMFFAAH"
     gene            3477..>3847
                     /locus_tag="VFH91_08810"
     CDS             3477..>3847
                     /locus_tag="VFH91_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6863137.1"
                     /translation="MKKADQLVLVIDDDPSMRMAITELIEAVGLSCQTFGSAKEFLGA
                     ELPDVPKCLVLDVRLPGLSGLNLQRELIERNIQIPIIFITGHGDIPMTVQAMKAGAVE
                     FLTKPFRDQDLLDAIEQATER"
BASE COUNT         1003 a          948 c         1012 g          884 t
ORIGIN      
        1 acacttggat ttagatcctc attgagatag agcttggttc cctttgagcc gacatacgac
       61 gcatcaatca cgaggtttcc aggtaatgcc cgctgaaagc cggcggacca acgctggtag
      121 tagggattaa ccagattctt gactacaagc gtctgagaat ccaacggtga cagcggccgc
      181 ggagttaatg gcaatgaacc ggttacgtta ggtaagcctc gtggcgtact ggtattgacc
      241 gccgagggaa ttgcggtagc caccacgttg ggtgaagaag tcgcggcatt cgaggcaata
      301 ttgttgaaga acgaatcgta tccaagttgg taccccattc ggatcaccgt ttcacgattg
      361 ccaaaaatcc gtgccagcca cccatgttcg tacgacggcg aataggcgag accaaacgtg
      421 ggagcaaagt tgttgttgtc gttatcgacc tcattaggtt gactgtatgg accagtaaac
      481 gtaactggat caacgttgaa tagacctgta aaagcagctg ttcgaagagt gttgatagcg
      541 ttaccaaagt tctcgtatcg caatccgagt gtgagtgtta atgagtcgga gacacgccaa
      601 cgatcctgcg cgaaatatgc ctggcggaac aggaacggat aataccatgg gctgccaaaa
      661 tctctctgtg ttccaccgcc tgaacctgcg aagttgtcga tgtaattacc gagcgcagtg
      721 aagcccccgc ccgatcgata ggtcaacaga ccacgttcga caatcggcgc gaactgccgg
      781 gaccgttgct cgagtaggtc caggccaaaa cggaaggtgt ggtttccgtg gatatgagtg
      841 agcgtgtcct ggaagacgta attatttgcc acgcgacctt gtggcaagtt cgtggctata
      901 ccaaactggg ccagcgtgga aatgtcgaac cgcggtaata ccagcccaag tgcatcatcc
      961 ggattaagcg gaaaatcgat caggatccgg ttataggcaa ggcgcgcctc gttggtccaa
     1021 ctggatgaga agatgtgcgt ctcattaaag agcagattct gataggtgtt gttgttggct
     1081 gtgccaaatc cgggaaatgt gattcctcct ccgagcggat ctttctgatc gtcaaataaa
     1141 taacgtgctg agaactcatc gttcgggccg atcttgtgat cgatacgcaa ttgtgcctga
     1201 cgctcgcgat aggtttgggg aacaaagaga gaaccggttc caaactgaat attgcatggc
     1261 gcgggacaac ctggacgagc tccaatatca atattctgga aagccgactc accaataacg
     1321 tttttggtca cgtccattag gagatcaact cgtgggttga cgccaagcgg gaacaggttc
     1381 ctcaaggccg tgcgtccggc cggggttaga acagttctgg aagcttgtgt tgaagcactg
     1441 cgaaactgct cttgccaggc gacgaaaaag aaagtgcgtc gacgaccatc gtaactgaac
     1501 atcggctcgc cttcgccgaa atgagggatc aaaagtggac cacccagtgt ccccgcataa
     1561 tactgttcag taccgcgggg tggatgacca cgttcgcgaa tcgcagccga gagagactgc
     1621 gtgttggtga tggcatcgtc gcgggtgacg tcgaagagat aggccaaact gccatgaaaa
     1681 tcatcggtac ccgacttcgt aatcgtattc acgacagcgc cgcctgctcg accaaactcc
     1741 gcatcataat tcgaagtctg caccgagact tcctgtaccg cgtcggggtt ggtgatctgg
     1801 aatgcctgtc cggccaccga gatatcgttg ttctcagtgc catcaattaa aaagttgttg
     1861 gagcgtccgc gggatccatt gacagagaac gtgcccacac cgaagccaaa gcggttagtt
     1921 gaaactcctg ggagtgttag agccagggaa accggattgc gtcctgcaaa tggcagttca
     1981 gtaattcttg ccttatcaat attgccgccg cgcacaggtg cttcactttg gagtagctga
     2041 ccagcatcag cagctacatt caccgtcgct acttgtcctg atgggaccat ttgggcggga
     2101 acatcagacg tttgatttgc tcttacttca acgcccgtgc ttacgacatc accaaagccc
     2161 acggaaataa ctttcagtga ataagttcct ggatcaactg cttcaaatcg atagagccct
     2221 tcagtgccag ttgttgtagt gcgactgagt ctggtttcgg cacttgtaag cgtaacttca
     2281 gcaccggcga tcactgcacc gtttggatca gttactgttc cggtcacagt gcctttacta
     2341 gtttgagcta aagtaaccgt tgaaaacgcg attaccatag ccacgaaaag cagagctcta
     2401 ctcattatgt gcctccgaag aaagtaaggt tgagcggttg tgtttacaag atttcgtata
     2461 acactcggcg ggcactcgga cccaaatgag aattggtcaa gcgtgccgaa ttcaatttat
     2521 gcaagcgcta ttccgatttt tgcagagagg cggaatcaga tgtaggtgta ggtggaggtt
     2581 agagaacggg actcgacgaa cctcaaatta aggcagatcc gattcagaat tctggacaga
     2641 aatctcttta atattggatt gctagcttaa acttgtttgc tgacatgtgg cgggatccag
     2701 caatgagaaa agcaatactc caaaatttcg tacgctcgct cttcagggaa aagaaattgt
     2761 ctctacgcat aacgctcctc tcatcccgaa agactggcca tactaagaca cgttagattg
     2821 cgcctgtgtg agtgccgctc tatagtctag ctgggttgtt tcagccccct tgtcatgcac
     2881 gaaaggagaa attaatgggc tgcaaagaac atcagagtca ttcgcataaa cacggcgagg
     2941 actgcggaca caccgccgtt cgtcacgaag ggcatacgga ttatcttcat gacggacacc
     3001 ttcattccct ccacggcgac cactacgatg agcataaact gtcggtcggc gccgcgaatc
     3061 ctgcagaatg cacgcacagt tcctgcgccg gagatcacaa atcccacggc gatgaacttg
     3121 tcccccacgg tgaccacagc gacttcgtac atggtggact cctacaccac cggcacggcg
     3181 atcactgcga cgaacacggg cggataaacg cctgagcccg acggagtttt tacaccagca
     3241 aggtttaagg caggtcgatt cgtctcgagc ctgccttaaa ctggcaaccg ctatgaagtc
     3301 agacctgacc aaagctcgct ttagcttttg actcccacta agtaagcgcc cattgtgatc
     3361 cactttcagc tgcatacgat catcgaagca cacgaggaag attttgggct acacagaacg
     3421 aggagaaagg agcgacaata cattattctt ccatctgaaa cagcgagggc cacatcatga
     3481 aaaaagcaga ccaactcgtg ctcgttatag atgatgatcc atcaatgcgc atggcaatca
     3541 ccgagttgat tgaagcagtc ggcctaagtt gccagacttt tggttccgca aaggagtttc
     3601 tcggagccga gcttccggat gtaccaaaat gccttgtgct tgatgtacga ctgcctggat
     3661 tgagtggact gaatcttcaa cgcgagctga ttgagcgaaa tattcaaata ccgatcattt
     3721 ttataacggg ccatggcgat atcccgatga cggtccaggc catgaaagca ggggcggtag
     3781 agtttttgac aaagcccttt agagaccagg atctgctgga cgctatcgag caggcaactg
     3841 agcggga
//
LOCUS       DASSFP010000392         5896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1004244, whole genome shotgun sequence.
ACCESSION   DASSFP010000392 DASSFP010000000
VERSION     DASSFP010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004244"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1053
                     /locus_tag="VFH91_08815"
     CDS             226..1053
                     /locus_tag="VFH91_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HET6863138.1"
                     /translation="MTDMTFGPYSTRLKTILLLAFCAGTLIFINCQPATTAPKTAMNL
                     PAPTYGYEIVHTYPHDPEAYTQGLIFKNGELLESTGRNGKSTLRRVDLETGKVLDRVA
                     VPEQYFAEGIALLNEKIYQLTWQHQLCFVYDHHTFKKVDQLSYDGEGWGLTTDGHSLI
                     LSDGTSRLRFLSPANFNVTRTLEVKDDKGPLTELNELEYVKGEIYANVWHEQRIAIID
                     PQSGKVTGWINLQGLLSPTEVSDEEGVLNGIAYDDKTDRLFVTGKLWPKLFEIRVKR"
     gene            1059..3215
                     /locus_tag="VFH91_08820"
     CDS             1059..3215
                     /locus_tag="VFH91_08820"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HET6863139.1"
                     /translation="MGFATYFRAFSYAMIGVAMISLLVAGGLSATLALVFLVVMVVSW
                     RLETTRWQVPEKIGLIIVLLSIPLFYVDWRYQQSIGEPLERLGVTALAHLIVFLSAVK
                     LLQVKKDRDWVFLYLISFFEVLLAAGLSFSPIFLVTLSLYLLSALSAVTAFEIQKARR
                     AVKLSETRLLVPPDSRVFKGKRNRVTRSSEAIRLPFVAVGLLVLIFILAIPLFLVAPR
                     SGAASLTRSSGGLSNFIGFSENVTLGQIGELKRDDRLVMRVRVEDSQSPKDLRWRGVA
                     LDEFTGRGWKKSIDSHNTDKRVSDRGGGGFFQFDTTEALHRLTTQTFFLEPIESPVLF
                     AAPRPVAIQGDLPFVRVDGEGSIQTRRHEFERILYKAISDTEEPRVDLLRADLRPYAP
                     AIYRYLQLPAGLDPRIGKLATRIINEADARDRYDAARAIEAALQREYTYSLLMKAGGD
                     DPLADFLFNVKAGHCEYFSTAMAIMLRTHGIASRVVNGFLPGEYNEASGAFSVRQSDA
                     HSWVEVYFPETKSWVTFDPTPPAGRAEPIRTGITAQLQKYAEAMELLWFQYIVGYDKQ
                     EQRTLAISLTSRVNNYGRIVTSMLNSARNYIPSNALELTVFSLGLLLFGTLMVLGYRV
                     FRLGWRRALRLSEDDERPRAGVAFYERLIKVMEERGVARDKAQTPMEFASSLRSTDAL
                     VITRAYNRVRFGNQKLSSHEMREVERALTELESTEN"
     gene            3257..4606
                     /gene="rimO"
                     /locus_tag="VFH91_08825"
     CDS             3257..4606
                     /gene="rimO"
                     /locus_tag="VFH91_08825"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET6863140.1"
                     /translation="MKKVGFVSLGCPKNLVDSEVMMGQLQQSGYQVTANAAEADTIVV
                     NTCGFIDAAKQESIDAILEAARLKTEGTAKRLIVAGCLVERYRDELKAELPEVDAFIG
                     TSQINEILTVCDPKTNTRTLPVVPLGNQTATYLYDESTPRVLATPSHYAFIKIAEGCD
                     RPCAFCFIPQMRGHFRSRRFGSIVAEAHQLAEEGVKELILVAQDSSRYGEDLGKQDAL
                     ARLIRELSRTDGIEWVRVMYTYPTHISDSFLDAIADEPKAVKYLDMPLQHASQNVLKL
                     MKRGGNRKSLERLIERVRKRVPGIAVRTTFITGFPGETDEDFNELMGFVKNVEFDRVG
                     VFTYSDEEGTPAFELAEKVPHKTAAKRRTSLMKEQARISRRNNKSRVGDTVRVVFEGE
                     SKESELLWQARMESQAPDIDGCILINDVPDGVVPSIGDFVNVQITEAHEYDLLGRIC"
     gene            4783..5364
                     /locus_tag="VFH91_08830"
     CDS             4783..5364
                     /locus_tag="VFH91_08830"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET6863141.1"
                     /translation="MLKESSDELNPEVGATDAVSPRHQPPRTLLDYVALALATFGVGY
                     LPIAPGTLGSLVGVLIFLAARRMALVYGIDALILEASLIIVITLLGIWAASETERLAG
                     RKDPGKVVVDEVSGQMISLFPLVLFRWWPVAPTVIISFILFRFFDIVKPYPARRFERL
                     RSGLGIMADDLVAGVYGAIVTVIGMYIFVSYVG"
     gene            5374..5538
                     /locus_tag="VFH91_08835"
     CDS             5374..5538
                     /locus_tag="VFH91_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863142.1"
                     /translation="MQESKKRQSEATSDETLEDLEEETSELNVGRDRSMPSPDGAFDE
                     EDELAEADPE"
     gene            complement(5689..>5896)
                     /locus_tag="VFH91_08840"
     CDS             complement(5689..>5896)
                     /locus_tag="VFH91_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET6863143.1"
                     /translation="LDPAFAPGVSHREPGGLSTRQAIALIQQLKGSVVGADIVEFNPR
                     MDPLHVTGTVCAKLLKEIAARMME"
BASE COUNT         1422 a         1408 c         1576 g         1490 t
ORIGIN      
        1 aattttcgag ccttcgtcgg tacgggtcgc gagtaggcaa gactttaagg atcccccaac
       61 tgccgttggg ggaatttcga gctttagtcg gtgtcgcgag taggcaggac tttaaatatc
      121 ccccaactgc cgttggggga attatgcttg ttcgattccc gagccccgcg atcaaatgaa
      181 aaatggggag gcttcctttt gtcttttcaa cgtgcgtcca gcaaaatgac tgatatgact
      241 ttcggcccat attccacaag acttaaaacc atccttttgt tagctttctg cgccggaact
      301 ctaatcttca tcaactgcca gcccgcaacc acggctccta agactgccat gaatctgcca
      361 gccccgacct acggctatga aatcgtccat acctatccgc acgatccgga ggcctacacc
      421 caggggctaa tttttaagaa tggagaatta cttgaaagta ccggcagaaa tggtaaatcc
      481 accctcaggc gagtcgattt agagacaggt aaagtgctcg accgagttgc tgtgccggaa
      541 cagtattttg ctgagggaat tgcccttttg aacgaaaaaa tctaccaatt aacctggcag
      601 caccaactct gcttcgtcta tgaccatcac actttcaaga aggtcgacca gctcagttat
      661 gatggcgagg gatggggatt aactaccgat ggtcattccc tgatactgag cgacggaacc
      721 agccgtctgc gatttttatc acctgccaac ttcaacgtca ctcggacact ggaagttaaa
      781 gacgataagg ggccgttgac tgaactaaac gagcttgagt acgtcaaagg tgagatttac
      841 gcaaacgttt ggcatgagca acggatcgcc attattgatc cacagagcgg aaaggtgact
      901 ggttggatca acctgcaggg cttattgtcg ccaacagaag tttcggatga agaaggtgtg
      961 ctaaacggca tcgcttacga cgataagaca gatcgtctat tcgttaccgg aaagctttgg
     1021 ccgaagttgt tcgagattag agtaaaacga tagaactgat gggcttcgca acgtatttcc
     1081 gagctttttc ctatgcaatg attggtgtgg caatgatttc attgctagtt gccggaggac
     1141 taagcgcaac acttgcactc gtattccttg tcgtcatggt tgtcagctgg cggctcgaaa
     1201 cgacgaggtg gcaagttccg gagaagattg gactcataat agttctgttg tccattcctc
     1261 ttttctatgt ggactggcgg taccagcagt ccattggcga gcctctcgaa cgactgggag
     1321 tcacagcgct cgctcatcta atcgtttttc tttctgcggt gaagttgttg caggtgaaga
     1381 aagatcgcga ctgggtcttt ctttacctaa tctcattctt tgaagtcctc ctcgcagccg
     1441 gactgagttt tagtccgata ttcttagtca cgctgtccct atatctctta agcgcgcttt
     1501 ccgcagtcac agcgttcgag attcaaaagg cgcgcagagc cgtaaagctg tctgaaactc
     1561 gcttgttggt tcctcctgat tcgagagttt tcaaaggcaa acgaaatcga gttacgcgtt
     1621 ccagtgaagc catccgactg ccatttgtag ctgttggttt actggttctg attttcatac
     1681 tggctattcc gctcttcctt gtagcaccta gatcgggcgc cgcaagtttg acgagaagta
     1741 gcggtggact ttctaacttc atcggctttt cggagaacgt gactttgggc cagatcggcg
     1801 aacttaaacg ggacgatcga ctcgttatgc gagtaagagt agaagattcc cagtcgccga
     1861 aagatctgcg ttggcgaggc gtggcattag acgagttcac cggccgcggt tggaaaaaat
     1921 cgattgattc ccacaacacc gacaagcgcg tttcagaccg cggcggcggc ggattctttc
     1981 agttcgacac gactgaagct cttcatcgtc tcacgaccca aacctttttt ctcgagccga
     2041 ttgagtcgcc cgttttgttt gcggcgccgc ggccagtagc gattcagggt gatctccctt
     2101 ttgtaagagt tgatggtgaa ggttcgattc aaacgcgtcg ccacgaattc gagcggatac
     2161 tctacaaagc aatctccgat actgaggaac cgcgcgttga tctattgcga gctgatctaa
     2221 gaccttatgc gccggcaatc tatcgttatc tgcaactgcc ggctggcctt gatcctcgaa
     2281 taggtaaact cgcaacaagg attattaacg aggctgatgc gcgtgatcgt tacgacgctg
     2341 cgcgggcgat tgaagctgcg ttgcaaaggg aatataccta ctcgttactc atgaaagccg
     2401 gtggtgacga tccgcttgct gacttcctct tcaatgtgaa agccggacac tgtgagtact
     2461 tttcgacggc gatggcgatc atgctgcgaa cgcatggtat tgcctcgaga gtcgtaaacg
     2521 gcttcttgcc aggtgaatat aacgaggcct ccggtgcgtt cagcgtccgc caaagcgatg
     2581 ctcactcgtg ggtcgaagtg tactttccgg aaacgaagag ttgggtcaca tttgatccaa
     2641 cgccgcccgc cggacgtgca gaaccaattc gcaccgggat aacggcgcag ttgcagaagt
     2701 acgccgaagc aatggagttg ctctggtttc aatacatagt tggttacgac aagcaggaac
     2761 aacggactct ggccatctct ctcacgagtc gcgtcaacaa ttatggtcga atcgttacgt
     2821 cgatgctgaa ctccgcacgt aattacatcc cgagcaatgc acttgagcta acagtgtttt
     2881 cgctgggcct gctattgttt gggactctga tggtccttgg ttatcgggtc tttagactgg
     2941 gatggcgtcg cgcattgaga ctctcagagg atgacgaacg tcctcgcgct ggagtcgcct
     3001 tctatgaaag attgattaag gtgatggagg agcgcggcgt cgctcgtgac aaagctcaaa
     3061 caccgatgga gtttgccagc agtttgagat cgacggacgc gctggtgata acccgagcct
     3121 acaaccgcgt tcgctttgga aaccagaagc tttcatcgca tgaaatgcga gaagttgagc
     3181 gtgctctaac tgaactcgag tcgactgaga attagtttta tatagatttt agaaagttaa
     3241 gtttgagatt tgtttaatga agaaagttgg atttgttagt ttgggttgcc cgaaaaacct
     3301 tgtcgatagc gaggtgatga tgggccaact acagcaaagt ggttatcaag taacagctaa
     3361 cgcagccgaa gctgatacca tcgttgttaa tacctgcggc tttatcgacg cagcaaaaca
     3421 ggaatcgatc gatgccattc ttgaagcagc ccgtctgaag accgagggaa ctgcaaaaag
     3481 actgatcgtc gctggttgtc ttgttgagcg ttatcgcgat gagcttaagg ccgagctgcc
     3541 agaggtggac gcatttatcg gcacgagcca gataaacgag atacttactg tctgtgatcc
     3601 gaagactaat acgcgaacgc ttcccgtcgt tcctttggga aatcaaacag ccacctatct
     3661 atatgacgag tcgacgccgc gagtattggc gactccttcg cattacgcct tcataaaaat
     3721 tgctgaaggt tgcgatcgac cttgtgcttt ttgctttatt ccacagatgc gcggccattt
     3781 tcgcagccgt cgctttggtt caatcgtcgc cgaagctcac caactcgctg aggaaggtgt
     3841 caaggagttg attctcgtgg cccaggattc gtcgaggtat ggagaggatc tcggaaagca
     3901 agacgctctt gcgcggctga ttcgtgagtt gtctcgcact gacggcatcg agtgggttcg
     3961 cgtgatgtac acctatccga ctcatatcag tgacagtttt ctcgatgcga ttgctgatga
     4021 accaaaggca gtcaagtatc tcgacatgcc gctgcagcat gcgtcgcaaa atgttttgaa
     4081 actgatgaaa cgtggcggta accggaaaag tctggagagg ctcatcgaac gtgtccgaaa
     4141 acgcgttccc ggtatcgcgg tacgtactac attcatcacc ggctttccgg gagaaacgga
     4201 tgaagacttt aacgagttga tgggcttcgt gaaaaacgtt gaatttgatc gagttggggt
     4261 ctttacgtat tcagatgaag aggggacgcc tgcattcgag ctcgctgaga aagtgcccca
     4321 taaaactgct gcgaagcgac gcaccagtct catgaaggag caagctcgca tttctcgacg
     4381 caacaacaaa tcaagagttg gcgacaccgt gagggttgtt tttgaaggtg agtcgaaaga
     4441 gagtgaacta ctttggcagg caaggatgga gagtcaggct ccagacatcg atggttgcat
     4501 tttgatcaat gatgttcctg atggtgtcgt tccttcgata ggtgactttg tgaacgtgca
     4561 gatcaccgaa gcacacgaat atgacctcct gggacggatc tgctaacttt ggagtgcgcc
     4621 ggcccggatt ggccactctc tgcgtcactc tgcggttctc tgcgtctctc tgcgtctttc
     4681 tgaacgggaa ccgaattggc ctgaaacaga ggatcgcaga gagacgcaga gaaccgcaga
     4741 gagacgcaga gaaatacagg gaagagatcg gcgcactcca aggtgcttaa agaatctagt
     4801 gacgaactga atccggaagt tggggcgaca gatgctgtgt cgccgcggca tcagccaccg
     4861 cgaactctac tggactatgt ggcactcgcg ctggcgactt ttggtgttgg ttatcttcca
     4921 attgctcctg gcactttagg ctcgctggtt ggagttttga tctttcttgc cgctcgccgc
     4981 atggccttgg tctacggtat cgacgcctta atcctcgaag cttcactcat aatcgtcatc
     5041 acgctgcttg gtatttgggc cgcatccgaa acagaacgtc tcgctggccg caaagatccc
     5101 gggaaagttg tggttgatga ggtctcgggc caaatgatct ctctgtttcc cctggtcctg
     5161 tttcgttggt ggcccgtggc tcctacagtg atcatttcgt ttattctttt tcgctttttc
     5221 gacatcgtaa agccgtaccc tgcgagacgg tttgaacgat tgcgcagtgg gctcggaata
     5281 atggccgacg acctggttgc gggtgtttat ggtgcaatcg tgacggtaat aggtatgtac
     5341 atttttgtca gctatgttgg ttaaggggga agaatgcagg agtcaaagaa aagacaatct
     5401 gaggcgacga gtgacgagac tctcgaagat ttggaagaag agactagtga attgaacgtg
     5461 ggccgtgacc gatcaatgcc gagtcctgat ggagcgttcg acgaggagga tgaactcgcg
     5521 gaggcggacc ccgaataggg gtcaggagtc acgagtcagg gggcggcgga attattggag
     5581 aattatcatt tgatatttga gaatttctct ttcgatccga cgagcacccc gtaaacaatt
     5641 aacaattctc aaatatcaaa tgataattct ccaatgagtc cgcgcgccct attccatcat
     5701 tcgcgcagca atctccttca acagcttagc gcatacagtc ccggtgacat gaagaggatc
     5761 catgcgggga ttgaattcga caatgtctgc acctaccaca ctgcctttca attgctgaat
     5821 taaggcgatt gcctggcgcg tagataatcc cccgggctcg cgatgagaaa caccaggcgc
     5881 aaacgccgga tccagc
//
LOCUS       DASSFP010000393         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1004414, whole genome shotgun sequence.
ACCESSION   DASSFP010000393 DASSFP010000000
VERSION     DASSFP010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004414"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFH91_08845"
     CDS             <1..263
                     /locus_tag="VFH91_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863144.1"
                     /translation="VGLSKAISKFTKANPEVFKFKAGVVEGKVVALNEVEAIASLPSK
                     EELISKVMFLINCQAQRLVTVISAVPRNLAIVIKQIGDQKSA"
     gene            508..897
                     /gene="rplL"
                     /locus_tag="VFH91_08850"
     CDS             508..897
                     /gene="rplL"
                     /locus_tag="VFH91_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET6863145.1"
                     /translation="MAATREDVIEFLKNMTLLEASQLVKDLEETFGVSAAAAAVAAPA
                     GGGVPSGEAAAPAEEKTEFDVILQNVGGNKINVIKVVREVTALGLKEAKDLVEAAPKA
                     VKEAVSKEEAETIKQKLSDAGATVEVK"
     gene            1253..>5140
                     /locus_tag="VFH91_08855"
     CDS             1253..>5140
                     /locus_tag="VFH91_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863146.1"
                     /translation="MSANLLQTINTGLGRRTSQAPERVDFSKIATAVRIPNLIEIQRE
                     SYNRFLQMDLLPEEREATGLQAVFQSVFPISDFRGTATLDFVEYQIGNWQCKCGRLEG
                     LNYLRANCKSCGSTIKVNPLAAGETLCHKCGTFNFVRPQLCENCGEPVGLKHKHDQQE
                     CQERGMSYSVPLKVKIRLTVYDKDPDTEVLSIRDIKEEEVFFGEIPLMTDNGTFIING
                     TERVIVSQLHRSPGVFFKRGALYVAKIIPYRGSWVEFEYDQKNLLYVRVGKRKFLASI
                     FLRALGLWFNQSEQKSFRTDSQLEEVVKGASFTDEDMLRSLYVTDAIRMEEGRLLLQV
                     PEEGSTNLIGMKVDFDIKGKGGDPIVRSGKKVTLSALDGLRKAKIGEVEIDSSQLEGA
                     FALSDIVNTESGEIVLEANNEITPAKLQEIVEAGITGFSVFFPERDDVGPVLSLTLKK
                     DVITKPVDALLEIYRKMRPGDPPTVQTAYRLLEAMFFDPRKFDFSRVGRLKFNIKMGK
                     PERNRIEDPLLSAEDFYEVVAYVLKMRKNPSEYQADDIDHLGNRRVRAVGELLENQFR
                     IGLERMERAIKEKMSIHQEMQTTMPRDLINAKPVTAAVREFFGSSQLSQFMDQTNPLS
                     EITHKRRLSALGPGGLSRERAGFEVRDVHPTHYGRICPIETPEGPNIGLISSLSCFAR
                     INEFGFIEAPYRKVTDGRVVEYVRVVNGGDTKLKPGDHVPQQDVDNANKKIPSDGRRA
                     EFEPWPFYLTAWEEDKHVVGQANIELGEEGNIINERNAARKAGEFILALRKDIEYVDV
                     SPKQLVSVAASLIPFLENDDANRALMGSNMQRQSVPLLRAEAPYIGTGMEKVTARDSG
                     AVVAAKRDGVVDYVDSERIIVKADHNVDGTISREVTADIYTLIKFKRSNQNTCINQRP
                     IVHVGERVEKGQVIADGPCTDRGELALGRNVLVAFMPWRGYNFEDAILVSERLVKEDY
                     YTSIHIEELEIEARDTKLGPEEITRDIPNVGENMLRDLDDSGIIRIGAQVKPGSILVG
                     KVTPKGETQLTAEEKLLRAIFGEKAGDVKDASLVSPPGIDGTVVDVQVFTRKGQEKDT
                     RSLSIEQEEEDRLRRDLEDEIRILREQRDERIYELLEGRKLSVDLTSNREVVIPKNVQ
                     ITREMLKEVEPKILRKVELASSRLDIGAEIKEYEDRTERQIKILSDIYEEKIAKLRQG
                     DELSPGVIKMVKVFIAMKRKLSVGDKMAGRHGNKGVIARILPEEDMPYLPDGTPVEIV
                     LNPLGVPSRMNVGQILETHLGWAARVLGLHFATP"
BASE COUNT         1340 a         1229 c         1386 g         1185 t
ORIGIN      
        1 ccgttggttt atctaaagcg atttcgaagt tcaccaaggc caatccagag gtctttaagt
       61 tcaaggctgg agtggtagaa ggcaaagtcg tcgctctgaa tgaggtcgaa gcgatcgcaa
      121 gtttgccttc aaaagaagag ctgatttcca aagttatgtt tttgatcaac tgtcaggcgc
      181 aaagattggt aactgtgatt tctgcggttc cgcgcaacct ggcaatcgtt atcaagcaga
      241 ttggcgatca aaagagcgcg taagaagttt gatttttttg ataatacgga tcagtttgtt
      301 ttggtcgttt ggtcaagaca agttggttta aacagtcagt gggccgacgc ggacaacgag
      361 cgcgacgata gtcaggtcgt cattaacggc tcggacgcct tttccaaact ccgatgcaag
      421 ggttttgaca aagcgtcccg tgggttcttc tgctgcgaac aacaacaaca gcacccagaa
      481 ccctttttag gaggatagaa agtaatcatg gcagcaacac gcgaagacgt aatcgaattt
      541 ttaaagaata tgacgcttct tgaggcttca cagctcgtga aggacctcga ggagactttt
      601 ggcgtttcgg ccgcagcagc tgcagtggct gcaccagcgg gcggtggcgt tccaagcggt
      661 gaagccgcgg ctccagctga agagaagact gaatttgacg taatccttca gaatgttggt
      721 ggaaataaga tcaacgttat caaggtcgtt cgtgaagtca ccgctctcgg gctgaaagaa
      781 gccaaggatc ttgtggaagc tgcgccaaaa gccgtaaagg aagcagtctc gaaagaagaa
      841 gctgagacaa tcaagcagaa gctttcagac gcgggcgcaa ccgtagaggt taagtagttt
      901 caaataataa tcgctcactc attcgcctga gctcgatgtc cgagaaatcg gcttcgacct
      961 cagggatgag tgttttggcc tttggccgct tgacagtcaa tttgttactg gctaagttaa
     1021 gggagtattg aggcgctggc gttactccca aaaaatttga tggaagagca ccgcttttgt
     1081 gtaacaagcg gtcggggcat tgtgcgctcc gaccgccatt tggcgtctcg ttaactttat
     1141 tgatggctag ttactgaaat ggcgaaatcc gacgggtcaa gttcgtacag gacgacccgc
     1201 tgaggattgt cggttccgta gttagcaccg ggcaatttat aaggaatctc acatgtcagc
     1261 aaatctgctt caaactatta atacgggatt gggaaggcgc acaagtcagg cgcctgaacg
     1321 ggtggatttc tccaagatcg caacagcggt ccgaatccca aatctaatcg agattcaacg
     1381 agagtcctac aataggtttc tgcaaatgga cttgttgccg gaggaacgcg aagccaccgg
     1441 gttgcaagcc gttttccagt ctgtctttcc gatttcagat ttccgcggca ccgccacgct
     1501 cgattttgtc gagtaccaga tcggtaactg gcagtgtaaa tgcggacgtt tggaaggtct
     1561 gaattatcta cgagccaact gtaaatcctg cggctcgact atcaaggtca atccactggc
     1621 agccggcgaa accctttgcc ataagtgcgg taccttcaac ttcgttcggc cacaactctg
     1681 cgaaaactgc ggagagccgg tcggtctgaa acacaagcac gaccagcagg aatgccagga
     1741 acgcggaatg agctacagcg ttcccctaaa agtcaaaatt agactaacgg tctacgacaa
     1801 agatccggat accgaagttc tctcgatccg cgacattaag gaagaagagg tcttcttcgg
     1861 tgaaatcccg ttgatgaccg ataacgggac cttcattatc aacggtactg agcgcgtgat
     1921 cgtttcacag ttgcaccgtt cgccaggtgt cttcttcaaa cgcggcgcac tctatgttgc
     1981 caaaattatt ccttatcgcg gctcgtgggt tgaatttgaa tacgatcaga agaatttgct
     2041 ttacgttcgc gtaggcaaac gaaagttcct cgcttccatt ttcctgcgag cgctgggctt
     2101 gtggttcaac cagtcagagc aaaaatcttt ccgcactgac tcgcaactgg aagaagtagt
     2161 taaaggcgct tcttttacag acgaggacat gctgcggtcg ctctacgtaa ctgatgcgat
     2221 tcgcatggaa gagggaaggc ttctgctcca ggttccggaa gagggaagta ccaacctcat
     2281 tggaatgaag gttgacttcg atattaaagg caagggcggc gatcctatcg ttcgaagcgg
     2341 taagaaagtt acgttgtcgg ctttggacgg actaaggaaa gcaaagattg gcgaagtcga
     2401 aatcgactca tcgcaacttg aaggagcgtt tgcgctttcg gacatcgtaa atacagagag
     2461 tggcgaaatt gttcttgaag caaacaatga aattacgcct gcgaagctac aggagatagt
     2521 cgaagccgga attacaggat tctccgtctt ctttcctgaa cgcgatgacg taggtccagt
     2581 tctctcgctc actttaaaga aggacgtgat caccaaaccg gtggacgcgt tgttagagat
     2641 ctaccgcaag atgcgtcccg gtgatccgcc gaccgttcaa acagcctatc gattgcttga
     2701 agccatgttc ttcgatccgc gaaagtttga tttttcgcgt gttggcaggc tcaagttcaa
     2761 catcaagatg ggtaagcccg aacgtaatcg aatcgaagat ccgctcctct cagccgaaga
     2821 cttctacgaa gtggttgctt acgtgctgaa gatgaggaag aacccgtcgg agtaccaggc
     2881 agacgatatc gatcatctcg gaaatcgtcg tgtgcgcgcc gttggcgagt tactcgaaaa
     2941 ccagttccgc atcggtcttg aaagaatgga gcgagccatc aaagaaaaga tgtcgatcca
     3001 tcaggaaatg cagaccacga tgccgcggga tcttattaac gccaagcctg tgacggcagc
     3061 agtccgcgag ttcttcggtt ccagtcagct ttcgcagttt atggaccaga ccaacccgct
     3121 gtctgaaatc acccataaac gccgcctgtc ggctcttgga ccgggtggtc tatcacgtga
     3181 gcgcgccggg ttcgaagtgc gagacgttca tccaacccac tacggtcgca tttgtcctat
     3241 tgagacgcct gaaggaccga acatcggttt gatctcgagt ctttcctgct tcgcgcggat
     3301 caatgagttt ggctttatcg aggcgcctta ccggaaagtg actgacggac gtgtcgttga
     3361 atatgtccgc gtcgtcaacg gaggtgatac caagctcaag cccggagatc acgtgccgca
     3421 gcaggacgtt gataatgcga acaaaaaaat cccaagtgac ggaagaaggg ctgagtttga
     3481 gccgtggccg ttctatctca cagcgtggga agaagacaaa cacgttgtag gccaggcaaa
     3541 catcgaactc ggcgaagaag gcaacattat caacgagcgc aatgctgctc gtaaggccgg
     3601 ggagttcatc ctcgcactgc gaaaagacat cgaatacgtt gacgtttcgc ctaaacaact
     3661 cgtgtccgtg gcagcgtcat tgattccgtt cctcgaaaat gacgacgcaa accgtgcgct
     3721 gatgggttcg aacatgcaac gccagtcagt cccccttctc agggccgaag ctccttatat
     3781 cggaactggt atggaaaaag tcactgcccg tgactcgggt gcggtcgtgg ctgcaaaacg
     3841 agacggcgtt gttgactacg tcgattcaga acgcatcatc gttaaggctg atcacaatgt
     3901 tgacggcacc atctctcgcg aggtgaccgc cgacatttac accctgatca aattcaagcg
     3961 ttcaaaccag aacacttgta tcaatcagcg accaattgtc cacgtcggtg aacgagtgga
     4021 gaaggggcag gttattgcgg atgggccttg caccgaccgt ggagaattag ctcttggacg
     4081 aaacgtcctg gtggcgttca tgccatggcg cggttacaac ttcgaagacg cgatccttgt
     4141 ctctgagcga cttgtgaagg aagactacta cacttcaatt cacatcgaag agctggagat
     4201 tgaggcgcgt gatacgaagt tgggccctga agaaatcacc cgggacatcc cgaacgtcgg
     4261 cgagaacatg cttcgtgatc tggatgatag cggcatcatt cgaattggtg cgcaggttaa
     4321 gccaggttcg attcttgtcg gtaaagttac tccgaagggt gaaactcaac tcaccgcaga
     4381 agagaaactc ttgcgagcta tcttcggtga gaaagccggc gatgtgaaag atgcttcgct
     4441 cgtcagccct ccaggcattg atgggaccgt tgttgatgtt caggtcttta ctcgtaaagg
     4501 tcaggagaaa gacacacgct ccctttcaat tgaacaggaa gaagaagatc gtctgcggcg
     4561 agaccttgag gacgaaattc gtattcttcg tgagcaacgc gacgaacgta tctacgaact
     4621 gttagaaggt cgcaagctct cggtggacct gacttcaaat cgcgaagtgg tgatacccaa
     4681 gaacgtacag atcacacgcg agatgttgaa agaagtcgaa ccgaagatct tgcgtaaagt
     4741 tgagctggct tcctccagac tcgatatcgg tgccgagatc aaagaatacg aagatcgcac
     4801 tgaacgtcag atcaagattc tctccgacat ttacgaagag aaaatcgcaa agctgcgaca
     4861 gggtgatgaa ctcagcccgg gcgtgatcaa gatggttaag gtcttcatcg ccatgaagcg
     4921 caagctttca gtaggcgaca agatggccgg acgccacggt aacaagggtg ttatcgcacg
     4981 catcctgcca gaagaagata tgccttatct tccagacggt acgccggtgg agatcgtgct
     5041 caatcctctg ggcgttccgt cgcgaatgaa cgtcggacag attctggaaa cgcaccttgg
     5101 ctgggccgcg cgagtacttg gtttgcactt tgcgaccccg
//
LOCUS       DASSFP010000394         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_715767, whole genome shotgun sequence.
ACCESSION   DASSFP010000394 DASSFP010000000
VERSION     DASSFP010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715767"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..>2219
                     /gene="leuS"
                     /locus_tag="VFH91_08860"
     CDS             358..>2219
                     /gene="leuS"
                     /locus_tag="VFH91_08860"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET6863147.1"
                     /translation="MLSVDLGDVMEEKYFPEQIEKKWQQRWEEAKAFEVSSEDPRQKF
                     YCLEMLPYPSGFLHMGHVRNYSIGDALAWFKRLQGFNVLHPIGWDSFGQPAEQAAIKR
                     GINPRDWTEQNIEHMKGQLQRLGISYDWSREIAAHRPDYYKWDQWFFLKMLERGLAYK
                     KVSQVNWCPNEHTVLSNEQSSGGICWRCGAQVVKRDLEQWFLRITHYAEQLLEDMKEI
                     DSAWPEKVLKRQRDWVGRSEGAYIDFAVKDSRQKIRVFTTRIDTIFGATAVVLSAEHP
                     LLDELLSESALKDDVKAFASKVKAERASADQTVEEQKEGLNTGILAINPFSGEIVPVW
                     VANYVLMEYGTGAVMSVPAHDERDFEFAQKYSLPIRQVVAPVTHQQAAAEPTSVTGTD
                     QSADMNRPFTDFGVLINSGDWGGKLSEVAIAEMSAYAKDKGFGEAAVTFRIRDWGVSR
                     QRFWGAPVPIIYCDKCGMVPVPYDQLPVVLPQNATFTGTGESPLAGVSEFVNTTCPKC
                     DGPARRDTDTMDTFVDSSWYFFRYCDPRNESMPFDSRIAQQWTPVDQYIGGDSHAVMH
                     LIYTRFWTKFMRDIGLVSFDEPVKSLMTQGMVTNRVQGGDEWKAMSKSLGNGVDP"
BASE COUNT          570 a          501 c          608 g          540 t
ORIGIN      
        1 gcctttacgt cgagttgcta cgcacccgaa gctagaccat tgtgccttta tcctcgagac
       61 gccttacgac gatccgcgcg ctgatattaa gaatctggag ttattgagag agtttgttgg
      121 gaagagccgg tgatcatttt caaaatgaaa aatgacaaat gctcggggat cgggttagaa
      181 tcccaacagt tgattacgac catgttttcg aaacgacctc gaactaaact cctcctaacg
      241 gtgccgcttc tgcgacgtgc ctaattgcgc gttttggcgg acagcggcga ttcccactcg
      301 acaagttatt acgtttagac gccaaaccgg gctggttttg tgcggtttag gcaaaacatg
      361 ttaagtgtag atttgggtga cgttatggaa gaaaaatact ttcccgaaca aattgaaaag
      421 aagtggcagc agcgctggga agaggcaaag gcctttgaag taagctccga agatccgaga
      481 cagaagtttt actgcctcga gatgttgcct tatccatccg gcttcttgca tatgggccac
      541 gttcgaaatt attcgattgg tgatgcgttg gcctggttca aaaggctgca aggtttcaat
      601 gtgctccatc caattggatg ggacagtttt ggtcagcccg ccgaacaagc ggcaatcaag
      661 cgaggcatca atccacgcga ttggaccgaa caaaatatcg agcacatgaa aggacagctg
      721 cagcgacttg gtatcagtta cgactggtcc cgcgaaatcg cggcacaccg tcctgattac
      781 tacaaatggg accaatggtt tttcctgaag atgcttgagc gaggtttggc ttacaaaaaa
      841 gtatctcaag tcaattggtg tccgaatgaa cacactgttc tgtcgaacga acaatcctcc
      901 ggaggaatct gctggcgttg tggtgcgcaa gtcgtaaaac gagatctgga acagtggttc
      961 ttacgcatca cgcactacgc cgagcagctt ctggaagata tgaaagagat cgattctgcc
     1021 tggccggaaa aggttttgaa gcgacaacga gactgggtcg gaagaagtga aggcgcctac
     1081 atcgactttg ccgttaaaga tagccggcaa aagattcgtg tcttcactac tcgcatcgac
     1141 accattttcg gcgccaccgc agtggttctg tcagcggagc atccattgct cgacgagttg
     1201 ctttcggaat cagcattgaa ggatgacgtg aaggcttttg cgtcgaaagt taaagctgaa
     1261 cgtgcaagtg cagaccaaac ggtcgaggag caaaaagagg gactcaatac agggattctg
     1321 gcgattaatc cgtttagtgg cgaaatagtt ccagtctggg tcgcaaacta cgtgctcatg
     1381 gaatatggaa ccggagctgt aatgagcgtt cccgcgcacg atgaacgcga tttcgagttt
     1441 gcgcagaaat actcgctgcc gatcagacag gttgtcgcgc cggtgactca tcagcaagca
     1501 gcagcggagc cgacttcggt tacaggaacg gatcagtcag ctgacatgaa tcgacccttc
     1561 actgattttg gagtgttgat taacagcggt gattggggcg gtaagttatc ggaagttgcg
     1621 atagctgaga tgtctgccta tgcaaaggat aagggatttg gcgaagcagc ggtcactttt
     1681 agaattagag actggggcgt ttcgcgtcaa cgcttttggg gcgcaccggt tccgattatc
     1741 tactgcgaca aatgcggcat ggtaccagtt ccttacgatc aacttccggt tgtcttgccg
     1801 cagaatgcga cgttcacggg aacgggtgag tctcctctcg cgggagtttc ggaattcgtt
     1861 aacaccactt gtccgaagtg cgatggtcca gcgcgacgcg ataccgatac gatggatacc
     1921 ttcgttgatt cctcgtggta tttctttcgc tactgtgacc cccgcaatga atcaatgccg
     1981 tttgatagtc gcatcgctca acagtggacg ccggtagatc aatatatagg aggtgactcg
     2041 catgctgtga tgcacctcat ctacacacgc ttctggacaa agtttatgcg cgacatcggg
     2101 ttggttagtt ttgacgagcc agtcaaaagt ttgatgacgc aaggcatggt cacaaaccga
     2161 gtacaaggtg gcgacgagtg gaaagcaatg tctaaaagtt tgggtaatgg agttgatcc
//
LOCUS       DASSFP010000395         8116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_421987, whole genome shotgun sequence.
ACCESSION   DASSFP010000395 DASSFP010000000
VERSION     DASSFP010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421987"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFH91_08865"
     CDS             complement(<1..920)
                     /locus_tag="VFH91_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6863148.1"
                     /translation="MSDVQRPNSSQLQIDPQQLDDISSRPPATTSHIEPSRDEVAVLT
                     WFEYFAFRWVRRMNEGRWKRFWTWCQMTMGAGWIHLSTYNIMRVYGLEHIEQASRERP
                     ILLVANHRSFFDMYAVSTVLFRSTSWRKQLFFPVRGRFFYQHPLGLLVNLIMGWWSMY
                     PPFFSTGDHPIAEKRPFDKYSFRVLTELARVGAGNVIGFHPEGTRNKNDEPYSFLRAQ
                     PGVGKLILDARPQVIPVFIAGLCNSLPRQIARNWNREELIRIHFGQQLDLAEYLEQPD
                     RLRTHKLIADHIMSKIAELGEQDRGLKAST"
     gene            complement(931..4443)
                     /gene="metH"
                     /locus_tag="VFH91_08870"
     CDS             complement(931..4443)
                     /gene="metH"
                     /locus_tag="VFH91_08870"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET6863149.1"
                     /translation="MSRFLDALKERIVVFDGAMGTSLQLQNLTLDDFGGLRFEGCNEN
                     LLVTRPDAVEKVHAGFLDVGCDVIETNSFNGTPVDFAEYGIGDKAYEMNVLAARLAKR
                     IASDYSTASKPRWVAGSMGPGRKLPTLGHISFNDLKAAYTEQVRGLLDGGVDLLIVET
                     CQDLLQTKAALVGIFEHFKATGSKVPVIAQVTIEVFGTMLNGTEISAALTSLAPFPID
                     LIGMNCGTGPRHMTESIRYLCENAPLPVSVLPNAGLPSVKDGKMHYDETPETFTEQLV
                     HFARDFGVNVVGGCCGTTPDHLKLVVEAMQSISPKRHDNQLVAAASSIYIQQPYVQDT
                     SFLIIGERVNASGSKKMRDLLNAEDWDGLVSLAREQEREGAHILDVNVDFVGRDGEKD
                     MHELASRLVTNVKIPLMFDSTEWQKMEAGLEHAGGKSLLNSTNYEDGEPRFLKVLELA
                     KRYGASVVIGTIDEQGMARTAEGKFQIAQRAYQQAIETGIPASDIFFDPLALPISTGI
                     QEDRRNALETIEGIRQIKKHLPGAFTLLGVSNISFGLNAASRVVLNSVFLHDAVEAGL
                     DAAIVNASKIEPLNRIGEDQLKVARQLIYDERRFDGDNCTYDPLTEFTKLFEGVKSKS
                     AVKVSKGETVEERLKNHIIDGEKIGLEEELSLALETHSALDIINNILLDGMKVVGDLF
                     GSGQMQLPFVLQSAEAMKAAVRFLEPYMEKKGGATAKGTMVLATVKGDVHDIGKNLVD
                     IILTNNGYKVHNLGIKQSIDSILQAYQEYGADAIGMSGLLVKSTLIMKENLELMNERG
                     INVPVVLGGAALTRRYVEDDLKSLYKGRLFYARDAFAGLHTMDKLVGGNGQASADEPD
                     VAVLDQAEDVEDLIGEEAKLGIRKPLKPRAIVQHKDTTHTQRSAVSEDVIIPAAPFYG
                     SRVVEDIPLDDVFAFVNETALFKGQWQFKQGRTPLEQYKELVDTKVRPILDDLKLKSQ
                     REHLLTPKVVYGYFPCQSEGNDLIVYHEDHKTERLRFTFPRQPNGKHLCLADFFASTT
                     SGKTDVVAFHLVTIGRRATEYAQELFKSDNYADYLYFHGLSVESAEALAELWHKRIRE
                     ELGIAGKDARELTKLFHQQYQGSRYSFGYPACPNLEDQQKLFSLLDPSRIDVNLTEEF
                     QLEPEQSTSAIIVHHPEAKYFSIE"
     regulatory      complement(4539..4613)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            4942..5439
                     /gene="coaD"
                     /locus_tag="VFH91_08875"
     CDS             4942..5439
                     /gene="coaD"
                     /locus_tag="VFH91_08875"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET6863150.1"
                     /translation="MRRAIYPGTFDPVTNGHLDIIERGCKLFDEIIIGILVNPDKHPL
                     FTIQERQEMLIEVLRDINKGNCELRVDSFRGLLVNYAVAQEADAIVRGIRAISDYEYE
                     LQMALMNRRLEPSIETVFMMPAEIYSYVSSRLVKEVFHLGGAIEGLVPPLIEKRMKEK
                     TPQQE"
     gene            5583..6785
                     /locus_tag="VFH91_08880"
     CDS             5583..6785
                     /locus_tag="VFH91_08880"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6863151.1"
                     /translation="MNTSPAMSPFPISQNVSRMQSSATLAAMQAAQALRAAGKDVFDF
                     GAGEPDFDTPDNIKRAAAEAMTAGRTKYTSTGGTREFHDAIIDFYRSTFNIEYQQAEV
                     MGTSGGKQAIFNAVVTLLDPGDEVLIPKPYWVTFPEIVVFAGGKPVYIETEETDFVLT
                     VEQVERAITPRTKLIILNSPSNPSGRVLPPLEFQRIVELAADRGLYVISDECYLRFVY
                     PPAEVFSAATLAPELRSRLCIAGSFSKTYAMTGWRMGYALGPIDWIRAMLKVQGHCTS
                     NPNSIAQYAAIEALRGPQESVGAMLAEYTARRGWLLNALQEIPGVKCFQPEGAFYAFP
                     DVRGCLKNGLKSSAEFSQRLLEEEHTVVTDGTGFGAEGYVRISYATSMTQLQEGIARI
                     KRFAEKHS"
     gene            complement(6863..>8116)
                     /locus_tag="VFH91_08885"
     CDS             complement(6863..>8116)
                     /locus_tag="VFH91_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6863152.1"
                     /translation="RDVAHEYELTWPEFKRDPFGFTKRTFVGYSQMLRKFLSNRNVVL
                     AMGAAVLGMFALVGAVALIDRSQSQQSSRVGLVVFAVIAAGLLVALFSTWLGRERGAA
                     VMGAEPSESRNVVIAMCSAFLFLFVIVGVIVAMDRRQKRQELLAQNAEELELQNLLDI
                     PEEQPTPDPGTAGLNKGSGGGSKPKPERAAGGGGGGREEQKPASFGKVPQASLEVPQV
                     VAPDPKPPKINNPSLPVPATVVADPALVPPDARVLPYGDYKSKSTDPSSGPGTGNGIG
                     TGTGGGIGPGEGGGLGPGRGGNIGGGDFNAGGGGPGGGGGGGDYNKIFTGKDVTTKAK
                     LISKPEPQYTEEARKNQVTGTVVLKVVFASSGQVTNIRTVSGLPNGLTERAIAAARQI
                     KFVPATKDGHQVSMWMQLEYNFNLY"
BASE COUNT         1946 a         2169 c         1972 g         2029 t
ORIGIN      
        1 ttagtcgaag cttttaggcc gcgatcctgt tcacccagct ctgcaatctt cgacattatg
       61 tggtcagcaa tcaacttgtg agtgcgtaaa cgatcaggtt gttccagata ctctgcaagg
      121 tctagttgct gtccaaaatg aatgcgaatc aattcctcac ggttccaatt tcgcgcaatc
      181 tgacgaggta gactgttaca caacccggca ataaagaccg gaatcacctg cggccgcgca
      241 tcaagtataa gctttccgac tccaggttga gctcgaagga acgagtacgg ctcatcgttc
      301 ttgttgcgcg ttccttcagg atggaagcca atcacgttgc cggcgcctac tctcgccagt
      361 tcagtcaaga cgcgaaacga gtatttatcg aacggtcgtt tctcagctat cgggtgatcg
      421 ccagtggaaa agaatggagg gtacatggac caccagccca tgatcaggtt gacgagcaga
      481 cccaggggat gctgataaaa gaagcggccc ctcactggaa agaagagttg tttacgccaa
      541 ctcgtcgagc gaaagaggac cgtagaaacc gcatacatat caaagaagga tcgatgattg
      601 gcaaccagca ggattggacg ctcccgagat gcttgctcga tatgttccag accgtagacg
      661 cgcattatgt tgtaggttga aaggtgtatc catccagccc ccattgtcat ttggcaccag
      721 gtccaaaagc gcttccaacg accttcattc attcgtcgca cccagcgaaa agcgaaatac
      781 tcgaaccacg ttaggacagc gacttcgtct cgtgatggtt cgatgtggga ggttgttgcg
      841 gggggacgcg acgagatgtc gtccaactgt tgagggtcga tctgcaattg actcgaattt
      901 ggtctctgaa cgtcggacat tgaaacccgg ctattcgatc gagaagtatt tagcctccgg
      961 atggtgaaca ataatagccg aagtggattg ttcaggctct agttgaaact cctccgtcag
     1021 atttacatcg atccgcgagg gatccagaag cgaaaacaat ttttgctggt cctccaaatt
     1081 cgggcacgca ggatatccaa agctatatcg agatccctgg tactgctgat ggaatagctt
     1141 cgtcaactcc ctcgcatcct tgccggctat cccaagctcc tcacggattc gcttatgcca
     1201 cagttccgcc agagcttcag cactctctac gctcaagcca tggaagtaga gatagtctgc
     1261 gtagttatcc gacttaaaaa gctcctgggc atattcggta gccctacgcc ctatggtcac
     1321 aagatgaaag gcgacgacat ctgtttttcc tgatgttgtc gacgcgaaaa agtcagcgag
     1381 gcataagtgc ttaccgtttg gctgtcgcgg aaaagtaaac ctcaaacgct ccgtcttatg
     1441 gtcctcgtga tagacaatca aatcgtttcc ttcagactgg caaggaaaat aaccataaac
     1501 aactttcgga gtgagcaaat gttcccgttg cgacttcaat ttcaagtcgt caagaatcgg
     1561 ccttaccttc gtatcgacca actctttgta ctgctcgagc ggtgtgcgcc cctgtttaaa
     1621 ctgccactga cctttgaata acgctgtttc gttgacgaaa gcaaacacgt catcgagagg
     1681 aatgtcttct acgactctgg agccatagaa cggcgccgct ggaattatga catcctcact
     1741 aacggctgag cgttgcgtat gagtcgtatc cttgtgctgg acaatcgctc gcggtttgag
     1801 tggcttacga attccgagct tggcttcttc tccgattaga tcttcgacat cctctgcctg
     1861 gtctaataca gccacatccg gttcatctgc gctggcttgt ccatttccgc caacaagctt
     1921 gtccatagtg tgaagtcccg cgaacgcatc gcgagcgtaa aacaatcggc ccttatagag
     1981 ggatttcagg tcgtcttcga cataacggcg agttaacgcg gcgccgccca gaaccacggg
     2041 aacgttgatc ccgcgctcgt tcattaactc gagattctct ttcattatca gagtggactt
     2101 taccagaagt ccgctcatac caatcgcatc ggctccgtac tcctgataag cctggaggat
     2161 gctatcaatc gactgcttga tcccgagatt gtgcaccttg taaccattgt tggtgagaat
     2221 aatgtccaca agatttttac ctatgtcgtg cacatcaccc tttacagtcg ccaacaccat
     2281 tgttcctttt gctgtggcgc cgcctttttt ctccatgtaa ggttccagaa accggactgc
     2341 agctttcatt gcttcggcag actgcagtac aaacggcaac tgcatttgcc cgctcccgaa
     2401 gagatcgcca acgaccttca tcccgtccag aaggatgttg ttaatgatgt cgagagctga
     2461 atgcgtttcg agtgcgagtg agagctcttc ttcgaggccg atcttttctc cgtcgatgat
     2521 gtggttctta agtcgctctt cgacggtttc acctttagaa accttgacgg cacttttgga
     2581 cttaacgcct tcgaagagct tcgtaaactc tgtgagcgga tcgtaagtgc aattatcgcc
     2641 atcgaatctg cgctcgtcgt agatcaactg ccgggcgact ttaagctgat cttctccgat
     2701 tcggttcagt ggttcaatct tgctggcatt gactattgcc gcgtccagac ccgcttccac
     2761 tgcgtcgtgg agaaataccg aattgaggac gacgcgcgaa gctgcattaa gcccgaacga
     2821 gatgttcgac actccgagaa gcgtaaacgc gcccggcaaa tgctttttaa tttgtcgaat
     2881 gccctcgata gtttcaaggg cgttccgtcg atcctcttga attcctgtag agatcggcag
     2941 cgccagtgga tcaaagaaga tgtcagatgc cggaattcct gtttcaatcg cttgctgata
     3001 agctcgctgg gcaatttgaa acttaccttc agcagttcgg gccatcccct gttcgtcgat
     3061 ggtaccgatt acgacactcg cgccataacg cttagccaat tccagaacct taagaaatcg
     3121 tggctcaccg tcttcataat tcgtagagtt cagcaaagat ttgccgcccg catgttcaag
     3181 gccggcttcc atcttctgcc actccgtcga atcgaacatc agtggaattt tgacgttcgt
     3241 aaccaggcgc gaggcgagct cgtgcatgtc tttctcacca tcgcgaccaa caaagtcgac
     3301 gttcacgtcc agaatgtgtg ctccctctcg ttcctgctca cgagccaggg atacgagacc
     3361 atcccagtct tctgcattga gtagatcgcg catcttcttc gaaccgctgg cattgacgcg
     3421 ctcaccaatg atcagaaaag aagtgtcctg gacgtatggt tgttgaatat aaattgagga
     3481 agcggccgct acgagttggt tgtcatggcg cttcggcgaa atactctgca tcgcctcaac
     3541 caccaacttc agatgatctg gagtagttcc acaacaacca ccaactacat tcacaccaaa
     3601 atcgcgagcg aagtggacca actgctccgt aaaagtctcc ggcgtttcgt cgtaatgcat
     3661 tttcccgtct ttaacagacg gcaggccggc gtttggcaac actgaaaccg gcaagggcgc
     3721 gttctcgcaa aggtaccgga tgctttcggt catgtgccgc ggtccagtac cgcagttcat
     3781 gccaatcaag tcgattggga acggtgcaag tgaagttaag gccgctgaaa tctcggttcc
     3841 gttgagcatc gtaccgaaaa cttcgatggt cacttgagct atcaccggaa ctttggagcc
     3901 agtcgccttg aagtgttcaa agattccaac cagggccgcc ttggtctgga gcaaatcctg
     3961 acaagtctca acgattaaaa gatcgacgcc gccatccaat agaccgcgta cttgttcggt
     4021 atacgcagcc tttaaatcat taaagcttat gtgacccagc gtcggtaact ttcttccggg
     4081 tcccattgag ccggcaaccc aacgcggttt gctggcagtt gaatagtcgg aagcgattcg
     4141 cttcgcgagt cttgctgcca gcacattcat ttcgtaggcc ttgtcgccta tcccatattc
     4201 ggcaaagtca actggagtgc cgttgaacga gttggtctca attacgtcac aacctacgtc
     4261 taaaaagcct gcgtgtacct tttcgacggc gtcgggacga gtcacgagca gattctcgtt
     4321 gcagccttcg aagcgtagac cgccgaagtc gtccagcgtc aggttctgaa gctgaagact
     4381 tgtacccatt gcgccgtcga agacgacaat gcgttctttt aaagcgtcga ggaagcgaga
     4441 cataagatat ttgccgagtt ctggtccgta gctgcgctgc tggtcagctg taaatattta
     4501 aggccccgcg ctaaacaacg cggagccttt gaaccttcta aaatcgactc acagctgttt
     4561 agcggattat ttttcgtggc cgcaagtcgc cttaactcac cacgtcacat gtcaaagcgt
     4621 attgaaacat tgggggtgga acctgtcaag cgacgtgttg tcgccggctg ccagcggccg
     4681 tcaatcactt ttcctttatt ttccatgtat cattttccat ttctcatttt tcatttctca
     4741 ttttccatct catttgcatg agccctccgg cgcgcaccca tttatctgct cgataaccga
     4801 gaaggcttag gaaatgaaaa atgaaaaatg gaaaatgata tatggaaaat gattgggaag
     4861 ctgatttaga aaatacctcg cttccctccc aacaacccct tgcgctttac catttcccca
     4921 atagccttta ccatttggcc catgcgacgc gctatctacc ctggtacatt tgaccccgta
     4981 accaacggac acctcgacat cattgagcgc ggctgcaaac tctttgatga aattattatc
     5041 ggaatcctgg ttaatcctga taagcaccct ctctttacca ttcaagaacg tcaagaaatg
     5101 cttatcgaag tcctccgcga tatcaataaa ggtaattgtg aactgcgcgt ggattcgttc
     5161 agagggttgt tagtcaatta tgccgtggcc caggaagcgg atgcgatcgt acgcggtatc
     5221 agggccatct ctgactatga gtacgaattg caaatggcgc tcatgaaccg gcgtctcgaa
     5281 ccatcgatcg aaactgtttt catgatgccc gctgaaatct attcatacgt tagttcgcgt
     5341 cttgtaaaag aagtctttca ccttgggggc gcaatcgaag gactggttcc acccctgata
     5401 gaaaaaagga tgaaagagaa gaccccgcaa caggaataat agaacctttt gttagatttc
     5461 cagcgtgtta tactcaagtc gaccagttat aagtctaaat tttcagatca acagctcgcg
     5521 tcaagaaagg aagtgcggat gagatcgcgc gattctgtga tttcatcacc tgacgtaatc
     5581 gaatgaatac cagcccagct atgtctcctt ttcctatttc tcaaaacgtt tcccggatgc
     5641 aatcatcggc aacgctcgct gctatgcaag cggctcaagc tctgcgcgcc gcaggaaaag
     5701 acgtttttga cttcggtgcg ggcgagcccg actttgacac gcccgacaac attaagcgcg
     5761 cagcggccga ggcaatgact gccggaagaa cgaaatacac ttctacgggc ggcaccaggg
     5821 aatttcacga cgccatcatc gatttctacc gctccacttt caatatcgaa taccaacaag
     5881 ccgaggttat gggcacttct gggggcaaac aggcgatttt caatgcggtt gtgactctgt
     5941 tggaccccgg tgatgaggtt ctgatcccaa aaccatattg ggtgacgttt ccggagattg
     6001 tcgtttttgc aggtggtaaa ccggtctata tcgagactga agagacagat tttgtactaa
     6061 cagtcgagca ggttgaaaga gcgataactc cacgtaccaa gctcattatt cttaactctc
     6121 catccaatcc ttctggtcga gtgttaccac cccttgaatt ccaacggatc gtcgaactcg
     6181 ccgcagaccg tggcttatat gtgatctccg acgagtgcta tttaagattt gtttatcctc
     6241 ccgcagaagt tttcagcgca gcaacgctgg cgccggaatt gcggtcccga ctctgtattg
     6301 ccgggtcgtt ctctaaaact tatgcgatga ccgggtggcg catgggctac gcgcttgggc
     6361 caattgactg gatccgcgcg atgctaaaag ttcagggaca ctgcacctcg aatccgaatt
     6421 caattgcaca atacgccgcg atcgaggctt tgcgcggacc acaagaatcg gtgggagcca
     6481 tgcttgccga gtacacagcg cgtcgcgggt ggctgctcaa cgctctacag gaaattcccg
     6541 gagttaaatg ctttcagccc gagggcgcgt tctacgcgtt cccggatgtt cgtggttgtc
     6601 tgaagaatgg gctgaagagt tctgcagagt tctcgcaacg actgctggaa gaagagcata
     6661 ctgtcgtgac tgatggaacc ggattcgggg ccgaaggata cgtgaggatc tcgtatgcca
     6721 cttcgatgac gcaactgcaa gaaggaattg cgcggattaa acgcttcgct gaaaagcatt
     6781 cctaatcgag ggtttcgagt ttacgaaaaa gcggcgctca aattgtttga gcgccgcttt
     6841 tatatttttc ttgacgcgat tcctaataca gattgaagtt gtactccagc tgcatccaca
     6901 tggacacctg atgtccatct ttcgtagccg gaacaaactt gatctggcgg gcagctgcaa
     6961 tcgccctttc agttaaacca tttggtagcc cggaaactgt tcgtatgttc gtgacctgcc
     7021 ctgacgaggc aaatacgact ttcaaaacca cagtaccggt tacctgattc ttacgtgctt
     7081 cttctgtata ttgcggctcc ggttttgaga tcaattttgc ttttgtcgtt acgtctttgc
     7141 cggtgaagat tttgttgtaa tcaccgccac cgccgccgcc acctggtcca ccaccgccgg
     7201 cattgaagtc gccgccgcca atgtttccac cgcgccctgg tccaagaccg ccgccttcac
     7261 cgggaccaat acctccgccg gtaccggttc ctatgccgtt gccagtccca ggccccgaag
     7321 acggatcagt actcttcgat ttgtaatcac catatggaag caccctggca tctggtggca
     7381 ccagagccgg atctgcaact acagtcgcag gtacaggaag ggatgggttg ttgatcttag
     7441 gtggctttgg atctggagct acgacctgcg gtacctccaa tgaagcctgt ggaaccttac
     7501 caaacgaagc tggtttctgt tcttccctac cgccgccacc acctccggcc gctctttcag
     7561 gtttcggctt acttccgcca ccacttccct tatttagacc ggctgtgcct ggatctggag
     7621 tgggttgctc ttcgggaata tccagcagat tctgcagctc gagttcttcg gcattctgag
     7681 ccaacaactc ctgccgcttc tggcgcctgt ccatcgcgac gatgacaccg acgattacaa
     7741 aaaggaacaa gaaagcgctg cacatcgcga ttacaacatt tcgcgattcg gaaggttccg
     7801 ctcccatcac tgcagcaccg cgttcacgac cgagccacgt tgaaaatagc gcgacgagaa
     7861 gtcccgcggc tatcaccgca aagaccacga gaccgacacg agaactctgc tgcgactgcg
     7921 atcgatcgat gagcgcgact gcaccgacca gcgcaaacat accgagcacg gcggcgccca
     7981 tcgcgagcac gacattacgg ttcgaaagaa atttgcgtag catttggctg taaccgacga
     8041 aggtgcgctt cgtgaagcca aatggatcgc gcttgaactc gggccacgtt aattcgtatt
     8101 catgggccac gtcacg
//
LOCUS       DASSFP010000396         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_557618, whole genome shotgun sequence.
ACCESSION   DASSFP010000396 DASSFP010000000
VERSION     DASSFP010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557618"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VFH91_08890"
     CDS             <1..175
                     /locus_tag="VFH91_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET6863153.1"
                     /translation="SEKIRTYNFKENRVTDHRIGLTVHQLDLVMEGNLDPFIDALVAH
                     YQAEKLKAEAVAA"
     gene            203..838
                     /locus_tag="VFH91_08895"
     CDS             203..838
                     /locus_tag="VFH91_08895"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6863154.1"
                     /translation="MPLATQVLLIRHGQSEGNAEKRFGGHTATPLSPLGRRQAEATAA
                     AISNESITAIYSSDLQRATATAEPLSVLTKIEINHSTAFRERGVGVMEGLTFQEAAEK
                     HPDQYAALLRRDFDHVLTGGESYRQLLDRAWMKLDAVVTEHAGGRIVIFSHTGTICIL
                     ALHLMGALDAPALKPVWISSSNCGISRFELRDDGFVRVLSINDTRHLIGIQ"
     gene            complement(840..1319)
                     /locus_tag="VFH91_08900"
     CDS             complement(840..1319)
                     /locus_tag="VFH91_08900"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HET6863155.1"
                     /translation="MSRVWRGLRWLMLVVVVLFMAAQFVRPAKTNPSSEQSLSIENQT
                     QVSPAVASIFDRSCNDCHSNKTRWPWYSNVAPVSWFVISHVNEARGDMNLSEWGKLDV
                     NRQRHRLQDMCEMVESGEMPLSSYTPFHPGSKLSQADVKTICDWTASERQRLSASPQ"
     gene            complement(1312..1479)
                     /locus_tag="VFH91_08905"
     CDS             complement(1312..1479)
                     /locus_tag="VFH91_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863156.1"
                     /translation="MSDLLGRRRFLYTMSAPALAFRTIGLTAWRLTSREPTNTENGIV
                     KCLLLAKGIYE"
     gene            complement(1513..2709)
                     /locus_tag="VFH91_08910"
     CDS             complement(1513..2709)
                     /locus_tag="VFH91_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET6863157.1"
                     /translation="MFSNHVTRDASAYCHGELSVDEGKKFAEHILICSKCRSEFDEIK
                     RGIAFATQLPIVEAPDSMWSKLERQLAQVSNSSYALKTHRVFLTRTQLAVIAASLLLC
                     SVVAIIWYRRVNINAINQPFWQVTNLDGIPTIASKGIGNTGRLSVGQWLETDNKSRAQ
                     ISVGSIGHVEIDPNTRVRLLETDPTAHRLELAEGKLSARIWAPPRLFFVDTPSAIAAD
                     LGCAYTLEVGKDGSSMLRVTSGWVELQLKDRESMVPAGAACETRPGIGPGTPYFEDCS
                     TNFRNQLVSIDFGEGRDNRSLAIINIVNESRPRDTLTLWHLLSRVDGDDRVRVLEKIE
                     SFVPMPAGVTREGILRLDPEMLARWRTALEGTWQGNFTTPKNFAESYWKIKNGVTRRM
                     SNFGSR"
     gene            complement(2749..>3087)
                     /locus_tag="VFH91_08915"
     CDS             complement(2749..>3087)
                     /locus_tag="VFH91_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6863158.1"
                     /translation="LVVNACMDERRRRKRLLTIEVNVADVPGAGKASQETQYAKNEVA
                     AAVRSAIKELKPKFRLPILLKYIEGLSYEEIALVMGCSKGTVASRLNRGHSQLAKRLA
                     HLSEHAEITD"
BASE COUNT          763 a          830 c          715 g          779 t
ORIGIN      
        1 atcggaaaag atcaggacct ataacttcaa agaaaaccgg gtgaccgatc accgtatcgg
       61 attgaccgtt caccaactcg atctagtaat ggaaggaaat ctcgatcctt tcatcgatgc
      121 actcgttgcc cattatcaag ctgagaaact aaaggccgaa gcagttgcgg cttaactgac
      181 cgactttact cgtcgatcct ggatgcccct agctactcaa gttcttctga ttcgtcatgg
      241 ccaatcggaa ggaaacgctg aaaagcgttt tggtggtcat acggcaactc ctctttcacc
      301 acttggccga aggcaggcag aagctacggc agccgcgatt tctaatgagt caattaccgc
      361 catctactca agcgatctcc agcgcgctac tgccaccgcc gagcctttat ccgtcctaac
      421 gaaaattgaa ataaaccact cgactgcgtt tcgcgaacgc ggcgtcgggg tgatggaggg
      481 tttaactttt caagaggccg cagaaaagca cccggaccag tacgccgcgt tactgcgccg
      541 agactttgat cacgttttga ctggcggaga gagttatcga cagctacttg atcgcgcctg
      601 gatgaaactc gatgccgtcg tgacagaaca tgccggagga cgcatcgtga tcttcagtca
      661 cacgggaacc atctgcatcc tggccttgca tctgatggga gcgttggacg cgccagcact
      721 caaaccagtc tggatttcga gctctaactg tggaataagt cggtttgaac tgcgcgacga
      781 cggctttgtc agggtacttt ccatcaacga cacccgacat ctcatcggta ttcaatagat
      841 cattgcggcg aggctgatag tctttgacgc tcgctcgcgg tccagtcgca gatagtcttc
      901 acgtccgcct ggcttagctt tgaaccagga tggaatggcg tgtaagagct caaaggcatc
      961 tcaccactct caaccatctc acacatatcc tggagtcggt ggcgctggcg attcacatct
     1021 aatttccccc actccgaaag attcatgtcg cctcgtgctt cgttaacatg actgatcacg
     1081 aaccatgaga ctggagcgac attcgagtac cagggccagc gagtcttgtt cgaatgacaa
     1141 tcattgcacg atctgtcaaa gatagacgca acggctggac tgacctgggt ttgattttcg
     1201 atgctaagtg actgttccga ggaaggattt gtttttgccg ggcgcacaaa ctgcgcagcc
     1261 atgaatagaa caactacaac taacatcaac catctcaacc ctcgccagac tctactcata
     1321 gattcctttc gccaacaata agcactttac gataccattt tccgtattgg ttggctcacg
     1381 actcgttagc cgccaggctg ttaatccgat tgtccgaaaa gctaaagcgg gtgcggacat
     1441 cgtgtacaag aaacgtctgc ggcccaatag atctgacatg atggtctcct ttcgaccagc
     1501 ttggtccagc tctcatcgac taccgaaatt agacattcgg cgtgtcacgc cattcttaat
     1561 tttccaatac gattcggcaa agttcttagg cgtcgtaaaa ttcccttgcc atgtcccttc
     1621 gagagcggtt ctccaacgcg cgagcatctc cggatcaaga cgcaaaattc cttcgcgagt
     1681 aacgccagct ggcatcggca caaaactttc aatcttctct aaaactctta cacggtcgtc
     1741 accatcaacc ctggaaagca aatgccagag tgtcagcgta tctcgcggtc gcgactcgtt
     1801 aactatgtta attatcgcga gtgatctgtt gtctcgtcct tcgccaaaat cgattgagac
     1861 caactgattg cgaaaatttg tagaacagtc ctcgaaataa ggcgttcctg gaccaattcc
     1921 gggcctcgtc tcgcaagcgg ctccggcagg gaccatcgat tcccggtcct tcagctgtaa
     1981 ctcaacccat cctgaggtca ctcgcaacat gcttgaacca tctttaccaa cttcgagagt
     2041 gtaagcacac ccaagatcgg ccgctattgc tgagggagtg tccacgaaga aaagtcgagg
     2101 tggcgcccag attctcgcgc taagtttacc ctccgccaat tccagcctgt gcgctgtcgg
     2161 atctgtttcg agcaaacgca cgcgcgtatt gggatcaatc tcgacatgtc ctatcgagcc
     2221 aactgaaatt tgcgccctcg acttattgtc agtttcgagc cattgaccta cgcttaaacg
     2281 gccagtgttc ccaattccct tcgacgcaat tgttggaatg ccgtcaagat tcgttacttg
     2341 ccagaacggc tgattgatgg cgttgatgtt tactcgtcga taccaaatga ttgcgacaac
     2401 agaacaaaga agaagcgatg cagcaataac cgcgagttgg gttcgcgtca gaaaaacgcg
     2461 atgagtcttt aacgcataac ttgaattcga gacctgcgcg agctggcgct ctaacttgga
     2521 ccacattgag tctggagctt ctacaattgg aagctgcgtc gcaaatgcaa ttccccgttt
     2581 gatctcatca aactcactcc tgcatttcga acagatcaag atgtgctcag cgaacttttt
     2641 accctcgtca acagacagtt cgccatgaca ataagccgat gcgtcccttg tgacgtgatt
     2701 cgaaaacata taactcttgc tttctggtgc ttagtttatt gggtcaattt aatcagttat
     2761 ttctgcgtgc tcactcaaat gggccagccg ttttgctaat tgcgagtggc ctcggttcag
     2821 acgcgaagcg acagttcctt tggaacatcc catgaccagg gctatttctt cataagagag
     2881 gccctcgatg tatttcagta aaattggtag cctaaatttt ggtttcaact ccttaattgc
     2941 agagcggacc gctgcggcaa cctcgttttt tgcatattga gtttcttgtg atgccttacc
     3001 ggcgcctgga acgtcagcga cgtttacttc tatcgttagc aagcgtttgc ggcgacgtcg
     3061 ttcatccata cacgcattga ccacaag
//
LOCUS       DASSFP010000397         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1149284, whole genome shotgun sequence.
ACCESSION   DASSFP010000397 DASSFP010000000
VERSION     DASSFP010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149284"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1544
                     /locus_tag="VFH91_08920"
     CDS             189..1544
                     /locus_tag="VFH91_08920"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6863159.1"
                     /translation="MARKNILVVDDEKAQREILELILTEEGYDVTTAASGEAAIKFAK
                     DRRFDLALTDLKMTGMDGIELLQHLLAFDSSIIVILLTAHGSIESAKEALRRGAFDYL
                     EKPYDKNALLETINRALDRLDAIDSEIISASPKMETVKKMILKVARSNSTVLVRGESG
                     TGKELIARAVHNQSPRSSEMFQAVNCAAINENLLESELFGHEKGSFTGAHAEKKGLFE
                     IADRGTLFLDEIAELDVGIQAKLLRALQERKIRRVGGIHEISVDVRVIAATNRDLRAM
                     VADGRFRDDLYYRINVLSIDVPPLRERREDIPVLIDFFLKKHTRNTTHAIRGLTSETR
                     KLMVDYSWPGNVRQLESAIERAILLCEGDEITAEDLPTEVRQDTGPAPEGTFKLPSEG
                     INFEDVERNLITQAMEQTDYNITKAAKLLGLTFRTLQYRLEKFGIRKPDGDGTGEEEE
                     E"
     gene            1673..2617
                     /locus_tag="VFH91_08925"
     CDS             1673..2617
                     /locus_tag="VFH91_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET6863160.1"
                     /translation="MKNISLTLILSVLFLPACSKSQDARLAENPALATQSAKVSEQAM
                     NSVAAKTQGSGYMNVSLDDASRAENSLQAANRRVLRNAEMTLEVPSTIETQHTVSVIA
                     ESHGGFVVNSESRQRENVEPAKRTVDIKLVVRVPSDQFGATIDQIRQLAVNLPTESLK
                     SDDVTEEFIDLEAHAKTQKALELQFLEIMKQAKKVEEALEVQRQLADVRAEIERIEGR
                     KRFLENRAALSTITVNLQSPTPIVVSSTGFGRSIRDAASDSLDVATGLVLFFVRSVIV
                     MLPVVVLVILPLALVFRFLVRRAKRMRLAQALATPTSN"
     gene            2705..3232
                     /locus_tag="VFH91_08930"
     CDS             2705..3232
                     /locus_tag="VFH91_08930"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HET6863161.1"
                     /translation="MNKIEALTVIGFLLVVLSSCSKPAVDQPRSSSAPATSPAKISSV
                     AVVKVNADEVDVSVGGSAEAKVRLNIDSGYHVNANPPTFSYLKPTELAINSKPPISVG
                     STIYPGATTRKFQFADKPLAVYEGETVIKVQLKVDKGASQGKQNLSGKLRVLACDEEV
                     CYPPGERVIMISVNI"
BASE COUNT          902 a          776 c          835 g          735 t
ORIGIN      
        1 acggggacag ggttaggact cgcgattgtg aaaaaagcca ttgacgatca caatgggcgg
       61 ataagcgttg catctaagga aggcactggc actacgttta caatgatttt gcccgtacat
      121 gagaaaggag aaatggtgaa tacggagggt tgattaaccc tcgttccctc aaatttcatc
      181 tcgcatctat ggctcgaaag aacatcctgg tagtagacga cgaaaaagcg cagagagaga
      241 tccttgaact gatcttaact gaggaaggtt atgacgtaac cactgctgct tccggcgagg
      301 ccgcgatcaa gttcgcaaag gatcgacgat ttgatctggc tctgaccgat ctaaaaatga
      361 ccggcatgga tggaattgag ttgctccaac atctattggc attcgattca tcgattatcg
      421 taattctgct caccgcgcac ggttcgatcg agtcagccaa ggaagctcta cgacgcggtg
      481 ccttcgacta tctcgaaaaa ccttacgaca agaatgcgct gcttgagact attaaccgcg
      541 cactcgatcg tctcgacgca atcgacagcg agatcatttc agcttcgcca aagatggaga
      601 cggtgaagaa gatgatcttg aaagtagctc ggtccaactc aacggtgctg gttcgaggag
      661 aatctggaac cggtaaagag ttgattgcca gagcggttca taatcagtcg cctcgctcaa
      721 gcgaaatgtt tcaggcagtc aactgcgctg ccatcaacga aaacctgctc gagtcggaac
      781 tgtttggaca tgagaaaggc tcgttcacag gagcccacgc tgaaaagaag ggtttgttcg
      841 agatcgcaga tcgtggaacg ttgtttctgg atgaaatcgc cgaactggat gtcggaattc
      901 aggccaagtt actgagagcg cttcaagagc gaaagatcag acgagttgga gggatccacg
      961 aaataagcgt ggacgtcagg gtgatcgctg cgaccaatcg ggatttacga gcgatggtcg
     1021 ccgatggacg ctttcgtgac gatctctact atcgtatcaa cgtgctctcg atcgatgttc
     1081 caccactgcg cgaacgccgc gaagatattc ctgtgctgat cgatttcttc ctcaagaagc
     1141 acacccgcaa caccactcat gcgatacgtg gcctaacaag tgaaacaagg aagttgatgg
     1201 ttgattacag ttggcccggg aacgtacgtc aactcgagtc agcaatcgag cgcgctattc
     1261 tgttatgcga aggcgacgag atcactgctg aggatttacc tactgaagtc cgacaagata
     1321 cgggaccggc gcctgaaggg actttcaagc tgccatctga aggaatcaac ttcgaagatg
     1381 tggagcggaa ccttataact caggcaatgg agcagaccga ttacaacatt acgaaggccg
     1441 caaaactact gggtctgact tttcggacac ttcagtaccg gctggaaaaa ttcggcatca
     1501 ggaaaccgga tggcgacgga acaggagaag aggaagaaga gtaattaaac tggtgatggc
     1561 cctaagctga agtgtgggaa ctcacgtacg caaagtatga gacgcgatgc aagactgttc
     1621 caccattcgc gttctacttt cgtaatcccg atatttcagg aggtcaaaac cgatgaaaaa
     1681 catctcgtta actctcatcc tttcagtcct ctttttgccc gcttgcagca aaagtcagga
     1741 tgctcgtttg gcagaaaacc ccgccctggc cacacaatct gcaaaagtca gtgagcaagc
     1801 aatgaatagt gttgcggcga aaactcaagg ctccggttac atgaacgtgt ctctcgacga
     1861 cgccagtcgc gccgaaaact cgctgcaagc ggccaaccga agagtgttac ggaacgctga
     1921 aatgaccctc gaagtacctt ccaccattga aactcaacac acagttagtg tcattgccga
     1981 atcgcacggc gggttcgtcg tcaattcaga atccaggcag cgcgaaaatg tcgagccagc
     2041 caaaaggact gtcgatataa aactagtggt ccgagttccg tcggatcaat ttggcgctac
     2101 tatcgatcaa atccgtcagc tcgcagtcaa tctgccgacg gaaagcttaa agagtgatga
     2161 cgtaacagaa gaattcatcg accttgaagc tcatgcaaaa actcaaaagg cattggagct
     2221 tcagtttctc gagataatga agcaggctaa aaaggttgaa gaggctctcg aggtacaacg
     2281 acaactcgca gatgttcggg ccgagataga aagaattgaa ggacgaaaac gtttcctgga
     2341 aaaccgcgcg gccctctcca ctatcaccgt caacctgcag tcacctaccc cgatcgtagt
     2401 cagcagtacg ggcttcggaa gaagtattcg tgacgccgca tctgatagtt tagatgtcgc
     2461 gacaggtttg gtgctgttct ttgtgagatc agtaattgta atgcttccgg ttgtcgttct
     2521 ggttatatta ccgcttgcct tggtatttcg atttctggtc cggcgggcaa aaagaatgcg
     2581 actcgcgcaa gctttagcaa cgcccacgtc aaactgaaca catacgcgga ttgtcgggac
     2641 gcgtcaggct attaccatgg cgagtaagat aggtgacacc aactccaact gaggcgcatg
     2701 tacattgaac aaaattgaag ccttaaccgt aattggattt ctcttggtgg ttttatctag
     2761 ttgttcgaaa cctgcggtgg atcagcctcg cagctcatcg gcgccggcaa catctcccgc
     2821 aaagatttcc tcggtggctg ttgttaaagt caacgctgat gaagttgacg tgagtgtcgg
     2881 tggctcggct gaagctaagg tacgactaaa catcgatagc ggttaccacg taaacgctaa
     2941 cccgccgacc ttttcatatc ttaaaccaac agagttggcg attaattcga agccgccgat
     3001 ctcggtcggc tcaacaattt acccgggcgc aaccacacga aagtttcagt tcgcagataa
     3061 gccgctggct gtttacgaag gtgagacagt catcaaagtc cagctcaagg tggacaaagg
     3121 agcttcacaa ggaaagcaga acctttctgg caaattacgc gtcctagcgt gtgatgaaga
     3181 agtatgctac ccgccaggtg aacgcgtcat catgatttcg gtaaatatct agtgaaaatc
     3241 ttttggat
//
LOCUS       DASSFP010000398         4454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_423942, whole genome shotgun sequence.
ACCESSION   DASSFP010000398 DASSFP010000000
VERSION     DASSFP010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423942"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFH91_08935"
     CDS             <1..414
                     /locus_tag="VFH91_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6863162.1"
                     /translation="SQIRIVRSYENGAPQVYGNAGKLQQVFTNLILNARDAIPDGGQI
                     TLATGTADDGSLMVEVADTGIGIDPENVAKIYDPFYTTKGVGRGTGLGLAVSYGIIQE
                     HTGRISVESTPGKGTTFRITLPTARARARLQAVGD"
     gene            572..1999
                     /locus_tag="VFH91_08940"
     CDS             572..1999
                     /locus_tag="VFH91_08940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6863163.1"
                     /translation="MKPERRNARKGSVLVVDDEEIMREVLETLLNAEGYRVDLAKTGE
                     EGLEAYGRRSFDVVLLDVSMPGIGGLRALEEFLKMDSEAVIVMITAYATFDTAIAAWE
                     RGAFGCIRKPFQNEQITATIAAGIKRRRKEEERRTLRRAMSKAVERGEIIGRSDLMQE
                     VFRLVEQVAPARSTVLITGESGTGKELIAKAIHEASPRAGRPFVTVNSSNIPSELLES
                     ELFGHTRGAFTGAVAAKKGLFEVADGGSIFLDEIGDIPPETQVRLLRVIQEREFTPLG
                     DTTPRRVDVRIIAATNIDLKEAVRQGGFREDLYYRLAVVPIELPPLRDRLEDILPLVQ
                     HFTQKYNDENGRTVSEQIAPEVLALLEGYSWPGNVRELENTIERAVVIAPGDEISREC
                     LRPEISDPRSSAAASQSGASNAAVGDIGRGINFYEEVRRFEIDLIRRALEQTSGHQSR
                     AARLLGMNATTLNSKIKTYNINLRP"
     gene            2035..2502
                     /locus_tag="VFH91_08945"
     CDS             2035..2502
                     /locus_tag="VFH91_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6863164.1"
                     /translation="MKRRELDGCPITGALQMLGDKWTMLVVRELASGPKRTMELLNSL
                     FPISSRTLVARLRDMENDNFIDRTDFGGNPPHIEYALTLRGSLLLPLLDSLRQLGLAL
                     GCNDCHDRRDRQGTYCESCPFNQYSRELEKPPLNQRSSQSPSRRQQDDSIILL"
     gene            complement(2512..3618)
                     /gene="trxB"
                     /locus_tag="VFH91_08950"
     CDS             complement(2512..3618)
                     /gene="trxB"
                     /locus_tag="VFH91_08950"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET6863165.1"
                     /translation="MDKETIKRDVVILGSGPAGLTAAIYAGRANLKPLLIDAPVDHVK
                     QTTPGGQLMITTDVENYPGFADGIQGPELMEEFRKQAERFGAEFLEEWVTEIDLSERP
                     FLLYTGNGTISAQTLIIATGASAKWLGIPGEAKVPDGFGGHGVSACATCDGPLPTFRN
                     RTLVVVGGGDTAMEEATFLTRYAARVFVVHRRDKLRASKIMQDKAFKNEKIKFIWNTG
                     VEEILGTPDSGVTGVRLRDLKTGVLETFSCEGVFIAIGHKPNTDLFKGQLDMDDVGYL
                     KTSGHSTATNIPGVFACGDVQDSVYRQAITAAGTGCMAAIDAERFLDHLPVAMPSGEE
                     VTMEGEHITADHTAIITPDGHMLPNQTEVGVKSE"
     gene            3794..>4454
                     /locus_tag="VFH91_08955"
     CDS             3794..>4454
                     /locus_tag="VFH91_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET6863166.1"
                     /translation="MSIQTSGSQTIETIAVIGAGTMGHGIAQVAGASGYKVFLHDIDR
                     DMLARGVQAIEKNLAKGIQLGKLTEDDMDRTLQHIHGTTSLEDCTAADLIIEAAPEKL
                     ELKRSILSELESVSDHTFIFASNTSSLSITEIARSSKRPDLVIGMHFFNPVHIMRLLE
                     IVVGISTSESTVQAVADVGRRMRKEPIIVKDVPGFASSRLGVALGLEAMRMLEQGVAS
                     AR"
BASE COUNT         1075 a         1121 c         1174 g         1084 t
ORIGIN      
        1 agccagattc gcattgtccg ctcatatgaa aacggagcgc cgcaggtata tggaaacgcc
       61 gggaaactcc agcaagtctt tactaacctg attctcaatg cgcgagacgc gattcctgat
      121 ggcggtcaaa tcactctcgc gacaggtaca gcagatgacg gttctttaat ggtagaagtt
      181 gctgatactg gtatcgggat agatccggaa aacgtcgcta agatttacga tccgttctac
      241 accactaagg gcgttggacg cggaactggt ctgggcctgg cagtctctta cggaataatt
      301 caggaacaca ccggcaggat tagcgttgaa agcacaccgg gtaaaggaac aactttccgt
      361 atcactctac caacagcgcg agctcgtgct cgattgcagg cagtcggcga ttgaacctgc
      421 atcgcctata attgatgcaa acagcgaacg aaatttgact attcgactca agacagtttg
      481 tattcggtta aggcctgccg ctgtcaggcc ttttgttttg tcagactaac tcgttaaact
      541 aaacatacta ttctgatgcc caatggattt gatgaagcct gaaagacgaa atgcccgcaa
      601 gggttcagta ctcgttgttg acgatgaaga gatcatgaga gaagtgttgg aaacacttct
      661 caatgcggaa gggtatcgag ttgacctcgc aaaaacaggt gaggaaggtc tcgaagccta
      721 tggccgccgc tctttcgatg tggttttgct ggacgtgtcg atgccgggca ttggtggact
      781 gcgtgcgctc gaagaattct tgaaaatgga ttctgaagcc gtgattgtga tgatcactgc
      841 ttacgcaact ttcgataccg ccattgcagc atgggagcgc ggcgcgtttg gctgtatccg
      901 caaaccattc caaaacgaac aaatcactgc gaccattgct gccggcatca aacgacgccg
      961 taaagaggaa gaacggcgca ctctgcgtcg cgcgatgagc aaagccgtcg aacgcggcga
     1021 aatcattggt cgctctgatt taatgcaaga ggtgtttcgg cttgtggagc aagtcgctcc
     1081 tgcccggtct acagttctaa ttaccggaga gagcgggact ggtaaagagt taattgctaa
     1141 agctatacac gaggcaagcc cacgcgccgg cagacctttc gtcacagtta atagttcgaa
     1201 tattccgtca gaacttcttg aatcggagtt gttcggacat acgcgtggtg cctttacggg
     1261 agcggttgcc gcgaagaaag ggctatttga agttgccgat ggaggctcga tttttcttga
     1321 cgagatcggt gatattccgc ctgaaacaca ggtgcgactg ttgcgcgtca tccaggagcg
     1381 agaatttaca cctctgggag acacgactcc gcgaagagtg gatgtgcgca tcattgccgc
     1441 cacaaatatt gatctaaaag aggctgtccg tcagggaggt ttccgagagg atctctatta
     1501 caggctcgct gtcgttccga ttgaattgcc tcccttaagg gatcgtctcg aagacatcct
     1561 gcctttggtc caacacttca ctcagaagta caacgatgag aatggccgta ctgtctccga
     1621 gcagatcgct cccgaagtgc ttgctttgct cgagggctat tcctggcccg gtaacgttcg
     1681 agaactggaa aacacgatcg aacgtgctgt tgtcattgcc cccggagacg agatcagtcg
     1741 cgagtgtttg cgtccggaga taagcgatcc acgttcctca gctgcggcgt cgcaaagtgg
     1801 ggcgagcaac gcagccgtag gagacatcgg acgtggtata aacttctacg aagaagtcag
     1861 acggttcgag atagatctaa tcaggcgagc gcttgagcag acgagtggcc accagtcacg
     1921 ggcagcgcgt ttgctgggca tgaatgcaac gacgctcaat tcgaagatca agacttacaa
     1981 tatcaatcta cgtccataag tacggacctt tgatctgaag agacttccca aaaagtgaaa
     2041 cgtcgcgaac ttgatggctg cccgattacc ggtgcattac aaatgctcgg tgataagtgg
     2101 acgatgcttg tggttcgcga gttggcttct ggtccaaagc ggacaatgga actcctcaac
     2161 agtctgtttc cgatcagtag tcgtactctt gtcgcgcgtt tgcgcgatat ggagaacgat
     2221 aactttattg accggacgga ctttggagga aatcctccgc acatcgagta cgctttaacg
     2281 ttgcgcggat ccttgctgtt gccgctgttg gattcgttac gccaacttgg gctcgcgcta
     2341 ggctgtaatg attgtcatga tcgtagggat aggcaaggta cgtattgtga gtcgtgccct
     2401 tttaatcagt attcacgcga gttggaaaaa ccgcccttaa atcagcgttc gtctcagtca
     2461 ccgtctcgcc ggcagcaaga cgactcgata attctactgt agtaaaacgc tctactcgct
     2521 tttgacacca acttcagttt gatttggaag catgtgccca tcgggagtaa tgatggcagt
     2581 atgatctgcc gtgatgtgct ctccttccat cgttacttct tcacctgagg gcattgcgac
     2641 gggtaggtgg tctaaaaacc gctcagcatc tattgctgcc atacatcccg tgccggctgc
     2701 ggtgatcgct tgccggtaca ccgaatcctg aacatcaccg caagcaaata cgccaggaat
     2761 attcgtcgcc gtcgaatgac cagacgtctt taaatagcca acgtcatcca tatccaactg
     2821 gcctttaaat aagtcggtgt tgggcttatg tccaatcgcg ataaatactc cttcgcacga
     2881 aaatgtctcg aggacgccag tcttcaagtc gcgcagtcgt acacccgtta cgcctgagtc
     2941 cggggtgcca agaatctctt cgactcctgt gttccagata aacttgatct tctcgttctt
     3001 aaacgccttg tcctgcatga tctttgacgc tcgaagttta tcgcgacgat ggaccacgaa
     3061 aaccctcgct gcataacgag tcaggaacgt tgcttcctcc atcgccgtgt cgccgccgcc
     3121 aacgactacg agagtcctgt tgcgaaatgt tggtagtgga ccatcacacg tggcgcaggc
     3181 cgaaaccccg tggcctccga acccatctgg aaccttggct tcacccggga tcccgagcca
     3241 tttggccgaa gctccggtag caataatcag tgtctgggcg gagattgtgc cattgcccgt
     3301 atacagcagg aatggccttt cgcttaggtc gatttctgtt acccactctt ccagaaactc
     3361 tgcgccgaag cgctctgctt gtttccggaa ctcttccatc aattcaggtc cctgaatgcc
     3421 gtcggcaaag ccgggataat tttcgacgtc ggtggtgatc atgagttggc cgcccggggt
     3481 ggtttgctta acatgatcca ccggcgcgtc tatgagtaat ggcttcagat tggctcgtcc
     3541 ggcgtagatt gcagcagtca atccggcggg tccagatccc agaatcacca cgtctctttt
     3601 gatagtctct ttatccatta ccttgatctg ttctccttca gcgggcccta agcccttgac
     3661 tcgtaattat ttgggatcgc aagcacgaga gtatagcctt ttcggttagc cgtatagtat
     3721 aactaccgat accactgtag tcgaaccaga ggttttggct gttaactcct atgcgaaacc
     3781 cggttccata agaatgagta ttcaaacaag cggttcccaa accatagaaa caattgccgt
     3841 gatcggcgcc ggtacgatgg gccatggcat agctcaagtt gccggcgcat ccggttacaa
     3901 agtatttctg cacgacatcg atcgagacat gttggctcga ggtgtgcagg cgatcgaaaa
     3961 aaacctggcc aagggaatcc aactggggaa actaactgaa gatgatatgg atcgcaccct
     4021 gcagcacatt catggcacaa ccagcctgga agactgcacg gctgcggatc tgattattga
     4081 agctgccccg gaaaagcttg agctgaaacg aagtatattg agtgaactcg agtctgtttc
     4141 ggatcacaca ttcatttttg ccagcaacac gtcgtcgctg tcgataacgg aaatcgcgag
     4201 gtcttcaaag cgccccgacc tcgttatcgg gatgcatttt ttcaatcctg tgcatatcat
     4261 gcgactgctc gaaatcgtgg tcggcatcag taccagtgaa agcacggttc aagccgtcgc
     4321 ggacgtgggc cgaaggatgc ggaaggaacc aatcatagta aaggatgttc ccgggtttgc
     4381 ttcgagccgg ctgggagttg cacttggttt ggaagcgatg cgtatgctcg aacaaggtgt
     4441 agctagcgcc cgtg
//
LOCUS       DASSFP010000399         5499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1006893, whole genome shotgun sequence.
ACCESSION   DASSFP010000399 DASSFP010000000
VERSION     DASSFP010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006893"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            559..1488
                     /locus_tag="VFH91_08960"
     CDS             559..1488
                     /locus_tag="VFH91_08960"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET6863167.1"
                     /translation="MTLAVEHFETRLTPGSVEANIGNTPLIRLRLAASDLPDDVGIFA
                     KAEQFNPGGSIKDRAALSMILDGERTGRLRPGMNIVDATSGNTGIAYAMIGASRGYPV
                     TVCLPKNASSQRKRILRHYGVEIIETDPLLSTDGAQRIARELFQNYPGAYFYPDQYNN
                     DANWLAHYETTANEIWNQTEGGVTHFVTGLGTSGTFVGVVRRLRELRPDLAAYSVQPD
                     SPMHGLEGLKHMATAKQPGIYDASLANGMLEVPTEEAVAMTRRLAREEGLFVGPSSGA
                     NVFAAIKLAKELVGPAVIVTILCDTGERYLEDI"
     gene            1501..2718
                     /locus_tag="VFH91_08965"
     CDS             1501..2718
                     /locus_tag="VFH91_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET6863168.1"
                     /translation="MTDKENDLNINTQLVHAGERQSLDQVNPVATPIYSTATFTYGSM
                     EEIDQVFSGEKAGYIYTRYGNPTIAGLEEAVRTIEGGATACAYSSGMAALHAALLACD
                     LKPGSTVVASQDLYGATNALLTNIFGTFGVKSIFIDFGDLDLLRRTVKENRPQALVAE
                     TISNPLLKICDIEACAEIAHESSARFVVDNTFASPYLCQPLKYGADLVIHSATKYLSG
                     HADAMGGLVISRDEMDKLALISVMKLAGGVLGVWEAHEIMRGLKTLALRLERQCDNAR
                     KLSIELANNEFIGRVFYPNVDSDPATRVLRKPFLCALVSIELKEDTRAAAFRFMDSLK
                     LCVRSTSLGDVFTSVLHPATASHRDLPPARRKELGISDGLVRISVGIEDIDDVLADIK
                     QALRVASSRSDES"
     gene            2715..3152
                     /locus_tag="VFH91_08970"
     CDS             2715..3152
                     /locus_tag="VFH91_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET6863169.1"
                     /translation="MITLDAERLAEIRRHGVADYPYECCGLLLGRYEQEGRKVVLETY
                     PISNAREESAKRNRFLIRPEELLKGERYARSRNVEVVGFYHSHPDSPAVPSQYDLEHA
                     WPTYSYIIVKTTAGEAVDLFSWEQRSDRSCFDQEEISISSVAG"
     gene            3208..3468
                     /locus_tag="VFH91_08975"
                     /pseudo
     CDS             3208..3468
                     /locus_tag="VFH91_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
     gene            3511..4662
                     /gene="moeB"
                     /locus_tag="VFH91_08980"
     CDS             3511..4662
                     /gene="moeB"
                     /locus_tag="VFH91_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET6863170.1"
                     /translation="MPELSNEEIMRYSRHLIMPEVGMQGQRRLKAASVLMIGTGGLGA
                     PVGMYLAAAGVGRIGIVDFDVVEESNLQRQIIHGTKDLGRRKIESARDRLRDINPHIE
                     IETHEARLSSQNAMRLFKNYDIVVDGTDNFPTRYLVNDACVLSGKPNVYGSIFRFEGQ
                     ASVFWAERGACYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGAIQANETIKIILGAED
                     IMVNRLLLFDAWSMKFREFKLRKDPQCPVCGEDPTIRELIDYEEFCGVAPHQLPADDP
                     KLDEITATELSARRLRGEKLQVIDVREPHEFEIARIPGTKLIPLGHVVSRMNEIDQEV
                     DTVVHCKGGVRSAKAIEALKRAGFSGRLINLKGGITAWSEDVDPSVAKY"
     gene            complement(4818..>5499)
                     /locus_tag="VFH91_08985"
     CDS             complement(4818..>5499)
                     /locus_tag="VFH91_08985"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6863171.1"
                     /translation="IFIPRESANTKQPEATTAWFGVDRNELIRLGVTPGLSVTADKHA
                     TRLGPNRFTARALDDRAGSTALILALRKLNPSTLRRRVIFAWSVREETGLEGAIALAR
                     RYGPTVKRVFSIDTFVSSDSPIESTRFAFSPLGKGAVIRGLDNSSVTPAVEIDRLLRI
                     ARAQRIPLLVGATNGGTDGSELVRYGAINAGLSWPGRYSHSPVEVLDLRDLEALVRVV
                     AALATAPP"
BASE COUNT         1340 a         1320 c         1500 g         1339 t
ORIGIN      
        1 ctatttgagt ccacatatgt agcgtcgcgc gggtgccttc ccattcctca agtggaagtg
       61 cgggccaggt ttcctgttta ttggctggtg ctgacattag gtctaccttt cttgctgtaa
      121 cggtgtgaca actataagtg ggttcgtccg gatcgcaaac cgtcaccacc gatcttgcct
      181 aatttgtagg ggagtccctc cgtgggcgcc ctctgtttca taaaagcttc gtaggcaaag
      241 gggggcgcca cggagggacg cccctacaaa tcagaagcgg cgttagcctt gacaggagaa
      301 ttggccaacc ctatagtttt ttcatcaact ttacgggttg cttcccaatg atgaaccact
      361 gcttctgttg tacttactgc tcatggtgct gttattgcag cgcgaccgat gggccgtgcg
      421 cagtgggagg aagcaaatga gctaacaact actcgcaatt attaaggtca taccggtcgc
      481 gccatcaaaa gatgggcgac cggtatgttt ttttgaaagt aatctacgtt acgcgtttcg
      541 cgtggaaaag aagcagttat gacactggct gttgagcatt tcgaaaccag actcacacct
      601 ggctcggttg aggccaatat cggcaacacc cctttgatcc gcctaaggct cgcagcgagt
      661 gatttgccag atgacgtcgg tatctttgca aaagccgagc agtttaatcc gggcggctcg
      721 attaaggacc gagcggcgct gtccatgatt ttggatggcg agcgaactgg aagactccgt
      781 ccgggaatga acatcgtcga tgcgaccagc ggaaacaccg gaatcgctta cgcaatgatt
      841 ggggcttccc gaggttatcc ggtcacggtc tgcttaccta agaacgcgag ttcgcaacga
      901 aaacggattc tccgacacta tggggtagaa attattgaga ctgacccgtt actgtccacg
      961 gatggggcac aacggatcgc gcgcgaactt tttcaaaact atccaggagc ctatttctat
     1021 cctgatcagt acaacaatga cgccaactgg ttagctcatt acgagaccac cgcgaacgag
     1081 atttggaacc agactgaagg gggagtaact catttcgtta caggcctcgg cacttccgga
     1141 acttttgttg gagtcgttcg ccgactacga gaactaaggc cagatctagc tgcatactcc
     1201 gttcagcctg attcgccaat gcatggtctc gaaggactca agcatatggc gacggctaaa
     1261 cagccgggaa tctatgacgc gtctctcgcg aatggtatgc tggaagtacc aacagaagag
     1321 gcagtggcaa tgacacgacg actggcgcgt gaagaaggac tattcgttgg accaagttcc
     1381 ggagcaaatg tgtttgcggc aattaaactt gcaaaggaac tggttgggcc agcggtgata
     1441 gtaactattc tttgtgacac aggagagcgt taccttgaag atatctgatt ggattccttc
     1501 atgactgata aagaaaacga cctcaacatc aacactcagc ttgttcacgc cggcgaacgc
     1561 caatccctgg accaggtcaa cccggttgcg actcccatct attcaacagc aactttcact
     1621 tatggctcga tggaagagat cgaccaggtt ttttcaggtg aaaaagccgg ttacatatac
     1681 actcgttacg gcaatccaac gatcgcgggt cttgaagagg cagtacgcac aatcgaaggt
     1741 ggtgcgactg cctgtgcgta ctccagcggc atggcagcac tccatgcggc cttacttgca
     1801 tgcgacttga aaccgggttc aaccgtcgtg gcctcgcagg atctttacgg tgctacgaac
     1861 gccttgctga cgaatatttt tggtacgttt ggcgtcaaga gtatctttat tgattttggc
     1921 gatctggacc tgttgaggcg cactgttaaa gagaataggc cccaggcact ggtcgccgaa
     1981 acgatctcta atcctttatt gaagatctgc gatatcgaag cgtgcgccga aattgcacat
     2041 gagtcgagtg cacgcttcgt tgtcgacaac accttcgcat ctccttacct ttgccagcca
     2101 ttaaagtacg gagctgatct tgtcatccac agtgccacga agtatttgag cggtcacgct
     2161 gatgcgatgg gtggtctggt gatttcccga gatgaaatgg acaagctggc tctcataagt
     2221 gttatgaagc ttgcaggtgg agtgctgggt gtgtgggaag cgcatgagat catgcggggc
     2281 ttaaaaactt tggccttgag acttgagcga cagtgcgata acgcccgcaa gctatccatc
     2341 gagctagcca ataatgagtt catcggtcga gtgttttatc cgaatgtcga ctcggatccg
     2401 gcgacacgag ttctgcgaaa accgtttttg tgcgcactgg tatcgataga actaaaggaa
     2461 gatactcgcg ctgccgcatt caggtttatg gattcactca agctgtgtgt tcgttccacc
     2521 agcctgggcg acgtatttac aagtgtgttg catcctgcga ctgcctccca ccgggatttg
     2581 ccgcccgccc gtagaaaaga gctgggtatt tcggatggac tggtgcggat ttccgtaggc
     2641 atagaagaca ttgatgacgt gttagctgac atcaaacaag cgttacgagt tgcatcgagc
     2701 cggagtgatg aatcgtgata acactggatg cggaacgttt agctgaaatt cgccgtcacg
     2761 gagtggcgga ctatccttat gaatgttgtg gcttgttgct gggccgttac gagcaggaag
     2821 gtcgaaaggt cgtactggag acttacccga tctcaaatgc aagggaagag agtgctaaga
     2881 gaaatcgctt tctgatcagg cctgaagagc tgttgaaggg cgagcgttac gccaggtcac
     2941 gaaacgttga agtcgtaggc ttttatcact cgcatccgga ttcgccggcc gtcccctctc
     3001 agtatgatct ggaacacgcc tggccgacgt actcttacat catagttaag acaacggccg
     3061 gtgaggcagt ggaccttttt tcctgggagc agcgttcgga tcgatcctgt tttgaccagg
     3121 aagaaatctc gatttcgtct gtcgcgggtt gaacgttaga agaacaagtg agtcaataga
     3181 ctttgggata cgcagaaagg aataataatg gctataacta tttcgattcc cgcaccgttg
     3241 cgccaattcg caggtgggca atctcaattt gttgtagaag caaagacggc gggtgaggct
     3301 ttagaccttt taacttcaac gcaccaggca ttgcgccgcc acctgtacaa cgatcaaaac
     3361 acgttgcgca attttgtgaa cgtctatcta aacgacgaag atattcgcca cgcctcagga
     3421 ccggagacac cagttaaaga tggcgacaat atcatgattg ttccgtcaat agctggcgga
     3481 gctgtcatcg agtcagaggt tggtgaagca ctgcccgaac tatcaaacga agagatcatg
     3541 agatatagcc ggcatctgat catgccggag gttgggatgc aaggtcagcg gcgtttgaaa
     3601 gctgccagcg tgctaatgat cgggaccgga gggctcggag ctccggttgg aatgtatctg
     3661 gctgcggctg gtgtggggcg gattggcatt gtcgatttcg atgttgtgga agaatccaat
     3721 ctccagcgac agatcattca tggcacgaaa gacctggggc gtcgaaagat cgagtcagcc
     3781 agagatcggc tgcgcgacat caatcctcat attgaaatcg aaacgcacga ggcaagatta
     3841 tcgagccaga atgcgatgcg tctttttaag aactacgaca tcgtggttga tggtactgat
     3901 aactttccca ctcgttatct ggtcaatgat gcgtgcgtct taagtggcaa gcccaacgtt
     3961 tacggctcca ttttccgttt cgaggggcaa gccagtgtct tctgggccga gcgcggagcc
     4021 tgctatcgct gtttgtatcc ggaacctccg ccaccgggcc ttgtgccgtc gtgtgctgaa
     4081 ggaggagtgc tgggtgttct gcccggcatt gtgggcgcca tccaggctaa cgagacgata
     4141 aagatcattc ttggcgcaga agacatcatg gtcaatcgac tgcttttgtt cgatgcctgg
     4201 tccatgaagt ttcgcgagtt taagctccgc aaggatccac aatgtccagt ttgcggagaa
     4261 gacccaacta ttcgagagct gatcgattat gaggagtttt gtggtgttgc tccgcatcag
     4321 ttgccggcag acgatccaaa acttgatgag atcaccgcga ctgagttgag tgccagacga
     4381 cttcgaggcg agaaactcca ggtcatagac gtacgtgagc cacacgagtt tgagattgcc
     4441 agaattcccg gaacgaaact gattccactc ggtcacgtag taagccggat gaatgagatc
     4501 gatcaagagg tcgatacggt cgtccattgc aaaggcggtg tgcgtagtgc caaagctatt
     4561 gaagctttaa aacgagcagg gtttagtggc cggttgataa atttgaaagg tggaattaca
     4621 gcctggtccg aggatgtaga tccttcagtt gcgaagtact aagtgagcga aggtgggccc
     4681 aaagaaatca agtatcaatt atcatttgag atttgatact tgttagtttc cgatggctct
     4741 ccagttcgcg cttgccgatt gagcgaagtg tttaaaacgc agggcagact tatcgaatct
     4801 caaatgataa ttgaaactta gggcggcgcg gtggccaacg cagcaacgac ccgaaccagg
     4861 gcctcgaggt cccttaaatc aagcacctct accggtgagt gactatatct tcctggccag
     4921 gacagtccag cattaattgc tccgtacctt accagttcag atccatcggt acccccgttt
     4981 gtcgctccca ccagcagtgg aatcctttgc gctcttgcaa ttcgcaggag acgatcaatc
     5041 tcaaccgctg gtgtgacact cgaattgtcg agcccacgta ttacagcgcc tttacccagt
     5101 ggagagaagg cgaatcgagt tgactcgatt ggtgagtcgg acgaaacaaa tgtgtcgatg
     5161 ctgaatacac gcttgacggt tggtccgtaa cgtcttgcca gtgcaatggc tccttccagt
     5221 cccgtttcct cccggacgga ccaggcgaag atgactcgcc tacgcagtgt tgacgggttt
     5281 agtttgcgca aagcaagaat gagtgccgtt gaaccagccc gatcgtcaag tgccctggct
     5341 gtaaaacgat tcggaccaag ccgcgtcgcg tgtttgtctg cagtgactga tagaccaggt
     5401 gtaactccca aacgaattaa ctcatttcgg tcaacgccga accatgccgt cgtggcttcc
     5461 ggttgttttg tattcgctga ttcccttgga atgaaaatg
//
LOCUS       DASSFP010000400         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_860009, whole genome shotgun sequence.
ACCESSION   DASSFP010000400 DASSFP010000000
VERSION     DASSFP010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860009"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..1709
                     /gene="serS"
                     /locus_tag="VFH91_08990"
     CDS             426..1709
                     /gene="serS"
                     /locus_tag="VFH91_08990"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6863172.1"
                     /translation="MLDLNYVRENLDTVRAALKARKGDVQALDDFAEADAQRRKVIAE
                     SDQLNAQRNASSREIGALMKEGKNEEAEKRRAEVGKLKEQIASLDHQRTETEAQMQQL
                     LSTLPNIPHSSVPVGEDESDNVEVRRWGTAPSFSFEAKDHVDLGEALGILDLERATKI
                     AASRFAILNGAGARLERALINFMLDVHTREHGYLETLPPFIVNRSALFGTGQLPKFEP
                     DLFKLEDDRELYLVPTAEVPVTNYYREEILDASDLPKRWTAYTPCFRSEAGSYGRDTR
                     GMIRQHQFEKVELVKYALPENSYAELEALTHDAEVILQKLGLHYRVVTLSTADLGFGS
                     AKTYDLEVWLPSQNTFREISSCSNYEAFQARRAQIRFRRAGGSKPEFVHTLNGSGLAI
                     GRTWIAIVENYQQEDGSILIPEVLKPYVGFDRIGP"
     gene            complement(1852..>3611)
                     /gene="sppA"
                     /locus_tag="VFH91_08995"
     CDS             complement(1852..>3611)
                     /gene="sppA"
                     /locus_tag="VFH91_08995"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET6863173.1"
                     /translation="LIITGILGAFLLLVVLCGAALWIALRGGEPSIRDNSVLALRVSG
                     SLPDYSPDDPLKRYFGGPSQSLSGLVMQFKKAKVDKRIKAVLLDIDVSGVGWGKSEEL
                     RDAITDFRSSGKPVYAYLEFGLNKEYYIATACDKIIVPPPGELFINGLAADVMFFRGS
                     LDKLGIYPDIYQIGKYKSAGDMFTQKEMTDAHREYINSLLDDLYNRYVDTIAKSRHKT
                     TDEVKALIDNAPYNAAKAKEAGLIDESLYRDEVEKQMKSMLGYKDSDPFVTVKGEYRD
                     VSPESLGLNEGERIAVVYASGDIGSGSSQNSPSGDQSIGSDTLAKAMNDAAADKTIKA
                     IVLRVDSPGGSGLASDIIWHAVEAANQKKPVVVSMSDVAASGGYYISASAAKIVAQPS
                     TITGSIGVVAGKPVMRGFYDWLGISNEYVLRGKTAGMFRETEKFSDDERAKFEDWIKT
                     TYYQDFIPKVAKGRKKDAQYVDSVGQGRVWTGFQAKERGLVDEFGGLDRAIDVAKELA
                     KIPADKGVQRVIFPYPTTFLQQLLSGNENSNTKVQQQEAVLAALPEDARRAFRYMAMM
                     ERMKNGDTMLIMPFELRVR"
BASE COUNT          917 a          915 c          866 g          913 t
ORIGIN      
        1 ggagtatgac gagccaccat cgtgaacgag tatgccgtat tccttgaact cagcggagta
       61 atagactgtg tggtccgggc aatcaaacgg gtctgaatgt tcagagcatt tgcgattcac
      121 ttctgtttcc atcgttccgc aacaatgatg tttcgctgtc aatgttccgt ccgtccgttc
      181 ctggtttagc tgagccaggc ggtctctaat aacatatacg gtcggaggag aacaaatgga
      241 gtcgtgcttt gtgctttgca cttcgttctt tgtgaccctt gtgttttgtg cctgggtctg
      301 caaaacaaag tgcaaagctc aaagtacaaa gctcaaagca caatgactgg gcgcgctggt
      361 tgacatgcct actccagcag ttgcaaagtc tgttaacttt tgcggcggga gattacagaa
      421 tctaaatgct cgatcttaat tacgttagag aaaatttgga tacagtgcgc gctgcactaa
      481 aggcgcgaaa aggtgacgtg caggctctcg atgattttgc tgaggctgat gctcaaagac
      541 gtaaagtgat tgcagagtcg gaccaactca atgctcagcg aaacgcgtcc agtcgggaaa
      601 tcggcgcgtt gatgaaggaa ggtaagaacg aagaggccga aaaacgacgt gcggaggttg
      661 gaaagcttaa ggaacaaatt gcctcgctgg accatcaacg aacagaaacc gaagcgcaga
      721 tgcagcaact gctctcaact ttacccaata tccctcactc aagtgtgccg gttggcgaag
      781 atgaatctga caatgtcgag gttcggcgtt ggggtactgc gccttcgttt agtttcgagg
      841 ctaaggatca tgtggatctt ggagaagcat taggaattct cgacctcgaa cgagcgacta
      901 agattgccgc ctctcggttc gcaattctga atggagcggg cgcacgactc gagcgagccc
      961 tgataaattt catgctcgac gtacatactc gagagcacgg ctaccttgag acgttgccac
     1021 cgttcattgt taatcggtct gcactgttcg gcaccggcca gttgcccaag ttcgaacccg
     1081 atctatttaa gctcgaggat gatcgtgaac tttaccttgt gccaactgca gaggttccag
     1141 ttactaacta ttatcgcgaa gaaattctcg acgcctctga cttaccaaag cgctggaccg
     1201 cctacactcc atgctttcgg tctgaagctg gaagttacgg acgtgacaca cgcggaatga
     1261 ttcgccagca ccagttcgaa aaagttgagt tggtaaagta tgcgttgccg gagaattctt
     1321 atgctgagct cgaggcacta acgcacgatg cggaagtcat tctccagaag cttggcttgc
     1381 actatcgagt tgtgactctt tcaactgcag atctgggatt tggatctgcc aaaacctacg
     1441 accttgaagt ttggttgcct tctcagaaca cgttccgtga gatctcgtcc tgttcaaact
     1501 acgaggcatt tcaagctcgc cgtgcacaga ttagatttcg acgcgcaggt ggttccaagc
     1561 ctgaattcgt tcacactcta aacggatcgg gcctggcaat tggaagaacg tggatagcaa
     1621 ttgtcgagaa ttatcagcag gaagacgggt cgatcctgat tccggaagtg cttaagcctt
     1681 acgtgggctt tgacaggatt ggtccttagc tacacctaat ctgaaccttc tctgtgcaac
     1741 ctttgtgtcc tctgtggctc tgtggtgagc aaagtcctaa gaaaaccatg gacacagagg
     1801 cacagaggac acaaaggctg cacagagtaa gtcaagttac agccgaggcc gttatctaac
     1861 tcgcagctca aaaggcatta tcagcatcgt atccccgttc ttcatgcgtt ccatcattgc
     1921 catgtaacgg aacgcccggc gcgcatcttc cgggagtgcg gcaagtacag cctcctgctg
     1981 ttgtactttc gtattcgagt tctcgttgcc acttaacagt tgctgcagga acgtagtcgg
     2041 ataaggaaaa attactcgct gaactccttt atcagcaggg atcttcgcga gttcttttgc
     2101 aacatcgatt gcgcgatcaa gtccgccgaa ctcatcaacc agccctcgtt ccttagcttg
     2161 aaatccagtc cagactcgac cttgaccgac ggaatctacg tattgagcat ccttctttcg
     2221 tcccttcgcc accttgggaa taaagtcctg gtaataagtc gtcttaatcc aatcttcaaa
     2281 ctttgctcgt tcatcatccg agaacttttc agtctctctg aacatgccgg cagttttgcc
     2341 gcgcaagacg tattcattcg agatgccgag ccaatcataa aaaccacgca tcaccggttt
     2401 gccggcaact acgccaatcg atcccgtgat agttgagggt tgggccacaa tctttgccgc
     2461 gctcgccgag atgtaataac cacctgaagc ggccacatca ctcatcgaaa cgacgactgg
     2521 tttcttttga ttggcagctt caacagcatg ccagatgatg tccgatgcca ggcccgaacc
     2581 accgggacta tcgacgcgca aaacaattgc tttgattgtt ttgtcggcag cagcgtcatt
     2641 catggctttc gccagggtgt cagatccgat tgattgatcg ccggatggac tgttttgaga
     2701 gctgcccgaa ccaatatcac cgcttgcgta aactacagca atacgctcac cctcatttaa
     2761 tcccagagac tctggcgaca catcgcgata ctcgccctta actgtgacaa atgggtcaga
     2821 gtccttatag cccaacatcg acttcatctg tttttcaact tcatcacgat acagcgattc
     2881 atcaatcaaa ccagcttctt tggctttcgc agcgttatag ggagcattat cgataagagc
     2941 tttaacttcg tctgtggtct tgtgacgcga cttagcaatc gtgtctacat atcgattgta
     3001 aaggtcatcc aaaagagagt tgatgtactc acgatgagca tcagtcatct ctttttgagt
     3061 aaacatgtcg ccagcgctct tatattttcc gatctgataa atgtccgggt agattcccag
     3121 cttgtctaac gaaccccgga agaacatcac atcagccgcc agtccattta tgaacagctc
     3181 tcccggagga ggcacaataa ttttgtcgca tgcggtagca atgtaatatt ccttgttaag
     3241 accaaactcg agataagcgt agacaggttt ccccgaggac cgaaagtcgg taatcgcatc
     3301 ccttagctct tcagattttc cccaaccaac ccctgagaca tcgatatcga gcagtactgc
     3361 tttgatgcgc ttgtccacct tagctttctt gaactgcatg acgaggccgg aaagagactg
     3421 actggggcct ccgaaatatc tctttaaagg atcgtcggga ctgtaatcgg gtagagagcc
     3481 agatactcga agagcaagca cgctgttatc tcgaatagac ggctctcctc ctctgagcgc
     3541 tatccaaagc gcggctccgc aaagcacaac cagaagcaga aacgcaccta agattccagt
     3601 tatgataagg a
//
LOCUS       DASSFP010000401         9687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_424515, whole genome shotgun sequence.
ACCESSION   DASSFP010000401 DASSFP010000000
VERSION     DASSFP010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424515"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..578
                     /locus_tag="VFH91_09000"
     CDS             162..578
                     /locus_tag="VFH91_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6863174.1"
                     /translation="MSKDETNRALSFADQLEQRLIDFAVRIIKLTDRLPKTAAGRHLA
                     GQILRSGTSPAPNYGEARGAESRADFVHKSRVVLKELNETWVWLQIIDRCEMVRPAQL
                     VDLIKENGELCKIFSTSLKTARQGKPNIKSQISNDN"
     gene            complement(613..1476)
                     /gene="lpxC"
                     /locus_tag="VFH91_09005"
     CDS             complement(613..1476)
                     /gene="lpxC"
                     /locus_tag="VFH91_09005"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET6863175.1"
                     /translation="MIKQTTLAKAVETHGIGLHTAVEVHLRLCPAPPDTGYVFRRTDL
                     GGFEIPATVESVAHCSYATTLMRTGVMLSTVEHLLAALRGLGVDNAFIEVDNLEIPIM
                     DGSAEDFADIIEQAGIVEQPLARRALLIREKVSFEQGNRRISVEPADTYRIECLIDFS
                     HPMIGVQSMSVELSNGSFSKDIAAARTFGFTDEIEALRRANLIRGGSLNNAIVLTTDS
                     MLNETQLRFRDEFVRHKILDIIGDLALLGMPILGKVVAERSGHIMHAGLMSKLLRVRS
                     AWDIVDMPNNN"
     gene            1615..1899
                     /locus_tag="VFH91_09010"
     CDS             1615..1899
                     /locus_tag="VFH91_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863176.1"
                     /translation="MAERHKLPGGRTSVTERHRGTEQLARRNKKLVYQVVASGQLRYS
                     RYLMITGGDPTQTRGTTRLNDLKNLNTARLLEYTQDQLNLSDALVLGVGV"
     gene            2427..3221
                     /locus_tag="VFH91_09015"
     CDS             2427..3221
                     /locus_tag="VFH91_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6863177.1"
                     /translation="MSHGTVQLELRDKVAVIVLNRPDRLNALTVAVAEDFIAAVNEAV
                     EKGARAVVLTGSGRAFCAGGDLREMQEIAGREGRVEAFFDEPLRLLNEVVLLIRSAAI
                     PFIAAVNGVASGGGCNLALACDVVIAARSAKFNQAFIKIGLSPDCGGTFFLPRLIGLR
                     RAAELMFTGDLVDAESAEQMGMINSVVEDGELMKHALAMAERLAAAPTAAIGQIKKLL
                     DASAASDLATQLDRERMSQIKMGQTKDFAEGVAAFLEKRPARFTGG"
     gene            3305..4627
                     /locus_tag="VFH91_09020"
     CDS             3305..4627
                     /locus_tag="VFH91_09020"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6863178.1"
                     /translation="MDSLTYAQLLQRNRSFRRLWLGQVISELGNWFNFIAALGLVRVV
                     SHAAPEATTIVLLTRLIPFTLFSPLAGAFVDRWSRRRVMIATDILRVFVALGFLFVHG
                     PEDLWVAYLCTGLLSFFGVFFEAAKNAAVPNITGERDLLAGNALMFSSRFFLMSIGAA
                     LGGWTSAHVGYQAAFVINALSFVASAYSVWLVPEDETRQPATANAALNAEKSRRSYWS
                     DIREGWSYIVTHRTVATILVTNIFWATGGGLMNLIADRLGALVFAGENGIGPDAAVAV
                     LYFGSGMGLFIGMLIARRVGGYFEFKERTVAFIGWSLLIQGLVFATAGFMPSIWLAGL
                     MILISRILLGAEFAVQETLLMKLVPDNLRGRVSTTDRATELLIWSFSTAVGGWSLYII
                     SPRTLTAISGVLSASAGLFWLILFAAQVVTMPVRLSQSRAEQETTVTS"
     gene            4850..5710
                     /locus_tag="VFH91_09025"
     CDS             4850..5710
                     /locus_tag="VFH91_09025"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6863179.1"
                     /translation="MQTINKRNMGINWSTTTFMIIFHVGALIGLYFFSWKLLLLAIVG
                     NWVAGSLGIGIGYHRLLTHKGFHTPKPVEYFLTFCGTLALEGGQINWVVTHRIHHAFT
                     EKAGDPHTPRDGRWWAHMGWILRGTAQQHEEKVMRRYAPDLMKDKFHVLLNRFYFVPL
                     IVSAIVLFALGGLPAVLWGVFVRVTFGLHGTWLVNSATHLWGKRRFETTDDSTNNWWV
                     ALITFGEGWHNNHHAFPTAARHGLKWYEIDINWWGIKALKLLGWAKGIKLVSKEQIAS
                     SHQGALRKAA"
     gene            5878..6786
                     /locus_tag="VFH91_09030"
     CDS             5878..6786
                     /locus_tag="VFH91_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6863180.1"
                     /translation="MRVSGRRKSIAFFVILGALLVALAIALNVGWIILNWREVALLVF
                     GIIFFLAIIFGLVINTTFLIREIRRNEQHDAFINAVTHELKTPIASIRLYLETLKTRD
                     VDEVQRNEFYNIMLADSDRLLQTVEQVLRAGRTGHRRRRISNSVVSLSEIVRECLEIT
                     RVRYGLNESHLAFSESPDVNGVRVSGDVDELRAAFSNLLDNAVKYSVGEVSVSVSVSA
                     VDEKRVAVKVADKGIGIPSAQLKRIFKRFYRVPGRFMARVKGTGLGLFIVRSVVEKHG
                     GRVFAESEGLGHGSTFTVQLPRFVRQ"
     gene            6783..7517
                     /locus_tag="VFH91_09035"
     CDS             6783..7517
                     /locus_tag="VFH91_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6863181.1"
                     /translation="MSSILIVEDEKHLADGLRYNLQAEGYEVEIVGTAEDAMVLFENP
                     TSHYDVLVLDVMLPGMDGFTLASHLRSAGEFLPILMLTARGQAEDVLRGFEAGADDYL
                     PKPFELSVLIARIHALLRRREWLARDQDTALNVTARDLPPPKVFDFGGKRIDFDALEL
                     KSGVATIHLTIMEADLLRYLIEHDGKIVSRSSILEDVWGLREDTDTRAIDNFIVRLRK
                     YLEDEPSRPKHLLTVRGVGYKFVSKT"
     gene            complement(7514..8440)
                     /locus_tag="VFH91_09040"
     CDS             complement(7514..8440)
                     /locus_tag="VFH91_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863182.1"
                     /translation="MHFSNGWLAFELGILRRLKFGSVALPFTGEPELIVQLKRWNVRV
                     AANDPMLWSYTKARALVENYGEQLSEDDCNRLLEDTYVPKEKLDNSGLLKWFNEVDAW
                     WFDNLRFNAESLEPYKRALALTAGMMVGDYVLSFNEQTRSLRKPFSLSDTFRLMLASL
                     PPVFDNALRNRSTNQDVRSFIAERHHTDLLFLRLPAPVMRTSSARDPILTWREEWLRG
                     GEDFWNEMDRERGARLGSRVQSKQQYLGFVDDFLRTAAHVPAWAIAHTENGFISSEEL
                     VESVGKVRKVAAIYSKDFSDLLGVRASLIIAS"
     gene            complement(8584..9255)
                     /locus_tag="VFH91_09045"
     CDS             complement(8584..9255)
                     /locus_tag="VFH91_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6863183.1"
                     /translation="MSAGASIELIDQSAAEAFQQLAITTDRFEYYENSHALRIAIIAD
                     ELAQVFHLARHDRGSLRAAALLHDLGEVAMERKYIQREGPLTEEERVDLSRHPVIGER
                     EASRVGADRAAQLLVRWHHESWNGQGYPDALRREEIPLAARILRLADCYAALTDSRPF
                     RGALTAEDARRELVGLAGIDFDPSVVNAFLSLEGVDELNSFAKPPAELIEDSSNTKHG
                     WNLFS"
BASE COUNT         2342 a         2304 c         2575 g         2466 t
ORIGIN      
        1 cgcgtacacg acatagttcg cagcggcctt taaaagcgat ctatttgaga attgtgaatt
       61 gatagttatg aattgttaat gttgaaatgg gagtcggatc ctgattgact tctcggatcg
      121 ggacgtgatt tcaaacccta cctcacgaag ggacctcacc catgtctaag gatgaaacca
      181 atagagcact gtcttttgcc gatcaacttg aacagcggct tatagacttc gctgtacgaa
      241 taataaagct gacagatcgc ttaccgaaga cagccgcagg tagacatcta gcgggacaga
      301 tccttcggtc tggaacctca ccggctccaa attacggaga ggcccgagga gctgagagtc
      361 gagccgattt tgttcataag tcgcgagttg tattaaagga attgaatgaa acgtgggttt
      421 ggcttcagat tattgatcgc tgtgaaatgg ttaggccggc tcagctcgta gacctaatca
      481 aagaaaatgg cgagctctgt aagatctttt ccacttcgct caagactgct cgtcaaggaa
      541 aaccaaatat caaatctcaa atatcaaatg ataattgatc aatagtagtt acgttcatga
      601 gtatgccggg tctcaattat tgttgggcat gtcgacgata tcccaggcgg atctcacacg
      661 gagcaactta gacataagac cagcatgcat gatgtggccg ctgcgctctg ccacaacttt
      721 tccgagtatt ggcatgccga gcagtgcgag gtctccgatg atgtccagaa ttttgtgacg
      781 cacaaactcg tcacgaaatc tcagttgcgt ttcattaagc atactatccg tagtcagaac
      841 gattgcattg ttcagcgaac ctccgcggat caagtttgct cgtcgtagag cttcaatctc
      901 gtcggtaaag ccaaaggttc gagccgcagc aatatcctta ctaaaagaac cgttgctcaa
      961 ttcaactgac atgctttgca cgccaatcat tggatgggaa aaatcgatca gacattcaat
     1021 gcggtaggta tctgcaggtt ctacgctgat gcgtctgttg ccttgttcga atgagacttt
     1081 ttcacgtatc agtagagccc gacgcgccaa cggttgttcg acaattccag cttgttcaat
     1141 gatgtcggca aaatcttctg ctgacccatc catgattgga atctcgagat tatcaacttc
     1201 gatgaaggca ttatcgacac ctagtcctct gagcgcagcg agcaggtgtt cgacggttga
     1261 aagcattacg ccggtgcgca tcaatgtagt cgcgtaacta cagtgagcga cggattcaac
     1321 tgtggcggga atttcgaatc cgccgaggtc ggtgcgacgg aagacatagc cggtatctgg
     1381 tggcgccgga caaagtctca gatggacctc cacagcagta tgtagtccga taccgtgcgt
     1441 ttcaactgct ttagctaacg tagtttgttt gatcaccttg ggccagcaac attaaaactt
     1501 gaattcaaga actgttcgtt tagagcaacc ttcgtgccct cttgaaatgg cggaatatca
     1561 gaggcacggc ccagttgatt gttgtctcgc cgcaacagtt tcctgcgcac gatgatggct
     1621 gaacgccaca agcttcctgg tggtaggacc agcgtaaccg aaaggcacag aggcacagag
     1681 caactggcgc gacgaaataa gaagttggtc tatcaggttg tagcatcggg tcagcttcga
     1741 tactccaggt acttgatgat taccggaggc gacccgacgc aaacgagagg tacaacgcgc
     1801 ttgaacgacc taaaaaattt gaatacagct cgtttactgg agtacactca ggaccaacta
     1861 aacctatcgg acgctctggt tttgggcgtt ggtgtttaag aaaaggcgct ttcgcccaca
     1921 gcgagtggtt gaaagaaaca caacgtcagc gctccgacga acaaagaggg gaagcctcag
     1981 attccactct tgtggagaac caagatgcca gaccttgaaa tcacgtgttc tgagtgtaat
     2041 aactccttta cattttcgga gcgcgaacag gagtactaca gggaacgcaa ccttacttac
     2101 ccaaaacgct gcaagccatg ccgtgaagca cggcgagcca attttggcgg ctcaagagga
     2161 ccgggaggtg aacgtcaacg attcgagatt acctgcgacc aatgcggcaa gactgattct
     2221 gttccattca agccttctac agggcgacca gttttgtgca gcgattgttt cagctcgagt
     2281 cgtgcccagc aaagaagcta acgcctcagc aacgccatcc gaacggtagc cgagccacag
     2341 ctatcgaacg aatgaaactt aagacaacca ggccgcgttg tagattccaa cgcgattaag
     2401 ggtaaaaacc gctctgaatt agaccaatga gtcacggtac tgtacaactg gaactccgag
     2461 ataaggtggc agtaatagtt ctgaaccgcc cggatcgttt aaatgctttg acagttgccg
     2521 tggctgaaga ttttatcgca gctgttaacg aagcggttga aaagggcgcg cgtgctgttg
     2581 tgttaaccgg atcggggcga gccttctgtg ctggcggaga cttgcgcgaa atgcaggaaa
     2641 tcgcaggcag ggagggtcgg gttgaagcgt tttttgacga gccactacgt ctattgaatg
     2701 aagtggtttt gttgatccgt tctgcggcga ttccatttat tgccgcagtt aatggcgtgg
     2761 cgtccggtgg aggatgcaat ctcgcgttag cgtgtgatgt agttatcgcc gccaggagcg
     2821 caaaattcaa tcaggccttt atcaagatcg gattgtcgcc tgactgtggc ggcacattct
     2881 ttttgccgag actgattgga ctaaggcgcg cggccgaatt aatgtttact ggcgatttgg
     2941 ttgacgccga gagtgctgaa cagatgggca tgattaactc agtggtggaa gatggcgagc
     3001 tgatgaagca tgcattagca atggcagaac gattggcagc ggcacctact gctgcaatcg
     3061 gtcaaattaa gaagcttcta gacgcgagcg ccgcgtcaga ccttgcaacg caacttgatc
     3121 gagagcgcat gtcacagatc aagatgggtc aaacaaaaga ttttgctgaa ggcgttgcgg
     3181 cattcctgga aaaaagaccg gcgcgtttca caggaggata gaccaggttc aacccgaaag
     3241 ttgttgcgcc gcctggttct gataccggcg gccttttagt tttggtacac aggttagaac
     3301 aaagatggat tcgttaactt acgcacaact gcttcagcgt aatcgttcct tccggcggtt
     3361 atggttaggc caggtcatct ccgaacttgg gaactggttc aacttcatcg ccgctttggg
     3421 actggtccga gttgtgtcgc acgcagcacc agaggcgaca acaattgttt tgttgacgag
     3481 actcataccc ttcaccctgt tttctccgct cgcgggagcc tttgtcgatc gatggtcacg
     3541 ccggcgggtg atgattgcga cagacatact gcgcgtattc gttgcgttgg gttttttatt
     3601 tgttcacggg ccagaggatt tgtgggtggc atacctttgc acaggtttgc tttcgttttt
     3661 tggcgtgttc tttgaagccg ccaagaatgc ggccgttcca aatattaccg gcgagcgaga
     3721 tttgttggcc ggcaacgctc taatgttctc ttcacggttc tttctgatgt cgatcggtgc
     3781 agcgctgggt ggttggacct ccgctcatgt tggatatcaa gccgcttttg ttatcaacgc
     3841 gctctcgttt gtcgcttcag cttattcagt gtggctggta cccgaggatg agacgcgtca
     3901 gccagcgaca gctaatgccg cactcaatgc agagaagagt cgtcgcagtt actggtctga
     3961 tatacgtgaa ggatggtcct acatcgtcac gcaccgtact gttgcaacga ttctggtgac
     4021 caatattttc tgggcgactg gcggtggact gatgaacttg attgccgatc gcctcggcgc
     4081 acttgttttt gccggtgaga atggaattgg tcctgatgct gcagtagcag ttctatattt
     4141 tggaagtggc atggggttgt tcattgggat gttgattgct cgacgggtgg gcggttactt
     4201 cgaattcaag gaacggacgg ttgcattcat cggatggagt ttgcttattc aaggtctggt
     4261 gtttgcaaca gctggtttca tgccctcaat ctggctggcg ggcttgatga tcttgatttc
     4321 gagaattctc ctgggtgccg agttcgctgt gcaagagacg ttgttgatga agttggtgcc
     4381 agataattta cggggacgag tgtcgaccac tgaccgtgct acggagcttc tgatctggag
     4441 tttctcgacg gccgttggag gatggtcact ttacattatt tccccccgca cactgacagc
     4501 gatctctgga gtgctctccg caagtgccgg actcttctgg ctgattttat ttgcggctca
     4561 agtggtgact atgcccgtac gtctaagtca atctcgcgcc gaacaagaga ctacagtgac
     4621 ttcgtagaaa taccaccaac ggggagttgg tggttcgtta aagtccaacc tacaacgcaa
     4681 cgcaacacgc catcgagttg gaattccacc aactgccgtt ggtggaattg gaaggaggac
     4741 atcgcaaatt acagaagcca attgccattt acacaagtat tacagtgctc tcgtttcgcg
     4801 tttgttagga ttcagtgtgc gcagcaatat cgtcggggtt ggagaaagaa tgcaaactat
     4861 caacaagcgg aacatgggta ttaattggag taccacgacc ttcatgatca tctttcatgt
     4921 gggcgcactc attggactct atttctttag ctggaagttg ttactccttg cgatcgtggg
     4981 gaactgggtt gcgggaagtt tgggaatagg aatcggctat catcgcttgc ttacccacaa
     5041 gggttttcac actccaaaac cggttgagta tttcttaacg ttctgtggaa cgctcgcact
     5101 tgagggggga cagataaatt gggtcgtcac tcatagaatt catcatgcct tcacagagaa
     5161 agcgggcgat ccccacactc cacgggatgg tcgttggtgg gcgcacatgg gatggatatt
     5221 gagaggtact gcgcagcaac acgaagagaa ggtgatgcgc cggtatgctc ccgatttgat
     5281 gaaggacaaa tttcacgttc tgctaaatcg cttttatttc gtgcccctga tcgtttcagc
     5341 tatcgtgctt ttcgcgttgg gtggactgcc agcagtgctc tggggtgtgt tcgttcgagt
     5401 aacctttggg ctacacggaa cctggctcgt caactccgcc acccacttgt ggggcaagcg
     5461 ccggtttgaa actactgatg attcaactaa caattggtgg gtcgcgctga tcacttttgg
     5521 cgaaggctgg cacaacaatc accatgcctt tccgacggct gcacgtcatg gcctgaagtg
     5581 gtatgagatc gacatcaatt ggtggggaat caaggcgttg aaattgctgg gttgggccaa
     5641 agggattaag ctcgtcagca aagagcagat agcctcaagc caccaagggg ctctgcgaaa
     5701 agctgcttag tttgtcagtg gtcccgttgt cagtggtccg ttgtcggtcg ttagtagtca
     5761 gtagtccgtg gggtcagttg tcagtagtcc gggtgttcga caaatcgtta agcgtcagtt
     5821 gatactagtg gaacgttaac gactgacaac gaacaactga caacggacaa aatttcaatg
     5881 cgcgttagcg gtagacgcaa atccatagcg ttcttcgtaa tattgggtgc cttgctcgtc
     5941 gccctcgcaa tcgcgctcaa tgtcggttgg atcattctta actggcgtga agttgctctc
     6001 ctcgtattcg gaataatctt cttcctcgcg atcatcttcg gactcgtaat caacaccacc
     6061 ttcctgattc gtgagatcag gcgtaacgaa cagcatgacg ccttcataaa cgccgttacg
     6121 catgagctaa aaacaccaat tgcttccatt cgtctttatc tcgaaactct caagaccaga
     6181 gacgttgatg aggtgcagcg aaacgagttc tacaacatca tgctcgccga tagcgaccgt
     6241 ctattgcaaa ccgttgagca agtcttacgc gctggccgaa ccggacaccg ccggcgacgc
     6301 atctcgaatt cggttgttag tttgagcgaa atcgttcgcg aatgccttga gataactcgc
     6361 gtgcgttatg gcctgaatga atctcatctc gccttttctg agtcgccgga tgttaatgga
     6421 gtcagggtgt cgggtgatgt cgatgagctt cgtgccgcat tctcgaatct gctcgataat
     6481 gcagtgaagt actcagttgg agaagttagc gtctccgttt cggtctcggc tgtagacgag
     6541 aagcgagttg cggtcaaagt agccgataaa ggcatcggca ttcccagcgc tcagttgaaa
     6601 aggatcttca agagatttta ccgggtgcca ggtcgtttta tggcccgtgt taaaggaact
     6661 ggattgggtt tgtttatagt acgttctgtt gttgagaaac atggcggacg tgtcttcgct
     6721 gagagcgagg gcctcggcca tggaagtacc tttacggttc agttaccacg gtttgtacga
     6781 caatgagttc aatattgatc gttgaagacg agaagcacct ggctgacggc ctgcgttaca
     6841 acctgcaagc cgaaggttat gaagtggaga tagttgggac ggccgaagac gcaatggtct
     6901 tatttgagaa ccccacttcg cattacgacg tgttggttct cgacgtgatg ttgcctggta
     6961 tggatggttt tactctcgca tcgcatcttc gctcggccgg cgaattcctc ccgattctaa
     7021 tgctgactgc ccgaggacaa gccgaagacg tgttgcgcgg cttcgaggcc ggggctgatg
     7081 attacctgcc gaaacccttt gagttatctg tactgattgc caggatccat gcgctgctcc
     7141 gaaggcgtga atggctagcc cgggatcaag acaccgcctt gaatgttact gcacgggacc
     7201 tgccgccccc gaaggttttt gattttggcg gaaagcgtat tgactttgat gcgctggagt
     7261 tgaagtccgg tgtagcaact attcacctga cgattatgga ggccgatctg ttgcgctacc
     7321 tgatcgaaca cgacgggaag attgtgtcgc gcagctcaat actcgaagac gtgtggggat
     7381 tgcgcgaaga caccgataca cgggctatag ataatttcat cgtcaggctg cgcaagtatt
     7441 tagaggatga accgtcacga ccaaaacacc ttttgacggt aagaggcgtt ggttacaagt
     7501 ttgtatcaaa gacctaactg gcaatgatca gtgaggctcg tacgcccagc agatccgaaa
     7561 aatctttcga ataaattgcg gcgaccttac gtacttttcc cacagattca accagctctt
     7621 cactcgaaat gaatccgttc tccgtatgag cgatggccca ggcgggaacg tgtgcggccg
     7681 ttctcaggaa atcatcaaca aaaccgagat attgttgctt gctctgtaca cgtgaaccga
     7741 gccgtgctcc tcgttcgcgg tccatctcat tccaaaaatc ctcaccgcct ctaagccact
     7801 cttcgcgcca tgtaagaatc ggatcgcgag ctgaactcgt tctcataacc ggcgcgggca
     7861 gacgcaggaa gagcaagtca gtatggtgcc gttctgcgat aaaacttctt acatcctggt
     7921 tggtactgcg atttcgaagc gcgttgtcga ataccggcgg aagggaagca agcatcaagc
     7981 ggaaggtgtc tgagagagaa aatggttttc gcaagcttcg agtttgttcg ttgaaggaca
     8041 agacgtaatc accaaccatc attcccgcgg ttagcgccag cgctcgtttg taaggctcga
     8101 gcgactccgc gttaaagcgc aagttatcga accaccacgc gtccacctcg ttaaaccact
     8161 tcaggagtcc agagttgtcc agcttttctt tgggaacgta ggtgtcttcg agcagtcggt
     8221 tacagtcgtc ttcgctgagt tgttcgccgt agttttcgac aagagccctg gctttagtgt
     8281 acgaccagag cattgggtcg ttggcagcga cacggacgtt ccatcgcttt aattgaacta
     8341 tcagttcggg ttctcccgtg aaagggagcg caaccgaacc aaatttcagg cgacgaagaa
     8401 tgcctagctc gaaggctaac caaccgttac tgaagtgcat agggtgtggg ctttaagttg
     8461 tctgttttgc aggttgttgc tttgtacttt gtgccttgta ctttgccttt gacttgtgta
     8521 tttgccgctt tcagatcacg aatcacaaag tacaaagcac aaagcacaaa gggcaaatcc
     8581 gcgtcaagaa aataagttcc aaccatgctt ggtgttactt gaatcctcaa tcaactccgc
     8641 tggtggttta gcgaaagaat tcaactcgtc aacaccctca agtgaaagaa atgcgttaac
     8701 tacagatggg tcaaaatcta taccggcgag tccgaccaac tccctacgtg catcttcagc
     8761 agttagagcg ccacgaaaag gcctgctgtc tgtcaaagct gcataacaat ctgccagtcg
     8821 taggattcgg gctgcaagtg gaatctcttc tcgccttaaa gcatccggat agccctgacc
     8881 attccacgac tcgtgatgcc accgcactaa aagttgagcg gcacgatcgg ctccaactct
     8941 tgaggcctcg cgctcgccga tgacagggtg ccgagaaaga tcgactcgct cttcttcggt
     9001 caatggtccc tcgcgctgga tgtacttgcg ctccatcgcc acctcaccga ggtcatgcag
     9061 gagcgcagcg gcacggagcg agccgcgatc gtgacgagcc agatgaaaaa cctgtgccag
     9121 ctcgtcggca ataatggcaa ttctgagagc gtgggaattt tcgtaatact cgaatctatc
     9181 agtggtgata gcgagttgtt ggaaggcttc agccgcagac tggtcgatta attcgatact
     9241 tgcgccggca ctcatagtgt tcgtttacat gtcccacgca atttcatcac agaagttggc
     9301 cttgagcaaa tgcaggttgt cagttgtccg tggtcagttg tccgttgttg tagtccagat
     9361 acccgttcga caaatgttag gtgtatacaa tcccggaatt cagagcactc cggaggagtg
     9421 agatgtttat agccggtgga ctaaaagaga tcctcgcact ccgtgaggag tgccatgttc
     9481 aacaaaccgt ccgaacgaat aaaagttttc ccggcgcatc aagtccgctg agatcacaat
     9541 gtttagagca accagactag ataaacatgg cactcctaaa ggagtaccta ataattcaat
     9601 cggtccgttg ctataaacat ctcactcctc cggagtgctc tgaattcccg gattgtatac
     9661 acctaacttt tgtcgaacag gtattag
//
LOCUS       DASSFP010000402         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1609007, whole genome shotgun sequence.
ACCESSION   DASSFP010000402 DASSFP010000000
VERSION     DASSFP010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609007"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..529)
                     /locus_tag="VFH91_09050"
     CDS             complement(167..529)
                     /locus_tag="VFH91_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863184.1"
                     /translation="MSVNSKSDSFSACLPDEIKPEMLVTLEPNPETTPANNAKPKPVT
                     VRQRLIQLKAHCKDGKLLDGQGKEIRFVQLIGCWGNPPEDYQEQLNQQQAEIKRLKEK
                     YIVVQISCAQDRDVKLIN"
     gene            817..>4274
                     /locus_tag="VFH91_09055"
     CDS             817..>4274
                     /locus_tag="VFH91_09055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6863185.1"
                     /translation="MSLELFHPAVARWFARTFPGATEPQLKAWPEIKQLKHTLIAAPT
                     GSGKTLAAFMSAIDDLVRLGVEGRLDNTTHVIYVSPLKALSNDIQRNLQFPLEGIQAE
                     LRAMGLPEINIRTLVRTGDTPASERTAMTKRPPHIVVTTPESLYILLTSDGGRRMLET
                     ARTLIVDEIHAVVGDKRGSHLALSVERLEQLVRQNVQKSREEALNQQLEFNQSSSLVR
                     IGLSATQKPIEEVARFLVGTANIKPSGEPDCSIIDSGHKRQLDIAIELPESPLQAVMS
                     GEVWEEVYERITQLIQQHKTTLIFVNTRRLAERVARHLGERIGEENIAAHHGSLSREH
                     RLTAEQRLKAGELSALVATASLELGIDIGDVNLVCQLGSTRSIASFLQRVGRSNHTVA
                     GFPKGRIFPLSRDELVETAAILDSVRRGELDHLEIPEQPLDILAQQVVAAVAPEEWTE
                     DDLFQTVRRAYPYRNLEREKFDSIVRMLAEGFSTKRGRRSTYLHHDAVNQRLRGRRGA
                     RLAAITSGGAIPDTADYAVVLEPSELVIGSVNEDFAIESLQGDIFQLGNTSWRVLRVE
                     QGKVRVEDAHGQPPSIPFWLGEAPARSQELSVSVSRLREEVGKRVEVRPVALEAVVDE
                     AESQSSKLVLEGSIKWLTEEVGVSRSGAEQIVEYLAGAQAVLGIMPSQENLVLERFFD
                     DSGSMQLVLHSPFGSRLNRAWGLALRKRFCRTFNFELQAAATEDAIVLSLGPTHSFPL
                     DDVFHYLKSQTVRQLLCQALLDAPMWNIRWRWNASRSLAVLRRRGGKKIPAQLQRMDA
                     EDLLTAIFPDQVACAENLAGGVREIPSHPLVDQTVKDCLEEAMDIDGLEKLLGSIERN
                     ERNLFARDVIEASPLAQEILNARPYAYLDDAPLEERRTRAVFQRRWLDPQTAGDLGKL
                     DQSAIDRVREEVWPEPRNADELHDALVELGFITAAEGVSWQSLFSALNVDRRAAVMKV
                     ATGDPNRPETDLWVAAERLPQLSAAYRIVDLEPPIAAPESIARTVYSEEEALAEIIRG
                     RLEGIGPITAPDLVATSGLSKLQIDTALLRLESEGFVIRGHFTPGTSETEWCARRLLS
                     RIHSYTLNRLRQEIEPVSGADFIRFLLAWQKVAPEHQMEGAESVKAVIEQLEGFEAPA
                     AAWEGEL"
BASE COUNT         1007 a         1077 c         1147 g         1043 t
ORIGIN      
        1 cagccaattt tctgacttta agaaacagtt tgaatgatgg tctttgacct ttgacctttg
       61 gcctttgctc tttggtcttt gctctttgtc agttgggggc gacttcaggt gagtcgagcc
      121 cttgatattg gagcgatcaa aaggccaaag gtcaaagacc ataaccctaa ttaatcaact
      181 tcacgtctcg gtcctgagcg cacgaaatct ggaccactat atatttttcc tttagccgct
      241 tgatttcggc ttgttgttga ttcaattgtt cctggtagtc ttccggcgga tttccccaac
      301 acccaatcaa ctgcacaaag cggatctcct ttccttgtcc atccaggagc tttccatcct
      361 tacaatgcgc ctttaactga ataagtctct gtcttacggt gacaggctta ggcttggcat
      421 tatttgctgg agtagtttcc gggttcggct cgagtgttac gagcatttcc ggcttaattt
      481 catccggcaa acacgcggag aaggaatcag atttggagtt cacagacatg cccagactca
      541 ggaaaattag actcaaagaa agtagtttca gcattgctaa ccaataatac tccagtgctc
      601 gtcagatgcg ccgagtccct gctcagtggc cgagacactc tgatcgtctg tggccgctcg
      661 agcgtcatca ttctccaaaa cgctgacagt agtcacattt cttaccgagg taaatgaaaa
      721 atggtaaatg acgaatgaaa aatgtcccgt ttcatggctg ggcgcgtgat tcagctatgc
      781 aaaaccagag tgtcgagttt ataatatgcc tcccttatga gtttggaatt atttcatcca
      841 gcagtcgcgc gctggtttgc gcgaaccttt cccggcgcca ccgagcccca gctcaaggcc
      901 tggccagaaa tcaaacagct caagcatacc ctgattgctg ctccaactgg ctcaggtaag
      961 actttggctg cattcatgtc tgccatcgat gaccttgtcc ggctgggagt agaaggaagg
     1021 ctcgacaaca ccactcacgt catctacgtt tcaccgctaa aggctctcag caacgacatt
     1081 cagcggaacc ttcaattccc acttgaaggg attcaagcag aactcagggc aatgggattg
     1141 cccgagataa atattcgtac cctggtgcga acaggcgaca ctcctgcttc tgaacgaacc
     1201 gcaatgacca aacggccgcc acacatcgtg gtgactacgc ctgaatcgct ttacatcctg
     1261 ttaacaagcg atggtggtcg ccggatgttg gagactgctc gtactctgat cgtggacgaa
     1321 atccatgccg tggtgggaga taaacgaggc tcgcatcttg ctctctcagt tgagcggctt
     1381 gagcaactcg ttcgacaaaa tgtccagaag tcgcgtgaag aagccttgaa ccagcaactg
     1441 gagttcaatc agtcttcttc tctagtacgc atcggtttat cagccacaca gaagccaatt
     1501 gaagaggtgg cgcgattcct ggtgggtacc gcaaatatca aaccgtcggg tgagcctgac
     1561 tgctccatca ttgatagcgg tcacaaacgt caattggaca ttgccatcga gctcccggag
     1621 tctcccttgc aggctgtgat gtcaggtgaa gtttgggaag aagtttatga acgcatcact
     1681 caactaatcc agcaacacaa aacaacgctg attttcgtca ataccaggcg actcgctgag
     1741 agagtggccc gacaccttgg cgagcgaatt ggcgaagaaa acattgccgc tcatcacggt
     1801 agcttatcga gggaacaccg gctcactgcc gaacagcgtc ttaaagcggg tgagttgagc
     1861 gccctggtcg caactgcctc acttgagttg ggaattgata tcggcgacgt gaatttagtc
     1921 tgccaactcg gatcgacccg gtccattgca agttttctcc aacgcgtcgg ccggtcgaat
     1981 cacacggtag cgggctttcc gaaaggacgt atttttccac tctcacgcga tgagttggtg
     2041 gaaacggccg ccattcttga ctcggtccgt cgcggagagt tggaccacct tgagatacct
     2101 gaacagccac tcgatattct ggcccagcaa gtagtcgcgg ccgtagctcc cgaagaatgg
     2161 actgaagatg atctcttcca gaccgtccgc cgggcttatc cttaccgcaa tctggaacgt
     2221 gaaaagtttg attcaatagt gaggatgctg gcggaaggtt tttcgaccaa gcgcggcaga
     2281 cggtcgactt atttgcacca cgacgcggtt aatcaacggc tacgaggaag gcgcggtgct
     2341 cgacttgctg cgattacctc aggaggtgca attcctgaca ccgccgatta tgcggtggtg
     2401 cttgagccaa gcgaacttgt catagggtca gttaacgaag actttgcaat tgagagtctg
     2461 caaggcgaca tctttcaact aggtaatacg tcctggcgtg tcttacgagt tgagcagggc
     2521 aaagtccgag ttgaggatgc tcatggccag ccaccatcaa ttcctttctg gcttggcgaa
     2581 gcgccggctc gctcgcagga gttatcagtc tcggtgtctc ggctgcgtga agaggtggga
     2641 aagcgagtag aggttagacc ggttgcgctt gaagctgttg ttgacgaggc tgagtcgcag
     2701 tcgtcaaagc ttgtgcttga aggatctatc aagtggctca ccgaagaggt tggtgtttcc
     2761 agatccggtg ctgaacagat cgtcgaatat ttggccggag ctcaagccgt gctgggaatt
     2821 atgccttctc aggaaaacct ggttttggag cgattctttg acgacagcgg cagcatgcag
     2881 ttggtcctgc actcaccgtt cggtagccgt ctcaatcgcg cctggggtct ggccttacga
     2941 aaaaggttct gccgtacatt caactttgaa ttgcaggcgg ccgcgactga ggatgcgatt
     3001 gttctttcgc ttggcccaac tcacagcttt ccgcttgatg atgtttttca ttatttgaaa
     3061 tcgcagacgg ttcgccagct tctatgccag gcattgctcg atgcgccgat gtggaacatc
     3121 cgttggcgtt ggaatgcttc gcgctcactg gcagtgctcc gtcgtcgtgg tggtaagaaa
     3181 attccagccc aactgcaaag aatggatgcc gaggacctgt tgacggcaat ctttcctgat
     3241 caggtcgcgt gtgcagaaaa tttagctggc ggtgttcgcg aaattccctc tcacccgctc
     3301 gttgaccaga ccgtgaaaga ctgtctggaa gaggcgatgg acatcgatgg gctcgagaaa
     3361 ttgctcggtt cgatcgaaag aaacgaaaga aacttgttcg cgcgagatgt cattgaagcg
     3421 tcacctctcg cgcaggaaat actaaacgcc cggccctacg cttatctcga cgatgctccg
     3481 ctcgaagagc gacgaacaag agctgtattt cagcgtcgct ggcttgatcc ccaaactgcc
     3541 ggcgacctgg gcaaacttga tcagtcggca atcgatcgag tgcgagaaga agtttggcct
     3601 gaaccgcgca acgctgatga attacatgac gctttagtgg agttaggatt tattaccgcg
     3661 gccgaaggag ttagctggca atccctgttt tctgcgttga atgtggatcg tcgtgctgcg
     3721 gtgatgaagg ttgcgaccgg ggatccaaat cgaccagaga ctgatctatg ggttgcagca
     3781 gagcgattgc ctcagcttag tgcagcttat cgcatagtcg atctcgaacc accgattgcg
     3841 gctccggaga gtattgcgcg gactgtttac tctgaagaag aagctctcgc cgaaatcatt
     3901 cgcggacgtt tggaaggaat tggaccaatc accgcgccgg atctggtggc cacttcagga
     3961 ctgagtaaac tacagatcga caccgcttta ctcaggctgg agtcggaggg attcgttatc
     4021 cgagggcact ttacacccgg aacttcagaa accgaatggt gtgcacgacg gcttctttca
     4081 cggattcaca gttacacgtt gaatcgtctt cgacaggaga tcgagccggt ctcgggagca
     4141 gacttcattc gcttcctgct ggcctggcaa aaggtagctc cagaacacca gatggaagga
     4201 gcagagagtg tcaaggcggt tattgagcag ttagaaggtt tcgaggcgcc ggcggcagcc
     4261 tgggaaggtg aact
//
LOCUS       DASSFP010000403         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1151001, whole genome shotgun sequence.
ACCESSION   DASSFP010000403 DASSFP010000000
VERSION     DASSFP010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151001"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..466
                     /locus_tag="VFH91_09060"
     CDS             98..466
                     /locus_tag="VFH91_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6863186.1"
                     /translation="MSMKLYVGNLAFQTNSSQLQELFASAGTVQSASVVEDRETGRSR
                     GFGFVEMSSAEEGAAAIAQFDGKEINGRALKVNEAKPRENRSGGGGGRGGFGGGGYRG
                     NGGGGGRSDFGGRNDRTPRW"
     gene            639..1853
                     /locus_tag="VFH91_09065"
     CDS             639..1853
                     /locus_tag="VFH91_09065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6863187.1"
                     /translation="MNFSDLNLDQELINRCTALAYTKPTPVQSKVIPLILEGADIIAC
                     AETGSGKTAAFLLPLIQKLGKRKSRGVRALVISPTRELVQQTLNAYNSLRFKHSPECI
                     SIIGGANVRQQRDALRSGAEVVIATPGRLMDHMERGLDFSRLEILVLDEADRMLDMGF
                     WPSVKMILEKLPKQRQTLLFSATMSSWIESTARAAMRSPRLIEVTQRGLPPTTVEQFA
                     YPVAAESKTALLLNLLERERDFDRVLVFTRTRRGAERLSHILKARDHSVNRIHADRTQ
                     PQREAALWDFKRGHTRVMVATDIAARGIDVDAVSHVINYDVPMAPEDYVHRIGRSGRA
                     GKKGRAITLVTPADELSIGGIERLTRQTMKRVLVPGFGGSTISDSPKLSGSAVRRSGG
                     SMVRSFRPRRGR"
     gene            1893..2246
                     /locus_tag="VFH91_09070"
     CDS             1893..2246
                     /locus_tag="VFH91_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863188.1"
                     /translation="MSERQTFSVGDRIEHTCIACGEDCGHIVSSLGKRGQITRITCPM
                     CGSRVAYKADSSAPRNLSDGKTSIPYDQSRAYRKGETVAHQMFGAGRVTAVIQPHKMD
                     VLFADRMRRLVCGNS"
     gene            complement(2540..3322)
                     /locus_tag="VFH91_09075"
     CDS             complement(2540..3322)
                     /locus_tag="VFH91_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863189.1"
                     /translation="MFWEPTEPVLFFAALASFLIVLEIGFRVGLRQRPRSDEVGKAHV
                     NALQAALLGLLALLLGFNFAMAASRFDARKNLIEEEVNAIETTYLRAQLLPSPQRQQA
                     DDLIRSYIDARLEFMRAGNDQAQLQKSYLDGSRIARQLWDMSSAMVAQDQSGGPKSMF
                     ISSLNDLINVNEKRRVALDNHVPELVIYLLFTVAVGAMGFIAYGYGLTGRRRHGTTAF
                     YAVLIALVLTTILDLDQPRSGLIRVSEDSMVHLKGSLDQNLP"
     gene            3767..>5188
                     /locus_tag="VFH91_09080"
     CDS             3767..>5188
                     /locus_tag="VFH91_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6863190.1"
                     /translation="MYKSFPILILLFVANSVGVMAQATKNKGQLTDNQNPTNSAPIAP
                     TARKEQSSTAQHLYSEGIKLTEEGQLSQAVEELQQAVRMDPEYAEAYAALGRAYFKMR
                     QWQNAVDCFRRASELKTKQRASQNAVRQRVAQPESKGAKIEMNPAPANLHSAVPPVKP
                     FKSGPSTSFTTPSPTNAQSKKSEKTNEVVVAAKSPSTVVGMSGHKDKLAEENIPKTQQ
                     GVAPTQTNTDVAAVKPLNSDKQEKTDPTPTSQQSAIPQPVKPFNSGSSTSFTTPSPNN
                     AQSKQSEKTNEVVVAAKSPSTVAGMSGHKDKQAEENIRKTQQGVAPTQTNTDVAAVKP
                     LNSDKQEKTDPTPTSQPSAIPPEAGTNLAAVKSPNLESRTAQPEPPADGPPALKTNVD
                     TAEPTKEAAGVKVAMSVTPSSTPLESKSVAPTPINTSSDEKLLTKIYRVGPNDVLDVQ
                     IDGTESPRSTLFTVTSAGFLEHPM"
BASE COUNT         1382 a         1357 c         1308 g         1141 t
ORIGIN      
        1 ttgagcattt cgaaccagac ccttcgatcg caaccgtttc gccgaagcca atccgattcg
       61 cagcttcttt ttgttcccat aggggaaagg taaatttatg tcaatgaaac tttatgttgg
      121 aaatctcgca tttcagacga atagctcgca attacaagag ctgttcgctt cagccggcac
      181 cgttcaatca gcgagtgtcg ttgaggaccg cgagaccgga cgttcgagag gttttggctt
      241 cgtcgaaatg tccagtgcag aagagggcgc agctgcaatc gcccagttcg atggaaaaga
      301 gatcaacggt cgcgcgttga aggtaaacga agccaaacca cgcgagaatc gaagcggcgg
      361 cggtggcggc cgtggcggat tcggtggtgg aggctatcgc ggcaacggcg gcggcggcgg
      421 aaggagcgac tttggaggtc gcaacgatcg aacacctcgc tggtaagccg tcctaaccgg
      481 cgacactttt ttgaaaagcg ccccggcgag caacaactta gtcggggcgc ttactttttg
      541 cggcgtgagc cagcaagctc tctttcgatt tcggaaatga ttatttgatc ctgagatatc
      601 taaggagtgc aaacagattg taacttggaa acgattaaat gaattttagt gacttaaacc
      661 tcgaccagga gttgattaac cggtgcacag cactcgcgta taccaaacca acacctgtac
      721 agtcgaaggt tatcccattg atacttgaag gcgccgacat tattgcgtgc gccgaaaccg
      781 gcagcggaaa aaccgcggcc ttccttcttc cgctcattca aaaacttggc aaacgaaaat
      841 cgcgaggtgt acgcgcactc gtcatttcgc ctacccgcga gttggtccaa cagaccttga
      901 acgcctataa cagtctgcgc ttcaagcaca gccctgagtg catttcaatc attggtggcg
      961 cgaacgtcag acaacagcgc gacgcattgc gaagtggcgc agaagttgtc atcgcaaccc
     1021 cgggccggct aatggaccac atggaacgtg gacttgattt ttcgcgcctc gagatcctcg
     1081 tgcttgatga agccgaccgc atgctcgaca tgggtttctg gccttccgtg aagatgattc
     1141 tggaaaagct accaaagcag cgccagacac ttttgttctc agcgacaatg tcgtcgtgga
     1201 tcgaaagtac cgcccgggca gcgatgagaa gtcctcgact tatcgaagta actcagcgag
     1261 gtcttccgcc aacaacggtt gaacagttcg cttacccggt agcggcagaa tctaaaacag
     1321 cgctactgtt gaatctactt gagcgcgaaa gagactttga tcgcgtgctg gtgttcacac
     1381 ggacaagacg cggcgccgaa cgactgtcac atatcttgaa ggcaagagat cacagcgtta
     1441 atcgcattca tgccgaccgc actcaaccac aacgagaggc ggcgctttgg gacttcaaac
     1501 gcggccatac tcgagtgatg gtagcaactg acattgctgc acgaggaatc gatgttgacg
     1561 ccgtttcgca cgtgatcaat tacgatgtgc cgatggcgcc tgaagactat gtccatcgaa
     1621 ttggtcgcag tggaagagcc ggcaagaaag gccgtgcaat aactctggtg actccagctg
     1681 atgaactgtc gattggagga atcgaaagac ttacacgaca gaccatgaag cgagttctgg
     1741 tgccagggtt cggcggctcg acaatatctg actctccaaa gctttcgggg agtgccgtac
     1801 gtcggtcagg gggaagcatg gttcgctcgt tccgtccacg ccgcggacgg taacttaaac
     1861 taactaccca agaggagaac aaagtaaatc atatgagtga aagacaaaca ttctctgtag
     1921 gggatcgaat tgaacacacg tgtatcgcct gtggtgaaga ctgcggacac atagttagct
     1981 cattaggaaa acgagggcag ataactcgca ttacttgccc gatgtgcggt tctcgagtcg
     2041 cctacaaggc cgactcgtct gccccacgaa atctttctga cggcaagaca agtattccgt
     2101 acgatcagag tcgagcgtat cgcaaaggcg aaacagtagc ccatcagatg tttggggccg
     2161 gccgggtcac cgccgtcatt caaccacaca aaatggacgt tctgttcgcc gatcgcatgc
     2221 gacgtctggt ttgtggcaat agttagcgct gcctgtcgat ccccaggctg gaatattctg
     2281 gccggtggaa gtgaatactc ttaaatcttc tttatgtcgg gaagaacctc atcttcccga
     2341 catttaaaat ccctctattc agtatcttcc ttttaaacct actcgctaat tcgtgcgcgt
     2401 cgacattttc gagcgcatac gtacttgcca cttgctgcga gggatatcca gcgcgtcgaa
     2461 ttgatcacta gcatccttaa gagactcgcc acccgctcgt cgagtcttca gataaagcgg
     2521 tactgaccct ccgctgactc tacggtagat tctgatcgag ggagcccttc aagtgcacca
     2581 tactatcctc gctcacgcga atcagaccac ttcgtggttg gtccagatcc aggatggtcg
     2641 taagtaccag cgcaatcaac acagcataga aagcagtagt accgtggcgg cggcggcctg
     2701 tcagaccata gccatacgca ataaatccca tagcgcccac cgccaccgtg aacaataaat
     2761 aaatgaccag ttccggaacg tggttatcca gtgccaccct gcgtttctcg tttacattta
     2821 tgaggtcgtt caacgaggag atgaacatag attttggtcc accgctttga tcctgggcca
     2881 ccatagctga gctcatgtcc cagagttgcc gggcaattcg agatccatcg agatatgatt
     2941 tctgcaattg cgcctgatcg ttacccgctc tcatgaactc gagtcgggcg tcaatgtagc
     3001 ttcgaattaa gtcgtcggcc tgttgtcgct ggggcgaggg caggagttga gcgcgtaggt
     3061 aggtggtttc gatcgcgttc acttcttcct cgatcagatt ctttctcgcg tcgaaccgcg
     3121 aggctgccat cgcaaagttg aagccgagca gtaacgccag aagaccgagc agtgcggcct
     3181 gaagtgcatt aacatgcgcc ttccctactt catcactcct tgggcgttga cgaaggccca
     3241 cccgaaatcc gatttcgagc actatgagga agctcgccaa tgcagcaaag aagagtacgg
     3301 gttcggtagg ctcccagaac atatgtgcaa gttttagtta cgagtctgtt atgtttttag
     3361 ctgcggcacc ataagttgaa acacaacgcg tcaacactta acgcggctgt cggactaaac
     3421 gccgcactga ttggattcgc cgccgcatag agcccaaact tggcctgctt atatttccat
     3481 tctcgcaagc gacgttgccg ttgacaagaa ccagaaatag ccgtcctaac ttggcttact
     3541 ttgtgccctc tgtgctcttt agtgagcaaa gtcctatgaa accatcacca cagagccaga
     3601 gtgatctcag aggccgcaca gagaatgttc aggttaggac tccactaagg ggtgcaacca
     3661 atggaattgg aagaataaaa atgctataaa aaagtccttt ctggcctcca tcgttagaat
     3721 ctgactgttt cagtcagtcg caaagcattt tgcaagggga tttctgatgt acaaatcctt
     3781 tcctatcctc atcctgctgt ttgtcgctaa ttcagtcggc gttatggccc aagcgacaaa
     3841 gaataaaggt caactgacag acaaccagaa tccgacgaat tctgctccta tagcaccgac
     3901 ggcccggaaa gagcaaagct cgaccgcaca gcacttgtac agtgaaggca taaaacttac
     3961 tgaggaggga cagctgtctc aggctgttga agaacttcaa caagcagtac gcatggatcc
     4021 ggaatacgca gaagcttatg ccgcgctcgg tcgtgcttac tttaagatgc gccaatggca
     4081 gaacgctgta gactgctttc gccgtgcttc ggaattgaaa accaaacaac gcgcatctca
     4141 aaatgcagtt cggcaacggg tagcgcaacc ggaatcgaaa ggagcgaaaa ttgagatgaa
     4201 tcccgctccg gcgaacttac acagcgctgt tcctccggtg aaaccgttca agtcaggacc
     4261 atcgacgagt tttacgactc cctctcctac caatgctcaa agtaagaagt ctgagaagac
     4321 caacgaagtc gttgttgctg cgaaatcacc gagtactgtt gttggaatga gtggacataa
     4381 agataagcta gctgaagaaa acattcctaa aacgcagcaa ggcgtggcgc caacccaaac
     4441 caacacggat gttgctgctg tcaaacccct gaattctgac aagcaagaaa aaaccgaccc
     4501 cactccaacg agtcagcaga gcgcaattcc acaaccggtg aaaccgttca attcaggatc
     4561 atcgacaagt tttacgactc cctctcctaa caatgctcaa agcaagcagt ctgagaagac
     4621 caacgaagtc gttgttgctg cgaaatcacc gagtactgtt gctggaatga gtggacataa
     4681 agataagcaa gctgaggaaa acattcgtaa aacgcagcaa ggcgtggcgc caacccaaac
     4741 caacacggat gttgctgctg tgaaacccct gaattctgac aagcaagaaa aaaccgaccc
     4801 cactccaacg agtcagccga gcgcaattcc accggaagcc ggcacaaatc tggctgccgt
     4861 gaaatcaccg aatctggaat cccgaacggc gcaacccgaa ccgcctgcgg atggtccacc
     4921 tgccttaaag actaacgtcg acacagcgga accaacgaag gaagctgcag gtgtcaaagt
     4981 tgcaatgagt gtcacgccgt catcgacgcc tctggaaagt aagagcgtcg ctccaactcc
     5041 aattaacaca tcgagcgatg agaaattgct cactaagatc tatcgtgtgg gtccaaatga
     5101 tgttttagat gtccaaatag acggtactga atcgccgaga tcgacgctct ttactgtcac
     5161 gtcagcaggc tttctggaac atccaatg
//
LOCUS       DASSFP010000404         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1610302, whole genome shotgun sequence.
ACCESSION   DASSFP010000404 DASSFP010000000
VERSION     DASSFP010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610302"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFH91_09085"
     CDS             complement(<1..705)
                     /locus_tag="VFH91_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863191.1"
                     /translation="MTKVEITVGSGAFRVYRSQQDQRDIHFESIQAETPEDQLQLKTE
                     VEQSLTVIRALFPNGSSRFDEYFQSLLSLAQCGLVGEAANPEVARRALSSWKNEVTAQ
                     EGGRIKNRYMIGLGIRALLLGAPPVGVSLIIFRYFTHSSELGGFLALWGGCMAGVWLS
                     FGARKAAFKFEDLHIPEADRLEPIVRLIFAGLLTTILGLAFSVSAVIVTIGSISTFEI
                     NTKFRVALLIGMLCGFS"
     gene            complement(702..905)
                     /locus_tag="VFH91_09090"
     CDS             complement(702..905)
                     /locus_tag="VFH91_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863192.1"
                     /translation="MLPPRLTHIKQSLEAKSSSKRTTSEDQLLDELRLLDDSAELRRF
                     LLTESRVMKMTSGPTAFCACCGR"
     gene            1085..1882
                     /locus_tag="VFH91_09095"
     CDS             1085..1882
                     /locus_tag="VFH91_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HET6863193.1"
                     /translation="MAKRADPPPPEPHEFRSPEEVDSAIGKLNRRVQELEKLDVRSAV
                     LNHTGAVKVIRSNVRETIREVFGSNSPEFDEHQYLDIWGGPMHMGMHHGEIVQGTERG
                     RVEVIAILKGLIGRLEEKRSDFVRGAVPAPSSYFDRLNLHPRIRDVARDRFIDGYHWD
                     AVFAGSKALLNFVKERSGLDIDGASLVRTAFSKNNPVLAFNELANQTDIDEQEGMMHL
                     FEGAVLAIRNPGGHAFPEGSEQRAIEYISLLSLLAYRVQEARLRRTP"
     gene            1946..>2374
                     /locus_tag="VFH91_09100"
     CDS             1946..>2374
                     /locus_tag="VFH91_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863194.1"
                     /translation="MEQRSVRSSKMTQGEIDRLVKDYIGTSSGYLNHFTYKIHETFYQ
                     RYCELDIDVLSYRQRGMTTLNAFTEILRTAPPDHQSRIIRGVFDMIPPPEDSPDKAVR
                     KKIAVYSALMQVASQLEPNRAIQEPNLPRGRDELGFYSARN"
BASE COUNT          615 a          598 c          607 g          554 t
ORIGIN      
        1 actgaatccg cagagcattc ctataagtag ggcaacgcga aatttggtgt ttatctcaaa
       61 tgtgctgatc gatccaatgg tcactatcac ggcgctcact gaaaaggcta aacctaagat
      121 ggtagtcagg agtccagcaa aaatcaggcg gacgattggc tcaagtctgt cagcctccgg
      181 aatgtgtaag tcctcaaatt tgaatgcagc cttgcgagct ccaaaagaaa gccagacgcc
      241 cgccatgcat cctccccaca acgccaaaaa cccccctagt tcggaactat gggtaaaata
      301 acgaaagata atcagtgaaa caccaactgg cggtgcgcca agtaaaagag ctcggatgcc
      361 gaggccaatc atgtagcggt tcttgattcg ccccccttct tgagcagtga cctcgttctt
      421 ccatgacgat aatgctcgtc gggctacttc aggatttgcc gcttcgccta cgagcccgca
      481 ctgcgcaagc gagagcaggg actgaaaata ttcgtcaaat cggctgctac cgttgggaaa
      541 aagagctcga ataactgtca gcgattgctc gacttcagtc ttcaactgca gttgatcctc
      601 aggagtctcc gcctgaatag attcaaagtg aatgtctcgt tggtcttgtt gactgcgata
      661 aacgcgaaag gcaccgcttc ccacggtaat ctcgactttc gtcatctgcc gcagcaggcg
      721 cagaatgcgg tcggtccgga cgtcattttc atgaccctac tttcggtcaa cagaaaccga
      781 cgaagttcgg cactatcgtc caacagtcgc agctcatcga gcaactgatc ctcgctggta
      841 gtccgtttac tggaagattt cgcttcgagt gactgcttga tgtgcgtcaa gcgcggtggc
      901 agcatgggca gctcctttcc attatggaac tctaaattat taaagaccgg tatactgatg
      961 atagctactt actgccggaa taaccaactg aaatccggtt tagtatacgc tttggttcgc
     1021 tacggcggtg tgcggacgtc ctgtcattga cttgcaaagt tacgatgctg agaataaggt
     1081 gcaaatggca aagcgagcag atcctccacc tcccgaaccg cacgagttta gatcacccga
     1141 agaagttgat tccgcgatag gtaaactcaa ccgtcgcgtc caggaacttg aaaaactcga
     1201 tgtcaggtcg gcagtcttaa atcacacagg cgctgtgaag gtaatccgga gcaacgttcg
     1261 cgaaacgata cgggaagtat tcggctctaa ctcgccggaa ttcgatgaac atcagtacct
     1321 agacatttgg ggcggtccaa tgcatatggg aatgcatcac ggcgagatcg ttcaaggtac
     1381 ggagcgcggc cgcgttgaag ttattgcgat tctaaagggg ctaattggcc ggctcgaaga
     1441 aaagcgttct gattttgtcc gtggggcggt ccctgcgcct tcttcatatt ttgatcgctt
     1501 gaacttacac ccacgaatac gtgacgtagc gcgtgatcgc ttcatagatg gataccactg
     1561 ggacgcagtt ttcgctggat cgaaagcact gttaaatttc gttaaggaac ggtccggtct
     1621 cgatattgac ggtgcatctt tggtgagaac agcattttcg aagaacaatc cggtcctggc
     1681 attcaatgag cttgccaacc agaccgacat cgatgagcag gaaggtatga tgcatttgtt
     1741 cgagggtgct gtgttagcga tccgcaatcc aggcgggcac gctttccccg agggctccga
     1801 acagcgggcg atcgagtaca tctcccttct gagccttttg gcatatcggg tgcaagaagc
     1861 caggctacga agaactccct gaccacatgt tctctttagc tcaaaaattg gttgccgttg
     1921 gcttagtgaa gttgcggaga gcttgatgga gcagcgatct gtgcgttctt cgaagatgac
     1981 tcagggagaa atcgatcggc ttgttaaaga ttacattgga acaagcagcg gatatctgaa
     2041 ccactttacg tacaagatcc acgagacttt ttaccaacga tactgcgagt tggacatcga
     2101 cgtcctatca tatcggcaac ggggcatgac cactctgaac gcatttacag agatactcag
     2161 aacggcgcca ccggaccatc aatcgcgaat catccgcgga gtcttcgaca tgattcctcc
     2221 cccggaagac tcaccggata aggcggttcg aaagaaaata gccgtctatt cggcgttaat
     2281 gcaggttgcg tctcagctag aaccaaaccg cgcaatccaa gaaccaaacc ttccgagagg
     2341 gcgcgacgaa ctcgggtttt atagtgcgag gaat
//
LOCUS       DASSFP010000405         9104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1311236, whole genome shotgun sequence.
ACCESSION   DASSFP010000405 DASSFP010000000
VERSION     DASSFP010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311236"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFH91_09105"
     CDS             complement(<1..698)
                     /locus_tag="VFH91_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863195.1"
                     /translation="MSTQMKAREFNDLDISSIRDQASQVVKLANTITEIAREVADGST
                     LQVGALGAAVTSTDELLTSLKETAERTSTIVISAEETSSAIVESSTSIEQVTGNAVSL
                     ASSINENSAAIEETAASIQSVKELSDQMTASAESVSGASTQLSSAIKGVVEDTEALTS
                     SINETAAAMEETTRTTQGLSQNADDLTVAAEQTSSAISEMGASIEEVSAMTQNLTTSV
                     EDVSASMEEMARSI"
     gene            complement(798..1322)
                     /locus_tag="VFH91_09110"
     CDS             complement(798..1322)
                     /locus_tag="VFH91_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6863196.1"
                     /translation="MENSLLTGMSTESNKFILFELMNTSYGVRSAFVRRLEMVENITP
                     VPNANPTVEGVVLSRGRIIPAVNLRTRFGFPKAPFDLRTRLVIVESEGRTVGLIVDSS
                     REFKTINETDIKLPPEGITNLNGEYLEGIATVDGRLILILELNEILKVGDSVSDESIN
                     LSGKDQTDQELRTP"
     gene            complement(1452..3401)
                     /locus_tag="VFH91_09115"
     CDS             complement(1452..3401)
                     /locus_tag="VFH91_09115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET6863197.1"
                     /translation="MADETSDNFFNEFLDDYFAESDEHLTIVRSRLLAIEQFVGRASV
                     DSAHLDELFRSFHTLKGISAMVGLTPAEQLAHKMESYLGALREGRLILLREGFEALIE
                     GTRTLEQVILAHRNNETSPDITKSADIIEALTSAPSQQDSPVEIKSTERASNKDRRTK
                     REWRFVFVPGPAHAAAGVNVNSVRARLQNIGEIIRSTPLIQEGDIAFEFIVLSDVDES
                     ELLDLGESGVTFSALAEVPYETESETSPETTETSAVPASLSAAPTSVRVDLMRLDQLM
                     LLVSDLVVSRARLETKLKTLRTSISANDWRGLQEVSHALGKQLRDLRNGVMRVRMVPI
                     GEIFERMRFVVRDLARETGKTVALDISGADTEIDKFLVERLMDPLLHIVRNAISHGLE
                     GEAERVAAGKPPEGHVRLRAQTAGEVVVIEISDDGRGIDVDEIAARNPDIQLFDAGGQ
                     VDLPQLLKIISTPGFSTRDQVDRASGRGVGMDVVHQSISELEGSVSLETTRGEGTFFR
                     ISLPLTLAITDALIASVGGQMFALQQSAVQEVVEIDPKSIKIMENNEIVLYRNRVLPL
                     VHLSNIFGYERKETGTLYAFVVGTGARATALVVDKVLGLREVVVRSLTDPLLDVKGIS
                     GATELGDGRPILILDALELLSGRAG"
     gene            complement(3409..3789)
                     /locus_tag="VFH91_09120"
     CDS             complement(3409..3789)
                     /locus_tag="VFH91_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6863198.1"
                     /translation="MTTEQENKILIVDDSRTIRRMIAKSLASIHKTTFEEAENGLEAI
                     ERLVLGPVSLMVLDLNMPEMHGIEVLKFVRSRPAYRSMPVVVLTTRGDEVSQADAMAA
                     GASLYLTKPFSPQELESKVRQLLT"
     gene            4578..6503
                     /locus_tag="VFH91_09125"
     CDS             4578..6503
                     /locus_tag="VFH91_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HET6863199.1"
                     /translation="MARVIDPTGNSTQKASSESNPELNSRKVMEDFRAIQAAAKASSD
                     GNLCAVAPAPELKERISKEIADIRANSKSAIASLARAAEPNRPGFNDGLILPPDLFPL
                     GTPLSAIRNAALDRAPLRGSVRVIVVLVDFSDKHMTQTQAHFRDLFFSTGVIPTGSVR
                     EYYTEVTHGLIDLQGDVVGPFRMPLSLAQYAHGAAGIGGASPNAQTMALDAVIAADPT
                     VNFTPFDNDGNGFVDAFIVVHAGAGGEQTGNPGDIWSHKWTLAGGAKSVDATKIFAYL
                     TVPEDSKIGVCAHELGHLLFGFPDLYDTDQTSEGIGNWCLMAAGSWGGGGDVPVHPSA
                     WCKANQGWVSVDNRTSNSTVTIPDVKDSNTVLRLWKDGAPGNEYFLVENRQKNRFDKS
                     LPQGGLLIWHVDDSVSTNTNESHYKVALMQADANRDLELDHNRGDGGDPYPGSSGNTS
                     FNKSSSPNSKSYSGVDTCVAVNAISASGAVMTANVAVKCKGVEKDQLKDIRDVFDKRR
                     IKEIREKRLEKPQIDKLYSYEKGFTDKLSDKFSDKLSDGKFTEGGGFPGGFGGGFAPP
                     DARLNQLEARVSALEAAAMQKPGEEAASPFIGSELRPDLSEGALAAEDDYSTLDEQMR
                     QGSGQAKRLFDSKLKEQ"
     gene            6505..7290
                     /locus_tag="VFH91_09130"
     CDS             6505..7290
                     /locus_tag="VFH91_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863200.1"
                     /translation="MLRFLILAHRGDATAMLVASHLLTRHRRESVRIVLADEFVSTAQ
                     LVHRVNGAKALTSLRLADGFSIKNDGIGIVFNRLRYVEIPQFARANMVDQEYAALELF
                     ACLISWMKSLTCPVFGAPSSQSLGGPTLSTLAWNVLAGKAGLPTSLVRFTSSLKYYSL
                     KGTAKYPIGNSVNGDVPAILSHQSGAPGIAVAVIGERVIGPVPHDLEAGCLRLAKLSG
                     FEVLVVYFSTEENNLMFGSVDAYPQLTDPQSINAVVNTLEERS"
     gene            7323..8243
                     /locus_tag="VFH91_09135"
     CDS             7323..8243
                     /locus_tag="VFH91_09135"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863201.1"
                     /translation="MALAIEAAQRLGLDQVVFNQRHAQFCDIAVDSHRGTLAGALRIS
                     DREWRLEDFSGVYTRLIDVHSLPENRPRRYTKPDDAAILKSSFLHQTLADWMEIAECR
                     VVNRAAAMSSNVSKPYQAQIVSKFFSVPATLVTNDPGAALEFIQLHRRVIYKSISSIR
                     SIVREWSAADEPSLYKIRSLPTQFQSYVEGTNIRVHVVGSRIFATEIISTAVDYRYAT
                     RDELEVEMVETELPLEIADKCVGLSMELQLPFCGIDLKRSPDGRYYCFEVNPSPAYSY
                     YQETTGQPIADALVEYLAGRTEVDHECSSC"
     gene            8224..8523
                     /locus_tag="VFH91_09140"
     CDS             8224..8523
                     /locus_tag="VFH91_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863202.1"
                     /translation="MNVQAVENWALIKGKVLSIRPHPELDRYAVAKIAVEEVTPVSGY
                     ANMFAWAVGKEVEFNIPQPKVDEVSLSEGDKISAKVRKGTPTASFVDADSVVKLG"
BASE COUNT         2206 a         2266 c         2322 g         2310 t
ORIGIN      
        1 tgaatagacc gcgccatctc ttccatagag gcagaaacat cctctactga tgttgtgagg
       61 ttttgcgtca ttgccgaaac ctcttcgatg gatgcaccca tctcgcttat agctgatgag
      121 gtttgctcgg cagcgacggt cagatcatcg gcattttggg aaagaccttg agttgttctg
      181 gtcgtttctt ccattgccgc ggcggtttcg ttgatcgacg aagtcagcgc ctcagtatcc
      241 tctacgaccc cttttatggc gctcgacagt tgtgtcgaag caccggaaac ggactcggca
      301 gaagccgtca tttggtccga caattctttg acactttgaa tggaagcggc agtctcttca
      361 atagctgcag aattttcgtt tattgatgag gccaaactga cagcatttcc ggtcacttgc
      421 tcgatcgacg tggaactctc gactattgcg gatgatgtct cttcggcgga aataacgatc
      481 gtcgatgttc gttcggctgt ctctttgagc gaggtgagga gttcgtcggt gctggtgact
      541 gcggcgccca acgctccgac ctgcaatgtc gatccatcag ctacttccct ggcgatttcg
      601 gtgatggtgt ttgcaagctt aactacttgt gaggcctgat cgcgtatgct cgaaatgtcc
      661 agatcattga attctcgagc tttcatttga gtcgacatta ctggctcctt aaaaagttgt
      721 cgctttccta tctatttagc ggaatggcat caacgccacc cgcactcatt gctatagagt
      781 cacctgactc atcaggacta cggcgttctt aattcctgat ccgtttggtc cttcccactg
      841 agattgattg attcgtcgga aacagaatct ccgactttta gaatttcatt gagctccaat
      901 atcaagatca atcggccatc gactgttgcg atgccttcca gatactcgcc gttaaggttt
      961 gttataccct caggaggcaa ttttatatcc gtctcattaa tagttttgaa ttctcgtgat
     1021 gaatcgacaa ttaagccaac tgttcgacct tcggattcaa cgataaccag gcgtgtgcgc
     1081 agatcaaacg gagctttagg aaagccgaaa cgagtgcgca agtttaccgc cggaataatg
     1141 cgcccacgag aaagaacgac gccttcaacg gttggattcg cattcgggac cggcgttatg
     1201 ttctcgacca tttcgagtcg acggacgaag gcgcttctta cgccataact cgtgttcatc
     1261 aattcgaaga ggatgaactt gtttgattcg gtcgacatac cggttaagag actattttcc
     1321 attatttcca ttctccattt atcattttcc atttttcatt tggcgttaga gggaggctcg
     1381 actatcaaaa cggaggctca aataagacaa aaatgaaaaa tggaaaatga caaatggaca
     1441 atgatccttt cctaccccgc ccttcctgaa agaagctcca gcgcatcaag aattaaaatc
     1501 gggcgaccat ctcccagttc tgtggcgcca gaaattcctt tcacatcgag caaggggtcc
     1561 gttaaagaac gaactaccac ctcgcgcaaa cctaaaactt tatcaactac cagggctgtt
     1621 gcgcgtgcac cggtccctac aacaaacgcg tacaaagttc cagtttcttt tcgttcgtaa
     1681 ccaaagatgt tcgagaggtg caccagagga agcacgcgat ttcgatacag tacgatctca
     1741 ttgttttcca taatcttgat cgatttcgga tcgatctcga ccacttcctg cacagcggat
     1801 tgttgcaggg caaacatttg gccaccgact gaggctatca atgcatcagt gatggcgagc
     1861 gttaacggca gagagatgcg aaaaaaagta ccctcacctc gggtcgtttc cagagaaacc
     1921 gatccttcaa gttctgagat cgactgatga accacgtcca tcccgacgcc acgtccactc
     1981 gcacggtcaa cctgatcgcg agttgagaat ccgggagtcg agattatctt tagtagctgc
     2041 ggtaaatcta cttgcccacc ggcgtcgaat aattgaatgt ccgggttgcg cgcggcaatc
     2101 tcatcgacat cgatacctct gccgtcgtca ctaatttcga tgactacaac ctcgccagcg
     2161 gtttgcgctc gtaagcgtac atgaccctca ggcggctttc cggctgcaac cctttctgcc
     2221 tctccttcaa ggccatgact gatcgcgttt cgtacgatgt gaagcagcgg atccattaac
     2281 cgttcgacaa gaaacttatc gatctcggtg tctgcgccgc tgatatccag cgcgacggtt
     2341 ttgccggttt ctcgagcgag atcgcggacc acgaatctca tgcgttcgaa tatttctccg
     2401 atagggacca ttcgaacccg catcactcca ttgcgcaggt ctcgcaattg ctttccgagt
     2461 gcgtggctta cttcctggag acctcgccaa tcgtttgccg agatcgaagt gcgcagcgtc
     2521 ttcagttttg tctcgagtct tgccctgctc acgaccagat cgctcaccag aagcatcaat
     2581 tggtcaagac gcattagatc gacgcgaacg gacgtaggag cagctgacaa cgatgccggt
     2641 acggctgatg tctcggttgt ttccggtgat gtttcggatt cggtttcgta cggtacttca
     2701 gcaagagcgc taaaagttac ccctgattct cccagatcta gcagctcaga ttcgtccaca
     2761 tcgctgagta caataaactc gaaagcaatg tcgccttcct gtatgagcgg agtactccga
     2821 atgatctccc caatattctg aagacgcgcc cgcaccgagt tcacgttgac tccagcagcc
     2881 gcgtgagcag gacccggaac gaagacaaat cgccattctc tttttgtgcg ccgatcttta
     2941 ttactagctc gctcggttga cttaatttct acaggcgagt cctgttgtga tggagcagat
     3001 gttaatgctt cgatgatgtc cgccgacttt gtaatgtcag gcgaagtttc attattccgg
     3061 tgcgccagaa tcacctgttc aagcgtacga gtgccttcga taagcgcttc aaacccttcc
     3121 cgaagcagga taagtcgtcc ttcccttaaa gctcccagat aactttccat cttatgggcc
     3181 agctgttctg ccggtgtgag ccccaccatt gctgaaatac ctttcaaggt atgaaaggag
     3241 cgaaagagtt catcaaggtg agcactatcg acggaggctc gaccgacaaa ctgttcgata
     3301 gcaaggagcc ggctcctgac gatcgttaag tgctcatcgc tctcagcgaa gtaatcgtcg
     3361 aggaattcgt taaagaagtt gtctgaggtc tcgtcagcca tcgctttcct aggtcaacaa
     3421 ttgtcggact ttgctttcaa gctcctgtgg ggagaaaggt ttggtcaaat agagacttgc
     3481 tccggcggcc atggcgtctg cctggctaac ttcgtcgcca cgagtcgtca aaaccaccac
     3541 cggcatcgat cggtaagccg gacgcgagcg cacgaacttc agaacctcaa tgccatgcat
     3601 ttcaggcata ttaaggtcca gcaccatcaa tgaaacaggc ccgagtacca gtcgttcaat
     3661 agcctccagc ccgttttcgg cctcttcgaa ggttgttttg tggatgcttg cgagcgactt
     3721 ggcaatcatc cgtctgattg tgcgggagtc gtcgacaatc aggatcttgt tttcctgttc
     3781 agttgtcaca attaggttct atggattggc gcgaatccag atctcggcgc ggtagcaata
     3841 tcggattcta actttaactg cggaggggtc ctcgggggta cggtaggaca ttagtataac
     3901 gcctgatatt tggcaagcat cgccacagac cagtattggt tcagtttgaa ttctttctca
     3961 gtctcacggt gacttggagc cggtggcatt gatcgccaat tccgttgcag ggttacaccg
     4021 aaggtgttgg ccaatttcag cccaggggtt gagctttggc aaccctggga tcacgaatgt
     4081 catttggtgg gaacgtgcaa ccctgaaagg tttgcgcccg ccgcgcgccg cgcgcaaacc
     4141 tgtcgcaatc ccttcaggat tgcgacaaaa acaattcacc atctttatcc ccagggttgc
     4201 caaagctcaa cccctgggct gaaattggcc aacaccttcg gtgtaaccct gcgcggtagg
     4261 ggattttgga tagcgcatga aacaaattgc acatttttgg tgcgataagg tcagaagcac
     4321 tgttcacaag gttgaacggt agtttgtagc gaaatgagta cgtggtggag cgcaggtctc
     4381 aatcatcttc ccgatcgccg tcgtgtctgg tttataagtc ttcgcatcga gtacccactc
     4441 ggatgtgttg gcgtgctaaa ccagcgtgcc cgccacgaca aagcatgaca ggaaagttac
     4501 tcctcgcatc cagctgaccc gagttacctc tcgtcagcct cacacttacc agccgaacaa
     4561 gctgaaagga gatcagcatg gccagagtta ttgaccccac tggcaactcg acgcaaaagg
     4621 cctcaagcga atcgaatccc gagttgaatt ccagaaaggt aatggaagat tttcgtgcaa
     4681 ttcaagcagc agcgaaagca agttctgacg gaaacctttg tgctgtcgcg cccgcccctg
     4741 aacttaagga aaggatctca aaagagattg ccgacatccg ggctaattca aaatcagcaa
     4801 ttgcgtcact ggctcgagcg gcggagccta atagaccagg tttcaatgac ggtctgatcc
     4861 tgccacctga cctgtttcca cttggcactc ctttgagtgc tatccgtaat gccgcgttgg
     4921 accgtgctcc acttcgtggt tccgtccgag tgattgtcgt tctggttgat ttttctgaca
     4981 agcacatgac gcagactcaa gcccacttta gagatttgtt cttctcgacc ggagtaattc
     5041 cgaccggaag tgtgcgtgag tactacaccg aagtaacaca tgggctcatc gatcttcagg
     5101 gagacgtggt tggtccattc cggatgccac tatcgttggc gcaatatgct cacggagcgg
     5161 ccggcatcgg cggggcgagt ccgaatgcgc aaacgatggc actagatgcc gttattgcgg
     5221 cagatccaac cgtaaacttt acgccttttg acaatgatgg aaacggtttt gtagacgctt
     5281 tcattgtggt ccatgccggc gccgggggag aacagaccgg taacccgggt gatatctggt
     5341 cacataagtg gactctggca ggcggcgcca aatctgtcga cgccacaaaa atctttgcct
     5401 atctcaccgt ccctgaagat tcgaagatcg gcgtttgtgc tcatgagttg ggccacctcc
     5461 tcttcggctt tccggatctc tacgacactg accagacttc tgagggaatc ggaaactggt
     5521 gtttgatggc agccggtagt tggggcggcg gcggtgatgt tccagttcat ccgtcggcct
     5581 ggtgtaaagc caaccagggc tgggtgagcg ttgacaacag gaccagtaac agcaccgtca
     5641 cgattcctga tgtcaaagac tccaacacgg tcttgcgact ttggaaagat ggtgcgccgg
     5701 gcaacgagta tttcttagtt gaaaaccggc aaaagaatcg gttcgacaaa tcgctgccgc
     5761 agggcggatt gctgatctgg catgtcgacg attcggtaag cacaaatacc aatgagtcac
     5821 attacaaggt cgctttgatg caggcggacg cgaatcgcga tctggaactt gatcacaatc
     5881 gaggtgacgg aggtgatccg tatcctggct caagtggaaa tacgagtttt aacaagagtt
     5941 catcgccaaa ctcgaagtct tacagtggcg ttgatacctg tgttgccgtt aacgcgatca
     6001 gtgcgtccgg tgcagtgatg acagccaatg tcgcagtgaa gtgtaaggga gtcgaaaagg
     6061 atcaactcaa agatattcga gatgtctttg ataaacgtcg cataaaagag attcgtgaga
     6121 aacggttgga gaaacctcaa atcgataaat tgtattccta cgaaaagggt ttcaccgaca
     6181 aactctcgga taagttcagc gacaaattat cagatggcaa gtttaccgaa ggcggcgggt
     6241 ttccgggagg ttttggcgga ggtttcgcac cccctgatgc acggctaaat caactcgagg
     6301 ctcgagtttc tgctctcgag gcggcggcca tgcaaaagcc cggcgaggaa gctgcgtcac
     6361 cttttatagg cagcgagctt cgaccggact taagtgaagg agcactagca gcagaagacg
     6421 actacagcac tttagatgaa caaatgaggc agggctcagg ccaggccaag cgtttgttcg
     6481 attccaaatt gaaggaacaa taaagtgttg cgttttctga tccttgctca tcgaggagat
     6541 gcgaccgcca tgctcgtggc ttcgcatctc ctcactcgcc atcgacgtga atcagtgcga
     6601 atcgtattag ccgatgagtt cgtgtcgacg gctcaactcg ttcatcgggt aaacggagca
     6661 aaagcgctaa cttcgcttcg actggctgat gggttttcaa tcaagaatga tggcattggt
     6721 attgtcttca accgcttgag atatgtggaa ataccccagt ttgctcgtgc gaatatggta
     6781 gaccaggagt acgcagcgtt agaacttttt gcgtgtttga taagttggat gaagtctttg
     6841 acgtgccctg tttttggagc gccctcgtct caaagtctgg gtggaccaac actcagtact
     6901 cttgcctgga acgttctcgc tggtaaagct ggtttaccta cgtcgcttgt ccggtttacc
     6961 agcagtctga agtactattc gttaaaaggt actgctaaat acccgattgg taattccgta
     7021 aatggcgacg taccggcaat cttgagtcac caatccggag caccaggtat cgctgtggcc
     7081 gttatcggtg agcgagtcat cggtccagtt ccccatgatc tagaagctgg ctgtttaaga
     7141 cttgcaaagc tgtccggctt tgaggtgctg gtggtctatt tttcgaccga agaaaacaat
     7201 ttaatgttcg gcagtgtcga tgcttacccg cagttaacag accctcaaag catcaatgct
     7261 gtggtgaaca ctctcgagga gcgttcttga ttctattctg tggaattcca agtgaacctc
     7321 cgttggcact cgcaatcgaa gctgcgcagc gcctggggct ggaccaggtg gttttcaatc
     7381 aacgccacgc gcagttttgc gacatcgctg tcgacagtca tcgcggaacg ttggctggag
     7441 ctcttcgaat atcagatcga gaatggcgtt tggaagattt ttccggagtc tatacaagat
     7501 tgatcgacgt ccactcgtta ccagaaaatc gaccgcgtcg ttacacgaaa cccgatgacg
     7561 ctgcgatctt gaagagctct ttccttcatc agacgctggc agattggatg gagattgccg
     7621 aatgtcgggt cgtgaacaga gcggctgcaa tgtcatccaa tgtctcgaaa ccctaccagg
     7681 cgcaaatcgt ctcaaaattc ttttccgtgc ctgcaactct ggtgaccaac gatccaggcg
     7741 ccgcacttga attcattcag ctacatagac gtgtgatcta caaatcgatt agttcgatta
     7801 gatcgattgt gcgcgagtgg tccgctgcgg acgagccaag tctctataag attcgttcat
     7861 tgccaacgca gtttcagagt tatgtcgagg gcaccaatat tcgcgttcat gtggttgggt
     7921 ccagaatctt cgcaaccgaa atcatttcga cagcggttga ctatcgttat gcaacacgag
     7981 acgaacttga ggtcgagatg gtcgagacgg aattgccttt ggagattgct gataagtgtg
     8041 tcggattgtc gatggagcta caacttccgt tttgtggcat tgacttgaag agaagtcctg
     8101 acggtagata ctattgtttc gaagtcaatc cgtcacctgc atacagctat tatcaggaaa
     8161 caactggaca gccgattgca gatgccttag tcgaatacct tgcgggtaga accgaggtcg
     8221 atcatgaatg ttcaagctgt tgagaactgg gctttgataa aagggaaggt gttatcaatt
     8281 cgtccgcatc cggaacttga ccggtatgct gtagcgaaaa ttgcagtaga ggaagtcacg
     8341 cctgtcagtg gatatgcgaa tatgtttgcc tgggcagtcg gaaaagaagt cgagtttaat
     8401 attccgcagc cgaaagtcga tgaggtttct ttatctgaag gcgacaagat atccgccaaa
     8461 gtccgcaaag gcacccccac cgcatcgttc gtcgatgccg actcagtcgt caaattaggc
     8521 taacaagaag attccgaact ctgcattcgc tcgcctaatc cgcgggcggg cgtgtatccc
     8581 gtggtgtcct gtttttgaag ttcgcataac aatttagcga gtcattcggt ggactttcag
     8641 cgaggctggg aaagaatctc ccagaaaaaa gcaagagccg atttgttaac aaattggaag
     8701 agggttgggt ttcacccccg tggggtgaca tgtttatagc cgacaactcc caggacactt
     8761 ccactccgtt cgaagttcta agaaggagcg gttagagatc ttgaagcgta cagtccaagt
     8821 cgctccttct tagaacttcg aacggagtgg aggagaggag gggtacgtcc aagtctataa
     8881 acatgtcact cctacggagt gaaaccctgc gaaccaatac atccatcttg tctgttattt
     8941 gaacgctgga ccacaacgaa catcgttaag gctcaagcga aatagtactt tgaaaccgtt
     9001 gtagggctgt taaagatgcc ctttacaact caccggcgcg actaggagga actaatgagg
     9061 tggctcgcga aaattgtcgg agttgggctg tcgattcttt taac
//
LOCUS       DASSFP010000406         6988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1750595, whole genome shotgun sequence.
ACCESSION   DASSFP010000406 DASSFP010000000
VERSION     DASSFP010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750595"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VFH91_09145"
     CDS             complement(<1..1079)
                     /locus_tag="VFH91_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863203.1"
                     /translation="MNDHLSTSQLRGLAVGHLAPGEYEYPAQHIASCEQCHLRFMTQL
                     QDEPTVSSFSLNSEFWFQHDHLTFAQLTSMADDTVDHESLGIIDAHLNSCALCRDSLD
                     SLLTLRDEVQEFKVSRTSANAFPQIKTSVLSNKGIAWHPDFLVAGAILITLATLAGLI
                     VLSKRSKSSVSETAIAVTSPPGNPTSASPGATEGSSGSAVKENELVALLNDGPQAVAI
                     YADGRIEGLRGISLTTSQEVSRAILNGRVKTPNVLMALSAGKGSLRGGNDRTDNFKLI
                     YPSRQVIVETRPRFRWKSLPGVSSYQVYVVNSVGRPVAISEVLPPGQLEWTPQKSLRR
                     GEVFNWTVVAVIDQKEVISPSPSEP"
     gene            complement(1160..2191)
                     /locus_tag="VFH91_09150"
     CDS             complement(1160..2191)
                     /locus_tag="VFH91_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6863204.1"
                     /translation="MDKLLTPYLNSRDEFHTQHNLEELLAVYATPIIRRTMRRRLGFY
                     VSAQGINQQNQDAEDLFQETMTKIVQRLSELRTDSSSEIENFKQYVSRITTNMCIDFL
                     RAKSPARAHLKDSVRELLRRHPDLASWQDEGELVCGLSQWRSSNTIAVFDDASRSIES
                     TSSEFIAARFSDSDIQSAPLSNVVIELLSWIDGPVRLEDLVKVLVSLLHLREPEYESI
                     DELDPNIIDSSAFNESGLEAVEMLMRLWQALQSLPREQRDVFVFGFEDQAGRDLFTAL
                     LLARVATVKELAEVLGMTVEEVIRLRLEMPMDAKSIARKLNTSRTNVSKWRFRALQRL
                     RIELSNKIS"
     gene            complement(2320..2619)
                     /locus_tag="VFH91_09155"
     CDS             complement(2320..2619)
                     /locus_tag="VFH91_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6863205.1"
                     /translation="MGHARARPRRLAEKLLQVRNALVLSQTELVKKLHVQEKISYTRI
                     SDYERNKNEPSLMVLLEYARLAGVHLDVFADDQLDLPARIPGTVNHGNSKSRPKR"
     gene            complement(2902..3246)
                     /locus_tag="VFH91_09160"
     CDS             complement(2902..3246)
                     /locus_tag="VFH91_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863206.1"
                     /translation="METRTDIDVAPFCREDLKALIDAILQPGPHSAISLGHLLLGFRT
                     SIDSGLNGINQTRSALSDAIDFVYLHSPEHTAALNLYRQYLAGELLVNDEPLRLINDA
                     IKRNQPQESSPV"
     gene            complement(3507..4325)
                     /locus_tag="VFH91_09165"
     CDS             complement(3507..4325)
                     /locus_tag="VFH91_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6863207.1"
                     /translation="MNIKAVKDEGDYVLATGDAAAHRLNLLHQTYGPAGRELLLRAGI
                     RPGMRVADLGCGVGMVTQVLAELVGPGGEVIGVDFSTAQIKHARASLGDLAHVKFVEA
                     SATDTRLERESFDLVYSRFLLIHLKEPHVALREMYDLLKPDGILVCEDGDLTSADSEP
                     DSKLKEFSNLFAGLGPCWGVDYSLGRRLMHLVIDANFADPEITFNQPVFAKGENKRLL
                     DLSVAEAGPSFIKAGLISENELEDLLVEMRRLADDDSVVAMLPRVTQIWARKSV"
     gene            4692..6365
                     /locus_tag="VFH91_09170"
     CDS             4692..6365
                     /locus_tag="VFH91_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6863208.1"
                     /translation="MAKPVLLTVDDDREVLRAIERDLRRKYASDYRVLRAESGSAALE
                     IIRELKVRNNPVALFLIDQRMPEMSGVEFLEKAIDLYPDARRVLLTAYADTDAAIAAI
                     NEAGINHYLLKPWDPPQENLYPILDELLEDWQAAFHPQFEGVRVLGHRWSPASHNIKD
                     FLARNHVPYQWLDIETADTDEEVRSLTELISEEEKTNLPLVILTNGVRLLQPSLVDLA
                     GNVGLKTRAAEAFYDLAIVGGGPAGLAAAVYGASEGLRTVMIEREAPGGQAGMSSRIE
                     NYLGFPSGLSGNDLARRAVVQARRFGVEILSAEATALRLDGPYRFLKLADGSEISCHA
                     LLVATGVQWRKLNAPGVERLTGAGIYYGAAMTEAISTKDEDVFIVGGANSAGQAAMYF
                     SRYARRVVMLVRGPSLAASMSQYLINQLKQTNNIQIELNSSVIEAHGEDHLEAISVKC
                     TVTDEVNKHPANSLFVMIGAAPNTEWLANIVERDERGFILSGPMLMRDGKRPKGWLVD
                     RDPALLETNVPGIYVVGDVRQGSVKRVASGVGEGSVAISFVHQYLSKVV"
     gene            6367..>6988
                     /locus_tag="VFH91_09175"
     CDS             6367..>6988
                     /locus_tag="VFH91_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863209.1"
                     /translation="MNETKMEGVGVPIPVSGAIESFGPTLTSPENRVEALRRVSVFAD
                     LPDDQLQWFADHTEERRFQPGEILFHKGEPAVWMTIYLEGEIHAYLADKAHDGYVYIA
                     RAGERATEVSGLLPFSRMVTWGATGHAVTHARVLLFPASLFPELFQRMPTLVQRLVGL
                     LSDRVREVTTADQQRDKLMALGKLSAGLAHELNNPAAGATRAADDLM"
BASE COUNT         1654 a         1664 c         1864 g         1806 t
ORIGIN      
        1 tcaggctcgg agggcgacgg tgagatcact tctttctgat caatgacggc aactacagtc
       61 cagttgaata cttcaccgcg ccgtaaagac ttttgaggag tccattccaa ttggcctggt
      121 ggcaaaacct cgctgatggc aactggtctg ccaactgaat tcaccacgta gacctgataa
      181 ctactaactc ctgggagact cttccagcga aatcgtggac gcgtctccac tatgacctgg
      241 cgcgacggat aaatcagttt gaagttatcc gtgcggtcat tgccgccacg taaagagcct
      301 ttgccggctg agagtgccat caaaacgttg ggagttttga cacgaccgtt aagaatcgct
      361 cgcgagacct cttgggaagt ggtcaatgaa atgcccctga gtccctcgat gcgtccatcc
      421 gcgtagatcg cgaccgcttg tggaccatca ttcaatagcg ccactaattc attttctttg
      481 acagctgagc cagaggatcc ttcggttgcg ccaggtgagg cggacgttgg gttacctggt
      541 ggagacgtta cagcgatcgc ggtttcggag acagaagatt tggacctttt gctgagcacg
      601 atcagacctg ccaacgttgc cagtgtgatc aggatagcgc ctgcgaccag aaagtcgggg
      661 tgccaggcga ttcctttgtt agacaatacg ctcgtcttga tttgaggaaa ggcgttggcg
      721 gaggtccgag acactttaaa ttcttgcact tcatcgcgaa gtgtcagaag gctatccaaa
      781 ctatcgcgac atagtgcgca tgaatttagg tgtgcatcga tgattcccag cgattcgtgg
      841 tcaaccgtgt catccgccat gcttgttagt tgcgcaaagg ttaagtgatc gtgttggaac
      901 cagaactcgg aattcaaact gaaactggag accgttggct cgtcctgcaa ctgcgtcata
      961 aatcgcaaat ggcattgttc acatgaagct atgtgctggg ccgggtattc atactcgccg
     1021 ggtgcgaggt gtcccacggc caggcctcgt agctgagatg tcgaaaggtg atcgttcaca
     1081 cgtgaaatat ttggtctatt accagagtcg ctgatgtccc tgactataaa gacgagagtc
     1141 tgggctcaaa attccacgtt cacgaaattt tattgcttaa ttcaatccgc aaacgttgca
     1201 gtgcgcgaaa acgccactta gaaacgttcg tgcgcgaagt attcagtttc ctcgcaatgc
     1261 tctttgcatc cataggcatt tcgagacgca gccgaatgac ctcttcgaca gtcattccca
     1321 acacttcagc cagctctttg actgttgcaa ctcgcgccaa taaaagtgcg gtgaacagat
     1381 cgcgtccagc ctggtcttcg aatccaaaca cgaaaacgtc gcgctgctcc cggggcaacg
     1441 attgcagtgc ctgccacaaa cgcatcaaca tttcaaccgc ttctaacccc gattcattaa
     1501 atgctgaaga gtcaataatg ttcggatcca actcatcaat tgattcgtat tcgggctctc
     1561 gtaagtgcag caatgaaacc agcactttca ctagatcttc gagccgaact ggtccgtcaa
     1621 tccaggagag caactcgatt acgacatttg atagtggcgc gctttgtata tctgagtcgg
     1681 aaaatcgtgc ggcaatgaat tcgctcgagg ttgactcgat gctccgagag gcatcgtcaa
     1741 atacagcaat tgtgttggag gatctccatt gcgaaagtcc gcaaaccagt tcaccctcat
     1801 cttgccatga agccaggtcg ggatgccttc ttaataactc tcttacactg tctttaagat
     1861 gggctcgcgc tggtgatttt gcgcgtaaga agtctatgca catattggta gtaatccgac
     1921 tgacatactg cttgaagttt tcaatttcgg atgaggaatc tgtccggagt tctgacaggc
     1981 gttgaacgat ttttgtcata gtctcttgaa aaaggtcttc tgcgtcctga ttttgttgat
     2041 taatcccctg agcgctgacg tagaaaccta aacgccgtct cattgtcctt ctgataattg
     2101 gggtggcata cacggcgaga agttcttcga gattgtgttg agtgtgaaac tcatctcggg
     2161 aattgaggta aggagtcaac agcttgtcca tgaggtgcct tacttttcga aacagcttcg
     2221 gtgacttttg attgagctta cgttttcaaa agctgggagg acgttgctgt cgaagtggcg
     2281 aatagcgttc tggacgttcg caattcaccg agtgagtttt taacgcttcg gacgtgattt
     2341 gctgttgcca tggttaaccg tacccggtat gcgagcgggc aggtcgagct gatcatcagc
     2401 aaagacgtcc agatgtacgc ccgcgagtcg tgcgtactcc agaagaacca ttagcgaggg
     2461 ctcattctta tttcgttcgt agtcagaaat tcttgtgtag gagattttct cttgaacgtg
     2521 cagttttttt acgagttcag tctgagacag aacgagagca tttcgcactt gaagtagttt
     2581 ttcggcaagc cgtcgcggcc gcgctcgtgc gtgacccatt agctgcgacc tccctctgat
     2641 atggagaccg cgaaacttac ggggtacgtt gttgcggata gcggatagct gcagccggga
     2701 atcgccgtga gtttgagtct tacatgctca ccggcggtgc tcatctctca acttggaggg
     2761 cgagctacgc attacgtgtg cacttgcaaa aattttgggg cgactaagat aacactgaca
     2821 tccgctgtgg ggaaattatt ttcaactcct tccttgatgg ctgagctggt tgtgtttgtg
     2881 tgagactggg gtctgaggag ctcaaacggg gctggactcc tgtggttgat tccgttttat
     2941 tgcatcgtta attagtcgca atggttcgtc gttgaccaaa agttcgccgg caagatactg
     3001 gcgatataga tttagagccg cagtatgttc gggagaatgg aggtaaacaa aatctatcgc
     3061 atcactcaat gcgctgcgcg tttggttaat tccattaagg ccgctatcga tcgaagtgcg
     3121 aaaacccagc agaagatggc caagcgaaat tgcagagtgc ggaccaggtt gaaggattgc
     3181 atcgattaag gctttcaaat cctcacgaca aaaaggagca acatcaatat cagtacgtgt
     3241 ttccatatcc actctctctt gaaagttatg tgcgcgctct tttcactccg ttcagtatga
     3301 tccgcggaac agttattcac cgttgggacg cgaaggagac agagatttcc cagtgagtat
     3361 gagctttatg tccctactcc ctaaagagaa ccgcagtgct tgttcaatcg acacataata
     3421 atatttcttc gagcggatta atatctcgat tagttgttct ggatggctat ttattgacta
     3481 atctggctac ggcctttgta aaacattcaa actgattttc gggcccatat ctgagtgacg
     3541 cgtgggagca tcgcgactac tgagtcgtcg tcggcgagtc ggcgcatttc tactaacaag
     3601 tcttcgagtt cgttctcact gatcaatccg gcctttatga atgagggacc agcctcggct
     3661 actgagagat cgagaagacg tttattttca cctttcgcaa agacgggttg attgaaagtg
     3721 atctctggat cggcgaagtt tgcatctatc actagatgca tgagacggcg tcccaaactg
     3781 taatctactc cccaacatgg tccaagaccg gcaaacagat tcgaaaactc tttcaatttg
     3841 gaatcaggct cgctgtcagc cgatgttaga tcaccatctt cacaaacaag aattccgtct
     3901 ggtttcaaca aatcgtacat ctcgcgcagt gcgacgtgtg gctctttgag gtggatgagg
     3961 agaaatcggg aatagaccag gtcaaacgat tctcgttcga gtcttgtatc ggtggcactc
     4021 gcttctacga acttgacgtg cgctaaatcg ccaagtgagg cacgggcatg tttgatttga
     4081 gcagtactga agtcgacgcc aataacttct ccgccgggac caacgagttc cgccagtact
     4141 tgcgtcacca tcccgacgcc acatccgaga tcggccactc tcatcccggg tcgaattcct
     4201 gctcgtaaca acagttcacg gccggctggg ccataagttt ggtgaaggag attcaggcga
     4261 tgtgcggccg catctccggt cgcgagtacg tagtctcctt catctttgac ggctttgatg
     4321 ttcatcctct cgttagttgc gacccattat tctacatggc tttgagaaat ggcataacag
     4381 ttagtgtccc aatctaaagt ttctctttga tggtgatcca gggggttttc tgcgctgcac
     4441 cccttggcta ccttctcgcc gccccttcgg ggctcgggtg gtgtagcgct ggtggtggaa
     4501 taaacctggt ttgctgaaat ttgcgagaat gttgtctgga tttttgccgc tttgcgcaaa
     4561 gaactaattt tcaactactc ttaatctcta aatccatgaa gatgtctgct cgcgtttgat
     4621 aggtttaaac cgaggaaagc tgtggatcac gaacaactga accacgaaca attgaactaa
     4681 agagaataaa tatggcgaaa ccggtattac ttactgtaga tgacgataga gaagttctcc
     4741 gcgcaatcga acgagacctg cgtcggaaat atgcaagcga ttatcgggtc ctccgagcgg
     4801 aatccggatc agctgcgttg gaaatcattc gagaactaaa ggttcgcaat aatcctgtcg
     4861 ctctttttct tattgatcaa agaatgccgg agatgtcggg agttgagttt ttggaaaagg
     4921 cgatcgacct ttatcccgat gccaggcgag tgctcttaac cgcttacgca gatacggacg
     4981 ccgcaatcgc tgccattaac gaggctggta ttaatcatta cttactcaag ccgtgggatc
     5041 cgccccaaga aaatctctat cctattctgg acgagttgtt agaggattgg caggctgctt
     5101 ttcatccaca gtttgaagga gtgcgcgtcc tgggccatcg ctggtctccc gcctcccata
     5161 acatcaagga ctttctggca cgtaaccacg ttccatatca atggctggat attgagaccg
     5221 cggatacgga tgaagaagtg cgcagcttga cagaattaat cagtgaggag gaaaagacaa
     5281 atcttccgct ggtgatcctg acgaatggtg tgcgtttact tcaaccttct ctcgttgacc
     5341 ttgccggtaa tgttggtctc aagactcgcg ctgcggaagc tttttacgat ctggccattg
     5401 tgggaggtgg accagctggt cttgcggctg cagtttatgg cgccagcgaa ggtttgcgaa
     5461 cagtgatgat cgagcgagaa gctccgggcg gtcaggccgg gatgtcgagt cgtatcgaaa
     5521 actatctagg gtttccgagc ggactcagtg gaaacgacct ggcacggcgc gcggttgtac
     5581 aggcgaggcg gtttggagtc gagatcttgt cagcggaggc taccgctctg agattggacg
     5641 gtccttatcg atttctcaaa ttggccgacg gctcggaaat aagttgccat gccttgctgg
     5701 ttgcaacggg agtgcagtgg cggaaattga atgctccagg cgtcgaacgc ttaaccggtg
     5761 ctgggattta ctatggcgct gcgatgacgg aagcaatctc aaccaaagat gaggatgtgt
     5821 ttatcgttgg cggcgcaaac agtgccggtc aggcggccat gtacttttca agatatgctc
     5881 gtcgagtcgt aatgctcgtg cgaggaccat cgctcgctgc ttcaatgtcg caatatttaa
     5941 ttaaccagct caagcagacc aacaacatcc aaattgagtt gaactcctcc gtgatcgagg
     6001 cgcatggtga agaccattta gaagcaatct ccgtcaagtg tacggtgact gacgaggtta
     6061 acaagcatcc cgccaactct ctgttcgtca tgattggcgc cgcgccgaat acggaatggt
     6121 tggcgaatat agtagaacga gacgagcgag gcttcatact gtctggtcca atgcttatgc
     6181 gcgatggcaa acggccgaag gggtggttag tagatcgtga tcctgctttg cttgaaacca
     6241 acgtgcctgg catttatgtg gttggcgatg ttcgtcaggg ttcggtaaag cgagtcgcgt
     6301 ctggcgtcgg cgaagggtct gttgcgattt ccttcgtaca tcaatatctc agtaaggtgg
     6361 tctaagatga acgagaccaa aatggagggc gtaggcgtac cgattcctgt gtcgggcgcg
     6421 atcgagtcat ttgggccaac gctcacctcg ccggagaaca gggttgaggc gctccgtcgc
     6481 gtttcagtgt ttgcggattt gccggacgat cagttgcagt ggttcgcgga ccacactgaa
     6541 gagcgacgtt ttcagcccgg agaaatcctg tttcacaaag gtgaaccggc tgtctggatg
     6601 acgatctatc tggaaggaga gatccatgcg tatttggctg ataaggccca tgacggatat
     6661 gtgtatatag ctcgcgcagg agagcgcgcg acagaggtga gcggactgct gccgttttcg
     6721 cgcatggtga cctggggcgc taccggacat gcagtcactc acgcaagagt tcttctcttt
     6781 cctgcgagct tatttcctga attattccag cgaatgccga cgctggtcca acgcctggtt
     6841 ggcctgttga gcgatcgtgt gcgtgaagtg acaacggcgg accagcaacg cgacaagcta
     6901 atggcgttag gtaagctctc ggcgggactt gctcacgaac taaacaatcc tgctgcgggg
     6961 gcgacgcgtg ccgcggacga tctcatga
//
LOCUS       DASSFP010000407         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1750939, whole genome shotgun sequence.
ACCESSION   DASSFP010000407 DASSFP010000000
VERSION     DASSFP010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750939"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..1409
                     /locus_tag="VFH91_09180"
     CDS             444..1409
                     /locus_tag="VFH91_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863210.1"
                     /translation="MLYSAFGLTLKTNRDIPGLIRSPVNDADVQIIMGEFPDWFRDHS
                     SAQLYFQNKKVDSNGAALLTVWKLKENYFHFRYSDNIEFLVNESNVFARWPASLTLED
                     TVVYLLGPILAFVLLLRGIVTLHASAIAIDNYAIALIGPAGSGKSTTAAGFAQKGFSV
                     LSDDVTILDDRGNEFYVTPTYPRIRLWPSSVGNLCGSEDALPLLTPTWDKRFLDLTTN
                     DKFEADSLPLKALYFFADRVGEDDRPKIISCSAPEGLTGLVSNAYVTYLKDRNKRSQE
                     FQLLNKLIAKVPVRSVLPHSDPRYLDRLCDVLIQDFRQMELVGNV"
     gene            1402..2541
                     /locus_tag="VFH91_09185"
     CDS             1402..2541
                     /locus_tag="VFH91_09185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6863211.1"
                     /translation="MYSVSGYGEMIADRVRTRAYLEALKQTVKPESVVLEIGSGLGLF
                     AISAAQSGASKVYALEPDDVIELSRESARAANVADKITFLQALSTNVELPEQVDLIIS
                     DLRGVLPLFLNHIPSIIDARNRFLKPNGVLIPSLDRVWAGPVEAPEHYKKIDMWQDLQ
                     RDIDCTSARRIASNTWWKARARIEQLLDRPQCLTTLKYNFISSPNITGNLGFEISRDG
                     VLHGWALWFDTNLFGEIGFSNHPEEVELIYGNAFFPLTEAIEVQKGDTINLSFRANLV
                     NDDYVFSWNTEVYNSRDLEKRSFRQSTFFGVPFSANNLSKQATTHMPKLSMQGQIDRQ
                     VLTLMEDGRTIDEIAEVLTSEFPQYELTKDKALALVSKLSRIYSI"
     gene            2558..>2920
                     /locus_tag="VFH91_09190"
     CDS             2558..>2920
                     /locus_tag="VFH91_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863212.1"
                     /translation="MIGAITLTKSGPDITTDHRVIEDLRTQFSTRSFVYLRQLIAPDL
                     LELLSNNIESSSFQRLEHKRVGAELGTPDFKTGAVIGVLFNNAKLFEFVRTITDCGSI
                     GSFIGRVYRYLPDEDHYDA"
BASE COUNT          764 a          688 c          690 g          778 t
ORIGIN      
        1 tgaggatccc tggtcaaaaa tggagagtgt tcacgagtcg ctatctggtt atgtggattt
       61 gaatcagttt aaaagctgcg tggcagatgg gtccgaaaat ttgtgggtcg atatgcggcc
      121 cataagttta aacctttggt tgcaacacaa ggaatgatgc tataatcccc cgcccaaagc
      181 agtgactact cccctactag tgcagtttgg atctcgtcgc tgcttcaaaa ttcgcagctg
      241 aagtaactca cctactaatt ggagagaaga atgacagctg agaagtcaaa aacgcccaga
      301 aaagaatata ggaagcctgt cttagtaacc tatggtagcg tcgaacaatt aacaaacgca
      361 tcaggaatga cgtcaatggg cgcagatggc ggtcctggcg ccagcactaa aacctcttaa
      421 agtcacgtga cgggttcgat gttttgcttt attcggcttt cggacttacc ttaaaaacaa
      481 atcgcgacat tcccggccta attcgctctc ccgtcaatga tgctgatgtc cagatcataa
      541 tgggagagtt tcccgattgg ttccgggatc actcttccgc tcagttatac ttccaaaaca
      601 agaaggttga ttccaatggt gcagcccttc tcactgtttg gaaacttaaa gagaattact
      661 tccattttcg gtattccgat aacatcgagt ttcttgttaa tgaatcaaac gttttcgctc
      721 gctggccagc tagcctgact ttggaagata cggtcgtcta ccttttgggg cccattctcg
      781 cctttgttct tcttcttcgc ggaattgtta ctctccacgc gagcgccatc gctatagata
      841 actatgcgat agccttgatt ggacccgccg gatcaggtaa gtcaacgacg gccgccggat
      901 ttgctcaaaa gggcttttca gtactctccg atgacgtgac aattctcgac gatcgtggaa
      961 acgagtttta tgttacgcct acctacccac ggatcagatt atggccatcg tcggtcggga
     1021 atctgtgtgg ttctgaagat gcgctgccgt tgctcacgcc gacctgggac aaacgctttc
     1081 tggacctgac tacaaacgac aagttcgaag cagattctct accactaaaa gcattgtatt
     1141 ttttcgctga ccgagtgggt gaggacgatc gtcctaaaat aatttcatgc agtgcgccgg
     1201 agggactgac cggactggtc agcaatgctt acgtcaccta cctgaaagat aggaataaac
     1261 ggtcgcagga gtttcaattg ctcaacaagc tgatcgcgaa ggtgccagtc agatcagtct
     1321 taccacacag cgatcctcgc tatctcgacc ggctttgtga tgtattgatt caagatttcc
     1381 ggcaaatgga gcttgtcggc aatgtatagc gtttccggtt acggagagat gatcgcggat
     1441 cgagttcgaa cccgggctta ccttgaggct ctaaaacaaa ctgtcaaacc ggagtccgta
     1501 gtccttgaga tcggatctgg tctgggtctc ttcgcaatat cggctgcaca gtcgggagcc
     1561 agcaaagttt atgcactcga accggatgat gtaatcgaac tcagtcgaga gagcgctcga
     1621 gccgcgaatg tggctgacaa aataacgttt ctgcaagcac tatcgacgaa cgttgaactt
     1681 cctgaacagg ttgatctgat catctctgac ttacgaggtg tgttgccatt atttctcaac
     1741 cacattcctt caatcatcga tgccaggaat cgctttctaa aaccaaacgg tgttttgatt
     1801 ccttccttgg acagggtttg ggctggtcca gtggaggcgc ctgagcacta taaaaagatc
     1861 gatatgtggc aagatctgca acgtgatatc gactgcactt cagcgagacg gatcgcgagc
     1921 aatacatggt ggaaggcacg ggctagaatc gaacaattgc ttgatagacc tcaatgcctg
     1981 accacgctca aatacaactt catttcttcg ccaaacataa ccggcaatct tggctttgaa
     2041 attagtcgag atggtgtctt acatggttgg gccctatggt ttgatacgaa cctgtttgga
     2101 gaaatcggat tctcaaacca tccggaagaa gttgagctga tttatggaaa tgcgttcttc
     2161 ccgttgactg aagcaatcga ggttcaaaaa ggagatacga tcaatctttc ttttcgtgcc
     2221 aatctggtaa acgacgatta cgtgttttca tggaatacag aagtttacaa ttctcgagat
     2281 ctcgaaaagc gttcttttcg ccaatcgacc ttctttggtg tgcctttttc tgctaataac
     2341 ttaagcaaac aagcgacgac tcatatgcct aaattatcta tgcagggaca gatcgatcga
     2401 caggttctga ccctgatgga agacggcagg acaatcgacg agatagccga agttcttacg
     2461 agtgaatttc ctcagtacga gttgactaaa gacaaggcgc tcgcgctggt aagtaaattg
     2521 tcgcggatct attcgatctg acgataacga tgaaatcatg attggagcta ttaccttaac
     2581 aaagagcgga ccagacataa ctaccgacca ccgtgttatc gaagacctgc gcactcaatt
     2641 ttccactcgg tcttttgttt accttcgaca gcttatcgcc cccgatcttc tcgaactttt
     2701 atccaacaac attgaaagca gtagtttcca acgtcttgag cacaagcgtg tgggagccga
     2761 actcggcacc ccggatttca aaacgggcgc tgtcatcgga gtgttgttca ataatgcaaa
     2821 gttgttcgag ttcgtgagga cgattacaga ttgtggctca atcggatcct ttattggtcg
     2881 cgtctatcga taccttcccg atgaagacca ttacgacgct
//
LOCUS       DASSFP010000408         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1153246, whole genome shotgun sequence.
ACCESSION   DASSFP010000408 DASSFP010000000
VERSION     DASSFP010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153246"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1862
                     /locus_tag="VFH91_09195"
     CDS             213..1862
                     /locus_tag="VFH91_09195"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET6863213.1"
                     /translation="MKTRLILFLLIFIVLSGHGLQYAIGQQPANSPARADILRGEYGR
                     FRANNDLLYYELSVRVDPEQKSLKGHTLVRFKMLRDDNRIQLDLHQALNIDKIVLLTS
                     GVPSSPLKFEREDGAVFVDFPEVLKAGRTYDVDFYYSGHPLETGRFGGIAFRKDPYGR
                     HWINTACEGEGASIWWPNKDQWRDEVESMRISVEIPKDLVDVSNGRFVGKTDLGDGYT
                     RWDWLVNYPINNYNVSLNIGRYEHFSDKLGDLTLDFYALPEDLDKAKKQFVQARGMLE
                     AFQHYFGEYPFKKDGYKLIQAPYSGMEHQSAVTYGNHFANGYLERDWTGVGISPRFDF
                     IIIHESGHEWFGNSVSAADTSDMWIHEGWTTYLECLYVEYMYGKADGLKYTNAYKSKV
                     QNRRPIIPQRGINATPPQDMYFKGALFLNTLRSVIDDDKRWFALIRDVYQHFKYRNIM
                     TEEMVTFFNQKTGRNLTPIFNQYLRHTSLPTLELKFSEGAVGYRWKVDEANFNMPVRV
                     GSKDRWQLVQPTTEWQTLNTNIKKDEFDVATDLYFIEVQKD"
     gene            1900..2784
                     /locus_tag="VFH91_09200"
     CDS             1900..2784
                     /locus_tag="VFH91_09200"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET6863214.1"
                     /translation="MYVITGATGNTGHVIATRLLDAGQKVRVVGRSRERMQHFAEQKA
                     EIAVADLRDESAVITAFSDATAVFAMIPPNMTSQNYRAEQDQTSDVLANAIKRTGVPN
                     VVSLSSVGADKHDGTGPVDGLHYLEQRLNEIADLNVLHLRPAYFMENTLAQVTAIRLM
                     GATAGPIHADLRIPMIATRDIGVAAADALLRLDFRGKRTKELHGERDLSYNEATSIIG
                     KAIGRPDLAYKELPPSEFQAALEQMGASANVASLIAELGNALNSGHIKMLEPRSSSTT
                     TATSYEAFVADVFVPAFG"
     gene            2909..3391
                     /locus_tag="VFH91_09205"
     CDS             2909..3391
                     /locus_tag="VFH91_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863215.1"
                     /translation="MSAIALHDRRPAALQAILLSGATAGVLDLTAACVFAWIRSNVRP
                     VLVFHYIASGAMGPSAYQGGAKTAALGIAFHFLIAITAAAVYYFASQKMSLLVTQPIF
                     SGVLYGIFVYLFTNFVVVPLSLVQRRPQPLSSRIIAALIIIFCVGLPIAFITRRFARR
                     "
     gene            3411..>3727
                     /locus_tag="VFH91_09210"
     CDS             3411..>3727
                     /locus_tag="VFH91_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6863216.1"
                     /translation="MNLQQREMRQEDVETCGKICYHAFKQISDAHNFRPDFPNEQAGI
                     EFTQALLDSPHVFSVVVEDDGKVIGANHLWEYDAIRAVGPITVDPRAQAKGIGRILMQ
                     AVL"
BASE COUNT          963 a          896 c          957 g          911 t
ORIGIN      
        1 ccgggtcacc aaattcaaaa cgaaccggaa aagctgtcgg acatcaaaac ttatgtacgc
       61 aacttagcgc tctggcttac acctcaaaga aattgaaggg tttgcaccgg gtttaggctg
      121 actgtttgca ggatgatcga taactcaact atccaactgg atgtcgttgg tttcggtcga
      181 aatcatattg atgtcaaagt ggatctcaat taatgaagac acgccttatt ctctttctcc
      241 tcattttcat tgttttatct ggacacggac tccaatacgc cattggccaa cagcccgcca
      301 actcgcccgc acgggctgac attcttcgag gtgagtatgg tcgattccga gcaaataacg
      361 atctgctcta ttacgaactg tccgtgagag ttgacccgga acaaaagtcc cttaaaggac
      421 atacactcgt tcgtttcaaa atgctgagag acgacaatcg aattcaattg gatctgcacc
      481 aggcgttgaa tatcgacaag atcgtcttgc taacatctgg agtcccgagt tccccgctca
      541 aatttgaacg agaagatgga gcagtttttg ttgacttccc ggaggtgcta aaagcgggac
      601 gcacttacga cgttgatttt tactactcgg gtcacccgtt ggagactggc cggtttgggg
      661 gcatcgcttt tagaaaggac ccttacggtc gtcattggat caacacagct tgtgagggcg
      721 aaggcgcttc catttggtgg cccaataagg atcagtggcg tgacgaagtc gagagcatgc
      781 ggatcagcgt tgaaatccca aaggatctcg tcgacgtttc gaatggaagg ttcgtaggaa
      841 aaacggatct cggcgacggg tacacacgtt gggactggct cgtcaattat ccgattaaca
      901 actacaacgt ttcgctaaac attggtcgtt acgaacactt ctccgacaaa ctcggtgatt
      961 tgactctgga tttttatgca ttaccggaag atctcgacaa ggcaaagaaa caatttgtcc
     1021 aggcgcgagg gatgctagag gcgtttcaac actattttgg cgagtaccct ttcaagaaag
     1081 atgggtacaa actgattcag gctccgtatt caggaatgga gcatcagagc gccgttacgt
     1141 atggcaacca ctttgcgaac ggctatctcg agcgcgactg gacgggcgtg ggcatcagtc
     1201 ctcgcttcga cttcataatc atccatgaga gtggtcatga gtggtttggt aatagcgtct
     1261 cggctgccga tacttcagac atgtggattc atgagggctg gaccacttac ctcgagtgcc
     1321 tgtatgtcga atacatgtat ggcaaagctg atggcctgaa atatacaaat gcctacaagt
     1381 cgaaggtcca gaatcggcga ccgattattc ctcaacgcgg gattaatgca acacctccac
     1441 aggacatgta cttcaaagga gcactgttct tgaacacttt gcggagtgtc attgacgacg
     1501 acaaacgttg gtttgcgcta atacgtgacg tctatcagca ttttaagtac aggaacatca
     1561 tgactgagga gatggtcaca ttttttaacc agaagacagg ccgcaacctg actcctatct
     1621 tcaatcagta tcttcgtcat acgtcgttgc ctacgctgga gttgaaattc tctgaagggg
     1681 cagtcggtta cagatggaag gtggacgagg ctaacttcaa catgccggtg cgggttgggt
     1741 ccaaagatcg ctggcagtta gttcaaccca ccacggaatg gcaaactcta aacacaaaca
     1801 tcaagaaaga cgagttcgat gttgcaaccg atctgtattt catagaagtg cagaaggatt
     1861 aggcagcaca aacgatcggg aaaggattag aggtatcaga tgtacgttat aaccggggct
     1921 acaggaaaca caggtcatgt tatcgcaaca cgtttgctcg acgccgggca aaaggttcgg
     1981 gtggttggca gaagtcgaga gcggatgcag cacttcgcgg aacagaaagc ggaaatagca
     2041 gttgcggact tgcgagacga aagcgcggta atcacggcat tcagtgacgc tacagcagtc
     2101 ttcgctatga tcccgccaaa catgaccagc cagaactacc gggctgaaca ggatcaaact
     2161 tcggatgtcc tggcaaacgc tattaagcga actggtgttc ccaatgttgt gtcgctcagt
     2221 agcgttggcg ctgataagca tgacggcact ggaccagttg atggtttaca ttatctggag
     2281 caacgcctaa acgaaattgc cgatttgaat gtgctccatc ttcgtccggc gtacttcatg
     2341 gagaacacac ttgcacaagt gacagcaatt cgtttgatgg gggctactgc aggaccgatc
     2401 catgcggatc tgcgcatccc gatgatcgct actcgggata ttggagttgc cgcagcagac
     2461 gcgctgctaa gactcgactt ccgcggtaag aggacgaagg agttacacgg cgaacgcgac
     2521 ttaagttata acgaggccac gtcgattatt ggcaaagcga ttggccggcc cgatctggct
     2581 tacaaggaat tgccaccgtc agaatttcag gcggcgttag agcagatggg tgcgtctgcc
     2641 aatgtagcga gtttgatagc tgagttagga aacgcgctaa attccggtca catcaagatg
     2701 ttagagccac ggtcctcgag cactacgact gcaacatctt acgaagcgtt tgtggctgac
     2761 gtgtttgtgc cggcattcgg ctgaacgagc gttacagaac ctcgagcgaa gcgactgcac
     2821 tcaaaattga agtgcagcgc acatcgctca tgttgtacaa aagcaccaag cgcattacac
     2881 aacttcgcct gaaaggaccg ttcctgctat gagtgctata gcgcttcatg atcgcaggcc
     2941 tgcagctcta caagccattc ttctgagtgg cgcaactgcc ggcgttctgg atttaactgc
     3001 cgcgtgtgtc tttgcctgga tacgatctaa cgttagacca gttcttgtgt ttcattacat
     3061 cgcgagcgga gccatgggtc cctcagcgta tcaaggcgga gcaaaaactg ccgctttggg
     3121 aattgccttt cactttctta tcgccatcac ggctgccgca gtttattact ttgccagtca
     3181 aaagatgtcg ttgctggtca cgcaaccgat cttttcaggt gttctctacg gaatctttgt
     3241 atacctgttt acgaacttcg tcgttgtgcc tttgtcgctc gttcagagaa gaccacagcc
     3301 actctcgtcg agaattattg ctgcattgat aataattttc tgtgtgggcc taccgatcgc
     3361 tttcatcact cgccggttcg ctcgtcgtta gtgccagctt tggagtcaaa atgaatctgc
     3421 aacagcgcga aatgagacag gaagacgtcg aaacatgcgg gaagatctgc tatcacgctt
     3481 tcaaacaaat ttcagatgca cataacttcc gtcccgattt cccaaatgaa caagcgggca
     3541 tcgaattcac tcaagctcta ctcgatagcc ctcatgtctt cagtgtagtg gtggaggacg
     3601 atgggaaagt aatcggcgca aatcacctct gggaatacga cgctattcgc gcggttggac
     3661 caataaccgt cgatccacgc gcgcaggcca aaggtatcgg tcgcatcctg atgcaggctg
     3721 tcctgga
//
LOCUS       DASSFP010000409         5566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_268809, whole genome shotgun sequence.
ACCESSION   DASSFP010000409 DASSFP010000000
VERSION     DASSFP010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268809"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..2105)
                     /locus_tag="VFH91_09215"
     CDS             complement(27..2105)
                     /locus_tag="VFH91_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6863217.1"
                     /translation="MNSNTQLAPDLKLIAPWHLRAFPNREWILILVLVLECGIFSLSG
                     DNFLTRSNAFEITRLSVEVGLLALALTPVIITGGIDLSVGSMMGLSAVVLGSLWRDGH
                     WPMSLAIVAVLILGLLGGALNGMLIARIGLPPLIVTLGTFSLFRGVAEGLTRGIENYS
                     GFSSSFLFLGQGYLGHLVPTQLVVLIIGIALCWWCLHRTAFGRTLYAIGHSPEGARYA
                     GIPVGKNLFIVYLISGLAASISAVVYVAHLGQAKSDAGTGFELMAITAVVLGGASIFG
                     GRGTVLGTVLGLFAIVVLQNGLRLSRQPAELAGILTGALLLITLILNRLANFVGGQGQ
                     VNAGAEEFDVKNSQVAILSGVILLAALLVAGSNWWLVRSIQNGRQSEQPLNSATNSNH
                     RIQVAMMPKAKGDPYFVSCRQGAEEAAKELGIDLLWDGPTDLDPAKQNELVEAWITKG
                     VDVIAVSVENQAGISTVLRKAREKGIKVLTWDADAERNARDFLINQATPQGIGYTLTD
                     EAARILGNKGEFAVITASLSAANQNEWIKFIRARLAEKYPDLKLVTVQPSEGDRDRAF
                     SETQTVLKVYPNVKLIMAIAAPAVPGAAEAVKQAGRTDVKVTGLSLPNMNKPYVKDGV
                     VESIVLWNTTDLGYLTVYAAQALITGQLKQGAHQLVAGRLGKIDVVDDEVRLGSPFIF
                     NKENIDRFDF"
     gene            complement(2116..3087)
                     /locus_tag="VFH91_09220"
     CDS             complement(2116..3087)
                     /locus_tag="VFH91_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6863218.1"
                     /translation="MNRTSLITRHKREFSVALSYLALLVLVALLAPSFFQPANLRDLA
                     INNASVLIVACGMTLVILTGEIDISVGSQFAVCTYAAGLLAKNGVPVVLLIPAVILIG
                     AAMGAVGGILVARLGIPSIVATLALMVIWRDALRWGSEGAWIQSLPQNFQWFGLRQGH
                     GEMLLVIAALIVFASIAWSLRRLGAGRFFYAVGSDSEAARLAGIDSQLVKLNAFVIMG
                     ALTGLAALLNAIRFSIVPGNAGIGLELKAIAAVVVGGTAITGGRGTVAGTLIGVVLLG
                     TIGPALTFVGINPFWEKAIQGTIIIAAVVSDVAMTQFSARRQYRLAN"
     gene            complement(3102..4625)
                     /locus_tag="VFH91_09225"
     CDS             complement(3102..4625)
                     /locus_tag="VFH91_09225"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6863219.1"
                     /translation="MDQHNSNSRVILRLKNIVKSFDGVAALKGVAFDLREGEVHALVG
                     ENGAGKSTLVKTITGAVVHDRGSVEFDGQRIFDHSPAHAKALGIAAIYQQPALFPELT
                     VTENISLGIEKPRTWRRIDWKARRARAIELLQRVGSGIDPDVDVQSLSMPEQQLVEIA
                     RAVGANSRLLIMDEPTASLAEEETQNLYRVIDALKKDGVAIIYISHRLEELEAVADRV
                     TVLRDGVIVDTRMMSEVSRNDLIRLMVGRELSAVFPKREVGIGKAFLETKGLSCRLNG
                     VENIDLTVRAGEIVGLAGLVGAGRTQLARTIFGLEPADRGEIRINDQTVRIDKPSTAI
                     QLGIAYLPEDRRRHGVMLDMPIDANVTLASLDKLRKGLTFDRASERDIAEKYVRRFAI
                     KAPALFSAVTTLSGGNQQKVALSRWLETRPALLILDEPTQGIDVGAKAEIHSLMGELA
                     AQGAAIIMISSDLPEILGMSDRIVVMRNGSIAGAFERAEANQQNIMTLALGHHQMVA"
     gene            complement(4680..5342)
                     /locus_tag="VFH91_09230"
     CDS             complement(4680..5342)
                     /locus_tag="VFH91_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6863220.1"
                     /translation="MLELIQPISKRDQVVRAMKDAILSGTIQPGASLVESKIAQQLGA
                     GVPLIREALIELEHQGFVQRTPYKGTTVTTLSPVDVRNIFALRVELEALAIEWARTNT
                     TEADVEYLRELIHKMEDAARALDLPRFYENDLAFHRKIWSLSGNPYLAHTLERLVVPL
                     FAFFLMKTSRQQDSYIESARTHAKIVEAIPTATPTELRDIFKASLVSWKDDMVQLLFS
                     KE"
     gene            complement(5374..>5566)
                     /locus_tag="VFH91_09235"
     CDS             complement(5374..>5566)
                     /locus_tag="VFH91_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863221.1"
                     /translation="ISPRQFWNLVRQNLIEYMSEQPLTGRFKGQASDFLITINHTVLS
                     MTCPEHRSEVLFARRYAKK"
BASE COUNT         1403 a         1527 c         1315 g         1321 t
ORIGIN      
        1 gtgcgccggc ccggcgcact ccaaagttaa aaatcgaatc gatcgatatt ctctttgttg
       61 aaaatgaacg gtgaaccaag gcgcacttca tcgtcaacta catctatttt tccaagtcta
      121 cctgctacca actgatgggc accctgtttt agctgaccgg tgatcaacgc ctgggcagca
      181 taaaccgtca ggtaaccgag gtcggttgtg ttccacagaa caatgctttc cactacgcca
      241 tccttgacat agggtttgtt catgttcggc agagacaatc ccgtcacttt cacatctgtg
      301 cgtcccgctt gcttaactgc ctcagccgct cccggcacag cgggcgctgc aatcgccatt
      361 atgagtttga cgttgggata gactttcaaa actgtttgtg tttcagagaa ggcacggtct
      421 cgatcacctt cgcttggttg gaccgttact aattttaagt cgggatattt ttccgcgaga
      481 cgcgctcgta taaactttat ccattcgttt tgatttgcgg cgctgagtga cgctgtgatc
      541 accgcgaact ctcccttgtt tccaaggatt cgcgcagctt catccgtcag ggtgtagccg
      601 ataccctgcg gcgtcgcctg attgatcaaa aagtcgcggg cgtttcgttc ggcatcagcg
      661 tcccaggtca aaaccttgat gcccttctct cttgccttgc gtaagactgt agaaatgccg
      721 gcctgatttt caacgctgac ggcgatgaca tcaacaccct tggttatcca ggcttcaacg
      781 agttcgttct gttttgcggg atcgaggtcg gttggaccat cccataataa atctattccc
      841 agttccttcg cagcttcttc tgctccctgg cgacagctca caaagtaggg atcaccttta
      901 gcctttggca tcattgccac ctggatacgg tgatttgaat ttgtagccga gttgagtggc
      961 tgttcgcttt gccggccatt ctgaattgag cgtacgagcc accagttact tcctgccacc
     1021 agtaatgcgg caagcagaat aactccactc aaaattgcca cctgcgaatt cttcacatca
     1081 aactcctcag ccccagcgtt gacttgacct tgtccaccaa caaaatttgc gagacggttc
     1141 agtatcaaag taatcaggag taacgctccg gtgagtattc cagccagctc cgcaggctgc
     1201 cggcttaatc gaagcccatt ctgcaggacc acaatcgcga aaagtcccag taccgttccc
     1261 agaactgttc ctcgcccacc aaaaatcgaa gccccgccta aaaccaccgc ggttatcgcc
     1321 atcaattcga atccggtgcc cgcatcagac ttggcctggc ccagatgagc tacataaaca
     1381 acggcagaaa tgctcgcagc aaggcctgaa atcagataga caatgaaaag atttttgccg
     1441 acgggaattc ccgcgtaacg cgcaccttcc ggagagtgtc caattgcata aagtgttcga
     1501 ccaaaggctg tgcgatgaag gcaccaccaa caaagagcga tgccgataat cagaacgact
     1561 agttgggttg gaaccaggtg accgagatag ccctggccca aaaataagaa actggacgaa
     1621 aagcccgagt agttttcgat cccacgagtt aaaccttcag cgacgccgcg aaacagcgag
     1681 aaagtcccca gcgtaacgat cagtggcggc aaaccgatgc gcgcgatcaa cataccgttt
     1741 agtgccccgc ccagcagacc gagaattaat acagccacga tagccagaga catgggccaa
     1801 tgaccgtcgc gccaaagcga gccgagaacg actgcgctta accccatcat cgaaccgact
     1861 gacaagtcga taccacccgt gatgatgaca ggcgttagtg cgagcgcgag cagtccaact
     1921 tcaacactaa gccgtgtaat ctcgaaggcg ttggagcgtg taagaaaatt gtcgccgctc
     1981 aaactaaaga tgccgcattc cagcaccaga accaggatca gtatccactc gcggttagga
     2041 aacgcgcgta agtgccaggg cgctatcagt tttagatccg gtgccaactg cgtgttcgag
     2101 ttcaacgctt aggtctcagt tcgcaagtcg atattgtcgc ctggcggaga attgtgtcat
     2161 cgcaacatca gaaacaacag cagcaataat aatggttccc tgaattgctt tctcccaaaa
     2221 cgggttaatg ccgacaaagg tcaacgctgg accaatcgtt cccagaagca caacgccaat
     2281 aagtgttccc gctacggttc cacgacctcc tgtgatcgca gttccgccta cgacaacagc
     2341 cgcgatcgct tttagctcta acccaattcc cgcattaccg ggaacaatgg aaaaccgaat
     2401 cgcattaagt aaggcggcga gtccggttaa agcacccatg atcacgaagg cattgagctt
     2461 caccaactgc gaatcgatgc ctgcaaggcg agccgcctca gaatctgaac ccactgcata
     2521 gaaaaatctg ccggcaccca gacgacgcaa ggaccaggcg atacttgcaa acacaatcag
     2581 tgctgcaata actaaaagca tttcgccgtg accttgtctg agaccgaacc attgaaagtt
     2641 ctgaggcaaa ctttgaatcc atgcaccctc ggaaccccaa cgcaacgcat ctcgccagat
     2701 caccatcaac gccagtgtgg caacaattga gggaattccg agacgagcca ccagaattcc
     2761 accaaccgca cccatcgcag cgccaatcag tattacggca ggtatcaaca agactaccgg
     2821 cacgccattc tttgctaaca agcccgcagc ataagtacaa actgcaaatt gcgaaccaac
     2881 tgaaatatcg atttcacctg taagtattac gagagtcatt ccgcaggcga caatcaatac
     2941 gctcgcgttg ttgattgcga ggtcgcgtaa gttagccggc tgaaagaacg aaggtgcgag
     3001 tagcgcaacc agcacaagca gtgcaaggta tgagagcgcg actgagaatt cccgcttatg
     3061 cctggttatt aagctggtac ggttcatttg tttgcagcaa tttacgcaac catctgatga
     3121 tggcccaatg cgagcgtcat tatattttgc tggttcgcct ctgccctttc gaacgcccca
     3181 gcgatcgagc cgtttcgcat caccactatg cgatcactca ttccaagaat ctccggtagg
     3241 tccgaagaga tcatgatgat cgctgcacct tgagccgcaa gctctcccat aagagagtga
     3301 atttcggcct tcgctcctac atctattcct tgagttggtt catccaggat taataaagcc
     3361 ggtctcgttt ccagccatcg gctaagcgcc accttctgct ggtttccgcc ggacagcgtc
     3421 gtcactgcag agaacagtgc cggagcctta attgcaaagc gcctcacata tttctcagcg
     3481 atatcgcgct cgcttgctct atcaaacgtt aatccttttc gcagtttgtc cagcgacgcg
     3541 agcgtaacat ttgcgtctat cggcatgtcc agcataacgc cgtgccgcct gcgatcttcg
     3601 ggtagatacg caattccaag ttgaattgca gttgatggct tatcgatgcg cactgtttga
     3661 tcattaatcc gaatctcgcc tcgatccgct ggttcaagtc cgaagattgt gcgggcaagt
     3721 tgagtacgcc ctgcgccaac cagaccagcc agtccaacga tctcacccgc gcgaaccgta
     3781 aggtcaatat tttcgacacc attcaaccga cacgacaggc cctttgtttc caaaaaggct
     3841 ttaccaattc ccacttcgcg tttgggaaag acagcagaga gttcacgccc aaccatcagt
     3901 cgaatcaagt cgtttcgact aacttccgac atcattcgag tatcgactat tacaccgtca
     3961 cgaagcacgg tgactctgtc ggcgaccgcc tcgagctcct caagtcggtg cgatatgtaa
     4021 atgatcgcca ccccatcctt tttcaaggcg tcgattactc gatagagatt ttgcgtctct
     4081 tcttctgcaa gtgaggcggt tggctcgtcc ataataagca gccgggagtt agcccctaca
     4141 gctcgagcaa tttccaccaa ctgctgctca ggcatactca aactctgcac atcgacatca
     4201 gggtcgatcc cagatcctac tctctgaaga agctcaatcg ctcgggctcg tcttgctttc
     4261 caatcaatgc gtctccaggt ccggggtttt tcaattccga gtgagatgtt ttccgttaca
     4321 gtaagttccg ggaacagggc aggttgttga tagatagcgg cgatgccaag tgccttagca
     4381 tgagccggtg aatgatcgaa gatacgctga ccatcgaatt ccactgagcc tctatcgtga
     4441 acaactgcac cggttattgt tttcaccagg gtcgatttgc cggcgccatt ttcaccgaca
     4501 agagcatgaa cctcgccctc acgcaaatcg aaagcgactc ctttcaaggc tgcgacacca
     4561 tcgaatgact tcacgatgtt tttaagtcgc agaatcactc tggagttgga attatgctgg
     4621 tccattcatc ttgcagttag actaattacg agtgaaatga cccgtgaact tttctaccac
     4681 tactctttag aaaacagcag ctgaaccatg tcgtctttcc aactcaccag agaggccttg
     4741 aagatatcgc gcaattcggt tggcgttgcc gtcggaatcg cttcgacaat tttcgcgtgg
     4801 gtacgcgcgc tctcaatata agagtcttgt tgccgactcg tcttcatcag gaaaaatgcg
     4861 aacaatggaa ctacgaggcg ctcaagagta tgagcgagat acggattgcc ggagagactc
     4921 caaatctttc ggtgaaaggc gaggtcattc tcataaaagc gaggaagatc aagtgctctg
     4981 gccgcgtctt ccattttgtg aataagctca cgcagatact cgacatcggc ttcagtggta
     5041 ttggttcgtg cccactctat tgcgagtgcc tcgagttcga cacgaagtgc aaagatattt
     5101 ctgacatcga caggagagag ggtagttacg gttgtgcctt tgtatggtgt tcgttgcacg
     5161 aaaccctggt gctcgagctc gatcaacgcc tcgcgaatta gaggaactcc ggcaccgagc
     5221 tgttgggcaa tcttactctc gacaagagac gctccaggtt gaatcgtgcc cgaaaggatt
     5281 gcatctttca tggcccggac aacctgatct cgtttcgaga tgggctgaat taattcgagc
     5341 atacgtggac gcttctttgt ttggcgatgg gggttatttc tttgcgtagc gtcttgcgaa
     5401 caagacctcc gagcggtgct caggacaggt catgctaagt actgtgtgat tgattgtgat
     5461 taaaaagtct gaggcttgtc ccttaaatct gccagttaaa ggctgctcgc tcatgtattc
     5521 gatcagattc tgccgaacaa gattccaaaa ctggcgcggc gaaatc
//
LOCUS       DASSFP010000410         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_428409, whole genome shotgun sequence.
ACCESSION   DASSFP010000410 DASSFP010000000
VERSION     DASSFP010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428409"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1153)
                     /locus_tag="VFH91_09240"
     CDS             complement(116..1153)
                     /locus_tag="VFH91_09240"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6863222.1"
                     /translation="MSSRKTQSANEAQWQAETDRRRRITMVMLFVFSAFLLIAFAGWF
                     WMTQPLFIRESSSGAQLVEPAQLQQHVNKLSQELGPRDEGHIENLDRVAAYIKEEFQK
                     TNARVSEQPYRVEGKSYRNVIAQFGPTTGRRIVIGAHYDTAGPLPGADDNASGVAGLL
                     ELARLLGKQPPAVLVELVAFTLEEPPYFRTTGMGSSVHAKSLRDQQVEVIAMISLEMI
                     GYFSDSEGSQHFPSAILQAFYPSRGDFISVVGRVGDGLITRRIKAAMLQTASLKVYSI
                     NAPAFIPGIDFSDQLNYWQAGYRAVMVTDTAFYRNLNYHTANDTADKLDYKRMALVVE
                     GVYAAVVALSQ"
     gene            complement(1270..1617)
                     /locus_tag="VFH91_09245"
     CDS             complement(1270..1617)
                     /locus_tag="VFH91_09245"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET6863223.1"
                     /translation="MDIEQIRKFCLSLPYATERVQWGNDLLFCIGNKMFAVAGMDARY
                     PTKFSFKCTPEKFAELVERDGIVPAPYVARYHWVALEDWDAIPQRELKELLKNAYQLV
                     HDKLPKKIRQGLK"
     gene            complement(1607..>2334)
                     /locus_tag="VFH91_09250"
     CDS             complement(1607..>2334)
                     /locus_tag="VFH91_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863224.1"
                     /translation="LNLLSLLAWFARIERGRTRLSLINIGSFPGRPSFRGLGELNPDE
                     LLSLFPTRQAVTANQLRVASDAWIAFCSPDPTQVENFMNQGFSELASLDAALNAHLRR
                     FPSVKNGLGRVEETTLMMVHDGLQTFTDLFLKFGDVAGVYGLGDLQVWSTLVSLSKGR
                     KPLLHLEQTDQAGIKRMQASPRARFTLTDDGHDVLSGTADYARLNDLDIWLGGAHLTN
                     NILWRWNEHTAKLEYQQDNRDGH"
BASE COUNT          593 a          612 c          560 g          569 t
ORIGIN      
        1 tcgaagtacg acagcttgag gtacatggag actgacccga cgctaccgcg aatcggtact
       61 gacttctgct aaaaatgaaa aaatggaaaa tggaaaatga ccagtaagcc ctcccctatt
      121 gactgagcgc caccaccgcc gcgtacactc cttcaacaac caaagccatc cgcttgtaat
      181 caagtttatc agccgtgtca ttcgcagtgt gatagttgag gtttctatag aacgcggtgt
      241 ccgtgaccat caccgctcga taaccagcct gccaatagtt caactgatct gaaaaatcta
      301 tccccggaat gaatgctggc gcattgatcg aatagacctt cagtgaagca gtctgcagca
      361 tggctgcttt gatacgccga gttataaggc cgtcacctac tctgccaacc accgaaatga
      421 agtccccccg tgagggataa aacgcctgaa gaatagcgga cgggaaatgc tggctccctt
      481 cagagtcact aaagtaccca atcatctcga gactgatcat ggctattact tcgacttgct
      541 gatctcttag tgatttggca tgtacagaac ttcccattcc ggtggttcga aaatagggag
      601 gctcctccag ggtaaacgct accaactcaa ccaaaaccgc tggaggttgt ttgcctaaca
      661 gtcgtgccag ctcaagtagt ccggcgacgc cactcgcgtt gtcatcggcg ccaggcaatg
      721 gtccagccgt gtcgtaatga gcgcctatga caatccgcct tccggttgtt ggcccaaact
      781 gggcgatgac gttccggtac gatttaccct cgactctgta cggttgctct gagactctcg
      841 catttgtctt ttgaaattct tctttaatgt aagcagctac gcgatcgaga ttctcgatgt
      901 ggccctcatc ccgcggaccc aattcttgtg agagtttgtt tacgtgctgc tggagctgtg
      961 caggctcaac taattgtgcg cccgaggaag actccctgat aaacaacggt tgcgtcatcc
     1021 aaaaccatcc cgcaaaagcg ataagtagga aagccgaaaa tacaaatagc atcaccatag
     1081 tgatccttcg acgccgatca gtttctgctt gccactgagc ctcgtttgct gactgggttt
     1141 ttcgagagga caacggagtt cctaaagacg aatcattgat caattatgaa ttgatatttg
     1201 agaattacta ttgctgcgaa gggtgagtcc ggacttagca attctcaaat atcaaatcat
     1261 aattgaccat tacttcagtc cttgacgaat tttcttcggt agcttgtcat ggaccagttg
     1321 gtaggcattc ttcaagagtt ctttcaactc acgctgaggt atagcgtccc agtcctccag
     1381 cgcgacccaa tggtaacgcg cgacgtaagg agcgggcaca attccgtcac gttcaaccaa
     1441 ttcagcaaac ttctccggag tgcacttgaa tgaaaacttg gtgggatacc tagcgtccat
     1501 cccggccacg gcaaacatct tattgccgat acaaaagagc aggtcgttac cccactgcac
     1561 ccgttcggta gcataaggta gcgagagaca gaacttgcgg atctgttcaa tgtccatctc
     1621 gattgtcctg ttggtattcg agttttgccg tgtgctcgtt ccagcgccag agaatgttat
     1681 ttgtcagatg tgcaccaccc aaccagatat cgaggtcatt taacctggcg tagtctgcag
     1741 ttcccgaaag aacatcgtga ccatcatccg ttaacgtaaa tctggctcga ggcgacgcct
     1801 gcattcgttt aatgccggcc tggtcggttt gttccagatg caacaaaggc tttcgcccct
     1861 tactaagaga gaccagagta gaccagacct ggagatctcc gagtccatac acgcctgcca
     1921 cgtcgccaaa cttgagaaaa aggtctgtaa aggtttgcag gccgtcgtgg accatcatca
     1981 gcgttgtttc ctctacgcgt cccagaccgt tcttcaccga tgggaaacga cggaggtgtg
     2041 catttaacgc ggcgtcgagt gaggctaatt ccgagaagcc ctggttcatg aagttttcga
     2101 cctgcgttgg atcaggagag caaaaggcga tccatgcatc tgaagcaact cgcaattgat
     2161 tcgcggtgac agcttgcctt gttggaaaca acgacagaag ttcgtccgga tttagttctc
     2221 cgaggccacg aaaactcggc cggcccggaa acgatccgat atttatcaag ctcaagcgag
     2281 tgcgtcctct ttcgatgcgg gcaaaccatg ccagcagact cagaagattc agct
//
LOCUS       DASSFP010000411         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2065694, whole genome shotgun sequence.
ACCESSION   DASSFP010000411 DASSFP010000000
VERSION     DASSFP010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065694"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..669)
                     /gene="kdpC"
                     /locus_tag="VFH91_09255"
     CDS             complement(91..669)
                     /gene="kdpC"
                     /locus_tag="VFH91_09255"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HET6863225.1"
                     /translation="MKNLITAILMTIVTTILLGLVYPLIVTGLAQVIFPDQANGSLIK
                     SSDGTLLGSSLIGQPFSSPGYFRSRPSAAGAVGYDAGASSGSNLGPTNQKLIDRVKAD
                     VEKAKAENPGQPVPIDLVTTSGSGLDPHISPAAAEFQIPRVARERGISADEVRRLINA
                     HTEGRQFGFLGESTVNVLELNLDLDRRYPSKK"
     gene            complement(757..2805)
                     /gene="kdpB"
                     /locus_tag="VFH91_09260"
     CDS             complement(757..2805)
                     /gene="kdpB"
                     /locus_tag="VFH91_09260"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HET6863226.1"
                     /translation="MTTSKKEAIWNSQIVKRAVVDSFKKLNPKKMMGNPVMFVVEVGS
                     VLTSLQLIRGIVAPIPGITNIGFELQITLWLWFTVLFANFAEAMAEGRGKAQADNLRK
                     AKTETIAHRVVSGSQTEDVPAPQLRKDDLVIVSAGEFIPGDGEIIEGVASVDESAITG
                     ESAPVIREAGGDRSAVTGGTRVLSDQIKIRITSNPGETFIDRMIALVEGAERQKTPNE
                     IALNILLAGLTIIFLLAVVTLQPFAIYSSAPQTIFVLVSLLVCLIPTTIGGLLSAIGI
                     AGMDRLIQHNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQATAFIPLPGVDETELA
                     EAAQLSSLADETPEGRSIVVLAKEKYGLRGRELAPHEATFVPFTAQTRMSGVNFDGRE
                     VRKGAADAILKYLGSSSNNHVADLNTIVERISREGGTPLVVAEDRLPLGVIQLKDIIK
                     GGMIERFNQMRQMGIRTVMITGDNPLTAATIAREAGVDDFLAQATPEDKMALIRKEQA
                     GGKLVAMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEIVEIGK
                     QLLMTRGALTTFSIANDVAKYFAIIPAMFAGAFPVLNALNIMGLRTPQSAILSAVIFN
                     ALIIITLIPLALRGVKYRPMSAASLLRRNLLIYGFGGIIVPFIGIKIIDVIIHAIGLA
                     "
     gene            complement(2947..>4093)
                     /gene="kdpA"
                     /locus_tag="VFH91_09265"
     CDS             complement(2947..>4093)
                     /gene="kdpA"
                     /locus_tag="VFH91_09265"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HET6863227.1"
                     /translation="GNVLTNPDGTNQKETKVITEQTIAQGPVASQEFIKELGTNGGGF
                     FNVNSAHPFENPTPFTNFIELLAIFAIGGGLTYTLGRMTGSPKHGWAVLAAMVVLCLA
                     GVLTSYWAESRGNPILTGIGANQQTSAMQTGGNMEGKEVRFGIASSALFATVTTDASC
                     GAVNSMHDSFTPLGGLVPLVNIMLGEVIFGGVGSGLYGILIFVILSVFIAGLMVGRTP
                     EYLGKKIESYDVKMSMLYVLIFPLIILVFAGISVLAPGFGTSQLNNAGPHGLSEILYT
                     FTSATGNNGSAFAGISANTYWYNTTIGLAMLIGRFLMIVPMLAVSGNLASKKLVPPSL
                     GTFPVTTPLFTVLLISVILIVGALTFFPALSLGPIVEHLLMEAGKTF"
BASE COUNT          982 a         1082 c         1039 g          990 t
ORIGIN      
        1 caaagattcg ggtgaacgac ggttaccgtt atcgctcatg ggcaatttat ataggaccga
       61 tgcgacctat aggacctata gctttgaacc ctactttttc gacggatacc tcctgtctaa
      121 atccaaattg agttccaaca cgttcacagt tgattcgccc aaaaaaccga actgtctacc
      181 ttcggtatgg gcatttatga gtcttcgcac ttcgtccgca ctgatgccgc gctctcgagc
      241 aacacgaggg atttgaaact cagctgcggc aggagagata tgcggatcaa gtcctgatcc
      301 ggatgtcgtt actaaatcga taggcacagg ttgaccggga ttctcggctt tcgctttctc
      361 aacatcagct ttgacccgat caattagttt ttgattagtt ggcccgaggt tcgagcccga
      421 ggacgcgccg gcgtcataac cgactgcacc cgcggcggac gggcgagaac gaaagtagcc
      481 aggagaagaa aaaggctggc caattaaact tgagccaagc agtgtgccat cggagctttt
      541 gatcaatgag ccgttggcct ggtccgggaa aattacctgg gccaagcccg tcacgatcag
      601 cggatatacg agacctagca agatagtcgt aacaatggtc atcaggattg ctgttattag
      661 gttcttcatt gtcagttgtc cgttgtcagt ggtccgttgt gcccttggtc gatccgtgtc
      721 agccactgac cactgacaac ggaccactga cagtccttaa gccaaaccga tcgcatgaat
      781 aattacgtcg attatcttga tgccgataaa cggcacgatg attcccccga agccatagat
      841 aagtagattg cgacgcagca acgatgctgc actcatcggc cgatatttca ctcctcttag
      901 tgcaagcgga ataagagtga tgatgatcaa tgcgttgaag atcacggcgg agagaattgc
      961 ggactgcggc gttcgcagac ccatgatgtt cagcgcgtta agaactggaa acgcgccagc
     1021 aaacattgct ggaatgatgg caaagtattt cgcgacgtca tttgcgattg agaacgtcgt
     1081 caaagcaccg cgtgtcatta atagttgctt tccgatttcg acaatctcga tcagtttcgt
     1141 cgggttggag tcgagatcga ccatgttccc tgcttctttg gccgcttgag ttccagtgtt
     1201 catcgccacg ccaacatcgg cctgggccaa agctggagcg tcgttagtac cgtcaccagt
     1261 catcgcaacc aatttgccgc cggcctgttc ctttctgata agcgccatct tgtcttctgg
     1321 tgtggcttga gcaagaaagt catcgacacc tgcttcacga gcgatggtcg cggcagtaag
     1381 aggattgtcg ccggtgatca tcaccgtgcg gatgcccatc tgacgcatct gattgaaacg
     1441 ttcaatcatt ccacccttga tgatgtcttt aagttgaatc acaccaagag gcagacggtc
     1501 ctcggccacc actaaagggg ttcctccctc acgtgaaatt cgttcgacaa tggtgttcag
     1561 atcagcaacg tgattatttg aggaggaccc aagatatttc agaatcgcat cggcggcacc
     1621 tttgcgcact tcacgaccat caaagttcac accggacatt cgcgtttgag cggtaaaagg
     1681 aacgaaggtc gcttcatgag gagctaactc tcttccacgc aggccatatt tctcttttgc
     1741 cagtactacg atcgatcgac cttccggcgt ttcatccgca agtgaggaca attgagcggc
     1801 ttccgctaac tccgtttcat ctactccggg tagcggaatg aacgcggtcg cctggcgatt
     1861 acccagagtg attgttcctg tcttatccaa taacagcgta ttcacgtcgc cagctgcttc
     1921 caccgcgcga cctgacatgg ccagcacgtt atgctgaatc agccgatcca ttcctgcgat
     1981 accgattgca gaaagcaatc cgccaattgt tgtaggaatc aaacagacaa gcagcgagac
     2041 gagtacaaag attgtctgcg gtgcgcttga gtagattgcg aatggctgca acgtgacgac
     2101 tgccaggaga aaaatgattg tcagtccggc aagcaaaatg ttgagcgcga tttcgttcgg
     2161 agtcttctga cgctcagcac cttcaacgag tgcgatcatg cgatcgataa acgtctcgcc
     2221 tggattggaa gtgattctga ttttgatctg gtccgaaagg actcttgtgc cacccgtgac
     2281 tgcagagcgg tcaccacccg cttcgcgaat aactggcgcg ctttcgcctg tgattgccga
     2341 ctcatcaacg gaggcgacgc cttcaatgat ctcgccatcg ccaggtatga actctcctgc
     2401 cgacacaatg accagatcat ctttgcgcag ttgtggtgcc ggcacatctt cagtctgtga
     2461 acctgaaacc actcggtgag caatcgtttc agtcttcgcc tttcgcaggt tatcagcttg
     2521 tgctttaccg cggccctcgg ccattgcttc ggcgaagttg gcaaagagca cggtaaacca
     2581 cagccacaac gtaatctgca attcaaaacc gatattcgtt atgcctggaa tcggcgcgac
     2641 gattccgcga atcaactgga ggcttgtcag cacgcttccg acctccacaa cgaacatgac
     2701 gggattaccc atcatcttct tcggattcaa tttcttgaac gaatcgacga ctgctcgctt
     2761 aacgatctgc gagttccaaa ttgcttcttt cttagatgta gtcatgggtg tattggtctt
     2821 cggttgggtc ttgggttggg tctttggtct tgggccttgg gtctttcatc ctcttattcg
     2881 ttcgttctgg ctttagcatt caaatcgcga acaaacgcca aagaccaaag tccaaagacc
     2941 tccctctcag aatgtcttcc ctgcctccat caaaaggtgt tcgacgattg gtccaagact
     3001 caacgccggg aaaaacgtca acgcgccaac gatcagaatt acgcttatca atagaaccgt
     3061 gaacagcggg gtcgttactg gaaacgttcc taatgaaggc ggcaccagct tcttcgaggc
     3121 gagattccca gagactgcaa gcatcgggac aatcatcagg aagcggccaa tcagcattgc
     3181 caggccaatt gtggtgttgt accagtaggt gttcgccgaa ataccggcga aggccgagcc
     3241 gttgtttcca gttgcagaag taaacgtgta gagaatctct gatagaccat gtggaccagc
     3301 gttgttcaat tgcgatgtgc cgaagcccgg ggccaggacg gagatgccgg caaaaacgag
     3361 aatgatgagc gggaagatca acacatacag catcgacatc ttcacgtcgt acgattcgat
     3421 cttcttgcca aggtactccg gcgtgcgtcc aaccattaga cctgcaatga agacagacag
     3481 gatcacgaag atcaggatgc cgtagagtcc actaccgacg ccgccaaaaa tgacctcgcc
     3541 cagcatgatg tttaccagcg ggaccaatcc accgaggggc gtgaagctgt cgtgcattga
     3601 gttgaccgcg ccgcaactcg catccgtcgt gaccgtggcg aacagcgcgc tgctcgctat
     3661 tccgaagcga acttccttgc cttccatgtt gccgcccgtt tgcattgcac tggtctgctg
     3721 gttggctccg ataccggtaa gaatcggatt accgcgtgat tcggcccaat aagaggtcaa
     3781 gacacctgcg aggcatagca ctaccatcgc ggcgagtacg gcccacccat gcttcggcga
     3841 tccagtcatg cgcccgaggg tgtaagttag accaccacca atcgcgaaga ttgcgagtag
     3901 ctcaatgaag ttcgtaaagg gtgtcggatt ttcgaatgga tgagcggagt taacattgaa
     3961 gaaaccgccg ccattagtgc ccaattcctt gataaattcc tgcgatgcga ccggaccctg
     4021 cgcgatggtt tgttctgtaa ttaccttcgt ttctttttgg ttggtcccat caggatttgt
     4081 caggacgttt ccg
//
LOCUS       DASSFP010000412         6788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1154457, whole genome shotgun sequence.
ACCESSION   DASSFP010000412 DASSFP010000000
VERSION     DASSFP010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154457"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFH91_09270"
     CDS             complement(<1..516)
                     /locus_tag="VFH91_09270"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HET6863228.1"
                     /translation="MTTKPKKHLFQAHPWHGVTPGSRVPQLVNAYIEIVPTDPVKYEL
                     DKASGHLRVDRPQRFSSMCPTLYGFIPQSYCDVEVAELTAQRTGRQGIEGDKDPMDIC
                     VLSEKTFAHGSFFLNARPIGGLRMIDGQQADDKIIAILEADLAYDYIGDIGDVPTGLV
                     DRLKHYFLSYKQ"
     gene            598..1692
                     /locus_tag="VFH91_09275"
     CDS             598..1692
                     /locus_tag="VFH91_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863229.1"
                     /translation="MAASVQATAKPIASALSLRWIISARDDLLWFIGSVASSYILLLL
                     YISGVLPLVVMAALWAILIDAPHVFGTFSRTYFDRTEWQNRPRLLWGSLLFFLVGPVM
                     VLSGAAFVFFFVAALWAYYHLVKQHYGFMVLYKKKNGDLAAIDNVLDRLLLLFAFNYP
                     FVEFIARDPEAMARVPASLRGGVNGLARILFVATMLLLVVWIVRQFQRLLTGEPLNLP
                     KYLLLAAAIPMHWIVLLTPMPHKPIAIVAILTIYHNLQYHRLIWFHNQKYRGSSIEVR
                     KKFGAAETISRRLAYYVGFGIVFGIIYQGPRQILGYLNLQQGESTQQSLLIQLGISFL
                     WGYAFIHYYLDSKIWRVRRDPSVGEALRMS"
     gene            complement(1727..3973)
                     /locus_tag="VFH91_09280"
     CDS             complement(1727..3973)
                     /locus_tag="VFH91_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing transglutaminase
                     family protein"
                     /protein_id="HET6863230.1"
                     /translation="MRPAPSWVRTVVASTGEETSLPPGTSSSQLLSDEQTKVAENGSV
                     ERYFHYVTRVDGASGLDDLSQLRFYFEPSYQQLVIHYLRIQRGNSSIDILKPSEIKIV
                     QQEEELDQQLYNGTLASVIFVNDLRIGDSVDYAYSINGENPVLGGRFTDTLYMADTEP
                     IQEHVIRLIVPSDRSLAIKNNNTKLTFTSQSIGQDTEYLWYDKKIPAVSKDDYTPVWF
                     DPYPSISVSEFRNWSDVVQWALPFYQQSPLRNRELIAKVEQWKQASSSSEERIIAALR
                     FVQDEIRYLGIELGRYSHQPTSPDQVFARRFGDCKDKSLLLSSILAAMDIDAVPVLIN
                     TRARETLDSWQPSPFAFDHVIVRATLNGKTYWFDPTISYQRGTLRTYYDPPYERALVL
                     RPGEQALSQIPRPAVGSRSISIVESYNGGNGQPVTLNVATTYRGADADEVRYGLATES
                     LADLSKEYLNYYADSMPSISANGLPVVKDDAITNTIEVTENYLIDDFWKNKAHSFLAD
                     KVYVELDKRGLSQRTMPLKLKYPLSVDQTILVNLGSGYDFPIGNERFSDDALSFQALA
                     YKNGTQFVLHCSLITFADSVPVDKMQNHLQVLSEARGLAGFDLAQGSARPGFSAGLTI
                     PLLLFLAFVLVVGGFVVVRLLRDNSKGRAKDFAEKLKVNPGTTAEAALEVSSNEQLES
                     MSRQFQCRCGGNAYDPAKPPLQERFSYDGEKLVGVRFVCAACGLTSDLYFRFRREPTT
                     EGLPSLST"
     gene            complement(4257..5687)
                     /locus_tag="VFH91_09285"
     CDS             complement(4257..5687)
                     /locus_tag="VFH91_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET6863231.1"
                     /translation="MEDKEIKELEKAVEQIWEVAQRFGLDPYPTNFEIVPAPVMYEVG
                     SYALPGRYSHWTFGKAYHRMKTMYDFGLSKIYEVVINTNPSYGFLLETNSPIQNKMVI
                     AHVLGHVDFFKNNLYFSKTNRRMVESVSTHAQRMMEYEFKHGRKTVERFLDSVLAIEE
                     HIDPYFFIKRNLTPAEEKRRAEQRPKDGRYDDLWLLDKETRDARLEKFPQSNDREHVP
                     QERLPEKDLLFYIMKNSPSLVGWQRDAIAMVHEEMEYFVPQMQTKIMNEGWASIWHAR
                     IMRELDLSDNDHIEFANLHSGVVSPQKGQLNPYYLGYKIYEDIERRWNEPSDEERERF
                     GRIGNQGREKIFEVRELENDVSFLRNYLTEELCEELDLFVFELVEEEEWTITEKRWER
                     VRDQLVANMTNFGFPYLEVQDGDYNGNRELYLKHRYEGTELDMRYARKALEHVYTLWG
                     RPVHLETVIDDESSVLHFNGDEHAED"
     gene            complement(5738..>6788)
                     /gene="yhbH"
                     /locus_tag="VFH91_09290"
     CDS             complement(5738..>6788)
                     /gene="yhbH"
                     /locus_tag="VFH91_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HET6863232.1"
                     /translation="NEAIILSDGKRTVKVPIKALDEYKFRFNYQKGKHVGQGDGKTRV
                     GDVIGRESRPGSGSGSGQAGQDSGVDYYEAEVNIDEIAALIFEDLQLPYLQEKAKKSV
                     QSRKTQFNEIRRTGVIANLDKRRMILENIRRNAREGNPRFGGIKKEDLRFKTWEEVMR
                     YESNAVVIAMIDVSGSMGEFKKYIARSFYFWMVRFLRTKYDNVDIVFISHHTEAKEVT
                     EEQFFTQGESGGTVVSSAYQLALEIIKQRYRPADWNIYPFHFSDGDNYYSDNEEAVRL
                     ADELITTCNLFGYGEIGEEGSSNYRRSSGALLSIFGDRLKNKDRFVGVRIDDKEDVYP
                     ALKQFFGKRGVETPA"
BASE COUNT         1564 a         1701 c         1675 g         1848 t
ORIGIN      
        1 ttgtttgtag ctcaaaaagt agtgtttcaa acggtcgact aaaccggttg gcacgtcgcc
       61 aatgtcacca atatagtcat acgccaggtc ggcctccaga attgcgatga tcttgtcgtc
      121 agcttgttgg ccatcgatca tcctcaaacc gccgattggc cgcgcattca aaaagaagct
      181 gccatgcgcg aaagtcttct ccgaaaggac gcaaatatcc atcggatctt tgtcaccttc
      241 gattccctgg cgccccgtac gttgcgcggt gagctccgct acttcaacgt cgcagtagga
      301 ttgaggaatg aaaccgtaga gagtgggaca cattgatgaa aagcgttgcg gccggtcgac
      361 tcgtaagtgg ccagatgctt tatcaagctc atatttgacc ggatccgtag gaacgatttc
      421 aatgtaggca ttgaccaact gcggaactct agatcccggt gtcacgccat gccatggatg
      481 cgcttgaaat aggtgttttt ttggtttggt ggtcatggga aagtgcaaaa cccgtagcgt
      541 agctacaagc accaacccgt cgatgataga atgcgcccac tctgattaca aatactcatg
      601 gcagccagcg ttcaagctac agcgaaacca attgcgagcg ctctatcatt acgttggatt
      661 attagcgctc gcgacgatct actctggttt attggctctg tagcttccag ctatatctta
      721 ttactgcttt acatctctgg tgttttgccg ctagtcgtaa tggccgcgct ctgggccatc
      781 ctgattgatg cgcctcatgt gttcggtaca ttttctcgta cctacttcga tcgcaccgag
      841 tggcaaaaca gacctcgact gctgtggggt tcattgcttt tctttctggt tggtccagtg
      901 atggtgttat caggcgccgc gttcgttttc ttcttcgttg ccgctttatg ggcctactat
      961 cacctcgtga agcaacatta cggcttcatg gttttgtata agaagaaaaa cggcgacctt
     1021 gctgcgatag ataatgttct ggatcgactg ttattgctgt ttgctttcaa ctatccgttc
     1081 gttgaattca tcgcgcgcga tcctgaggcg atggcgcgag tcccagccag tctacggggt
     1141 ggagtcaacg gtcttgcgag gattctcttc gtcgccacca tgcttctttt ggttgtttgg
     1201 attgttcgac agttccagcg tttgctcaca ggtgaaccgc tgaatcttcc caagtatctt
     1261 ttgctggctg cagcgattcc gatgcattgg atagtgttgt tgacaccaat gccgcacaag
     1321 ccgatagcaa tcgtagctat tctaacgatc taccataacc tccaatatca ccgtttgatc
     1381 tggtttcaca atcagaagta caggggttct tcaattgaag ttagaaagaa atttggcgcc
     1441 gccgaaacaa ttagccggcg tctcgcctac tatgttggtt ttgggattgt ctttggaatt
     1501 atctaccagg gaccgcgaca aattttaggt tatctaaact tgcagcaagg cgagtccact
     1561 cagcagtcgc tactgattca gctgggtatt tcttttctat ggggttatgc cttcattcac
     1621 tactacctgg attcaaaaat ctggcgggtg cgtcgcgatc catccgtcgg agaggcttta
     1681 cgaatgagct aggcttcggg agtgccttct actttgatct ctcacattaa gtactcaagc
     1741 ttggcagacc ctcagttgtt ggttcgcgcc gaaacctgaa gtacagatcg ctcgtcagac
     1801 cgcacgccgc acatacaaat cgcactccaa ccagtttttc accgtcgtaa ctgaatcgtt
     1861 cctgtagcgg cggttttgct ggatcgtaag catttcctcc gcaccggcat tggaattggc
     1921 ggctcatcga ctctaattgc tcgtttgaac tcacctcaag cgccgcttca gcagtagtgc
     1981 caggattcac ctttagcttt tcagcaaaat ctttggctct gccttttgaa ttgtcacgta
     2041 gcaaacgaac aacaacgaat cctccgacga ccaacacaaa cgccaggaag agtaagagag
     2101 gaattgttaa gcctgcactg aagcctggtc gagccgaacc ctgggccaga tcaaacccgg
     2161 ctaatcctcg tgcctcactc agaacctgca aatgattctg catcttgtcg accggtaccg
     2221 agtcggcaaa cgttattagt gagcagtgaa gaacgaactg agtaccattc ttgtacgcta
     2281 gggcctggaa gctaagcgcg tcgtcgctga accgctcgtt cccaattgga aaatcatagc
     2341 ccgaccccag attgacaaga atcgtttggt cgacgctcag tggatactta agtttcaaag
     2401 gcatggtccg ctgtgacaag ccacgcttgt ccaactcaac atagacttta tccgcgagga
     2461 agctgtgggc cttatttttc cagaaatcat caatcaaata gttctcggtg acttcgatcg
     2521 tgttcgttat cgcatcgtct tttactactg gtaagccgtt cgcgcttatc gagggcatcg
     2581 agtcggcgta gtaattcaaa tactccttac ttaggtctgc tagcgattcg gtagcaaggc
     2641 cataacgcac ttcatctgca tcggcgcctc gataggtggt tgctacgttc agagtcacgg
     2701 gctgtccatt gccaccgtta tacgactcga cgatgctgat agagcgagat ccgactgccg
     2761 gccgcggtat ttgcgaaagc gcttgttcac cgggacgaag aaccagagct cgttcatatg
     2821 gtggatcgta gtaagttcgt agagtgcctc gttggtaact aatggttgga tcgaaccagt
     2881 aagttttgcc gttgagagtc gcgcgtacaa tgacgtggtc aaaagcgaat ggagatggtt
     2941 gccatgaatc gagtgtttct ctggccctcg tatttatcaa cacgggaaca gcatcgatat
     3001 ccattgctgc aaggatggaa ctcaacagta gcgatttatc tttgcaatct ccgaagcgcc
     3061 gcgcgaatac ctggtctgga gaagttggtt gatgtgagta ccttccaagc tcaatgccga
     3121 gataacgaat ttcgtcctga acgaagcgga gagcggcgat gatcctttct tcggatgaac
     3181 tagaggcttg tttccattgc tcgacttttg cgattagctc tcgattgcgc aaaggggact
     3241 gttgatagaa tggcagcgcc cattgaacta cgtccgacca attgcgaaat tcactcacag
     3301 atatacttgg ataagggtca aaccagaccg gcgtgtagtc gtctttgctg accgccggaa
     3361 tcttcttgtc gtaccagagg tattccgtat cttgaccgat ggactggctc gtaaacgtta
     3421 gtttggtgtt gttgttcttg attgccagcg aacgatcgga tgggactatg agccgtatca
     3481 catgttcctg gatcggttcc gtgtctgcca tgtatagcgt gtccgtaaag cggcctccaa
     3541 gtactgggtt ctcaccattg atcgagtatg cgtaatcaac cgaatcaccg attcgaaggt
     3601 cgttgacgaa aatgacagaa gcgagagttc cgttatatag ctgctggtcc aactcctctt
     3661 cttgctgaac tatttttatt tccgatggtt taagtatgtc gattgaggag ttgcctcgct
     3721 ggatcctgag gtaatgaatc accagttgct ggtacgacgg ctcgaagtaa aaccgcaatt
     3781 gcgacaagtc gtccaaacct gaggcgccat ctacacgggt tacataatga aaatagcgct
     3841 cgactgaacc attctcagcc accttcgttt gctcatcgct gagtagctga gatgagctgg
     3901 tgcccggagg tagagaagtc tcctcgcccg tggatgcgac aactgtgcga acccatgaag
     3961 gcgctggtct cacggaaaac gaactcttgc ctagcgagac tgtggaactg gctagagtga
     4021 gagcagtgag aaaaagaaga cgaaatagcg atgggtaact cgttgaaagc acgagatgaa
     4081 gcctcggaat cggattagca ggtaaatagt cgtgaagccg gtcagggccg gatgttaact
     4141 atgtgcccgg ttgagaataa tacgaatgga ctgaaagggt ctacagatct gttctccatt
     4201 cccttcagtg cctgattggt gtgatggggt gcgcccacgg agggacgccc tacgaatcaa
     4261 tcttcggcgt gctcgtcgcc gttgaaatga agaacggagg actcatcgtc gataactgtt
     4321 tcaaggtgaa cgggacggcc ccagagcgtg taaacatgtt ccagcgcttt gcgggcgtaa
     4381 cgcatgtcca gctctgtccc ttcgtaacga tgtttcaaat atagctcacg gttcccgttg
     4441 taatcaccgt cctggacttc gagataaggg aatccaaagt tcgtcatgtt cgcgactaac
     4501 tggtcgcgaa ctctctccca gcgtttttca gtgatcgtcc actcttcttc ttcgactaat
     4561 tcaaatacaa acaggtcgag ctcttcgcat aattcctctg tcaggtaatt gcgaagaaaa
     4621 gagacatcat tctccagttc ccgtacttca aagatcttct cgcgtccctg atttccgatg
     4681 cgaccaaagc gctcgcgctc ctcatctgag ggctcgttcc agcgccgctc gatatcttcg
     4741 tagattttgt agcccaggta gtaagggttg agttgtcctt tttgaggtga gaccactccg
     4801 gaatgcaaat tcgcgaattc gatgtgatcg ttatccgata ggtcgagttc ccgcatgatt
     4861 cgagcgtgcc agatgctggc ccaaccttca ttcataatct tggtttgcat ctgcgggaca
     4921 aagtactcca tctcctcgtg caccatcgca atcgcatcgc gttgccaacc aacgaggctc
     4981 ggagaatttt tcatgatgta gaagagaagg tctttctccg gcagtcgttc ttgtggtacg
     5041 tgttcgcgat cattcgactg tgggaacttt tcgagccgcg cgtcacgtgt ctctttatct
     5101 aacaaccaga gatcatcgta acgtccgtcc tttggccgtt gctccgcgcg gcgtttttct
     5161 tctgccggtg tcagatttcg tttgatgaag aaataaggat caatgtgttc ctcgatggcc
     5221 agaaccgaat ccaggaatcg ttccactgtc ttacgtccgt gcttaaactc gtactccatc
     5281 attcgctggg cgtgagtcga gacactctca accatccggc gattcgtctt tgagaagtac
     5341 agattgttct tgaagaaatc gacgtggccc agaacgtggg ctatcaccat cttgttctga
     5401 ataggactat tggtttcaag caggaagcca tacgaaggat tggtattgat tacgacttcg
     5461 taaatctttg aaagaccaaa gtcgtacatc gtcttcatcc ggtggtaggc cttgccgaat
     5521 gtccagtgcg aatagcgacc cggaagcgcg taggagccca cttcgtacat taccggtgct
     5581 ggaactattt cgaaattggt tggataggga tccaggccga accgttgagc cacttcccat
     5641 atttgctcga cggccttttc cagttctttg atttctttat cttccatata ttggacctat
     5701 aggagttata taggtcatat aaccctcgac tcacggacta tgccggcgtc tcaactcctc
     5761 gctttccgaa gaactgtttg agcgccggat agacgtcttc tttgtcatca attcgcacac
     5821 ccacaaatcg atctttgttc ttcagccgat cgccaaaaat tgagagcagt gcaccggaag
     5881 atcgtctgta atttgaagat ccttcttcgc cgatctcgcc atagccaaat agattacagg
     5941 tggtgatcaa ttcatctgcc agtcttaccg cctcctcatt atcggagtag tagttgtcgc
     6001 catcggaaaa gtgaaaggga tagatgttcc agtctgccgg gcgataccgt tgcttaatta
     6061 tctcgagtgc cagttgataa gcagaagata caactgtccc gcctgactcg ccctgggtga
     6121 aaaactgttc ttcggtaact tctttcgctt ccgtgtgatg cgagatgaaa actatatcga
     6181 cgttgtcgta cttggtcctg aggaatcgga ccatccagaa gtaaaaactt cgcgcaatat
     6241 acttcttgaa ctcgcccatt gagccggaca catcgatcat ggcgattacc actgcgttcg
     6301 actcgtaacg cattacctct tcccacgttt tgaaacgcag gtcttctttc ttaataccgc
     6361 cgaatcgtgg attaccttct cgcgcgttgc gtcgaatatt ctcgaggatc attcgtcgct
     6421 tatcgagatt ggctatcact ccggtgcgcc gaatctcatt gaactgggtc ttgcgcgatt
     6481 gaacagattt ctttgccttt tcttgcaggt aaggaagttg caggtcttca aagatcagtg
     6541 cggcgatctc gtcgatattg acttcggcct cgtaatagtc gaccccactg tcctgaccgg
     6601 cttgaccaga tccgctgcct gagccaggcc ggctctcgcg accgatcacg tcgccgacac
     6661 gagtcttccc gtcgccctgt ccgacgtgct tacctttttg ataattgaac cggaacttgt
     6721 attcgtcgag agccttaatg gggactttga cggtgcgctt gccgtcggag aggatgatgg
     6781 cttcgttc
//
LOCUS       DASSFP010000413         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2211169, whole genome shotgun sequence.
ACCESSION   DASSFP010000413 DASSFP010000000
VERSION     DASSFP010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211169"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VFH91_09295"
     CDS             <1..1132
                     /locus_tag="VFH91_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6863233.1"
                     /translation="VKAFGAKELMRLAEPDGKIGHAELQLGDSILKISDEYPEYGAVS
                     PQTLGGSPVKLHLQVEDVDEFAQRAVAFGATISRPIEDQFYGDRAGQLSDPFGYTWIV
                     ASHIKDVSVEEMQEEFDSFNKQKTSKKRREGFHSVTPYLTVKEAPELVDFVKTAFGAT
                     ETFRTIGSAGGLHCEVRIGDSMVMIGGGPEISEWPTAIHLYIPDVDDAYKRAIEAGAI
                     SLFEPADQPYGERSGAVQDRMGNRWYIATTTVPLSEIDRDLHTVTVYFHPLKAQQLID
                     FLTNAFGAEEIMRSDSGDGVIYHAKVRIGDSVVEMGDARDVSQPMPTAIYLYVDDVDA
                     TYEQALKAGAVSVVPPADQPYGDRNAWVKDPFENIWYIAAPV"
     gene            complement(1264..1911)
                     /locus_tag="VFH91_09300"
     CDS             complement(1264..1911)
                     /locus_tag="VFH91_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6863234.1"
                     /translation="MTFPKTDRTTLKRLPKRGHYEHDTVNSILDEGFICHVGFVFDGK
                     PVVIPTGYARMDDRLYIHGSQASRMLRTLSEGIDVCVTVTLVDGLVLARSAFHHSINY
                     RSVVVFGRAVRIDDPVEKDAALFAFSEQVIRGRWEDVREPTENELKQTSVLCLPLTEA
                     SAKIRTGPPIDDEEDYALSIWAGVLPLQVIPGEPIKDPRLPDGIEVPQYVKTYRR"
     gene            2073..>2563
                     /locus_tag="VFH91_09305"
     CDS             2073..>2563
                     /locus_tag="VFH91_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863235.1"
                     /translation="MHFRRSLAAICLSILCCVGNAFAQDGINKSPIETAFAKLEWRSI
                     GPANMGGRVADIEGVPGDPNIVYVATGSGGLWKTTNGGTTWKPIFEREGTISIGDIAL
                     APGNPDVVWVGTGESNVRNSVSFGDGVYKSTDGGKSWKNTGLKETEHISAIAINPQNP
                     DMV"
BASE COUNT          664 a          612 c          658 g          629 t
ORIGIN      
        1 cgttaaggcg ttcggagcta aagaactgat gcgtctggct gagccggacg gaaaaatcgg
       61 acacgctgaa cttcaattag gtgattcgat cctgaagatt tcagacgaat atcctgagta
      121 cggcgcagtc agtccacaaa ctctaggcgg ctcgcctgta aaacttcatc ttcaggtgga
      181 agacgttgac gaatttgcgc aaagagcagt cgcttttggc gcgacgatca gccggccgat
      241 tgaagatcaa ttctatggtg atagagctgg ccaactaagt gatccattcg gttatacctg
      301 gatcgtagcg agccatatta aggacgtttc ggttgaggag atgcaggaag aattcgactc
      361 gttcaataaa cagaaaacgt caaagaaaag acgtgaaggg ttccacagcg tgacacctta
      421 ccttactgtg aaagaagcgc cagagctcgt cgatttcgtg aaaacagcat ttggtgcgac
      481 tgagaccttt agaacgattg gatcagcagg aggtcttcac tgcgaggtaa gaattggaga
      541 ctcgatggtg atgattggtg gtggacctga aatttcagag tggccgaccg caatccatct
      601 ttatattccc gacgtcgacg acgcatataa gcgtgcaatt gaggctggtg caatctcgct
      661 ttttgaaccg gccgatcagc cttacggtga aaggtcagga gccgttcaag atcgaatggg
      721 caaccgatgg tacatcgcga ccacaaccgt accacttagt gaaatagata gagatctgca
      781 tacggtcact gtttactttc atcctctcaa ggctcagcaa ctgatcgact tcttaacaaa
      841 tgcttttggc gctgaggaga tcatgcgttc agactctgga gatggcgtca tctatcacgc
      901 aaaagtacga attggcgatt cagtggtaga aatgggtgac gcccgagatg tttcgcaacc
      961 gatgccaacc gccatctatc tatatgtcga tgacgttgat gctacatacg agcaggcgct
     1021 gaaagccgga gcggtatctg tcgtgcctcc ggctgatcaa ccctatgggg atcgcaacgc
     1081 atgggttaaa gatccattcg agaatatctg gtacatagcc gccccggtct aactttggag
     1141 tgcgccggct ttggagtgcg ccggctcggc ggcgctttgt tacaccgaaa tcatccgaac
     1201 tcgcaatgta accaaagcgc cgccactcat gcgcactcca agcggagtaa atcaaacaca
     1261 ccctcatcga cggtaggttt ttacgtattg cgggacttcg attccatcgg gtaagcgtgg
     1321 atcttttatc ggttcgcctg gaatcacttg taggggcaaa accccagccc agatcgacaa
     1381 agcataatct tcttcatcat caatcggtgg accagttcgg atttttgctg aagcctcagt
     1441 taacggcaaa cacaaaacac tcgtttgctt caactcattc tctgtgggtt cgcgaacatc
     1501 ttcccaccgt ccgcgtatca cttgttccga aaaagcgaac aacgccgcat ctttctccac
     1561 tggatcatca attcgtacgg cacgaccaaa aacaactacc gagcggtaat taatcgaatg
     1621 atgaaaagct gaccgggcga gcactagtcc gtcaaccagc gttaccgtca cacacacatc
     1681 gatgccttca gaaagggttc gcaacattcg gcttgcttgg gagccgtgga tgtagagtct
     1741 gtcgtccatg cgagcgtagc cagtaggtat gaccaccggt ttgccgtcaa aaacaaaacc
     1801 gacgtgacaa ataaagccct catccagaat ggagttgaca gtatcgtgtt cgtagtgccc
     1861 acgttttggt aaacgcttga gcgtagtccg atcagttttg ggaaatgtca tgcgttggat
     1921 agtaggattt tttcgagctc tgcgacaact gtagagaata gggcctatcg gtagtgtagg
     1981 tcccattttc atattgacag ttattgatag ttcctgtttt ctttagtggt ttttccagac
     2041 cgtctaacac ctacagttct caggagaaca agatgcattt tcgtagatcc ctggccgcta
     2101 tctgtctgtc tattctttgt tgcgttggga acgcattcgc tcaggatggg attaataagt
     2161 cgccgattga aactgccttc gcgaaactcg aatggcgaag cattggtcct gcaaacatgg
     2221 gcgggcgagt cgctgatatc gaaggcgttc cgggcgatcc aaacattgtc tatgtagcca
     2281 cgggctcggg tggactctgg aaaactacga atggcggaac tacctggaaa ccgattttcg
     2341 agcgagaagg gaccatttca attggcgaca tcgctctagc tcctggaaac cccgatgttg
     2401 tctgggttgg aaccggcgag tcgaacgttc gcaatagtgt ttcctttgga gatggcgtct
     2461 acaaatcgac cgacggcggt aagagctgga agaatacagg tctcaaagag actgaacata
     2521 tttctgctat cgccatcaat ccacagaatc cggatatggt tta
//
LOCUS       DASSFP010000414         7718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2211896, whole genome shotgun sequence.
ACCESSION   DASSFP010000414 DASSFP010000000
VERSION     DASSFP010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211896"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..984)
                     /locus_tag="VFH91_09310"
     CDS             complement(58..984)
                     /locus_tag="VFH91_09310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6863236.1"
                     /translation="MIEVFARSSAMRGVVDLAERVSATDSNLLITGESGAGKDLIASF
                     IHSKSRRLSGPFVKIDCATLPGDLVEAELFGYERGAFTGASESKPGRFEAANGGTIVL
                     DEVAHLTNESQAKLLRVIESREFERLGGRATLKLDARLIALTNVELEQAVKRGAFRED
                     LFYRLNVIHIHIPPLRDRREDLKKLAGHFQSQYSRKHGNQVKGISAAALELLLAYDFP
                     GNARELANLVERAVIVAQGKWIEVSDWPKTVIESVQARKRAEVPLSLAEVEAEHIAKV
                     LVASKGNKTECARILGISRKNLYEKIAKYKLG"
     gene            complement(981..1439)
                     /gene="rsfS"
                     /locus_tag="VFH91_09315"
     CDS             complement(981..1439)
                     /gene="rsfS"
                     /locus_tag="VFH91_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET6863237.1"
                     /translation="MKPNSTVKEQSLNPHSAGRTEPETSSPVELDTRILAALHASSEK
                     KALDPVILDLQEIASFTDYFLIVSGTNERQVQAIADEVYESLKKTGSAAARVEGYKTA
                     EWILLDYGDFVVHVFEQKARRFYDLERLWRESKRVEVPAEFTQSSLRTES"
     gene            complement(1417..2100)
                     /gene="nadD"
                     /locus_tag="VFH91_09320"
     CDS             complement(1417..2100)
                     /gene="nadD"
                     /locus_tag="VFH91_09320"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET6863238.1"
                     /translation="MCARRRIGLYGGTFDPVHQGHLDVARRVGKLFEIDEILFVPAQL
                     APHKLVRPVTPAIHRYAMLVLATKDDPQLRISTFELDAPDRRYTVDTLTHFQLTLVED
                     ELFFIMGADSWADIETWRNWEQLLTMTNHIVVTRPGHSVDIEHSPFINRIIDMRGRTQ
                     ISELKDARTPQIFITDVVMDDIAATDIRRFCREGRFDELRGSVPDAVIEYLQKYQIYR
                     NSNEAKLNS"
     gene            complement(2104..3144)
                     /gene="obgE"
                     /locus_tag="VFH91_09325"
     CDS             complement(2104..3144)
                     /gene="obgE"
                     /locus_tag="VFH91_09325"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET6863239.1"
                     /translation="MFIDRAKIHVQGGNGGNGVTAFRREKFVPRGGPSGGDGGRGGDV
                     VLEADQALNTLLHLRYNPRHIAERGLHGEGSNRSGRNGSDLTVHVPVGTQIFDALTGE
                     LLHDLAADKERYLVARGGRGGFGNAHFTTSTNRAPRYHQSGSAGEERDLQLELKLLAD
                     VGLVGFPNAGKSTFISTVSAAHPKIADYPFTTLEPHLGVVDLGDFRTFVIADIPGLIE
                     GAHTGAGLGDRFLRHVERTKLLLHLVDVSSLSGRETVADYETVNRELASYKPELAERP
                     QFVVATKIDALDEIERLTSLKERALRDGRPFYAISSATREGVRDLLQAVATKLKDIKS
                     DERINDAVELAV"
     gene            complement(3217..3486)
                     /gene="rpmA"
                     /locus_tag="VFH91_09330"
     CDS             complement(3217..3486)
                     /gene="rpmA"
                     /locus_tag="VFH91_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET6863240.1"
                     /translation="MAHKKGVGSSRNGRDSHSQRLGLKKFGGEAVLGGNIIARQRGTK
                     WKPGTNVGRGKDDTLFALIDGVVKFEDKGRAGKFVSVYPLEQTAA"
     gene            complement(3499..3774)
                     /gene="rplU"
                     /locus_tag="VFH91_09335"
     CDS             complement(3499..3774)
                     /gene="rplU"
                     /locus_tag="VFH91_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET6863241.1"
                     /translation="MSHAIIKAGGKQFLVEEGQTVRVPSLTADAGASIELEALLTTGD
                     DGKISATVIEHGRQQKIVVFKKKRRKQYKRTRGHRQGYTTLKIDSIS"
     gene            complement(3857..5263)
                     /gene="accC"
                     /locus_tag="VFH91_09340"
     CDS             complement(3857..5263)
                     /gene="accC"
                     /locus_tag="VFH91_09340"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6863242.1"
                     /translation="MSLSARKFKKILIANRGEIACRIIWTCKEMGIKTVAVHSDVDKD
                     SLHVRFADEAACIGPAPSAQSYLNIPAIISTAEIFNVDAIHPGYGFLAESATFAEICE
                     ACNIKFIGPRASVIRLMGDKIEARRAMAAAGLPVLPGSEGAIESEEEGRKLVKEIGFP
                     VIVKASAGGGGRGMRIVRTEAELGHALETASTEAAAAFKDGSVYIERYVEQPRHIEIQ
                     ILADEHGQCIHLGERECSIQRRHQKLMEEAPSTILTPEMRKTMGEAAVEACKKLGYSS
                     AGTVEFLLDPNGKFYFMEMNTRIQVEHPVTEMVTLADIVRNQIRIAEGEDLGYAQDDL
                     LIVGHAIECRINAEDPETFAPSPGTITAFNLPGGPGVRVDTFVYSGYRVSPFYDSMIA
                     KVIVHARTRELAIARMKRALEAMVIEGIKTTIPLHLKIMDDPKFRAGDISTSFMEYFL
                     TRGARPNGLESTVGARTA"
     gene            complement(5349..5918)
                     /gene="accB"
                     /locus_tag="VFH91_09345"
     CDS             complement(5349..5918)
                     /gene="accB"
                     /locus_tag="VFH91_09345"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET6863243.1"
                     /translation="MSAPREEGDNRSARKERTRNAVAKPDASVNMDELRELITLLREN
                     NLAELELERSDFRVRLRREDTVVMAAPGGEPAPAIVRTASAATQAAASPVPVHPGQQA
                     ETQASEDQDLHIIPSPIVGTFYRSPSPTAEPFVKIGSAVKAESVVCIIEAMKLMNEIQ
                     AEASGEVVKIYVENGQPVEYGQPLFGIKQ"
     gene            complement(6188..6649)
                     /gene="rplQ"
                     /locus_tag="VFH91_09350"
                     /pseudo
     CDS             complement(6188..6649)
                     /gene="rplQ"
                     /locus_tag="VFH91_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010285110.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(6659..>7718)
                     /locus_tag="VFH91_09355"
     CDS             complement(6659..>7718)
                     /locus_tag="VFH91_09355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6863244.1"
                     /translation="AMAMDQNNLWTGFQMPRRLAADTETLTERYGRFSAQPFERGFGT
                     TVGNSLRRALLSSIEGAAITAVKIEGVEHEFSSIKGVVEDATDVILNLKQVPFKLHSS
                     EPKTLRISKEEAGEVTAADIETDADVEILDPTVHIATVSEGGSLKIEMRLKRGRGYVS
                     ADRNFDEDLSLGYIPIDSVHTPVKKVNYNVEAARLGQNTEFDKLTIEVWTDGSVKPED
                     SIGLAAKLIKDHMSIFINFEEDTDDFAYAKIERPPLPRNDQLDRSVDELELSVRSYNC
                     LKNADIRTIRDLVQRTEREMLATKNFGKKSLNEIKEILNGMGLDFGMEFDEQGNPIPG
                     TGGRDGADLSDFDNEEEQ"
BASE COUNT         1800 a         2130 c         1851 g         1937 t
ORIGIN      
        1 cgtgttttgc caggccaaat aacccgaaaa cgcatcaaaa agatcgccta accgaatcta
       61 accgagtttg tacttcgcaa ttttctcgta gagatttttg cgactaatgc cgagaattcg
      121 agcgcactca gtcttgttac ctttgcttgc aaccaacact ttcgcaatgt gctcagcctc
      181 gacttccgcc agactcaagg gtacctcagc ccgttttcgt gcctggacag attcaataac
      241 tgtttttggc caatcgctga cttcaatcca tttgccctgc gcgacgatta cggctcgctc
      301 aacgagattt gcaagctcgc gagcgttccc cggaaaatca taagcaagca gcaactcaag
      361 tgcggctgcc gatattcctt tgacctggtt gccatgttta cgcgaatact ggctttgaaa
      421 atggcctgca agcttcttca aatcctcgcg acgatctcgc aagggcggaa tgtgaatatg
      481 gatcacattt agccggtaga agagatcctc acgaaacgct cctcgtttca ccgcctgttc
      541 gagctcgacg tttgtcagcg caatcagacg tgcgtccagt ttcaacgtgg cgcggccgcc
      601 taatctttca aactcgcgcg attcaatgac gcgcagtagc ttggcctgac tctcgttcgt
      661 aagatgagcg acctcgtcga gaacaatggt accgccgtta gcggcttcaa accgtccggg
      721 cttagattca gaagcaccag taaatgcgcc gcgctcgtaa ccaaacaact ctgcttcgac
      781 caaatcacct ggcagagttg cacagtcgat ttttacaaag ggcccactca acctgcgcga
      841 cttcgaatgt ataaaagatg caatcaggtc tttacctgct cctgactcac cagttatcag
      901 tagattcgaa tcggtcgcgc ttacgcgctc agcgagatcc acgaccccgc gcattgcact
      961 tgagcgcgca aatacctcga tcacgattca gttctcaaag agctttgggt gaactcagcc
     1021 ggcacctcaa cccgtttcga ctctcgccat aaacgctcga ggtcgtagaa ccgtcgcgcc
     1081 ttctgctcga agacatgaac aacgaaatcg ccgtaatcca gcagaatcca ctctgccgtt
     1141 ttatatcctt ctacacgagc agctgccgac ccggtcttct taagcgactc ataaacttcg
     1201 tcggcaatcg cctggacctg ccgttcattt gtcccgctga caattaagaa atagtcagtg
     1261 aaactagcga tctcctggag gtccaaaatc accggatcca gagccttctt ctcgctactc
     1321 gcatgcaatg cggccagaat acgagtgtcc agttcgactg gactcgaagt ttccggctca
     1381 gtgcgaccag cagaatgcgg gttcaaagat tgttctttaa ctgttgagtt tggcttcatt
     1441 cgaatttcta taaatctggt atttctgcaa atattcgatc accgcatcag gtaccgaccc
     1501 gcgaagttca tcgaatcttc cttcacggca gaaacgtcta atgtccgtgg cagcaatgtc
     1561 gtccatcacc acgtccgtga taaatatctg cggcgtgcgt gcatccttca attcagaaat
     1621 ttgagttctt cctctcatat cgataatgcg attgatgaaa ggagagtgct caatgtctac
     1681 agagtgacct ggccgcgtta cgacgatgtg attcgtcatt gtcaaaagct gttcccagtt
     1741 tcgccacgtt tcaatgtctg cccaggaatc agcacccatt atgaagaaaa gctcatcctc
     1801 gacgagtgtc agctgaaaat gcgtaagcgt atccactgta tatcgacgat ccggtgcgtc
     1861 cagttcaaat gtagagatgc ggagctgcgg atcatccttt gtggctaaca ccaacatggc
     1921 gtagcgatga atcgccggcg taaccggacg tacaagttta tgtggcgcaa gctgagctgg
     1981 gacgaaaagt atctcgtcaa tttcaaataa ctttccgact cgtcgagcaa cgtcgagatg
     2041 cccttgatga accgggtcaa acgtcccgcc gtacaaacct attcggcgtc tggcgcacat
     2101 tttttagaca gccaattcca ccgcatcgtt aattctttcg tccgacttaa tgtccttcag
     2161 cttcgttgcg acagcctgga gaagatctct tactccttca cgggtggccg acgaaatggc
     2221 gtagaacgga cggccgtcgc gcaacgcgcg ctctttcagt gaagttaggc gctcaatttc
     2281 gtcgagagcg tcaatcttag tagcgacgac aaattggggt ctctctgcca gctccggctt
     2341 gtatgaagca agttcgcgat tcaccgtttc atagtctgca acggtttcgc ggcctgataa
     2401 cgacgacaca tcgaccaggt gtagcaacag ctttgtgcgc tcgacgtgac gaaggaagcg
     2461 atcacctaat ccagccccgg tgtgtgcgcc ctcgatgaga ccgggaatgt cagcaattac
     2521 aaaagtccga aagtctccca gatccactac tccaagatgt ggttcaagtg tggtgaatgg
     2581 ataatctgca atttttgggt gcgctgcaga aacggtagaa atgaatgttg acttgccggc
     2641 atttggaaaa ccgaccagtc caacatcagc caacaacttc aactccagct gcaggtcgcg
     2701 ctcttcaccc gcgcttccgg attggtgata ccgaggcgca cgattcgtag aggtagtgaa
     2761 gtgcgcgtta ccaaatccac ctctgccgcc gcgcgcaacc aggtaacgtt ctttgtcggc
     2821 cgccaaatca tgaagcagtt cgcccgttag cgcgtcaaag atctgagttc ctactggaac
     2881 gtggaccgtc agatccgagc cattgcgtcc ggagcggttg gagccttcac cgtgcaatcc
     2941 gcgttcagca atatgacgcg ggttgtagcg caaatggagt aaggtattca gagcttgatc
     3001 ggcttcgagg accacgtctc cgccacgacc gccatcacct cccgatggac caccacgcgg
     3061 gacaaacttc tcccttcgga aagcagtaac cccgtttccg ccatttccac cctgaacatg
     3121 aattttggcc cgatcgataa acatttaaat caaaagcgac gcccttttgg cagtttgtgc
     3181 tgcctcgagc gtcgcggaac tgcaagtgcg cggtttttaa gcagcagttt gctccaatgg
     3241 atatacgcta acaaactttc cagcacgccc cttgtcttca aatttaacga caccgtcgat
     3301 caaagcaaac aacgtatcat ccttacctcg accgacgttc gtgccgggtt tccatttggt
     3361 acctcgctgc cgagcgataa tgttgccacc caacaccgcc tcaccgccaa acttcttgag
     3421 gccgagtcgt tgcgaatgcg agtcgcgtcc gtttcttgag gaaccaacac cttttttatg
     3481 cgccataact agctccaact aactaattga atcaatttta agagtcgtgt aaccctgacg
     3541 gtggccgcga gtacgtttgt actgcttcct gcgcttcttc ttaaaaacga ctatcttctg
     3601 ttgccgacca tgttcgataa cggttgccga gattttgccg tcatccccag tggtaagtaa
     3661 ggcctcaagt tcgatggaag ctcccgcatc ggcagttaac gacggtactc tgacggtttg
     3721 gccttcttcg acaaggaact gtttaccgcc agcttttatg attgcgtgtg acattgtttc
     3781 tctacctctc agacgcgcca aatgctcgta gaaaagtcgg tcattataag ggaatcgccc
     3841 tccaaacggc aacttctcag gctgtcctcg cgcctacggt tgattccagg ccgttaggac
     3901 gagcaccgcg ggtcaggaaa tattccataa aactggtcga aatgtccccg gcgcggaact
     3961 tggggtcgtc cattatcttc aaatgcaagg gtattgtggt cttgataccc tcaattacca
     4021 tcgcctctaa ggctcgcttc atgcgtgcga tcgcaagctc cctcgtcctt gcgtgaacaa
     4081 tgaccttcgc aatcatcgag tcgtaaaacg gcgaaactcg atagccggag tagacaaaag
     4141 tgtcgactct cactccgggc ccaccgggga gattaaaagc cgtgattgtc ccgggcgaag
     4201 gggcaaaagt ctcagggtct tcagcgttta ttcgacattc gatcgcatgg cccacgatta
     4261 gcagatcgtc ctgcgcatac cctagatctt cgccctcggc tatccggatc tgatttcgaa
     4321 caatatccgc cagcgttacc atttcggtaa ctggatgctc gacctggatg cgggtgttca
     4381 tctccatgaa gtagaacttg ccgttcgggt cgagcaggaa ctcgactgta ccggcgctcg
     4441 agtatccgag cttcttacac gcttctacgg ccgcctcgcc catggtcttt cgcatttcgg
     4501 gagtcaagat tgttgacgga gcctcttcca tcaacttctg atggcgtcgc tggattgagc
     4561 actcacgttc accgagatgt atgcattgtc catgttcatc agcgagaatc tgaatctcga
     4621 tatgacgagg ctgttctaca tatcgctcga tataaacgct accgtcttta aaagcagctg
     4681 ccgcctcagt cgaagctgtt tcgagagcat ggccaagttc agcttcagtg cgcacgattc
     4741 tcatgccgcg tccgccgcct cctgccgagg ctttgacaat gaccggaaag ccgatctctt
     4801 ttacaagctt tcgaccctct tcttccgatt caattgcgcc ctcactaccc ggtaagactg
     4861 gtagaccagc agccgccatg gcccgtcgcg cttcaatctt gtctcccatc aggcgtatga
     4921 cgctggcacg aggtccgatg aacttgatgt tgcatgcttc gcaaatttcg gcaaaggttg
     4981 ccgactctgc gagaaatcca tagccgggat gaatcgcatc tacattgaat atttccgccg
     5041 tactgatgat cgcaggaata tttaaatagc tctgagctga aggcgctggt ccaatgcaag
     5101 cagcttcatc ggcaaaacga acatgcagcg aatctttatc gacatcgcta tgaacggcca
     5161 cagttttaat gcccatctct ttgcaggtcc aaataatgcg acaggcaatc tcgccccgat
     5221 tcgcaattaa gattttcttg aactttcgtg cgctgagtga cattggcttg aatgtaaaag
     5281 gtaacgtcca acttcttaag tcgtggggct aacccatcgg tgacccggaa gttggacgtg
     5341 aagccctgct actgtttaat accaaagagt ggctgcccgt attccacagg ctgaccattc
     5401 tctacgtaaa tcttcacaac ctcacccgag gcctctgcct ggatctcatt catcagtttc
     5461 atcgcctcga taatgcagac caccgattcg gccttaaccg ctgaaccaat ctttacaaat
     5521 ggctccgccg tgggtgatgg agagcgataa aacgttccga caattggcga aggaatgata
     5581 tgcaggtcct gatcttcgga ggcctgggtt tctgcctgtt ggcccggatg taccggaacg
     5641 ggcgaagcag cagcctgggt cgcagcagac gctgtccgga cgatagcggg cgccggctcg
     5701 ccgccgggcg cggccatcac aacggtatct tcccgcctca accgcacgcg aaagtcagag
     5761 cgctccagtt caagttcagc aagattattc tcgcgaagaa gagtaatcag ttcccgcaat
     5821 tcgtccatgt tgacggacgc atcaggttta gcgaccgcat tcctggtgcg ttccttgcgc
     5881 gcgcttcgat tgtcgccttc ttctctgggt gctgacatga attgttcgcc gtgaataaac
     5941 ctttatagct gtggaatagc agtaaaaggt tgggccccaa aagactcgtg cgtgactgcc
     6001 gaagatcagt tggggctcaa aatcaaaccg gcgtggccgg gttactttac tactcctcag
     6061 ttgaatcggc tgtttcggag gagtcttctt ttttggctcc tgccttcttt gaatcagacg
     6121 atttcttctt cttcgaagct gcgctcttgg cgcgtttctt ttctgactca atcgcctcga
     6181 tttcacgagg gttatccact aattcaataa tagctaactc cgctcgatca ccttcgcgat
     6241 gacccaactt gaggatgcgc gtataaccgc ccggccgatc tttgtagcgc tcacccagtt
     6301 cgttgaaaag tcgacgaagg gcggcaactc ctgctgttct ttcaggccga agctgtcccc
     6361 gttttccagt gatcgccgac attgctgtat ttccggcatg aaagaaaaca gcagcctgac
     6421 ggcgaagatg cagagcacgc gcgtcggaac cgtcgcctga caaactctgt gcgcgtctgg
     6481 aaagagtaat agcgcgctcg ataaacggcc ttaactcttt cgccttgggc aaagttgtaa
     6541 cgatgcgctc ttctcgcgag ttgattaaag aagtcgccag attacggagc atggaatcgc
     6601 gatgctcacc ggtccgaccg agtttgcgat gtgcctttag atgtcgcatg gcgcttgact
     6661 actgttcctc ttcattatcg aaatctgaca ggtccgctcc atcgcgaccg ccggttcccg
     6721 gaatgggatt accctgctcg tcgaactcca taccgaagtc gagacccatg ccattgagaa
     6781 tttccttgat ctcgttgagc gacttcttgc caaagttctt ggtggctagc atttcgcgct
     6841 cagtcctttg gaccaggtct ctaattgttc gaatatctgc attctttaag cagttatagg
     6901 aacgaaccga aagctcaagc tcatctactg agcggtccaa ctgatcattt cgcggaagcg
     6961 gaggacgttc aatcttggcg taagcaaaat cgtcggtgtc ttcttcgaaa ttgatgaaga
     7021 tggacatgtg gtccttgatc aattttgcag ccagaccgat tgagtcttcg ggtttaaccg
     7081 aaccatcagt ccacacttcg atagtaagct tgtcaaattc cgtgttttga ccgagacgag
     7141 ctgcctcgac gttgtaattg accttcttga caggagtgtg aaccgagtcg atgggaatat
     7201 aaccaagcga caggtcctca tcgaaattac ggtctgcaga aacgtatccg cgaccgcgct
     7261 tgagacgcat ctcgatcttg agagagccac cttcactaac ggtcgcgatg tgcacggtcg
     7321 gatccaggat ctcgacatcc gcatcagtct cgatatcggc agcagtaact tcgcctgctt
     7381 cctctttcga gattctcaaa gtctttggtt cgctgctgtg cagtttgaag ggcacttgct
     7441 tcaaattcag aatgacgtcg gttgcgtctt caacgacacc cttaatcgaa gagaactcat
     7501 gttcaactcc ctcgattttg acagcggtaa tcgcggcgcc ttcaatagat gaaagtaacg
     7561 cacgtcgtag cgagttacca acagttgttc cgaatcctct ttcaaacggt tgggccgaga
     7621 atctgccgta gcgttcagtc aacgtttcgg tatccgccgc caaacgacgc ggcatttgaa
     7681 atccggtcca gagattattt tggtccatag ccatagcg
//
LOCUS       DASSFP010000415         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2067537, whole genome shotgun sequence.
ACCESSION   DASSFP010000415 DASSFP010000000
VERSION     DASSFP010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067537"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFH91_09360"
     CDS             <1..223
                     /locus_tag="VFH91_09360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6863245.1"
                     /translation="DKLVITISSRGIVPTNVGTNGAITTKPVPDEGAEERRGWGLRLI
                     RTLMDEVEFERVDEGTSLRMTKYLRSRSS"
     gene            325..702
                     /locus_tag="VFH91_09365"
     CDS             325..702
                     /locus_tag="VFH91_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6863246.1"
                     /translation="MEIISGAVRSHCVGTTAVIYASDYLNKLTGEKIERECRRQLDSG
                     CRALVIDFSDTKLVNSIGISILLGVINVAEKSGARIVFSDVNIQTIQLFEMLGLTRHV
                     VLAKNEQEALSNLSGFDAKTVAH"
     gene            707..1966
                     /locus_tag="VFH91_09370"
     CDS             707..1966
                     /locus_tag="VFH91_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6863247.1"
                     /translation="MEPSTQKLIHTFGALADLGQEVANTGDFAAMVRTSMHLLLGTLA
                     LRRGAVVECSPGDSLQCVAIWGLDDAVAKELSIDETERAAFLIEDDCALSVSDSGQSN
                     SFITRHRERLTQEGIELVIPMIVRNETTGFVLLGGKASGEEFSPEDFETIRAMVRHIG
                     VGIHTHRLLEQLAQRAEENLRLYKDLRAIYRDTVKAFAAAIDIKDKYTQGHSERVGRY
                     SEIIATELGWSEDEVEGIQIAGYLHDVGKLIVERDIINAPYQIDAKQSTELNRHPAAG
                     YEILRPINHPYADIPLMAKYHHERLDGRGYPDGLTDEQIPLGAKIVSLADSFDAMTTD
                     RPYRRRRSFPDVVRDLRQNSGKQFDGKVVAAFARAMLKEVNGDAKDRKIMKMLGKGYI
                     NPEEAALLLRDLIDELDSEAKVKAKSL"
BASE COUNT          550 a          485 c          550 g          482 t
ORIGIN      
        1 tgataaatta gtcatcacta tttcgagccg cggcatcgtg ccaaccaatg tcggcacaaa
       61 cggcgcgatt acaactaagc cagtacccga cgagggagca gaagaaaggc gcggttgggg
      121 actcagactg atccggacac tcatggatga agtcgagttc gaacgggttg atgaaggtac
      181 aagcctccgt atgacaaagt acctccgtag ccgctcttca taacgggatt cgatgtgata
      241 taggtctact ggaagatcac atccattaag ttacaaagtc ttacaacgat tatttttatt
      301 ttcaaggcag aagggacctg tctagtggag ataataagtg gcgcagtacg ttcgcactgt
      361 gttggcacta cggctgtaat ctatgccagt gactacctca acaaattgac aggcgaaaag
      421 atcgagcgcg aatgccggcg acaactggat tcaggttgtc gtgcgctggt tatcgacttc
      481 agcgacacta aactcgtcaa tagtattggt atctctatcc ttctcggtgt tatcaacgtc
      541 gccgaaaaaa gcggcgcccg catagttttt tctgacgtaa acattcaaac tattcaactc
      601 tttgaaatgc ttgggttgac ccgacacgta gtgctcgcaa agaacgagca agaagccctc
      661 tcgaatctct caggttttga cgcaaaaaca gtagcccatt gattagatgg agccatcgac
      721 ccaaaaactg atccatacgt tcggtgcgct agcggatctg ggccaggaag ttgccaacac
      781 cggcgacttt gcggcaatgg tacgcacctc gatgcacctc ttattgggaa ccctcgccct
      841 gcggcgtggg gcagtggttg aatgtagccc cggcgattcc cttcaatgtg tcgcgatttg
      901 gggcctcgac gatgccgtcg ccaaagagtt gtcgattgat gagaccgaac gagcggcgtt
      961 cctaattgaa gatgactgtg ccttgagcgt cagcgatagt ggacagagta atagcttcat
     1021 aacgcgacac cgtgagcgtc tcacccagga gggaatcgag ctggtgattc ccatgattgt
     1081 tcggaacgaa acaaccggct ttgtcttact tggtggtaag gcgagtggcg aagaattctc
     1141 tcccgaagat tttgagacga ttcgtgcaat ggtccggcac attggtgttg gaattcatac
     1201 tcaccgccta ctcgaacagt tggcccagcg cgcagaagag aacttacgtc tgtacaaaga
     1261 cttgagagct atctatcggg acactgtaaa agctttcgcc gccgcgatag acatcaaaga
     1321 taagtacact caaggacatt cggaacgagt cggtcgctac agcgagatta ttgcgacaga
     1381 attgggttgg agtgaagatg aggtcgaagg gattcagata gcagggtatc ttcacgacgt
     1441 tggcaaactg atcgttgaac gcgacataat caacgctcca tatcagatcg acgcaaaaca
     1501 atcgaccgag ctgaaccgcc atcccgccgc tggatacgaa attctcagac cgatcaatca
     1561 cccttatgcc gacattccat tgatggcgaa gtatcaccat gaacgactgg atggccgagg
     1621 atatcccgat ggtctaacag acgaacagat tccgttgggt gcaaaaatcg tctcgctcgc
     1681 tgactcattt gatgccatga cgacggatcg tccttaccgg cgtcgacgct cgtttcctga
     1741 tgtagtgcga gacttgaggc aaaacagcgg aaagcaattt gacggcaagg tcgtagctgc
     1801 gtttgccaga gcgatgctga aggaagtgaa tggcgatgcg aaggaccgga agatcatgaa
     1861 gatgctcggt aagggttata tcaatcctga agaggctgcg ttgcttctga gagatctgat
     1921 tgacgaactc gactccgaag caaaggtcaa agcaaagagt ttgtagcaaa ggtcagtacc
     1981 gaatcgcggt agcgtcgggt gactctccgg tacgtcaagt tgtgatattt ggagtagtgt
     2041 cctaatctaa gttttctctg tgctatt
//
LOCUS       DASSFP010000416         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1014057, whole genome shotgun sequence.
ACCESSION   DASSFP010000416 DASSFP010000000
VERSION     DASSFP010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014057"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFH91_09375"
     CDS             <1..325
                     /locus_tag="VFH91_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863248.1"
                     /translation="GTYMVAVLAREHGIPFYVAAPLTTLDMTLKSGAEIPIEEREARE
                     ITHIKDHQLAPDGISVHNPAFDVTPNKLIAAIITDKGVARAPYEESLKKLFQPEATRT
                     SAQHK"
     gene            complement(490..1449)
                     /locus_tag="VFH91_09380"
     CDS             complement(490..1449)
                     /locus_tag="VFH91_09380"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6863249.1"
                     /translation="MGLTGIALVTGGAGFIGSHIASALLASGARVRVLDDLSTGHREN
                     IDEIGSRVDFIEGSVADPQALDRALDGVELVFHEAAIPSVPRSVENPLQTHVASIDGT
                     FSLLLAARDKKVRRLIYAASSSAYGDQPTLPKVEDMSPDPLSPYAVAKLVGEYYCKVF
                     SRVYDMETISLRYFNVFGPRQDPGSQYSGVVSRFISCLFNKERPVIYGDGEQSRDFTY
                     IDNVVSANLKAAEAKEGIGHVFNIANGERVTLNQLLAELKDLTGNPQVTAEHKEPRVG
                     DVRHSLADVTAAKQLLGYESKVGLRDGLQRTIDWWKTSRFAKA"
     gene            complement(1492..2790)
                     /locus_tag="VFH91_09385"
     CDS             complement(1492..2790)
                     /locus_tag="VFH91_09385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET6863250.1"
                     /translation="MQIAVIGSGYVGLVTGACFAEFGVDVACVDVDEQKIERLLNGVM
                     PIYEPGLEHLVAKNSQAGRLRFTTDVRQAVEQALVIFLAVGTPPLPDGSPDLSHVESA
                     AAAIADYMNGYKVVVTKSTVPIGTGEHIRTLINERKKTRANFGVVSNPEFLREGAAIN
                     DFMRPDRVVIGSRDEEAIAIMKDLYRPLYLIEAPFVITSLEAAELTKYAANAFLATKV
                     SFINEIANLCDKIGCDVHDVARGIGMDKRIGSKFLHPGPGFGGSCFPKDTRALSTVAR
                     KFDSESLIVDAVIEVNRRQAKAMLSKIRNLVGNFESKNIAVLGLAFKPETDDMRDAPS
                     IGIIRHLIESGAKVRAYDPVAKDEAIKILPEVSYADDEYTAVEGADALVVITEWNQFR
                     ALDMTRVRDLMKTPKVADLRNIYEPEDMREMGFEYVGVGR"
     gene            complement(2946..>3606)
                     /locus_tag="VFH91_09390"
     CDS             complement(2946..>3606)
                     /locus_tag="VFH91_09390"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET6863251.1"
                     /translation="SIAAFVAVAVLSVYSMVVLAAPGTKASGDLSVTGQVTVNGQKVV
                     SGGTLFSDSTISTSDQSSATVSISKLGRVELAPNSNLRLSFTDNTVTAMLETGSAHVS
                     TLKGINVNLTTNDGAVLVDGSQATSFTASASRGRTSVATQAGVAQLRAGGSVKQIAAG
                     ESATAGSPNPQDKDEGLSGGALAVLLLAVGGAVAGVLYAALHNNDLNFGGSVTVVSPT
                     K"
BASE COUNT          905 a          979 c          869 g          853 t
ORIGIN      
        1 tggcacatac atggtggcag tcttagcgcg agagcacgga ataccgttct acgttgcggc
       61 cccactcacg acacttgata tgacgctgaa gagtggagca gagattccga ttgaagagcg
      121 tgaggcaaga gagataaccc acatcaagga ccatcagctc gcacctgacg gaatctcagt
      181 tcacaatccc gccttcgatg tcacacctaa taagctgatt gcggcgatta tcactgacaa
      241 aggggtggct cgagcgccgt acgaagagag tttgaagaag ttgtttcagc ctgaagcaac
      301 aagaacttca gcacagcaca aataatccct gacaccaccg acttcaaaga ccgtgtgaag
      361 actcaagaat acaatccaca gattacgccg attacacaga ttctttacca gaacgccttt
      421 gcgccgtgcc tttctctgaa gaaatctgtg taatcggcgt aatctgtgga tagtattctt
      481 gagccgcagc taggccttcg cgaagcgact ggttttccac cagtcaatcg tgcgctgcag
      541 tccatctcgt agaccaactt tcgactcata accaagaagt tgtttcgctg ccgtcacatc
      601 agcaagactg tgacgcacat cacccacacg cggttcttta tgttcggctg ttacctgcgg
      661 attccccgtc agatccttca attccgccag cagctgattc aaagtcacac gctcgccgtt
      721 tgcgatattg aagacgtgtc caattccctc tttcgcttca gcggctttca agttcgccga
      781 gacgacgttg tcaatatagg tgaagtctct cgactgttct ccatcaccat aaataactgg
      841 acgttctttg ttgaacagac aactgataaa ccgagaaaca actcctgagt attgcgaacc
      901 aggatcctga cgtggtccaa atacattgaa atatctaagt gagatcgttt ccatgtcgta
      961 gactcgcgaa aagaccttgc aatagtactc ccctaccagt ttggcaacag cgtaaggtga
     1021 caagggatct ggagacatgt cctcgacttt tggcagcgtc ggctggtctc catacgctga
     1081 tgatgacgcg gcgtaaatca gccttcgaac cttcttgtcg cgggcggcca gcaacaacga
     1141 aaaagttcca tcaatggacg ctacgtgagt ttgcaacgga ttctcgacgg agcgcggaac
     1201 tgaagggata gcggcctcgt gaaaaaccag ttcgactcca tctaatgccc gatccaacgc
     1261 ctgcgggtca gccacgctgc cctcaatgaa gtcgactcgt gaaccaatct catcgatgtt
     1321 ttcgcgatgg cctgttgaga gatcgtcaag cactctgacg cgcgcaccac tggcgagaag
     1381 agctgatgcg atgtgagaac ctatgaaacc tgcgccgcct gttactagtg caattcctgt
     1441 caatcccatt cgtatctaat atccttgtct gagtactctt cagagtttca actatcgtcc
     1501 cacaccgaca tattcaaagc ccatttcgcg catatcctcg ggctcgtaaa tattacgtag
     1561 atcggctacc ttcggcgtct tcatcaagtc gcgcacgcgc gtcatgtcaa gcgcgcgaaa
     1621 ttgattccat tcagttatga caaccagagc gtccgcgcct tcgactgccg tatattcgtc
     1681 gtcggcatac gagacttccg gcagaatttt tattgcctcg tcttttgcta ccggatcgta
     1741 ggcgcgcacc ttagcgccgc tttcaatcaa gtgacggatg atgccgatgg aaggggcgtc
     1801 gcgcatatca tccgtttcag gtttgaaagc aagaccaagc accgcaatgt ttttgctttc
     1861 gaaattcccg acaagatttc gaatcttcga gagcatggct tttgcctgac gacggttgac
     1921 ttcaataact gcgtcgacaa tcagcgactc gctatcgaac ttcctcgcta cagtcgacaa
     1981 agccctcgtg tctttgggaa aacacgagcc accgaagcct ggaccaggat gcagaaactt
     2041 actgccgatt cttttgtcca taccgatgcc gcgcgcgacg tcatggacat cgcagccaat
     2101 cttgtcacag agatttgcaa tctcattgat gaacgacact tttgtcgcca gaaaagcgtt
     2161 tgctgcatat ttggtcaact cagctgcttc aagcgaagtg atgacaaacg gagcttctat
     2221 taagtaaagc ggtcgataaa gatctttcat tatcgcgatc gcttcttcgt cccgactgcc
     2281 aataacaact cgatctggcc gcatgaagtc gtttatcgcg gcgccctcac ggagaaactc
     2341 aggattcgaa acgacaccaa aattggcacg agttttcttg cgttcattga taagtgtgcg
     2401 gatgtgttcg ccggtgccaa tgggcactgt cgactttgtt acgacgactt tgtagccgtt
     2461 catatagtcg gcaattgcgg cagcggccga ttcgacatgg cttagatctg gtgagccgtc
     2521 cggcagggga ggagttccga cagcgagaaa gatgaccaga gcctgttcaa cagcctgacg
     2581 gacatcggtg gtgaacctga gtcgtccggc ttgagaattt ttggcaacaa gatgttcaag
     2641 tccgggctcg tagattggca ttacgccatt caagagtcgc tcaatctttt gctcatcgac
     2701 atcaacacac gcgacgtcga cgccaaattc ggcgaagcat gctccggtaa ccaaaccaac
     2761 ataaccacta ccaattactg ctatttgcat ttattgagac tcgcgctttg gtggaaaata
     2821 ttaactgaca atcaaaacct tgcgggtcac gcaccactct tgtcgcgtga cccgcaaggt
     2881 caaatcgatt gactgcagcg gataaatatg ggaagccgca gtattttttt gtgcactcga
     2941 cgaacttact tagtcgggct gaccacagtt accgaaccac cgaagttcag gtcattgttg
     3001 tgaagagcgg catagagaac acccgcaaca gcaccaccaa cggccaggag cagcactgca
     3061 agtgcaccac cgctcaaacc ttcgtccttg tcctgtgggt tcggggatcc ggcagtggca
     3121 ctttcgccag cggcaatctg cttaacgctg ccacccgctc gaagctgcgc gacaccagcc
     3181 tgagtcgcga ctgaagtgcg accacgtgat gcactggcgg tgaacgatgt agcctggctc
     3241 ccatcaacaa gcacggcacc atcattggtc gtcaaattca cgttaatccc ttttaacgtc
     3301 gaaacgtgag cgctacccgt ctcaagcatt gcggtaactg tgttgtccgt gaaactcaaa
     3361 cgcagattgc tattaggggc gagttctaca cgaccgagtt tgctgatgct aactgtcgcg
     3421 ctgctctgat ccgaggtgct gatggtagag tcggaaaaga gggtcccacc ggaaacgacc
     3481 ttttggccgt ttactgtaac ctggccagtg acagacagat cgcctgaggc cttggttccc
     3541 ggcgccgcta atacaaccat tgagtaaaca ctcaaaacgg ctactgccac aaaagccgcg
     3601 atggac
//
LOCUS       DASSFP010000417         6305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1615652, whole genome shotgun sequence.
ACCESSION   DASSFP010000417 DASSFP010000000
VERSION     DASSFP010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615652"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..765
                     /locus_tag="VFH91_09395"
     CDS             34..765
                     /locus_tag="VFH91_09395"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET6863252.1"
                     /translation="MKIYPYCLVSSRVLLPASIPGLDGRPILQQDIGGLTVILSESDA
                     EKVALTRENVLRHDAVVRRVLEHETPLPFRFGHLVTLEQLTSYIESKSESLQARLQQL
                     RGRVEMSVKIIWNRESKLTNSNPESETVPGNTGKGVAFLMSKRAEMRQEEALSLEAEA
                     VAAWLKSEISGFVCEERVVLRPTEKLVLSASFLVDASKLEVYKKRTALIRLGRPELHF
                     LTSGPWPPYTFSNNELEFKTHLGVS"
     gene            765..1130
                     /locus_tag="VFH91_09400"
     CDS             765..1130
                     /locus_tag="VFH91_09400"
                     /inference="COORDINATES: protein motif:HMM:NF016977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HET6863253.1"
                     /translation="MEFSPENPLSVEPEIDTSHAEEFAEQLRHLGDTLPSRINVDPKS
                     VEQGLAKLVLTLIEFIRRLLEKQAVRRMEGGDLSPEQIEELGLALMKLEEKLHELKTQ
                     FGLSEEDLNLDLGPIGRLI"
     gene            1215..1610
                     /locus_tag="VFH91_09405"
     CDS             1215..1610
                     /locus_tag="VFH91_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HET6863254.1"
                     /translation="MSSERSTLITARPPGLVDVLDRILDKGLVIAGDIKVSLANVELL
                     TLQIRLLVCSIDKAEQIGLNWWRYDTNLTTRAERAEAENLELRERLNELEREIRKLGV
                     SGAAVSGPELSPRSARQQAGTRAKQKKRS"
     gene            1717..2001
                     /gene="gvpA"
                     /locus_tag="VFH91_09410"
     CDS             1717..2001
                     /gene="gvpA"
                     /locus_tag="VFH91_09410"
                     /inference="COORDINATES: protein motif:HMM:NF006874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_component: GO:0033172 - gas vesicle shell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle structural protein GvpA"
                     /protein_id="HET6863255.1"
                     /translation="MAVERAPGGTSLIDVLDRVLDKGIVVDAWVRISLVGIDLITVEA
                     RVVVASIDTYLKYAEAVGLTPAVSRPALTPGAEPMEGETRRRVTVEVDKK"
     gene            2053..4218
                     /locus_tag="VFH91_09415"
     CDS             2053..4218
                     /locus_tag="VFH91_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6863256.1"
                     /translation="MPTELSFRATPPIADSEDIGAHLRQYDVFIETTTQMLVTPKLQE
                     RLLLALEAIVNIFGYRQAAIALINERDAVLQIRAAIGFDSDQSATRVEMPIDSGAGCV
                     RVIHDGHYSWISLNQDLASRTLFEKMHWEGDVLALPLFGIPEMSPKGSRTSSSSSRLS
                     NQYWTFEPGLRLGVLYIGAFEANVDPISLSLLKRFADRIGVIASLAIQQERLVTTVTK
                     LQRERQWIESIMKSVADPIVLTDLDNEILLQNRRAEELFSGSANAGEGKRRALKMNDL
                     LFSAYLSSAAVSSSELVQRDITLVDPIEGSDIHFEVISTPAVNSRGEPLGLVSIFRDV
                     TDLREANEELARNFGKLQRAEAESRRERDRLDLIIENVGHPIVVCDASGKFILFNRRA
                     ELLFQENESFPARAASAVRTNAVKFTSFISGLASGFETGRQAEIELIDPELGRTLPME
                     ITSREVLDMTGQVTAVVSVLHDLTEIRELERRRVEQQLFESEKLAAVGRLAASIAHEV
                     NNPLEAIKNALYLMQSGSEGDKNARFLQIARKETERVSHIIRQMLGFARSSGEVDWVD
                     VNQVLEETMVLLEKKLKQSHIDVDVKFDQFLPPIRARADQLRQVFLNLLINAQQAIQG
                     EGRISIATSRYEQALQPSIHIQVSDTGIGIAEDDLTRIFEPFFSTGKKGTGLGLWVTQ
                     DIVRQHGGRIEVNSEPGKGTTFSIILQVDSPILEFQEKK"
     gene            4234..5904
                     /locus_tag="VFH91_09420"
     CDS             4234..5904
                     /locus_tag="VFH91_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6863257.1"
                     /translation="MNQPADRNIPPVITDAALDTFSDQGRLLVVDDEESLRITTAAIL
                     EQDGYIVDTAASGDEAISLLAQTQYDLVLTDLHMEGGDGLSVLALIRQKAPFTISVVL
                     TGFASVESAIAALQEGAYDYLTKPCDIDHMRHTIKRGVEHRRLMLAEQKARADLKQLN
                     RDLEARIEERTAQLTMLNAELAEANRAKDVFLATLSHELRTPLTPVVGWIKLLRGGNL
                     DTSGVVQALDAIERNAWLQSRLIDDLLDTSRIATGKLRCDLLPTDLNVIVKATVDTVK
                     ASASTRNIDLDMELTTSPIIVLGEPVRLQQIAWNLVSNAIKFTESGGRVRVLTSTNGI
                     DATFVVEDSGIGIEPDFLPHVFDRFRQADGSTSRVHGGLGLGLAIAHALAKMHSGQLE
                     AYSEGVGKGARFTLRLKLAIGAKVDPEVTTKQEHSFAGLEVLIVEDSPDTLTLLSTIF
                     SQEGANVAAATSANDALNRILEKRPDVIISDIGMPDTDGYKFLEKVRELPAARHIPAI
                     AVSGYASDEDRKRAVSVGYAALIPKPIDVNELFETINELGIGELSSKE"
BASE COUNT         1568 a         1494 c         1695 g         1548 t
ORIGIN      
        1 gagagtgtcg ctgacaaggt gaagtagttt gacatgaaga tttatcccta ttgccttgtg
       61 agcagtcgcg tacttttgcc agcgtccatt cctggattag atggacgccc catccttcaa
      121 caggacattg gagggttgac cgtaatttta agtgagtccg acgctgagaa agttgcgttg
      181 acgcgcgaga acgtgttacg gcacgacgcg gtagttcgga gggtactcga gcatgaaacg
      241 ccactaccct tcaggtttgg acatctcgtt acgctggaac agctcaccag ttatatcgag
      301 agcaagtcag agtctctgca agcgcgactt cagcaactgc ggggtcgagt agagatgagc
      361 gtcaaaatca tctggaatcg cgaaagtaaa ttgacgaata gtaaccctga gtctgagaca
      421 gttccgggca acactgggaa gggagtcgca tttctaatgt ctaagcgtgc cgaaatgcgg
      481 caggaggaag cgttatcttt ggaggctgaa gccgtagcag catggctaaa atcggagatt
      541 tctgggtttg tttgcgaaga gcgcgtagtt cttcgcccga cggagaaatt ggtcttgtcg
      601 gcgtcgtttt tggttgatgc ctcgaagctt gaggtgtaca aaaagcggac ggccttgatt
      661 aggttgggtc gtccagagtt gcattttctg accagcggac cctggccgcc ttatactttt
      721 tcaaataatg agcttgagtt taaaacacac ttgggagtaa gctgatggag ttttcccccg
      781 aaaaccccct ttcggttgaa cctgagatcg atacttctca cgctgaggag tttgctgaac
      841 agctccgtca ccttggtgac actctgccat cgcggattaa cgtggatcca aaaagcgttg
      901 agcagggtct ggcaaagctg gttttaactt tgattgagtt catccgtcgc ttgttggaaa
      961 agcaagcagt tagacgtatg gaaggcgggg acctctcacc tgaacaaatc gaagaattgg
     1021 gactggcatt gatgaagttg gaagagaaac ttcatgagtt aaaaacccaa tttgggttga
     1081 gcgaggaaga tctcaatctc gatctcggtc ccatcggtcg tttgatttag taatcttaag
     1141 cacgatagac cattacatgg tggcggccca ccagcgctgt ttacaaagcc gcccccttaa
     1201 tgtggagggc gaaattgtcc agcgaacggt ctacccttat aacggctcgt cccccgggcc
     1261 ttgtcgatgt tctcgataga atcctcgaca aagggttagt gattgccggt gacatcaaag
     1321 tgtcgctggc caatgtcgag cttctcactc tgcagattcg attactggtt tgttcgatcg
     1381 ataaagccga acaaatcggc ttgaactggt ggaggtatga cacgaatctg actactcgcg
     1441 cggaacgagc tgaagcagag aatctggagc ttcgagaacg actgaacgag ctcgaacggg
     1501 aaataagaaa actgggggtc tctggcgcag ctgttagtgg acctgagctt tcaccacgat
     1561 cggcgcggca gcaggccgga acaagggcaa aacaaaaaaa acgttcatag aaagcttgga
     1621 gatgtaatcg taaccctgtg gccttaatat ctaaacaagc accgtcgact ctaggccgcg
     1681 tgctgatttt caaacttcta aaaaggaccc aagcaaatgg ctgtcgaacg agcaccaggc
     1741 ggtactagtc taatagatgt tctcgatcgc gtcttagata agggaattgt ggttgacgcg
     1801 tgggtgagaa tatcccttgt cggtatcgac ttgatcacag tagaagcgcg agtcgttgtg
     1861 gcttcgattg atacttatct aaagtatgcc gaggcggtcg gacttactcc cgctgtttct
     1921 cgccccgctt tgacgcccgg tgcggagcca atggagggtg agacgagacg tcgagtcacg
     1981 gtcgaagttg ataaaaagta gcaaccagac agatattagg atactggctg gctcaattcg
     2041 tttatggggg aagtgcccac agaattatcc tttcgagcaa caccgcccat cgcggactct
     2101 gaagacatcg gtgcccatct gcgccagtac gatgttttca tcgagaccac gacgcaaatg
     2161 ctcgtcactc cgaaactcca ggagcgcctc ttactcgccc ttgaagctat cgtcaatata
     2221 ttcggttatc gccaggctgc aatagcactc atcaatgagc gcgatgccgt actacaaatt
     2281 cgagcagcca tcggttttga ttctgatcaa tccgcgactc gagtagaaat gcctatcgat
     2341 tcaggcgctg gttgcgtacg agtaattcat gatggtcatt actcgtggat ttcgctcaat
     2401 caggatctag cctcgcgaac tctcttcgaa aaaatgcatt gggagggaga tgttcttgcg
     2461 ctgccgctgt tcgggattcc cgaaatgtcg ccaaaaggct cacgtacttc ttctagttcc
     2521 tctcggcttt cgaatcaata ctggacgttc gaaccgggtt tgcggctggg ggttctttac
     2581 attggcgcat ttgaagccaa tgtagatccg atctccttga gcttgctgaa gcgctttgcc
     2641 gatcgaattg gagtcatcgc ctctctggca atacagcaag agcgacttgt caccacagtc
     2701 accaagctcc aacgtgaacg gcagtggatc gagtcgatca tgaagtcggt ggcagacccg
     2761 attgtgttga cggatctgga taacgaaatt cttctgcaga accggcgcgc agaagaattg
     2821 tttagcgggt ccgcaaacgc tggcgaagga aaacgccggg cgctgaagat gaatgatctg
     2881 cttttttcag cctatctttc cagtgccgca gtttcgtcct ctgaactggt ccaacgtgac
     2941 atcacgttgg tcgatccgat tgaaggttcc gacattcact tcgaagtgat ctcgacgccc
     3001 gcagtaaact cgagaggtga accgttgggc cttgtttcta tcttccgcga cgttactgac
     3061 ttgcgagaag caaacgaaga gttggcgcgc aactttggca agctacaacg agcagaagct
     3121 gaatctcgcc gcgagcgcga tcgcctcgac ctgattatcg agaacgtggg ccatccaatc
     3181 gtcgtttgtg acgcatcagg caagttcatt ctcttcaatc ggcgagcaga actactcttt
     3241 caggagaatg aatctttccc agcgcgtgcc gcgtcagccg tgcgaaccaa cgcggtcaag
     3301 ttcacctcgt tcatctcggg acttgcttcc ggcttcgaaa caggtcgtca agccgagatc
     3361 gagttgatcg atccggagtt gggccgcact ctgccgatgg agataacgtc tcgtgaagtt
     3421 ctagatatga ccggacaggt aactgcggtg gtttccgtgc tacatgacct tacggagatt
     3481 cgcgaactgg aacgacgtcg agtcgaacag caactgtttg agtcagagaa actagcggcc
     3541 gtgggacgtc tggcagcttc aatcgcccat gaagtcaaca atccactcga agcaattaag
     3601 aacgcactct acctgatgca gtcagggtct gaaggcgaca aaaacgcccg ttttcttcag
     3661 atcgctcgga aagagactga gcgcgtatcc catatcatcc gacagatgtt gggttttgct
     3721 cggagctccg gcgaggttga ttgggttgac gtgaaccagg tgcttgaaga gactatggtg
     3781 ctgctggaaa agaaattgaa acaatcgcac atcgatgtcg acgtgaagtt cgaccagttt
     3841 ttacctccaa taagagcacg cgccgatcag ctgcgccagg tgtttcttaa cttactcatc
     3901 aatgctcaac aggctattca aggcgaaggc cgtatctcaa tcgcgacctc acgttatgaa
     3961 caagcgctac agccatccat tcatattcaa gtcagtgata ctggcattgg aatagccgaa
     4021 gatgatctga cccgaatctt cgaaccgttc ttttccaccg ggaagaaggg taccggtctt
     4081 ggactctggg tgactcaaga cattgtcaga caacacggcg gacgcattga agttaacagt
     4141 gaacccggta agggaacgac cttcagcata attctccagg tggattcccc tatcttagaa
     4201 tttcaggaga agaagtagcg tagtgggacg ccaatgaacc agccagccga tcgtaatatt
     4261 ccgccggtga taactgatgc cgcactcgac acctttagcg atcagggaag gttgttagtt
     4321 gtcgatgacg aagagagttt aagaattacg acagccgcga ttctggaaca agacggctac
     4381 atcgtagata ccgcggcgag tggtgatgaa gcgatctcgt tgctggccca gacgcaatat
     4441 gatctggtcc tgacagacct tcatatggaa ggcggtgatg gactatccgt cctcgctctg
     4501 atccggcaga aagcgccttt tacgatttct gtagtcttga cgggttttgc ctcggtcgaa
     4561 tcagcgattg cggcgttaca ggagggtgcc tacgattacc ttaccaagcc gtgtgatata
     4621 gaccacatgc ggcacacgat caagcgaggt gtagagcatc gacgtttgat gttggcggaa
     4681 caaaaagctc gcgcagacct taaacagctt aatcgtgacc tcgaagcgag aattgaagaa
     4741 cgaacggcgc agttaactat gctcaacgcg gaactggctg aggccaaccg cgccaaagat
     4801 gtgtttcttg ccacgctttc tcacgaattg cgcacacctc tgacgccagt tgttggctgg
     4861 attaagttgc ttaggggtgg caacctcgac actagtggag ttgttcaggc gctggatgcg
     4921 attgagcgaa acgcctggct tcagtcgcgc ctgattgacg atcttcttga tacttccagg
     4981 attgcgactg gtaagctccg gtgtgattta ctgccgacag acttgaacgt gatcgtcaaa
     5041 gctactgtcg acaccgttaa agcatcggca tctacgagaa acattgacct cgatatggag
     5101 cttacgactt cgccgattat cgttttgggc gagccggttc gcttacagca gatagcctgg
     5161 aatcttgttt ctaatgcgat caagtttacc gaatccggcg gcagagttcg cgtgttaact
     5221 tcgactaacg gcatcgatgc aacgttcgtc gttgaggact cgggtattgg tattgaaccc
     5281 gatttcctcc ctcatgtctt cgaccgattt cgtcaagcag atggttcaac ctccagagtt
     5341 catggtggtt tggggttagg actcgctatc gctcatgcgc tcgcgaaaat gcattcgggg
     5401 cagcttgagg cttactcgga gggagttggt aaaggagccc gcttcaccct tcggttaaaa
     5461 ctggctatcg gtgcaaaagt cgatcctgag gttacgacga agcaagagca ctcctttgcg
     5521 ggtttggagg ttctgatagt tgaagattcg ccggacacgc ttactttatt gagcactatc
     5581 ttcagtcagg aaggcgcgaa tgttgccgcg gctacctcag ctaacgatgc actgaatcgt
     5641 attctcgaaa aaagacccga tgtcattatt tctgatattg gtatgccgga cacggacggg
     5701 tacaaatttc tcgaaaaggt tcgagagtta ccagctgcga gacacattcc ggccatagcg
     5761 gtttccggat acgccagtga cgaggatcgg aagcgtgcgg tcagtgttgg ctacgccgcg
     5821 ttaataccta aacctatcga cgtgaatgag ctctttgaaa ccatcaatga gttgggtatc
     5881 ggtgagttgt cgtcgaagga gtaatcaaaa ccgacggctg ttctcccgac gaaaacgaaa
     5941 tcttcaagga aatatcattg tcgggcaggc aagtttcccg gaatccttta gtgttcctca
     6001 tggcatctta gaggcggaat tgatgtgttg ttttgattcc tattcccgca aaacccctag
     6061 tggggagtgc ggtgcggatt gagaagctgt tcattggctt tgaaaatccg tgcgtaaagc
     6121 cctgggagga aagggtttac gtgaatttat caggattctg aatgacaagt taattcgggt
     6181 ttgacaaatt aaaaactgta actataatgg ttacaaaagg gagtagcccc gagaccgcgg
     6241 taagccagat caactatgtc aacaaatagc cgatttgccg tcgcagttca tgtattgagc
     6301 ttgat
//
LOCUS       DASSFP010000418         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1454693, whole genome shotgun sequence.
ACCESSION   DASSFP010000418 DASSFP010000000
VERSION     DASSFP010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454693"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1396)
                     /gene="recA"
                     /locus_tag="VFH91_09425"
     CDS             complement(254..1396)
                     /gene="recA"
                     /locus_tag="VFH91_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET6863258.1"
                     /translation="MCVKLRVRYGEGTYELREKERKKEMYAMADDRMNADRGRAVEAA
                     LSNIEKKFGKGSIMRLGERDVSEVPAISTTTLSLDAAVGVGGVPRGRIVEIYGPESSG
                     KTTLALHIVAEAQKAGGVAAYIDAEHAMDADYAGKLGVDIDQLLISQPDSGEQALEIA
                     EALVRSNGIDVIVIDSVAALVPRAELDGEMGDSLPGLQARLMSQALRKLTAIVAQSNT
                     CFIFINQIREKIGVMFGSPETTTGGRALKFYASLRLDIRRIGAIKDGDRVVGNRTKVK
                     VAKNKVAPPFRECEFDIMYGEGISREGDLLDLAVANRAVEKSGAWFSYKGERLGQGRE
                     NAKQALKDNGELLKRIESEVKVKLGLPGHAEAEPANAKAATATDKK"
     gene            complement(1539..1886)
                     /locus_tag="VFH91_09430"
     CDS             complement(1539..1886)
                     /locus_tag="VFH91_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863259.1"
                     /translation="MSEVNQVPNGFTRGTPVLIVLQNPREKCWGLLDDITTAGVFLRG
                     LDLNAFDEWIHAIVHDEATVGLGSLFFPMWRVEKIARDETLAGIPSLALQVEQRTGRK
                     LEDIVQDQESPFV"
     gene            complement(1883..2683)
                     /gene="trpA"
                     /locus_tag="VFH91_09435"
     CDS             complement(1883..2683)
                     /gene="trpA"
                     /locus_tag="VFH91_09435"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET6863260.1"
                     /translation="MSRISQVFSQLKNEGRKAFVPFITAGDPDLPATGEFLVELARAG
                     AGVIELGVPFSDPMADGPVIQRASERALEHKFGLDEVFQLIEQSRRKIDTPIILFSYF
                     NPLLQYGLSRLCQEAKRVGLDGVLVTDLTPEEATQFSAELKSHDLDMIFLIAPTSTDV
                     RLRLVAKHASGFIYAVSRAGVTGTRESVSVEAEKLVKRMRAFSDLPIVVGFGISSPSQ
                     VAEVERYADGVVVGSAIVAEIERSKGREDIAAHIGQFTRSLIPALNAK"
     gene            complement(2687..>3681)
                     /gene="trpB"
                     /locus_tag="VFH91_09440"
     CDS             complement(2687..>3681)
                     /gene="trpB"
                     /locus_tag="VFH91_09440"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET6863261.1"
                     /translation="ELAGGPKIYLKREDLLHTGAHKINNTIGQVLLARRMNKRRIVAE
                     TGAGQHGVATATVCALFGLDCVVYMGSEDMRRQALNVFRMQLLGAEVRAVDSGSRTLK
                     DAINEALRDWVTNVADTYYLLGSALGPHPYPLMVRDFQSVIGRETRRQLLEIEGRLPD
                     LLVACVGGGSNSIGLFHPFLADSSVRMVGVEAGGSGNELGQHAARFNAAGGGSPGVLQ
                     GTMSYLLQDKNGQVATTHSVSAGLDYPSIGPEHAFLHDSKRVEYVSVTDDEALEAFHL
                     LSKLEGIIPALESSHAVAQSLKLARDMRPDQLVVVNLSGRGDKDVDTVRERLEL"
BASE COUNT          906 a         1035 c          863 g          877 t
ORIGIN      
        1 ttgaagagtg atgtttactt ggcccttggc ctttggcctt tggcccttgg cttttggcct
       61 ttggcctttg gtctttggac gaccacagtc agtaccggat cgcggtagcg ccagcttaac
      121 ttcgagattc tgatagccaa gatcaaaggt caaagagcca aaggccgaaa acctttacca
      181 gtttaacgcc atgaataagc gctcgtcgaa gccttacgga cctcggcgag cgctaaaaag
      241 aagggtggtc taattatttc ttatcagtcg ctgttgcagc ctttgcattt gctggctcag
      301 cttctgcatg gcctggaagt ccgagtttga ccttaacttc agactcgata cgttttagga
      361 gttcgccatt atcttttaaa gcctgcttgg cgttctctcg accctgtcct aaccgttcac
      421 ccttatagga aaaccacgca ccactctttt ccacggcgcg attagcaacg gccagatcga
      481 gcaggtcacc ttcgcgagaa ataccctcac catacatgat gtcaaactcg cactcacgaa
      541 atggcggcgc cactttgttt ttagcgactt taactttggt acggttacca actacccgat
      601 cgccatcttt gatagcgccg attcgccgga tatcgagtcg cagcgatgcg taaaacttca
      661 gtgcgcgtcc cccggtggtt gtttcgggac taccgaacat gacgccgatt ttttcccgga
      721 tctggttgat gaaaataaag caggtattcg actgagcgac gatcgctgtc aatttccgta
      781 gcgcctgtga catcaatcga gcttgcagtc caggtaaaga gtcacccatt tcgccatcga
      841 gttcggcacg cggtactaac gccgcaaccg agtcaatgac gattacatca atcccattgg
      901 atcggacgag cgcctcagca atttcgaggg cttgctcacc ggagtcgggc tgggaaatca
      961 aaagctgatc gatatcgact ccgagtttcc cggcgtaatc ggcatccatg gcatgttcag
     1021 cgtcgatgta ggcagctaca ccgccggcct tttgagcctc agccacaata tgaagagcga
     1081 gagtagtttt tcctgaactt tcaggaccat aaatctcgac gatacgccca cgcgggacac
     1141 cgccaactcc aacagccgca tcgaggctca aggtcgtagt ggagatggct ggcacctctg
     1201 aaacgtcgcg ctctcccaaa cgcatgatag agcctttgcc aaacttcttt tcaatatttg
     1261 acagtgccgc ttcgacagcc ctgccgcgat ctgcattcat tcggtcatca gccatggcat
     1321 acatctcttt cttccgctcc ttttctcgca attcataagt gccttccccg tagcgaacgc
     1381 ggagtttaac acacacgagg cgagaatgga acagtgtttc acgcttgcaa caagattgga
     1441 gggtgaggaa ctggaattag ttttacgcaa aaatgagcag ccctctctgc atcggactca
     1501 taaagtgagg ttgcgtgtta ccaactccca acaatgatct aaacaaatgg gctttcctgg
     1561 tcctgtacaa tatcttcgag ctttcgaccg gttcgctgtt caacctgtaa cgcgagtgaa
     1621 ggaatgcctg ccagagtttc atccctggca atcttctcaa cgcgccacat cggaaagaag
     1681 agcgaaccca atccaaccgt ggcttcatca tgaactatcg catgaatcca ctcgtcaaac
     1741 gcattcaagt cgagtccgcg caagaatact ccagcagtcg taatgtcgtc gagcaacccc
     1801 cagcattttt cacgagggtt ctgcaggacg atgagtacag gcgtacctct cgtaaatcca
     1861 tttggtactt gattcacttc actcatttcg cattcagcgc cggtatcaaa cttctggtga
     1921 actgaccgat atgagccgcg atatcctcac gtcctttgga tcgttctatc tcggcaacta
     1981 tagctgaccc tacgacaaca ccgtcagcgt atcgttcaac ttccgccact tgcgatggag
     2041 aagagattcc aaaaccgacg acgattggta gatcgctaaa ggctcgcatt cgcttgacca
     2101 acttctccgc ttccacgctg acgctctctc gagtgcctgt cacaccggct cgcgacaccg
     2161 catagataaa accgctggcg tgtttcgcca ctaaccgtag tctcacatcg gtcgaggttg
     2221 gagcgataag aaaaatcatg tccaggtcat gtgacttcag ttcggccgaa aactgcgttg
     2281 cctcttcagg agttagatct gttaccagca ctccatcgag tccaacgcgc ttcgcctcct
     2341 ggcatagccg gcttaagcca tactgtaaaa gcggattgaa gtagctaaat agaatgatcg
     2401 gcgtatcaat ctttcgtcgc gactgctcga taagttggaa gacttcgtcg agaccaaact
     2461 tgtgttcgag agcgcgttcc gaggctcgtt gaatcactgg tccatcggcc atcggatcac
     2521 tgaagggaac gcccagttca atgaccccag ctcccgcacg ggccagctcg accaagaatt
     2581 ccccggttgc cggcaaatcc gggtcgccgg ccgtgataaa cggaacaaaa gccttacggc
     2641 cctcattttt caactgtgaa aagacttgcg agattctgct caaagattaa agctccaggc
     2701 gctctcttac cgtatcgaca tccttgtcgc cacgacctga aagatttacg acgaccaact
     2761 gatctggcct catatcccga gcaagtttga gactttgcgc cacggcatgt gacgattcga
     2821 gtgccggaat gatcccttcc aacttagaaa ggagatgaaa cgcttcaagt gcttcatcgt
     2881 cggtaacaga gacatactcg acgcgtttcg aatcatgtaa gaacgcgtgc tcaggaccaa
     2941 tagatggata atcaaggccg gcagagaccg aatgagttgt cgctacttgt ccattcttgt
     3001 cttgcagcaa ataactcatc gtgccttgca aaactcctgg acttcctccg cccgctgcat
     3061 taaatcgcgc tgcatgttgg ccaagctcat tgccggatcc tccagcctct actccaacca
     3121 ttctcacaga agaatccgcg agaaaaggat gaaacaatcc gattgaatta gaaccaccgc
     3181 caacgcacgc gacaagcaaa tcaggtaagc ggccttctat ttccagtaac tgcctgcggg
     3241 tctcacgacc aataacactc tggaagtcac ggaccatcaa tggatacgga tgaggaccca
     3301 gcgcactgcc gagaagatag tatgtatcag cgacattggt aacccaatcg cgtaacgctt
     3361 cgttaatagc atctttcaga gttcgcgagc ctgaatcgac tgcgcgtacc tcagcgccca
     3421 gcaactgcat gcgaaagaca ttcaacgcct ggcgtcgcat gtcttccgag cccatgtaga
     3481 cgacgcagtc caaaccgaac aaagcacaaa cggtggcagt agcgacaccg tgctggccgg
     3541 ctcccgtctc agcaactatt cgtcgcttgt tcatgcgtcg cgccagcaga acttgaccaa
     3601 tggtgttgtt aatcttatga gcgccggtgt gaagcaagtc ctcgcgcttt aagtagatct
     3661 ttgggccacc tgcgagttca g
//
LOCUS       DASSFP010000419         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1157242, whole genome shotgun sequence.
ACCESSION   DASSFP010000419 DASSFP010000000
VERSION     DASSFP010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157242"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFH91_09445"
     CDS             complement(<1..1322)
                     /locus_tag="VFH91_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863262.1"
                     /translation="MKKTIFLALLLSSFLFSLALGQDGSGVGGSTFPKNDITYSTEVV
                     TAHVPWATKLPGGPIKSFFIPSVQYGRDMVELMQRLQLQPTTDSIDSSWDINCWGIGD
                     FYGHEFRGDRDDFQIVYGYVEKDLTGSTPFEVMVIPGLNGWTRMTRATRDAILRRVQE
                     GAGLVLLHPFVGDVEGHPFKGDEGQGDQRIWDISPLVGVPDDTVNERGYPEINRDAIS
                     KGQWQINQSHFITDALPLELLPEGNTGGSFYKYRANGDVLVRSGEYPIVATKNYGRGR
                     VVALGYTEEGFTPQSINPYEARIYWDYWEYQYSLLARSILWASGRQVPIKFKSVTANS
                     SGINLSISSDVKRSVEIEVAGKNEFGQSLGSTRIDRTIDAGVSTLDIPALSLKTKDAW
                     PAGRQIFNLIIRDAESKTTLNWGSATFTTPKQAMMTSVKPAVDVYRRG"
     gene            complement(1589..2554)
                     /locus_tag="VFH91_09450"
     CDS             complement(1589..2554)
                     /locus_tag="VFH91_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6863263.1"
                     /translation="MLRLALVGCGEHSRMSHAAPLAAYAANHPDQIELAAACDLNIDK
                     ASEFCRSFGFARAYTEFERMLDTEKVDACVSVMPVDHITQIGMNLLQRRIPCVIEKPL
                     GISLHEAEHLAAAAAETQTPHMVSVNRRFMPYLNRARSWANEQGSLAYVRATQVRYAR
                     DESDFIWSTAIHVLDATRHIAGEIVNFEAQSWTERDMSSRWFLIFLDFENGAKGTIEV
                     LPTSGMVEESYELFGEGFRARVTAGAGPQRTLDCWREDQLSSHAKASDDEPEFLRNGS
                     YQEVEEFVSALRSGNRPHPEIKDILPSTRISFAIAELFRSDDTQK"
     gene            complement(2564..>2999)
                     /locus_tag="VFH91_09455"
     CDS             complement(2564..>2999)
                     /locus_tag="VFH91_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6863264.1"
                     /translation="LKASPDLVKPNRNEAESFVGHPVGDGLAAIEAAQKFFKANAKSV
                     AISLGPDGMLWQRSEDSAPLISQLPPILGCSSVGCGDASLAGFAVAHERALCEEETIS
                     LAVACGAANCLADAPGQIDPYNVERIAGQVLIQRVHSECQTG"
BASE COUNT          706 a          764 c          769 g          760 t
ORIGIN      
        1 tcgcctcggc gatagacgtc aaccgcgggt ttgacgctcg tcatcattgc ttgtttcggt
       61 gtggtgaatg tggctgagcc ccagttgaga gttgttttcg actcagcatc gcgaataatc
      121 aaattaaaga tctggcgtcc agcgggccag gcatccttcg tttttaaact taaggccggt
      181 atatcgagcg tgctaacacc ggcatcgatt gtgcgatcaa ttctggttga accaagtgat
      241 tgtccaaact cattctttcc ggcgacttca atttctactg agcgcttcac gtcagaagag
      301 atcgaaagat tgattccgga gctgttggca gttacagatt taaatttgat tggcacttgc
      361 cgaccggatg cccacaggat tgatcgcgcc agcaaggaat actgatactc ccaataatcc
      421 caatagattc tcgcttcgta agggttgatg ctttggggag tgaagccctc ttcggtatag
      481 cccagtgcaa ccacacgtcc tcgaccgtaa ttttttgtag cgacgatcgg gtattctccc
      541 gaacgaacca acacatcgcc attcgcccgg tacttataga atgagccacc agtatttcct
      601 tcgggtaaaa gttcaagcgg tagcgcgtca gtaatgaagt gagattgatt gatttgccat
      661 tgacccttgc taatcgcatc gcgattgatt tctggatagc cgcgctcgtt gacggtgtcg
      721 tccggaactc ccacgagtgg cgaaatgtcc cagatacgct ggtcgccctg gccctcatct
      781 cccttaaatg gatgaccttc tacgtctccg acaaaggggt gaagcaaaac gagacctgcc
      841 ccttcctgaa cgcggcgaag aattgcgtct ctcgtagcgc gcgtcattcg cgtccagcca
      901 tttagacctg gtatcaccat cacttcaaac ggagtactgc cagtcagatc tttctcgaca
      961 tagccgtaca caatttgaaa gtcgtctcga tcgcctcgaa attcgtggcc gtagaaatca
     1021 ccgattcccc aacagttaat gtcccagctg ctgtcgatgg aatctgttgt gggttgcagc
     1081 tgaagacgct gcatcaactc gaccatgtct cgtccgtatt gaactgaagg gatgaaaaag
     1141 gactttattg gaccaccggg cagtttagtt gcccatggga cgtgtgcagt tacgacttcc
     1201 gtggaatagg tgatgtcgtt ttttgggaat gtgcttccgc cgactccgga tccatcttgg
     1261 cccaaagcga gggagaacaa gaaggaagaa agaaggagtg ccaggaaaat ggtttttttc
     1321 atgacggtga acgcttcgag tatttggtct gctgtaaccc cttattggtc attggtcatt
     1381 ttcatatgcc acgccgcaaa aggcttcatt ggagaattgt gaatgataat taagaattgc
     1441 tatcttactc tcaccaagct tcagacctgt ggtagcaaag atcagcaccg atgtccgata
     1501 tgctttagct tgtcggtgtc gctcattaca ctaacctcag atgttcatga atcaacgaga
     1561 gcgaacaagc taaagcatat cggacacctc acttctgagt atcatcgctg cgaaatagtt
     1621 ccgcaattgc aaaactgatc cgcgtagatg gaagaatgtc cttaatttcg ggatgggggc
     1681 gattgccgct tcgaagtgcg ctcacaaact cttctacttc ctggtacgag ccgttgcgta
     1741 gaaactcggg ctcatcgtca gaggctttgg catgagaact taattggtct tcccgccaac
     1801 agtctaacgt gcgttgtggt cctgcgccgg ctgttacgcg ggcccggaag ccttcgccaa
     1861 aaagttcata cgactcctcc accatacctg acgttggcag cacttcaatt gtgcctttgg
     1921 cgccattctc gaaatcaaga aagataagga accaacgcga tgacatatct cgctcagtcc
     1981 aactttgagc ttcgaaatta acaatttcgc cggcaatatg acgcgtcgca tcaagcacgt
     2041 ggatcgcagt ggaccaaatg aagtcgcttt catctcgagc gtaccgcacc tgagtggcgc
     2101 gtacatacgc aagcgagcct tgttcattag cccaggaacg tgcgcggtta aggtagggca
     2161 tgaaacggcg gtttaccgac accatgtgtg gtgtttgcgt ctcggcggca gccgctgcca
     2221 gatgttccgc ttcgtggagt gaaattccaa gtggtttttc aatgacgcac ggaatgcgac
     2281 gctgaagtaa gttcattcca atttgcgtga tgtgatccac tggcatgaca ctaacgcagg
     2341 cgtcgacctt ttcggtgtct agcattcgtt cgaattctgt gtaggcccgt gcgaaaccaa
     2401 acgagcggca gaactcggat gccttatcaa tgttcaggtc acaagcagct gcaagttcaa
     2461 tttggtctgg atggtttgcg gcgtaggcag ccaggggggc cgcatggctc atgcgtgaat
     2521 gttcaccgca accgactaat gcgagtcgta gcatagtgat tgctcagcct gtttggcatt
     2581 cagagtgaac tcgttgaatc agtacttgcc cggcaattct ttcaacgttg tacggatcaa
     2641 tctgacccgg cgcgtcagca aggcaattgg ccgcaccgca ggctactgcc aaactgatcg
     2701 tttcctcttc gcagagcgct ctttcatgag cgactgcaaa cccggccagt gatgcatcgc
     2761 cgcagccaac cgatgagcaa ccgaggattg ggggcagttg tgaaatcaat ggagcgctat
     2821 cttccgagcg ttgccagagc ataccgtctg gtccaagcga gatcgcgaca ctctttgcgt
     2881 ttgccttgaa gaatttctga gcagcttcga tggcagcgag gccgtcgccc accggatggc
     2941 ctacgaaaga ttccgcctcg ttgcgattcg gctttacgag atccggcgat gcttttaat
//
LOCUS       DASSFP010000420         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2214398, whole genome shotgun sequence.
ACCESSION   DASSFP010000420 DASSFP010000000
VERSION     DASSFP010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214398"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2069
                     /locus_tag="VFH91_09460"
     CDS             <1..2069
                     /locus_tag="VFH91_09460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6863265.1"
                     /translation="KKPMNHTARLRAFVLVLCVFPSILGQQALRGITPEDYFSFEFLS
                     DPHLSPDGKLAAYVITKVDRAQNRRNSSIWLVATDGTTPPRQFTTSPQSVNYPRWSPD
                     GQSLAFLSSRPGAETTPGEQTRSQVYLLSMSGGEARRLTNLKNGVSGFQWSPDGTQLV
                     VTSRTGPSDNRTESKDRSDVRHYKNSSYKFNDTGWFDDRRSHFWIVDLKTGNAKQLTE
                     GNDWNDSDPQWSPDGARIAFVSNRTGKELDGDRNSDVWVIPATGGSLIKISDHAESDS
                     SPRWSPDGKTIAFTGEIRERDHPKIWLAPAAGGRPSVLAATDLDLIPSALEWSNDGKS
                     IYFESGVKGEYQLFRVNLDKKSTEQISSGARAVRNAEFNQRANLRIFTVNDFKHLDDL
                     YVSDITGNKERKLTNLNDALWKQVQFADVERFTYKSADDWAIDGFLVKPLGWQSGKKY
                     PLILSVHGGPSGQYGVDWYHEFQVYAAKGYAVLFTNPRGSTGYGQKFERGIFGEWGGK
                     DYLDVMNGVDEALKRYPWIDADRMGVTGGSYGGFMTNWIVGHTDRFKAAVTLRSVVNF
                     ISDEGTRDGAYGHADDFGGDLFEKFDLYWDRSPLKYAKNVKTPTLILHSDNDFRVPLE
                     QGEQWFRALRHFGVTTEIVMFPRENHNLTRTGEPRHLVESLNWQLYWFDRFLNGNGSA
                     VPPSLK"
     gene            complement(2072..3226)
                     /locus_tag="VFH91_09465"
     CDS             complement(2072..3226)
                     /locus_tag="VFH91_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET6863266.1"
                     /translation="MTDQAKANPTSSSAAATGLRGVVVGTSTVSDVVGDKGELIYQGY
                     NIHDLARHSNFEEVAFLLWNKRLPKRSELDDLRRQIGKSYAVPSPVIDLLRQLPREAD
                     PIDVLRTAVSALESYDPAARDLSPEASRRTSIKLTAQFATLVAAAERIRRGEEPVAAL
                     PDENIATNFLYMLKGEKPSEHDAHLFDVCLVLHADHELNASTFTARVVAGTLASLYAS
                     VVAAIGALSGPLHGGANTNVMKTLLEIGEVANVESFVKRALAEKRKMMGFGHAVYKTE
                     DPRATHLRQFSKEMGERSGESKWYEMSRKVEEVMMREKGLYPNVDFYSASTYYMMGIP
                     LELYTPIFAVSRITGWTAHVLEQYADNKLIRPRAEYVGQRNVPYVPIDQR"
     gene            complement(3308..4117)
                     /locus_tag="VFH91_09470"
     CDS             complement(3308..4117)
                     /locus_tag="VFH91_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET6863267.1"
                     /translation="MKTGRVGAVTSTLPVISLLTDFGINDYFVGAMKGVIISITDALL
                     VDITHEIPAHDIEAAAFTILAAYQSFPPNAIHLAVVDPGVGSDRRPIVVGTPVGTFVG
                     PDNGIFSYVLEQAERFEAFHITNRKYFREPISNTFHGRDVFAPVAAHLARGVRPKTLG
                     PRILDPVFLPSLQTRKRKADLEGRIIHIDRFGNCVTNFKRADIDKPCQLRLKGRRINT
                     FKQFYAEQPGSRGELFGIWGSAGFLEIAATNMSAAKLLRAKRGDQVKVVRV"
     gene            complement(4135..>5186)
                     /locus_tag="VFH91_09475"
     CDS             complement(4135..>5186)
                     /locus_tag="VFH91_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863268.1"
                     /translation="RKDRMMNYVKFPLMILLSIALIGPAALVSAAVPVQGSTALERGF
                     RTGYSDGYNAGFKDLADNAPRNYQNKEEYQRADRSFNDVWGPIEDYRDGYQQGFEKGY
                     AAGFDHQQFDSSIPGGLRRRQQQQQQPVAAVDNNGTVAPNNYPTANDNTKANDNTKAN
                     DNTTVPVQTGSLSIPRNTILALELLTPLSTDVSQRGDHFQARVTEPAEYAGAIVEGRV
                     SQVKRPGKVKGVAQLQLSFEQIRTTDNRVSSLHAELVEVVPMTPDGPSSVDPEGGVKG
                     RDSTKDDATKIGAATGIGAIIGAIAGGGSGAAIGAIIGGGAGTAGVMTQRGKDIRLDR
                     GQQMKIRTSADTNVQ"
BASE COUNT         1272 a         1311 c         1324 g         1279 t
ORIGIN      
        1 aaaaaaaacc gatgaaccac actgccagac tgagggcgtt cgtactcgtc ctatgcgttt
       61 tcccgtctat tcttggccag caagcgttgc gaggtattac gccagaagat tatttctcct
      121 ttgaatttct cagcgaccct catctttcgc cggatggaaa acttgcagca tacgtaataa
      181 cgaaggttga tcgcgcacaa aatagacgta actcatcgat ctggttggtc gcaactgacg
      241 gaacgacacc tccaaggcaa tttacaacca gccctcaatc cgtgaactac ccacgctgga
      301 gtcctgatgg tcagtcactg gcgtttcttt caagcaggcc tggggcagaa acaacgcccg
      361 gcgaacaaac acgttcgcag gtttatctac tttccatgtc tggcggagaa gcgaggcgac
      421 tgacaaattt gaaaaatgga gtgagcgggt ttcagtggtc ccccgacgga actcagttag
      481 tcgtcacaag tcgaaccgga ccaagtgaca atcgcacgga gagcaaagac cgcagcgatg
      541 tgcgacatta caaaaacagc tcttataaat ttaacgatac tggctggttc gatgatcgac
      601 gcagccactt ctggattgtc gatcttaaaa cgggcaatgc taaacaattg actgaaggta
      661 acgactggaa cgatagcgat ccgcagtggt ctcctgacgg tgctcgcatc gccttcgtat
      721 caaatcgaac agggaaagag cttgacggag accgcaactc tgatgtctgg gtcattccgg
      781 cgacgggtgg gtcgttgatc aaaatctccg atcacgctga aagcgacagc agtcctcgat
      841 ggtcgcctga cgggaaaacc atcgctttca cgggagagat ccgtgaacgc gatcatccga
      901 agatttggct ggcgcctgcg gcaggcggcc ggccatcagt gctggccgca acagatctcg
      961 accttattcc gtctgctctt gagtggtcca acgatggtaa atcaatctac ttcgagagcg
     1021 gcgtgaaagg tgaataccaa ctctttcgcg taaaccttga taagaaatcg accgaacaga
     1081 ttagttccgg agccagagca gttcgcaacg ccgaatttaa ccaacgtgct aacctgcgaa
     1141 tctttaccgt taacgacttc aaacatctcg acgatcttta tgtctcagat attactggta
     1201 acaaagagcg caaactaaca aatttgaatg atgcgctttg gaaacaggtg cagtttgccg
     1261 acgttgagcg cttcacctac aaaagcgcgg atgattgggc catcgatggc ttcctggtca
     1321 aaccgttggg ctggcaatcg ggcaagaagt acccattgat tctaagcgtc catggcggac
     1381 cttcgggcca gtatggagtt gactggtacc acgagttcca ggtttatgca gctaaaggtt
     1441 acgcggtttt gttcacgaat ccgcgtggct caactggata cgggcaaaag tttgagcgag
     1501 ggatctttgg cgagtgggga ggcaaagact atctcgatgt gatgaatggt gttgacgaag
     1561 ctctgaagcg ttatccatgg attgacgctg atcgaatggg agtcacaggc gggagttacg
     1621 gcggtttcat gaccaactgg atcgtagggc acactgatcg cttcaaagca gctgtaactc
     1681 tccgaagtgt cgtgaatttt atttcagatg aaggaacccg tgacggtgct tacggccatg
     1741 cagacgattt cggtggggac ctttttgaga agttcgatct ctattgggac cggtcgcctc
     1801 tcaaatacgc taagaacgtc aaaacaccga cgctgattct tcactccgat aatgatttta
     1861 gagtacccct tgaacaaggc gagcagtggt ttcgggcgct caggcacttc ggggtcacaa
     1921 cggaaattgt gatgttcccc cgtgaaaacc ataatttaac tcgtacaggc gagcccaggc
     1981 atctggttga aagccttaac tggcagctct actggtttga tcggtttttg aatggtaacg
     2041 ggagcgccgt gccgccaagt ttgaagtaac ctcagcgttg atcgatcgga acataaggta
     2101 cgttcctctg gccgacatat tccgcgcgcg gtcgtataag tttgttgtcc gcgtattgct
     2161 cgagaacgtg ggcggtccaa ccagtgatgc gactgacggc gaaaatcggc gtatacaact
     2221 ccagcgggat tcccatcatg tagtaagtcg aggctgaata aaagtctacg ttcggataga
     2281 gacccttctc gcgcatcatt acttcttcaa ctttgcggga catttcgtac cactttgact
     2341 cacctgaacg ttcacccatt tctttggaga attgacgcag atgtgtcgcg cgcggatctt
     2401 cagtcttgta tacagcgtga ccaaaaccca tcatctttcg cttttccgcg agagcgcgct
     2461 tcacaaaact ttctacattt gccacttcgc cgatctcgag caaagtcttc atgacgttgg
     2521 tattagcgcc gccatgaagt ggaccagaaa gtgctccgat cgccgccacc accgaggcat
     2581 acaggcttgc taaagtcccc gccacaacgc gagctgtaaa cgtcgaagca tttagttcgt
     2641 gatcagcgtg gagcacaagg catacatcga agaggtgagc atcgtgttcc gaaggctttt
     2701 cgccttttag catgtaaaga aaattggtcg cgatgttctc atcaggcaga gcagctactg
     2761 gttcctcacc tcgtcttatt cgttctgccg cggccacaag agttgcaaac tgggccgtca
     2821 acttaatcga ggtccggcga ctcgcttccg gagataggtc gcgtgcagct ggatcgtatg
     2881 actcaagcgc cgacaccgcg gtccgaagca cgtcgatggg gtcagcctct cgaggcaatt
     2941 gacgaagaag atcgataacc gggctcggaa ccgcgtaaga tttgccaatt tgtcgtcgca
     3001 agtcgtcgag ctcagagcgc ttcggcagcc gtttgttcca gagtaaaaaa gcgacctctt
     3061 caaagtttga atgcctggca aggtcgtgaa tgttataacc ctgatagatc agttcgcctt
     3121 tgtcgccgac gacatctgaa acagtggagg tgccaactac gacgccgcgt aatccggttg
     3181 cggcggcgct tgaagaagtg ggattcgctt tcgcttggtc agtcataaat ttttttgttt
     3241 tcagccgaaa gataacaaag aggtgacaca gtgacaagcg gcgatgatct ttagcaaaca
     3301 ccaggactta aactcttaca actttgacct ggtcgccgcg tttggcacga agcaacttag
     3361 ctgccgacat gttggtcgct gcgatctcta aaaacccagc actaccccag atcccaaaga
     3421 gctcacctcg cgaaccaggc tgctcagcat agaactgctt gaacgtattg attcttctgc
     3481 ccttcaagcg taattggcag ggtttgtcta tatcagcacg tttgaaattc gtaacacagt
     3541 ttccaaatcg atctatgtgg ataattctgc cttcgagatc tgcttttctt ttccgcgttt
     3601 gaagactggg gagaaaaacc gggtcgagaa ttcttggtcc taaagtcttt ggtctcacgc
     3661 ctctcgctag gtgagctgct acaggtgcaa atacgtcgcg cccgtggaag gtgttgctga
     3721 ttggttctcg aaaatactta cggtttgtaa tatgaaatgc ttcgaacctc tctgcttgtt
     3781 ctaatacata actaaaaatc ccattatcgg gtccaacaaa ggttcctacc ggtgttccaa
     3841 caactattgg ccttcgatcc gaacctacac ccggatcaac aactgccagg tgaatcgcgt
     3901 ttggcgggaa ggattgatat gcggcaagga tggtgaatgc ggccgcttct atgtcgtgag
     3961 cagggatctc gtgagtgata tctaccagta gagcatcagt gattgagatg atgacgcctt
     4021 tcattgcgcc gacaaaatag tcgttaatac caaaatctgt gagtaaagaa ataacgggaa
     4081 gagttgacgt tactgcgcca actcttcccg tcttcaactt cctaccattt gcgatcactg
     4141 aacgtttgta tcggctgaag tgcgaatctt catctgctga ccgcggtcca accttatgtc
     4201 cttgccgcgt tgagtcataa ccccggcggt tccggcaccg cctccgatga tagctccgat
     4261 cgcggcgcca cttcccccac cagcgatggc gcctatgatt gctccgattc ctgtagcggc
     4321 gccaatcttg gtagcatcat ctttggtaga gtcacggcct ttaactccgc cttcgggatc
     4381 cacactcgat ggaccatcag gagtcatcgg aacgacttcg acgagttcag catggaggct
     4441 ggaaactcga ttatcggtcg tgcgaatttg ttcgaatgat aattgtagtt gtgcaacgcc
     4501 cttcaccttt ccggggcgct taacctgcga gacgcgaccc tcgacgatcg cacctgcgta
     4561 ttcagccggc tcagttactc gagcttgaaa gtgatcgccg cgttgcgaga catcagttga
     4621 aagaggggtc agcaactcca gagcgaggat tgtatttctc gggattgaca acgaacccgt
     4681 ttgaacagga acagtggtgt tgtcgttagc tttcgtgttg tcgttagcct tcgtgttgtc
     4741 gttagcggtg ggatagttat tcggcgcgac agtaccatta ttgtcgacgg ccgcgactgg
     4801 ttgttgttgc tgctgctgac gtcttctcaa cccgccagga attgaagaat caaactgttg
     4861 atgatcaaaa ccggcagcat agcctttctc gaaaccctgc tgatatccgt ctcgataatc
     4921 ttcgataggc ccccaaacat cgttgaaaga tcgatccgcg cgctgatatt cctctttatt
     4981 ctgatagttg cgaggcgcgt tgtcggctag atccttaaat ccggcgttgt aaccatccga
     5041 gtaaccagtg cgaaagccac gttcaagagc cgtagatcct tgaaccggta cggcagccga
     5101 caccagtgcg gctggtccaa tcagcgctat ggacaacaaa atcatcaggg gaaatttcac
     5161 gtaattcatc attctgtcct tccgaa
//
LOCUS       DASSFP010000421         8309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_867932, whole genome shotgun sequence.
ACCESSION   DASSFP010000421 DASSFP010000000
VERSION     DASSFP010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867932"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFH91_09480"
     CDS             <1..577
                     /locus_tag="VFH91_09480"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET6863269.1"
                     /translation="FAISEGVTVIDFAGPWEVFQDVMIEQRGSDHEDQMPFQLFTVSE
                     KTDVLTGSAGLKLVPDFTFDNVPQPKVVVIPAQRGSQALHAWLKKVTEKTDVTMSVCT
                     GAFQLGRAGLLSGKAATTHHDFFDQFAKAFPDVTLKRGRRFVENEKISTAGGLTSGID
                     LALRVVERYFGRSVAETTATYMEYQSKGWIV"
     gene            710..2563
                     /locus_tag="VFH91_09485"
     CDS             710..2563
                     /locus_tag="VFH91_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6863270.1"
                     /translation="MRKQFVRIIAVGIVGTSLVIGSTLTFAQQKTRVTKAKRDVVTKA
                     GLPDDSILERYQQSISAERLAATLFFLASDSFEGRETTTRGQKLAALYLASQYGQLGL
                     TPFGPKKVEGPMALDVFFQPFTVYRRTAQQSQLELLDGERVEISSNYSAAKQDDLSYF
                     LTGGLTDAAEGNVVFAGYGISDEKLAYDDYAELAAKKLSLNGKWVLILEDEPMADAST
                     SLLKTADHKPSRWSTQFINKRSVMWAAGQPKGVLVVTGISPRAAGSFSERAQQAALNA
                     QRVGVLSLTDTSAFPPVFAVSPKFADSLFRTSGQTVDSLRKQINQTLKPVVFDLPQNL
                     KLRSSVTPYEGLKTENVIAYLEGSDPKLKDEVLIISAHFDHLGLNPALKGDQIFNGAA
                     DDGSGTVACVELARAFTKAKQEGNGPRRSILFVNFSGEEKGLLGSSYYSRHPIVPWEK
                     TVADINMDGVASFDQGHALNRNYIYSLGTEDLSRQLLDITKQVNDRYKTSLTLVEGQR
                     FNSDQYNFETQLIPYIYFSTGLTEHYHQVSDAPETIDYTHFAKVVQLIFATAWQVANR
                     DTRPPSVSRNQLALVGYTCPSCPFECDDTVYTQPGECPVCGMNLVPKYKTP"
     gene            2635..3753
                     /locus_tag="VFH91_09490"
     CDS             2635..3753
                     /locus_tag="VFH91_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6863271.1"
                     /translation="MTNDPDFQPARNPLHQWLIILGLWTLLGVLYAGPVYFEVRAEGM
                     GHSAWKIFTWGVLTWLVWAPLTPVLMWLARRFSLIGETWKRSLAVHIPAFLLSSAVHS
                     AAATAITLIIKPFDNMGESPSGFWLRYLARLKGSFVPDLMVYSVVVGSCYAIDYYRKY
                     REREFLAAQLETQLAQAQLDTLRMQLHPHFLFNTLNGIVGLVRDNRNDAAVNMLVGLS
                     DLLRHTLEHSDSQEIELKEELQFINLYLRIQQMRFSDRLQIKMDIDPATTRALVPNLL
                     LQPIVENALRHGISRSEANGELTIKSKAENGELRMTVLDNGKGLPANWQLKSSNGIGL
                     ANTVARLQQLYGELHRLDIRNRDQGGVEVTVIMPLRTA"
     gene            3832..4626
                     /locus_tag="VFH91_09495"
     CDS             3832..4626
                     /locus_tag="VFH91_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET6863272.1"
                     /translation="MSQKIRTLIVDDEPLARRNLRLLLEKDPQIEILEDCRNGREAVK
                     SIEQLAPDLVFLDIQMPEMDGFDVLEQVDPNALSAVVFVTAFDQYALKAFEVHALDYL
                     LKPYDDIRFSQALQRAKSQIESREINKVSKRLLALLEERESLRDVTSRKPAYLKRLMV
                     KLANRVVLLKVDEIDWIEADGNYAKLHIGPKSHLLREKMHDLEKQLDPDRFVRIHRSI
                     IVNLNRIKELQPHFNGDYIVVLDNGRQLRLSRSRRDQLESKLRVVN"
     gene            4686..5234
                     /locus_tag="VFH91_09500"
     CDS             4686..5234
                     /locus_tag="VFH91_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863273.1"
                     /translation="MKHLSMPELEAGLDHIRQSPKDQGSLELIVCRPETEQREILDEA
                     QLDVEKGLIGDNWTLRKSTRTSDGSPHPDMQINIMNVRAVALVAQERERWPLAGDQLY
                     LDLDLSKNNLPAGARISIGTAILEVTPPPHTGCQKFVARFGLDAMKFVNSEIGLSLCL
                     RGINAKVVQSGVVRVGQTVKKL"
     gene            5388..6365
                     /locus_tag="VFH91_09505"
     CDS             5388..6365
                     /locus_tag="VFH91_09505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6863274.1"
                     /translation="MEYVNLGKTGLKVSRICLGCMTYGVPERGTHPWSLVEEDSRPLI
                     RQAVELGINFFDTANTYSDGTSEEIVGRALRDFARREELVVATKVFAPMRPGPNAKGL
                     SRKAIMTEVDASLKRLGMEYVDLYQIHRWDDTTPIEETIEALHDVVKAGKALYVGASS
                     MYAWQFARSLYKAEQHGWTRFVSMQNHYNLLYREEEREMMSLCAAEGIGVIPWSPLAR
                     GRLTRDWGVSTNRSETDEFGKTLYTAAVESDKRIVARVAEISAARGVPRAQVALAWLL
                     SKPFLTAPIIGAAKPQHLEDAVAAVSVKLSDEEIKRLEEPYVPHPVVGF"
     gene            complement(6377..7921)
                     /locus_tag="VFH91_09510"
     CDS             complement(6377..7921)
                     /locus_tag="VFH91_09510"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HET6863275.1"
                     /translation="MRSLQAAKVFLLALVCLSAIVVASVSLARQQSPDNTLTGNWAIY
                     SPSTDGYVRTSYFNLKQEGDKISGTIRTTQFFYTIKESSGGPQGFTLFASMMDGKSER
                     KVQYEGKLIGNELQLGRKNRPDQPVTFQTAQRVPDGQGKLPARIDPPARHTVPYNGLA
                     KTPPMGWNSWNKFAGRIDDATVRGIADAMARNGMKEAGYVYINIDDTWEGDRDASGNI
                     QTNKKFPDMKGLADYVHKKGLKLGIYSSPGPNTCAGYEGSYGHEEQDAKTYAAWGIDY
                     LKYDWCGARTLYTDEEMPAIYQKMGDALQKTGRPIVYSLCQYGRFDVWKWGPEVGGNL
                     WRTTGDIRDTWDSMTGIGFKQNDLALYAKPGHWNDPDMLEIGNGGMTDIEYKTHLSLW
                     AVLAAPLLAGNDLRDMSQATLDILTNQEVIAVNQDRLGKQGSQTWKSGEQEIWTRPLT
                     GKATAVAIFNRSSQESRVTVKWSDLKIAKVNSIRDLWSHQDVTALGPEFSTTVPGHGV
                     VMLKVK"
BASE COUNT         2017 a         2006 c         2203 g         2083 t
ORIGIN      
        1 gttcgccatc tctgaaggcg ttacggtaat cgacttcgct ggaccatggg aagtctttca
       61 ggacgtgatg attgagcaac gcggttcaga tcatgaagac cagatgccgt tccagctctt
      121 cactgtgtcc gagaagactg atgtgctaac gggctctgcc ggactgaagc tcgttcctga
      181 ttttaccttt gataatgtgc cccagcctaa agtcgtggtg atacccgcac aacgaggttc
      241 gcaggcgttg cacgcatggc taaagaaggt taccgagaaa accgatgtca ccatgtcagt
      301 ttgcaccgga gcgtttcaac tcggacgcgc aggactactt tctggaaagg cggcgaccac
      361 gcatcacgat ttctttgacc aatttgcaaa agcttttccg gacgtcactc tcaagcgagg
      421 gcggcgattc gtcgaaaacg aaaaaatctc aacggcgggc ggtctgacgt ccggcatcga
      481 tctcgcccta cgcgttgtgg aacgttactt cggtcgttca gttgctgaga ccaccgcaac
      541 ttatatggag tatcagagca agggctggat tgtttaaaga cgtcagggct cagaggtcag
      601 gggtcggtaa atcatttgaa ttatcaattg agaattatga attgataatt atcaaatgat
      661 ttagcgcctt cctgacctct gacccctgac cctcgccatt aggagcttta tgagaaaaca
      721 attcgtacgc atcatcgcgg ttggaatcgt cggaacatcg ttggtgattg ggtcgacctt
      781 gacttttgcc cagcagaaaa ctcgggtaac gaaggcaaaa agagatgtgg tgactaaggc
      841 gggtcttcca gatgactcaa tcctggagcg ttaccagcag tcgatatcag ccgagcgact
      901 cgcggccacc ctcttctttc ttgcctctga ctctttcgaa ggtcgcgaga ccacaacgcg
      961 cggacaaaaa cttgcagcgc tatatctcgc atcacaatat gggcaactcg gactaacccc
     1021 ttttggacca aagaaggttg aggggccaat ggcgctggat gttttcttcc agcctttcac
     1081 tgtctatcga cgtactgccc aacagtcgca actcgaactt ctcgatggcg aacgtgtcga
     1141 aatctcaagt aactactcag cggcaaagca ggatgatttg tcctactttc tcactggagg
     1201 tttaaccgat gcggctgaag gtaatgttgt ttttgccggt tacgggatct ccgatgaaaa
     1261 actggcatat gatgattacg cggagctcgc cgcaaagaag ctgtcgctga atggtaagtg
     1321 ggtgcttata ctcgaagacg agccgatggc tgacgcgagt acaagcctgc tcaaaactgc
     1381 cgaccacaaa ccttcaagat ggagcaccca attcatcaac aagcgcagtg tgatgtgggc
     1441 cgcgggccaa ccgaaaggtg tcctcgtcgt tacaggcatt agtcctcgag cagccgggtc
     1501 gttttccgaa agggctcaac aggcggcact taatgctcaa cgtgtgggag ttctttcgtt
     1561 aactgataca agcgcctttc ccccggtgtt cgcagtctcg ccaaagttcg ctgatagttt
     1621 gtttcgaacg tcgggccaaa cggtcgacag cttacgaaag cagatcaacc agactctaaa
     1681 accagtcgtt ttcgatctcc cacaaaacct taaattacga agctcagtta ctccatatga
     1741 gggacttaaa acagaaaacg tcattgcgta cctggaagga agtgatccaa aacttaaaga
     1801 tgaggtcttg atcatttcag ctcacttcga tcatctcgga ctcaacccgg ccctgaaagg
     1861 cgatcagata tttaacggcg ccgcagatga tggttccgga accgtcgcat gcgttgaatt
     1921 ggcccgggcg ttcactaagg ccaagcagga aggcaatggt ccaagacgct cgatattgtt
     1981 tgtgaatttt tctggtgagg agaaaggcct tcttggctca agttactaca gccggcaccc
     2041 gatcgttccc tgggagaaaa cagttgccga catcaacatg gacggcgttg cgagctttga
     2101 ccagggtcac gcattgaaca gaaattacat ttacagtctg ggaactgaag acctgtcgcg
     2161 gcagttgctc gacatcacga aacaggtgaa cgaccgatac aagacaagcc tgacactcgt
     2221 tgaagggcaa cggttcaact ctgatcaata taactttgaa acccagctca ttccgtacat
     2281 ctacttttca actggtttga ccgagcacta tcatcaagta tcggacgcgc cggagacgat
     2341 tgattacaca cactttgcga aagtagttca actaatcttt gcgacagcgt ggcaagttgc
     2401 aaatagagac acccggccgc ccagtgtgtc acgtaatcag ttggcgttgg tgggctacac
     2461 ttgtccgtcc tgtccgttcg aatgtgatga caccgtgtac actcaaccag gagagtgccc
     2521 ggtgtgcgga atgaatcttg tacctaagta caaaacccct taagagtctt tgtcttagat
     2581 cttttctatt aacatccgtg ctaaaagatg agaatcacac atggaaaatg atcgatgaca
     2641 aatgaccccg atttccagcc ggcaaggaat ccactacacc agtggctgat catccttggt
     2701 ctgtggacgc ttctcggagt cctttacgcc gggccggtct acttcgaagt ccgagccgag
     2761 ggaatgggcc actccgcgtg gaagattttc acatggggag tgctgacgtg gttggtttgg
     2821 gcgccgttga ctccagttct gatgtggctg gctcgccggt tctcacttat cggcgaaacc
     2881 tggaagcgta gcctcgcagt tcacatcccc gcatttcttc tttcctcagc ggtccatagt
     2941 gctgcggcga cggcaatcac cctgatcatt aaacccttcg acaatatggg tgaaagccct
     3001 tccggattct ggctgcgtta ccttgccagg ttgaaaggat cttttgtacc cgatttgatg
     3061 gtttacagtg tggtcgtggg tagttgttac gcgatcgact attaccgaaa gtatcgcgaa
     3121 cgagagtttc tcgcagcgca gctcgaaaca cagctggccc aggcccaact cgatacgcta
     3181 cgaatgcaat tgcatccgca ttttttgttc aatacgctta acggaattgt tggactagtg
     3241 cgggacaata ggaatgatgc cgctgtaaac atgctggtgg gcctcagcga tctattgcgt
     3301 cacacgctcg aacattcgga cagccaggag atcgagctga aagaagagtt gcaattcatt
     3361 aatctgtatt tgcgaattca gcagatgcgg ttttctgatc gattacagat caagatggac
     3421 atcgatccgg ctacaaccag agctttggtg ccaaatcttc tgcttcagcc aattgtcgaa
     3481 aatgctttgc gacatgggat aagtcgcagc gaggctaacg gagagttaac catcaaatca
     3541 aaagccgaga atggcgaact aagaatgacg gttttggata atggcaaggg tttgcctgcg
     3601 aactggcagt tgaagagtag taatggaatc ggattggcaa atactgtagc gcgtttacaa
     3661 caactttatg gagaactgca tcggctcgat attcgcaatc gagatcaggg cggcgtggaa
     3721 gttaccgtga ttatgccact tcggacagct taagatgagt gacgtagcgg ttctcaaata
     3781 gaggaattga tcccgtgaga ggggcgacag ggcgtgcctc ccccgcaaaa gatgagtcaa
     3841 aagatcagaa cgttgattgt ggatgacgaa cctctggcga ggcggaatct tcgtctgcta
     3901 ctcgaaaaag atccgcagat cgagatcctc gaagattgtc gtaacggaag ggaggctgtt
     3961 aagtcaatcg aacaactcgc tcctgatcta gtctttctcg acattcaaat gccggagatg
     4021 gatgggttcg acgttctgga gcaggttgat ccaaatgcac tgtcagcggt cgtttttgtt
     4081 acggcctttg atcaatatgc tttgaaagcg tttgaggtac atgcgctcga ttatctattg
     4141 aagccttacg acgacattcg gttttcgcag gccctacaac gcgcgaagag tcaaattgaa
     4201 tctcgcgaga tcaataaagt cagcaaacgt ttattggcgc tgctggagga gcgcgagagc
     4261 cttcgcgatg ttaccagtcg aaagcctgcc tatctaaaac gtttgatggt gaagctggcg
     4321 aatcgagtgg tgcttttaaa ggttgatgag attgactgga ttgaggctga tggtaactac
     4381 gccaagttgc atattggacc aaagtcacat ttgttgcgcg agaagatgca cgacctggaa
     4441 aagcagctgg atcctgacag gtttgtgcgg attcaccgtt cgataattgt gaacctcaac
     4501 cggatcaaag agctccagcc gcattttaac ggtgactata ttgtcgtgtt ggataatggt
     4561 cgccaactga ggctcagtcg cagccgccgt gatcaactcg aaagcaaact ccgggtggtt
     4621 aattgagaaa aataggccta taagtccaat aggtcctata taattccttt gattggagaa
     4681 ttcttgtgaa gcatctttca atgcctgaac ttgaagctgg attggaccac atccggcaat
     4741 cacctaaaga tcagggatcg ctcgagttga ttgtttgccg gccagaaacc gagcagcggg
     4801 agatcctgga tgaggcccaa cttgatgttg aaaagggact catcggcgat aactggactc
     4861 tgcgaaaaag cacgcgaaca agtgatggtt cgccccatcc tgatatgcag atcaacatta
     4921 tgaacgttcg cgctgttgcc ctggttgcac aggagagaga acgctggcct ttagcaggcg
     4981 accagcttta cctggatctg gatttgagta agaacaattt accagccggc gccaggattt
     5041 caattggtac ggcaattctt gaagtcactc ccccgccgca tactggatgc caaaagtttg
     5101 ttgctcgctt cggtctcgac gcgatgaagt ttgttaattc agagatcggt ctatcacttt
     5161 gcctgcgtgg catcaatgcg aaggtggtcc agtcaggcgt ggttcgcgtt ggacaaacgg
     5221 tgaagaaact ctgatccctc tccgcggctc tctgcgttgt ctctgcgtct ctctgcgatc
     5281 ctccgttggg ccgacggttt atcttcagca gcgacgcgga gatccgcgga gagtcgcaga
     5341 gagacgctga gaggggcaga gaggttgtga gttaaggcgg gataaaaatg gaatatgtaa
     5401 atcttggaaa aacaggactg aaggtatctc ggatttgttt gggatgcatg acgtacgggg
     5461 ttcctgaacg aggcacgcat ccatggtcgt tggtcgagga agacagtcgt cctttgataa
     5521 ggcaggccgt cgaactaggg attaacttct tcgatactgc aaacacctac tcggacggaa
     5581 caagcgagga gattgtcggc cgggcgctac gcgattttgc acgtcgtgaa gagctggtgg
     5641 ttgcgacaaa agtctttgca cctatgcgac ctggtccaaa cgcaaaagga ctgtcgcgca
     5701 aggcaattat gactgaggtc gatgccagtt tgaaaagact gggaatggaa tacgtagatc
     5761 tttatcagat tcatcgttgg gatgatacga ctccgataga agaaacgatc gaggccttgc
     5821 atgatgtagt aaaagcagga aaagcgcttt atgtcggcgc ttcatcgatg tatgcctggc
     5881 agtttgcgag atcgctctat aaagcggagc aacacgggtg gaccagattt gtctctatgc
     5941 agaatcacta caacttgctc tatcgagaag aggagcgaga gatgatgtcg ttgtgtgccg
     6001 ctgaaggtat tggcgtaatc ccctggagcc cgctggctcg cgggcgtctg acacgtgatt
     6061 ggggagttag tactaatcgc agcgaaacgg atgagttcgg aaagacactt tatacagctg
     6121 ccgttgaatc ggataaaaga atcgtggcgc gagttgctga gatctcggcg gcgcgtggcg
     6181 ttcctcgagc gcaggttgca cttgcgtggc tcttgagtaa gccatttctg acagcaccga
     6241 taattggggc tgcaaaacct cagcatctcg aggacgcggt ggcggcggtt tcagttaagt
     6301 tgtcggacga ggagattaag aggctggaag aaccttacgt gccacacccc gtcgtcgggt
     6361 tctagtggag tgattgtcat ttaaccttca acatcacaac tccgtgacct ggaaccgtcg
     6421 tcgaaaattc tggaccaagc gcagtaacgt cttgatggga ccagagatcc cttattgaat
     6481 taacctttgc aatcttgagg tcagaccatt tcacagtcac cctggattct tgagacgagc
     6541 gattaaaaat cgccaccgcc gtagctttcc cggtcaacgg tcgcgtccaa atttcctgtt
     6601 ctcctgattt ccaggtttgg ctgccttgtt ttcccagacg gtcctggttg actgcgatca
     6661 cttcctggtt tgtgagtatg tcgagagtgg cttgcgacat gtcgcgtaga tcgtttcctg
     6721 ccagaagggg agcagcgagc acagcccaaa ggctcaggtg agttttgtat tcgatatcgg
     6781 tcattccgcc gttgccgatc tcaagcatat cagggtcgtt ccagtgtccc ggctttgcat
     6841 acagcgcaag gtcgttttgt ttgaacccaa tgccagtcat ggaatcccac gtgtcacgaa
     6901 tatcacctgt ggtgcgccag aggttgccac cgacttccgg accccatttc cagacatcga
     6961 atcttccgta ttgacacagg ctatagacga tgggccgtcc agtcttttgt aatgcatcgc
     7021 ccatcttttg atagatggcg ggcatttctt catcagtata gagcgtacgc gcgccgcacc
     7081 agtcgtattt caaatagtcg atgccccacg ctgcgtaagt cttcgcatcc tgttcctcat
     7141 ggccataact cccttcatag ccggcgcagg tgtttggtcc aggagaggag taaataccga
     7201 gtttcaatcc tttcttatga acatagtcgg ccagaccttt catgtcagga aactttttgt
     7261 tggtctgaat gttcccgctg gcgtcccggt cgccttccca cgtgtcgtcg atattgatgt
     7321 agacgtagcc tgcttctttc ataccgttac gcgccattgc gtcagcgatg cctcgcacgg
     7381 ttgcatcgtc aatgcgaccc gcaaacttgt tccaggagtt ccaacccata ggtggagttt
     7441 tggccagccc gttgtaagga accgtatgtc ttgctggagg gtcaatacga gcgggtagtt
     7501 tgccttgtcc gtcgggcaca cgttgggcgg tttgaaatgt taccggctga tccggccgat
     7561 tctttctacc aagttggagc tcattaccga tcagtttgcc ttcgtactgg accttacgtt
     7621 cactctttcc gtccatcatt gaagcgaaga gtgtgaagcc ttgtgggccg ccggagctct
     7681 ccttaatcgt gtagaagaat tgtgtggtcc ggattgttcc cgagatcttg tcgccctcct
     7741 gtttcagatt gaagtaactc gtccgcacgt aaccgtcagt gctcgggctg tagatggccc
     7801 aattaccagt caatgtgttg tcaggacttt gttgacgagc cagtgagact gaggcgacga
     7861 caattgctga aaggcagacg agtgcgagga gaaagacttt cgcagcttgc aggcttctca
     7921 tgaaatgacc tccgcaggat ttacagtgag aacgctagaa caggttcttt ctagcacaaa
     7981 aactggaatt tgaaattggt ttattggttc ccgtgaggtg aaccttttgg agtgcgccgg
     8041 cccggcggcg cttttaatgc ctcctgtcct ttgtgctcag agtttcaaat agtcttcttg
     8101 cggctttttg taaccagggc gcgtaaagga ccaaaagcat gaaaagcgcc gccgggccgg
     8161 cgcactccaa agctaggcgg attgtccttt gtgtttagct tctgcgaact cttcaatcat
     8221 tttctgattg aacgccggaa tgtcgtcggg ttttcgactc gttactaacc cgttgtcaac
     8281 cacgacctcg cgatctaccc aatctgcac
//
LOCUS       DASSFP010000422         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1315440, whole genome shotgun sequence.
ACCESSION   DASSFP010000422 DASSFP010000000
VERSION     DASSFP010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315440"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFH91_09515"
     CDS             complement(<1..606)
                     /locus_tag="VFH91_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863276.1"
                     /translation="MDTPDRNLDRPLKAEVNLYYDFKPAAQSKSPLLIALHGYGANKR
                     QMMREAQQMAPQNFAIASLQGFHQHIRDPKEPGGPLRFGFGWLTNFRSEESVAVHHRA
                     LLDLITALVAGGQVDSTRIFLLGFSQSCALNYRFAFTHPEQLRGVIGICGGIPGDWDS
                     SDLYQSANFAVFHLAGAHDEFYPPERCQNYEVQLGTRARDVR"
     gene            818..1135
                     /locus_tag="VFH91_09520"
     CDS             818..1135
                     /locus_tag="VFH91_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET6863277.1"
                     /translation="MKFPGGFNIQQMMKQAQVMQEKMAKEMEELRVDASSGGGVVTVQ
                     MKGNHEILALKIDPEALKDGDAEMLQDLIVAAVNEANRKIDEAMKGKLGGMLPPGLGG
                     LFG"
     gene            1242..1856
                     /gene="recR"
                     /locus_tag="VFH91_09525"
     CDS             1242..1856
                     /gene="recR"
                     /locus_tag="VFH91_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET6863278.1"
                     /translation="MLEYAEPVTKLIDELKRLPSIGHKSAQRLAFHIMRTPESDLERL
                     IAALRDVKQRIVHCSVCNNLTEVNPCRICSSPSRDHSVICIVEEPYSLVAVEKTRSFK
                     GVYHVLHGSLSPIRHIGPDELQLANLLPRFKPENNDGVAVREVILATNPTTEGEATAN
                     YIGRLLKPLGLRVTRIAMGMPVGSDLEYVDEVTMDKALANRHEI"
     gene            complement(1865..2473)
                     /locus_tag="VFH91_09530"
     CDS             complement(1865..2473)
                     /locus_tag="VFH91_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET6863279.1"
                     /translation="MVELIPSPDKIMQILQRTGAFREGHFVYPNGKHSPHYFQMPLAF
                     RYYDTARVLAVALSRKFRLEKDISSQLPKVSVISPSSGGIPVAFGVRDALNAEQIYWA
                     EQEQGKRMFRQYVNQGEVHPCIIVDDIIRSGNAIEETVTLVKSLGARIIGCGAIVRFD
                     SAPKEVEGVPIESLVDYDVKMYESHEPCKECRDNVTSEQVRF"
     gene            complement(2562..3035)
                     /locus_tag="VFH91_09535"
     CDS             complement(2562..3035)
                     /locus_tag="VFH91_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6863280.1"
                     /translation="MIQKLLGKLWRYTPARLRRLTMRMTHARFTVTAGAVIQNEEGKV
                     LLLKHTFRTGSGWGLPGGFLEGGEQPLEGLRRELREEIGLEIERVEFFTARSFKKPQQ
                     VEILFHAFSSGDAKQKSIEIDRATWFSPDSLPEGLPKDQRLIVQRALGDRGKAQT"
     gene            complement(3202..4131)
                     /locus_tag="VFH91_09540"
     CDS             complement(3202..4131)
                     /locus_tag="VFH91_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863281.1"
                     /translation="MPVTDTTATSETPSAHSAPVPKRITNAQTVCNEKNEKGKLCNGH
                     LKQLRTAGEPAEIHLRGDDVLSKCQTCGTLYMGPPLGHVRDPYKQTRFVEAELTALLQ
                     AAGGTLPVIKKDERGVFVMVESAPADHGAAAKPAPSKTQAAAPKAAAPKPAVAPATPK
                     PAEHKAGAQPNRALFKRSTYAGVTDTGPVPGETFEQKVARLKALSAGAKQRAEEGGEP
                     TSAAAAVPTSASAAVDAESAPIAAPAATQPAPNAPAASEHKATGNADRTLMKRSTYCG
                     VVDTGPVPGETFQQKVARLAALSAAAKQRSEGG"
BASE COUNT          984 a         1038 c         1102 g         1091 t
ORIGIN      
        1 cctgacatct cgtgctcgag ttcctagttg aacttcgtaa ttttgacagc gctcaggtgg
       61 gtagaactcg tcatgagctc cggcgagatg aaataccgcg aagtttgctg actggtagag
      121 gtcactgcta tcccagtcac caggtatgcc accacatatt ccgatcacgc cgcgcagctg
      181 ctcgggatga gtgaaagcaa aacgataatt cagtgcgcat gactgtgaaa acccaagcaa
      241 gaaaatccgt gttgaatcga cttgaccgcc ggcaaccaaa gctgtgatga ggtccaggag
      301 agcacggtga tgcactgcaa ccgattcttc actcctaaag ttcgtcagcc atccaaaacc
      361 aaacctcaat ggtccgcccg gttcttttgg gtcgcggatg tgttgatgaa agccctggag
      421 tgaagctatg gcaaaattct gaggcgccat ctgttgagct tccctcatca tctgtcgctt
      481 gtttgcgccg taaccatgta gagcgatcag taaaggtgat ttcgattgcg ctgctggttt
      541 gaagtcgtaa taaagattga cctcggcttt gagaggacgg tcgaggtttc gatcgggggt
      601 gtccatagag tctttatcgg tctatcactg gaaagatgtc aatgctattc ggactccgcg
      661 actcttcggt gaactgcttt ttttaccgcc gagtcgctga gacgctgagg tagcgagagg
      721 ttgattggaa atcctcgatt gcatcaacct caaatgacgg ctacgattgg ttttcaattt
      781 agatcgcgcg accacaagtt tagagtgagg tgaaaagatg aaattccctg gtggattcaa
      841 tattcaacaa atgatgaagc aggcgcaggt aatgcaggaa aagatggcga aggagatgga
      901 agagctgcgt gttgatgctt catccggagg cggtgtcgtc acggtccaga tgaagggaaa
      961 ccatgaaatt ctggcactca aaatcgatcc agaggcattg aaagatggag acgccgaaat
     1021 gcttcaggat ctgattgtcg ccgccgtgaa cgaagccaat cggaaaatcg acgaagccat
     1081 gaaaggcaaa ctcggtggca tgctcccgcc cggcctcggc ggattatttg gttaaagctt
     1141 ttggcctttg gccttcggtc tttgggcttt gatttctgtc aggcccgaag accaaacgca
     1201 ccaaagacca aaaaccaaag atcaaaggcc aattcactta aatgcttgaa tacgctgaac
     1261 cggtaactaa actaattgat gagttaaagc gcttgcccag tatcgggcat aagtctgcgc
     1321 agcgccttgc ttttcatatc atgcgcacgc ctgagtcgga tctcgagcgc ctgattgccg
     1381 cgctacgcga cgtaaaacaa cgaatcgttc attgctcggt ctgcaacaac ctcactgaag
     1441 tcaatccatg ccgtatttgt tcgtctccct cgcgcgatca ttcggtgatc tgtattgtcg
     1501 aagagccgta tagtctggtt gcggtcgaaa agacaaggag ttttaaaggg gtctatcacg
     1561 tgcttcacgg cagtctctca cccatcaggc atattggacc agacgagctc caactcgcca
     1621 atctcttgcc ccgttttaaa cctgaaaaca acgatggtgt cgctgttcgt gaggtgatcc
     1681 tggcgactaa tcccactact gaaggcgagg cgaccgcaaa ctacatcggt cgccttttga
     1741 aacctctggg actaagagtc actcgaattg cgatgggaat gcctgttggc agcgaccttg
     1801 agtacgtgga cgaagtgact atggataaag cactcgccaa ccgtcacgag atttaatgtc
     1861 taaactagaa ccgtacctgt tctgaagtca cgttgtctct gcactctttg cagggttcat
     1921 ggctctcata catcttcacg tcataatcga ccagcgattc aatcggaacg ccttcaactt
     1981 cttttggagc ggaatcgaac cttacgattg caccgcatcc gattatccga gctccaagtg
     2041 actttactaa ggtaactgtc tcttcaatcg cgtttccact gcgaatgata tcgtcgacga
     2101 tgatgcaggg atgcacttcg ccttgattca catactggcg aaacatcctt ttaccttgtt
     2161 cctgttcggc ccagtagatt tgctccgcgt tgagagcgtc gcgaacaccg aacgctaccg
     2221 ggataccacc cgaacttgga ctaatgacgg aaactttagg cagctgactt gaaatatctt
     2281 tctcaagtcg aaattttcgg ctgagagcta cggccaacac gcgagcggtg tcgtagtaac
     2341 gaaaggccag cggcatctga aagtaatgag gcgagtgttt gccgtttgga taaacaaaat
     2401 gaccttcgcg aaacgctcct gtgcgttgca aaatttgcat gattttgtcc ggtgaaggaa
     2461 tgagttcaac catcgcgtta ctcccgttat ctttcgaatc aattcaagtt cgtcatcgaa
     2521 taagtatttg ttggatttgc cgttgcggcg cgtagcatac atcaagtctg cgcttttcct
     2581 ctatcgccca aagcgcgctg gacaatcagt cgttgatcct tcggtaaacc ttcgggtaaa
     2641 gagtcgggcg agaaccaggt cgcgcgatca atttctatac ttttttgttt tgcgtcgccc
     2701 gaggagaagg catgaaaaag aatttctacc tgttgtggtt ttttaaagga ccgtgcagta
     2761 aaaaattcta ctcgttctat ctcgagtcca atttcttcgc gcaactctcg acgcagtcct
     2821 tcgagaggct gttctccgcc ttccaaaaat cccccgggta agccccagcc gctgccggtg
     2881 cgaaatgtgt gtttgagtaa gagtacttta ccttcctcgt tctggatcac tgcgcccgca
     2941 gtcacggtaa atcgcgcgtg agtcatcctc atggtgagac ggcgaagacg agcaggcgtg
     3001 tatcgccaaa gcttgccgag cagtttttgg atcattcaaa gcgaggataa cacgtggcaa
     3061 agtcagaaac cgtgacgtca gcgcgttacg actgaccatt tcgttgtcag attttgtcag
     3121 atatgaaatt cttgtgagtt ggagaagggg tagagcagcg ccgggcttat ggggcgtgac
     3181 cctcctggat ttcactctgg ctcaaccgcc ttcagatcgt tgcttggcgg ctgcggaaag
     3241 agccgccaac ctcgcaacct tctgttggaa ggtttcacct ggcactggac cagtgtctac
     3301 aactccacaa taagtggaac gcttcatcaa cgttcgatca gcgttacccg tagccttgtg
     3361 ttcggatgcg gctggagcgt tcggagcagg ttgagttgca gcaggagcgg caatgggcgc
     3421 tgattcagca tccaccgccg ctgaggctga ggttggaact gctgctgccg ctgatgttgg
     3481 ctctccacct tcttcggctc gctgttttgc tccggccgac aatgctttta aacgagcgac
     3541 tttttgctcg aacgtctcac caggaacggg acccgtatca gttacgccag cgtaagttga
     3601 tcgtttgaat agtgctctat ttggctgtgc accggctttg tgttcggcag gttttggagt
     3661 agccggggcg acggcaggtt tcggcgccgc tgccttggga gcagctgcct gtgttttaga
     3721 cggcgctggt ttagcggcag ctccgtggtc cgccggagca ctttctacca tcacgaaaac
     3781 gccgcgctcg tcctttttta tgactggtaa tgtaccgccg gcggcttgta acaaagcagt
     3841 taactcagcc tcgacaaacc gggtttgctt gtatggatct ctcacatggc ccaatggtgg
     3901 tcccatgtaa agtgttccac aggtctgaca cttggagagt acatcgtcgc cgcgcaaatg
     3961 aatctcggca ggttcccctg cggtgcgcag ttgtttgaga tggccgttgc acaatttgcc
     4021 cttctcattt ttctcattac agaccgtttg agcgtttgtg attcgcttcg ggacgggtgc
     4081 tgagtgagcg gatggagttt ccgacgttgc tgttgtatcg gttacgggca tttttgtttg
     4141 accggcttaa tcggaagata gattgtgcaa ctcgaatctt acaaatgatg tttgattatc
     4201 gtcaatgaaa taggg
//
LOCUS       DASSFP010000423         4828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2069391, whole genome shotgun sequence.
ACCESSION   DASSFP010000423 DASSFP010000000
VERSION     DASSFP010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069391"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..430)
                     /locus_tag="VFH91_09545"
     CDS             complement(170..430)
                     /locus_tag="VFH91_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863282.1"
                     /translation="MPAYNKRPSTVVLMALWCFDICRPSSGLRPNTNNDGRSFARDPN
                     YGPLDRPECLSVLGFHRTLPRNTRFAAHTLPFKAPRRIIDYF"
     gene            449..2050
                     /gene="cimA"
                     /locus_tag="VFH91_09550"
     CDS             449..2050
                     /gene="cimA"
                     /locus_tag="VFH91_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET6863283.1"
                     /translation="MQNKIEIYDTTLRDGTQGESINFSVADKIRIAEALDNLGIDFIE
                     GGWPGSNPRDVAFFEEARNLRLSHARIAAFGATRRQRLSCDEDPNIQALVAAETPVVT
                     IFGKSWELHVNDALRISPEQNLELIEDSIRYLSARVDMVIYDAEHFFDGYRSNPEYAL
                     QTLRAAASGGAQRIVLCDTNGGSLPDQVSEATLVVSEQINLPIGIHCHNDSELAVANS
                     LAAVNAGATHVQGTINGYGERCGNANLCSVIPNLELKLGKEVVGSGQLRHLREAANFV
                     SEIANLSLQSSAAYVGDSAFAHKGGVHVSAVARNPGTYEHIEPELVGNRRRVLVSDLS
                     GKGNLIAKAQELDIDLDGEEGVLEELKRLEHHGFEFEAAEASFELLVRRLRSSPQPYF
                     ELIGFRVIDEHQGNRMPMSEATVKVRVGDHEEHSVASGNGPVNALDRALRKALTEFYP
                     SLKEVHLIDYKVRVLRSSLSGSASLVRVLITSGDGNRKWGTVGVSANIVEASWRALVD
                     SVEYKLMKDASDLMTRAVEEATVIL"
     gene            complement(2224..2745)
                     /gene="msrA"
                     /locus_tag="VFH91_09555"
     CDS             complement(2224..2745)
                     /gene="msrA"
                     /locus_tag="VFH91_09555"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6863284.1"
                     /translation="MNSSTERAVLAGGCFWGVQDLIRRKDGVISTRVGYSGGDVPNAT
                     YRNHGTHAEAIEIIFEPQKISYRELLEFFFQIHDPTTVNRQGNDRGTSYRSAIFYTND
                     EQKRVADETIEDVNASGLWPGKVVTEVTPASDFWEAEPEHQDYLERYPNGYTCHYVRP
                     DWKLPTKVVSMNR"
     gene            2943..3887
                     /locus_tag="VFH91_09560"
     CDS             2943..3887
                     /locus_tag="VFH91_09560"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET6863285.1"
                     /translation="MSFDQSKWVWRNGEIIPWSEATTHVSTHALHYGSAVFEGMRCYE
                     TDDGPAVFRMNEHLERLNRSAAVHGMQIPYDSQELADSICELIDLHDFRSCYIRPICY
                     YGSGSLSLHPGKCPVEVSILVWPWAPYLGAEGLKNGVRITVSPWKKFHSEMMPTTAKA
                     SGQYINSILAVREAVSRGYDEALLLNMDGSVAEGSGENLFVVRKGELFTNDERHSILL
                     GVTRDAVIRLAEDLGFHINVGPISVEMLMTADEAFFTGTAAEVTPIREVDGTVIKDGK
                     RGPVTDLIQKVFFEATSGRDDRYRNWLTYVSRKLVGVD"
     gene            3983..>4828
                     /locus_tag="VFH91_09565"
     CDS             3983..>4828
                     /locus_tag="VFH91_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6863286.1"
                     /translation="MKVFAVAGLLILTLFLRLHFPLQSPTPPAQPETGPGGKVETNKS
                     VIKNRYGTGAKEYWIYEPDSPKPATAPVVVFLHGWGGMNPFYYGAWIDHLVKRGNIVI
                     YPRYQGGLLTPREEFIPNSLAAIKEALSELQTEHGHVRPDLTRVASVGHSLGGILAAS
                     VAALASESGLPKFRAVMSVEPGITRRPVSVPLADLSQIPADTLLLAVAGDRDTLVEDE
                     DAKRIYYESSRIPAANKNFVKLVTDDHGKPALIASHRAPTALNKDYDNGEAERMDQSA
                     LANRRP"
BASE COUNT         1196 a         1128 c         1299 g         1205 t
ORIGIN      
        1 tctgcggttg gttagggtaa ttttttctta ctgggattgc ttgcgcgtgg ggcctgatat
       61 ggggcgctca gtgacggtcc gcactatttc gcaacgccgg cgctcgaatt ggtgcatagg
      121 ctcgaaaggc ctcgtcgttc gggcacattt agtagatatt tatcgagtcc taaaaataat
      181 caattatgcg gcgaggcgct ttgaagggta gagtatgtgc agcgaaacgt gtatttcgcg
      241 gtagagtgcg atgaaagccc agtacgctca aacattcagg tcggtcaagt ggtccatagt
      301 tgggatctcg agcgaaacta cgcccatcat tattggtatt agggcgcagt ccacttgatg
      361 gcctacaaat atcgaagcac caaagggcca tcaacaccac agttgatggc cttttgttat
      421 aagcaggcat ttcgaaaccg aacttccaat gcaaaataaa atcgaaatct acgacacaac
      481 attgcgcgac ggcactcagg gcgagtccat caacttttct gttgccgaca aaatcagaat
      541 tgctgaagcg ctcgacaatc tcggtatcga cttcattgaa ggaggttggc ccggatcgaa
      601 cccaagagat gtcgccttct tcgaggaagc gcggaacctg aggctgagtc acgcccgcat
      661 cgctgctttt ggcgccactc gccgtcaacg actttcctgc gacgaggatc cgaacattca
      721 agcgctcgta gctgctgaaa caccggtggt aacaatcttc ggtaagtcgt gggaattaca
      781 cgttaacgat gcgttgagga tttcaccgga gcagaatctc gaacttatcg aagattccat
      841 tcgatatctt tcggcacgcg tcgatatggt tatttacgat gcagaacatt tcttcgatgg
      901 ctatcgctcg aatccagaat atgcgctgca aacgctgcgc gccgcagcct ccggtggagc
      961 gcagcgaatt gttctctgtg ataccaatgg aggaagtttg cccgatcaag tcagcgaagc
     1021 gacgcttgtt gtgtctgaac agatcaatct gccgatcggt atccattgtc acaacgatag
     1081 tgaattagct gtggcgaact cgctcgcagc ggtgaatgct ggtgcaacac acgttcaggg
     1141 aaccatcaat ggatatggtg agcgctgtgg taatgctaac ctttgttctg tcattcctaa
     1201 cctggagctc aagcttggca aggaggtagt tggttcgggg cagttgagac acttacgtga
     1261 agcagcaaac tttgtaagtg agattgctaa tttgtctttg caatcgagtg ctgcatacgt
     1321 tggcgacagt gcttttgctc ataaaggagg tgttcacgtt tctgcggtag cgcggaatcc
     1381 agggacgtac gaacacatcg agcctgaact ggttggaaat cgccgaagag tattagtgtc
     1441 agacttgtct ggaaagggaa acctcatagc aaaagctcag gaacttgaca tcgatcttga
     1501 tggagaagag ggtgttctcg aagaactaaa gcgtctggag caccatggct ttgagtttga
     1561 ggctgcggag gcgagttttg aattgttggt gaggagattg cgtagctcac cacaacctta
     1621 tttcgaattg attggcttcc gagtcattga cgaacatcag ggaaacagaa tgccaatgag
     1681 tgaggcaacg gttaaggttc gggttggaga tcatgaagaa cactctgtcg cgagcggtaa
     1741 tggtccagtg aacgcgttgg accgtgcttt acggaaagct ttaaccgaat tttatccatc
     1801 gctaaaagaa gttcatctga ttgactacaa ggttcgggtc ttgagaagtt cgctatccgg
     1861 ctcagcgagt ttggtgcgcg ttctgatcac atcgggagat ggaaatagga aatggggcac
     1921 tgtcggtgtg tcagccaata tcgttgaagc tagttggcgc gcactggttg actcagttga
     1981 gtacaaactg atgaaggatg cgtccgatct aatgacccgt gcagtcgagg aagctactgt
     2041 gatcctgtag ctgagtagtt cttctctgtg cgatctcggt gttctctgcg tctctgtggt
     2101 tagaaaggac ggccaaagat tcaccaccga ggcacagaga acacagagat cgcacagaga
     2161 aaattcctga aattcgttga gttactaaaa agacgccgag aacgcagggg aaacccagag
     2221 aattcagcga ttcatactca caacctttgt cggcagcttc caatcgggac gcacgtagtg
     2281 acaagtgtag ccattcgggt atcgctcaag ataatcctga tgctcgggtt cagcttccca
     2341 gaagtcactc gccggagtta cttcagtcac cactttgccc ggccaaagac ctgaggcatt
     2401 cacatcctca atcgtctcgt ccgcaacccg cttttgctcg tcattggtat aaaagatggc
     2461 tgatcgatag cttgtgccgc gatcgttacc ctggcgatta actgtcgttg gatcgtggat
     2521 ctggaagaag aactccaaca gctcacgata actaatcttt tgtggctcga agatgatttc
     2581 gattgcttca gcgtgtgtgc catgattgcg ataggttgca ttcggaacat cgcctccgct
     2641 atagccgact cgtgtggaaa taactccatc tttgcgtcga atcaagtcct gtacacccca
     2701 aaaacagcca ccagcgagca ccgcacgttc agttgatgaa ttcatatgta attttcctct
     2761 ggtcgttagt atagcgcact tgagaagtgc gttgaattta tggccactta aaaataatct
     2821 attagtcaca cctcgacccg cggcgctaga gttccaggta gagagcagtg ctaaagtgcc
     2881 aggtctctcg tcctcatcct tgtcccgaag agtctcaaaa taaagccgga tggagttaaa
     2941 agatgtcttt cgatcaaagt aagtgggttt ggcgcaatgg cgaaattatc ccctggagcg
     3001 aggccacgac acatgtgtca acgcatgcgc ttcattatgg tagcgccgtg tttgaaggga
     3061 tgaggtgtta tgagactgac gatggaccag ccgtctttcg catgaacgaa catcttgagc
     3121 ggctcaatcg ctcggccgcc gtacatggta tgcagatccc ttacgactca caggagcttg
     3181 cagatagcat ttgtgaattg atcgaccttc acgattttcg tagttgctat atcagaccaa
     3241 tttgttacta cggcagtggc tctctcagtt tgcatccggg aaagtgtcct gtcgaagttt
     3301 cgatccttgt ttggccgtgg gccccgtatc ttggcgccga gggtttgaag aatggcgtgc
     3361 gaatcacggt gtcgccatgg aagaaattcc attcagagat gatgccgacc acggcaaaag
     3421 cgtcaggaca atacatcaat tcaatactgg cggtacgcga ggctgtcagt cgcggttatg
     3481 acgaagcgtt gttacttaat atggatggca gtgttgccga aggatctggt gaaaatctgt
     3541 tcgtagtcag gaaaggtgag ttgttcacga atgatgagcg tcattcgatc ttgttaggtg
     3601 ttacacgcga cgctgttatc cggctagccg aagacctggg attccatatc aatgttggtc
     3661 caatttctgt cgagatgctg atgaccgctg atgaagcctt ctttaccggt actgccgccg
     3721 aagtaacgcc aattcgagaa gtggatggca ccgtgatcaa ggatggtaag cgtggtccag
     3781 tgacggactt aattcagaag gtgttcttcg aagcaacttc cggtcgcgat gatcgatatc
     3841 gaaactggtt gacctatgtt agtcgaaagt tagttggggt tgactgatcc tttgtactgg
     3901 caaaattgca gccaaatgac ctttttgcgc gtctaagact ctggtatttt tgggtatata
     3961 aaacccgctc agagagggct gaatgaaggt ttttgctgtt gccggtctac taatccttac
     4021 gctttttctg cggctacatt tcccgctaca gagtccaacg ccgcctgccc agccggaaac
     4081 cggacccggt ggtaaagtcg aaacaaataa gtccgttata aaaaatcggt acggaaccgg
     4141 ggcgaaggag tattggattt acgaaccgga ttcgccgaaa cctgcgactg cgccggtcgt
     4201 agtctttctt cacgggtggg gcggcatgaa tcctttctac tatggcgcgt ggatcgacca
     4261 tctggtcaag cgcggtaaca ttgtcatata tccgcgatat caaggaggcc tcttaactcc
     4321 gcgagaagag ttcattccaa attctctagc tgccatcaaa gaagcattgt cggaattaca
     4381 gactgagcac ggtcacgtgc gccctgattt aaccagggtg gcgtccgttg gtcattcttt
     4441 aggcggaatt ctggcggcca gcgtggcggc gctggcaagt gagtcgggac tgccgaagtt
     4501 tagagcagta atgtcagttg aacctggaat cacgcggagg ccagtcagtg ttcctctggc
     4561 agatctgtcg caaattcctg ccgacacgct cctgcttgct gttgcaggag atcgcgacac
     4621 attggttgaa gacgaagacg ccaagcggat ctattacgag tccagccgta ttccggcagc
     4681 gaacaaaaac ttcgtcaaac tggtcaccga cgatcacgga aagccggccc tcattgcaag
     4741 tcatcgagcg cccaccgccc taaacaaaga ctacgacaat ggtgaagccg agcggatgga
     4801 ccaatcggcg ctggccaacc ggcgaccc
//
LOCUS       DASSFP010000424         4704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_868738, whole genome shotgun sequence.
ACCESSION   DASSFP010000424 DASSFP010000000
VERSION     DASSFP010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868738"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFH91_09570"
     CDS             <1..845
                     /locus_tag="VFH91_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET6863287.1"
                     /translation="FTIRGYNVRSISPVAPLDTFISSQNVVAASNATGTPLVIPGLPA
                     SIASLGTFTGATGSNVAQLQRSFTSVGGDTQLLGNFEYRIPIISDMVSLAAFADIGSA
                     FNIRSGKDQQFSSNLLPDQPFLSTVGAVACPRFGPGASAPISLSSLAICKDPRLASSS
                     FFSLVARDNRVVSTSELEAAGTGDIDPLTLLPRGFQPVFLRGQAQTTSVVRLSQSLFA
                     NLGDYRSSLGMEVRVQVPVINVPFRLIFAYNPNAREDQIIDGFPFFFNEKKKVIRFSV
                     GRTF"
     gene            927..1541
                     /locus_tag="VFH91_09575"
     CDS             927..1541
                     /locus_tag="VFH91_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6863288.1"
                     /translation="MKIDRLIAAIVLLATAAVFSASAQQQKPAGAAAPAPQTATGPVP
                     DSKMAVIYSDQFLDPKGGIARFSTLLTTLNREFQPRQTELNGLQQRIAAIQKEIADTQ
                     NVADPKTIQQKNDQLAQLQVDYKRKGEDAEAAYGRRRQEIFGPLQVDIGKALEAFAKA
                     RGITVIVDGSQVPMVYADNSIDITKAFIGDFNSKNPATASVPNP"
     gene            1607..2266
                     /locus_tag="VFH91_09580"
     CDS             1607..2266
                     /locus_tag="VFH91_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6863289.1"
                     /translation="MRSHRSIIFSAILVVASAASAIAQRPATPAGQRPATPAPQTPTP
                     SAPANVVLPMSKMAVIYSEAFLDSKTGIAKFNMAITRLNGEFQKAKDELTQMQTRAQQ
                     LQDEVSKLQSAPAGTPIDQRTVQTKAEQLDQLKKDIQRKGEDAQAAYNRRRNEIFAPL
                     QEEVGNALEAFAKARSINIIIDGTQVPLIYAADSIDITKAFIADFNAKNPATATAKPP
                     Q"
     gene            2341..2805
                     /gene="fabZ"
                     /locus_tag="VFH91_09585"
     CDS             2341..2805
                     /gene="fabZ"
                     /locus_tag="VFH91_09585"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET6863290.1"
                     /translation="METLLDALAIQKLLPHRYPFLLVDRIVELVPRERIVGIKQVSIN
                     ESFFQGHFPGAPVMPGVLVVEALAQVGAVLALREIEDRDQKLVLFTGIKEARFRRPVV
                     PGDTLVLEVTALRIGSKVQRMRGEAKVDGQLAADAEIMSIVAPRDAAEKAQG"
     gene            2851..3645
                     /gene="lpxA"
                     /locus_tag="VFH91_09590"
     CDS             2851..3645
                     /gene="lpxA"
                     /locus_tag="VFH91_09590"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET6863291.1"
                     /translation="MSAVSRQTTIHPSAVVGPNAQIGEGCFIGPYSIVGDDVELGDDV
                     RLESHCVIDGHTSIGSETHIFPFVSIGLASQDLKYKGEPSETKIGKRNNIREFVTVHR
                     GTSGGGMLTRVGDDCLIMAQAHIAHDCLIGDGVIMANAATLAGHVIIEDRANVGAYSG
                     IHQFCRVGKEAYIGGYSVVVKDALPFALTVGNHAKCYGLNTVGMKRRGYSREAIDLLH
                     HAFHLLLSAKLNTTQAIERITEEISDSAEVADLLRFIEASTRGVTK"
     gene            3642..4439
                     /gene="lpxI"
                     /locus_tag="VFH91_09595"
     CDS             3642..4439
                     /gene="lpxI"
                     /locus_tag="VFH91_09595"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET6863292.1"
                     /translation="MKFGLIAGNGRFPFMVLEGARQTGASMAVAAIREETDPEIERLA
                     ESVKWVGIGQLGRMIRFFKDEGVEKAIMAGQVKHVQIFSRAVPDARMLKVLLKLPRRN
                     TDSLIGAIASELESEGIELIDSTYFLQDSLPKAGVLTRREPDHHEREDIEYGLEIARE
                     IARLDLGQTIVVRGKACVAIEAMEGTDETIRRGGRLVQKRFTVVKIAKPNQDMRFDVP
                     VVGIPTLNTMVEAGASCLCITAGKTLMFDREEMIRTADKHKIAIVAA"
BASE COUNT         1158 a         1160 c         1220 g         1166 t
ORIGIN      
        1 agttcacgat tcgcggttac aacgtgcgct caatcagtcc agttgcgcca ttggatactt
       61 tcatcagctc gcaaaacgtt gtagccgcct ccaatgcgac cgggacgccc ttggtcattc
      121 ccggattgcc agcttcaatc gcatcactcg gtacatttac cggagctaca ggaagtaatg
      181 tcgctcaatt acagcgttcc tttacgtccg ttggtggcga cactcagcta ttgggtaatt
      241 ttgaataccg aattccgatt atcagcgata tggtgagtct tgcggcgttt gccgacatcg
      301 gctcggcatt taacattaga tcaggtaagg atcaacagtt ctcgagtaat cttctccccg
      361 atcagccgtt tctttctact gtcggtgcag tggcgtgccc tcgttttggc cccggcgcga
      421 gtgctcctat cagcttgagt tcgcttgcca tctgcaagga tccgagactt gcctcgtcga
      481 gctttttcag tttggtggcg cgtgacaatc gcgtcgtcag cacgtctgag cttgaagcag
      541 ccggtacggg cgatatcgat cctttgacac tgcttccccg cggattccag ccagttttcc
      601 ttcgcgggca ggcgcaaaca accagcgttg tccggttgtc gcagagtctg ttcgcaaacc
      661 ttggcgatta ccggtcaagt cttggtatgg aggtcagagt tcaggtaccg gtcatcaatg
      721 taccgtttcg cctgatcttt gcgtataatc cgaatgctcg ggaagaccag attattgacg
      781 gctttccatt cttctttaat gaaaaaaaga aggtcatacg cttcagcgta ggccgtactt
      841 tttaatcatt tgagccaacc atggtcgccg gaaagacaac taatgtcttc cagccacctc
      901 cacgtaagga gttttataat aaagttatga agatcgatcg tttaattgct gctatcgtgc
      961 tgttggcgac cgccgcagtt ttttccgcct cagctcaaca acagaagcct gctggagcag
     1021 ccgctcctgc gccccaaact gccacgggtc cggttcctga cagtaaaatg gcagtaattt
     1081 attcagacca gtttttggat cctaagggcg gtatcgcccg gtttagcaca ctattaacta
     1141 cattaaatcg tgaatttcag ccgcgacaga cggaactaaa tggtttgcag caacgaatcg
     1201 ctgcaattca gaaagagatc gcagatacgc agaacgttgc tgatccgaaa acgattcagc
     1261 agaaaaacga tcagctcgct caactccagg tagattacaa acgcaaaggc gaagatgcgg
     1321 aggccgctta tggtcgacgc cggcaggaga tatttggacc actgcaggtc gacatcggaa
     1381 aagctcttga ggctttcgcc aaagctcgag gtatcacagt catagtggat ggcagccagg
     1441 ttccgatggt atacgctgac aacagcattg atatcaccaa agcttttatc ggcgacttca
     1501 atagcaagaa tccggcaact gcctcagttc cgaatccgta attttcgttt accaacagct
     1561 gggaatttga gcctcgcgtt atgcggggct cgatcagcaa atgatcatga gaagtcatcg
     1621 ctcaataatc tttagcgcaa tcctggttgt tgcaagcgcc gcatccgcta ttgctcagcg
     1681 acccgccact ccggccggtc agcgccctgc gactccagca cctcaaactc caactccgag
     1741 tgctccagcc aatgttgttc ttccgatgag taagatggcg gtcatttatt cggaagcgtt
     1801 tttggatagt aagacgggga tcgccaagtt caacatggcg attacgaggc tgaacggtga
     1861 gttccaaaag gcgaaggacg agcttaccca aatgcaaact cgcgcgcagc agttacagga
     1921 tgaagtcagc aaactgcaaa gcgctccggc gggtactccc atcgaccaac gaacggtgca
     1981 gactaaggca gagcagctcg accaattaaa gaaagatatc cagagaaaag gcgaagatgc
     2041 tcaggcggct tacaaccgcc gaagaaatga gatctttgct ccgcttcagg aagaggtagg
     2101 aaacgctctc gaagcgtttg ctaaggcacg aagtatcaac ataataatcg atggaactca
     2161 ggttccgctg atctatgcag ccgacagtat tgatataaca aaggccttca ttgccgattt
     2221 caacgcaaag aacccggcta cggctactgc taagcccccg cagtaacaaa gaagcttgat
     2281 agcatcccaa acgcggagat gcggcgttgg ttccgtgtca ccgcgttttt tctttggctt
     2341 atggaaacac tccttgacgc actggcgatc cagaaactcc taccacaccg ttacccattt
     2401 cttttggttg atcgcatcgt tgaactggtt ccccgcgaac gaattgtggg aatcaagcaa
     2461 gtcagtatta acgaatcctt tttccagggt cattttccgg gcgccccggt tatgccaggc
     2521 gttctagttg tagaagcact ggcgcaagtt ggagccgtac tcgcactcag agaaattgag
     2581 gatcgagatc agaagttggt tctctttact ggaatcaagg aagcccgttt tcgtcgcccg
     2641 gttgtgccag gcgatactct tgttttggag gtcaccgctt tgcgcatcgg atcgaaggtc
     2701 caacgtatgc gcggtgaagc caaggtggat ggtcaactgg ctgcggatgc tgagatcatg
     2761 agcatcgttg cacctcgcga tgctgccgaa aaagcgcagg gatagattgt cgcatctgct
     2821 tactttgaat ttttaacacc aattggatcg atgtctgccg tttcccgtca aacaactatt
     2881 catccctcgg ctgtggtagg tcctaatgct cagattgggg agggatgctt tatcggtcca
     2941 tactcgatcg taggcgacga tgttgagctt ggtgatgacg tccggctcga gtcgcactgt
     3001 gtaattgacg ggcatacttc aattggctct gaaacacata tatttccctt tgtctcgatt
     3061 ggtctcgcgt cccaggatct gaagtacaaa ggcgagcctt cagagacaaa gatcggcaag
     3121 cgcaacaaca tacgcgaatt tgttaccgtt catcgtggaa catcaggtgg tggtatgttg
     3181 acccgcgttg gtgacgactg cctgatcatg gcccaggctc atatagcgca cgactgtctt
     3241 atcggcgatg gcgtaatcat ggcaaacgcc gcaaccctgg ctggacacgt aatcatcgaa
     3301 gaccgggcca atgttggcgc gtattccggc attcatcaat tttgtcgcgt tgggaaggaa
     3361 gcctacattg ggggatattc ggtagttgtc aaagacgcct tgccatttgc tttaaccgtc
     3421 ggtaatcacg ccaagtgtta cgggctaaat acagtcggaa tgaaacgacg aggttatagt
     3481 cgtgaagcta tagatcttct tcatcatgcg tttcacctct tgctctctgc taaactcaat
     3541 accactcagg ctatcgaacg tatcacagaa gagatcagcg attcagctga agttgcagat
     3601 cttcttcgtt tcatcgaagc ctcgactcgc ggcgttacta aatgaaattc ggactaattg
     3661 caggcaacgg ccggtttcct tttatggtcc ttgaaggggc acggcaaacc ggcgcttcca
     3721 tggccgtcgc tgccattcgc gaggaaaccg atccagaaat agaaagatta gctgagagtg
     3781 tgaagtgggt gggtattggc caacttggtc gcatgattcg attctttaag gacgagggtg
     3841 ttgagaaggc gatcatggca gggcaggtca agcacgtaca aatatttagt cgagcggttc
     3901 ctgatgctcg catgctgaag gttttgctca aactgccgcg tcgcaatacc gactcattga
     3961 tcggagcgat agcttcggag cttgagagcg agggtattga actgatcgac tcaacctatt
     4021 ttctccagga ttcactgccg aaagcaggag tgttgaccag gcgggaacct gatcatcacg
     4081 aacgtgaaga tattgagtac ggactggaga tagcacgtga aatcgctcgc cttgatctgg
     4141 gccagacgat tgtggtgcgc ggcaaagctt gtgtagcaat tgaggcgatg gaagggaccg
     4201 atgaaactat ccgccggggc ggcaggttgg tccaaaaacg gtttacggtc gtgaaaattg
     4261 ccaaacctaa tcaggacatg cgctttgatg taccagtggt aggcatcccg actttgaata
     4321 cgatggtgga agcaggtgca agttgtttgt gcattacggc aggcaagacc ttgatgttcg
     4381 atcgagaaga gatgatcagg accgctgaca agcacaagat cgctatcgta gccgcttagg
     4441 ttttctcttt ctgtgaaact tcgcggacac tgagtcagac caacaacgtc atgaacggcc
     4501 ctttgctggg ctcgtagtag aagacaggct gaaatcaact gaagtgacag tgtgaaaact
     4561 caaccttctc attctcccca agcttcagct tggggttagc gcgttccaat cccaatctcg
     4621 gcaaccgttt taacggtttg cgaaattgcg tcaggcatag gatttttgtt ggttcaagat
     4681 aaaccgttga aacggttgac tgcc
//
LOCUS       DASSFP010000425         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1158681, whole genome shotgun sequence.
ACCESSION   DASSFP010000425 DASSFP010000000
VERSION     DASSFP010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158681"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /gene="secD"
                     /locus_tag="VFH91_09600"
     CDS             complement(<1..1379)
                     /gene="secD"
                     /locus_tag="VFH91_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET6863293.1"
                     /translation="MKKKNLLQRTIIILAVTLIGLYIVIGPRHRPTVNDFKWSGIKSS
                     LRNNIHLGLDLQGGSHLVMRVKTEEFLKRLTEENASAAQNAAKDAGFDVKGGHAETGP
                     GNYRVVLDIGDASKANEVKDAVQKKVELADTTVWSYSNSGTQLSWSMTPAAERTLADN
                     ATTQALNIIDSRINALGIAEPTLQTHGSQSSHQILLQMPGVQDPERVKEILKGESRLE
                     LVHVVSPPSPAPSQTYNTEAEALASLNSSGTVPSNRRVLPYSERMEATNGATTNPEQK
                     PKRWVVVEMPPIIDGSELRNANAIPTRAGGAEDFEINFSLKKSGADKFGAWTGSHINE
                     YMGVVLNGEVKSIAYIKSQIFDSGQITGRFTKQSAEDLALTLRSGALPAPIEYLEERT
                     VGPSLGQDSINSGLRASIVGLGLVVFFMLFYYRGSGVNAVVALLLNMILMLAGLIIFG
                     ATLTLPGIAG"
     gene            complement(1392..1703)
                     /gene="yajC"
                     /locus_tag="VFH91_09605"
     CDS             complement(1392..1703)
                     /gene="yajC"
                     /locus_tag="VFH91_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET6863294.1"
                     /translation="MNTFLLFFQGGLGALGQFLPVILIIGVFYVLLIRPQQKRQRELQ
                     ETIASLKVGDRVVTSGGIKGRITAVRDSSFVIISAEKSTLEVAKGSIAGLDNEQEKPD
                     K"
     gene            complement(1803..3002)
                     /gene="tgt"
                     /locus_tag="VFH91_09610"
     CDS             complement(1803..3002)
                     /gene="tgt"
                     /locus_tag="VFH91_09610"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET6863295.1"
                     /translation="MSSATANTENSPLAFTVDATSGEARAGTLKTRRGMIETPIFMPV
                     GTAGTVKGMRFEDLESPDINAPIILGNTYHLWLRPGIEIIREAGGLHRFIGWDRALLT
                     DSGGFQVWSLGDLRKVSEEGTQFRSHVDGSAKFLSPEVSMEVQAALGSEIVMIFDECA
                     PGRASREETKRSIDLTARWAKRSRVAFDRLQIERKDSGYFRSEGLSGNQALFGIVQGA
                     GHLDLRRESLNQMLEIGFDGYALGGLSVGEEKSVMFSVIEEIAPLLPGNSPRYLMGVG
                     TPEDLVESVARGVDMFDCVLPTRNGRTGQAFTSFGKINIKNASWAKDFRPLDESCKCS
                     VCQRHTRAYLRHLYLTAEMLASILLTHHNLAFFLDTMRGVRQSIRSGDFPKFRREFTE
                     RLHADGE"
     gene            complement(3006..4397)
                     /gene="der"
                     /locus_tag="VFH91_09615"
     CDS             complement(3006..4397)
                     /gene="der"
                     /locus_tag="VFH91_09615"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET6863296.1"
                     /translation="MNSPEPTQAPRPRRLVAIVGRPNVGKSTLFNRLIGERRAIVGDE
                     PGITRDRIYGEVDWAGHRFSLVDTGGIVPDDDAVIPANIFKQAGRAIEESQLLIWVVD
                     ARRGITQLDQELAQLLRSSGKHVIVAANKMDSAKLESESTEFYQFGFDEVFPVSAEQG
                     IGVGELLDGVIDLLDQTRIDPNESETEQPREIRLAIVGRPNVGKSSLLNRILGEDRVI
                     VSPVAGTTRDAIDTVLQSDAQTFRIIDTAGIRRKGKTDEMAEKLSVIMARKSLERADV
                     AIVIIDAEDGATNLDATIAGYAVDSGCSVIIAVNKWDAVADKETNTASEFESTLRTRM
                     KFLEWAPVITISALTGQRVERILPLVLKANLARNFRVSTSQLNDFFEKAIAQPRGSVA
                     PAPAKGGVSRLRVQYLTQAGVRPPTFVVFTAGGKPGLHFSYERYLQNQLRKEFEFFAT
                     PLRIVEKHKKRRK"
BASE COUNT         1103 a         1258 c         1131 g         1218 t
ORIGIN      
        1 ccggcgatac caggaagcgt aagggtcgcg ccaaatatga tgagacccgc gagcatcaga
       61 atcatattga gcaacagcgc cacgacagcg ttaacgcccg agccgcggta atagaacagc
      121 atgaagaaca caacaagtcc aagacccacg atcgaagctc ttaagcccga atttattgag
      181 tcttggccaa ggcttggtcc aactgttctt tcttccagat attcgattgg ggcggggaga
      241 gcgccagatc gcagcgtcag cgcaagatct tccgccgatt gctttgtgaa tcgaccagtg
      301 atttggcccg agtcgaatat ctgactcttg atgtaagcaa tagactttac ctcgccgttc
      361 agaacaactc ccatgtactc gttgatgtgg gagcctgtcc aggcaccgaa cttatcggcg
      421 ccagatttct tcaatgaaaa attgatttca aagtcttctg caccaccagc gcgcgttggt
      481 attgcgttcg cgtttcgcag ttcacttcca tcaatgatcg gaggcatttc cacgactacc
      541 caacgcttcg gcttttgttc cgggttggtt gtagcgccat ttgtggcttc catgcgctcg
      601 ctatatggaa gtacacggcg atttgatgga acagtgccgc tgctattgag cgacgcgagt
      661 gcctcagctt ccgtattgta agtttggctt ggagctggac ttggcggact aaccacgtgc
      721 accaactcta atctcgactc acctttcaag atctctttga cacgctctgg gtcttgaaca
      781 ccaggcatct gcaaaagaat ttgatgtgaa ctctgcgagc catgagtctg cagcgtaggc
      841 tcggcaatac cgagagcgtt gatacggcta tcgataatgt tgagtgcctg agtagttgca
      901 ttgtccgcga gtgttctctc cgcagccggc gtcatcgacc acgacaattg agtgccagaa
      961 tttgaatacg accagacagt cgtgtccgca agttcaactt tcttttgaac tgcgtctttg
     1021 acttcatttg ctttggaggc gtcgccgata tcaaggacga cacggtaatt accaggtccc
     1081 gtttccgcgt gtcctccttt gacgtcaaaa ccggcatcct tggcagcatt ttgggcagcg
     1141 gaagcatttt cttccgtcag acgctttaag aactcttcag tcttgactct catcacaaga
     1201 tgcgagccac cctgaagatc gagtccgaga tgaatattgt tccgaagtga cgatttgatg
     1261 ccggaccatt tgaagtcatt caccgtcgga cggtgacgcg gaccaatgac tatataaagg
     1321 ccgatgaggg ttacggctaa gatgatgata gtgcgttgga gaagattctt tttcttcatt
     1381 actaactctg cttacttgtc aggcttttct tgctcgttat cgagaccggc aattgaacct
     1441 ttcgcaacct ccaaagtcga tttttcggcg ctgatgatta caaagcttga atctcgaacg
     1501 gcggtgattc ttcctttaat gccgccactc gtaacaaccc ggtcgccgac tttcaagctg
     1561 gcgattgtct cttgaagttc tcgttgccgt ttctgctggg gccttattaa tagaacatag
     1621 aacactccga taattaaaat caccggaagg aactggccga gtgctcccag gccgccctga
     1681 aagaaaagta aaaaagtatt cattagtttt ggctttttcg aaggattacg acttcgacat
     1741 ccactctgac gtgaagtgat tacgacctat taaacaaaca taggcgaccc gctcgcgaat
     1801 cgctattcac cgtccgcgtg cagccgctcg gtgaattcac gtcgaaattt agggaagtcg
     1861 ccagaccgga tagattgcct cacacctcgc atggtgtcaa ggaagaacgc cagattgtga
     1921 tgcgtaagca gaatgctcgc aagcatttcg gcagtcagat acagatggcg cagatacgcg
     1981 cgagtgtgcc gctgacagac tgaacattta cacgactcgt ccagcggccg aaaatctttt
     2041 gcccaactcg cgtttttgat gttgatcttc ccaaaagatg tgaatgcctg cccagtccga
     2101 ccattgcgag tgggcaaaac acagtcgaac atatccacac ctctcgctac cgattcgacc
     2161 aggtcttccg gagttcctac tcccatcaga tagcgcgggc tgttgcctgg taaaagcggc
     2221 gcaatttctt cgattacgct gaacatcaca gacttttctt cacctacgct aagtccgcca
     2281 agcgcataac cgtcgaatcc aatctcaagc atttgattca aactctcgcg tcgcaggtcc
     2341 agatgacccg caccttgcac aattccgaat aacgcctgat taccgctcaa cccttcgctc
     2401 ctgaagtatc cgctgtcttt cctttcgatt tgtagacgat caaatgcaac tcgggatctt
     2461 ttcgcccagc gtgcggtcag gtcgatactt cttttggtct cttcacggga agcccggcct
     2521 ggtgcgcact catcgaaaat catcacgatc tccgatccca gcgcagcctg aacctccatc
     2581 gagacttctg gcgacaaaaa cttggcactg ccatcgacat gcgaacgaaa ctgagttccc
     2641 tcttcgctga ctttacgcag atcgccaaga ctccagactt gaaagccgcc ggaatctgtc
     2701 agcaacgctc gatcccaccc gataaatcta tgcaggccac cggcctctct gatgatttcg
     2761 atgccgggcc gcagccaaag gtgatatgtg ttgccgagaa ttatcggcgc attgatatcg
     2821 ggtgactcga ggtcttcaaa tcgcatcccc ttaacggtcc ctgctgtccc aaccggcatg
     2881 aagattggag tttcgatcat gccacgtcga gtttttagcg ttccggcgcg cgcttcgccg
     2941 ctggtcgcat cgacagtgaa cgctaacgga gaattctcag tgttggcagt tgcggagctc
     3001 aaacgctact tccttctctt cttatgcttt tcgacgattc tcaaaggagt tgcaaagaac
     3061 tcaaactctt tacgcaactg attttggaga tatctttcgt aggagaaatg cagaccgggc
     3121 ttgcctccgg cagtaaaaac aacaaacgtc ggcggcctta ctccagcctg agtcaagtat
     3181 tgcactcgta aacgcgagac gccgcctttt gccggcgccg gcgcgacact tcctcgcggt
     3241 tgagcaatag ccttttcaaa gaaatcgtta agctgtgacg ttgaaacccg gaaattcctt
     3301 gccaaatttg ccttcaatac aaggggcaga attctctcga cgcgctgtcc agtcagtgcg
     3361 gagatcgtga tgactggcgc ccattcaagg aacttcatcc tggttcgtaa ggttgattcg
     3421 aattccgacg ctgtatttgt ctctttatcc gcgactgcat cccatttgtt gacagcgatg
     3481 attacagagc acccggaatc gacggcataa cctgcaattg ttgcatccag gttggtggcg
     3541 ccatcttcgg cgtcaataat cacgattgct acatctgctc gctccaaact ctttcgcgcc
     3601 ataatgaccg acagcttttc agccatctcg tcagtctttc ctttgcgtcg tatacctgcg
     3661 gtgtcgatta tgcgaaaggt ttgagcatca ctctgcaaaa ccgtatcaat tgcatctcga
     3721 gttgttccgg cgacaggcga tacgatcact cggtcctcac caagtattcg attgagcaac
     3781 gatgatttgc caacgttggg cctgccaacg atcgccaaac ggatttcgcg cggctgttct
     3841 gtttcgctct catttgggtc gatacgagtt tggtcgagga ggtcgatcac gccatcaagc
     3901 agttcaccga cgccaatccc ctgctctgcc gaaactggaa agacttcgtc aaatccaaat
     3961 tgatagaact ctgtactctc actctctagc tttgcagagt ccatcttgtt tgccgcgaca
     4021 atgacatgtt tgcctgatga gcgcaggagt tgagcgagtt cctggtccaa ctgggtaata
     4081 ccgcgtcggg catccacaac ccaaatgagc agttgcgatt cctcgatagc tcgaccggcc
     4141 tgtttaaaaa tatttgcggg aataactgca tcatcatctg gaacgatgcc accagtgtcg
     4201 acaagggaga acctatgccc ggcccaatcg acttctccgt agatgcgatc cctggtgatt
     4261 cccggttcgt cgccgacgat ggcccgtcgc tctccaatca atcggttaaa gagcgttgat
     4321 ttgccgacat tagggcgtcc gactatggcg acgagtcgtc gcggtctggg tgcttgcgtt
     4381 ggttctggtg aattcattgg attcctgaaa aagaatagaa ggtaggacaa aagttggtgg
     4441 aatgccagtg ggtgggctgt ttaaggttca atttccatat ctcatttttc gtatctcatt
     4501 ttccatttct catttttcat ttgattccga cgttgactct gtacgcatta gacgtcggaa
     4561 ttacagaaca gcgagcgaag cgactgtgca ctttcaacga cctacgctct gtatgggctg
     4621 cagcctacct cgttacagaa cagcgagcga agcgactggg cacttaataa ctgacgctgc
     4681 aggttgaatt cagtcgcttc gcttgtggtt
//
LOCUS       DASSFP010000426         3924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_868761, whole genome shotgun sequence.
ACCESSION   DASSFP010000426 DASSFP010000000
VERSION     DASSFP010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868761"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFH91_09620"
     CDS             complement(<1..396)
                     /locus_tag="VFH91_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863297.1"
                     /translation="MKGAIVHPGSPLHLKGVISMTRLSSLILGMISFLTLTVSIVQSQ
                     EQGADSNRLASRSQHPSVTHIQQPSSPVILKISPVKSIPEKPGLVKITFKAQGAKTVK
                     GYHFHYEENFVDKDGARGSVATDSTSLRTL"
     gene            complement(508..864)
                     /locus_tag="VFH91_09625"
     CDS             complement(508..864)
                     /locus_tag="VFH91_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863298.1"
                     /translation="MTYFNLVSAEGENLQVIDWLWNPTLQLLFSADAITQEDHLRLLT
                     QGFGAQVDAAKATLIGDIVAQKVGGANPGRMQTDRLVTSESEVLAAFGPNTEITYKWL
                     RMFAKFCQSSGGFEVV"
     gene            complement(1496..1915)
                     /locus_tag="VFH91_09630"
     CDS             complement(1496..1915)
                     /locus_tag="VFH91_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863299.1"
                     /translation="MKQTSLFQNAKRLFEFIALIAVIAMWCCPTIAFACSGETMDLIY
                     HNRLIVQICAALSIVLFVTTVVLYFLRGRKGLWVVVASFMLVVFHPAWINGGGAADCG
                     RSMSSGAKFWVVLLGIGAAHQLRSWLINRRNEIGGRI"
     gene            complement(2031..3782)
                     /locus_tag="VFH91_09635"
     CDS             complement(2031..3782)
                     /locus_tag="VFH91_09635"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET6863300.1"
                     /translation="MRKVFYKPILLSGVFAVTALVPLAQQTSSPTTAVMAQQIEQGKP
                     VERELKSKETHAYQLTLAAGQFLNAAVNQRGIDVVVRVFAPDEKLVVEIDSPNGAQGD
                     EPIALEAKNAGTYRIEVIPNEEGGNKASGRYEIRINEILSPDTYANRLAEHKRAQASV
                     VAWLRENAVPVKTVEAGNSFDDLQPLKRILRNVRFVALGEATHGTREFFQFKHRMVEF
                     LVREMGFRVFAIEASFSACQNINDFVMGKTDDGAKALDSQGFWTWNTEEVSSMLDWMR
                     AYNQGVPAEKRVKFAGIDIQINNTGKAKLLEYLKLVAPERSAEMVASFQVEEGQLINA
                     AFGGQDDKSKEARAKLADLRAQYNGLFIFLELHGAELAAKSSSKEYEQMREYARVLVQ
                     FMDSYSEGSSALRDLYMADNVRRLVEREPINTRVIIWAHNGHIMTDNSDATNLPFGAH
                     LRRFFGNEYYALGFSFNQGTFQAREAQPKDPANRMLMSFTAHPAPADSLDWYLAQTGL
                     KSFIIDFRSARKNANGTEWLTTTHPMRSVGSVYAPSAENSTFSATTLSRDFDGLFFIE
                     TATRARPNQSVKNVVRE"
BASE COUNT          957 a         1045 c          960 g          962 t
ORIGIN      
        1 caatgtcctc agcgaagtcg aatcagtcgc gacagaacct ctggcgccat ccttatctac
       61 gaagttctcc tcataatgaa aatgataacc tttgacggtt ttcgctccct gcgctttgaa
      121 ggtaattttc accagtccgg gtttctcagg gattgacttg acaggagaaa ttttgaggat
      181 tacgggtgaa ctcggttgct gtatgtgggt cactgaagga tgttgactcc tactcgccag
      241 acggttgctg tctgcacctt gctcctgact ctgaacgatc gagactgtca aggtcaagaa
      301 gctgatcatt ccaagaataa gtgacgagag ccgagtcata ctgatcactc ctttcaaatg
      361 taagggagag cctggatgga ctatagcacc tttcatttaa cgaagctgtc taacgaatag
      421 gatgctttcc gaagtcccgc cttccgatta cgagagagca taacgcccga gtccgcccta
      481 agcgcttatg ggttgttcgg cggcgcgcta aactacctca aagcctcccg aactctggca
      541 gaacttcgca aacattcgca accatttata tgtaatctcc gtgtttggcc cgaaggcagc
      601 aagcacttcc gattcgcttg tcaccaatcg gtctgtttgc atccgccctg gattggcacc
      661 cccaactttc tgcgcgacga tatcaccaat caaggttgcc ttcgccgcat ctacttgggc
      721 cccaaacccc tgcgttagca aacgcagatg atcttcttgt gtgattgcat ccgcagaaaa
      781 caataactga agcgtgggat tccacagcca gtcaattact tgaagatttt cgccttctgc
      841 ggaaacaaga ttgaaatatg tcatctacga gaagccgccg aacgcccggc atcgcccgcg
      901 cgcccctacg caacgtacat gagaggcagc gtgacgggac gtcgggtgca tgccgttgtt
      961 aggcgtgggt tacgattaaa caagacatag gatatagcag ttctatttca tgtacaaaaa
     1021 aggtcgagat tgaccaaata gtctatcccg acctcattta ctctgaaaat tgacgcttaa
     1081 cttagagagc ctgttgatgt ctctttctcc aatgtaaggc aaatttgacc aagcctccta
     1141 agaccgacca gatcatgcct acatacagaa tccaattagt gggattttct tcgtgaaaga
     1201 ccaataacca aatgctaatc ccgaataaga cagcgtaaag ggccgagtag acgaccaggt
     1261 tcgggatttt tgtcttcata tcaatcttca tcgaaatatc tccagccacg tttctaaagc
     1321 cgagggtgag cgaagtgttt atcggctcac cagaagatta aacaacgttt ggcagcctag
     1381 tcaagaaaga tttactgggt ttttcaagaa agtagcgcct cacgctgctg cgcttcagcg
     1441 gctcgcccac gcaacttcga ataagaatta ctcaccggcg agtccgctgg aagcgttata
     1501 ttcgcccgcc gatttcattg cgccgattaa tcaaccagga tcttagttgg tgagcggctc
     1561 caattcctaa caacacgacc cagaatttcg cgccactcga catcgagcga ccgcagtccg
     1621 cggcacctcc gccattgatc caagcaggat gaaaaacgac caacatgaaa cttgcaacga
     1681 caacccagag gcctttccta cctctcaaga aataaagaac aactgtagtc acgaacaaga
     1741 ctattgagag tgctgcgcag atctgaacta tcagtcgatt gtggtaaatc aaatccatcg
     1801 tctctccact gcacgcaaaa gcgatcgtcg gacaacacca catcgcgata accgcgatca
     1861 acgcgatgaa ctcgaatagg cgcttggcgt tttggaatag agacgtttgt ttcattttta
     1921 gcgagcgcat aacgctgatg cgcttcagcg actcgccccc gcaactatga atagagacta
     1981 atccatcgcg agttcgcttc gttggctgcg atgaataata gcaatggtct ttactcccgg
     2041 acgacgtttt tgaccgactg gttcgggcga gcgcgtgtcg cggtttcaat aaagaacagt
     2101 ccgtcgaagt ctcggcttag tgtggtcgcc gagaacgtac tgttctctgc actcggtgcg
     2161 tacaccgaac cgactgaacg catcggatgc gtggtggtaa gccattctgt gccattcgca
     2221 ttcttgcgag cagagcgaaa atcaatgatg aaactcttca agcccgtctg agcgagatac
     2281 caatcgagcg agtcagcggg cgcggggtgc gctgtaaaag acatgagcat cctgttcgca
     2341 gggtctttcg gctgcgcctc tcgcgcttga aacgtgccct ggttgaagct gaaaccaagc
     2401 gcgtaatatt cattgccgaa aaagcggcgt aggtgagcgc cgaaaggcag gttggtcgcg
     2461 tcgctgttgt ctgtcatgat gtggccgtta tgcgcccaga tgataacgcg cgtgttgatt
     2521 ggttcgcgct cgacaaggcg acggacattg tccgccatgt agagatcacg tagggcggat
     2581 gacccttcgg agtacgaatc catgaactgc accagaacgc gggcgtattc gcgcatctgt
     2641 tcatactctt tggagcttga tttcgccgcg agttctgcgc catgcagttc taaaaagata
     2701 aacagaccat tgtattgagc acgcaaatca gcgagcttcg cccgcgcctc tttcgacttg
     2761 tcatcttgcc cgccaaacgc ggcgttgatt aattgtccct cttcaacctg gaaagaagca
     2821 accatctccg cactccgctc cggtgcgacg agtttcagat attcgagcaa ctttgctttg
     2881 ccggtgttgt tgatttgtat gtcgatgcct gcgaacttta ctcgcttttc tgcgggaacg
     2941 ccttgattgt acgcccgcat ccaatcgagc atcgagctca cctcttcggt attccaggtc
     3001 caaaagccct gactgtcgag agccttcgcg ccgtcatcag tcttacccat tacaaagtcg
     3061 ttgatgttct gacaggccga aaaactcgcc tcaatcgcaa agacgcgaaa gcccatttcg
     3121 cgtacgagga actccaccat gcggtgtttg aactgaaaga actcgcgtgt gccgtgcgtc
     3181 gcttcgccaa gggcgacgaa tcggacattt cttaagatac gcttgagcgg ttgcagatca
     3241 tcaaaactgt tacctgcttc cacagtctta acgggaaccg cgttctctct gagccacgcg
     3301 acgactgagg cttgcgcacg tttgtgttcg gcgagccggt tggcgtaggt atccggggag
     3361 aggatttcgt tgattctgat ctcgtaccgt ccggaggctt tattgccacc ctcttcgttt
     3421 gggataactt cgatgcgata cgttcctgcg tttttagctt cgagcgcgat gggctcgtcg
     3481 ccttgagcac cgttcggcga atcgatctcc acgaccaact tctcgtccgg cgcaaagaca
     3541 cgcacgacga catcaatgcc tcgctggttg accgccgcat ttaagaactg acccgctgcc
     3601 agcgtaagtt ggtaagcgtg cgtctccttg gacttcaact cgcgctcgac aggcttgcct
     3661 tgctcaatct gttgggccat gaccgctgtg gtcggcgaag atgtttgttg agctaagggt
     3721 actaaagcag taacagcaaa aacgccgctt aacagaattg gtttgtagaa tactttgcgc
     3781 ataattagtt ttcctcacgc tgtgccagga taacgattaa tgcgaagcaa ctgccaaacc
     3841 cgacaccttg tcctataaac gccggagcga atgtgccgct tttgtgccga tttcaatcaa
     3901 tcgcccatta gatatcatag tagc
//
LOCUS       DASSFP010000427         6676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1016370, whole genome shotgun sequence.
ACCESSION   DASSFP010000427 DASSFP010000000
VERSION     DASSFP010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016370"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="VFH91_09640"
     CDS             complement(<1..1547)
                     /locus_tag="VFH91_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6863301.1"
                     /translation="MIRNRPASIPLVLSSVFLLLLFPPANAQTPTPTLDGVWNGVLAI
                     GDAKLRLALKITKTPDGLTAVMDSVDQPNGGNLKVDSITLQDNVLRFEMKTLQIVYEG
                     TLNKDGFEIAGTFTQATQPFPMIFRKEGAPVVTTVTKRGRVDLRPCNNPTLTGDILCG
                     KYEVFEDRAARKGRKINLNIVLLPALSSKPATDPLFYLAGGPGGAATSYAAEKFMSRL
                     RRNRDVVLVDQRGTGQSNPLNCPPAGSREDMRGFFGEVMPVDRIRACRTELEKVADLK
                     LYTSSIAMDDLDEIREVLGYDRIDLYGGSYGSTTALVYLRQHPKHVRAAAVFGVSPPS
                     AKIPLSFAKGVQDSMDRLFADCAKDSTCHEAYPDVEKEFQTVLARFEKGPVEVDVPNI
                     YTGAQQKVTVTRDAFVDSIRHLLYVPAATSALPALIHHAAAGKLGPLIGTAFQIVLQI
                     DNKIARGMQFSVLCAEDTPFITEEEIKQTSGKSFYGDARVRPTIRACGEWPAQKSPRT
                     FLEPVKST"
     gene            complement(1787..6010)
                     /gene="rpoC"
                     /locus_tag="VFH91_09645"
     CDS             complement(1787..6010)
                     /gene="rpoC"
                     /locus_tag="VFH91_09645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET6863302.1"
                     /translation="MFRFQQEKTQLAPDFEAIRISLASPEKIRQWSHGEVTKPETINY
                     RTFKPERDGLFCARIFGPVADWECLCGKYKRMKHRGVICDKCGVEVTQAKVRRERLGH
                     IDLASPCSHVWFFKGLPSRIGHLLDIPLRELEKVLYFESYIVIDPGDVPGLKERELVS
                     DERYRELVRDYPAQFVAKMGAEAIKELLAMVDVEELATDLRKRMREETSQQKKLKYSK
                     RLKVATSFLKSGNRPEWMILDVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNN
                     RLKKLMELRAPEVIVRNEKRMLQEAVDALFDNGRRGRVLRGVNNRPLKSLSGTLKGKQ
                     GRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYNQLEKQGHAAT
                     IKQAREMVERQEPVVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLVEGKAIKIHP
                     LVCTAFNADFDGDQMAVHIPLSPEAQIEAAVLMLSSNNILSPASGQPIAVPSQDIVLG
                     IYYLTRDRPGAKGEGRVFASLEEVLLALDANEVTTQTPIKLRIQGDLIDLVQEHDPQD
                     ILRATVQDDVRRVINTTVGRVIFNDSIPAGLPFINGTLKKKGLQSLVNYSHIRLGHEM
                     TVKMLDDLKTLGFLYATKAGISIGIDDLVTPAAKKPLVEKADKDVIAVEQQYLDGVIT
                     NGERYNKVIAIWSEVTDKVAKEMFKAMDDRERTSGELNPILVMSDSGARGSAQQIRQL
                     AGMRGLMARPSGEIIETPIQANFREGLNVLQYFISTHGARKGLADTALKTADSGYLTR
                     RLVDVAQDVIISEDDCGTLRGISASAIVEGGEEIESLRDRIVGRTALEDVTDPIEGSA
                     IVEANQEIDEDLAAEIQRSGVQRVRIRSVLTCESRRGCCIKCYGRNLATGRPVEIGEA
                     VGVIAAQSIGEPGTQLTMRTFHIGGTARLEESSKHEARQDGTIKYVDLQTVKSRKGER
                     IAMNRNGSLTIIDDRGREAARYQIVYGAHILVEDGSRVTQDQLLATWDPFTFAILTEV
                     AGHVKFQDLKEGVTFDEEVDEVTGQSRMVVKDSPDEKKQPRLEIRSSSNKVLKTYQMP
                     VRANLMVTDGEEVEPGDIIAKIPRETTKTKDITGGLPRVVELFEARRPAETAVMSEID
                     GTVTLGAISKGKRKLIITGDDGEEREYDIPRGTHINVQDGDRVRAGEALMDGPLNPHD
                     ILRVLGMSRLQEYLVNEIQEVYRLQGVNINDKHIEVIVRQMLRWVKIKEVGDTEFLLE
                     EQVDRFRFQDENERVRVEDGEVAQAEPLLLGITKASLSTDSFISAASFQETTRVLTEA
                     AISGRVDYLRGLKENVIMGRLIPAGTGMEYYRNVDVERDETIDQGRERDLEEFPDIVG
                     GHDITLPPREPIAVAASDGDGAVPVKPEE"
     gene            complement(6131..>6676)
                     /locus_tag="VFH91_09650"
     CDS             complement(6131..>6676)
                     /locus_tag="VFH91_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863303.1"
                     /translation="QKLQDSGARARELGLPEIVNESGKAILYDGLSGDPFEQKVTVGY
                     IYMLKLSHLVDDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAHIL
                     QELLTAKSDDVAGRSKIYEAIVKGEADFDPGLPESFNVLVRELQSLCLDVELINKKAA
                     GDGTGLEDGVGEAIATSAVEA"
BASE COUNT         1511 a         1840 c         1670 g         1655 t
ORIGIN      
        1 gttgatttta ccggctcgag gaacgttctg ggagactttt gcgcgggcca ttctccgcag
       61 gcacgaatgg tcggacgaac tctagcgtca ccgtaaaaac tcttgccgct ggtctgcttg
      121 atctcttcct ccgtaataaa cggagtgtct tccgcacaga gcactgagaa ctgcattcca
      181 cgagcaatct tattatcgat ctgcaagacg atttggaaag cagtaccaat caatgggcca
      241 agctttccag ccgccgcatg atggatcaaa gcagggagtg cagaagttgc agccggaacg
      301 tagagaaggt gcctgatcga gtccacaaat gcatcacgtg tcactgtgac cttctgttga
      361 gcgccggtgt agatgttcgg aacatcaact tcaaccggcc ccttctcgaa tcgagcgaga
      421 acggtctgaa attctttttc gacgtccggg taagcttcat gacaggtgga atccttggcg
      481 cagtccgcaa aaagccggtc cattgaatcc tgcacgccct ttgcaaacga cagcgggatc
      541 tttgcggatg gtggggatac tccaaagact gcagctgccc gcacatgttt cggatgttgg
      601 cgcaagtaga ccaaagcagt cgttgatcca tatgagccgc cataaagatc aatgcggtcg
      661 tacccaagca cctctcttat ttcgtcgagg tcatccattg cgatagatga tgtatacagt
      721 tttaagtccg cgaccttttc caattctgta cgacacgcgc ggatacggtc gacaggcata
      781 acttcgccga agaagccacg catgtcttct ctactgccag ctggaggaca attcaggggg
      841 ttcgactgtc ctgtcccgcg ctggtccacc agcacgacat cgcggtttcg cctcaacctg
      901 gacatgaact tttccgcggc ataactagtt gcggcaccac ctggcccacc tgcgagataa
      961 aacagcggat ctgtcgcagg cttcgagctg agcgcaggta agagaacaat attcagattg
     1021 atttttctac cctttcgggc ggcgcgatcc tcaaacacct cgtacttacc acagaggatg
     1081 tcgccggtaa gagttggatt attgcagggt cgtaaatcaa ctcggcctcg tttagtgaca
     1141 gtagtgacta caggcgcgcc ttctttacga aagatcattg gaaagggctg cgtagcctgg
     1201 gtaaaagtac cggcgatctc gaaaccatcc ttgttgagag ttccttcata gactatctgc
     1261 agcgtcttca tctcgaagcg aagaacattg tcttgaagtg ttattgaatc gactttgaga
     1321 ttaccgccgt tcggctggtc gacgctgtcc atcaccgcag ttagaccatc cggggtcttc
     1381 gtaatcttca acgcaagtcg aagcttagcg tctccgatgg cgagaacgcc gttccataca
     1441 ccgtcgagtg taggtgttgg cgtctgggca ttcgctggcg ggaacaggag tagaaggaaa
     1501 acacttgata ggactaaggg tatggaggca gggcgattcc tgatcatcga cgcgtttcac
     1561 tttctggttt gtttcatcca ggaagaaatt atcaagctag ctcgctgttg aaaagactaa
     1621 ttttcgtcct cctggaactc tgcgtgatct ctgcgaagaa taggtcctaa taagtcatat
     1681 aggacctatt tttcaccaga gatctcgcag aggtaggcag agagcacata gaaaaaggcc
     1741 cggtcgggat gaccgggcct tctcatggag cgtataggag tgctctttac tcttccggct
     1801 taacgggaac agctccgtcg ccatcgcttg cagccacggc gattggctct ctcggcggaa
     1861 gagtgatgtc gtgtccacca acgatatccg gaaactcttc cagatctcgt tcgcgaccct
     1921 gatcaatcgt ttcgtctcgc tcgacatcaa cgttgcggta gtactccata ccggttccgg
     1981 ctggaatcaa ccttcccatg atgacgtttt ccttcaaacc acgcaggtaa tccacgcgac
     2041 ctgaaatagc agcttcggtc aggactcgag tcgtttcctg gaacgacgct gctgagatga
     2101 acgaatcggt tgaaagcgac gccttcgtga ttccgagaag caacggttcg gcttgtgcaa
     2161 cctcgccatc ttccactcga acgcgctcat tctcatcctg gaagcggaaa cgatcgactt
     2221 gttcttcaag taagaactcc gtgtcgccca cttctttgat cttcacccaa cgcaacatct
     2281 gacgaacaat cacttcgata tgtttgtcgt taatgttcac gccctgcaga cgatagacct
     2341 cctgaatttc gttcacgagg tattcctgca agcggctcat tccgagaact cgcagaatgt
     2401 cgtgcgggtt cagtggtcca tccatcaagg cctcgccggc tcgaacacgg tcgccatcct
     2461 gcacgttgat gtgtgtacca cgtggaatgt cgtactcgcg ctcttcaccg tcgtcgccag
     2521 tgataatgag cttgcgttta cccttggaaa tcgctccgag agtaacggta ccgtcgattt
     2581 cagacatgac cgcggtttcg gcgggccgtc gcgcttcaaa gagctccacg acgcgcggca
     2641 aaccacccgt gatgtccttg gtcttcgttg tttcgcgtgg aatctttgcg atgatgtcac
     2701 ccggctcaac ttcctcaccg tcggtcacca tcaggttcgc tcgaacaggc atctggtacg
     2761 tcttcagcac cttgttgctc gagctccgaa tttccaaacg aggttgtttc ttctcgtccg
     2821 gcgaatcctt caccaccatg cgagactgac ctgttacttc gtctacttcc tcgtcgaagg
     2881 ttacgccttc cttcaagtcc tggaacttaa cgtgaccagc aacttccgta agaattgcga
     2941 aggtgaacgg gtcccatgta gccaggagtt gatcctgagt tacgcggcta ccgtcttcaa
     3001 caagaatgtg cgctccgtaa acgatctggt agcgagctgc ctcacgacct cgatcgtcga
     3061 tgattgtcaa agaaccgttg cggttcatcg cgatgcgttc gcccttacga ctcttaacag
     3121 tctgcaggtc aacgtacttg atggttccat cctgacgagc ttcgtgtttc gacgactctt
     3181 caagacgagc cgtaccaccg atgtggaacg tacgcatcgt caactgagta cccggttcac
     3241 cgatggattg agcggcaata acgccaaccg cttcaccgat ctcaaccggt cgtccagttg
     3301 caaggttgcg tccgtaacac ttgatacagc agccacggcg tgattcgcag gttaataccg
     3361 aacgaatgcg aacccgctgt acgcctgaac gctgaatttc agcagcgagg tcttcgtcaa
     3421 tctcctgatt ggcttcgaca attgctgaac cttcaatcgg gtcagtaaca tcttccaacg
     3481 cagtgcgacc gacaatacga tctcgcagcg attcaatttc ctcaccacct tcgacgattg
     3541 cgctagcgga aattccccgt agcgtgccgc aatcatcttc actgatgatt acgtcctgag
     3601 caacgtcaac caaacgacga gtcaggtaac ctgagtcggc ggtcttgagc gcggtatcag
     3661 cgagaccctt acgtgcgccg tgcgtcgaaa tgaagtactg caacacgttc aagccttctc
     3721 ggaagttggc ctgaattgga gtctcaatga tttcgcctga cggacgagcc atcagaccac
     3781 gcattccagc gagctggcga atttgctgcg cactaccacg agcaccagag tcgctcatca
     3841 ccaggattgg attaagttca ccacttgtcc gttcgcgatc atccatcgcc ttaaacatct
     3901 ctttggcgac tttgtcagtt acttctgacc agatcgcgat gaccttgttg tatcgctcac
     3961 cattcgtgat gacgccatcg agatactgct gttcgactgc gatcacgtcc ttgtcagcct
     4021 tttcaacgag tggctttttc gcggcaggcg taacgagatc gtcgataccg atcgagattc
     4081 ctgctttagt cgcatacaag aatccgaggg tcttgagatc gtcgagcatc ttcacggtca
     4141 tttcatgacc gagtcggatg tgcgagtaat taaccaacga ctgcaaacct ttcttcttca
     4201 acgttccgtt gatgaaaggc aatcctgccg ggattgagtc gttgaaaatc acgcggccca
     4261 cggtggtgtt gattacgcgt cgaacatcgt cctgaacagt cgcacggaga atatcctgcg
     4321 gatcgtgttc ctggaccagg tcgataagat cgccctgtat gcgaagctta atcggtgtct
     4381 gcgtcgttac ttcgtttgcg tcgagtgcga gcagcacctc ttcgagcgag gcaaacacgc
     4441 ggccttcgcc ctttgctccc ggcctatcgc gagtcaggta ataaatgcca agcacgatgt
     4501 cctgtgatgg aaccgcgatt ggctgtccgc ttgcggggct gagaatgttg ttactggaaa
     4561 gcatcagcac cgcagcttcg atctgtgctt cgggactcag cggaatgtgt acggccatct
     4621 ggtcaccgtc gaagtcagca ttgaatgccg tacaaacgag cgggtgaatt ttgattgcct
     4681 taccttcaac gagcactggc tcgaacgcct gaataccgag ccggtgaagt gttggggcac
     4741 ggtttagtag taccgggtgt tctcgaataa cctcttcgag gatgtcccaa actacaggtt
     4801 cttgccgttc caccatttca cgggcctgct tgattgtcgc tgcgtgtccc tgcttctcaa
     4861 gttggttgta gataaatggc ttgaacaatt ccagggccat tttcttcggc aagccgcact
     4921 ggtgcagttt gagttctgga ccaaccacga tgaccgaacg accagaatag tcgacgcgct
     4981 taccgagcag gttctgacga aaacgtccct gcttgccttt aagcgttcca ctgagagact
     5041 tcagcgggcg attgttgacg ccacgaagca cgcgaccacg tcgaccgtta tcgaacagtg
     5101 catcgaccgc ttcctggagc atgcgctttt cgtttcgcac aatgacttca ggcgcacgca
     5161 actccatcaa cttcttcaag cggttattgc gattgattac gcgccggtaa agatcgttca
     5221 agtcagaagt ggcaaaacga ccaccgtcga gtggcaccaa cggtcgcagc tcgggtggaa
     5281 tgaccggaat aacgtcaaga atcatccact caggcctgtt acctgattta aggaagctgg
     5341 tcgcaacctt cagccgcttg gagtacttca atttcttctg ttgcgaagtc tcttccctca
     5401 tccgcttgcg cagatcggta gccaactcct caacgtcgac catcgcgagc agttccttga
     5461 tggcctctgc gcccatcttc gcgacgaact gagcgggata atcgcgtaca agttcccggt
     5521 aacgctcatc acttacgagt tcgcgttctt tcaaacctgg aacatcgccg ggatcgatga
     5581 cgatgtatga ctcaaagtag agaactttct cgagttcgcg cagtggaata tccagaaggt
     5641 gtccaatgcg actcggcagt cctttaaaaa accagacgtg cgagcagggg ctggcgagat
     5701 cgatgtggcc cagtctttct cgacgcacct tcgcttgagt tacttctacc ccgcatttgt
     5761 cgcagatcac gccgcggtgc ttcatgcgtt tgtatttacc gcagagacac tcccaatcag
     5821 caaccggtcc aaagatccga gcgcagaaca agccgtcccg ctcaggtttg aaagtccggt
     5881 agttgattgt ctcgggcttg gtcacctcac catgcgacca ttgccggatc ttttccggtg
     5941 acgcaagtga gattctgata gcttcaaagt cgggggccaa ttgcgtcttt tcttgttgga
     6001 atcgaaacac tttattttct ccgttgcgtt aagaacaaaa actggtcttg gatgttggtc
     6061 tttgatcctg gctctttaat gattaggagt tcctatcaaa ggccaaagtc caaagaccaa
     6121 aaaccattct ttacgcttca acagcgctgg tcgcgatggc ttctcctaca ccgtcttcca
     6181 gcccggtgcc atctccggcc gctttcttgt tgatcagttc cacgtccaga cagagcgact
     6241 gtagttcgcg gaccagcacg ttaaacgatt caggtaatcc tggatcgaag tcggcttcac
     6301 ctttgacgat ggcctcgtaa atcttcgagc ggccagcaac gtcatcggat ttcgcggtta
     6361 agagttcctg aaggatgtgc gccgcaccat aagcctcgag cgcccaaacc tccatttctc
     6421 caaatcgctg tccgccaaac tgtgctttac caccaagcgg ctgctgtgtg atcaagctgt
     6481 atggtccaat ggatcgagcg tgaatcttat cgtcaacaag gtgcgaaagc ttcaacatgt
     6541 agatgtagcc aaccgtcacc ttctgctcaa aggggtcacc actaagcccg tcgtacagaa
     6601 ttgccttgcc ggactcgtta acgatttcgg gaaggccaag ttctcgagct ctagcacccg
     6661 agtcttgcaa cttctg
//
LOCUS       DASSFP010000428         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1456824, whole genome shotgun sequence.
ACCESSION   DASSFP010000428 DASSFP010000000
VERSION     DASSFP010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456824"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFH91_09655"
     CDS             <1..224
                     /locus_tag="VFH91_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863304.1"
                     /translation="RYYVEGILAVIYGRRVLLFLRDNGLVIISMVATIILIGLLIYYF
                     VNKRKVAAIMAEADDQPLDASPDTERVID"
     gene            221..859
                     /locus_tag="VFH91_09660"
     CDS             221..859
                     /locus_tag="VFH91_09660"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6863305.1"
                     /translation="MKSFIHPVTKALTRLSEYLVTFGAFGLFLVAVLDSTFVPLPSSV
                     DALMLLLSATNPRWMLVYALMATSGSALGCWILYVISRRAGARALRRFSAAKQQRVKD
                     LIDRYDMMAVLVATLLPPPFPFKLFVISAGVFRFSPTRFMIAIIGGRAFRFLLEGYFA
                     VRYGAEAKVILAKYYPWIGVGLAVLILAGFIVRRLLRKSREVVVEQSLETGS"
     gene            complement(839..1708)
                     /locus_tag="VFH91_09665"
     CDS             complement(839..1708)
                     /locus_tag="VFH91_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HET6863306.1"
                     /translation="MSPFPNLVSDSTLVQDTIDLLNDSGGRAPASHIVDAVFKLSHID
                     DELASLLVADLIRNDRRFRIGDNHTIELLEDDWGRRLLKDLEFVVVDVEATGAKTPPN
                     RLIELGAFRIRDGRIVDKFLTLVNPEIPIPRFVASLTGISNDMVKRAPVFADVAPRWL
                     DFVSDSVLIAHNAPFDTSFLNHEISRVYPGHRMVNPHLCTVKLSRRVLPNLLNHRLDT
                     IASHFSIPIASRHRAGSDALATAEIFLLLMTRLEEIHGVKDLAGAREFQFEPKCENAA
                     REIPPAPLASSLQ"
     gene            1755..2345
                     /gene="pyrE"
                     /locus_tag="VFH91_09670"
     CDS             1755..2345
                     /gene="pyrE"
                     /locus_tag="VFH91_09670"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET6863307.1"
                     /translation="MKAEEVVQEFKDKGALLEGHFQLSSGLHSTVYLQCARVLQYPDI
                     AERFGQAIARNFEGQEIQLVASPAIGGLIIGHEVARALGARFVWTEREKGVMTLRRGF
                     SVSDGERTLVVEDVITTGGSTRETIEVLKAAGANVVGAASIIDRSKGDADVGVPRITL
                     ASLKVTSVEAANCDACRQGEPITKPGSKTQSVVSGN"
     gene            2357..3109
                     /gene="truA"
                     /locus_tag="VFH91_09675"
     CDS             2357..3109
                     /gene="truA"
                     /locus_tag="VFH91_09675"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET6863308.1"
                     /translation="MNYKLTLQYDGTDFHGWQIQTGLRTIQGELSAALSLIDGREITL
                     HGSGRTDAGVHAEGQVASVQLERTITADKLRAAINANVGKDLRVMEVASVDDDFHARY
                     SAVGKTYLYRILNGSVVSPFWIRYALQEARSLDLERMKSVANLFLGKHDWTAFSAAQS
                     DVEDRVRTITDLTINGSHDPRARQSLLEIRVSADGFLRYMVRAIAGTLLAVGRRELDA
                     VTVRDAIENGRRPLEAVTAPSHGLTLLEVRYK"
BASE COUNT          795 a          776 c          919 g          868 t
ORIGIN      
        1 tccgctacta tgttgaagga attctggctg ttatatacgg acgtagggtt ttgctttttc
       61 taagggataa cggccttgtt attatcagta tggtagcgac cattattctc ataggattgt
      121 tgatctatta tttcgttaat aagcgaaaag tagcggcgat tatggcggaa gccgatgacc
      181 agccactcga cgcttccccc gataccgaaa gggttattga ttgaagtcat ttattcatcc
      241 agttaccaaa gcgctgacac gtctgtctga gtatttagtt actttcgggg cgttcggcct
      301 ttttcttgtt gccgtcctcg actccacttt tgttccgtta ccaagcagcg tcgatgcgtt
      361 gatgcttctg ctgtcggcga caaatccgcg ttggatgctt gtttatgctt tgatggcgac
      421 ctccggttcg gcccttggct gctggatcct gtacgtgata tctcgtcgtg ctggcgcccg
      481 ggctcttcga cgattttccg cagcgaaaca acaacgagtc aaggatctaa tcgatcgcta
      541 cgacatgatg gcggtattgg tcgcaacgct attgcctccg ccgtttccat tcaagttgtt
      601 tgtcatatca gcaggcgttt ttcgctttag tccaactcgc ttcatgattg caattatcgg
      661 tggacgggca tttcgcttct tgctcgaggg atatttcgcc gttcgttacg gagcagaggc
      721 taaagtgatc ctggcaaagt actatccctg gataggggtt ggtttagccg tactgatact
      781 tgcgggcttt atagtgcgtc gtttactaag gaagagtcgc gaggtagtgg ttgaacagtc
      841 actggagact ggaagctaga ggcgcagggg gaatttctct cgcggcgttt tcacacttcg
      901 gctcgaattg gaactctcgc gctccagcta gatctttaac gccgtgtatt tcttcaagtc
      961 tggtcatcaa caacaagaag atttctgcag ttgcaagtgc gtcgctgccg gcgcggtgtc
     1021 gacttgcgat tggaatcgaa aaatggctgg cgatcgtatc cagccggtga ttcagtaagt
     1081 ttggaagtac tcgtcgcgac agcttgaccg tgcaaaggtg agggttaacc attcgatggc
     1141 cgggatagac acgcgagatt tcgtgattca aaaaactcgt gtcgaacggg gcattgtggg
     1201 ctatcaagac tgaatcgctt acgaaatcta accatcgtgg cgcaacatcg gcgaaaactg
     1261 gcgctcgttt gaccatgtcg ttcgagatgc cagtcagaga ggcgacaaat cttggaatcg
     1321 ggatttccgg attcaccagg gtcaaaaact tatcgacaat gcgaccgtct cgtattcgga
     1381 aagcaccaag ttcaatcagt cggttcggag gtgttttggc gccagttgct tcaacgtcca
     1441 cgacgacgaa ctctaagtct ttaagaagtc gccggcccca atcgtcctcg agcaattcaa
     1501 ttgtgtgatt atctccgatt cgaaaacgcc gatcattgcg aatcagatca gcaaccagca
     1561 ggctggcaag ttcgtcatcg atatgggaaa gtttgaaaac agcgtcaaca atgtgcgacg
     1621 cgggagctcg accgcccgaa tcgtttaaga ggtcaattgt gtcctgaacg agagtggaat
     1681 cagaaactag gttcggaaac ggcgacatgc gggcaccgat tgtagcacgc taatacattt
     1741 agcgaggtaa gaatttgaaa gcagaagagg tcgttcagga atttaaggat aaaggcgcgt
     1801 tgctagaggg tcattttcaa ctttccagtg gccttcacag caccgtctat cttcagtgtg
     1861 cccgggtcct gcaatatcca gatatcgcag aacgcttcgg tcaggcgatc gcccggaact
     1921 ttgaaggaca agaaattcaa ctagtggctt cgcccgcgat tggcgggctc attattgggc
     1981 acgaagtcgc ccgcgctttg ggtgcgcgat tcgtgtggac cgaacgagaa aaaggtgtca
     2041 tgacgttacg ccggggtttt tccgtatctg atggagaaag aactctggtg gtggaagatg
     2101 tgatcacgac tggaggttca actcgagaaa caatcgaagt gttgaaagcg gctggcgcaa
     2161 atgtggttgg cgcagcttcg attattgatc gatcaaaagg cgatgcggat gtcggcgttc
     2221 cgagaattac tctggcttct ctcaaagtca cttcagtgga agctgccaac tgtgacgcct
     2281 gtcgtcaggg cgaaccaata accaaacccg gcagtaagac acagtccgtc gtcagtggga
     2341 actgaccttc acgtcagtga actacaaact tacgctccaa tacgacggta cagatttcca
     2401 cggttggcaa atccaaacgg gactacgaac tatacaaggc gaactgtccg cagctctatc
     2461 gttgatcgac ggacgggaaa ttacactcca cggttcggga cgtacagacg cgggtgttca
     2521 tgctgagggg caggtcgcca gcgtccaact ggaacgaaca attactgctg acaagttacg
     2581 agcagcaata aatgccaatg ttggcaaaga cctgcgcgtg atggaggtag caagcgttga
     2641 tgacgacttt cacgcccgct attcagccgt tggaaaaact tatctctacc ggattctaaa
     2701 tggttctgta gtctctccgt tctggattcg ttacgcgtta caggaagcgc ggtcgcttga
     2761 tcttgaacgg atgaagtcag ttgcaaatct gtttctcgga aaacatgatt ggaccgcatt
     2821 ttcggcggct caatcagatg ttgaagatcg agtgcgtacg atcacagact taactattaa
     2881 cggttctcac gatcctcgtg cccgtcaatc tttgctggaa attcgagtca gcgctgacgg
     2941 atttcttcgt tacatggtgc gcgcaattgc gggcactctt ttagctgtag gtcgtcggga
     3001 attggacgct gtgactgtgc gcgatgcgat cgaaaatggg cgccgacctc ttgaggccgt
     3061 gacggcaccg tcacatggtc tgacgttatt agaagtaaga tacaagtgaa ttgatgagga
     3121 ttctgttgac agtctttgat aagcggacaa gaatgacagc gctttagctg aaggtagctc
     3181 ttcaaatttc gaggagccta aaaagcgccg gcgggctggc gcactccata tatgcttgct
     3241 gattttgctg gaatagttac gcctggaagt ctggatgagt cgttgctcgc gacaatcgcc
     3301 tgggatcgtt tgccgcgtca tgtcgcgatc atcatggatg gcaacggtcg ttgggcgg
//
LOCUS       DASSFP010000429         6491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_434129, whole genome shotgun sequence.
ACCESSION   DASSFP010000429 DASSFP010000000
VERSION     DASSFP010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434129"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFH91_09680"
     CDS             complement(<1..890)
                     /locus_tag="VFH91_09680"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HET6863309.1"
                     /translation="MCGIVGYVGNKQVVPLIIDGLRKLEYRGYDSAGIAVVNDSHNLE
                     IRRAEGKLRNLEETIRLNPIDGTYGIGHTRWATHGRPTEENAHPHRDCTGRVVVVHNG
                     IIENYLALKERLRKTDHRFVTETDTEIIAHLIEEYLKQGDSFEAAVRATVAELKGIFA
                     LSIINADEPDTIIAVRQGPPVVIGLGDREFFVASDIPPILQHTRDVFFLGDGEIAIIN
                     KEAVRVIDFEGNSVQPSIQRITWDPIMAEKGGFKHFMLKEIYEQPRSLRDTVQGRISL
                     DTGQVFLDEMANITEADFKR"
     gene            complement(917..2305)
                     /gene="glmU"
                     /locus_tag="VFH91_09685"
     CDS             complement(917..2305)
                     /gene="glmU"
                     /locus_tag="VFH91_09685"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET6863310.1"
                     /translation="MSEKNPSVKPLEVLILAAGLGTRMKSRRAKVLHELGGLTLIAHV
                     TRTALALSPQSILVVVGHQAEQVENAVLAEASDRARFAVQARQNGTGDAVESARDLLN
                     NSDSLLLILSGDVPLIKSETLQHFIEHHRTTAAACSILSVRLENPTGYGRIIRDDNGR
                     FEKIVEHRDATPEQRQMREINSGIYCFDTQKLFSALRHVKPANDQGEYYLTDVAEILK
                     ASNEPVDVYEHHDAREVSGINTRAELAEFENLLRRGTIRSLMVESGVTFIDPSHAYIS
                     ADARIGRDCVIHPGVTIEGRSIIGENCEIRYGSRISNSIIGDNVVIKDHSLIIDSEVG
                     SDCSVGPFAHLRMNSKLEDKARVGNFVEVKKSVLGRETKAMHLSYLGNATIGAKTNIG
                     AGTVTCNYDGVRKNETFIEDNVKIGADTMLVAPVRVGAGSVTGAGSVVTRDVPPDSLV
                     AGVPAELKKKLK"
     gene            complement(2327..3343)
                     /locus_tag="VFH91_09690"
     CDS             complement(2327..3343)
                     /locus_tag="VFH91_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET6863311.1"
                     /translation="MLSLSGQNLVAIGGGTGLSTLLAGLKKLVGVTSNEIWIDNLSAI
                     VTVSDDGGSSGRLREELQMLPPGDIRNCMIALSEDSSLLSRLFRHRFRGEGELGGHSF
                     GNLFLAALTEVTGDFTEAIRLSSDILASKGHIYPATISDVRLVAELEDGTVVRGETHI
                     SASSVPIRLLRLEPEQCLPLPQVLKAIRAADVITVGPGSLYTSILPNLLVSQVARVIG
                     ETQAIKIFINNLMTQPGETDGYTARKHLETVKKYAPEIHFDFVIVNDRRISKEQAELY
                     AAEDAYQIGIDDPIDEAVDQVTEVVRANLLDAGEKVRHNSDRLAQVVLSCLQRAYQRS
                     AVTA"
     gene            complement(3476..4327)
                     /locus_tag="VFH91_09695"
     CDS             complement(3476..4327)
                     /locus_tag="VFH91_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6863312.1"
                     /translation="MSSSLTKGLRRSSARPTAKRASAKTAKRVAKTASSLPKAKRATS
                     VKPASRKVTAGKKVTAKAKSAPARTKKPAAKAAPKPAARKTVVPKKAVAQRVKKTQKV
                     AAVRAIRAITPPPSPRPPTQDESAALVAFERAHKEFARGRFAEARNQFRSLLEKYSQV
                     SEVAARARTYLAIAESRLRTESILPRDADSLYDRGVIELNRGEYVAAQEMFERALKRE
                     PEAAHIHYGLAATRARLGSIDLALESLQRALDLQPNLRVRAQHDHDLNPLRNDPDFDR
                     LIFSNPS"
     gene            4680..5153
                     /locus_tag="VFH91_09700"
     CDS             4680..5153
                     /locus_tag="VFH91_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET6863313.1"
                     /translation="MADTPILLREQQYHQIKAVLARLRMDASAKVVFLVDKDGQEIAS
                     QGEVGNLDTTSLASLAAGNVAATGGMAQLIGEKEFPTLSHEGERESIHISVIGRLLLV
                     VVFDERSSLGLVKLRSKQISQQLASMFDEITSGDFDDLDSPFAGITDEDIDSLFS"
     gene            5165..5749
                     /locus_tag="VFH91_09705"
     CDS             5165..5749
                     /locus_tag="VFH91_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET6863314.1"
                     /translation="MTFINYASREINCKIVYYGPGLCGKTTNLQYIFDSTAPQARGKL
                     ISLATETDRTLFFDFMPLELGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQEERMDANIESLYNLEENLQSQGYDLMKIPYVLQLNKRDLGNIIPAEDLAAELQR
                     KGEPVVEAVASTGAGVFDTLKAVAKQVLTELRKG"
     gene            complement(5760..6311)
                     /locus_tag="VFH91_09710"
     CDS             complement(5760..6311)
                     /locus_tag="VFH91_09710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET6863315.1"
                     /translation="MAVLKKFKSRWWSQSDIEYDSEQKEKTVTPTWGLRYDVRPLTIS
                     QLDECWRLDQRCFIDGEAYSRETFDYLLTAPESVSYRAVTAGGVMVGFIIGLVEPDHT
                     GHVTTVGVSPEHRRRNLAKRLMAQVEEGFRRRHVRLVRLEVRALNTPAQKLYEGIGYS
                     ITQRLPKYYSNGGDGLLMLKSLD"
BASE COUNT         1509 a         1684 c         1665 g         1633 t
ORIGIN      
        1 aacctcttga aatccgcttc agtgatattg gccatctcgt caagaaacac ctgtccggtg
       61 tcaagtgaga tgcggccctg gacggtgtcg cgaagtgagc gtggctgctc gtagatttcc
      121 ttgagcatga aatgcttgaa gccacccttt tccgccatga tcggatccca ggtgattcgc
      181 tgaatagacg gctgtacaga gttcccctca aagtcaataa ctctgaccgc ctctttatta
      241 ataatggcga tctctccatc gccgagaaag aagacgtctc gtgtgtgttg caggattgga
      301 ggaatgtcgg atgccacgaa aaattcgcga tctcctaaac caataacaac gggcggtcct
      361 tgtcgcaccg cgatgatggt gtcaggctca tctgcattga tgatggataa cgcaaagatg
      421 cctttgagct cagccacagt cgcacgtacc gcggcttcga acgagtctcc ctgtttcaaa
      481 tactcttcaa tcagatgtgc aatgatttcg gtatcggtct cggttacaaa tcggtggtca
      541 gtttttcgca ggcgttcctt aagcgcgagg tagttctcga tgatgccatt gtggacaacc
      601 accacccggc cggtgcagtc gcgatgcgga tgtgcattct cttcagtagg acggccgtga
      661 gtggcccaac gggtatggcc aataccgtaa gtcccgtcga ttgggtttag tcgaatcgtc
      721 tcttcgaggt ttcgcagttt gccttcggcg cgacggattt cgagattgtg actgtcgttg
      781 acgaccgcaa tcccggcaga gtcgtaaccg cgatactcga gtttgcgcaa accatcgata
      841 attaacggta cgacctgttt gttcccgaca tatccaacga ttccacacat gttgttttgc
      901 tcaactcccc agtctgttat ttgagtttct tcttcaattc cgcaggaact ccagcgacta
      961 aagaatcagg cggaacgtcc ctggtcacca ccgagcctgc tccggtgact gaacctgcac
     1021 ctactcgaac cggagctacc aacatcgtgt cagctccgat ctttacgttg tcttcaataa
     1081 aagtttcatt tttgcggacg ccgtcgtaat tgcatgtgac ggtgccggct ccgatgttgg
     1141 tttttgctcc gatcgtcgca ttgccaaggt agctgagatg cattgccttt gtttcgcgcc
     1201 ctaagaccga ctttttaact tcaacaaagt taccaaccct cgccttgtcc tcaagcttcg
     1261 aattcatccg caagtgcgca aacgggccga ccgaacagtc cgagccaact tcggaatcga
     1321 ttattaaaga gtggtccttg attacaacgt tgtcgccaat tatcgaattt gatattcgcg
     1381 agccgtaacg gatttcgcaa ttttcaccga tgatcgagcg accctcaata gtcacgccgg
     1441 gatggatcac acagtctcga cctattcgtg catcggcact aatgtaagcg tgcgaaggat
     1501 cgataaaggt cacgccactt tcgaccatca gactgcgaat tgtcccgcgt cgcaaaaggt
     1561 tctcaaattc ggccaactca gctctcgtgt tgatgccgga gacttcgcgt gcgtcatggt
     1621 gttcgtagac gtctaccggc tcattactgg ccttaagaat ttcagcaaca tcagtcagat
     1681 agtactctcc ctggtcgttc gccggtttta catgacgcaa ggcggaaaac aatttctgag
     1741 tgtcaaagca gtaaatgccg gaattgatct ctcgcatctg ccgttgttcc ggggtagcgt
     1801 cgcgatgctc aacgatcttt tcaaatctac cgttgtcatc tcgaataatt cgaccatatc
     1861 cggtgggatt ctccagtcga acgcttaaaa ttgaacacgc cgccgcggtt gttcggtgat
     1921 gttcgatgaa gtgctgtaga gtttccgact ttatcaaagg tacatcaccc gacaatatca
     1981 acagcagaga atcagaatta ttcagcaggt ctcgagctga ttcaacggca tcacccgttc
     2041 cgttttgacg cgcttgcacg gcgaaacgcg ctctgtcact tgcttcagcc agtactgcgt
     2101 tctccacctg ttcagcctgg tgtccgacga ccacaagaat tgactgagga gataaggcga
     2161 gggcagtgcg cgtcacgtgc gcaatgagtg ttagtccgcc aagttcatga agtaccttag
     2221 cccggcgcga cttcatacga gttccgagac cagctgcaag tatcaatacc tccagtggtt
     2281 ttactgatgg gttcttctct gacaagtgtt tctccgtttt ttagtgctag gcggttactg
     2341 ctgagcgttg gtatgcgcgc tgcaggcagc ttagaaccac ctgcgcgagt cgatctgaat
     2401 tgtgtcgcac tttctctccc gcatcgagta ggttcgcgcg cactacttcc gttacttgat
     2461 cgactgcttc atcgattgga tcgtcgatcc caatctgata cgcatcctct gccgcgtaaa
     2521 gctctgcctg ttctttgctg atccggcgat cattaacgat cacgaaatcg aagtgaattt
     2581 ccggagcata ttttttgaca gtctcaaggt gtttcctggc ggtatatccg tcggtctcgc
     2641 cgggttgcgt catcaggttg ttaataaaga tttttatcgc ctgcgtttca ccgataaccc
     2701 gggcgacctg ggagacgagt agattcggca gaatactcgt gtataacgat cctggaccaa
     2761 cagtgatcac atcggcggct cggatcgctt ttaaaacctg gggtaaagga agacactgct
     2821 cgggctcgag tcgaagcagg cgaattggga ctgaagaggc ggagatgtgc gtttcacccc
     2881 gaacgacagt accgtcctcc aactctgcga cgagtctgac gtcgctaatt gtggccgggt
     2941 agatgtgacc tttgcttgca agaatatctg acgagagccg gatggcttcg gtgaagtctc
     3001 cagttacctc agtcaaggcg gcaagaaaca ggttgccaaa gctgtgccca ccaagttctc
     3061 cttcgcctcg aaatcgatgt ctaaaaagac gcgagagaag actcgagtct tcagacaggg
     3121 caatcatgca attgcggatg tcaccgggag gaagcatttg cagttcctcg cgaagacgtc
     3181 cggaactacc cccatcatcc gagacagtca caatcgcgga tagattgtcg atccagattt
     3241 cattggaagt aacacccacc agctttttta atccagcaag gagggtggag aggccagtac
     3301 ctccgccaat tgcaaccagg ttttgaccag acaaactcaa cattttttgc aattgaatac
     3361 ttactcccag ggaagcaaag ttccgtattg gacgggaatc ataacctaaa gacgcctttt
     3421 ctcaatccag gggcgctagt cattccaggg tcggattata gaacggatag ctcgttcagg
     3481 aggggttgct gaagatgagc cggtcgaagt cagggtcgtt gcgaagcgga ttgagatcat
     3541 ggtcgtgctg agctcgaacc cgaagatttg gttggagatc caatgcgcgc tgtaaagatt
     3601 cgagcgcgag gtcaatagag ccaagtcgag ctcgcgttgc ggcgagtcca taatgaatgt
     3661 gtgcggcctc aggctcgcgt tttaaggcac gctcaaacat ttcctgcgca gcgacatatt
     3721 caccgcgatt taattcaata acgccgcggt cataaagtga atcagcgtct cgcggcagta
     3781 tgctttcggt gcgcagccga gactctgcta tcgcaagata ggttcgggca cgggctgcaa
     3841 cttcagatac ctgggaatat ttttctaaca gcgatcgaaa ctgattgcga gcctccgcga
     3901 aacggccccg ggcaaattcc ttatgggctc gctcaaaagc cacgagagca gccgactcat
     3961 cctgtgttgg tggtcttggg gaaggtggtg gcgttatggc acgaatcgca cgaacggctg
     4021 ccaccttctg cgtctttttg actctttggg cgacggcctt cttgggcaca acagtcttac
     4081 gggcggcagg cttaggagct gctttggcgg ctggcttctt tgtcctggcc ggcgctgact
     4141 ttgcctttgc tgtgaccttc ttacccgctg tgactttcct gcttgctggc ttaaccgacg
     4201 ttgctcgttt tgcttttggt agggaagaag ccgttttggc tacgcgcttc gcggttttcg
     4261 ccgatgctcg cttggcagtc ggacgggcgg atgatcgacg caggcctttt gtgagactgc
     4321 tgctcatgtc cggtatctca aaaaggctgc tcaaccgtcg cgagcagccg aagaatgggc
     4381 ggagcttagc acatctgggc gttctaaatc aacgaacaca ataatttgcg gccttacgcc
     4441 cccaatacct tccctgctaa acactttaaa ggtaacaaca aagaaaaacc ttgatgggca
     4501 ttgttggcta tgttaaagtg cgtggtggct tacgaaggtc gatatgacct aggcgcaatg
     4561 ccccaaccga tttagtaacc tcttttactt tcaagatttg cgacccgcga agcggcggtt
     4621 cgggtttact ctccgattag aacattccca cccggccctc aaaaaaaaga gtcgtaacga
     4681 tggcagacac tccaatcctg ttgcgtgaac agcagtacca ccagataaag gcagtccttg
     4741 cccgactcag aatggatgcc tcggcgaagg tggtctttct ggttgataaa gatggccagg
     4801 agatcgcttc ccaaggtgag gtgggaaatc ttgacacaac tagccttgcc tctctggcgg
     4861 ccggaaatgt tgcggctacg ggtggcatgg ctcaactgat cggcgagaaa gagtttccga
     4921 ctctctccca tgaaggtgag cgggaaagta ttcacatctc cgttatcggc cgactcctcc
     4981 tggttgtggt tttcgatgaa cgctcgagct tgggattagt caaactccgc agcaagcaga
     5041 tatctcagca acttgcttcg atgtttgacg aaattacaag tggtgacttc gacgacctcg
     5101 actctccctt tgcaggcata accgatgaag acatcgatag cctattcagt tagcagcgtg
     5161 tgccatgacc tttatcaatt acgcttctcg agaaattaac tgcaagatcg tctactacgg
     5221 cccaggactg tgtggtaaga caaccaacct gcagtacatc ttcgactcca ctgctcccca
     5281 ggcgcgtggc aagctgatca gtcttgcgac cgaaaccgac cgaactttgt tcttcgactt
     5341 tatgcccctt gagctcggaa ccgttcgagg cttcaagacc cggttccatc tttacacggt
     5401 gccggggcaa gtattttatg acgcttcgcg aaagctcatc cttaagggtg ttgatggcgt
     5461 tgtgttcgtt gcagactctc aggaagagcg catggacgcc aatatcgagt cgctttataa
     5521 cctggaagag aacttacagt ctcagggtta tgacctgatg aagatcccct acgttttgca
     5581 gctcaacaag cgcgaccttg gcaatattat tcccgctgaa gacctcgccg cagagttaca
     5641 gaggaaaggt gaaccagttg ttgaagccgt agcgtctact ggtgcgggag ttttcgatac
     5701 tttaaaagcg gttgcgaagc aggtgttgac ggagcttagg aaaggttagc ctgacgcgac
     5761 tagtcaaggg acttcagcat tagaagacca tccccgccat tcgaataata tttaggaagc
     5821 ctttgagtaa ttgaatagcc aattccttcg tacagttttt gtgctggtgt gtttagcgca
     5881 cgaacttcga gacggaccag tctgacgtgt ctgcgacgga atccctcttc gacttgtgcc
     5941 atcaatcgtt tggccagatt gcgccggcga tgttcgggcg agaccccgac ggtggtgacg
     6001 tgtcccgtgt gatctggctc gaccaggcca atgatgaatc ccaccattac gcctcctgct
     6061 gtcacggccc gataagacac cgactcaggc gccgtcagaa ggtaatcgaa tgtctcccgg
     6121 ctatacgctt caccgtcaat aaagcagcgt tgatcaagtc tccagcactc gtccaactgt
     6181 gaaatcgtga gtggacggac gtcatagcgg aggccccaag taggggttac agtcttctcc
     6241 ttttgctcgg agtcgtactc aatgtccgac tgcgaccacc agcgcgactt gaatttctta
     6301 agtactgcca tagaaatttg atggggtaaa ctgccgacca agtttaccat cagcctttgc
     6361 gtcctgccta aagaattaac atgcaatcac catagctgta aaaacgataa cgatgttcga
     6421 ctgcgtgtcg ataggcctcg agaaccaggt ctcgcccggc aaatgcgcaa accaataaaa
     6481 gtagcgacga t
//
LOCUS       DASSFP010000430         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_869959, whole genome shotgun sequence.
ACCESSION   DASSFP010000430 DASSFP010000000
VERSION     DASSFP010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869959"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFH91_09715"
     CDS             complement(<1..562)
                     /locus_tag="VFH91_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863316.1"
                     /translation="MARPRPISVSVSRRLTIAIVSGLSLLALSFPTSVQRAATAPILL
                     TKINSTRAIAVESTNLVAEPFTATAPVPFAVDNRTRIILFSSNLSLANGETVSAVTAD
                     AEDGNHQHYPLTVEYVGTVSGFPWMTAVTVRLNDNLGDVGDVLIGISYAGLTSNRVRV
                     GIGHIGGGPPDDTPPPQPVPDLGLGAS"
     gene            739..1011
                     /locus_tag="VFH91_09720"
     CDS             739..1011
                     /locus_tag="VFH91_09720"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HET6863317.1"
                     /translation="MKFMVIEQFRGGDSTRVYARFQEHGRMLPDGLKYIDSWVDINSG
                     RCFQLMETEDPKLFDKWIGCWNDLVQFEIIPVVTSAEAAEQGPNKL"
     gene            1605..2282
                     /locus_tag="VFH91_09725"
     CDS             1605..2282
                     /locus_tag="VFH91_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6863318.1"
                     /translation="MKARLQNVAEDHQAGGRRAQGERTRQAILEAAVHIASAEGLEGL
                     TIGRLALELSMSKSGLFAHFGSKEDLQVATVETARAIFIREVIKPAFEASEGLLRLWT
                     LCDIWLGYVQNGIFRGGCFFAAAAAEFDGRPGVVRDRIAEIMKEWLSTLQRAVGDAQK
                     AGQLKPEVDASQLAFEFNSLELGANWEYQLYGDHRSFSRAREAINQRLTSYATAAGRR
                     LLAAKET"
     gene            2313..>2623
                     /locus_tag="VFH91_09730"
     CDS             2313..>2623
                     /locus_tag="VFH91_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863319.1"
                     /translation="MVASRLASENIRRSASWDSIRVEHWRFNGGELLAHSHREHTVLI
                     VLSDGAKGEFRTESGRGMCSRQAAQGSVAILPSGLEHSAVLDGPSEHLSLFIDPSLIT
                     RA"
BASE COUNT          628 a          643 c          719 g          633 t
ORIGIN      
        1 agctggcgcc tagtcccaaa tccgggactg gctgcggcgg tggcgtatcg tcaggtggcc
       61 caccgccgat atggcctatt ccaacacgaa ctctgttgct ggtgagtcct gcgtaactaa
      121 ttccgatgag tacatcgcca acatcaccca gattgtcgtt caatcgcact gtcactgccg
      181 tcatccaggg gaagcctgaa accgtaccaa catactcaac tgtaagcggg tagtgctgat
      241 ggttcccatc ctcagcgtcc gccgtcacag cagacactgt ctcgccgttt gcaagactga
      301 ggttgctcga aaagagaatg attcgagttc tattatctac tgcgaacgga actggagcgg
      361 ttgcagtgaa gggctcggcc accagattcg tggactctac cgcgatggcg cgagttgaat
      421 ttattttggt gagcagaatc ggcgccgtcg ctgcgcgttg gacactcgtc ggaaacgaga
      481 gggcaagcaa cgacaagccg gaaacgatcg caatcgttaa tcgccttgag actgagacgc
      541 taatgggacg aggccttgcc ataaggctcc aaaattggct ttagatgttg aggttagggc
      601 agttgaggaa agagcaagta cgagtcaaac ttaaatgggc caacggccga agtcaagatt
      661 ttcttgtcgt gctcatgtta ggtttttaac gatacgcaat tagtctcatg tactcggccg
      721 gtttgggagt ttaaagctat gaaattcatg gtcatcgagc agtttagagg tggggactcg
      781 acccgggttt acgcccgctt ccaggagcat ggacgcatgc tacctgacgg tttgaagtac
      841 attgacagtt gggtcgacat taactcgggt agatgttttc agctgatgga gaccgaagat
      901 cctaagctct tcgacaaatg gattggctgc tggaacgacc tggtccagtt tgagattatt
      961 ccagttgtga catcagccga ggctgcagaa caaggcccaa acaagctcta attttcagcg
     1021 ccgccacgtt gtggaagcat gtcacggtca tcagcgacgg cgttagtaac tggtgcatga
     1081 tttcaagatt acatcgctgc gatacgtgag cttgctaatt cggtctcgac ctggcccatg
     1141 cggaaagacg aaagcgtcta caaagatcta cagttgggcc gggatgctgt ctacattcct
     1201 ctacatcctt gtctccttgt ttaaccttca caggatacaa catagaaaat atgggccatc
     1261 tcagggttgg tatgttcatt gcctaggacg aggttcaaca acgattcgcg gcgaggatac
     1321 tcctaaacta cttgccgaga acctggtcgg agtgcggggg ccttatacct gatttggtca
     1381 cgcgtgcaac acgagcttct gggcaaatta atagtttcgt gagtcacgcg ggaattacat
     1441 agatcaaagc tctgtgaggt ctccatcccc gccgggcttt tagtctccgg cacactgcaa
     1501 ctcttgatgt aaaacgaacg ttcgtgctat atagagaagc tgctcgctga tgaagcattg
     1561 agaaagctta ctgtcaaaac aatttcggaa gtggcaatca tttcgtgaaa gctcgtttac
     1621 aaaacgttgc agaggatcat caagcaggtg ggcggcgggc ccaaggcgaa cgtacgcgcc
     1681 aggctatcct cgaagcggct gttcatattg cctctgccga gggactcgaa ggactaacca
     1741 tcgggcggtt ggctcttgaa ctttcaatga gcaagagcgg tctctttgcg cattttggct
     1801 ccaaggaaga tctacaggtc gcaactgtcg aaactgcgcg cgcgatcttc atcagagaag
     1861 tgatcaaacc cgcattcgaa gcttctgaag gtctccttcg cctttggaca ctctgcgaca
     1921 tctggttggg ttacgttcaa aatggcatct ttcgtggtgg ctgtttcttc gctgctgcgg
     1981 ccgcagaatt cgatggccga cccggggtcg tccgcgaccg gatcgccgaa ataatgaaag
     2041 agtggttatc aaccttacag agggcggtcg gagacgcgca gaaagcagga caactaaagc
     2101 ccgaggtaga cgcaagtcaa ttggcttttg agttcaattc actcgaactc ggcgcgaatt
     2161 gggagtacca gttgtatggc gatcatcgat cattcagccg tgctcgcgaa gccataaacc
     2221 aacgcttgac ttcttatgca accgctgccg gtcgccggtt gttagcggca aaagaaacct
     2281 agacttcagc tcaactgcat ttggaccaac ttatggttgc cagcagattg gcttctgaaa
     2341 atattcgacg cagtgccagt tgggattcca ttcgcgttga gcactggcgc tttaatggtg
     2401 gtgagttact tgcacatagt caccgtgaac atactgtatt gatcgtactg agcgatggcg
     2461 cgaagggtga gttccgtacg gaaagtggtc gtggcatgtg cagcagacag gcggcccagg
     2521 gaagtgttgc aattcttcct tccgggctcg aacactcagc cgtgttggat ggaccatcgg
     2581 agcatctttc gctattcatc gacccgtcgc tgatcacgcg cgc
//
LOCUS       DASSFP010000431         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1159289, whole genome shotgun sequence.
ACCESSION   DASSFP010000431 DASSFP010000000
VERSION     DASSFP010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159289"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VFH91_09735"
     CDS             <1..932
                     /locus_tag="VFH91_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET6863320.1"
                     /translation="GAEKFLKDGVTLPEGSLEKLSSEFDAILAGAFGDPRVPSNKHAE
                     DILLGMRRGLDLYINLRPVRLLDSRLTPLSNRKTEEIDFVVFRENTEGAYCGAGGFLK
                     HGTVDEIALQEEINTRRGVERIVVAAFEYARSTKRKKVTMADKSNVQRFGGDLWQRVF
                     KEVAAGYPEITANHQYVDAMAMFMVLDPAQYDVIVSNNLFGDILTDLGAAIQGGLGLA
                     ASGNIHPGKVSLFEPVHGSAPPLAGKGIANPVGAILTSAMMLEYLGFPAQSREIEEAV
                     RAAVSAKETTKDLGGILSTEEAGKAIVARVGKN"
     gene            948..1676
                     /locus_tag="VFH91_09740"
     CDS             948..1676
                     /locus_tag="VFH91_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863321.1"
                     /translation="MASGARTLATKAIAIVIIVALLDAVGVPPVIMMMVTGAVLIVWL
                     FARHTQSREVEKLFDFYVAADAILREEERRWYAFEISEVISEGERSLEAIADPPPLHL
                     FVLASLYYRLGEHKAAVEYLSRVVEDERYDELHRAVPSPQLRRYVSMLRRIEDEPSIA
                     PQALAAVRNLERARKKHARELLLESRERLEGDQQLEQMAAAIIEAKTSEAAKEQQSGS
                     QPLSAPPPISEVLHNVYDEQSPSN"
     gene            1694..2938
                     /locus_tag="VFH91_09745"
     CDS             1694..2938
                     /locus_tag="VFH91_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET6863322.1"
                     /translation="MFESLTAEIIAIGSEMLAPDRSDTNSLWLTEQLNRLGIEVKLKT
                     IVGDDDARLEETIRDAVRRSKVVISTGGLGPTEDDITRKITARALGRRLMLDEKVLEE
                     LRERFKGFGVVMPERNSRQAMVIEDAEILPNPNGTAPGMFIDHAGTAIVLLPGPPREM
                     KPMFENHVAHRLAGRAGSMRVLRRVLRVSGLGESAVDEKIAPIYTRYDNPVTTILFNQ
                     SEIEIHLTGRGRTDAEAESLIDKLAVEIEAALGDAVFSFAGETMEEVVGLKLSIGNYT
                     VSVAESCTGGLLAQRLTAIPGSSKYFIEGVVAYANEAKIRTLGVEPILLLEHGAVSAE
                     VAEAMAEGIRKVAGTDFGVSITGIAGPGGGTEDKPVGTVYIGLSYAAGTEHRKLKLPG
                     DRNLIRWRASQAALDLLRRRLL"
     gene            complement(2944..>3263)
                     /locus_tag="VFH91_09750"
     CDS             complement(2944..>3263)
                     /locus_tag="VFH91_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET6863323.1"
                     /translation="LLQELNQSKDVETVVVFVKNTGGESIFDYSLDLANHWRIGSNGR
                     GVLFLLSIDDRKWFIQVTRKLESVLTDHVCKAIGDKSTGFFKKQEYSAGVESFVKDLA
                     SKL"
BASE COUNT          818 a          766 c          895 g          784 t
ORIGIN      
        1 ggggagctga aaagtttctc aaggatggtg tgacgctacc cgagggaagt ctcgaaaaac
       61 tatcctctga attcgatgcg attcttgccg gagcttttgg cgatccacgt gttccgtcga
      121 acaaacatgc cgaagacatt cttttgggca tgcgacgcgg actggatctg tacatcaatc
      181 tgcgacctgt gcgactgctt gattcacgtc tcacgccttt atccaaccga aaaactgaag
      241 aaatcgactt cgtagtcttt cgagagaaca ccgaaggtgc ttactgcgga gccggtggct
      301 ttctaaaaca tggaaccgtc gacgagattg cactgcagga agagatcaac acgcggcgcg
      361 gtgtggagcg aatcgtcgta gcagcttttg agtacgcccg ttccactaaa aggaagaagg
      421 tcacgatggc ggacaagtcg aacgttcagc gctttggagg cgacttgtgg cagcgagtat
      481 ttaaggaagt agctgccggc tatcccgaga tcactgctaa tcatcaatat gtcgatgcaa
      541 tggcgatgtt catggtgctc gatcccgctc aatacgacgt gatcgtgagt aacaatcttt
      601 ttggcgacat tctgacggat ctgggagctg cgattcaggg aggactcggt ctggcagcgt
      661 caggcaacat tcatccagga aaagtttctc tatttgaacc agttcatggc agtgcgcctc
      721 cactggcggg aaagggaatc gccaatccgg ttggtgctat tctgacttca gcgatgatgt
      781 tagaatacct gggcttcccg gcacagagta gagaaatcga agaggcagtt cgcgctgcgg
      841 tttctgcaaa ggaaacgacc aaagatctcg ggggaattct ctcgacggag gaagcaggaa
      901 aagcgatcgt tgcgcgagta gggaagaatt aactgtgggg aggttttatg gcttcgggag
      961 caaggacact cgcgactaag gcgattgcta tagtgattat tgtagccctg ttagatgctg
     1021 taggagttcc gccagtgatc atgatgatgg taactggcgc agttcttatc gtttggttgt
     1081 ttgcccgtca tacccagtct cgtgaagttg agaagttatt tgatttttac gtggcagctg
     1141 acgcgatcct tcgcgaagaa gagcgacgtt ggtacgcgtt tgaaatcagc gaggtgattt
     1201 cggaaggcga gcgcagcctg gaagcgatag ctgatcctcc gccactccat ctctttgtgc
     1261 ttgcctccct ctactatcga ttgggagagc acaaggcagc cgtcgagtat ctttcgcggg
     1321 ttgtggaaga tgagcgttat gacgagctgc accgggccgt accatcaccg cagttgcgac
     1381 gatatgtttc gatgctgcgc agaattgaag atgagccatc aattgcaccc caggcattgg
     1441 ccgccgtcag aaatttagag cgtgctcgta aaaaacatgc tcgcgaacta ctgcttgaga
     1501 gtcgtgagcg gcttgaaggc gaccagcaac ttgagcaaat ggccgcggca ataattgaag
     1561 cgaaaacgag cgaagcagcg aaagaacagc agtcaggatc gcagccgctt tccgctccac
     1621 caccaatttc ggaagtcttg cataacgttt acgacgaaca gtctcccagt aactaaccaa
     1681 aatagagtct cgcttgtttg aaagcctaac agcagaaatc attgcgatcg ggtcggaaat
     1741 gcttgctccc gatcgcagtg acaccaatag tctctggctc accgaacaac tcaaccgcct
     1801 cggaattgaa gtaaagctca aaacaattgt cggtgacgac gacgcgcgtc ttgaagagac
     1861 gattcgcgat gccgtgcgtc ggtcaaaggt cgttatttca accggcggcc ttggtccaac
     1921 ggaagatgac atcaccagga aaattaccgc tcgggccctg ggccgtcgct tgatgctgga
     1981 tgagaaggtt cttgaggaac ttcgcgagcg atttaaaggc ttcggtgttg taatgcctga
     2041 gagaaactcg cgtcaggcaa tggtgattga ggatgccgag attttgccga atccaaacgg
     2101 cacagcgccg ggcatgttta tcgaccatgc gggaaccgca attgttctgt tgcctgggcc
     2161 accacgcgaa atgaaaccaa tgtttgagaa tcacgtggca catcgtctcg cgggtcgggc
     2221 gggcagcatg cgagtgctac gaagagtttt gcgtgtttcc ggtctgggtg agtcagcagt
     2281 cgatgaaaag attgcaccta tctataccag gtacgacaat cctgttacta caattctctt
     2341 taatcagagt gaaatcgaaa tccatctgac tggtcgtggg agaacagatg ctgaggctga
     2401 gagtctcatc gataaattgg cggtcgaaat cgaagcagcc cttggagatg cagttttctc
     2461 ttttgccggc gaaacaatgg aggaagtagt aggtttaaaa ttgtccatcg gtaactatac
     2521 cgtttcagta gctgaaagtt gcaccggtgg tctattggcc cagcgattga cggctattcc
     2581 cggatcatcg aagtacttca tcgaaggtgt ggtggcttat gccaacgaag cgaagattcg
     2641 aactttgggg gtcgagccaa ttttgttgct tgaacacggt gcagtgagtg cagaagttgc
     2701 cgaggccatg gccgaaggga ttcgtaaggt tgccgggaca gactttggtg tgtcgattac
     2761 cggcatcgct ggtccaggtg gcggaacgga agacaagccc gtgggcacag tctatatcgg
     2821 tttgtcatat gctgcaggaa ccgaacatcg aaagttgaag ctgcccggcg atagaaatct
     2881 cattcgttgg cgcgcctcac aggcagccct ggatttgctg cgaagacgac tgctttagct
     2941 ccttcaaagt ttgctcgcca agtcttttac gaaagattcc actccggcac tatattcttg
     3001 ttttttgaag aatcctgttg acttatcgcc aatcgcttta cagacgtggt cagtcaaaac
     3061 actttcaagc tttcgggtca cctgaataaa ccactttcga tcatcgatag acagtagaaa
     3121 tagaacgcct cgcccgtttg aaccgatcct ccaatgattt gccaaatcga gtgagtaatc
     3181 gaagatcgac tcaccgcccg tattctttac gaaaacaaca acagtctcga catccttact
     3241 ttgattgagc tcctgcagca att
//
LOCUS       DASSFP010000432         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_870472, whole genome shotgun sequence.
ACCESSION   DASSFP010000432 DASSFP010000000
VERSION     DASSFP010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870472"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="VFH91_09755"
     CDS             <1..1897
                     /locus_tag="VFH91_09755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET6863324.1"
                     /translation="AAPDFENTIVALEEAGQELNRVSTLYGVWSSTMNGPEFQVVQRE
                     MAPRLAAFNDRITQNELLFKRIEAVYNSPSKNKLNPEQQRLTWLYYTNFVRSGARLAP
                     EAKARLSEINQQLAGLYTKFSQNVLAEETDQFIVLKSEDELAGLPQSIRDAAAAAATT
                     KKQTGWVIMNTRSSIDPFLTYSDRRDLREKAWRMFVNRGDNGDEHDNNAIITEILQLR
                     AERAKLLGFKTHAHWRLENSMAKTPERAMELMEAVWKPAVARVHEEVADMQALADKEG
                     AKLKIEPWDYRYYMEKVRKAKYDLDQNEVKPYLQLEKLREGIFWVAGELFNFNFTPVT
                     NVPVAHPDIRVWEVTDKTTKRHIGLWYFDPYARAGKRSGAWMNAYRSQERVNGEITTI
                     VSNNANFVKGKPGEPLLISWDDATTMFHEFGHALHGLNSNVTYPSLAGTAVARDYVEF
                     PSQLMEHWLSTPEVLQRFALHYQTGKPIPQALVDKINRSATFNQGFATVEYLSAALVD
                     MKLHLAGDRKIDPDAFERETLAELGMPKEIVMRHRTPQFLHIFAGDSYSAGYYSYLWS
                     DVLTADAFGAFVEGKGPYDKAVAERLRKYVFSVGNTVDPADGYRAFRGRDPKIDALMK
                     KRGFSPS"
     gene            complement(1949..>2167)
                     /locus_tag="VFH91_09760"
     CDS             complement(1949..>2167)
                     /locus_tag="VFH91_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HET6863325.1"
                     /translation="WQNDALQFVISVVGVLVFTGLTAWDAQRLKQMAVSTPDGHTASL
                     AIVGALALYLDFINLFFFLLRFTGNRRS"
BASE COUNT          569 a          538 c          567 g          493 t
ORIGIN      
        1 agcggcgcct gacttcgaaa atactattgt tgctctcgaa gaagccggcc aggagttgaa
       61 tagagtctca actttgtacg gcgtttggag ctcgactatg aatgggccgg agtttcaagt
      121 ggtccaacgc gaaatggcgc cccgccttgc tgcgttcaac gaccggataa cccaaaacga
      181 acttctcttc aaacgaatcg aggccgtcta taactctccg tccaagaaca aactgaatcc
      241 agagcagcaa cggttaacgt ggctctatta caccaacttt gttcgatctg gagcgcgtct
      301 cgctcctgaa gcaaaagctc gtctatcgga gataaatcaa cagcttgccg gtctctacac
      361 aaaattcagt cagaatgttc tggcagaaga gacggaccag ttcatagttc taaagagcga
      421 agacgaatta gcgggactgc cccaatcaat tcgcgatgct gcagccgccg ctgcaacgac
      481 aaaaaaacaa actggttggg tgatcatgaa cactcgatca tcaatcgatc cgttccttac
      541 atattctgat cggcgtgatc ttcgcgagaa ggcgtggcgc atgtttgtca atcgcggcga
      601 caacggcgat gagcacgaca ataacgccat catcaccgag atccttcaac taagagccga
      661 gcgcgcaaaa ctattgggct tcaaaacaca cgctcactgg cgattggaaa actcgatggc
      721 caagactcca gaacgagcaa tggagttgat ggaggccgtt tggaaacccg cagtggcgcg
      781 tgtccacgaa gaagtcgccg acatgcaagc cctggctgac aaagaaggtg cgaagctcaa
      841 aattgaacca tgggattatc gttattacat ggagaaggta cgaaaagcga aatatgatct
      901 cgaccaaaat gaggtcaagc cgtatctgca actcgagaaa cttcgtgaag gaatcttttg
      961 ggttgcgggc gagctcttca acttcaactt tactcctgta acgaatgtac ccgtcgcgca
     1021 tccagacatt cgcgtttggg aagtgaccga taaaacaact aagcgacata ttggattgtg
     1081 gtatttcgac ccttatgcgc gagcagggaa acgttcaggg gcatggatga acgcttaccg
     1141 gtcgcaggaa agagtaaacg gcgaaatcac gaccatcgtt tcgaacaacg ctaactttgt
     1201 taagggcaaa cccggtgaac ctcttctgat ctcgtgggat gacgctacta caatgttcca
     1261 cgaattcgga cacgcgcttc acggtctgaa ttcaaatgta acgtacccgt cgttggcggg
     1321 tactgcggtc gctcgtgatt acgtagagtt tccttcacag ttgatggagc attggctttc
     1381 aacgcctgaa gttttgcaac ggttcgcgct gcactatcaa accggcaaac cgattcctca
     1441 agctctggtc gacaagatca accgatcggc gacattcaac cagggattcg cgacggttga
     1501 gtatctctca gccgcgctcg ttgacatgaa attgcatctc gcaggggatc gaaagattga
     1561 tccagatgca tttgaacgcg aaactctggc ggagcttggt atgccgaaag agatcgtcat
     1621 gcgccatcgt actccgcagt ttctccacat ttttgcgggc gattcttact cggctggcta
     1681 ttacagctac ctctggtcag atgtgttgac ggcggatgct tttggcgcgt ttgtggaagg
     1741 aaagggtcct tacgataaag cggttgctga gcggctgcgg aagtatgtgt tctctgttgg
     1801 gaatacggtc gatcccgcag acggttatcg cgcatttcgc ggccgcgatc ccaagataga
     1861 tgcgctgatg aaaaagcgag gctttagtcc gagttaagtt gggaaagttt atgcggggac
     1921 agatatcgtt tctgtccccg cgaggaattt agctgcgcct gtttccggtg aacctcagga
     1981 ggaagaagaa caggttaata aagtccaggt acagcgcgag cgctcccaca atagcaagcg
     2041 acgctgtgtg accatctgga gttgagactg ccatctgctt cagtcgttga gcatcccagg
     2101 cagtcaatcc ggtgaagacg agaactccca ccactgatat cacaaactgc agcgcatcgt
     2161 tctgcca
//
LOCUS       DASSFP010000433         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_565187, whole genome shotgun sequence.
ACCESSION   DASSFP010000433 DASSFP010000000
VERSION     DASSFP010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565187"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFH91_09765"
     CDS             complement(<1..558)
                     /locus_tag="VFH91_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6863326.1"
                     /translation="MRLGDYRIEIIPDTEFHLDGGAMFGVVPRNLWSEVCPPDDQNRI
                     RMNMNCVFVDTGSDKILIETGIGEKWQPKHSAMYGIERKHSLAETLKTKTNVTADEIT
                     IVVNTHLHFDHAGGNTELDQNGQAVASFRNARYFVSRSELAHADSPSERDRASYLSDN
                     WKPLQQTGQLEVKDDDYEVVPGLRME"
     gene            complement(715..1983)
                     /locus_tag="VFH91_09770"
     CDS             complement(715..1983)
                     /locus_tag="VFH91_09770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6863327.1"
                     /translation="MNSTKKALIAAAVALVLSAGLIFWQVKARKLGPVELTPEDMTLI
                     AEDQSPQLRMQLASDEAARKDFAKDVRRLLAVAEEARANNVDKTPEMKRQLEFQRASV
                     MAQYYFEHAGDKGADITDKEVEEFFKTPANQQRFDQLIADARAKNPQLKTQEIPKEQL
                     DQLKQRLGRIYIAEGKAKEQGFDKKPEVRVQILLQEARALAQEYAKDKLEEKMKATDD
                     EVNAYLASHPELDTDKKKREQAEEILKRARAGEDFAKLAQEYSTDGSKEKGGDLGWFG
                     HGDMVPEFEQAAYALKPGEISNVVQSKFGYHIIKLEERKTETKDGKPVEKIHARHILI
                     GDPNANPFAPPTSARDKAKAAVEQEKAEKVLDQIVARSQVKVPDTFQVKMPEQQPMPQ
                     GLPPGFGAPPSSGQPQPQPQKEPAPAKPHK"
     gene            complement(2004..2399)
                     /gene="mce"
                     /locus_tag="VFH91_09775"
     CDS             complement(2004..2399)
                     /gene="mce"
                     /locus_tag="VFH91_09775"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET6863328.1"
                     /translation="MKIDHIGIATKSLEEGLAVWRDALGLKVDSTEEVTEQGVRVAMM
                     AVGDTYVELLEALSPDSAVGKFIAKRGPGIHHIAIEVKDIRASLANLKDKGVRLIDEM
                     PRVGAGGCLVAFVHPSTTNGVLLELVQHQ"
     gene            complement(2452..3480)
                     /gene="meaB"
                     /locus_tag="VFH91_09780"
     CDS             complement(2452..3480)
                     /gene="meaB"
                     /locus_tag="VFH91_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET6863329.1"
                     /translation="MKELLKSSGSSLLAKFCDNSEIVYEMAVQIDSLGERVSKGDAAA
                     VARAISKIEDGSPDAAALMKHIYQQSRGGLVVGITGAPGAGKSSLVDKLALHYRRDGQ
                     RVGIVAVDPSSPFSGGAILGDRIRMQTLGLDEGVFIRSMATRGNLGGLARATVDAVAI
                     LDAAGYSKIIVETVGVGQDEVEIVKAADVSVVVLVPGMGDDIQAIKAGIMEIGDIFVI
                     NKADREGVYSTEKELEALLSLAMRDDGWNPPIVKTVATEGVGISDLVSAVESFVQAQQ
                     ASKISGERRRAIARWRIVELLRERLLNKVLESGSSVSTLDRMAEEVASRKRDPYSAVE
                     EIIQSSRN"
     gene            complement(3419..4564)
                     /locus_tag="VFH91_09785"
     CDS             complement(3419..4564)
                     /locus_tag="VFH91_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6863330.1"
                     /translation="MDFELNEEQQQIKMSVREFAQSEIAPHVMEWDESQHFPIELKPK
                     LAELGFLGVIFPEEYGGAGMGYVEYATIIEELSRVDGSVGISVAAHNSLCSNHIYQYG
                     TEEQKQKYLVPLASGKHLGAWGLTEPSAGSDASGTKSTAVPTDGGWIVNGSKNFITHA
                     IHGDTCVAMAVTDKEKRSKGISAFVFEKGMQGFSPSKKENKLGLRASETASVVFEDCF
                     VPADNMLGDEGQGFVNAMEILDGGRISIAALAVGIAQGAYESAVRYAKERQQFGKPIA
                     EFQAIQFKLADMATKIDAARLLMYRAASMKDNGKKTTKESSMAKLYASEISVSVCEEA
                     IQVHGGYGYTKDYPPEKYWRDSKLCTIGEGTSEIQRIIIAREVLRQL"
BASE COUNT         1041 a         1288 c         1051 g         1242 t
ORIGIN      
        1 ttccatccgc aggcccggaa ctacttcata atcgtcgtcc tttacttcca gttgacccgt
       61 ttgctgcaaa ggcttccaat tgtcgcttag ataactggct cgatcgcgtt cgctcggact
      121 gtcggcatgc gccaattcag accgagaaac aaagtatcga gcattgcgaa agctggcaac
      181 tgcttgccca ttttgatcaa gctcagtatt accaccagca tgatcaaaat gcagatgagt
      241 attgacgacg atggttatct catcggcggt cacattggtt ttcgttttca atgtttctgc
      301 cagcgaatgc tttcgctcaa tgccgtacat tgcagagtgt tttggctgcc atttctcacc
      361 aatgccagtc tcgatcagga tcttgtctga ccctgtatca acaaacacgc aattcatatt
      421 catgcgaatg cgattttgat catcaggagg gcaaacttcc gaccagagat tccgagggac
      481 cacaccgaac atggcgccac cgtccaaatg gaactcggtg tctggaatga tttcaattcg
      541 ataatcgcct aaacgcatga cggtggtccg tggtccgtgg tccgtggtca actagtaatt
      601 gttacgtgtc ccggattcca actcatttca gactcaagtc tcaaatcgat ctcaagatca
      661 cacatactaa aacaacggac tacggacaac ggactacgga ccacttacca gtttctactt
      721 atgtggtttc gctggtgcgg gttccttttg aggctgcggc tgcggttgac cactcgacgg
      781 cggagctcca aatcctggag gtaggccctg tggcatcggt tgctgctcag gcattttcac
      841 ctggaaagtg tccggcacct ttacctgcga acgcgccact atttggtcca ataccttctc
      901 ggccttttct tgttccactg ccgctttcgc cttgtcgcgt gctgacgtcg gaggagcaaa
      961 aggattcgcg tttggatcgc caatcagaat gtgacgcgcg tgaatcttct ctaccggctt
     1021 gccgtccttt gtctcagtct tacgctcttc cagcttgatg atgtgatatc caaatttact
     1081 ctgtacgacg ttactgatct caccgggctt tagagcgtag gcagcttgtt caaattccgg
     1141 caccatatcg ccatgtccaa accagcctaa gtctccgccc ttttctttgc tgccgtcggt
     1201 tgaatattcc tgcgccagct ttgcaaagtc ttcgccggct cttgctcgct tgagaatttc
     1261 ttctgcctgc tcgcgcttct tcttgtccgt atcgagctcc ggatgacttg cgaggtacgc
     1321 gttaacctcg tcgtctgttg ctttcatctt ctcctcgagc ttgtctttgg catattcctg
     1381 agccaatgct cgagcttcct gaagcaggat ctggaccctg acttctggtt tcttatcaaa
     1441 accctgttcc ttcgcttttc cttctgcaat atagatacgc cctaaacgct gtttgagctg
     1501 atcaagttgc tctttgggaa tctcctgagt tttcagctga ggattcttag ctcgagcatc
     1561 tgcgatgagt tgatcaaatc tttgctggtt ggcaggagtt ttgaaaaact cctcaacttc
     1621 cttgtctgta atgtcggctc ccttgtcacc agcgtgctca aaatagtact gcgccataac
     1681 gcttgcacgt tgaaactcga gctggcgctt catctcgggt gtcttgtcga cgttgtttgc
     1741 gcgagcttct tccgctactg cgagtaggcg gcgcacgtcc ttcgcaaagt ccttgcgggc
     1801 tgcttcatca gaagcgagtt gcattcttaa ctgcggagac tggtcttcag caataagagt
     1861 catatcttct ggggtcagtt caactggccc cagttttctg gctttgacct gccagaaaat
     1921 cagaccggca gagagaacaa gcgcgacggc tgcggcgata agtgcttttt tagttgagtt
     1981 caaaataact tcctttctta aacctattga tgctgcacga gctcgagcaa caccccgttt
     2041 gtggtggacg ggtgtacaaa ggccaccagg cagcctccag ctccaacccg tggcatctcg
     2101 tcaataagac gcacgccctt gtcttttaag ttggcaagtg acgcacgaat atctttaact
     2161 tctatagcga tgtgatgaat tccgggacca cgttttgcaa taaatttacc gacagctgaa
     2221 tcaggactga gcgcctcgag caattcgacg tatgtgtcac caactgccat catcgccaca
     2281 cgtacaccct gctcagtaac ttcttctgta gaatcgacct ttaagcctaa cgcgtcgcgc
     2341 caaaccgcaa ggccctcttc caggcttttg gtagcgatac cgatgtgatc aatcttcata
     2401 actaaccaat gttgctttgc gaaatcgggg agaacgccgc ccgcgtggct gttaatttct
     2461 cgaggattgg attatctcct caacggcaga ataagggtct cgcttccggc ttgcaacttc
     2521 ctccgccatc cgatcgaggg ttgaaactga tgaacctgat tcaagaactt tattaagcaa
     2581 tctttcccgc agcaactcca ctattcgcca ccgtgcaatt gcacgacgtc gctcaccact
     2641 gatcttgctt gcctgttgtg cctgaacaaa actctcgacc gcacttacga gatcggaaat
     2701 accaactccc tcagttgcga ctgtcttcac aatgggagga ttccacccgt cgtcgcgcat
     2761 tgccagtgaa agcagcgctt ccaattcctt ttcggtcgag taaactcctt cgcggtctgc
     2821 tttattaatg acaaagatgt caccaatctc cattatgccc gctttaatcg cctgaatatc
     2881 gtcgcccatt ccgggcacga gcactacaac tgagacatca gcagccttaa cgatttcgac
     2941 ttcgtcctgg cctaccccga cagtttctac aataattttg gagtaaccag cagcatcgag
     3001 aattgcaacg gcatcaactg ttgcgcgggc caggccgccg aggttaccac gcgtggccat
     3061 cgagcgaata aaaactcctt catcaagacc cagcgtttgc attcgaatcc gatccccgag
     3121 gatggcacca cctgagaatg gactcgatgg atcaactgca actattccaa cgcgttgacc
     3181 atcacgtcga taatgcaaag ccaatttatc aacgagcgat gatttcccag cacccggtgc
     3241 tccggtaatg cctacgacaa gtccgccacg tgactgctga taaatatgtt tcatcagggc
     3301 agcggcgtca ggcgaaccgt cctcaatctt cgatattgcc ctcgcaacgg cagcagcatc
     3361 acctttgctg acccgttcac ccaggctgtc gatctgcacg gccatctcgt aaacaatctc
     3421 agagttgtcg cagaacttcg cgagcaatga tgatccgctg gatttcagaa gttccttcac
     3481 caatagtgca gagcttggag tcgcgccagt atttttcagg cggatagtcc ttggtataac
     3541 cataaccgcc gtgaacctgg attgcctcct cacacacgct cacacttatt tcagacgcgt
     3601 acagcttggc cattgatgat tctttagtcg tcttctttcc gttgtctttc atcgatgcgg
     3661 cgcgatacat caacaatcga gccgcgtcaa ttttcgtagc catgtcagcc agcttgaatt
     3721 ggatcgcctg aaactccgcg attggtttgc cgaactgttg tcgctccttt gcataacgca
     3781 ccgcggattc gtaagcgccc tgggcaattc cgacggcaag agcggcaatt gaaatgcggc
     3841 caccatcaag tatttccatc gcgttcacga agccctggcc ttcatcgcca agcatattgt
     3901 ctgcaggaac gaagcaatct tcgaagacga cgctggcggt ctcggaagct cgcaacccta
     3961 acttgttctc cttcttggaa ggcgagaagc cctgcattcc tttttcaaac acaaaagctg
     4021 agatgccttt actgcgcttt tctttgtcgg tcactgccat tgccacacaa gtgtcgccgt
     4081 ggatagcgtg agtgatgaag ttcttcgagc cgttaactat ccaacctcca tcagtgggta
     4141 cagcggttga ttttgttccg gacgcatccg agcctgcact tggttcggtc aacccccagg
     4201 ctcctaaatg tttgccgctt gcaagtggaa ccaggtactt ctgcttttgc tcttctgtgc
     4261 cgtattgata gatgtgattt gaacaaagcg aattgtgagc cgccacagaa attccgacag
     4321 aaccatcgac acgagatagc tcttcaatga tggtggcata ctcgacatag cccatacccg
     4381 cgcccccata ctcttccggg aagataacgc cgagaaaacc aagttcagcg agtttgggct
     4441 tgagctcgat gggaaaatgc tgggattcat cccattccat tacgtgaggg gcgatttcgg
     4501 actgagcaaa ctcgcgaacg ctcatcttga tctgttgctg ctcttcgttt aactcaaaat
     4561 ccataaagtt tgttccttac acaacgttgg cattcctaac tgtccaagtc agccgacaga
     4621 ct
//
LOCUS       DASSFP010000434         4234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_277986, whole genome shotgun sequence.
ACCESSION   DASSFP010000434 DASSFP010000000
VERSION     DASSFP010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277986"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1611
                     /locus_tag="VFH91_09790"
     CDS             268..1611
                     /locus_tag="VFH91_09790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6863331.1"
                     /translation="MIRRFIVSVLRLALRSYFQRIEVTGLDHVPKNTPAIFVLNHPNA
                     LVDPVFLLCLAPRRVSFLAKAPLFRMPVIGYFVKALDSLPVYRRQDEGEDVSKNQETF
                     VAARKLLARGGTIGICPEGVSHNEPRLRPIKTGAARISLAALATGEVRSLKIVPAGLY
                     YTAKTRFRSSALLYFGEPIEVERVQLESDGTPPKAAVRELSDKIEIALRAVILDAEHE
                     EALLTISRAERIFSSEVARDEDERLTGELQLQQLFSRAYPVVQREQPERLRKLEIRMI
                     RFEEELKQAGVDPDELSAPKSTIDVFRKIVTRSFLFLITMGPALLGTVIHFPAYRLGG
                     YLATALSRDSEDVISTIKIISALLLFPLTWIVVAVAAYVLTGWVGALAALIVIPFCGY
                     VAIIFFEELDRSLGGLRALTFFLFRRRFFVRLLAERKAIRNEILSLGKESPLAAD"
     gene            complement(1665..1925)
                     /locus_tag="VFH91_09795"
     CDS             complement(1665..1925)
                     /locus_tag="VFH91_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6863332.1"
                     /translation="MTRQIREQLNDLIDAGTLAAGTLLPSERELANTLDVARNVVRGA
                     YEQLMDLGKIRSEGRKGRSVRSGGGTKKKSGKAAKTTKARKK"
     gene            complement(2103..3236)
                     /locus_tag="VFH91_09800"
     CDS             complement(2103..3236)
                     /locus_tag="VFH91_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET6863333.1"
                     /translation="MQTNALTQVRKGTKELGIKVGILPRGPFNSITDVAGVLVGHKTI
                     IKGDDVRTGVTAVLPHSGNIFLEKVPGAVFVGNGFGKLTGSTQVDELGEIETPIVLTS
                     TLSVPRVADGVIDYVLGLSGNEELQSVNPLVAETNDGYLNDIRGRHITSQDVAAAIKD
                     AKTGIIEEGSVGAGTGTVAFGFKGGIGTASRKLPANLGGYTVGILVQSNFGGILTING
                     APVGQELNRYYLKDELKQAGTSSAKETNPDGSIIIVIATDAPVDARNLKRMAGRSMLG
                     LARTGAAASNGSGDYAIAFSSSSENRIRYSPTGDRVQIVKSLSNNAMSPLFVAVIEAT
                     EEAIYNSLLRATSVSGRGHTVEALPLDRTVEILRKHGLITVGP"
     gene            complement(3535..>4234)
                     /locus_tag="VFH91_09805"
     CDS             complement(3535..>4234)
                     /locus_tag="VFH91_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863334.1"
                     /translation="GVTAEMMLLFTFVILGTSLIWSGFTGVTVAAVIFTIFAMLIRVP
                     VYLLSLLGSKVDRRGRLLIAWFGPSGLSSLLLVLLPVFAGLPGSERLFSVTSLIVLLS
                     VVIHGGSPMLLAGKSEEQKAAGTPEVPPAPVEPVEPAKPERALPVITESEPSTQEADG
                     APIGAQSITLQELDELQKSGEPVIILDVRTERSIEDSDLQVRGAVRLPPEHVAIDARD
                     LGLPKNAWLIAHCA"
BASE COUNT          983 a         1091 c         1076 g         1084 t
ORIGIN      
        1 ttttgagcta aggttgcgaa tgcagtggag gcaaccagca gtaaggacaa tgcaagaaac
       61 acaaagcgct tcatatctat tcctcaagtc gtggatttgt gaaccggttg ggaaagcgcg
      121 gatattattg cactaaattt cggtccgcgc atcttacata ccgcacaaat tattcctcag
      181 gatcctactg gctcagaagg ctgacggctt tccattgact tttatccgac tgacggcgac
      241 attggcttac aaatattgcg ctgacctatg attcgccgct tcatagtttc ggttttaaga
      301 ctcgcgctga gaagctactt ccagcgaatt gaggtcacag gtttagacca cgttccaaag
      361 aatactccgg ccattttcgt tctgaatcat cccaatgcgc ttgtcgatcc ggtttttcta
      421 ctgtgtcttg ctcctcgacg cgtctcgttt ctagcgaagg cgcctctgtt tcgtatgccg
      481 gtaatcggat atttcgtcaa agcgctcgat tctttgcccg tgtatcgccg gcaagatgaa
      541 ggcgaagatg tgtcgaaaaa tcaggagaca ttcgtcgctg ctcgcaagct attggcacgc
      601 ggcggaacga tcggaatttg tccggaaggc gtttctcaca acgaaccacg tctccggccc
      661 attaaaacgg gagcagctcg aatttcactc gccgcattgg ccactggcga agttcgctca
      721 ttgaagattg ttccggctgg tctttattac accgccaaga cccgatttcg aagttccgcc
      781 ttgctttact tcggcgagcc aattgaggtc gaacgagtac aacttgaaag tgatggcacg
      841 cccccgaagg ctgccgtgcg agaactctca gacaaaattg aaatcgcgtt acgcgcagtc
      901 attctggacg ccgagcatga agaggcgctg ctgacaatct cgagagcaga aagaattttt
      961 tcttctgaag tggctcgcga cgaagatgaa aggctgactg gagagcttca attgcagcag
     1021 ctctttagca gggcttatcc agtcgtacag agggaacagc ctgaaaggtt gcgcaagctg
     1081 gagatcagga tgattcgttt cgaggaggag ctaaaacaag ctggcgtgga tcccgacgag
     1141 ctgtcagcgc caaaatcaac aatcgatgta tttcgaaaaa ttgtgacgag atcgttcctg
     1201 tttttgatca cgatgggacc agcgctcctc ggaacagtga tccacttccc ggcctacagg
     1261 ttgggcggat accttgccac cgcactatcc cgagattccg aggacgtgat ctctactatt
     1321 aagataatct ccgcgctgtt gcttttccct cttacctgga tagtagtcgc ggtcgctgct
     1381 tatgttctga ccggatgggt gggcgctttg gccgccctga ttgttattcc attttgcggt
     1441 tatgtcgcga tcatcttctt tgaggaatta gatcgttcgc tcggtggact gcgtgcgtta
     1501 accttctttc tatttagaag gaggttcttt gtgagacttc tggccgaacg taaagcgata
     1561 agaaatgaaa ttctctcgct aggcaaagaa agtccattag ctgctgacta gcactgaggc
     1621 ccgtgaaaaa ggggaacctg gttggggggc gatcgagatt agtgctactt cttccgagcc
     1681 ttcgttgtct ttgcagcctt accggacttt ttcttagtgc caccacctga acgcacgcta
     1741 cggcccttcc gaccttcgct gcgaatcttt cccaggtcca tcaattgctc ataggctcct
     1801 cgaaccacat tgcgtgcgac atcaagagtg ttagccagct cgcgttcgct tggaagaagt
     1861 gttccagccg cgagtgtgcc ggcgtctata agatcattca actgttctcg aatttgcctc
     1921 gtcatggact caggcttttt tcgattaaca cgaacattta gtttcacctt attggctccc
     1981 ttcaagagtg agttggtccg gggggtacaa acgaattcta tgagcactac atctgagtgt
     2041 caaacatcat gcggtctata agatgaaaag ttagaccacc gtgaagttgt cgtgatagag
     2101 atttaagggc cgacggtaat caaaccatgt ttgcgcaaaa tttccacggt gcggtccaac
     2161 ggtaaggctt cgactgtatg cccgcgtcct gagacagacg tggcccgtaa tagtgaattg
     2221 tagatcgctt cctccgtggc ttcgataacg gcgacgaaga gtggggacat tgcattgttg
     2281 ctcagagatt tgacgatttg cacacggtcc ccagtcggac tgtagcgaat gcgattctca
     2341 ctggagctgg aaaatgcgat cgcgtagtcg ccacttccgt tacttgcggc agcgcctgtc
     2401 cgggcaagtc ccaacataga acgtccagcc attcgtttta agtttcgagc gtcaacgggc
     2461 gcatccgtgg cgattacaat gattattgag ccatccgggt tggtttcttt tgctgaactg
     2521 gtcccggcct gtttaagttc atcctttaaa tagtaacgat tcagttcctg acctaccggt
     2581 gcaccgttga tcgtcaaaat cccaccgaag tttgattgca ccagaatccc gaccgtgtag
     2641 ccacccaggt ttgcgggaag tttcctggaa gccgtgccga tacccccctt gaatccaaat
     2701 gccacagtgc ccgtaccggc tcctacagat ccttcttcaa taattccagt tttcgcatct
     2761 tttatggccg cggccacatc ctgcgaagtg atgtgtcgtc ctcgtatgtc gttcaagtat
     2821 ccgtcgtttg tttcagcgac aaggggattt accgattgaa gctcctcgtt accgctgagt
     2881 cctagtacgt aatcaatgac gccgtcagcc acacgcggca cgcttagcgt tgacgtaaga
     2941 acaatcggag tctcaatctc acctaactcg tctacctggg tcgatcccgt tagcttgcca
     3001 aagccgttgc cgacaaaaac tgcacccgga actttctcga ggaagatatt tcccgagtga
     3061 ggaaggactg cggtgactcc ggttcgtacg tcgtcacctt tgataatcgt cttgtgtcca
     3121 accaatactc cggcgacgtc agtaatggaa ttgaaaggcc ctctcgggag aatgccgact
     3181 ttgatgccca gttccttcgt tcccttacgg acttgagtta aggcgtttgt ttgcatcggt
     3241 ggtccattgt tggatctttg tgggtgatag taagtaatgc agagaaatga aactaatatg
     3301 caaattggag ggagtttgag gttcattttc agaagttagg actggagcag ctgatttgac
     3361 aaaccggcgc cgggacgccg cactccaaag ttaagctcca accttggtgg ttcttggttc
     3421 aaccggaagg tttgcctgtt gccaggcgtt ccatccaccg atgagcgctc tcgctttggt
     3481 ccaacctgct tgtcttagtt cctgtgccac acgggcactg gtagcttcgt ttggtcaagc
     3541 gcagtgagcg atgagccagg cattcttggg aagccccaga tctcgcgcgt cgatggccac
     3601 atgctcgggc ggtaatctca cggctccacg aacttgtaag tcactatcct caatgcttcg
     3661 ttccgttctg acgtcaagga ttatcactgg ctcgcctgat ttttgaagtt cgtctaactc
     3721 ctggagcgtg atcgattgag ctccaattgg agcgccatca gcttcctgag ttgacggctc
     3781 agactccgtg ataactggta acgcccgctc aggtttcgct ggttcaacgg gctcgaccgg
     3841 agctggagga acctccgggg tgcctgcagc cttttgctct tcactcttcc cggccagaag
     3901 cattggcgaa ccaccatgta tgacgactga cagcaacacg attaaggaag ttacggaaaa
     3961 aagtcgctcg ctacccggaa ggccagcaaa tacaggcagc aagactaaga gcagggaact
     4021 cagtccgctc ggaccaaacc acgcgatcaa cagtcgtcca cgtcgatcga ctttcgagcc
     4081 caacagggac aagagataga cagggactcg gatgagcatc gcaaatattg tgaagataac
     4141 agcggcaacg gtgacaccag tgaacccgct ccagatcaaa gatgtgccta atatcacaaa
     4201 cgtaaagagc aacatcatct ccgctgtcac tccg
//
LOCUS       DASSFP010000435         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_278204, whole genome shotgun sequence.
ACCESSION   DASSFP010000435 DASSFP010000000
VERSION     DASSFP010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278204"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VFH91_09810"
     CDS             <1..841
                     /locus_tag="VFH91_09810"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863335.1"
                     /translation="DGKPGPATQVALKKFQADRHLPVPGKLDSITLSALGVSVANTKG
                     TEPSSNIILADAVNSLVHTVDADQRPGFLAATNAGLFRSFDPAKGWQKLSYDIDVDSR
                     TTCISNNMKAPETIWVGTASSGILVSHDSGKTWSKVEGISTDFPINTIVQDPQRPSYV
                     YVGTKQSLFMSHDGGATWHRRGGNLPFGDFTSILVNPRNGDEIFAGNAYQTSDVGGGV
                     YRTTNGGVTWARIDSKERRLPSQRIWVLAFDANDQNILFVGSHSAGIYVVPRTSENIS
                     SSQ"
     gene            1235..3076
                     /locus_tag="VFH91_09815"
     CDS             1235..3076
                     /locus_tag="VFH91_09815"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET6863336.1"
                     /translation="MSSEQPKLNNSEADNNSTGEQQSSPQTTAAAAQSSPAPAAHDAD
                     DSDESRASGDVDFGQLLDQFEQEQATLEEGEVVRGIVVNLSERGVVIDFGYKSEGIVN
                     PAEFTENGVLTVKPGDEVEVLVKSMETADGLPVLSRADAVRMKAWDDLEKAYRDGSSV
                     KGRVIDRIKGGLRVDIDGIAAFLPGSQVDVRPVRNLDTLRNQIIEAKVIKLNRKRSNV
                     VLSRKAVIEQENTGRKDQTLQQIEEDIVIEGQIKNLTDYGAFVDLGGVDGLLHVTDMS
                     WGRLQNPNELFKVGDTIQVKVLKFDRDRERVSLGYKQLLPDPWSSVEERFPVGTRVNG
                     RVASVADYGAFVELENGVEGLVHVSEMSWSKRVKHPSKVVNPGDLVEVEVLSVDPKAR
                     RISLGMKQVQENPWQTLHERYQVGTRVHGRVRNLTDFGAFIEIEDGVDGLVHVSDISW
                     SRRIKHPSEVLKKGQEIDAVITSIDSENRRLSLSIKDLEPNAWEKFTNEHKPGDVVRG
                     KVARFANFGAFIELDDNLEGLCHISELSEERVAKPEDVVQLGQEMDFKILRIDPDTKK
                     IGLSARAVGKDEPIVDTKIYSSEAGGGMASLGELADFGLNKSESNEN"
     gene            3333..>3484
                     /locus_tag="VFH91_09820"
     CDS             3333..>3484
                     /locus_tag="VFH91_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6863337.1"
                     /translation="MTKAELVEDVAREAELTKKDAERLVEIVFESIIETLNQGEKIEL
                     RGFGSFR"
BASE COUNT          893 a          841 c          943 g          807 t
ORIGIN      
        1 tgacggcaag ccaggccctg ctactcaggt tgctctgaaa aagtttcagg cggatcggca
       61 cttaccggta cccggtaagt tggattccat cactctctcc gccctgggag tttctgtggc
      121 gaatacaaag ggcactgagc cctcttccaa tataattctg gctgatgccg tcaactcatt
      181 ggtccacacg gttgatgcgg atcaaagacc tggcttcctg gcggcaacaa acgccggcct
      241 cttccggagt ttcgatcctg cgaaggggtg gcagaagctt tcttatgaca tcgatgtcga
      301 ttcgcgaaca acttgtatct ccaacaacat gaaggcgcct gaaacgatct gggtcggaac
      361 cgcgtcgtcc ggtatcctgg tttctcatga ctctggaaaa acgtggagca aagttgaagg
      421 catctcgact gactttccaa tcaatactat cgtgcaggat ccacagcgac cctcttacgt
      481 ctatgtcgga accaagcagt cgttgttcat gtcgcacgac ggtggcgcga cctggcaccg
      541 acgtggcgga aatcttccct tcggcgattt cactagtatc cttgtcaatc ctcgcaatgg
      601 cgatgagatt tttgcgggaa atgcttatca aaccagtgac gttggtggtg gtgtttatcg
      661 aacaacgaat ggcggggtga cgtgggctcg aatcgactcg aaggagcgtc gcttgccaag
      721 ccaacgaatc tgggtgctcg ctttcgatgc aaacgatcag aatatacttt tcgtcggctc
      781 gcactcggca gggatttacg ttgtgccacg aacctcggag aacatctcct ccagtcaata
      841 aggtttgcac aggaaccccg ctcggaataa gggcggcgtt cgtggaattg tttgcctctg
      901 taggacttgt gtgtatctcc gcgccttgac atgcccttgt tcaggcgtgt acagtgccgc
      961 cctacccgtt aagaataagg cggcgggtgt ttgtcggacc aagcaggtga ctacggcagg
     1021 gcggtaatcg aaacaaatta gctcgacaat tccaaatcca aacggctcgc atcctcgcca
     1081 gctccaaatc ccgacaacgc caaggtcaat aacacacacg ccgcttagaa aaacctgcgg
     1141 cagtcatcga gacctgcgat gattgttccg ttcaggtgag gcgcgatatg atcgtggaac
     1201 gcgctgaaag ttatcgggaa ctacaggagt atgcatgtct agtgaacaac ccaagctgaa
     1261 caacagcgag gccgacaata actcaaccgg tgaacaacag agttcgccgc aaacaaccgc
     1321 agccgccgca cagtcttctc cagcacccgc cgctcatgat gccgacgatt ctgatgagtc
     1381 tcgtgcaagt ggagatgttg acttcggtca actattggac cagttcgaac aggaacaggc
     1441 cacactcgaa gaaggcgaag tagttcgtgg tatcgtcgtc aacctgagcg aacgtggagt
     1501 cgtaatcgac ttcggctata agtccgaagg tatcgttaac cccgcggagt ttacagagaa
     1561 tggcgtactc acagtaaagc caggcgacga agttgaagtg cttgtgaaga gcatggaaac
     1621 tgccgatggt ctgccggtgc tttcacgagc tgatgcggtg cgcatgaaag cgtgggacga
     1681 tctcgaaaaa gcatatcgag atggttcgag cgtcaaaggc cgcgttatcg atcggattaa
     1741 aggtggtctc cgcgttgaca ttgacggcat agctgcgttc ctcccggggt ctcaagtgga
     1801 cgtccgccct gtacgcaatc tcgacacgct gcgaaaccaa atcatcgaag ccaaagtgat
     1861 caagctcaat cgcaagcgat caaacgttgt gttgtcgcgc aaggcggtta tcgagcagga
     1921 gaacaccgga cgcaaggacc agacgctgca acagatcgaa gaagacatcg tcatcgaagg
     1981 tcagatcaag aatcttactg actacggtgc cttcgtggac ctgggcggag tcgacgggtt
     2041 gcttcacgta actgatatgt cgtggggccg attgcagaat cctaacgaat tgttcaaggt
     2101 tggcgatacc atccaggtga aggtgctcaa attcgatcgc gatcgtgaac gtgtttccct
     2161 tggttataaa caactcctgc cggatccatg gtccagcgtt gaagaacgct tcccagttgg
     2221 caccagagtc aacggccggg tcgcaagtgt cgcggactac ggagctttcg tcgagctcga
     2281 aaatggggtc gaagggctgg tccacgtatc agaaatgagt tggtcaaaac gggttaagca
     2341 tcccagcaag gtcgtaaacc ccggcgatct ggttgaggtg gaagtactga gtgttgatcc
     2401 aaaggctcgc cgcattagcc tcggaatgaa gcaagtacag gaaaatccgt ggcaaacctt
     2461 gcatgaacga tatcaagttg gtactcgagt tcatggaaga gtcagaaatc taaccgattt
     2521 cggtgctttt attgaaatcg aagacggtgt tgacggactg gtccatgttt ccgatatttc
     2581 ctggtcgcgc agaatcaagc atcccagtga ggttttgaag aaagggcagg agattgacgc
     2641 tgtaatcaca agcatcgata gcgagaatcg tcgtctttca ctctcgatta aggaccttga
     2701 gccgaacgcc tgggagaagt tcacgaacga acacaaaccg ggcgatgtcg tgcgtggcaa
     2761 agtagctcga ttcgccaact ttggtgcttt tatagaactg gatgacaacc tggaaggctt
     2821 gtgtcacatt tccgaacttt ccgaagaacg ggtggcgaaa cccgaagacg tggtccagtt
     2881 gggccaggaa atggacttca agattttgcg gatcgatcca gataccaaga agataggctt
     2941 atcagcgcga gccgttggta aagatgagcc aatcgtagat actaagatct actcgtcgga
     3001 ggccggcggc ggaatggctt ctcttggtga actggccgat tttggtctta ataaatcaga
     3061 atctaacgaa aactgatact cgtctccagc caactcaagg tggccctgct ttcagctatg
     3121 tcctcaaggt gtggagcggg gctttttctt tttactttga aggcttttaa ttgcattcaa
     3181 agtcgctact gagtgagtct tgcccgacca gattgtttct gatagtgtac ggtgagttaa
     3241 aaaaacctcg cctttatcaa agttaatttg tatttctatt tgcggccctg aacatattct
     3301 caaattggac cattaaacta acgaggtgat gcatgacgaa agcagaactc gtcgaagacg
     3361 tcgctagaga agcagaactg acaaagaagg atgctgagcg tttagtagaa atcgtctttg
     3421 aaagcatcat tgagactctt aatcaaggag agaagataga gctcagaggc ttcggcagtt
     3481 ttcg
//
LOCUS       DASSFP010000436        11142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2220820, whole genome shotgun sequence.
ACCESSION   DASSFP010000436 DASSFP010000000
VERSION     DASSFP010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220820"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFH91_09825"
     CDS             <1..271
                     /locus_tag="VFH91_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6863338.1"
                     /translation="KAGMIGLTKSLAKEVASRKVTVNALALGMVATDMAGKLDQSYQD
                     KIKEQIPLGRFAEPDEVARIAAFLLSDDARYITGQVIQVDGGLAM"
     gene            complement(279..1121)
                     /locus_tag="VFH91_09830"
     CDS             complement(279..1121)
                     /locus_tag="VFH91_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863339.1"
                     /translation="MTTLRLLVLAVSIVTVLVAISNAQAVGQTPTTPSATTVAPGSAD
                     EKSQQIIKHAVEVLGGQAYLNVQTVTGKGFYTAFNDGVTQLPSKFLDYIEYPDRERTE
                     FVSLGIKTIQSNSGNSGWVFDGAVKTIKDQSQTQVDEFKRSMRTSVENLLRGWWQKDG
                     AKVVYVGRREAGLAKRNDAIRLTYPDGYWIEYEFGARDGLPAKIIYKRARKNMDTGDM
                     DETSEEEQLLKFISVDGVTAPWVIDHFSNSKQTSRINYESVQYNQKFADSLFAKPDNV
                     KAIK"
     gene            complement(1244..3535)
                     /locus_tag="VFH91_09835"
     CDS             complement(1244..3535)
                     /locus_tag="VFH91_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6863340.1"
                     /translation="MADLGQYKDKFGASGQRILEHALNESRRRDQNFVSVEHILQALT
                     IEEPDMFNAAMRELSLDPVVVKMAIEKRLSSSRQQHVGKGFRIGPDTTDLFKRAMERA
                     RTQGRKTIESTDIFEALSNQDDSLFLDVLRGMGANPDAVLENVRAEVKRRELEEEQNR
                     KKFELPPYLKHFGVSLNRMARADKIPPTIGREKEIQQIIEILCHRERANSPMLVGEPG
                     VGKTAVIEGLARLIELEPEKVPGRLRNSHIVQLQMGGIVAGTMLRGMFEERIKGIIDE
                     VKERDNLILFIDEAHTIIGAGAALGTSSDAANMFKSALARGEMRIIGATTLSEYKEYI
                     ADDEALARRFRMVKVVEPTINETREILLGLRPRLEKNYSVKISDDAINTALEMAPKYI
                     RNLHLPDKAIGWLDTAAVKVEINEPQTLIVKPEHVIEVISQESRIPRDMIFRDTSDRF
                     AMTEAMLGRRVIGQLAAIKAVAQRLRLNKGPLKESHYKPDGVLLFLGPTGVGKTELAK
                     AVAEFMFGDEEKMVRIDMSEYSDGTVGIEKLIGMPRGIVGSERGGILTEQLRENPYTV
                     LLLDEVEKASPYLMNLFLQGFDEGWLTDGRGKKVYLSDAIIIMTSNLGSDSFKKFEKP
                     LGFGMKTASDFRGVKNEVMKAAEQRFSPEFRNRIDEIVVFSPLTMDEVRQIAKLYLEK
                     LRRQMERQGKLVEVTEAAVNQLTESGFNTAYGARFLKRHIDEKVKLPITGMWKTSSSF
                     IVDVENGEVTVNPSASVSKPELN"
     gene            complement(3740..5368)
                     /gene="groL"
                     /locus_tag="VFH91_09840"
     CDS             complement(3740..5368)
                     /gene="groL"
                     /locus_tag="VFH91_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6863341.1"
                     /translation="MPAKELKFREEARNAMLQGVDILANAVRVTLGPRGRNVVLGKKF
                     GSPVITKDGVTVAKEIELKNKYENMGAQMVREVAVKTNDKAGDGTTTATVLAQAIFRE
                     GVKNVAAGANPMSLQRGIQLATDQVVAELERMSKKVRGKDELTAVATVSANNDAHIGK
                     LISEAMEQVGKDGVVTVEESKTMLTELDIVEGMQFDRGYLSPYFVTNPERMEAILEEP
                     LILLHEKKIAAMRDLLPILEQTVGAGKPLLIIAEDVEGDALATLVVNRLRGTIKVCAV
                     KAPAFGDRRKAVLEDLAVLTGGRVITEDLGIKLENVTIDDLGSAKRVIIDKENTTVVE
                     GGGDRKAIQGRVATIRKQIEETTSDYDREKLQERLAKLAGGVAVVKVGAPTETAMKEI
                     KMRVEDALNATKAAAQEGIVVGGGVALIRASKVLDNFSADDPDVLTGVKILRRALQEP
                     LRRIAENAGIEGAVVIGEVEQSKGNRGFNAVTGKFEDLAAAGIVDPTKVVRTALQNAA
                     SIAGLLLTTDAAITDMPEPKKAATPMPGGDDYDY"
     gene            complement(5400..6053)
                     /gene="grpE"
                     /locus_tag="VFH91_09845"
     CDS             complement(5400..6053)
                     /gene="grpE"
                     /locus_tag="VFH91_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6863342.1"
                     /translation="MNNKERETLRHNSDDANELRVTDRRRVYLDRDENEPIVEEEQPN
                     LKPKYVEELEIRAQAAERQVQEVQQRFDQLRQQLQRETDETRQRLNKAADERAAGEKA
                     RFIGRLLPVMDDLTRAIGAADEGASRDSILEGIKRIAISFQNALANAGVESINAIGED
                     FNPELHEAVETGEASPEMDGKVIDEYSRGFKLGDRLLRPARVKVGRSSGQARIASEQ"
     gene            complement(6080..6526)
                     /locus_tag="VFH91_09850"
     CDS             complement(6080..6526)
                     /locus_tag="VFH91_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6863343.1"
                     /translation="MSQSWNPLQDLIVLQDRMNRLFEDATKRRAHGEPDTSDEFERAD
                     WVPASDIYETQESYSIAIDLPGVARSALEIEVDDNRLIVKGTREIESTKSRRNERPSG
                     KFIKTFSVPPSVDQNLIGAEYKDGVLSVRLPKRQEQKPQKVEIKIS"
     gene            complement(6542..9136)
                     /gene="clpB"
                     /locus_tag="VFH91_09855"
     CDS             complement(6542..9136)
                     /gene="clpB"
                     /locus_tag="VFH91_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET6863344.1"
                     /translation="MRLDKFTLRGQEAIQAAIELAERNQNQQVEPEHLLAAMLDQPEG
                     IVRPVLGKLGANVAVILNDIQAAISRYPRVQGGQQYFSPRLSQIFTASQKQADQMQDE
                     FVSTEHLLLAIVEEKDGESGKILRQHGVTRDDLFKVIEQMRGGSRITDQNAEQNYQAL
                     SKYARDLTEQARKGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVSGDVPETLRNKRLVALDLGSMLAGAKYRGEFEDRLKAVLKEIENTQGQIILFI
                     DELHTLVGAGAAEGAIDASNMLKPALARGELRCVGATTLNEYKKYIEKDAALERRFQQ
                     VYVGEPSVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSNRYITDRFLPDKAIDL
                     IDEAASKLRIEIDSLPQDIDQLEREIIQLEIERQALQREEDEKSKARLHEIEQRIADL
                     KEKSSGMKAKWQSEKEAIEHMRTAKAELEKLRLALDQARNAGDLARASEIQYGQIPEL
                     ELKLKAEQEKLEAFQRDGVMLKEEVDEEDVAEVVAKWTGIPVSKMLEGELQKLVTMEE
                     RLGARVIGQEEALMAVSDAVRRARAGLQDPNRPIGSFIFLGPTGVGKTETARALAEFL
                     FDDERAMIRLDMSEYMEKHAVARMIGAPPGYVGFEEGGQLTEAIRRRPYSVVLFDEIE
                     KAHPDVFNVLLQILDDGRLTDSKGRTVDFKNTVLIMTSNLGSREIQAAEGDEKQVREA
                     VLQELRLNFKPEFLNRVDDVVIFHQLSQQQIGKIIDVQLERLRAMLAERNIKLVLDDS
                     ARDLLMREGYDPSYGARPLKRAIQSLIQNPLAVKLLQGEIGPGQTIKLSANGDTMEFK
                     TDAAAAAA"
     gene            complement(9231..9743)
                     /locus_tag="VFH91_09860"
     CDS             complement(9231..9743)
                     /locus_tag="VFH91_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6863345.1"
                     /translation="MSIVRYDPFRDLRTLQEEVNRLFSTNLTRTFGDEGIGRGAWAPS
                     VDIYENKDQIVLEAELPGMKREDFDLSIENNVITLRGERRFEKTDDTDNYHRVERSYG
                     QFTRSFTLPQSVTGEGATAEYNSGVLRVTLPKREEAKSRRIEVSGTDASPKTIDTKAT
                     DKAKEVAGGR"
     gene            complement(10282..>11142)
                     /locus_tag="VFH91_09865"
     CDS             complement(10282..>11142)
                     /locus_tag="VFH91_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6863346.1"
                     /translation="AAKEPIEVCPRDNLNARLVFFNTLYTDSPAKTIHVVREAVREAD
                     NVSEKITPIVRIRNLGDNGVDYEVKYWLIDYAKYNDTDALVRQRIWYAFRRAGLNFAY
                     PTRSLIVERKGGQGLTERDGGAIVERLSAVDIFAPLSVDETGMLAQAAVRHVFAPGET
                     VIRAGDPGSSMFVVHNGRVQVQVNDNGRPRTVATLNEGDFFGEMALFTGEPRTANVVA
                     IEETEVLEIGHAAMKRVFDTNPDLVESLSFIMSERRQGLATHIDPAAARKAESEGILS
                     AIKRFFGLSQ"
BASE COUNT         2468 a         3015 c         2616 g         3043 t
ORIGIN      
        1 aaaagccggt atgattggtc tgacgaagtc gttggcaaag gaagtcgcaa gcagaaaagt
       61 gacagttaac gcgctcgcgc ttggtatggt cgctacggat atggcgggaa agttggatca
      121 aagttatcag gacaagatta aagagcagat accgctgggc cgatttgctg agccagatga
      181 ggtggcaaga attgctgcat tcctgctttc agacgatgct cgatacatca caggccaggt
      241 aattcaggtt gatggtggac tggcaatgta ggtcggcgct acttaatagc cttcacgttg
      301 tcgggctttg cgaaaaggct atccgcaaac ttttgattgt attgaactga ctcgtagttt
      361 atccgtgatg tctgtttgga gttgctaaag tggtcaatca cccatggagc tgtgactcca
      421 tcaactgaga taaacttcaa cagttgctct tcttccgagg tctcatccat atcgccggta
      481 tccatgtttt ttctcgcccg tttgtagatg atttttgcgg gtagaccatc tcgcgcccca
      541 aactcatact cgatccaata accgtcaggg taagtcagtc ttatcgcatc gtttcttttt
      601 gcaagccccg cctcacgccg tcccacgtag acaactttag cgccgtcttt ttgccaccag
      661 cctcgcaaga gattctcgac gcttgttcgc atagaccgct taaactcatc tacctgtgtt
      721 tgactctggt ccttgattgt cttgacggcc ccgtcaaaca cccaacctga attgccactg
      781 ttcgattgaa tggtctttat tccgagactc acaaactctg tgcgctcgcg atccggatac
      841 tcgatatagt cgagaaactt tgagggtagc tgtgtgacgc cgtcgttgaa cgcggtgtag
      901 aagcctttgc cagtcacagt ttgcacgttc aggtaggcct ggccacccag tacctcgacg
      961 gcatgcttga ttatctgctg agacttttcg tcggcagaac ccggtgcaac tgtcgttgcc
     1021 gaaggagtgg tcggcgtctg acctacggct tgagcgttcg aaattgcaac aagaacagtg
     1081 acgatcgaga ccgcaaggac tagcaggcgc agtgttgtca tatcaaaaag agtgaagcgt
     1141 gcggattgag cagttgctcg tggttttgaa tctcagttac gaaaagaggg ctccagttga
     1201 atatcatcac tgaaaccctc ttgtccgaca ataggctaaa gccttagttc aactctggtt
     1261 tcgacacaga ggcgcttggg ttaacggtta cctctccgtt ttcgacgtct actatgaaac
     1321 tcgacgacgt tttccacatt ccggtgatcg ggagttttac tttttcgtcg atgtgacgtt
     1381 ttagaaagcg cgcaccgtag gcagtgttaa agccactctc ggtgagttga ttaaccgccg
     1441 cttccgtaac ctcaacaagt ttgccttgtc gttccatttg ccggcgcaac ttttcaagat
     1501 acagcttggc gatttgtcgt acctcatcca ttgttagtgg tgagaagacg acgatctcgt
     1561 cgatgcggtt acgaaactcc ggtgagaatc tctgctcggc tgctttcatc acctcatttt
     1621 taacgcctcg gaaatcagat gctgtcttca ttccgaatcc gagtggcttc tcaaatttct
     1681 tgaagctgtc agatccgaga tttgacgtca tgatgattat cgcgtccgac agataaacct
     1741 tcttgccgcg tccgtccgta agccatccct catcgaatcc ctgcagaaac aggttcatca
     1801 agtatggcga agctttttcc acctcgtcca aaagcaggac tgtataagga ttctcgcgca
     1861 actgttccgt gagaatgccg ccccgctccg aaccgacaat accccgcggc atgccgatta
     1921 acttttcgat tccgacggtg ccatcgctgt actctgacat gtcgatacgc accatcttct
     1981 cttcatcacc gaacatgaat tcggcgacag cttttgctaa ctccgtttta ccgacaccag
     2041 ttggaccaag gaatagcagc acgccatcgg gtttgtaatg agactccttc aagggacctt
     2101 tatttaacct gagacgttgg gccactgcct tgatagcggc caactgtccg ataacacgcc
     2161 ttccaagcat tgcttcagtc atcgcaaaac gatcgcttgt gtcacggaag atcatgtcgc
     2221 gcggaatacg agattcctgg gagatgacct cgatgacgtg ctccggtttg acaatcagtg
     2281 tctgcggttc gtttatctcg acttttactg cggcagtatc gagccagcca atcgccttat
     2341 cgggcaaatg aagattgcga atgtacttcg gcgccatttc caacgccgta tttattgcat
     2401 cgtccgaaat tttgaccgag taattcttct ccagccttgg ccttaaaccc agcagaattt
     2461 cccgggtctc attaatagtt ggctcaacga ctttaaccat tctaaagcgg cgggccagtg
     2521 cctcatcgtc cgctatgtac tctttatatt cagacagagt cgtcgcgccg ataattcgca
     2581 tttcgccgcg agccagagcc gatttgaaca tgtttgccgc gtcggaagac gttcccagag
     2641 cggcaccggc gccgataatc gtatgtgctt catcgatgaa gagaatcagg ttgtcccgtt
     2701 ccttcacttc gtcaattatt cccttgattc tttcctcgaa cattccccgc aacattgttc
     2761 cagctacaat tccgcccatt tgcaattgaa caatatggga attgcgcaga cgtcccggaa
     2821 ctttttcggg ttcgagttca atcaggcgag cgaggccttc gatgaccgca gttttgccaa
     2881 cacctggttc accgacgagc atcggcgaat tcgcgcgttc tcgatgacaa aggatctcaa
     2941 tgatttgttg gatctctttt tcccgcccga ttgttggtgg aatcttatct gcacgggcca
     3001 tccggtttaa ggacacgcca aagtgcttca aataaggagg aagctcaaat tttttgcgat
     3061 tttgctcttc ttctaattcg cgtctcttca cttcggcgcg aacgttttcc agaacagcat
     3121 ctggatttgc gcccatccct cgtaaaacat cgaggaagag actgtcatct tgattgctca
     3181 gagcttcaaa aatgtcagtt gattcgattg ttttgcggcc ctgtgtacgg gcacgttcca
     3241 tcgcccgttt gaataggtct gtggtgtccg gaccgattcg gaatcctttg ccaacgtgct
     3301 gttggcggct gctgctcagc cgtttttcaa ttgccatctt gaccactacg ggatcgagcg
     3361 ataactcccg catcgcggca ttaaacatat ctggctcttc tatcgtcaga gcttgcaaaa
     3421 tatgctcgac tgaaacgaaa ttttgatcac gacgacgcga ctcgttcaac gcatgctcga
     3481 gaatgcgctg tccgctggcg ccaaatttat ctttgtattg acctagatct gccataattc
     3541 atatacgacc gtcgaccctt cttgggatcc ataacaccga cggtaaacac tcaactttca
     3601 aacagctcaa ccctacaatc taaccgccac gttagatgtc gtcaggataa gggccgttgc
     3661 gtttcaatgc aataactaat accggcagga gactgcaagc aaatctcctg ccgttatgtt
     3721 gtaaaaaacc cgttaaatat taataatcgt aatcgtctcc ccctggcatc ggtgtagccg
     3781 ccttcttagg ctcgggcata tccgtaattg ccgcgtccgt agtcagcaat aagccggcaa
     3841 tggaagccgc attttgcagg gcggttcgga ccactttggt gggatcgaca atgccggcgg
     3901 cagccaaatc ttcaaacttg cccgtcaccg cgttgaatcc cctgttacct ttcgactgtt
     3961 caacttcacc aatgactact gctccttcga ttccagcatt ttcagcaatt cgacggagcg
     4021 gttcctgtag agctcttctt aagatcttca cgccagtgag cacgtccgga tcgtctgctg
     4081 aaaagttgtc caacactttt gaagcacgaa ttaaagccac gccacctccg acaacaatgc
     4141 cctcctgagc cgctgccttg gtagcgttca atgcgtcttc cacccgcatc ttgatctctt
     4201 tcatcgcagt ttctgtggga gcgcctacct taacgacggc aacaccgccg gcaagttttg
     4261 caagtcgttc ctgcaatttc tcacgatcat aatcggatgt tgtttcctcg atctgtttcc
     4321 ggatcgtggc gacgcgcccc tggattgctt tgcgatcacc accaccttcg acaacagtcg
     4381 tgttctcttt gtcaattatc actcgcttag cagagccgag atcatcaata gttacgttct
     4441 caagtttgat tccgaggtct tccgttatga ctcgaccacc ggtgagaact gcgagatctt
     4501 caaggaccgc tttccgccga tctccaaaag ccggagcttt caccgcgcac actttgatcg
     4561 tgccgcgcaa acggtttacg acaagtgtgg ccagtgcgtc accttctaca tcttcggcaa
     4621 tgatcaataa cggtttgccg gccccgactg tctgttccag aatcgggaga agatcgcgca
     4681 tcgcagcaat cttcttctcg tgcaggagta tcagaggctc ttcaagaatg gcttccatcc
     4741 gttctggatt cgtgacgaaa tatggagaca aatatcctcg gtcaaactgc attccttcta
     4801 caatatccag ttcggtgagc atcgtttttg attcttctac ggtaacgacc ccatctttgc
     4861 caacttgttc catcgcttcg gagatcagct tgccgatgtg ggcgtcgttg ttcgcggaaa
     4921 cagttgcgac cgcggtcaac tcgtctttcc cgcgtacttt cttcgacatt ctttccaact
     4981 cggcaacaac ctggtcggtg gccagttgaa tgccacgttg cagcgacatc ggatttgctc
     5041 ctgctgcgac gttcttcaca ccttcgcgaa aaattgcctg agccaaaaca gtagctgttg
     5101 tggttccatc gcctgcttta tcgttggttt taaccgcaac ttcgcgcacc atctgtgcgc
     5161 ccatattttc gtacttgttt tttaactcga tctccttggc aacggtgacg ccatctttgg
     5221 taatcactgg cgaaccaaac tttttaccaa gtacaacgtt gcgacctctt ggtcctaacg
     5281 ttactcgaac cgcattagcc aggatgtcga ctccttgtaa cattgcgttc cgagcttctt
     5341 ctctgaattt caattctttt gcaggcactc ttctgttctc cttatcaacg gtcaataaac
     5401 tactgttctg acgcgatcct tgcctggccc gacgaacgtc caacctttac ccgggctggt
     5461 cgcaagagcc ggtcgcccaa cttaaagcct cgcgaatatt cgtcaatgac tttgccgtcc
     5521 atttctggcg aagcctcacc tgtttcaacc gcttcgtgta gttcaggatt gaagtcttca
     5581 ccgattgcat ttatcgactc aactccagcg ttggcgaggg cgttctgaaa gctaattgcg
     5641 attctcttta tgccttccaa aatgctatct cgtgatgcgc cttcatctgc tgctccgatg
     5701 gcacgagtca aatcgtccat cactggaagg agcctcccga tgaatcgagc cttttcgccg
     5761 gcagctcgct cgtctgcagc tttgtttaat cgttgtcttg tttcgtctgt ttcgcgttgc
     5821 agttgctgtc gcagttgatc gaatctctgt tggacttcct ggacctgacg ctccgctgcc
     5881 tgagcacgaa tctcaagttc ttcaacgtat tttggcttca ggtttggctg ttcctcttcg
     5941 acgatgggct cattctcgtc gcgatcgaga taaacccggc gacggtcggt tacacgcagc
     6001 tcattagcat catcgctatt gtgtctcaaa gtctcccgct ccttgttatt catcaacgtc
     6061 ccccttgcgc acaccaactt caggaaattt taatttccac cttctggggc ttctgttctt
     6121 gtcgttttgg aagcctgacc gataaaacac cgtccttata ttctgccccg atcaggttct
     6181 ggtcaaccga agggggaaca ctaaatgttt tgatgaattt ccctgagggc ctttcattgc
     6241 gcctgctctt ggtcgattca atctctcttg tacctttgac aatcagtcga ttgtcgtcaa
     6301 cttcgatttc gagtgcgctt cgtgcaacac ccggcaagtc gattgcaatt gaatatgact
     6361 cttgcgtctc ataaatgtcc gaggcaggaa cccaatcagc gcgctcgaac tcatcagaag
     6421 tgtccggctc accgtgcgct cttctttttg tagcgtcctc gaacagccta ttcatccgat
     6481 cttgcagcac aatcaaatcc tgcaacggat tccagctttg tgacatgttt atctccagga
     6541 tttacgcagc ggcggctgcg gcgtccgtct taaactccat ggtatcgcca ttggccgaca
     6601 gcttgatcgt ttgaccgggc ccgatctcgc cttggagcag ttttaccgcg agcggatttt
     6661 gaatcaacga ttgaatcgcg cgctttagcg gcctggcgcc gtagcttgga tcgtaacctt
     6721 ctctcatcaa taaatctcta gcggagtcat cgagcaccag ttttatattt cgttcagcca
     6781 gcatcgctcg aagtcgctcg agctgtacat cgatgatctt cccaatctgt tgttgtgata
     6841 gttgatggaa aatcacgaca tcatcgactc ggttgagaaa ttccggtttg aagttcagtc
     6901 gcagctcttg cagcactgct tcccgaacct gtttctcgtc gccctcggcg gcttgaatct
     6961 cgcgagaccc aagatttgag gtcataatca gaactgtgtt cttgaagtcg acagttcggc
     7021 ccttcgaatc ggttaatcgt ccatcatcca gaatttgcag caggacattg aatacatcag
     7081 ggtgcgcctt ctcaatctca tcaaacaaga ctaccgaata aggacgacgg cgaattgctt
     7141 cagttagctg tccaccttcc tcaaatccca cgtaacctgg aggcgcgcca atcatacgtg
     7201 cgacagcatg tttttccatg tattccgaca tatccagacg gatcatggcg cgctcgtcgt
     7261 caaataaaaa ttctgctaat gctcgggccg tttcagtttt gccgacacca gttgggccaa
     7321 ggaagataaa ggagccgatc ggccgattag gatcctgcaa acctgcgcga gcgcgtcgta
     7381 cagcatcgga tacagccatc aaagcttctt cctgcccaat aacgcgcgca ccgagacgct
     7441 cttccatggt tacgagtttc tgcaattcac cttcaagcat cttggaaacg ggtatgccgg
     7501 tccacttagc aactacttcc gcgacatcct cttcgtcaac ttcttctttc aacatgacgc
     7561 cgtcgcgttg aaatgcttcg agcttttctt gttcagcctt aagcttaagt tcgagctcgg
     7621 gaatctgacc gtactgaatc tcggaggcgc gagctagatc gccggcgttg cgggcctgat
     7681 cgagggccag acgcaacttc tccaactcag ccttagcggt tcgcatgtgt tcgatcgctt
     7741 ctttttcaga ctgccacttc gccttcattc cggaagactt ttctttgaga tcggcgatcc
     7801 gctgttcaat ttcgtgcagc cgagcctttg acttttcgtc ttcctctcgc tgcaacgcct
     7861 gccgttcgat ttcaagttga atgatctctc gctcaagctg atcgatgtct tgcggcagtg
     7921 agtcgatctc gattcgcagt tttgaagccg cctcgtcaat cagatcgatg gccttgtcag
     7981 gaagaaaacg atccgtgatg tagcgattcg aaagtgtagc tgcggccaca atcgcggaat
     8041 ctttgatgcg gacgccgtga tggacctcgt atctctcttt tagaccacgc aagatcgcaa
     8101 tggtgtcttc tactgacggt tcaccgacat aaacctgttg aaagcggcgc tctaatgcgg
     8161 cgtccttttc gatgtatttt ttgtattcgt tgagcgtcgt cgcgccgaca caacggagtt
     8221 cgcctcgagc cagggcaggc ttcaacatat ttgaagcatc aatggcgcct tccgctgcgc
     8281 ctgctcctac tagtgtgtgc aattcgtcga tgaagagaat gatctggccc tgagtgtttt
     8341 cgatctcttt caaaaccgcc ttaagtcgat cctcaaactc tccacgatat tttgcgccag
     8401 ccagcatcga gccgagatca agcgccacca atcgcttatt gcggagagtt tccgggacgt
     8461 caccagacac tatccgttga gcgagacctt caacaatagc cgtcttacct acgcctggct
     8521 cgccgattaa gaccggatta tttttggtgc gtctcgaaag aacttgaatg gtccgacgaa
     8581 tttcgtcatc gcgacctatt accggatcaa gtttgccctt acgagcctgt tccgtcaggt
     8641 cccgagcata tttggaaagg gcttgataat tctgctccgc gttttgatcc gtaatgcggg
     8701 aaccaccgcg catctgctcg atgaccttga agagatcgtc tcgagtgacg ccatgttgac
     8761 ggagtatctt tcctgattcg ccgtcctttt cttccacgat cgcaaggagg agatgttcag
     8821 tagagacaaa ctcgtcctgc atttgatcgg cctgtttttg tgacgctgta aagatctgac
     8881 tcagacgagg gctgaaatac tgctggccac cttgaactcg cggatatcgc gagatggccg
     8941 cttgaatgtc attaagtatg accgcaacgt tggcgccgag cttaccaagc accggcctta
     9001 cgattccctc gggttggtcc agcatcgcag caagtagatg ctcgggctcg acctgttggt
     9061 tctggtttct ttcggccagt tctattgctg cctggattgc ttcttgtccg cgaagggtaa
     9121 acttatctaa tcgcattacc ttattccgaa agtttagcta tcaaacccaa acgggcccgc
     9181 ttatgcatcc caaactgcag cgagcccgct cctactgcat gcccccttga ttacctgccg
     9241 ccagctacct ctttggcctt gtccgttgct ttcgtgtcga tcgttttggg tgaggcgtcg
     9301 gtcccactca cctcaatccg acgtgacttg gcctcttcgc gtttcggcag cgtcacgcga
     9361 agcacaccac tgttgtactc ggccgttgcg ccttctcctg taacgctctg aggtaaggtg
     9421 aatgaccggg tgaattgtcc ataagaccgt tcaaccctgt gatagttgtc ggtgtcgtcg
     9481 gtcttctcga atcgtctttc gccccgaagg gtaataacgt tgttctcgat tgataaatcg
     9541 aaatcttccc tcttcatgcc gggaagttcg gcttcaagga ctatctgatc cttgttctcg
     9601 taaatgtcga cgctaggcgc ccaggcgcct ctgccaatac cctcgtcacc gaaggtacgc
     9661 gttagattcg ttgagaacag acgattaact tcctcttgta gagtgcgaag gtcccgaaac
     9721 gggtcgtatc gaacaatact cataactgaa acctctcctt atgcaaattc cctatttgtc
     9781 gctcgaagcc tttttcgatt caatataaag cggctgagcg tgaccgcata ataaagattg
     9841 agtgtgttgt tgtcaagtct gaaattagac gacgaattca aaggggaaag aggtcaaatg
     9901 atgaggaaaa aggggtccaa gagttgcgcc ttgataaccc tgagttgtcc tttataagag
     9961 gagtgtgaag gcggatgacg aaaaaattat gtctgatatg ctttcagctt gtcggtcgtc
    10021 gttgactcgt tcccctgaac ttgagggtgc atcatgacga caagctgaag aagcatatcg
    10081 gacattgagg taagagagga acgtgaaggc ggatggctcg aggctggagc ccagaaccgc
    10141 tcaaccgatc cgttagacga gaaaattatg cgagcagtgt ccgatacgct tcagcttgtc
    10201 ggggtcgtcg ttgacccttt cctctgagct tgagtgtgca tcatgacgac aagctgatga
    10261 agcatatcgg acattgagct tttattgaga aaggccaaaa aaccgcttga ttgctgacag
    10321 gataccctcg gactcggctt tcctggcagc cgcaggatca atatgagttg ccagcccttg
    10381 ccgcctttct gacattataa aactgagaga ttcgaccaga tcggggttgg tatcaaacac
    10441 tcgcttcatt gcggcatgcc caatttcgag gacctctgtt tcctcgatcg cgacgacgtt
    10501 cgccgtgcgg ggttcgccgg taaacagggc catctcacca aaaaagtcac cctcgttcag
    10561 ggttgcaact gttcgaggtc gcccattgtc gttgacttga acctgaacgc gaccgttatg
    10621 aactacaaac attgaagacc ccgggtcgcc agcgcggatg acggtctcgc cgggtgcgaa
    10681 aacgtgtctg actgccgctt gtgccagcat ccccgtttca tcgactgaca atggcgcaaa
    10741 aatatcgaca gcagaaagtc gctcaacaat agctccaccg tcgcgttccg tcagaccttg
    10801 tccacctttg cgctcgacga taagacttcg agtcggatag gcgaagttga gcccggcacg
    10861 tcgaaacgca taccagattc tctgtcgcac taaggcatcc gtgtcgttgt attttgcgta
    10921 atctataagc cagtacttca cttcatagtc gacgccatta tcacccaaat tccggatacg
    10981 aacgatcgga gtaattttct ctgaaacgtt atcggcctcc cgaacggcct ctctaactac
    11041 gtgaatagtc ttcgcaggtg agtcggtata caaggtattg aagaagacca accttgcgtt
    11101 gagattgtca cgcgggcaaa cctcgatggg ctctttggca gc
//
LOCUS       DASSFP010000437         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_568362, whole genome shotgun sequence.
ACCESSION   DASSFP010000437 DASSFP010000000
VERSION     DASSFP010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568362"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFH91_09870"
     CDS             <1..608
                     /locus_tag="VFH91_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6863347.1"
                     /translation="CGQTASTGALTGIALDPFGAALPVVTIDLIREGGSEKRSVLSDE
                     NGLFAFPLLPPGKYELHASKANCRPVSLLELQISVTETLKLEILLQLAANYEQAKVSS
                     SLQMVQTETSALGRVVDERGVLSLPLVTRNFTQISGLSPGVVAGVFNSGELGLGGTAD
                     GVWDRFREQWYRDCRWTRTSKFGSGDFESCNSELAPRGKCA"
     gene            complement(897..2357)
                     /locus_tag="VFH91_09875"
     CDS             complement(897..2357)
                     /locus_tag="VFH91_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863348.1"
                     /translation="MPADMATFHRILFAIVTVILGFATALCAEAQSQASGLSLYAGAQ
                     PASDAGSPAARVQPLKPVRISKEEQRGVDWVHLVGQSFFFMSFENAFRCATEEGTRDG
                     FSNPFFRGYLNSVGNLHGWSDGDPFLVNYVGHPMQGAVTGYIWTQNDRAYRDIQFGQN
                     RRYWKGKLRGAAYSYVYSVLFEIGPISEASIGNIQHYYPQVGFVDHVVTPAIGLGWSI
                     AEDALDQYFIRYLERRTSNNWVRLLVRGGLNPARSMANVVALRTPWYRANRPGVLSAE
                     LGDSEFMEALGKRNTTPMEVSPPPGVSPFEFNLTAKVKTYLGNHHAAPCVGGGGTGAV
                     RVASEWQFIVDVSGCKLLGLGDNLSGDSLTYLAGARWTPHPVGRWTTHAEVLIGGSKL
                     TQELIDPSIEKAVKSSASKSTPAYKLYPQYAKHWETNGFTVQAGSGIDVKLNSALSYR
                     VAQLEYSHSWTNDLNGISYQHSLQFSSGLILHVGTW"
     gene            complement(2716..>2896)
                     /locus_tag="VFH91_09880"
     CDS             complement(2716..>2896)
                     /locus_tag="VFH91_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6863349.1"
                     /translation="TVYVGQLDLSVYDSANKQLVWRGVATKTLDPKAKPEKKQKNVTK
                     AVAKLLKNFPPKPKT"
BASE COUNT          688 a          755 c          775 g          678 t
ORIGIN      
        1 tctgcggcca gaccgcctcg accggcgcgc tcacaggaat tgctctcgat cctttcggtg
       61 ccgctcttcc ggtagttact atagacctaa tccgggaagg cggcagcgag aaaaggtccg
      121 tgctttcgga tgagaatgga ctgttcgcct tccccttatt gccgcccggc aaatatgagt
      181 tgcacgcaag caaggccaat tgccggcccg tgagcctctt agaattgcaa atatccgtaa
      241 ccgaaacgct gaaactcgaa atccttcttc aactcgcagc caattatgag caggccaaag
      301 tgagttctag cctgcaaatg gttcaaactg aaacgtctgc tctcggccga gtggttgacg
      361 aacgaggtgt tttgagtctg ccactggtca ccaggaactt tacccagatc agtggactgt
      421 ctcccggcgt agttgctggg gtcttcaact ccggagaact tggacttggt ggtactgctg
      481 acggcgtttg ggaccgcttt cgggaacagt ggtacaggga ttgccgatgg acccggacaa
      541 gcaaatttgg atctggcgat ttcgaaagct gtaactctga attggccccg cgagggaagt
      601 gtgcttgagg ttcgcggaga attcttcaac gcgttcaacg atgcgcagtt tgcgaatccc
      661 gacaccaact ttacgtcccc aactttcggc gtaattagca gaacgtctgt aaatgcgcgc
      721 gttgggcagc tagcgctgag gttcagtttt tagcgcggca aactcgcagt cgcatggaag
      781 catcgacgac aaggggctct gcatccggtc ctgaaatcac tgcaccttta ttggggtttc
      841 cgtcgctttc ttgctcttca gaagagcaag gaaataatgg ttgctttcat tgttgttcac
      901 catgtgccga cgtgaagaat gagtccgctg ctgaattgga gactgtgttg atagcttatg
      961 ccatttagat cgtttgtcca ggaatgcgag tattctaatt gagccacgcg gtaagaaagg
     1021 gcgctattga gtttcacgtc tattccgctc ccggcctgga cagtaaaacc gttcgtctcc
     1081 caatgtttag cgtactgagg gtagagtttg taagcaggtg tggacttcga ggctgacgac
     1141 ttgacggctt tttcgatgct agggtcgatg agctcctggg tgagcttgct gccaccaatt
     1201 aggacttcgg catgagttgt ccagcgcccg acgggatgag gagtccagcg tgctccggcc
     1261 agataggtga gcgagtcacc acttagattg tccccgagac caagcaactt acagccactg
     1321 acatcgacaa tgaactgcca ctccgaagct acacgaactg ctcctgtgcc tccgccgccc
     1381 acgcaaggtg cggcatgatg gtttcccagg taagtcttca cttttgctgt gaggttgaat
     1441 tcaaaagggg aaactcccgg cggcggcgag acctccattg gagtggtgtt gcgttttccc
     1501 aaagcttcca tgaattcaga atctccgagc tctgcggaaa gcacgcccgg tcgattcgca
     1561 cgataccagg gagtccttaa cgccacaacg ttggccattg agcgggcagg attcaaaccg
     1621 ccacgaacaa gcagtcgaac ccagttgttc gaggtgcgtc tttcgagata acggatgaag
     1681 tattgatcta gcgcgtcttc ggcaatcgac catcctaacc caatcgccgg agtaactacg
     1741 tgatccacaa atcctacttg tgggtagtag tgctggatat ttccgatcga tgcttcgcta
     1801 atcgggccaa tctcaaacaa gacgctgtaa acataggagt acgccgcgcc gcgcagtttg
     1861 cccttccaat acctgcggtt ttgtccaaac tgaatgtcgc ggtatgcgcg atcgttttgc
     1921 gtccagatat acccagtcac ggctccttgc atcgggtgac caacatagtt caccaagaaa
     1981 ggatcaccat cgctccagcc atgcaggttt cccaccgagt ttaggtaacc acggaaaaat
     2041 ggattggaga atccgtcgcg agttccttct tcggtggcac aacgaaacgc attttcaaag
     2101 ctcataaaga aaaaagactg tccaacgaga tggacccaat caactccgcg ctgttcttct
     2161 ttggagattc gcacaggctt gaggggctgc acacgagccg ccggtgaccc ggcatcggat
     2221 gcaggctgcg caccggcata caaagaaagg ccactagcct ggctttgcgc ttctgcacat
     2281 aacgcggtag caaagcccaa aattaccgtc acgattgcaa agagaattcg atgaaaagtt
     2341 gccatatcag ctggcattga agggtaccct ccagctaaca agagcccttt atcaccattg
     2401 gcataaactg gtagaaatac cagaacgaca gatctcagaa gagtgaataa caatagtacg
     2461 caaagagaac gaccttcggt gccaggtggg tgctggaggc gtaaaactta gttacaggcg
     2521 cgagtgaagt gccagagttc gcagagccct gggctcagaa cttccaggtt aaggccccgc
     2581 cacggaagat cttgaaagca gatccaatct tgcatgtact caccgccgcg agcatcttgt
     2641 ggccgagcaa tccggcaaat aggttagcgt gccgaatcgc accgcacagg attgttgacc
     2701 tctgggcatg gtcaactagg ttttgggttt gggcggaaag ttcttaagga gctttgcgac
     2761 cgctttggtg acgttcttct gcttcttttc gggtttcgcc ttgggatcga gcgtcttcgt
     2821 cgccactccc cgccaaacga gctgcttatt ggcagaatcg tacacactaa ggtcaagctg
     2881 gcccacgtat acggta
//
LOCUS       DASSFP010000438         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_874159, whole genome shotgun sequence.
ACCESSION   DASSFP010000438 DASSFP010000000
VERSION     DASSFP010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874159"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VFH91_09885"
     CDS             complement(<1..1646)
                     /locus_tag="VFH91_09885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6863350.1"
                     /translation="MKKVLFAFILASLTLCFSAVVASAQNTAGVTGVVKDGTGAVIAG
                     ADVNLTDTKTGAELTTKTNDQGVYSFTRVAPGIGYKLTFTSAGFQTLVINDVALGVGN
                     TETHNAEMTIGEVSGTVEVTASNEVTLNTTDASIGNVIGERRLKELPIQIRNSPAALI
                     GLQPGVVGNNVGTTTTNRVGSVTGSRADQGNITVDGIDANDQATGQFAATVGNAPIDA
                     IQEFRAVSTNPGASEGRSSGGQVELVTKSGSNEFHGNVREYNRTAATAANSFFNNRSG
                     IARPQLTRNQFGGSIGGPIMKDALFFFFDYEGRRDAQGVSYLRIVPLNHFRNGGLGYI
                     NNTAGCGTGSRLNTTPNCISVLTPAQVTALDPKGVGGNSSLLSFINSRYPVANDLTAG
                     DGINTGGFRFNAPSQRKDNTYTTRIDWNATSKQKFFGRFNIARRLQTDTVNSVAAQFP
                     GDPETAQIVVQDYAWVVGHTWTVSPSLVNQATVGVSRSGLLFPTNFKPSFPNSFTFGM
                     GLAAPFAGISDQDRFVLVPTIRDDVTWIKGTHTLEFGGSF"
     gene            complement(2146..>2832)
                     /locus_tag="VFH91_09890"
     CDS             complement(2146..>2832)
                     /locus_tag="VFH91_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6863351.1"
                     /translation="SNFNLRRGPIFTDILLADEINRTPPKTQAALLEAMEERQVTIDG
                     EGHQLPPIFTVLATENPIEYEGTYPLPEAQLDRFQLKILLGYPSEGEEREVLHRWNSG
                     FNSKRLDQVNLVPLADPMAIVKCRAEVAAAQMEPGVQHYAVELVRRTRSHPFVHYGAS
                     PRAAVALLLCSKALAAIRGREFVTPDDVRDVSLPVLRHRLTLRADAELDGVTPDAVVT
                     DILKLAEVPR"
BASE COUNT          712 a          710 c          717 g          693 t
ORIGIN      
        1 ttgaatgaac caccaaactc taaggtatga gttcccttga tccacgtaac gtcatcgcga
       61 atcgtcggta cgagtacaaa acggtcctga tcgctgattc ctgcgaacgg agcggcgagt
      121 cccataccga aggtaaacga attcgggaac gatggcttaa agtttgtcgg gaagagcaaa
      181 ccagaccgcg aaacgccgac ggtcgcttga ttcactagcg aaggactaac agtccaggtg
      241 tgaccgacta cccatgcata atcttgaaca acgatctgcg ctgtttccgg atcgcctggg
      301 aactgggctg caactgagtt aaccgtatcg gtctgaagac gacgagcgat attgaatcgt
      361 ccaaagaact tctgtttaga agtagcgttc cagtcgatac gagtcgtgta agtgttgtcc
      421 ttgcgttgtg aaggggcgtt gaaacggaaa ccaccggtgt tgatcccatc gcccgcggtt
      481 aagtcatttg caaccgggta acgactattg ataaagctca ataacgatga gttgccacca
      541 acccccttcg ggtctagtgc ggtgacctga gcaggagtga gtacgctgat gcagttaggg
      601 gttgtgttaa gacgcgaacc cgtgccgcaa cccgcggtat tattgatgta tcccaatcct
      661 ccgttgcgga agtggtttag gggaactatt cgcaagtaag aaacaccttg cgcatcgcga
      721 cggccttcat aatcaaagaa gaagaatagc gcgtccttca tgattggacc accaatgcta
      781 ccgccaaact gattgcgggt cagctgtggg cgggcaatgc ctgatcgatt attaaagaag
      841 ctgttagcgg cagttgctgc ggtacgattg tactcgcgaa cgttgccgtg aaactcgttt
      901 gagccgctct tcgttaccaa ctcaacctgg ccgccactgc ttcgtccttc ggaagcaccc
      961 ggattggttg agaccgcacg aaattcctga atagcgtcaa taggagcatt tccgactgtg
     1021 gcggcgaatt gaccagttgc ctggtcgtta gcgtcaattc cgtcaacagt gatgttgccc
     1081 tgatccgctc gcgatccggt aactgaacca acgcgatttg tagtcgtggt ccctacgttg
     1141 ttaccaacga cgccgggctg tagaccaatc aacgctgccg gcgagttgcg aatttgaatc
     1201 ggaagctctt tcaatcttcg ctctccgatt acgtttccga tactggcgtc tgtggtgttg
     1261 agggtaactt cgttagaagc cgtgacctca acagttcctg acacctcacc gatcgtcatc
     1321 tcggcattat gagtttccgt gttacctaca cctaatgcca cgtcattgat tacgagggtt
     1381 tgaaaaccgg cactggtgaa agtgagtttg taaccaatgc ccggtgcgac ccttgtaaaa
     1441 gaataaacac cctgatcatt ggttttagtg gtgagttccg cgccagtctt tgtatctgtg
     1501 aggttgacat cggcgcctgc aataaccgct ccggtgccgt ctttaacgac acctgttaca
     1561 cccgctgtgt tttgcgccga cgccactact gcagaaaaac agagcgtcag cgaagcgaga
     1621 ataaaggcaa aaagcacttt cttcatttgg actccttcca ggaaatgtta tttgaaatgc
     1681 gacctgaaga tggttgcgat tggcgagaac ccacccgggc cacaaagaac caacgtcaag
     1741 acacacgacg tccagttctt ctgaaaatta atttgagtgc ttgatcaccg agccttgttc
     1801 ttagccagtg gttagcagct cgtcaaaaag taaaacgaag ccgctccttc gcaggaggga
     1861 tagatgtctc acgatcgaga tcgagcccgt tcgtgaaata tctgtctgac ttgaggggag
     1921 attgcatgcg ggtctgctaa tgcaaggaat gttccgacac tcttgtcatg ctgactatct
     1981 aataaattgg cgaagagttc agaaaattga caaccatgca atggaagaaa atccaaaaat
     2041 caaaactaat ttccgaattc gtcaatcaac caggttcaca tggtccataa actcaaacac
     2101 tttgagcgtg ttcgaatacc cgtagatcta ctgaggcaaa ttttcctatc gtggtacttc
     2161 agcgagtttt aagatgtcag tcaccacagc atcaggtgta actccgtcta gttccgcatc
     2221 agctcgaagc gtgaggcgat gtcgtagtac gggcaaagaa acatctcgca catcatcggg
     2281 agtgacgaac tctcgaccgc gaattgcagc aagcgctttc gaacaaagta gcagggctac
     2341 ggctgcgcga ggactggcgc cgtaatgcac aaatggatga ctgcgtgtac gcctgacgag
     2401 ctcgaccgcg taatgctgca cgcctggctc catttgtgca gctgctactt cagctctgca
     2461 tttgacgatc gccattggat cagcaagcgg cacaaggttc acctggtcca gtcgttttga
     2521 attgaaacca gaattccaac gatgcagtac ttctcgctct tcgccctcgg acggatatcc
     2581 aagcaggatc ttcagttgaa atcgatcgag ctgtgcttcc ggcaaaggat aagtgccttc
     2641 atattcaatc gggttttctg tcgcgagaac tgtgaagatc ggaggcagtt gatggccttc
     2701 tccatcgatc gtcacctgtc gctcctccat agcttccagc agcgctgctt gagtttttgg
     2761 cggcgtacga ttgatctcat ccgccagaag aatatccgta aagattggtc cacgccgtag
     2821 attgaaattt ga
//
LOCUS       DASSFP010000439         5631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1624963, whole genome shotgun sequence.
ACCESSION   DASSFP010000439 DASSFP010000000
VERSION     DASSFP010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624963"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="murF"
                     /locus_tag="VFH91_09895"
     CDS             <1..1018
                     /gene="murF"
                     /locus_tag="VFH91_09895"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET6863352.1"
                     /translation="NYNNGLGLPLSVLRMVSEGRSPDQFDLAVLEMGMSSPTHEIRRL
                     CEITPPDVAVELMVAPVHLEYLGSLENIAAAKAELIEGMKPDGTAILNADDELVMKMR
                     EKHRGRTLTFGIENRADVRASEIDTTSLGKVTFSLQMPKGNIQATLPMSGRHNLSNAL
                     AASAVGVAFDMSPEQIRDALSTARPPKMRGEVFDFAPGFTLIDDSYNSNPRSLLNMVR
                     TMAESRKNRRRLIVLAGEMLELGPDEIELHKQAGRDIAAAGIDILWGIRGLAREMVSG
                     AKEQGLNNTKFFESSSDAAKEIVKEVREGDLILVKGSRGVATDKVVALLKENFPPTGE
                     DLRT"
     gene            1045..2199
                     /gene="mraY"
                     /locus_tag="VFH91_09900"
     CDS             1045..2199
                     /gene="mraY"
                     /locus_tag="VFH91_09900"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET6863353.1"
                     /translation="MIYYFLYEILYNHFKDKDWYLVKALNVFQYVTFRTAYATITALL
                     ISLFFGGKLIEALHKWNIGQLIREEGPQAHMVKRGTPTMGGVLIIGSVLISTLLWARL
                     SSLYVWMAILATTLFAAIGFMDDYEKMAKQRSLGLTGRQKLAAQLLIAIGVWVVLFIS
                     TRYWASDYSWNLVFPFFKFTSEPTGRTFIGPYLYLVLIVVVLLGSSNAVNLTDGLDGL
                     AISVTFIAMSALTGFTYLSSDLRWARYLDLTHRPEAAELTVFCGAMAGASLGFLWFNA
                     PPAQVFMGDVGSLAIGGAMGTVAVLTKQEFMLLMVGGVFVIEAFSVMLQVSVFKMTKR
                     TTGTGRRLFKMTPLHHHFEMQDWKEPKIVFRFLILAILFALLSLSTLKLR"
     gene            2236..3612
                     /gene="murD"
                     /locus_tag="VFH91_09905"
     CDS             2236..3612
                     /gene="murD"
                     /locus_tag="VFH91_09905"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET6863354.1"
                     /translation="MEVTGRKVLVVGAARSGIASAKFLMDRGAVVALHDQKPVEKWSQ
                     EALELKESGVGLVPGDVPTWLLDQLDLVVVSPGVPATIIPIRYAERAGAEVIGEVELA
                     SRYLKGRIVAITGSNGKTTTTSLIGDLLKNGGLKVQVGGNIGRPLISMVEQSTDDGWT
                     VVELSSFQLETIKSFHPTIAVVLNVTPNHMDRYETFNDYAGAKHRIFMNQTVNDTAIL
                     NADDPTVSSWRPGLKARVLTFSVKTEVNEGIFLRGNTLILRSGKAEQALIDVTEMKLR
                     GTHNAENVAAALAVGIACGIGADSMRETVKAFDPVEHRLEFVDEIQKVRFYNDSKATS
                     VDATLKALEAFASDPGKVVLILGGRGKKAPYAPLAELIRNKVRSLILIGEDAETISSE
                     LGHLAPVERASDMKDAVNRSFVAAKSGDTVLLAPACASFDMFDSFEHRGKVFKSEVFE
                     LRSRSAEN"
     gene            3620..4813
                     /gene="ftsW"
                     /locus_tag="VFH91_09910"
     CDS             3620..4813
                     /gene="ftsW"
                     /locus_tag="VFH91_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET6863355.1"
                     /translation="MARKLVPDKWLFASTVGLALFGVVMVYSASAVMAEKENGNQFYY
                     VIKQGMWVAIGFMVMILAMQFNYQQLKSRRVVYGLLIISTISLFAVFGFSSTNGAHRW
                     LKLRAFSVQPSELSKLALAIFLAYFLEKRAGEEGDFWRTFLPCGLVTGWLAVLVVVEP
                     DFGTALMLALIFAVVVYTAGARLAHLAMVAAPALVAAAGLLIFVPWRMRRLVTFMDPW
                     ADQQGAGFQVVQSLIAIGSGGPNGLGFAQGKQKMLFLPFAHSDFIFAVIGEELGLVGA
                     LTVVAVFALFLWRGVRAALLAPDRFGMLLSLGIVTSIVAQALFNISVVLSLVPTKGIP
                     LPFISYGGSSLVPTLAAVGILLNISQHSSGSLDVGLREPNKGRARERSNLSVATAGGN
                     ADTRF"
     gene            4820..>5631
                     /locus_tag="VFH91_09915"
     CDS             4820..>5631
                     /locus_tag="VFH91_09915"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET6863356.1"
                     /translation="MRVLIAAGGTGGHIYPGIAVAQEIMRRNPQSSVRFVGTARGLET
                     KLVPKAGFDLSLIESAGLKNVRAVARVKGLATLPGSFVSAWKLIRQFQPDVVVGAGGY
                     VSGPVVLMAALTNHRTLVMESNALPGWTNRVLARFVDRAAVSFEASLAYFRGKGVVTG
                     NPVRREFFEIPLRTIQPEQLSLLVFGGSQGAHAINEAMIAALPLLREKGVTVQVKHQT
                     GPADFERVQAAYVTAGWQGNSTVCSYIDNMVEDFGVADLILCRAGATTTAEL"
BASE COUNT         1298 a         1283 c         1595 g         1455 t
ORIGIN      
        1 gaattacaac aacggactcg gacttccgct gtcggtctta aggatggtga gtgaaggaag
       61 atcaccagat caattcgatc tggcggtgct tgaaatgggt atgtcatcgc cgacacatga
      121 gatcagacgt ctctgcgaga tcacgccgcc cgacgttgcc gtcgagctaa tggttgcgcc
      181 cgtgcacctt gaatatctgg gctcgctcga aaacattgct gccgctaaag ccgagctgat
      241 tgagggcatg aagcccgatg gcaccgcgat attgaacgct gatgacgaac ttgtgatgaa
      301 gatgcgtgaa aaacatcgcg gccggacact tacatttgga atcgagaaca gggctgatgt
      361 gcgagccagc gaaattgata cgacatcatt aggcaaggtt actttttcgc tgcagatgcc
      421 gaaaggaaac attcaagcca cgttaccgat gagcggacgc cacaatctta gtaacgctct
      481 cgcggcttcg gcggtgggag tcgcttttga catgtcgcca gagcaaattc gggacgcgct
      541 cagcactgcc aggccaccga aaatgcgtgg tgaagttttc gattttgctc cgggattcac
      601 gttgatcgac gattcataca attcgaatcc gcgctcgcta ttgaacatgg taaggacaat
      661 ggctgagagc cggaagaatc gacgacgctt aatcgtgctg gccggcgaga tgttggaact
      721 tggacctgac gagatcgagt tacacaaaca agcggggcgc gacatcgcag ccgctggaat
      781 cgatattctt tggggaataa gagggttggc gcgcgaaatg gtttctggcg caaaagagca
      841 gggactaaac aatacgaaat tcttcgagtc atcttcagac gccgccaaag aaattgttaa
      901 agaagtcaga gaaggagatt taattcttgt aaaaggatca cgcggcgttg caactgacaa
      961 agtggtcgct ttacttaagg aaaactttcc accaactgga gaggatctga gaacctaaac
     1021 attattaacg accggcagac atagatgatc tactatttcc tttacgaaat tctctacaat
     1081 cactttaaag ataaggattg gtacctcgta aaggctctga acgttttcca gtacgtcacc
     1141 tttcgcacgg cttacgccac gattaccgct ctcctcatct cgctgttttt cggtggtaaa
     1201 ttgatcgaag cactgcacaa gtggaatatc ggacaactga ttcgagaaga aggtccgcag
     1261 gctcacatgg tcaagcgcgg gacgccgacc atgggcggtg tgctcataat cggttctgtt
     1321 ttgatttcga ctttgctgtg ggcgcgactg tccagccttt atgtatggat ggcgattttg
     1381 gcgacgacgc tctttgcggc gatcggtttt atggatgact acgaaaagat ggccaagcag
     1441 agaagtcttg ggctaacggg aaggcagaag ctcgcggccc agttgttaat tgcgatcggt
     1501 gtctgggtcg ttcttttcat ctcgacgcgc tattgggcca gcgactactc ctggaattta
     1561 gtttttccgt ttttcaagtt tacttccgag ccaacgggca gaacctttat cggtccttat
     1621 ctttatctgg tcttaatcgt ggtcgtattg ctcgggtcct cgaacgcggt gaacttaaca
     1681 gatggtctgg acgggctcgc gatcagcgtc acattcatag cgatgagtgc cctaactggg
     1741 tttacgtacc tgagcagcga tttacgttgg gcccgttatc tcgacttaac tcatcgtcca
     1801 gaggcggctg aactcactgt tttttgtgga gcgatggccg gagcaagtct cggatttctt
     1861 tggtttaacg cgcccccggc ccaggtattc atgggtgacg tcggaagttt agcgatcggt
     1921 ggtgcaatgg gaacggttgc tgtactgaca aaacaggaat tcatgttgtt gatggtgggc
     1981 ggagtatttg taattgaagc gttctcagta atgcttcaag tgagcgtctt caaaatgacc
     2041 aagcgcacaa cggggactgg gcgccgtctt tttaagatga ctccgctcca ccatcacttc
     2101 gaaatgcagg attggaagga gccgaagatc gtctttcgat ttctaattct agcgattctc
     2161 tttgcgttgc tgagtttgtc aacgctcaaa ctacgttaat gctcgagttg tgaggatgct
     2221 tgtggcagtt accttatgga ggttacggga cgaaaagtac ttgtcgttgg tgccgcgcgg
     2281 agtggaattg cctccgcgaa atttcttatg gaccggggag cagtcgttgc gcttcacgat
     2341 caaaagccag ttgagaaatg gtcgcaggag gctttggagc tgaaagagtc aggcgtcgga
     2401 ttggttccag gtgacgttcc gacctggctc ctcgatcaat tggatcttgt agtggtgagt
     2461 cccggtgttc cggcaaccat tatcccgatt cgatatgcag agcgagcagg cgcagaggta
     2521 ataggcgaag tcgaactggc ttccagatat ttgaaaggcc gaatcgtcgc gatcaccggc
     2581 agtaatggaa agacaacgac gacatcattg attggagacc tgttaaaaaa tggtggcttg
     2641 aaggtccagg tcggcggaaa catcggtcgg cccttgattt caatggtcga gcagtcaaca
     2701 gacgatggtt ggacagttgt cgagttatca agtttccaac tcgaaaccat taagtcattt
     2761 catccgacaa tcgcagtagt actgaatgtc acaccaaacc acatggaccg ttacgagacg
     2821 ttcaacgact atgcgggagc taagcaccgg atctttatga atcaaacagt gaacgacacg
     2881 gcgatcttga acgcagacga tccaactgtc tcgtcgtggc gtcccggtct taaagcaaga
     2941 gttttgactt tcagcgtcaa gacagaagtc aacgaaggca tcttcttgcg aggcaatacc
     3001 ttaattcttc gatcgggaaa agcagaacag gctttgattg atgtgacaga aatgaagttg
     3061 cgcggcactc acaacgcaga gaatgtggca gcggccctgg cggtgggaat agcgtgtggc
     3121 atcggggccg actcaatgcg tgagacagtg aaggcattcg atcccgtcga gcatcgcctt
     3181 gaatttgttg atgagattca aaaggtcagg ttttacaacg actcgaaagc cacgagcgtc
     3241 gatgcaactc tgaaagctct ggaagcattc gcgagcgatc cgggcaaagt tgttttgatt
     3301 cttggagggc gtgggaaaaa ggcgccctat gctccgctgg ccgaattgat tcgcaacaag
     3361 gtcaggtcac tgattctcat aggcgaagac gctgagacaa tttcttctga gctcggccat
     3421 cttgctccag tggaaagagc gagcgatatg aaagacgcag tcaatcgttc gttcgttgct
     3481 gctaaatcgg gtgataccgt cttactcgcg ccagcgtgtg cgagtttcga catgttcgat
     3541 agttttgaac atcggggcaa agtctttaag tctgaagtct ttgagctcag atcgcgatcc
     3601 gcagagaact aaatttttta tggcacgtaa gcttgttccg gataaatggc tgtttgcttc
     3661 gactgttggt cttgcgctgt ttggcgttgt gatggtctac agcgcttctg ctgtaatggc
     3721 tgaaaaggag aacggcaacc agttttacta cgtgatcaaa caaggtatgt gggtggcgat
     3781 cggtttcatg gtgatgatac tggcgatgca attcaactat cagcagttga agagtcgccg
     3841 cgtcgtctat ggtctattga tcatttccac gattagtctt ttcgcggtgt ttggcttcag
     3901 cagtacgaac ggagctcatc gctggctgaa gttgagagcc ttctcagttc aaccttccga
     3961 gttgtcgaaa ctggcgctcg cgatattcct cgcttacttc ctcgaaaagc gggcaggcga
     4021 ggaaggagac ttctggcgga cctttttacc gtgtggatta gttaccggtt ggctcgcagt
     4081 gctcgttgtg gtcgagcctg attttggcac tgctttaatg cttgctctga tttttgcagt
     4141 ggtcgtgtac acggccggcg cacggttggc ccatctcgcg atggttgccg cgcccgcttt
     4201 agttgcggcg gcagggttat tgatttttgt tccgtggaga atgaggcgac tggtaacgtt
     4261 catggatcca tgggccgatc aacaaggcgc agggtttcag gtggttcagt cattgatcgc
     4321 gatcggttcg ggtggtccca acggattggg ctttgcacaa ggcaaacaga agatgctctt
     4381 tcttccattt gcccattcag atttcatctt cgcggtcata ggagaagagt tgggcctcgt
     4441 cggagcactt acagttgttg ccgtcttcgc actctttctc tggcgcggag ttcgtgctgc
     4501 gctgttggcg ccggaccgct ttggtatgtt gctcagtttg ggaattgtta ccagtatcgt
     4561 cgcccaggcg ctctttaaca taagtgtcgt actttcgctc gttccgacta agggcattcc
     4621 gcttccgttt atttcatacg gaggttcatc gttggtcccg acgctggcag ccgttggaat
     4681 acttttgaac atttcccagc attcaagcgg atcacttgat gttggtctac gtgagccgaa
     4741 caagggtaga gcgcgagagc gtagtaattt gagtgtggca actgcgggtg gaaatgctga
     4801 cactcgtttt tgaaaaccga tgcgcgtgct gattgctgcc ggaggaactg gcggacacat
     4861 ttatccgggt attgcagtcg cgcaggagat catgcgacgc aatccgcagt cgtcggtccg
     4921 tttcgttggt acggcgcgcg gtttggaaac caagctcgta cctaaagcag gttttgattt
     4981 atcgctcatt gaaagtgctg gtctaaaaaa cgttcgagcc gtggcccgag tgaaaggcct
     5041 ggcgacgttg ccaggaagtt tcgtttccgc ctggaagcta attcgacagt tccagccgga
     5101 tgttgtggtt ggggcaggtg gttatgtttc tggtccagtg gtgctgatgg ccgcgttaac
     5161 taatcatcga acgctcgtaa tggagtcgaa tgcgttaccg ggttggacca atcgcgtttt
     5221 ggctcgcttc gtagatcgtg ctgcggtctc ctttgaagct tcactcgcgt atttccgggg
     5281 caaaggagtg gttacgggaa atccagtacg gcgtgagttc tttgaaattc ctttgcggac
     5341 cattcaaccc gagcagcttt cgttgctcgt gtttggtggg tcacaaggag cacatgcaat
     5401 taacgaagcc atgattgcag ccttgccgtt actcagggaa aaaggggtaa ccgtacaggt
     5461 taaacatcag acaggaccgg ctgacttcga acgagtccag gcggcttatg taacggcagg
     5521 ctggcagggc aattcaacgg tgtgtagcta catcgacaac atggttgagg atttcggggt
     5581 ggctgactta atactctgcc gggcgggcgc cacaacgacc gccgaattaa t
//
LOCUS       DASSFP010000440         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1764710, whole genome shotgun sequence.
ACCESSION   DASSFP010000440 DASSFP010000000
VERSION     DASSFP010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764710"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFH91_09920"
     CDS             complement(<1..1003)
                     /locus_tag="VFH91_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6863357.1"
                     /translation="MRTLSPRVAFLLLSLATCALSVSAVLAFNSSNQPLVSETTVGTE
                     GFAAVQSATLLQPTNTTSTHSSSNNHGPPFWDRFKFRIIVVACFVIFQSILITGLLVE
                     RSRRWRASRELAECEERYRNLLETQTELICRYLPDTTLTFVNDAYCRFFEKSRQQLVG
                     SRFLELLPEEARNPTQKHIRRLMENSLSVIYEHEVLLPDGTIGWQQWTNHVIVRTDGI
                     VELQGIGRDVTQQKRAETALRDAEERNLAILRAIPDLIFLQDKDGVYLDYHPKDYQHL
                     VVPAEKFLGKQMTDVFPPEIEVPMRKNLTRAISTGNLQLSEYSIEFEGKRKWFEARTV
                     "
     gene            2087..>2584
                     /locus_tag="VFH91_09925"
     CDS             2087..>2584
                     /locus_tag="VFH91_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863358.1"
                     /translation="MSTVTTGPLPSWREGATRQAIISFVNRITNQSSSDFVTPEDRIA
                     VFDNDGTLWCEKPVPIQADFLFRRIGEQAKKNPELCDKQPWKAVSEKDYRWLSDVITK
                     HYNGDDSDLNVMSAGLIQAYAGESIETFAEKAGAFLNSSENPALKRPYLGTAYTPMVE
                     LLRYLK"
BASE COUNT          673 a          631 c          667 g          613 t
ORIGIN      
        1 gaacggtgcg cgcttcaaac catttgcgtt taccttcaaa ctcgatcgaa tactcggaca
       61 gttgtaagtt tcccgtgctg atagcgcgag tgaggttctt gcgcatgggc acttcaatct
      121 ccggcggaaa tacgtcggtc atctgtttgc ccaggaactt ttccgcaggc acaaccagat
      181 gctgataatc cttcgggtgg tagtcgagat agaccccatc tttgtcctgc aggaaaatca
      241 aatcgggaat cgctcgaagg atagcgagat ttctttcttc agcatctcgc agcgcggttt
      301 ctgccctctt ttgttgcgtg acgtctctgc cgattccctg tagttcgaca attccatcgg
      361 tccgaacgat gacgtgattg gtccattgct gccagccaat cgttccgtca ggtaataaca
      421 cctcgtgctc atagatcacg gatagagagt tctccatcaa tcgtcgaata tgtttctggg
      481 tcgggttgcg agcttcctct ggcaacaatt ccaggaagcg tgagccgacc agttgttgtc
      541 gcgatttctc aaaaaaccgg cagtaagcat cgttaacaaa agtcagcgtt gtgtcaggca
      601 agtaacgaca gatcaactca gtttgtgtct cgagtagatt ccggtaacgt tcttcgcatt
      661 cagcaagttc gcggctggcg cgccaacgtc tgctgcgttc aaccagcaga cctgtgatca
      721 agatcgattg aaagatgacg aagcaggcaa cgacgataat tctaaattta aagcgatccc
      781 aaaaaggtgg accatgatta tttgaactag agtgagtaga agttgtgtta gtgggttgca
      841 gtaaggtagc agattgaact gctgcgaaac cttcggtccc aacggtggtt tcggatacta
      901 aaggttggtt ggatgaatta aacgcaagaa ccgcagaaac cgacagcgca cacgttgcga
      961 gactgagaag tagaaacgcg actcgtgggg aaagcgttcg cataattgcc gcgcaaaggg
     1021 ccggtttggc tcaaatgact cattactgtc gttaacccct atatagttcg cggagtaggg
     1081 gcactttagg gggaagctct gggcccttga ttgtctgtca cacggcagtt gatttgaact
     1141 tcagctgctc gattctgaga tatctccaga gcagggtgga cacgagtccg ttgaaaattt
     1201 aagggtcgtc ctcgtcgtcc ttagtcgctc ttttcttgtt cagctatggg cggagtgtac
     1261 gctcggtctc tcatactttc aaccccgaaa aattactgta gctggccgaa aataacccgt
     1321 gaatcactta ctactaagcg tcgtagtcgt cgagatcaaa aagtaagacg cgagccagta
     1381 caaaaccgcg actgtccgat atgctttaga gccagtggca aaaatccctt tcattctccc
     1441 cgagcttcag ctcggggttc agagcgaact cgagtcaatg aaccgtttca acggtttacc
     1501 cagggcgagc aagaggtgcc accggagtca cgcgacggag taagcgcact ctcgccggaa
     1561 tttgtgacac cggcttgctt tctgcggaac cgaacaaaga gggcgcgcgg aaaaccgtta
     1621 aaacggttcg cctctcttca tcggcgaata accccgagct gaagctcggg gagaatgaga
     1681 tggacttttg caagaggctc ttaaaagcat atcggacagt cgcggttcag tactggctcg
     1741 tgggtcggct aaaaccgact ttttgtgcaa agcgagccca gattaacgag gtgagatgat
     1801 gattcttcac cattactaca gatcctcccg ccacgaaata cgcatttcac ctgattaccg
     1861 catggagttc aattacttaa ccttgcgaat cactcaagac agaaagagac cctcgaaatc
     1921 acccatactg aggacaagtg ggtagcagtt tgcgccgttc ctaagaggtt aaagacaggc
     1981 gaaatttgac gaagtctatc gcaatagctt ggcgcaactt taacggagac gtgtcgcttg
     2041 gagaatcgag gttcagaact cgagcacgtg gagggggaaa atcatcatgt caacagttac
     2101 gaccggacca ctgccatcct ggcgcgaggg tgcgaccagg caagccatca tcagtttcgt
     2161 caaccggata acaaatcaaa gctcgtccga ctttgttacg cccgaagacc ggatcgcggt
     2221 tttcgataac gacggaactc tttggtgtga aaaaccggta ccaattcaag ccgactttct
     2281 atttcgacgg ataggtgaac aggcaaaaaa gaatcccgaa ctctgcgata aacagccttg
     2341 gaaagcagtc tcggagaagg actacaggtg gttgagcgac gtcatcacca aacactacaa
     2401 cggcgacgac agcgatttga atgtgatgtc ggccgggtta attcaagctt acgccggcga
     2461 aagtattgag acgttcgctg aaaaggctgg tgcctttctt aactcttcag agaatcctgc
     2521 gctcaaacgc ccgtacctcg gaacagccta cacgcctatg gtggagctgt tgcgttatct
     2581 gaaa
//
LOCUS       DASSFP010000441         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_132364, whole genome shotgun sequence.
ACCESSION   DASSFP010000441 DASSFP010000000
VERSION     DASSFP010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132364"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1117
                     /gene="purM"
                     /locus_tag="VFH91_09930"
     CDS             83..1117
                     /gene="purM"
                     /locus_tag="VFH91_09930"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET6863359.1"
                     /translation="MTKSISYSDAGVDIDAATRTTDRIKELARTTFNQRTLSEIGSFG
                     GMFDGAFPSMSQPVLVASADGVGTKLKIAFATGVHHTVGRDLVNHCVNDILVQGARPL
                     FFLDYIATGALSPATVASVIEGVATGCRENGCVLLGGETAEMPGFYQSGEYDIAGFIV
                     GVVDRKKIIDGKGIVPGDLLLALPSVGLHTNGYSLARKLFFEVAGYEAQTNLPTVGMT
                     VAEALLQPHLSYLKPLDGLLDGGLIRGLAHITGGGLTDNVPRILPEGTAVVIEKKSWP
                     NLPLFELMQQLGNVVDDEMYRTFNMGVGMVIVCAPESAALIEKHFESIDQKCYRIGRV
                     VEGDRAVTLV"
     gene            1131..1769
                     /gene="purN"
                     /locus_tag="VFH91_09935"
     CDS             1131..1769
                     /gene="purN"
                     /locus_tag="VFH91_09935"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET6863360.1"
                     /translation="MDQNQKRQKPSRIAILISGRGSNMVALSDSIRNGVIANAEIVVV
                     VSDKADAPGVNLARERGLNTLVIERKARKREEHEADVIDALQQCNVDLVCLAGYMRVL
                     SHRFVETFPLRILNIHPSLLPSFAGLKPQLQALEHGVKLSGCTVHLVDDALDGGPIIA
                     QRAVPVFDDDSEETLSARILEQEHVLYAEAVNTIINNDYRVEARRVIIDRPQ"
     gene            complement(1887..>2652)
                     /gene="proC"
                     /locus_tag="VFH91_09940"
     CDS             complement(1887..>2652)
                     /gene="proC"
                     /locus_tag="VFH91_09940"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET6863361.1"
                     /translation="QIVGSHPREARRSQLSERYGIKMVEGNRDAVLSIHPADAEKGSV
                     VLLGVKPQRLNTVLSELKGSLQPEQVVVSIIAGITIEKISDDLHHDSVVRAMPNTPAQ
                     VGAGVTAWTATPVVSELQAEQVDAMFSALGKAIRVENERQIDMATALSATGPTYIFLV
                     MEALIDAGVHLGFSRHVAKDLVQEMMLGSVLFARESLKHPAELRNMVTSPGGTSAEAI
                     YQMEKGSLRTVLSKAVWAAFQRAEALGASSSKKPKS"
BASE COUNT          631 a          640 c          685 g          696 t
ORIGIN      
        1 gacgactctt caaggtcagg ctttatcgct tatggcgatt tcggcccacc acctaaaccc
       61 aggaagcagt agatcgactt agatgactaa atctatttct tatagtgatg ccggtgttga
      121 tatcgacgcc gcaacgcgaa cgaccgatcg gattaaagag ctcgcgcgaa ctacgtttaa
      181 ccagcgaacc ctttcggaga ttggcagttt tggcggcatg ttcgacggtg cgttcccgag
      241 catgtcccaa cctgtgctgg tcgcgtccgc agacggggtg ggaaccaaac tgaagatcgc
      301 atttgcaacc ggtgttcatc acacagtcgg gcgagacctg gtcaatcatt gtgtcaacga
      361 tattctggtc cagggcgcgc ggccgctctt ctttcttgac tatatcgcga cgggtgcact
      421 ttcacctgca acagtagcaa gcgtgatcga aggcgttgcc actggttgcc gtgagaatgg
      481 ttgcgtactt cttggcggcg agacggctga aatgcccggg ttctaccagt caggtgaata
      541 cgatatcgcc ggctttattg tcggagttgt tgatcgaaaa aaaataatcg atggaaaagg
      601 tatcgttccg ggagacttgt tgctggcttt gccgtcagtt ggtcttcaca caaacgggta
      661 ctcgcttgca cgcaaacttt tttttgaggt cgcaggatat gaggctcaaa cgaacctacc
      721 aaccgtggga atgactgttg ctgaggctct gctgcaaccg cacttgagtt atctcaaacc
      781 gctcgatggt ctgctggatg gaggtttgat cagggggttg gcccacatta caggtggagg
      841 gttgactgat aatgtcccaa ggattttgcc cgaaggcacg gctgtagtta tcgaaaagaa
      901 gtcctggccc aacttgcctc tcttcgagtt gatgcagcaa ctcggtaatg ttgtcgacga
      961 cgaaatgtat cgcaccttta acatgggtgt cggaatggtc attgtctgcg ccccggaatc
     1021 agctgcgctt atagaaaaac actttgaaag catcgatcag aagtgttacc ggatcggtag
     1081 agtcgtagag ggcgatcgcg ccgtaacttt agtttaggac gtcaatctaa ttggaccaga
     1141 atcaaaagag acagaaacca tcccgcatcg ctattctcat ttccggacgt ggatcgaaca
     1201 tggtcgcgtt atccgactcg ataagaaatg gtgttattgc aaatgcggaa atcgtagttg
     1261 tagtgagtga taaggctgat gcgccaggcg ttaatttggc tcgagaacgc ggactaaaca
     1321 cgcttgtaat tgaacgtaaa gctcgaaaac gtgaagaaca cgaagccgac gttattgacg
     1381 ctcttcagca gtgcaacgtc gatttggttt gtttggcggg atatatgaga gtgttgtcgc
     1441 accgttttgt agagacgttc ccgctgcgaa ttctgaacat tcacccaagt ttgttgcctt
     1501 catttgcggg cttgaaaccg caactgcaag cgcttgagca cggagttaaa ttaagcggtt
     1561 gcaccgttca cttagttgac gacgcacttg atggtggtcc gatcatcgcc cagcgcgcgg
     1621 ttccagtttt tgacgatgat tccgaagaga cactgtcagc gcgcatactc gaacaggaac
     1681 acgtccttta tgcggaagcg gtcaacacga ttatcaataa cgactatcgt gttgaggctc
     1741 gccgcgtaat aatcgatcga cctcaataac cttttatttc ctgaagtcag aagaagctct
     1801 ttcatctcga ttaccagtct tgtctttgcg aattccgttt atgatcgtcc aatgaacaca
     1861 taccgcaaag acgcgatttg tggatatcag ctctttggtt ttttcgagct cgaggctccg
     1921 agagcttcgg ctcgttggaa agctgcccaa actgcctttg ataggacagt ccgtaaactg
     1981 cctttctcca tctggtaaat cgcttccgcc gaagtcccac ctggcgaagt aaccatgttt
     2041 ctgagttcgg ctggatgctt aagtgattca cgtgcgaaca ggactgaacc cagcatcatc
     2101 tcctgcacca aatcttttgc tacgtgtcgt gaaaacccta aatgcacacc agcatcgatt
     2161 agcgcttcca tgactaagaa gatgtaagtt ggcccagtcg cgctcagtgc ggttgccatg
     2221 tcaatttgtc tttcgttttc tacacggatg gctttgccaa gcgcggaaaa cattgcatca
     2281 acctgttctg cctgaagttc tgagactacg ggcgtagcgg tccatgccgt tacaccggct
     2341 cccacttgtg ccggcgtgtt gggcattgcg cgtacgactg aatcgtgatg caagtcgtca
     2401 gaaatttttt cgatcgtgat gccggctatg attgaaacaa caacctgttc aggttgaagc
     2461 gaacctttta gttcgcttaa cacagtattg agtcgttggg gttttactcc aagcagaaca
     2521 actgaacctt tttcagcgtc ggcaggatga atggacaaaa cggcgtcgcg gtttccttcg
     2581 accattttga ttccatatcg ctctgacaat tgactgcgcc gagcttcccg ggggtgactg
     2641 cccacaattt ga
//
LOCUS       DASSFP010000442         5822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1764950, whole genome shotgun sequence.
ACCESSION   DASSFP010000442 DASSFP010000000
VERSION     DASSFP010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764950"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFH91_09945"
     CDS             complement(<1..230)
                     /locus_tag="VFH91_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863362.1"
                     /translation="MKTSILVLSIALLLFQGNAAYKLETRYPVPGNGGFDYVTIDSSM
                     RRLYLSHGTQVDVVDADSGKLIGTISETPGVHG"
     gene            complement(276..3320)
                     /locus_tag="VFH91_09950"
     CDS             complement(276..3320)
                     /locus_tag="VFH91_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6863363.1"
                     /translation="MGIASVVSNQSRAIVVLVGLLCAAGLYAAWQLPIAVFPQTDFPR
                     IVIIVDNGEAPASQTLVSVTRPIEEAMNGIPGIARIKSKTARGSSEINLYFDWNGNII
                     QELQLVQARMSQLLPTLPQGAQIRNIERLTFAVFPVTGYSLTSDRRDASTLRDLANYI
                     VRPQLARLPGVAVVGVAGGKTREFHVTIDPDRLTAHNVSAQQVVEAIRGANIIVSPGL
                     IEENHQLELALISGQAKRSDELNNIVVATVNNAPVRVADVATVSAGVEPEYTIVTADG
                     HPAALVNINRQPDANTVAVVDEVKAALSSMQGQLPKDVRVAAYYDQSLLVRESINSVR
                     DSILIGLLLSVVILFAFLRNWGTTFVAILVIPVTVLVTFLAMWLASLSFDLMTLGGVA
                     AAIGLVIDDAIVVVENIYTHLGRGQSRREAVQSAVSEITIPIIGSTITPVVVFLPLTL
                     LTGVTGVFFRSLALTMTVALLTSLVLALSFTPVLAERFVKAKPEGTNEETHGRFLGAI
                     ISRYESLLSFALDNKWIVVATIVIVLAGSYSIYGLLGSEFLPEFDESAFVLDYRAPPG
                     SSLAETDRMLQHVEEMLLKTPEIESYSRRTGIEMGLFVTEPNTGDFAVKLKPGHKRST
                     ADVITDLREQIAESEPSLDVEFVGILPDVIGDLQGNPEPIEVKLFSEDANALQAEADQ
                     VEASIKKVSGVVDTLNGVVISGPAVTFKVDPLRAAQFGVTSSDIANTITTAMSGEAAS
                     SILQQDRLITVRVIFPADVRTSLDKVKALQVRSSAGQLFRLDQVADVEYDKGQAEIER
                     ENLRQMVAVTGRLEGRDLGTAVREIQSQLARDIRLPPGMTVEYGGLYQEQQSSFRELA
                     MALVLAVVLVFITLLIEFRSFAHPIAIVTGAVLALSGVLLALLITRSTLNVVSLMGMI
                     MIVGIVAKNGILMLDAVEEHLAAGDNLRQALLRSGRRRFRPVLMTSLAAMLGMLPLAL
                     AIGAGSELLQPLAIAVIGGLTVALGLSLIVTPTVYAMLRTDG"
     gene            complement(3348..4739)
                     /locus_tag="VFH91_09955"
     CDS             complement(3348..4739)
                     /locus_tag="VFH91_09955"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6863364.1"
                     /translation="MSQDQNDIQEKESSTTSFEDEVQRARRARIVRQRNIVLVTIAVL
                     GLVLLTFLIWRWRKSSKENEAEITPTVGVKVIKAEKGPIAAEISAIGTIWPREKADVA
                     AKISAQIKQMALLKNKVVRAGEVIAVLESRDLQAQRAEAAGALNEARANERSFVSGTI
                     PKTNAEDQKALLDARARVNNTRATYERRRALFANGGISKKDLEAAQLELTTAEDELRL
                     QEQTVSLRARSLNPNDRALAAAKTAQAEQRLATLDAQLSYSVIRSPITGIVTDQFQYE
                     GDFASAGGKLVTIADSSSVIVKAPFSDTAAAQLKTGDAATVLPTDSSSEERKGQITLL
                     SRSTDPTSRTVEVWITLGNQDGKLRPNGAAQVTVFANSKDDAIVVPLSAVTLESSNAD
                     EGTVMVVDAQNVAHETKVTAGIRTAEKVEIVEGLKGGETVVVEGNYSLPDGTKVEIAT
                     DEKENNEEKDKKE"
     gene            complement(4749..>5822)
                     /locus_tag="VFH91_09960"
     CDS             complement(4749..>5822)
                     /locus_tag="VFH91_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6863365.1"
                     /translation="SPSLGLPPGEPRTFSFIANNAINEYVAFLSVSGDFDIAGRLRAT
                     VARNRALLAAAHAGTEVARRALAQAVIEGYYGLALASAQRQAAEGSLTSAEEFEHITS
                     LLLSGGEVASVDLTRAQLQTLSRRDELERARTNEEAAAGALRVLVGFEFTRPITTTDL
                     SLAIPTSAEDRQFKASEISNRPEFIQFDQLLLAAQQDIKVARAERRPSLSYSFNGGFD
                     SDSLRNPLLKEHSGVSAQISLTIPIFDWGTSRSKEAQARLRANLAQNERAMALRGFTQ
                     QFYAARAQTSGAANRIDIARQGVIKAESNVASSIARYRAGEAQIVEVTDAQTTLVAQR
                     SALYQAIFDYQVALARLKQSTGH"
BASE COUNT         1352 a         1553 c         1460 g         1457 t
ORIGIN      
        1 ccatgaacac ccggcgtctc ggaaatcgtt ccgatcagtt ttccactatc ggcatctact
       61 acgtcgactt gtgttccatg cgacagatat agtcgtcgca tcgagctatc aatggtaacg
      121 tagtcaaaac ctccgttacc cggaactgga tatcgcgtct ccagcttgta agcagcgttg
      181 ccttgaaata aaagaagtgc gattgataaa acgagtattg aagtcttcat aattcttgac
      241 gtcctctctg aattaggggt ttggccgtta caaagttaac cgtctgttct cagcatcgcg
      301 tagaccgttg gcgtaactat taacgacaag ccaagcgcta cggtcagacc tccgatgact
      361 gcgatcgcca atggttgcag caactcggag ccagcgccga tcgccaaagc caacggcagc
      421 attccgagca ttgctgctaa agaagtcatc aacactggtc taaatcttcg tcgcccggat
      481 cttaaaaggg cttgtcttag gttatcgccc gcggcaagat gttcctcaac ggcatcgagc
      541 atcaggatcc cgtttttggc tacgataccg acaatcatga tcattcccat taacgaaact
      601 acatttagag tcgatctagt gatgagcagt gccagcaaca ctccgctcag cgcgagtacc
      661 gcaccggtga caatcgcgat gggatgagca aacgaacgaa actcgattaa gagagtgatg
      721 aatacaagca ctaccgccag aacaagagcc atcgcaagtt ctcggaagct ggactgttgc
      781 tcctggtaca gaccaccata ttcgaccgtc atgcccggcg gcagtcttat atctctggcg
      841 agttgactct gaatttcacg cacagctgtt ccgagatccc ttccctccag acgtccagtt
      901 actgcgacca tttgtcgcag gttttctcgc tcgatctcgg cttgtccctt gtcatattca
      961 acatccgcca cttgatccag acgaaagagt tgtcccgcgg aagaacgcac ctgcaaagcc
     1021 ttaactttat cgagtgatgt ccggacatcg gccgggaaaa tgacgcgcac cgttatcaag
     1081 cggtcctgct gcaaaatcga agaagcggcc tcaccagaca tggcggtcgt tatcgtgtta
     1141 gcgatatcac ttgaggttac gccgaattga gccgctcgca aaggatcaac cttgaaagtt
     1201 actgcgggac cactgattac gacgccgtta agggtatcga caacccctga taccttcttg
     1261 attgaggctt caacttgatc tgcttctgct tgtaaagcat tcgcatcctc gctgaacaac
     1321 ttcacttcaa ttggttcggg attaccctgg agatcaccta tcacatcggg aagaatgcca
     1381 acgaattcaa catccaggga tggttctgac tcggcaatct gctcgcgcag gtcagtgatg
     1441 acgtccgcgg tgctgcgttt atgaccgggc ttaagtttca cagcaaagtc gcctgtgttt
     1501 ggctcggtca caaaaagacc catttcaatc cccgttcgtc ttgagtaact ctcgatctcg
     1561 ggtgtcttca acaacatttc ttcgacgtgt tgaagcatgc ggtctgtttc agcaagagaa
     1621 gacccgggcg gcgcccgata gtcgagcaca aatgcgcttt catcgaactc aggcaggaat
     1681 tcggatccga gtagtccgta aatcgaataa gaaccggcga gcacaatcac gatcgtcgcg
     1741 actacgatcc acttgttgtc gagcgcgaag ctaagtaagg attcgtaacg cgaaatgatc
     1801 gctcccagga atcggccatg agtctcttcg ttcgtgcctt ctggtttagc ttttacgaat
     1861 cgttcagcaa ggaccggcgt aaaagacaag gcaaggacca gcgaggtcaa aagggccacc
     1921 gtcatggtca aggcgagcga tcgaaaaaat acccctgtga ctcccgtcag taacgttaag
     1981 ggaaggaaca caaccaccgg tgtaatggtc gagccaatga taggtattgt aatttctgat
     2041 acggcgcttt gtactgcctc gcgtcgggat tggcctctcc cgagatgcgt gtagatattt
     2101 tctaccacga caattgcgtc atcgataacc agaccaatcg cagccgcaac accgccgagg
     2161 gtcatcagat caaaactgag actagcgagc cacattgcca ggaacgtaac aagtactgtc
     2221 accggaatta caaggatcgc cacgaatgta gttccccagt tacgcaggaa ggcaaagagg
     2281 atcacgacgg acaacaacag tccgattaaa atcgaatctc gcacggagtt gatcgactca
     2341 cgaaccagta gagactgatc gtaataagcg gcaactcgta cgtcttttgg gagttgaccc
     2401 tgcattgacg acagtgctgc cttaacctca tcgactacag caacggtatt cgcatcaggc
     2461 tggcggttaa tattcacgag cgcggcagga tgtccgtctg ccgtgactat tgtgtattca
     2521 ggttcaacgc cagcggaaac cgtggccaca tcagcgacac gaactggcgc attgttaaca
     2581 gtggcgacga cgatattgtt gagttcatca gaccttttcg cctgtccgct aattaaggcg
     2641 agctcaagct ggtggttttc ctcaattaga ccgggcgaca caatgatgtt tgcaccgcgg
     2701 atggcttcaa ctacctgttg ggcagagacg ttgtgagcgg ttaagcggtc gggatcgatc
     2761 gtaacgtgaa actctctcgt tttaccgccc gccactccga ccaccgccac tccgggaagt
     2821 ctcgcgagtt gcggccgcac gatgtaattg gcgagatccc gcaacgttga cgcatcgcgt
     2881 ctatcggaag ttaagctgta gccggttacc ggaaataccg caaaagttag tcgttcaatg
     2941 tttcgaatct gggcgccttg cggcagcgtt ggaagcagtt gcgacattct ggcctggacc
     3001 agttgcagtt cctgaataat gttgccgttc caatcaaagt agaggttgat ttcagatgag
     3061 ccacgcgcag tttttgactt gattcttgcg attcccggaa tcccgttcat cgcctcctcg
     3121 atcgggcgag ttactgagac cagcgtttgc gacgccggag cctcaccgtt atcaactatg
     3181 attacaatgc gcggaaaatc ggtttgtgga aaaacagcga tcggaagttg ccaggcggca
     3241 taaagtcccg cggcacaaag taggccaacg agcactacaa ttgcgcgaga ttgatttgaa
     3301 actacgctgg cgattcccat ttgaatacta ttaccgacga agttgactca ttcctttttg
     3361 tctttctcct cgttgttctc tttctcgtca gtagctatct ccactttcgt tccatcgggc
     3421 aatgagtaat taccttcgac cacgactgtc tctccgccct ttaatccttc gacgatttcg
     3481 accttctcag ccgtgcgaat gcccgccgtt accttcgtct catgtgcaac gttttgagca
     3541 tcaacaacca tgacggttcc ttcgtcagca ttactggatt caagagttac ggcagacaaa
     3601 ggcacaacga tcgcatcgtc ctttgagttt gcgaacacgg tcacttgagc ggctccgttc
     3661 ggtcggagtt ttccgtcttg atttccgagt gttatccaga cttcgaccgt tcgactcgtg
     3721 ggatcggtcg agcgactaag tagcgtgatt tggcccttac gttcttccga ggatgagtct
     3781 gtaggaagaa ctgttgccgc atcgccggtc ttcagttgag cagccgcagt gtccgagaaa
     3841 ggcgctttta cgatcactga actcgagtca gcgatcgtta ctaactttcc tccggcggac
     3901 gcaaagtcac cttcgtattg gaactgatcg gtaacgattc cggtgatcgg cgatcggatt
     3961 actgaataac tgagttgcgc gtcgagcgtc gcaagtcgtt gttctgcttg agcggtcttt
     4021 gcagcggcga gtgcgcgatc atttggattg agcgatcgag ctcgcaaaga cacagtctgc
     4081 tcctgcaaac gtaattcgtc ctcggctgtt gtgagctcga gttgcgcggc ttccaagtcc
     4141 ttcttggaaa taccaccatt agcgaagagc gctcgacgac gctcatacgt cgctcgagtg
     4201 ttgtttaccc tggctcgtgc gtcgagtaga gccttttgat cttccgcgtt ggtcttgggg
     4261 atggtgccac tgacgaagct acgctcattt gcacgcgctt cattcagtgc tcctgcggcc
     4321 tcagcacgct gagcctgaag atctcgcgac tccaaaacgg caatgacttc accagctcgc
     4381 accactttat tcttgaggag cgccatctgt ttgatctgcg cgctgatttt tgcagcgacg
     4441 tcagcttttt ccctcggcca gatggttcct atcgcagaga tctcggcagc tatcggccct
     4501 ttttcagctt taattacttt aacgcccact gtaggcgtta tttcggcctc attctctttt
     4561 gaagattttc gccaacgcca gataagaaag gttaagagga caagtcccag aactgcgatg
     4621 gtaaccagga caatatttcg ctgacgtaca attcgtgccc gtcgagctct ttgaacttca
     4681 tcttcaaagg aggtagtaga agactctttt tcctggatgt cgttctggtc ctgactcata
     4741 tattcgcttt aatggccagt cgactgtttc agtcgggcca gtgcaacttg ataatcaaag
     4801 atggcttgat aaagagcgct acgttgcgcg accagtgttg tctgtgcgtc tgtaacttca
     4861 actatctggg cttcacctgc tcgataacgc gcgattgatg acgcaacatt gctctccgcc
     4921 ttaatcacac cttgacgcgc gatgtcgatc cgatttgcgg cgcctgatgt ttgagctcgt
     4981 gctgcataga actgctgggt aaaaccgcga agcgccatgg cacgctcgtt ttgtgcaaga
     5041 ttcgctcgca agcgcgcctg cgcctcttta ctacggctgg tcccccaatc aaaaattggt
     5101 atggttaaac tgatctgggc cgaaacgccg ctgtgttctt tcagtagagg atttcttaaa
     5161 gaatcagaat cgaagccgcc gttgaacgaa taagatagag acggcctgcg ttcagccctt
     5221 gcgaccttta tatcttgctg tgcagccagc aatagttgat caaactggat gaactccggc
     5281 cgatttgaaa tctcactcgc tttgaattgt cgatcctcgg cgcttgttgg aattgccagt
     5341 gatagatctg tggttgtaat tgggcgagtg aactcaaaac cgacgagcac tcgcaacgcc
     5401 ccggctgctg cctcttcgtt ggtacgtgcc cgttccaatt catcgcggcg tgaaagcgtt
     5461 tgcagttgcg ctcgtgtgag atcaaccgag gcaacttctc ctccgcttag caagagcgag
     5521 gttatgtgct caaactcctc cgcagaggtg aggctccctt cagcagcttg acgttgagcc
     5581 gatgcgagcg ccaggccgta ataaccttcg atcactgcct gggccaatgc gcgcctggct
     5641 acctcggttc ccgcatgcgc tgcggcgagt aatgctctgt tcctcgctac cgtggcccga
     5701 agtcttccgg cgatatcgaa atctcccgaa acacttagga atgccacgta ctcattgatc
     5761 gcgttattcg cgataaagct gaatgtccgc ggctcgccag gtggtaagcc gagtgacggt
     5821 ga
//
LOCUS       DASSFP010000443         4929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_281348, whole genome shotgun sequence.
ACCESSION   DASSFP010000443 DASSFP010000000
VERSION     DASSFP010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281348"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VFH91_09965"
     CDS             <1..1045
                     /locus_tag="VFH91_09965"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET6863366.1"
                     /translation="GPPTALDFYMPAEWVTHSATWLTWPKDPETWPDRVEQVQQIFLQ
                     MLAALTPHETVNLLVDDEETQKYVLSQCTFESTSNIIFHQIETVDSWMRDYGPNFLVN
                     KSRDIAFNNWQFNAWGNKYEALKKDDRIPEIIAEVLQLQYFDPGIVLEGGSIEVNGAG
                     YLLTTEQCLLNPNRNPALTQKEIEGYLNAYLGVQKILWLGEGIVGDDTDGHIDDIARF
                     VNQNTIVCAVEDDPEDANYHLLQDNLKRLAAMTDVAGNEFKIVTLPMPGVVGGSSTDS
                     RNLDRLPASYANFYIANNVVLAPVFGHANDALATSTLQRVFPDRQVIPINCEPLVWGM
                     GTIHCVTQQQPLP"
     gene            complement(1042..2442)
                     /locus_tag="VFH91_09970"
     CDS             complement(1042..2442)
                     /locus_tag="VFH91_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6863367.1"
                     /translation="MEQIPEKVIALASAIRDCGGRALLVGGCVRDQLMDLKPFDWDLE
                     IYGVEPANVRTVLDQFGPVNVVGESFTVYKLGHDLDVSLPRRERKVGRGHKAFVVEGD
                     PWMSVADATRRRDFTINAVLMDPLTKEIIDPFYGSRDIENRVLRAVAIDTFPEDSLRV
                     LRAAQFASRFQFNVEPQTVELCRSIELDDLPSERIWGEIEKLLLLSSMPSIGLKWLRE
                     LNVIEKLFPEINRLIGVTQDPVWHPEGDVFEHTLLVVDVARQLIDGLSYAKKVTVMLA
                     ALAHDFGKPATTKFQDGHWRSLGHAEAGVGATERFLDRLNINTLENYNVRHQVIALVK
                     DHLKPGEFFKKRDEVGEGAFRRLARRCEPELLYRVAKADSLGRNADWVPREKWFDSKA
                     QEWFIERARELQVDLGPPEPILLGRHLLEMGLKPGVRIGEITSAIYEMQLDGRVRSIE
                     EAKSAARTLIGEEIAD"
     gene            complement(2519..2878)
                     /locus_tag="VFH91_09975"
     CDS             complement(2519..2878)
                     /locus_tag="VFH91_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863368.1"
                     /translation="MPVVKKRNQQTETRLLLESGEPVELPPAEIHESAVLVQFEKFKP
                     FINELLEVLRAGTIAEPVPVMLDGSTFAIHGPTRTFWVKLWHAAGSDRTKIDRAQILS
                     CLPSTRGVQILVEPIEE"
     gene            complement(2882..4333)
                     /locus_tag="VFH91_09980"
     CDS             complement(2882..4333)
                     /locus_tag="VFH91_09980"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET6863369.1"
                     /translation="MPAPNIDTIVSLAKRRGFVFPSSEVYGGLGSAWDYGPLGVEICN
                     NVKQSWWRWLVYDRDDIEGIDSSIILNRLVWQYSGHEETFNDPLSDCRECKSRLRADK
                     LPLPEGAAVGADVGQLIREKKIKCPNCGSSDLTEPRLFNMMLRTTLGAAAEENDPTAL
                     AYLRPETAQGIFINFLNVQTTMRRKLPFGIAQVGKSFRNEITPGNFILRTREFEQMEM
                     EYFVKPDAEHLALVDEWAEQYVEWFKSLGISEENLHLHEVPGEELAHYSKRTVDIMYR
                     YFPERAEEERQWEELMGIAYRTDFDLRSHSSKAVDEEGNRINPDSTEDLSYFDESTKQ
                     RFYPHVIEPAAGVNRTVLAVLMDAYSERTNDRGETRVILKLSPQLAPIKAAILPLAKN
                     KPEIVGMAKAIKRSLQPVLRAVYDDTGGIGKLYARQDEIGTPFCVTVDHQSLEDEAVT
                     VRDRDTWEQDRIKVTDLAEHLQKRLKPFPVTVD"
     gene            complement(4455..4616)
                     /locus_tag="VFH91_09985"
     CDS             complement(4455..4616)
                     /locus_tag="VFH91_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863370.1"
                     /translation="MADEGGGSGSTGVVAILAIFVLVILIALFMFRGRIFGGGGTQKI
                     DVNIQTPSK"
BASE COUNT         1232 a         1323 c         1141 g         1233 t
ORIGIN      
        1 tggaccacca actgctcttg atttctacat gcccgccgag tgggtaactc attcagctac
       61 ctggctaacg tggcccaaag atcctgaaac atggcccgat cgcgtcgaac aggttcagca
      121 gatatttctt cagatgctcg cggccttgac tcctcacgaa actgtgaatc tgctcgtaga
      181 cgatgaggag acccaaaagt atgtcctgag tcagtgtacc tttgagagca cgtcaaacat
      241 catcttccat caaatcgaga ctgttgactc atggatgcgc gattatggac caaactttct
      301 cgtcaataag tcgcgtgaca tcgctttcaa taattggcag ttcaacgcgt ggggtaacaa
      361 atacgaagcg cttaaaaaag atgatcgaat tcctgagatt atcgcggaag tcttacagct
      421 gcagtatttc gatccgggga ttgtcttaga gggcggatcg atcgaagtca atggggcagg
      481 ctatttactc actaccgaac aatgcctact aaatcccaac cgtaaccccg cattaaccca
      541 gaaggagatc gagggatatt taaacgccta ccttggagtt cagaagattc tttggcttgg
      601 agaaggcatc gtcggtgatg acaccgatgg acacatcgac gacattgcgc ggtttgtaaa
      661 tcaaaacaca attgtctgcg ctgtcgaaga cgacccagag gatgcgaact atcatttgct
      721 gcaagacaac cttaagagac tcgcggcaat gactgacgtc gctggaaacg aattcaaaat
      781 cgtgacgtta ccaatgcccg gtgttgtggg tggctcgagt actgactcaa ggaacctcga
      841 tcggctaccg gcgagttatg ccaattttta tattgccaac aacgtagtct tagctcccgt
      901 ctttggtcac gctaatgacg cgttagccac atctactctt caacgtgtct ttccggatcg
      961 gcaggtgatc ccaatcaatt gcgaaccctt agtgtgggga atgggaacaa ttcactgcgt
     1021 cactcaacaa cagccgctac cttaatcggc gatttcctcg cctattagtg ttctcgccgc
     1081 gcttttagct tcctcaatag atctcactcg accgtcgagt tgcatctcgt aaatggcaga
     1141 agtgatctcg ccaattcgaa ccccgggttt tagacccatc tccaataggt gtcgtcccaa
     1201 aagaattggt tcaggcggtc ccaaatcgac ttgaagttct ctggctcgct caatgaacca
     1261 ttcctgagcc tttgaatcga accatttctc gcgaggcacc cagtcggcat ttcgaccgag
     1321 tgaatcagcc tttgccaccc gatataacaa ctcgggttca caccttcgcg ccaatcgtcg
     1381 aaaggcgcct tcaccgactt cgtccctttt cttgaaaaac tcacctggtt tgaggtggtc
     1441 cttcaccagc gcaattacct ggtgacgaac gttgtaattt tcgagcgtat tgatattcaa
     1501 acgatccaga aatcgttctg tcgcgcccac tcctgcttcg gcatgtccga gagaacgcca
     1561 atggccatcc tgaaatttcg ttgtcgccgg ttttccaaag tcatgcgcta aagctgccaa
     1621 cataacagtc acctttttgg cgtacgacaa cccatcaatc agttgtctgg cgacatccac
     1681 gaccagaaga gtgtgctcaa acacgtctcc ttcgggatgc cacacaggat cttgtgttac
     1741 cccaatcaag cggttgattt cagggaagag cttttcgatc acatttaact cgcgtaacca
     1801 cttcaaaccg atagacggca tactcgagag cagcagaagc ttctcgatct ccccccagat
     1861 tctttcggat ggcagatcgt caagctcaat cgagcggcaa agttcgacag tttgtggttc
     1921 gacgttaaat tggaaacgac tcgcgaattg agcggccctt aaaacacgca agctgtcctc
     1981 tggaaaggtg tcgattgcaa cagcccgtag cacacggttc tcgatgtctc gactgccgta
     2041 aaagggatcg ataatctcct tcgttagtgg atccatcaag accgcattga tggtaaagtc
     2101 gcgacgcctt gtggcatcag caacactcat ccagggatcc ccttcaacca caaatgcttt
     2161 atgaccgcga ccaacttttc gttcacggcg tggcagcgat acatccaggt cgtgacccag
     2221 tttgtagact gtaaaagatt cgcctacaac gttgactggg ccaaattgat cgaggaccgt
     2281 acgcacatta gcgggttcaa ctccatagat ttccagatcc caatcaaagg gcttaaggtc
     2341 catcaactga tcgcggacac aacctcctac gagcaaagcc cggcccccgc aatctcggat
     2401 tgcggacgct aaggcaataa ctttttccgg aatttgttcc acaacgctac tctccaacct
     2461 ctgattttca gagtcaatag ttgcgggcta aaggcgggat acgaacgcca actaaggtct
     2521 attcctcaat gggttcgact aagatctgaa caccgcgagt agaaggcaaa caactgagaa
     2581 tctgagcacg atcgattttt gtccgatcgc tcccggcagc gtgccagagt ttgacccaaa
     2641 aagtccgagt tggaccatga atggcaaaag tcgacccatc cagcatcacc gggacgggct
     2701 cagcaatcgt ccccgctcgc aatacctcca gtaactcgtt tatgaacggc ttaaatttct
     2761 cgaattgaac caggactgct gactcgtgga tctccgcagg cggcaactca actggttctc
     2821 cgctctcgag cagcaaacgc gtttccgtct gttggtttcg cttcttgacg acaggcataa
     2881 ctcaatccac cgtaacagga aatggcttca aacgtttctg taagtgttca gccaggtccg
     2941 tcactttaat tcggtcctgt tcccatgtat cacggtctcg cactgttact gcttcatcct
     3001 ctaaagactg atggtccaca gttacgcaga aaggcgttcc aatctcgtcc tgtcgagcat
     3061 atagtttgcc aatccctccg gtatcgtcgt acacagctct cagcaccggc tgaagagatc
     3121 tcttaatggc ttttgccatg cccactattt caggtttgtt cttggcgagc ggcaggatcg
     3181 ccgccttgat cggagccaac tgagggctca acttcaggat gactctggtt tctcctctgt
     3241 cattcgtgcg ctctgaatag gcatccatta aaaccgcgag gaccgttcta tttacccctg
     3301 cggcaggttc gatcacgtgt ggataaaagc gttgtttcgt ggattcatcg aagtaagata
     3361 aatcctctgt agagtctgga ttaatacgat taccttcttc atcaacggct ttgctcgaat
     3421 gggatctcaa gtcgaagtcg gtccggtatg cgattcccat cagctcttcc cattgacgct
     3481 cttcctctgc ccgctcgggg aaatacctgt acatgatgtc gaccgtgcgt ttcgagtagt
     3541 gggccagttc ttcgcctggt acttcgtgca agtggagatt ctcctctgaa atgcccagac
     3601 tcttaaacca ttcaacgtat tgttcggccc attcatcaac aagagcgaga tgttcggcat
     3661 ccggtttaac aaaatactcc atctccattt gttcaaactc acgggtccgc aaaatgaagt
     3721 ttcccggtgt gatctcatta cgaaatgact tacctacctg ggcgatccca aacggcagct
     3781 tccgccgcat cgtagtttgc acgttcaaaa agttgataaa gattccctgg gccgtctctg
     3841 gtctaagata tgccagtgcc gttgggtcgt tctcttcagc tgccgctccg agcgtagttc
     3901 gcagcatcat gttgaacagc cgcggctcgg ttaaatcact cgaaccacag ttcggacact
     3961 taatcttctt ctcgcgaatc agttgaccaa catcagcgcc taccgcggca ccctctggca
     4021 gcggcaattt atccgcgcgc aagcgactct tacattcgcg gcagtcggac aaaggatcgt
     4081 taaaggtttc ctcatgtcct gaatattgcc ataccaatcg gttaagaatg atcgatgagt
     4141 caattccttc aatgtcatcg cggtcgtaga ccaaccatcg ccaccatgac tgcttgacgt
     4201 tgttgcagat ctcaacaccc agcggaccgt agtcccaggc gcttccgagt ccgccatata
     4261 cttctgacga aggaaacaca aaaccccttc gttttgcgag agagacgatg gtgtcaatgt
     4321 ttggcgcagg caattcgatc gctatcctta attggaattg aatcgtgaaa tagtagaaga
     4381 cacatcctga aaagaaaaga agagaggaca gttaactgct gtcctccctc gtttaactgc
     4441 tcgctgacct gagactattt actgggtgtc tgtatgttga cgtcgatctt ctgagtaccg
     4501 ccacctccaa agattcgacc gcggaacatg aacaaagcga tcaggatcac taatacgaag
     4561 atcgctaaaa tcgcaacgac accagtacta ccactaccac cgccctcatc agccatgact
     4621 aaaccctcct ggtcggaatg tgatgttagg gggctaacag ggaacggtag atacgaattg
     4681 ttaacacacc gaacgaaggg tcgtcaaata agtccaaaca taagtactgg ttcagtttga
     4741 attctttctc agtctcatag tggctcggag ccggttggca ttaaggtcaa ttcgccgcag
     4801 ggttacaccg aaggtgttcg ccaatttcag cccagggttg agctttggca accctgggga
     4861 taaaggcggg tgattgtttt gtcgcaaacc tgaagggatt gcgacgggtt ttgcgcgcgg
     4921 cgggcgcaa
//
LOCUS       DASSFP010000444        10089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1461389, whole genome shotgun sequence.
ACCESSION   DASSFP010000444 DASSFP010000000
VERSION     DASSFP010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461389"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VFH91_09990"
     CDS             <1..1906
                     /locus_tag="VFH91_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863371.1"
                     /translation="TPHRQHPWWQVMCLTGVDYFSTLGYQPGIAFLAAGALSPIATLI
                     LVLLTLFGALPIYNRVAEESPHGEGSISMLEHQLSRWKGKLFVLVLLGFVATDFIITI
                     TLSAADATAHIIENPFTPELLKHQIGVTLTLIAFLGAIFLRGFKEAIGIAVTLVGVYL
                     FLNVIVVAFGLYQLSTHAYHFPEWKAALLHHPSVHGDVWLIVFFSLILFPKLALGLSG
                     FETGVAVMPLVKGDVELTEGDWESIHSTRTGDRKQTKAQQLLQGRIINTKKLLRSAAL
                     IMSVMLISSSLVTTILIPPEKFAEGGEANGRAIAYLAHHFFGDIFGTVYDLSTIMILW
                     FAGASAMAGLLSIVPRYLPRYGMAPEWARAARPLVIVITVIAFAVTLIFNADVDQQGG
                     AYATGVLVLMTSAAIAVTLAVRRKQGNWYPYLLIALVFLYTTIQNIHERPEGIKIASF
                     FILTIIVASLVSRVWRSTELRVEQIELDDQARQFIKDAAKGTIRIITNRRDKGDAPEY
                     ELKENEKRTNNHIPAGEPILFFEVTPGDASEFSGTLKIRGESINGYRILRTESAAVPN
                     AIAAFLLYLRDEMGKLPHVYFGWSEGNPISYLLKYIAFGEGDTAPVTHEVLRKAEKDP
                     TRRPIVHVGG"
     regulatory      1924..2092
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2172..4163
                     /locus_tag="VFH91_09995"
     CDS             2172..4163
                     /locus_tag="VFH91_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6863372.1"
                     /translation="MIANLKRMLIGSALRTEQASHERLTKKTALAVFSSDALSSVAYS
                     TEAVLLVLLAAGSIAISYLPMIAIGITMLLAILVISYRQTIHAYPSGGGAYIVAKDNL
                     GTLAGLIAAASLLVDYILTVAVSVTAGVAAITSAAQGTRFAPVQEYRVLLCILFVIFI
                     SVVNLRGVRESGSFFAGPTYLFIFSMLALIIVGFIRFSTTHEVMQTPATLHFDESVGS
                     VAHHALTSAGLVWLLMRAFAAGCTALTGVEAISNGIPAFKAPESKNAATTLSVMAVVM
                     AVLILGSGFLAYALNAHPTPNETLVSAIARHTFGGGIFYYVLQASTAAILILAANTSF
                     ADFPRLASLLAADRYLPRQLATRGDRLVFSNGILILALFACLLIVVFKAEEQAMLPLY
                     ALGVFLSFTLSQAGMVVHWQRLRKADDAKRRIKIEEQIHRTQAEDTGAVSHEAERLEA
                     IERDRGGSWLGYMLINAIGAGVSFIVLLIIAVTKFSHGAWAVIVLIPLLVALFRAING
                     HYFDIAAQLSTEGLVPSSTVARHVVLVPISGIHRGVIYALEYAKSIAPGHVTAVYVNF
                     NEEATQKLKEKWLTWGEGVELVVLQSPYRSLTRPLLRYIERVSRQSDNLMVTVLLPEF
                     VPAKWWHQLLHNQSSLLLKGALLFRKGVIVTSVPYHLEK"
     gene            complement(4180..4554)
                     /locus_tag="VFH91_10000"
     CDS             complement(4180..4554)
                     /locus_tag="VFH91_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HET6863373.1"
                     /translation="MAQTELAHEIARSPESEYQDLPIKCVDCNENFIWTAGEQVFFHD
                     KGLKNEPKRCKPCKQAKNERLAAIAAAQSAGVRQRIEVSVQCAQCGQQTTVPFYPSQG
                     RPVYCRSCFLAGRTSSTEAPTT"
     gene            4801..6099
                     /gene="purD"
                     /locus_tag="VFH91_10005"
     CDS             4801..6099
                     /gene="purD"
                     /locus_tag="VFH91_10005"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET6863374.1"
                     /translation="MKILVLGSGGREHAIVWALNKTASETPTIFCAPGNPGISQIAKS
                     LPFSLEAQDDLVAFAKSERIDLTFVGPEAPLAAGIVDKFQSVGLPIVGPTSSAARLES
                     SKVFAKNFMARFAIPTASFRVAENPSEALEILRRGDVSSGSGRVVLKADGLAAGKGVI
                     IADSLPEAEKGVNELMVTAVAGKEAGARLVIEEPLSGQEASLLLFADGRDYAVMPAAR
                     DHKRIGEGDAGPNTGGMGCITDDSILSPDLLQRVLRQIVEPTLEGARAEGSEFKGVLF
                     LGLMLTPEGPKLLEYNVRFGDPETQAILVRLRTDLAEIFEAIRAGYLGRLKVEWSKGS
                     SACVVLANRGYPGKYEKGATIEGLSALQGDPVTVFHAGTTQGAGGQILATGGRVLGVT
                     SDGQDLPQALRRCYGAIEKIHWEGMQFRRDIGRSTLTQIE"
     gene            6281..7051
                     /locus_tag="VFH91_10010"
     CDS             6281..7051
                     /locus_tag="VFH91_10010"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET6863375.1"
                     /translation="MGVWTRMKRSIRALFGGIIEKTEDPELILQQTIRDMRDRVPELN
                     NSVAQVMATEKLLAKNKERLESQVVDLDSKIRASVKMGRDDIATAYIGQLQQAQVDLQ
                     KTGQQLEHANLASKQALKARDNYVLQMQRRTAEAMQLINQSKQAKLQEQLAQTMESFQ
                     LGDDASTFNEMRDKIDRRAAAAEAKMQLGSASVDTQMQEIEREALDMQLQDKLLAYKR
                     DMGLLGTGSGAPTPQALPAEGETHSGGSNGTGNGSRSG"
     gene            7078..7947
                     /locus_tag="VFH91_10015"
     CDS             7078..7947
                     /locus_tag="VFH91_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863376.1"
                     /translation="MSTSSSINKFMAEFPKFDIRYLKEAFKEPINFWGMAGFAVAAAY
                     VQDVTPLVAALAAETLYLATVPASTVYRRLVDRREKQRLLKLREQQREALIKQFDPRE
                     REAVEYLRWMKNQIYSNYKKFTGTKQVPHNIQSLDQRWEDFVDLLDVYRRRKHHLRSI
                     NRQAVQNQLVQAERSVQVAKDDRERRIQAANVEILKRRVAAFNDIERSVKLVEGQLQS
                     IENFFGLVNDQVVTLPTPERVSSLDFEQLSDSIAMTKQMLEETSDTFAALDSHNRDIG
                     NYELLLSNTGTSK"
     gene            8356..>10089
                     /locus_tag="VFH91_10020"
     CDS             8356..>10089
                     /locus_tag="VFH91_10020"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HET6863377.1"
                     /translation="MSAAIGKNVGNKVTTPAAPPRQKLQQLGLNAKKVVSKRYSLKDS
                     KGRAIEEWADIVRRVVGHVSAAETGEKRDKFFAAMSQVMLEREFIPNTPCLVNAGKAN
                     GQLAACFVLEVPDSLTGIMDHAKAAATIHQTGGGTGMTYEFLRPSGAMVSSATGVASG
                     PVSFMNIVNQVTDVVKQGGVRRGANMGMMRVTHPDVLRFIHAKNDQHSLTNFNISVNV
                     TDKFLKAVDDNEWFQLEFNGESWKDSIYDPVRDGDYVIYRRPDGSTVTFCDRQSFLST
                     DLSNTTIEEPPLPGMIFAPDIWNRIVASAHKYAEPGIAFIDEVNRHNHMMKSMGPIYS
                     CNPCGEQFLHFSNSCNLGSIDLAKFYDPEMRIDWDRLREVTHLCTRFLDNVIDTCAWP
                     LPEINDVVHRTRPVGLGIMGFADLCLNLNVTYGSAASIDLMDEVMGFVRREAWIESNR
                     LGAEKGTFPEFEPNREAYTEFLTNQIGIPKDVPLTPRNYEVTTIAPTGTISLVAETSS
                     GIEPNFSWAYVRKDTLGTRTYVHTLAARALGIDVDQSDQQSIDAAAAYVVEHESELPP
                     HFISAMNISAEQ"
BASE COUNT         2465 a         2445 c         2649 g         2530 t
ORIGIN      
        1 gacaccccac agacaacatc cctggtggca ggtgatgtgt ttgaccggcg tcgactactt
       61 ctcaactctc ggttatcaac ctggcattgc attcctcgca gccggcgcac tctcaccaat
      121 tgctaccctg atactagtgc tgctaactct cttcggggcc ctgccaatct acaaccgggt
      181 cgcagaagag agtccgcacg gcgaaggttc tatctcaatg ttggagcacc agctttctcg
      241 ttggaaggga aagctatttg tgctggttct ccttggattt gtcgcgaccg acttcatcat
      301 aaccattacc ttgtctgccg cagacgcaac ggcccacatc atcgaaaacc cgttcacgcc
      361 cgagcttctg aagcatcaga ttggagtcac ccttacgctg atcgcattcc tgggagctat
      421 ctttctcagg ggctttaaag aagctattgg aatcgcagtc acattggttg gagtctactt
      481 gttcttaaat gtgatagtag ttgctttcgg cctctatcaa ctttcgactc atgcttatca
      541 tttccctgaa tggaaagcgg ctttgttgca tcatccaagc gtacacggtg acgtctggtt
      601 gatcgtcttc ttttctctga tcctttttcc gaaactcgct cttggtctat ccggattcga
      661 aacgggagtt gccgttatgc ctctcgtaaa gggtgacgtt gagttgacgg agggtgattg
      721 ggaaagcatt cattccacgc gaacaggtga ccgtaagcag accaaggctc aacagctgct
      781 tcaaggtcgg ataattaaca ccaaaaaact ccttcgttca gcggcgctaa tcatgagcgt
      841 gatgctgatt agcagtagct tggtcacgac tattcttatt ccccccgaaa agtttgccga
      901 gggaggggaa gcaaatggca gagcaattgc gtacttggcc caccattttt tcggcgacat
      961 cttcggaacg gtctatgacc ttagcaccat catgatcctc tggtttgccg gagcctcggc
     1021 gatggccgga ctcttgagta tcgttccgcg ttatctgcct cgttatggta tggctcctga
     1081 atgggccaga gctgccagac cgttagtgat cgtaatcaca gtgatcgctt tcgcagttac
     1141 actgattttc aatgctgatg tcgaccaaca aggcggagcg tacgctactg gcgtgttggt
     1201 cctaatgacg tctgctgcca ttgctgtgac tttggcagtt cggagaaaac aaggaaattg
     1261 gtatccatat cttctgatag cgctggtgtt tttgtacacg actattcaaa acattcacga
     1321 gcgcccggaa ggtatcaaaa tcgcttcttt cttcatccta acaatcatcg tggcttcatt
     1381 ggtttccaga gtctggcgtt caacggaact tcgagtagaa cagatcgagc tggacgatca
     1441 agcacgtcaa tttataaaag atgcggcaaa gggcacgatc agaatcatca ccaatcgtcg
     1501 cgacaaaggt gacgcgcctg agtacgagct aaaagagaat gagaagcgca cgaataacca
     1561 tatacccgca ggcgagccga ttctgttttt cgaggttacg ccgggtgatg cttctgagtt
     1621 ttcgggaaca ctgaaaataa gaggcgagtc gatcaatggt tatcgcattc ttcgaaccga
     1681 atcagcagca gtgccgaacg cgattgcagc gttccttctt tacctgcgtg atgagatggg
     1741 taaacttccg cacgtctatt ttggttggag tgaaggtaac ccaatctcgt atctcttgaa
     1801 gtacatcgct tttggtgaag gcgataccgc gcccgtaaca catgaagttc tgcgcaaagc
     1861 ggagaaagat ccgactcgac gacctatcgt tcacgtaggc ggataagtct tgatggtatt
     1921 tgacagcaga gtttgccgaa gtgtcgtggc tgtttgaaac gacacgcggg ggaaccacag
     1981 tctcacctgg agacaggggc gaagcgtgaa tcaaccgttg gttgaaaatc atgtccgggt
     2041 acttccaacc cgagcccgtc agctaacctc gtaggcgtta aaggaagcaa tgccccgcga
     2101 tttcgctgaa cgccgagtta attgctcggc gttatctatt tattcggggc ctgacactct
     2161 cggaggttgc cgtgattgcg aatttgaaac gcatgctcat tggaagtgca ttgcgcactg
     2221 agcaggcaag tcatgaacgt cttaccaaga aaaccgcatt agccgtcttt tcttcagatg
     2281 ctctgtcgtc agtagcctat tcaactgagg ccgttcttct ggtcctatta gccgccggta
     2341 gtattgctat ctcgtaccta ccgatgatcg ctattggcat aacgatgttg ttagccattc
     2401 tggtcatttc ataccggcaa acgatacatg cgtatccctc aggcggcggt gcttacatag
     2461 ttgcgaagga caatcttgga actcttgcgg gattgattgc cgcagcatcg ttgctggttg
     2521 attacattct gactgtcgcg gtctctgtta cggctggagt cgctgcaatc acttccgctg
     2581 cgcagggcac tcgcttcgcc ccagtgcaag aatacagagt gttactttgc attctgttcg
     2641 tgatctttat ttcggttgtg aatcttcgtg gtgtgcgtga gtcagggtcg ttctttgctg
     2701 gtccaactta cctttttatc ttctcgatgt tggcgttgat tattgttggc ttcattcgat
     2761 tctcgaccac acatgaggtc atgcaaactc cggccacgct gcattttgac gaatcagtcg
     2821 gatcagtcgc ccaccacgct ttaacatcag ctggtctggt gtggttgctg atgcgtgcat
     2881 tcgcggccgg ctgcaccgct ttgaccgggg tagaggcaat ctcaaacgga atcccggcat
     2941 tcaaagcgcc cgaatcaaaa aacgctgcaa cgactctgag tgtgatggct gtagtcatgg
     3001 ccgtattgat ccttggttca ggatttcttg cttatgcact gaatgctcat ccgacgccca
     3061 acgagactct agtctccgcg atagcgcgtc acacctttgg cggcggaatt ttctattacg
     3121 tgttgcaggc ttcaaccgcg gcaattctta tcctggccgc taacacctct ttcgctgatt
     3181 ttcccaggct tgcaagtttg ctcgctgccg atcgttacct gcctcgacag ctggcgacga
     3241 gaggagacag gcttgttttc tcaaatggaa ttctgatcct tgcattgttt gcctgtcttt
     3301 tgattgtagt gtttaaggcc gaagagcagg cgatgcttcc gctatacgct cttggcgtgt
     3361 tcctctcgtt cactttgtct caagccggaa tggtggtcca ttggcaacgt ttacgaaaag
     3421 ccgacgatgc aaagcgcaga attaaaatcg aagaacaaat acatcgaact caagccgagg
     3481 atacaggcgc tgtaagtcat gaggcggaac gtttggaagc cattgagcga gaccgaggtg
     3541 gcagctggct cggctatatg ttgataaatg cgattggagc gggggtcagt tttatagtcc
     3601 tactgataat cgcggtcact aaattcagtc atggagcatg ggcggtaatt gttttgattc
     3661 ccttgctggt tgcgctgttt cgtgcgatca acggtcatta cttcgacatt gccgctcaac
     3721 tgtcaacaga aggtttagta ccatcaagta cggtcgccag gcacgttgta ctagttccta
     3781 tttccggaat tcatcgaggt gttatctacg cgctcgagta tgcaaagtcg atcgcgcccg
     3841 gacacgttac tgctgtgtat gtaaatttta atgaggaggc gactcagaaa ctcaaggaga
     3901 agtggttgac gtggggcgaa ggtgttgagc ttgtggtttt gcaatcgcct tatcggtcct
     3961 taaccagacc actgctaaga tatattgagc gagtctcaag acagtcggac aatctgatgg
     4021 tcacagttct attgcccgaa ttcgttccgg caaagtggtg gcatcaactg ctccacaacc
     4081 agagttctct acttttgaaa ggggcgttgt tatttcgaaa aggcgtgata gtaacaagtg
     4141 tgccctacca cttagagaag tgatgactga agcgagttgt tatgttgtgg gtgcttccgt
     4201 actcgacgtt cgtcctgcta gaaagcaaga gcgacaatag acgggtcgtc cctgcgatgg
     4261 ataaaacggt acggtggttt gttgtccaca ctgcgcacat tgtactgaga cttcgatgcg
     4321 ctgccgcact ccagccgatt gtgctgcggc aattgccgct aatcgttcgt tcttcgcttg
     4381 tttgcaaggc ttgcagcgct tgggttcgtt tttcaaacct ttatcatgaa aaaaaacttg
     4441 ttcgcctgct gtccaaataa agttctcgtt acaatctacg cacttgatcg gtagatcctg
     4501 atattcactt tctggacttc gtgctatttc atgcgccagt tccgtctgtg ccatgatgaa
     4561 ctccctgggg tcgttaaggt taagaggttt ccaattgcaa taatgccggc ccgctgtaaa
     4621 acatcaatat ccagaatgga gttagctttt aggaatgggc agatcccttc aactccgaag
     4681 gggaccaact tggtcggcaa cgttataact atgtcgaaaa tgtgtcaaga caaaaacagc
     4741 tctttcgact tgtcaagttt ttgagttcta tcttggacca gtctgaacat cgtcaaaaaa
     4801 atgaaaatcc ttgtacttgg ctctggtgga cgcgagcacg caatcgtttg ggctctcaac
     4861 aaaacggcct ccgaaacacc aactattttt tgtgcgccag ggaacccagg catctcgcag
     4921 attgccaaaa gtcttccctt ttctcttgag gctcaagacg acctcgtagc gttcgcaaag
     4981 tcagaaagaa tcgacctgac tttcgtgggt cctgaagccc cgctcgccgc aggaatcgtc
     5041 gacaagtttc aatcggttgg actacctata gtcggaccca caagttctgc cgcccggctc
     5101 gagtccagta aagtttttgc caagaacttt atggctcggt ttgcgatacc gaccgcgtcg
     5161 tttcgcgtcg cggagaatcc gtccgaggcg ctggaaatcc ttcgtcgtgg agacgtctct
     5221 tctggcagcg ggcgcgtcgt cttaaaggcc gacgggcttg cagctggtaa gggcgtaata
     5281 atcgcagact cgttgcccga agccgaaaaa ggtgtgaacg aactgatggt tacggccgtc
     5341 gctggtaaag aggccggagc gaggttggtg atcgaagagc cgttaagcgg gcaggaagcc
     5401 tcactgcttc tctttgctga tggacgagac tatgcggtaa tgcccgcggc tcgagaccac
     5461 aaacgcatcg gagaaggcga tgctggccca aacactggtg gcatggggtg catcaccgat
     5521 gactcgattc tatccccgga tctgttgcag cgagttctga gacaaattgt cgaaccgacg
     5581 cttgaaggcg cgcgcgctga aggcagcgaa ttcaagggag tgctgttttt ggggctcatg
     5641 ttgacgccag aaggtcccaa gcttctggag tacaacgtca gatttggaga tcctgagact
     5701 caggcaatcc tggtgagact tcgcacagat ctagccgaga tttttgaggc tattcgcgca
     5761 ggctacttag gtcggctaaa ggtcgaatgg tctaaaggtt cctccgcctg tgtggtattg
     5821 gcgaatcgcg gctatccggg taaatatgaa aagggcgcca cgattgaggg cctttctgct
     5881 cttcaaggcg atccagtcac tgttttccac gccggaacta cacaaggcgc tggtgggcag
     5941 attctggcga cgggcggacg ggttttaggc gtgacctcag atgggcagga cctgcctcaa
     6001 gcgctccgcc ggtgttatgg cgccattgaa aagatccatt gggaaggaat gcagttcagg
     6061 cgtgatatag ggaggtcaac tttgacccag attgagtaat gcctgccgtg tgtggaaggc
     6121 ttgtaagagt gctgctatac tccagacttg attggttttt tcggcttagg atgaaactgc
     6181 ctgagcatat gcgtcgtaca aatgaacgtc gcaaggtctt gagaattcgg aggccgtgca
     6241 ccgagagatt gaatatctcg tggttggagg atgagctgag atgggagttt ggacacgaat
     6301 gaagcgatcg atacgcgcac tcttcggagg cattatcgaa aagaccgaag atcctgaact
     6361 cattctgcaa caaacaattc gcgacatgcg cgatcgcgtt ccggaattga ataattctgt
     6421 cgcccaggta atggcgacgg aaaaattgtt agcaaagaat aaagaacggc tggagagcca
     6481 ggttgtcgat cttgactcga agattcgtgc ttcggtgaag atgggacgcg atgacattgc
     6541 caccgcgtac attggacaac tacaacaagc tcaagttgat ctgcaaaaga cgggacagca
     6601 acttgaacat gccaacctcg catcgaaaca ggcattaaaa gcacgagaca attatgtgct
     6661 tcagatgcag cgtcgcacgg cagaggcgat gcagttgata aaccaatcaa aacaggcaaa
     6721 gcttcaggag caactcgccc agacaatgga gtcgttccag cttggcgacg acgcatcgac
     6781 gtttaatgag atgcgcgaca agattgatcg acgagctgct gcggctgagg ccaagatgca
     6841 attgggatct gcctcggtcg acactcagat gcaggagata gagcgcgaag ctctcgacat
     6901 gcaattgcaa gacaagctgc ttgcatacaa aagggacatg ggattgctgg gcacgggcag
     6961 cggtgcccct actccgcagg cgcttccggc tgagggtgaa acgcacagcg gaggcagtaa
     7021 cggaacaggc aatgggagtc gctcgggatg agttttatcc cggagcggat ttaggaagtg
     7081 agtacaagtt caagtatcaa taagtttatg gccgagtttc ctaagtttga tatcaggtat
     7141 cttaaagagg cctttaaaga acccataaac ttttggggga tggcggggtt tgcggtggca
     7201 gctgcttacg tccaggacgt cacaccgctt gttgctgcgt tggcagccga aacgctttat
     7261 ttagctacag tgccggcaag tacagtctat cggcgcctgg ttgacaggcg agagaagcag
     7321 cggttgctta agcttcggga acagcaacgc gaagctttaa tcaaacagtt cgatccgcga
     7381 gaaagagaag cggtcgaata tcttcgttgg atgaagaatc agatttactc gaactacaag
     7441 aagtttaccg gcaccaaaca agtgccgcac aacattcaga gtctggacca acgttgggaa
     7501 gacttcgtgg accttctcga tgtttatcgt agacgcaaac atcatcttcg ttcgatcaac
     7561 cgacaagctg ttcagaatca actggtccag gctgaacggt ccgtccaggt cgcaaaagac
     7621 gacagagaga gaagaatcca agctgcgaac gttgagatcc tcaaacgtcg agtggccgcg
     7681 ttcaacgata ttgagcgttc ggtaaaactc gttgagggac agctacagtc cattgaaaac
     7741 tttttcggtc ttgtgaatga tcaagttgtt accctgccta caccagagcg tgtctcgtca
     7801 ctcgactttg agcaattaag cgattcaatt gcgatgacta agcaaatgct cgaggagact
     7861 tcggatacct ttgccgcatt ggatagccac aatcgagaca tcggaaacta cgaactgctg
     7921 ctttctaata ctggaacctc gaaataggtt ataggtccta tgagtcctaa aggtcctatt
     7981 gcctataggg cctataagac ccataggacc tattcccagc gctgcacccc tgaaacccac
     8041 gaccatacaa tagtttagtt gttaatgttg aggctgaagt aatacactct atagcattga
     8101 aaaaaaagat ggccacaaga tattgacact cgaggtccaa aggagtatat tccgctccgc
     8161 ttgagaacga agtgataact ggtgcagcgg ccgaccccct gaggtgctct ccagccgggt
     8221 ttgtcgcttc gaaaagagcc aaccggagta tcagcaaaac actctaacga tgaaggcccg
     8281 cgtagcaata cgcctggcat aaccgttttt gaaactcgat ttactctcag tttccccggg
     8341 agaaaggcag agaagatgag cgcagctatc ggcaaaaatg tcggaaataa ggtaactacc
     8401 cccgcggcac ctccgcgtca aaagttgcaa cagcttgggc tcaatgccaa aaaagtcgta
     8461 agtaagcgat attctcttaa agactctaaa ggacgagcaa ttgaggaatg ggccgatatt
     8521 gtgcgccggg ttgtgggcca cgtatcagcc gctgagactg gagaaaaacg cgataagttt
     8581 ttcgcggcaa tgagccaggt gatgttggag cgtgagttta ttccgaacac gccctgtctc
     8641 gtaaatgccg ggaaggcaaa cggtcaactt gccgcttgct ttgtactgga agtaccagac
     8701 tcgctgaccg ggatcatgga tcacgccaag gcagcggcca ctattcatca gaccggtggc
     8761 ggtaccggaa tgacgtacga gttcctgaga ccatccggtg caatggtgag ttcggctacc
     8821 ggagtcgcct caggacccgt ctcctttatg aacatcgtca atcaggtgac cgatgttgtg
     8881 aagcagggag gcgtgaggcg tggcgcgaat atgggaatga tgcgcgtcac tcatcccgat
     8941 gtccttcgct ttattcatgc gaagaacgat cagcattcac ttaccaactt caatatttca
     9001 gtaaacgtta ctgacaagtt tctaaaagcc gtcgatgaca acgaatggtt tcagctggag
     9061 ttcaatggcg agtcatggaa agactccatc tacgatccag tacgagacgg cgactatgtg
     9121 atttatcgtc gacctgatgg ctcgaccgtc actttttgcg atcgccagtc ttttctgtcg
     9181 actgatctat ccaacacaac aattgaagaa ccgccattgc cgggaatgat cttcgcgccg
     9241 gatatttgga accgcatcgt cgccagcgcg cataagtatg ctgagccggg aatcgccttc
     9301 atcgatgagg tcaatcgtca caatcacatg atgaaatcga tggggccgat ttactcatgt
     9361 aatccttgcg gcgagcagtt cttacacttc tcaaattcct gcaaccttgg ctcgatcgat
     9421 ctcgcaaagt tctatgatcc tgaaatgcga atcgattggg atcggctgcg agaagtgact
     9481 catctgtgca cgcgcttttt ggacaatgtt atcgatactt gtgcgtggcc cctccctgag
     9541 atcaatgatg tggtccatcg aactcgtcca gtcggacttg gaataatggg cttcgcagat
     9601 ctttgcctca atctcaacgt cacttatggc tctgcggcgt ccatcgactt aatggacgag
     9661 gtgatgggtt ttgtacgccg ggaagcctgg attgagagta accggctcgg agctgaaaaa
     9721 ggaactttcc ctgaattcga accaaaccgt gaagcgtata cagaattttt aactaaccag
     9781 atcggtattc caaaggatgt ccccttaact ccgcgcaact atgaagtaac aacaattgcg
     9841 ccgaccggga cgatctcact cgtagctgag acttcgtctg gaattgagcc gaacttctct
     9901 tgggcctatg ttcgaaagga cacgctcgga acgcgtacct acgttcacac gctggctgca
     9961 agggctttgg gaatcgatgt ggaccagtct gatcagcaat caatcgatgc ggctgcggcc
    10021 tatgttgtgg aacacgaaag tgaactacca ccgcacttca tctcggcgat gaacataagc
    10081 gctgagcag
//
LOCUS       DASSFP010000445         3271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1322328, whole genome shotgun sequence.
ACCESSION   DASSFP010000445 DASSFP010000000
VERSION     DASSFP010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322328"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFH91_10025"
     CDS             <1..358
                     /locus_tag="VFH91_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863378.1"
                     /translation="RRMLVQENKWRAVRYGLEGKLIDFGKQIELPAGELVLELLEFVD
                     DVVDELGSRQEISHVHTILERGTSADRQLKVFRETNDLKAVVDDLMELTLENVPVSTD
                     VGISSPKTTTTSTISK"
     gene            complement(342..1019)
                     /locus_tag="VFH91_10030"
     CDS             complement(342..1019)
                     /locus_tag="VFH91_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863379.1"
                     /translation="MSRSQLLNDESLKEIALTLTRRDSDLARIHIHFGSPPLWQRRTG
                     FATLVHIILEQQVSLLSAKSMLNRLKAIVVPFTPERFCELGESYLRENGVTRQKASYI
                     IHLAKLIVTKQLNLAALSRMSDDDARRALMEVKGIGSWSADVYLLMAMRRPDIWPAGD
                     LALAIAIRDLKGLAEIPPPKVLEELAEGWRPHRAVAARMLWHFYLERKAAARNSVPRS
                     KAATSKS"
     gene            complement(1026..1943)
                     /locus_tag="VFH91_10035"
     CDS             complement(1026..1943)
                     /locus_tag="VFH91_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863380.1"
                     /translation="MTQELSATKSDNDLTEVTSTLPFPFNQQTFCRYEPIEQHIAKAR
                     VLVVDNLLRDEADISPVARREWGELLQSQLKRERVIAGMAIENIVNNISRLIKEPKTE
                     VAHVSEVARAASDFKPDAIVLSGTLRDFDYYEQSILDHFGDFIRKTDIPVLAICGGHQ
                     LVGLSFGARVITLDRLEQHEQREARTHEYQYRFVRITDPSDPIFTHIPLTDSDGSNVP
                     RILRVWQNHGLQLDRVPVGFKLLATSYLCRNQMMVKRSEGQLIYTVQFHLEKSFEDWN
                     KNRTRWEHPNDSRDGRILFENFLIEALKS"
     gene            complement(1952..2446)
                     /locus_tag="VFH91_10040"
     CDS             complement(1952..2446)
                     /locus_tag="VFH91_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863381.1"
                     /translation="MRAEDYAQLIRDQLGDAWLAQIYGRILKLRTRSYHFDLKRKSNL
                     ISIQHTLLGIELKIGTKRFACPDLAHARYLAVFARIGSEDVAVPYDITKVSLLADELE
                     SSWYRMLLLADEEAKGKPKSFKSRLRGMLATRLREEISAVGAGAKMPSFEKNTKGKGK
                     AIKP"
     gene            complement(2482..>3271)
                     /locus_tag="VFH91_10045"
     CDS             complement(2482..>3271)
                     /locus_tag="VFH91_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6863382.1"
                     /translation="AGPKAAGFASFMRVFIFGFPFVAAASNLTGARLHEAWVEVLVVL
                     AILTMTIGNVVAIVQDNVKRMLAYSSIAHAGYALVGFVAAGAASDIAQRDSAITAVMF
                     YLLTYAVMNIGAFTVVQLIARAGDRRTSIEDYSGIGFESPVLAFSFSLFMLSLLGMPL
                     TAGFMGKILVFGVAVDRGYYALVIIGVLNTAISAYYYLRLIIVMFFRDRTTSWVAPPV
                     PASIAIALVLSVLGVLYLGLFPGRVINALHTKIEAQAISQSSAP"
BASE COUNT          829 a          796 c          845 g          801 t
ORIGIN      
        1 tcgccgcatg ctggttcagg agaataaatg gcgtgcagtc cgctatggtc tggaaggcaa
       61 gttgatcgat tttggcaagc agatagaact accggccggc gaactagtac tggaattgct
      121 tgaattcgtt gatgatgtgg ttgacgagct gggttcgcgt caggagattt cgcacgttca
      181 tacaatcctg gaacgaggaa cgtcggcgga tcgccaacta aaagtatttc gcgaaactaa
      241 cgaccttaaa gccgttgttg atgatttgat ggagctgaca ttggaaaacg ttccggtgag
      301 tacggacgtg ggaataagtt cacccaaaac aacaacgacc tctacgattt cgaagtagca
      361 gcttttgatc tcggtacaga attgcgtgcc gctgctttgc gttcgagata gaagtgccag
      421 agcatccttg ccgcgactgc tcgatgaggc cgccaaccct ctgctaactc ttccaaaact
      481 tttggcggcg gaatttctgc caagcctttc aggtctcgta tagcaatggc cagggccaga
      541 tctcctgcgg gccaaatatc tggtcgacgc atcgccatca atagataaac atctgcactc
      601 catgagccga tacccttcac ctccattaat gctcggcgag catcgtcgtc gctcattctg
      661 gatagggctg caaggttcaa ctgtttcgta acgattaact ttgcaagatg aatgatatag
      721 ctggcctttt gacgcgttac tccattctct cgcaggtaac tttctccaag ctcacagaac
      781 cgttcaggcg tgaacggcac gacgatcgct ttgagccgat tgagcatcga cttagccgac
      841 agcagtgaaa cttgttgctc aaggatgatg tgaacaagcg ttgcgaaccc tgtacgtctt
      901 tgccagagcg gaggactgcc aaaatgaatg tggatgcggg cgagatccga atcacggcgc
      961 gtaagcgtta gtgcgatctc ttttaaagac tcgtcgttga gaagctgtga tcgagacacg
     1021 acaacctagc tcttgagtgc ttcaatcaag aagttctcaa acaggatgcg cccgtcacgt
     1081 gaatcattcg gatgttccca acgggtgcgg ttcttgttcc agtcctcaaa cgatttttcg
     1141 agatgaaact ggaccgtgta gatgagttga ccttcgcttc gtttaaccat catctggttg
     1201 cgacatagat aagatgtagc cagcagcttg aagcctacgg gcacgcgatc gagttgcagt
     1261 ccgtgattct gccagaccct gaggatgcga ggcacattgg agccgtcaga atcagtgagt
     1321 ggtatgtgtg taaagatcgg atcggaaggg tccgtaattc taacgaagcg atattgatac
     1381 tcgtgtgttc tcgcctcgcg ctgctcgtgt tgttccagcc ggtcaagggt gataactcga
     1441 gccccaaagc tcaagccaac gagctgatgt ccgccgcaga ttgccagaac cggtatatca
     1501 gtcttacgaa tgaagtcacc aaaatgatcc agaatggact gttcgtagta atcgaaatca
     1561 cggagagtgc cgctcaatac aattgcatca ggtttgaagt cacttgcggc tcgggctact
     1621 tctgaaacat gggcaacttc tgttttaggt tccttaatta atcgagaaat attgttgacg
     1681 atattttcga ttgccatccc agctatgact cgttcacgtt ttagctgact ttgtaatagc
     1741 tcgccccatt cgcgccgtgc caccggagaa atgtcagctt cgtccctgag tagattatcc
     1801 actacgagca cgcgcgcttt cgcgatgtgc tgttcgattg gttcgtatcg gcaaaaggtc
     1861 tgctggttaa aaggaaaggg aagtgtgctg gttacttcgg taaggtcatt gtcagattta
     1921 gtagctgaga gttcttgagt catttgttct gctaaggttt gatcgctttt ccttttcctt
     1981 tggtgttttt ctcaaagctg ggcattttgg cgccagctcc cactgctgaa atttcctctc
     2041 gcagcctggt agctaacatg ccgcgcaagc gagacttgaa cgatttaggt ttcccttttg
     2101 cctcttcgtc cgccagcagc aacattctat accaggatga ctcgagttcg tctgccagca
     2161 gcgacacttt tgtgatgtcg tacgggacgg ccacatcttc cgaaccgatt ctggcgaaaa
     2221 ccgcgaggta tctcgcatga gccagatctg gacaggcaaa tcgtttagtc ccgatcttta
     2281 gctcgattcc cagtaacgta tgttggatag agatcagatt tgactttcgt ttcaagtcaa
     2341 aatggtaaga gcgggtccga agtttaagga tgcgaccata gatctgtgct aaccaggcgt
     2401 cgcccaattg atcccgaata agttgagcgt aatcctcggc tcgcatttaa agtggaaaat
     2461 aatgtcgcct tgaaggtcaa attacggggc tgaggattgg gaaatagcct gcgcctcaat
     2521 cttagtatgc aaggcgttaa tgacccgacc gggaaataat cccaggtaaa ggacacccag
     2581 caccgacagc acaagcgcta tcgcgatact ggcgggaaca ggaggcgcaa cccatgaagt
     2641 agttcggtcc cggaagaaca tgacgatgat aaggcgcagg tagtagtaag ccgagatggc
     2701 agtgttgagc acgccgatta tgacgagtgc atagtagcca cggtcgacgg cgacgccaaa
     2761 aaccaaaatc ttgcccatga agcccgccgt caatggcata ccaagaagac ttagcataaa
     2821 aagcgagaac gaaaatgcta acacgggtga ttcaaagccg attccgctgt aatcctcaat
     2881 agatgttcga cgatcgccgg cgcgggcaat caattggacc accgtgaatg ctccaatgtt
     2941 catgactgcg taggtcaaga ggtaaaacat tacggcggtg atggctgaat ccctttgtgc
     3001 gatgtctgaa gcagcgcctg ccgccacaaa gccgaccagc gcataaccag cgtgcgcaat
     3061 tgacgagtaa gccaacattc gcttcacgtt gtcttgaact atggcaacca cgtttccaat
     3121 cgtcatggtc aagattgcca ggaccaccag aacctcgacc caggcttcgt gtaatcgcgc
     3181 accggtgagg tttgaagctg cggccacgaa tggaaaacca aaaataaaga cccgcatgaa
     3241 tgaggcgaat ccagcagctt ttggtccagc t
//
LOCUS       DASSFP010000446         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1322412, whole genome shotgun sequence.
ACCESSION   DASSFP010000446 DASSFP010000000
VERSION     DASSFP010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322412"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..>3000)
                     /locus_tag="VFH91_10050"
     CDS             complement(148..>3000)
                     /locus_tag="VFH91_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6863383.1"
                     /translation="NNSDSFIELPAGDIAAQLLGCLHPRFEPGQHIENYEIIRQLGSG
                     GMGEVYLAQDLRLGRQIALKLLPVEFTTHREHVRRFEFEARAASALNHPNIITIHEIG
                     RSDTSHFIATEYVDGLTLRQYMAGLPSEFRMSIAQVLDIAIQIGSALAAAHAAGIVHR
                     DIKPENVMLRHDGFVKVLDFGLAKLSRQIVTTETAMVNTNPGVVMGTVRYMSPEQARG
                     FEVDSRTDIWSLGVVLYEMLTARVPFRGATPSHVIVSILEKTPVALSTYSEVPPELER
                     IVTKALRKDRGRRYQSAGDLVLELKNLKQELETQAHLQKPLRQLRKGFDLLAGDEVDT
                     AEDVLAASTVRLDAPISTSTNPHPAKSPLNYMKLAALGALILAVGFGLYKFVTRNDSS
                     LAPTAPFQGIELLRLTNSGRVSDSAISPDGKYVSYVVESGGKQSIWIREVANSNNTQI
                     VAPKDSMFYGGTFSREGDYLYYIDKERNNTIGTLYRVPSLGGVPVKLIKDVDGPIALS
                     PDEKQLAFVRGASTGERALMLANVDGSGERKLASRMGYESFSFSGPAWSPDSKGIVCG
                     AAYTLENERFLTLVQVDISDASIRQLTTQKWKAIGRVTWLHDGKGLLFTATEQRAGST
                     SQLWYMDYPSGKAYRISKDLQDYHGASVTTDARTLVTKQTETLSSLWIAPNGDANLAT
                     EILSHKDDDGFNNSYYYRTRFSWMPDGRIMYTSLVNGTPSLWVMKATGSEAKQITSDT
                     SDSSFPSVTPDSRYIIFVSETLGFSKIWRMNIDGSNRKQLTNGPDESWSWCSPDSQWV
                     VYHSGNQGRRTVWRVPIEGGTPQQLTDYPSVTPVVSPDGQWISIYYRSETKAPWRLGI
                     IPFNGGPPVKSFEVPQGVLFQSLVRFTPDGSALAYIMNKDGSSNIWIQPLDGGPARQL
                     THFNSDQIFWFEWSPDGKQLGVSRGAITSDVVMIRR"
BASE COUNT          700 a          770 c          772 g          758 t
ORIGIN      
        1 aattcagtga gccactccaa gttttctgtt ttgaaataga tttgatcgta aggtcagtca
       61 gcgggggcgg cgctgtagtc gagtattgag agtaatcaca ttcgccgctc tgcccccttc
      121 ccaactgcat gattctggga tgttgcttta tcttcttatc attacgacgt cgctggtgat
      181 cgcgccacgc gagacgccga gttgcttgcc gtcgggtgac cactcgaacc agaagatctg
      241 atctgaattg aagtgtgtta gttgcctggc gggcccgccg tccaatggtt ggatccaaat
      301 attggaactg ccatctttgt tcatgatgta ggcgagcgca gacccatcag gtgtaaagcg
      361 cactaaagat tgaaacaaga caccttgcgg aacttcaaaa cttttgacgg gtggtcctcc
      421 gttgaaggga attattccca gtctccacgg cgctttggtc tctgaacggt agtagatgga
      481 aatccattga ccatcgggtg agaccactgg agtgaccgaa ggataatccg tcagctgttg
      541 aggtgttcca ccttcaattg gcactctcca cacagttcgc cttccttgat tgccagagtg
      601 atatacaacc cattgactat caggcgagca ccaggaccag gattcatccg gaccattggt
      661 gagctgtttg cggttgctgc catcaatgtt cattctccaa atcttggaga agcccagggt
      721 ttcggatacg aaaatgatat agcgcgaatc aggtgttact gaaggaaatg aactgtcgct
      781 tgtgtcgctg gttatttgtt tggcttcaga ccctgttgcc ttcattaccc agagacttgg
      841 agtaccatta accagcgagg tgtacatgat cctgccgtct ggcatccagg aaaaccgggt
      901 cctgtagtaa taggaattgt taaagccgtc atcgtctttg tgagacagga tctcagttgc
      961 aagattggca tcaccattcg gcgcgatcca aagactcgag agagtttccg tctgcttagt
     1021 aactagggtt ctggcatcag tagtgacact ggcgccatga tagtcctgca aatctttgct
     1081 gatgcgatac gctttaccgc tcggataatc catataccat agttgtgagg tcgacccggc
     1141 gcgctgctct gtcgccgtaa acaagagccc ttttccgtca tgtagccatg tgacgcgccc
     1201 gatggccttc catttctggg tagtaagttg tctgatcgaa gcatcgctga tatctacctg
     1261 gaccaacgtt aagaaccgct cattctctaa ggtgtacgcg gcgccacaaa cgataccttt
     1321 gctgtccggt gaccacgctg gaccactgaa agagaaggat tcatacccca ttcgcgatgc
     1381 aagcttgcgc tcaccactac catctacgtt cgcgagcatc aacgctcttt cgcccgtgct
     1441 tgccccgcga acgaatgcaa gttgtttttc atcaggtgat agtgcgatcg gaccatccac
     1501 atctttgatc aacttgacag gaacgccacc cagtgaaggc actcgataga gcgtaccgat
     1561 agtgttgtta cgttccttat caatatagta gaggtaatcg ccttctcgag aaaaagttcc
     1621 accgtagaac attgagtctt tgggtgcgac aatctgcgtg ttattggagt tcgcgacttc
     1681 tcgtatccaa atgctttgct ttccaccgct ttcgacaacg taagagacat acttgccgtc
     1741 cggagaaatg gcagagtcgc ttaccctccc ggaattagtc agtcgcagca actctatccc
     1801 ctggaaaggg gcggtgggcg ccagggagga atcattacga gtaacgaatt tatataggcc
     1861 gaaccccacg gccagaatta aagcgccgag tgccgctagc ttcatataat ttagagggct
     1921 cttcgccgga tgaggattgg ttgaagttga tattggcgcg tcgaggcgaa cggtacttgc
     1981 cgccaaaaca tcctctgcgg tgtcgacttc atcaccggct aaaaggtcaa acccttttcg
     2041 cagctgtcgt aagggtttct gtaaatgtgc ttgagtctcc agctcctgtt tcagattctt
     2101 tagttcaagc acaaggtcgc cagcactctg ataccgacgt cctcgatctt tacgcaaagc
     2161 ctttgtgacg atccgttcca gttccggcgg cacttccgaa taagttgaga gcgcaaccgg
     2221 agtcttttca agaatagaga caatcacatg actgggagtt gcgcccctga agggaactcg
     2281 agcggttaac atttcataga gcactactcc cagcgaccag atgtcagtgc gagagtcaac
     2341 ttcgaatcca cgcgcttgct cgggcgacat gtagcgaaca gttcccatca cgacgcctgg
     2401 attggtgttt accatcgccg tttctgttgt cacgatctgg cgcgacagct tggcgaggcc
     2461 gaagtccaaa accttcacaa atccgtcgtg tcgcaacatt acattctctg gcttgatgtc
     2521 gcggtgcacg atcccagctg cgtgcgctgc ggctagagcc gaaccgatct gaattgcgat
     2581 gtcgagcacc tgggcaatac tcattcgaaa ctcggacggt aatccagcca tgtactgacg
     2641 tagcgttaga ccatcgacat actcggtggc aatgaagtgt gaggtgtccg atcgcccgat
     2701 ttcatggatg gtaatgatat tggggtggtt gagtgcggaa gcggcgcgag cttcaaactc
     2761 aaaccggcgc acatgttcac gatgtgtcgt gaactcgacg ggcagcagtt tgagagcaat
     2821 ctgtcggccc aatcttaaat cctgtgcgag atagacctca cccatcccgc ccgagcccaa
     2881 ttgccgaatg atctcgtaat tctcgatgtg ttgtcccggc tcgaatcgcg ggtgaagaca
     2941 gcccagtagc tgggcggcaa tgtcgccggc tggtagctca atgaagctgt cagaattgtt
//
LOCUS       DASSFP010000447         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1766109, whole genome shotgun sequence.
ACCESSION   DASSFP010000447 DASSFP010000000
VERSION     DASSFP010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766109"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..951
                     /locus_tag="VFH91_10055"
     CDS             151..951
                     /locus_tag="VFH91_10055"
                     /inference="COORDINATES: protein motif:HMM:NF022450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase Amj family protein"
                     /protein_id="HET6863384.1"
                     /translation="MNRQVTVVFLLTFIINLIGTLAYALRIAGVRTRRIAVSLAVFNI
                     LLLVSRTANTFQGPLLAKHIERNILTGPFSNVEHDLRWLLLAASLATFAGVFLIPTFQ
                     RLFAKTITSLDTYRSLPRLALRIFYRSTWRQVRELASVPSRQNLTQLRFAEAPRAILI
                     FNMLATAFLTVGVFSSLYAGYLKPELRVTANNLSPIINAFSTILLFVFIDPYLSFLTD
                     DVVNGKATEARFRSCVFWLASSRLAGTLLAQLLLVPAAILIVKVAELL"
     gene            963..1889
                     /locus_tag="VFH91_10060"
     CDS             963..1889
                     /locus_tag="VFH91_10060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6863385.1"
                     /translation="MNEPVVSVVMPVHNARDFVDESIRSILGQTLQDFHFIILDDAST
                     DGSNEIIEKWAKRDSRIEFYKSERCLGLTGSSNLIVAKARAPIVARMDADDISHPDRL
                     KRQVELMQRNPDIGAVGTLCIGIDAFGREIRPRDRWRVVRRSRYVPFSHGSATFRKDL
                     FDAIGGYRECNGAGEDQDLFTRMAQVSTVVTLPDVLYSYRYHTSNATFNNSITSIKQN
                     GNGNGNNLASYYMLGAMSLWAGQKPQVLKHMLNEPSLKWTPNTLVTLASAAWGSFHPG
                     SLRSFLRLLIRTRDMVAGFSVEEGRPYKWRLK"
     gene            1874..2893
                     /locus_tag="VFH91_10065"
     CDS             1874..2893
                     /locus_tag="VFH91_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6863386.1"
                     /translation="MAIKIAITGAGLRGRDWIKEVNTASDFELVACVDTDPATLEATA
                     RQFHFSQESCFSDLEEAIKSIECDAVIVVTPADQHFKDCYTALSHDKAVLVEKPFTTT
                     LRAAHQLVNLAEAKQLPLIVAQNYRYLRSYRTVRQLLKEGRLGRVGIVVCQYYRIPHE
                     MSLAQKTTTQSVLWGIGVHHLDALRYVLDDVVTNVMSDDFTLSWTNLPRGASIRTMLS
                     FDKGTRCFYTATYESRGHEFFENGQEFYARFVGELATLHVFQRWLILCEKGKLPRLLR
                     RGSRKITEEQTLLNQLKQAMRGTDHPETSGRDNLQTMALLEACLISSAKQTWTNPQEL
                     LDELD"
BASE COUNT          778 a          749 c          762 g          752 t
ORIGIN      
        1 ccatgaaaat ggtgaccgcg acgaaaaggt gagaacttta cggataagag agtccaaaac
       61 acgactttca tttgctagaa ttccgctgaa taggtacaac acaaaagtag aaatacccag
      121 caaaggttca cagcaccaga cacgagcaat atgaaccgtc aggtaaccgt tgtcttctta
      181 ctgacattca tcatcaacct gattggcacc ctcgcttatg cgctccgtat agcaggcgtc
      241 cgcacgcgac gaatcgcagt ctcgctagcg gtcttcaaca tcctgctact cgtctctcgc
      301 acagctaaca cctttcaagg tccgctactg gccaaacaca ttgaacgaaa tatattgacc
      361 ggtccatttt ccaacgtcga gcatgacttg cgttggttac tgttagccgc aagcctggcc
      421 actttcgctg gagtatttct gataccgact tttcaacgct tatttgccaa gactattacg
      481 tctctggata cctatagatc tttgccgcga ctcgcactac gaattttcta tcgatccacc
      541 tggcgtcaag taagagaatt agcaagcgtc ccttcaagac agaacctcac tcagttgcgt
      601 ttcgctgagg cgccccgtgc gattcttatt ttcaatatgc tggcgacagc atttctcact
      661 gtcggagttt tctcgtcgct ttatgcggga tacctgaagc cggaattgag agtgaccgct
      721 aacaatttgt caccgattat caatgcgttt tccactattt tgctttttgt tttcatcgat
      781 ccttacctgt cattcctgac cgacgatgtg gtgaatggaa aggctactga agcccgattt
      841 cgaagttgcg ttttttggtt agcatcgagt cgtcttgctg gaacgttgtt ggcccaacta
      901 ttacttgtgc cggcagcgat cctaattgtg aaggttgccg aacttctgta gagaattaag
      961 gaatgaacga acccgtcgtc agcgtcgtga tgccagtgca taatgcgcgg gatttcgtcg
     1021 acgagagcat tcggagcatt ctgggccaaa cgcttcagga ctttcatttc ataatcctgg
     1081 acgacgcgtc gaccgacggc tctaacgaga tcattgagaa gtgggcaaaa agagactcgc
     1141 ggattgagtt ctataagagc gaacggtgcc ttggtcttac tggaagttcc aatcttatcg
     1201 tagcgaaagc aagggccccg atcgtcgccc gaatggacgc agatgatatt tctcatcctg
     1261 accggctgaa gcgccaggtc gaattgatgc agcgaaaccc agacattggc gcggttggga
     1321 ctctctgtat agggatcgat gcgtttggcc gcgaaatccg ccctcgcgat agatggagag
     1381 ttgttagacg ctcgcgttac gtgccctttt cacacgggtc ggcaactttt cgtaaagact
     1441 tgttcgacgc aatcggcgga tatcgggaat gcaatggtgc cggagaagat caagatctgt
     1501 ttacgaggat ggcccaagtt tcaactgtag taacgcttcc ggacgtactc tacagttacc
     1561 ggtatcacac cagcaacgct acgttcaata acagcataac ttcgatcaag cagaacggaa
     1621 acggaaatgg aaataacctc gcctcgtatt acatgctagg ggcaatgtcg ctatgggcgg
     1681 ggcaaaaacc gcaagtacta aaacatatgc tcaacgagcc atcgctcaaa tggaccccga
     1741 atactctggt gacgctcgcg agcgcagcct ggggtagttt ccatcctggc agtttgagat
     1801 cttttctgcg cttgttgatt cgaaccaggg acatggtggc cggtttcagt gtcgaagaag
     1861 gtagaccata caaatggcga ttaaaatagc gatcaccggg gcgggtttac gcggacgaga
     1921 ttggatcaag gaggtcaaca ctgcaagcga cttcgagttg gtcgcgtgcg tcgacaccga
     1981 cccggcaacg ctcgaagcga cagccagaca gtttcacttt tcgcaggaaa gctgtttcag
     2041 cgatctcgaa gaagcgatca aaagcataga atgtgatgcc gttatcgtag tcacgccagc
     2101 cgatcaacat ttcaaagact gctataccgc tctctcgcac gataaggcag ttttagtcga
     2161 gaagcctttc acgacaactt tgagagcagc ccatcagctg gttaaccttg ccgaagctaa
     2221 gcagctacct ctgattgtcg ctcaaaatta tcgatatctt cgctcgtatc gaactgtgcg
     2281 tcagttattg aaagaaggac gacttggacg cgtaggtatc gtggtctgcc agtactatcg
     2341 aatacctcac gaaatgtcgt tagcccaaaa gaccacaacg cagagcgttt tgtggggtat
     2401 aggcgtacat catctcgacg cgttgagata tgttcttgat gatgttgtca cgaatgtgat
     2461 gtctgatgac tttacgctct cgtggaccaa ccttcccagg ggtgcttcca tcagaacaat
     2521 gctttcgttc gacaaaggaa cacgatgttt ctacaccgcg acttacgaat cgcgaggtca
     2581 tgagttcttc gaaaatggac aggagttcta tgctcgtttt gtcggcgagc tggccactct
     2641 tcacgtgttt caacgatggc ttatattgtg tgaaaaggga aaactgccac gcctgctgag
     2701 acgcggcagc aggaagatca ccgaagaaca aacgttattg aatcaactca aacaagccat
     2761 gcgaggaacc gatcatccgg agacgtccgg acgtgacaac ttgcagacca tggctttgct
     2821 ggaagcttgc ctgatatctt ctgcaaaaca aacatggacc aatccccagg agttgctgga
     2881 tgagctcgac taacggacgt cctaaggtgc tggccattgg acttgatgcg gcagaagcga
     2941 cgttaataag agaattgatg gatgctggtc agatgcctgc aatggcgtcg ttggcgcgta
     3001 atggacaatg gcttcagttg aaagctcccg cttacatcgg c
//
LOCUS       DASSFP010000448         7326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_134109, whole genome shotgun sequence.
ACCESSION   DASSFP010000448 DASSFP010000000
VERSION     DASSFP010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134109"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..629)
                     /locus_tag="VFH91_10070"
     CDS             complement(279..629)
                     /locus_tag="VFH91_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863387.1"
                     /translation="MSLRRRSAVFAILACTVFLTACPSQTNVARINADPGRYRGKEVA
                     IAGKVTDSYGFMGAGGYEIDDGTGKMWIATTRGVPSRGALVGAKGYVRTGLTFSGRTF
                     GTVIEETERHAKGK"
     gene            complement(745..2253)
                     /gene="betB"
                     /locus_tag="VFH91_10075"
     CDS             complement(745..2253)
                     /gene="betB"
                     /locus_tag="VFH91_10075"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009725.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HET6863388.1"
                     /translation="MSSAQLVENNTPRKYQLFIDGQWVDAESGKTFATPNPATGETLA
                     EVAEADKADIDKAVKAARRAFEGKWSKISARDRGRLLYKLSQLLEEKSADLAAIETAD
                     NGKPIKESLYIDLPQVVENFEYFAGWATKIEGETIPVPGKMFNYTLREPIGVCGQIIP
                     WNFPLLMAAWKLAPALAAGNTIVLKPAEQTPVGAMELASLIQEAGFPEGVVNVVPGYG
                     ETAGAALAAHPGIDKVAFTGSTEVGKLVAKAAAENLTKVSLELGGKAPNIVFADSDLD
                     QAVAGAMMGIFYNQGQVCCAGSRLFVEASIKDDFVGKLKERAERIAVGDPMDKGTQMG
                     PQVSAEQLDRIKGYVDIARSEGASVLTGGAPPSLDPAFEKGFFFRPTIFADVNNKMRV
                     AQEEIFGPVVSVISFENEEDLIKQANDTIYGLSAGIWTRDITRAHRFAKEIKAGVVWI
                     NTYNMFNAASPFGGYKQSGYGREMGKHALDLYTQVKSVWVDLSGRPIGWFGK"
     gene            complement(2277..5156)
                     /locus_tag="VFH91_10080"
     CDS             complement(2277..5156)
                     /locus_tag="VFH91_10080"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET6863389.1"
                     /translation="MPDSPFNSLQTLESDSGPAGQFYSLPHLERSGVGPISRLPISIR
                     IVLESVLRNCDGRKITEANIKALANWQPNAQRVEEIPFVVARVLLQDFTGVPLLVDLA
                     AMRSAVARLGKNTELIEPLVPVDLVIDHSIQVDFSSIKDALEKNMEMEFKRNRSRYQF
                     LKWGTQAFNGFRVIPPGIGICHQVNLEYLARSVWDKNVLFPDTLVGTDSHTTMINGLG
                     IVGWGVGGIEAEAAMLGQPVYFLTPDVVGVNLTGALREGVTATDLVLRVTEMMRKAKV
                     VGKFVEFHGKGAASLSVTDRATIANMAPEYGATMGFFPIDEETCGYLLATGRPEEHVD
                     LVRRYYQAQGMFGIPGKGECDYSVVYDLDLGSIEPSVAGPKRPQDRIELPKLKDQFEQ
                     LLVKPIADGGYGKSHDELWTRYHVKIGADRQRNIAGGGEQATDSIPAISKDSNSVANT
                     SVWTETEMVNNRPTPDLVPEIPEEEFPGGDELIGHGDVLIAAITSCTNTSNPSVMLAA
                     GLLAKKAVERGLTVSPKVKNSLAPGSRVVTEYLERTGLQPYLNELGFNLAGYGCTTCI
                     GNSGPLDPALEQVVNERDVIAASVLSGNRNFEARVHQSIKANFLMSPPLVVAFALAGR
                     IDLDLSSDPIGTGKNGTDVYLRDIWPSLQEINDLMRAAFDPETYRRLYGNFADQNPLW
                     NDIPMSSGSVYEWEPESTYIREPPYFEKFSSKLESLADIKGARPLAIFGDSVTTDHIS
                     PAGAIKASSPAGLYLQERGVDIRDFNSYGARRGNHEAMVRGTFANVRIKNLMVPGVEG
                     GVTIHQPSGDRTSIYDASMRYQAEGVPLVIFAGQEYGTGSSRDWAAKGTRLLGVRAVV
                     AQSFERIHRSNLVGMGVLPCQFKDETSAATLRLDGSETFDLVGIEAKLKPLQDVTLVI
                     HRASGAIEEIAVTLRIDTPIEVDYYRHGGILPYVLRQLLADTTDY"
     gene            5434..5832
                     /locus_tag="VFH91_10085"
     CDS             5434..5832
                     /locus_tag="VFH91_10085"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HET6863390.1"
                     /translation="MPRKHAQDFEYLANDPNYVPDPEIEEFMESEISRVPRDSDLLAH
                     RLRNNTAASPRDAGGDLDADWEGVNESGSETFAGDNPTPDQSLVEENAHAMGVSFEDN
                     EELDFLDKIEKRDRERFELEERSKGSDDMI"
     gene            complement(5836..6285)
                     /gene="dtd"
                     /locus_tag="VFH91_10090"
     CDS             complement(5836..6285)
                     /gene="dtd"
                     /locus_tag="VFH91_10090"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET6863391.1"
                     /translation="MRAVVQRVTRASVNVDDQIVGAIGRGLLVLLAVANDDQKSDADY
                     LVEKISALRIFDDAEGRMNVSLKDSGGSLLIVSQFTLYGDVRRGLRPSWSDAAAPEKA
                     EPLYDYFVERGRDLLGEVQTGSFRKMMQVELVNDGPVTVLLDSKKVF"
     gene            complement(6437..7150)
                     /locus_tag="VFH91_10095"
     CDS             complement(6437..7150)
                     /locus_tag="VFH91_10095"
                     /inference="COORDINATES: protein motif:HMM:NF005594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6863392.1"
                     /translation="MELQNKVAVVTGGTKGIGNAIAKSLAQAGASVVVTSRHRDQVDE
                     AVDKLREVAADRVTGFACDVRQYDQVESLIRGTLERFGSLDILVNNAGIGMFGTVAEM
                     DPEDFRAVIETNLLGVYYCCHEAIPHMKQRGGGYIINISSLAGTNAHPEMAAYNASKF
                     GLNGFSEALMQEVRHDNIKVSYIMPGSVNTEFGDDEPDASKSWQLQPEDIAEVVMDLL
                     AHPDRSLPSRVEIRPSKPR"
BASE COUNT         1797 a         1996 c         1731 g         1802 t
ORIGIN      
        1 ttccggtaag gtccactcga acgggtcatc ccaaagatta gtggtaaggc cgccaaatgt
       61 ttgttcgaga atggctgcac tacgaagaat gttctcgcca aacgtgatgg caggcggacg
      121 cttgtacaac aaatccgggg aaacggacta aactaaaacc ttcagacgat cgatcaagcg
      181 cgcgaaagca agatcaagtt ctttgacaat ctcgcgctgc tctgcggttt tgttttccat
      241 gtcggaaacc accgcgaccg aaaccttgcg cctccctgct atttgccttt ggcgtgacgt
      301 tcagtctctt cgattacagt tccgaaagtt cttccgctaa acgtcaatcc agtcctgaca
      361 tagcccttgg ctccaactaa tgcgcctcgg gagggaacgc ctcgagtagt cgcaatccac
      421 atctttccag tgccatcatc gatctcgtaa ccaccggccc ccataaatcc gtaactgtcc
      481 gtgacttttc cggcaatagc gacttcttta ccgcgataac gccctggatc tgcgtttatc
      541 ctggcaacgt tggtttggct gggacaggca gttagaaaaa cagtacaagc taggattgcg
      601 aaaacagcgg atcgtcttct caatgacatt gcatctcctc caatgagcat ttaaccatct
      661 cgggagagtt tgactttggg atgatagcat tggttgatag aggtcctata ggtcttacag
      721 gactcatagg acccataaac ccctttactt tccaaaccag ccgattggcc gacccgatag
      781 atccacccac acgctcttta cctgcgtata cagatcgagc gcgtgcttac ccatttcacg
      841 gccataacct gactgtttgt aaccaccaaa aggcgaagca gcattaaaca tattgtaggt
      901 attgatccag accacgccgg ctttgatctc tttggcaaac cgatgcgcgc gcgtgatgtc
      961 gcgagtccag atgcccgccg acaatccgta gatcgtgtcg ttggcctgct taatcaagtc
     1021 ttcctcattc tcaaagctga tcaccgagac caccggacca aaaatctctt cctgggccac
     1081 ccgcatctta ttgtttacgt ccgcaaaaat tgtaggacgg aaaaagaaac ccttctcaaa
     1141 cgccggatcc aggctcggcg gagcgccgcc tgttagtacg gacgctcctt cggatcgagc
     1201 aatatcgaca taacctttaa tgcggtcgag ctgctcagcg gacacttgcg ggcccatttg
     1261 cgttcctttg tccataggat cgccaacggc aattctttcc gcacgctctt ttaacttacc
     1321 gacgaaatca tccttgatac tcgcttcaac gaaaaggcga gaaccggcgc agcaaacctg
     1381 gccctgattg tagaaaattc ccatcatcgc tccagcgact gcctgatcca gatcggaatc
     1441 agcaaagaca atgttgggcg ccttaccacc gagttcgaga gaaacctttg ttagattctc
     1501 agccgcagct ttggcgacaa gttttcccac ctcggttgaa ccagtgaatg ctactttatc
     1561 gattcccggg tgggcggcga gtgcagcgcc agctgtctct ccgtaaccgg gtacaacatt
     1621 tacgactccc tcagggaagc ccgcttcctg gatcaggctc gccaactcca tcgcgccaac
     1681 gggtgtttgt tccgcaggct tcagcacgat cgtatttcct gctgcaagtg ccggagcaag
     1741 tttccaggcg gccattagaa gcggaaaatt ccacgggatg atctgaccgc acacacctat
     1801 cggctcgcgt aaggtgtaat tgaacatctt gccgggtact ggaatcgtct cgccttcgat
     1861 cttagtggcc caacctgcaa agtactcaaa gttttccact acctgcggca agtcgatata
     1921 taacgactct ttgattggtt ttccgttgtc ggcggtttcg atcgctgcaa ggtcagccga
     1981 cttctcttcc aatagctgcg aaagcttgta taaaagtcgt ccccggtcgc gagcacttat
     2041 tttggaccat ttgccttcaa aagcacgacg agctgccttt acagccttat cgatgtcggc
     2101 cttgtcagct tcagcgactt ctgcaagtgt ttcgcctgta gcaggatttg gtgttgcaaa
     2161 agtctttccc gactcggcat cgacccattg tccatcgatg aaaagctggt acttcctcgg
     2221 cgtgttgttt tcaactagtt gtgcagatga catatggact ccctgggttt taacctttag
     2281 taatcggtgg tgtctgccag cagttgacgg agaacataag gcagtattcc accatgacga
     2341 tagtaatcaa cttcaatcgg cgtatcgatg cgaagcgtaa cggcgatctc ctcgatcgcg
     2401 ccactcgcgc gatgaataac aagcgttaca tcctgaagtg gctttaactt agcttctatc
     2461 cctaccagat caaaagtttc gcttccgtcg agacgaagag tggccgcgct ggtttcatct
     2521 ttgaactggc acggcagtac gcccatgccg acaagattgc tacggtgaat acgctcgaaa
     2581 ctttgtgcga ccacggctct gactccgagg agtcgcgttc ctttcgccgc ccaatcacgt
     2641 gaacttccgg tgccgtactc ctgtccggcg aaaattacga gcggaactcc ttctgcctga
     2701 tatctcatcg aagcatcata aatacttgtg cggtcaccac tcggttgatg aattgtgaca
     2761 ccaccttcga caccaggaac catcaagttc ttaattcgaa cattggcgaa agtaccccga
     2821 accatcgctt cgtgattgcc gcgccgcgcg ccataactat tgaagtcgcg aatatcaact
     2881 ccgcgctcct gtagatacag gccagcgggt gatgatgcct tgatcgcacc agccggactg
     2941 atgtgatcgg tagtcaccga gtcgccgaag atggcaagcg gacgagcacc tttgatatcc
     3001 gcgagcgact ctaacttgct ggaaaacttt tcgaagtacg gaggctcgcg aatgtaggtc
     3061 gattcgggtt cccactcgta aacacttcca gagctcatcg gaatgtcgtt ccaaagggga
     3121 ttctgatccg caaagtttcc atacaacctg cgatatgtct ccgggtcgaa tgccgctcgc
     3181 atcaggtcat taatctcctg cagactgggc caaatgtcgc gcaggtaaac atcagtgccg
     3241 ttcttcccgg tgccaattgg atctgacgac aggtcgagat cgatccggcc ggcgagagcg
     3301 aatgcaacta ccagcggagg gctcatcaga aagttcgcct tgatactctg gtgaacacga
     3361 gcctcaaagt ttcgattacc agaaagtacc gaagcggcga taacgtcccg ttcgttcaca
     3421 acctgttcga gtgccgggtc gagcgggcct gagtttccga tgcaagtcgt gcagccataa
     3481 ccggcgaggt tgaatcccaa ctcgttcagg taaggttgta aacctgttcg ctcgaggtac
     3541 tcggttacga ctctcgatcc aggcgccagc gagttcttaa cttttggact tactgtcagg
     3601 cctcgctcga cagctttttt agctaacaga ccggctgcca gcatcacact tggattcgaa
     3661 gtgtttgtgc acgaggtgat ggcggcaatc aaaacatcgc catgccctat caactcgtca
     3721 ccgcctggaa attcttcttc gggaatctcc ggcaccaagt cgggtgtggg acgattgttc
     3781 accatctcag tttctgtcca cacacttgta tttgccactg agttgctgtc tttgcttata
     3841 gcgggaatgc tatcagtcgc ctgttctccg ccaccggcga tgttgcgctg tcgatcggcg
     3901 cctatcttca cgtggtaacg agtccacaat tcatcgtgac tcttaccgta gccaccgtca
     3961 gcgattggtt tgacgagcaa ttgttcaaac tgatccttca acttcggaag ctcaattcga
     4021 tcttgaggtc gttttggacc agcgacactt ggttcaatcg agcccaggtc gagatcgtaa
     4081 acaacgctgt agtcacattc accttttcca ggaatgccga acattccctg cgcttgataa
     4141 tagcgacgaa cgagatcaac atgctcttca ggtcgtccgg tagcaagaag ataaccacag
     4201 gtctcttcat cgatcgggaa gaatcccata gtagcgccgt attcgggcgc catgttggca
     4261 atggtggcgc gatcggtgac tgacagactt gcggcgccct tcccatgaaa ctctacaaac
     4321 tttcctacaa ccttggcttt acgcatcatc tcagtgacgc gcaagacaag gtcggtggcg
     4381 gtaacacctt cacgaagcgc tccggtcagg ttcacgccaa caacgtccgg tgtcagaaaa
     4441 tagacgggct ggcccagcat tgctgcttct gcttcaattc cgcccacccc ccaaccaaca
     4501 atcccaagac cattgatcat cgtggtgtgg gaatcggtac caaccagcgt atctggaaag
     4561 aggacattct tgtcccaaac tgatctggca agatattcga gattgacctg atggcagatg
     4621 ccgatgcccg gcggtatgac tcgaaaaccg ttgaatgctt gtgttcccca ttttaggaac
     4681 tgataccgcg aacgattgcg cttgaactcc atctccatgt tcttttccag agcatcctta
     4741 atgctggaaa aatcaacctg gatcgaatgg tctataacca aatccacggg cacaagcggc
     4801 tcgatcagct cggtattctt gcctaaacga gcaaccgcgg atcgcatcgc tgcgaggtcg
     4861 acaagcagtg gaactccagt gaagtcttgc aacagcacgc gcgcgacaac gaaaggtatt
     4921 tcctcgactc gctgagcatt gggttgccag ttagcaagtg cctttatatt tgcctcggtg
     4981 atcttgcgac catcacaatt gcggaggact gactccagga ctattcgaat ggagatcggt
     5041 agtcgtgaaa ttggcccaac tccgcttcgt tcaagatgtg ggagcgaata gaattgtccg
     5101 gcggggcccg aatctgactc gagtgtttgc agagagttga acggactatc aggcatagag
     5161 gtgctcaaac cctttccagc gaatacgcat agatcgtgtc attaattttg tgattatgat
     5221 gcaaatttca gtgtaaagtc cgcctcacta atcgtcaagc aacgcgtttt gtaaggagtg
     5281 tcctaatcta aaacaatcaa gacaccacgg aggtgctacg ccagtgaggg tttgtgatat
     5341 agtctccgca attcgttccc ccaaaattga aacataagtg acgtagtctt gcaggaatgc
     5401 ttgtcacctg taagcctgcc gagggaggca gctatgccga gaaagcacgc acaagatttc
     5461 gagtaccttg ctaacgaccc aaattacgtg ccagaccctg aaatcgaaga gtttatggaa
     5521 tcggaaatct cacgggtacc gcgcgattca gatttgctcg cccatcgact gcggaacaac
     5581 acggccgcct ctccgcgcga tgctggtggc gatctcgatg cggattggga aggtgtaaac
     5641 gaaagtggct ccgaaacttt tgccggtgat aatcccacac cggaccaatc actcgttgaa
     5701 gaaaatgcgc acgctatggg ggttagtttt gaggacaacg aagagctgga ctttctcgac
     5761 aaaatcgaga agcgcgatcg cgagcggttt gaactggaag agcgctcaaa aggttcagac
     5821 gatatgatct gaaggctaga ataccttttt actatccagc agaaccgtta caggcccgtc
     5881 attaaccaat tcaacctgca tcattttgcg aaaacttccg gtctggacct cacctaatag
     5941 gtctctgccg cgctcgacga aataatcgta taacggttcc gccttttcgg gcgcagctgc
     6001 atcactccac gatggtctaa gtcctcgtcg cacatcacca tacaatgtga attgagagac
     6061 gattaacaac gagccaccag aatccttcag cgaaacgttc atgcgtcctt cggcgtcatc
     6121 gaatattcgt aacgccgaaa tcttttcaac cagataatcc gcatcagact tctgatcatc
     6181 gttcgcaacc gcaagcaaaa ccaataaacc gcgaccgatc gcgccaacga tctggtcatc
     6241 cacattcaca ctggctcgag ttacacgctg aactacagct ctcatctttg gagtgcgccg
     6301 gcccggcggc gctttgttat atagctatcg cacaaggtaa gtagatccaa aagaattgag
     6361 aattgagaat tgatatttga caattactat ttccgcgaaa ttagtaattc tcaaatatca
     6421 attctcaaat ctcacttcat ctcggtttac ttggtctaat ttcgactcga ctgggaaggc
     6481 tccggtctgg atgtgcgaga agatccatta ccacttcagc gatgtcctca ggttgcaatt
     6541 gccaactctt tgatgcatcg ggttcgtcgt cgccaaattc agtgttgact gagcctggca
     6601 tgatgtaact cacctttata ttgtcgtgac gtacttcctg cattagagct tcactgaacc
     6661 cgttcaaacc aaacttcgaa gcattgtagg cggccatttc gggatgagca ttggttccgg
     6721 ctagcgaaga gatattgatg atatagccac cgccgcgctg tttcatatga ggaatcgctt
     6781 cgtggcaaca ataatagacg ccaagcagat tcgtttcgat aaccgctcta aagtcctccg
     6841 gatccatctc tgcgacggtt ccaaacattc caatacctgc attgttgacg agaatatcga
     6901 gggaaccgaa gcgctcaagc gtaccgcgaa ttaaagattc gacctgatcg tactgacgca
     6961 catcgcaagc aaatcccgta actcgatcag ccgcgacttc tcgcagctta tccacggcct
     7021 catcaacctg atctcgatgg cgcgaagtta ctacaacact tgcgccagcc tgagccaggg
     7081 atttagcaat tgcatttcct atcccctttg tgccacccgt tacgacggca actttatttt
     7141 gcagttccaa tgttaacctt gaagcttgag cttcaaccga tcgttgacaa tctttggatt
     7201 ggccttaccc ttcgacgcct taatcacctg cccaacaaaa aatccaaaca gggcttcctt
     7261 gccggaacga tagctggcca gctgatcggg attcgaagcg attacttcgt cgatcaaact
     7321 gtcgat
//
LOCUS       DASSFP010000449         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_879051, whole genome shotgun sequence.
ACCESSION   DASSFP010000449 DASSFP010000000
VERSION     DASSFP010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879051"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2270
                     /locus_tag="VFH91_10100"
     CDS             <1..2270
                     /locus_tag="VFH91_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6863393.1"
                     /translation="PSWSLLETGQHLQIKGNVEFDRYTLHSYDYLNFFPLYVAGALGT
                     RIDMPAVEVLDSLGVPLITDAYAHDERYMTFSLFLRGPRYSTLQDSLQNRFRKSPKEL
                     FDEWTMGFELRSALTDQLVREMLQKLDNPKLKYLDLYLADFDHVAHHNNDLESQVAVL
                     KQMDAVIGEVWTAIQKTPLADETALVLVSDHGFNSDPRIYSQGYNLVKLLGSRAGGGH
                     HVITKRRLMLDYAIKGVNPLVPLITTTTRDSYYLQGQSSDYPTAMLDFDGNERASIHL
                     RDSDLNILHLILQQLQRSDLPPATRQAVIKAFFSTLDRRRPEWQTTLNQLNEELGALR
                     RRIEEQRKLVQAQPKKFTREEQQAGLDDASKRVFVQWDRWVGQEKEYSTYAGTLQNLL
                     NLTPDALNSPKLRISDFIARDSMGDRNSIYELQNYVVGLSPAGLVVGENGQLDMESSF
                     VRIDYLKLLQNVAVRNNVQPGINNRPVDIITTSISSKSIVPVIDDSRISEDVVWVTSG
                     TDKQALILSRTDEKGQLMLRYQPIRNLTQDQDGRVRFELIEWQPGLPLHIFEDPNVNI
                     PDGVEKLSWLRQWHTDLEWLRALHRALYSNGLVGLNEELTRHPIERLSLDEKGLAADE
                     MLMRRLAHRQRDIVVPDMLVVANNHWNFDVRGFNPGGNHGSFFRISTHSVLMVAGGAK
                     TNIPRAMEVTEPYDSLSFVPTLLALRGDLRDDSNPVPVLWDRGFRRFPGRLIKELLPA
                     NQDKSTIVTGASGSP"
     gene            2267..>4188
                     /locus_tag="VFH91_10105"
     CDS             2267..>4188
                     /locus_tag="VFH91_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET6863394.1"
                     /translation="MIESREELTENWPTDEGEEVSEESASSTSRNSRRSFVWLQTLAF
                     ILGLGLLVYVIRLVGIQPLFDALLRIGFGFFVILGLSGLRHVLRTISMRAAVPPEHRQ
                     ITFRQAFAARLGGEAISFLTFTGPLLGEATKVALLRKRVPLTYGVPALVVDNLIYNLS
                     VVFFVLSGACVMLLRYPMPTPVYLVLLAIALVAALGILTAIFAAKRRVMLLTWLIDRM
                     AQLRLSPKVVLKKRQHIYHLESKVYDFYKHHPGAFFGMIACNLLAHAASVAEVYLALR
                     FLGFQPHVAQAYIIESLTKVINFAFAFVPGTIGVYEGGTEVILQKGLGFTPAAGLALA
                     LVRKAAIVCWTSAGLLVLTSRTLPNAWRRILDRSPRLQRLMDSLVFSNIAHRPARTAV
                     SILGTGLGVLLIVFTVGLAHGVLHERGRRESSIGAEIMIRASGTISLGGSSPFRLPVS
                     HAAELAKVEGVKAATPIGQTLDRSDSGFGSRLIDGIEYNEYAALAGLSMREGRGLMSG
                     DEAIVDPEWKERRKTQIGEKVKLFERQFTIVGVYEPPGGGRIKIPLRTLQEQEGAEGR
                     ASAILVSCSDPAKQDEVAARILSQFPSDQLIFTRDLPDIYASGVPALNVFIKVVVGVA
                     AAISMLVILLAMYTTVT"
BASE COUNT         1018 a         1033 c         1081 g         1056 t
ORIGIN      
        1 caccctcctg gtcgctgctg gaaacgggtc aacatttaca aatcaaaggc aacgttgaat
       61 tcgaccgcta tacactccac tcttacgatt acttaaattt ctttccgttg tatgtcgctg
      121 gagctctcgg gactcgcatc gatatgcccg cagtcgaggt actcgattca cttggagtac
      181 cgttgataac cgatgcatat gctcatgacg agcgttacat gaccttttcg cttttcttac
      241 gtggtccacg atattcaact cttcaagaca gtctgcaaaa tcgcttcagg aaatccccca
      301 aggaattgtt tgatgagtgg accatgggat tcgagcttcg cagtgccctg acggatcaac
      361 tcgtcagaga aatgcttcag aagctcgaca atccgaagct gaagtacctg gatctgtacc
      421 tggccgattt tgatcacgta gcgcatcaca ataacgatct tgagtcacaa gtggctgtgc
      481 taaagcaaat ggacgcagtc atcggagagg tatggactgc aattcaaaag acaccgttgg
      541 cggatgaaac ggccttggtc cttgtttcag atcacggatt caactcagat cccaggattt
      601 acagtcaggg ttacaatctc gttaaacttc tcggcagtcg cgcgggaggc ggtcatcatg
      661 tgatcacaaa gagacgtttg atgctcgact acgccatcaa gggtgtcaat cctctggtgc
      721 cgctgatcac aactacaacg cgcgattctt attacctgca gggacaaagt tcggattatc
      781 cgactgcgat gttggatttt gatggtaacg agcgagcgtc aattcatctt cgtgatagtg
      841 acctcaacat acttcatcta atactgcagc agctacaacg ttcggatctt ccgccggcga
      901 cgcgccaggc agtaatcaaa gcgtttttca gcaccctcga tcgcaggcgt ccagagtggc
      961 aaacaactct caaccagctc aacgaagaac tgggtgcgtt gcgacgtaga atcgaggagc
     1021 agcgcaaact ggtccaggcc cagccaaaga aattcaccag ggaagaacaa caggccggcc
     1081 tggatgacgc ctctaaaaga gtctttgtcc agtgggatcg ctgggtgggt caagagaaag
     1141 aatactcaac ttatgctggt actctgcaaa atctgctcaa tctcactccc gacgcactga
     1201 actccccaaa gctacgcatt tcagatttca ttgcgcgcga ttccatgggc gatcgaaact
     1261 caatttatga gctgcaaaat tacgttgttg gactaagtcc agcaggtttg gtcgtgggcg
     1321 agaacggtca gctcgacatg gagagttcct ttgtaagaat cgattattta aaacttttgc
     1381 agaatgtggc ggttaggaac aatgtccagc cgggaatcaa taatcgccct gtagacataa
     1441 ttactacgag catttcatca aaatcaattg ttcctgtaat cgatgattcg agaatttctg
     1501 aagacgtcgt ctgggtcacg agcggcacag acaagcaggc gctgatcctg tcccgcactg
     1561 acgaaaaagg tcagctgatg cttcgctacc agccaatcag aaatcttact caagatcagg
     1621 atggtagagt tcgtttcgaa ttgatcgaat ggcaaccggg tttgccactt cacatatttg
     1681 aagacccaaa tgtgaacata cctgatggtg tcgagaagtt gtcgtggttg aggcagtggc
     1741 atacagacct cgaatggtta cgtgctttgc atcgcgcgtt atattcaaat ggtctggtcg
     1801 gacttaatga agaactgacg cgccatccta tcgaacggtt atctctcgac gaaaaaggcc
     1861 tggcagctga tgagatgttg atgagacggc tcgcgcaccg ccagcgagac atagtcgtgc
     1921 ccgatatgct cgtagttgcc aacaaccatt ggaacttcga tgtacggggc ttcaatcctg
     1981 gcggcaacca cggatcattc tttcgtatct ccacgcactc agttctgatg gttgctggag
     2041 gtgcaaagac caatataccg agggccatgg aggtcaccga gccttacgac agtttgagct
     2101 ttgtgccaac gctgttggca ttaagaggag atttacgcga tgatagcaac ccagttccgg
     2161 tactttggga tagaggtttc aggcgctttc ctggtcgctt gatcaaggag ctattaccgg
     2221 ctaatcagga caaatcaact attgttactg gagcaagcgg atcgccttga ttgaatctcg
     2281 agaagagtta actgagaact ggcctaccga cgagggcgaa gaagtctctg aagaatcagc
     2341 atcgtcgaca tcccgtaatt cacgccgctc cttcgtttgg ctacagacac ttgcgttcat
     2401 cctgggcctt ggcctattgg tttatgtaat ccgtctggtc ggcatacaac cgctgttcga
     2461 cgcgctgctg cgaatcgggt tcggcttctt cgtcatactc ggattaagtg ggttgcgcca
     2521 cgtattacgg accatctcaa tgcgagcggc cgttccgcct gagcatcgtc aaataacatt
     2581 tcgacaagcg tttgccgcaa ggttaggtgg agaggcgata agctttttaa ccttcactgg
     2641 cccactactc ggagaagcca ccaaggtagc actattgcgg aaacgcgtgc cgctcaccta
     2701 tggcgttccg gcactcgtcg tcgataacct tatctacaac ctgtcggtgg tcttttttgt
     2761 gctgagcggc gcctgtgtga tgctgctcag atatccgatg cctacgcccg tttaccttgt
     2821 cttgttggct atcgctttgg ttgcggcact cggaatactg acggcaatct ttgcggcaaa
     2881 acgtagggtc atgttattga cgtggctcat agatcggatg gcgcagctga gattgagccc
     2941 gaaggtcgtg ctcaagaaac gacaacacat ttatcacctg gagtcgaagg tttacgattt
     3001 ttacaaacat catccgggtg cgttctttgg gatgatcgct tgcaatcttt tggcacacgc
     3061 cgccagcgtc gccgaggtct acctggcttt acgttttctc ggatttcagc cgcacgtcgc
     3121 acaggcgtac atcatcgaat ctcttacgaa ggtaattaac ttcgctttcg cattcgtgcc
     3181 gggaaccatt ggcgtttatg aaggcggcac tgaagtaata cttcagaaag ggttaggctt
     3241 tactccagca gctggactcg ctctcgctct agtccgaaaa gcggctattg tttgttggac
     3301 ttcagccgga ttattggtat taacttcgcg gacgctgcca aacgcatggc gtcgaatatt
     3361 ggaccgcagc ccacgtttac aacgacttat ggacagcctc gtattctcta atatcgctca
     3421 tcgacccgct cgaacagccg ttagtattct cggcaccggg cttggtgttt tactaattgt
     3481 cttcactgtc ggcctcgcgc atggagtctt gcacgaacga ggacgtcggg aatcgagcat
     3541 tggagccgaa ataatgattc gcgcttccgg cacgatcagc ctgggtggat catctccgtt
     3601 tcgtttgcct gtttcccacg ccgccgagtt ggcaaaggtc gagggagtaa aggctgcgac
     3661 accaatcggg cagacgctgg accgtagtga ttcaggattc ggttctagat tgattgatgg
     3721 aattgaatat aacgagtacg ccgctctggc aggactcagt atgcgagaag gtcgaggctt
     3781 gatgtccgga gatgaggcca tcgttgatcc cgaatggaaa gagcgtcgta aaacgcagat
     3841 cggtgaaaaa gttaaactgt tcgagcgaca atttacgatt gtcggagtat acgaaccacc
     3901 gggtggtgga cgaatcaaaa ttccgctgag gacccttcag gaacaggaag gtgcagaagg
     3961 gcgagcatct gcaattcttg tctcctgtag tgatccggca aagcaggatg aggttgcggc
     4021 gcggatccta agccagtttc caagtgacca attgatattc actcgtgacc tacctgacat
     4081 ctatgccagc ggtgtgcccg ctttgaatgt gttcatcaaa gtcgtcgtcg gcgtggctgc
     4141 cgcaatcagt atgttggtga tcctattggc gatgtatacc accgttac
//
LOCUS       DASSFP010000450         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1027121, whole genome shotgun sequence.
ACCESSION   DASSFP010000450 DASSFP010000000
VERSION     DASSFP010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027121"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFH91_10110"
     CDS             <1..268
                     /locus_tag="VFH91_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6863395.1"
                     /translation="TIFKHGSLLVEIYAPRGTDPQKPHTRDEVYVVVNGTGEFVCGES
                     RQNFAPSDILFAAAGVIHRFENFTEDLTLWVIFYGPEGGESDAA"
     gene            258..656
                     /locus_tag="VFH91_10115"
     CDS             258..656
                     /locus_tag="VFH91_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863396.1"
                     /translation="MQHDLPTFGAIAPCFPVSDVGATIRWYQLKLGFWSDAFPEAEPY
                     VFAILFRDNIEIMLQRVENYEKPDLYYRRGEGVWDAYIRTTGVKEFWESVRDNVTVVK
                     PLRQQPYGAWEFEVKDLNGYILVFSEIIGS"
     gene            complement(643..2388)
                     /gene="polX"
                     /locus_tag="VFH91_10120"
     CDS             complement(643..2388)
                     /gene="polX"
                     /locus_tag="VFH91_10120"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET6863397.1"
                     /translation="MTKQETPRPDKFAIAAALQEIAALMELKGGQNRFKAKAYNAGAR
                     SIQGVANLEQLIAEDRLTSLPRIGGALASQIKQLYFTGESSVLSGLREEFPAGVVELS
                     AVPGLSLSKIKQLYEELGIKSIAELEAAAEAGQLRNLKGFGAKTEERLLELIRSKSEK
                     PSPRLHLHHAINVANNVIEFLKSNKNVIAVSIAGSLRRWVETVGTIDLVVNTKNPSAV
                     QKHLLRFPLIASSESQDEDSVALQLVDGARVVVTFASPETYALALLVKTGSDKHLQRL
                     QQIATANKITLGVLKGRSKQNNRVVDEEVALYRQLKMQFVPPELREDEGEIEAALAGN
                     LPEDLLTLGDIRGMVHCHTTYSDGKNSLEEMVLAAEAMGMQYITITDHSPTAFYANGL
                     SIDRLERQWEEIDEIQERVKIKILRGTESDIVASGALDYPDRILEKFDVIVASIHSRY
                     KMDKQKMTDRIVTAMRQPVFKVWGHALGRLLERRPPFECDVERILDVIAESKAAVEIN
                     GDPYRLDLEPRWVREARKRKIKFVISVDAHSTGALFNLQYGVRMARRGWVRRGEVLNT
                     LTPTAFAKSVRPTIR"
BASE COUNT          687 a          751 c          668 g          718 t
ORIGIN      
        1 caccatcttc aaacatggaa gcttgctggt cgagatttac gcaccacgcg gcaccgatcc
       61 acaaaagccc cacactcgcg atgaggtcta cgtcgtcgtc aacggtaccg gtgaatttgt
      121 ctgcggagaa tcaagacaga actttgcgcc ttcagacatc ttgtttgccg ccgccggagt
      181 aatacaccgg ttcgagaact ttacagagga cctgactcta tgggtcatct tctatggacc
      241 agagggtggt gaatcagatg cagcatgatc ttccgacctt tggcgcaatt gctccctgct
      301 tcccggtctc agacgtgggc gccacgattc gatggtacca gttaaaactt ggcttttgga
      361 gtgacgcatt tccagaagcc gaaccctacg tttttgctat tttgttccgc gacaacattg
      421 agatcatgtt gcagcgagtt gaaaactacg agaagcctga tctttattac cgccgaggcg
      481 agggcgtttg ggatgcgtac attcgcacca ctggagtcaa agagttctgg gagtctgttc
      541 gcgacaacgt gacggtggta aagccattac gtcaacaacc gtacggcgcc tgggagtttg
      601 aagtgaagga tttgaacggc tacatcctgg tattcagtga aattatcgga tcgtaggcct
      661 tacacttttc gcaaatgctg tcggggtaag tgtgttcagg acttcaccgc gacgcaccca
      721 gccccggcgc gccatcctca ctccgtactg caagttgaac aacgcgccag ttgaatgggc
      781 atcaacggaa ataacaaact taatcttacg cttgcgcgct tcccggaccc atctgggctc
      841 gaggtccaac cgatagggat cgccgttgat ctcaaccgct gctttcgatt cagcaataac
      901 gtccagaatg cgctcaacat cacactcaaa cggaggtcgt cgttcaagca agcgacccaa
      961 agcatgtccc catactttga aaaccggttg tcgcattgcc gtgacaatcc tgtcagtcat
     1021 cttttgcttg tccatcttgt agcgtgaatg gatgctggcg actatgacat caaacttctc
     1081 aaggatccga tcgggatagt ccaaagcgcc gctcgcaacg atgtccgact ccgtgccacg
     1141 caaaatcttt atcttgacgc gttcctgaat ctcatcaatc tcttcccatt gacgctcgag
     1201 ccggtcaatg gacaatccgt tggcgtaaaa cgccgtcggc gagtgatcgg ttatggtgat
     1261 gtactgcatt cccatcgcct cagcagccag taccatctct tccaaactat tctttccatc
     1321 agagtaggta gtgtgacaat ggaccatgcc tctgatgtca ccaagcgtca acaaatcctc
     1381 aggcaagttt cccgctaacg cagcctcgat ctcaccttcg tcttctcgca gctcgggtgg
     1441 cacaaattgc atctttaatt gccgataaag agctacttcc tcatcaacga cccgattatt
     1501 ctgtttactt cttcccttga ggacgccgag agtaatcttg ttggcagtag cgatttgctg
     1561 aagtctctga agatgtttgt ctgagcctgt ttttactaac agcgcgagtg cataagtttc
     1621 aggcgaggcg aacgtaacaa ccacccgtgc gccatcgacc agctgcaacg ccacgctgtc
     1681 ctcgtcctgc gattccgacg acgcaatcaa cggaaatcgt agtaggtgtt tttgtaccgc
     1741 cgagggattt ttggtgttca caaccagatc aattgttccg acagtctcga cccagcggcg
     1801 cagcgaccca gctatgctca ctgcaattac attcttatta gacttcaaaa actcgataac
     1861 attatttgct acgttgatag cgtgatgtag gtggagtcgt gggctcggct tttcggattt
     1921 actgcgaatc agttcgagta aacgctcttc ggtttttgct ccgaatccct tcagattacg
     1981 caattggccg gcctcagctg ctgcctcgag ttcggcaatg gatttaatgc ccaactcttc
     2041 gtagagctgt ttgattttgc tgagactcaa acctggcact gccgatagtt cgacgacgcc
     2101 ggcgggaaat tcctccctta gaccactcag aacagacgat tcaccggtaa agtaaagttg
     2161 cttaatctgc gaagcgagcg cgccacctat gcgtggcaac gacgttagac gatcctcagc
     2221 aatcaattgt tcaagattag caaccccttg aatagaacga gctccagcat tgtaggcttt
     2281 cgccttgaag cgattctggc ctcccttcaa ttccatgagc gccgcgatct cctggagtgc
     2341 cgcggctata gcaaatttgt ctggtcgagg tgtttcctgt ttggtcatga ttgaaattta
     2401 gtttctcttg ttggatacga ttttgcaaaa ggatgcgtca ctctgcgctt ctctgcggtt
     2461 ctctgcgtca ctctgcgtaa ctctgcgttg acctcttata agggcgcttt ggagttaacg
     2521 cagagaaccg cagagttacg cagagaatcg cagagggaac atctaaatga ttggagattg
     2581 tcaatcataa ttctcaattc ccaagcgctt gagagccaaa ctggcaatcc caaagctgta
     2641 taatccctaa gaaatagatc caactggtga acacatatgg cgatagcaac tataaatccg
     2701 accaacggcg agacgttaag aaccttcgcc gcgctcgatc aaaatcaact ggaagaaaaa
     2761 ctacaactag cacatgacac gtatgacgcg tatcgccgca catcgttcga tgaacgcgag
     2821 tcgt
//
LOCUS       DASSFP010000451         9629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1914591, whole genome shotgun sequence.
ACCESSION   DASSFP010000451 DASSFP010000000
VERSION     DASSFP010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914591"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFH91_10125"
     CDS             <1..233
                     /locus_tag="VFH91_10125"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6863398.1"
                     /translation="VYCEMKPGTALFFHANLLHRSDANRSPNPRWSLICCYNAARNPC
                     KAKPNHPAYSKLEKWPDSRIKEIGRRQLGNFS"
     gene            complement(298..1056)
                     /locus_tag="VFH91_10130"
     CDS             complement(298..1056)
                     /locus_tag="VFH91_10130"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HET6863399.1"
                     /translation="MIENLIYPIAKVLLMTITLLFGCLCVLGQDARLPVYEVARVAQP
                     IQIDGRLDEQAWKETTPVGNFVNNRDGTPSNIKTEARLLYSDKFIFFAFRALDSNIWS
                     TFKRRDEHLWLEEVVEVFLQASAQPPNYIELEVNPLGTMLDIYLLDTRKPIPYESWNS
                     RNLRWAVKVDGSVDGREGDREWTCEIALPFEDVVTAPNNPPKSGDRWRMNLFRVERKP
                     AVAEVAWSPTLQDDFHVLPKLGELVFTDRVVPKP"
     gene            complement(1053..2411)
                     /locus_tag="VFH91_10135"
     CDS             complement(1053..2411)
                     /locus_tag="VFH91_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6863400.1"
                     /translation="MEQPHLIRRFGLLEATALNMTNMIGIGPFITIPLLLSALGGPQA
                     MLGWLVALVITISDGMIWSELGAAMPGSGGSYVYLREGYGRETYGRMMGFLFIWQFIL
                     SGPLEIASGYLGFTQYLGYIWKDMNRYQSLAVVVGLGLLNIALLYRKITFIGKLTVSL
                     WVGTILTTFVVIITGAFHFNPGLAFDFPPGAFNFSLGFFFGLGAATRIGIFDYLGYYD
                     ICYIGDEVRQPGRTIPRSIIISVIAVALIYIAINFSVIGVVPWREFVPAEQHPQSSFV
                     VSIFMERIYGGGVATFFTAMVLWTAFGSVFALLLGYSRVPYAAAIDGYFFRVFGKLHP
                     RKNFPYVSLIVIGVIAIICSFFSLGMVIDALITTRILIQFIGQILAVSLLRRNEPGMN
                     RPYRIWLYPIPNLLALFGWIFVFATTNWTIIAFGLGTLVLGLLSFIIWSRQTKQWPFA
                     TT"
     gene            complement(2466..3998)
                     /locus_tag="VFH91_10140"
     CDS             complement(2466..3998)
                     /locus_tag="VFH91_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET6863401.1"
                     /translation="MTLQKATVTCLILLLVHVASGIAVAKTVFVHLLDGDDRAGDGSY
                     SRPFKTWREVLQHVGSGDTIVAKNGDYRKAGREGKWGGLDLILTLEDRLDSDDPHPKL
                     TGRPDTVGIYRYDPQKPLTIRAESKYGVILDHVRFHLASGVVIDGFDIIPNPYYTDDT
                     GRKLNSRRDGIHGDSVYEPDDRYNRVKTNDPPGSYTSQWYDRSLWTSYITVRNCKVHY
                     ECPPSGCTAAYDPMQDDSRLYLIKFNQAHHITIEDNELFDGKNYQRKPAIDLPCSDDV
                     VVRRNLIRNCHRGVVSKGGGSRVLIEANVFVDNSGSPFSGGSTDPDLFIDGKYGDPCS
                     FAVFESYDMTARDNLVVSTRPGEHPVEPISIWAAKNAVITNNTFVGIGERGVLLVRPG
                     NEVDSPGKNCGRSVRLVKTDGLTLTNNIFVLSGVVDETMLYQTSGVGTSLSGFVHRDN
                     TFFNAGRDVPVGGIADPNKEAGFTKDDPKLKGGIGSDYSSWMNTARLDRGSPSSGRGV
                     RN"
     gene            complement(3995..5290)
                     /locus_tag="VFH91_10145"
     CDS             complement(3995..5290)
                     /locus_tag="VFH91_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6863402.1"
                     /translation="MKIYSVLLLLTACVLVSAQPKRSVREVNQVAKPTAGSLIAIVGA
                     TLIDGRGGPAISDSVVVIRDERIAAVGNRSTVKVPQGAEVFEAKGLTLLPGLIDAHFH
                     IDGDDPLPALYLSHGITSLRDPGQWIEAYDVARKGLAPIPRLFLCGPHLDSPPPAYPL
                     DSYVVRDAEETRLAVNRFADDGASAIKVYYRLPVSLIRVAIETAHARGIPVMGHLEIV
                     DARDAIRAGIDGIEHATSFGTALLPLRDAEKYRQSVLADNNARREGRYQVWNSINFDT
                     SQARDLFKLIIDRGVFVSPTLAVFERQQGDKDTTEMHLHGFKQMETFVRLAQQAGAKI
                     VVGSHSDVPHAKRGWAYQRELELLVESGLTPMQALVAGTLQNARYFRIDDRLGTIETG
                     KVADLVMVEGDPLKNISNMRRVKRVMLNGNWVKAESESQ"
     gene            complement(5353..7761)
                     /locus_tag="VFH91_10150"
     CDS             complement(5353..7761)
                     /locus_tag="VFH91_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HET6863403.1"
                     /translation="MKRISKQFYPMATRNEVLMVLPFLLTILLLTPVAFARSPSQPTR
                     PRMLIGDRDPLTGLNVLRARYAAGARPPDDIDGWALTYLLNKDEVFAKRALDKMRTTH
                     PPELVGSRTYPEFIKWSLAFDWLYNYRDFDGPLKDRIAVELLNAAEKMMADQSLKEVQ
                     LAMYHNYPVRYLTLAFFCLTAIEGHPSVETRAAPLRKHARNVIDHILDLTDFITPDGG
                     YHESMDYQRITYAPLAMIAELLRTTANSDPALRYTVFSHYTDTYLYKVLPDGTTARDD
                     DNEFPYLQWEDNICFGYAVNRFKDPYAAWMLRESGWPAQRTWRVPITQFLWDDPDVTP
                     RNPADSTEAEISRNHLFRGIGHLIMRDGFGPNSTWIEFNSGPYLAKHDHLDQNHFVIY
                     HKGYLATESGADYTDTESPHYLNYYRRTIAHNSMLVYQPGEKFFWAENLWPAANDGGQ
                     RMDSSRYWNTVRSREDFERTRDLWATGNMEVTDYQPGVYHYAKGNATRAYQPSKMERF
                     TREVTYTPGNNVVVVFDRVRTTNPDYKKVWLLHGVAEPVVEAPQKFREIGNGGKDYPN
                     ASTFTYLNGEGRLRVHSLLPRERDVVIRGGPNWEFWTPGDEFGGNWGTGKNWPLDPAA
                     GGPLPADPYLRKMWKTFWGEDFEKLLPSNTRAVVPAAWRVEVSPAKPAKENFFLHVME
                     IGDKGDPRSPKVELVDGSNLVGAVIEGGMVTLFATTEEVVSEGEATIPDVETESLLIS
                     GLKPHAKYELQMTGGRANWRGGLFNGVPAGMFIGTANDKGVLFVPFKGQKDGRLRLRM
                     LAAL"
     gene            complement(7841..9175)
                     /locus_tag="VFH91_10155"
     CDS             complement(7841..9175)
                     /locus_tag="VFH91_10155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6863404.1"
                     /translation="MNKRALLVSLVFSLSLGFSATVPAQHKTAVSRQKQVNARLLQLV
                     EANIVGTHSFQVLLAKKSGKGDLTCFGPGKLSDNQLEALVAHQAKLLKADIKAVNAWV
                     RGAGSSFDPGQDLEPLLTSGLAPGPNAPVNVFDNYLQQRTKLGPIKTRAISSLYQTVL
                     EVERDGDLLQDQFAFYIALGLPVYIRQLHLPGTDSDMLSYGRDLVGRSCTAPFDTGAA
                     EWQIAGRKIWNWGQKNLHIRDERVLSAELLQEPDMRALKRALQALPPQKIAVIGHSFT
                     MGLHWSSPSSFVPIVIDVFRRENPRVQFKQFAAGGLTASRAQKRFYQDVLSWKPDKVM
                     LVVMTRADEDYAALKQMGDGFRAAGIRAYMFDEVHDPAAVTPGTVERARKVAEEANIE
                     VIEVGSILASSPDRPHFICLDGIHMTEPYHRLMAKEWLKFLADARTATLSSR"
     gene            complement(9387..>9629)
                     /locus_tag="VFH91_10160"
     CDS             complement(9387..>9629)
                     /locus_tag="VFH91_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6863405.1"
                     /translation="EARALYERLAALNDDRKMLETEPWPPAGAIRFVLAGSALKALGR
                     SADARIALERALKLDPKNELALLQLAELTGDKTGQR"
BASE COUNT         2368 a         2592 c         2347 g         2322 t
ORIGIN      
        1 tggtctattg tgagatgaag ccgggcactg cgctattttt tcatgcaaac ttacttcatc
       61 gctcagacgc caaccgcagt ccaaatcctc gttggtcctt gatctgttgt tacaacgccg
      121 ctcgaaatcc ctgcaaagca aaacctaatc atcctgcata ttccaaactc gaaaagtggc
      181 ccgactcgcg aataaaagag atcgggagga gacagctggg caacttctcg taacctagct
      241 ccattttgcg agaatcgtat agggcctata agtcatatag gtcctatagt tcacccttca
      301 tggtttcggc acgactcgat ccgtaaatac aagttcgcct agttttggca acacgtgaaa
      361 gtcatcctgc aaggttgggg accatgcgac ttcagcgact gcaggcttac gttcgacgcg
      421 aaacagattc attcgccaac gatcacctga ctttggagga ttgtttggcg cggtcaccac
      481 gtcttcgaaa ggaagcgcga tttcacacgt ccactcacga tcgccttctc tgccatcaac
      541 tgagccatca actttgacgg cccatctgag gtttctgctg ttccaacttt cgtaaggtat
      601 cggctttcgt gtgtctaaca aataaatatc gagcatggtc ccaagtggat tcacttcaag
      661 ttcgatatag ttgggcggct gcgcgcttgc ctggagaaag acctcgacga cctcttcgag
      721 ccaaagatgt tcgtcgcgac gcttgaaggt ggaccagatg ttcgagtcta gcgctcgaaa
      781 cgcaaagaaa atgaatttgt cggagtagag taggcgagct tccgtcttaa tatttgaagg
      841 agttccatca cgattattga caaaattacc gaccggcgtt gtttccttcc acgcttgctc
      901 atcgagccgg ccatcgatct ggatcggctg agctactcga gcaacctcgt acacaggaag
      961 tcgcgcatcc tggcccaaca cacagagaca accaaacaga agcgtgattg tcatcagtaa
     1021 aactttggcg atcggataga tcaagttttc aatcatgtgg tagcgaacgg ccactgtttt
     1081 gtttggcgag accaaattat gaacgacaat aaaccgagta ccagcgttcc aagtccaaag
     1141 gcaataatgg tccagttcgt cgtcgcaaag acaaatatcc aaccgaagag cgctaacaag
     1201 ttgggtatgg gatataacca gatccggtag ggccgattca taccgggttc gtttcttctg
     1261 agtaacgaaa cggcaagaat ctgcccaata aactgaatca gaatccgcgt cgttatcaac
     1321 gcgtcgatca ccatgccgag cgaaaagaag ctacaaatga tcgcaataac tccgatcaca
     1381 atgagcgaca cgtagggaaa attctttcgc gggtgtagtt tcccgaacac ccggaagaag
     1441 taaccatcta ttgctgccgc atagggcacc cgtgaatagc caagcaacaa agcgaacacc
     1501 gatccaaatg cggtccacaa caccatcgcg gtaaaaaacg ttgcgacccc tccaccgtaa
     1561 attctttcca tgaaaataga gaccacgaaa ctggattgag gatgttgttc ggccggaaca
     1621 aattcacgcc agggcaccac tcctatcaca gagaaattaa tagcgatgta tatcagcgcc
     1681 acggcgatga cactgatgat gattgagcga ggaattgtac gaccaggctg ccggacttcg
     1741 tcgccgatat aacagatgtc gtaatagcca aggtaatcga atattccgat tcgcgtagca
     1801 gctcccaggc cgaagaaaaa accgagtgaa aaattgaagg cgccgggcgg aaagtcaaaa
     1861 gccagtcccg gattaaaatg aaatgctccc gttatgatca caacaaatgt agttagaatc
     1921 gttcccaccc ataaactgac tgttagtttt ccgataaaag tgatcttccg gtacaacaaa
     1981 gcgatgttca gcaacccgag tccaaccact accgccaatg actgataccg gttcatatct
     2041 ttccagatgt atccgaggta ctgagtaaag cccagatatc ctgaagcaat ctccaatgga
     2101 ccactcaaaa tgaactgcca gatgaagaga aagcccatca ttcgcccgta cgtttcccgc
     2161 ccataacctt ccctcaggta gacatacgag cctccggaac ctggcatcgc cgctccgagt
     2221 tcgctccaaa tcatcccgtc cgagatcgta ataaccagcg caaccagcca accgagcatg
     2281 gcttgtggac caccaagcgc cgagagaagt aacgggattg tgatgaatgg tccaatcccg
     2341 atcatgttgg tcatgttcaa ggccgtggcc tcgagcagtc caaaccggcg tattaagtgt
     2401 ggttgttcca tctttggagt gcgccggccc gtcggcgctt tgttaatcta ttccctccca
     2461 aactttcaat tccgaactcc cctaccactt gacggtgacc cccggtccaa cctcgctgtg
     2521 ttcatccacg aactgtaatc actgccaatt ccacccttga gttttggatc atctttagtg
     2581 aagcccgcct ccttatttgg atccgcaatt ccaccaactg gaacgtcacg acccgcatta
     2641 aagaatgtat tgtcccgatg aacgaaaccc gataacgacg ttcctacccc ggaggtttga
     2701 taaagcatcg tttcgtcgac gaccccagac agaacaaaaa tattgttcgt gagagttaac
     2761 ccatcagtct ttactaagcg aaccgagcgc ccacaattct ttccgggaga atcaacttca
     2821 ttaccggggc ggaccagcaa aaccccgcgc tcgcctatac ccacaaacgt gttattagtg
     2881 atgactgcat tcttcgcggc ccagattgaa atcggttcga ccggatgttc tcctggacgg
     2941 gttgatacga ccagattgtc ccgcgcagtc atgtcgtagg attcaaaaac ggcaaaggaa
     3001 cacggatcgc cgtattttcc atctatgaac agatctggat ccgtggagcc gccggaaaac
     3061 ggtgagccgg aattatcgac gaacacattt gcttcgataa ggactcgact gcctccacct
     3121 tttgaaacga ccccgcgatg acagttgcga atcaggttgc gccgaaccac gacgtcatcg
     3181 ctacaaggaa gatctatagc gggtttgcgt tggtaattct ttccatcaaa tagttcgtta
     3241 tcttcgattg tgatgtgatg agcttgattg aacttgatca gatacagccg tgagtcgtcc
     3301 tgcattgggt cgtacgcagc ggtacatccg ctcggcgggc attcgtaatg gaccttgcaa
     3361 tttcggacag taatatagct ggtccataac gaacggtcat accattgcga agtataacta
     3421 ccaggtggat cgttggtctt caccctgtta tatcgatcat caggctcata gacgctgtcg
     3481 ccgtgaatac cgtcccggcg tgagtttagc tttcgacctg tgtcgtccgt gtaatatgga
     3541 tttgggatga tgtcaaaacc gtcaattacg acgccgctgg caagatgaaa tcgaacgtgg
     3601 tctaagatga ctccgtattt cgattcagcc cgaatcgtta aaggcttctg cggatcgtag
     3661 cgatagatac caactgtatc aggacgtccc gtgagtttcg gatgtggatc atcactgtcc
     3721 agtcgatctt caagagttaa gattaaatcc aggcctcccc atttaccctc gcgacctgct
     3781 ttgcgatagt caccattctt cgcaacaatc gtatctccgc tacctacatg ctggagcact
     3841 tctcgccatg ttttgaaagg acggctgtag cttccatcac cggcacggtc gtcaccatcg
     3901 agtagatgga caaacaccgt cttcgcaaca gcaattccgg atgcgacatg gaccagtaac
     3961 aagatgagac aagtgacagt cgctttctga agagtcattg gctctccgat tctgctttga
     4021 cccagttgcc gtttagcatc actcgtttta ctcggcgcat gttgctgatg tttttgagcg
     4081 ggtcgccttc gaccatcaca aggtctgcca ctttgccggt ttcgattgtt cccagcctgt
     4141 catcgatgcg aaagtagcgc gcattttgca acgtgccggc gaccagagct tgcattggcg
     4201 tcagacctga ctcgacgagc aactcgagtt cacgctgata ggcccatcct ctttttgcat
     4261 gcggcacatc cgaatgcgaa cctacgacga ttttcgctcc ggcttgttgg gcaagcctca
     4321 caaatgtttc catctgtttg aaaccgtgca ggtgcatctc agtcgtatct ttatcaccct
     4381 gttgtctctc aaaaaccgct agggtaggtg agacgaaaac cccacgatct ataatcagct
     4441 tgaagaggtc gcgggcctgc gaagtgtcga aattgatcga gttccacacc tgataacgac
     4501 cttcgcggcg cgcgttgtta tcggccagaa ctgactggcg atacttctcc gcgtcacgta
     4561 gcggcagtag agctgttcca aatgatgtgg cgtgctcgat gccgtcgatt cccgcccgga
     4621 ttgcatctct ggcatcgacg atctccaggt ggcccatcac tggaatgcct cgcgcatgag
     4681 ctgtttcgat cgccactcga ataagcgaga ctggtaatcg gtagtaaact tttattgccg
     4741 atgctccatc atctgcaaaa cgattcactg caagacgcgt ttcctcagca tctcgaacta
     4801 cgtaggaatc cagcggatag gcgggcggag gtgagtcgag gtgtggtcca caaaggaaca
     4861 ggcgcggaat tggtgcgaga ccttttctcg caacatcata ggcttcgatc cactgaccag
     4921 gatcgcgaag agacgtgatg ccatgagaga ggtaaagggc aggtaaagga tcatcgccgt
     4981 cgatgtggaa atgggcgtcg atcagtcccg gcaataacgt cagacccttt gcttcaaaga
     5041 cttctgcacc ttgcgggact tttacagttg atcggttgcc aactgcagcg atgcgttcgt
     5101 cgcggataac aacgaccgaa tcagagatcg ccggtccgcc acgtccatcg atcaaagtgg
     5161 caccaacgat agcaattaat gaaccagctg tcggtttggc aacctggttg acctcacgta
     5221 cagatcgttt tggttgtgca cttaccagaa cacaggctgt cagtaaaagt aagacgctat
     5281 aaattttcat atcgaacttt gttctctctg cgtctcagcg ccggaagctt ttcttcccac
     5341 cgcagaggct atttacagtg cggcgagcat gcgaagccga agcctcccat ccttctgacc
     5401 tttgaaaggc acaaacaaga cgcctttatc atttgccgtt ccgatgaaca tcccagccgg
     5461 gacaccgttg aacagtccac cacgccagtt tgctcggcca cccgtcattt gcagttcgta
     5521 cttcgcgtgc ggcttcagac cgctgatgag taagctttca gtctcaacat caggaatcgt
     5581 tgcctcacct tcactaacca cctcctcggt tgttgcaaat agggtgacca tccctccctc
     5641 gatcaccgcc ccgacaagat ttgagccatc aaccaattca actttcggtg agcgcgggtc
     5701 acctttgtct cctatctcca tcacatgaag aaagaagttc tcctttgccg gttttgccgg
     5761 agaaacctca actcgccacg ccgccggaac aactgcgcgg gtgttcgacg gcaggagctt
     5821 ttcaaaatcc tctccccaaa acgtcttcca catcttccgc aggtacggat ctgcaggcag
     5881 tggaccacca gcagcaggat cgagtggcca attttttccg gttccccaat taccgccaaa
     5941 ctcgtcgccg ggtgtccaga attcccaatt cggacctcct ctaatcacaa cgtcccgctc
     6001 tcgtggcagc agcgaatgga cacgcaacct tccttctcca ttcaggtaag taaatgtcga
     6061 ggcgttcgga taatcctttc cgccattacc tatctcgcgg aatttctgcg gcgcttctac
     6121 taccggctcg gctaccccgt gaagtagcca cacctttttg taatcagggt tggtggtccg
     6181 aacccgatcg aatacaacca ccacattgtt gcccggcgta tatgttactt ccctggtgaa
     6241 gcgctccatc tttgatggct gataggcccg ggtagcatta cctttcgcgt agtgatagac
     6301 gcccggctga taatcagtga cctccatgtt gccggtggcc cagagatctc gtgtgcgctc
     6361 aaagtcttcc cggctacgaa ccgtgttcca gtagcgcgag gaatccatcc tttgaccgcc
     6421 atcgttagct gcgggccata gattctcggc ccaaaaaaac ttttcacctg gttggtagac
     6481 aagcatcgaa ttgtgagcaa ttgttcgccg atagtagttg agatagtgag gactttctgt
     6541 gtccgtgtag tcggctccgc tctcagttgc gagatagccc ttgtgataaa tgacgaaatg
     6601 attctggtcc agatgatcgt gctttgccag atacggacca gagttgaact cgatccaggt
     6661 ggagtttggt ccaaagccat cgcgcatgat cagatggccg atgccgcgaa atagatgatt
     6721 gcgagaaatc tcagcttcgg ttgagtcggc agggttgcgc ggtgtgacgt caggatcgtc
     6781 ccagagaaac tgcgtgatcg gaacgcgcca cgtacgctgc gcaggccatc cgctctctcg
     6841 taacatccag gcggcatacg gatccttgaa tcgattgact gcatagccaa aacaaatatt
     6901 gtcttcccat tgaagatacg ggaactcatt atcgtcatct ctcgcggtag tgccatccgg
     6961 tagcacctta taaagatacg tatcggtgta atgactaaag accgtgtatc tgagggccgg
     7021 gtcgctatta gcagtcgttc ttaaaagctc tgcgatcatt gccagcggtg cgtaagtgat
     7081 acgctgataa tccatcgact cgtgataacc gccatctgga gtgatgaagt cagtcagatc
     7141 gaggatgtga tcgatcacgt tacgcgcatg ctttcttaaa ggcgctgcac gtgtctcaac
     7201 tgaaggatgg ccctcgattg cggtaaggca aaaaaatgcc agcgtcaggt aacgaaccgg
     7261 atagttgtga tacatcgcga gttgcacttc tttcagcgac tgatctgcca tcatcttttc
     7321 ggcagcattc agtaattcaa ctgcgatgcg gtccttcaac ggaccgtcga aatccctgta
     7381 gttgtagagc caatcaaaag cgagtgacca cttgataaac tcaggataag tgcgagatcc
     7441 gaccaactct ggtggatgcg ttgtcctcat cttgtcaagg gcacgtttcg caaaaacctc
     7501 gtccttattt aatagatagg tcagcgccca tccatcgata tcgtcagggg gccgggcgcc
     7561 tgccgcatat ctcgcgcgca agacattgag gccggtgaga gggtcacggt cgcctatcaa
     7621 catacggggt ctggtaggtt gagatggcga tcgagcgaat gcgactggag ttaataaaag
     7681 aatggttagc aaaaacggca gaaccatcag cacttcattt cgcgttgcca tcgggtagaa
     7741 ctgcttactg attcttttca cgtatgactc ccggctttct cagcgtttcg gcgtcttccg
     7801 gcaaaatttc aaccgcaaga cgctgagacg cagactgttt ctatcgactg ctaagcgtcg
     7861 ccgttcgtgc gtccgcaaga aatttcagcc attccttcgc cattaaacgg tgataaggct
     7921 cagtcatatg aatgccgtca agacaaataa agtgcggccg atccggagac gaggccagga
     7981 tggaaccaac ctcgatcact tcgatgtttg cctcttcagc aacttttcgc gcgcgctcga
     8041 ctgttcccgg tgttacggcc gctggatcgt ggacctcgtc aaacatgtac gctctgatcc
     8101 cggctgctcg gaaaccgtct cccatctgct tgagcgccgc gtagtcttcg tcggcgcgag
     8161 tcataacaac cagcattact ttgtcaggtt tccaactgag cacgtcctga tagaaacgct
     8221 tctgagcgcg cgacgcggtt agaccaccgg ccgcaaattg tttgaactgc actcgcggat
     8281 tttcccggcg aaatacgtcg atcacgatcg gaacaaaact agatggtgag gaccaatgga
     8341 gccccatcgt gaatgagtga ccgatgacgg caattttttg tgggggcaaa gcctgcaacg
     8401 cccgtttgag agctcgcatg tctggttcct gcaacagttc ggccgaaagt acacgttcat
     8461 cgcgaatgtg caagttcttc tggccccagt tccagatctt tcgaccagca atctgccatt
     8521 ccgccgcgcc tgtatcaaag ggcgcggtgc acgaacgacc caccagatca cgtccatacg
     8581 agagcatgtc ggaatcggtc ccaggcaaat gcagttgtcg tatgtagacc ggcaaaccca
     8641 gagcaatgta gaaagcaaac tggtcttgca gtagatcgcc gtctcgttcg acctccagaa
     8701 ctgtctgata aagactcgaa atagctcgag tcttaattgg tccaagtttc gttcgctgtt
     8761 gcaggtagtt gtcaaagaca ttgacaggag cattagggcc tggcgcgaga cccgatgtaa
     8821 gcaaaggttc aagatcctgt ccgggatcaa agcttgaacc tgctcctctg acccaggcgt
     8881 ttaccgcttt tatatcagct ttgagaagct ttgcctgatg agcaacgagg gcttcgagct
     8941 ggttgtcaga gagtttccct ggcccaaaac aggtgagatc gcctttgccc gatttttttg
     9001 ccagcaatac ctgaaaggag tgggtgccta cgatgttcgc ctcgactagt tgcaatagcc
     9061 gagcattcac ctgtttctgc cgactaacag cagtcttgtg ctgagcggga actgtcgcgc
     9121 taaagcccag actaagcgag aatacaagcg acactaataa ggctcgctta ttcatttccg
     9181 atctcctgtt cgcatttctt ctctgagcat tttggagtgc gccggcccgg cggcgctttt
     9241 aaagcctagc gcggcgtcat acctcgattt aatttgggaa cttccatttg agagagagct
     9301 atctatatct agagactcct tctcagagat ttcttcgtag agacttcgat ataacaaagc
     9361 gacgccgggc cgtcgcactc caaggttcag cgttgccctg tcttatcacc tgtcaattcg
     9421 gctagctgga gcagggccaa ttcattcttt ggatccaatt tgagcgctcg ttccaaggca
     9481 atccgagcgt cagcactgcg accaagcgcc ttcaaagcag agcccgcgag aacaaatcga
     9541 atcgcgcctg ctggaggcca gggttcggtc tccagcatct tcctgtcatc gtttagtgcg
     9601 gcgaggcgct cgtaaagtgc tcgagcttc
//
LOCUS       DASSFP010000452         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1168088, whole genome shotgun sequence.
ACCESSION   DASSFP010000452 DASSFP010000000
VERSION     DASSFP010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168088"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..639
                     /locus_tag="VFH91_10165"
     CDS             313..639
                     /locus_tag="VFH91_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6863406.1"
                     /translation="MSSRVTRKLRRESKAKMDAYEAVFTALAHPARRRIMLTINFEGG
                     SMTAGQIADVFEHAWPTTTRHIQVLESAGLLYHEQKGRVRVYYINRSRLDLVRDWLEW
                     FAKDPH"
     gene            663..1145
                     /locus_tag="VFH91_10170"
     CDS             663..1145
                     /locus_tag="VFH91_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6863407.1"
                     /translation="MATKKRTKAKTSIKKKVAKKSSTRSKKEVRMDVPSLFRLNIEVG
                     DLDQAAEFYGKLFGLEGRKQAGSRCYFNCGGVTLQVVDVSSSGKPHPAAKALYFLVKD
                     LDSVFERAKALGCLSGEDVHGVSGGAISVRPWGERSFYAEDKWRNPLCFVEEGTVYPG
                     "
     gene            1168..1965
                     /locus_tag="VFH91_10175"
     CDS             1168..1965
                     /locus_tag="VFH91_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HET6863408.1"
                     /translation="MTLPFLKAKPVIGVIHVGALPGTPRSSSKVSQLVDAARIEANIY
                     RDAGIDGLIIENMHDVPYLRGEVGPEVVAAMTAIGSEVKSASGLPVGIQILAGANIEA
                     MAVAHAAGLDFIRAEGYAYAHIADEGFIQASAAKLLRYRRLIGAENVQVWTDIKKKHS
                     AHAITADVSLGETAETVEFMGADAVIVTGNATGRPPNSEDVKEAKANCGLPVLLGSGI
                     SEENISHFYEDADGFIIGTSFKVDGLWSNTVDSSRVESFLKTMRRLG"
     gene            complement(1989..2600)
                     /locus_tag="VFH91_10180"
     CDS             complement(1989..2600)
                     /locus_tag="VFH91_10180"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6863409.1"
                     /translation="MSIVLIIARTQLDVSGRYGNAVTMLAKFLNHQSDNKGEEMRRLY
                     SGFLGGFPGIGLLIIRLAVGAAFIIHGWPKVHNPMGWMNSIWGSPVPSLLQALAALTE
                     FGGGIALILGLLTPLVSLALVVQMVVALAMVHLPAGQHFIATQAGEPSFEIPVIYLAI
                     ALALIALGPGKLSVDWLLFGSPGSARTYRAPVSGRRETSLSYR"
     gene            2878..3255
                     /locus_tag="VFH91_10185"
     CDS             2878..3255
                     /locus_tag="VFH91_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863410.1"
                     /translation="MPAQADFDSTFEQLKKIFEPYRSRLIVSADNSSLFTLEVDHVMK
                     NKHRLYFGGVRRGKAYVSFHLMPVYACPEVQSQISPELKKRMQGKSCFNFSYVDAKLF
                     KELAKLTKAGFKRFTNKEFYSQF"
     gene            3269..4423
                     /locus_tag="VFH91_10190"
     CDS             3269..4423
                     /locus_tag="VFH91_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863411.1"
                     /translation="MPRTFDCPKCGAPVNYGGQAGPEGFTTKCNYCGSMIAEPSMGRP
                     AHVVHVRLDGVSPNIKMSKKVLLILILVPVFAVLGVVIAMIVGFSSALHTVSKPPIVS
                     GRPAREEPKGGLQPGKSLATVLLKFGSKGIGPGMFNDARSIAVDTAGNIYVGEYSNGR
                     IQVFDGTGKFITQWTADPKMPVRGLAADRKGTVYVVQSGKIQKYEGLTGNAIGEVQFS
                     GGWGFDDIAPTASGGLVCAWYKNRDDIVVLDANGKVLKTIQGAISSNSDNSELDTRVA
                     TDGLGNIYALGTFNNAVFKFGPDGKYINRFGGDGDQPGQFRAPQSIAVDGRGRVYVSD
                     IKGIQRFDSDGRYLDTFRADGSSASGMVFNDKNELFVVAREKVIKVGLKD"
     gene            4765..5271
                     /locus_tag="VFH91_10195"
     CDS             4765..5271
                     /locus_tag="VFH91_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6863412.1"
                     /translation="MTPSHREFTDDHTPIAFFITFRTYGTWLHGDERGSVDRFHNRYG
                     TPRLPANKLRQQYEFKLLRQPPVLLTPTQRQVVTETIKKTCKERNWGLWAVNVRTNHV
                     HTVVTANCNSKKVRATLKAKATKAMRETGCWRSDLSPWARRGSRQNLWTYQDLIAAIE
                     YVLYEQGE"
     gene            complement(5681..>6247)
                     /locus_tag="VFH91_10200"
     CDS             complement(5681..>6247)
                     /locus_tag="VFH91_10200"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6863413.1"
                     /translation="NTPSHSNEPSDIRRQPTAPVDTPQLTPDTVSSAPNPNLPVRGDR
                     WRIGNTDSNFQPDGPGSSTHGTGSGNGSAVRVNIPDEPPPAVPTQTPPVKILRISTVI
                     NNKAISLPKPNYPALAKQIKLQGIVNVQVLIDERGNVISAKAVSGHPFLIPEAQRAAI
                     QAKFSPAFIGEQPVKVSGVISYNFVLSN"
BASE COUNT         1611 a         1467 c         1670 g         1499 t
ORIGIN      
        1 cctggtaaaa gtgaaaggtg tggagggtaa atggaagctt tattttccat ttgtcatttc
       61 tcatttttca tttttcattt aaatcgggct ggggcaggca ggaatcattg gagaattatg
      121 aattgatatt tgagaattgc tattttggtc tatggaagcg ttttggatca tgagagcgtt
      181 tgattatgag agcgcccgtg aatccgaaat agcaattctc aaatatcaaa tcataattct
      241 ccaatgactc cctcttattt agcccgtacg ctaaatctcc gcttgacatt aattagccta
      301 cacgctaaat atgtgagttc tagagtgacg cggaaactga gacgagagtc caaggcaaag
      361 atggatgcgt acgaggctgt cttcacagcg ctcgctcatc cagcacgccg ccggatcatg
      421 ctgacgataa actttgaggg cggatccatg acggccggtc aaattgccga tgtatttgag
      481 cacgcatggc ccaccacaac caggcacatc caggtgctcg aatccgccgg gttgctttac
      541 cacgagcaga aggggcgtgt gcgcgtctat tacatcaatc gcagcaggct ggatctcgtg
      601 cgcgactggt tggaatggtt tgctaaagat cctcattaaa gaaaatattt ccggagaaaa
      661 ttatggcgac aaagaagaga acgaaggcga aaacgtcgat caagaagaaa gtagcgaaga
      721 agtcttcaac tcgctcgaag aaagaagtca ggatggatgt gccaagtctg ttccgactca
      781 atatcgaagt aggtgacctg gaccaggcgg ctgagttcta tggcaaattg tttggcctcg
      841 agggacgtaa gcaggcgggc tctcgttgtt atttcaattg cggaggggtg acgctgcagg
      901 ttgttgacgt ctcttcctca ggaaaacctc atccggcggc caaggcactg tattttctgg
      961 ttaaagatct cgactcggtg tttgaacggg caaaggcgct cggctgtctc tccggtgaag
     1021 atgtacatgg cgtctctgga ggtgcaatca gcgtgcgccc gtggggtgag cgctcgtttt
     1081 atgccgaaga taagtggcga aatccgctct gttttgttga ggaaggcacc gtgtatccgg
     1141 gctaagcaca gcctccaatg ggtaaatatg accttaccat ttcttaaagc gaagcctgtt
     1201 atcggggtga ttcacgtagg ggcgctgccg ggtacgccgc gaagcagcag taaggtcagc
     1261 cagctggtcg atgccgcacg gattgaagcg aacatctacc gagacgctgg tatcgatggt
     1321 ctaattattg aaaacatgca cgacgttccc tatctgcggg gcgaagttgg accagaggtt
     1381 gttgcggcta tgactgccat cggtagtgag gttaagagcg ccagtggttt gcctgtaggc
     1441 atacagattc tagctggagc aaacatcgag gcaatggccg tagcgcacgc cgccgggttg
     1501 gacttcattc gcgccgaagg ttatgcgtat gcacatattg ccgacgaagg attcattcaa
     1561 gcttcggcgg caaaactcct acgctatcgt cgcctgattg gagctgaaaa tgttcaagtg
     1621 tggactgata tcaagaagaa acactcggcc catgcgatta ctgctgatgt aagtttgggt
     1681 gaaactgctg agactgttga gttcatgggc gccgacgcgg tgattgttac gggaaacgca
     1741 actggccgcc cacctaatag tgaagatgta aaagaggcaa aagctaattg cggtttgcct
     1801 gtcctcttag gttcggggat cagtgaggaa aatatttcgc atttctacga ggatgcggac
     1861 ggttttataa tcggcacatc ttttaaagtt gacggacttt ggtccaatac ggttgattcg
     1921 tcgcgtgtcg agagttttct caagaccatg cgtcgacttg gttaacaacc tgagcacagg
     1981 caactcactt acctgtacga cagggatgtt tctcgacggc cgcttactgg tgctctgtac
     2041 gtgcgagctg atccaggcga gccaaacaat agccaatcca cagaaagctt tccagggcca
     2101 agcgctatta gcgctaaggc aatcgccaga taaatcacag gaatttcgaa actaggttcg
     2161 ccagcctgcg tcgcgataaa gtgctggccg gctggaaggt ggaccatcgc cagcgccaca
     2221 accatctgca caaccaatgc aagggacaca agaggcgtaa gcagacctaa gatcaatgca
     2281 atgcctccgc cgaattcagt caaggctgca agtgcttgca gcaacgatgg cactggcgat
     2341 ccccagattg aattcatcca gcccatcggg ttatgaactt tgggccaacc gtgaatgata
     2401 aaagcagctc caactgcaag tctgattatt aagagtccaa taccagggaa tcctcccagg
     2461 aatccagaat acaaacgtcg catctcttcc cccttattgt ccgactggtg gtttagaaac
     2521 tttgcaagca tcgtgaccgc attgccatat cgacccgaca catccaactg tgtcctcgca
     2581 ataatcagaa caatactcac gatcgtcagt catccgacac ctgcacgcgg agtgttcgca
     2641 actctttcca tatcgaccgt cccctgcgca ccaaatcaaa acgaccgtgt ttacttccgg
     2701 tacgagtcca gatgcataat gagcctgata ccatgacgat ggcaaacgtc gtaccgccgt
     2761 ccaagaaaat cgccgtaaca tttatgaatt tgttaagcta aacgtcgact cagcggctgc
     2821 gcagcgctgc gttaaacttc aaccttagca gcgtcgcaac gctgattatt aaacacaatg
     2881 ccggcccaag cagacttcga cagcacattc gaacagctaa aaaagatttt cgagccttac
     2941 agatcgcgcc tgattgtgtc tgctgataac agctcgttat tcacactgga agttgatcac
     3001 gtaatgaaga acaagcaccg gctgtacttc ggtggcgtca ggaggggaaa agcttacgtc
     3061 agctttcatc taatgccagt gtatgcatgc ccggaagttc agagccaaat ttcgccggag
     3121 ctgaaaaagc gcatgcaggg taagtcgtgt ttcaacttct cgtacgtgga cgcaaagcta
     3181 tttaaggagc ttgctaaact gactaaggca ggctttaaga gatttacaaa taaagaattc
     3241 tattcgcagt tttaaggagt caacacgagt gccgcgaacc tttgattgtc caaaatgtgg
     3301 agcgccggta aattacggcg gacaggcagg tccggaaggc ttcaccacaa agtgtaacta
     3361 ctgtggcagc atgatcgcgg aaccttcgat gggacgtcca gcccatgtgg tccatgtcag
     3421 acttgatggc gtaagtccaa acatcaagat gtcgaagaag gttttgttga ttctcatact
     3481 cgttcccgta tttgcagtac taggcgtggt gattgccatg atagtcggtt tttcaagcgc
     3541 gctgcatact gtgtcgaagc ctccaatcgt ttctggacga ccagctcgcg aagagccaaa
     3601 aggcggcctg caaccgggca agagtttagc gacggtcctg ctgaagtttg gctcaaaagg
     3661 aattggacca ggaatgttca atgatgctcg cagcattgct gttgatacag ccgggaatat
     3721 ctacgtcggt gagtatagca acggtcgaat acaggtgttc gacggaacag gaaaatttat
     3781 tacccaatgg accgccgatc cgaagatgcc tgtccgcggt ttggcggccg atcgcaaagg
     3841 cacggtctac gtcgtacaga gtggaaagat tcagaagtat gagggactaa ccggaaacgc
     3901 aataggcgag gtccaattct ccggtggctg gggtttcgat gatattgctc ctactgcgag
     3961 tggcggtctg gtttgtgcat ggtacaagaa ccgcgacgac attgtggtgc tcgatgccaa
     4021 tggcaaagtg cttaagacta ttcaaggggc aatcagttcg aacagtgaca actctgaatt
     4081 ggacactcga gttgccaccg acggactcgg aaacatttat gcgctgggta cctttaacaa
     4141 tgcagtgttc aagtttgggc cggacggcaa atacatcaat cgctttggag gagatggaga
     4201 tcagccgggc cagtttcgtg cgccgcaatc gattgcggtg gacggtcgtg ggcgagttta
     4261 tgtaagtgac ataaaaggta ttcagagatt cgactcagat ggtcgctacc tggatacgtt
     4321 cagggccgac ggaagctcag cctcgggcat ggtcttcaac gacaagaatg aattatttgt
     4381 agttgctcgc gagaaagtga ttaaggtggg gttaaaagac tgaggtggag tgaagttgcc
     4441 aacccgccca aactcgaacc tggacgcatt acagaaccac aagcgcagcg cctgttcgca
     4501 cttcccaaac tcgaaccgcc cccattacag aacaagcgca gcgcctgtac acacctccta
     4561 acagcgcgga actcgcgcca ttacagaacc ataagcgaag cgactgtcca cacctcctct
     4621 agcgcgactc gcccgattac agaacgatag cgaagcgact gaacacacct gccaaactca
     4681 tttctaacca atgacaagaa ctcgtcaaat tgttagatga tgaactatta tcatcaccta
     4741 caacctcacg aaaggattaa gatcatgact ccatctcaca gggagtttac tgatgaccat
     4801 actccaattg ctttcttcat tacttttcga acctatggca cctggctaca cggtgatgag
     4861 cggggatcag tagaccggtt tcacaatcgt tatggaacgc cccgcttacc agcgaataag
     4921 ttgcgtcagc agtatgagtt caaattactt aggcagccgc cagtgctttt gacaccaact
     4981 cagcgccaag tggttacgga aacaatcaag aaaacctgta aggagcgaaa ctgggggctg
     5041 tgggccgtca atgtgcggac gaatcatgtt cacacagttg ttacagccaa ctgcaactcc
     5101 aagaaggtga gagccacatt aaaagcaaag gcaaccaaag cgatgagaga gaccggctgt
     5161 tggcgcagtg atctcagtcc gtgggctcgt cgcggcagtc ggcagaatct ctggacatac
     5221 caggatttga ttgcggcaat tgagtatgtc ctttatgagc aaggtgagtg atacgtgtgc
     5281 ccacgcactg aacgcggcaa aaacagatac actcgataga atcttaagtg attcgggcgc
     5341 tgatttgacg tgtgaacagg cgctgcgctt ggggagttct gtttggcaga tgtggacagg
     5401 cgcttcgcct gtggttctgc aatgggggtt cgagtttggg aggtgtggac agtcgctgcg
     5461 cttgtggttc tgtaatgggg taggttcgag tttggttaag tgtgttcagt cgcagcgctt
     5521 accgctcaga aatggaaaat gagaatggaa aataaagatc tcatcaaact caacaattcg
     5581 ctcagaacct accagtgctt ggagggagag gtacgtagga ccggaccaac gtacctcctt
     5641 gctggggtgg gaaaactgaa gttgaattca ctctgccgtg ctaattgctt agcacgaagt
     5701 tgtagctgat tactcccgag accttcactg gttgttcccc gataaaagct ggagagaatt
     5761 ttgcctgtat tgcagctcgt tgcgcttcag gtattagaaa cggatgtcct gaaacagctt
     5821 ttgccgagat cacgttccca cgttcatcga tcaacacctg aacattcacg attccctgca
     5881 gcttgatctg tttagccaac gctggatagt ttggtttggg tagtgatatc gccttgttat
     5941 ttattacggt tgaaatacgc aggattttta ccgggggagt ttgggttgga actgccggtg
     6001 gtggttcgtc gggtatattc actcgcaccg cagatccgtt tccggatcca gtgccgtgag
     6061 tcgagctgcc aggtccgtcg ggctgaaaat ttgaatcagt gtttccaatt ctccacctgt
     6121 cacctcgcac cggtagattt ggattgggcg ccgatgagac cgtgtccggc gtaagttgag
     6181 gcgtatcaac tggggccgtc ggttgacgcc gaatgtcact tggttcattg ctgtgtgatg
     6241 gtgtgtt
//
LOCUS       DASSFP010000453         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1324502, whole genome shotgun sequence.
ACCESSION   DASSFP010000453 DASSFP010000000
VERSION     DASSFP010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324502"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VFH91_10205"
     CDS             <1..1351
                     /locus_tag="VFH91_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863414.1"
                     /translation="IQDLVQFNDPVFAAANNDPRYRVKPIPGRDSDNFEPRLGFNWNP
                     RTSSNGFLGWITGGDKLVVRGGYTRTHDYAYTNIALNIWSSFPFVAAFNLTNVPNAFA
                     NYKTLPVDPAIFTRTQVTDDFHSPTYDSFSFEFQREITRDLVFRVGYVGSKGTGLFES
                     IDGNPVRFRTAVPSAANPAIRTDPTTGPIRLRANSGSSIYHSMQASMEKRLSSGFSAG
                     VHFTWSSFIDSMSEIFNNSNSEIALAQDPYNRALDRGRSSYDRPLRLAGNFVYELPIY
                     RDQQGFVGRLLGGWQLNSNFNFQSGAPFSPLNPSDLSGTLGGLASAIGIATRPNVATN
                     INMSGMSVEDLFALRGTVNASGNALFRALQPGQRIGDAGRNILRSDGLNNVDFGLLKN
                     TRIGENQRLQIRADFFNATNTRDFGTPNSVVTSTGFLRQWDTNGGNRRIIVGIRYVF"
     gene            1522..2136
                     /locus_tag="VFH91_10210"
     CDS             1522..2136
                     /locus_tag="VFH91_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863415.1"
                     /translation="MKYLILPLLFVLTAISSLAQSSKESASAPGLDIIRFSWSKERIG
                     WQRDPFSGPIENFQEVQARTRNERRVQDAKRGGGPEVDKIKTEAKADAANIATQHKTV
                     PARYVFMYKLTVKNSSSKTIKSIDWDYVFLDRATGSELGRREFTSVEKVSPGKSKELT
                     VVISKPPTQTVSLTALNSNERDAMLELVSLVRIEYTDGTAWQRP"
     gene            2152..2601
                     /locus_tag="VFH91_10215"
     CDS             2152..2601
                     /locus_tag="VFH91_10215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6863416.1"
                     /translation="MAIRAATVDEAEELTAIAMAAKRHWGYPENWIKQWEADLTITSD
                     FVSDNTVFVAEEDGEARGFYALCVNDGKAELEHMWVKPQYIVTGVGKELFLHAMDLAT
                     TLKLSELSITADPNAASFYEHMGAERVGEVGSSIEGRKLPRLKIERE"
     gene            complement(2612..4456)
                     /locus_tag="VFH91_10220"
     CDS             complement(2612..4456)
                     /locus_tag="VFH91_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863417.1"
                     /translation="MRSSTIQIIAPAGPIIAGTSLARVLHTSQLTLNSSAGTNEQFVD
                     RTKDLIADDRTTFDSDGGSFDIAVGQSGARYEVYSAIDGNTLLGVLVVATDSNGDFVA
                     NTSEVFDLERDFDLPSAAAVVSGLSSNGREFVIVSSSGYFNSDDPNDPNNEPSPGVIL
                     LVRNSATGGFDESLSRTLVTVGDNKLFNANALALMPNNDLLIADFASNELRIVRDTNG
                     DRIPDTLDTQPYYSYQFSDDAPLDIAVNSRGVVFSHSEGNDTILLAIYDDNSDGRADH
                     DEVVVEGLSIDNNLFLHGLTIDRLGNVYVIEDATSSEDGTGGNGGIARVDAFPDPNLD
                     GFLDDGKVFAQVDGSQFGFSGIGLGPNRLDDTSFFVRQHYLDFLNREPDATGFPFWVN
                     NIDLCGVDVHCREVKRIDTSAAFFLSIEFQQTGFLVHRLYKASFPASAQRPRGLPRFQ
                     EFLADSGTIGNGVIVGASGWEAKLAANTTAFVAAFVSRAEFQTSFPLQMTAAQYVDKL
                     NTQTGGALSTTERNNLVAGLSGGQETRATVLLKVANDVDFTNAELNKAFVLMQYFGYL
                     RRNPDDAPDLNFSGYDFWLTKLNQFNGNFRAADMVKAFLVSREYIERF"
     gene            4861..5106
                     /locus_tag="VFH91_10225"
     CDS             4861..5106
                     /locus_tag="VFH91_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863418.1"
                     /translation="MSGLGGLAVSGLDRGDSDLGSDLGDSDRGASVRVGSERVGSERG
                     VSDREGSERGDSERAGGSDLGGAERGVSERSTRAGGS"
BASE COUNT         1355 a         1428 c         1482 g         1423 t
ORIGIN      
        1 aattcaggac ctggttcaat ttaacgatcc tgtgtttgcg gctgcgaata atgatccaag
       61 ataccgggtt aaacctattc ccggccgaga ctcggataac tttgaacctc ggcttggatt
      121 caattggaat cctcgcacca gcagcaatgg ttttcttggt tggataactg gtggagacaa
      181 gctggttgtt cgcggtgggt atacgcgaac tcacgactat gcttacacca acatcgcttt
      241 gaacatttgg agctcgtttc catttgtcgc tgcatttaac ctgacaaatg ttccgaacgc
      301 ctttgctaac tacaaaaccc taccggttga tccggcaatc ttcacgcgca cgcaggtgac
      361 tgacgatttt cattcgccta cttacgactc gtttagcttc gagtttcagc gtgagataac
      421 gagagatttg gtcttccgtg tcgggtatgt cggctccaag ggcacaggac ttttcgaaag
      481 tatcgacggc aatcctgtac gcttccgcac ggccgttccg tctgctgcga acccggcaat
      541 cagaactgat ccgacaactg gtccaatcag attaagggcc aattcgggtt cgtccattta
      601 ccattcgatg caggcgagta tggagaaacg attgtccagt ggattcagcg ccggcgtcca
      661 tttcacctgg agttcattca tcgacagcat gtcggagatc tttaacaact ccaattctga
      721 aattgctttg gctcaggatc cctacaaccg tgcactcgat cgaggacgct catcatatga
      781 caggcccctg cgactggccg ggaactttgt atacgagctt ccaatctatc gggatcaaca
      841 aggctttgtt ggacgcttac tcggaggttg gcagctcaac tccaacttta acttccagag
      901 tggcgctccc ttctcgcctc tgaatccaag tgacctctcc ggaacacttg ggggattggc
      961 atcggcgatt ggtattgcga cccgaccaaa cgtcgccact aacatcaata tgtcaggaat
     1021 gagtgtagaa gatttatttg ctcttcgtgg aacggtcaat gcttccggca acgcactgtt
     1081 ccgtgcgctc caacctggtc agcgcatcgg cgacgctggt cggaatatcc ttcggtcaga
     1141 cggtctcaac aacgtcgatt ttggcttgct gaagaacacg aggattggcg agaatcagcg
     1201 gctgcaaatt cgcgccgact ttttcaatgc cactaatact cgtgactttg gtaccccaaa
     1261 ttcagtggtc acctcgactg gttttctgcg acagtgggac actaacggtg gtaatcgccg
     1321 aataatcgtc gggatccgtt acgtgtttta aggtttagac tctttcaatt ggagggttct
     1381 tcgctgaggt aaatgccttt gagaagaacc ctctcttctt tcgttatcgt ctgcttgtcg
     1441 agagctggac ccgaacctta ctgatcgtaa tcatcagcgt tgtagtatca taattgccca
     1501 cacttatctg aggagccagc gatgaaatat ttgatcctgc ctctcctttt tgtattaacg
     1561 gcgattagct ctctggcgca atcgtcaaag gaatctgcca gtgcacccgg cctggatata
     1621 attcgattta gctggagtaa agagcgtatt ggttggcaaa gagatccatt cagcgggccc
     1681 attgaaaact ttcaggaagt ccaggctcga acaagaaacg agaggcgagt acaagacgcc
     1741 aagcgtggag gtggtccaga ggtcgacaaa atcaagaccg aggcaaaagc agacgccgcc
     1801 aacattgcca cgcaacataa aaccgtaccc gctcgctacg tattcatgta caagttgacg
     1861 gtaaagaact cttcttctaa gaccatcaaa tctatcgatt gggattacgt gtttctggat
     1921 cgagcgactg gaagcgagtt gggtcgtcgt gaatttacca gtgtagaaaa agtctctccg
     1981 ggaaagtcga aagaattgac agtggtaatt tccaagccac caactcaaac cgtgagcttg
     2041 acggctctta actcaaatga gcgcgacgcc atgcttgaac ttgttagtct ggtcagaatt
     2101 gaatacacag acggcaccgc atggcagcgt ccgtgacggc agcaacacct tatggcgata
     2161 cgagcagcaa cagtcgatga ggccgaagag cttactgcga ttgcaatggc ggctaaacgc
     2221 cattggggct atcccgagaa ttggattaaa caatgggaag ccgatctgac cattacgtcg
     2281 gattttgttt cggacaatac cgtttttgtc gctgaggaag atggcgaagc aagaggcttt
     2341 tatgcgttat gcgtaaatga cggtaaagca gagttggagc acatgtgggt gaagccgcaa
     2401 tatatcgtca ctggagtcgg taaagaactc ttccttcacg cgatggacct ggcgacgacg
     2461 ttgaagttaa gtgaattgag catcaccgca gatccgaacg cggcctcttt ctatgagcat
     2521 atgggtgccg agcgagttgg agaggtaggc tcctcaatcg aaggccggaa attaccgcgg
     2581 ctgaagattg aaagggaata ggtcccattg cttaaaaccg ctcgatatat tcccgcgata
     2641 ccagaaaagc cttcaccatg tctgctgccc ggaaattccc attgaactga ttcagcttgg
     2701 tcaaccaaaa atcgtaacct gagaagttaa gatcaggcgc gtcatccgga tttcttctta
     2761 agtatccgaa gtactgcatc agcacgaacg ccttgttcaa ctcagcattt gtaaagtcca
     2821 catcgttagc taccttcaaa aggacggttg ctctcgtctc ctgaccaccg ctcaagccag
     2881 ccactagatt attgcgttca gttgttgata aagccccacc tgtttgtgta tttaacttgt
     2941 caacatactg tgcagccgtc atctgtaacg ggaaactcgt ctgaaactct gcacgcgaaa
     3001 caaaggcagc tacgaaggcc gtagtgttag cggccaactt agcctcccaa ccacttgcgc
     3061 cgactataac tccgtttccg atagtaccgc tgtcggcaag aaattcttga aacctcggta
     3121 gtcctcttgg tctttgtgct gaagccggaa acgaagcctt gtataagcga tggaccagga
     3181 agcccgtctg ctgaaactcg attgatagga agaaggcggc agaggtgtcg atgcgcttta
     3241 cttcacgaca atgaacgtca acaccgcaca agtcaatgtt attgacccaa aaaggaaaac
     3301 cagttgcatc gggctctcta ttgagaaaat caaggtagtg ttgtctgaca aaaaagcttg
     3361 tgtcatctaa tcgatttggg cctagaccaa tgcctgaaaa gccgaattgg ctaccgtcaa
     3421 cctgagcgaa tactttccca tcatctaaaa aaccatccag attcggatct ggaaatgcgt
     3481 ctacacgggc gattccgcca tttcctccgg taccgtcttc agaggaagta gcatcttcaa
     3541 taacgtaaac gtttcccaaa cgatcgatcg tgagcccgtg aaggaacaag ttgttatcaa
     3601 tcgaaaggcc ttcaaccaca acttcatcgt gatcggcgcg tccatccgag ttgtcatcgt
     3661 aaatcgccag cagaatagtg tcgttcccct cggaatgaga aaacaccacg ccgcgagaat
     3721 taactgcaat gtctaacggt gcgtcgtcag agaactgata cgagtagtac ggctgcgtat
     3781 ccagggtgtc aggtatgcga tcgccattag tgtcgcgtac gattctgagt tcgttggaag
     3841 caaagtcggc gatcagtaga tcgttgttcg gcattagggc gagggcgttt gcgttgaaca
     3901 gcttgttgtc acctacggta acaagagttc gtgaaaggga ttcgtcaaag ccgccggttg
     3961 ctgagtttcg aactagcagg attacgcctg gcgaaggttc gttattggga tcgttcggat
     4021 catctgagtt gaaatagcca cttgatgaaa cgatcacgaa ttcacgacca tttgacgaaa
     4081 gcccgctcac aactgccgct gctgaaggca agtcgaagtc gcgctccagg tcgaacacct
     4141 ccgaagtgtt tgccacgaag tctccgttac tatccgtcgc aactaccagt acgccaagaa
     4201 gagtatttcc atcaatggca gaatagacct catatcgtgc gcctgattgg cctacggcta
     4261 tgtcgaacga gccgccgtct gaatcaaatg tagttctgtc gtccgcgatt agatctttgg
     4321 ttctgtctac aaactgttcg ttcgtacccg ctgaagagtt gagagtaagt tgcgaagtat
     4381 gtaacacgcg agctagcgat gttcccgcga tgattggacc agccggcgcg ataatctgga
     4441 tggtggagga tcgcatcagc gcaggttggt ggcttgagct cgttgccctt tcagctagcc
     4501 tctgagatcg tttgcgcgtg gtcaggcttg cgggtctgta gccacccgtg cgtgtgttga
     4561 ttccaactcg ttgtgggaca cgaagtaccc ggcggatctc gagtttggcc ctggtcgttg
     4621 gcgtttgtgc gttaccaatg ccaacgagac aatttgtcca aagaattaat agggaaagaa
     4681 aagtcgaaat agagattagt aatcgattga attgccgcat cttcgtctcc atcggaaaac
     4741 aattgagggg ttgcggcctg ggcctggctg acgcatgtca acctacgggg tccaccggtg
     4801 gttcaggccg cttcctctct tcctttacga ttaaggctca cactccgaaa ctaacgatcg
     4861 ttgtcgggtt tgggaggcct tgccgtttca ggacttgatc gcggtgattc tgatctaggt
     4921 tccgatctag gagattctga ccgcggtgct tctgtacgcg taggttctga gcgcgttggt
     4981 tcagaacgag gcgtctcgga tcgagaaggc tcagaacgtg gtgactcgga acgcgccgga
     5041 ggttcggacc ttggtggcgc cgagcgaggc gtttctgagc gttcgactcg agcgggtggt
     5101 tcatagttat cgggtcgcga aggacgcgat ggagcttcgg agcgcggttc acttctaggt
     5161 gctggctcgc tgcgagttgg agcctcactt ctacttggag cctcgcttcg agttggagca
     5221 gaagattcgc ttcgacttgc cggcgcctca ctccgcggtg tcgagggttc gctacgcgtt
     5281 tcattgcgca ttggtgcgac tctgatctca tctcgcgatc gcttttcacc tgaatccgta
     5341 gcacgaggcc gattgtctgg cgacgttgaa ttgtccaccg acggtctcgt tggattgccc
     5401 acagttcttc gatccaccgg cgatctgtca ccactagagc gatcgcccga atcgcctcga
     5461 gttggtacag gccgcgtcaa tgctccggta ggtctgcttt caactgtccc gcccgtacta
     5521 gagttaccgt ccggttgaac cgtttgacgt ggctcacgat tgttgaagat tcgagatcgc
     5581 ctgagttcct catccatttg cgatccggtc gatcgcgtgg cagcgccggt tgctcgctct
     5641 actggctgga ttgcaggtgc tacgcgcaca ggtcggccca acacagcg
//
LOCUS       DASSFP010000454         9198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_285335, whole genome shotgun sequence.
ACCESSION   DASSFP010000454 DASSFP010000000
VERSION     DASSFP010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285335"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFH91_10230"
     CDS             complement(<1..327)
                     /locus_tag="VFH91_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863419.1"
                     /translation="MKRLLELAVLPLFVIGLIVLPVRAQSADGANSVEISKVISAFTA
                     KESEFRTALNNYSFKRDALLQSIGMGGQVIGEYHKVSLLTFDDSGNRYEKISFFPMPS
                     LAEITKE"
     gene            complement(861..1712)
                     /locus_tag="VFH91_10235"
     CDS             complement(861..1712)
                     /locus_tag="VFH91_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET6863420.1"
                     /translation="MTTPKERQKITLAHSPDSDDAFMFYGLATNKIDTGDLVFDHLLK
                     DIQTLNEEATRGTYDVTAISFHAYAYVADKYSLLPHGASIGDKYGPIVVAKEPASPED
                     IPRLKIAIPGKLTSAFLALRIFNDQFEYEVVPFDKIIDAVQDGTCDAGLLIHEGQLFF
                     ESKGLHKVLDLGEWWHERHGLPLPMGGNVIRKDLGNQRINEVSRLLRESINYSLTHRD
                     DALQYAMQFARDMDPHLADRFVAMWVNELTLDYTDRGRQAVRQLLEEGFEKGIIPNRV
                     SVEFAGD"
     gene            complement(1741..2856)
                     /gene="mqnE"
                     /locus_tag="VFH91_10240"
     CDS             complement(1741..2856)
                     /gene="mqnE"
                     /locus_tag="VFH91_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HET6863421.1"
                     /translation="MKFSHDDKLNEIASRVESGDRLGFEDGLALFATDDLPGLGKLAD
                     TARRKRHSRTTFFNVNRHLNPTNICYADCKFCGFFRTPRQPDAYTHNIDDSLKVAARA
                     VAEGATELHITGGLNTKLPFSYFTDLLSSLKREYPQLHLKAFTMVEFDHFAHFYKMSD
                     EEVIQSLIAAGLNSCPGGGAEIFREPTRSMICAHKCDADRWLELQGKVHAAGLKTNAT
                     MLYGHIESIEDRVDHLVKLREQQEKSHGFQCFIPLAFYPPDTQLAHLPGPSGIDSLKT
                     MAVSRLMLDNFDHIKAYWVMLGKRLAQVALHYGANDIDGTITDGGELTTSYSVEANNE
                     VRMSKPELIQLIEEAGFEAVERDTVYNRIERDRLSVV"
     gene            complement(2873..3739)
                     /locus_tag="VFH91_10245"
     CDS             complement(2873..3739)
                     /locus_tag="VFH91_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HET6863422.1"
                     /translation="MSTVLKNTRLTLEMIKIEHTLFALPFAFLGAVLAAGGLPTLRQL
                     FWITVAMVGARSAAMAFNRIADRNIDARNPRTASRAIPAGLLSTKFVWAFTIVASILF
                     LLAAAMLNRLTLVLAPIALGSVLLYSFTKRFTQFSHIVLGWCLAIAPTGAWIAVKGQF
                     GSPVPFLLSLVVLLWTAGFDVLYACQDYEFDRREGLRSIPARLGVASALWVSRGLHAG
                     AFLSLIALYLISGLGPFALAGVIFTGALLVYQHFLVRADDLSRLNAAFFTTNAFVSVI
                     LLITFGSALFLR"
     gene            4034..4798
                     /gene="larB"
                     /locus_tag="VFH91_10250"
     CDS             4034..4798
                     /gene="larB"
                     /locus_tag="VFH91_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET6863423.1"
                     /translation="MDLNELKNLLTAHKSGNLQTTEAIEAIKSLQFEDIGFARVDHAR
                     ASRQGFPEVVFGGGKTRDQIVGIVDRLAKRSPNILVTHTDEGTYGEIRNIATDAEWHS
                     SARLVRICRDKTDRGTGVIAVVTAGTSDIPVAEEAALTAETMGNKVDRVWDVGVAGIH
                     RVLSERTLLQRARVVVVAAGMEGALPSVVGGLVAVPVVAVPTSIGYGASFGGVAALLG
                     MLNSCSSNVTVVNIDNGFGAGFVASLINRRWEPADA"
     gene            4830..6293
                     /locus_tag="VFH91_10255"
     CDS             4830..6293
                     /locus_tag="VFH91_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863424.1"
                     /translation="MNPSVAPKTDVRSFLAFAILLALLLLFATSCISAKKTPNLEHIF
                     ASARTSTGKRPVIVIPGILGSELINTKTGEKVWPSAFRTTDEGLPITPDLSSNRDDLV
                     AGKIIETLKLSRVLPEVYVYRDLLEALRRYAGYRDGDWENPGADGGEDTFYVYPYDWR
                     QDNVTNARELVRRIERLKVKLNRPDLKFNIVAHSMGGLIARYAAMYGDADLPERETPI
                     QPNWAGARHISKIIMIGVPNEGSADAFATLLEGYSITEGLRRRVPLLNKLTAEDAVRT
                     PSVFQLLPHPAAVKFLDENLQPLTLDLFDPASWKKYGWSPIFSDEFRRKFASEEHRKV
                     SAHPSQNKLQILEAYLAETLYRARRFQQALDAGTDQSPVMLLAIGGDCEETLNAPVIL
                     RDQKKSRWLTLLRPIEYRTSAGIRISKRQVTEAMYAPGDGRVTRTSLLGQNLTSDHSR
                     RSIALQAVFGCDLHGQLQRNKSLQDNALTAIVGEMVK"
     gene            complement(6305..7804)
                     /locus_tag="VFH91_10260"
     CDS             complement(6305..7804)
                     /locus_tag="VFH91_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6863425.1"
                     /translation="MSQQSPRFGSQLLLYSEYMSPSISLTQIVLVVIIGIIGATPVRS
                     QDLLPDLVRRIKPSAVAIETFDARGEKLSRGSGFFIDVDRIATNRHVIEGAFRAEIHS
                     SSGNVYPVRGVLAVDAEGDIALLKIDAPTAPVRALTLDKTSPQEGESVVVIGNPLGLE
                     GSVTNGIVSAVRDIPTFGRIIQITAPISSGSSGSPVVNMQGQVIGIATLQITGGQSVN
                     FAIPSERISQLQIATLMPLAELVANTGRNKRAKAVQFFRDGLSFLSKDDCEKALTYFE
                     KATESDSTYADAWAQAGFCNEKLGRHAEALDASKKAVSIRPSAESYFNIGLASYYLKQ
                     YRESVEGYRQSIKLDPYNAADAYYALGLVYRDWGKPDDEIQAYKQAIRLRPDYTNAYE
                     RLGARYLKAKKFSEAVEIFRQLSQLKPGDAAAPNNMGEAYLELNRLSDAMEAFRQAIR
                     LKPDFGKAYFNLGKCLLAMGNRDAALEQYNILTNIDNDWAEKLNAIINP"
     gene            complement(7804..>9198)
                     /gene="proS"
                     /locus_tag="VFH91_10265"
     CDS             complement(7804..>9198)
                     /gene="proS"
                     /locus_tag="VFH91_10265"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET6863426.1"
                     /translation="IARNEINSYKQLPQVWYQIQIKFRDEARPKSGLMRLRTFIMKDA
                     YSFDVDRDGLDKSFIDQREAYKKIFTRCGIQFLIVEASSGAMGGTESNEFMARTPAGE
                     DLIVSCPKCGYAANLEKAISRLPVINDEPGPDKPEEFPTPGVRTIEDLIGFPGGAAAD
                     HQIKSLVFYATIDDKQQPVLVLLRGDHQLHETKLADNLKATAVRPARPEEIQDLLGAS
                     AGSLGGVGAKEKAAAAKLDLRIVADASLRERRNMTTGANKDDHHIRGVDINRDIKVDG
                     WFDLRTVEVGEDCPRCDDGLLEVFKGMEIGHIFKLGTKYSDSMGATVLTQEGKQVPIV
                     MGSYGIGIERIITAAIEQQHDDDGIIWPKSIAPFHVIVTITNMKQDDLRQAAERLYHD
                     LTKLGLDVLLDDRDERAGVKFKDADLIGVPYRITIGKKISDGLVELFDRRTKTTEDVG
                     LDKVAEHVKNLLTW"
BASE COUNT         2258 a         2272 c         2344 g         2324 t
ORIGIN      
        1 ttccttcgtt atttcagcca gggatggcat cgggaaaaag ctgatcttct catatcgatt
       61 ccccgaatca tcaaatgtga gaagggaaac cttatggtac tcacctatga cctgaccacc
      121 catcccaatg ctctggagga gcgcatctcg tttgaacgag taattattca gggcagtccg
      181 gaactcgctc tccttggcag tgaaggcact gattaccttc gatatttcca ctgaatttgc
      241 gccgtcagct gattgggcgc gaacaggcaa tacaatcagg ccgattacaa atagcggcag
      301 caccgccagt tccagaagtc tcttcataat tccaacttcc atcgtagaag ggttcaacaa
      361 ataggagggc aactgcagtt aaaagactgc cccgccgtca gatgcttttt caaaagccag
      421 ctgttgccag cctccgggtt ccgaccggta cgaatttctg tatttcctat tgcaaagttt
      481 cctgcatcta gtgtaaggtg ccaatattag aataagttag gccgaactgg cggcgctcaa
      541 aacgagttag attcggcccc ggttgaacta atcaacacgg tttgaaaaac ggtatctcaa
      601 ggtcctcatg tcggcagcag ccatattgtc ccaactccgt cgagctttac ggcgcgggag
      661 cgcagacgtg acgccgagcg gcggcgttgc ttgtacggat ctgggctaac caagctttgg
      721 gaggagcttg gttttaagtg gtctgaggtc gacgatgtgc gcggtgacat gtcactcctt
      781 ctgggcggag tggctgtaaa atcaccgcgc acaccccagc ctcaaaacga attgccctca
      841 agacctctcc tggaagaatt tcagtctccg gcaaattcca cggaaacacg gttcgggata
      901 attccttttt caaaaccctc ttccagtaat tgcctgacag cctgccggcc tcgatccgta
      961 taatctagtg ttaattcgtt gacccacatc gctacgaatc gatctgcaag gtgcggatcc
     1021 atatctcgtg cgaactgcat tgcatattgt agtgcgtcat ctcgatgggt caacgagtag
     1081 ttaatgctct cgcgtaataa gcgcgacacc tcattgattc gctgattccc caggtctttt
     1141 cggattacgt tacctcccat tggtaacggg aggccatgtc gctcgtgcca ccactcacca
     1201 agatccagga ctttgtggag acctttgctc tcaaagaata attgcccttc gtgaattaac
     1261 aagccggcat cacaagttcc gtcctggaca gcgtcgatga ttttgtcgaa cggaacaact
     1321 tcatattcga actgatcatt gaagatgcga agcgccagaa aggcacttgt taattttccg
     1381 gggatggcaa tcttaagcct gggtatatcc tcggggcttg cgggttcttt agcaacgaca
     1441 attggaccat atttgtcgcc aatcgaggct ccgtgcggta gtagcgagta tttatccgca
     1501 acatacgcgt acgcgtgaaa acttattgcc gttacatcat aggtaccgcg agttgcctcc
     1561 tcgttgagcg tctggatgtc tttaagcaag tgatcgaata cgagatctcc agtatcaatc
     1621 ttattggtag caagtccgta aaacataaat gcatcatccg aatctggaga atgcgcgagt
     1681 gttatctttt gacgttcttt aggagtggtc atttagaatc tttcgaaagg aatttgctgc
     1741 ttatacgaca cttagacgat cacgttcgat gcggttataa acagtatcgc gttcgacagc
     1801 ctcaaagccg gcttcttcaa taagctgaat cagttcgggt ttgctcatgc gaacttcatt
     1861 gtttgcttca acagagtaac tggtggtcag ttcacctccg tcagtaatcg ttccgtcgat
     1921 gtcattagcg ccataatgca aggctacttg agctagtcgt ttaccgagca taacccaata
     1981 ggccttgatg tgatcgaagt tgtccagcat cagccgcgaa accgccattg tctttagcga
     2041 atcaatgccc gatggtcctg gcagatgtgc gagttgggtg tctggtggat aaaatgcgag
     2101 tgggataaaa cattgaaatc catgcgattt ctcctgctgc tctcgcaact tcaccaggtg
     2161 atcgacacga tcctcgatcg attcgatatg tccatagagc atggtagcat tcgtctttaa
     2221 gccggcagca tgtacctttc cctgtaattc cagccagcgg tcggcatcgc acttgtgagc
     2281 gcaaatcatg gaacgcgtgg gttcacgaaa aatctcggcg ccacctcctg gacacgagtt
     2341 caggccggca gcaatcaagc tttgtataac ttcctcgtca ctcatcttat agaaatgcgc
     2401 aaaatggtcg aactcgacca ttgtaaatgc ctttaagtga agttgcgggt attcacgttt
     2461 gagtgatgag agcagatcgg tgaaatagct gaagggaagt tttgtgttca accctccggt
     2521 tatgtgtaat tcggtggcac cttcagcgac ggcccgcgcg gcgaccttca agctgtcgtc
     2581 aatgttatgg gtgtaagcgt caggttgcct tggagttctg aagaatccac agaacttaca
     2641 gtcggcgtaa catatgttcg tcgggttcag gtgacgattt acgttaaaga aagtggtgcg
     2701 gctgtggcgt ttgcgtcgag ctgtatcggc gagtttgccg agaccaggca aatcgtccgt
     2761 tgcgaagagt gctaaaccgt cctcgaagcc tagccggtcg ccactttcga cgcgactggc
     2821 gatttcgttc aatttgtcgt catgtgaaaa tttcatgttc accttaaact tcttatctta
     2881 ggaaaagggc actgccaaaa gtgataagca agattacgct aacaaaagcg ttcgttgtaa
     2941 agaaggcggc attcaaccgg cttaaatcat cggctcgaac caggaaatgt tgatagacaa
     3001 ggagtgcgcc agtaaatata acgccggcca acgcaaatgg accaagtccg ctgatcaggt
     3061 aaagcgcaat caaagagaga aaggctcctg catgcagacc tcgcgaaacc cacaatgcgc
     3121 tcgcaactcc tagccttgcg ggtatagagc gcaatccttc cctgcgatca aactcgtaat
     3181 cctggcaggc gtatagcacg tcgaatccgg cggtccacag caacaccaca agcgataaca
     3241 aaaacggaac agggctgccg aattgtccct taactgcaat ccacgcccca gttggcgcga
     3301 tcgccaggca ccaaccgagc acaatgtgcg aaaactgtgt aaatcgtttc gtaaaagaat
     3361 agagcagaac gcttccaagt gcgattgggg caagcacaag cgtcaaccta ttcaacattg
     3421 cggcggccag tagaaacaga attgaagcaa caattgtaaa agcccagacg aatttggttg
     3481 acaacaatcc cgcaggtatt gcgcgtgagg ctgttcgtgg attccgagcg tcgatgttgc
     3541 gatccgcaat tcggttgaaa gccatcgccg ctgaacgagc tccgaccatt gccaccgtga
     3601 tccaaaaaag ttgccggaga gttggcagtc ctccagccgc caggacagcg cccaaaaacg
     3661 cgaagggcag agcaaaaagt gtatgctcaa tctttatcat ttcgagcgtt aggcgagtgt
     3721 ttttaaggac tgtcgacacg gtgggtgaat cagaattggg tttattagtg attcgaaatc
     3781 ttacttggcg acacccatga gcgcaagcga gccaatcgaa ctgctcacca gagaatacga
     3841 gtttgaaaac gacgtagttt cgagggaata cgagtttgat gtgtagttta gagcttatga
     3901 atctctttac accgaaggtg aacgcgaatt tcagcccagg gttgaggttt ggcaaccctg
     3961 ggatggaagg tgtgctaggt ttatccaatc atttttgcct ctttgagcgg aatgctagaa
     4021 tccggtaact cctatggacc tgaacgaact caagaatctg ttgacggcgc acaaatccgg
     4081 taatctgcaa acaaccgaag ctattgaagc aataaagagt ctgcaattcg aagacatcgg
     4141 cttcgcacga gtggatcatg cccgggcgtc gcgtcagggt tttccggaag tagtttttgg
     4201 cggcgggaag acccgagacc agattgttgg catagttgat cgactcgcga aacgctcacc
     4261 caacatcctc gttactcata cggacgaagg tacctacggc gagattcgaa atattgccac
     4321 cgatgctgaa tggcattcct cggccagact agtgcgaatt tgtcgggaca agactgaccg
     4381 tggaaccgga gtaattgctg tagtaacagc tggaacgagc gacataccgg ttgctgaaga
     4441 agcagcattg acagccgaaa caatgggtaa caaggtggat cgtgtttggg atgttggagt
     4501 tgccggtatt caccgggtgc tgtccgaacg aacgctttta cagcgcgctc gagtagtagt
     4561 tgtagccgca ggtatggaag gagctttgcc ctcagtggtc ggtgggttag tggctgtccc
     4621 ggttgtggct gtgccaacca gtattggtta tggtgcatcc tttggaggag ttgccgcatt
     4681 gcttggaatg ctcaacagct gctcttcaaa tgtgactgtg gtaaacattg ataacggttt
     4741 tggcgcaggc ttcgtagcaa gtctgataaa tcgacgctgg gagccagctg acgcctgaaa
     4801 acaaaaacac acgttaagtt ggtcccattt tgaatcccag cgttgctccc aaaacagatg
     4861 tccgttcttt cctagccttt gcaattctcc tggcgctgct tctgttattc gcgaccagtt
     4921 gtatttcagc caagaaaaca cccaatctcg aacatatttt tgcttcagcg aggacatcaa
     4981 ccgggaagcg gccagtcatt gttatcccgg ggattttagg atctgaactg attaatacta
     5041 agaccggtga aaaagtatgg ccttccgcct ttagaaccac agacgaagga ctaccaatca
     5101 cacctgacct ttcgtcgaat agagatgatc tggttgccgg gaaaatcatc gaaactttga
     5161 agttgtcacg tgtgcttcca gaagtttatg tctatcgaga tctactcgag gcgctgcggc
     5221 gttacgccgg gtacagggat ggcgattggg agaatcccgg tgctgatgga ggagaggata
     5281 cgttttacgt ctacccatat gattggcgtc aggacaacgt caccaacgcg cgagaacttg
     5341 tgagacggat cgaacgtctc aaggtcaaat tgaatcgccc tgatctaaag ttcaatatcg
     5401 tcgcgcattc gatgggtgga ctaatcgcca gatatgcggc gatgtatggt gacgcagatt
     5461 tgcctgagcg tgaaacgccg attcaaccaa attgggctgg cgccaggcac atcagcaaaa
     5521 tcataatgat tggtgtacca aacgaaggct ctgcagatgc ctttgcaacg ttgcttgaag
     5581 ggtattcgat tactgaagga ttgcgccggc gcgttccgtt gcttaacaag ttgaccgcag
     5641 aagacgcggt ccggacacca tcggttttcc aactcttacc tcatcctgcg gcggtgaaat
     5701 ttctggatga gaatttacaa ccactcacct tggacctttt cgatccagcc tcgtggaaga
     5761 agtacggctg gtcaccaatt ttctccgatg agtttcgacg caaattcgca tcggaagagc
     5821 accgcaaagt tagcgcccat ccgagtcaaa acaaactcca gattcttgag gcttatctcg
     5881 cggagacgct ttatcgtgcg cggcgctttc aacaggcact tgatgcaggc acggatcagt
     5941 cgccagtgat gttgctggcc atcggaggag actgcgaaga aacgttaaac gcgccagtga
     6001 tcttaagaga tcaaaagaag agtcgctggt taacgctgtt gcgccctatt gagtatcgga
     6061 cgtcggcggg gatcaggatt tccaagcgac aggtaaccga ggcgatgtat gcgcctgggg
     6121 atgggcgcgt tactcgcaca tcactgcttg gtcagaatct gacatccgac catagccggc
     6181 ggagtatcgc tttgcaggct gtattcggct gcgatcttca cggacagttg cagcggaata
     6241 agtcgctaca agataacgcg ttaaccgcaa tcgttggtga aatggttaag tagttactgt
     6301 gggatcaggg gttgatgatt gcgttgagct tttcagccca atcattatca atgttggtga
     6361 gtatgttgta ctgttcaagc gcggcatctc gatttcccat cgccagtaga cacttaccta
     6421 gattgaaata agctttgcca aagtctggtt tcagcctgat cgcttgtcga aacgcctcca
     6481 tcgcgtcgct cagccggtta agctcaagat aagcctcgcc catgttattc ggtgcagcgg
     6541 cgtcgccggg cttgagctga gagagttgtc gaaatatctc gacggcttcc gagaatttct
     6601 tagctttcag atatcgggct cccagtcgtt cgtaagcgtt tgtgtaatcg ggtcgaagac
     6661 ggatcgcctg tttatatgcc tgaatctcat cgtccggctt accccaatca cgatagacca
     6721 gaccaagcgc gtaataagca tcggccgcgt tgtagggatc cagttttatg gactggcggt
     6781 agccttcaac cgattcgcga tactgtttca aatagtaact ggcgaggccg atgttgaagt
     6841 acgactcagc ggagggtcga atcgaaacag cctttttgga cgcgtccagg gcttcagcat
     6901 gcctaccaag tttctcattg caaaatcccg cttgagccca agcgtccgcg taagtgctat
     6961 cagattcggt agccttctca aaatatgtga gagctttctc gcaatcgtcc ttcgaaagaa
     7021 agctaagtcc atcgcggaag aattgaaccg ctttggctct cttgtttctc ccagtgttgg
     7081 ctaccaactc tgccaaaggc attagcgtag cgatttgtaa ttgggagatg cgttcggagg
     7141 gaatcgcaaa gttgacactc tgtccgcctg taatttgcag cgtcgcaatg ccaataacct
     7201 gcccttgcat atttactacc gggctaccgc tggatccaga ggagataggc gccgttattt
     7261 gaattattct tccgaatgta ggtatgtcgc ggacggctga aacgatcccg ttagtcacac
     7321 tgccttcgag gcccagtgga ttcccaatca ctactacact ctcaccttct tgtggtgagg
     7381 ttttatcaag cgtcaatgca cggactggcg ccgtgggtgc gtcgattttc agcaacgcga
     7441 tgtcgccttc cgcatcgacg gcaagtactc cccttactgg atagacattt ccggaagatg
     7501 agtgaatctc ggctcgaaaa gcgccttcaa tcacgtggcg attggtggct atgcgatcga
     7561 cgtcgataaa aaagccactg cctctcgaga gtttttcgcc acgcgcgtcg aatgtttcga
     7621 tcgcgaccgc agaaggcttt atgcggcgca caaggtccgg aagcagatcc tgcgacctca
     7681 ccggagtcgc gccgatgatt ccgataatga cgactagaac aatttgcgtc agggaaatgc
     7741 tcggactcat gtactcagag tataacagca gttgggatcc aaagcgggga gactgctgcg
     7801 acattaccag gtcagcaagt tcttcacatg ttccgccacc ttatcaaggc cgacatcctc
     7861 ggttgttttt gtgcggcgat cgaatagttc aacgaggcca tcgctgatct ttttaccaat
     7921 cgtaatgcga tagggtacgc caattagatc agcatctttg aattttactc ccgctcgttc
     7981 gtcacgatca tcgagaagca catccagtcc gagctttgta agatcatgat aaagtcgctc
     8041 ggcggcctga cgcagatcgt cctgcttcat atttgtgatg gttactatta cgtggaacgg
     8101 ggcgatagat ttgggccaga tgatgccatc gtcatcgtgt tgttgttcga tggcagcagt
     8161 gattatacgt tcgatgccaa taccataact tcccatcacg atcggaacct gcttaccttc
     8221 ctgcgtgagc actgtagcgc ccatcgaatc tgagtatttt gttccgagct taaagatgtg
     8281 accgatctcc attcctttga agacttctaa taatccatca tcgcaccgag gacagtcttc
     8341 gccgacttca actgttcgga ggtcgaacca tccgtcaacc ttgatgtccc gattaatatc
     8401 aactcctctt atgtgatggt catccttgtt ggcgccagta gtcatattgc gacgctcgcg
     8461 caatgaagcg tcagccacta tgcgcagatc gagtttggcg gccgcggctt tttcctttgc
     8521 gcctacgcca cccaaactac cagcgctagc gccgagtaga tcttggatct cttccggtcg
     8581 tgcgggtctg accgcggttg ccttcagatt gtcagcgagc ttggtttcgt gaagctgatg
     8641 atcgccacgc agtagcacaa ggactggttg ttgtttatcg tcaatagttg cgtagaacac
     8701 cagagacttt atctggtgat ctgctgctgc tcctccggga aagccgatca aatcttcaat
     8761 cgtgcgtaca cccggcgtcg gaaactcttc tggcttatca ggcccaggtt cgtcgttgat
     8821 gactggcagt ctcgaaattg ccttttcaag gtttgctgcg tatccgcact ttggacaact
     8881 gactatcaga tcctcacctg ccggagttct cgccatgaac tcgttcgatt cggtgcctcc
     8941 cattgcccca gatgatgctt cgacaataag aaactgaatg ccacaacgcg taaaaatctt
     9001 tttgtaggct tcgcgctgat cgataaacga cttatcaagg ccgtcgcggt caacatcaaa
     9061 ggaataagcg tccttcatga tgaaggttcg cagacgcatc aagccggatt taggccgagc
     9121 ttcgtctcga aacttgatct gaatttggta ccaaacctgt ggcagttgct tgtatgaatt
     9181 gatttcgttc cgagcgat
//
LOCUS       DASSFP010000455         7140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1326444, whole genome shotgun sequence.
ACCESSION   DASSFP010000455 DASSFP010000000
VERSION     DASSFP010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326444"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFH91_10270"
     CDS             complement(<1..366)
                     /locus_tag="VFH91_10270"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET6863427.1"
                     /translation="MKIYPYCLVSSRVLLPPSIHGLDGRPILQQDIGGLTVILSESDA
                     EKVALTRENVLRHDAVVRSVLEHETPLPFRFGHLVTLEQLTSYIESKSESLQARLQQL
                     RGRVEMSVKIIWNRESKLTN"
     gene            complement(373..636)
                     /locus_tag="VFH91_10275"
     CDS             complement(373..636)
                     /locus_tag="VFH91_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HET6863428.1"
                     /translation="MSSPFDQITIDGESNFDDADVSLLETLDHVLNRGLVIAGEITIA
                     VADIDLIFIGLNVLVSSVEKAQEVLRERGRDPLDSESVADKVK"
     gene            complement(663..1499)
                     /locus_tag="VFH91_10280"
     CDS             complement(663..1499)
                     /locus_tag="VFH91_10280"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET6863429.1"
                     /translation="MGSNENNKRESGGSVLYVYCIAETGPAKEILAESSPPAIEDDAA
                     VRLVESGNLAAIVSSVSIASYGEERLSENLTDASWTALRAMRHEHVVEHFASRTSVVP
                     LRFGTIYLETGRIEKMLAENSPRLKEIVERLRDREEWGVNVYSDRTLLMSAITSLSPR
                     LKDLAERAGTASPGESYLLQKKIETLKVDEARAETNRAIGEIEELLRSESVDARRLRV
                     LKVEATEHGELKAKFAFLIQRSHFQRFQSAAEKIARELKSSGISLELTGPWPAYNFAG
                     EQ"
     gene            complement(1477..3582)
                     /locus_tag="VFH91_10285"
     CDS             complement(1477..3582)
                     /locus_tag="VFH91_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET6863430.1"
                     /translation="MTAPRRTSTRSRVRTDLATSRESPNNISNSRYLLFGGKGGVGKT
                     TAAAATAFHLLATATPDQSILLFSTDPAHSLADSLEIAVGNKLTEVARKNGARIVAFE
                     MDAGLALENFRNQHGPVLAEIAERGTFLDESDISELLNLSLPGLDEVMALFELSELDR
                     SGGFEHVIVDTAPTGHTVRLMQLPEIFDRMVNALDRMSDKHRYIVAQFARRRTLNDPV
                     DLFLADLKERIERVRLLLHDHESTRFALVSIPEALSVFEAQRYYRELQLLGVNITDLI
                     VNRVELPHENCRFCLARARNQKPWLAELEKSFSSLNSHLVPLQKQDVRGAEDLIRIGD
                     LIWKSNPKLVPLPGRTPGRTPSHKADSIVVEEGVGFSLNPRKVVIFGGKGGVGKTTSA
                     AAFALALAKSDKSKKILIFSTDPAHSLSDSFAGKVGPLKKRIAGFGNLSGMEIDPSKW
                     LDDLKERYQSWIDNLFSSLSTNSGLEIQFDREAMRELVQLTPPGIDEIAALGSISDLI
                     DEGVYDTIVIDSAPSGHLIRFLELPQVTLSWVRTFIKLLLKYQQIVRGTQIAEELVSL
                     SRSIKAIQALMTSADQCEFIAVAIPELMSLEETIDLTKSLRKLNIPMTRLLVNNLVPE
                     TAAHDCSFCRSRLELQNSVVQQFVKHFGEETSIFLAPQQPNEVRGLESLLKHFQSWSR
                     FESNKRSAKKIKRGLKRKQ"
     gene            complement(3586..3909)
                     /locus_tag="VFH91_10290"
     CDS             complement(3586..3909)
                     /locus_tag="VFH91_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HET6863431.1"
                     /translation="MFLVDDILLAPVSGFKFILGQIQKMADRELNDDTVIKEQLLELQ
                     MRLELDEISDAEYQEREAELFARLRALKTRQLELLHQAHTAESSSFVIEVGGDSQNDF
                     WEPGE"
     gene            complement(3915..4733)
                     /locus_tag="VFH91_10295"
     CDS             complement(3915..4733)
                     /locus_tag="VFH91_10295"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET6863432.1"
                     /translation="MSREAEQTNTDPVTEGKYVYCIIETSEPRSFGQMGIGGRGDEVY
                     LIRHRGLAAVVSNTPLVVYDPTRENVFAHEQVNETVMREFTVLPMAFGALFRTEDDII
                     ELIRGTYDALRDVLTKMEGKVEFGLKVNWDRDKVTLELEEKDDEIRHLKEQITSRTTG
                     STYFARMQLGRLIETALTDQADAYIREVYSLLRDTAVASRANKPIGDRMIMNAAFLIE
                     RDRENEFDQKVKEIASKYEGKLSFKYTGPWPPYNFVHIRLKLERGEGTSEAVAS"
     gene            5133..5279
                     /locus_tag="VFH91_10300"
     CDS             5133..5279
                     /locus_tag="VFH91_10300"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET6863433.1"
                     /translation="MLWTILVILLVLWLIGMLSHVGGGLIHLLLVVAAIVLVINLVTG
                     RRAV"
     gene            complement(5412..7013)
                     /locus_tag="VFH91_10305"
     CDS             complement(5412..7013)
                     /locus_tag="VFH91_10305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6863434.1"
                     /translation="MKILLYNPDNGVTRNFMPHLWMFLLQALTPPGHEVLLVDGNAKA
                     MTDQELVEFINREKIGLVGIGAMTRMIAKAYRVADTIRQAGIPVVMGGPHVTEVPDEA
                     LGRNGSGPRHADAVALGEADETWPLIVEDAANGTLKEIYKPATDKKGLDLKPTLQPYP
                     EIPWDTLDLAQFSLIPRILNPIMSKMGSGWGTFHVVPVETGRGCPYGCEFCTVTGFFG
                     DAIRFRSNKSVVEELLRLKARARRQLGQIAVFFIDDNLAINMKRIKSLLREIIEAKAQ
                     VPWVAQISANLLRDEELVDLIAESGGKWIFIGMESIDPANMADVNKNFSKPGEYRGVL
                     ERLAQRNIYGITSFIFGMDNDTVGVADRTLNEIGSWPPGLPVFGQLTPFPATPLYERL
                     DKAGRLARPKHWLDFAPFVMAHDPLHMSIEEARAETQHAWAASYSPERNAAAVKSLRN
                     APLQFRISHFVARLFFRGIYFPQLGWRSWVKVMHQNRMTILRLVQDSIGEILKSQPST
                     PIDAAGVKIPTMQTQVGEKPSQEPA"
BASE COUNT         1738 a         1857 c         1653 g         1892 t
ORIGIN      
        1 attcgtcaat ttactttcgc gattccagat aattttgacg ctcatctcta ctcgaccccg
       61 cagttgctga agtcgcgctt gcagagactc tgacttgctc tcgatataac tggtgagctg
      121 ttccagcgtg acgagatgtc caaacctgaa gggtagcggc gtttcatgct cgagcacact
      181 ccgaactacc gcgtcgtgcc gtaacacgtt ctcgcgcgtc aacgccactt tctcagcgtc
      241 ggactcactt aaaattacgg tcaaccctcc aatgtcctgt tgaaggatgg ggcgtccatc
      301 taatccatga atcgacggag gcaaaagtac gcgactgctc acaaggcaat agggataaat
      361 cttcatgtca aactacttca ccttgtcagc gacactctct gagtcgagag ggtctcgtcc
      421 acgctcacgc aagacctcct gggccttttc aaccgaactg acgaggacat tcaaaccaat
      481 aaagatcaaa tcgatgtcag ccaccgcaat tgtgatctca ccagctatga ccaatcctct
      541 gttcaaaaca tgatcgagtg tttccaacag agagacgtct gcatcatcaa aattggactc
      601 accgtcaatc gttatttggt caaaaggaga actcatgata attcgctctc gccgattcct
      661 tattactgtt cacccgcgaa attataggcg ggccatggtc cagtaagttc gaggctaata
      721 ccactcgatt ttagttctct cgcaattttc tctgcagccg actgaaatcg ttgaaagtgc
      781 gagcgttgaa tcaaaaaggc gaactttgct ttgagttctc catgctctgt ggcctcaacc
      841 ttcagcaccc gcaggcgtcg ggcatcgaca ctctcagacc tgagcagttc ctcaatttcg
      901 ccaatggcac gattagtctc agctcgcgct tcatctacct tcaacgtctc aatcttcttc
      961 tgaagtagat aagactcacc gggggatgcc gttccagccc gctctgctag gtccttcagt
     1021 cgaggagaca atgaagttat agctgacatc aacaaggttc gatcggaata aacattcacg
     1081 ccccactctt cgcggtctcg taagcgttca actatttcct taaggcgtgg gctattttct
     1141 gccagcatct tctcgattct gccggtctcg agatatatag tgccgaaacg cagaggaacg
     1201 acactcgttc ggctggcaaa gtgttctacg acatgttcat gtctcatggc tcgcaacgcg
     1261 gtccatgaag catcagtcaa attttccgac agcctttcct ccccatatga ggcaatggag
     1321 actgaactga caatcgctgc gaggttacct gactctacta gcctaactgc ggcatcatcc
     1381 tctattgccg gcggcgacga ctcggccagt atttcttttg ccggcccagt ttctgctatg
     1441 cagtaaacat acagaactga cccgccactc tctcttttat tgttttcgtt tgagcccacg
     1501 tttgattttc tttgctgaac gtttattaga ctcgaacctg gaccacgatt gaaaatgctt
     1561 tagtaaactt tctaaacccc taacttcatt tggctgctgc ggcgccagaa agattgaggt
     1621 ctcttcaccg aagtgcttga cgaattgctg cacaactgaa ttttggagtt ctaaacgact
     1681 cctacaaaaa ctgcaatcat gggctgccgt ttccggcacc agattgttaa ccagtaacct
     1741 cgtcattgga atattcagtt tccgaagcga tttcgtcaaa tcgatcgtct cttccaggct
     1801 catcaactct ggaatagcca cggcaatgaa ctcacattga tctgccgatg tcatcaaagc
     1861 ctgaattgct ttgatgctac gcgaaagtga caccagttct tcagcaatct gggttccgcg
     1921 cacaatctgc tgatacttga gcaaaagctt aataaacgtc ctcacccagg agagcgttac
     1981 ttgtggcagt tcgagaaagc gaatcaggtg accacttgga gccgagtcga tgacgattgt
     2041 atcgtaaacg ccttcatcga ttagatcact gatcgaccct agagctgcaa tttcatcgat
     2101 gcccggtggc gttaactgga ccagttcgcg cattgcctct cgatcgaact ggatctccaa
     2161 tcctgagttt gtcgacaatg agctgaacaa gttatcgatc caggactgat aacgctcctt
     2221 aagatcgtcg agccattttg atgggtcgat ctccattccc gaaagattgc caaagccggc
     2281 aatgcgtttc ttaagcggtc ctacctttcc tgcgaagctg tcagaaagcg aatgagccgg
     2341 atccgtagag aaaatcaaaa tctttttgct cttgtcagat ttagcaagtg cgagagcaaa
     2401 tgcagcagca gaagttgttt ttccaactcc tcccttgcca ccaaagatga ctactttcct
     2461 ggggttcagt gaaaacccca cgccctcttc tacgactatg gaatcagctt tatgcgaagg
     2521 cgttctccca ggcgttcgcc caggcaacgg aacaagtttg ggatttgatt tccagatcaa
     2581 atcaccaata cgaatgaggt cttccgcgcc acggacgtct tgtttttgca gaggaactaa
     2641 atgggaattg agtgaactaa agcttttctc caactcagcc agccatggtt tttgatttct
     2701 cgctcttgcc agacaaaacc tgcagttttc atgaggcaat tccactcgat taacaattag
     2761 atcagtaata ttgacgccga gtaattgtag ttctcgatag tatctttgag cttcgaacac
     2821 gctaagggcc tctggaatgg acaccagcgc gaatcttgtt gactcgtgat cgtgaagtaa
     2881 tagtcgaacc cgctctattc gctctttgag atcagcgaga aagagatcca ccggatcatt
     2941 gagcgttctc cttctggcga attgcgccac aatataccgg tgtttatcac tcatccgatc
     3001 aagagcatta accattcgat caaagatctc tggaagctgc atcaaccgaa cagtgtgacc
     3061 ggttggcgcg gtatcgacga ttacgtgttc aaaacctcca ctgcgatcga gctcgcttag
     3121 ttcgaacagc gccatcacct catctaatcc gggcagtgaa aggtttagta gttcacttat
     3181 gtcagactca tccaggaaag tgccacgctc agcgatttca gccagcactg gtccatgttg
     3241 attgcgaaag ttctcgagag ccaggccggc gtccatttca aatgcaacga tcctcgcccc
     3301 attcttcctg gctacctccg ttaatttatt tccgacggcg atttcaagac tgtcggcaag
     3361 cgaatgagct ggatcagttg aaaacagtag tattgattga tcaggcgtgg cagtcgctaa
     3421 taaatggaat gcggtcgctg cggcagcggt cgtcttgccc acaccacctt taccgccaaa
     3481 caagaggtag cgactgtttg atatattgtt tggggattcg cgtgaggttg cgagatcggt
     3541 tctgactctt gaacgcgtcg acgtcctgcg cggggcagtc acgccttatt ctcctggttc
     3601 ccagaagtcg ttctgagagt cgccaccgac ctcaatcaca aagcttgaag attcggctgt
     3661 gtgagcctga tgaagcaatt ccagttgtct ggttttgaga gctcgcagac gcgcaaagag
     3721 ttctgcttct cgttcctgat attcagcgtc ggagatctcg tctagctcaa gtcgcatctg
     3781 tagctcgagt agttgttcct tgatcaccgt gtcgtcgttc aactcccggt cagccatctt
     3841 ctgaatctga cccagaatga acttgaagcc actaaccgga gcaagaagaa tgtcgtctac
     3901 gagaaacatc gtttctaact cgccactgct tcactggtgc cttcgccccg ctcaagctta
     3961 aggcgaatgt gtacgaagtt ataagggggc cagggtccgg tgtatttaaa cgacaactta
     4021 ccttcgtact ttgaagcaat ctcttttact ttttgatcga attcgttttc gcgatctcgt
     4081 tcaatcaaaa acgctgcatt catgatcatc cgatcgccaa tcggtttgtt ggctcgcgac
     4141 gcgacggccg tgtcgcgtag caatgagtag acttcgcgta tgtacgcatc ggcctgatcg
     4201 gttaaagctg tctcaatcaa tcggcccaac tgcatgcgcg cgaaataggt tgaaccggta
     4261 gtacgagacg tgatctgctc tttaaggtgt cgtatttcat cgtccttttc ttcgagttcg
     4321 agcgtcacct tgtcgcgatc ccagttcacc ttcagaccga actcgacctt tccttccatt
     4381 tttgtgagca cgtctcgaag tgcgtcgtag gtcccgcgaa taagttcgat aatgtcatct
     4441 tcggtacgga acaatgcgcc aaacgccatc ggtaggaccg tgaactccct catgacggtc
     4501 tcgttcacct gttcgtgggc aaaaacgttc tcccgcgtcg ggtcataaac aaccagcgga
     4561 gtattcgata caacagccgc gagtccgcga tgtcgaatca ggtatacctc gtcgccgcgg
     4621 ccaccgatac ccatttgacc aaacgagcgc ggttcggagg tctcgataat gcagtaaacg
     4681 tactttccct cggtaacagg gtcagtgttt gtttgttcag cttcgcggct catgttgttt
     4741 tccgttagcg gatgagttag gtagcgatta ccaccgtgag attggattct atcaagcgag
     4801 cggaatcata atcagtttgt aggagactgt acagcggcat ttttcggaaa ctcataaatt
     4861 ttcggaaact cataaattag agtctcccaa tggatttcaa tgggattacc gacattttga
     4921 gtgagcaaac acgatctctg accggttctg atgcatccgt ttgaatgcct actatcagat
     4981 tcagttcact ttcacttgta aacctatgta tcagcgcgga aatggtctag aattagcgcc
     5041 acaagacctt cccccaattg ctggcatgtc cgttgctctt gacgagagta ctacaggttc
     5101 atacacgcgt aagctaaaag gagattcgtt ctatgttatg gacaattctg gttatcttgt
     5161 tggttctctg gctgattgga atgctttccc acgtaggcgg aggattaatt catctgctat
     5221 tagtcgtcgc cgcgattgtc ctggtgatca atttggtgac aggtcgaaga gcggtttaga
     5281 aaggcgccgc tctgtgcggt gaggccacat caagttatgg tcggccgtct ggattgtgaa
     5341 gggatctgag gctaacagta gtcggctttc tactatctgc tgtccgccaa aggaactgaa
     5401 aacaactaga cctatgcagg ctcttgggat ggtttctctc cgacttgggt ctgcatagtc
     5461 ggaattttga cacccgcggc atcgataggc gtcgaaggct gcgatttcaa aatttctcca
     5521 atcgaatctt gcacgagccg caaaatcgtc atgcggtttt gatgcattac cttgacccac
     5581 gagcgccaac ccagttgagg aaagtaaata cctcggaaaa acagccgcgc tacaaaatga
     5641 cttattcgaa actgcagagg agcattcctt agcgacttca ctgcagcagc gttgcgttcg
     5701 gggctatacg aggctgccca cgcatgttga gtttctgcgc gcgcttcctc aatactcatg
     5761 tgcaacgggt cgtgggccat tacaaagggt gcaaaatcca accagtgttt gggtcgagct
     5821 aaccttcctg ccttatctag cctttcatag agaggggtcg ctggaaacgg agtaagttga
     5881 ccgaacaccg gcagtccggg cggccagcta cctatttcat ttaaagttcg gtccgcaact
     5941 ccaaccgtgt cgttgtccat tccgaaaatg aacgaggtaa tgccgtaaat gtttctttgg
     6001 gccaaacgct caagaacccc tcgatattct cccggcttac tgaaattctt attcacatcg
     6061 gccatgtttg ccgggtcaat cgactccatt ccgataaaaa tccatttccc gcctgactcg
     6121 gctattaaat cgacgagttc ttcatcgcgt aggagattcg cgctaatttg cgcgacccat
     6181 ggtacttgag cttttgcctc aataatctcc cgcaacaacg acttgatccg cttcatgttg
     6241 atagccaggt tatcgtcgat gaagaagacg gcgatctgtc caagctgtcg tcgcgctcgc
     6301 gccttcagac gcaagagttc ttcaaccaca cttttgttcg agcggaagcg aatagcatct
     6361 ccgaagaatc ccgtgaccgt gcaaaactca caaccataag gacagccacg tcccgtttcg
     6421 accggtacga catgaaaagt gccccaaccg cttcccatct tcgacatgat cggattcagt
     6481 attcggggaa taaggctaaa ttgagctaga tccagggtgt cccagggtat ttccgggtag
     6541 ggctgcagag ttggtttgag atcgagtcct tttttatccg tagccggttt gtagatctct
     6601 ttcaacgttc catttgcagc atcctctact attaaaggcc aggtttcatc agcttcaccc
     6661 agtgcaactg catcagcgtg gcgaggaccg ctgccattac gcccgagcgc ctcatcgggt
     6721 acttcggtca cgtgaggacc acccatcacc accgggattc ctgcctgacg gatcgtatcg
     6781 gccactcgat aagcctttgc tatcattctt gtcattgcac caattccgac caatccaatt
     6841 ttttctcgat tgatgaactc aacgagctct tgatcagtca tcgccttggc gtttccatca
     6901 acgagcaaaa cctcatgacc aggaggagtc agcgcctgca agaggaacat ccataggtga
     6961 ggcatgaagt tgcgcgtaac tccgttgtcc ggattataaa gaaggatttt catttaaaat
     7021 tctcacgatc aatacagctg acgactgggc atcaggccca aaacttatag gtagggaggg
     7081 agaatgaaaa tggcaggaaa aaattttgat taagttatgc cgctcttcat tatggccagc
//
LOCUS       DASSFP010000456         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_137239, whole genome shotgun sequence.
ACCESSION   DASSFP010000456 DASSFP010000000
VERSION     DASSFP010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137239"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /gene="asnS"
                     /locus_tag="VFH91_10310"
     CDS             complement(<1..1236)
                     /gene="asnS"
                     /locus_tag="VFH91_10310"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET6863435.1"
                     /translation="MTTPTSASTSEKQTYINQLSAHEGSEVLLKGWLYNMRSSGKILF
                     PQLRDGTGVVQCVVLKSNVSPEEWESLKAAGQESAVIIRGTVRADSRAPGGYEIDVLH
                     SQVLQQVHDYPITPKEHGTEFLMDHRHLWLRSRRQHAVLKVRHTVVQSVRNFLDNDGF
                     TLCDSPIFTPAACEGTTTLFEVDYFEGEKAYLTQSGQLYNEATAAAFGKAYCFGPTFR
                     AEKSKTRRHLTEFWMVEPEMAYATLEDVFSLAERMLSYIAARVLEDRKEELKVLERDT
                     TKLESIIAPFPRVHYDDAVKMLQEGFEKGLLESKFEWGGDFGAPDETYISSQFEKPVM
                     VHHYPAAIKAFYMARDPEREELALGVDVLAPEGYGEVIGGGERATSLEFLREQIALHE
                     LPEEAFQWYLDLRRYGTVPH"
     gene            complement(1257..2297)
                     /locus_tag="VFH91_10315"
     CDS             complement(1257..2297)
                     /locus_tag="VFH91_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6863436.1"
                     /translation="MSSATQVIPNAFESPASERLARFITGHGGSNDEIVPLTPDASTR
                     KYFRVPWQKGSAVAAVYQEPFDPEFHPYLDVTRLFLEANLPVPQVYEVNGGEGIIIQE
                     DLGNRQLFQIFDTETDETCDDYKEMAIGLIAKIQSATQRAVETHSIASRLAFDEAKLS
                     WELDFFVEHYFGSLRHDTLRHGEIAELKAELNDIAAELSAVPRVLCHRDYHAANLMVD
                     GKTRLRIIDHQDARMGPATYDLVSFLLDRQPCPPSLAELRAHRLFFLDQRRRQGLAVL
                     DPDEFAQEFRLMTIQRGLKAIGTFSYQTAVCGRGLFYEQFIHPTLRIVLQAADWLERF
                     PTLRRMLRERVY"
BASE COUNT          617 a          679 c          650 g          606 t
ORIGIN      
        1 gtgcggcacc gtgccatagc gtctcagatc caggtaccac tggaacgctt cttcgggtag
       61 ttcatgcagc gcaatctgtt cacgcaaaaa ctcgagtgac gttgcacgct caccgcctcc
      121 gattacttca ccgtaacctt caggtgccag aacatcgact ccgagtgcga gttcttcgcg
      181 ttcaggatct cgagccatgt agaaagcttt aatagccgcc ggataatgat ggaccattac
      241 cggcttctcg aactgactgg agatgtacgt ctcgtcaggt gctccaaagt cgccgcccca
      301 ctcaaatttg gattcgagca accctttttc aaatccttcc tggagcatct tcacagcatc
      361 atcatagtga acgcgaggaa agggggcgat tattgattcc aacttagttg tgtcgcgctc
      421 gagaactttc aactcctctt ttcggtcctc cagaacgcga gcagcaatgt aggacagcat
      481 ccgctcggcc aggctaaaga catcttcgag cgttgcataa gccatctcgg gttcaaccat
      541 ccagaattct gtcagatgcc ggcgggtctt agacttctca gcccggaaag ttggaccaaa
      601 acaataggcc ttgccaaacg cagcggcggt agcttcgtta tacagctgtc ctgattgagt
      661 gagataagcc ttttcgccct cgaaataatc aacctcaaag agagttgttg ttccttcgca
      721 ggctgccgga gtaaaaatgg gagaatcaca aagagtgaag ccgtcgttat cgagaaagtt
      781 tcgcaccgat tgaaccacgg tgtggcgaac tttcaagacc gcgtgttgac gccgggatcg
      841 caaccagaga tgacggtggt ccatcagaaa ctctgtgccg tgttccttgg gcgtaattgg
      901 ataatcgtga acttgctgca acacctgact atgtaagaca tcaatctcgt agccaccggg
      961 tgcacgacta tcggctcgga ccgtgcccct gatgatcaca gccgactcct ggcccgcagc
     1021 tttcaacgat tcccattcct cagggctaac gttgctcttc aggaccacgc attggactac
     1081 accagtgcca tcacgcagtt gcggaaagag gatcttgccg ctggaccgca tgttgtagag
     1141 ccagcccttg aggagtactt cgctaccctc gtgggctgaa agctgattga tatatgtttg
     1201 tttttcggaa gtagaagcgg atgttggcgt cgtcattgat aaaaaattac gtgggttcag
     1261 taaactcgtt cgcgcaacat acgtcgtagg gttggaaatc tctccagcca gtctgccgct
     1321 tgcagcacga ttcttaaagt tggatgaata aactgttcgt agaacagacc gcgtccacaa
     1381 acggctgtct ggtaagaaaa tgttccaatt gcttttagtc cacgctggat ggtcattaag
     1441 cgaaactctt gtgcgaattc gtccgggtcg agcaccgcga gaccctgccg gcggcgctga
     1501 tccagaaaaa acaagcggtg agcccgcaat tccgcgagcg atggcggaca aggctgccta
     1561 tccagaagga acgaaacaag gtcataagtt gcagggccca ttcgggcgtc ctggtgatcg
     1621 ataattcgca aacgggtctt accgtcaacc atgagatttg cggcgtgata gtcgcgatgg
     1681 caaagcactc gtggaaccgc ggacaactca gccgctatgt catttagctc ggctttcagt
     1741 tcagctattt ctccatgtcg caaggtgtca tggcgcagac tgccgaaata gtgttcgacg
     1801 aaaaaatcga gctcccatga cagtttggcc tcgtcaaaag ctaaccggct ggcgatggag
     1861 tgtgtttcaa cagctcgctg agtggcactt tggatctttg cgatcagccc aatagccatc
     1921 tccttataat cgtcgcacgt ctcgtcggtc tcggtatcaa agatctgaaa taactgccgg
     1981 ttccccaaat cctcctgaat gatgatccct tcgccgccat tcacttcgta aacctgcggc
     2041 accgggaggt tcgcctcaag aaagaggcgg gtaacatcga gatagggatg gaattcaggg
     2101 tcaaacggct cctgataaac cgcagccacg gcactgcctt tttgccaggg cacccgaaag
     2161 tatttccggg tggatgcgtc gggcgtcagc ggaacaattt cgtcgttcga gcctccatgc
     2221 ccagtgataa accgagctaa tcgttcggag gcgggagact caaatgcgtt agggatcacc
     2281 tgtgtagctg agctcatcag tgaattgaag tcggcaaatt ctacgaaaac cgagacttaa
     2341 acccattagc tgtggtcata ggacccgtct tgcgtggcgt cggattgtat cacccgctga
     2401 gcggcacaac aaaactttcg cctttgaaaa accccttttg ggctttttct ggaggaaccg
     2461 caccttcaac cagccttctt ggaactacta ccgcattctg aatggtgtcg cccggcaaaa
     2521 tgtgaacatt gtccgccaac actgcttttt ct
//
LOCUS       DASSFP010000457         4429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1327403, whole genome shotgun sequence.
ACCESSION   DASSFP010000457 DASSFP010000000
VERSION     DASSFP010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327403"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFH91_10320"
     CDS             complement(<1..565)
                     /locus_tag="VFH91_10320"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET6863437.1"
                     /translation="MKSDKISELTTGRLSVYLRCLNALHEAGIKTVSSQALAEQFNLN
                     SAQIRKDLGYFGEFGVRGVGYYVEDLREHITKILGLDSPHRVGIVGAGRLGTALANYN
                     GFGRSNFAVAAIFDNDSQKIGQTIGDIKLEVQDVERLGQVIRDEAIDVMVIAVPAKAA
                     QRVLNQIMSAGIKAVLNFAPISLNSRRG"
     gene            848..1471
                     /gene="ruvA"
                     /locus_tag="VFH91_10325"
     CDS             848..1471
                     /gene="ruvA"
                     /locus_tag="VFH91_10325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6863438.1"
                     /translation="MIAHLSGTLLSKHATSVILDVGGVGYEVNIPLSTFYDLGDIGAT
                     VQLRIYTHVREDALQLFGFKTARERELFLRIISVSGIGPKLGITLMSGMSADEMIESI
                     RTNNLARLTLIPGIGRKTAERLVIELREKMAALATGGVDDETQRVQSGPAPTSEDGVR
                     SDALSALLNLGYQRSSAEKAIDGVMRETPELSVESVLRSSLRKLARI"
     gene            1537..2583
                     /gene="ruvB"
                     /locus_tag="VFH91_10330"
     CDS             1537..2583
                     /gene="ruvB"
                     /locus_tag="VFH91_10330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET6863439.1"
                     /translation="MAPPEPISARVKDGSPTDEDRQLDLTLRPNRIDEYIGQRQVKEN
                     LRVYMKAARQRREALDHVLLTGPPGLGKTTLANIIANEMGAELRSTAGPAIEKAGDIA
                     ALLTNLQEGDVLFIDEIHRLLPALEEKLYPAMEDYQLDLLIGQGAAARSIKLELPKFT
                     LVGATTRAGLITAPLRGRFGIVFHLDFYPHEDLEVICKRSAEILGVAIDNEGAKEIAR
                     RARGTPRIVNRLLRRVRDYAEVDYDGRITQAVASDALNRMEVDTYGLDEMDRKLLLTI
                     IEKFNGGPVGLGTLSASIHEEKDSIEEIIEPYLIQIGFLDRTPRGRLATPLAYEHFGI
                     RFSSPNRPSLFDPR"
     gene            2605..3480
                     /locus_tag="VFH91_10335"
     CDS             2605..3480
                     /locus_tag="VFH91_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6863440.1"
                     /translation="MKRLFSFSRHSFGVRNRSPWGSMLAHALHQFHENDLFTSAAAMS
                     YFGLMALFPALLLMLATSNKIAAGSQMLTHAVDVYPGSSRFLRETINSFETLGVGAII
                     SCVVIVFWAGSWVFAVIERALNRIWGAVSRTFWHGRLLTVGMVALVGVLLSFSVMVTS
                     VLLALRDIAANLSPRQIERYPLLTSVGSAVWQVLFAVVSYLLTVVLFVLVYRFMPRAQ
                     VTLRDTLPGAFLGGLLWEVAKYIFAWSLRYFHYDQIYGSVGAVVAVLTWSYVSSLILL
                     FGAQLTAVFHREHPT"
     gene            3586..>4429
                     /gene="queA"
                     /locus_tag="VFH91_10340"
     CDS             3586..>4429
                     /gene="queA"
                     /locus_tag="VFH91_10340"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET6863441.1"
                     /translation="MLLSEFDFNLPDDLIAQEPLGKRDASRMLVLNRATQSWTDSYFE
                     AFPSHLKRQDIVVLNNSKVIPARLVGNRAATGGRVEIFLVRKLDANVWEALVRPGARL
                     RSGAQVTFGDGKLIAEILDEPGAELRKVKFSCDGLFEDVLGELGSTPLPPYIKRPSGF
                     SAYDHERYQTVYAKHNGAIAAPTAGLHFTPTVLNAVKSRANIAEITLHVGYGTFEPVR
                     VENIAEHSVSSETVEIANSAAESINTARHSGGRTFAVGTTSVRALESAVSENGQIRSG
                     NLNAS"
BASE COUNT         1057 a         1175 c         1101 g         1096 t
ORIGIN      
        1 cgccgcgtct ggagtttagg gagatcggag cgaagttcag cacagctttg attccggccg
       61 acatgatttg atttaacact cgctgcgcag ctttcgcggg aactgctatt accatcacgt
      121 cgatcgcttc atcccgaatg acttggccta acctctcaac gtcctggacc tccaatttaa
      181 tgtcgccaat tgtttggcca attttttgag aatcgttatc gaagattgcc gctacggcga
      241 aattggacct gccgaaacca ttgtaatttg ccagcgccgt tcccagacgt cccgctccaa
      301 ctattccaac gcgatgcgga ctgtcgagtc caaggatttt cgtgatgtgc tcccgcaaat
      361 cttcgacgta ataaccaacg cctctgactc cgaactcgcc gaagtatcct agatcttttc
      421 gaatctgggc agagttcaga ttgaattgct cagcgagagc ctgcgacgaa acagtcttta
      481 tgcccgcttc gtgtagagca ttcaagcagc gcaaatagac gctcaatcga ccggtcgtca
      541 gttctgatat cttgtcggat ttcatttgtg aaaacctgct cacggctcag tatagcgcta
      601 actcaatcgt ttatagaaca cttccatcca ccgattccgg cgatgccacc gattttcgac
      661 caaattggac gcgcctgata tctctcctgt ctctgcggtg atggttttct taggactttg
      721 ctcaccacag agcaacaaag gacacaaagg ttgcacagag taggctaagt taggcctacc
      781 ctaaattcca ttcgttgccg attaaatttc attcaactac aatccaaaca ccgagagacc
      841 gtcatcaatg attgcccatc tcagcggaac tctactttca aaacacgcta cctcagtcat
      901 tcttgacgtt ggaggagtgg gttacgaagt caacatccct ctttccacct tttatgacct
      961 gggtgatatc ggagccacgg tccaactgcg aatctatact catgttcgtg aggatgcact
     1021 ccagcttttt ggttttaaga cggctcgcga gcgtgagttg ttcctgcgca ttatctctgt
     1081 tagcggcatc ggccccaagt tgggaatcac cttgatgtcc ggcatgagtg cggacgaaat
     1141 gatcgagtcg atccgtacaa acaatttggc gcggctaact ctcattccgg gtatcggcag
     1201 aaaaacagca gaacgtttgg tcatcgagtt gcgggagaaa atggccgccc tggcgacggg
     1261 tggagtcgac gacgagactc aaagagtcca aagcgggccc gcaccaacca gcgaggacgg
     1321 agttcgctcc gatgcgctct cggcgctgct caatcttggt tatcaacgct cgtcggctga
     1381 aaaggccatc gacggggtaa tgcgcgaaac tccagaactt agcgtagagt ctgtgctccg
     1441 cagtagtcta cgtaaattgg cgcgcatata aagccgattt gcaactgatc tgaaaacgca
     1501 ctcgtatata ttggccttcg tggtaagtta attgtcatgg ctcctcctga accaatttca
     1561 gctcgcgtaa aggacggttc gccgaccgat gaggatcggc aactcgatct aactctcagg
     1621 ccaaatcgga ttgacgaata cattgggcaa cgacaggtca aagagaatct ccgcgtctac
     1681 atgaaagccg ctcgacaacg tcgagaagca ttagaccacg tgctgcttac cggacctccg
     1741 ggtttaggaa agaccacgct cgcgaacatc atcgcgaacg aaatgggagc cgagctgaga
     1801 tcgactgctg gtccagctat cgaaaaggcc ggcgacattg ccgcattact taccaatctc
     1861 caggaaggcg acgtcctctt catcgacgaa attcaccgac tgctaccggc ccttgaagag
     1921 aagctctacc cggcgatgga ggactatcaa ctcgacctgt tgattggaca aggagcagca
     1981 gcccgttcaa tcaagcttga attgccgaag ttcactctgg ttggagcaac tacacgagca
     2041 ggcttgatca cagctccact ccgcggacga tttgggatcg tcttccatct ggacttctat
     2101 ccacacgaag atttggaagt aatttgcaaa cgttcggccg agatccttgg cgtagcaatt
     2161 gacaatgaag gggcaaaaga gattgcccgc cgagctcgcg gaacaccacg aatagttaac
     2221 agacttttac gccgcgtacg cgactacgcc gaagttgatt acgacggtcg aattacacaa
     2281 gcagttgcga gcgatgcgct gaatcggatg gaagtcgaca cctacggtct cgatgagatg
     2341 gataggaaat tgcttctgac aataattgag aaattcaatg gaggcccggt cggacttggc
     2401 accctctcgg cttccataca cgaagaaaag gattcgatcg aagagatcat tgaaccgtat
     2461 ctgattcaaa tcggctttct ggatcgtact ccccggggcc gtcttgcaac tcccctggct
     2521 tatgagcatt tcggcatacg gttttcttcc ccaaaccgac ctagcctctt tgacccccgc
     2581 tgaacgatcc ttcggggatt attaatgaaa cgtctcttct ctttttcgcg gcattcattc
     2641 ggagtccgca atcgttctcc atggggatcg atgttggccc acgcacttca tcaattccat
     2701 gaaaacgacc tcttcacttc agctgcggca atgagttatt tcggtttgat ggctttattt
     2761 cccgcgctct tgttaatgct cgccaccagc aacaagattg cggccggcag ccagatgttg
     2821 actcatgccg tcgacgtcta tccaggatcg agcaggttcc ttcgcgagac tataaattca
     2881 ttcgaaaccc tcggagtcgg agcgatcatc tcgtgtgtcg tcatcgtctt ctgggccggt
     2941 tcgtgggtct ttgcagttat cgaacgtgcg ctgaacagaa tctggggagc agtctcaagg
     3001 actttttggc acggacgtct actcactgtg ggaatggtgg ccctcgttgg agtactgctc
     3061 tctttttcgg taatggttac ctcggtcctg ctggctctta gagacattgc cgccaacctg
     3121 tctcccagac agatcgaacg gtatccgctc ctcacctctg ttggcagtgc tgtttggcaa
     3181 gtgcttttcg ccgtcgttag ctacttgtta accgttgtgt tgttcgtgct ggtttatcgc
     3241 ttcatgccac gggcgcaagt aactttacgc gatacgttgc caggtgcgtt tctgggagga
     3301 ttgctgtggg aagttgcaaa atacattttc gcgtggagcc ttagatattt tcattacgat
     3361 caaatctacg gctcagtggg agcagtggtt gccgttttga cctggagtta tgtttcgagt
     3421 ttgattttgc ttttcggcgc tcaacttacc gcagtgtttc accgcgaaca tcccacatga
     3481 ctttggagtg cgccggctcg cggcgctttg tcacacggga tcttcgtcat atggatcttc
     3541 gaggcaccaa ctaacaaagc gccgccggac cggcgcactc caaagatgct tctctcagaa
     3601 tttgacttca acttaccaga cgacttaatc gcccaggagc ctctcgggaa acgtgatgcc
     3661 tcgcgaatgc ttgtgctcaa tcgagcaaca caatcatgga cagattcgta tttcgaagcc
     3721 tttccctcgc atctaaaacg tcaggacatt gtcgtactca acaattcaaa agtcataccc
     3781 gccaggttgg ttggcaatcg tgcggcaact ggaggccgag tcgaaatctt cttagtccga
     3841 aaactcgatg caaatgtttg ggaagctttg gtacgacccg gcgcgaggtt gcgatccggt
     3901 gcacaagtta cgtttgggga tggtaagttg atcgccgaaa ttctggacga gcccggcgcc
     3961 gaacttcgca aggtgaaatt cagttgcgac ggattgttcg aggatgtact cggggaacta
     4021 ggttcaactc ctttaccacc ttatataaaa cggcccagcg gattttccgc atacgatcac
     4081 gagcgttacc aaaccgtcta cgcaaagcac aacggcgcaa tagcggctcc caccgcaggt
     4141 cttcacttca ctcccacagt cctgaacgcc gttaaatcgc gcgccaatat tgccgaaatt
     4201 acattgcatg ttggatacgg aacttttgag ccagtacgag tggaaaacat agccgaacat
     4261 tcagtcagca gcgaaaccgt cgagatagcg aactccgccg ccgaatcgat caacactgcg
     4321 aggcacagcg gaggtcggac gtttgccgtc ggaacaacaa gcgtgcgagc gttggaatct
     4381 gcggtttcgg aaaatggtca aatcagaagt ggtaacctga acgcttcca
//
LOCUS       DASSFP010000458         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1463639, whole genome shotgun sequence.
ACCESSION   DASSFP010000458 DASSFP010000000
VERSION     DASSFP010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463639"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..765)
                     /locus_tag="VFH91_10345"
     CDS             complement(292..765)
                     /locus_tag="VFH91_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET6863442.1"
                     /translation="MNDVNQEQNTACDYRCPLDFFELTKAPIDVGSVARRVIPRECGA
                     TVTLDGYAREWTKDRKTEHLVYEAYEPMAVSEMQRIGAEAHKQFEIAHVGIVHRTGRL
                     EIGDTSVVISVSAPHRRAAFEACQWLISELKRTVPIWKKEVFADGEVWIEGDSNL"
     gene            complement(773..1042)
                     /locus_tag="VFH91_10350"
     CDS             complement(773..1042)
                     /locus_tag="VFH91_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6863443.1"
                     /translation="MSSKPDDTIIVRILFFGGARDATSASEVDLHVPTTATVKVVFEK
                     LTERFPELERFGRSLLFAVNREYAGPDVRLKGNDELAIFPPVSGG"
     gene            complement(1039..1740)
                     /locus_tag="VFH91_10355"
     CDS             complement(1039..1740)
                     /locus_tag="VFH91_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET6863444.1"
                     /translation="MSEGFDKVRGVVELPVFPLPVVLFPGVPLPLHIFEPRYQQMLKD
                     IQTTNNLFGIAYFDASSAETDLPAAGHVGCVAEVNEAQTLPDGRSNILTLGVIRYRIE
                     SYIERGDPYLVARVSYFEDQDEDDTLLVESTRDVADTFTRIAKAIRAMNDERAVLPDI
                     SDTDPQRLSFLVAAALEIEGEIKQELLELRSTSERLDRLRGMLGQAVRGYEERARIHQ
                     LAKSNGHSGKKIDIE"
     gene            complement(1984..>2418)
                     /locus_tag="VFH91_10360"
     CDS             complement(1984..>2418)
                     /locus_tag="VFH91_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6863445.1"
                     /translation="LFESKEIAPEMKSKILVVDDDKAITQQLYWSLCNEYDVITANDM
                     QTAIRRAMFYKPDVSILDLQMPPEEDTPAVGMRLLEYLKGNLPDSKVLIMSSNNDQGV
                     QRACYAAGADEFFDKPFEVEHMLASICRLMPVHRLELFAQIL"
BASE COUNT          601 a          621 c          597 g          599 t
ORIGIN      
        1 ggaagccgca gacattcgca agactggccg tagcgcccag ggagtcaggt taataaagac
       61 tgacgcctcg gataaggtta caagcgcgtc gctggttgag gctgctgcag aagatgagac
      121 ggaggaaacg ccggcgggtt aggtagggat tattggtcat gtctctgcgt ccctctgcgt
      181 tgaactcaga accgagcatt atcgcaacgc agagagacgc agagatcttg cagagggacg
      241 cggagatcag tgataactat gaaactattg ggtatcaacg gagtgccgcc actacaaatt
      301 tgagtcgcct tcgatccaga cttcaccgtc agcaaagacc tcttttttcc agatcgggac
      361 agttcgcttc aattcagaga tcaaccattg gcaggcttca aacgctgctc gccggtgagg
      421 cgcgctaaca ctgatgacga cgctcgtatc accaatctct aaccgtcccg ttcggtgaac
      481 aattccgaca tgagcaatct cgaattgctt gtgagcttct gctccaattc gctgcatctc
      541 actgactgcc atcggctcgt aagcttcata gaccaggtgt tcagtcttac gatctttggt
      601 ccattcccgc gcgtatccat ctaacgtaac ggtcgcaccg cattctcgag ggataactcg
      661 cctcgcaacc gaaccaacat caatcggcgc cttcgtcagc tcaaagaagt cgagagggca
      721 tctgtaatca cacgcggtat tttgttcttg attgacgtcg ttcatcgcac tgtcatcctc
      781 cgctcacagg agggaatatg gctagttcgt cgtttccttt caacctcaca tccggtccgg
      841 catattcgcg atttacagca aatagtaacg agcgaccgaa tcgctcaagt tcaggaaagc
      901 gctcagtcaa tttttcgaaa acaaccttta ctgtggccgt cgtcggaacg tgtaagtcta
      961 cctcgctggc ggacgtggca tctcttgcgc ctccaaagaa caggatgcgt actatgattg
     1021 tgtcatcagg tttagagctc attcgatgtc aatcttcttc ccgctatgtc cattgctttt
     1081 cgcaagttga tggatgcgcg cacgttcttc gtaccctctt accgcctgtc ccaacattcc
     1141 tctgagccta tcgaggcgtt cagaggttga acgcagctcg agtaactctt gcttaatttc
     1201 accttcgatt tcaagggctg ccgcaaccag aaacgagagt cgttggggat cagtatcaga
     1261 aatatcaggt aagacggccc gttcatcgtt catcgcgcga atcgctttgg ctatgcgagt
     1321 gaaagtatca gctacatctc gggtggattc aacgagtaaa gtatcgtctt catcctgatc
     1381 ctcgaagtag ctgacgcgcg ctacgagata aggatctcca cgctcgatgt acgactcgat
     1441 tcgataacga atcaccccga gcgtaagaat attcgatcgg ccgtctggaa gagtttgagc
     1501 ctcgtttact tccgcgacgc agccgacatg gccggcagct ggaaggtcgg tttctgccga
     1561 gcttgcgtca aagtaagcaa ttccaaaaag attatttgta gtttgaatat ccttgagcat
     1621 ctgctggtaa cgcggctcaa agatatgcaa cggcaagggc actcccggaa aaagcacgac
     1681 gggcaacgga aacaccggga gttcgaccac tcccctgact ttatcgaatc cttcgctcat
     1741 gctgagatgt tagcggctgg gggtaagtta cgaccgaaat ttttagatca ggaaaggtcg
     1801 cattatcgca gacagaggaa cataagtcag tctgagattt gctatttcag atctcgagtc
     1861 aaaaaagagc ggccccaact ccgcgaaggg gactcggagg tggggccttt acacaccttc
     1921 tgatgaagtg ggattcatct ccggtgttct aagtgaacac ttaaagggtg ggtagtgttc
     1981 aattcaaagg atctgggcga acagttccaa cctatgaact ggcattagcc tgcaaatact
     2041 tgccaacatg tgctcaactt caaatggctt gtcgaagaat tcgtcggcgc cagccgcata
     2101 acaagctcgc tgaacaccct ggtcattatt cgaactcatg attaagactt ttgaatcggg
     2161 gagattaccc ttgaggtact ccagcaggcg cattccaaca gccggggtgt cttcctcagg
     2221 cggcatctgt aggtcgagta ttgaaacatc aggtttgtaa aacattgccc gacgaatcgc
     2281 agtctgcata tcgttcgcag tgattacatc gtattcattg cacaacgacc aatagagctg
     2341 ctgcgtaatg gccttatcgt cgtcaactac taaaatcttc gacttcattt caggggcgat
     2401 ctccttgctt tcgaacaa
//
LOCUS       DASSFP010000459         3778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1031596, whole genome shotgun sequence.
ACCESSION   DASSFP010000459 DASSFP010000000
VERSION     DASSFP010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031596"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFH91_10365"
     CDS             complement(<1..217)
                     /locus_tag="VFH91_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863446.1"
                     /translation="MTATRVCFMLASLLVSIAFAGNQEQIVQVKPDGDWKGTLDVAGT
                     RLDLVLHISSKDGVLAATLDSPDQGATG"
     gene            complement(232..1908)
                     /locus_tag="VFH91_10370"
     CDS             complement(232..1908)
                     /locus_tag="VFH91_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6863447.1"
                     /translation="MKLKSINLIAAFIWLAAAIVSSIQAQEPVPAKNPGSISGRITVD
                     GKPKAGLVVELLATDTNGPRRPIAKATTSKTGKYVLTNVESGTYDVSPTAPTLVVPNQ
                     GRAGQSGISLTIEAGERLKGIDFDLISKGSIRGRVRDLSGEPVKGQTVELILRGADNY
                     SRPFYPSAPDELKTNAEGAYRISGVPPGRYILKVGVAFGLAAFGGSEKGHVYYPETFY
                     PSADEESKATVVEVTAGRETPDIDITVNNPLKIYEIVGQLIADETGAPVPNVNLEIIT
                     TSATGGRSSHLGGAFKSNANGEFRIPNASPGHYVVAAANDPVSNTYGDPVSFDVKDED
                     VTGLRIPMHSASIMTGVVTIEGSVDPAIGNMPPMLMITAATLSTELMGSSVTANVNPD
                     GGFRLTGIRPGKVSLSSFIHRGKPGPLRILRIERNGIELRDSIDVRPGEDISGLRLVL
                     GSGSSVLRGEVKIEGGPLVGVDLSVLYRSINGDAQRFYRAELDARGHFVIKGLIPGEY
                     ELTIGPMSVEISGEGGSRMMNRMPTVKQNVVVGAGADAFVTLVMTLKPEN"
     gene            2076..>3778
                     /locus_tag="VFH91_10375"
     CDS             2076..>3778
                     /locus_tag="VFH91_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6863448.1"
                     /translation="MSRNISPLLLGCLIIPLTAFFSTPAHSQSRTLAKVVIKVSLSDN
                     LGQNFGLVIKKLDNESAISAIRVKTSADGSVSVSLVPGDYVVESEKPLIVKGKSYEWS
                     VKLKVESSTALLELNNENATITAADEALRRGRIVDETDLLKVLSDGVVNVQGELAQGT
                     GSIVDSTGLILTSQHVIERSKELRVQFGDNKKVAAKLVAEDPDLDLAILWVDLSACPS
                     CKALASGSNTVVDGSGVFTISSLLAQDKTLTKGTLRTTGDGINTDFKIASVDSGAPVF
                     NRLGEVIGVLRFVDQNGARQGTSGVIRIQDSQGLLAKARQIVKEALAFPSPELLPTEP
                     ADSYPAEDLKNLDLKKFKPRAYEADFGEYQLTMITPVLKHYLIEQNRIAFERQRKKIE
                     KDSVITSPVMANSFYQLRNWSDYINQMRPVVHLLIVPEVSATGKSTFFSLLTLGVGLM
                     SGGAMVLPPDFKFKADFEEMSLTCDGKPITPIQRGKIEFIKSLTSYLKMKQRTAYAGI
                     YTYSADAFAPDKCKQMSLKVVSQQNPTAPQIRVVDPRMVQQVWSDFEPYRQQAGKHFD
                     VR"
BASE COUNT          914 a          973 c          984 g          907 t
ORIGIN      
        1 atccagtcgc cccctgatcc gggctgtcga gcgttgcagc caacacgccg tccttactgc
       61 taatgtgtag gaccagatcg agcctggtcc cagccacatc aagagtgcct ttccaatctc
      121 cgtccggctt cacctgtact atttgctcct ggtttccagc gaaagctatc gacacgagca
      181 agcttgcgag catgaagcaa actcttgtgg ctgtcataaa gtttttcatt tttagttttc
      241 gggttttaga gtcattacca gggttacgaa agcgtctgct ccggctccaa ccacaacgtt
      301 ttgtttcaca gtcggcatcc gattcatcat gcggcttcct ccctcacctg agatctctac
      361 actcattggt ccaatcgtta gctcgtattc gccgggtatc agacctttga tcacaaagtg
      421 cccgcgcgcg tcgagttcgg cgcggtagaa cctttgtgca tcgccgttta ttgagcgata
      481 cagtacagat agatctacgc ccaccagtgg accaccttca atctttacct ctccgcgcag
      541 cacgctgcta ccggagccaa gcactaacct gagtccgctt atgtcttcac caggacgaac
      601 gtctatactg tcgcgcaatt caatgccatt gcgctcaata cgcaagatcc ggagtggccc
      661 aggcttgcct cgatggatga aagaggacaa acttacttta ccaggacgaa ttccagtgag
      721 acgaaagcca ccatcagggt tgacattcgc agtcacggat gaacccatca gctcggtaga
      781 caacgtggcg gcggtgatca ttagcatggg cggcatgttc ccaatagcag gatcgacact
      841 tccctcgatt gtgacgacac cagtcatgat cgaggcggaa tgcattggaa tcctcaagcc
      901 ggtaacgtcc tcatctttaa cgtcgaacga aactggatct ccataagtat tgctcactgg
      961 atcattcgca gcagccacaa catagtggcc cggcgacgcg ttcggaattc tgaactctcc
     1021 attggcgttg ctcttgaaag cgccgcccag atgactggaa cgcccaccag ttgcactcgt
     1081 ggtgattatc tctaaattaa cattcggtac aggggcgccg gtctcgtcag cgataagttg
     1141 gcccacgatc tcgtagatct tcagaggatt gttaaccgtt atgtcaatgt cgggagtttc
     1201 gcgtccagcc gtaacctcga caactgtcgc cttggattct tcatcggcgc ttgggtagaa
     1261 agtttccggg tagtaaacgt ggcccttctc gctccctcca aaggcggcga ggccgaacgc
     1321 gactcccact tttaagatat accgacccgg aggcacgccc gagattcgat aagcaccttc
     1381 cgcgttagtc ttaagttcat ctggtgcaga cgggtagaag ggacgcgagt aattgtctgc
     1441 accgcgaagg atcaattcca ccgtctgacc ctttacaggt tccccactaa gatcccggac
     1501 ccgcccacga atgcttcctt tgcttatcag atcgaagtcg attcctttaa gcctttcgcc
     1561 cgcttcaatc gttaagctta tgccggactg gcccgcccgg ccttgattgg gaacgacaag
     1621 agttggtgcg gtaggagaaa catcgtacgt tccggattca acgttggtta gcacgtactt
     1681 accggttttg ctggtagtgg ctttggcaat cggacggcgt gggccattgg tgtctgtggc
     1741 cagtagttcg actaccagac ctgctttggg ttttccatct accgtgatac gcccggagat
     1801 agagcctgga ttcttagcag gcacaggttc ttgagcttga atgctgctaa ctattgcggc
     1861 cgcgagccag atgaaagcgg caatcaggtt gatcgatttg agcttcattt cggttacctc
     1921 ccgtgagacg caagcagcca acgcttattc aatcatacgg actggtttgc agaaagcttc
     1981 gacgactggt attcaagtgt ccgataaact ttggtttgtc ggcacaactc gacaagctaa
     2041 agcttatcgg acatctctcc gaatggagtt aatcaatgtc gcgaaatata tcccccttgc
     2101 tactcggttg cctgatcatt ccgctcacgg cgttcttctc gactcccgct cactcgcaga
     2161 gccggactct agctaaggtc gtgatcaaag ttagcctgag tgacaatctg ggccaaaact
     2221 ttggcttagt gattaagaag ctggataacg agtcagcgat ttccgccatt cgagttaaaa
     2281 cgtcggctga tgggtcggtc tctgtttcgc tggtaccggg cgattacgtt gtcgagtcag
     2341 aaaaaccact catcgttaag ggaaagagtt atgaatggtc cgtcaagctg aaggttgaat
     2401 ccagcacagc cttgctggag ctgaacaatg aaaatgcgac gatcacagcg gcggatgagg
     2461 cgctgaggcg agggcgaatt gttgatgaaa ccgatctgtt gaaggtcttg agcgacggcg
     2521 tggtaaacgt gcaaggtgag ctggcccaag gaacgggatc gattgtcgat agcacgggat
     2581 tgattctcac cagtcaacac gtgatcgaaa gatcgaagga gctgcgagtt caatttggcg
     2641 acaacaaaaa agtagcggcg aagctcgttg cagaagatcc cgatttagat cttgctatct
     2701 tgtgggtaga cctgtccgcc tgtccttcct gtaaagcttt ggcgagtgga agcaacacag
     2761 tcgttgatgg atcaggagta ttcacgatta gcagcctttt ggcccaggac aagacactca
     2821 ctaagggcac gctaagaacc acaggcgacg gcatcaatac ggattttaag attgcgagtg
     2881 ttgactcagg ggcgcctgtt ttcaatcgtc tgggtgaagt gattggcgta ctcagattcg
     2941 tggatcagaa cggggcaagg caaggaactt cgggagtaat cagaatccag gattctcaag
     3001 gcctgctcgc aaaggcgaga caaatagtaa aagaagctct cgcgttccct tcacctgagc
     3061 tgctccccac cgaacctgcc gactcttatc ccgcagaaga cttgaagaat ctggatctca
     3121 agaagttcaa gccaagagct tacgaagcag acttcgggga atatcagttg acgatgatca
     3181 cacccgtgct gaagcactac ctcatcgagc agaaccgaat tgcgttcgag cgccagcgga
     3241 agaaaatcga aaaggacagc gtgattacct cgccggtgat ggcgaatagc ttctaccagc
     3301 ttcgcaactg gagtgattat atcaaccaga tgaggccggt ggtgcatttg cttattgttc
     3361 cggaagtgag cgctacgggg aaatctacat tttttagcct gctaacgttg ggagtcggac
     3421 tgatgagcgg cggggcaatg gtcctgccac cggattttaa gtttaaggcc gactttgaag
     3481 agatgtcgct gacgtgcgat ggcaaaccga ttacgccgat tcagcgagga aagattgaat
     3541 tcatcaagag cctgacaagc tatctcaaga tgaagcagcg taccgcttat gcgggcattt
     3601 atacatactc agcagatgcc ttcgcccctg acaagtgcaa gcagatgagt ctgaaggttg
     3661 tttcccaaca gaatcccact gctccccaga taagagtagt tgacccgcgg atggtccaac
     3721 aggtgtggag cgattttgaa ccctatcgtc agcaggctgg caagcacttt gatgtacg
//
LOCUS       DASSFP010000460         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_139135, whole genome shotgun sequence.
ACCESSION   DASSFP010000460 DASSFP010000000
VERSION     DASSFP010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139135"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /locus_tag="VFH91_10380"
     CDS             complement(<1..2259)
                     /locus_tag="VFH91_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863449.1"
                     /translation="MNNRKRIAVSSNTAARLQAAAQWTESQLNSSELLIISHSIDAAN
                     NFCLSVASGREALFGTRRLTLNGLAARLAQRSLAQSETVPATALSFNGIVARAIYLLN
                     AQDKLTYFAPVATKPGFPIAVARTLEELRMNDVRPDTLASLPGGGKDLGEIARVVEAE
                     LDDSKIGDRAKIFQAAIASVSSLHNSDYVRVPTLLLDVCIRSQLEFDLVRALVEISPD
                     VLITAPYGDERTIGLAELASEQQRDVIQVADGPVTSLTRLQEFLFESPPSVAELDETV
                     DLQNWPGEPRECIEIVRSIQLEAARGVPFDQIAIFLNSPGEYRSFLEEAFRRAHIPAY
                     FAHGSTAPDPAGRALLALLSCAADGLSAGRFAEYLSLGQVPDLVRNNDADLLWEPPEN
                     DLLPVSETDERVDTNSTQKSKSLPLFDEPERRTVIEGALRAPSQWEKLLVDSAVIGGK
                     DRWERRLKGLEKELTLRLEEVSPEEEAQVESIQKQLRDLSSLSEYALPLIKRLSEFPE
                     KANWGTWLRHLRELAVNALRQPEEVLATLAELEPMSPVGPVDLYEVQLVLGPKLRDLS
                     VQPRQRRYGCVFVGTADLARALSFQIVFVPGLAERIFPRKVVEDPILPDEQRRQSGSD
                     TLNLRKDRLELERLSLRLAVGAARERVYLSYPRIDVQQSRPRVPTFYALETLRAAEGV
                     LPGLEEISARAESKTRARLGWPAPERSADAIDEAEYDLALLANLLTQDADEAIGSAHY
                     LLSANEHLARALR"
BASE COUNT          573 a          633 c          652 g          581 t
ORIGIN      
        1 cctgagtgcg cgggcgaggt gttcatttgc tgacaaaaga tagtgggcgc tgccgattgc
       61 ctcgtctgca tcctgggtaa gtagatttgc gagtaaggcc aggtcatatt cggcttcgtc
      121 gatcgcatct gctgaacgct cgggtgcggg ccagcccagt cgcgcgcggg ttttcgattc
      181 ggcccgcgct gaaatctctt caagtcctgg caacacacct tccgcggccc gcagagtttc
      241 gagcgcataa aatgtaggaa ctctcggtcg cgactgttga acgtcgatgc gcggatacga
      301 gagatagact cgttcacgcg cagcaccaac cgcgagcctc agtgataacc tttccaactc
      361 aagtcgatct tttctgagat ttaatgtgtc tgagccactt tgtcgtcgct gctcatccgg
      421 caggattgga tcttcaacaa cttttctggg gaagatgcgc tctgccaatc cgggaacaaa
      481 gacgatttgg aatgaaaggg ctctcgccag atctgctgta cccacgaaga cgcagccgta
      541 tcgacgttgc cgtggttgaa ccgaaagatc gcgaagcttg gggccaagaa cgagttggac
      601 ttcatacaga tcgactggac caactggact catcggctct aactctgcga gagtggccaa
      661 cacttcctcc ggctgtcgca gggcattgac tgctaactct cgcaggtgtc gcaaccatgt
      721 tccccagttt gccttttcgg gaaactctga aagacgcttg atcagcggaa gagcgtattc
      781 actaagactg cttagatccc gcagttgttt ctgtatcgac tcgacttgtg cctcctcttc
      841 gggagacacc tcctccaatc gcagtgtcaa ttctttttcg agccctttta gtctacgttc
      901 ccagcgatcc ttgccaccga ttacggctga atcgacgagt aacttttccc attgtgaagg
      961 agcacgcaaa gctccttcga ttaccgttcg tcgctctggc tcatcaaaca aaggtagtga
     1021 tttagatttt tgtgtcgagt tcgtatcaac ccgctcgtca gtctcggaaa ctggcagtag
     1081 atcattctcg ggtggttccc acaaaaggtc ggcatcattg tttcttacaa ggtcaggtac
     1141 ttgtccgagc gagagatatt cagcgaatcg accggcggat agcccatctg cagcgcaaga
     1201 aaggagagcc aacaacgctc gccctgcagg atctggtgca gttgatccgt gcgcgaagta
     1261 agcggggatg tgtgctcgcc tgaacgcctc ttctaaaaag gaccggtatt caccgggcga
     1321 gttcaggaag attgctatct gatcgaaagg aaccccgcgc gcggcctcaa gctgaatgct
     1381 tctaacgatc tcaatacact cacgaggttc accgggccaa ttttgcagat cgaccgtctc
     1441 atctagttcg gcgactgacg gtggtgattc aaaaaggaac tcttgcaatc gagtgagcga
     1501 ggtgactggt ccatcggcga cctgaatcac atctcgttgc tgctccgatg cgagttcagc
     1561 caatccaatt gtcctctcgt ctccatacgg ggcagtgatg agaacatcgg gcgaaatctc
     1621 gacgagggcg cgaaccaggt caaactccag ctgactccgg atgcagacat ccaaaagtaa
     1681 tgttggtact cgaacgtaat cggagttatg aagtgaggaa accgaagcga tggccgcctg
     1741 aaagatttta gcgcggtcac cgatctttga atcatctaat tccgcttcta caacgcgggc
     1801 aatctcgcct aggtccttac cacctccggg caaggacgcg agcgtatccg ggcgtacatc
     1861 attcatcctc aactcttcga gagttcgtgc gaccgcaatt ggaaagccag gcttagtcgc
     1921 taccggagca aaataggtga gcttatcctg cgcattgaga agatagatcg ctctggcaac
     1981 gattccattg aatgagagcg ccgtggcggg aaccgtttcg gactgtgcca gggagcgttg
     2041 tgccagtcgc gccgcaagac cattcaaagt caaccgccgt gttccaaaga gagcctcacg
     2101 accgctggcg acggataaac aaaagttgtt ggcagcgtca attgagtgac tgatgatcag
     2161 cagttcggag ctatttaatt gagactcagt ccactgtgcg gcagcttgaa gacgtgctgc
     2221 cgtgtttgag gaaactgcta ttcttttgcg attgttcatt tactacccga gcattatgta
     2281 agggaagcca ggagtatacc aacgcagtgt tcctaaaacg gaatgtttag tggatttatt
     2341 ttaggatggc ggcctgcgca ctcaagctca gaaggtgcca gcgctgaaaa gtgttgattg
     2401 cttcgttggg catcaggcgg acaaatgcag tctgaaaat
//
LOCUS       DASSFP010000461         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1634426, whole genome shotgun sequence.
ACCESSION   DASSFP010000461 DASSFP010000000
VERSION     DASSFP010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634426"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="VFH91_10385"
     CDS             complement(<1..1994)
                     /locus_tag="VFH91_10385"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6863450.1"
                     /translation="MHEAREAIINRLAMTLPGSINLDRFLNVIVSELGRMMNVDRCDV
                     IQLTSASELRISHEWRASSDVPSSLGTVIPLELKQLSDHVDIRKPIRLNDTAASGLDQ
                     KVRFLAASLGTRSLLVVPVTLGNDVLGLVGLHTTRTPREWLDDEVSFLQSIAGQIAVG
                     YQYTRLYTDKEREAETKRALLSIANALNARSDFREVSTLVLERAIALVGADYSALGVL
                     DQNGSRISLAAFKAAPDAAIDSVQGLFDAHGKSLDVTAFPAMVEVIGQGKTLQLLETD
                     LPFPIRFMFNSILGGRAALVAPVRVGGQTFGLLGLVWSVAREGFQEHEVALVEGIADQ
                     IGTALERDQLSAEIMHLRSALHERYGEERIIGQTQTIRRAIELALSVADTQTTVLIEG
                     ESGTGKELLANLIHYNSGREDQPYVKLNCGAIPETLLESELFGHEKGAFTDARALRRG
                     RFEEADSGTLFLDEVGEMSLSAQVRLLRVLQDGEFTRVGGNEVIKTDVRVLAASNVDL
                     KRAVELGSFRQDLFYRLSVFPITLPPLRERPEDIHPLVIHFIEHYKQKTGRFIAGISK
                     DALQALITYNWPGNVRELENAVERAVIIASGRQIELEDLPEAITKIALQERDRIKVER
                     AKAASEGRTSTFEVTVPSSMEEIERQAIEATLDYTGGDKS"
     gene            complement(2189..3118)
                     /locus_tag="VFH91_10390"
     CDS             complement(2189..3118)
                     /locus_tag="VFH91_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6863451.1"
                     /translation="MRVLFSSSTSIFKLTNFRLFITTLLVASTITLTVLAQSGVRPSA
                     NTAGGVSVNVVARREDNRNNPITSKEVSVYDNGVEQSIKNFTLDPSPARIVLLVDNSL
                     SIRADVEKLEAAAREFAYEIYQGDKLLIVGYDEEAEIVADWTDDAKSIESSLKLFRKK
                     GQPHLFDAIRAVVEQAMRPLMGQKRIIVVISDGLDRGSKATFEDTLAELQSENIMVYA
                     VQAVDRTRGAIRRDVPKPKVVIDKLAEGTGGLIYSIDQPTDAAKGICDELRKNRYVLS
                     YTPSSAPFGQARSLLVLGNQGITIRAKSMQQPN"
BASE COUNT          793 a          898 c          852 g          901 t
ORIGIN      
        1 tgagatttat cgccgccggt gtaatcaaga gtcgcttcaa ttgcctgccg ctcgatctcc
       61 tccatcgacg acggcacagt cacttcaaag gtcgaggtgc gaccctcgct cgcagcctta
      121 gctcgctcca ctttgattcg atcacgctct tgcaaggcaa tcttcgtaat tgcttctggt
      181 aagtcttcca gttcgatctg gcgtccagac gcaatgatca cagctcgttc aacagcattt
      241 tcaagctcgc gcacattgcc gggccagttg taggtgatta aggcctgaag tgcatccttt
      301 gagattcctg cgataaatct acccgtcttc tgcttgtaat gttcgatgaa atgaataacg
      361 agtggatgga tgtcttcggg ccgttcgcgc agcggcggta gagttattgg gaaaactgaa
      421 agccggtaaa aaagatcctg gcgaaaactt cctaattcga ccgcccgttt tagatcgaca
      481 ttcgacgccg ctagcactcg aacatccgtc ttgatcactt cgttaccacc aacgcgcgta
      541 aactcaccat cttgcaatac ccgcaataag cgaacctgcg ccgacaaact catttcgccg
      601 acttcatcca ggaacaacgt gccactgtcc gcttcttcaa atctgccacg acgcagcgcg
      661 cgcgcgtcag taaaagctcc cttttcatgg ccaaacaatt ccgactcgag cagggtttcg
      721 ggtatcgctc cacagttaag tttgacgtaa ggctgatctt ctcgaccaga gttgtagtga
      781 atgaggtttg ccagcaactc ttttccggta cccgactcgc cttctatcaa tacagtggtt
      841 tgagtgtcgg ccacactgag cgcaagttcg attgcacgtc taatcgtttg tgtctgtccg
      901 atgatgcgct cttcaccgta gcgctcatgc agagcgctgc gaagatgcat gatttcagct
      961 gacaactgat cacgttcgag cgcagtccca atctggtctg cgatgccttc gaccagcgca
     1021 acttcatgct cctgaaatcc ttcgcgagcc acgctccaaa caagccccag cagtccaaat
     1081 gtttggccgc caactcgcac cggcgctacg agagctgctc gcccgccgag gattgagttg
     1141 aacatgaatc tgattggaaa aggcagatcg gtttcgagaa gttgcagtgt cttgccttgt
     1201 ccaatcactt cgaccattgc cggaaatgcg gttacatcaa gggattttcc gtgcgcgtca
     1261 aacaggccct gcactgaatc aatagctgca tccggagccg ccttgaaagc agcaagactg
     1321 attcgacttc cattctgatc gagcacgcca agcgccgaat aatctgcgcc cacgagggcg
     1381 attgcgcgct caagcacgag agttgaaacc tccctgaagt cagagcgtgc gttcaatgca
     1441 tttgcgattg agagaagcgc acgttttgtt tcagcttcgc gctccttgtc cgtgtagagt
     1501 cgagtgtact gatagccgac cgcgatttgt ccggcaattg attgcaggaa tgaaacttca
     1561 tcgtcaagcc actcgcgagg tgtacgagtc gtatgaagac caacgagacc aagtacgtcg
     1621 ttcccgagcg ttactggaac caccagcaaa gaacgtgtcc caagactcgc ggcgagaaac
     1681 cttacttttt gatcgagacc cgacgcggcc gtgtcgttga gacgaattgg cttgcgtata
     1741 tcaacgtgat cagagagttg ttttagttcg agtggaatga ctgttcccaa actcgagggc
     1801 acatcgctcg aggctcgcca ctcgtgactg atgcgcagtt cgcttgctga tgttaattga
     1861 atgacgtcgc agcgatcgac attcatcatg cggcccagtt ccgagacgat aacgttcagg
     1921 aagcggtcaa gattgatact gccgggcaaa gtcattgcga ggcgattaat aatcgcctct
     1981 cttgcctcat gcatttttat ctttcgttca agcgaaagag attgcgcgct ttcagacatg
     2041 gtttcggatt aatggatact tctcatttgg tatttgataa ttgttaattg gtttttgcaa
     2101 ctcccgaacg tggatcgcca tcggctggaa ggttggcccc taacgaccac accttaacat
     2161 ttaacaattg tcaaatgata attcgatctt agttgggctg ctgcattgac ttagcgcgga
     2221 tggtaattcc ctggttgccc agaacgagga gacttcgtgc ctggccaaag ggagcgctgg
     2281 atggtgtgta cgacagcacg tagcggtttt ttcgcaactc gtcgcaaatt cccttcgccg
     2341 cgtctgtagg ttggtcgatc gagtaaatca gacctcccgt accttctgcc agtttatcga
     2401 tcacaacttt gggcttaggc acatcccgac gaatagcgcc acgcgtgcga tcaacggctt
     2461 gcactgcgta gaccatgatg ttttcagact gcagttctgc aagtgtatct tcaaacgtag
     2521 ctttactgcc tcgatcgaga ccgtcgctga tcacaactat aattcgtttc tgacccatca
     2581 acggtcgcat cgcttgctca actaccgcac ggatagcgtc gaacagatgt ggctggccct
     2641 ttttgcgaaa caattttaag gacgactcga tcgactttgc gtcatcggtc caatcagcaa
     2701 cgatttccgc ctcttcgtca tagccaacaa tcaaaagttt atcgccttga taaatctcat
     2761 aggcaaattc gcgtgctgcg gcttcaagtt tctcaacgtc agcccgaatt gagagcgaat
     2821 tgtctaccag gagtactatg cgcgccggcg acggatcgag tgtgaaattc ttgatcgact
     2881 gttcgacacc gttgtcgtag acggatactt ccttactggt gattggattg ttgcgattat
     2941 cttcacgtct ggcgacgacg ttcacgctca ctccgccggc ggtgttggcg cttggacgaa
     3001 caccggactg ggccaagacg gtgagagtta tggtcgaggc tacgagcaaa gtggtaatga
     3061 acagtcggaa attcgtgagt ttgaaaatgg acgttgaaga ggaaaataac acccgcatgc
     3121 tttaaatcgg cctaatccgt acctgacttg gacggcgacg gtttgtcggt ctttttggcc
     3181 gaagagctct ctttggtttt agtttcagtt ttcttgtcgg acgatgactc ggattccgac
     3241 ttctctttgg aagttgcctt gccggcatag tcggtaacgt accagcccga acccttaaac
     3301 tgaattgccg gggcggaaat aagtcgttct agtttaccgc cgcattttag gcactttgct
     3361 gggggtttat cgttaacctt ctgaaagact tcacttttgg ttttgcattt ctgacattcg
     3421 tattcgtaaa tcggcattta atcg
//
LOCUS       DASSFP010000462         5600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_2084116, whole genome shotgun sequence.
ACCESSION   DASSFP010000462 DASSFP010000000
VERSION     DASSFP010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084116"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFH91_10395"
     CDS             <1..274
                     /locus_tag="VFH91_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6863452.1"
                     /translation="IGHPERDRDKLRRISPLFHAERISSPLLVLHGANDPRVPKIQSD
                     RMVEAVKRNGGIVEYLVFHDEAHGLRQPANVAKAYTAILDFLDRYL"
     gene            complement(257..928)
                     /locus_tag="VFH91_10400"
     CDS             complement(257..928)
                     /locus_tag="VFH91_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863453.1"
                     /translation="MKVILFGASGMVGQAVLRECLLDKSVERVLSIGRRASRQSHEKL
                     REIILPDLFDFSIDQAELKAFDACLFCLGVSSVGMDPAEYKRLTFDLTMGWANLLARG
                     NPRMTFVYVSGAGTGGTAKWAQVKGRTENELLKLFPSSYMIRLGALRPMHGEVSKTRW
                     TRVGYALVRPILPLIAAVSPSAIISSEELGRAMLQLAKEGAPKRILGNSELRTLLQHT
                     SKVSI"
     gene            complement(1112..1336)
                     /locus_tag="VFH91_10405"
     CDS             complement(1112..1336)
                     /locus_tag="VFH91_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863454.1"
                     /translation="MRKLKLIAISVCLLAGLMFAIPNPVQPAPIPQGASQSPKGGPLP
                     PPPPTPTPETIAPPSNSSSLLGKLLKAIVG"
     gene            complement(1428..3572)
                     /locus_tag="VFH91_10410"
     CDS             complement(1428..3572)
                     /locus_tag="VFH91_10410"
                     /inference="COORDINATES: protein motif:HMM:NF033523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Atxe2 family lasso peptide isopeptidase"
                     /protein_id="HET6863455.1"
                     /translation="MTKLRKTAIRIVETVGPSLGHRWRTALPNLFAWSVGVVFLSASI
                     CSGQSELSAHGFDPTPVKLGKFEKAPKRAITSLDLLQMRRVLGVKISPDGKSIAFVLV
                     QALVATNSYRTGLFVASTSDKNKVAEFGSAGPPEFDSLGQLLSIAPQWSPDSSYVTLC
                     LPSNGVRQITRWPREGGPPEQLTRSQHDVQTYEWSKDGKEIVYRTVAPIDLTAVRTLA
                     QQGLVYDGTIRASRAKPVTELLRERQSRAMDWWKYSVSTGHERRLSSDEIKQQQADQN
                     IGTTDTGVYVARRSPDGKSTAYIKRLQSPQDFKYYGWSLINQDYSGRRIILVPATNAL
                     ISDIHWSNTGDKIYFAEVSVNGAGLFEVPASGGPIRRLIRSVDPLSNFSFDASQQSVA
                     CVIENTISPPNIALIRVSDRRVVTLTDLNPEFQTLNLSAPSRLDWSNEYGDAAFGYLF
                     KPLDFDSNKSYPLIITTYTAGGFLSGAVGDEFPIQVFAAHGFLVLAFNAPDRRPADPG
                     DFKTQMLTWYSPLSSLEVVVRKLVREELVDPNRIGLTGLSYGAEITEFAITHSSMFAA
                     AATGGWSARDPIFYYLAHDGWRRIFTDWMGSGPEGKGQNKWRELSPALNVTNLTAPLL
                     VQAAESEYLCGLQLYTTLKEREVPVELIIYPNEGHVKNQPAHKYFVYERNLAWFKFWL
                     LEKTPKQKSSWSKEWSEFKEKSVSPPALASRN"
     gene            complement(3585..>5600)
                     /locus_tag="VFH91_10415"
     CDS             complement(3585..>5600)
                     /locus_tag="VFH91_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863456.1"
                     /translation="DHSFNARFTLFGRYNYAPSESLSRAGDGLRSLNSIRVFSNRTQT
                     LTVGATNSFSATTFNELRFNFSNVFGKSWSRLDTFGGAAVPAESLLFPSFVSFSDAFF
                     RLSITGGRNASIQAGTNVSNRQRQFNIVDSLSAVIGSHQLKFGVDYRLLLPVIGIRGY
                     AQTIQFGNVTNAIASLASSVQVDAQTGPQELRYTNLSLFAQDAWRAHRRLTLTYGFRW
                     EVNPPPTELNGNFAFAVTGVENIPTMTLAPRGTALYQTTYNNFAPRVGVVYQLMDEPG
                     NELILRGGAGVFYDLGNAQTSNAYGASFPFAAVRRFTNTTFPISPAQASPPPITTNLP
                     VTSTIVIFDPELKLPYSLQWNLALEKSLGAHQTLSATYLAALGRRLLRQDALFNGASN
                     NPVFASTTSFTRNSASSNYQALQVQFQRRFSRGMQALVSYTWSHSIDIASNDSSANVP
                     VEVVNISQDRGDSDFDVRHSASAALTYNLPVKSSIALHGLLKDWAIDSIFSARSATPV
                     NAYFTTIAPFGIYQLRPNRRSDAPLYIEDKAEAGGRRVNPAAFVVQTPTSQGTLGRNS
                     VRGYPFWQLDFALRRQFRLAERFDLQVKGELFNAFNHPNFGDPRFEDMSLGLASGGQF
                     FPNAAFGRSTSMLGRSLASGGTGTGGLNPLYQIGGPRSIQLSLKAQF"
BASE COUNT         1402 a         1403 c         1439 g         1356 t
ORIGIN      
        1 aatcggacat cctgaaagag atcgcgacaa gctccggcga atctctccgc tatttcatgc
       61 ggaacgaatt tccagtccgc ttttggtgct gcatggcgcg aacgatccga gagttcctaa
      121 aatacaatca gatcgaatgg tggaagctgt gaaacgaaat ggcggaattg tggaatacct
      181 tgtctttcac gatgaggctc atggcttgcg ccaaccagca aatgtggcaa aggcatacac
      241 tgctatcctc gactttctag atcgatacct ttgacgtgtg ttgcagaaga gtgcgcagct
      301 cactgttacc aagtatgcgc ttcggagccc cttccttagc gagttgaagc atcgcccgcc
      361 ccaactcttc actacttatt attgctgagg gagagacggc ggcaatcaaa ggtagaatag
      421 gacggaccaa cgcatatcca acgcgagtcc aacgcgtctt tgacacttcg ccgtgcattg
      481 gtcgcagagc acctaaacga atcatgtatg aggatggaaa cagtttgagt agttcattct
      541 cagttcgacc ttttacttgc gcccatttcg ccgtaccgcc agtacctgca cccgaaacat
      601 acacaaacgt catccgggga ttaccgcgag ctaacaggtt ggcccaaccc atagtgaggt
      661 caaaggtgag tcgtttgtac tctgcagggt ccatccctac tgaagaaacc ccaagacaga
      721 acaaacacgc atcgaaagct ttcaactccg cttgatctat cgaaaaatca aaaaggtcag
      781 ggagaattat ctcgcgtagc ttttcatggg attgtcgaga tgctcgtcga ccgattgaca
      841 aaactctctc aacacttttg tcgagaaggc attcacgcag aaccgcctgg cccaccattc
      901 cggaagcacc gaatagaatt accttcatga acccatataa ctaattgtga acttgataga
      961 gcgatttcaa agagcgtaac tcaacccgct gaattccacc aacaacttcc tcaaactcac
     1021 tcactgcatg catcattaca aaacgatttc acgctgaagt aagaacgagg tgaagacgcc
     1081 caaaccttca cctcgcggag ttaaacgata cctatccgac aattgctttc agtaactttc
     1141 caagtagtga gctactgttc gatgggggtg caatagtttc cggagtcggg gttggcggcg
     1201 gtggcggcag cggtccgcct ttcggtgatt gcgatgcacc ttgtgggatc ggcgcgggct
     1261 gtaccgggtt cggaattgca aacattaaac cggccaaaag acagacactg attgcaatta
     1321 actttagctt tctcatcttt tctcctttgg tttgaccgca aatgactgcg gttagggttg
     1381 acgttttgtc tgccggttag cagacattgc acggtgaacg tgattcgcta atttcttgag
     1441 gcaagagccg gtggtgagac cgacttctct ttgaactcgc tccattcttt ggaccaacta
     1501 gacttttgtt tcggggtctt ttcgagcagc cagaacttga accaggccag atttcgttcg
     1561 tacacgaaat atttgtgagc cggctgattc tttacgtgcc cttcgtttgg atagatgatc
     1621 agctcaaccg ggacttcgcg ttccttgagt gttgtataga gctgaagtcc acagagatac
     1681 tccgactctg ctgcctgaac caacagcggg gccgtgagat tagtaacatt caatgccggt
     1741 gagagttctc gccacttgtt ctgtcctttt ccttctggac cacttcccat ccaatccgtg
     1801 aagattcggc gccaaccgtc atgggcgagg taataaaaga tcggatcacg agcgctccac
     1861 cctccagttg ctgccgcagc gaacattgaa gagtgcgtaa ttgcaaattc cgtgatctct
     1921 gctccatagc tcaatccagt tagcccaata cgatttggat cgaccagctc ctcacggacg
     1981 agcttccgca caaccacttc cagacttgat agtggcgagt accacgtgag catttgagtc
     2041 ttgaagtctc cgggatcggc cggccgccgg tccggagcgt tgaaggcaag aaccagaaat
     2101 ccgtgtgccg caaatacctg gatcggaaac tcatcaccaa ctgcgcctga taagaaacca
     2161 ccagccgtat aggttgtgat gattagcgga tagcttttgt tggagtcgaa atctaacggt
     2221 ttgaagagat aaccaaacgc ggcatcacca tattcattgg accaatcgag ccgcgacggc
     2281 gcacttagat ttagagtctg gaattctgga tttagatcag tgagggttac tactcgccgg
     2341 tcggaaactc tgatcaacgc gatgttcggt gggctgatcg tgttctcaat aacacaagca
     2401 acagactgct gactggcatc gaaactaaag ttacttagcg gatcaactga acgaattagc
     2461 cttcttatcg gtccgcccga cgcgggaact tcaaagagtc cagctccatt aacagagacc
     2521 tccgcgaagt agatcttatc cccagtgtta ctccagtgga tgtcagaaat gagtgcgttt
     2581 gtagcaggta caaggattat tcgccggccg ctatagtcct ggttgatgag ggaccatccg
     2641 taatatttga aatcctgcgg gctttgaagt cttttaatgt acgccgtaga tttaccgtcg
     2701 ggtgaacgtc gcgcaacata gacgcctgtg tctgttgtgc cgatgttttg atctgcctgt
     2761 tgctgcttaa tctcatcaga gctcagacgg cgttcgtggc cggtagagac cgaatacttc
     2821 caccagtcca tcgctcgcga ctgacgctcg cgtagcaact cggtcaccgg tttcgcacgc
     2881 gaagcacgaa tggtcccatc ataaaccagt ccctgctggg ccagtgtccg aaccgctgtc
     2941 aggtcgatcg gagcaacagt tcggtacaca atctccttac catctttgct ccactcgtag
     3001 gtttgaacgt catgctggct acgagtcaat tgctccggag ggccgccttc tctgggccaa
     3061 cgggtgatct gccgtacgcc gttagacggt aaacagagag ttacatagct actgtctggc
     3121 gaccattgag gcgcaattga aagcagttgt ccaagagaat cgaattccgg cgggccagcg
     3181 ctaccaaatt ccgcgacttt gttcttgtcg ctggtgctgg ctacgaagag acccgttcga
     3241 taactattcg ttgcaacaag cgcctggacc aaaacaaaag cgatactttt accgtcgggt
     3301 gagatcttca cgccgaggac acgtcgcatt tgcagcaaat cgagacttgt aatcgctcgc
     3361 tttggggctt tttcgaattt ccccagtttt acaggtgtgg gatcgaaacc gtgcgcggat
     3421 aactcggact gcccggagca aatggatgcc gaaagaaata cgactccgac gctccatgca
     3481 aatagattag gcagagccgt gcgccatctg tgacccaatg atggaccaac agtttcgacg
     3541 attcggatcg cagtttttct taactttgtc attgccaccc ttaatcagaa ttgtgctttg
     3601 agcgagagtt gaatggatcg aggtccacca atttgataga gaggatttaa accaccagtt
     3661 cctgtgccgc cgctcgcaag actacggccc aacatcgaag tagagcggcc aaaggcggcg
     3721 ttaggaaaga actggccacc gcttgccaac cctaaactca tatcttcgaa cctcgggtca
     3781 ccaaagtttg ggtgattgaa cgcattaaac aactcgcctt tgacctgaag gtcgaagcgt
     3841 tcggccaaac gaaactggcg ccgcagtgcg aagtcgagtt gccaaaacgg atagccgcgg
     3901 actgagtttc tccctaaggt tccctggctg gttggagttt ggaccacgaa tgcagcgggg
     3961 ttaacacgtc ttcctccagc ctcagccttg tcttctatgt ataaaggcgc gtcggaacgc
     4021 cggttaggac gcagttggta aatcccaaat ggtgcgattg ttgtgaagta agcatttact
     4081 ggtgtggctg aacgagcgct gaagatcgag tcgatcgccc aatctttcaa caagccatgc
     4141 aacgctatcg agctcttgac gggaaggttg tacgtgaggg ctgcggatgc agaatggcgc
     4201 acatcaaagt ccgaatcgcc gcggtcctga ctgatattga ctacttcgac cggaacgttc
     4261 gctgaagagt cgtttgaagc aatatcgatg gagtgggacc atgtgtacga gaccagcgcc
     4321 tgcatccctc ttgaaaagcg tcgctgaaat tgaacctgaa gggcctggta gtttgagctt
     4381 gctgaatttc tggtgaagga ggttgttgac gcgaataccg gattattact tgcgccgttg
     4441 aagagcgcgt cttgcctgag cagtctgcgt cccaacgctg cgagataagt cgcggagaga
     4501 gtctgatgcg cgcccaacga cttctcgagt gccaggttcc attggaggct gtaaggcaat
     4561 tttagttcag gatcaaaaat tacgattgtg ctggtcaccg gcaaatttgt ggtaatgggt
     4621 ggcggtgacg cctgagctgg ggaaatcgga aaagtggtat ttgtaaaacg tcgcactgcg
     4681 gcaaatggaa acgatgcgcc ataagcattt gacgtctgcg catttccaag atcatagaaa
     4741 acgcctgctc ctcctctcaa aatcaattca ttaccaggtt cgtccatcaa ctgatagacc
     4801 acgccgaccc ggggagcaaa attgttgtag gttgtctggt aaagtgccgt gcctcgcggc
     4861 gcgagggtca ttgtcggaat attttcaact ccagtgactg caaaggcgaa atttccgttt
     4921 agttcggtcg gtggaggatt gacctcccag cgaaatccat aagtgagtgt tagtcgtcga
     4981 tgagctctcc aggcgtcctg ggcaaagagt gagagattgg tatacctcaa ctcctgtggt
     5041 ccagtttgcg catctacctg gaccgagctc gctagcgacg cgatcgcgtt agttacattt
     5101 ccaaactgaa tcgtctgcgc gtatccacga atgccgatga cggggaggag caatcggtag
     5161 tcaacgccga acttgagttg atgacttcct attacagcgg acaagctatc gacgatattg
     5221 aattggcgtt gccggttaga aacgttggtc cctgcctgga tcgatgcatt tcggccacct
     5281 gtaattgaaa gcctgaaaaa ggcatcagaa aacgagacga atgagggaaa aagaagtgac
     5341 tctgccggga cagcagcgcc accaaacgta tcgaggcgac tccagctctt accaaagaca
     5401 ttgctgaaat tgaatctcaa ttcattaaac gtcgttgctg aaaagctgtt cgttgcgcca
     5461 acggttaagg tttgagtgcg gtttgagaag acgcgaatac tattgagact gcgtaagcca
     5521 tctcctgccc ggctcagaga ctccgatggg gcatagttat atcttccaaa cagtgtgaaa
     5581 cgagcgttga atgaatgatc
//
LOCUS       DASSFP010000463         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_886882, whole genome shotgun sequence.
ACCESSION   DASSFP010000463 DASSFP010000000
VERSION     DASSFP010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886882"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..636)
                     /locus_tag="VFH91_10420"
     CDS             complement(70..636)
                     /locus_tag="VFH91_10420"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET6863457.1"
                     /translation="MTAATAVAAEKAMVGQPAPDFEMASTKNIEKLDNPVKLSDYQGK
                     WLVLVFYPLDFTFVCPTELTTFSDRYQDFQDIGADIIGISTDSVFSHRAWLNTSRDKG
                     GVEGLKYPLAADATKKVAADYGVLIENRGIALRGLFVIDPEGILRYKVVHDLNVGRSA
                     EETLRVIQALQTGGLCQAEWKPGQKTLS"
     gene            complement(682..1098)
                     /locus_tag="VFH91_10425"
     CDS             complement(682..1098)
                     /locus_tag="VFH91_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET6863458.1"
                     /translation="MKAGKFNLAERAGQKLTPQREAVFQVILDREDHPTASDIFEAAR
                     QRLPTISYATVYNSLRYLKEAGLVHEISFGDSASRYDRETDRHDHAICNECGKLVDFE
                     LPEAAQLMQAAARKSRFKAQSVHLTLRGICPDCKDA"
     gene            complement(1150..2232)
                     /locus_tag="VFH91_10430"
     CDS             complement(1150..2232)
                     /locus_tag="VFH91_10430"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6863459.1"
                     /translation="MTSTETFFITGFPGFIATRLLHRLAASESKFLLLVQPSLMDLAR
                     SEIQQIASETKRDTADFEVLPGDITRNGLRLNDADLEKVRARTTRLFHLAAVYDLAVN
                     RDLAQLVNVLGTKNVNEFARALPGLRQYHYVSTCYVAGKREGRILETELQHDKGFRNF
                     YEESKYQAEMEVEQLKSELPITIHRPAVVVGDSQTGQIPKYDGVYYLIFYLLRWPGLL
                     SQINIGNHQVSLNLVPVDFVVEAMSALSLDDSAIGRTLQLADPQPLSTNELFDTIAGS
                     ISGRTSRVTAPARLVQFFLMLPPSPRITGLPHHGVPYFFVKQTYDTSIARRLLLPHGI
                     VCPSFGNYVRQIVEYAEHHPEHRLIV"
     gene            2437..2931
                     /locus_tag="VFH91_10435"
     CDS             2437..2931
                     /locus_tag="VFH91_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6863460.1"
                     /translation="MNDDNPSAERFKAKQLFHEAYEAQLAHDYERAIELYKRSIETHP
                     TAEAHTFLGWVYSFEERYDEAIAECLEAIRVDETLGNPYNDIGSYLLAKGDTYGSVRW
                     FRRALLAPRYESYAFPHFNLGRVYESRRRFLEATKHYGLALKEKPDFTEAAVSLRRMQ
                     AKTN"
     gene            complement(2960..3343)
                     /locus_tag="VFH91_10440"
     CDS             complement(2960..3343)
                     /locus_tag="VFH91_10440"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HET6863461.1"
                     /translation="MSENRDPAIRITLLPRDTNSQGTIFGGVILSYIDTAGAVEAHRH
                     TRMARFVTVAMREVIFHKPVFVGDLVSFYCETVRVGASSITVRVIVEAERLGTDGRIR
                     VTEAEVVYVAVDREGNKLHIKRRGK"
BASE COUNT          929 a          916 c          846 g          882 t
ORIGIN      
        1 attcctaaga aaggttgttc ctgtacacga gggtgccggt ttaacgcgac accctcgctc
       61 caaaagttat taactaagtg ttttctgacc tggcttccat tcagcctgac agaggccgcc
      121 ggtttgaagc gcctgaataa cacgtagagt ctcctcagcc gagcgaccga cattaagatc
      181 gtgaacgacc ttgtaacgaa ggatgccttc aggatcgatc acgaatagtc ctcgcagagc
      241 gataccgcga ttctcgatga gcactccata atcggctgcc actttctttg tggcatccgc
      301 ggcaagtgga tacttcaaac cttctactcc gcctttgtcg cgagaagtgt tcaaccaggc
      361 ccggtgcgaa aacacggagt cggtcgagat tccgataatg tcggcgccga tatcctggaa
      421 atcctgatag cgatcgctaa aagtcgtcaa ctcagttggg caaacaaaag tgaaatcaag
      481 tgggtagaaa accagtacga gccactttcc ctggtagtcc gatagcttta ctggattgtc
      541 gagcttttca atgtttttcg tcgaggccat ttcaaaatcc ggcgcgggct ggcccaccat
      601 cgccttttct gctgctactg ctgttgctgc tgtcatgtat cctccaaaat agttgaaata
      661 ttccttattg ggccgcttct attaagcgtc tttgcagtcg ggacagatac cacgcaatgt
      721 gagatgtacc gactgagctt tgaatcgaga tttacgtgct gccgcttgca tcagctgggc
      781 agcttcggga agttcaaaat cgacgagctt cccgcattca ttacagatgg catgatcgtg
      841 tcggtcagtt tcgcggtcgt agcgactggc actatcgcca aagctaattt cgtgaaccag
      901 accagcttcc ttgagatagc gcagagagtt atagacggtg gcataactaa tagttggaag
      961 ccgctgacgg gccgcttcaa agatatcgct tgcggttggg tggtcttctc ggtcgagtat
     1021 gacctgaaaa accgcttcgc gctgcggcgt cagcttctgc cccgcccttt ctgccaggtt
     1081 gaactttcct gccttcatga tggatttaga ctaattctaa gctgaaggaa agtcaagttg
     1141 ggaggcaaac taaacaataa gtctgtgctc tggatgatgc tctgcgtatt caacgatctg
     1201 cctgacgtaa ttcccaaacg atggacaaac aatcccgtgc ggcaaaagta gtcgtcgcgc
     1261 gatgctcgtg tcgtacgtct gcttaacgaa gaaataggga acgccgtgat gaggcaaacc
     1321 tgtgatgcgc ggcgaaggag gaagcattag aaagaattgg accaatcttg caggagccgt
     1381 tactcgcgat gttctgccac tgattgatcc tgcgatcgtg tcaaaaagct cattggtcga
     1441 aagcggttgc ggatcggcta gctgaagcgt cctaccaatc gccgagtcat caagcgacag
     1501 tgcggacatt gcctcgacta caaaatcaac cggtacaaga ttcaagctga cctgatggtt
     1561 cccgatattg atctgcgaaa gtagcccggg ccagcggagc aagtaaaaga tcagataata
     1621 aacaccatca tatttcggaa tctgacccgt ttgtgaatca ccgactacaa ctgccggacg
     1681 atgaatggtg atgggcaact cactcttaag ctgttccact tccatctcag cctgatactt
     1741 cgactcttca tagaaattcc tgaagccttt gtcgtgttga agctcagtct cgaggatcct
     1801 tccttccctt tttccggcaa cgtagcacgt tgaaacatag tgatactgcc gcaaccctgg
     1861 caaagccctg gcaaactcat tcacattttt cgttccgagg acatttacca actgagcgag
     1921 atctctgtta accgcaaggt cgtaaacggc tgcgaggtga aacagtcgcg tggttcttgc
     1981 acgtaccttt tctaaatccg cgtcattcag tctgagccca ttacgggtga tgtcacctgg
     2041 caacacctca aaatccgcgg tgtcccgttt tgtttccgag gcgatctgct gtatttctga
     2101 ccgagctaaa tccatgagtg acggttggac caaaagcaaa aacttgctct ctgaggcagc
     2161 cagccgatgt agaagccgcg tcgcaatgaa accgggaaag ccagtaataa aaaatgtttc
     2221 agtcgaggtc attttcgtat ctttcggtcc aagcccacgt acatcactgc aaaacctatt
     2281 cgagtgtttc gccaacgagt ataattcaag gcctccgtta actcattcca aaggttattg
     2341 ataagcgtgc aaaaggtggt gaggccgcga gtcgaaggtc gaattaccag attggataaa
     2401 acgacaaacc agcaaactga ttgccagtag aaaatcgtga acgacgataa tccatcagca
     2461 gaacgcttca aagcaaagca actctttcat gaagcgtatg aggcccagct ggctcacgat
     2521 tacgagcgag ccattgagct ttacaagcgc tcgatcgaga cacatccgac tgctgaagcc
     2581 catacttttc tcggctgggt ctatagcttc gaggagcgtt acgatgaagc tatcgctgaa
     2641 tgtttagagg ctattcgggt tgatgaaacg ttgggcaatc cttacaacga catcggcagt
     2701 tacttgctgg caaaaggtga cacgtatggg tcagtgcgtt ggttcaggcg agcattactt
     2761 gctccgcgtt acgagtctta cgcatttccg catttcaatc tcggtcgagt ttacgagagc
     2821 cgtcgaagat ttctcgaagc cactaagcac tacggtttgg ctttgaagga aaagcctgac
     2881 tttactgaag cagcagtctc acttcgcagg atgcaagcta agacaaactg aaagaccaaa
     2941 gctcaaaggc caagggccat catttacctc gtcgcttgat atgtaactta ttcccctctc
     3001 tatcgactgc tacgtaaaca acttcggctt cggtcacgcg aatgcgtcca tcggtgccaa
     3061 gtctttcagc ttcaacaatg actcgcacag tgattgatga tgcaccaact cgaactgtct
     3121 cgcagtaaaa acttaccaaa tcaccaacaa agaccggctt atgaaaaatt acttcgcgca
     3181 tcgctaccgt cacaaaacgg gccatccttg tatggcggtg tgcttcaacc gctccagcag
     3241 tatcgatgta acttaagatc accccaccga aaatcgtacc ttgagagttc gtatcccgcg
     3301 gcaacaacgt tatgcgaatt gccggatccc gattttcact cataattcgg atgactcctt
     3361 cattctcaag ataacctgtt tacaggtcgc cagtcgctga acgatcgact cctggaacaa
     3421 acgaaccagc agcgctgtct aataaacatc tgtcgcccat tgcggcggat accagtttag
     3481 aaaggtggac catatcgatg ctcttaacaa tcagcagcgt cctcttgctc gtcctctacc
     3541 tacaagacga ggcaaccaac ttcaacgatt tgg
//
LOCUS       DASSFP010000464         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_291337, whole genome shotgun sequence.
ACCESSION   DASSFP010000464 DASSFP010000000
VERSION     DASSFP010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291337"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VFH91_10445"
     CDS             <1..1387
                     /locus_tag="VFH91_10445"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6863462.1"
                     /translation="AINLLEKATRKQPNNPDPYFHLGQAYQGAGKHQQAIEVLQKSIS
                     LTPSLDHNDYQVTTAHYRLGQSLIKVGRTDEGEKELQTSAELKAKGFKNDEKRVGAFL
                     SGQTLPSNDGKTAELVKAEGVIAEASDLDEKTLDKLKTDESYYTKVIAAAYNSIGLLR
                     AERQDFREATTHFALAAKWNPQQEGLNYNLGLAYYKSESYKDAVPPFEAALKVDPSNI
                     AIKQLLGLSYFMIAEYPKASALLTEVVAVKTQDAALYYPLALSLIKDGKIEAANRVIE
                     QMVAVGDKSPQLHILLSQAQYERGETAKALEELRIALSLDDKLRLAHFYTGLIHLKEG
                     KLQDAAREFDKELALNPSDIQAKYHLAYVILAQQETDRGIRLMREVVQERPDFADARY
                     ELGKALLQNGDIKGSVDSLEIAARLKPDQAHIHYQLGRAYLAAGRKKEGESQLEISRE
                     LKDKARSKTDQ"
     gene            1410..3113
                     /locus_tag="VFH91_10450"
     CDS             1410..3113
                     /locus_tag="VFH91_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HET6863463.1"
                     /translation="MPLRRYALVALPLLALLFRAPVSVTQPAKIQFTDITSSAGITFK
                     HVASPDKRYIVESMSGGVAMFDYDNDGDLDIYLVNSLTVDLVKSKTKTKSALYRNDGD
                     GKFTDVSEKAGVSDIGWGMGVAVGDYNNDGFEDLYVTCLGPNHLLKNNGNGTFTDVTE
                     KAGVGDPRWSTGASFFDYDRDGYLDLFVANYVDFDLNNLPEFGQGKSCQYKSIPVQCG
                     PRGLKGAGDTLYHNNGDGTFTDVSKQAGVSDPNGFYGLGVITSDFDDDGLIDIFVAND
                     STPNFHYRNQGNGTFKEIGFLAGDAVNENGSEQGSMGVTVGDYDHDGKLDLFVTNFDD
                     EYNVLYHNNGHNSFTDVSYAAKVAAVSLPYVGWGTKFFDYDNDGWVDIFVANGHVYPQ
                     LPSYRQRRLLHRNNHDGTFTEVSAQFGAMLTENRASRGVAFGDIDNDGDVDLLIADLD
                     GPPQLLRNDGGNTNNSILIKTVGVKSNRSGIGARVKVVAGDLIQLDEVRSGDSYISQS
                     DLRLHFGLEKRTKVDLIEVRWPSGLVDRITGVGVNKIVTIKEGQGKIEEKEYARSISQ
                     R"
     gene            3149..3763
                     /locus_tag="VFH91_10455"
     CDS             3149..3763
                     /locus_tag="VFH91_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6863464.1"
                     /translation="MIKTAVTLLLVVVFLGQQGTLRRTVAFTKTLQQVESNRFSQGAG
                     VVAQKQDAVEQFEHLASLIREDRIKEAEAQLTRILNAKPNHPVALNLMGTIRAKQGRV
                     NEAEGLFLRAIRSDNQFAGAHMNLAYIYLLQRAPEKAIGQFKDVIGLEPDNSEATEKL
                     ADLLLSQKQLDECIRLIEQQRKSRSISPTLLVTLGDAYLAKGAQ"
BASE COUNT         1053 a          923 c          999 g          878 t
ORIGIN      
        1 agctatcaat cttcttgaga aggcaactcg caaacaaccc aacaatcccg atccatattt
       61 tcacctgggc caggcgtacc agggagcagg taaacatcaa caagccatcg aggtacttca
      121 gaagagtatt tcgctgacac cttcgctgga ccataacgat tatcaggtta cgactgcgca
      181 ctaccggctc ggtcaatcgt tgatcaaagt tggtcgcacc gacgagggtg agaaggaatt
      241 gcagacttca gccgaactca aggccaaagg atttaagaat gatgagaaga gggttggggc
      301 gtttctcagc ggacaaactc tgccgagcaa tgatggaaag actgccgaac ttgtcaaagc
      361 agaaggtgta attgctgaag cgtccgacct tgatgagaag actctggaca agttaaaaac
      421 agacgagagt tactacacaa aagttattgc ggcagcttac aacagcatcg gcctgcttcg
      481 ggcagaacga caagactttc gcgaggccac aacacatttc gccctcgccg ctaaatggaa
      541 tccacagcag gaaggactca attacaatct tggtctcgct tactacaaat ctgaatctta
      601 taaagatgct gtgcccccat tcgaagcagc gctaaaagtt gatccctcta acatcgcaat
      661 caaacagttg ttaggcttga gctatttcat gatcgcggag taccctaaag cttccgcgct
      721 gttgactgaa gttgttgccg taaagaccca ggatgctgcg ctctattacc cgcttgcact
      781 ctcgctcatt aaagatggca agatcgaggc ggccaacaga gttattgagc agatggttgc
      841 cgtgggcgac aaaagtccgc agcttcacat cttactgagc caggcacaat atgaacgtgg
      901 tgaaaccgcg aaagcacttg aggagctacg aatcgcactt tccctcgatg acaagctgcg
      961 gctcgcacac ttttatactg gactgatcca ccttaaggag ggcaagcttc aagacgccgc
     1021 acgcgagttc gacaaagagt tggcgctaaa cccgagtgac attcaggcca aatatcatct
     1081 ggcttacgtt attctggccc aacaggaaac tgatcgcggt atacgtttga tgcgagaagt
     1141 ggttcaggag cgtccagatt tcgccgacgc tcgttacgaa cttgggaaag ccctattgca
     1201 gaacggcgat atcaaaggga gtgtcgacag tcttgaaata gccgcacggc tcaaacccga
     1261 tcaagctcat attcattacc agcttggccg tgcctatctt gccgccggtc gcaaaaaaga
     1321 gggagagagc cagctcgaga tctctcgtga attaaaagat aaggcgcgct ccaaaacaga
     1381 ccaatagact cgagagagga cagccacgtt tgccactaag acgatatgcc ctcgttgctt
     1441 taccactcct ggcactctta tttcgcgccc cggtaagcgt cacgcaacct gcaaagattc
     1501 agttcaccga catcactagc tccgctggaa tcacgttcaa acatgtcgct tcccctgaca
     1561 agagatatat agtcgaatcc atgagcggcg gcgtggcgat gttcgactac gacaatgatg
     1621 gcgatctcga catctatctt gtcaattctc taacagtgga tttggtgaaa tcaaaaacga
     1681 aaacaaagag tgcgctctac aggaatgacg gggatggaaa attcacagat gtatctgaaa
     1741 aagcaggcgt aagcgatatt ggatggggca tgggagtagc cgttggcgac tacaacaacg
     1801 atggcttcga ggatctatat gtcacctgcc tcgggccaaa tcatctctta aaaaataatg
     1861 gcaatggcac atttactgat gttaccgaaa aagcgggagt aggcgatccg cgttggtcta
     1921 ctggagcgtc gttctttgat tacgatcgtg atggctacct ggatctgttt gtcgcgaatt
     1981 atgtcgattt cgatttgaat aacctgcccg aattcggtca ggggaagagt tgccagtaca
     2041 agtcaatacc tgtgcaatgt ggcccaagag gattaaaagg agcgggcgac acgctctacc
     2101 ataataacgg cgacggtacg tttacagatg tcagcaaaca ggccggcgtg tctgacccca
     2161 acggcttcta cgggctggga gtcatcacaa gcgacttcga cgatgacggt ctaattgata
     2221 tcttcgtcgc caacgattcg acgccaaatt tccattacag aaatcagggg aatgggacct
     2281 tcaaagagat cgggtttctt gctggagacg cagtaaacga gaatggtagt gagcaaggca
     2341 gtatgggtgt gaccgtgggt gattacgatc atgatggaaa acttgatcta ttcgtcacga
     2401 acttcgacga cgagtacaac gtcctctacc ataataacgg gcataattcc tttaccgatg
     2461 tctcatatgc tgccaaagtt gcagcagtga gtttgccgta cgtaggctgg ggaaccaagt
     2521 ttttcgatta cgacaacgac gggtgggtcg acatatttgt ggccaacggt cacgtttatc
     2581 cacaactccc gagttatcgc cagcggcggt tattacatag aaataatcac gacggaacct
     2641 tcaccgaagt atccgcacag tttggtgcca tgctgactga gaatcgggca agccgcggcg
     2701 tggcatttgg cgacatagac aatgatggcg acgtcgattt gttgattgct gacctggatg
     2761 gtccacccca actgctgaga aacgacggtg gtaacaccaa taattcaatc cttatcaaga
     2821 ccgtcggtgt aaagagtaat cgtagtggca taggcgcgcg cgtgaaggtt gttgctggag
     2881 acttgattca actcgatgag gtccggagcg gtgatagcta tatctcgcag agtgatctcc
     2941 gacttcactt cggtcttgaa aagagaacaa aagttgacct gatagaagtg cggtggccga
     3001 gcggactcgt tgacaggatc acgggcgttg gagtcaataa gattgtgact atcaaggaag
     3061 gtcaaggcaa aatcgaagag aaggaatacg ccagaagcat ttcgcaacgc tagactcgcg
     3121 tggctttgcg tctaagatat taatccggat gatcaaaaca gctgtcacac ttcttctagt
     3181 tgtcgtgttc ctgggccagc agggaacatt gcgccgaact gttgccttca ccaaaactct
     3241 tcagcaagtc gaaagtaatc gcttcagtca aggggctggc gtcgttgccc agaaacaaga
     3301 cgcggtcgaa cagtttgaac atttggcttc gcttattcgt gaggatcgaa ttaaggaggc
     3361 tgaggcgcag ctcacaagaa ttcttaacgc aaagcccaat catccggtgg cgttgaacct
     3421 catgggcacg attcgcgcca agcagggtcg cgtaaatgag gctgaagggc ttttcctccg
     3481 tgccatccgc agtgacaatc agttcgcggg ggcgcacatg aacctggcgt atatctatct
     3541 gctgcagcgc gctcctgaga aagcaattgg ccagttcaag gacgttattg gtctcgaacc
     3601 agacaattca gaggcaactg aaaagctcgc tgacttattg ctgtctcaaa agcaacttga
     3661 tgagtgtata aggttgatcg agcagcagcg gaagtctcgg tccatctctc caactttgct
     3721 cgtcacactg ggtgatgcct acctggcaaa aggcgctcag taggaagctg aagaggctta
     3781 cctgtcggcg cttgaagggc ggcttgaaaa tggaggtgct ctatttggtc tggcccaact
     3841 ctcctattca aaa
//
LOCUS       DASSFP010000465         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_888177, whole genome shotgun sequence.
ACCESSION   DASSFP010000465 DASSFP010000000
VERSION     DASSFP010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888177"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VFH91_10460"
     CDS             complement(<1..1346)
                     /locus_tag="VFH91_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET6863465.1"
                     /translation="MEEYSDRTPADVAWYPMVPIRDVVIFPFTKVAFKIGRPGSVRAL
                     EQALATDRTIFLATQHDATVDEPTPEQIYGVGTLGRILQSQKQDNGQIKVVVEGRERA
                     TTVRVENSDGAFMALVRRAPVVNEEGTRLDALIQKVGQLVEQHLRLAPDTQTDALQTA
                     LRNQEPSHLADALASQLRISVEDKQGLLEVFSTQARLQRLIELLETEIEKRQLDRTIQ
                     TRVKRQMEKAQKEYYLNEQIKAIHKELGRKDEKAELEELKKKIEEAGMTDEAKEKAMQ
                     ELHRLEAMPPMSAEGTVSRTYIDWLISVPWKQKSKEIKDLIKAEEVLNEDHFGLEKIK
                     ERILEYLAVRQLVKNPRGSILCFVGPPGVGKTSLGKSIARATGRKFIRLSLGGVRDEA
                     EIRGHRRTYIGALPGQIIQMMKKAGTVNPVLLLDEVDKLGADFRGDPSAALLEVLDP"
     gene            complement(1492..2736)
                     /gene="clpX"
                     /locus_tag="VFH91_10465"
     CDS             complement(1492..2736)
                     /gene="clpX"
                     /locus_tag="VFH91_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET6863466.1"
                     /translation="MVRRTDDTLRCSFCGKSQNEVKKLIAGPTVYICNECIDICNEII
                     TDDQQAESVATRPPLPKPGEIKNFLDEYVIGQEETKKRLAVAVYQHYKRIELGRRRSD
                     VEIQKANILLIGPTGTGKTLLAQTLARMLAVPFTIVDATTLTEAGYVGEDVENIILKL
                     LQAANGDVERAQQGIIYIDEVDKICRKDENPSITRDVSGEGVQQALLKILEGTVANVP
                     PQGGRKHPHQEFFPVDTTNILFLCGGAFVGLEKVIERRLRNKSLGFHAEVKSGTHRHA
                     EALAAVQPEDLIRFGLIPEFVGRLPVAGVLHELDEGALIKILTEPKNALVKQYQKKFE
                     FDNVKLKFTEDALKAVARQAVERKVGARGLMMILEELLLDAMYTLPSQKRVKEFVITS
                     EMVERRRAVPGEGLTGIDGAAA"
     gene            complement(2770..3354)
                     /gene="clpP"
                     /locus_tag="VFH91_10470"
     CDS             complement(2770..3354)
                     /gene="clpP"
                     /locus_tag="VFH91_10470"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET6863467.1"
                     /translation="MALVPMVVEQTSRGERAFDIYSRLLKDNIIFLGTPIDDQIANLI
                     VAQLLFLEAEDPEKDINLYINSPGGSITAGMAIYDTMQFIRPDVTTICVGQCASMGAL
                     LLAAGAKGKRFALPNSRILIHQPSGGSQGQATDIRIQAEEILRMRELTSQIIAKHTGQ
                     SYDTVERDVERDKIMSAMQAKEYGLIDEVIAHRE"
BASE COUNT          771 a          928 c          925 g          991 t
ORIGIN      
        1 ggatcgagaa cttctaataa cgcggcactc gggtcgccac ggaaatccgc ccccaactta
       61 tccacttcat ccagtagcag aacgggatta acagttcccg cctttttcat catctgaatg
      121 atctggccag gaagagcgcc gatataagta cgacggtgac cgcgtatttc tgcctcgtcg
      181 cgtactccgc caagtgaaag tcgaatgaac tttcgtccgg tggctcgagc gatcgattta
      241 ccaagactgg tttttcctac gcccggaggt ccgacgaagc agaggatcga gccacgtgga
      301 ttcttaacga gttgtcgaac ggcgagatat tcaaggatgc gttccttgat tttctcgagt
      361 ccgaagtgat cctcgttaag aacttcttcg gctttgatca ggtccttaat ttctttggac
      421 ttctgcttcc acggcacact gatcagccaa tcgatgtagg tgcgtgaaac agttccttct
      481 gccgacatcg gtggcatcgc ttccaggcgg tggagctcct gcatggcctt ttctttggcc
      541 tcgtccgtca tgccggcttc ttcgatcttc ttcttgagct cttccaactc agctttctca
      601 tctttacgtc cgagctcttt atggatggct ttgatctgct cgttgaggta gtactctttc
      661 tgagcttttt ccatctggcg tttaacacgc gtttgaattg tgcggtcgag ctggcgtttc
      721 tcgatctcgg tttccaacaa ctcgattagc cgttgcagtc tcgcctgagt cgaaaagact
      781 tcgagtagcc cttgtttgtc ttcgactgag attcgcagct gagatgcaag cgcatctgca
      841 aggtgcgagg gttcctgatt tctcaaggca gtctgcaaag cgtcggtttg tgtgtcggga
      901 gccagtcgca gatgttgttc taccaactgt ccgactttct ggatcaacgc atcaagtctg
      961 gttccttctt cgtttacgac tggagcgcgg cgcaccaatg ccatgaaggc gccatcgcta
     1021 ttttcaacgc gaaccgttgt ggcgcgttcc cgaccttcga ccacaacttt aatctgaccg
     1081 ttatcttgtt tctgagattg tagtatccgg ccgagagttc cgactccgta gatttgttca
     1141 ggagttggtt catcgaccgt ggcatcgtgc tgcgtcgcca aaaagatcgt ccggtcagtg
     1201 gccagcgctt gttccaaagc gcgaactgag ccgggacgtc ctatcttgaa agcgactttt
     1261 gtgaaaggaa atatgacgac gtcacggatt ggtaccatcg gataccaggc aacgtcggcg
     1321 ggggttcgat ctgaatattc ttccatgatt taagtgacgt tattctgagc cagtgcaaca
     1381 aaggcgagcc gctaaaactg ccgtctcgcc tctcaataag agatgcctaa tagtagatca
     1441 tttcccgtgg gtgcgctact ttcccgtttg gacgcgctgg ggacgaaaag gttaggctgc
     1501 cgcgccgtcg attccagtca aaccttcacc gggaaccgca cgtcgtcttt cgaccatttc
     1561 gctggtgatt acgaactctt ttacgcgttt ctgcgagggc agggtataca tcgcgtcaag
     1621 caggagttcc tcgaggatca tcatcaggcc gcgggcgcca actttcctct caacggcttg
     1681 tcgggcaacg gccttgagag catcttcggt gaacttcagc ttaacgttat cgaactcgaa
     1741 tttcttctgg tactgcttga cgagggcgtt tttcggttca gtaaggattt ttataagagc
     1801 gccttcatcg agttcgtgca ggacgccagc gacgggcaat ctaccaacga actcaggaat
     1861 taagccaaaa cggataaggt cttcaggttg tacagccgcc agcgcttcgg catgacggtg
     1921 cgttcccgat ttaacttcag catggaaacc gagcgacttg tttcggagtc gtcgttcaat
     1981 aaccttttcg agtcctacaa aggcgccacc gcagagaaac agtatgttgg tggtatcgac
     2041 cgggaaaaac tcctgatgtg gatgcttgcg cccgccttga ggcggaacgt tagccaccgt
     2101 accttcaaga atctttagaa gtgcctgttg aacaccttca ccggagacgt ctcgtgttat
     2161 cgacgggttc tcgtccttgc gacagatctt gtcgacctcg tctatataga tgattccttg
     2221 ttgagcacgt tcgacatcac catttgctgc ttgcaggagt ttgagaatga tgttttcaac
     2281 atcctcgcca acataacccg cttcagtaag tgtggtggca tctacgatcg taaagggaac
     2341 tgcaagcata cgcgcgagtg tttgcgcgag tagtgttttg cctgtaccgg taggtccgat
     2401 aaggagaatg ttggctttct ggatctcgac gtcactgcgg cggcggccga gctcgatccg
     2461 tttgtaatgc tgatatacgg caacggccag gcgcttcttg gtttcttcct ggccgattac
     2521 gtattcgtct aagaagtttt tgatctctcc cggtttcggc agaggcggtc tggtcgctac
     2581 ggactctgcc tgttgatcat cggtgatgat ctcgttgcag atgtcgatgc attcgttgca
     2641 gatgtaaacg gttggaccgg caatgagctt cttaacttca ttctgtgact tcccacagaa
     2701 cgagcagcgc agagtgtcgt cggttcttcg taccatatgc taaagaggaa gctccttggg
     2761 actcttcggt tactcgcgat gcgcgatgac ctcgtctatt aaaccatatt ccttggcctg
     2821 catggcagac attattttat cgcgttcaac atcacgttcc actgtgtcgt aggactgccc
     2881 cgtgtgtttg gcgattattt gagacgtcaa ttcgcgcata cgaagaattt cttcagcttg
     2941 aattcttata tccgtcgctt ggccctgcga tcctcccgac ggttggtgaa tgagaattcg
     3001 cgaattcgga agtgcaaatc gcttgccctt tgcgcccgcc gctagaagca acgcacccat
     3061 cgatgcacac tggccgacac agattgtagt cacatctgga cggatgaatt gcatcgtgtc
     3121 gtagattgcc attccggcag tgatcgatcc gccgggtgaa ttgatgtaga ggttgatgtc
     3181 cttttcggga tcctcagcct cgagaaagag cagctgggcg acaatcagat ttgcaatttg
     3241 atcgtcaatc ggagtaccga gaaaaataat gttgtctttc aacaaccgcg aatagatatc
     3301 gaaagcacgt tcgcctcgcg acgtttgttc aacaaccatt ggaactagag ccataacaga
     3361 aacctcgctt agagcttatc ggggattgga gttcagtgtt acgaagatgt cttttaagaa
     3421 tgagacttca tgaagtgagc cggggataga gcggggctac tcatcctgtg tactagcgcc
     3481 ttcgacgttc tcttcttcgc tccactcttc ttccgttacg tgcgcattct cgatcaaaaa
     3541 atcaagcgct ttacgattgc gcaatctgtg cgcgatgcta cgttcgcctc catcctttgt
     3601 caaggcagcc cgcac
//
LOCUS       DASSFP010000466         6152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1919829, whole genome shotgun sequence.
ACCESSION   DASSFP010000466 DASSFP010000000
VERSION     DASSFP010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919829"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="rpoN"
                     /locus_tag="VFH91_10475"
     CDS             <1..1248
                     /gene="rpoN"
                     /locus_tag="VFH91_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET6863468.1"
                     /translation="SFEEGGGEEPVGEEAPRDAFEEIDFGREFQDYLDPGYKTQEIEY
                     KEDAPTFEQFLTRPPSLAEHLEWQLHMSPIKSDVCDAAICVIGNLNADGRLNATNEEM
                     AAMEKCSEEVVEEARQAIMRLDPVGCGARDVRECLLVQLEVLGQKDRLAATLISEHLS
                     DLQQHKLPHLSKQIGIDVEVLLEELQFIRTLDPYPGRRYSSDEPILISPEIYIEKLDE
                     NDDEYIIYFADDGSPRLRVSQQYQQMLSQGVSNETKSFIREKMRSAVDLLRNIEHRRQ
                     TIYKVVESIVQRQHEFLDHGVQSLKPMMLKDIAEDIGMHLSTVSRVVNRKYAHTPQGV
                     IELRRFFTEGMLNEDGEEISTRIIKLQIKKLIEEEDSHNPITDDQVVKILAKDGIKLS
                     RRTVAKYRDQMQIPGSRERRAVV"
     gene            1370..1903
                     /gene="raiA"
                     /locus_tag="VFH91_10480"
     CDS             1370..1903
                     /gene="raiA"
                     /locus_tag="VFH91_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6863469.1"
                     /translation="MKFEYTGRHVDVSPAIRRHVEGHFKKLDHIFTDSTLSTHIIIDV
                     EKNRQIGEIIVYWREHTLTAKDTNADMYMALTRAIAKIEKQALKLKKKIIDRKQGGQS
                     TGRVAPDPDGHLEATPRPPRIIAARRYTVKPMTAEEAALRLSGEPDQFLVFRDADTSR
                     LGVLYKRKDGNYGLIEP"
     gene            2023..2952
                     /gene="rapZ"
                     /locus_tag="VFH91_10485"
     CDS             2023..2952
                     /gene="rapZ"
                     /locus_tag="VFH91_10485"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET6863470.1"
                     /translation="MARNPTIQQPPASSNHVPDIVIITGLSGSGMSSATNAFQDLGYF
                     CVDNLPLTMLQTFARLITSEDDEKVIRRAALVIDIREGVFLAEFEKQLLALRGSGLTV
                     TVLFFEASDDVLQHRFSETRRPHPAERGHGLLEAISSEREAMASIRALADQIIDTSDH
                     TVHSLRRFLLDRFSPELAGAPMRVQILSFGHKYGNPGDLELLFDVRHLPNPHFVPELK
                     PLSGHDSRVVKYLRSNQEVQETISRFTDLLTYLLPLYKREGKSYVTVGIGCTGGRHRS
                     VMVANALARALRRAGFEVHARHRDVRKGASRGR"
     gene            3087..3551
                     /locus_tag="VFH91_10490"
     CDS             3087..3551
                     /locus_tag="VFH91_10490"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET6863471.1"
                     /translation="MSNTLQNTPQPSDVKRTSGVIVTHGHLAGELLAAAEMIIGPISH
                     IAAVSIGWHDDVDAARDEVQRAINRVSQGRGVLLLTDMFGGTPTNIASMFLEENAVEV
                     VTGVNLPMVIKLATSPAEESLAEIARKICDQGRQGIYLAGALLAPPSKTGAD"
     gene            3553..5625
                     /gene="ptsP"
                     /locus_tag="VFH91_10495"
     CDS             3553..5625
                     /gene="ptsP"
                     /locus_tag="VFH91_10495"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET6863472.1"
                     /translation="MTERKLRVTGRLGLHARAAATLVRIATQFRSEIKLQRVDRGGDQ
                     AEAADAKSILSILSLAASCGTELLLTAEGVDEEQAVNEITVLFIRQFDEGEPIEVERH
                     ASTIPEFRVKGLGVSDGVAVGRVLRVQGATSDLYRASIDEQDIERERRRFRAAVRASR
                     TQLESIKKRAERELGRGHAYIFDAHLLFLDDAKLTRDVEDYIVKEHANAEWAARVVGD
                     RLLSIYTQISDEYLRERGSDIEDVIQRLLANLTGEERTYPSLSEDAVIVAQDILPSTV
                     AELDLKYVKAIATDAGGWTSHMSIIARGLGLTAVVGLRDFYHRARTGEKIIVDARRGE
                     VILHPLPATIDQYQFSTESIPAPSPVKEEAIFGPVKTRDGVSITLRANVELPTEFEAV
                     RRFGASGIGLFRSEFLLSRPGLMLSEEDQYLAYRRLIEAAGEHGAVVRLFDVGGEIGR
                     DLSERLERNPALGLRAVRFDLRHPEVMRTQVRAILRAATAGPLSLVLPMVADVADVRQ
                     AKSVIEHELSDMRATGIAVSPVRVGAMIEVPSAVLTSEKIAREVDFFELGTNDLVQYT
                     LAVDRGNHEVSDWFRTLHPAVLYEIDRTLASARQAGIPVIACGEMASTPAYAVLLIGM
                     GSVDLSMTPALIPRVRSILSQIDASAAEEIARRCLGCATADEVEEIVRVEFRRRWPDL
                     FPPKSLPA"
     gene            5626..6093
                     /locus_tag="VFH91_10500"
     CDS             5626..6093
                     /locus_tag="VFH91_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863473.1"
                     /translation="MKTSPARLIHVLIGKAVLETIFVAALALGFRASTFPPTFHGWGE
                     IDTAQQTVAGWAVDSAHPWSRVEVQLVLDNELVATQVATLSRPDVAKAGWTRDEWHGY
                     AFKLPALKRGAHEARVYAVHKSAGRYTMQLLGDPIRFSIDSDGTARDVPVRSR"
BASE COUNT         1529 a         1480 c         1669 g         1474 t
ORIGIN      
        1 agtttcgaag aaggtggtgg tgaagaaccg gttggtgagg aagctccccg cgacgctttt
       61 gaagagatcg actttggacg tgaattccag gactatcttg atcctggtta taagacccag
      121 gagattgagt acaaagaaga cgctcccaca ttcgagcagt ttctaacgcg tccaccatcg
      181 ctggcagaac acctcgaatg gcagttgcac atgagtccta tcaagagcga tgtgtgcgac
      241 gctgcaatat gcgtgatcgg aaatctaaat gccgacggcc gtctcaacgc gacaaatgaa
      301 gagatggctg ccatggagaa atgttctgag gaagtagttg aggaggcgcg ccaggctatt
      361 atgcgtctcg atccggttgg ttgtggcgca cgcgatgtgc gcgagtgctt actggtccaa
      421 ctcgaagtgc tgggtcaaaa ggatcgcctg gcagcgaccc taatttccga acatctttcg
      481 gatctgcagc agcacaagct cccacacctg tcgaaacaaa ttggaattga tgtagaagtt
      541 ctacttgaag agctgcaatt cattcgcaca ctcgatccat acccgggtcg ccgttactcg
      601 tctgacgagc caatattaat tagccctgag atctacatcg agaagctcga cgaaaacgac
      661 gacgagtaca ttatctactt tgcggatgat ggtagccctc gtttacgcgt gagccagcag
      721 tatcagcaga tgcttagcca gggcgtgagc aacgagacaa agagtttcat aagagagaag
      781 atgcgttctg cagttgacct tctgcggaac atcgagcacc gacgtcagac gatttacaaa
      841 gtcgtggaat cgattgttca aaggcaacat gagtttctgg atcatggagt gcaaagtttg
      901 aaacccatga tgttgaaaga cattgccgaa gatatcggta tgcacctctc aactgtctct
      961 cgagttgtta atcggaaata cgctcatact ccacagggtg taattgagct gcgacgattt
     1021 ttcaccgagg gaatgctgaa cgaagatggc gaggaaattt caactcgaat cataaaactg
     1081 caaattaaga agctcattga agaagaagat tcgcataatc caattactga tgatcaggta
     1141 gtcaaaatcc tggctaaaga tggaataaaa ttatcgcgcc gcaccgtcgc caagtatcgt
     1201 gatcagatgc aaattcctgg ttcgcgcgag cgccgtgccg ttgtctgaac gccccccagc
     1261 gcggtttaac aatttcaagt acgtatcatg ataaccattg cgcgtagcct tccgtttcgg
     1321 gtcaggcttc gtatgcacgt ttgtcactgc tcgaggaggg agttggctga tgaaatttga
     1381 atatactggt cggcacgtcg atgtgtctcc ggctatccga cgacacgtgg aaggtcattt
     1441 caagaagttg gaccatatct tcaccgattc aacgcttagc actcatataa tcatcgacgt
     1501 agaaaagaac cgtcagatag gagagatcat tgtttactgg cgcgaacata cgctgacggc
     1561 gaaagatacc aacgcagata tgtacatggc cctgacgcgg gcgattgcca agatcgaaaa
     1621 gcaggctctt aaactcaaga aaaagatcat tgaccggaaa cagggtggcc agtcgactgg
     1681 tcgtgtggcg ccagatcccg atggccacct ggaggcaact ccccgaccac cgcgaattat
     1741 cgccgcccga agatatacag tgaaaccgat gactgctgaa gaagcggcgc ttaggttatc
     1801 gggcgaacca gaccagttcc tcgtatttcg tgatgctgat acctcgcgac tcggtgtgtt
     1861 atataaaagg aaagacggta attacggtct catcgagcct tagaagagcc cagtcaaaaa
     1921 cggacaactt attgcgtttt gaggtactct tgatttcgag cggaataagt tgtccacccg
     1981 caacgaaagc gatcaaccca ggtcgagagc gcgtcgcagc gaatggcccg taatccgaca
     2041 atccaacaac cccctgccag ttcgaaccac gttcccgata ttgtaattat taccggcctg
     2101 agcggttctg gtatgtcttc cgctaccaat gcctttcagg atctaggtta cttctgtgtg
     2161 gataatcttc ctcttacgat gttgcagacg tttgcacggc tgattacttc ggaagacgat
     2221 gaaaaggtaa ttcgtcgcgc cgcactggtc atcgatattc gagagggagt cttccttgcc
     2281 gagtttgaaa agcaactcct ggcgctacgc ggttctggtc taactgttac ggttctgttt
     2341 tttgaagcgt cagacgatgt tctgcaacac cggttcagcg aaaccaggcg ccctcatcct
     2401 gccgagagag ggcatgggtt actcgaagca atcagttctg agcgtgaggc gatggcgagc
     2461 attcgcgccc tggctgatca gataatcgac acctcggacc acactgttca cagtcttcgc
     2521 cgcttcttac ttgatcgttt cagtccggag ctggcaggcg cgccgatgcg tgttcaaatc
     2581 ttgagctttg gacacaagta cggcaatcct ggcgatctgg aactgttgtt tgacgttcgt
     2641 catctgccta acccacactt cgtgcccgaa ctcaaacctc tgtccggcca tgacagtcgc
     2701 gtagtgaaat acctgcgctc aaatcaggaa gttcaggaga cgatctcacg attcaccgat
     2761 ttgttgactt atctgttgcc gctttataaa cgggaaggaa agtcgtacgt taccgttggc
     2821 atcggttgca ctggaggacg tcaccgttcg gtcatggtgg caaatgcgct tgcaagagct
     2881 ctaagacgcg ctggatttga agtgcacgcc cgacaccgtg acgtgaggaa gggagctagt
     2941 cgcgggcgat agaaaggact cattggtcat atcgtttgat tgcttgtgga gaatctaacg
     3001 aggtttagta attatgaaat acgaattgag aattacgaat ggataagcag gaaattgaga
     3061 attaacaatt cagaattgag aattagatga gtaatacttt gcaaaatacg ccccagcctt
     3121 cggatgtaaa gcgaacctca ggcgtaatcg tcacccacgg acatttagcc ggcgagctgc
     3181 ttgccgccgc cgaaatgatt attggaccaa tctctcacat tgcagcagtt tctatcgggt
     3241 ggcatgacga cgttgatgcg gcacgagacg aggttcagcg tgcaattaat cgagtgtctc
     3301 agggccgtgg cgtcctactg ctcacggata tgttcggagg taccccgacg aatatcgctt
     3361 ccatgtttct ggaagaaaat gccgtcgaag ttgtcaccgg agtaaatctc ccaatggtta
     3421 ttaagctggc gacatcgccc gctgaagaat ctctggcaga aatcgctcgc aagatctgcg
     3481 atcaaggccg gcagggaatc tatctcgccg gagcattgct cgcacctccc tctaagaccg
     3541 gagccgattg aagtgactga acgaaagctt agagtcactg gtcgccttgg cctgcatgcg
     3601 cgagcggctg cgaccctcgt caggatagcg acacaatttc gtagcgagat aaagctccaa
     3661 cgtgtggacc gtggtggaga tcaagctgaa gccgctgatg cgaaatcgat cttaagtatc
     3721 ttaagtcttg cagccagttg cgggactgag cttttgctaa ccgctgaagg cgttgacgag
     3781 gagcaggctg tcaatgaaat cacggttctg ttcatccgac aatttgacga aggcgagcca
     3841 atcgaggtcg agcgccacgc ctcgacaatc ccggagttcc gagttaaggg tttgggtgtt
     3901 tctgacggcg tagcagttgg acgggtttta cgcgttcagg gagccaccag cgatctctat
     3961 cgggcttcaa ttgacgaaca ggatatagaa cgtgaacgca gaagattccg ggcggccgta
     4021 agagcttcga gaacccaact cgagtcaata aaaaagcgag ccgaaaggga actgggcaga
     4081 ggccacgctt atatctttga tgctcacctg ctttttcttg acgatgcaaa gttgacgcgt
     4141 gatgtggaag actacatcgt taaggaacat gcaaatgcgg aatgggcagc acgcgtcgtt
     4201 ggcgatcggc tgttatccat ttatacacaa attagcgatg agtatttgcg cgaacgcggc
     4261 tccgatattg aagacgtgat tcaacggttg ctcgcaaact tgaccggaga agagcgcacc
     4321 tatcccagtc tgtccgaaga cgctgtcatc gtcgcccagg acatactgcc atcaacagta
     4381 gccgagttgg atctgaagta cgtgaaagcg atcgcgaccg acgcaggggg ttggacttcg
     4441 cacatgtcga tcatagcgcg cggacttggt ttgactgctg tcgtagggct gagggatttt
     4501 tatcaccgcg cgcgtactgg agaaaagatt atcgttgacg cacgccgggg tgaggtcatt
     4561 cttcatccgc tgccggcgac catcgatcaa tatcaatttt cgacggaatc gataccggcg
     4621 ccttcacccg ttaaggaaga ggctattttt ggtcctgtga aaactcgaga tggagtaagt
     4681 atcaccctcc gagcaaatgt cgagctgccg actgagtttg aagcggttcg gagatttggg
     4741 gcttctggaa tcggtctgtt tcgctcggag tttttgcttt cacgccctgg actgatgctg
     4801 tccgaagaag accaatacct tgcttatcgc agattgattg aagcagccgg tgaacatgga
     4861 gccgtagtca gattattcga cgtaggcggt gagattgggc gagacctctc ggagcgcctt
     4921 gaacgcaatc ctgcccttgg attacgggca gttcgctttg atttaaggca tcccgaagtg
     4981 atgcgaactc aggtgcgcgc aatacttcgc gctgccactg caggaccctt aagccttgtg
     5041 cttccgatgg ttgcggatgt agcagatgtt cgacaggcta agagcgtgat cgaacacgag
     5101 cttagtgata tgcgagccac cggtatcgcg gtttcacccg tgcgtgtagg cgcaatgatt
     5161 gaagtgccat ccgctgtgct gacctcagaa aaaattgctc gtgaggtcga tttctttgaa
     5221 ctcggaacaa acgatctggt ccaatatacg ctcgcagttg atcgtggtaa tcatgaagtc
     5281 tccgattggt ttcgaactct tcatcctgcg gtcctatacg aaattgatcg cactttggcc
     5341 tcagcgcgtc aggcaggcat tcccgttatc gcttgtggtg aaatggcgtc tactcctgcc
     5401 tatgcggttt tactaattgg aatgggttca gttgatctga gcatgacccc ggctcttatc
     5461 ccgcgcgtgc gctcaatact cagtcagatt gatgccagcg cggccgagga aattgcgaga
     5521 aggtgtttgg gctgtgcgac ggcggatgaa gtcgaggaga tcgttcgagt tgaattcaga
     5581 cgtagatggc ctgacttatt tccgcccaag agtctaccgg cttaaatgaa aacatcaccg
     5641 gcacgcctga tacatgtttt gattggaaaa gctgtactcg aaacaatttt tgtcgctgct
     5701 cttgctttag gatttcgagc cagcaccttt cctccaacgt tccatggctg gggtgagatc
     5761 gatacagctc agcagacggt cgcaggctgg gccgtcgata gtgctcaccc atggtccaga
     5821 gttgaggttc agttagttct ggacaacgag cttgtcgcaa cacaagttgc cactctttcg
     5881 agacccgacg tagctaaagc aggttggact cgagacgaat ggcacggtta tgcatttaag
     5941 ttgccggcgt tgaagcgcgg ggctcatgag gcaagggttt atgccgttca taagagtgcc
     6001 ggtcggtaca caatgcagtt gttgggggat ccaattcgtt tctcaatcga cagcgacggt
     6061 acagctcgtg acgtgccggt tcgctccagg tagagtctta tggcgacaga cactcttaag
     6121 aacgaaaatt ggaccatcag cagacgcgcg gt
//
LOCUS       DASSFP010000467         7207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_743896, whole genome shotgun sequence.
ACCESSION   DASSFP010000467 DASSFP010000000
VERSION     DASSFP010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743896"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2443)
                     /gene="dnaE"
                     /locus_tag="VFH91_10505"
     CDS             complement(<1..2443)
                     /gene="dnaE"
                     /locus_tag="VFH91_10505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6863474.1"
                     /translation="MQDRQFVHLHLHTDYSLLDGAIQIKPLSKRIEELGMPACAMTDH
                     GNMYGAISFYNAMKSKGVKPIIGCETYITRGSRTEKAAAAPGEKANFHLILLAQNYEG
                     YQNLVRLTSKAYTEGFYYKPRIDKELLAQHSKGLIALSACMSGVPSALLAQDKCDEAA
                     KAALEFEEILGKGNYFLEIQEHGLDAQSRIRKSLIDLSKRTGVPLVATNDAHYLMPDD
                     ARAHDVLLCIGSGKTVNDSNRLRYASPNFYVRSPDEMWQIFGNEIPEVLTRTVEIAER
                     IDLKLPENINHLPRYPIPASDQGLSADQYFEKVVREGFERRRQKVWEPQLSRGELKQP
                     LSDYQTRLSSEIQMINQMGFAGYFLIVWDFVRYAKQNSIPVGPGRGSAAGSLVAYCLE
                     ITDVDPIQYDLIFERFLNPGRVSLPDIDIDFCVRGRGDVINHVADLYGRDSVCQIITF
                     GTLASKAAIKDVGRALEVPYSEVERIAKMIPPPVRGRNVSISQALEQVPELKREMETN
                     AQVKEILEIAKRLEGCARHSSVHAAGVVISPLPLQELIPIAVSGKDELTTQYVMSDLE
                     KTGMLKMDFLALTALTVINDCLISIKHMLGTEINWSDIALNDAKTMAVFAEGRTEAVF
                     QFESSGMQEICRKLKPKGVEDLAALNALYRPGPLDGGMVDEFILRHHGKKSVRYLVPE
                     MKDILSNTYGIIVYQEQIMQLAQRLAGYTLSEADLMRRAMGKKKREEMAVHEEKFING
                     AIERGIKKEKAEKIFSLMAQFSDYGFNRSHSVAYAYLAFQTAYLKAHFPEHFYAAVLS
                     SEAQDAAKVFKYSKEL"
     gene            complement(2537..4093)
                     /gene="guaA"
                     /locus_tag="VFH91_10510"
     CDS             complement(2537..4093)
                     /gene="guaA"
                     /locus_tag="VFH91_10510"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET6863475.1"
                     /translation="MNNALQHETVIILDFGSQYTQLIARRVREAGVYCEILPFNSSID
                     TILARNPKGLIFSGGPSSVYDANAPKPDPTLLQSVSCPTLGICYGFQVFARELGGAVA
                     ASPNREYGYARLKVVDVSSRLFQGLPAEMDVWMSHGDHVTEIPPGFRVTALTDDALNA
                     MESETGSIFGVQFHPEVAHTPLGAQILRNFLFSVCNCRGDWSPEVVIEEQVHRIRNQV
                     GENGRVVSGLSGGVDSTVAAALVHKAIGDRQTCIFVDNGLLREGEFESTLSLLGQTMK
                     LNIAGIRAETLFLEALKGISDPEEKRKRIGRVFIEVFEREARKVGDAQFLVQGTLYPD
                     VIESVSVRGPSAVIKSHHNVGGLPEKMNLKLIEPLRELFKDEVRIIGRELGVPQEILG
                     RHPFPGPGLAVRIIGDVTEDKIKLVQAADRILDEELRRAELYNAVWQAFPVLLPVSTV
                     GVMGDERTYERVVAIRAVTSVDGMTADWARLPHDLLARVSARLVSEIRGINRVVYDIS
                     SKPPSTIEWE"
     gene            4206..5348
                     /locus_tag="VFH91_10515"
     CDS             4206..5348
                     /locus_tag="VFH91_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6863476.1"
                     /translation="MLISRKQIITVFILLLTVSVCFCWRAQSSVSAQNFDLILSQPLT
                     VRWRYASNVTLNLTPAFDNERVYLPLAGGTLVALKAIDGQLFWRSDIGGELSASPIAD
                     NATIYVASEATALPGSPPATSGTLRALGREAGVTQWMTPLVRPLRGALALSGERIFGG
                     GSDGRVYAFDKRTGGVFWSIPFGAAFSGQPVIQGGRVYLGSEDGTLYSLEEATGRLLW
                     RYKTKGAIRGPVAIANGMALFGSGDGYVYAVSADKGKLVWQKRTGGGVEAVSLAGDAL
                     LAASLDNFAYLLKLNGKMIWKRQLPGRISSQPLTIQEAALFTPLSSSNAVVLALRDGK
                     QVNSLATGEELTSSAGPIIVGDIVMVTTEHGLLAFSRPLTSTKPAP"
     gene            complement(5345..6274)
                     /locus_tag="VFH91_10520"
     CDS             complement(5345..6274)
                     /locus_tag="VFH91_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HET6863477.1"
                     /translation="MSSARMIVEDERPNPPRRGLKKGLYLIPSLFTAANIAMGFFSVM
                     ASVRGFQLLAGSSPDVIGAAAHFDSAAIAIGLAVVFDMLDGRIARMTRTTTEIGIQLD
                     SIADVVTFGLAPAVLAYVWGYGASLAEGTKLHQLAWFLSFMYLMCGAFRLARFNVQAS
                     RPRILAEGTPKVDKKSFVGLPIPVAGGLIAAIVHFAPEPLMSLGPDKAKIYSGLLMTL
                     VGFLSAMMVSTLRFSSFKTVGTRTRSMRTIILVLAVGMLIFLYSQYVLLIIAIAYILH
                     GLGSRVVGMIWRHGETPEKIVATNTVSRSSSRS"
     gene            complement(6271..6891)
                     /locus_tag="VFH91_10525"
     CDS             complement(6271..6891)
                     /locus_tag="VFH91_10525"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HET6863478.1"
                     /translation="MSIVRESIPYVTVPLLICAILAIFGLWYVAIFFLAIALFMAFFF
                     RDPHRVPPDSPDVIVSPADGRVTRIKKLAAEDQNSATLVSIFLSPLDVHINRAPISGR
                     IVDMVYSPGKFLMATNEQASLVNEQNALTIQGEKVTVVCKQIAGILARRVVCWKKKGD
                     NLQLGERFGMIKFSSRTDIVLPPNVVVTVTEGSKVRGGSTVIGRIS"
     gene            complement(6998..>7207)
                     /locus_tag="VFH91_10530"
     CDS             complement(6998..>7207)
                     /locus_tag="VFH91_10530"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HET6863479.1"
                     /translation="ELINRDPEFRELAREHHKYEERLSELSALSYPSDEEQLEEVTLK
                     KKKLALKDQMYSIMLQYQKAHTVSH"
BASE COUNT         1767 a         1832 c         1787 g         1821 t
ORIGIN      
        1 tcaactcctt tgaatactta aagactttag cggcatcctg agcctcgctg gacaggacag
       61 cggcatagaa gtgttctgga aaatgagcct ttagataagc ggtctgaaaa gcaagatagg
      121 catacgcaac gctatgacta cggttgaagc catagtcgga gaactgcgcc atcaacgaga
      181 agatcttttc tgctttttct tttttaatgc cccgctcgat ggccccattg atgaatttct
      241 cctcatgtac ggccatctcc tcgcgcttct tcttgcccat cgcgcgtcgc atcaggtcgg
      301 cttcactcag cgtataaccg gccaatctct gcgccagctg catgatctgt tcctggtaga
      361 caataatccc ataggtgttg cttaggatgt ctttcatttc aggtaccaga taacggacgc
      421 tcttctttcc gtgatgcctc aaaataaact catctaccat tccgccatcg agagggccgg
      481 gccggtaaag cgcgttaagg gcggccaagt cttcgacacc cttcggctta agcttacggc
      541 agatctcctg catgcccgaa gattcgaatt gaaagaccgc ttcggtcctt ccctcggcga
      601 aaacagccat tgttttagcg tcgttaagcg ctatatcact ccagttaatc tccgtaccaa
      661 gcatgtgctt aatgctgatt aagcagtcat ttataacagt taaagcagta agcgctaaaa
      721 agtccatctt aagcatgccg gtcttttcca gatccgacat gacatattgg gtagtgagtt
      781 cgtccttccc tgagactgcg atgggaatca attcctgaag gggtaatggg gagataacta
      841 cacccgcagc gtggacggat gagtggcgag cacaaccttc aagtcgtttc gcaatctcaa
      901 gtatttcctt aacctgagcg ttggtttcca tctcacgctt tagttcaggt acttgctcaa
      961 gggcctggct aatgctcacg ttcctgccgc ggacaggtgg cggaatcatt ttggcgattc
     1021 gctcaacttc cgaataagga acttcaagtg cgcggccgac gtccttgatt gcggccttag
     1081 acgcaagggt cccaaaagtg atgatctgac agactgagtc gcgtccgtag aggtcggcga
     1141 cgtgattgat gacgtccccg cgaccacgaa cgcaaaagtc gatgtcgata tcaggcagtg
     1201 acactcgacc cgggttaaga aagcgctcga agatgaggtc gtactggatt ggatcaacgt
     1261 cagtaatctc gaggcagtag gcgaccagcg acccagcggc tgagcctcgg cccggaccaa
     1321 cagggattga attctgtttc gcgtagcgga cgaagtccca gacgatcaga aagtacccgg
     1381 caaagcccat ctgattaatc atctggatct cggaactgag tcgcgtctga taatcggaaa
     1441 gaggctgttt cagttccccc ctcgacagct gtggttccca caccttttgc cggcgtcgct
     1501 cgaatccctc acgaaccacc ttttcaaagt attgatccgc actcagcccc tgatcggatg
     1561 cagggatggg ataacgcgga agatggttga tgttctcggg cagctttaga tcgatgcgct
     1621 ccgctatttc aactgtacga gtcaagactt ccggtatttc attgccgaag atctgccaca
     1681 tttcgtcggg cgaacggacg tagaaattcg ggcttgcata gcgaaggcga ttcgaatcgt
     1741 tgaccgtctt ccctgagcca atacacaaga gtacgtcgtg agctcgcgca tcgtctggca
     1801 tcagataatg agcgtcattt gttgctacca gaggtactcc ggttcgtttt gaaaggtcta
     1861 ttagagactt tctgatccga ctttgagcat caagtccgtg ctcctgaatc tcgaggaagt
     1921 agttcccttt tccgagaatt tcttcaaatt cgagcgcggc ctttgcagct tcatcacact
     1981 tgtcctgggc cagcaaggca gaaggaacgc cggacataca cgctgacaat gcgattaaac
     2041 ctttgctgtg ttgcgccagg agctccttgt cgatgcgggg cttgtaatag aagccttccg
     2101 tgtacgcctt tgaagtgagt cgcaccagat tctgatatcc ctcgtaattc tgggccaata
     2161 ggatgagatg gaaatttgcc ttctctcctg gtgctgcagc ggctttttcg gtgcgactac
     2221 cgcgagtgat ataggtttca cagccgatta tgggcttcac acccttactc ttcatcgcat
     2281 tgtagaagga gattgcccca tacatattgc cgtgatcggt catggcacat gccggcatcc
     2341 ctaattcttc aatccgtttt gagagaggct tgatctgaat agcgccgtcg agaagactgt
     2401 aatcagtgtg gaggtgtaag tgtacgaatt ggcggtcctg cattagttat aagtgtgcct
     2461 gctcattcgt gagcatggag atcatttctg gttgacgtag gagaagatat attacaacac
     2521 gaagctgacg ttagttctac tcccattcga tcgttgaggg aggcttagaa ctgatgtcat
     2581 acacaactct atttattcct ctgatttcag aaacaagacg tgcggataca cgtgcaagca
     2641 gatcgtgtgg caaacgcgcc caatcggccg tcatgccatc cacactggta actgctcgaa
     2701 tggcgacaac gcgctcataa gttctttcgt cgcccatcac tccgactgtc gataccggta
     2761 agaggactgg aaaggcctgc cagaccgcat tgtaaagttc agctcgcctt aattcttcat
     2821 cgagaattct gtccgccgcc tggaccaatt tgattttatc ttcagtaacg tcgcctataa
     2881 ttcgaacggc cagtccggga cccgggaaag gatggcgacc gagaatctct tgtggtacac
     2941 ccagctctct tccgatgatt cgaacctcat ccttgaataa ttctcgcagc ggttcgatca
     3001 gcttgaggtt catcttttca ggcagtccgc cgacgttatg atgactttta atgaccgcag
     3061 atggaccacg aacagaaact gactcgatca cgtcagggta taaggttccc tggaccaaaa
     3121 actgcgcatc tccgaccttg cgagcctcgc gttcaaaaac ttcgataaaa actctaccga
     3181 tgcgctttcg cttctcttcc ggatccgaaa taccttttaa agcttcaagg aacaatgttt
     3241 cagcacgaat tccagcaatg ttaagcttca ttgtctggcc caggagcgaa agggtcgact
     3301 cgaattctcc ctcgcgtaaa aggccgttat cgacaaagat acaggtttgt cgatcaccaa
     3361 tcgccttatg taccagcgca gccgcgacag tcgaatctac gccacctgac aggcccgaaa
     3421 caacgcggcc gttttctccc acttgatttc gtattctgtg gacctgctcc tcaatcacca
     3481 cttcaggtga ccagtcgccg cggcagttgc aaacagagaa gagaaagttg cgaaggattt
     3541 gagctccgag tggagtgtga gcgacttcgg gatgaaattg cacaccaaag atgctgcctg
     3601 tttcactctc catcgcattc agcgcgtcgt ctgttaacgc agtcacccta aaacccggcg
     3661 gtatttcagt gacgtgatct ccatgactca tccagacatc catttcagcg ggaaggcctt
     3721 gaaacaaacg actgctgacg tcaaccacct ttagtcgcgc ataaccgtat tcgcggttag
     3781 gagaggctgc gacggctccg ccaagttctc gtgcaaaaac ttgaaacccg tagcagattc
     3841 cgagcgttgg gcaactaact gattgaagta gtgttggatc aggttttggc gcgttcgcat
     3901 cgtaaactga ggacgggcca cccgaaaaaa tgagcccttt cggattacgc gccaaaatgg
     3961 tgtcgattga tgaattgaat ggaagaattt cgcaatacac acctgcttct ctaacgcgac
     4021 gtgctatcag ttgtgtgtac tgtgatccga agtcaagtat tattaccgtt tcatgctgga
     4081 gggcgttgtt caagaaaacc tctggccttg tttgcgagtc aaatagagtt tgaagagtgg
     4141 cagattatac ttttggatcc taataaacgt aaaccaggat tggcctaatt ttttcggttg
     4201 aatttatgct gatttccaga aagcagataa ttacagtttt tattttattg ttgaccgtgt
     4261 cagtttgttt ctgctggcgc gctcagtcga gcgtaagtgc tcaaaatttc gacttaattc
     4321 tctctcaacc acttactgtg cgttggcgat atgcgtcaaa tgtcactctg aatcttactc
     4381 cggctttcga caatgaacga gtatatctac ccctcgccgg tggcacactc gtggcactta
     4441 aggcgattga tgggcagctt ttttggcgat cagatatagg cggtgaattg tctgcgtctc
     4501 caatagctga caatgcgacg atctacgttg cgtcggaagc tactgcgcta ccaggctcgc
     4561 cgccggcaac ttcaggaacg ttgcgcgctt taggacgaga ggctggtgtg acgcagtgga
     4621 tgacgccact tgtgaggcct ttgcgaggag cactcgctct gagtggcgaa agaatttttg
     4681 gtggaggcag tgatggacga gtgtacgctt ttgacaaacg tacaggcgga gtcttttggt
     4741 ccatcccgtt tggcgccgcc ttcagcggcc agccggtgat ccagggtggt cgtgtatatc
     4801 tcggttctga agacggaaca ctttacagtc tcgaagaagc gaccggccgg ctgctctggc
     4861 gctacaaaac aaaaggagca attcgtggtc cagttgccat cgctaatggt atggccctct
     4921 tcggttccgg tgatggctac gtttatgcag tgtccgccga caagggaaaa ctggtctggc
     4981 aaaaacggac tggcggcgga gttgaagctg taagcctggc tggcgacgca ctactggcag
     5041 cttcgttgga taactttgct tatttgctta agttgaatgg aaaaatgatc tggaagcgac
     5101 agttgcccgg acgtatatcc tcgcaacctt tgaccattca ggaggcagcc ctgttcacgc
     5161 cgctttcgag ttcaaatgcc gttgttttag ctctcagaga tgggaaacag gtgaattccc
     5221 ttgccactgg ggaggaacta acgagctcag ctgggccaat catcgttggc gacattgtaa
     5281 tggtgactac tgagcacgga ttgcttgcgt tctctcgtcc tcttacaagt acaaagccag
     5341 cgccctaact tcgtgatgac gacctgctga ctgtattagt tgcaacgatt ttctcgggtg
     5401 tctcaccgtg tctccagatc atgcccacga cacgtgaacc aagaccgtgc aagatataag
     5461 caatcgcaat gatcaggagt acgtattgag agtaaagaaa aatcagcatg ccgacagcga
     5521 gaaccaggat tatggtgcgc atgctgcgag tgcgtgtccc gacagttttg aagctcgaga
     5581 atcgaagtgt gctcaccatc atcgcactca agaacccgac gagagtcatc aatagtcccg
     5641 aataaatttt tgctttatct ggacccaaac tcatcagagg ttcaggcgcg aagtggacga
     5701 ttgccgctat caagccacct gcaacgggga ttggtaagcc gacaaaactc ttcttatcta
     5761 cttttggcgt tccttcggca agaatgcgcg gtcgggatgc ctgtacgttg aagcgcgcca
     5821 atcgaaacgc gccacacatc aagtacatga atgacaaaaa ccacgcgagc tgatgaagtt
     5881 tagtgccctc agcaagactc gcaccataac cccaaacata agccagcaca gcaggagcca
     5941 ggccaaacgt aacgacatct gcgatcgagt cgagttgaat accgatctcc gtggtagtgc
     6001 gagtcatgcg agctatccga ccgtcgagca tgtcgaacac aacggccagg ccaattgcga
     6061 ttgctgcact atcgaaatgc gccgctgccc caattacatc tggtgacgat cctgcgagta
     6121 gttgaaagcc tcttaccgag gccatcacgg agaagaatcc cattgcgatg ttcgcggccg
     6181 tgaagagcga aggaatgaga tagagtcctt ttttaaggcc acgtcgcggt gggttaggac
     6241 gttcgtcttc tacaatcatt ctggctgagc tcatgagatc ctcccgataa cggtggaacc
     6301 tccacgcact ttactgcctt cagtaacagt gacgacgaca ttgggaggca acacgatatc
     6361 cgtgcgcgaa ctgaacttaa tcattccgaa gcgttctccc agttgcagat tatcgccctt
     6421 cttcttccaa caaaccaccc tccgagcgag gatcccggcg atctgcttgc aaaccacggt
     6481 caccttctcc ccttgaatgg tcagggcgtt ctgctcgtta acgagactgg cctgctcatt
     6541 ggtggccatc aggaattttc ccggcgaata gaccatatcc acaatcctgc ccgaaatcgg
     6601 agcacgattt atatgaacat ccagcggcga gaggaaaatg cttacaagtg tcgcagaatt
     6661 ttggtcttca gctgcaagct ttttgattct cgtgacccga ccgtctgccg gcgaaactat
     6721 gacatccggg ctatctggag gaacgcgatg tggatcgcgg aagaagaaag ccataaacaa
     6781 cgcgatcgcc agaaagaata tcgcgacgta ccagagtcca aaaattgcca aaatggcgca
     6841 tatcaacagt ggtactgtga cgtatgggat gctttctcga acaatactca aaacggagtc
     6901 ttaatctaat caaatgagat cttgctatat aaaaaaggga cgcgatttgt gttccacgtc
     6961 cctttatctc caaaactggg aagattaacc ctgctgctta atgactcacc gtgtgagcct
     7021 tttgatattg cagcatgatc gaatacatct gatcctttag ggcaagcttc tttttcttaa
     7081 gtgtaacttc ttccaattgt tcctcatcac tcggatagga aagagctgat aattcactaa
     7141 gacgttcttc gtatttatga tgttcgcgtg ctagttctct gaattcgggg tcccggttta
     7201 tcaactc
//
LOCUS       DASSFP010000468         5162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1038354, whole genome shotgun sequence.
ACCESSION   DASSFP010000468 DASSFP010000000
VERSION     DASSFP010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038354"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1680)
                     /locus_tag="VFH91_10535"
     CDS             complement(337..1680)
                     /locus_tag="VFH91_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET6863480.1"
                     /translation="MIITRKRLPRRTFLRGMGTMVAIPMLDAMVPAFASSRDSELKAP
                     ARMAFAYVPNGIVMKEWTPKTTGSSYEFTRILKPLEAFRDDILVLSNLDSNTGNALGD
                     GPGDHARAGASFLTGVHCRKTAGADIRGGVSVDQVAATKFTGATRFPSLELGCEDSRT
                     VGDCDSGYSCAYTNSLSWRTPQTPMPPEVNPRAVFERLFGTAEDLSLDAETRARRFRY
                     RKSILDLVTRDTQSLVKTLGPGDRRKLDEYLFSVRQVEQRIESVEKDPRRPAPGIDKP
                     SGIPFEFPQYVKLMCDLQVLAFQSDLTRVVTLVIGREGSNRVYPEIGIDDPHHPLTHH
                     RNNPEWIEKVTRINTLHTEMFAYLLKRLKSTPEGDGTLLDHSLLLYGSGLADGNAHTH
                     EALPVLLAGRGGGVKPGKHEIAKQGTPITNLYLSMLDRMGVHQDSLGDSSGMFEL"
     gene            complement(1680..4223)
                     /locus_tag="VFH91_10540"
     CDS             complement(1680..4223)
                     /locus_tag="VFH91_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET6863481.1"
                     /translation="MKISLTRLIPLFLCPALLVSLLAVTHQITTVESKSQDPPKDDPE
                     FETVVKPFFAKNCFACHDEDLQTANLNLGAFKTASSLTQQRATMKLILDKVKSGQMPP
                     AKMPQPKPEEVAALSSWLTHQLAQMPEQSASNSQSNAANSSPGRVTLRRLNRVEYNNT
                     VRDLLAVDRHLADDFPQDDSGYGFDNIGDVLSLSPVLMEKYLAAAEKISRTAIFGVEP
                     IKPTLVRLRSGERTHTPQLTPLTDYDQSGLSMPNAVHTTYRFPVDGEYVIRVNLGGDR
                     PAGSEAFQLSLWIDGEQKQTIRVDPGSLASFARKGEPQQLWGIRPEFRVTVKEGEHWI
                     GVAIPRLYEGLPPGYNGPNPSRLPIPPWPPYKPPPDLTPKEIQERRLEYEKKKLEKLP
                     ANSARVGFLELGGPYASSKGPSLESKKKIYVCGHLNGHHTDSCARMIIESMAHRAYRR
                     PVSTNEVLQLTNLVASVQKETGSFDEGLAVAIQAILMSPNFLFRVEKLPATTASASRK
                     LSQHELASRLSYFLWSSMPDDALLLAADRGRLARPDVLAAQVRRMLLDPKSQSLVDNF
                     GGQWLQVRKLESVKPDGKRFPEFDEYLRLSMARETQVFFSSIIREDRSILDFIDADYS
                     YLNERLARFYNVPNITGPEFRKVSFSPDSHRGGLLTQASVLTISSYANRTSPVLRGKW
                     VLENLVGTPPPPPPPDVPNLDEAKIGKTSSMREQLEQHRKNAICASCHSRMDPLGFGL
                     ENFDAIGAWRSKDGEFPIDSSGILPDGTSFSGPQGLKAILKQKPDAFAECLTRKLMVY
                     ALGRGLEPSDEGTVKEIVRNVSANEYRFSSLIMGIVKSQAFERRRGEKL"
     gene            complement(4466..5080)
                     /locus_tag="VFH91_10545"
     CDS             complement(4466..5080)
                     /locus_tag="VFH91_10545"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6863482.1"
                     /translation="MRTLMVLLILAATSALTLAVQSGRKISTARTAPTAPIQAPLTPE
                     PEPPVAASPVSLMSIPESYREREIRSLNKGSFKLSDFYGKVIVINLWASWCGPCRREV
                     PEYEQVRKSYAGQNVEFIGLTTEDPRSSSGSVNKFLREVNFGFRLGWADRDLARLLMN
                     GRGSIPQTIVLDSRARIVRQWAGYSAGHSGDRLTASIEEALKGG"
BASE COUNT         1262 a         1352 c         1308 g         1240 t
ORIGIN      
        1 atacccccaa cgggagttgg gggattgttc agatccaacc tacaagagcg catccacaag
       61 gatctcgaaa tacccccaac gggagttggg ggatggttca gatccaacct acaagagcgc
      121 agctcaacca ggacctcgaa atacccccaa cgggagttgg gggatttttc agatccaacc
      181 tacaagagcg cagttcaacc aggaccccca gaataccccc aacgggagtt gggggattat
      241 tcagatccag cctcggagca gttcagcaca agaaggaagt cacgtttgta gtttggactt
      301 gaaagatccc ctaactgccg ttagggggat tggcttttac aactcaaaca taccagagct
      361 atctcccaaa ctgtcctggt gaactcccat ccgatccagc atcgaaagat ataggtttgt
      421 aattggtgtt ccctgcttcg cgatttcatg tttgccgggc ttcactccac caccgcggcc
      481 cgcgagcagc accggtaaag cctcgtgcgt atgagcattg ccgtccgcaa gtccgctccc
      541 gtaaagcaac aaggaatggt ccaataatgt cccatcgccc tctggagtgg atttcaggcg
      601 ctttagaaga taagcaaaca tttctgtatg cagcgtatta atccgagtaa ccttttcgat
      661 ccattcagga ttgttccgat gatgagtcag cggatgatgt ggatcgtcaa tcccgatctc
      721 aggatagact cggttgctac cctcacgacc aatcaccagc gttacaacgc gagtaagatc
      781 tgattgaaaa gccaaaacct gcaggtcgca catcaacttc acatattgcg ggaattcgaa
      841 aggaattccg cttggcttat cgatacccgg cgcaggcctg cgaggatctt tttcaacgct
      901 ttcaatgcgt tgttcaacct ggcgaactga aaacaaatac tcgtccaact tccgacggtc
      961 gcctggtcca agagtcttaa caaggctttg ggtatcgcga gtaactaaat cgagaatgct
     1021 tttgcgataa cgaaaacgtc gagcccttgt ttcggcgtcg agacttagat cttcggccgt
     1081 gccgaaaaga cgctcaaaaa ccgctcgagg gtttacctca ggtggcatgg gcgtctgagg
     1141 tgtgcgccac gacagactat tggtataagc acagctgtaa ccagaatcgc aatcgccgac
     1201 agtgcgtgaa tcttcacaac ccaactcgag tgaagggaac cgggttgctc cagtaaattt
     1261 cgttgcggca acctgatcta ctgaaacccc tccccgaatg tcagcaccag cggtctttcg
     1321 gcagtgcacg cctgtaagaa atgaggcacc agctcgcgca tggtcacctg gtccatcgcc
     1381 gagtgcgttg ccggtgttcg agtcaagatt agacaggact aaaatatcat cgcgaaaggc
     1441 ctcaagcggc ttgagaatgc gcgtgaactc atagctgctg ccggttgttt tcggagtcca
     1501 ctctttcatg acaatgccgt tcggcacata agcaaaagcc attcgagcgg gtgctttgag
     1561 ttcactgtca cgactactcg cgaaggccgg aaccatcgca tccaacatgg ggatggctac
     1621 catcgttccc attccgcgaa gaaatgtgcg cctgggtaat cgtttacgcg taatgatcat
     1681 tacagtttct ctccccttcg cctttcaaac gcctgactct tcacaatccc cattatcaaa
     1741 ctcgagaacc gatattcatt agctgatacg tttctgacaa tctctttgac cgttccttcg
     1801 tccgaaggct caagccctcg tcctaatgcg taaaccatca atttcctggt caggcattcc
     1861 gcaaaagcgt caggcttctg cttgagtatc gcctttaatc cttgtggacc actaaacgaa
     1921 gttccgtctg gcaatatacc cgaagagtca atcgggaact caccatcttt tgagcgccac
     1981 gcgccgattg cgtcaaaatt ttccaacccg aaaccgagag gatccatgcg cgaatggcaa
     2041 gatgcgcaga tagcgttctt tcggtgttgt tccaattgct cgcgcatgga agatgtcttg
     2101 ccaatctttg cttcgtccag atttggcaca tcaggcggcg gagggggagg tggagtgccg
     2161 accagatttt ccaacaccca tttgccgcgc agtactggcg acgtgcgatt cgcgtaagag
     2221 gaaattgtca aaacacttgc ttgtgtcagc agcccgccgc gatgggaatc cggactaaac
     2281 gataccttgc gaaactctgg ccccgtgata tttggaacgt tgtagaacct cgccaatcgc
     2341 tcgttcagat aggagtaatc tgcatcgatg aagtcgagga tgcttcggtc ttcccgaatg
     2401 atgctcgaaa agaacacctg agtctcgcgc gccatcgaca gccttagata ctcatcaaac
     2461 tcaggaaaac gtttaccatc gggcttaacc gattcaagct ttctaacctg cagccattgt
     2521 ccaccgaagt tatcgaccag tgattgagat ttcgggtcaa gcagcatgcg acggacttgt
     2581 gcggcaagca catcaggtcg agcgagccgg ccacgatcag ctgcgagcaa tagcgcgtca
     2641 tcaggcatgc tgctccacag aaaatatgac agacgcgagg ctaactcatg ctgggaaagc
     2701 tttcgcgagg cagacgcggt agtagccggt aacttctcga cgcgaaaaag gaagtttggc
     2761 gacatcagta tcgcctggat tgcaaccgcc aatccttcgt caaaagagcc ggtctccttt
     2821 tgaacgctgg caaccagatt ggttaactgc agaacctcgt tggtactgac gggcctccgg
     2881 tatgctcgat gggccatgct ttcaataatc atccgagcgc agctatcggt gtgatgacca
     2941 ttcagatgtc cacagacata gatcttcttt ttactttcaa gcgatgggcc tttactcgag
     3001 gcgtatggac cacccaattc caggaatccc acccgagcac tatttgccgg aagtttttcc
     3061 agcttctttt tctcgtactc tagacgtcgc tcttggatct ctttaggagt gagatccgga
     3121 ggaggtttat acggcggcca tggtggaatt ggtaatctgg atgggtttgg cccgttgtaa
     3181 ccgggtggaa gaccctcata caggcgcggt atcgccacgc caatccagtg ttcaccctct
     3241 ttaactgtaa ctctaaattc cggacgtatg ccccagagtt gttgaggctc acccttccgg
     3301 gcaaacgagg caagactccc aggatcgacg cgaattgttt gcttctgttc accgtcgatc
     3361 cacagtgaga gctggaatgc ttctgagccg gcaggccgat caccgccgag attaactcga
     3421 attacgtact ctccgtcgac agggaatcga taggttgtgt ggaccgcatt aggcatgctc
     3481 aagcctgact gatcgtaatc ggtgagtggt gtaagttgcg gagtgtgagt tcgctcgcca
     3541 cttcgcaatc tcaccagcgt aggcttgatt ggctcaacgc caaagatcgc tgtgcgagaa
     3601 attttttccg ctgcggctag atatttctcc atcaacacag gcgacaacga cagaacgtcg
     3661 ccaatattat caaaaccata tcccgagtcg tcctgaggaa agtcgtccgc cagatgtcga
     3721 tcgacagcca ggagatcgcg gacagtgttg ttgtactcga cgcggttaag acgcctgagg
     3781 gttactcgtc ccggactaga attggctgca ttactctggg agttcgaagc gctttgttcc
     3841 ggcatttgtg caagttggtg cgtaagccat gaacttagtg ctgcgacctc ctcaggttta
     3901 ggctgcggca tctttgccgg tggcatttga ccgcttttaa ccttgtccag aatcagcttc
     3961 atcgttgcgc gttgttgcgt tagagatgaa gcagttttga acgccccgag attaagattc
     4021 gccgtttgca gatcctcgtc gtgacaggca aaacagttct ttgcgaaaaa gggcttaacg
     4081 accgtttcaa actccggatc atctttagga ggatcttgag atttcgattc gacagttgtt
     4141 atctgatgag ttactgccag cagcgatacc aacagcgccg ggcagagaaa tagtgggatt
     4201 agtctggtca acgagatttt cacagttcta agcaggcagg actggtctga agaatcccag
     4261 actgccttag cgcgcccggc gatcttattg caaatagaga atatagatga tcagaccgga
     4321 cgagggaaga ccggaatgcg aggtcttagc aaaaaatttt ttatagagta gtaaacgaca
     4381 aaatgtgagc cgatgtctgt cgcctgggat cgcttaatga caaccggctc ttcgtcaaat
     4441 ttggcccagc ctgggagacc aatcgtcagc caccttttag cgcttcttcg atcgatgcag
     4501 tcaatcggtc gccgctgtgt ccggccgaat aaccggccca ttgcctgaca atccttgcac
     4561 gactatctag cactatcgtt tgcggaatgg accctcggcc gttcatcaac agtcgcgcca
     4621 aatcgcggtc ggcccaaccc aggcggaagc caaagttgac ctcgcgtaga aatttattga
     4681 cgctccctga cgatgaacgc ggatcttctg tggtcaaccc aatgaactcg acgttctggc
     4741 cggcgtagga ttttcggact tgctcgtatt caggaacctc ccgacgacat ggtccacacc
     4801 atgaggccca gagatttatc acgattacct tgccgtaaaa gtcagaaagt ttgaagcttc
     4861 ccttatttag ggacctgatc tcgcgctcgc gatagctttc tggaatggac atcaacgata
     4921 ccggtgaagc ggcgactggt ggttcaggct ccggcgtcag tggtgcctgg atcggagctg
     4981 tcggcgccgt ccgcgctgta ctgatctttc ttccagactg gaccgcaaga gttaaggctg
     5041 aggtggccgc gaggatgagt aaaaccatta aggttctcat gattatttcc aaagttggat
     5101 ttgagacaac tggttgctga ggctttatat ataaagaaca ggcactgaac aagtcagaaa
     5161 tc
//
LOCUS       DASSFP010000469         8525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_744422, whole genome shotgun sequence.
ACCESSION   DASSFP010000469 DASSFP010000000
VERSION     DASSFP010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744422"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1104)
                     /locus_tag="VFH91_10550"
     CDS             complement(139..1104)
                     /locus_tag="VFH91_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863483.1"
                     /translation="MRTLRRYPAVKTFALLTLLAIVNSPANAQPQSVAGSNSNGTPGK
                     PVTIPLTINVKEAEHESELQNIDLTVSEDGEPQSIISIRGMGTNSPITLALLIQEDLV
                     PSVANETKALQEFIRSLPRGSRVMIGYLRTGSLQVKQKFTIDLEKAARSLRPPAGFAS
                     VGPYNPYVEVIEGLKRFDAQPLGRRAILLISDGLDISRGIDSSSPTQSLDLQRAITEA
                     QRRSVAIYGFYAPTLAAQGNFLLTSNAQSSLLKLSTETGGHAFFQGTGVPVSFDPFIR
                     ELDTSLNRQAALTFLSTHLKKGFHRIEVRSSTSGVKVAYPYGYVR"
     gene            1230..2018
                     /locus_tag="VFH91_10555"
     CDS             1230..2018
                     /locus_tag="VFH91_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863484.1"
                     /translation="MSEAIGKVSSRDVAVNRMLKVAPWLAVVVATLPAPLVFLLLFVT
                     STATDSAAVFLLLAGTSLTFGFATGLLIAVILLLYRRKWLARLRDRLASDGITANEVV
                     WFRSELTSAERASLAEIERTNPLLADAYLETLASRLTASRIISRARNEMLKVERRLNR
                     ARSLASADTESLQKELLADRERLAEVRQRASEHLTRAKARLQEIEATASRKLSVLETD
                     VMMQRLGSAQDQLPLVLEIARLEQQALLEANTQINDTPVKAGGN"
     gene            complement(2023..2835)
                     /locus_tag="VFH91_10560"
     CDS             complement(2023..2835)
                     /locus_tag="VFH91_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863485.1"
                     /translation="MSESVVTLPEEIESYRDRLWRRDPELRVENVFAAERFIEEVGFC
                     EALTDSRRPGPSLYVAVCGRRDAHMPRNVQKDAESSLTWTIKDEVMRRGRHFYGKLLK
                     GRATFVAPRLLPFFNSLLGIPAGREKSQLTNDARLVLKVLRSEWEMATRDLRDECGIG
                     DRARFNKALDELQKTLKVIPSDVIYQPTFSYIWTIAESRFPDQMKERVDRELAVKEIA
                     RAYLSGAGMTVRGELARVTGLRSPEAGLGNWALVDDGFARRLAPGVYRLAML"
     gene            3037..5061
                     /locus_tag="VFH91_10565"
     CDS             3037..5061
                     /locus_tag="VFH91_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6863486.1"
                     /translation="MKSINKPRRFLTLSVLTALSLAAIVPGSFLLSSASSAAGKNGKN
                     HKGGHHNKVGSDLREQMHGKHGGSFVNVILQLNSSPSGQLNALLRSNGVRVKGQFSNF
                     NSYALELPVSVVDSLAMFPEVEFVSVDSEVKTLGGHIAHTTGADNVRSMAFDGSLDGN
                     GVGIAIVDSGMYAAHTAFRDALTGLSRIVVSKDFTGEGRTDDPYGHGTHVAAAAAGSG
                     IVSSGEYIGIAPRANLINLRVLNSQGVGNVSSVLSALDWLMVNATSYNVRVVNLSLGM
                     PAINSSQFDPLCRAVRRLVDRGIFVVTAAGNNGKNSDGQKVYGQIHSPGNEASALTVG
                     AVDTHGTDSRGDDTVATYSSRGPTRSFWTDEFGVKHYDNIIKPEISAPGNKTIFAEAP
                     DNFILSQNPSLDAGVSGNVTRKQMILSGTSMAAPLAAGTAALLIEANPTLSPNLIKAL
                     LMYTAQQLPNYNMFEQGAGELNIDGAVRIAKLVRSDLNDLTPLGSNLINGSLPSQQTS
                     ITTSNGVSTFQWSRGIILGHTFATGVDLMTKYQRIYSHGVLLGDGVLIGDGVLIGDGV
                     LIGDGVLIGDSTMLSNGVLIGDNILVSNGTLMNSGSIFCAAGVLIGDGVLIGDGVLIG
                     DGVLIGDGVLIGDGVLIGDAVIQALNAMISGDATPSMQPIPDNEIDYLGF"
     gene            5110..6021
                     /locus_tag="VFH91_10570"
     CDS             5110..6021
                     /locus_tag="VFH91_10570"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6863487.1"
                     /translation="MLETSGTAQLNNRSMGIGSVDAGNVTRLRTGQETEVLNFLSERP
                     IHTVAMMSMIRDNGLTSPFNRGVFYGYRDLNGRLEGVALIGHATLLESVSDRALSALS
                     RVARKCGAIHMIMGEQDRIDEFWSSYSEEGHKKRHSCIEQLFELSTPMEFPGETDRLR
                     LATADEVDLVMPVQAVLAFEESGVNPMKSDPEGFRLRCLRRIQQGRTWVVVDNGALVF
                     KAEVISQTSGVAYLEGIWTRHDKRQNGDSVRFVSELSRKLLQEVNSICLLVNEKNLLA
                     KAFYRKCGFLFRASYKTIFLKTNERSN"
     gene            6092..8062
                     /locus_tag="VFH91_10575"
     CDS             6092..8062
                     /locus_tag="VFH91_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6863488.1"
                     /translation="MSNTPGTKRNPLFGPFLWVIAGVGSWTFLYSVYQIDVGRLDFSF
                     VILLSIVLLLSSRISVPIPRLSSQISVSDTLVFLVLLLYGGATAILVGAIEAVVSSLR
                     FSRKAATILFNLGCAAVSLFLTSQIMELGFGDVTVLAKRPASSGFLAAVGTMALVHYA
                     TNSGLVGIGAALKTAQPIWQTWRKHYLWTSITYFAGACAAGVIAVLVQLVGFYAFVIT
                     LPIIAIVFLTYRTYLKNVETSAAQADQAEKHVKELSHYIAEQDRIREQFSQMEKLSAL
                     GELASGVAHDFNNTLAGILGRAQLLLRTDDPEKIKRGLGIIIKTAEDGAKTVKRIQDF
                     ARQRRDHDFEPISIDQILTDASEITRPRWKNCAEASNIHITVDLQIQSQAMVMGDDSE
                     LREVLVNMVFNSVDAMPEGGKLLLSTSIVDKSVVIKIADTGVGMYPEIRSRIFDPFFT
                     TKGKAGLGLGLAVSFGIIRRHGGTIEVDSEYGKGTEFRIILPIASVQDSVVPDRPLHE
                     TPTALVASNEQTQGLAITRLLVVDDEDFVRDLLKEILESEGCEVLLAGSGNEALQLFS
                     ENQFDAVFTDVGMPGMSGWELAQAIRQQNQTIPIAVITGWGEAVGSNEQKAAGVDWVI
                     AKPFTADRIIDLIKEISTQSGSHRPGFSTVAA"
     gene            complement(8101..8469)
                     /locus_tag="VFH91_10580"
     CDS             complement(8101..8469)
                     /locus_tag="VFH91_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6863489.1"
                     /translation="MAEILIVDDDPVIRDTLYELLSANYRCTAAGTGEEALRKLRQQS
                     YAVVLTDISMPGLSGLELLKRIVEEFPGTPVIMISGHSDHEQAQEMLNRGAFAYLLKP
                     FRLEVVEESVARAVESRLRE"
BASE COUNT         2116 a         2227 c         2118 g         2064 t
ORIGIN      
        1 cgctttcacg ccggcgatgt actgctggtc ctcagcggat cggacattca tttccacggc
       61 atgattggac aaatctcaga cgggatggca attgccagcg accatcgcgg atcactcctg
      121 ccggcaaccg ttcaataatc agcgaacgta tccatacggg taagcgacct tcacaccaga
      181 agttgatgac ctcacctcga ttcgatgaaa tccttttttc aggtgagttg aaaggaatgt
      241 cagggctgcc tgcctgttga gagacgtatc caattctcga ataaacggat caaaactaac
      301 gggcactcct gttccctgga aaaatgcatg gccgcccgtt tcagtcgaca gctttaataa
      361 agaactctgc gcgttactgg tcaagaggaa gttgccttgc gcagccagtg tcggtgcgta
      421 gaatccgtat atcgccacgc tacgtcgttg agcttcggta atcgcccgct gtaagtcaag
      481 actttgtgtt ggcgacgacg aatcgatgcc tcgcgagata tccagaccat ccgaaatcaa
      541 taaaatggct cggcgcccga gtggctgcgc gtcaaatcgt ttcagccctt ctatcacttc
      601 gacataagga ttatatggcc caacactcgc gaatccggcc ggaggtctta aactccgcgc
      661 tgccttttca agatcaatag tgaacttttg tttgacctgt aagctacccg tcctgagata
      721 gccgatcatc acgcgcgatc cgcgaggcaa actcctgatg aattcctgta acgcctttgt
      781 ctcgttagcg acagaaggta ccaggtcttc ctgaattagt aacgcaagcg ttattggcga
      841 gtttgtaccc atgcctctaa tagatatgat gctctgaggc tctccatcct cactgactgt
      901 cagatcaatg ttctgcaact cagattcgtg ttcggcttct ttgacattaa tggtcaaagg
      961 aattgttact ggtttgcccg gagttccgtt ggaattggag ccggccacgg attgtggctg
     1021 agcattggct ggagagttaa caattgccag aagagtcagc aaagcaaacg tctttacagc
     1081 cgggtacctt cgaagagttc tcatttgatt tctcgcctca gtcggtcgtc gacattatac
     1141 aggccagtct tggaaatgct tatccgatac ttcgattgct ggcggctcga actttcatac
     1201 gctaagattt ccacggagct aaacaaagag tgagcgaagc aataggaaaa gtgtcgagcc
     1261 gcgacgtggc tgtaaatcga atgttgaagg tcgcgccctg gctggctgtt gtggttgcga
     1321 ccttaccggc acctttggtc tttttgcttc tttttgtgac ttcaacggcg acagattcgg
     1381 cggctgtatt tctacttctc gccggaacct cgctcacatt tggtttcgcg accgggctat
     1441 tgatcgcagt tatcttgctg ctctaccggc gaaagtggct tgccagatta agagaccgtt
     1501 tagcttccga tggaatcacg gctaatgagg ttgtctggtt ccgttcggaa cttacatctg
     1561 ccgaacgtgc atctctcgca gaaattgagc gaaccaaccc tttactggcc gacgcctacc
     1621 tcgaaaccct ggccagccgg ctcacagcat ccagaatcat ctccagggcc agaaacgaga
     1681 tgctaaaggt cgaacgacgc cttaatcgcg cacgttccct cgcctcagcc gacaccgaat
     1741 cgctccaaaa ggaattattg gctgatcgtg aaaggctcgc tgaagtccgc caacgggcca
     1801 gcgaacacct gactcgagca aaagcccggc tccaggaaat cgaagccacg gccagtcgga
     1861 aactgagcgt ccttgaaact gatgtgatga tgcagcgatt gggatctgct caagatcaac
     1921 tgcctcttgt ccttgaaatt gccagactgg agcaacaagc tttgctcgag gcaaataccc
     1981 aaattaatga cactccggtt aaggcaggcg gcaattaagc actcacaaca ttgccagacg
     2041 ataaactcca ggtgctaatc gtcgcgcaaa accatcgtca accagagccc agttacccag
     2101 gcctgcttcg ggcgacctta gaccggtaac tcttgccagt tctcctcgaa cagtcattcc
     2161 agctccactc aagtacgctc gcgcaatctc ctttaccgcc agctcgcggt ccactcgttc
     2221 cttcatttga tctggaaaac gtgactcagc aatggtccaa atgtaactga aggtgggctg
     2281 ataaattacg tcgctgggaa tcaccttgag tgttttctgc agctcatcca gagctttgtt
     2341 aaagcgggcc cggtcgccga ttccacattc atctcgcaag tctctcgtag ccatctccca
     2401 ctcggacctt agcactttca gaaccaaacg ggcgtcgttt gtcagttgcg acttctctct
     2461 tcctgctggg atgccgagca aactgttgaa aaagggtaat aaacgaggcg cgacaaaggt
     2521 cgccctaccc tttaacaatt tgccgtaaaa atgcctgccg cgacgcatga cttcatcttt
     2581 aatggtccag gtcaaactgc tttcggcatc tttttgaaca ttgcgcggca tgtgagcgtc
     2641 tctacggcca caaaccgcaa cgtacaagct tggaccaggt cgacgtgaat cagtaagcgc
     2701 ctcgcaaaag cccacttcct caatgaagcg ttcagcagcg aaaacattct ccacgcgcaa
     2761 ctctggatca cgcctccaaa ggcggtcacg ataactctcg atttcttcag gtaacgtcac
     2821 gacagactcg ctcaaagtga ttccaacaat cagtcattct ggaaggatcg gtcaggttgc
     2881 tgaacgacca tggctctccc cacatttccg ctaactcgtt gcaaaacaaa gggctgaccg
     2941 ttgtttcgca gtgtggcaca aacgctgcaa aaagcagagc gcccaacccc tgcggtcggg
     3001 gacaaacttt tggttgagtt acggagacta ttggacgtga aatcgattaa caaacctcga
     3061 cgcttcctca cacttagcgt gctaaccgca ttatcgcttg ctgcgatcgt gcccggcagc
     3121 ttcctcttat ccagcgcctc tagtgcggca ggtaagaatg gcaaaaatca taaaggtgga
     3181 caccataata aggtgggctc tgaccttaga gagcagatgc acggaaagca cggtggttca
     3241 ttcgtcaatg tcattctcca gctcaactct tctcccagtg gtcaattaaa cgcccttcta
     3301 agaagcaatg gtgtgcgagt taaaggacag ttctcaaact tcaattcata cgcactcgaa
     3361 ctaccggtct cagtcgttga ttccttagct atgtttccag aagttgagtt tgtttcagtg
     3421 gactctgagg ttaagacctt aggtggacac attgctcata caaccggagc agacaatgtc
     3481 agatcaatgg ctttcgatgg gtcattggat ggcaatggag tcggaatagc gatcgtcgat
     3541 tcaggcatgt acgccgctca taccgcattc agggacgccc taacgggact gtcccgaata
     3601 gttgttagta aggatttcac tggcgaaggc agaaccgacg atccttatgg acacggaact
     3661 cacgtagctg ccgctgctgc cggaagtggc atcgtctcaa gcggtgagta cattggaatc
     3721 gcgccacgag caaatctaat caatctaaga gttctgaatt cccagggagt cggcaatgta
     3781 tcgagcgttc tatccgcact cgattggctt atggtgaacg caactagcta caacgtgcgc
     3841 gtcgtcaatc tgagtctggg aatgcctgca atcaattcga gtcaattcga tcccctctgc
     3901 cgtgcggttc gtcgcctggt agaccgagga atctttgtag tcactgccgc gggaaacaat
     3961 gggaagaata gtgacggtca gaaggtatac ggacaaatcc attctcccgg caacgaggca
     4021 tcagcactta cagtgggtgc cgtagacaca cacggaactg attcaagagg cgacgacaca
     4081 gtcgcgactt acagctcgcg tggtccgacg agaagtttct ggaccgacga attcggagtc
     4141 aagcactacg acaacatcat caagcccgaa atctcagctc caggaaacaa gaccatcttc
     4201 gcggaagctc ctgacaactt tattctttcg caaaatccct cgctcgacgc tggcgtaagc
     4261 ggtaacgtta ctcgcaagca gatgattctt agcggtacat ccatggccgc gcctctggcg
     4321 gcaggtactg cggcattatt aattgaagcc aaccccactc tcagccccaa tctcattaaa
     4381 gcgcttctga tgtacaccgc tcaacagtta ccgaattaca acatgttcga gcaaggtgcc
     4441 ggcgaactca atatcgatgg cgccgtcaga atcgccaagc tcgtccgctc ggatctgaac
     4501 gacttgaccc cgctcggatc gaacttgatt aatggttctc ttccttctca gcaaaccagc
     4561 attaccactt cgaacggtgt ctcgacgttc caatggtcca ggggaatcat ccttggacat
     4621 accttcgcga ccggtgtgga cctgatgacc aagtatcaga ggatctattc ccacggtgtt
     4681 ttgcttggtg acggtgttct tatcggtgac ggcgtcttga ttggcgatgg cgtgttgatc
     4741 ggcgacggag ttctaatcgg cgacagcaca atgctctcca acggcgtttt gataggcgac
     4801 aacatactcg ttagcaacgg caccttgatg aacagcggct cgatcttttg tgctgccggt
     4861 gtcttgattg gcgatggcgt tctaatcggc gacggagtgt tgatcggtga cggcgtcttg
     4921 attggtgatg gcgtgcttat cggtgacggc gtactcattg gtgacgctgt tattcaggcc
     4981 ctaaacgcaa tgatatcggg cgacgcgaca ccatcaatgc aaccaattcc cgacaacgaa
     5041 atcgattatt taggatttta agttaagcat tctggctcta accactcact aaggaaaaag
     5101 agagcaccaa tgttggaaac tagcggcaca gcacaactca ataaccggtc gatgggtatc
     5161 ggctcagtgg acgccggaaa tgtcacgcga ctcagaaccg gccaggaaac tgaagttctt
     5221 aactttctgt cggaacgtcc gattcatact gtagctatga tgagcatgat tcgggataac
     5281 ggcctgacga gcccatttaa ccgcggcgtc ttttacggtt accgggatct gaatggtcgc
     5341 ctggaaggcg ttgcgctaat aggtcacgct acgctgctcg agtctgtttc agaccgcgca
     5401 ctctcagctc tttccagggt ggcccgtaaa tgcggtgcta tccacatgat tatgggtgaa
     5461 caggatcgaa ttgatgaatt ctggtccagc tattccgagg aaggacacaa gaagcgccac
     5521 tcctgtatcg agcagctatt tgaattgtct acacccatgg agtttccagg cgaaactgac
     5581 agattacgat tggcgactgc tgatgaagtc gatctggtga tgccagtcca ggctgttctt
     5641 gcttttgaag agagcggcgt caatccaatg aaatcggatc ccgaaggttt tcggctgcgc
     5701 tgtttacgtc gtattcaaca aggtcgaacc tgggtggttg tggacaatgg tgctttggta
     5761 tttaaagccg aagttatctc gcagacatca ggtgtcgcct atctggaagg catctggacg
     5821 cgacacgaca aacgacaaaa cggcgattcc gtccgctttg tctctgaact ttcccggaaa
     5881 ctcttgcagg aagtcaattc tatttgcctg ctggtcaatg aaaagaatct cctcgcaaag
     5941 gcgttttatc ggaaatgcgg ttttctattt cgagcttcgt acaaaacgat tttcttgaag
     6001 acgaatgaac gctcaaacta aacattcatg ctccccggat cagtcttgat gaaaggcagc
     6061 ccttccctca ccttgagtgc aaagcagcaa aatgtcgaat accccaggca ccaaaagaaa
     6121 cccactcttc gggccattcc tctgggttat cgcaggagtt ggtagctgga cgttccttta
     6181 ctcggtctat caaatcgatg taggacgcct cgacttcagc ttcgtcatcc ttctgtcaat
     6241 cgtgctgcta ctgagctcgc gtatcagcgt cccaattcca aggctcagtt cacaaatttc
     6301 cgtttccgac acattggtct ttcttgtctt acttctttat ggcggggcaa cagcaatcct
     6361 ggttggcgcc attgaagccg tggtttcttc actcaggttc agccgcaaag cagcgacaat
     6421 actcttcaat ctaggttgcg cagccgtatc actgttcttg acaagccaaa ttatggaatt
     6481 gggattcggt gatgtaaccg tcctcgcgaa gcgacccgcc tcaagcggtt ttctggccgc
     6541 cgtcggcacc atggcccttg ttcactatgc aacaaattct ggactcgttg ggattggagc
     6601 tgcactaaag acggctcaac caatctggca aacatggcgc aaacattact tgtggacgtc
     6661 gatcacgtac ttcgccggag cttgtgccgc tggtgtaata gcggtactgg tccaactcgt
     6721 tggattttac gcttttgtaa tcacattacc gattatcgcg attgtttttc tgacctaccg
     6781 cacctatctg aaaaatgtcg aaacgtccgc ggctcaggcg gatcaggctg aaaaacatgt
     6841 taaggaactc tctcactata tagctgaaca agatcgcatc agagaacagt tctctcagat
     6901 ggagaaactg tccgcactcg gagaactagc gtccggtgta gcgcacgact tcaacaatac
     6961 gctcgccgga attctcggca gagctcaact actacttagg acagacgatc cggaaaagat
     7021 aaagcgaggg ctggggatca tcatcaagac ggcggaggat ggcgcgaaga cagttaaacg
     7081 aattcaagac tttgcccgcc agcgacgcga tcatgatttc gaacctattt caatcgatca
     7141 gattctgact gacgccagcg aaatcacgag accgcgatgg aagaactgtg ctgaagcttc
     7201 caacatccac ataactgtgg acctccagat ccagtctcag gcgatggtca tgggtgacga
     7261 ttctgagcta cgcgaagtcc ttgtcaacat ggtcttcaac tcggttgatg cgatgcccga
     7321 aggcggcaag ctcctgctat caaccagcat cgtcgacaag tcagtagtca taaagattgc
     7381 ggacacaggc gtcggcatgt atccggagat tcgttcgaga attttcgatc ctttctttac
     7441 gacgaaaggt aaagccggtt taggtcttgg cctggcggtg agctttggaa taatcagaag
     7501 acacggaggc acaatcgaag tagattccga atatggcaag ggaaccgaat tccggattat
     7561 cctgcctatc gcaagcgttc aggacagtgt ggttccggat cgacctctcc atgaaacgcc
     7621 gacggctctt gttgcttcca acgagcaaac ccagggtctt gccattacca ggttactggt
     7681 agtggacgac gaagattttg tgagagattt actgaaagag atcctcgaga gcgaaggttg
     7741 tgaagtgttg ttggcgggaa gtggaaatga agctctgcaa ctgtttagtg aaaatcagtt
     7801 tgacgctgta tttacagatg ttggcatgcc gggcatgagc ggctgggaac tcgcacaagc
     7861 gatccggcag cagaatcaaa caattccaat cgctgtaata acgggttggg gcgaagcagt
     7921 gggctcaaac gaacagaagg cagcgggagt tgattgggtc atagcaaagc cgttcactgc
     7981 cgatcgaata atcgatctca taaaggagat cagcacacaa agtgggtcgc accgtccagg
     8041 attctcgacg gtggcggctt aagtcttata ggaaaatagg accgatggga cgtataggac
     8101 ctattctcta agacgagact caacagctcg ggccacgctc tcttcgacga cctcgagcct
     8161 aaacggcttc agcaagtaag cgaaagctcc tctattcaac atttcctgcg cctgctcgtg
     8221 gtcactatga ccagagatca tgattactgg cgtccccgga aactcttcaa caatccgctt
     8281 tagcaactcc aatccgctaa gccccggcat ggagatgtcc gtaagaacta cggcgtagct
     8341 ctgttgccgc aatttgcgca gcgcctcttc tcccgtacca gctgcggtac atctataatt
     8401 cgccgacagc agttcataaa gcgtgtcgcg gatgacagga tcgtcatcga cgatcaggat
     8461 ctcagccatc atggtcctcc cacatttttt cgaccaaagg cctttcctgg gcatgcatat
     8521 tgagc
//
LOCUS       DASSFP010000470         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1920625, whole genome shotgun sequence.
ACCESSION   DASSFP010000470 DASSFP010000000
VERSION     DASSFP010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920625"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1052
                     /gene="xerD"
                     /locus_tag="VFH91_10585"
                     /pseudo
     CDS             171..1052
                     /gene="xerD"
                     /locus_tag="VFH91_10585"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
     gene            1066..1635
                     /locus_tag="VFH91_10590"
     CDS             1066..1635
                     /locus_tag="VFH91_10590"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HET6863490.1"
                     /translation="MFSLLEPSDETVRDLIARQQDVPFTYERVGATRTTPPQDFTVDH
                     NRIEIGRGEKAFKTGINAIRNWKQFDLGWVSIVPVGVKLEVGATVAVKVRVLGTWSLN
                     AARVIYLIEEPRRFGFAYGTLPDHVESGEERFSVEWLADDSVWFDILAFSRPRHPLVK
                     IYVPLARMLQKRFARNSKRRMLEEVRNSQ"
     gene            complement(1642..3213)
                     /gene="glpD"
                     /locus_tag="VFH91_10595"
     CDS             complement(1642..3213)
                     /gene="glpD"
                     /locus_tag="VFH91_10595"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HET6863491.1"
                     /translation="MDYDLIIIGAGINGAGIARDAAMRGLTVLLLDKNDLGSGTSSWS
                     TRLIHGGLRYLELGELSLVRESLRERETLLKIAPHLVRPLPILIPIQEDSRRRKTTIR
                     AGMIVYDLFSYRKSVPKHRMLSRAETLKQHPGLNPQGLLGAALYYDAQIEFPERLVLE
                     NVLSAREHGAEVLTYAKVERISSDTDRNLTVSFESRGETQQARAHIVVNASGPWVDQV
                     LEHNSFSQPRLIGGTKGSHVVVAPFDGAPTNAIYLEARSDRRPFFVIPWNNNYLIGTT
                     DVRFHGDPDSVRIESWEANYLLNETNQAFPEAKLTPDNVLYGYAGVRPLPHTKDKTEK
                     KITRRHFLRRHPQCKNLISVVGGKLTTFRSLAEECVDLVYRMMGKRSPKCLTSKVRLP
                     GAARLTSIDGAQISHNPLTEKVRMRQARIYGLFGLDLANMCLRKPELSEPISEEGDAI
                     AAEIVFAFEHELAATLTDCLMRRTMLGLNRDLGITAAEKAAKVARAHLGWTEDRLLKE
                     LSDYRAYLKRFAVGK"
BASE COUNT          823 a          881 c          807 g          825 t
ORIGIN      
        1 taaagtcggt ggtgtctgac aaagaaatta ggtgccttgg ctaggttgaa attgaagtac
       61 cacccgacta aagtcggtgg tgtccttcca actgtaatcg ccagcgtttc gctatgagct
      121 ataattccca caggttcgac atcaatcata ctgtgactca agcacgcaat ttcacaaaag
      181 aatttctcaa ctatctgcaa gtcgaaaaag ggcttgcaag gcattcgctc gacagttatc
      241 gacgcgactt aacaaagctc gagcagtggg cacaaggtaa caaacagtcg atcgttgacc
      301 tcactcgagc cgacttgcga aagtggatcg cgaatttatc gcgtgacggc cttgcacctt
      361 cttcagttgc gcgcgcggtt agcgccgccc gtggcttcta taagtttcta atgctggatg
      421 gtcatatcaa acaccacccg gctgaagatc tcgacacgcc acagcgtttt aattacctgc
      481 cgaaatttct tactgaagac gaaattagta aactgtttgc gactcccaac gtttcaactc
      541 ctgacggaat cagggacaga gcaatcctgg aagttatgta tgcatcaggt ctacgcgttt
      601 cggaaatggt gaacctgaaa caggccgatg tcgatctgct cgccgggctt ctcacctgcc
      661 acggcaaagg aagcaaggaa cggcgcgtac ctttagggaa aagcgctatt cattggttgc
      721 agcaatactc ctcggttaag gcgtcgtacg gcaagtccgg ctatccgcaa atctttttgc
      781 atagggggaa accactaaca cgacaaattg cctggttcat cattaaacag cacgccgaaa
      841 atgcaggcat tgaaaaagtt tcacctcata cactgcgcca cagcttcgcg actcaccttt
      901 tacaacatgg agctgactcg cgctctgttc aggctttgtt gggccatagc gatatctcga
      961 caacgcaaat ttacacgcac atatctgata tccacttgag atcggcctat aaccgacacc
     1021 acccgcgggc gaagttaaag ccgcacgatt gatttccagg tttaaatgtt ttcgctactt
     1081 gaaccttctg acgagacggt aagggactta atcgcccgac aacaagatgt gccttttact
     1141 tatgagcgag tcggcgctac caggaccaca ccgccgcaag acttcaccgt ggaccacaat
     1201 cgaattgaga tcggtcgagg cgagaaagct ttcaaaaccg gcatcaacgc aatacgaaat
     1261 tggaagcaat tcgatctcgg gtgggtcagc atcgttcctg tcggggtgaa gcttgaggtc
     1321 ggagcaacag tcgccgtcaa agttcgcgtg ctgggcacct ggtctcttaa tgcggctcgc
     1381 gtgatctatt tgattgaaga gcctcgcagg ttcgggtttg catacggaac cttgccggat
     1441 cacgtcgagt cgggcgaaga acgttttagc gtggaatggc tcgccgatga ctcggtctgg
     1501 ttcgacatac tggcgttttc ccgtccacga catccgctgg tgaagatcta tgttcccttg
     1561 gctcggatgc tgcaaaagcg gttcgccaga aactcaaagc gccgcatgct cgaggaagtt
     1621 cggaatagcc aatagcttac gttactttcc aactgcgaag cgctttaaat aagcgcgata
     1681 atccgaaagc tccttgagaa gtcgatcctc ggtccacccc aaatgcgctc gcgcaacttt
     1741 tgcagctttt tcagccgcgg tgattcccag atcgcgattg agtcctaaca tagttcgcct
     1801 catcaaacag tctgtcaggg tcgcagccaa ctcatgttca aaggcaaaga cgatctcggc
     1861 agcaatcgca tcaccttctt cactaattgg ttctgataat tcgggttttc gtaagcacat
     1921 gttagccaaa tcaagtccga acagaccgta gattcgcgct tgcctcattc gtactttctc
     1981 tgtcagcgga ttatgactaa tttgagcccc atcgattgat gtcagccgcg cagcaccagg
     2041 caaccttact ttagaagtca aacattttgg cgagcgctta cccatcattc tgtagaccag
     2101 atcaacgcac tcttcggcaa gacttctaaa cgtggtgagc tttcctccaa caacagaaat
     2161 cagattctta cactgcggat ggcgccgaag aaaatggcgt ctcgtaatct tcttttcggt
     2221 cttatccttg gtgtgaggca atgggcgaac gccggcataa ccgtaaagca cattgtctgg
     2281 agtgagtttc gcttcgggaa atgcttgatt ggtctcgttt aatagatagt ttgcttccca
     2341 actttcgatc cgcacgctgt ctggatcacc gtgaaaccgc acgtcagtcg taccaatcaa
     2401 atagttgttg ttccagggta tgacaaagaa aggccgtcta tcgcttcgag cctccagata
     2461 aatcgcattc gttggtgcgc cgtcaaaagg cgctactacg acgtgacttc cttttgtgcc
     2521 gccaatcaac cggggctggc taaagctgtt atgctccaga acctgatcga cccatggacc
     2581 agatgcgttg acgacgatgt gcgcgcgagc ttgttgagtt tcacctcgcg attcgaaact
     2641 cactgtcaga tttcggtccg tgtcacttga gatccgctct acctttgcat atgtcagaac
     2701 ctcggcgccg tgttcacgag cactcaacac attctccagg accaatctct cagggaattc
     2761 gatttgagcg tcgtagtaga gggcagctcc cagtaagcct tgaggattaa gccctggatg
     2821 ttgctttaga gtttcagctc tcgagagcat gcgatgcttc ggaacagact tccggtacga
     2881 gaataggtcg tagacaatca ttcctgcgcg gatcgtagtc tttcgccgtc tgctgtcttc
     2941 ttgaatcgga atgaggatcg gtaacggtcg aacaaggtga ggagcgatct tcaatagagt
     3001 ctctcgctca cgcagcgact cgcggactaa actcagctcg ccaagctcga ggtatcgcag
     3061 tccgccgtgg attaaccgag ttgaccaact actggtgccg ctaccgagat catttttatc
     3121 aagcagcaac acggttaacc ctcgcatcgc cgcgtcacga gcgatcccgg ctccgtttat
     3181 gcccgcgcca atgatgatca ggtcgtaatc catcgctgga aatttttacc tggtcgatga
     3241 cctctcatga gctatttaaa aactttgacc acgtgaagag cgcacgaaat ggtgcccggc
     3301 ttacaccttc taaggtatcc caccgtctca gtctag
//
LOCUS       DASSFP010000471         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_295459, whole genome shotgun sequence.
ACCESSION   DASSFP010000471 DASSFP010000000
VERSION     DASSFP010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295459"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFH91_10600"
     CDS             <1..449
                     /locus_tag="VFH91_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6863492.1"
                     /translation="SVALTVALATLIISALAIVLYGGTIGDTLASAYGLSDAFNTMWR
                     VLQWPIALGFVLFTFNLIYNVAPNVPAHRRRWLSPGAFVAVVLWLAISFLFRIYLHFF
                     NSYSVTYGSLGALIILMLWFYLTGVAILIGGEVNCELDPECNRGEK"
     gene            439..1206
                     /locus_tag="VFH91_10605"
     CDS             439..1206
                     /locus_tag="VFH91_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6863493.1"
                     /translation="MRNEGNDRLWVLIALLLIMCWVTEVPAQRKSKKKETKPAETKLD
                     KLRAEFVSATRDYKSSLEKLVAIYERNVTEAEEKVNVARKLFADGLVSRSQVDESERN
                     LQALKTKVSETRKQMETADTQIADVLVETQADAELARNLKAAKNGLLRSSSFIRFTGS
                     AGWGLSDAWKIQRYFSDTFHKQLPIAVFGQGAIHEKWRLDHRNAMDISLHPDSAEGQA
                     LLNFLQKNGVPFLAFRAAIPGTATGPHIHIGRPSHRY"
     gene            1238..2335
                     /locus_tag="VFH91_10610"
     CDS             1238..2335
                     /locus_tag="VFH91_10610"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HET6863494.1"
                     /translation="MIRIVLGLLLTPFFLLTADVYGAQMKEVLTEKQASHLAGLAMKC
                     ISREYPNKPEHVINSDADVKGPKTLHPAFYGCYDWHSSVHGHWMLVRLLKVFPTLPEA
                     PQIRKALNATLTVENLRAEVAYLKEPNRQSFERTYGWAWLLKLAEELHSWDDVDGDVW
                     AEAIQPLADAIVQSYKTFLPKQNYPIRTGVHPNTAFGLAFAFDYAKVVGDRELASLLD
                     ERSRTYFEKDVGYPGAWEPGGEDFFSPALMEADLMRRVMKPEQFAAWFRRFLPEVAKG
                     QPASLLQPALVTDRTDPKLVHLDGLNLSRAWAMNSVAKALPQNDPARAVLLTSAERHA
                     TAGLAQVASGDYAGEHWLASFAIYLLTTDAR"
     gene            complement(2369..>3172)
                     /locus_tag="VFH91_10615"
     CDS             complement(2369..>3172)
                     /locus_tag="VFH91_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6863495.1"
                     /translation="HELSDGNLSARVDQSLIVRRDEIGSLGKDFNLMATHIESLVSAQ
                     RRLLADISHELRSPLARQGVALGIARRRGNPEVTPALERIGREANRMNEMIGQLLDLS
                     RVESGTELIDRSRVDLASLVSDIVQDADYEARDNNRAVRFVSRDDCAVMGSFELLNSA
                     IENVVRNALRHTKPETEVEVNLRCVANNGTVQSIITVRDHGAGVPEKSLKEIFRPFYR
                     VEDARDRKSGGTGLGLAIATRAVALHKGTINARNREDGGLEVEIRIPSV"
BASE COUNT          747 a          793 c          805 g          827 t
ORIGIN      
        1 tatcagttgc gctcacggtc gcgcttgcta cgctaatcat ttctgcgctt gcgatcgttc
       61 tgtatggggg aacgattggc gacacactgg catcggccta cggattgagt gatgccttca
      121 atactatgtg gagggttctc cagtggccca tagcacttgg atttgttctg tttacgttta
      181 atctcatcta taacgtcgct ccgaatgtgc ctgcacatag acgccggtgg ctttcgcccg
      241 gagccttcgt cgccgtagta ctctggttgg caatctcttt tctcttccgc atctatcttc
      301 atttctttaa ctcctacagt gttacttacg ggtctttggg ggccttgata attctcatgc
      361 tttggttcta tctgacggga gttgctattc tgattggcgg ggaagtgaat tgcgaacttg
      421 atccggagtg caatcgaggt gagaaatgaa ggaaatgatc ggctgtgggt attaatcgcg
      481 ctgttgctga tcatgtgctg ggtgaccgaa gttcctgctc aacgcaagag caaaaagaaa
      541 gagactaaac ctgcagaaac taagctcgac aagctgagag cagaatttgt cagcgcaacc
      601 agggactaca aatcaagtct ggaaaagttg gtcgctattt atgagcgaaa tgttaccgag
      661 gctgaggaga aggtaaacgt agcaaggaaa ctgtttgcgg atggtctggt atcaagaagc
      721 caggtcgatg aaagcgaacg aaacctgcaa gcactaaaaa caaaagtctc agaaacccgc
      781 aaacagatgg agactgcgga tacccagatt gcggatgtgc ttgtcgagac ccaggcggac
      841 gctgagttag ccaggaatct taaagcagcc aaaaatggac tccttcgaag ttcgtcgttc
      901 attcgattta ccggttcagc tggctgggga ttgagcgacg catggaaaat tcaacgctat
      961 ttctccgata cgtttcataa gcagttaccg attgccgttt ttggtcaggg ggcaatccac
     1021 gaaaagtggc gtttggacca tcgaaatgct atggatattt cgcttcatcc agacagtgcc
     1081 gaaggacaag cattgttgaa ctttctgcag aagaatggag ttccgttcct cgcgtttcga
     1141 gcggcaattc cgggtaccgc aactggacca cacatacaca ttggccgtcc ttcacatcga
     1201 tattgattgg cttaaccttc agggaaaatg agtgacaatg atcagaatcg ttcttgggct
     1261 tttactgacc cctttcttcc tgctgactgc tgatgtttat ggcgcgcaaa tgaaggaagt
     1321 cttaacagag aaacaagctt ctcatcttgc tggacttgcg atgaagtgta ttagtcgcga
     1381 gtatccaaat aaacccgaac acgtcatcaa cagtgacgct gacgttaaag gcccaaagac
     1441 attgcacccg gcattctatg gttgctatga ctggcattcc tctgttcacg gccactggat
     1501 gctggtgcgt ttgttaaaag tgttccccac tctgcccgag gcgccacaaa ttcggaaagc
     1561 gctcaacgcc actcttacag ttgaaaatct tcgtgctgaa gtggcgtatc taaaggaacc
     1621 caatcgtcaa tccttcgagc gtacctatgg ttgggcgtgg ctattgaaac tagctgagga
     1681 gttgcactcc tgggacgacg ttgacggcga cgtttgggcg gaagccattc aacctttggc
     1741 tgatgcaatt gtgcagtcgt ataagacttt tctgccaaaa caaaattatc caataagaac
     1801 cggagtccat ccgaacacgg cgtttggact cgctttcgca tttgattacg cgaaggttgt
     1861 tggcgatcgt gagttagctt ccttactcga cgagcgaagt cgcacctact tcgagaagga
     1921 cgtcggctat cccggggcct gggagccggg aggcgaggac ttcttctctc cggcgctgat
     1981 ggaagccgac ctaatgcgtc gggtcatgaa gccggagcaa ttcgctgcct ggtttcgtcg
     2041 gtttctacct gaggtcgcaa aaggacaacc tgctagtctt ctacagcctg ccctggttac
     2101 agatcgcaca gatccaaaat tagttcattt ggatggcctg aacctttcgc gtgcatgggc
     2161 gatgaatagc gttgcgaaag cgcttccgca aaacgatccg gcccgagctg ttctgctaac
     2221 ttcggctgaa agacatgcga ccgccggcct ggcccaggtt gcgtcaggag attatgcggg
     2281 tgaacactgg cttgcatcgt ttgcgatcta tttgctgacg accgacgcac ggtgatcatt
     2341 tatcattgaa aaatgaatag atcctttctc acaccgaggg tatacgtatc tccacttcca
     2401 gtcctccgtc ttcacgattt ctggcgttga tggttccctt atgcaatgcg accgcgcgag
     2461 tagcaattgc cagacctaaa cctgttcctc cactttttcg atctctcgca tcctcgactc
     2521 tgtagaacgg tctgaagatc tctttcaacg atttttcagg gacgcccgct ccatgatctc
     2581 tcactgtaat tattgattga actgttccgt tgttagctac acatctgaga ttcacctcca
     2641 cttctgtttc cggtttggtg tgtcttaatg cattgcgtac aacgttttcg atcgcgctgt
     2701 ttagcagctc aaatgaaccc atcacagcgc aatcgtctcg agagacaaac ctgacagcac
     2761 gattgttgtc gcgagcctca taatcggcgt cctgaacgat gtcggagacg agactagcca
     2821 gatcgacccg agatctatca atcagttcag tgccactctc gactcttgaa agatcgagca
     2881 gctgaccgat catctcgttc attcgattgg cttcacggcc tatccgctct aacgcgggcg
     2941 tcacttcagg atttccgcgg cggcgcgcga ttcccagcgc aactccctgg cgcgcgaggg
     3001 gggagcgtaa ctcgtgcgat atgtctgcaa gcaaacgtct ttgagctgag accagcgact
     3061 caatatgagt ggccatcagg ttaaagtcct tgcccagtga tccgatctcg tctcgtctca
     3121 caatcaagga ttggtcgaca cgcgcgctca ggtttccatc tgatagttcg tg
//
LOCUS       DASSFP010000472         4767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_585935, whole genome shotgun sequence.
ACCESSION   DASSFP010000472 DASSFP010000000
VERSION     DASSFP010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585935"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFH91_10620"
     CDS             <1..592
                     /locus_tag="VFH91_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6863496.1"
                     /translation="GFTSESSFGGWSYLITRSASKGGNVLIDSPRFATQLVKRIERMN
                     GVNRMLLTHKDDIADHDRFRAHFGCERTMHAADNAGRLGIENVVDGEEPVSIDRDLLA
                     IPTPGHTRGHMVFLYQDRFLFTGDHLAWSPTRQTLTAFRSVAWYSWTEQIRSMEKLLS
                     YSFEWVLPGHGNFHHSNPAIMRADLERCVSWMRTRN"
     gene            626..1111
                     /locus_tag="VFH91_10625"
     CDS             626..1111
                     /locus_tag="VFH91_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863497.1"
                     /translation="MTRNQKIALGCGGAGCLGMIVVVVVGVLIYVFWKRERYYATRNY
                     NDNYNFNVRSNRNSNSNSDSNSNSNSDSNDNRPSSSSSLSDDEKHKLYQAAGVSGDAE
                     LVRRVSIKIGLMGEDYTPSAEYESFIKEHFSWAVRNAEFIQSINDKEKAIAYVNEHID
                     D"
     gene            1141..2940
                     /gene="lepA"
                     /locus_tag="VFH91_10630"
     CDS             1141..2940
                     /gene="lepA"
                     /locus_tag="VFH91_10630"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6863498.1"
                     /translation="MDQSRIRNFSIIAHIDHGKSTLADRLLERTGALSEREMENQVLD
                     DMDLERERGITIKAHAVRLDYKANDGVSYVLNLIDTPGHVDFSYEVSRSLSACEGALL
                     IVDASQGVEAQTLANTYLAIENNLALIPVVNKIDLPGAEPEKAIEQIEHFIGLETHNA
                     ILTSAKTGVGVGEVLEAIVRDIPPPVGDTSAPLKALIFDSWYDSYRGVIVLFRVIDGT
                     IRPGMKIRFFNTGRDYQVETIGVNRPRPTPINQLSTGEVGFMTASIKAVADVQIGDTI
                     TEAARPTTEPFPGFQEIKPMVFAGLYPVDTNQYEELRDALDKLRLNDASFFYEPESST
                     ALGFGFRCGFLGLLHMEIVQERLEREFNLDLITTAPSVRYIVTTTDGAVQEIDSPAKM
                     PDPGRLVKIEEPVIRAVILTPDEYLGGILPLLEEKRGVQKGFEYIAANRVMLTYELPL
                     NEIVLDFYDRLKSVSRGYASLDYNLAGYWESDLVKLDVLVAGEPVDALSLILHRDTAQ
                     AKGRALAEKMKQLIPRQLFEVPIQAAIGNKVIARETVKALGKNVIAKCYGGDITRKRK
                     LLEKQKEGKKRMKKVGRVEIPQEAFLAVLKVNE"
     gene            2966..4132
                     /locus_tag="VFH91_10635"
     CDS             2966..4132
                     /locus_tag="VFH91_10635"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6863499.1"
                     /translation="MPEPASQADIPVEAALLVCASRTRLDEQKIRELRKLASLTVDWN
                     CLFNLSRRHAVEQLVHKQLSRHAADIVPPEHLQKLKHLTQANFARNQIFTAELCRLIK
                     LFDASRITAIPFKGPALALFAYGDLNLRRYVDLDIMVRKEDVLAAREILLADGYESSK
                     SLNLSQQQVLLQTQHNLQFHRDQGRIIVELHWEVASHLFASSVRAEDLWSNLIDVVVN
                     DTSMKSLSANDLLFSLCIHGSRHLWQRLSWICDISELISRHEINWKVLLDRSTQTASE
                     RMFLLGLVLAEKLFGTSLPPLVKERCESDRSLKALGDTIVGRLFAGDDQAQPSTADTF
                     WYNIAVRKSWKARVRYMFYMLRPTDSDVKRHLPSTMKFFYYVERPFRLLFKPAK"
     gene            4373..>4767
                     /locus_tag="VFH91_10640"
     CDS             4373..>4767
                     /locus_tag="VFH91_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863500.1"
                     /translation="MIRTLCVILLVFSGLAAQDKSKEPAPQKAKPGYVLSVKTEPLLN
                     LSLKADKSSLPVIAADLSKRLKVPVFLGPTLKGQSVSIEFSELTLEAALQLLAPHVYV
                     DYEIETGSGNPPKALGIFLYDADQGEPPTT"
BASE COUNT         1207 a         1145 c         1223 g         1192 t
ORIGIN      
        1 tgggttcacc tctgagtcaa gttttggcgg ctggagttac ttgattacaa gaagcgcttc
       61 aaagggtggt aatgtgctta ttgattcccc gcgctttgcc acgcaactag tgaagcgaat
      121 cgagcggatg aacggcgtca accggatgct gttaacgcac aaggacgaca ttgccgatca
      181 tgatcgcttc cgcgcccact ttggctgcga acgaacaatg cacgctgccg ataacgccgg
      241 taggttgggg atagaaaatg ttgtagacgg cgaggagccc gtttcgatcg atcgggatct
      301 actagcgatt ccaacgccgg gacacacacg tggccacatg gtctttcttt atcaagatcg
      361 tttcctgttc accggagatc atctggcctg gtctccgacg cgacaaacgc tgacggcttt
      421 caggagtgtc gcctggtatt catggactga acaaatcagg tcgatggaga aattgttgtc
      481 ttacagtttt gagtgggtat tacccggaca tggaaacttt catcactcaa atcccgcaat
      541 tatgcgcgcg gatctcgaac gctgcgtaag ttggatgagg actcgtaatt aagctctttc
      601 taatcaactg gaacagggga aacgaatgac tcgaaatcaa aagattgcgc ttggttgcgg
      661 tggggccgga tgtctcggga tgatcgttgt tgtcgtagtt ggggtactga tttatgtctt
      721 ctggaaacgg gaacggtatt acgcgaccag gaactacaac gacaactata acttcaacgt
      781 acgctccaac aggaattcga actccaacag cgattcgaac tccaacagta attccgatag
      841 taacgacaat cgaccttcaa gttcgtcgag cctttctgat gacgagaagc acaaactcta
      901 ccaggccgcc ggagtaagtg gtgatgccga gttagtgcgg cgtgtctcga taaagatagg
      961 tttaatgggc gaagactata cgccaagtgc ggaatatgag tcgtttatca aagaacactt
     1021 ttcatgggcg gtccgcaacg cagagtttat ccagtcgatc aatgacaaag agaaggcgat
     1081 tgcgtacgtt aacgaacaca tcgacgatta actcttacgg tgaccagaac ttgagactaa
     1141 atggatcaat ctcgcatacg taacttttcc atcatcgcac acatcgatca cggcaaatcg
     1201 acgctcgctg accgtctcct ggaaaggacg ggagctctct ccgagcgcga gatggagaat
     1261 caggtcctcg acgacatgga tctcgaacgt gagcgaggca taaccataaa agctcatgcg
     1321 gtgaggctcg actataaggc taatgatggc gtgtcgtatg tgcttaatct gatcgatacg
     1381 ccgggccacg tcgacttctc ttatgaggta tctcgctcgc tttcggcatg cgaaggcgca
     1441 ctactgattg tcgatgcttc tcaaggtgtc gaagcccaga cgcttgctaa tacttatctg
     1501 gcgatcgaaa acaacctcgc gttgattcct gtggtcaaca agattgactt gccgggagct
     1561 gagccggaaa aggcaatcga acagattgag catttcatcg gactggaaac tcacaacgca
     1621 atccttacca gtgctaaaac cggagtcgga gttggggaag ttttagaagc catagtcagg
     1681 gatatacccc cgccagttgg cgatacaagt gccccactga aagcgctgat ctttgattcc
     1741 tggtacgact catatcgtgg cgtaattgtg cttttccgag tgattgatgg cacgattcgt
     1801 cctgggatga agatcagatt cttcaatact ggtcgcgact atcaagtaga aacaattgga
     1861 gtaaacagac cgcgaccaac cccgatcaat caattgagta ctggtgaagt aggattcatg
     1921 acggcttcga ttaaggctgt cgccgacgtg caaatcggag acaccatcac tgaagctgca
     1981 cgccccacca cagaaccatt tcctggattt caggaaatca agccgatggt ctttgctgga
     2041 ctttatccag tcgataccaa tcaatacgaa gaactgcgag atgcgctcga caagttgcgg
     2101 ctgaatgacg cgtcgttctt ttacgaacca gaatcttcca cagcgcttgg tttcggcttt
     2161 cggtgcggct ttctaggatt gctgcacatg gaaatcgtgc aggaaagatt ggagcgagag
     2221 ttcaatcttg atctgattac aactgcccca agcgttcgat atatcgttac gactacagat
     2281 ggagcagttc aagagataga ttcgccggcc aaaatgcccg acccgggtcg actcgtaaaa
     2341 attgaggagc ccgtcatccg agctgtcatt ctgactcccg atgaatatct tggtggcatc
     2401 cttccgttac tggaggaaaa gcgcggcgtg caaaagggat tcgaatacat cgccgcgaat
     2461 cgcgtgatgt tgacttacga gttgcctttg aatgaaatcg tgctcgattt ttacgatcga
     2521 ctgaagtcgg tttcccgagg ctatgcgtcg ctggattaca accttgccgg ttactgggaa
     2581 agcgatctgg taaagctgga tgttctggtc gctggtgaac ccgtcgatgc tctatcccta
     2641 atccttcacc gcgatactgc tcaggcaaag ggtcgtgctc ttgctgaaaa gatgaagcag
     2701 cttatccctc gtcaactttt tgaggtacca attcaggctg ctatcggaaa taaggtgatc
     2761 gctcgcgaaa ccgtcaaagc gttgggtaaa aacgtcatcg cgaagtgtta tggcggtgat
     2821 attacgcgga aacgaaaact gcttgagaaa cagaaggaag ggaagaagcg catgaagaag
     2881 gttggccgcg tcgagatccc ccaggaagca tttcttgccg ttttaaaagt gaacgaataa
     2941 ctggccctac gtgagcaaca tgccagtgcc tgagcccgca tcccaagccg acattccagt
     3001 tgaagccgca ttattggttt gcgcgtcgcg cacgcgctta gacgagcaaa agatacggga
     3061 gctcaggaag ctcgcgtctt taacggtgga ttggaattgt ctctttaatc tttctcgcag
     3121 acacgcagta gaacaactgg tccataagca actttcccgg cacgcggccg atattgttcc
     3181 gccggaacac ttacagaagc taaaacatct cacgcaagca aattttgcac gaaatcagat
     3241 ctttacggct gagctttgcc gtctgatcaa gttattcgat gcttcccgca ttactgccat
     3301 accattcaaa ggtcccgcgc tcgccttatt cgcctatggc gacttgaatc tgaggcgtta
     3361 tgttgatctg gacattatgg tccggaaaga agatgtgctg gcggcgcgcg agatattgct
     3421 tgcggacgga tatgagtctt caaagtcact caatctgagt cagcagcaag tcctgttgca
     3481 aacccagcac aacctgcagt tccatcgcga tcaaggtcgc ataattgtgg agttgcattg
     3541 ggaagtcgcg tcgcatctgt ttgcttcttc ggttcgagct gaagatcttt ggtcgaacct
     3601 gattgacgtt gtcgtgaatg acacgtcaat gaagtcgcta tcagctaacg atcttttgtt
     3661 ctcgctttgc attcacggct cgcgccatct gtggcagcga cttagttgga tttgcgacat
     3721 ttctgagttg atttcgaggc atgagattaa ctggaaagtc ttgttggacc gctcgacaca
     3781 aacggctagc gagcgaatgt tcctgcttgg tttggtgctg gcggagaaat tgttcggcac
     3841 aagtttgcct cccctcgtca aggaacggtg tgagtctgat agatcgttga aggctctggg
     3901 cgacacgatt gtcggtcgcc tatttgctgg tgatgatcaa gcacaaccca gtacggcaga
     3961 cacattctgg tacaacattg cagtgcgaaa gagttggaag gcacgtgtcc ggtatatgtt
     4021 ttacatgctt cggcctacag atagcgatgt aaagcgacac ttaccctcaa ccatgaaatt
     4081 tttttattac gtcgagcgcc cttttcgtct attgtttaaa ccagccaagt agaagcttgc
     4141 gagcgatgtc gtttcacgtg taatgtttga ccaaaatttc gtctcgtgct aggtttcagc
     4201 gcgtttcgaa tagagctcgc aagttaatcg tccgcgttga ggtgcgagcc gcagggtcgg
     4261 cctccagcca tttttgactg gcgactgcag tgtagttgaa ggctatcgcc attcacatac
     4321 cacactgatc tggatcacaa aacgcattca tatcgaaatt aacactagca atatgatccg
     4381 cacactttgt gtaattctac tcgtcttctc agggctcgct gctcaggata aaagcaagga
     4441 acctgctccg cagaaagcga agccaggtta cgtgctttcc gttaagactg agccactcct
     4501 gaatctcagc ctgaaggctg acaaatcaag tttgcccgtc atcgctgccg acttgtcaaa
     4561 gcgattaaaa gtaccggttt ttcttggtcc aacgttgaag gggcagtcag tttcaatcga
     4621 gtttagtgag ttaactctgg aagctgccct gcaacttctg gcgccacacg tatatgtcga
     4681 ctacgagatt gaaacaggat ctggaaatcc ccccaaagcg ctcggaattt ttctttacga
     4741 tgccgatcag ggtgagccac cgacaac
//
LOCUS       DASSFP010000473         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1779196, whole genome shotgun sequence.
ACCESSION   DASSFP010000473 DASSFP010000000
VERSION     DASSFP010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779196"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1379
                     /gene="alr"
                     /locus_tag="VFH91_10645"
     CDS             153..1379
                     /gene="alr"
                     /locus_tag="VFH91_10645"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET6863501.1"
                     /translation="MAFDSLNTRPAPGSRTTWAEINLDNLSHNYRVVQQRVGPEVKVM
                     AMLKADAYGHGAVRCAQSLSAAGVQWLGVALPEEALELRDAGIRVPILCVGGFWSGQE
                     QLCLNNDITPVVYRLDMIESLNRAAAAASLVADVHIKIDTGMGRLGIRWDHLNDFVSQ
                     LSRFKNIRIDGLMTHFAAADDASCQPLTRNQIGRFEEAVSLFRQHGYQPTHLHLANSA
                     GIFSYRESWGNMVRPGGVLYGLWRDVLSITVSGAGLRPVMSLYSTVELLKWVPPGETI
                     GYGCTFEASRNSLIASLPIGYHDGYLRGLSNRANVIVRGTFAPVVGRISMDITLVDVT
                     DVPGVALGDVVTLLGVAADHSSLEIHVEDLARIAGTLSYEVTCGISGRVPRFYIPGST
                     NEENKDEVERQTGEAG"
     gene            1351..1737
                     /locus_tag="VFH91_10650"
     CDS             1351..1737
                     /locus_tag="VFH91_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6863502.1"
                     /translation="MNDKPAKPVKTHGLSHLSLAVRDPERSLRFYQAVFGVKEYFRDA
                     HSIQVLGPGRKDVLAFEKDHILAGKGGGLTHFGFRLTKPDDIDMAVALVESAGGKILS
                     RGEFAPDSPYAFILDPDGYEIEIWYE"
     gene            1755..2462
                     /locus_tag="VFH91_10655"
     CDS             1755..2462
                     /locus_tag="VFH91_10655"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET6863503.1"
                     /translation="MATEPELRTVKANANDNVTALLYSSSKQARHGTTIILGHGAGAN
                     QLSGFMQLFAHGLSERGLDVVTFNFVYMEQKRSVPDQKAKLESCFRAVIVDAIAHKKL
                     KGNKLVIGGKSMGGRIASQVDAGLFEENDALAKEISGLVFLGYPLHPPGNPEKLRVEH
                     WQHIKAPMLFVQGTRDALGTPDEISPFLKQYKVDASIHSIEGGDHSFKAPKKFGLSQN
                     EIYETAMNEIASWTNKL"
     gene            2706..3026
                     /locus_tag="VFH91_10660"
     CDS             2706..3026
                     /locus_tag="VFH91_10660"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HET6863504.1"
                     /translation="MCPACGRSSIVSRPFHIKHHCSNCRSLFKREDGFFVGAILTNVV
                     VTELMILAAWFIGSIILGLSYEVVLPALFATGLIFPLAFYHHSWSYWLGLDYVVESLP
                     RVPN"
     gene            3459..3770
                     /locus_tag="VFH91_10665"
     CDS             3459..3770
                     /locus_tag="VFH91_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863505.1"
                     /translation="MPHTTPVKHHIWSFSTVTNKQLADNWFLRQSHRERHSINSSNGE
                     SDNPSRLSRANPEPRHELEGRIALKCIQINVPARESADQMRFSFQYHTQVSLSGNIFE
                     R"
BASE COUNT          920 a          932 c          970 g          954 t
ORIGIN      
        1 gtatcgagaa tagtttgagg atcatccaca gattgtgctg attacgccga ttctcaaaac
       61 gctgacgaat tagaaatcac catttgagct gaaaaatctg tgtaatcggc gtaatctgcg
      121 gataatcttt tgctggagaa gactggcaag acatggcatt tgactcactt aacacacgac
      181 cggctccagg ttcacgcaca acctgggcag aaatcaatct cgacaacctc tcgcacaatt
      241 accgcgtggt ccaacagcgc gttggtccag aagtaaaagt gatggcaatg ttgaaggccg
      301 atgcctatgg tcacggagct gttcgctgcg cccagagtct ttccgctgca ggtgttcaat
      361 ggttaggagt tgcgttaccc gaggaagcat tggaactacg cgacgcgggg atccgggttc
      421 caatcttatg tgtcggcgga ttctggagcg gccaggaaca actctgtctg aataacgata
      481 tcactcctgt tgtttaccgg ctcgacatga tcgagtcttt aaatcgcgct gccgccgctg
      541 ccagtctagt tgctgacgtt catattaaaa tcgatacagg gatggggcgg cttggcattc
      601 gttgggatca tttgaacgat tttgtttcgc agttaagccg ttttaagaac attcgcatcg
      661 atggtttgat gacgcacttt gcggccgctg acgacgcgag ctgtcaaccc ttaactcgta
      721 atcagattgg acgctttgaa gaagctgtca gtctgtttcg ccaacacggt taccaaccga
      781 cccaccttca tctggcaaac tctgctggaa tttttagcta tcgagaatca tggggaaaca
      841 tggtcaggcc tggcggtgtt ttgtacgggt tatggcgcga tgtactctca atcaccgtct
      901 cgggagccgg gttgcggcca gttatgtctc tctattcaac cgttgaatta ctgaagtggg
      961 tgccgcctgg agagacgata gggtatggtt gtaccttcga ggcttcccgc aacagtttga
     1021 tcgcatcttt acctattgga taccacgatg gctaccttcg agggctgtcc aaccgagcaa
     1081 atgtaattgt acgaggcact tttgctccgg tggttggcag aatatcgatg gatattacct
     1141 tagtggatgt aaccgatgtc cccggagtgg ctctcggcga tgtagtcaca ctgcttggtg
     1201 tggcggcaga tcattcttcg ttagaaattc atgttgagga tcttgcacga atagcgggta
     1261 cgctttcgta tgaagtaacc tgcggaatta gcggcagagt tccgcgattc tatatcccgg
     1321 gtagtacaaa tgaagaaaac aaggacgagg ttgaacgaca aaccggcgaa gccggttaag
     1381 acccacggac tctcgcatct gtcacttgcg gtacgtgatc cggaacgatc actcagattc
     1441 tatcaggcgg tttttggtgt caaagaatat tttcgtgatg cccatagcat tcaagttctt
     1501 ggtccaggta gaaaagatgt actggccttt gaaaaagatc atatattagc cggtaaggga
     1561 ggcggattaa ctcatttcgg gttccgtctg accaagcccg acgacattga tatggcggtc
     1621 gctctcgttg aatcagcggg tgggaaaatt ctcagccgcg gcgagttcgc tcctgactct
     1681 ccttacgcat ttattttaga tcctgacggc tatgagattg agatctggta tgaataactt
     1741 gaggggtgaa ccgcatggct acggagcctg aactaagaac tgtaaaggca aatgcaaacg
     1801 acaatgtaac agcgctttta tactcttcct caaaacaagc gcgacacggc acaacgatca
     1861 tcctgggcca cggcgccgga gcaaatcaat tgagtggctt catgcaattg tttgcacacg
     1921 gattgtccga acgcgggctg gatgtcgtca ccttcaattt cgtgtacatg gagcagaagc
     1981 ggagcgtgcc cgatcaaaag gcgaaactcg aatcctgctt tcgcgcagtt attgtcgacg
     2041 ccattgccca caagaaattg aaaggcaaca agctcgtgat tggcggtaaa tcgatgggcg
     2101 gccgcatcgc gtctcaggtt gacgccggtt tattcgaaga gaacgatgca ctggcaaaag
     2161 agatatccgg tctggttttt ctcggctatc cgcttcaccc gcccggcaat ccagagaagt
     2221 tgcgagttga gcattggcag catattaagg cgccgatgtt attcgtgcag ggaactcgtg
     2281 acgccctagg gacgcccgat gagatctcac catttctgaa gcagtacaag gtcgatgcga
     2341 gcatccactc gattgaaggt ggtgaccatt cattcaaggc ccccaagaaa tttggactct
     2401 cgcagaacga gatttacgag acagcgatga acgaaatagc ctcctggaca aataaactct
     2461 aactttggag tgcgccggcc cggcggcgct ttgtcatatc gatcctcacc aacccgcacg
     2521 catcattacg cgaacaccgt ctggcatacc cctgatcggg tgatctgtag tattttatag
     2581 accccccttg agcggaaccc ccacgctgga tacccgattc acccgacgcg aatcttatga
     2641 ttcaattaat gcaacagacc aaatttcacc atatcgtcga ggtgctcggg agaagcagca
     2701 cattaatgtg tcccgcgtgc ggccggtctt cgatagtgtc cagacctttt cacatcaaac
     2761 atcattgctc aaattgccgc tcgttgttca aacgagagga tggctttttt gttggggcaa
     2821 tactgaccaa cgtcgtcgtt accgagctga tgattttggc ggcatggttt atcggctcga
     2881 tcatactggg actgtcatat gaggtagtgc taccagcgtt gttcgcgacc gggctgatat
     2941 ttcctctcgc gttttaccat cacagctgga gttactggtt ggggttggat tatgtggtgg
     3001 aatccttgcc aagggtgccg aattaaaccg catttgacgg acacctctat cagatttcgc
     3061 gcaaactccc gatttaggtt atactgccca cagctcgcca gccggtttgt tcaggccatt
     3121 acgtccagac aacactagat gagctcacga aagcgatgaa cgcttttcgt gtatcgggcg
     3181 agttcgatat tttctttcgt ccaatggacg atccaattaa ctctcgttga gccgccgtca
     3241 tagcccaacc aggtcatgac cgacaagcgc cccggacttt attttcaaag cggcacagtg
     3301 aaagctgaaa cgggtttaga gctacaagaa agttgcagcc gtgagcttgc aacttaaggt
     3361 gctccccgta aacggttttt tctggtcgaa gcgcgtagaa agcggctagt caggtctttc
     3421 agttgataga gagaagtttc cgtcggatgc cggcttcaat gcctcatacg acgccggtca
     3481 agcaccatat ctggagcttt tccacagtta ccaacaaaca gctcgctgat aattggtttt
     3541 tacgccaatc ccaccgcgag cgacattcca ttaactccag taacggcgag tcggataacc
     3601 cgtctcgctt atcacgcgca aaccctgaac ctcgccatga gcttgaaggt cggattgcgc
     3661 tcaaatgtat tcagatcaac gtgccggccc gcgagtctgc tgaccagatg agattctctt
     3721 ttcagtatca cacgcaggtt tctctaagcg gcaacatctt tgagagataa ggttag
//
LOCUS       DASSFP010000474         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1181444, whole genome shotgun sequence.
ACCESSION   DASSFP010000474 DASSFP010000000
VERSION     DASSFP010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181444"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFH91_10670"
     CDS             <1..488
                     /locus_tag="VFH91_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6863506.1"
                     /translation="NMSRPAVAIPEKLFFKIGEVCDLAGVQAHVLRYWESEFPMLAPQ
                     KNRAGQRVYRKRDVEMALRIKELLYEDQYTIAGAKKRLTNDLRGGSKLKIVGSEDDSA
                     QPALPVEFSTEDEFVPTATFTRDPIAPRTAEERQGLKRLAAELRELLSLLEKENQNRA
                     K"
     gene            642..718
                     /locus_tag="VFH91_10675"
     tRNA            642..718
                     /locus_tag="VFH91_10675"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:676..678,aa:Pro,seq:ggg)
     gene            complement(768..1232)
                     /locus_tag="VFH91_10680"
     CDS             complement(768..1232)
                     /locus_tag="VFH91_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6863507.1"
                     /translation="MSEVVVTSKHNLQNEVRYGAGQTLITDEPADFGGEGAGPDPYTL
                     LLGALGSCISMTVMLYARRKQWPLEKVTVRLQQNQVHIKDCKECDQNSDGYVHRIERS
                     VSFEGNLAPEQISRLQEISHKCPVHRTLTSPIVIVDKQEGETLDDSPAGDFF"
     gene            1435..2595
                     /gene="sucC"
                     /locus_tag="VFH91_10685"
     CDS             1435..2595
                     /gene="sucC"
                     /locus_tag="VFH91_10685"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET6863508.1"
                     /translation="MKIHEYQGKELLKKFGVPVPRGHVARTPEEAFEAAKQLGSEVVV
                     VKAQIHAGGRGKGGGVKLAKSPEEARDLARQMLGMNLVTHQTGPEGREVRVLLIEEGL
                     PIHREFYLGIVLDRATGRPVFMASSAGGMDIEEVAAKTPEKILKETINPAVGFRSFQA
                     RKLAFGLGIPSDLINQAVKFMQSLYNAYEQLDASLMEINPFLLTKDNRLLALDAKVNF
                     DDNALFRHKDFLELRDLNEEEPLEIEASKFDLNYIKLDGNIACMVNGAGLAMATMDII
                     KLAGGEPANFLDVGGGASQERVEAAFRILLADENVKAVLINIFGGIVRCDMVARGVVE
                     AAKNLGIKIPVVVRLEGTNVEEGQRVIRDSGLNFTVAKGMKDAADKVVSLAA"
     gene            2632..>3070
                     /locus_tag="VFH91_10690"
     CDS             2632..>3070
                     /locus_tag="VFH91_10690"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HET6863509.1"
                     /translation="MSRLTTRSLLKWLARIFRFGLAAFFLFTAIAKLVVISAFTQNVG
                     ELLTASGFDPQRWSWPVTAMVIVAEIAIAILLLLRRTVRLGAIAAALLLLGFAGFAFF
                     YVYVLKGEPLECGCFGGIIASQLGVSTALRNLALIIPAIIVWLG"
BASE COUNT          723 a          716 c          834 g          797 t
ORIGIN      
        1 taaatatgag ccgtcccgct gttgccatac cggaaaaact cttcttcaag attggagagg
       61 tttgtgatct cgccggagtg caggctcatg ttcttcggta ctgggagtcg gagttcccga
      121 tgttggcccc gcagaaaaat cgtgccggcc aacgtgtcta ccggaagcga gatgtcgaga
      181 tggcactccg aatcaaggaa ctcctttacg aggaccaata cacaattgcc ggagcgaaga
      241 agcgactcac aaatgatttg cgcggcggaa gtaaactcaa aattgttggt tcagaagacg
      301 attcagctca gccggctctt ccagtggaat tttcaacgga agatgaattt gttccaaccg
      361 ccacttttac tcgcgacccg atcgctccgc gcacggctga agagagacaa ggtctgaagc
      421 gtctcgctgc cgaacttcgg gaacttctca gtttgttgga gaaggaaaat cagaatcggg
      481 ccaagtaact tggcttaaat cgctgtctag tttgttgggg cgtccctccg tgggcgccct
      541 tcgtcattat gggttatcac cgaagaattt aaatggggcg cccacggagg gacgctccta
      601 caatttgaca tttcttcact agcaggatac taagacacca acgggacgtg gcgcagcctg
      661 gtagcgcgca cgcttggggt gcgtgaggtc gcgagttcga atctcgccgt cccgaccatt
      721 ttaagggctt ccaatcgaaa ggttgaaagc ccttttaact ttccggttta gaaaaagtct
      781 cccgcgggag aatcgtcgag cgtttctcct tcttgcttgt ctacgatgac gatcggcgaa
      841 gtgagggttc tatggaccgg acacttatgc gaaatctctt gtaagcggct tatctgctcc
      901 ggagctaaat tgccttcaaa actgacagag cgttctatgc ggtggacgta gccatcagag
      961 ttctggtcgc actctttgca gtctttgata tgcacttgat tttgttgcag gcgcacggtc
     1021 accttttcaa gaggccactg ctttcgccga gcgtagagca tgacggtcat cgagatacaa
     1081 ctaccgagcg cgcccaaaag aagggtgtag ggatctggac cagctccttc gcctccaaaa
     1141 tcagcgggtt cgtcagttat aagagtctga ccggcgccat atcgcacttc gttctgtaaa
     1201 ttgtgcttag atgtaacaac tacttcgctc attaaacttc tccgtcagca tccgtttgct
     1261 ggtttcgacg tattcgaaag actgaactga tgccaatagt ttagagccgc gcagattgaa
     1321 gtacaacgca catctctcga tcggcaactg acaactgcca aaccgataag atacactcag
     1381 cgacgttcca caaaaccatt ccagtagtca tagaaagtag tttgagactg ttgcatgaaa
     1441 attcatgaat accagggcaa agaactgttg aagaaatttg gtgtacctgt gccgcgcggt
     1501 catgtagctc gcacgccgga agaagcattt gaagcggcta agcaacttgg atctgaagtt
     1561 gttgttgtga aagcccagat tcacgccgga ggtcgtggca agggtggcgg cgtaaagctt
     1621 gctaagagtc ctgaagaggc tcgggacctt gccagacaga tgttgggaat gaatttggta
     1681 acgcatcaaa ctggaccaga aggtcgcgag gttcgagtgc ttctgattga ggaaggacta
     1741 cctatccatc gagagttcta tcttggtatt gtgctggacc gcgctactgg tagacctgtg
     1801 ttcatggcat cgtcggccgg tggtatggac atagaggaag tcgctgcgaa aacgccggaa
     1861 aaaattctaa aggaaacaat caaccctgcg gttggcttca ggtctttcca ggctcgcaag
     1921 cttgcgttcg ggctcggcat tccgtctgat ttgattaatc aggcggtcaa gtttatgcaa
     1981 tctctctata acgcctacga gcagctcgat gcttcgctga tggagattaa tccgtttctg
     2041 ttgactaaag acaatcggct tcttgcgctg gacgcaaaag ttaactttga tgacaacgca
     2101 ctcttcaggc acaaggattt tctcgagctg cgagatttga atgaggaaga gccactcgag
     2161 atcgaggcct caaagtttga tttgaactac atcaagctcg atggaaatat tgcttgtatg
     2221 gtaaacggcg ctggtcttgc catggcgacg atggacatca tcaagcttgc aggcggagag
     2281 ccggcgaact ttctggatgt tggtggcgga gcttcgcagg aacgagttga agctgccttt
     2341 cgaatcttgc ttgcagatga aaacgtaaag gcggtgctga ttaatatctt tggcggtatt
     2401 gtgcgctgcg atatggtggc gcgcggtgtc gttgaagcgg cgaaaaatct cggcatcaag
     2461 atcccagtgg tggttcgact tgaaggcact aatgtggaag agggccagcg tgtaattcgc
     2521 gactctggct tgaacttcac cgttgcaaag ggaatgaagg acgctgccga taaagtagtt
     2581 tctctggcag cttaatgtgt cgacgtcggt tgtgcctgga gtaaggtgtc cttgtccaga
     2641 ttaaccacaa gaagtctctt aaaatggctg gcccggattt ttcgtttcgg gctggccgcc
     2701 tttttcttgt ttactgcaat tgccaaactt gtggttattt cggcattcac ccaaaacgtc
     2761 ggcgaactct tgaccgcatc tggttttgat ccgcaaagat ggtcctggcc agtgacagcc
     2821 atggtaatcg tcgccgagat agctatcgca attttgcttc tgctcaggcg aacagttcgc
     2881 ctaggagcaa tcgctgccgc gctattgctg ctcgggtttg cgggcttcgc tttcttttat
     2941 gtttacgtat tgaaaggcga gccgttggag tgcggctgtt tcggcggcat cattgccagt
     3001 cagttaggcg tttcaactgc gttgcgaaat cttgctctaa tcattcctgc gattattgtt
     3061 tggcttggtt
//
LOCUS       DASSFP010000475         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1337654, whole genome shotgun sequence.
ACCESSION   DASSFP010000475 DASSFP010000000
VERSION     DASSFP010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337654"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..561)
                     /locus_tag="VFH91_10695"
     CDS             complement(193..561)
                     /locus_tag="VFH91_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6863510.1"
                     /translation="MEHTEGFLKLVNAVKPNIHEISVSEAQERISKDPNVKLVDVRED
                     HEWEAEHAKGAIHLGKGIIERDIERTVPDQTTEIILYCGGGYRSALAANALQKMGYTN
                     VLSMAGGWKAWQESGAPTER"
     gene            753..2159
                     /gene="lpdA"
                     /locus_tag="VFH91_10700"
     CDS             753..2159
                     /gene="lpdA"
                     /locus_tag="VFH91_10700"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET6863511.1"
                     /translation="MANEKFDVTVIGSGPGGYVAAIKAGQLGLKVAIVEKDNRLGGTC
                     TLRGCIPTKQMLMSAHVFEQMQHAADFGVQASGIQLAFADVQKRKDKVVLKNSKGIEY
                     LMKKNKVVVFKGTGKLALPGKVEITDAEGKKQTIETSNIIIATGSVVRPIPGFETDGN
                     RVVNSDHILELKDVPKSLIVMGCGAVGVEFASVYSRFGAQTTIVELLPRIVPLEDEEV
                     SKELEKSFRKRGIKSQLDTKLEKLEKTETGVRVTGKTSKGEPVSLEAEMLLVAVGRMP
                     YTQGLGLEGTKIKVEKGFIQVDEFQQTAEKGVYAIGDVIPTPLLAHLASKEGIVAVEH
                     LAGKKDVRPINLRLVPNCTYCDPEVASVGLSEAKAKEQGYDVKVGRFPFSASGKARIL
                     GEEEGFVKIVSEKKYDEILGVHIIGPHATELIAEACVAMQLESTADELGRTMHAHPTV
                     AEAVMEAAEGVHDMAIHI"
     gene            2165..3340
                     /locus_tag="VFH91_10705"
     CDS             2165..3340
                     /locus_tag="VFH91_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET6863512.1"
                     /translation="MDQDVMDQDTNKRERKNGKDTKTRRLPPEPRFEMRINTRLAPSS
                     PADGQPLSCTAETTLKPAQLLDLYRFLKLTRLVEERLVNLYRQTKVVGGVFRSLGQEA
                     TAVGTAYALQPGDFITPLIRDLGAVLVKGIKPREIFAQYMAKAWGPSGGRDLNIHFGD
                     MDKGFIGPISHLGDMVPVMTGLLLAARMQSKKIVAVAYIGDGGMSTGAFHEGLNFAAV
                     QRLPLIVVAEHNHYAYSTPTDLQTAVKDLAEKAAGYGIPAYIVDGNDVIACYEVTKQA
                     AEDARNGGGAVLIEAKTYRRKGHAEHDDQRYVAEGEVEWWEKNYDPIDRFEKYLLDHS
                     VATREKLDEIVAAVNKEIDDDCAWAESSPMPDGEQAAFGVFDNENVKPALRPRVLES"
     gene            3345..4373
                     /locus_tag="VFH91_10710"
     CDS             3345..4373
                     /locus_tag="VFH91_10710"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET6863513.1"
                     /translation="MSTAVKKEHKSARPERGGPATLVEAIRQGIWEEMERDPTVFVIG
                     EDVGAYGGAFKVTDGLIDQFGKERVVDTPISEAAIVGAACGAALMGMKPVAEFQFIDF
                     ISAGFDLLTNYAAKCRYRWGAGISAVFRGPCGSGVRSGPFHSLNAEAFFLNTAGLKIV
                     EPSTAYDAKGLIKAAIRDPDPVLYFEHKKLYRLPRLREELPSDDYIVEIGKARTVKEG
                     RDLTIITFGAMVLTAIDAAEELEKEGLDIEIIDLRTLAPLDKEAILKSVKKTSKAMVL
                     HEASRTGGIGGELAAIIAEEAFEWLDAPIIRVASIDTPVPYSPPLEDYYLPQIKDVVE
                     AARKLAAY"
     gene            4444..>5355
                     /locus_tag="VFH91_10715"
     CDS             4444..>5355
                     /locus_tag="VFH91_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET6863514.1"
                     /translation="MSTEVVMPQMGESIAEGTITKWLKKIGERVERDEPLFEISTDKV
                     DAEIPSPAAGTLTEVRFNEGDTVEVNTVVAVLDGNGAAPAAPAAPAQETKAAAPVQEP
                     KPVAPPPPAPEPLAAPRVESKPVETVAQPAVSGPETTDLRAISVTPPTSSPSTAESTA
                     EDLRRTKSSPLVRKIAQEHGVDISRLEGTGLSGRVTKNDILSFIESGSSAQPKAAAPA
                     SAPSTPAPMPQEPPIAPPPARPVPGDRVEPMSVMRKKIAEHMTMSRRTSAHVTTVYEI
                     DMTRIAKLRDEYRESFFQRTGTKLTFMP"
BASE COUNT         1378 a         1293 c         1447 g         1237 t
ORIGIN      
        1 gccggtaatc caattgcaac cttgaatttc aacccttcct ttcaacaggt aactggcgcg
       61 gatggattct tcttccgcga acgccagatt cagttggctg tcaggtttga gttctagtct
      121 gtagttgttt acaaattaaa aaggcgcgtt gcttaaaaac aacgcgccct ttttcttaag
      181 aaggtatgtc ggttaccgct cggttggagc tccgctttct tgccaggctt tccatcctcc
      241 ggccatggaa agcacattcg tatatcccat cttctggagg gcattggcag ccagtgctga
      301 gcgatagcca ccgccgcaat agaggataat ttcggttgtt tggtccggaa cggtccgttc
      361 gatgtcccgt tcgataatcc ctttacccag atgaatcgcg cctttagcat gctccgcttc
      421 ccattcgtgg tcctctcgga cgtcaaccag ttttacgttc gggtccttgc tgatgcgttc
      481 ttgagcttca gatacggata tctcgtggat attgggtttt accgcattca ctagcttcag
      541 aaatccttca gtgtgttcca ttcttcgatg gtccttttta gttggccgtc aacaagacgt
      601 ctgatttggt ggcaagctgc caaaccatta taatgcccgg tctttcgaaa aggcgccaac
      661 agcatcagtg agcgaaggta tcgtgtgagt cgattttata ttgcccacct tttaatcgtc
      721 gctaatcacc atattttacg agagggacta aattggctaa cgaaaagttt gatgtgacag
      781 ttatcggcag cggtccgggc ggatatgtag cagctattaa ggccggacaa ttgggactca
      841 aggttgcgat cgtcgaaaag gacaatcggt taggcggaac ttgcacgctt cgcgggtgta
      901 tcccgacaaa gcagatgttg atgtctgcgc acgtcttcga acaaatgcag catgccgctg
      961 atttcggtgt gcaagcttcg ggaattcaat tggcattcgc cgatgtccag aaacggaagg
     1021 acaaagtcgt actaaaaaac tcaaagggaa ttgaatacct gatgaagaaa aataaggtcg
     1081 tggtattcaa gggaaccgga aaactggccc tacctggaaa ggttgaaatc accgatgctg
     1141 aaggtaagaa gcaaacgatc gaaacttcaa acatcataat cgcgactggc tcagtggttc
     1201 gcccgatacc aggctttgaa actgatggaa accgtgttgt gaacagcgat cacattctag
     1261 agttgaaaga tgttccaaag tcattgatcg tcatgggttg tggagcagtc ggagttgaat
     1321 ttgcttccgt ctattctcgt ttcggcgccc aaaccacaat tgtcgagttg ttgccaagaa
     1381 tcgttccgtt agaagacgaa gaagtttcga aggaactgga gaagagcttt cggaaaagag
     1441 gtatcaaatc gcaactcgat accaagcttg agaaattgga aaaaaccgaa actggtgtac
     1501 gagtcacagg caaaacttca aaaggagaac ccgtcagtct cgaggcagaa atgctgctag
     1561 ttgctgtcgg ccgaatgcct tacacccagg gccttggtct cgaaggtaca aaaataaaag
     1621 tcgaaaaggg ttttattcaa gttgacgaat tccagcagac ggccgagaaa ggcgtttacg
     1681 ctattggaga tgttatacca actccgctgt tggcccatct cgcctctaaa gaaggaatag
     1741 ttgcggttga gcatttggct ggtaaaaagg acgtcaggcc catcaatttg cgactggttc
     1801 caaactgtac gtattgtgat ccggaagttg cgtcagtggg tttgagtgaa gccaaagcca
     1861 aagagcaggg gtacgatgtc aaggtaggac gattcccttt ttcagcttca ggaaaagcgc
     1921 gcatccttgg tgaagaagaa ggattcgtga agatcgttag cgagaagaag tacgacgaga
     1981 tacttggggt acatatcatt ggaccacatg caacagaact gattgcggag gcatgtgttg
     2041 cgatgcaact tgagtcgaca gccgatgagc tcggtcgaac gatgcacgct cacccaacag
     2101 tcgccgaagc agttatggag gcggccgaag gcgtccacga tatggcgata catatctaaa
     2161 attgatggac caggatgtga tggaccagga cacaaacaaa cgcgagcgaa agaacggtaa
     2221 agatactaag acgcggcggc ttccgccaga gccgcgtttt gagatgcgga tcaatacacg
     2281 gcttgcgcca tcgagtcccg ccgatggaca acctttaagc tgcactgctg agacaactct
     2341 caagccggcc cagctacttg acctgtaccg gtttttgaag ttgacgcggt tggtcgaaga
     2401 gcggttagta aatctctacc ggcaaaccaa agttgtcgga ggcgtctttc gctctttggg
     2461 ccaggaagca accgctgtcg gcacggctta cgctctccag ccaggagatt tcatcacgcc
     2521 attgatccgt gacctgggtg cagttcttgt aaaaggtatt aaacccaggg agatcttcgc
     2581 acagtacatg gcgaaggcat ggggaccatc tggcggaaga gatctcaata ttcacttcgg
     2641 tgacatggac aagggcttta ttggtccaat ctcacatctt ggcgacatgg ttccggtgat
     2701 gaccgggtta cttcttgctg ctcgaatgca gagtaagaaa atagtcgctg ttgcttacat
     2761 cggcgatggt ggaatgagca ccggagcatt tcacgaaggg cttaactttg ccgcagtaca
     2821 gcggctcccg ttgattgtag ttgcggaaca taaccattac gcgtactcaa cgcctaccga
     2881 tttacagacc gcagttaagg accttgcgga aaaggcggca ggttacggaa ttccggcata
     2941 cattgtggat ggtaatgacg ttatagcttg ttacgaggta acgaagcagg ctgcggagga
     3001 tgcgcgcaac ggtggtggcg cagtgcttat cgaagcaaag acatatcgtc gcaaaggtca
     3061 cgcagaacac gatgatcagc gctacgtcgc cgaaggagaa gttgagtggt gggagaagaa
     3121 ctacgatcca atcgaccgct ttgagaaata tttgctcgac cactccgtcg caactcgtga
     3181 aaaactggat gagatagtcg ccgcggttaa caaagagatc gacgacgact gtgcatgggc
     3241 cgaatcatct ccaatgcctg atggtgagca agcagcattc ggagttttcg ataacgagaa
     3301 tgtgaaaccg gccctcaggc caagagtttt ggagtcctaa accgatgtcg acagctgtaa
     3361 aaaaagaaca taaatcggcg cgtcctgaac gtggaggacc ggcgacactg gttgaagcga
     3421 ttcgccaggg tatctgggaa gaaatggaac gcgatccaac cgtcttcgtg attggcgaag
     3481 atgttggcgc ttatggtggt gcgtttaagg ttacggacgg tctgatcgac cagttcggta
     3541 aggagcgcgt tgtcgacact cctatttccg aagctgcgat tgtgggggcg gcgtgtgggg
     3601 ccgccttgat ggggatgaag cccgttgctg agtttcaatt tatcgatttc atctcggccg
     3661 gctttgacct gcttacaaac tatgccgcca agtgccgtta ccgctggggc gccggaattt
     3721 cggcggtgtt tcgtggtcca tgcggctcag gagttcggtc tgggccattt cattctctca
     3781 atgcggaagc gtttttcctc aatacagccg ggttgaagat agtcgaaccg tcaaccgcct
     3841 acgatgcgaa aggtttgatc aaagcggcaa ttcgcgaccc cgatccggtc ctctattttg
     3901 aacacaaaaa actgtatcgg ctccctcgtt tacgcgaaga acttccgtcc gacgattaca
     3961 ttgtagagat cggtaaggcg cgtactgtta aagaaggtcg tgaccttacc atcatcactt
     4021 tcggagcgat ggtcctaacc gctattgatg cggctgagga acttgaaaaa gaaggcctgg
     4081 atatcgagat catcgatttg agaacgctcg cgccgctcga caaagaagcg attcttaaaa
     4141 gtgttaagaa aaccagcaag gcaatggtgc ttcatgaagc gtcgcgtaca ggtggcattg
     4201 gcggtgagtt ggcagcgata atcgccgaag aagcgtttga atggctcgac gcacccatca
     4261 tcagggtagc ctccattgat acgccggtcc cctattcgcc cccgcttgag gattattatc
     4321 ttccacaaat taaagatgtt gtcgaagctg ctcgtaagct cgcggcctac tgatcgatga
     4381 gagtgtcagt ttcgcgataa gcagctttga cttatagctg attggaacag aaggaaccag
     4441 accatgagta cagaagtggt gatgccgcag atgggcgaat ccatcgccga gggaacaatc
     4501 acaaagtggt taaaaaagat cggagagcgg gtcgagcgtg atgaaccgct ttttgaaatc
     4561 tcgaccgaca aagtcgatgc cgaaattccc tcacccgctg cgggcacgct aactgaagtg
     4621 agatttaatg aaggcgatac tgtggaggta aacacggtgg tagcggtcct tgatggcaat
     4681 ggcgccgcgc ctgccgctcc tgcagctcca gcgcaagaga ccaaagcagc cgctccagtc
     4741 caggagccca agcctgttgc gcctcctcca ccggctccag aaccgttagc agctcccagg
     4801 gttgagtcaa aaccagtcga aacagtggct caaccagctg tctctggacc cgagaccacg
     4861 gaccttcgcg caatatcggt gacgcctccg acctcatcac cgtccaccgc cgagtcgact
     4921 gccgaagatc tgcggcgaac caaatcgagt cctctggtca gaaagattgc tcaagagcac
     4981 ggcgttgata tatcacggtt ggaaggaact ggtcttagcg gtcgagttac aaaaaacgac
     5041 atcctgagct ttatcgaatc aggatcctcg gcccaaccaa aggctgccgc accagctagt
     5101 gcaccatcaa caccagctcc tatgccacaa gagccgccga tagcacctcc acctgctcgg
     5161 ccggttcccg gtgaccgcgt tgagccgatg agcgtgatgc ggaagaagat cgccgagcac
     5221 atgaccatgt cgcggcgtac ttcggcccac gtcactacag tctatgagat cgatatgact
     5281 cgaattgcga aacttcgcga cgagtatcga gagtcgttct ttcagcgcac cggaacaaaa
     5341 cttaccttca tgcct
//
LOCUS       DASSFP010000476         6419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1923437, whole genome shotgun sequence.
ACCESSION   DASSFP010000476 DASSFP010000000
VERSION     DASSFP010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923437"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /gene="lon"
                     /locus_tag="VFH91_10720"
     CDS             <1..1484
                     /gene="lon"
                     /locus_tag="VFH91_10720"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6863515.1"
                     /translation="DLIKAEEVLNEDHFGLEKIKERILEYLAVRQLVKNPRGSILCFV
                     GPPGVGKTSLGKSIARATGRKFIRLSLGGVRDEAEIRGHRRTYIGALPGQIIQMMKKA
                     GTVNPVLLLDEVDKLGADFRGDPSAALLEVLDPEQNHTFQDHYLDVEYDLSRVFFIAT
                     ANVLHTIPPALQDRLEILRLSGYTEREKLEIAKRHLVPKQADANGLKKEQLDFTDEGI
                     LEVIRHYTREAGVRNLEREIGSCCRKIARKFVSEQVGEDATDRIDAERVREMLGPIKF
                     RQQGVAAHSEIGLATGLAWTEVGGEVLQIEATLVKGRGGVTLTGKLGEVMQESAQAAL
                     TCIKARAERLGMSLDFIRKRDLHIHIPEGAIPKDGPSAGITMATAMASALARVPVKKN
                     VAMTGEITLRGRVLPIGGVKEKLLAAHRFGIDTIVLPKDNEKDLVDLPEEVVDTLTIN
                     FVDTIDDVLAIALEDACPTDAATTDGAPPLWTTEPPSQGIQTS"
     gene            1666..2115
                     /locus_tag="VFH91_10725"
     CDS             1666..2115
                     /locus_tag="VFH91_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863516.1"
                     /translation="MSKHKDADLILKLYDLRREATMREARNWFFSFNPTKVEDFMDVL
                     MSDKSGYYRMVTSYWDMAASLVNNGAIDPQMFNEANGEHLFVYAKIEPFIEDLRREFN
                     APQALAHLEKLVKQVPNYEQRLTAIRERMKEMAVRMAQRQAAAATAS"
     gene            2222..2797
                     /gene="yihA"
                     /locus_tag="VFH91_10730"
     CDS             2222..2797
                     /gene="yihA"
                     /locus_tag="VFH91_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET6863517.1"
                     /translation="MKVTSAEFLKSAFVESDWPKDRMAEIAFLGRSNVGKSSLINSLL
                     AVKGLARTSSTPGRTQSLNFFEINKQFRFVDLPGFGYARVPQALRSGWGEMVTGYLAN
                     RSQLVLSIHIVDSRHEPTKLDLQLHEWLGHNDKPRIIVATKSDKLSNNELRKNLEHIA
                     HVLNHNGVMAYSAKTARGRDELWRAIKSAIA"
     gene            3147..4529
                     /gene="rho"
                     /locus_tag="VFH91_10735"
     CDS             3147..4529
                     /gene="rho"
                     /locus_tag="VFH91_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET6863518.1"
                     /translation="MSQRTRSRASSANNDANAEDGGTVATAEVPERETREGREGKNEG
                     FHNLADLKEMSISKLTQIAKSMDVPGATGMRKQELIFKVLAAQTEKSGLIFSEGVLET
                     LPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQIRPPKEGERYFALIK
                     VEAINFESPDQAREKVFFDNLTPLYPEEQLKMEATTENLSARVIDLVTPLGKGQRGLI
                     VAPPRTGKTMLLQTIANSVTQNHPEVTLIVLLIDERPEEVTDMQRSVNGEVISSTFDE
                     PPTRHVQVADMVIEKAKRLVEHKRDVVILLDSITRLARAHNAVVPPSGKILSGGIDSN
                     ALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEEFKGPGNSEIHLDRRLS
                     DKRLFPAIDLQRSGTRKEELLISKEDLNRIWVMRRVLNPLSPVEQMEVVLERLEKTKS
                     NAEFLASMQS"
     gene            4619..6046
                     /locus_tag="VFH91_10740"
     CDS             4619..6046
                     /locus_tag="VFH91_10740"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HET6863519.1"
                     /translation="MRIAILTSEAMPFAKTGGLADVSGALPKALKELDVDARLFLPFY
                     QQIDRNLISGPAIENVHVEWRGQVHQVRFLQSYAAGAPAYLLDAPDFFARPSVYGFSD
                     DHVRFAFFSRAALTFLKHLDWAPDVVHGNDWPCGFAMAELRARRRYDPFFQGTKGLLS
                     LHNVGYQGSFDPKDLWWLGFGEPPDGNDFMLNGTASALKAGIIAADVLSTVSPRYARE
                     IQTGEQGHGLDWLLRLRSDRLAGITNGVDYDIWNPETDPHIASNFSLADLSGKTECKL
                     DLLKRFGLEADPRRPMLAIISRLVGQKGYDLIRQISRDIIDTGCFFIALGAGAREYED
                     FLQWWHNAAPRQIGIYKGYAGESLAHQIEAGADMFLMPSLYEPCGLNQMYSMRYGTVP
                     IVRATGGLDDTVEQFDARSGTGTGFKFEPYNGHALLEKIREALYFYAQPEVWTKIQQN
                     GMRIDNSWAAAARKYVELYEATLKF"
     gene            6165..>6419
                     /locus_tag="VFH91_10745"
     CDS             6165..>6419
                     /locus_tag="VFH91_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863520.1"
                     /translation="MKNLSHININRQRSLEDRLAQRLRSRKVTAAKFRAKQAQGKIRR
                     FVNTNFRKQEVIASLALRRGECNRCGACCELVFKCPFLKKN"
BASE COUNT         1624 a         1608 c         1668 g         1519 t
ORIGIN      
        1 aggacctgat caaagccgaa gaggtgctca acgaggatca tttcggactc gagaaaatta
       61 aagagcgcat acttgaatat ctcgccgttc gacagttggt taagaatcca cgcggctcga
      121 tcctctgctt cgtaggacct ccgggcgtag gaaaaaccag tcttggtaaa tcgatcgctc
      181 gagccactgg acgaaagttc attcgacttt ctcttggtgg agtacgcgac gaggcagaaa
      241 tacgcggtca ccgtcgtact tatatcggcg ctctacctgg ccagatcatt cagatgatga
      301 aaaaggccgg aactgttaat cccgttctgc tcctggatga agtggataag ttgggggcgg
      361 atttccgtgg cgacccgagt gccgcgttat tagaagttct cgatcctgaa cagaaccata
      421 ctttccagga tcactatctg gacgtcgaat acgatctgtc gagagtcttc tttatcgcga
      481 ccgctaacgt tctccacacg attccaccag cgctccagga tagactcgaa atcctgcgct
      541 tatcgggtta taccgaacgc gaaaaactcg agatagcaaa acgccatctc gtgcccaaac
      601 aagcggacgc taacggattg aaaaaggaac aactcgactt taccgacgaa ggtattctcg
      661 aggtgatccg gcattacacg cgagaagcgg gcgtacgaaa cctcgagcga gagatcggtt
      721 cgtgttgccg taagatcgcc agaaaatttg tctctgaaca agttggcgag gacgcaacag
      781 accgcatcga cgcagagcgc gtgcgagaaa tgcttggacc gatcaaattc cgtcaacagg
      841 gtgtcgcggc tcacagtgaa attggcctgg cgaccggtct ggcatggacc gaagttgggg
      901 gcgaggtctt acaaattgaa gcaacactgg ttaaaggacg cggcggagta acgcttaccg
      961 ggaaactcgg cgaggttatg caagagtccg cacaggcagc tctcacctgt attaaggcaa
     1021 gagctgaacg tctcggaatg agtctcgact ttatccgtaa gcgggatttg catatccata
     1081 ttccggaagg cgcaattcca aaggacggac cctctgctgg tattaccatg gcaaccgcca
     1141 tggcatctgc tctggctcgc gtgccggtta aaaagaatgt tgcaatgact ggcgaaatca
     1201 cacttcgcgg tcgtgtgcta cccatcggcg gcgtaaaaga gaagctcctg gcggcgcatc
     1261 gcttcggtat cgacaccatt gttcttccga aagacaacga gaaggatctg gtcgatctac
     1321 ccgaagaagt agtcgatacg cttacgatca atttcgtcga taccattgat gacgtgttgg
     1381 ccatcgcact tgaggatgct tgtcctactg acgcggcaac caccgacggg gcgcctccat
     1441 tgtggaccac cgaaccgcct tcgcaaggta tccaaaccag ctaatttctt tttctcgtac
     1501 tcgattactc aaccgattca cgctagccaa gggcggctag cgtgaattgg ttggcacatt
     1561 acttttcgtg caaagccata cggtttgtat ttaaactctc gcctgtcttt atttcgcggg
     1621 cgacttccac tctttcttcc actaattcat taggagcatt gaaaaatgag caaacacaaa
     1681 gacgctgatc tgatcttgaa gctctacgac ctgcgtcgcg aagcaactat gcgcgaagcg
     1741 cggaattggt tcttctcctt caacccgaca aaggtcgagg atttcatgga tgttttgatg
     1801 agcgacaaga gcggctacta ccgcatggtc acttcctatt gggatatggc agcctcgctt
     1861 gtaaacaatg gcgccatcga tccgcaaatg tttaacgaag cgaacgggga gcacctcttc
     1921 gtctacgcga aaattgaacc gttcatcgaa gatctccggc gcgagttcaa tgctccgcaa
     1981 gcgctggcgc atctggaaaa actcgtgaaa caggtgccca actatgaaca gcgactgact
     2041 gccatccggg aaaggatgaa ggagatggca gttcgtatgg ctcaacgcca ggcagcagca
     2101 gcgacagcaa gctaatcccg agtagttttc ccccggtgtc ttatcgagtc agggttgtcg
     2161 gaaggcaacc ctgacatcaa cttcggtttc aagcgttaac tgcagagatc ttgttttcca
     2221 gatgaaggta acgagtgcgg aatttctcaa aagcgctttc gtcgaaagcg attggccaaa
     2281 ggatagaatg gctgaaatag cttttctggg aaggtctaat gtcggcaagt caagtctgat
     2341 caactcgttg cttgcggtta agggactggc ccgcacctcc tcgacacccg gccggaccca
     2401 atctttgaat ttctttgaaa ttaataaaca gtttagattc gtggatttac ccggctttgg
     2461 gtatgctcgg gtgccacagg ccttacggtc gggctgggga gaaatggtca cgggttatct
     2521 tgccaaccga agccagcttg tgctatcaat tcatatcgta gattcgcgtc acgaacccac
     2581 aaaactggat ttgcaacttc acgagtggct tggccacaac gataagcccc gaattatagt
     2641 tgccactaaa tccgataagc tttctaacaa cgaattgaga aagaatcttg agcatattgc
     2701 acacgtgctc aatcacaacg gtgtaatggc atattccgcc aaaaccgctc gcggccgtga
     2761 tgaactttgg cgtgcaataa aaagcgcaat tgcgtgacac tcaaattagt ttcaacgttt
     2821 gcgcggtttc gatgagtaag ctagtattca gtacacacgt cggcaatccg tacaatctca
     2881 ttagcccccg aaggtcaatc ttgtttttgt gggccacgcg tgtggagcag gtggcatcca
     2941 aaaagaaatc gagatcgctg cccacgcgaa ttgaaataat tgcacaacac attgaggaga
     3001 atctttctta acacctcgcg agtgaattgg gtagcacctc gctgcccgta tctgtcgctc
     3061 taaccgcacg atcgttcact tccaacgctc aggtaattga aaagctgcaa tttatgcggc
     3121 attgaaatcg taagagaaca actgacatgt cacaacgaac acgttcacgc gcatcaagcg
     3181 cgaacaacga cgccaacgca gaagatggcg gcactgtagc aaccgccgaa gttcctgaga
     3241 gggaaaccag ggagggacgt gaaggcaaga atgaaggctt tcacaacctg gctgatctca
     3301 aagaaatgtc catatccaag ctgacgcaaa tcgcgaaaag catggatgtg ccgggcgcta
     3361 ccggcatgcg caagcaggag cttatcttca aagtattggc ggcacagacc gaaaaaagtg
     3421 gtctgatttt ttctgagggc gtactcgaaa ccttgcccga cggttttggt ttccttcgtg
     3481 cccccgaata caactatcta ccgggaccgg acgatattta cgtctcgccg tcgcaaatca
     3541 gaaaatttga cttgcgaact ggcgacaccg tgagcggcca aatacgaccg ccaaaagagg
     3601 gcgaacgtta tttcgctctg atcaaagttg aagctatcaa cttcgagtcg cccgatcagg
     3661 caagagaaaa ggttttcttc gacaacctga cgccgctcta cccagaagag cagctcaaga
     3721 tggaggcgac taccgagaac ctctcagccc gcgttatcga tctggtaaca ccgcttggca
     3781 aaggtcagcg tggacttatc gtcgcgccgc cccgcaccgg taagacgatg ttgcttcaga
     3841 caatcgctaa ttcagtgact cagaatcatc ctgaagtcac tttgattgtg ctgctgattg
     3901 atgaacgacc tgaagaagtt acggacatgc aacgctccgt caacggcgaa gttatttcat
     3961 cgacattcga cgaaccacca acacgtcacg tgcaagtcgc ggacatggtg attgagaagg
     4021 ccaaacgact cgtcgaacac aagcgagatg tagtcattct gcttgactca attacacgtc
     4081 tggctcgcgc acataacgct gtcgttccgc ctagcggcaa gattctctct ggcggtatcg
     4141 attccaatgc attgcaacga ccaaagagat tcttcggcgc tgcgcgaaac attgaagaag
     4201 gtggcagcct gacaattatc gcaactgcgc tgatcgatac cggctcccgc atggacgacg
     4261 taatcttcga agagttcaag ggacctggta actccgaaat tcatctcgac cgccgcctga
     4321 gcgataaacg attgttccct gcaatcgact tgcaacggtc tggcacgcgc aaggaagagt
     4381 tgctgatcag caaggaagac ctaaatcgaa tctgggtgat gagacgagtg ctcaatcctc
     4441 tttcacctgt cgagcaaatg gaagtcgttc tcgagcgtct ggagaaaaca aaatccaacg
     4501 ccgagttcct tgcttcgatg caaagctagt agattttcaa ttggcggagg gtgcggtgtg
     4561 attactggcc ttccgccttt tttgcgacct gcggtcgacg tctgaatttc gagctcatat
     4621 gcgtatcgcg atcctgactt ctgaagctat gcctttcgca aaaacgggag gccttgcaga
     4681 cgtttcagga gcattgccga aggctctaaa agaacttgat gtcgatgcga gactgtttct
     4741 tcctttctac cagcagattg atcgcaattt gattagtggt ccggcgatcg aaaatgtgca
     4801 cgttgaatgg cgtggccagg ttcaccaggt tcgtttctta cagagttatg ctgcgggcgc
     4861 gcccgcttac ctgttagacg ctcctgactt ttttgcgcga ccatcggttt acggcttttc
     4921 cgatgaccac gtgcggtttg catttttttc tcgtgccgcg ctgactttcc ttaaacatct
     4981 ggattgggct ccagacgttg tccatggaaa cgactggcct tgtggctttg caatggctga
     5041 attgcgagct cgccgtcgct acgatccgtt tttccaaggg acaaaaggat tgttgtcgct
     5101 tcataacgtc ggttaccaag gatcgtttga tccaaaagat ctgtggtggc tgggttttgg
     5161 tgaacctccg gacggtaacg atttcatgct caatggcact gcttcggctt tgaaggcagg
     5221 gatcatcgcg gctgacgttc tctccacggt cagtccacgt tacgcccgcg agattcaaac
     5281 aggagaacaa gggcacggtt tagactggct tttgcgtttg cgaagcgacc gtcttgccgg
     5341 aataaccaac ggagtcgact acgacatttg gaaccccgaa accgatcccc atatcgcgtc
     5401 aaacttttca ttagctgatt tgtcggggaa aaccgaatgc aaactggacc ttctgaagcg
     5461 ctttggttta gaagcggatc cgcgacgtcc aatgctggcg atcatctccc gacttgtcgg
     5521 tcagaagggt tatgacctta tccggcagat atcacgcgat attatcgaca caggatgctt
     5581 tttcattgcg cttggagccg gcgctcgaga atacgaagat tttctgcagt ggtggcacaa
     5641 cgctgcgccg cgacagatag gaatttacaa gggttacgct ggagaatctc tggctcatca
     5701 gattgaggcc ggcgctgaca tgttcttgat gccctcgtta tatgagcctt gtggtctgaa
     5761 tcagatgtac agcatgcgtt acggcacggt accgattgtc agggcgaccg gtggattaga
     5821 cgacaccgtt gagcagtttg acgcacgatc gggaaccgga accggattta aatttgagcc
     5881 ctataatggc cacgctttgc ttgaaaaaat ccgtgaagcc ctttactttt atgcccagcc
     5941 agaggtttgg acaaaaattc agcagaatgg catgcggatt gacaattcgt gggcggcagc
     6001 tgccaggaaa tacgtggaat tgtacgaggc aacgttgaag ttttaaaggt aacgggagtt
     6061 ccgaaaccgc ctgacttctt gacgactttg ttggcgcgga cccagagtcc gcgcctctcg
     6121 tatgtttggg attccgcgga ggactttttc gacgtaggtt gaagatgaag aatctgagtc
     6181 acatcaacat caacaggcag cgatcgttag aggatcgcct cgcgcaacgt ttgcgttctc
     6241 gtaaagtcac ggccgctaag tttcgtgcta aacaagcaca gggcaagatc cggagatttg
     6301 taaacacgaa tttccgcaag caggaggtga ttgcctcact cgctttgcgt cgcggggaat
     6361 gtaaccggtg tggagcatgt tgcgagctgg tcttcaagtg tccattcctg aagaagaat
//
LOCUS       DASSFP010000477         7110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1182229, whole genome shotgun sequence.
ACCESSION   DASSFP010000477 DASSFP010000000
VERSION     DASSFP010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182229"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFH91_10750"
     CDS             complement(<1..298)
                     /locus_tag="VFH91_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HET6863521.1"
                     /translation="MPKRKATLIANPKTGPYKSRRGIPIEELADYLESLGVDVSLCLT
                     SGPGDATRIAAATAGNGSADVIVAGGDGTINEAMQGIVGTKARLAIIPRGTANVL"
     gene            478..1137
                     /locus_tag="VFH91_10755"
     CDS             478..1137
                     /locus_tag="VFH91_10755"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HET6863522.1"
                     /translation="MSDNYASSTIPINTGGRQVPSQRELLILICIYLLGFAALAIKPH
                     DRADWALENLFPISQLVAVCIGYRYYKFTRLSYYLIFFYLFVQSWGGHFTYAEAAPFN
                     WLRDQFDLSRNHYDRVAHFMLGFLLAIPIKEILVQFVTASRRWMNFLTAAIVLAIGAF
                     YELIEWWVAVLATPNLGDKFLGTQGDIWDTQWDMFLALIGAVITLAIFGRRHEREMGG
                     G"
     gene            complement(1178..2644)
                     /gene="guaB"
                     /locus_tag="VFH91_10760"
     CDS             complement(1178..2644)
                     /gene="guaB"
                     /locus_tag="VFH91_10760"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET6863523.1"
                     /translation="MITTDVIEGLTFDDVLLIPARSDVLPYETDTSTQFTRNIRLQIP
                     LCSAAMDTVTEAALAIALAQQGGIGVIHKNMTVERQAEEVDKVKRSESGMIVDPVTIR
                     PDRPVHEALQVMERFHISGVPVVDEDGHLVGIITNRDLRFETRFDIPVSEIMTKQPLV
                     TVPVGTTLEQAKAVLQKHRIEKLLVVDDDKHIKGLITVKDIQKAIKYPSAAKDDLGRL
                     RVAAAIGATGDFRERADELVKARVDCLVIDTAHGHSSRVIEAVKEIKRRHTDTDLIAG
                     NVGTGDGARELIDAGVDAIKVGIGPGSICTTRVVTGAGVPQITAISGCVKAARGSGVP
                     LISDGGVKFSGDVAKAIAAGADVVMIGSLFAGTEEAPGEVILFQGRSFKSYRGMGSIG
                     AMREGSRDRYAQDLTEVESKLVPEGIEGRVPYKGTLADMVTQLVGGLRSGMGYTGCSS
                     INEFQEKTRFLRITSAGLKESHVHDVIITKEAPNYRLE"
     gene            complement(2823..3353)
                     /locus_tag="VFH91_10765"
     CDS             complement(2823..3353)
                     /locus_tag="VFH91_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6863524.1"
                     /translation="MVASIGEQLRITREGRGIQLRDICEQTRISVHYLEAIEANDYKR
                     LPGGVFNRSFIKAYARHVGYDEREAIEGYMRYLREHGESTDDVSTTPIHSKVYTETPA
                     TRSPILTVVLAVLMLALLTAAALAALHWFQKRAAAAPGRHQSAVAFIGDVAALNRDIS
                     RTSSAGLRETDRTALQ"
     gene            3863..4129
                     /locus_tag="VFH91_10770"
                     /pseudo
     CDS             3863..4129
                     /locus_tag="VFH91_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            4323..6128
                     /locus_tag="VFH91_10775"
     CDS             4323..6128
                     /locus_tag="VFH91_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6863525.1"
                     /translation="MSVDKDLVSVQQARDLVEAAHRAQSELAQFDQGKIDRICNAMAE
                     AALRDSARLGAMAVEETGYGVPDDKREKNRFAAEDVWNYFRGLRTKGIVSESKDVVEI
                     ASPRGVVAGIIPSTNPTSTAIFKILIAIKSRNAVVLSPHPSAAKCINETARVMREAGV
                     KEGLPADAVGCMTSATIEGTESLMKHKQTAVILATGGIGLVRAAYSSGKPAFGVGPGN
                     VPVCIERTADVPKAVQDILTGKCFDNGTICASEQAVVCDAPIEQAVREQFKAQGAHFL
                     SATEADQLGKVVATPQRSLNPAVVGKSVEVIAKMAGLNVPAGTRCLLADVAGVGRDYP
                     LSMEKLSPILAFYVVDGLSRCVERSREVLQYGGMGHTAGIHTRSREAAVQFGAEMPAS
                     RITVNTPTTHGAIGFSTALPPSMTLGCGSWGGNVTSDNVGPLHLMDIKRVAFETRPVK
                     SARPAVGVSSSAPTYSSVPKETPVAGGKINRDEIAAIVDRFLAGKRIEPSSAPSPLPL
                     PPIEAEAGSMYSGSTSAPQRESNGGNGNRPSVEQPVPVTRPQSSNGNKPVEFVSEDDV
                     RRAIQKGEKIYVTARTIITPAARDLGDPAEVFAKV"
     gene            6298..6627
                     /locus_tag="VFH91_10780"
     CDS             6298..6627
                     /locus_tag="VFH91_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET6863526.1"
                     /translation="MFLGKVVGTVWSTKKTPDLEGVRFLIVHPYDLDKEPTRNIVVVA
                     DRLGAGSGEMVICAYGKAARTAIGDQDMSIEAAVVGIVDRVDINDTVSDEMREAAHRL
                     ARDNGQK"
     gene            6736..>7110
                     /locus_tag="VFH91_10785"
     CDS             6736..>7110
                     /locus_tag="VFH91_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET6863527.1"
                     /translation="MANEELIYRITRAVYDRLGTTDEQTVEQLVTDIYRAVQDSVDKA
                     PRTSDSSSTSTVVPQSSAGSADRMIISVFGLDHPGIVAGVSQILAEASCSIIDINQTV
                     VQGKFAMVIIADTARAKDNASQL"
BASE COUNT         1720 a         1767 c         1873 g         1750 t
ORIGIN      
        1 ccagcacgtt agctgtaccg cgcggaataa tggctagtcg tgccttcgtg ccaacgatac
       61 cttgcatggc ttcattgatt gttccatctc ctcctgcgac aatcacgtcc gcactaccat
      121 tgccagcagt tgcggccgcg atcctggtcg catcgcctgg cccagaagtc agacacaggc
      181 taacgtcaac gcccaatgat tcgaggtaat cggcaagttc ctcgatcgga atcccgcggc
      241 gcgatttgta cggtccagtt tttggattag cgattagagt cgctttacgt ttgggcatta
      301 tagagcgcgc cggttggtac gcacgtctta aataattact ttggagtaca tcgaccattt
      361 tagaaagcgc cgacaggccg gcgcactcca aagtgcaact cggataccgg aaggcctatc
      421 ctaatttagc gattggtatg atacacccgc tgccgccggt caataactgt ctgacaaatg
      481 tccgataact acgccagttc gacaataccg attaacacgg gtggcaggca ggttccgtcc
      541 cagcgggagt tgctcatttt aatttgtatc tacctcctgg gatttgcggc gcttgcgatc
      601 aaacctcatg atcgcgcgga ctgggcgctt gaaaacttgt ttccgatatc tcaacttgtc
      661 gcagtctgta ttggttatcg atattacaag tttacgcggc tctcctatta tttgatcttc
      721 ttctatctct tcgtgcagtc gtggggaggt catttcacct atgccgaagc cgcccctttt
      781 aattggctgc gagatcaatt cgatcttagt cgcaaccatt acgatcgagt ggcccacttc
      841 atgttgggct ttctgcttgc gattccaatc aaagagatac tagtacaatt cgtcacagcc
      901 tctcgtcgct ggatgaattt cttgaccgcg gcaatcgtac tggcaattgg tgcgttctac
      961 gagctgattg agtggtgggt ggcagtattg gcgacaccga atttagggga caagttttta
     1021 gggacgcaag gcgatatttg ggatacgcag tgggacatgt tcctggctct aatcggggcg
     1081 gtaattaccc tcgccatctt tggacggcgg cacgagagag aaatgggagg gggataggtc
     1141 ctattttgtt gtataggacc tataggactg ataggaccta ttccaaccta taattcggag
     1201 cctccttggt gatgatcaca tcgtgaacat gagattcctt taatcccgct gatgtgattc
     1261 tcaagaagcg agtcttctcc tgaaactcgt tgatgctgct acacccggtg tatcccattc
     1321 cagatcgtaa accgccaaca agctgagtaa ccatgtcggc aagcgtccct ttgtatggca
     1381 ctctgccttc gatgccttcg ggtacaagtt tcgattcaac ttcagtcaaa tcttgagcat
     1441 agcgatcgcg tgaaccctct cgcattgctc ctatcgatcc cattccgcgg taagatttga
     1501 aagaccgtcc ctggaacaga atcacttcgc cgggtgcctc ttcggtacca gcgaagagcg
     1561 agccaatcat aacgacgtca gcgcccgcag ctattgcctt tgcaacatca ccggaaaact
     1621 taacgccgcc atcggagatc agtggtacac cactaccacg cgcagctttt acacaacccg
     1681 aaatcgcggt gatctgagga acaccggccc ctgttacgac acgcgtagtg cagatagatc
     1741 ccgggccaat tcccactttg attgcatcaa cgccggcatc gattaattcc ctggcaccgt
     1801 cacccgtgcc aacatttcca gcaatcaagt cggtgtcagt gtgacggcgc ttgatttcct
     1861 tcactgcctc gatgacacgc gaagaatgac cgtgtgccgt atcaatgacc aaacaatcga
     1921 ctcgcgcttt gactaattca tcggcgcgct cgcgaaaatc cccggttgcg ccgatggccg
     1981 ctgccacccg taatcggccc agatcgtctt tggcggccga tggatatttg atcgcttttt
     2041 gaatgtcctt aaccgtgatc agtcctttga tgtgtttgtc gtcgtcgaca accagaagtt
     2101 tctcgatgcg atgtttctgc aacaccgcct ttgcctgctc gagcgtggtt ccgaccggaa
     2161 cggtcacgag aggttgtttg gtcattattt cggagactgg aatgtcgaaa cgcgtttcga
     2221 accttagatc acgattcgtg atgatgccga cgaggtgtcc atcttcatca accaccggta
     2281 cgccgctgat gtggaatcgt tccatgacct ggagtgcttc gtgaactggg cggtctggtc
     2341 taatcgtcac cggatcgaca atcatgccgg actcggatct tttaacttta tcgacttctt
     2401 cagcctgtct ctcaacagtc atattcttat ggataacgcc tatgccacct tgttgggcca
     2461 atgcgattgc cagtgcggcc tctgtgacgg tgtccattgc tgcagagcaa agcgggatct
     2521 gcagacgaat gttgcgagta aattgggtcg aagtatcagt ttcgtaaggc aaaacatcgg
     2581 aacgtgcggg gatcagtaaa acatcatcaa aggtcaaacc ctctattaca tcggtggtaa
     2641 tcatgtcagt aacaataact ccttagcatt gactaggtag gcgttttcga tacgaaaaaa
     2701 aatgagatcg taatgcagga ctgcccgcct tttgagcggg cagtttagaa ctcgcatttc
     2761 tgtagcgcac acacttgaga gggttgcgcg tcgaagaccc agagcaaacc gaatggcgat
     2821 tcttactgta atgctgttct atcagtttct cgtaaccctg cgctcgacgt tctgctgatg
     2881 tcacgattca aagcagcgac atctccaata aaagcaacgg ctgattggtg ccggccagga
     2941 gcggcggcgg cgcgcttctg aaaccaatgc aatgccgcga gcgccgcggc ggttaacagt
     3001 gccaacatca gtactgccag tacgacagtt agaattggcg atctcgttgc tggagtctca
     3061 gtataaacct tggagtggat tggtgtcgtg ctgacatcat cagtcgactc tccatgctcg
     3121 cgaaggtaac gcatgtagcc ttcaatggcc tcgcgctcgt catacccgac gtggcgcgcg
     3181 taggccttta tgaagctgcg attaaagact ccgcccggca agcgcttgta atcgttcgcc
     3241 tcgattgctt ccaaataatg gactgagatg cgggtttgct cgcaaatatc gcgaagttgt
     3301 atgcctcgcc cttcgcgggt gatccgcaat tgttcaccga tactcgctac catcttccaa
     3361 tcgctcgctt ccgtcgcgtt gggcttggtt caactgagtg atgcgagctc cgttgggagt
     3421 aactgcgaaa gtgactttac ggaaccgctg tttaatctct tgagccgctg gttgcagagc
     3481 ctaaaagtct tgcgtttgcc gcaatatagc ttgcgctgct aaacgctgtc aaactttatc
     3541 acataaaggg ttttggtttg ctgcgttctc tgcgagatct cgacgagccc tgtgcgtcct
     3601 ggcggtgttc ttattaagag gcataacgaa ctatgcatac cagacgcaga caacacagag
     3661 actgccagcc atagtttgca aggctctgga atcaccacaa cgtcaatgaa ctcgttcaga
     3721 tagcgtccct tatcatcagt agtccaaaag cgaggcaggg tctcttgatt cttggtgcct
     3781 gcggtgatac ggtggcgcca acagtgaccc gattacaagt cgcttttaat ttatacaatc
     3841 ttcaaaagtg aggactaaag agatgccctt agaagctctc ggaatggttg aaaccaaagg
     3901 attcgtggga gcagtcgaag ccgcggatgc gatggtaaag gcagccaatg tgcaactgat
     3961 cggcaaagaa tatatcggcg cagggtacgt cacgatcttc gtgcgcggcg atgtaggcgc
     4021 agttaaggca gctactgatg ctggcgcggc cgcagcccga cgtgttggtg agctaataag
     4081 tgtccacgta attccgcgac cacacgcaga agttgaacga gtactaccag taaacggacg
     4141 aacaggtttg agtcccgacg aacgttcaat tcagggtggg gcacgaggac agctcggcga
     4201 aggagaagcg ggtcgctctc ttgggagtgg cgcgcgagaa gggaataagg agcgttcccg
     4261 ctaaagtcat cgccagtttg ttgcaaggaa gaggttattg aactggccat gttggaggcg
     4321 ttatgtcggt agataaagat cttgtttcag tccagcaagc tcgcgattta gtagaagccg
     4381 ctcaccgcgc tcagagtgag ttggcccagt ttgatcaggg caagatagat cgaatctgca
     4441 acgcgatggc tgaggcagct ttgcgtgatt cagcgagact tggtgcaatg gccgtcgaag
     4501 agactggcta cggcgttccc gatgacaaac gggaaaagaa caggttcgca gctgaagatg
     4561 tctggaatta ttttcgcggc ctccggacca agggcattgt ttcagaatct aaagatgtgg
     4621 tggagattgc cagtcctcgt ggagttgttg ccggtatcat cccatcgacc aatccgacat
     4681 caaccgcaat ttttaaaatc ctgatcgcaa tcaagtcgcg taacgcggtt gtcttatccc
     4741 ctcatccatc agccgcgaag tgcattaatg aaacggcccg ggttatgcgc gaagccggcg
     4801 tgaaggaagg cctaccagct gacgccgtag gttgtatgac cagtgctacg attgaaggca
     4861 ccgagagctt gatgaaacac aagcagactg ccgtcatatt agctaccggc ggtatcggtc
     4921 tcgtgagggc cgcctattcg tcaggtaaac ctgcgttcgg cgtaggacca ggaaatgtgc
     4981 cggtatgcat cgagcgtacg gccgatgtgc ccaaagcggt gcaggatatt ttgaccggta
     5041 aatgttttga caacggaaca atttgcgcgt ctgagcaggc ggtagtttgt gatgccccaa
     5101 ttgaacaagc agtgcgggaa caattcaaag ctcagggcgc acacttcttg tccgcgaccg
     5161 aagccgatca actcggaaaa gtggtggcta caccacaaag atcccttaac cctgcagttg
     5221 taggcaaatc agttgaagtg attgcaaaga tggctggtct aaatgtgcct gctggtacgc
     5281 gttgtctcct tgctgacgtt gccggagtcg gtcgagacta tccattgtcg atggagaaat
     5341 tatcgcccat cctggcgttt tatgtagtcg acggtttgag tcgttgtgtc gagcgaagca
     5401 gagaagtact gcaatatggt gggatgggcc acaccgccgg catacacaca cgatctcgtg
     5461 aggcagcagt ccagtttggg gccgagatgc cggcgtctcg cattacggtg aatacgccaa
     5521 caactcatgg ggctatcggg ttttctacag cgttaccgcc ttcaatgact ttgggttgtg
     5581 gttcgtgggg tggaaatgtc acctccgata acgttggtcc attacatttg atggatatca
     5641 agcgggttgc gttcgagact cgtccagtca agagcgcaag gccggctgtt ggagtgagtt
     5701 ccagtgctcc aacctactca tcagttccaa aggagactcc agttgccggc ggaaagatca
     5761 atcgagatga gatcgcggcg attgtcgatc ggttccttgc cggaaagaga attgagcctt
     5821 catctgctcc atcaccattg ccactgccgc caatcgaagc cgaggcgggc agtatgtata
     5881 gcggttcaac cagtgcaccg cagcgggagt cgaatggagg taacggcaat cgaccttcag
     5941 tcgaacagcc agttcccgtg acgcggccac agtcttcaaa tggcaacaag ccagttgaat
     6001 ttgtcagtga agatgatgtt cgccgagcga tacaaaaggg tgaaaagatc tacgtgacgg
     6061 ccaggaccat aattactcca gccgcgcgtg atctcggtga ccccgccgag gtgtttgcaa
     6121 aagtgtagtg ctttgcactt tgtactttgt gcttcgcact ttgtacttgg atctgagtcg
     6181 cacatttgtt gcgtgaatac ttcataatag acacgaattc aaagcacaaa gtacaaagca
     6241 ctaaattcaa agtacaaagc acaaagtaca aagtacaaag tacaaaacac caagattatg
     6301 tttttaggta aagtcgtcgg cacagtctgg tctactaaaa agacgcctga tcttgaaggc
     6361 gtcagatttt tgattgtgca tccctacgat cttgataaag agccgacacg aaatattgtc
     6421 gtggttgctg accgtctcgg tgcaggcagc ggcgagatgg tgatttgtgc atacggcaaa
     6481 gctgccagga cggcaatcgg agatcaggat atgtcgatcg aggcagccgt agttggtatt
     6541 gttgatcggg tggacattaa tgataccgtc agtgatgaaa tgcgcgaagc tgcccacagg
     6601 ctggctcgag acaatggaca aaaatgattt ttgagctttg tcctttgttc tttgtacttg
     6661 cggtgcgcta gtttttacta atttgcagca caaagcacaa agcacaaagc acaaagcaaa
     6721 gcacaaagca cattgatggc caacgaagaa ctgatctatc ggataacacg ggcggtttac
     6781 gatcgacttg gaactaccga cgagcaaaca gttgagcagt tggtcacgga catctatcgt
     6841 gcagttcaag actcagtaga taaggcacca cggacttccg actcttcgag cacttcaaca
     6901 gtagtacccc aatcttccgc gggatcagcg gatcgcatga tcatttctgt ttttggttta
     6961 gatcatcccg gcatcgtcgc cggagtttcg caaatccttg ctgaagcgag ttgcagcata
     7021 atcgatataa atcaaacggt tgtgcagggt aaatttgcca tggtaatcat agccgacaca
     7081 gctcgcgcga aagataatgc ctcacaactg
//
LOCUS       DASSFP010000478         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1924444, whole genome shotgun sequence.
ACCESSION   DASSFP010000478 DASSFP010000000
VERSION     DASSFP010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924444"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VFH91_10790"
     CDS             complement(<1..1359)
                     /locus_tag="VFH91_10790"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HET6863528.1"
                     /translation="MRLIKSSVLSATLLSLLLSSFLTITGQDARELQDEKTTSSKVKG
                     EHPLVALMRSRKSALRPELAGIHPRVYVTDKEIEDLRQRSRTTHRELWQQVLANVRAL
                     RSEPPAPPAQKRREQNDVGIAIAEAAFVYKVERDPKYLAAAKKFMDAAVSYDIWGYAN
                     NKPNVDLAAGHLLYGLGWGYDMLYNDLTEAERVRYRDKLIKQARIMAEYFKPKSGRTY
                     AYSQNHTFIPIAGLGVAAYALYGETPEAAGWASLARAIFDRVLATYSQDGYYYEGFEY
                     WIFSTPWLVHYLDAHLHSTGEDLYDQPGFRAMPQYVAHSMLPSGGYVFDFGDIFEGPL
                     TRAGEGDEYSRTHPGGRFNTNYNLLYRLAQRFNSGETQGIAAWLKGFNQVNAEDYWSL
                     VWYEPNVKAVPIEKQPASYYFKDHDVVYWRSSWSKDATAVAFKCGPSEGHHTRQLLAD
                     FPD"
     gene            complement(1387..2154)
                     /locus_tag="VFH91_10795"
     CDS             complement(1387..2154)
                     /locus_tag="VFH91_10795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET6863529.1"
                     /translation="MNNYMTLEGKVAIVTGGASGIGHATAVALAECGAAVAINYHRNE
                     TGAELLREQINANGGRAIVIQADVTKASEIEDLVRRTTAEIGPVDVLVNNAGSLIERL
                     KILDITEQRWDQVIDLNLKSAFLCSKAVIPSMVERKAGAIINLSSIAGRNGGALGSMH
                     YATAKGGLITFTKGLAKELAPYNIRVNAVSPGVIDTPYHEQFSTPEMMKNYVNAIPLG
                     RVGKADEVAKVIAFLASDAASYIAGETIEINGGMYMD"
     gene            complement(2163..>3353)
                     /locus_tag="VFH91_10800"
     CDS             complement(2163..>3353)
                     /locus_tag="VFH91_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6863530.1"
                     /translation="IDRLTISVLAPIITKDLGLTNTQYGGVVTWFLLAYTISQGLSGK
                     LYDSIGTRLGFVVSIVVWSCAAVAHAFARGIGSLSLFRFILGLGEAGNWPGAAKTVAE
                     WFPVRQRAMGMAIFNSGAAIGSIIAPPLIVLLQRKHGWQTTFIVTGVLGFGWLILWLS
                     LYDSPERHKWITQEELNLIRSDNGGTKEPTPSSSIRWVELLRYRQVWAIVLSRFLTDP
                     VWWLYITWLPKYLADARGFSLTEIGLFAWVPYVAADAGSLSGGWLSGHLISRGWSVDR
                     ARKTVILFAALLMPAGILAAFVSDPLFALALIGVVLFGFQVWINNVQTLPGDFFPDTS
                     VASVAGLGGTGAGIGSMIFILTTGWVVDHFSYVPILVIAGVLAPLGTLVLFTLSGKIE
                     RLKQ"
BASE COUNT          849 a          920 c          816 g          768 t
ORIGIN      
        1 atcgggaaaa tctgcgagca actgacgagt gtgatgtcct tcgcttggac cacacttgaa
       61 cgctactgcc gtagcatctt tggaccaact ggaacgccag tacacaacat cgtggtcttt
      121 gaagtaatac gaggccggct gcttttcaat tggcactgct ttcacatttg gttcatacca
      181 aaccagcgac cagtagtcct cggcattaac ttgattgaat ccttttaacc aggcggcgat
      241 gccctgagtc tcgccgctgt taaaacgttg ggccagacgg tagagcagat tgtaattcgt
      301 attgaagcga ccgcctggat gggtgcgcga atattcgtca ccttcgccgg cgcgcgtcaa
      361 tggaccttca aaaatgtcgc cgaaatcgaa gacatagcca ccgctgggca acatcgaatg
      421 agcgacatac tgaggcatgg cccgaaaacc aggttggtcg tagagatcct cacccgttga
      481 atgcagatgt gcatcgagat aatggaccag ccagggggtc gaaaagatcc aatactcaaa
      541 accttcgtag taatagccat cctgagagta agtagcgagt actcgatcga atatggcgcg
      601 agcgagactg gcccagccgg ccgcttcagg agtctcgcca taaagggcgt aagccgcaac
      661 ccctagtccg gctatcggga tgaacgtgtg gttctgactg tacgcgtaag tgcggccgga
      721 ttttggtttg aaatattccg ccataatccg cgcttgcttt ataagcttgt cgcgataacg
      781 tacgcgctca gcttcggtga gatcgttgta gagcatgtcg taaccccacc cgagtccata
      841 caacaagtga cctgcagcaa gatcgacatt gggcttgtta ttggcgtatc cccaaatgtc
      901 atagctgacg gcggcatcca taaacttttt cgccgcggcg agatacttcg gatcgcgctc
      961 gactttgtag acgaacgccg cttcggcaat cgcgataccg acatcattct gctccctacg
     1021 cttttgagcg ggaggagccg gcggctccga tcgtaacgct cgaacattcg cgagcacttg
     1081 ttgccacaac tcacgatgag tagttcgcga tcgctgccgc aggtcctcga tctctttgtc
     1141 agtgacataa acgcgcggat gaatacctgc taactccggc cgcagcgctg acttgcgaga
     1201 tcgcatgagc gctaccaaag gatgttcacc cttaactttg ctactggtgg ttttttcgtc
     1261 ctgaagttct cgagcgtcct gaccggtaat tgttaagaac gacgagagca ggagcgacaa
     1321 cagagtggcg gatagaactg aagatttgat tagtctcatt taagggatct tttaattatc
     1381 caatgctcag tccatgtaca tcccaccgtt gatttcaatc gtttcaccag cgatgtaact
     1441 ggccgcatcc gaggctagaa atgcaatgac ttttgcgacc tcatctgctt tcccaacgcg
     1501 tccaagcgga attgcgttga catagttctt catcatttcg ggtgtcgaaa actgttcgtg
     1561 atacggcgtg tcgataactc ccggcgaaac ggcattcacg cgaatgttat acggtgcaag
     1621 ctctttcgcc agccctttag tgaaagtgat caagccgccc tttgctgttg cgtaatgcat
     1681 cgatcccaac gctcctccat tacgccctgc tatcgacgat agattgatga tggctcccgc
     1741 ctttcgctca accatcgacg ggataaccgc cttactgcac agaaatgcgc ttttcagatt
     1801 gaggtctatg acctggtccc aacgttgctc agtgatatct agaatcttga gccgctcgat
     1861 taacgagccg gcgttattca cgagaacatc caccgggcca atttcggctg ttgttcggcg
     1921 cactagatct tcaatttcgc ttgcctttgt cacatcagct tgaataacga ttgcacgtcc
     1981 tccgttcgca ttgatctgct cgcgtaagag ctcagcgccg gtttcgttgc ggtgatagtt
     2041 gattgccact gccgcgccac attcggcaag agcgactgct gtcgcatgac cgatgccact
     2101 tgcgcctcca gtgacaattg ccactttgcc ttctaacgtc atgtagttat tcataattcc
     2161 cctcactgtt taagccgttc gatttttcca cttaaagtaa ataagacaag agtaccgaga
     2221 ggagccaaaa ctcctgcgat caccaaaatc ggtacataag agaaatgatc cacaacccaa
     2281 ccagtagtta agatgaagat catggatccg attccggctc cggtgccgcc cagacctgcc
     2341 actgaagcaa ctgaggtatc cggaaagaag tctccaggca gagtctgaac gttattgatc
     2401 cacacttgaa atccaaatag gaccacccca atcaacgcca gcgcaaagag cggatccgaa
     2461 acaaaagcgg ccagaattcc agccggcatc agcaaggctg caaacaggat gacagttttg
     2521 cgcgctctgt ccacgctcca accacggctg atgagatgac ccgataacca accaccactc
     2581 aaactgccgg catcggcagc aacgtacggg acccaggcaa ataggccgat ctcagtgaga
     2641 ctaaaaccac gtgcatcagc caggtatttt ggcaaccatg ttatgtaaag ccaccagacc
     2701 gggtcggtca gaaaacgaga gagcacaatg gcccagacct gacgatagcg aagtaactcg
     2761 acccacctta tcgaagaaga aggtgtgggc tctttggtcc cgccattgtc gcttcgaatt
     2821 aaattcagtt cttcctgggt gatccacttg tgtcgttcgg gcgaatcgta cagagacaac
     2881 caaagaatta accagccgaa cccgagaacg ccagtgacaa tgaaagtcgt ctgccagcca
     2941 tgtttcctct gcaacaacac gatcaaaggc ggcgctatga tcgaaccaat tgcagcacca
     3001 ctattgaaga tggccattcc catcgcgcgt tgtcgaactg gaaaccactc tgcgacagtt
     3061 tttgccgcgc ctggccaatt accggcttct cccaatccga gtatgaaacg aaacaaactc
     3121 aggctgccga tgcctcgcgc gaatgcatgg gccaccgcag cgcaggacca aaccacaatt
     3181 gagaccacga agcccagtcg ggtgccgata gaatcataga gtttgcccga cagtccctga
     3241 ctgatcgtgt aggccagcag aaaccaggta accactccgc cgtattgcgt attagttagt
     3301 ccgagatctt tcgtaatgat cggcgcgagc accgaaatcg tcaaacggtc tat
//
LOCUS       DASSFP010000479         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1472315, whole genome shotgun sequence.
ACCESSION   DASSFP010000479 DASSFP010000000
VERSION     DASSFP010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472315"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /gene="cyaB"
                     /locus_tag="VFH91_10805"
     CDS             complement(<1..464)
                     /gene="cyaB"
                     /locus_tag="VFH91_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HET6863531.1"
                     /translation="MPIEIEKKYRLTQDQKKIIISRLRDLEARRLKKEFEENILYTGL
                     NLDIRSTVVRLRKVGKRTILTYKKRFISSSAIKRQLEEETEVSNGDAIQNILKHLGLI
                     PSLVYEKRRETWRIGKAELVIDELPFGLFMEIEGPAAEIRKIEKALNLTGVRA"
     gene            complement(485..1813)
                     /locus_tag="VFH91_10810"
     CDS             complement(485..1813)
                     /locus_tag="VFH91_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6863532.1"
                     /translation="MKKLVAVVKREYLQRVRSRMFILTTILLPLTMALFGIVPALVLS
                     INAGGASRIAVVDQTGKLFGRLQRVLAEDEGPETATGVPNPMRNVPGSTPRTNTANDV
                     ILNNVTDLSRPIDDLKAELDQQLGAKEIDAYLLIPADIMEDGDARFFRGNSGDFFISR
                     RLRGALTRAVREQRLLDAHVDPSTVRELDRPVGLVATRVSTSGEVRDSPAGLAFVFGI
                     GLIMYISVLLYGQVVLGAVIEEKETRIAEVLFSSVKPFTLMIGKLVGVSFVALTQLAI
                     WGIAIAALGAYGISMLASRGMPTSIPRLPFVFYIYFVLFFLLGYYVYSTLYALVGSIV
                     TTAQEGGQLAMPIILLLAVGFYLFLPVSRNPDSNFAFWVSLVPFFAPITMLMRIITQT
                     PPFWQIALSLLIGFSTIVLFTWLASKIYRIGMLMYGKRASIPEIVRWLRE"
     gene            complement(1810..>2750)
                     /locus_tag="VFH91_10815"
     CDS             complement(1810..>2750)
                     /locus_tag="VFH91_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6863533.1"
                     /translation="YCLSRVRLMVENQITVQLDRVSKRFGDFTAVHDLSLEVRAGRVF
                     GLLGPNGAGKSTTIRMIVNITAPDSGTIRLCGHRIDPQLQDRIGYLPEERGLYKRMKV
                     VDQLRFFAELKNVPRATLEKRIDEWLERLKLVEWKHKRTRELSKGMQQKIQFISAVIH
                     DPDLLILDEPFSGLDPVNVSLLKEVVSDLKRAGKTIIFSTHLMETAEKLCDDICLLNR
                     SKKVLEGSLRQIKSSFGRNTVALRVEGGNGLLTQSNLISKVEHHSDETEVLLNENADP
                     QELLKQLIDSGAVVSKFEMIEPSLHNIFIEKVSEAQ"
BASE COUNT          705 a          722 c          619 g          704 t
ORIGIN      
        1 gcgcgtacac cggtcaggtt taatgccttt tcaatctttc ggatctcagc ggctgggcct
       61 tcgatttcca tgaagagtcc aaaagggagt tcgtctatga ccagttcagc ttttccgatt
      121 cgccatgtct cacgtcgctt ctcatagacg agtgagggga tgagaccaag atgcttgagg
      181 atattctgga tcgcgtcgcc atttgaaact tcagtctcct cttcgagttg tcgtttaatg
      241 gcggatgagg aaataaacct ctttttgtaa gtgaggatcg ttcgtttccc gacttttcta
      301 agacggacta ccgtggaacg tatgtcgaga ttcagtcctg tatagaggat attttcttcg
      361 aactcctttt taaggcgtcg cgcttccagg tcccgcagcc gagatatgat tatctttttc
      421 tgatcttgag tgagtcggta ctttttctcg atttcgattg gcatttcgat cagactgata
      481 ggacctattc tctcaaccat cttacaattt caggaatcga cgctctcttc ccgtacatca
      541 acatcccaat gcgataaatt ttcgacgcca gccatgtgaa taaaacgatc gtcgaaaatc
      601 cgatcagcaa cgataacgct atctgccaga atggtggagt ctgcgtgatg atccgcatca
      661 acatcgttat gggcgcgaaa aatgggacca gcgataccca gaacgcaaaa ttcgaatccg
      721 gatttcgact taccggcaag aaaagataaa agcccaccgc taacaacaaa atgatcggca
      781 tcgccaactg accaccttct tgcgcagttg ttactatcga gccaaccaac gcgtaaagcg
      841 tcgaatacac gtagtaacca agcaagaaga acaaaacaaa atagatataa aacacaaaag
      901 gcagtcgagg aatgctcgtc ggcatccctc tcgaggcaag catagagatt ccgtatgcac
      961 caagagcagc aatcgcgatt ccccagatcg ccagttgagt cagcgcaacg aatgacacgc
     1021 cgaccaactt gccgatcatc agggtgaagg gtttgactga cgaaaaaaga acctcagcta
     1081 tccgagtctc cttctcttcg atcacagcac ctaacacaac ctggccgtat agcaggaccg
     1141 agatgtacat gatcaaacct atcccgaaca caaacgccag accggctggc gaatcgcgca
     1201 cttcacctga agtactaact ctcgtagcca ccagaccaac tggtctatcg agctccctga
     1261 cagtgctcgg gtcgacatgc gcatcgagta ggcgctgctc gcgaaccgct cttgttaaag
     1321 caccccgaag tcgtcgggaa atgaagaaat cgccagagtt tcccctgaag aatctcgcat
     1381 cgccgtcttc cattatgtcc gctggaatca agaggtaagc gtcgatctcc tttgcgccta
     1441 actgctggtc cagctcggct tttagatcgt caatcggtcg ggagaggtcg gttacgttat
     1501 tcagaataac gtcattggcg gtattagtcc tgggtgtgct gcctggcaca ttcctcatcg
     1561 gattgggcac acctgtggca gtttcaggtc cctcgtcctc agcaaggaca cgctgaagtc
     1621 tgccaaacaa tttgccggtt tgatctacaa ctgcaattcg cgacgctccg cccgcattaa
     1681 tcgataacac cagagccggc acaattccaa acaacgccat cgtcaacggc aaaagaatag
     1741 tcgtgaggat aaacatacga ctacggacgc gttgtagata ttcacgtttg acgacggcca
     1801 caagcttctt cattgggcct cgctaacttt ttcgatgaag atgttgtgca aactaggctc
     1861 gatcatctca aactttgata ccacagctcc tgagtcgatc agttgcttca acaattcctg
     1921 tgggtcggcg ttttcgttta gcaagacttc cgtttcatcg ctgtggtgtt cgaccttact
     1981 tattaagttc gactgagtta gtagaccatt tcccccttcg actcttagcg cgaccgtgtt
     2041 cctaccaaaa ctactcttta tctgccggag actaccttcc agaactttct tagatctatt
     2101 caacaaacaa atgtcgtcgc agagtttttc tgcagtctcc atcaagtgcg ttgaaaagat
     2161 gatcgtcttc cctgctcttt tgagatccga cacgacctct ttaagtaacg atacgttcac
     2221 gggatcgaga ccggaaaatg gttcgtccaa aataagcaga tccgggtcat gtatcaccgc
     2281 actgatgaac tgaattttct gctgcatacc cttcgagagt tctctggtcc gcttgtgttt
     2341 ccactcgact agcttcaaac gctcgagcca ctcgtcaatt cgcttttcaa gggtggctcg
     2401 cggaacgttt ttcagctcag caaaaaatcg taattggtcg acaaccttca tccgtttgta
     2461 gaggccgcgt tcttccggaa gataaccaat ccggtcctgt agttgcggat caattcgatg
     2521 accacagagg cggattgtgc cggagtcagg agcagtgata ttgactatca ttcggatggt
     2581 agtacttttg ccagctccat ttggtccgag aagaccaaac acgcgaccgg cacgaacttc
     2641 caggctgaga tcgtgaacgg ctgtgaagtc gccaaatctt ttactgaccc ggtctagttg
     2701 tacagttatt tggttttcta ccatcaagcg aaccctcgac agacaatacc
//
LOCUS       DASSFP010000480         4523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1472339, whole genome shotgun sequence.
ACCESSION   DASSFP010000480 DASSFP010000000
VERSION     DASSFP010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472339"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..2156)
                     /locus_tag="VFH91_10820"
     CDS             complement(180..2156)
                     /locus_tag="VFH91_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6863534.1"
                     /translation="MSAAGLNINRNHFGQVYMWSLVFAGAAITLVSIYELPFQELDAR
                     FIFLCLMVASSSLIAIKIPRVSGRITVADTFVFLTMLQYGGPAAILVSALEGVCATLI
                     ISKRPRTILFNAAILATSTFFTAAVLYWTVGSPVNVVAEGFSANFFLAICIMALVQYA
                     ANTSLIAVEKASKIKESPWNTWRTYYLWTSVTYFAGASAAGIIAILIRTYGFYAVVAT
                     VPIILIICFTYQTYLKNIENSVAQTEAARLHVEELSRYISELQRSEEERGQLLLRAER
                     ARAEAEAANRIKDEFLATLSHELRTPLTSLLGWSSVLREPNQDEKVLTQGLEAIDRNA
                     RVQAQLIDDLLDVSRIVSGKLNLDVRPLDISSVARAAISVVRPAADAKGITLEYSAQP
                     GLGAISADSARMQQIIWNLLSNAVKFTPHGGKIVVRIDQEGPNARVMIRDTGLGIDPK
                     FLPRVFDRFRQADSSTTRSYGGLGLGLAIVRHLVELHGGTVYAESEGPGKGATFSATF
                     PLLAVRAEAIALSGEFNLAEMSSLTGLRVLLVDDEPEARQIITTVIARTGALVQSCTS
                     ASEALSKLIEWRPDVLLSDIAMPEEDGYSFIGRVRALPKEKGGDTPAAALTAYARDED
                     RRQALDAGYQMHIAKPISAPELVMMIAKLAKRLN"
     gene            complement(2283..4217)
                     /locus_tag="VFH91_10825"
     CDS             complement(2283..4217)
                     /locus_tag="VFH91_10825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET6863535.1"
                     /translation="MKRFTHVICWSVFLALLFSSPFFVERKTRAESNSASHRRKVSKD
                     LIKKVENGKGSDFVRVIVQPMAQADRSIDSALKGSGGSNIKKFKNFGVRVVTIPANAA
                     VALASRSDVYYVSLDRDVRPLGHVSRTTGANKVRAGSVISPSGLDGTGVGIAVLDSGI
                     DSQHKSFLDSLGVSRVVYSEDFTGEDRTDDPYGHGTHVASLAAGNGRISNGDYIGIAP
                     NANIVNLRVLNSEGVGTTAGVLRALDWVASNHQSYNLRVVNMSLGMPAIDSYRDDPIC
                     RAVRSLVDSGVVVFAAAGNNGKDEQGHKIYGRIHSPGNEPSAITVGATNTFGTDERSD
                     DGIASFSSRGPTRSFYTDDAGVRHYDNLIKPDLVAPGNKLIDAEADGNYLVTQNPALD
                     AGVSHFDNRRMMYLSGSSMATPIAAGAAALMLQANPSLTPNLVKALLAYTAQPLAGFN
                     MFEQGAGQVNIAGAVQLAQLVRTDLNASTAVGEPLLTGVPPGSQTAIADYTFPWSRGI
                     VLNYNYATGVNLITKYQLIYGTGNLLGSGVITGDGILTLDPAFFSDGVITGDQIVTSD
                     GVITGDGAAFLATYLLFQDLLSDGSFLSDGVITGDGVITGDGVITGDLVTTAQSAMLN
                     GDDGSMMTAAPDNGTDCLDY"
BASE COUNT         1153 a         1178 c         1118 g         1074 t
ORIGIN      
        1 tagtcggggt ctctaccgat cgaaatagaa attctcaaat atcaaatgat aattctcaaa
       61 tgattcttct tacgactaac ccctgacctc tgccctcttc ccaactacat gattctggaa
      121 ggtgaatgtc tccctcttca cccgcatgat tctggtgaga gcttgccctt tcccaactgc
      181 taattgagtc gttttgccag tttcgcaatc atcatcacaa gttcgggtgc gctaataggt
      241 ttagcaatgt gcatttgata gccggcgtcg agggcttgtc gacgatcttc gtcccgagca
      301 taagcagtaa gtgccgccgc gggtgtatct ccgcccttct cttttggtag tgcccgaact
      361 cttccgataa agctgtaacc atcttcttcc ggcatggcaa tatcgctcaa gagtacgtcg
      421 ggtcgccact caattaattt cgatagagcc tcagatgctg aagtgcaact ttggaccaag
      481 gcacctgtcc gggcgatgac agtggttatt atctggcgcg cttccggttc gtcatctaca
      541 agcagtacgc gcaatccggt caacgaactc atctcggcaa ggttaaattc gccgctgagg
      601 gcaatcgctt cggcacggac agccaaaaga ggaaaagtgg cggaaaacgt cgcgcctttt
      661 cctggtcctt cactttctgc gtaaacagtt ccgccatgaa gttccaccag gtgccgaacg
      721 atggccaatc ccaatcccaa gcctccgtaa ctcctcgtcg tcgagctatc cgcttgacgg
      781 aatcgatcga agacgcgtgg taaaaacttt ggatctatgc caagacccgt atcgcgaatc
      841 ataactcgag cgttcggccc ttcttgatcg atccgaacaa caattttgcc accatgagga
      901 gtgaacttaa cggcgtttga caggagattc cagatgatct gttgcatgcg agcggaatct
      961 gcggatatgg ctcccagtcc aggttgcgct gagtactcga gtgtaattcc ttttgcgtct
     1021 gcggccggac ggaccacgct gattgcggcc cgagcgactg aggaaatgtc cagcggtcga
     1081 acgtccagat tcagcttgcc agagacgatt ctggaaacgt cgagcaaatc gtcaatcaat
     1141 tgagcctgaa cgcgagcatt tcgatcaatc gcttcaagac cctgagtcag cactttttcg
     1201 tcctgatttg gctcgcgcag cacactggac caaccaagca atgaagtcag cggggtgcgt
     1261 agctcatggg acagtgtcgc cagaaactcg tccttgatac gattggctgc ctccgcttca
     1321 gcgcgagctc gttcggcacg cagcagcaat tggccacgct cctcttcaga acgctgtagt
     1381 tcgcttatgt atcttgaaag ttcttcgaca tgcagtcgtg ccgcctctgt ctgtgccacc
     1441 gagttctcta tgttcttcaa atacgtttga taggtgaaac agatgatcaa aatgattggg
     1501 acagtcgcga ccactgcata gaagccatag gttctgatca ggattgcgat tattccggcc
     1561 gcagatgctc ctgcaaaata ggtgaccgaa gtccacaaat agtaagttct ccacgtattc
     1621 cagggagact ctttaatctt cgaagccttt tcaacggcta ttaaactagt gttggctgcg
     1681 tattggacca acgccataat gcagatagcc agaaagaaat tggcagaaaa gccctccgct
     1741 acaacgttaa ccggagagcc aacagtccag taaagaactg cagccgtgaa aaaggtggaa
     1801 gtggcaagaa ttgcagcatt gaacaagatc gtgcgaggac gtttgctgat gatcaaggtc
     1861 gcgcaaacac cctcgagggc ggaaaccagg atagctgccg gcccaccgta ttgaagcatc
     1921 gtgagaaaga cgaaagtatc agcaacggtt attcgtccac taacacgagg tatcttgatc
     1981 gcaatcaggg aactcgaagc gaccatcaga cagagaaaga taaatcttgc gtcgagttcc
     2041 tgaaacggaa gctcatatat tgagacaagg gtgatggccg ctcccgcaaa aaccagcgac
     2101 cacatgtaca cctgtccgaa atgatttcga ttgatgttta gtcctgccgc actcatcgct
     2161 agccctcgtc gattgttgtg ataccggttg cttcgaggga agcaaccggt gcatccacca
     2221 aactgtgtgt tcagcgtgtg agagaattcg acaacaatac cgagaacgat gagactagaa
     2281 ggtcaatagt ccagacagtc agtgccgttg tctggtgccg cggtcatcat tgaaccatca
     2341 tcgccgttga gcattgctga ttgggcggtt gtcacaaggt cacctgtgat tacgccgtcc
     2401 ccagtaatta ctccgtcccc ggttataaca ccgtcactca aaaaagaacc gtctgacaac
     2461 agatcctgga aaagtaaata agtagcgagg aacgcggctc cgtctccggt aataactcca
     2521 tcgctggtga caatctgatc accagtaatt actccatcag agaagaacgc cgggtccagg
     2581 gttaaaatcc cgtcacccgt aataactcca cttccgagta agttccctgt gccatagatg
     2641 agctgatatt ttgttatcag gtttacacct gtagcgtaat tgtaattaag aacaatcccg
     2701 cgggaccatg gaaacgtgta atccgcaatc gccgtttgcg atccaggcgg cacaccggtg
     2761 agcagcggtt cgccaacagc agtagaggcg tttaagtcgg ttctgaccaa ttgagccaat
     2821 tgaaccgcgc ctgcgatatt cacctgtccc gctccttgct cgaacatatt aaaccctgcc
     2881 aatggctgtg cggtgtacgc caggagagcc ttaacaagat ttggagtgag actcgggttc
     2941 gcctgcagca tcaaggccgc ggctccggcg gctatcggag tcgccatcga agagccactc
     3001 aagtacatca ttcgccgatt gtcgaagtga ctaacgccgg catcaagcgc aggattttga
     3061 gtaaccagat aattaccgtc cgcttcagcg tcgatcaatt tattgcccgg cgctaccaga
     3121 tccggcttga tgagattgtc gtaatgcctg acacctgcat catccgtgta aaaactacga
     3181 gttggaccac gcgaactgaa tgacgcgatg ccgtcatcag atcgctcgtc agttccaaag
     3241 gtgttggtgg ccccaactgt aattgccgat ggttcatttc caggtgaatg tatccggccg
     3301 tagatcttat ggccttgttc gtctttgcca ttatttccgg ctgcagcaaa aaccacaaca
     3361 cctgaatcaa caaggctgcg caccgcacga cagattggat cgtctcggta cgaatcgatc
     3421 gctggcatac ccaaactcat gttcacgacg cgtagattgt aactttggtg gttcgaagcg
     3481 acccagtcca gggcgcgcaa taccccggca gtcgttccca ccccttcgga attgagtacg
     3541 cgaagattga cgatgttggc attcggagcg attccgatgt agtcgccgtt ggatatacga
     3601 ccattaccag cagcaagaga agccacgtga gtaccgtgcc cgtaagggtc atcggtacga
     3661 tcttctccgg taaagtcttc gctatacacg actcgagaaa cacccaaact atcgagaaac
     3721 gatttatgct gactatcaat accggaatcg agcacggcaa taccgacgcc ggtcccatca
     3781 agaccggagg ggcttatcac gcttccggct cgaactttat tcgcgcctgt ggtcctggac
     3841 acatgtccaa gtggacgcac gtcacgatct aacgaaacat aataaacatc cgatcgagaa
     3901 gctaaagcta cggcggcatt cgcgggaatg gtaactacac gcacaccgaa gtttttgaac
     3961 ttcttgatgt tgctcccgcc tgaacctttc agtgctgaat ctatggagcg gtcagcttgg
     4021 gccatgggct gtacgatcac cctgacgaaa tccgaacctt ttccattttc aactttctta
     4081 attaagtctt tcgatacttt tcggcggtgt gacgccgaat ttgattcggc gcgggtcttt
     4141 ctttcaacga agaatgggga ggaaaacagg agtgctaaaa agacactcca acagattacg
     4201 tgggtgaaac gcttcatctg gtactccgag agacctgtta atcgaggaca gtctcgcttc
     4261 tggtcaaaac aattacactc ggggagagga ctaggctaac gtcggggcag aacactatct
     4321 tgggggactg gcagtctagc agagggtaaa aaatgtttca acaccccggc tacgggcatt
     4381 ttgaggcccg gttttagggt aggattcaac cgttttagat ccacagatta cgcagatttc
     4441 accgattttt agaacgcgaa cgagttgagc tgaaactcca atcacttgag ctgaaaatcg
     4501 gtgtaatctg cgtaagctgt gga
//
LOCUS       DASSFP010000481         6385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1185027, whole genome shotgun sequence.
ACCESSION   DASSFP010000481 DASSFP010000000
VERSION     DASSFP010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185027"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFH91_10830"
     CDS             complement(<1..181)
                     /locus_tag="VFH91_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863536.1"
                     /translation="MSRIIQMRRGQSGKNTQTLYWDSQKPSLQSTISNSLFIWRRRRL
                     RDYQSRKASFRPTVSS"
     gene            656..2017
                     /locus_tag="VFH91_10835"
     CDS             656..2017
                     /locus_tag="VFH91_10835"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HET6863537.1"
                     /translation="MATSVLAPPRRPEIKTELPGPNARKIIEADHRFVNPSYPRPDFK
                     LVAEKAKGVWVEDVDGNIFLDCNAGVAVCSTGHCHPEVVKAIQQQAEQLIHMCGTDYY
                     YRHMPALAEKLDAIVPVPSPTKTHFANSGTEAVETALKLAMHATGREKFIAFFNSFHG
                     RTLGSLSLTSSRAAQRRGFKRQALDVVHVPYPNEFRNPFSAADCGDGGASQGALNWIE
                     ERLFKTTTPPDEVAAIVVEAVQGEGGYVPAPKDFLKGLRRICDQHGILLVVDEVQSGM
                     GRTGKMFATEYYDVQADIICIAKGIGSGLPIGACVARADIMNWKPGAHASTFGGNPVC
                     IAAALKTIELLEGGLVQNSAEVGAYLKAGLEKLKAKHDCIGDVRGLGMMIGVEFVQDR
                     RSMKPAPELRDRIEIASFERGLILLGAGTNSIRWSPPLILTKENVDVALEIFDAAIGA
                     SIK"
     gene            complement(2272..3516)
                     /locus_tag="VFH91_10840"
     CDS             complement(2272..3516)
                     /locus_tag="VFH91_10840"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET6863538.1"
                     /translation="MNILVLNCGSSSVKSQIIATDLSLIAANGDQRLARGNIERIGGE
                     AIVSLQSSDREKQLSTATLRDTRAAVEFIVRWAGSEDGPKALTSVADIHAVGHRVVHG
                     GEHFKQSVLIDDKVIRAIEDCIELAPLHNPTNIKGIQAARDVLGAGLPQVAVFDTAFH
                     QTLPEHAYLYAIPYQLYRRHRIRRYGFHGTSHRYVAYRYRQLRGITRDETDVITLHLG
                     NGCSIAGIRGGNSQDTSMGLTPLEGLVMGTRSGDLDPAVIDFIGEKEGLKSGEVETLL
                     NKQSGLLGISGLTNDMRELLAEEQENNDRRAHLAIEIFCYRARKYIGAFLAATGGASA
                     LIFTGGIGENSPDVRARICDGLEWMGLELDDEKNALAINGYEGLISKDASRLACYVIP
                     TDEELLIARDTLRCVQKVPQRY"
     gene            complement(3497..4870)
                     /locus_tag="VFH91_10845"
     CDS             complement(3497..4870)
                     /locus_tag="VFH91_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6863539.1"
                     /translation="MTTIYAARWLLPVASQAVEDGALVVEGSKIVGVGSRAKLLKLFP
                     ESSVQDLGNAIILPGLVNAHSHLELTAMRGYLEDEETNFFSWLRKLTLARLNLMTEDD
                     IEVSALWGACEAARAGVTAVGDASDAGRLSMKALQSVGLRGIVFQESFGPDANLAKEN
                     FVTLQAKVDQLLASQTELVRVGVSPHSPYTVSGPQLELIADFALSEKLPLMIHAAESA
                     AEESLIKDGTGLFAEGLQKRNIVWDIPGMSPIQYLKERGILETKALLAHCIRVDQHDL
                     EAISADGASIAHCPKSNAKLGHGFARLSAFLQNGINVGIGSDSVASNNTCDILEETRF
                     AALLSRAVGSGDNTQSRVSNPELLHLATSGGARCLGLNKVGPLQPGADADFIAVSVDG
                     TQQTPMYDPAATLLFSSSGRDVVLTVVAGREIYRNGRVETVDEERLSARINEISRKLS
                     DHEYSGS"
     gene            complement(4878..5552)
                     /locus_tag="VFH91_10850"
     CDS             complement(4878..5552)
                     /locus_tag="VFH91_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET6863540.1"
                     /translation="MFRQYLPQLIVALKLVVAIICGGAIGLERELSRKAAGLRTNVLI
                     CMGAALYMIVSRHIGGGAPYTDPARLAAQVVTGIGFLGAGVILQSRGSVTGLTTAATI
                     FVVGAVGIAVGEGLFGIAVFTTALIIVVLVLLRSVERAVIKRRRMFHYTMTSRDPSEL
                     LEQLLDLFEREHVHLEDFEVRELGGGEHEVKVSVVTSLDGNRRLLRQLRTLGGELLTT
                     IQHRLG"
     gene            complement(5579..6175)
                     /locus_tag="VFH91_10855"
     CDS             complement(5579..6175)
                     /locus_tag="VFH91_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6863541.1"
                     /translation="MKRAVVFAVLVLTIILSAFFLNYYWIHRYDSLIARQAAIYRVDP
                     DLVWSIIYEETYFSPWKIGKDGEIGLMQVTPTVGKEWAAATGMKQFERQMSTDPGAFL
                     AEAEHNIQVGCWYLEKIHEQFQDATDSETRMVAAYNAGPSRVVEWSRVAQDAKPQTSA
                     EFIARIDIPSTRAYVISIMQRYRQLKTAKNQAITVHNP"
BASE COUNT         1578 a         1708 c         1574 g         1525 t
ORIGIN      
        1 gtgaggaaac agtcggacgg aaactggctt ttcgtgattg ataatcccga aggcgccgcc
       61 tccgccagat aaacaagcta tttgagatgg tggattgcaa cgacggtttc tgactatccc
      121 aatacaaagt ttgagtattc ttgccgctct gcccccttct catctgaatg attctactca
      181 ctggttcagt ctccatcggc tcaactctgc ggcactctgc ggttctctgc gtatctctgc
      241 gatgcttcag ttacaaccaa ggtgagttct acattggact cagaaagacg cagagtgacg
      301 cagagagtcc cgctaagaag tgccgcagag aaaagatctt ctcagaatct tgtggttttg
      361 gaagcgggcg agcttgccgc actctcagaa atgaaaaatg atcaatggcc aatgatcaat
      421 gaccaataag gggtttcgct ccaatctagc tcaattcccg gtcacagtgc tagaatgcct
      481 acaactctta tgtttaccca gcatccgcta catttcaggc cggttgaatc gcgagcgaac
      541 ctcttcgctg actagtcctt tattccaata aacccgttcg tgagtcgagc attcgctcga
      601 ccaagccttc gtcaccgatt tacaacccgg ttttattgaa taaggagtta accctatggc
      661 taccagcgtt ttagcaccac cccgtcgtcc ggaaatcaaa acagaactgc cggggcccaa
      721 tgctcgcaaa attatcgaag ccgaccaccg attcgtgaat ccgtcctatc cgcgaccgga
      781 cttcaagtta gtcgctgaaa aagcaaaggg cgtctgggtt gaagatgtcg acggcaatat
      841 cttcctcgac tgcaatgcag gggtcgccgt ttgttcaacc ggccactgtc accccgaagt
      901 cgtaaaggca atccagcaac aggctgagca gcttatccac atgtgcggta ccgattacta
      961 ttaccgccac atgccggcgt tggctgaaaa actcgacgct atcgttccgg ttccgtcgcc
     1021 caccaaaacg cactttgcga acagtggcac tgaggcggta gaaacggctc tcaaactggc
     1081 gatgcatgct accggacgcg agaagttcat tgctttcttc aatagctttc acggacgtac
     1141 tcttggatca ctgtcgctga catcttctcg agccgcgcag cgaagaggat tcaaacggca
     1201 ggcgttggac gtggtccacg ttccctatcc aaatgaattt cggaatccat tcagcgctgc
     1261 agattgtggt gacggcggtg cttcacaggg tgcattgaac tggatcgagg aaagactctt
     1321 taagacgacc acgcctccgg atgaagtagc ggcaatagta gtcgaggcgg tccagggcga
     1381 aggtggatac gttcctgctc caaaagattt tcttaaagga ttgaggcgaa tctgcgacca
     1441 gcatggaatc ttgttagttg tagacgaggt tcagtcaggc atgggccgta ctggaaaaat
     1501 gtttgcaaca gagtattacg atgttcaggc ggatatcatc tgcatcgcca aaggtattgg
     1561 ctcaggactc ccaatcggag cctgcgtcgc gcgtgccgac atcatgaatt ggaagccggg
     1621 tgcacacgcc tctacgtttg gtggcaaccc ggtctgtatt gctgcagctt tgaaaacaat
     1681 tgaactcctc gaaggtggtt tggtccaaaa cagcgccgag gtaggcgctt atctgaaagc
     1741 aggccttgag aagctcaaag caaagcacga ttgcatcggc gatgtgcgag gtctcgggat
     1801 gatgattgga gttgagttcg ttcaagaccg aagatcgatg aagccagcac cagagttacg
     1861 agatcggatt gagatagcga gttttgaaag aggactgatt cttctgggag caggaactaa
     1921 ttcaattcgc tggtcgccgc ctttgattct gaccaaagaa aacgtcgacg tcgctctgga
     1981 aatattcgac gccgctattg gcgcctcgat taagtagtgg ttttttactc cgccaagtta
     2041 cgtgtttata gcccaccgaa gcccttaccc tcccgcgccg ttcggaggta agggcttcgg
     2101 tgctataaac atgtcacccc tgccggggtg aaaacccgct gacattctcg tgttcaagaa
     2161 atcagcggac aatgtcattg gtggccgctc tgccgttctt cgtatctccg actcctgcat
     2221 tgatcatggg ttagatgggt caccgccgag atgcggagtt gagagaagag cctaataccg
     2281 ttgcggcacc ttctgtacgc acctcaacgt gtcgcgtgct attaacagct cctcatcagt
     2341 gggaatcaca taacacgcca gccggcttgc atccttgctg atcaagcctt catatccatt
     2401 gatggctaga gcgtttttct catcatcgag ctccagcccc atccactcca gaccgtcaca
     2461 aattcgcgct cgaacgtcgg gagagttctc tccaattcca cccgtaaata tcagagcgct
     2521 ggcgccacct gtagccgcga gaaacgcacc aatgtatttg cgagcgcgat aacagaaaat
     2581 ttcgatggcg aggtgggcgc gccggtcgtt attttcctgt tcctccgcca gtaactcccg
     2641 catgtcattc gtcagtccgg agataccaag taaccctgac tgcttattta acagcgtctc
     2701 aacctcgcct gatttgagac cttccttctc gccgatgaag tcaatcactg caggatcgag
     2761 atcgcccgac cgcgttccca tcaccaaccc ttccaaaggc gtcaatccca tcgaggtgtc
     2821 ttgtgagttt ccgccgcgaa taccggctat cgaacatccg tttcccagat gaagtgtaat
     2881 gacgtcagtt tcgtcgcgcg ttattccgcg gagttgtcga tagcgatagg caacatagcg
     2941 gtgtgacgtg ccgtgaaaac cataacgacg aatgcgatgc cggcgataaa gttgataagg
     3001 aatcgcgtag agatatgcgt gctcgggaag cgtttgatga aacgccgtat cgaacactgc
     3061 tacttgcggc aatcccgcgc cgagcacatc tcgagccgcc tggatacctt taatattagt
     3121 tggattgtga agcggtgcga gctcaataca atcttcgata gctcgaatta ctttgtcgtc
     3181 gatcaaaacc gactgcttaa aatgctcgcc gccatgaaca actctatgac cgaccgcatg
     3241 aatatcagcg acactggtga gagcttttgg accatcttcg gagccagccc aacgtacaat
     3301 gaactctaca gccgcgcgcg tatctctgag cgtagcagta gagagctgtt tttcgcgatc
     3361 acttgattgc agagagacta ttgcctcgcc gccaattctc tcaatgttgc cgcgcgctaa
     3421 cctctgatct ccattggcag ctatcagcga cagatcagtg gcaatgatct gagatttgac
     3481 agaggatgaa ccacagttaa gaaccagaat attcatggtc cgatagttta cgcgagattt
     3541 cgtttattct cgcactcaag cgctcttcat caacggtttc aactcggcca ttccgataaa
     3601 tctcccgacc cgccacaacc gtcaatacaa cgtcacgtcc tgaggacgag aacaaaaggg
     3661 tcgctgcagg atcgtacatg ggcgtttgct gtgttccatc gaccgaaaca gctatgaaat
     3721 cagcgtcggc gccaggctga agtggtccaa ctttattcag gcccaaacat ctagcccctc
     3781 cactcgtcgc caggtgaagc agttccgggt tgctaactct actttgagtg ttatcgccgg
     3841 aaccgacagc tcttgataac agcgccgcaa aacgggtttc ttcaagaata tcgcaggtat
     3901 tgttactcgc aaccgagtcg ctaccgatac caacattgat accattctgc aagaacgcac
     3961 tcagccgagc gaatccgtgg cccaacttgg cgtttgattt tggacagtga gcaatgctgg
     4021 caccgtccgc agatattgct tcaagatcgt gttgatccac gcggatacaa tgagcaagta
     4081 atgccttagt ttcgagtatc cctcgctctt tcagatactg aatgggcgac ataccgggaa
     4141 tgtcccaaac gatatttcgt ttctgcaaac cttcggcgaa cagtcccgtt ccatccttga
     4201 ttagtgactc ttcagcggcg gattcggctg catgaatcat caacggcaac ttctcactta
     4261 aagcgaagtc cgcaatcagt tcgagctgag gtccggaaac tgtgtaaggt gagtgtggag
     4321 aaacgccgac gcgtaccagc tcagtctgac ttgccagtaa ctggtccacc tttgcctgta
     4381 gcgtgacaaa gttttcttta gctagattcg cgtctggtcc aaatgattcc tgaaagacga
     4441 ttccccttag accaaccgac tggagagctt tcatactgag tcggcccgcg tcgctggcgt
     4501 ctccaacagc cgttactcct gcccgtgccg cttcacacgc accccataac gcggatactt
     4561 caatatcatc ttccgtcatc agattaagcc gcgctaaggt cagtttccga agccatgaga
     4621 agaaattggt ctcctcgtct tcaagataac cacgcattgc ggttaactct aaatggctat
     4681 gtgcattgac caggccaggc aggatgatcg cattaccgag atcttgaact gaggactccg
     4741 ggaaaagctt taacaacttg gcacgcgagc cgacacctac gatcttgctt ccctccacta
     4801 cgagcgcgcc gtcttcaact gcctgagagg ccacaggtaa aagccagcgt gctgcgtaga
     4861 ttgtcgtcac agtaacctta accaagccga tgctgaatgg tcgttaatag ttctccgccg
     4921 agcgttcgaa gttgtcgcag tagccgccgg tttccgtcaa gcgatgttac aacactcacc
     4981 ttaacttcat gctcaccacc gcctagctcg cgcacttcga agtcttcgag atgaacatgc
     5041 tcgcgctcaa atagatccaa aagctgttcg agtagttcgc tcggatcgcg tgatgtcatt
     5101 gtgtagtgaa acattcgccg gcgcttgata acagcgcgtt ctacggaacg cagcaaaaca
     5161 agtacgacga tgatcagggc ggtggtaaaa acagcaatac caaaaagacc ttcgccaact
     5221 gcaatgccga ctgccccgac cacaaagatt gtggcagcgg ttgtcagtcc ggtaacagaa
     5281 ccacgggact gcaaaattac gccagctccc aggaaaccga taccagtcac tacttgagct
     5341 gccagtctgg ctggatctgt gtagggggcg ccgcctccga tatggcgcga gacgatcatg
     5401 taaagcgccg cgcccatgca aatgagcaca ttggtcctga gtcccgctgc cttacggctc
     5461 agctcgcgct cgagaccgat ggcgccgcca cagatgattg cgactacaag ctttaaagct
     5521 actatcagtt gaggcaagta ttggcgaaac aaagtctctc cgtacgtcga gaaacagact
     5581 aaggattgtg aacagttatc gcctggtttt tcgcggtttt gagttgacgg taacgctgca
     5641 taatcgagat gacgtacgcc ctggttgacg gtatgtcgat gcgtgcgatg aactcggcgc
     5701 tggtttgcgg cttcgcgtct tgggccacgc gactccattc aactactcga ctcggtccag
     5761 cgttgtaggc ggctaccatt cgagtttcgg aatcagtcgc atcttgaaac tgttcgtgga
     5821 ttttttccag gtaccagcac ccaacctgaa tattatgttc ggcctcggca aggaaagctc
     5881 cggggtcagt agacatctgt ctctcaaact gcttcatgcc tgtagctgcc gcccactctt
     5941 ttccaacagt tggcgtaact tgcattagac caatttcgcc gtccttacca atcttccagg
     6001 gactgaaata ggtctcttcg taaataatgc tccaaacgag atcgggatcg acgcgataga
     6061 ttgcggcttg ccgggcaatc aacgagtcgt atcgatgaat ccagtagtag ttgaggaaaa
     6121 aagcagaaag aatgatggtg agtactagaa ccgcaaaaac taccgctcgt ttcatcaagt
     6181 ttcacaagac gctgctcgaa cgattgagag tgagctcaaa atcttccgtt gcgacgagta
     6241 gtttaatttt ttcgtttgag agtcgaccag aaactggctt gcgaacaaga gtagtcaact
     6301 ccctcgtaac gcgaacgaag agcatgttca tcgagcacgt gaccttgtaa ccaaacttca
     6361 gcttcgagag tagatccaac actca
//
LOCUS       DASSFP010000482         6380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1473892, whole genome shotgun sequence.
ACCESSION   DASSFP010000482 DASSFP010000000
VERSION     DASSFP010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473892"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VFH91_10860"
     CDS             complement(<1..1298)
                     /locus_tag="VFH91_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6863542.1"
                     /translation="MFDLAKSNTTDHERTILRKLRVWIAALAVACLLAGIGIGAMLSG
                     RPTVAQDGLQIARAPEALSASFAEIARRVEPAVVNIETVQKSIDLTDKSADEDSEDQT
                     ANPLLDMFRRQQRRPTRGVGSGFIVNAKGYILTNNHVIEDASRIIVGLQSGEKYRGTV
                     VGVDSATDVAVIKIDAQHELPTVTLGDSNAAQVGDWVLAMGSPFGLDQTVTAGIISKK
                     ERESPFFNVFQRFLQTDAAINRGNSGGPLVNMRGEVIGMNSQIATSTGDYNGIGFALP
                     ANETNFVYKQIVSQGKVKRGYLGISLESVHDEFAKVYGLPETKGAIVTSVTPTESGQA
                     TPAAKAGIQASDIIVEFEGSPVASAPDLIQRVASTPVGQQTTLVFLRDTNGKLEKKTV
                     SVVLSERPNTDDNAKDWVEPTKSPSKSPDPKGNALHLGITL"
     gene            complement(1575..2558)
                     /locus_tag="VFH91_10865"
     CDS             complement(1575..2558)
                     /locus_tag="VFH91_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6863543.1"
                     /translation="MPTPEVRHFEMLALRVEKKRLSVQEIPKLKPEGEALVRVALSGI
                     CNTDLEIARGYAGFNGTIGHEFVGVVEKSSDKSLTGERVVGEINAGCGKCQLCLSGDS
                     RHCPNRTVLGIVGRDGAHAEFLRLPAKNLLRLPKKISDEQAVFIEPLAAACGILERVS
                     FNPGYRVAVIGDGKLGLLCAQTIGLQKCVTTLIGKHRNKMRVVAQRGIETISPATASK
                     RKNHFDVVVEASGSPSGFALALDLLRPKGTLVLKSTFHDVTKMDAARIVVDEITIVGS
                     RCGRFKPAIDLLVKGEVDVESLISEEQALADGVYAMQRAGERGVMKVLLRP"
     gene            complement(2530..3030)
                     /locus_tag="VFH91_10870"
     CDS             complement(2530..3030)
                     /locus_tag="VFH91_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863544.1"
                     /translation="MKLKNLSRGITISLLRVGLVGLLFGGCWLIYRTLPQSEIAPPDQ
                     GTTNVQIILHQPAESAFQTLQASVEFSPVDVVAVQHEFFAEPRAGQRFDDFFKERMKG
                     RSKVKTELDKQGHGSVLLTPGSWWLRAKLSGDEEIEWRLPLKISGTRQVIELTPQNAY
                     TRSKTF"
     gene            complement(3059..3427)
                     /locus_tag="VFH91_10875"
     CDS             complement(3059..3427)
                     /locus_tag="VFH91_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6863545.1"
                     /translation="MSLKIVFSSFVFVALAGLCVSVNSNARGSDTAGQARRTASRMYG
                     QYCIACHGSDGRSQTKKGKYNHARDLSDAQWQDDVSDERMFNSIMNGRNIRGNMPGFK
                     DKLNEQEAEALVEYVRRFKK"
     gene            3632..5056
                     /gene="purF"
                     /locus_tag="VFH91_10880"
     CDS             3632..5056
                     /gene="purF"
                     /locus_tag="VFH91_10880"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET6863546.1"
                     /translation="MAEIEITKLREECGVFGIYGNSEANRLTYLGLYALQHRGQESCG
                     IVASDGSELRLEKAMGHVSEAFNQSQLDRLRGASAIGHVRYSTAGEVSILEAQPFLVN
                     CQHGQIAICHNGNLPFASEDRRRLEQEGAIFSSTSDTEIVLHGVARSQLPIREAIPQV
                     LRETEGAFSMLFLTPNELIAIRDSRGFRPLALGRLGEAWVVASETCAFDLIDAQYVRD
                     VEPGEMIVIDQNGLRSSHPLPKEKHSMCLFEHVYFARPDSLIYGRSINQSRHKMGRQL
                     AVEQPADADIVVPVPDSGVAASVGYAAESGLSFRFGLVRNHYVGRTFIEPRQSIRSFG
                     VRIKLNPVRDLINGKRVVLIDDSIVRGTTSKKIVRMVREAGAKEVHVRISCPPTISPC
                     YYGVDTPDKSELIAAQMSVEEICKFIEADSLGYLSLEGMITATGLNANSVCVACWNGN
                     YPTRITREAETMWERDHESVAAEG"
     gene            5175..6311
                     /locus_tag="VFH91_10885"
     CDS             5175..6311
                     /locus_tag="VFH91_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET6863547.1"
                     /translation="MRRATRGCGCAIAVFLVLASIGGYLGYKFVYPSWKTRNPPPSGA
                     ELTIKILDVGPVDGDSILIIAPSGVKVLIDAGDVSRGKSVVEALKKNNIQQLDYFIAT
                     HPHPDHIGGAAEVFKAVKVLNVIDNGEGPVVPPSAVVAKPASAKTSKKPAAPVKPNPK
                     TASVTKFYDDYQAAIKGGGVQYEKAQPGKKYDLGGGAFLTILGPTEPYFTKDQMKSGG
                     NEPNANSIIARLDYGSFSMLLPGDAEEQTEHRLLTKDLNLQARVLKIAHHGSKYATTD
                     DFLARVKPEIAIISCGAWNRYGHPHQSVLDRLKAANVKLYRTDLQGEITLTSRGGAGD
                     ISVKTAKEPSEDLWTGRLAQKDDSSRSGFIAYGDFGPPPKPRKQ"
BASE COUNT         1559 a         1607 c         1591 g         1623 t
ORIGIN      
        1 agggtaattc caagatgcaa tgcgttgccc tttggatcag gactcttaga aggtgattta
       61 gttggttcaa cccagtcctt cgcattgtcg tcagtatttg gtcgttcgct aagtacgacg
      121 ctgacggtct ttttctcgag cttaccgtta gtgtcacgca agaacacgag ggtcgtttgc
      181 tgacctactg gagtgctcgc aactcgttga atgagatctg gggcgctcgc aactggactc
      241 ccttcaaatt caacaatgat atcgctagct tgaataccag ctttagctgc cggtgtagct
      301 tgaccacttt cagttggtgt tactgaagtg acgattgcgc ctttggtctc tggaagtccg
      361 tagacttttg caaactcgtc atggactgat tcaagtgata tgccgagata accgcgtttg
      421 acttttccct gactaacaat ctgtttatag acgaagttgg tttcgttcgc cggaagggca
      481 aacccaatgc cgttgtaatc gccggtcgat gtcgcaatct gggagttcat cccgattacc
      541 tcaccacgca tgtttaccag tggtccaccg ctgttgcccc gattgatggc ggcgtcagtt
      601 tgaagaaatc tttggaagac gttgaagaac gggctctcac gttccttttt ggaaatgatt
      661 ccggcagtca ccgtttggtc caatccaaat ggagaaccca ttgccagtac ccagtcacca
      721 acctgggcgg cattcgagtc gccgagtgta actgtgggaa gttcatgctg tgcgtcgatc
      781 ttaatgactg ccacgtccgt agcagaatct acaccgacta cggtaccgcg atacttttca
      841 ccggactgca atccgacaat gatgcgactg gcatcttcga tcacatgatt gttagtcagg
      901 atatagccct tcgcattgac gatgaatccg ctgccaacac ctcttgtcgg acgtcgttgc
      961 tggcggcgaa acatgtcgag cagtggattc gcggtctgat cttcgctgtc ttcgtcagcg
     1021 cttttatcag tcaggtctat ggacttttgg acagtttcga tgttgacgac ggctggttcg
     1081 acgcgacgtg cgatttcggc aaacgaggca gagagcgcct cgggcgctcg ggcgatctgc
     1141 aacccatcct gcgctaccgt aggtctaccg gacagcattg cgccgatacc gattccggcg
     1201 agcaagcacg cgacggcaag ggctgcaatc cagactctca gctttcgcag aatagttcgt
     1261 tcatggtctg tagtattgga ttttgcgagg tcaaacatag ctcaccacct tcgaagtaac
     1321 cgaaaccgct gtgctctcgg ggacacaccc accgttaatg gcaggaatca gagcacaagg
     1381 aaagggttag agtatagcta aagggtcggc tgcgggcaaa aagctttgga tatctgttag
     1441 ataacgccag attcttctag tgatcgagca agtttgtgaa gcccctctac cgtcagcgtc
     1501 aatgaaacag ctcacagaat accccacacg ggagtggatg cattcaagtc caacctacac
     1561 gagagacaac ctggttaagg ccgcaggagg actttcataa ctcctcgctc gcctgctcgt
     1621 tgcatcgcgt acacgccgtc ggcaagtgct tgctcttcgc ttatcaaact ctcaacatcc
     1681 acttcgccct tcaccagtaa atcaatcgca ggtttgaaac ggccacagcg cgagccaaca
     1741 attgttatct cgtcaaccac aattctcgcc gcatccattt tagtaacatc gtgaaaggtc
     1801 gactttaaaa ccagggtccc tttaggacgt aagaggtcaa gcgcgagcgc aaatcccgaa
     1861 ggcgatccag aggcctcaac gacaacgtcg aaatggtttt tccgtttgga agctgttgcc
     1921 gggctaatcg tctctatccc tcgttgggcc acaactctca tcttgtttcg atgtttcccg
     1981 ataagtgtag ttacacattt ttgcagtccg attgtctgcg cgcacaggag acccagtttg
     2041 ccatcaccaa tcactgctac ccgatacccg ggattgaacg aaacgcgttc gaggattccg
     2101 caggcagcgg cgagcggctc aatgaagact gcctgctcat cactaatttt ctttggcaag
     2161 cggagcaggt tttttgcggg caggcggaga aactcggcat gtgctccatc gcgaccaacg
     2221 atgccaagta cggtgcgatt gggacaatgg cgcgagtcgc cggagagaca taattgacac
     2281 ttaccacatc cggcattgat ctcacccacc acgcgttctc cggtgagact cttatcactg
     2341 ctcttttcga cgactcctac aaactcgtga cctatagttc cattaaaacc agcatatccc
     2401 ctggcaatct ccaggtcggt gttgcaaata ccggaaagag caacgcgaac cagagcttca
     2461 ccttcaggtt tcagctttgg aatctcttga actgagagtc gttttttctc aacgcgcagg
     2521 gctaacatct caaaatgtct tacttcgggt gtaggcattt tgcggcgtca attctatgac
     2581 ttgcctggtg ccactaattt ttaatggcaa gcgccactca atctcttcgt ctccagataa
     2641 cttcgcacgg agccaccagc ttccaggagt caacaacacg gaaccgtgtc cttgtttgtc
     2701 cagctctgtc ttgaccttag accgcccttt cattcgttcc ttaaaaaaat catcaaatct
     2761 ttgtccggct cgcggttcag cgaaaaattc atgctggacc gccacgacat ctactggaga
     2821 aaactcgact gaggcctgta atgtttggaa agcgctttcg gctggttggt gaaggattat
     2881 ctgtacgttc gttgttccct gatcgggagg cgcaatttcg ctttggggca gggtgcggta
     2941 gatcaaccag cagcctccaa aaagaagacc aacaagtccc actcgcagga gactgatcgt
     3001 tatgcctcga ctgagatttt ttaacttcac cacaactacc cgtccgatct tgatcttgct
     3061 atttcttgaa ccggcgaacg tattctacaa gcgcctcggc ttcctgttca tttaatttat
     3121 ccttaaaccc cggcatattg ccacgaatat tccgaccatt catgatcgaa ttgaacattc
     3181 tttcgtcgct aacatcatct tgccactggg cgtccgaaag atcccttgca tgattatatt
     3241 tgcctttttt ggtttgtgag cgcccatctg aaccgtggca ggcgatacag tactgcccgt
     3301 acattctgga agctgttcgt ctggcttgac cggctgtatc cgaacctcga gcgttcgaat
     3361 tcaccgagac gcacaagccc gctaaagcta cgaagacgaa agaactgaaa acgattttaa
     3421 gcgacatcaa agtcctcacg aatctgaata agcagaccga aattaatcat accgaagcga
     3481 atcacatttg tgtattaacc atggagatgt cgtttcgttg caggtttctt gcaacgaaaa
     3541 agaaatctgg gcttctgaag ttgaaacgtt tttgattgcg aagtaagctc atcgagtaat
     3601 ccccaacgct ttcaaatcag gaacaactcc attggctgaa atcgagatca caaagcttcg
     3661 cgaagagtgt ggtgtttttg gaatctacgg aaactcggaa gcaaatcggc tcacgtacct
     3721 cggactttat gccttgcagc atcgaggtca ggagtcctgc ggtattgttg cgtccgacgg
     3781 ttcggaatta cgacttgaaa aagcgatggg gcacgtctcc gaggccttca atcagagtca
     3841 actcgatcgt ctgcgcggag caagcgcaat tgggcatgtg cgttattcga ctgctgggga
     3901 ggtttcgatt cttgaagctc aacctttcct tgttaattgc cagcacggtc aaatagcaat
     3961 ttgccacaac ggtaatcttc cattcgccag tgaggacagg cgccgtcttg agcaggaagg
     4021 cgccattttc tcgtctactt cagacaccga aatcgtgctt catggagttg cacgttctca
     4081 attgccgatc agagaggcca tcccacaagt gttgcgtgag acagaaggtg cattctcgat
     4141 gctcttcctc acgccgaatg agttaatcgc catccgtgat agtcgaggat tcaggccact
     4201 tgcattgggc cgtttgggtg aggcgtgggt tgttgcatct gaaacgtgtg ctttcgattt
     4261 gatcgatgcg cagtacgttc gcgacgtcga gcctggtgag atgattgtga tcgatcaaaa
     4321 cggtttacgg tcgtctcatc cactgccgaa agaaaagcac tcgatgtgct tgttcgagca
     4381 cgtctacttt gcgcggcctg actctctaat ctatggacgc tcgatcaatc agtcgagaca
     4441 taaaatgggc cgacagcttg cagtcgaaca gccagccgat gcggacatcg tggtgccggt
     4501 gcctgattca ggtgttgcag cttcggttgg atatgcagcc gagagcggac tcagctttcg
     4561 ttttggtctc gttcgaaatc actacgttgg caggacgttt atcgagccac gtcaatcaat
     4621 tcgttcgttc ggagttcgca taaaactcaa tccagttcgc gacctcatta acgggaaaag
     4681 ggttgtattg attgacgatt caatcgttcg tgggaccact tcgaaaaaga tcgtgcgcat
     4741 ggtgcgtgag gcgggcgcta aagaagttca tgttcgcatc agctgcccgc cgacaatcag
     4801 tccttgctac tacggagtag atacacccga caagtcagag ctgattgccg cccaaatgtc
     4861 agttgaggag atttgcaaat ttatcgaggc cgattctctg ggttacctct ctttggaagg
     4921 aatgatcacg gcgaccggtc tcaatgcaaa ttcagtatgc gtcgcctgtt ggaatggcaa
     4981 ctatcccact cgaatcacaa gagaggcaga gactatgtgg gagcgtgacc acgagtcagt
     5041 agctgccgaa ggatgaaccc cgttaaatga tcaattgtga tttgagaatt atcagttgct
     5101 ggttttgtgc tcaagttcgc tcacatcaaa attcataatc atcaaatgat aattgatact
     5161 tagaggggtt ttaggtgcga cgagctaccc gaggctgtgg ttgtgcaatt gcggtttttc
     5221 tggtgctggc ttcgataggt ggctacctcg gctacaagtt tgtctatccc tcgtggaaga
     5281 caagaaatcc tccgccatcg ggagcagaac tcactatcaa aattctcgat gttggaccag
     5341 tcgatggtga ttccatcctg attattgcgc cctccggagt taaggtgctg attgatgcag
     5401 gtgatgtttc aaggggaaag agcgtcgtcg aggcgttgaa gaaaaataac atccagcagc
     5461 ttgattattt cattgctaca catcctcatc ccgatcacat cggtggcgca gctgaagttt
     5521 tcaaagccgt taaagttttg aacgtgatcg ataatggtga gggtcctgtc gttccaccat
     5581 cagccgttgt tgcaaaacct gcctctgcta aaacgtcgaa aaagccggcg gcgcctgtca
     5641 agccaaatcc caaaacggct tcagtcacca agttctacga cgactatcag gccgcaataa
     5701 aaggtggcgg agtgcaatat gagaaggcgc agcccgggaa gaagtatgat ctgggtggag
     5761 gcgcatttct gactattctt ggaccaacgg aaccgtactt tactaaggac cagatgaagt
     5821 cgggcggaaa cgagccgaat gcgaactcga ttatcgcccg cctggactat ggttcgtttt
     5881 caatgctgtt gcccggcgac gcagaagagc aaactgagca tcgtttgcta actaaagatt
     5941 taaatcttca agcacgtgtg ttgaaaattg cacatcatgg ttcgaaatat gcgacaaccg
     6001 atgattttct agcacgagtg aagccggaga tcgcaatcat ctcctgtggc gcatggaacc
     6061 gatatggaca tccgcaccaa tccgtgctcg atcgactcaa agcggctaac gtaaaactct
     6121 accgaacgga tctgcagggc gagatcactt tgacaagtcg aggtggcgcc ggagatattt
     6181 ctgtcaaaac cgctaaagag ccaagcgagg atttgtggac tggcaggctc gcacaaaagg
     6241 acgactcttc aaggtcaggc tttatcgctt atggcgattt cggcccacca cctaaaccca
     6301 ggaagcagta gatcgactta gatgactaaa tctatttctt atagtgatgc cggtgttgat
     6361 atcgacgccg caacgcgaac
//
LOCUS       DASSFP010000483         3894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1928367, whole genome shotgun sequence.
ACCESSION   DASSFP010000483 DASSFP010000000
VERSION     DASSFP010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928367"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..454)
                     /locus_tag="VFH91_10890"
     CDS             complement(50..454)
                     /locus_tag="VFH91_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863548.1"
                     /translation="MSSEQARSMAERIARRISADAAVPKAVPRSNFEVSSELAAIREN
                     LSQLQNRLIQIESKIGTASSASQSDQIASSRIHSPWLAGVNTSHPSQERFGVEEAAVS
                     ELVDFFHSEKTCTVEPGGKPCDHCAMCSSRGF"
     gene            complement(632..907)
                     /locus_tag="VFH91_10895"
     CDS             complement(632..907)
                     /locus_tag="VFH91_10895"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET6863549.1"
                     /translation="MILARVVGNVVATKKNRRYESARIMLCQQLTPEGEEMNQTVIAL
                     DSVDSGVGDTVLIVQEGWGASTAATKEAGAAIDSAIVGVVDRVDLLG"
     gene            complement(921..1193)
                     /locus_tag="VFH91_10900"
     CDS             complement(921..1193)
                     /locus_tag="VFH91_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET6863550.1"
                     /translation="MQLARVIGTVVATVKNESLDGRKLMVVQTLDKNLQPVGKPMVAV
                     DSVGAGVGELVFWCRGKEASFPFKREDTPTDCTIVGIVDSDAHVTR"
     gene            complement(1198..1470)
                     /locus_tag="VFH91_10905"
     CDS             complement(1198..1470)
                     /locus_tag="VFH91_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET6863551.1"
                     /translation="MIIARILGTVVSTQKDERLTGKKLLIVRPVNVDGSDTTGYVVAV
                     DTVGAGFHERVLVVAGSSARLAQGMKDVPVDAAIVGVIDTVDVTNS"
     gene            complement(1477..1758)
                     /gene="eutM"
                     /locus_tag="VFH91_10910"
     CDS             complement(1477..1758)
                     /gene="eutM"
                     /locus_tag="VFH91_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HET6863552.1"
                     /translation="MQEALGMVETKGLVAMIEAADAMVKAANVTLVGYEKIGAGFVTA
                     IVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHGSVDETLPIGLER"
     gene            complement(1877..2701)
                     /locus_tag="VFH91_10915"
     CDS             complement(1877..2701)
                     /locus_tag="VFH91_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET6863553.1"
                     /translation="MIDEQTARRDLIQICHLMYERSYVVSSDGNVSLRLDDGRILATP
                     TMTCKGRMTEDLIAVTDLNGKSLNDRKASSELAMHLLIYRERPDVKAVCHAHPPHGTA
                     FAVAGLAIDQPILSEVILTLGCVPLASYGTPSTNELTEAMQPLVKHHNALLMANHGAV
                     AYGSDLWQAWDRLETLEHTAKIAILARVLGGSKNLPADAIEKLINVREASGYLGESAR
                     CQACGYLHETQLTCPTGDRPARRTSYTETTNGSGKISLTREELVELLSQAIHPSDN"
     gene            complement(2719..3315)
                     /locus_tag="VFH91_10920"
     CDS             complement(2719..3315)
                     /locus_tag="VFH91_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863554.1"
                     /translation="MAIRLLPLLLTFFLGLGIAYLVHTNRTATTKAVLGQVDQPLVIR
                     SVPDSEMPESLNRLHMKRAAVSLRAVFDANGKVEQIVPKHQLLYTLDIAAYKDASDAS
                     LPPAAERLAPSISLLQEELLGILTEQVEGIQFSPRIGATGPESSEVLITGEFLRPDEE
                     FPRTKGCNKIVLTLRLDGGEVLWQENTEKHRDKGCGAF"
     gene            complement(3329..>3894)
                     /locus_tag="VFH91_10925"
     CDS             complement(3329..>3894)
                     /locus_tag="VFH91_10925"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET6863555.1"
                     /translation="DLGYFGEFGVRGVGYYVEDLREHITKILGLDSPHRVGIVGAGRL
                     GTALANYNGFGRSNFAVAAIFDNDSQKIGTTIGDIKLEVQDVERLGQVIRDEAIDVMV
                     IAVPAKAAQRVLNQIMSAGIKAVLNFAPVSLNSRRGVKVKTVDLTTSFESLSYFLARP
                     QQPTAMTNLGNKPTRKENSEQEEQRHV"
BASE COUNT          939 a         1100 c          873 g          982 t
ORIGIN      
        1 aacgcgcgaa gtacaaagtg caaagcacaa agttcgaagt actagtcctt tagaatcctc
       61 gcgaggaaca catcgcgcag tgatcgcatg gtttaccacc aggttcaact gtgcaagtct
      121 tctcgctgtg aaagaaatcc actagttcgg agacagcagc ctcttcgact ccgaatcgct
      181 cctgactggg atgagatgta ttgacacctg caagccacgg cgaatgaatc cgactactcg
      241 ctatttgatc ggactgacta gctgatgatg cggtgccgat cttggactcg atctgaatca
      301 gccggttttg tagttgcgac agattctctc tgatcgctgc cagttccgat gagacctcga
      361 agttcgagcg gggtacggcc ttaggaacag ccgcgtccgc agagatccgt cgcgcaattc
      421 tttctgccat cgaccgtgcc tgttcagaac tcatcttttt atatcttggt aattaatact
      481 atgagtagca agttaagcag caacagcatc ctcggtcagg tcagtaccgg atcgcggtag
      541 cgtcgggtaa gcctcacgaa caatcaactc ttagttactc gaggcttacc cgacgctact
      601 cgcgatccgg tactgacctc gcctggaatg ctcatcccaa caaatctacc cgatcgacaa
      661 ctcctactat cgccgaatca atcgccgcac cagcttcctt cgtcgcagcc gttgaagcac
      721 cccaaccctc ttgcacaatt agcactgtat ctccgacacc ggagtcgact gagtctaacg
      781 cgatcacagt ctgattcatc tcctcacctt ccggcgttaa ctgctggcac aacatgatac
      841 gcgcactctc gtagcgtcga ttcttcttag tggcaaccac gtttcctaca acccttgcaa
      901 gaatcatcgc tcttccttag ctaacgagta acatgtgcgt cagagtcgac tattccgacg
      961 atggtgcaat cagttggagt atcttcacgt ttgaatggga aacttgcttc cttgccgcga
     1021 caccaaaaca caagttctcc gacaccagct cctaccgaat cgactgcgac catcggcttt
     1081 ccgactggct gaagattctt atcgagcgtt tgcacaacca tcagttttcg cccgtcaagt
     1141 gactcgttct ttacagttgc gactactgtt ccgataaccc gagcaagttg cattcgctta
     1201 agagttcgtc acatcaaccg tatcgatgac tccaacaatc gcagcatcga ccggaacatc
     1261 tttcatgccc tgggccagac gagcgctcga tcctgctacg accaacactc gttcgtggaa
     1321 tccagcgccg actgtatcga ccgcgacaac ataaccagtt gtgtcacttc cgtcgacatt
     1381 gaccggacgc acaatcagca gcttcttgcc ggtgagtcgt tcgtcctttt gcgttgagac
     1441 aactgtgccg aggatgcgtg cgatgatcat tccgagttat cgctctaaac caattggcag
     1501 cgtttcatca acacttccgt gcggacgagg aatgacatga acactcacca actcacctac
     1561 gcggcgcgct gctgcggctc cggcatccgt agccgcctta actgccgcca cgtctccacg
     1621 aacaattgca gtaacaaaac ctgcaccaat tttttcatag ccgacgagcg taacattcgc
     1681 agctttaacc attgcgtcgg ccgcttcaat catagcgacc aaacccttcg tttcaaccat
     1741 tccgagtgct tcctgcattc agatctctcc tgtacgggaa ttagaactaa cggcgtgaaa
     1801 attgtgcgat gactctaacg tttaaaccga aaacggtcaa atcaagtgat aattatcaat
     1861 tgacaattat gaattgttaa ttatcacttg ggtggatggc ctgactcagc aactcaacta
     1921 actcttcacg tgtcaaagaa atctttcctg agccgttcgt tgtctctgta tacgacgtgc
     1981 gacgagctgg acgatcgcca gtcggacaag tcagctgagt ctcatgcagg taaccgcagg
     2041 cctggcacct tgcactttca cccaggtagc cggaagcttc tcgcacgttt attaacttct
     2101 cgatcgcatc ggccggcaga ttttttgagc cgcctaagac acgcgccagg attgcgatct
     2161 tcgctgtgtg ctcaagagtt tcaagtctgt cccacgcttg ccagagatcg gaaccatacg
     2221 cgaccgctcc gtggtttgcc attaagagtg cgttgtgatg tttcacaagt ggctgcattg
     2281 cttcagtcaa ctcgttggtt gacggagttc catacgaagc aagtggcaca caaccaagag
     2341 ttaaaatgac ttctgaaaga attggttggt cgatcgcgag tccagcgact gcgaacgcag
     2401 ttccatgcgg cggatgagca tgacaaacag cttttacgtc aggtctttcg cgatagatca
     2461 aaaggtgcat cgcgagttca cttgaggctt tgcgatcgtt aagagacttt ccattcagat
     2521 cggtgacggc gatcaggtct tccgtcattc gacctttgca ggtcatcgtc ggagtggcaa
     2581 gtattcgacc atcgtcaaga cgcaagctca cattgccatc cgacgatacg acgtagcttc
     2641 gctcgtacat tagatgacag atctgaatca gatcccgtct cgctgtttgt tcgtcaatca
     2701 ttgtgaaagg gtgcgtggtc agaacgctcc gcagccttta tcgcgatgtt tttccgtatt
     2761 ctcttgccac aagacttcac caccgtctaa tcgcaaggtg agcacgatct tattgcaccc
     2821 tttagtacgg ggaaactctt cgtctggacg caaaaactct cccgtgatca acacttcgga
     2881 tgattctgga ccagtcgccc ctattcttgg actaaattgt ataccttcga cttgttcagt
     2941 caggataccc agcaattcct cttgtagcag cgagatactc ggagcgagtc tctcagcagc
     3001 gggtggtaac gacgcatccg aagcatcttt gtaagcggcg atgtctaacg tataaagaag
     3061 ctggtgctta ggaacaatct gctcaacttt gccattcgca tcaaacactg cccttaaact
     3121 cacggcggcc ctcttcatat ggagccgatt gagtgactcc ggcatctcag agtcgggcac
     3181 actccttatt accaacggct ggtccacctg tccaagcacg gcttttgttg tggcagtccg
     3241 attggtatga accaggtaag caataccaag tcccagaaaa aacgtcagta ggagcggtaa
     3301 cagacgtatc gccattcctc ttccaccttc acacatgtct ctgttcctcc tgttctgaat
     3361 tttccttccg ggttggcttg ttccccagat tcgtcattgc cgtcggctgc tgcggacgcg
     3421 ccaaaaaata cgatagcgat tcgaacgaag tagttaaatc aactgtctta actttaacgc
     3481 cgcgtcttga gtttaaagag accggagcga aattcaacac tgccttaatt ccggccgaca
     3541 tgatctgatt caacactctc tgcgcggctt tggcgggaac ggcaattacc atcacatcga
     3601 tagcttcatc ccgaatgacc tggcctaacc tctcaacgtc ctggacctct aatttaatgt
     3661 cgccaattgt tgtgccaatt ttttgagaat cgttatcgaa gattgccgct acagcaaaat
     3721 tagacctccc aaaaccattg taattcgcca gcgccgttcc cagccggccc gcccccacta
     3781 ttccaacgcg atgcgggctg tcgagcccaa ggattttcgt aatgtgctct cgcaaatctt
     3841 cgacgtaata accaacgcct ctgacgccaa actcgccgaa gtatcccagg tctt
//
LOCUS       DASSFP010000484         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_592809, whole genome shotgun sequence.
ACCESSION   DASSFP010000484 DASSFP010000000
VERSION     DASSFP010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592809"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFH91_10930"
     CDS             <1..322
                     /locus_tag="VFH91_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6863556.1"
                     /translation="DPEIPVSCEEIFGPVATIFKVETLDQAIRIANITNFGLGSAAWT
                     SDPIEQKEFVERLEAGSVFINGMVASDPRLPFGGVKNSGYGRELSEFGIREFVNIKTV
                     WIGE"
     gene            394..789
                     /locus_tag="VFH91_10935"
     CDS             394..789
                     /locus_tag="VFH91_10935"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HET6863557.1"
                     /translation="MTSKLSKLATLLLMATILGLTVSCRNQTPSQVKSQPSPTPPNIK
                     PMTFPTAVVGKPYPGVGVVLLINKKEGWIEINHDEIVDLMPPMVMEWQVKDRALLNRV
                     KVGDKVNFVVVETGSGEIITDLNKIPEQK"
     gene            796..1635
                     /locus_tag="VFH91_10940"
     CDS             796..1635
                     /locus_tag="VFH91_10940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6863558.1"
                     /translation="MAATNTDSYPFVDIDLARRLEQTEAMGNVKFIEARIKAFPDRGA
                     TWRRIGGTFAMFDAVGSPLTQTFGLGMFQPVTNDDMDQLERFFHDRGSDVFHEVSPLA
                     DPSAMDLLNERRYRPIELTSVMFRPITTDLRLDATRNDLIAVRLINDDEEDLWAQTAA
                     DGWNEYPELRDFLLELGKVNAQSESLSFIAELKGKAIAAGALTINDDVALLAGASTIP
                     EFRRNGAQLALLEDRLRYGAGHGCNTAMIAARPGSASQRNAERHGFRIAYTRIKWELR
                     SAA"
     gene            complement(1641..2177)
                     /locus_tag="VFH91_10945"
     CDS             complement(1641..2177)
                     /locus_tag="VFH91_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6863559.1"
                     /translation="MTRSSRIVVGSSLPVLILVPLIVVTGFTISSSQNANSSTTNKNQ
                     NDTRAITPPARGGQQQDFSKNTWELPDDAEKTKNPVASTPESIAKGKELYLNKDKGNC
                     AFCHGETGAGNEANLPNLRRKPADISSKERMSVMTDGEVFWKISRGIRGIMPAGEKRM
                     NEEERWNVVNYIRTLPKE"
     gene            complement(2217..3329)
                     /locus_tag="VFH91_10950"
     CDS             complement(2217..3329)
                     /locus_tag="VFH91_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863560.1"
                     /translation="MHNNSCKRLLFLVTLLVILVQAASAQKAYVKSVPVQSVVNVLST
                     LPEADVLIYTSPQKILNEAAPKVIDATEIAKMRSTFDDLKKSVGIDPSTIDYLVIAVR
                     FHKPAADLSFVAPDVMTVFGGDFSSASLFETGKLYLQEKLRDETYGSKTISIMPIDSI
                     AEAAVKNPLLKSFVEVGAVPLSANSIAVGNVRYLKAAIDAVDGKGQRLQSATLDSLLR
                     DQTALISAAGSPLTSFMKSFGMLGTETTAREARCDTRFGDFYGAVTLSGTNFSVRGAM
                     NTDNPDTAKIINNLVSGLWQQAIDSVPDKNAQSMLKAIRIIPRESELVWEADISDQTV
                     ANFIKEQTKPKVTAPPETSKPAPAPRQKPRRRKPRG"
     gene            complement(3401..>3827)
                     /locus_tag="VFH91_10955"
     CDS             complement(3401..>3827)
                     /locus_tag="VFH91_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6863561.1"
                     /translation="NLIRRCNRFSELAWKTIMEKVNLQQKFSTFTEHWDPRIAGELNG
                     QYIKLVKFQGPFTWHHHENEDELFMVVRGRFRMDYRDESNSDTSTWIEEGEFIVVPRG
                     VEHRPFAEAECHVLLFEPVSTLNTGDVQNEFTKRELRAI"
BASE COUNT          944 a          927 c          936 g         1020 t
ORIGIN      
        1 cgaccccgaa ataccagtat cctgcgaaga gatttttggc ccagtagcaa cgatcttcaa
       61 agttgagacc ttagaccagg caatcagaat tgcgaatatc acgaactttg gtttaggctc
      121 ggctgcatgg accagcgatc cgatcgagca gaaagaattt gtggaacgtc tcgaggcagg
      181 atcggtgttt ataaacggaa tggttgcatc cgatccgcgg ctaccattcg ggggagtcaa
      241 gaattccggc tatggacgtg aactctcgga gtttggcatt agagaattcg taaacattaa
      301 gacggtctgg attggcgaat aattgaggcg tgaggctttg ttctttgatc tttggtcaat
      361 atcaattgtt ggtgcgcgca tggatgactg gaaatgacat caaaacttag caagcttgcg
      421 actcttctgc tgatggccac catattgggt ttgactgtgt cgtgccgcaa ccaaacaccg
      481 tcgcaagtta aatctcagcc atcacctacc ccaccaaaca ttaagcctat gacttttcct
      541 acggctgttg tcggcaagcc ttatcctggc gtcggagtag tgctcttaat caataaaaag
      601 gaggggtgga ttgagatcaa ccatgatgag attgttgact tgatgcctcc tatggtgatg
      661 gagtggcagg taaaggaccg tgccttactc aaccgggtca aggtgggtga taaggttaac
      721 tttgttgtcg tcgaaaccgg tagcggcgag atcatcactg acttgaacaa aatcccagaa
      781 cagaagtgaa tattaatggc agcgactaac acagactcgt atcccttcgt cgatatcgat
      841 ctcgcacggc gtctggaaca aactgaagca atgggcaacg tgaagtttat cgaagcccgc
      901 ataaaggcat ttccggatcg aggagcaacc tggcgccgaa taggcggaac gtttgccatg
      961 tttgatgcag tgggttctcc gctgactcaa acgtttgggc tcggaatgtt tcaacctgtt
     1021 accaacgacg atatggacca gttagagcgt ttctttcacg accgaggctc agacgtattc
     1081 catgaagtgt cgccattggc tgatccgtca gctatggatt tactcaacga acgccgctac
     1141 cggccaattg aattgaccag tgtaatgttt cgtccaatca cgactgactt gcgtttagat
     1201 gctactcgga acgatctaat agcggtgcga ttaataaacg acgatgagga agacctctgg
     1261 gctcaaacgg cggcagatgg ttggaatgaa tatccagagc taagggattt tttgctcgaa
     1321 cttggtaaag tcaacgctca atccgagtct ctttcattca tagccgaatt aaaaggtaag
     1381 gcgattgcgg cgggcgcgct aactatcaat gacgatgtgg ctcttttggc cggggccagt
     1441 acgattccag aattccgtcg aaatggagca cagctggcat tacttgagga tcgtttgcga
     1501 tatggagcag ggcatggttg taataccgca atgattgcgg cgcgtcctgg gagtgcgtca
     1561 caacgcaacg ctgaacgcca tggatttcgc atagcctata cgcggataaa gtgggaactg
     1621 cgaagtgctg cttgagttta ttattctttc ggtagcgtgc ggatataatt cacgacattc
     1681 cagcgctctt cttcgttcat ccgtttctca ccagcaggca tgatgccgcg aatgcccctg
     1741 gaaatcttcc agaatacttc gccgtccgtc atcactgaca ttcgttcttt acttgagata
     1801 tctgccggct tccgtcgcaa gttgggcaag ttcgcttcgt ttcccgcgcc tgtctcacca
     1861 tgacagaaag cacagttacc tttgtctttg ttcaggtaaa gctctttgcc tttggctatc
     1921 gattccggag tcgacgctac gggattcttt gtcttctcag cgtcatcagg aagttcccag
     1981 gtgttttttg aaaagtcctg ctgctgacca ccccgcgcag gaggggttat tgctcgcgtg
     2041 tcgttctgat tcttattcgt ggttgaacta tttgcatttt gtgaggacga aatcgtaaat
     2101 cccgtcacca caatcagcgg caccaggatt aagaccggca atgatgaacc cacaactatt
     2161 cgagatgatc tagtcactac agctgtcctc cttggagtgt ttttctactc agctatttac
     2221 cctcttggct tgcgtcgtct aggtttctga cgtggagccg gcgcaggctt actcgtctca
     2281 ggtggcgccg ttaccttagg cttggtctgc tccttaataa agttcgccac tgtttgatcg
     2341 gaaatatctg cttcccaaac gagctcactc tctcgaggaa tgatccgaat cgccttaagc
     2401 attgactgcg cattcttgtc gggcactgag tcgatcgctt gttgccataa accggaaacc
     2461 aaattgttta tgattttggc ggtatctgga ttatcagtgt tcatcgcacc tcgcacactg
     2521 aaatttgttc cactcaacgt taccgctcca taaaaatcac cgaagcgtgt atcgcaacgt
     2581 gcctcgcgag cagtagtttc agttccaagc atgccaaaac ttttcataaa cgaggttaaa
     2641 ggcgaaccgg cggcactgat gagagcagtt tgatcgcgca gtaacgagtc taacgttgct
     2701 gattgcaacc gctgtccttt accgtcaaca gcatcgatcg ccgctttcaa gtacctcacg
     2761 ttaccgacgg caatggaatt ggcactcaaa ggtactgcgc ccacctcgac aaatgatttg
     2821 agcaacggat tttttacggc ggcttccgca attgaatcta tcggcatgat tgaaatcgtt
     2881 ttcgatccat aggtttcgtc ccgcagtttc tcctgaagat acagctttcc tgtttcaaat
     2941 aatgatgcgg agctgaagtc gccgccaaaa acggtcatca catcgggagc gacgaagctg
     3001 agatctgctg cgggcttgtg gaacctgacc gcaatgacga gatagtcgat cgtcgaagga
     3061 tcaataccga cagatttctt tagatcgtca aatgtcgaac gcatttttgc gatttcggtc
     3121 gcatcaatga ctttaggtgc agcctcgttc aagatctttt gtggactggt ataaataagt
     3181 acgtctgctt ccggaagtgt cgaaaggaca ttcaccacgg actgaaccgg aacagattta
     3241 acgtaagctt tttgagccga tgccgcctgg accaagatca ccaacagggt gactagaaac
     3301 agtaagcgtt tgcaggagtt gttgtgcaaa ttaaacctcc actttggagt gcggcggccc
     3361 gcgccgcttt gttataggtt caactacaag tccactctgc ttagatcgct ctaagctctc
     3421 tcttcgtaaa ttcgttctgc acgtcgccag tattcagcgt agaaactggt tcaaacagga
     3481 gcacatgaca ctctgcttca gcgaagggac gatgttcgac accacgggga acgacaatga
     3541 actcgccttc ttcaatccac gttgaagtat cggaattgct ctcatccctg tagtccattc
     3601 tgaaacgccc gcgaacaacc atgaacaact catcttcatt ttcatgatga tgccaggtga
     3661 atggcccctg gaacttaacc agtttgatgt actggccatt cagttcgccg gcaatacgcg
     3721 gatcccaatg ttcggtaaag gtcgaaaact tttgctgtag atttactttc tccatgatcg
     3781 ttttccatgc tagttcagaa aacctattgc aacgacggat cagattc
//
LOCUS       DASSFP010000485         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1786855, whole genome shotgun sequence.
ACCESSION   DASSFP010000485 DASSFP010000000
VERSION     DASSFP010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786855"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2200)
                     /locus_tag="VFH91_10960"
     CDS             complement(<1..2200)
                     /locus_tag="VFH91_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6863562.1"
                     /translation="MHGVDFSTGLESNSILIVNDFPEQLKLMGRLLRNAGYTVLTAED
                     GFEAFEIAKDQQPNLIISDVSMPRMNGLEFCAKVRETRELRSVPILLISALQKDTETI
                     VAGLRAGADDYLEVPFDSARLIAKVARLLERSRIESSYRDLVEQANDVIFTQDMAGKL
                     TGINAAGARFLGRAPGDIVGQSFFSAFGIIPESNGFGTNLNGSEAPAEFRHQFVARSA
                     SGEDRWLDLSISPIRDKFEEPVGFRGLARDVTDRRRFEEALRDSEERYRLLFETTPQP
                     IWVYNEDTFTFVAVNEAATRTYGYSREDFLSMTVHDLRPQEDIPTLLIKSEIGPDELV
                     ISSPWRHQTRDKKIIYVEMSSHPVVFDGKNSKLVIVNDVTEKKLLDEKQQKLHTSLQQ
                     SAMEWRQTFNAIDFPVLIVDLGGVIRRANEAAEEIVGAEADQIIGKAISEFSDAEPWK
                     TAAALIEVIKKEQSPVSEEVKDQINKKTWAITLYLINEFGSVGERAILIAQDITKRTE
                     LEASLRQSEMMSLLGSLVAGVAHEVRNPLFGISSILDAFEKRFSDRTEYLRYTNVLRD
                     EIGRLTVLMEELLEYGKPFRRDFYLVPVAEMIARSIKACLPAADLAHVTLEADVAESL
                     PKLRIDRRRLLKVFVNLIENAIHHSPPGGVVMLEAKLVNEGEQDWIECTIKDVGPGIL
                     ETDMSRIFEPFFSKRRGGTGLGLAIAHRIMDEHGGQLFAGNNPDGGACMTT"
BASE COUNT          572 a          667 c          555 g          590 t
ORIGIN      
        1 ttgtagtcat gcaggctccg ccatcaggat tattgcctgc aaagagctgt ccgccgtgtt
       61 cgtccataat ccgatgggca attgccaatc ccaatcctgt cccaccgcgg cgctttgaaa
      121 agaaaggttc aaaaattctc gacatatccg tttccagaat gcctgggccg acatccttga
      181 tagtgcactc gatccaatcc tgctctcctt cgttcaccag ttttgcctcc aacatcacca
      241 ctcccccggg cggcgaatga tggatagcgt tctcgatcaa gttaacgaat actttcaata
      301 gccggcgccg gtcaattcgg agctttggca acgattctgc cacgtccgcc tcaagtgtta
      361 cgtgcgccag gtccgcagcc ggcaaacatg ctttgatcga gcgagcgatc atttccgcca
      421 caggcacaag atagaagtct cgacggaatg gctttccata ctccagtaac tcttccatta
      481 acacagtcaa ccggccgatt tcatcccgca gaacattggt ataacgtaaa tactccgtgc
      541 ggtcgctgaa acgcttttca aaagcgtcga ggatcgagga gataccgaag agtggatttc
      601 ggacctcatg tgcgacgcca gccaccaggg acccgagcaa cgacatcatc tcactctgtc
      661 gcagcgacgc ctcgagttcc gtccttttgg taatatcctg agcaattagg atggcgcgct
      721 cgccaaccga tccaaactca ttaattaggt aaagtgtgat tgcccaggtt ttcttattta
      781 tctgatcttt gacctcttcg gaaactggcg actgttcctt cttgattact tcgatcagcg
      841 cagcggctgt tttccagggc tctgcatcgg agaattcaga gatcgcttta cctatgattt
      901 ggtccgcttc ggcccctaca atttcttcgg ctgcctcgtt tgcacgccgg ataaccccac
      961 caaggtcaac tatcaaaact gggaaatcga tggcattgaa agtctgcctc cactccatag
     1021 cagactgttg cagcgacgtg tgcaacttct gctgcttctc gtccagcagt ttcttctctg
     1081 taacgtcatt aacgatgaca agcttgctgt tcttcccatc gaagacgaca gggtgcgaac
     1141 tcatttcgac gtagataatt ttcttatcac gtgtttgatg gcgccacgga ctcgagatca
     1201 cgagctcatc cggccctatt tcactcttta ttagaagggt cggaatatct tcctggggcc
     1261 ttaaatcatg cacggtcatc gacaagaaat cttctcgcga gtagccgtaa gttcgcgtcg
     1321 cagcctcatt gacggccacg aacgtaaagg tatcttcgtt atagacccag ataggttgcg
     1381 gcgtggtttc aaaaagcagt cgatatcgtt cttcagaatc gcgcaaggct tcctcaaaac
     1441 gtctcctgtc ggtaacgtca cgagccaacc cccgaaaccc aactggttct tcgaacttgt
     1501 cgcgaatggg cgagatacta agatcgagcc aacggtcctc acctgaggcg gaccgggcca
     1561 caaactggtg ccgaaattct gcgggcgctt cagaaccgtt cagattggtc ccgaaaccgt
     1621 tgctttccgg aataatgcca aaagccgaaa agaatgattg cccgacaatg tcacccggag
     1681 cgcgacccag gaacctcgcg ccagcagcat taataccggt cagcttgcca gccatatcct
     1741 gggtaaaaat cacgtcgttg gcctgctcaa ctaaatccct ataactggat tcaattcgcg
     1801 aacgttcaag taagcgagcc accttcgcaa tgagccgcgc ggagtcgaag ggtacttcca
     1861 aatagtcgtc agcccccgcc cgcaaaccgg cgacgattgt ctcggtgtct ttctgaagag
     1921 cactaataag gagtattgga acactcctta attccctggt ttcacgcacc ttggcacaga
     1981 attccaggcc gttcatccgc ggcattgaaa catcgctgat tatgaggttg ggttgctgat
     2041 ccttggcgat ctcgaaggct tcaaacccat cttccgcggt cagaaccgtg tatccggcgt
     2101 ttcgcaacaa acgacccatg agcttgagtt gctcggggaa gtcgttgacg atcagaatag
     2161 aattgctttc aagaccagta gagaaatcta ctccgtgcat ttacctcata cctcgatcta
     2221 ctacctttgc tggaacttgc tggcgaagcg tctctcggcc gcgggattgg atgtattttg
     2281 atccccgttc ggaataagaa aatcagggat ttatcgggtt ttcaccggcg gagtatagca
     2341 cacgaaatcg atcctaaaga ccgcaaaagc cctgtcttca tctc
//
LOCUS       DASSFP010000486         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1476416, whole genome shotgun sequence.
ACCESSION   DASSFP010000486 DASSFP010000000
VERSION     DASSFP010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476416"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..2879)
                     /gene="topA"
                     /locus_tag="VFH91_10965"
     CDS             complement(348..2879)
                     /gene="topA"
                     /locus_tag="VFH91_10965"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET6863563.1"
                     /translation="MAKNLVIVESPAKAKTINKYLGKDYLVKASIGHIKDLPSKGLGV
                     DVEHDFKPTYELIPDSKKRNNKRIVAELKKAAKEADAVYLAADPDREGEAICQHLAEE
                     IVPKKPKKPAYRVMFNEITKRAVNEAFERPRQIDENLVDAQQARRVLDRLVGYKVSPL
                     LCRTIGGRLSAGRVQSVALRMVVEREREIEQFIKTEYWTIAANLGAKIPPAFDSRLFK
                     IEEQTVKTSGFDQDIKKNEVLINNEKSAEDIVAEAKDQRFVVSEVTTKERKRNPVPPF
                     ITSKLQQEASRKLGFAVKKTMMLAQKLYEGIELGAEGSVGLITYMRTDSTRVSEAALA
                     EVRDFIGKQYGDRYLPEKAVHYRSKKDAQDAHEAIRPTEVVRTPESLAQYLNQEELKL
                     YRLIWQRFVASQMTAAIFDQTTIDIQAGRFLFRATGSVQKFDGFLKLYQEGRDEKADD
                     DDEAELTLPLVEKGETLVLNSINPEQHFTEPPPRYTEATLVKALEEKGIGRPSTYAAI
                     MSTIQEREYVEKLQGRFHPTGLGKTVNDLLIDGGFDELFNESYTAKMEEELDEIEEGK
                     IEWTKALRDFYKKFEGDLDKFKVYVKDIKEKNVPTEEVCLKCNTPGMVQKWGRFGSYL
                     KCLSCDATRDAEPPPGENGANGAEAASQSAEETEPETCELCGKPMQMKRGRFGPFLGC
                     TGYPECRNIRKIGKSGVAAPPPVPLDEKCPVDGAQLVKRFGRFGEFISCSNYPKCKYI
                     KQETVGVSCPRPGCKGEIVVKKSKRGKAFYGCSEYPNCNIVYWDKPVSETCPQCNAPF
                     LLEKTTKKQGTFRYCANEECGYKSDAAAETIVKTEPSRPGSSVST"
     gene            complement(2945..>3475)
                     /locus_tag="VFH91_10970"
     CDS             complement(2945..>3475)
                     /locus_tag="VFH91_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET6863564.1"
                     /translation="GTPPVSENFPYRNRIISGLSLGVLVVEASENSGSLITARLAMEQ
                     NREVFAVPGNITSRNSFGTNYLIKGAGAKLIQQWQDVATELPPQIAASLLPPPFSDRT
                     KELSLANRLSFVPEGLSTTETSVFKLLSPDSPVHIDSLIDSSRLRMPDLTAALLSLEM
                     RELIRALPGKCFVRKL"
BASE COUNT          775 a          925 c          784 g          991 t
ORIGIN      
        1 agcaacgctg tatggtcatc gtagttctcc tcgaattgta ggttttgccg cgcatacttc
       61 agtatgcaca gccttaacga ttggatagga cgcctaatac tgcacctgct tgagaaaggt
      121 tgcaagcgca gcactgttca cttgaatgga ttaacggcta attatttgga tgagaggctt
      181 ggattatttg agaattggga tttgatattt gagaactgct atctgtgccg cagtacaatt
      241 cccaatacta agagacttcc agaatctact aagagacttt cagaatctca aacctcgccg
      301 ctcaccacct aataattcat aattctcaaa tgataattat caaatactta ggtgctcact
      361 gagctgcccg gtcgtgacgg ttccgttttc acgattgttt ccgcagctgc atctgacttg
      421 tagccgcact cttcgttcgc gcaataacga aacgttccct gcttcttagt agttttttcg
      481 agcagaaagg gcgcgttgca ttgcgggcag gtttcgctta caggcttgtc ccaataaacg
      541 atgttgcagt ttggatactc agaacacccg taaaatgcct tacctcgttt tgacttctta
      601 acaacgatct cacctttaca gccaggccga ggacatgaaa caccaactgt ctcttgtttg
      661 atgtacttgc atttggggta gttcgaacac gaaataaatt cgccgaaacg cccgaagcgc
      721 ttgaccagct gcgcgccgtc aacgggacat ttctcatcca ggggaaccgg tggcggagcc
      781 gccactccac tctttccaat ctttctgatg tttcggcatt ctggataacc ggtgcaacca
      841 aggaatggac caaaacggcc gcgtttcatc tgcatcggct tgccacacaa ctcacaagtc
      901 tccggctcgg tctcctcggc tgattgtgag gctgcttctg ctccgttggc gccattttcg
      961 cccggtggtg gctctgcatc acgagtggcg tcgcagctca aacatttcaa gtacgaaccg
     1021 aagcgtcccc acttctggac catccctggc gtattacatt tcagacaaac ttcctcagtc
     1081 ggaacgttct tctcttttat gtcctttaca tagaccttga acttgtcgag atcaccttca
     1141 aacttcttat agaagtcgcg cagagccttg gtccattcga tcttgccttc ctcgatctcg
     1201 tccagctctt cttccatttt cgccgtataa ctctcgttga agagctcgtc gaacccaccg
     1261 tcgatcaaca gatcgttaac tgtctttccc agcccagttg gatgaaaacg tccttggagt
     1321 ttttctacgt attcgcgctc ctggatggtc gacatgatcg ccgcataagt cgatggcctg
     1381 ccgatcccct tttcttctaa agctttcact agagttgctt cagtgtatcg gggtggtggt
     1441 tcggtgaagt gttgttccgg attgatggag ttaagaacca gcgtttcgcc tttttcgact
     1501 aaaggcaaag taagctctgc ttcgtcgtcg tcatcggcct tttcgtctcg tccttcctga
     1561 tagagcttca ggaatccatc aaatttctgg accgacccgg tcgcgcggaa tagaaaacgg
     1621 ccagcctgaa tatctattgt cgtctgatcg aagatggcgg cagtcatttg cgaagctacg
     1681 aaacgttgcc agatcagacg atagagtttt agctcttcct gatttaaata ctgagccaac
     1741 gactcggggg ttcgaaccac ttcagtagga cgaatggcct cgtgagcatc ctgcgcgtct
     1801 ttcttcgagc ggtaatgcac tgctttctct ggcaaataac gatcgccgta ctgcttaccg
     1861 ataaagtcac gtacttcagc aagcgccgct tcagagactc gcgtcgagtc ggtacgcata
     1921 taagtgatca gaccaacaga tccttcggcc cctaactcaa ttccctcgta cagcttttgg
     1981 gccaacatca ttgttttctt aactgcgaag cccaacttcc gcgaggcttc ctgctgcagc
     2041 ttagaggtga tgaatggtgg tacgggattc cgtttgcgct ccttcgtagt aacttctgaa
     2101 acaacgaacc tttggtcctt cgcttccgca acgatgtctt ccgcgctctt ttcgttgttg
     2161 atcagtactt cattcttctt tatgtcctga tcaaacccac ttgtctttac tgtctgctcc
     2221 tcgatcttga acagtctgga atcaaacgcg ggtggaattt tcgcaccaag attggccgcg
     2281 atggtccagt actcagtttt tataaactgt tcgatctctc gttcgcgttc aacgaccatg
     2341 cgcagcgcta ctgactggac acgacctgcg ctcaaacgtc cgccgattgt gcgacacagc
     2401 aacggagaca ctttgtatcc caccaatcgg tccaacacgc gtcttgcctg ttgagcgtca
     2461 acgaggtttt cgtcgatctg ccgtggacgc tcgaatgctt cgttaacggc gcgtttcgtg
     2521 atctcgttga acatcacccg gtacgcaggt ttcttaggtt tttttggaac tatctcttca
     2581 gctagatgct ggcagatagc ttcgccttcg cggtcggggt cagctgcgag gtacaccgcg
     2641 tcggcttctt tagccgcttt tttgagttcc gctacaatcc ttttgttatt gcgcttcttt
     2701 gaatccggaa tcagttcata cgtgggcttg aaatcatgct caacgtcaac tcctaatccc
     2761 ttcgaaggca gatctttgat gtggcctatc gaggccttaa ccagatagtc ctttcctaaa
     2821 tatttattta tggttttcgc ctttgccggc gattccacga ttactaaatt ctttgccatt
     2881 agttctcttt taactccata ccagcactct gtgctttgcc tgggacattg tgcatttaac
     2941 tgggttacaa tttcctgacg aagcactttc ctggtaatgc tctgatcagc tccctcattt
     3001 ccaacgacaa taacgcagcc gtcaagtccg gcattcgaag ccggctacta tcgattaggg
     3061 aatcgatatg taccggactg tcaggactca acagcttaaa gaccgaagtc tcggttgtag
     3121 aaagtccctc tggaacgaat gacagccggt tggccaacga caattccttt gtccgatcac
     3181 taaaaggtgg cggcaacaaa ctggctgcaa tttgcggcgg caattcggtg gcaacatctt
     3241 gccactgctg gattaacttg gctccggctc ccttaatcaa atagtttgtg ccgaaagagt
     3301 ttcgagaagt tatgttgccg ggaacagcaa atacctcacg attttgttcc attgccagtc
     3361 gcgctgttat caaagatccc gaattctcgg acgcttccac taccagaact ccgagactca
     3421 gcccgctaat gatgcggttt cggtacggaa agttttccga caccggagga gtacc
//
LOCUS       DASSFP010000487         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1189821, whole genome shotgun sequence.
ACCESSION   DASSFP010000487 DASSFP010000000
VERSION     DASSFP010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189821"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFH91_10975"
     CDS             complement(<1..699)
                     /locus_tag="VFH91_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6863565.1"
                     /translation="MAQKESTQTSVNLARRLGLFDATMIVMGGIIGSGIFMNPSVVAI
                     QVHTSFLILGAWVVGGIVALIASFIWAELAAVRPEVGGQYAYLREAFHPGVAFVYGWV
                     LLLVIQTGGMAAVAVTFSRYFVELTGLALNYSIVAATVLCALTLINCLGVRAGSTVQS
                     ILMVLKILAIIMLVGCGLLLARSIPGSTPLLDQPPSLGLLTAFGAAMVPVLFSYGGWQ
                     TATFVAGEIKEPSKN"
     gene            1014..2726
                     /locus_tag="VFH91_10980"
     CDS             1014..2726
                     /locus_tag="VFH91_10980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6863566.1"
                     /translation="MSVRAEAIITAGLRPDLFASQNTLSSSRTLIFAALAALIIAGFG
                     FRITGLGAEGLGEDELNKLNAVADYRAHGLTGANGEHPMLMKALQAASIILIEKWNES
                     SFVANTPADQISPETALRLPGVIAGAFTSLLIYLITAELFGAEVALIAAALWAFDPTA
                     IGFNRVAKEDTFLLFFFLLANVFWLRGQRIAESTNQSPNRYYWLSAASFGAMVASKYV
                     PHLFGISMAYYWMFQNLPETKWRLGKVRLLTMFAIMGLVFLILSPTILLPETWRQMGL
                     FAGSKRVSHDGYEFMGQLYTQGFSDWLRGIPVYFYLVFTWVKLPVLTVAGFLIGLPLL
                     FRRKLGDGRYFILFWMFLWFISFGFSGGKFTRYYTTVLPAVLITSALGIQYAGRWLSQ
                     RFSGSWSNSVAQYLPATLAVLVITCSVVSSAQVSPHYRLFTNVLGGGMNSAGYYFPHD
                     EFYDASMRDTIAEIAKRANPGAKVASESALVASYYAQRVSRPDLVALSLSDVENLKKL
                     GPGDFIIVARGRRYFSNDALISSLSKLKKPELEMFLGSVHSADVYVVDDEIFGVRRPG
                     AAFQ"
     gene            complement(2843..>3228)
                     /locus_tag="VFH91_10985"
     CDS             complement(2843..>3228)
                     /locus_tag="VFH91_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863567.1"
                     /translation="GSGFLEYTIPERADRRRVSEIIVRAHIQPVSPIDATRDFVRTRV
                     TLYINGWDCGSRLVPFEAKGQTLVQEWKATGLFLRLRAMRGLPLSIRFAVEPESDWLY
                     GVNVSNWPEGYDAKDAKPIEVELRH"
BASE COUNT          756 a          838 c          804 g          830 t
ORIGIN      
        1 attcttcgat ggctctttga tctcgcctgc aacaaaagtc gctgtttgcc agccgccgta
       61 agagaaaagc acggggacca ttgctgctcc aaaagcagtc agcagtccaa gggaaggtgg
      121 ttggtccaat agaggagttg agcctggaat gcttctagcc aacaggaggc cgcatcctac
      181 cagcattatg atcgccagga tcttcagcac catcaaaata ctttggaccg tgctgccggc
      241 tcgcacacct agacaattaa taagggtcaa ggcacataag actgtggctg cgacaatcga
      301 atagttcaga gcaagtcccg tcaactctac aaagtaccgc gagaaggtta cggctacagc
      361 ggccatacca ccggtttgaa taacgagtag caacacccag ccatatacga acgcaacgcc
      421 aggatgaaaa gcttcgcgca gataagcgta ctggccgcca acttcgggcc tgactgcggc
      481 gagttcggcc caaatgaaac tggcgatcag ggcgactatc ccgccaacca cccacgcgcc
      541 caaaatcaga aaggacgtat ggacctgaat ggcaacgacg gacgggttca taaagattcc
      601 ggatccgatg atgccgccca tcacgatcat tgtggcgtcg aagagtccga gccttcgcgc
      661 gagatttacg ctggtttgtg ttgattcttt ctgggccaca attattgagg acgcgctcgc
      721 gtttcgtgtt tcgtccctac cgtgtgagtt tgctcaagtc cgcccgctaa cattgccgct
      781 tggatagtga tgcagtttgg gatgtccgat atgctttagc ttgtcgtgtt cgcgttttgg
      841 cgattcgatt ctaaaatgaa ccttattgca caagagaccg acaagctaat aaagcatatc
      901 ggacatccca aactgcatca ctattgccgc ttgagcgtct ttgcactacc gtaagatttt
      961 cgctaccctt tcgacttttg ttgtaagcgt tgaattagta aggaatttat acattgtcag
     1021 ttcgtgccga agccattatc actgctggtc tgagacccga tttattcgcc agccaaaata
     1081 cactatcgtc cagtagaaca cttatctttg ctgcgttggc ggcactcatc atcgccggct
     1141 ttggttttcg tataaccggg ctcggcgccg aaggacttgg cgaggacgag ctcaacaaac
     1201 tcaatgccgt cgccgactat cgagcacatg gtctaacggg agctaatggc gagcacccaa
     1261 tgctcatgaa agcgttacaa gctgcgagca tcattctcat cgaaaaatgg aatgaaagtt
     1321 ctttcgtagc taatacaccg gccgaccaga tctcgcctga gactgccttg cggctgccag
     1381 gtgtcattgc gggagctttt acatcgctgc tcatttactt aatcaccgcg gaactattcg
     1441 gcgctgaagt ggctctgatt gccgccgccc tatgggcttt cgatccaacc gcaattggct
     1501 tcaaccgagt cgccaaagaa gataccttct tgctcttctt tttcctactg gcaaatgttt
     1561 tctggctgag aggccaacga atcgccgaga gcacaaacca gagtccgaat cgttattact
     1621 ggttgtcggc agcctctttc ggcgcgatgg tcgcctcgaa atacgttcca catctattcg
     1681 ggattagcat ggcctactac tggatgtttc aaaatcttcc cgaaactaaa tggcgcttgg
     1741 gcaaagtcag gctccttaca atgttcgcca tcatgggtct ggtgttcttg attcttagtc
     1801 caaccatctt attgcctgag acctggcgac aaatgggact cttcgccggt agcaaacgag
     1861 tgagtcatga tgggtacgag tttatgggcc agctttacac tcagggattt agtgactggc
     1921 ttcggggaat tccagtctat ttttatctcg tgtttacgtg ggtaaaactt ccagtattga
     1981 cggttgccgg gttcctgatc ggactaccgc tactatttcg acgaaagctc ggcgacggtc
     2041 gttacttcat actgttttgg atgtttcttt ggtttattag cttcgggttt agcggcggaa
     2101 agttcactcg gtattacacc actgtgctgc cggccgtact cattacctca gccctgggaa
     2161 ttcaatatgc gggacgatgg ctgtcacaac gattttctgg tagttggtcc aattcggtgg
     2221 cccagtattt accggcaaca ctggccgtgc tggtaatcac ctgctctgta gtcagttccg
     2281 ctcaggtatc accacactac cggttgttta cgaatgtgct cggcggagga atgaattcgg
     2341 cgggctatta ttttccgcat gatgagttct acgatgcgag tatgcgcgac acgatcgcgg
     2401 agattgcaaa gcgcgcgaat ccaggcgcaa aggtcgctag cgaaagtgcc ttggtggcct
     2461 cctactacgc tcaacgagtc agtcgtccgg accttgtcgc cttgtcgctt tcggatgttg
     2521 agaatctaaa gaagcttggt ccaggcgact tcattattgt tgcgagagga cgacggtact
     2581 tcagcaatga tgcactgatc tcctcgctgt caaaattaaa gaagccagag cttgagatgt
     2641 tcctgggaag tgttcattca gccgatgtct acgtcgttga cgacgagatt tttggagtgc
     2701 ggcggcccgg cgccgctttt cagtaacctg atttcccagg ctaacttgat taaacatagg
     2761 ctcttttaac ccagatcttt ttaatccaga tctcacaaac taatcttgag tgaaaagcgg
     2821 cgccgggccg ccgcactcca aattagtgac gcagttcgac ttcgattggt ttggcatctt
     2881 tcgcgtcata tccttcaggc caattggata cgttcacgcc ataaagccaa tctgattccg
     2941 gttcaaccgc aaaacgtatc gagagtggca gacctctcat agcgcgcaat cgtaagaaca
     3001 accctgtcgc tttccattct tgaaccagcg tttgtccctt cgcttcaaat gggaccaacc
     3061 gcgaaccgca atcccaacca tttatgtaca gggttacgcg cgtgcgaacg aaatccctcg
     3121 tcgcatcgat tggcgagaca ggttggatgt gggcccgaac aattatttca ctgactcgcc
     3181 tcctgtcggc gcgctcagga atggtgtatt cgaggaaacc actgccgt
//
LOCUS       DASSFP010000488         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1931492, whole genome shotgun sequence.
ACCESSION   DASSFP010000488 DASSFP010000000
VERSION     DASSFP010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931492"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..567
                     /locus_tag="VFH91_10990"
     CDS             175..567
                     /locus_tag="VFH91_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6863568.1"
                     /translation="MEVEQTKRSSKKLVWIGRILSGLPVLFMVFSGVAKLLKPAQVIE
                     GFQKLGYPDHTILPLGVTELLCTILYVIPQTSVLGAILMTAYLGGATATHVRVGEPFF
                     APIILGMVAWLGLFLRDSRLRALLPLRS"
     gene            719..1630
                     /locus_tag="VFH91_10995"
     CDS             719..1630
                     /locus_tag="VFH91_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6863569.1"
                     /translation="MIERYTYQSALAASEMIHWRIEDIIGGEKRLNFDRPFMPESLAR
                     VEPLRFLNKEEKRTLNQIRGHAYLSIFGLVEEFILPFVLDHARPQLQSDDYRTRALLK
                     FAGEEAKHIQLFKLFRQEFEDGFGSKCEVIGPPEAIAKEILAHNPLAVAITTLHIEWM
                     VQKHYLESIKDDRQLDPQFKSLLKHHWLEEVQHAKLDTLMVATLAADKSEGEIMSSVE
                     EYLAIGSFIDSGLAHQVELDLQSLERACWLDLTDEQKVEFRKVQLQANRWSYLGSGMT
                     HERVLSTLASLTPKARQRVESVSAAFC"
     gene            complement(1685..2317)
                     /locus_tag="VFH91_11000"
     CDS             complement(1685..2317)
                     /locus_tag="VFH91_11000"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6863570.1"
                     /translation="MTRSNKIILGFVFTLLFVAACANPAADRSKAVTGEAATASPQSV
                     AQGEKYLINQQNSKIEFVGSKVVGSQQGSFEKFEGAINYAGQPEKSRVDIKIDMNSIK
                     ADEDGLTNHLKTPDFFDVAKYPQSTFTSTEIKPGGEKGATHTITGNLQLHGVTKAITF
                     PATIAVTTNAVTVDSNFSINRKDFGINYTGAADNLIRDEVVLTLKVRAVK"
     gene            complement(2314..2877)
                     /locus_tag="VFH91_11005"
     CDS             complement(2314..2877)
                     /locus_tag="VFH91_11005"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET6863571.1"
                     /translation="MRLKNPDSFILSGLSLALTLIVAGSAFSAQRALAGTQWRLDSFG
                     KAGDEKPILDGTSITIQFGTDGRASGSSGCNSYSGTYNAEGEHLSFGQMISTMRACVE
                     PKANQQEQTYMAALRAITRFTITESRLTLSDGGEVVLNFSSQSSSNGQGPGATTPVDL
                     LNSYFAAINGKHYGRAYALLKNSWRKR"
BASE COUNT          748 a          734 c          720 g          782 t
ORIGIN      
        1 ggcttccggc ggtctgcagt aaaacaaatt ccaatgtttc gcgtgaatca ggggcagcaa
       61 cgctgccctg attcaccacc tcgaggccgt cagcacttga aatcaatgcg taagatgtga
      121 gagttatagt tcattagctc aggcattttt aatcaacaaa ccggagagta accgatggaa
      181 gttgagcaaa cgaaacgcag ctctaaaaag ttggtttgga tcggacgcat cctcagcggc
      241 ctgcctgtgc tctttatggt cttcagcggg gtggccaagt tattgaagcc cgcacaggtc
      301 atagagggat ttcagaaact cggttatcca gatcacacaa ttttgccact tggcgtcact
      361 gaacttcttt gtacgattct ctacgtcatt cctcagactt cagtcctggg agccatccta
      421 atgacggctt atctcggcgg cgccactgcc acacatgtca gagtcggcga gccattcttt
      481 gcaccaatca tcctcggcat ggtcgcctgg ttgggactat ttttgaggga ttcgcgtctt
      541 cgcgctctac ttcccttgcg cagctagccg taaccacagc cacaactgaa tttataaatt
      601 ccgaaaagcg tccgttcaaa ttgattgctc ctcggtgacc tcagtttgcg cgttattcat
      661 ataaggcggc ggggggccgc tgaaacagtt ccggtgaaac gtcaagagga gtttccaaat
      721 gattgaaaga tatacctatc agtctgctct ggcggcatcg gagatgattc attggagaat
      781 tgaagacatc atcggcggcg agaaaaggct gaatttcgat agacccttca tgcccgagtc
      841 actcgcacgc gttgagcctt tacgttttct gaacaaagaa gagaagcgca ctctgaatca
      901 gatccgtggg catgcttacc tgagcatctt tggattggtt gaggagttca ttctaccgtt
      961 cgtgctggat cacgcgcgtc cacaactaca aagtgacgac tatcggacta gagcgttgct
     1021 gaagttcgct ggtgaagaag caaagcatat tcagttattc aaactcttcc gtcaggaatt
     1081 cgaagatggc ttcggatcca aatgtgaagt gattggacca cctgaagcga tcgccaaaga
     1141 gatcctcgca cacaatccac tcgctgtggc aattacaacc cttcatatcg agtggatggt
     1201 gcaaaaacac taccttgaaa gcatcaaaga tgatcgtcaa ctagatccgc agttcaagag
     1261 cctgttgaag catcactggc tggaagaggt ccagcacgct aagttggaca ctttgatggt
     1321 tgccactctt gctgccgata aaagcgaggg agagatcatg tcatctgtcg aggaatatct
     1381 ggccataggc agcttcatag atagtggact tgcccaccag gttgagctag atctacagag
     1441 ccttgaaagg gcttgctggc tcgacttaac ggacgagcaa aaggtggaat tccgcaaggt
     1501 ccaacttcag gcaaatcgct ggtcgtatct gggttcggga atgacccacg agagagttct
     1561 ttccacactc gcgtcgctga ctccaaaagc gcgacaacga gtggagagtg tctcggccgc
     1621 gttttgttaa gaaatagttt gaactctcat tgggccaccg cttgaaactg cggtggccct
     1681 ttgtttattt aacggctcga accttcaagg tcagcacaac ttcatcccga atgaggttat
     1741 cagctgcacc ggtataattg atgccgaagt ctttccggtt gatcgaaaag ttactatcaa
     1801 cggtaacagc attagtcgtt acagcgatcg tagcgggaaa cgtaattgct ttggtgactc
     1861 catgcaattg caaattacca gtaatcgtat gagttgcgcc cttttcacca cctggtttta
     1921 tctccgtcga ggtaaaggtt gattgagggt atttcgcgac atcaaagaag tccggcgttt
     1981 ttagatggtt tgtgagccca tcttcatctg ctttgataga gttcatgtcg atcttgatgt
     2041 ctacccggct cttctccggt tgacctgcat aattgatcgc accttcgaac ttttcgaagg
     2101 atccttgttg gctcccgacg acctttgatc caacaaactc tatctttgaa ttctgttgat
     2161 tgatcaaata tttctctccc tgggccacag actgtggcga tgccgttgcc gcctccccag
     2221 taactgcctt cgacctatca gccgcaggat tggcacaagc agcgacgaat aggagtgtaa
     2281 aaacaaaacc aagaatgatc ttattggacc gagtcatcgt ttcctccatg agttttttag
     2341 aagtgcgtac gctctaccat agtgcttccc gtttatcgcc gcgaaatatg agttcagcaa
     2401 gtcgacggga gtcgtagcgc cgggtccttg accgttcgac gaagattgac tactgaaatt
     2461 gagtactacc tccccgccgt cactcaacgt aagacgactt tcagttatcg taaatcgcgt
     2521 aatggctcgc aacgcggcca tgtacgtttg ttcttgttga ttagccttag gctcgacgca
     2581 agcacgcatc gtactgatca tttgtccaaa tgaaagatgt tctccttccg cgttgtaagt
     2641 gccgctgtag gaattgcacc ctgacgagcc actcgcgcga ccatcggtgc caaattgaat
     2701 tgtgattgag gtgccatcca aaatcggctt ttcgtctcca gcttttccga acgaatcaag
     2761 tcgccactga gtgccagcta gcgctctttg agcgctaaac gcactgccag cgacaatgag
     2821 cgtcaaagcc aggcttaggc cagaaagaat gaaagaatca gggtttttaa gtcgcatctt
     2881 cacttctcct attcttatgg ctgcctccta atcattgtcg atgactggaa caaagaaaag
     2941 tttggcttga aatcttaacc taaagttaca atccgattgt atgg
//
LOCUS       DASSFP010000489         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1931617, whole genome shotgun sequence.
ACCESSION   DASSFP010000489 DASSFP010000000
VERSION     DASSFP010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931617"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..688
                     /locus_tag="VFH91_11010"
     CDS             128..688
                     /locus_tag="VFH91_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6863572.1"
                     /translation="MPIRRSLISGMLALPFLISLNPHQTSGAPTLQTNARLVRQVRHE
                     LVTLPYYGVFDWLEFEVQPDNTIVLRGQVVRPTTKSDAEANVKDVDGVSRVVNQIEVL
                     PLSPQDDRLRRALYRTIYGSNSPLFRYAIQSIPPIHLIVNRGRATLKGVVANKSDSQL
                     AYIRARGVPGLFEVKNQLVVESEQPR"
     gene            786..1817
                     /locus_tag="VFH91_11015"
     CDS             786..1817
                     /locus_tag="VFH91_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET6863573.1"
                     /translation="MVEATKDYYQTLAVNRSASADEIKGAYRKLARKFHPDLNPGDKQ
                     AEERFKQLQEAYDVLSDVEDRKLYDQYGENWRAVKQGAGAPPPPGWEGGPPPGQRQEP
                     RVDFRDFDFGSFNSSPGGFDIFEEMFGRAGRARSQRSTRGRDVEAEISLSLDDAHRGA
                     RRTLQMQVADTCPTCHGTGTINDDQVCPTCGGAGQVLKPKTLEVNIPAGVRAGSIVRL
                     PGQGGAGANGSPPGDLYLHINLQPHPTFSIRGEDDLEIELTLAPWEAVLGTNLEVPTI
                     DGRVQLKIPTGAQPGQRLRLRGQGLNKRSGGRGDEYVRLKIVLPKSVSSEEKKLWEEL
                     RAASSLKPG"
     gene            1846..2163
                     /locus_tag="VFH91_11020"
     CDS             1846..2163
                     /locus_tag="VFH91_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HET6863574.1"
                     /translation="MRHNDVDMDEPLTCDIIAEITNTRRSLVVRLAQQGLLEVVTETS
                     EEPAEPILPRRTIMRLRRMKRLRHDLGVNFAGAAIILDLMERIRTLNRELTNARSATF
                     DDF"
     gene            2196..2747
                     /locus_tag="VFH91_11025"
     CDS             2196..2747
                     /locus_tag="VFH91_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET6863575.1"
                     /translation="MFVAMTNWWALVLRGVVAILFGVMTFIWPQITLATLVLLFGAYA
                     IVDGVFAIVAGVRAPREFARWWVLLIEGILSVIAGVIAFAIPGITALFLLGLIAGWAI
                     ITGVIEIVAAVQLRKEIQGEWLLALGGITSILFGVLLLLNPAAGALAVLWLIGIYAIL
                     FGIILVLLGFKLRGMLHGSATTV"
BASE COUNT          703 a          706 c          802 g          710 t
ORIGIN      
        1 gtagaacgat tgccacgcac tttcgagaat tgtctgctta agatacagaa caacactatt
       61 gggccgactg atgcgggcac tttcgccagg accgttgggc caacgagtga attttggagg
      121 ctcatcaatg ccaattcgca gatcactcat ttccgggatg ctggctcttc cctttttaat
      181 ttccctgaat ccacaccaga catccggcgc gccaactctt caaacaaacg cccgactcgt
      241 gcgacaggtc agacacgagc tggtaaccct tccttattac ggcgtgtttg attggctcga
      301 atttgaggtc caacctgaca acacaattgt cctgcgcgga caggtggtca ggcccactac
      361 aaaatcggat gccgaagcca atgttaagga cgtagacggc gtgtcgcggg ttgtgaacca
      421 gatcgaggtc ttgcctttat cgccgcagga cgatcgtttg cggcgcgcac tctatcgcac
      481 catctacggt tcaaactctc cgcttttcag atatgccatt cagtcaattc caccaattca
      541 cctaatcgtc aaccgtggac gcgccacgct taagggagtc gtcgctaaca agtccgactc
      601 gcaattagcc tacatcagag ctcgaggcgt tcccggtctg tttgaagtta agaaccagtt
      661 ggtggtagaa agcgagcagc caagatagac agtcgacgct agacagtcga cgctgacagt
      721 cgacgagatg ccgcatctcg gcgaatttca aaccaggtat atcagtctcg gactaatcac
      781 attcgatggt tgaagcgaca aaagattatt accaaactct cgctgtaaat cgctcagctt
      841 ccgcagatga gatcaaaggt gcgtaccgga agctggcccg gaaatttcat cccgacctga
      901 atccaggcga caagcaagca gaagaaagat tcaaacagtt acaggaagcg tacgacgtac
      961 tttcggatgt tgaagatcga aagctttatg atcaatacgg tgaaaactgg cgggccgtaa
     1021 aacaaggggc tggagctcca ccgccgccag gttgggaggg tggaccacca cccggtcaac
     1081 gacaagaacc gcgagttgat tttcgtgact ttgactttgg atccttcaat agcagtcctg
     1141 gcggtttcga tatctttgaa gagatgtttg gtcgcgccgg ccgcgcacgc tctcagcgca
     1201 gcactcgcgg tcgagatgtc gaagccgaaa tctcactcag tctggatgat gctcatcgcg
     1261 gagcacgacg cacgttacaa atgcaggttg cggacacgtg tcctacttgt catggaacag
     1321 gcacaatcaa tgacgatcag gtttgtccta catgtggtgg cgcgggccag gttctgaagc
     1381 caaaaactct ggaggtcaat attccggcgg gagttcgcgc tggatcgatt gtgcgattac
     1441 cgggacaagg cggagctggc gcaaatggtt cacctcccgg cgatctctac ctgcacatta
     1501 atcttcagcc tcatccaaca ttctcaattc gaggtgagga cgacctggaa atcgaattga
     1561 ctctcgctcc ctgggaagct gtcttaggta caaatctcga ggtgccgacg atcgatggaa
     1621 gagtgcaact gaaaattcca actggtgctc aaccgggcca acgactcagg cttcgggggc
     1681 agggtttgaa caaacgatcc ggaggtagag gcgacgagta cgtgcgcttg aagatcgttt
     1741 tgccaaaatc agtgagtagc gaagaaaaga aactatggga agagcttcgt gcagcttctt
     1801 ccttgaaacc tggatgaaaa ctactaaagt gggaaggtgc aagtcatgcg gcataacgac
     1861 gttgacatgg acgagccatt gacgtgcgat atcatagctg agattactaa cactcgaagg
     1921 tcgcttgttg ttaggctggc acaacaggga ttgttggaag ttgttacgga aacgagcgag
     1981 gagccggccg agccgatatt gcctcgcaga acgatcatga gactacgccg catgaaacga
     2041 ctacgtcatg acttaggcgt aaactttgct ggagccgcga tcattctgga cctgatggaa
     2101 cgcatacgaa ccttaaatcg tgaattgact aatgctcgta gtgcaacctt tgatgatttt
     2161 taactggtgc tgcttgagag atcggaggtg atcttatgtt cgttgcaatg acaaactggt
     2221 gggcgctggt acttagaggg gtggtggcca ttctcttcgg cgtgatgact ttcatctggc
     2281 cccagatcac gctggcaact ctggtcctac tatttggagc ttacgcgatt gtagatggtg
     2341 tgttcgcgat cgtcgccggt gttcgagctc cgagggaatt cgccagatgg tgggtcttat
     2401 tgattgaagg catcctgagc gtaatcgctg gagtgattgc cttcgcaata cctggcatca
     2461 ctgcattgtt cttgctcgga ttaattgccg gttgggccat aatcacgggt gtcattgaga
     2521 ttgtcgctgc ggtccaactg cgtaaagaga ttcaagggga atggttactg gcgctgggtg
     2581 gcatcacctc gatacttttt ggagttctac ttttgctgaa cccggcggcg ggagcgctgg
     2641 ccgtattatg gttgatcggg atctacgcaa tactctttgg gataatcctg gtattgctcg
     2701 gattcaagct acgaggaatg ttgcacggtt ctgccaccac ggtttgatct ttttttgtgc
     2761 tccgttgatt accggttgca agacggggcg cccacggacg gcggggcccc tacaagaatt
     2821 tattcgacaa gaccgttgcg tattgcgtag taggttagtt ccgcattggt gttgagttgc
     2881 atcttatcga gaatgcgacg gcggtaagta ctgattgtct t
//
LOCUS       DASSFP010000490         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1009
            k141_1487194, whole genome shotgun sequence.
ACCESSION   DASSFP010000490 DASSFP010000000
VERSION     DASSFP010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963175
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:03:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,206
            CDSs (total)                      :: 2,200
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 6
            tRNAs                             :: 6
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487194"
                     /isolate="SMAG_U1009"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="VFH91_11030"
     CDS             complement(<1..2074)
                     /locus_tag="VFH91_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6863576.1"
                     /translation="MNSPAKLLRATYVLVVITLLATVSYAQEGYKHPPKEVLDILNAP
                     VTPNALVSPTRDNVLLATSLRYPPLADLSQPMLRLAGRRINPASNSPHRLQYSVALTL
                     KRISDGSEIKIDLPAGAKISSVDWSEDGKRFSFLNTTANRVELWVGDTANGKIHTFKA
                     FAVNSAYGDPVTWMPDSRTLIVQLVPTRPAAPPTPLVPNEPNSQESSGRPGPVVTYED
                     LLKTPHDEDLFEYYATSQLAFVDSSSGKVNSIGKPAIIQSVDPAPDGQHLLVATVRRP
                     FSYLFTDSSFPRDVEVWDAKGKVVYKLASLPLADQVPIDGVITGPRFYRWRPTEPATL
                     TWVRALDDGNPKKKADFRDSILALKAPFTSTPVELYKTEQRFTGMTWGEKDGLLLISD
                     YDRDKRWLRTFMLTVDKAGSAPKLIWSRNQQDRYNDPGTPLTKTNASGQRVILQNGKD
                     IYLTGNGASPQGDRPFLDKFNLETLKAERLFRSDEKSYESFVALLSDDAKEFLTRRES
                     PTEAPNYILHGSTTRPFTQFADPTPQLRGITKQLVTYKRADGVQCSFTLYLPPNYKQG
                     TRLPTVVWAYPLEFNDANTAGQVAGSTQRATQIVGPSHLFFLLEGYAVLDNATMPVVG
                     DPETVNNTYVEQIVMSAKAAIDKAASMGVTDPDRVGVGGHSYGAFMTANLLAQSNLFR
                     AGIARSGAY"
BASE COUNT          520 a          541 c          608 g          561 t
ORIGIN      
        1 tgtatgcgcc gcttctcgcg attccggctc tgaacagatt agattgggcc aacaagttcg
       61 ccgtcatgaa cgctccataa ctgtgtccgc caaccccgac gcgatccgga tcagttacac
      121 ccattgaagc cgctttatcg atcgcggctt tggcgctcat cacaatctgc tcgacatatg
      181 tattgttgac agtttctgga tcgccaacga ccggcatcgt cgcgttgtct agtactgcgt
      241 agccttcaag caagaagaaa agatgtgatg gtccaacgat ctgtgttgcg cgttgagtcg
      301 agccggcgac ctgcccggca gtattcgcat cgttgaattc gagtgggtag gcccaaacta
      361 ccgtgggcag acgagtaccc tgtttatagt ttggcggaag ataaagagta aacgagcatt
      421 gaacaccatc tgctcgctta tatgtcacca attgtttcgt gatgccgcgc aactgcggag
      481 tgggatcggc aaactgcgta aacggtcttg tagttgatcc atggagaatg tagttcggtg
      541 cttcagtggg tgattcacgc cgcgttaaga actctttggc gtcatccgaa agcaacgcta
      601 caaaagactc gtaagatttc tcgtcactcc ggaacagtct ctcggccttt agtgtttcga
      661 gattaaactt atcgaggaat ggccggtcgc cctgcggcga agctccatta cctgtcagat
      721 aaatgtcttt cccattctgc aatatgaccc gctggccact cgcattcgtt ttcgtcaatg
      781 gcgtacctgg atcgttgtag cgatcttgct ggttccgact ccagattagt ttcggcgccg
      841 agccggcctt gtcgacagtc aacatgaacg ttctaagcca acgtttatcg cggtcgtagt
      901 cggagattaa gagcaacccg tccttttcgc cccaggtcat cccggtaaac cgctgctcag
      961 ttttatagag ttcgaccggt gtgctggtaa atggagcctt caacgccagg atgctatctc
     1021 gaaagtctgc cttctttttc ggattgccat catcgagtgc tcttacccac gtcagcgttg
     1081 ccggttccgt tgggcgccag cgatagaacc tgggtccggt gataacacca tcgataggaa
     1141 cttgatcagc aagaggtaaa cttgcgagtt tgtaaacgac cttccctttc gcatcccaga
     1201 cttcgacgtc tcgcggaaac gacgagtctg tgaacagata ggagaatggt cgacgaacgg
     1261 tggcgacaag caggtgctgg ccatcagggg ccggatccac ggattgaatg attgcgggct
     1321 tccctatcga gttgactttt ccacttgaag aatcgacgaa ggccaattgc gacgttgcgt
     1381 aatattcaaa aagatcttcg tcatgcggag tcttcaacag atcttcgtaa gttaccactg
     1441 gaccaggacg gcccgagctc tcctgtgaat tcggttcgtt aggaaccaga ggagttggcg
     1501 gtgcagcagg acgtgttggc acaagctgca cgatgagtgt gcggctgtcg ggcatccagg
     1561 tcactggatc gccgtaagct gagttgacgg caaatgcttt gaatgtgtga atctttccgt
     1621 tggccgtatc gccgacccaa agttcgactc gattcgcagt cgtattaaga aatgaaaagc
     1681 gcttgccgtc ctcgctccaa tcaactgagc tgatcttcgc gccagccggc aaatcgatct
     1741 ttatctcgct gccatctgaa attcttttga gtgtcagagc tactgaatac tgcaagcggt
     1801 gcggagaatt ggacgccgga ttgatacgtc tgcccgcaag ccggagcatt ggctgtgaaa
     1861 gatcggctaa cggtggatag cgcaaacttg ttgcgagtag cacgttgtcg cgagtggggc
     1921 tgaccagagc gtttggcgtc acgggcgcat tgagaatgtc gagcacttct ttcggcggat
     1981 gtttataacc ctcctgcgcg taagaaacgg tggcgagaag agtgattacg acgagaacgt
     2041 aggtagctct gagtaatttc gcaggcgagt tcataaaaaa gtttcctccg caaagaaaca
     2101 acaagttagg tggactggat tttttgtatt gagaaccgga gcgtccgaaa actattagag
     2161 ttagaggtgt atgtcaatag tcagacagat gctgtaatcg agttcgggag gaagggtgaa
     2221 gatgttttat
//
